LOCUS       DASVVN010000001         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_157787,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000001 DASVVN010000000
VERSION     DASVVN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157787"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF686_00005"
     CDS             <1..471
                     /locus_tag="VF686_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEX8660218.1"
                     /translation="ILGSPALWAPAREAIREGKLKLAWMYARRALRRAFSGKMRFSLD
                     GKAPRRAEALVLISPMISKVMDENTGLEAAAMNPADAAEAFRLAAHAVFSDWRQDPAV
                     TTRAIQGATVSARSRIPAVIDGEPILLSHEAKVKFIRKAFQALAPDPPAAEDSV"
     gene            471..1322
                     /locus_tag="VF686_00010"
     CDS             471..1322
                     /locus_tag="VF686_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8660219.1"
                     /translation="MGRILQFSDIHFGVEHKHACAAALEYAHANKPDLVLITGDITQQ
                     GFPDEFEAAGRWINQMPEPRFVIVGNHDVPYWSLFARLFSPWKAFEKATGHPAHDHQF
                     LSPTVMVRGVVTARGWQARMNWSKGVIDLMQTRKAAEALRQAPVGALRILACHHPLVE
                     MIGTPMTGDVKRGDAAALIFAEAGVDIIATGHVHVPFALPISLGDHCSYAVGCGTLSH
                     RERGAPPSFNQIEWDAHQITVTAIAWNGERFEPGQTWKLPRRQDTRKTATAPDPNVAG
                     EKEKAAV"
     gene            complement(1359..1523)
                     /locus_tag="VF686_00015"
     CDS             complement(1359..1523)
                     /locus_tag="VF686_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX8660220.1"
                     /translation="MLRWALIFLVLALVAGALGAGGVAGLSMNIAYVLFVVFLILLVV
                     HFITGRGRRI"
BASE COUNT          286 a          570 c          518 g          243 t
ORIGIN      
        1 atcctcggct cgccggccct ttgggctcct gcgcgggagg ccatccgcga gggcaagctg
       61 aaactggcct ggatgtacgc ccgtcgcgcc ctgcgccgcg ccttctctgg caagatgcgg
      121 ttctcgctgg acggcaaggc gccgcgtcgc gccgaggctc tggtgctgat cagcccgatg
      181 atctcgaaag tgatggacga aaacacaggc ctcgaggccg ccgccatgaa cccggccgac
      241 gcggcggaag ccttccgcct ggccgcacac gctgtattca gtgactggcg tcaggatccg
      301 gcggtgacca cgcgggccat ccagggcgcc acggtcagcg cccgctcccg aatccccgcc
      361 gtgatcgacg gcgagcccat tctgctgtcc catgaggcga aggtgaagtt catccgcaag
      421 gcattccagg ccctggcccc cgatccgccc gccgccgagg acagcgtcta gtggggcgga
      481 ttcttcagtt ctcggacatt catttcgggg tcgagcacaa acacgcctgc gccgccgcgc
      541 tggaatacgc ccacgccaac aagcccgatc tggtgctgat caccggcgac atcacccagc
      601 agggcttccc cgacgagttc gaggccgccg gccgctggat caaccagatg ccggagccgc
      661 gcttcgtcat cgtcggcaat cacgacgtgc cgtactggag cctgttcgcc cggctgttct
      721 caccgtggaa ggcgttcgag aaggctaccg gccacccggc ccacgaccac cagttcctca
      781 gccccacggt catggtgcgc ggcgtggtca ccgcgcgcgg ctggcaggcg cggatgaact
      841 ggtccaaggg cgtcatcgac ctgatgcaga cgcgcaaggc cgccgaggcc ctgcgtcagg
      901 ctcctgtcgg agccctgcgg atcctcgcct gtcaccatcc cttggtcgag atgatcggca
      961 cgcccatgac gggcgacgtc aaacgcggcg acgcggcggc cctgatcttc gccgaggccg
     1021 gggtggacat catcgccacc ggccacgtcc acgtgccctt cgccctgccc atcagcctgg
     1081 gcgatcactg ctcctacgcg gtgggatgcg ggaccctgtc gcatcgcgag cgcggggcgc
     1141 cgcccagctt caaccagatc gaatgggatg cgcaccagat cacggtgacc gccatcgcct
     1201 ggaacggcga acggttcgaa cccggccaga cctggaagct gccgcgccgt caggacacgc
     1261 gcaagaccgc caccgcgccc gatccgaacg tggcgggcga aaaggaaaag gccgcggtct
     1321 gatcgaccgc ggcctcgatt ttcgggactt gcgtccgtct agattcgtcg tcccctgccc
     1381 gtgatgaagt gcacgaccag caggatgagg aagacgacga acagcacata ggcgatgttc
     1441 atcgacagac cggcgacgcc cccggcgccg agggcgccgg cgaccagcgc cagtacgaga
     1501 aagataaggg cccagcgaag cataagcggt tccttccaga gcggttgatc cgaggagcga
     1561 caaccgccgc gcctgtttga tcaacgttcc ggaacgggcc tcgttccgcc tcagcgg
//
LOCUS       DASVVN010000002         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_131530,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000002 DASVVN010000000
VERSION     DASVVN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131530"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF686_00020"
     CDS             complement(<1..234)
                     /locus_tag="VF686_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX8660221.1"
                     /translation="MVGVLAGLVLTPDGRAWLRHPTIMLGDRAAASAPPAPTPVAPVV
                     PVTAAPVLPTVTPLDARLAQGPLRIGVFGDSMAD"
     gene            complement(363..776)
                     /locus_tag="VF686_00025"
                     /pseudo
     CDS             complement(363..776)
                     /locus_tag="VF686_00025"
                     /inference="COORDINATES: protein motif:HMM:NF021462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
     gene            complement(789..>1738)
                     /locus_tag="VF686_00030"
     CDS             complement(789..>1738)
                     /locus_tag="VF686_00030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8660222.1"
                     /translation="EARRRLDRDGPHDAAVFARNMAKRAIAEHAGAANEQHYELPPEF
                     FELCLGKRLKYSCCLYRDAGSTLGQAEEAALAETCVHAELADGQRILELGCGWGSLSL
                     WMAAQYPKASITAVSNSQGQRGHIEAQAAARGLTNLKVITADMNDFRAPTPMGEGYDR
                     IVSVEMFEHMANWRALLTKAHDWLKPDGRMFIHVFTHRDAPYRFDHTDSGDFIAQHFF
                     TGGIMPSRGLIRQFPDLFAVEREWTWNGVNYQRTADDWLANMDANADRVMELMRETYG
                     SDAELWRRRWRRFYLATAGLFGNDNGNTWAVSHYLLKPV"
BASE COUNT          293 a          614 c          541 g          290 t
ORIGIN      
        1 gtcggccatc gaatccccga acacgccgat ccgcagcggt ccttgggcaa gccgcgcgtc
       61 cagcggcgtc accgtgggca ggaccggcgc ggctgtgacc ggaacgaccg gagcgacggg
      121 cgtcggcgcg ggcggcgcgg aggccgcggc cctgtcgccc agcatgatcg tcggatggcg
      181 cagccacgcc cggccgtctg gcgtcagcac cagcccggcc agaacgccga ccatgaaggt
      241 cgccgtcaga gcatttatgc cagcccgcaa acccgtcccc cgactgaacc ctgttccgcc
      301 gagcctttta ccttatcggc gccggaccgc cacctcgcgc ccgtgtcacg ggaagaaaaa
      361 ggttaaccga agcggccctc ggcccatcgc gccgcgaaaa atccgcccag cgccgacacc
      421 gtcgtcagcg ccgttcccca gcacagatcg atgagcgtca gccgcatcga ccagacgttc
      481 agcgtcgcct gattggtcag gtcgtaggtc gcataggcca cgaaacccag caccgctccg
      541 ttgatcgcag ccctctgcca ggcgccctcg cgcaacgccg gctcgatggc gaggaagacg
      601 gttccggcga tcgagatcag atagaaggcg actgcggcct tcatgtccgg ctagtcggcc
      661 aggatcggac ccagcaccgg cttgtacagg cggttggcca tggtcgtcag ccagaccgcg
      721 tcgatcgcgg cgaaggtcag ccctgcaccc agataggcag ccacatattt gatcatcgct
      781 tcacctcttc agaccggctt gagcaggtaa tggctgaccg cccacgtgtt tccgttgtcg
      841 ttcccgaaca ggcccgccgt cgcgaggtag aaccgccgcc atcgccgccg ccacagctcc
      901 gcgtcagacc cgtatgtctc ccgcatcagt tccatgaccc ggtcggcgtt ggcgtccatg
      961 ttggccagcc agtcgtcggc cgttcgttgg tagttgacgc cgttccacgt ccattcccgc
     1021 tcgaccgcga acaggtcggg gaactgccgg atcaggcccc ggctgggcat gatgccgccg
     1081 gtgaagaaat gctgggcgat gaagtcgccg ctgtcggtat ggtcgaaccg gtacggcgcg
     1141 tcacggtggg tgaagacgtg gatgaacatc cgcccgtccg gcttcagcca gtcgtgcgcc
     1201 ttcgtcagca acgcgcgcca gttggccatg tgctcgaaca tctcgaccga gacgatccgg
     1261 tcgtagccct cgcccatcgg cgtcggcgcg cggaaatcgt tcatatcggc cgtgatgacc
     1321 ttcagattgg tcagcccccg cgccgccgcc tgcgcctcga tatgcccgcg ctgtccctgg
     1381 ctgtttgaaa ccgccgtgat cgaggctttc gggtactgcg ccgccatcca caggctgagg
     1441 ctgccccatc cgcagcccag ctcaaggata cgctggccat cggccagttc cgcatgaacg
     1501 caggtctcgg ccagcgcggc ttcctcggcc tggcccagcg tcgatccggc atcgcggtat
     1561 agacagcacg agtatttcag ccgcttgccc agacagagct cgaagaactc cggcggcaac
     1621 tcataatgct gctcattggc cgcgccggcg tgttcggcga tggcgcgttt ggccatgttg
     1681 cgggcgaaga cggccgcatc atgcgggccg tcccggtcca gacgcctgcg cgcctcgc
//
LOCUS       DASVVN010000003         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_394548,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000003 DASVVN010000000
VERSION     DASVVN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394548"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VF686_00035"
     CDS             <1..675
                     /locus_tag="VF686_00035"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070677 - rRNA
                     (cytosine-2'-O-)-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8660223.1"
                     /translation="VRLERCDDHASARAAELAIERLREGAVVALVSDAGTPLVSDPGF
                     VVARAVIAEGLPVHPVPGPSSLLAALCIAGLPADRVLFAGFLPPKSGARRAILEEVRT
                     ARQTLVFFESGPRLADSLTDMAAVLGPRPAAVARELTKLYEECVRGSLAELAVDPRCQ
                     SPKGEIVVVVGPGETEVASEADADAALAEAMTRLPPGEAAAEVSKALNLPRKPLYKRA
                     LEMQGR"
     gene            717..1112
                     /locus_tag="VF686_00040"
     CDS             717..1112
                     /locus_tag="VF686_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8660224.1"
                     /translation="MRVKRDWRVGLGSKAHRAGHGSEWLAAALLMLKGYQVLGFRLKT
                     RAGEIDILARRGRTLAVVEVKRRATLEAALVALTPDQFDRLVAAGRAVLRQRPSLADH
                     MLRIDMVALAPGRFPRHRRGVDAFGRGAQ"
     gene            1109..1924
                     /locus_tag="VF686_00045"
     CDS             1109..1924
                     /locus_tag="VF686_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX8660225.1"
                     /translation="MTRDDAIAILTEAGQAADDAFPLLEAAIACAIHDYPFRDPEPVR
                     TLANHAAERLAERITGESPDDALAETMAADLRLNGDLLHYDDPANTDIIDVAERRRGL
                     SVTLGVFYLDAARRAGVNAQGVDFPGHFLLRVETPEGPVALDPFSQGRLVLPSELTRR
                     ALRAGLTPHVADRLDVLMAPVSDRQALIRMQNVLFSRAIRAQDFEGAERSALRRALLD
                     PEDHRPWLDVAAAREKQGALTGALEALSHARTLDDPMEVTRRMSVDRVRLQLN"
     gene            1987..2931
                     /gene="gshB"
                     /locus_tag="VF686_00050"
     CDS             1987..2931
                     /gene="gshB"
                     /locus_tag="VF686_00050"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX8660226.1"
                     /translation="MLRVAIQMDPIEAVNIESDTTWLMMTTAQDRGHAQWVYDFRTLA
                     LEDGRLFCRARPVTLRQSVGDHVSFGDEVKLDLGADVDVILMRQDPPFDMAYVTATYL
                     LETVHPKTLVVNDPAEVRSAPEKLLVTRFPGLTPPTLISADPVALVDFHERHGDVVLK
                     PLHGAAGSGVVKLKAGDPNLEALVEIHMTGSRDPLVIQKFIPAVSKGDKRIILVEGEP
                     VGAINRIPAKDQVRSNLRVGGTAAPVELTDRDREICAAIGPTLRARGLLFVGIDVIGD
                     YLTEINVTSPTGAQQLKRFTGVDAAALMWNAIERKRAA"
     gene            3014..3760
                     /locus_tag="VF686_00055"
                     /pseudo
     CDS             3014..3760
                     /locus_tag="VF686_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
BASE COUNT          575 a         1361 c         1365 g          589 t
ORIGIN      
        1 gtccggctgg agcgctgcga cgaccacgcc tcggcgcggg ccgccgaact ggccatcgag
       61 cggctgcgcg agggggcggt ggtggcgctg gtgtcggacg cgggcacgcc cctggtcagc
      121 gatccgggtt ttgtcgtcgc ccgtgccgtc atcgccgagg gcttgcccgt ccatccggtt
      181 ccgggtccat ccagcctgct ggcggccctg tgcatcgccg gcctgcccgc cgaccgggtg
      241 ctgttcgctg gcttcctgcc gccaaaatcc ggcgcgcggc gcgccatact ggaagaggtg
      301 cgaaccgcgc gccagaccct ggtcttcttc gaaagcggcc cccgtctggc cgacagcctg
      361 accgacatgg ccgccgtgct cgggccacgc cccgccgccg tcgcccgcga actgaccaag
      421 ctgtacgagg aatgcgttcg gggctccttg gccgaactgg ccgtcgatcc gcgctgtcag
      481 tcgcccaagg gcgagatcgt cgtggtcgtg gggccgggcg agaccgaggt cgcctccgag
      541 gccgacgccg acgcggcgct ggccgaggcc atgacccgcc tgccgcccgg cgaggcggcc
      601 gcagaggtgt ccaaagccct gaacctgccg cgcaagccgc tctacaagcg ggcgctggag
      661 atgcagggcc ggtgacgccg ccgaagcccc tgacccgtct gaaagcgccg aaggccgtgc
      721 gcgtcaaacg ggactggcgg gtgggactgg gatcgaaggc gcatcgggcc ggtcacgggt
      781 cggaatggct ggcggcggcg ctgctgatgc tcaagggata tcaggtcctc ggcttccgcc
      841 tgaagacccg cgccggggag atcgacatcc tcgccagaag gggccggacg ctcgccgtcg
      901 tcgaggtcaa acgccgggcg acgctggagg ccgcccttgt cgccctgacc ccggatcagt
      961 tcgaccgcct tgtggcggcg gggcgggcgg tgttgcgcca aagacccagc ttggccgatc
     1021 acatgctgag aatcgatatg gtggcgctgg ctcccggacg atttccgcgc catcgtcgtg
     1081 gcgttgacgc cttcgggagg ggcgcccaat gacccgcgac gacgcgatcg ccattctgac
     1141 cgaggccgga caggccgcgg acgacgcctt tccgctgctc gaggcggcga tcgcctgcgc
     1201 catccacgac tatccgttcc gcgatcccga gcctgtccgg accctcgcca atcacgccgc
     1261 cgagcggctg gccgagcgca tcacgggcga aagccccgac gacgccctgg ccgagaccat
     1321 ggccgccgac ctgcgcctca acggcgacct gctgcactac gacgaccccg cgaacaccga
     1381 catcatcgac gtggccgagc gcaggcgcgg cctgtccgtg accctgggcg tcttctacct
     1441 cgacgccgcc cggcgggcgg gggtgaacgc gcagggcgtc gacttccccg gccatttcct
     1501 gctgcgcgtc gagacccccg aggggccggt ggcgctggac cccttcagcc agggccggct
     1561 ggtcctgccg tccgaattga cccgccgggc gctccgggcc gggctgacgc cgcatgtcgc
     1621 cgacaggctg gacgtgctga tggccccggt cagcgaccgt caggccctga tccggatgca
     1681 gaacgtgctg ttcagtcgcg ccatccgcgc ccaggatttc gagggcgccg aacgctcggc
     1741 cctgcgccgg gccctgctgg atcccgaaga tcatcgcccg tggctggacg tggccgccgc
     1801 gcgcgagaaa cagggcgcgc tgacgggcgc ccttgaggcc ctgtcgcacg cccggacgct
     1861 ggacgatccg atggaggtca cacgccggat gtcggtcgat cgggtccggc tccagctcaa
     1921 ctaggcttgc gccgccgccc ctcgctgccc attagccaag gctcaccgcc cttggaagga
     1981 cagccgatgc tcagagtcgc gatccagatg gaccccatcg aggcggtcaa catcgagtcc
     2041 gacaccacct ggctgatgat gaccacggcg caggaccggg gccacgccca gtgggtctat
     2101 gacttccgca ccctggcgct ggaggacggc cgcctgttct gccgcgcccg gccggtgacc
     2161 ctgcgccaga gcgtgggcga ccacgtcagc ttcggcgacg aggtgaaact ggatctgggc
     2221 gccgatgtcg acgtcatcct gatgcgccag gatccgccgt tcgacatggc ctatgtcacc
     2281 gcgacctatc tgctcgaaac ggtgcatccg aagaccttgg tcgtgaacga cccggccgag
     2341 gtccgctcgg ccccggaaaa gttgctggtg acgcgtttcc ccggcctgac cccgccgacg
     2401 ctgatcagcg cggaccccgt cgccctcgtc gatttccatg agcgccatgg cgacgtggtg
     2461 ctcaagccgc tgcacggcgc ggccggctcg ggcgtggtca agctgaaggc cggcgacccc
     2521 aacctcgagg ccctcgtcga gattcacatg acgggcagcc gcgatccgct ggtgatccag
     2581 aaattcatcc cggcggtgtc gaagggcgac aagcgcatca ttctggtcga gggcgagccg
     2641 gtcggggcca tcaaccgtat tccggccaag gatcaggtgc gctccaatct gcgcgtcggc
     2701 ggcacggccg cgccggtcga gctgacggac cgggaccgcg agatctgcgc cgccatcggg
     2761 ccgacgctgc gggcgcgggg cctgctgttc gtcggcatcg acgtgatcgg cgactatctg
     2821 accgagatca acgtcacctc gccgaccggc gcccagcagc ttaagcggtt cacgggcgtc
     2881 gatgcggccg cgctcatgtg gaatgcgatc gagcgcaaac gcgcggcctg acctcaggtt
     2941 gagtgtacgc ctgaattaag gttgcgttct atccgggttc gtgtttcgtt ctctccagtg
     3001 gcgggagggc gcggtggcgg ctcgggtggt gacggtcgcg ttcgaaggcg tcgaggcccg
     3061 gcgcgtcgat gtcgaggtgc agctggtcgg caatgaccag cagaccgtct tcgccatcgt
     3121 cggcctgccc gacaaggcgg tggccgagag ccgcgaacgg gtgcgcggcg ccttcgccgg
     3181 cataggcctc gccctgccgg cgcgccggat catcgccaat ctggcgccgg ccgacctgcc
     3241 caaggaaggc agccatttcg acctggcgat cgccttggcc ctgctggccg ccatgggcgt
     3301 gatcgccccc gatgcgctgg acggctgggc ggccgtgggc gagctcaatc tggacgggca
     3361 gatcgcgccc gtcggaggaa ccttgccggc ggcggtcgcg gcctcggcca tggggctggg
     3421 gctgatctgc ccggaggcca acggtcccga ggcggcatgg gcgggcgacg tcaacattct
     3481 ggccccgcgc tcgctcattg gactggtcaa tcacttcaag gggacacagg tgctgcgcgc
     3541 tccggagccc gggccggtgc ggatgggcgc ggcggtcccc gacctgcgcg aggtcaaggg
     3601 tcaggagagc gccaagcggg cgctggaaat cgcggcggcg ggcggccaca acctgctgtt
     3661 cgtgggcccg cccggctcgg gcaagtcgat gatggcggcg cgcctgcccg gccttctgcc
     3721 gcccctgacg ccgcaggagc tgctggagac ctcgatggtc cggtattagg tagcaaacac
     3781 accagcccgc gacaggcgca gagccaaccg ctaggctccc ccataagagg gagatactcg
     3841 tggcgctaaa gttcaatccg tttcagccca actccatggt cgttcccggc
//
LOCUS       DASVVN010000004         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_210520,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000004 DASVVN010000000
VERSION     DASVVN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210520"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF686_00060"
     CDS             complement(<1..447)
                     /locus_tag="VF686_00060"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX8660227.1"
                     /translation="MSLKRAFDPVVDANTRLLILGSLPGDASLKAAQYYAHPQNAFWR
                     LVGGVVGQDLAALPYPDRLETLKAAGVGLWDVIASAHRPGSLDAAIREAETADLNRLM
                     SGLPELKAVAFNGGTSARIGRRTLTLPIHVELIDLPSSSPAYTLSFA"
     gene            complement(449..784)
                     /locus_tag="VF686_00065"
     CDS             complement(449..784)
                     /locus_tag="VF686_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660228.1"
                     /translation="MIGLATLLLALLGPQQQTDTEGRTLIRTYKNPVNGTSRCLRNLP
                     PARRIAVQISCRVNGIGGPTRCTFQPETSPEQRYAAECVARGYRFNWEDGRPATGSTV
                     RFTVHLVTL"
     gene            complement(786..2084)
                     /gene="purB"
                     /locus_tag="VF686_00070"
     CDS             complement(786..2084)
                     /gene="purB"
                     /locus_tag="VF686_00070"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX8660229.1"
                     /translation="MISRYSRPAAVAIWSQETKYRIWFEIEAHAATRMAELGTIPASA
                     AEAIWAKGKDVVFDADRIDEIERTTKHDVIAFLTHVSEIVGEEARFLHQGMTSSDVLD
                     TCFAVQLARASDLLIEGVDRVLAALETRAKEHKFTPCVGRSHGIHAEPVTFGLKLAGY
                     HAEFQRAKRRLLAAKEEIATCAISGAVGTFANVDPAVEEYVAQKMGLAVEPVSTQVIP
                     RDRHAAFFAALGVVASSVERLAIEIRHLQRTEVLEAEEFFDKGQKGSSAMPHKRNPIL
                     TENLTGLARLVRSAVVPAMENVALWHERDISHSSVERGIGPDATIHLDFALHRLAGVV
                     ERLNVYPENMQKNLDLLGGLVHSQRVMLALTQAGVSREHAYAAVQENAMKVWRGEGRF
                     LDFLKADARVTLPAEQLEALFDLGYHTKHVDTVFARVFGG"
BASE COUNT          371 a          755 c          801 g          394 t
ORIGIN      
        1 cgcaaaggac agagtgtagg ccgggctgga cgacggtagg tcgatcagct cgacatggat
       61 cggcagagtc agagtgcgcc gcccgatgcg ggctgacgtg ccgccgttga aggcgacggc
      121 cttcagctcc ggcaaaccgg acatgagccg gttcaggtcc gcggtctcgg cttcacggat
      181 ggccgcgtcc agactgccgg ggcgatgggc gctggcgatg acgtcccaga ggccgacccc
      241 ggctgctttc agcgtctcca gacgatcagg atagggcagg gcggccagat cctgcccgac
      301 cacgccgccg acgagccgcc agaaggcgtt ttgcggatgg gcgtaatact gcgccgcctt
      361 cagcgaagcg tctccgggca ggctgccgag gatcaacagg cgcgtgttgg cgtcgacgac
      421 cgggtcgaag gcgcgcttca atgacacgtc agagggtcac cagatggaca gtgaagcgga
      481 ccgttgaccc ggttgccggt ctgccgtcct cccagttgaa cctgtagccc ctcgcgacgc
      541 attccgcggc gtagcgctgt tccggtgacg tctcgggctg gaacgtgcat cgggtggggc
      601 cgccgatgcc gttgacgcgg caggaaatct gcacggcgat ccggcgcgcg ggcggcaaat
      661 tcctcaggca ccggctcgtg ccgttgaccg gatttttgta ggtgcggatc agcgtgcgac
      721 cttcggtatc ggtctgttgc tgcggaccca gcaaggccag cagcagggtc gccagaccga
      781 tcatctcagc ctccgaacac ccgggcgaac accgtgtcga cgtgcttggt gtgatagccg
      841 agatcgaaca gcgcctccag ctgttccgcc ggcagggtca cgcgggcgtc agccttgagg
      901 aagtccagga agcggccctc gccgcgccag accttcatgg cgttctcctg cacggccgca
      961 taggcgtgct cgcggctcac gcccgcctgc gtcagggcca gcatcacccg ctgcgagtga
     1021 accagcccgc ccagcaggtc gaggttcttc tgcatgttct cgggatagac gttcagccgc
     1081 tcgaccaccc ccgccagccg gtgcagggcg aagtccagat ggatggtcgc gtccggcccg
     1141 atgccgcgtt cgacggacga atggctgatg tcccgctcgt gccagagggc gacgttctcc
     1201 atcgccggca caacggccga gcgcaccaga cgggccaggc cggtcaggtt ctcggtcagg
     1261 atcgggttgc gcttgtgcgg catggccgac gagcccttct ggcccttgtc gaagaattcc
     1321 tcggcttcga ggacttccgt gcgctgcagg tggcgaatct cgatggccag ccgctcgacc
     1381 gatgaggcga caacgccaag cgccgcgaag aaggccgcgt ggcggtcgcg cgggatgacc
     1441 tgggtcgaga ccggctcgac ggccagaccc atcttctgcg ccacatactc ttccaccgcc
     1501 ggatcgacgt tggcgaaggt gcccacggcg cccgagatgg cgcaggtggc gatctcttcc
     1561 ttcgccgcca gcaggcgccg cttcgcgcgc tggaactcgg cgtgatagcc ggccagcttc
     1621 aggccgaagg tcaccggttc ggcgtggata ccgtggctgc gcccgacgca cggcgtgaat
     1681 ttgtgctcct tcgcccgcgt ctccaacgcc gccagaacgc ggtcgacgcc ttcgatcagc
     1741 aggtccgagg cgcgcgccag ctgaaccgcg aaacaggtgt ccagcacgtc ggagctggtc
     1801 atgccctggt gcaggaagcg cgcctcctcg cccacgatct cggacacatg ggtcaggaag
     1861 gcgatgacgt cgtgtttggt ggtgcgttcg atctcgtcga tgcggtcggc gtcgaacacc
     1921 acatccttgc ccttggccca gatcgcctcg gcggcgctgg cggggatggt gcccagctcg
     1981 gccatccggg tggcggcgtg cgcctcgatc tcgaaccaga tccggtattt ggtctcctgc
     2041 gaccagatgg cgacggcggc ggggcgggaa tagcggctga tcatgggcgg gtcgtgtcgg
     2101 tcccgcgacc ggttgagtca agcgccgctt gtgccggacc gcttcaaact cgcccatcgg
     2161 cggctggcgg ccagcccgga gcggccttat tgcaggcgga ctgaatcgct ggaggggctc
     2221 atggaactga tcatcggacc gcgtctgtat tcgacctggt cgctgcggcc ctggctggtg
     2281 ctgaaacgct gcaaggccga cttcaccgtg cgcgagatgg a
//
LOCUS       DASVVN010000005         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_315711,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000005 DASVVN010000000
VERSION     DASVVN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315711"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF686_00075"
     CDS             complement(<1..680)
                     /locus_tag="VF686_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX8660230.1"
                     /translation="MIRQDASVLHFARRLSPSLLALAVAAGLAGPTLAQTAPPQPAIQ
                     PGAPVDAAPAPQAAPVQPERILINRILVRGNQRIDQTTVLSYLPIQPGDTVDPAVLDV
                     AVRTLTRTQLFADVQLGVQPNGDLIVEIVENPIINQVVFEGNGALSEDKLTEEVTLKP
                     RGIYTRARIQEDVGKIVELYRLSGRISATVTPKIVQLEQNRVDVVFEIDEGPETGVRA
                     ITFLGNTA"
     gene            complement(713..1927)
                     /locus_tag="VF686_00080"
     CDS             complement(713..1927)
                     /locus_tag="VF686_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease"
                     /protein_id="HEX8660231.1"
                     /translation="MTGFLGQTLLSIVSFLLVLTFIVTIHELGHFLVARAFGVKVDRF
                     SIGFGKTIVARTDKRGTEWRLAWLPLGGYVKFSGDLDASSVPDQAGLAELRQRVIAEN
                     GAGAERDYFHFKPIWQRALIVAAGPAANFLLAMVLFTVLFSVIGEQIVAPRVARVSPG
                     TPAAEAGFQPRDLIVAVNGQPVLEGAEVTRIVIMSAGDPLRFTVDRGGRQVFLTATPE
                     RRMEDDELAGRVRIARIGLEMAPLAQDYKFRRLNPLEAAGKGVDTIGDTVATTGTYLG
                     RIFTGKESGDLLNGPLGIAKAAGGVTRQAVAANPDPGYMAANLALTFLQFAAIVSIGI
                     GIVNLLPIPVLDGGHLLFYGYEAVARRPVPARVQEVGYRVGLALLAGLMLFATWNDLQ
                     KLSLFKFLGGLA"
     gene            complement(1951..3147)
                     /gene="dxr"
                     /locus_tag="VF686_00085"
     CDS             complement(1951..3147)
                     /gene="dxr"
                     /locus_tag="VF686_00085"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX8660232.1"
                     /translation="MIRRRVTVLGSTGSVGCSTLDLMDQIESAGTGVFEVAALTGGAN
                     IARLAEQARRWKPAIAVTADPARLQELRDALAGTGIETAAGDEAVIEAATRPVDWIMA
                     AIVGAAGLRSAWAAASTGATLALANKESLVCCGPALIQRVAGAGGRLIPVDSEHSAIH
                     QVFPHEAPGRVAKLVLTASGGPFRTLPRESLGAITPEQAVAHPNWSMGAKISVDSATM
                     ANKGLEAIEAAYLFDMPSDRIEVVVHPESIIHSMVEYVDGSTLAQMGPPDMKTPIAYA
                     LAWPDRIAWKAPRLDLAALGRLTFQAPDDERFPALKLARQALQAGGAAPTVFNAANEV
                     AALAFLDRRLGFLNIAAVVADTLSRMTAQGAVSCLNDACDAALEVDAQARRTAESIIR
                     GIAAAA"
     gene            complement(3144..3959)
                     /locus_tag="VF686_00090"
     CDS             complement(3144..3959)
                     /locus_tag="VF686_00090"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX8660233.1"
                     /translation="MAVKAGDIGLRAASAVVLAPAAVLAIWAGGFWFLGLMLAACALL
                     AVEWATMSAARAWRVMAGAVAFGLVAGVVAAHAQQLSLALVMLVFGAVAAGLFARNRG
                     QEALDAAYGVLYLGWPAVLLIWLRDVDSQTGLAWTVSAFAIAWASDIMAYLVGSLVGG
                     PKLWPRFSPNKTWSGFIGGLAAGTAAGALLATFLALGIGPVWGAVLGLAAALATMAGD
                     LWESALKRRYGVKDAGKLIPGHGGLLDRVDGLMFAVVVVACGRLLAGVFGAGA"
     gene            complement(3959..>4635)
                     /gene="uppS"
                     /locus_tag="VF686_00095"
     CDS             complement(3959..>4635)
                     /gene="uppS"
                     /locus_tag="VF686_00095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX8660234.1"
                     /translation="ARGLPRVMGHREGVQALKRTVQAAPALGITCLTVFGFSTENWSR
                     PAEEVSDLMGLVRTYVASDLKRLEREGVRVRILGRRAGLPADIAAIIDRTEATTAHND
                     RFTLQVAFNYGGRADIVDAAQRHVDRVLAGEATGPLDEKTLGEGLSTAGAPPVDVIVR
                     TSGEQRLSNFLLWEAAYAELVYQNVLWPDYGAEALADVVEQYRNRDRRFGGRATPGAA
                     PARAAG"
BASE COUNT          748 a         1698 c         1534 g          655 t
ORIGIN      
        1 aacgccgtgt tgccgaggaa ggtgatggcg cggacgccgg tttccgggcc ctcgtcgatc
       61 tcgaagacca cgtcgacgcg gttctgttcc agctggacga tcttgggcgt gacggtcgcc
      121 gagatccggc ccgacaggcg gtacagttcg acgatcttgc cgacgtcttc ctggatgcgg
      181 gcgcgggtgt agataccgcg cggcttcagg gtgacttcct cggtcagctt gtcttcgctg
      241 agcgcgccgt tcccctcgaa caccacctgg ttgatgatcg ggttttcgac gatctccacg
      301 atcagatcgc cgttgggctg cacgcccagc tgaacgtcgg cgaacagctg ggtgcgggtc
      361 agggtgcgca cggcgacgtc cagcaccgcg ggatcgaccg tgtcgcccgg ctggatcggc
      421 agataggaca ggaccgtggt ctggtcgatg cgctggttgc cgcgcacgag gatccggttg
      481 atcaggatcc gctcgggctg gaccggcgcg gcctggggcg ccggagcggc gtcgacgggc
      541 gcgccgggct ggatagcggg ctgcggcggc gccgtctggg ccagcgtggg gcccgccaga
      601 ccggcggcga ccgcaagggc cagcaagctc gggctcaggc gccgggcgaa gtgaaggacg
      661 gacgcgtctt gtcggatcat gcgggggacc atcgggggct ggcgtcggct cgtcatgcga
      721 gcccgccgag gaatttgaac aggctcagct tttgcaagtc gttccaggtg gcgaacaaca
      781 tcaatcccgc cagcaaagca agaccgacgc gataaccgac ctcctgaacg cgggcgggga
      841 ctggcctgcg cgccacagct tcatagccgt agaacagcag gtggccccca tccagaaccg
      901 gaatcggcag cagattaaca atgccaattc cgatcgaaac tatggcggcg aactgaagaa
      961 acgtcagcgc gaggttggcg gccatgtatc cgggatcggg attcgccgcc accgcctgac
     1021 gggtgacccc gcctgccgcc ttggcgattc cgagcggacc gttcagcagg tcgcccgatt
     1081 ccttgcccgt gaagatgcgg ccgaggtagg tgcccgtcgt cgcgaccgtg tcgccgatgg
     1141 tgtccacgcc cttgccggcg gcttccagcg ggttcaggcg gcggaatttg tagtcctgcg
     1201 ccagcggggc catctcgagc ccgatccgcg cgatgcgaac gcgtccggcc agctcgtcgt
     1261 cttccatgcg gcgttcgggc gtcgcggtca gaaacacctg ccggccgccg cggtcgacgg
     1321 tgaagcgcag cgggtcgccg gcgctcatga tgacgatgcg ggtgacctcg gccccctcca
     1381 gcaccggctg gccgttcacg gcgacgatca ggtcgcgcgg ctggaagccg gcctcggccg
     1441 ccggcgtgcc gggcgagacc cgggccaccc gcggcgcgac gatctgttcg ccgatcacgc
     1501 tgaacagcac cgtgaacagc accatcgcca gcaggaaatt ggccgccggc ccggcggcca
     1561 cgatcagcgc ccgctgccag atcggcttga aatggaaata gtcgcgctcg gccccggcgc
     1621 cgttctcggc gatgacccgc tgcctcaatt cggccagccc cgcctgatcc ggcacgctgg
     1681 acgcatccag atcgcccgag aatttcacgt acccgcccag cggcagccag gccagtcgcc
     1741 actcggtgcc gcgtttgtcg gtccgggcga cgatggtctt gccgaaaccg atcgagaagc
     1801 ggtcgacctt cacgccgaag gcgcgggcga cgaggaaatg ccccagctca tggatggtga
     1861 cgataaacgt cagcaccagc aggaaggaca cgattgagag caacgtctgg ccgagaaagc
     1921 ccgtcatgcc gataagggtc ccctctgccg tcaggctgcc gccgcgatgc cccggatgat
     1981 cgattccgcc gtccgccgcg cctgcgcatc gacctcgagg gccgcgtcgc atgcatcgtt
     2041 cagacaggaa accgccccct gggccgtcat gcgcgacaag gtgtcggcga cgactgcggc
     2101 aatattgaga aagcccagtc ggcggtcaag gaaggccagc gccgccacct cgttcgcagc
     2161 gttgaacacc gtgggcgccg cgccccccgc ctgcagcgcc tgccgggcca gtttcagggc
     2221 cgggaaacgc tcgtcgtcgg gagcctggaa ggtcagccgg cccagcgccg ccagatccag
     2281 ccgcggcgcc ttccaggcga tccggtccgg ccaggcaagg gcgtaggcga tcggcgtctt
     2341 catgtccggc ggccccatct gggccaaggt cgagccgtcc acgtattcga ccatgctgtg
     2401 gatgatcgat tccgggtgca ccacgacctc gatccggtcg gacggcatgt cgaacagata
     2461 ggccgcctcg atcgcttcca gccccttgtt ggccatggtg gcgctgtcga ccgaaatctt
     2521 ggcccccatg ctccagttgg gatgggccac ggcctgttcg ggcgtgatcg cgcccagact
     2581 ttcgcgcggc agggtgcgga acgggccgcc cgacgctgtc agcaccagct tcgccacccg
     2641 cccgggcgcc tcgtgcggga agacctgatg aatggccgaa tgttcggagt ccacggggat
     2701 cagccgcccg ccggcccccg cgacccgctg gatcagcgcc ggaccgcagc agacgaggct
     2761 ttccttgttc gccagggcca gtgtcgcgcc ggtcgaggcc gccgcccagg ccgagcgcag
     2821 tcccgcggcg ccgacgatgg ccgccatgat ccagtccacg ggccgggtcg ccgcctcaat
     2881 gaccgcctcg tcgccggccg ccgtctcgat gcccgtaccc gccagagcgt cgcgcaactc
     2941 ctgcagccgg gccggatcgg cggtcaccgc aatggcgggc ttccaacgac gcgcctgttc
     3001 cgccagccgg gcgatattgg ccccgccggt cagggctgca acctcgaaaa cgccggttcc
     3061 ggccgactcg atctgatcca tcaggtccag cgtcgaacag ccgaccgatc cggtcgatcc
     3121 cagcaccgtg acgcgccgcc ggatcacgcc ccggctccga acacgccggc cagcagccgc
     3181 ccgcaggcca ccaccacgac cgcgaacatc agcccgtcca cccggtccag caggccgccg
     3241 tgcccgggga tcagcttgcc cgcgtccttg acgccgtaac gccgcttcag cgccgactcc
     3301 cacaggtcgc ccgccatcgt ggccagcgcc gcggcgaggc ccagcacggc gccccacacc
     3361 gggccgatgc cgagggccag gaaggtcgcc agcagcgcgc ccgcggccgt gcccgccgcc
     3421 agaccgccga tgaagccgga ccatgtcttg ttcggcgaaa aacgcggcca cagcttgggt
     3481 ccgcccacca gactgccgac cagataggcc atgatgtccg aggcccatgc gatggcgaag
     3541 gccgacactg tccacgccag ccccgtctgg ctgtcgacgt cgcgcagcca gatcagcagc
     3601 accgccggcc agccgaggta gagcacccca taggccgcat ccagcgcctc ctgcccccgg
     3661 ttgcgcgcga acagccctgc cgccaccgct ccgaagacca gcatcaccag cgccagcgac
     3721 agctgctggg cgtgcgcggc caccacgccg gccaccagac caaaggccac ggccccggcc
     3781 atgacccgcc acgccctggc ggcgctcatc gtggcccatt cgaccgccag cagcgcgcag
     3841 gcggccagca tcaatcccag aaaccagaag cctcccgccc agatcgccag aacggcggcg
     3901 ggcgccagca cgacggccga ggcggccctg aggcctatgt cgcccgcctt gaccgccatc
     3961 agccggccgc gcgggcgggc gccgcgcccg gcgtcgcccg tccgccgaaa cgccggtcgc
     4021 ggttgcgata ctgctcgacc acgtccgcca gcgcctcggc cccgtagtcg ggccagagca
     4081 cgttctgata gaccagctcg gcgtaggccg cctcccacag caggaagttc gacagccgct
     4141 gctcgcccga ggtgcgcacg atcacatcga ccggcggcgc gccggcggtg gacaggccct
     4201 cgcccagcgt cttctcgtcg agcgggcccg tcgcctcccc ggccagcacc cgatccacat
     4261 ggcgctgcgc cgcatccacg atgtcggccc ggccgccgta gttgaaggcc acctgcaggg
     4321 tgaatctgtc gttgtgagcc gtcgtcgcct cggtccggtc gataatggcg gcgatgtcgg
     4381 ccggcaggcc cgcccggcgg cccagaatcc gcaccctgac gccttcgcgt tccagccgct
     4441 tcaggtcgct ggcgacatag gtgcgaacca gtcccatcag atccgagacc tcctccgccg
     4501 gccggctcca gttctcggtc gaaaagccga acaccgtcag gcaggtgatg cccagggcgg
     4561 gcgcggcctg caccgtgcgt ttcagcgcct gcaccccctc gcggtggccc atgacccgcg
     4621 gcaagccgcg cgcct
//
LOCUS       DASVVN010000006         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_184260,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000006 DASVVN010000000
VERSION     DASVVN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184260"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..535
                     /locus_tag="VF686_00100"
     CDS             41..535
                     /locus_tag="VF686_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660235.1"
                     /translation="MTRLALLAPLAAGLALWAAPAMADPASINVTVGPDVLEGADELG
                     QREVQEQASRLADVVRRTLAHRGALDGAEINLTLTDLKPNRPTIQQMVDRPGLDGIRS
                     ISIGGATITGHVTTSDGLIHPVAYDWYSTNISEVRGFSTWQDADRAYSRLANNLADGR
                     YNRR"
     gene            complement(532..1332)
                     /locus_tag="VF686_00105"
     CDS             complement(532..1332)
                     /locus_tag="VF686_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX8660236.1"
                     /translation="MTPSASSGPVVLLGCGRLGSAIVEGWLKTGAVAPCDLVILTPSE
                     KPAAERARALGARINPPASELEAARVLVLAVKPAKWRAAFEPLADALGAQTRLVSVMA
                     GVSADDIAQAAARPVARVMPTTAVAQARGVAALWSADSGTREIARTLFSPMAEFVNLD
                     AEAQIDVATAVAGSAPAFIYAFVEGLAGAGLAEGLPEEAARRLARGALRSAGAGVETD
                     ETLEALIARIASPGGTTRAGLDAMAAHGDLERAARSAVNAAVRRARSF"
     gene            complement(1338..1841)
                     /locus_tag="VF686_00110"
     CDS             complement(1338..1841)
                     /locus_tag="VF686_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8660237.1"
                     /translation="MDAVRPEEVDVPYDPLDVVEHVLVAENLPFDRTDEGDLAFALAG
                     DWKDYELWFAWRPEGDCLQLCCALDLQASKSRRAAAYELVGLINQRTWMGHFEVWAED
                     GEIVFRHSLALPMGERPTLAQAASMIDAAVEAADRYYPAFDFMIRGSKKPQEAIDACL
                     FETVGTA"
     gene            complement(1906..2193)
                     /locus_tag="VF686_00115"
     CDS             complement(1906..2193)
                     /locus_tag="VF686_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX8660238.1"
                     /translation="MQTRNPILDEFAKLTTGAMGLAQAAGEEAKTAFRAQTDRMVAEM
                     DLVRRDEFDALKAEITALRAEVAALKGAQKATKKPATGTSTASVPPQDAAG"
     gene            2382..>2502
                     /locus_tag="VF686_00120"
     CDS             2382..>2502
                     /locus_tag="VF686_00120"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX8660239.1"
                     /translation="MQFPEFDPVLLSIGPLDIRWYALAYVAGIVLGWWYASRLV"
BASE COUNT          408 a          875 c          816 g          403 t
ORIGIN      
        1 gatcatcttt cgtccgaccg gggggtcagt gaggatcaaa atgaccagac tcgctctcct
       61 tgcacccttg gccgccggcc tcgccctctg ggccgcgccc gcgatggccg accccgcctc
      121 catcaacgtc accgtgggcc ctgacgtgct cgaaggcgcc gacgaactgg gccagcgcga
      181 agtccaggag caggcctcgc ggcttgccga cgtcgtgcgt cggaccctgg cgcatcgcgg
      241 cgctctcgac ggcgccgaaa tcaacctgac gctgacggac ctgaagccca atcggccgac
      301 gatacaacag atggtcgaca ggcccggcct ggacggaata cgcagtatct cgatcggcgg
      361 tgcgaccatc accggtcacg tgacgacgtc cgacggtctg atccatccgg tcgcttacga
      421 ctggtattcg acgaacatct cggaagtgcg cggtttcagc acctggcagg acgcggaccg
      481 ggcctattcc cgtctggcca acaacctcgc cgacggccgc tacaacaggc gctagaacga
      541 ccgcgcccgc cgcacggcgg cgttgaccgc agaccgcgcc gcgcgctcca gatcgccatg
      601 ggccgccatg gcgtccagac ctgcgcgcgt ggtccctccc ggcgaggcga tacgggcgat
      661 cagcgcttcc agcgtttcgt ccgtctcgac cccggcccct gcagaacgca gggcgccccg
      721 ggccagacgc cgcgccgctt cctccggcag cccctccgcc agtcctgccc cggccagacc
      781 ctcgacgaag gcgtagatga aggctggagc cgatcccgcg acggccgtcg cgacatcgat
      841 ctgagcctcc gcgtccagat tcacgaactc ggccataggc gagaacagag tccgtgcgat
      901 ttcgcgcgtg ccggagtcgg cggaccacag ggcggcgacc ccgcgcgcct gcgccaccgc
      961 cgtcgtcggc atgacccgtg cgacggggcg ggcggcggct tgggcgatat cgtccgccga
     1021 aacccccgcc atgacggaaa ccagccgcgt ctgcgcaccc agagcatcgg ccagcggttc
     1081 aaaggccgcg cgccatttgg cgggtttcac cgccagcacc agcacccggg cggcttccag
     1141 ctcgctcgcg ggcggattga tccgcgcgcc caaggctcgc gcccgttcgg cggcgggctt
     1201 ttctgacggg gtgagaatga cgagatcgca cggcgccacc gcaccggtct tcaaccagcc
     1261 ctcgacgatg gccgagccca gccgtccgca gcccagaagg acgacggggc cggaggatgc
     1321 cgaaggggtc atggccgtca ggccgtgccg acagtctcga acaggcaggc gtcgatcgcc
     1381 tcctgcggct tcttcgatcc gcggatcatg aagtcgaagg ccgggtagta gcggtccgcc
     1441 gcctccaccg cggcgtcgat catggacgcg gcctgcgcca gcgtcgggcg ctcgcccatg
     1501 ggcagggcca gcgaatggcg gaagacgatc tcgccgtcct cggcccagac ctcgaaatgg
     1561 cccatccagg tgcgctggtt gatcaggccg accagctcat aggcggcggc acggcgcgat
     1621 ttggacgcct gaagatcgag ggcgcagcac agctgcaagc agtcgccttc gggccgccag
     1681 gcaaaccaca gctcatagtc cttccagtcg ccggccaggg cgaaggccag atcgccttcg
     1741 tcggtgcggt cgaacggcag gttttcggcc accaggacgt gttccacgac atccagcgga
     1801 tcgtagggaa catcgacttc ttcgggccgt acagcgtcca tcaggcactc tccgggcgcg
     1861 actcacgccc tgtcgtcaga cggtaggcgg gttcgcagat tcggctcaac cggctgcgtc
     1921 ctgaggggga accgacgctg tggacgttcc cgtggcaggc tttttcgtcg ccttttgagc
     1981 gcctttcagc gccgcgacct cggcgcgcag agcggtgatc tcggctttca gggcgtcgaa
     2041 ctcgtcccgg cggaccagat ccatttccgc gaccatgcga tcggtctggg cgcggaaggc
     2101 ggtcttggcc tcctcccctg cggcctgggc cagacccatg gcgcccgtcg tcagcttggc
     2161 gaactcgtcg aggatagggt tgcgcgtctg catctgcggg ctcctgctcg aaccaacgag
     2221 catcgttaac caaggttcac ccgatatggt gaatgaaacc ttgctgtcga tgcgacctat
     2281 ggcctgattt gggcgccggg aacgaggtcg ttgctgaaat ttcagggacg cgcccggcgg
     2341 cgcttgctaa accgcttccg gaacacccgt caggagcgcc cgtgcaattt cccgagttcg
     2401 atccggtgct gctcagcatc ggcccgttgg atatccgctg gtacgcgctg gcctatgtcg
     2461 ccggcatcgt gctgggctgg tggtacgcct cgcggctggt ga
//
LOCUS       DASVVN010000007         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_105366,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000007 DASVVN010000000
VERSION     DASVVN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105366"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..575
                     /locus_tag="VF686_00125"
     CDS             138..575
                     /locus_tag="VF686_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX8660240.1"
                     /translation="MTLKAPYDPDNIFARILRGEIPSVKVWEDDDVLAFMDVFPQSEG
                     HVLLISKTSTARTLLEIEPDVLARLIAATQRTARAVEKALNPEGFQIMQFNGEAGGQT
                     VFHLHFHIIPRWTDRPMKGHGHAPMADTEALRALADRIAAALD"
     gene            complement(572..>1663)
                     /locus_tag="VF686_00130"
     CDS             complement(572..>1663)
                     /locus_tag="VF686_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8660241.1"
                     /translation="KSAIARFGEAVEAAFGVSLVRIRARALMTAMIIVVMFGGVTLVL
                     WLGAQDVIAGRMSPGALLQFVLLSVFAAGAVGALGESWGDVQKAAGAMERIDELMRSE
                     AAIRAPETPRVLPEPPTGEVSMSAVQFAYPGRPDLPALKGFSLTVRPGETVALVGPSG
                     AGKSTVFRLLLRFYDPQSGVVSVDGIDVREADPVAVRGRFAWVSQEAPLFSGSATQNI
                     RFGREEADETAIRAAAEEAQALTFVEALPQGFDTPLGERGKSLSGGQRQRMAIARALV
                     RDAPILLLDEATSALDAENERLVQAALDQAMENRTTIVIAHRLATVLRADRIVVMEDG
                     KVVQEGTHRALMAQGGLYAKLAELQFRAD"
BASE COUNT          267 a          669 c          506 g          221 t
ORIGIN      
        1 aacccggaac ccaggcgact ggactcggac gcgtggctcg gctgaagtcg gcggatgctc
       61 ccctgggctc cgggttcgct cttcgagcgc cccggaatga cgatggaaat gacagcccga
      121 tcgaaagcta ccccgcgatg accctgaagg cgccctacga ccccgacaac atcttcgcca
      181 gaatcctgcg cggcgagatc ccctcggtga aggtgtggga ggacgacgac gtcctcgcct
      241 tcatggacgt cttcccgcag tcggaaggcc atgtcctgct gatctcgaaa acctccaccg
      301 cccgcaccct gctcgagatc gagccagacg tcctcgcccg cctgatcgcc gcgacgcagc
      361 gcacggcgcg cgccgtggaa aaggcgctca accccgaggg cttccagatc atgcagttca
      421 acggcgaggc cggcggtcaa accgtcttcc acctgcattt ccacatcatc ccgcgctgga
      481 ccgaccgccc tatgaagggc catggccacg cccccatggc cgacacggaa gccttgcgcg
      541 ccctcgccga ccgtatcgcc gcggcgctgg actagtcggc gcggaactgc agctcggcca
      601 gtttggcgta gagtccgccc tgcgccatca gcgcccggtg cgtaccctcc tgcaccacct
      661 tgccgtcctc catcaccacg atgcggtcgg cgcgcaggac ggtggccaga cggtgggcga
      721 tgacgatggt ggtgcggttc tccatcgcct gatccagcgc ggcctgcacc agacgctcgt
      781 tctcggcgtc cagcgccgag gtcgcctcgt ccagcagcag gatcggcgcg tcgcggacca
      841 gcgcccgggc gatggccatg cgctggcgct gcccgccgga caggctcttg cctcgctcgc
      901 ccagcggcgt gtcgaacccc tgcggcaggg cctccacgaa tgtcagcgcc tgcgcctcct
      961 ccgcggcggc gcggatcgcg gtctcgtccg cctcctcccg cccgaagcga atgttctggg
     1021 tggccgaacc cgagaacagc ggcgcctcct gggacaccca ggcgaaccgc ccgcgcacgg
     1081 cgaccggatc ggcctcccgc acgtcgatcc cgtccaccga caccacgccc gactgcggat
     1141 cgtagaaccg cagcaacagc cggaagaccg tgctcttgcc cgcccccgac ggcccgacca
     1201 gcgccaccgt ctcgcccggc cgcacggtca gcgaaaaccc cttcagcgcc ggcaggtccg
     1261 gccggcccgg ataggcgaac tgcaccgccg acatcgacac ctcgcccgtc ggcggctcgg
     1321 gcaggacccg cggcgtctcc ggcgcccgga tggcggcctc gctgcgcatc agctcgtcga
     1381 tccgctccat ggcgcccgcc gccttctgca cgtcgcccca gctctcgccc agcgcgccca
     1441 cggccccggc cgcgaacacc gacagcagca cgaactgcag cagcgccccc ggcgacatcc
     1501 gcccggcgat gacatcctgc gcgcccagcc acagcaccag ggtcacgccg ccgaacatca
     1561 ccacgatgat catcgccgtc atcagggccc gcgcccggat ccggaccagc gacacgccga
     1621 acgccgcctc gaccgcctcg ccgaagcggg cgatggcgct ctt
//
LOCUS       DASVVN010000008         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_447498,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000008 DASVVN010000000
VERSION     DASVVN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447498"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VF686_00135"
     CDS             complement(<1..94)
                     /locus_tag="VF686_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerase"
                     /protein_id="HEX8660242.1"
                     /translation="MRQWTVDAFASAPFKGNPACVVEPFDRWPSD"
     gene            complement(94..675)
                     /locus_tag="VF686_00140"
     CDS             complement(94..675)
                     /locus_tag="VF686_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8660243.1"
                     /translation="MSDLEARLVGIVRADPGLMHVLKGVRDQDLPDWRIFSGAVYQSV
                     WNALTGRPAGYGVRDYDVGYYDPDTSWDAEDVVIKRVAAAFDEPFRSTVEVRNQARVP
                     IWFPGHFGEPYEPLSGTDEALERFVAPAFAVGVRLEKDDAISVAAPLGLDDMFALTLR
                     PNPNRGVAKDWPRVIEKALARWPELQVIEPEPA"
     gene            complement(672..1475)
                     /gene="thiD"
                     /locus_tag="VF686_00145"
     CDS             complement(672..1475)
                     /gene="thiD"
                     /locus_tag="VF686_00145"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX8660244.1"
                     /translation="MTSPLGRVLIIAGSDSGGGAGVQADIKAVTAMGGYAATAITAIT
                     VQNTLGVHGVHPLPLDLIEAQARAVLDDIGADAIKTGMLGSREVVERVAAILDGCGAP
                     AVVDPVMIAKGGHSLLADDAVAAVRSLMIPRAALLTPNAPEAEALTGLDVADLDGQRR
                     AGEFLLGLGAQAVLMKGGHIPGDTVVDVLVTPTGETLLEGPRVDTRHTHCTGCTLASA
                     CAAGLARGLPLEAAVAEAWAYVAEAIRRAPGLGGGHGPLDHGWPLRRDQ"
     gene            1559..1870
                     /locus_tag="VF686_00150"
     CDS             1559..1870
                     /locus_tag="VF686_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8660245.1"
                     /translation="MHAALQALSEPHRMSILSMLAEGERPAGDFVDALPISQPTVSKH
                     LSVLREAGLVTVRKDAQRRMYRLNPAPLAELDVWLARYRSLWAGKLDALEAHLDKEFP
                     Q"
     gene            1867..2421
                     /locus_tag="VF686_00155"
     CDS             1867..2421
                     /locus_tag="VF686_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8660246.1"
                     /translation="MTGTTDRVRLDADAGTFRRIEGGWELRFERRLRHSPERVWTALT
                     TGDGLACWLAEADIDARPGGRMELNFRQPDHDFMPDVPERRRQSNEVLVVRPFTRFEH
                     TFGGNPESVVSWRLEPDGDGTHLTLVHRVPESWAGARSNVLSGWHHHMEGLDDAVRGV
                     LHPWSWDRWFALRDTYAAGLEGAA"
     gene            2418..2891
                     /locus_tag="VF686_00160"
     CDS             2418..2891
                     /locus_tag="VF686_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8660247.1"
                     /translation="MTAPVASAGIMIRRPVAEVFEAFADPAATSRFWFTRGSGRLEPG
                     ARVRWDWEMYGVGTDVLVKAVEPGRRILIDWDTADDPTEVEWLFEPRGEGSVVKVENR
                     GFEDNPEGVAKALDSTGGFNLVMAAAKIWLEHGIDPRIIEDTHAAARVPDWKGRS"
     gene            2888..>3289
                     /locus_tag="VF686_00165"
     CDS             2888..>3289
                     /locus_tag="VF686_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8660248.1"
                     /translation="MTGPAVRVEKHFRHPRERVFDAFLNPARAGDWLFHTPDGVMEKT
                     DYDPRPGGAFAIFERRGADLARHFGRFVEIDRPERILFDFWVDEAPDEPTRVTVTFAT
                     SGDGCVVVLSHDLAPAWAAYADRSIAGWTMIL"
BASE COUNT          501 a         1137 c         1132 g          519 t
ORIGIN      
        1 cgtcggacgg ccagcggtcg aacggctcga ccacgcacgc gggattgccc ttgaagggcg
       61 cggaggcgaa ggcgtcgacg gtccactggc gcatcaggct ggctccggtt cgatgacctg
      121 cagttccggc cagcgggcca aggccttttc gatgacgcgc ggccagtctt tcgccacgcc
      181 gcggttcgga ttgggccgca gcgtcagggc gaacatgtcg tccagtccca gcggcgcggc
      241 gacactgatc gcatcgtcct tctccagccg cacgccaacg gcgaaagccg gagccacaaa
      301 gcgttccagc gcctcgtcgg tgcctgacag cggctcatag ggctccccga agtggcccgg
      361 aaaccagatc ggcacccggg cctgattgcg cacctcgacc gtgctgcgga acggctcgtc
      421 gaaggcggcc gccacgcgct tgatgaccac gtcctccgcg tcccagctgg tgtccggatc
      481 gtagtagccg acgtcgtagt ctcgcacgcc gtagccggcg ggccggcccg tcagggcgtt
      541 ccagacgctc tggtagacag cgcccgagaa tatccgccag tctggcaggt cctgatcccg
      601 cacgcccttc agcacatgca tcagaccggg atcagcgcga acgatgccga ccagccgcgc
      661 ttcaagatcg ctcattggtc gcgcctcagc ggccagccgt gatccaacgg tccatgcccg
      721 cctcccagcc ccggcgcgcg gcggatggcc tcggcgacat aggcccaggc ctcggccacc
      781 gcggcctcga gcggcaggcc cctggccagt cccgccgcgc aggcgctggc cagggtgcag
      841 ccggtgcagt gggtgtggcg ggtgtcgacg cgcgggccct ctagcagggt ttcgccggtg
      901 ggcgtgacga ggacgtcgac gacggtgtct cccggaatat gcccgccctt catcagcacc
      961 gcctgtgcgc cgaggccgag aaggaattcc ccggcccggc gctggccgtc gaggtcggcc
     1021 acatccaggc cggtcagggc ctcggcctcc ggcgcgttgg gggtcagcag ggcggcgcgg
     1081 gggatcatca gcgacctgac cgccgcgacg gcgtcgtcgg ccagcagact gtgccccccc
     1141 ttggcgatca tgaccggatc gacgaccgcc ggcgcgccgc atccgtcgag aatggcggcg
     1201 acccgttcca ccacctcgcg cgatcccagc atccccgtct tgatcgcgtc ggcgccgata
     1261 tcgtccagca cggctcgcgc ctgggcctcg atcagatcca gcggcagcgg atggacgccg
     1321 tggacgccga gggtgttctg caccgtgatg gcggtaatgg ccgtcgccgc atagccgccc
     1381 atggcggtga ccgccttgat gtcggcctgc acccccgccc cgccgcccga atccgagccg
     1441 gcgatgatca ggacgcgtcc gaggggggaa gtcatccgcg ctggatgacc aatccgggcc
     1501 aaaacggcaa gccggatcgt atattgtatt tcatctatat tgccggtaaa cgattttcat
     1561 gcacgctgcc cttcaggccc tgtcggaacc ccaccggatg tcgatcctgt cgatgctggc
     1621 cgagggagaa cggcccgccg gagacttcgt cgacgccctg ccgatcagcc agccgacagt
     1681 gtcgaaacac ctgtcggtgc tgcgcgaggc cggactggtg accgtgcgca aggacgccca
     1741 gcggcggatg tatcgcctca accccgcgcc gctggccgaa ctggatgtct ggctggcccg
     1801 ttatcgcagc ctgtgggcag gcaagctgga cgcgctggag gcgcatctcg acaaggagtt
     1861 cccgcaatga ccgggactac ggatcgcgtg cggctggacg ccgacgccgg aaccttccgc
     1921 cgcatcgagg gcggctggga actgcgcttc gagcgacggc tgcgccattc gcccgaacgc
     1981 gtgtggacgg cgctgaccac cggcgacggt ctggcctgct ggctggccga ggccgatatc
     2041 gacgcgcggc cgggcggtcg tatggagctg aatttccgcc agcccgatca cgacttcatg
     2101 ccggacgtgc cggaacggcg ccggcagtcg aacgaggtgc tggtggtccg gccctttacc
     2161 cgcttcgagc acaccttcgg cggcaatccg gagtcggtcg tgtcgtggcg gcttgagccc
     2221 gacggcgacg gcacgcatct gacgctggtg catcgggtgc cggaatcgtg ggccggcgca
     2281 cgcagcaatg tgctcagcgg ctggcatcat cacatggagg gcctggacga cgccgtgcgc
     2341 ggcgtactcc atccctggtc gtgggaccgc tggttcgccc tgcgcgacac ctacgccgcg
     2401 gggctggagg gcgcggcgtg accgccccgg tcgcctcggc cggcataatg atccggcgtc
     2461 cggtcgcgga ggttttcgag gccttcgccg atccggcggc gacgtcccgg ttctggttca
     2521 cccggggttc gggacggctg gagcccggcg cgcgggtccg ctgggactgg gagatgtacg
     2581 gcgtcggcac cgatgtgctg gtcaaggcgg tcgaacccgg ccgccgcatc ctgatcgact
     2641 gggacaccgc tgacgacccg accgaggtcg agtggctgtt cgagcctcgc ggcgagggct
     2701 cggtcgtcaa ggtcgagaac cgcggcttcg aggacaatcc ggagggcgtg gcaaaggcgc
     2761 tggattccac cggcggcttc aaccttgtga tggcggccgc caagatctgg ctcgagcacg
     2821 ggatcgaccc gcgcatcatc gaggacaccc atgctgctgc tcgcgtgccg gactggaagg
     2881 ggcgatcatg accggtcccg cagttcgcgt cgaaaagcat ttccgccatc cgcgagagcg
     2941 ggtgttcgac gcctttctca acccggcgcg ggccggtgac tggctgttcc acacccctga
     3001 cggcgtgatg gaaaaaaccg actatgatcc ccgtccgggc ggcgcattcg ccatcttcga
     3061 gcgacgcgga gccgacctgg cgcggcattt cggccgcttc gtcgagatcg accggcccga
     3121 gcgcatcctc ttcgacttct gggtcgacga ggcgccggac gagccgaccc gcgtcaccgt
     3181 gaccttcgcg acttccggcg acggttgtgt cgtcgtcctg agtcacgacc tggccccggc
     3241 ctgggccgca tacgcggatc gcagcatcgc cggctggacg atgatcctc
//
LOCUS       DASVVN010000009         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_263339,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000009 DASVVN010000000
VERSION     DASVVN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263339"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1620
                     /locus_tag="VF686_00170"
     CDS             124..1620
                     /locus_tag="VF686_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660249.1"
                     /translation="MTSSRPFARIASLIGGAAAMAAAVGAFVLTDASTARAQVAAKVE
                     SQTRPNFGILLDPPTRSYRNRGSSHRRYDYGRHRPDWRPGYSGGYPRGEEIVLVDCGG
                     NPGTGAVEDAVRRVRPGGTLVIRARGGACVGWLNIDKPLTIIGESGFDPRDWDRNPTP
                     TLIAPDGLPCMTVAQGVRVEIRDMMFASPHGGDAACIVGYGADIVMNRVGFRHAGDEA
                     AIYVDGGLLDIRNAVIDAQTIAPAIVADGAVLTAWEMVVTGAQSGLELIPGAGQTSRI
                     GSTTLKGTGAPNAFGPRSIGVVVRSAREYGRVEIENTAIVGYVEGVAVEGASVQIHNS
                     RIGRSDKGVVLYNGELRLADSRVRATTVGVAAASGHAVIVNNVFAGVREVIYKEAQAT
                     VEASGNRVWSRYACQPQFRDRYRGRYEPYWRPGDGWTCEINSYPRDWWDDEDGLLGLP
                     YQDDGYQPDGYGDYQDGQGWYDRDGRYIPDSRYQGDDRWSRGGGVWGR"
     gene            1750..1929
                     /locus_tag="VF686_00175"
     CDS             1750..1929
                     /locus_tag="VF686_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660250.1"
                     /translation="MSLPAESVADVCVLDAPDDGAAVRKAHEVARGWPIDATVSLYHG
                     ERLVEVLEGELRKAA"
     gene            1970..2146
                     /locus_tag="VF686_00180"
     CDS             1970..2146
                     /locus_tag="VF686_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660251.1"
                     /translation="MDLQAQIVEAIRLELARQSGGAGAALNFSDDEGRAKVNGSLDLE
                     ALAMAISGAVAGGP"
     gene            complement(2221..2445)
                     /locus_tag="VF686_00185"
     CDS             complement(2221..2445)
                     /locus_tag="VF686_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660252.1"
                     /translation="MRARISIRSLAAAATFAASGLLTVSPAAAAPGDYCLYASGEVYC
                     GTEEFIYENDIRLPNCVFRWEQAQWDCPWE"
     gene            complement(2617..2689)
                     /locus_tag="VF686_00190"
     tRNA            complement(2617..2689)
                     /locus_tag="VF686_00190"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2654..2656),aa:Ile,seq:cat)
BASE COUNT          486 a         1036 c         1093 g          447 t
ORIGIN      
        1 aggccgtggc cctgaggatc gattcagccc tgtcgggctt cggcccctcg gccggccgcc
       61 tgcaggccgt gctggcccag gtctcccgtt cgcagcaacg ccgctaagga gggcgtgagg
      121 atcatgacga gctccagacc cttcgcccgg atcgccagcc tgatcggggg cgccgccgcc
      181 atggccgccg ccgtcggcgc cttcgtcctg accgacgcct cgacggcgcg ggcccaggtc
      241 gccgccaagg tggaaagcca gacgcgaccc aatttcggca tcctgctgga cccgccgacg
      301 cgcagctatc gcaaccgcgg ttcgtcgcac cggcgttatg actatggccg gcaccggcca
      361 gactggcggc ccggttattc cggcggctat ccgcgcgggg aggagatcgt actggtcgac
      421 tgcggcggca acccaggaac gggcgccgtc gaggatgcgg tgcgccgggt ccgtccgggc
      481 ggcacgctgg tcatccgcgc ccgcggcggc gcctgcgtcg gctggctgaa catcgacaag
      541 ccgctgacca tcatcggcga gagcggcttc gatccgcgcg actgggaccg caatccgacg
      601 ccgaccctga tcgcgcccga cggcctgccg tgcatgacgg tggcgcaggg ggtgcgggtc
      661 gagatccgcg acatgatgtt cgcctcgccg cacggcggcg acgcggcctg catcgtcggc
      721 tatggcgcgg acatcgtcat gaaccgggtc ggcttccgcc atgcggggga cgaggcggcc
      781 atctatgtgg acggcggcct gctggatatc cgcaacgccg tgatcgacgc ccagaccatc
      841 gccccggcca tcgtcgccga cggcgcggtc ctgaccgcct gggagatggt ggtgacgggc
      901 gcccagtcgg ggctggagct gattccgggc gcgggccaga cctcgcgcat cggttcgacg
      961 acgctgaaag ggacgggcgc ccccaacgcc ttcggaccgc gctcgatcgg ggtggtggtg
     1021 cggtcggcgc gcgaatatgg ccgggtcgag atcgagaaca ccgccatcgt cggctatgtc
     1081 gagggcgtgg cggtcgaggg ggcctcggtc cagatccaca acagccgcat cggccgctcg
     1141 gacaagggcg tggtgctgta caatggcgag ctgcggctgg ccgacagtcg ggtgcgcgcg
     1201 accacggtcg gcgtcgccgc ggcctcgggc catgcggtga tcgtcaacaa cgtcttcgcc
     1261 ggcgtgcgcg aggtcatcta caaggaggcc caggccacgg tggaggccag cggcaaccgc
     1321 gtctggtcgc gctacgcctg ccagccccag ttccgcgacc gctaccgcgg gcggtacgag
     1381 ccctactggc ggcccggcga cggctggacc tgcgagatca actcctatcc gcgcgactgg
     1441 tgggacgacg aggatggcct gctgggcctg ccctatcagg acgacggcta tcagcccgac
     1501 ggctacggcg actatcagga tggccagggc tggtacgacc gcgacggccg ctacatcccg
     1561 gacagccgct atcaggggga cgaccgctgg agccggggcg ggggcgtctg ggggcggtag
     1621 gcggccagcg gctttcaagc ctctgatctc cgtgaccaaa tgtcagatag tcgtcacagg
     1681 aacgagtcgc gcggcgggta gtttagcggc ctccagagag aggcttgaat ggactgctat
     1741 ttctgcatcg tgagcctgcc ggccgagtcc gtggcggacg tttgcgtgct cgacgcgccc
     1801 gacgacggcg cggcggtgcg caaggcgcat gaggtggcgc gcggctggcc catcgacgcg
     1861 acggtcagcc tctatcatgg cgagcgtctg gtcgaggtgc tggaaggcga gctccggaag
     1921 gcggcctgac gcggcccgcc atggggcaac tcgaacgaag gagcggctga tggatcttca
     1981 ggcacagatc gtcgaagcga tcaggctgga gctggcgcga cagtccgggg gcgccggcgc
     2041 ggcgctgaac ttcagcgacg acgagggacg ggccaaggtg aacggcagcc tcgaccttga
     2101 agcgctggcg atggccatct cgggcgccgt cgcaggcgga ccctgacgga cgggcggggc
     2161 ggtgagcgct ccgctcgccg acccccccgc ccgggtgatc cagttgaacc gacggtcagc
     2221 tcattcccaa ggacagtccc actgcgcctg ctcccagcgg aaaacgcagt tcgggagccg
     2281 aatatcgttc tcgtagatga actcctcggt gccgcagtag acctcgcccg aggcgtagag
     2341 gcagtagtcg ccgggcgcag cggccgccgg gcttacggtg agcaggccgg acgctgcaaa
     2401 cgtcgcggcc gcggcaaggg aacggatgga aatccgggca cgcatcttct tcctcccaac
     2461 agccccagtg gctgtaacgg catgagatga tcgattatgc cgccgatgca actcacggtc
     2521 gcgtgtagag gctcatgaaa cctgagcgtc gagccgcccc cgagccactc gtgactgcag
     2581 cgccatcaac cggaagacca cgctgtgtga aggcggtagg cgcggaggga ctcgaacccc
     2641 caaccagacc gttatgagcg gtcggctcta accattgagc tacgcgcccg ccgtgggcgt
     2701 ggcctagcag gcggggcggc ggcttgccaa gcgcctcggc cccgcctcca tcgggccttc
     2761 gacctgtcgg cgatttcacg ccgatgaacg gaaactgcca aaggtacgca ggcgttcatt
     2821 cagcggccaa cggtcaaggt gcggccatgg tcgtcgccca ccttcgggcg aacggcccaa
     2881 tgctttcagg agttccgagc ccatgaaccg tcccgctgtc gccgccgccg cgcccgccgt
     2941 ctccaaccgc ctgctgctga tgagcggcgc ggtgatggcc gccgccgccc tgttcgccgc
     3001 ccccgccgcc cgcgcccagt cccagccgga aggcggcgcg ccgatggtga ttcacgccgt
     3061 gg
//
LOCUS       DASVVN010000010         5280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_237161,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000010 DASVVN010000000
VERSION     DASVVN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237161"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VF686_00195"
     CDS             complement(<1..764)
                     /locus_tag="VF686_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8660253.1"
                     /translation="MRLAVAVLAGVILVAPGGAEARPLRVLSLDQCADQYALALAPDA
                     DLALSPRADDPDAWMRNAAVGRPRVRPTLEAAIAFQPDVVVRYWGGEPRLLARLQADG
                     VRVLTIEDATDFDGVRADIRTVSKGLDAAERGEALISRMNATLPPRAASEATTKPRAQ
                     YLTAGGFTAGKGTLIDAILSAAGFANAAEAPFFAPVSIERVVLFPPARFVLGFFDQAR
                     GDWRGAGRHPVLRRLMRGRVAARLPAAALTCPGWFAA"
     gene            complement(761..1390)
                     /locus_tag="VF686_00200"
     CDS             complement(761..1390)
                     /locus_tag="VF686_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8660254.1"
                     /translation="MTAVTLNPRSQRKPKGEGHARRAEILEAAERIFVEHGYEGATIR
                     KIADEVGLSSTALYMHFSEKSEILHEICSRAFSALLEANEGVTTRTGDPVARLRQMLE
                     AYVAFGFANPNAYRLAYMTRPIELREGAQSAARELGHELFRSFEKAVEDVEAAGLLRG
                     DARTTAQALWAGIHGVVSLMITKPYFDWVDRETLVTTLLDGLIAGLLKP"
     gene            1694..2962
                     /locus_tag="VF686_00205"
     CDS             1694..2962
                     /locus_tag="VF686_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8660255.1"
                     /translation="MTIVVWRLALIVLSAVSSPPPPEPVDWPRYTVVAALYQEAEVVG
                     QLIERLSRIDYPTDRLQGLLVLEAHDHETIAAARSARRPDWLEVFVVPPGTPQTKPRA
                     LNCALAHATGDLLTVYDAEDDPDALQLREAAARFAADTTGKLACLQAPLRIRRQGRAS
                     DPSPFLDRQFAAEYASLFEATLPGIARLGLPFPLGGTSNHFRTDVLSAVEGWDAWNVT
                     EDADLGFRLWSRGWKLGVISRPTWETPPGGLDNWLPQRTRWLKGYMQTWGVHTREPWR
                     LGWRGAAALILTVGAGLVSAALHGLSVAWVAATVLVALMAGLPPETSALALSVLVLGA
                     AAAWLSCAIGARRAGVPYGPGDMIAAPAYWALLSLAFVHAAWRLVLDPYAWDKTRHRR
                     DAPAETQDVLDETAPLRLSAVHAAAPEPVA"
     gene            2934..3860
                     /locus_tag="VF686_00210"
     CDS             2934..3860
                     /locus_tag="VF686_00210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8660256.1"
                     /translation="MPPRLNPSPETLVTRAWADYALLDSGGGRKLERYGRFTVVRPEP
                     QCFWSPRDPSAFDVADAVFDPTDEDESGRWRFAGNPIDTFPLAWRDVRFTSRFTPFRH
                     LAFFPEQAANWEWLDGRVRTLRSSSPKGGSATKNAPKVLNLFGYTAVASLACAAAGAE
                     VTHVDASKKSVAWARENAELSGMSDKPIRWIVEDCRKYVAREVRRGSKYDGIILDPPK
                     YGRGPTGEVWRLFEDLPGLLTDCAALLSDDASFLLLNAYAARISGLSLSHMMAEATAD
                     RGGTIDWGELALAEDGPDGRAIGLSFFARWSA"
     gene            3848..4669
                     /locus_tag="VF686_00215"
     CDS             3848..4669
                     /locus_tag="VF686_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8660257.1"
                     /translation="MVGVTAPSAQDRLITSLTNDTVKAVRALHMRKERETTGRFLAEG
                     LKFIGEALDQKRAPRLLLVGEDARPHPILDRAREATRAGGGEVVVVTHAILEKISRRD
                     NPQTVLGVFDQVYTPLSAILPASAPAWVALEQVRDPGNLGTIIRTADSAGCGGVILIG
                     DCVDPYSVEAVRATMGSVFAVAIARATPAEFLAWRQSWPGSVIGTRLDAAHGYRDAAI
                     QHPALILMGNEQAGLTDALAAACDVNVKIPMRGRADSLNLAVATGVMVYAVTDAA"
     gene            4753..>5280
                     /locus_tag="VF686_00220"
     CDS             4753..>5280
                     /locus_tag="VF686_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEX8660258.1"
                     /translation="MPIGLLLLFALACALPGAVAAQETEPDAAAYRQDALSIEPLINS
                     QYAYLDRLPGGVMPMSPVLRAEAEAVSDRRGLLRYAEHAVAALADHHAITGGSFADSW
                     GLVPSYSDLWIEPEGDAWRITAVRNGSPAALAGIEPGDALTSVEDLPTARAVEAFWSA
                     LGLQAEGDRAGYAARV"
BASE COUNT          753 a         1843 c         1860 g          824 t
ORIGIN      
        1 gccgcgaacc agccggggca tgtcagggcc gcggcgggaa gccgggcggc gacccggccg
       61 cgcatcagcc gccgcagcac cggatgacgg cccgccccgc gccagtcgcc gcgggcctga
      121 tcgaagaagc cgagcacgaa ccgcgccggc ggaaacagca ccacccgctc gatgctgacc
      181 ggcgcgaaga acggcgcctc ggccgcattg gcgaagcccg ccgccgacag gatggcgtcg
      241 atcagcgtcc ccttccccgc cgtgaagccg ccggccgtca gatactgggc ccgcggtttc
      301 gtagtcgcct cgctcgccgc gcgtgggggc agggtcgcgt tcatccggga gatcagggcc
      361 tcgccgcgct ccgccgcgtc cagccctttc gacaccgtcc ggatatcggc gcgcacgccg
      421 tcgaagtcgg tcgcgtcctc gatggtcaga acgcgtacgc cgtccgcctg cagccgagcc
      481 agcaggcgag gctcgccccc ccagtagcgg accaccacgt cgggctggaa ggctatggcc
      541 gcctccagcg tcggccggac tcgcgggcgt ccgacggcgg cgttccgcat ccatgcgtcc
      601 ggatcgtcgg cgcgcggcga cagggccagg tcggcgtcgg gggcgagggc cagcgcatac
      661 tggtcggcgc actgatccag cgacaggacc cgcagcggac gcgcctcggc cccgcccggc
      721 gcgaccagga tcacgccggc gaggaccgcg accgcgagcc tcatggcttc aggagacccg
      781 cgatcaaccc gtccagcagg gtcgtcacca gcgtctcgcg gtcgacccag tcgaaatagg
      841 gcttggtgat catcagcgac accaccccgt gaattcccgc ccagagggct tgggctgtgg
      901 tgcgcgcgtc gccgcgcagc agaccggccg cctcgacgtc ctcgacggcc ttttcgaacg
      961 accggaacag ttcatgaccc agttcgcgcg ccgccgactg cgccccttcg cgcagttcga
     1021 tggggcgggt catataggcc agccgatagg cgttgggatt ggcgaaaccg aaggcgacat
     1081 aggcctccag catctgccgc agtcgcgcca ccgggtcgcc ggtccgggtg gtcacgccct
     1141 cgttggcctc cagcagcgcg gagaaggcgc ggctgcagat ctcgtgcagg atctcgctct
     1201 tctccgagaa atgcatgtag agcgccgtcg acgacaggcc gacctcatcc gcgatcttgc
     1261 ggatggtggc gccctcatag ccgtgctcga cgaagattcg ctcggcggcc tcaaggattt
     1321 ccgcacggcg cgcgtggcct tcgcccttgg gctttctctg cgagcgggga ttgagggtta
     1381 cggcggtcaa acgcacactc cgaacgtcat ctcctgaata gcggcattcc cccgcccgcg
     1441 ccaaccgctt gccctgcgcg aaacggcacg ataaccatgc gttgtggaga ggttgtggat
     1501 aaacaatctg gaggatcgtc ggcgcgagac ccggcgatct ggaccgccgt tcgatcggag
     1561 cgcggcccgg cgcagtcttg cgcctgcgca gtgcgtggcg ctttgcggac tcgtcgttct
     1621 ggtcggagga tttgtctggt tcgagccccg actgttcgga catggccttg tcgccgcggg
     1681 cgcatggggt tttttgacga tcgtcgtctg gcggctcgcg ctgatcgtgc tcagtgcggt
     1741 gtcttccccg ccgccgcccg aacccgtcga ctggccgcgc tatacggtcg tggcggcgct
     1801 gtatcaggag gcagaggtcg tcggtcagct gattgagcgg ctgtcgcgga tcgactatcc
     1861 caccgaccgg ctgcagggcc tgctggtgct cgaggcgcac gatcacgaga ccatcgccgc
     1921 cgcccggagc gcccgccggc ctgactggct ggaggtgttc gtggtcccgc cgggcacgcc
     1981 acagaccaag ccgcgcgcct tgaactgcgc cctcgcccac gccacgggcg acctgctgac
     2041 ggtctatgac gccgaggacg accccgacgc gctgcagctt cgcgaggcgg cggcgcggtt
     2101 cgcggcggac acgaccggca agctggcctg cctgcaggcg ccgctgcgca ttcggcggca
     2161 aggacgggcg tcagacccct cgccctttct cgaccggcag ttcgcggcgg aatacgccag
     2221 cctgttcgag gccaccctgc ccggcatcgc ccgcctcggc ctgcccttcc cgctgggagg
     2281 caccagcaac catttccgca ccgacgtgct cagcgccgtc gaaggctggg acgcgtggaa
     2341 cgtcaccgag gacgcggatc tcggcttccg tctgtggagc cgaggctgga agctgggcgt
     2401 gatctcacgg ccgacatggg agacgccgcc gggtgggctc gacaactggc tgccgcagcg
     2461 aacgcggtgg ctgaagggtt acatgcagac ctggggggtg catacgcgtg agccgtggcg
     2521 gctcggctgg cgcggcgcgg cggcgttgat cctgaccgtc ggggcaggtc tggtgtcggc
     2581 ggcgctgcat gggctgtcgg tggcctgggt ggcggcgacc gtactggtgg cgctgatggc
     2641 gggcctcccg ccggagacct ctgccctcgc cctgagcgtg ctggtgctgg gcgcggcggc
     2701 ggcctggctg tcctgcgcca tcggggcgcg gcgggcgggc gtgccttacg gtccgggcga
     2761 catgatcgcc gctccggcct actgggccct gctcagtctc gccttcgtcc acgccgcgtg
     2821 gcgtctggtt ctcgatccct atgcctggga caagacgcgt catcgccgcg atgcgccggc
     2881 ggaaacgcaa gacgtgctgg acgagaccgc gcctctccgc ctatcagccg tgcatgccgc
     2941 cgcgcctgaa cccgtcgcct gagaccctgg tcacccgcgc ctgggccgat tacgccctgc
     3001 tggacagcgg cggcggccgc aagctggaac gctatgggcg cttcacggtc gtccggccgg
     3061 agccccagtg cttctggtcg ccgcgcgatc cgtcggcctt cgacgtcgcg gacgccgtgt
     3121 tcgacccgac cgacgaggac gagagcgggc gctggcggtt cgcgggaaac ccgatcgaca
     3181 ccttcccgct ggcgtggcgg gacgtgcgct tcaccagccg gttcacgcca ttccgccatc
     3241 tggccttctt ccccgaacag gccgcgaact gggaatggct ggacgggcgg gtgcggacac
     3301 tgcgctcaag tagcccgaag ggcggtagcg caacaaagaa tgcgccgaag gtgctgaacc
     3361 tgttcggcta cacggccgtc gccagcttgg cctgcgccgc ggctggcgcg gaggtgaccc
     3421 acgtcgacgc ctccaagaag tccgtggcct gggcacgcga gaacgccgag ctgtcgggca
     3481 tgtccgacaa gccgatccgc tggatcgtcg aggactgccg caaatacgtg gcgcgggaag
     3541 tacgacgcgg gtcgaaatac gacgggatca tcctcgaccc gcctaaatac gggcgcgggc
     3601 cgaccggcga ggtgtggcgg ctgttcgagg acctgccggg cctgttgacg gactgcgccg
     3661 ccctgctgtc cgacgacgcg tccttcctgc tgctgaacgc ctatgcggcg cgcatctcgg
     3721 gcctgtcgct gtcgcacatg atggccgagg cgaccgccga ccggggcggg accatcgact
     3781 ggggcgagct ggcgctggcc gaggacgggc cggacgggcg cgccatcggc ctgagcttct
     3841 tcgcgcgatg gtcggcgtga cggccccctc cgcccaggat cgactgatca cctccctgac
     3901 caacgacacg gtcaaggccg tgcgcgccct tcacatgcgc aaggaacggg agaccacggg
     3961 ccggtttctg gccgaagggc tgaaattcat cggcgaggct ctggaccaga agcgggcccc
     4021 tcgcctgctg ctggtcggcg aggacgcgcg gccgcatccg atcctggacc gcgcccgcga
     4081 ggcaacccgg gcgggcggcg gcgaggtggt ggtggtcacc cacgccatcc tcgagaagat
     4141 cagccgacgc gacaacccgc agacggtgct gggtgtgttc gatcaggtct atacgcccct
     4201 gtcggcgatc ttgcccgcgt ccgctccggc ctgggtggcg ctggagcagg tgcgcgatcc
     4261 gggcaatctg ggcaccatca tccgcacggc ggattccgcc ggctgcggcg gggtcatcct
     4321 gatcggggac tgtgtggacc cgtattcggt cgaggcggtg cgggcgacca tgggctcggt
     4381 cttcgccgtc gccatcgccc gggccacgcc ggccgaattt ctggcgtggc ggcagagctg
     4441 gccgggcagc gtcattggca cccggctgga cgcagcccac ggctaccgcg acgcggcgat
     4501 ccagcatccc gccctgatcc tgatgggcaa tgagcaggct gggctgaccg acgccctggc
     4561 cgccgcctgc gacgtcaacg tcaagattcc gatgcgcggc cgggccgaca gcctgaactt
     4621 ggccgtggcg accggcgtca tggtctatgc ggtgaccgac gcggcctgat ctcgcggaga
     4681 tggggcggga ttcacttgcc ggatcgcgat gcgcaggcga gcgtcgatcc accccgtcgc
     4741 caaggttcct cgatgcccat cggtctgctg ctgttgttcg cccttgcctg cgccctgccc
     4801 ggcgctgttg cggcgcagga aacggaacct gacgcggccg cctaccggca ggacgccctc
     4861 tccatcgaac ccctgatcaa cagccagtat gcctatctcg accggcttcc cggcggcgtc
     4921 atgcccatga gccccgtcct gcgagcagag gcggaggcgg tgtcggaccg gcgcggcctg
     4981 ctgcgctatg cggagcatgc cgtggccgct ctcgccgacc atcacgccat cacgggcggc
     5041 tcgttcgccg acagctgggg actggtgccc agctattcgg atctgtggat cgagcctgag
     5101 ggcgacgcgt ggcgcatcac ggcggttcgc aacggctctc cggccgccct tgccggcatt
     5161 gagccgggcg acgccctcac ctcggtggaa gatcttccga cggcccgggc ggtcgaagcg
     5221 ttctggtctg cgctggggtt gcaggctgag ggcgaccgcg ccggatacgc cgcccgtgtt
//
LOCUS       DASVVN010000011         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_500373,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000011 DASVVN010000000
VERSION     DASVVN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500373"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF686_00225"
     CDS             complement(<1..332)
                     /locus_tag="VF686_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX8660259.1"
                     /translation="MQASRRQFLMGAAALSAAVVTPETVRAMAAMQASADWALATADL
                     EADIAPRALRLVHGRAPAGLTGTLFRNGPAKFRRPGGSATHWFDGDGLMRAFRVSDGQ
                     ATLEARFA"
     gene            complement(343..759)
                     /locus_tag="VF686_00230"
     CDS             complement(343..759)
                     /locus_tag="VF686_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8660260.1"
                     /translation="MLHLALAAALVLTAAPAVAQDAGSSVVITFETGARTGSVMVALY
                     NSEASYGAGPAVAQAAISASGEVVARFENLPAGDYAVKAFHDVNGDGRMTTNPFGIPI
                     EPYAFSNNAVGNMGPARWAAARFPVSGETAQTIVIR"
     gene            complement(918..1799)
                     /locus_tag="VF686_00235"
     CDS             complement(918..1799)
                     /locus_tag="VF686_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX8660261.1"
                     /translation="MRPAAAFGPGAAMPDSAPASFPGFASADLAFLSQLAARNDRDWF
                     TANRAVYDDVLKPALGALIADASTACAARGLALGGDPNKSQFRIHRDVRFSKNKQPYK
                     THLSAVLTRDGLKMSPGMAYIHIEPEGGSRVTADSFDYDSIDPLDPSTHPGARLSVEP
                     EPAVEPLGIPDVEDAHGRGPYVSAGFFLYDRKNSERVRRAIVADPAAWLAIEKQLKAK
                     GLRLAPGLAVKRMPKGYEDHAGGPLEGALKRTQWYARRPLTGAEIASPDLPETLAGFV
                     ADMTPLLHFGWGALDKV"
     gene            complement(1805..2374)
                     /locus_tag="VF686_00240"
     CDS             complement(1805..2374)
                     /locus_tag="VF686_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660262.1"
                     /translation="MPDGSPTPPADALPAPFEFRRPLQNWRDDGHGRRTAPDEVWAAV
                     REDYLAGLSAYEVCERHGVRLTAMRNRAAREGWRRSDQPWAPRNRLDPEDEGVALEAR
                     IGGDLNKVELRELSYIASRRMMRAVLRGDAMDALRWRKVRDAMEAEEAEMERLGEQQD
                     AIRDARLGYAEVDSVDSTDASDGVFRPEG"
BASE COUNT          379 a          866 c         1106 g          401 t
ORIGIN      
        1 tcggcgaaac gagcctccag cgtcgcctgc ccgtccgaga cgcggaacgc ccgcatcagg
       61 ccgtcgccgt cgaaccagtg ggtggccgag ccgcccgggc ggcggaactt cgccgggccg
      121 ttgcggaaca gggttccggt caggccggcg ggagcgcggc cgtggacgag gcgaagggcg
      181 cgcggggcga tgtcggcctc gaggtcggcg gtggccagcg cccagtcggc ggaagcctgc
      241 atggcggcca tggcgcggac ggtttcgggc gtcacgacgg cggcggagag ggcggcggcg
      301 cccatgagga actggcggcg ggaggcttgc atcgcggaac ccctatcgga tgacgatggt
      361 ctgggcggtt tcgcccgaga cggggaagcg ggcggcggcc cagcgggcgg gacccatgtt
      421 gccgacggcg ttgttggaga aggcgtaggg ctcgatgggg atgccgaagg ggttggtggt
      481 catccggccg tcgccgttga cgtcgtggaa ggccttcacg gcgtagtcgc cggcgggcag
      541 attttcgaag cgggcgacga cctcgcccga ggcggagatg gcggcctggg cgacggcggg
      601 gccggctccg tacgaggcct ccgaattgta gagggcgacc atgaccgagc cggtgcgggc
      661 gccggtctcg aaggtgatga cgacgctgga gccggcgtcc tgcgccacgg ctggagcggc
      721 ggtcaggacg agggcggcgg cgagggccag atgcagcatg gggcgggtcc ttctgaaggg
      781 cccgacgacc gggccgtttc gatggactga ccgtgaggcg gctcccgggg gttcgccatg
      841 cggattgcgg gttcgggcgg gctccgttcg cgaacggcgg cgggtgttcg ttcacgtagc
      901 gcgggcgaac agcctcctca gaccttgtcc agcgcccccc atccgaaatg cagcagcggg
      961 gtcatgtcgg cgacgaagcc ggcgagggtt tcgggcaggt cggggctggc gatctcggcg
     1021 ccggtcaggg ggcggcgggc gtaccactgg gtgcgcttca gcgccccttc gagcgggccg
     1081 ccggcgtggt cctcatagcc cttgggcatg cgtttgacgg cgagcccggg ggcgagtcgc
     1141 aggcccttgg ccttcagctg tttctcgatg gccagccagg cggcggggtc ggcgacgatg
     1201 gcgcggcgga cgcgctcgct gttcttgcgg tcgtagagga agaagccggc cgagacatag
     1261 gggccgcggc cgtgggcgtc ctcgacgtcg gggatcccga gcggttcgac agcgggctcg
     1321 ggctcgacgg acagccgcgc acccggatgg gtcgagggat ccagcgggtc gatcgagtca
     1381 tagtcgaagc tgtcggcggt gacgcgcgac ccgccctcgg gctcgatatg gatataggcc
     1441 atgccgggcg acatcttcag cccgtcgcgg gtcaggacgg ccgacaggtg cgtcttgtac
     1501 ggctgtttgt tcttcgagaa gcggacgtcg cggtggatgc ggaactggct cttgttcggg
     1561 tcgccgccca gcgccagacc gcgcgcggcg caggcggtcg aggcgtcggc aatcagggcg
     1621 cccagcgccg gcttcagcac atcatcgtag acggcgcggt tggcggtgaa ccagtcgcga
     1681 tcgttgcggg cggcgagttg ggacaggaag gccaggtcgg cggaggcgaa gccggggaag
     1741 gaggcgggcg cggagtcggg catggccgca cccggaccga acgcggcggc ggggcgcaag
     1801 cctgtcaccc ctccggccga aaaacaccgt ccgaagcgtc cgtagagtcc actgagtcca
     1861 cttcggcata gcccagtcgg gcgtcccgga tcgcgtcctg ctgttccccc agacgctcca
     1921 tctcggcctc ctcggcctcc atggcgtcac gcaccttgcg ccaacgcagg gcgtccatgg
     1981 cgtcgccgcg caggaccgcc cgcatcatgc gccggctcgc gatatacgag agctcgcgca
     2041 gctcgacctt gttcaggtcg ccgccaatgc gggcctccag cgcgacgccc tcgtcctcgg
     2101 ggtcgaggcg gttgcggggc gcccatggct ggtcgctgcg gcgccagccc tcgcgcgcgg
     2161 cgcggttgcg catggcggtg agccggacgc cgtggcgctc gcaaacctcg tacgcggaca
     2221 ggccggccag atagtcctcg cgcacggcgg cccagacctc atcgggggcg gtgcggcggc
     2281 cgtggccatc gtcgcgccag ttctggagcg ggcggcggaa ttcgaacggc gccgggaggg
     2341 cgtctgcggg cggggtgggc gagccgtcgg gcatggtggg ttctccgtcg tgaaacgggg
     2401 aatcatacgg gcggttcggg gcgtgcgagg ctggtggggg tgcggcggcc cgcaagcggc
     2461 tggattcgct gggcgcgggc ggttttcagg gggtgcagga aaaagttcgc actcgccgtc
     2521 tcctccctgt cgcgaagcga tggggaggtg gcgcggcgcg ccttcgcacc gtgacggtgg
     2581 ggtagatgca gtgcgcggac ccggcgcccc tccaccgctt cgcggtcccc ctccccatcg
     2641 cttcgcgaca gggaggagac cgaaggcgcg ccctacctga acacgcagtt cacgccggcc
     2701 cacatcgagg tgatggtggc ggcgtcggtc gttccggaca ggaagccgta ga
//
LOCUS       DASVVN010000012         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_79502, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000012 DASVVN010000000
VERSION     DASVVN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79502"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /gene="nuoL"
                     /locus_tag="VF686_00245"
     CDS             complement(<1..1529)
                     /gene="nuoL"
                     /locus_tag="VF686_00245"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX8660263.1"
                     /translation="MNLETLVTLGVFAPLLGAAIVGLSGRRLGDLISKSITTGLLFFS
                     CAVAWTVFSQWTWGGLQAFTVELFPFINVGDFQSVWSIRVDALSATMLVVVTTVSSLV
                     HLYSWGYMSEDPDKPRFFAYLSLFTFAMLALVTAADFMQLFFGWEGVGLASYLLIGFW
                     YKKSTASAAAIKAFVVNRVGDFGFALGIFTVFWMFGTIQFAELFPMISSKAGTGWEFL
                     GHTWSALDLAAFLLFIGAMGKSAQFFLHTWLPDAMEGPTPVSALIHAATMVTAGVYMV
                     CLLSPIFEYAPVASAIVALVGAVTALFAATVGLAQNDIKRVIAYSTCSQLGYMFFAAG
                     VGAYQAAMFHLFTHAFFKALLFLGAGSVIHGMHHEQDMRKMGGLWRLLPVTYAVMTIG
                     TIAITGLGIPGVGGFAGFYSKDSIIESAFASFANGHSAMGLFAFMVGILAAGLTAYYS
                     WRLVFMTFHNKPVWKDAHHDDHAHGHDDHASHAQLETHSEPIADDHHADDGHAHDDHG
                     H"
     gene            complement(1532..1840)
                     /gene="nuoK"
                     /locus_tag="VF686_00250"
     CDS             complement(1532..1840)
                     /gene="nuoK"
                     /locus_tag="VF686_00250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX8660264.1"
                     /translation="MAVTLQHYLAVAAILFTIGVFGIFVNRKNVIIILMSIELILLAV
                     NINFVAFSAYLNDVQGQIMAMFVLTVAAAEAAVGLAILVTFFRNRGDIAVDDASVMKG
                     "
     gene            complement(1840..2469)
                     /locus_tag="VF686_00255"
     CDS             complement(1840..2469)
                     /locus_tag="VF686_00255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX8660265.1"
                     /translation="MLLQGLAFYLLSAVAVLGGLMVVTAKNPVHSVLWLILAFFSSAG
                     LFVLLGAEFLAMLLVVVYVGAVAVLFLFVVMMLDVDFVKLREGYARYLPLAMIVAGIL
                     LAEMIIISLTVVSGGAAADAIGPVAATAEMSNVEAIGRVLYTDYVYIFQAAGIVLLIA
                     MIGAIVLTLRHKPNIKRQKPHDQVTRDRKTAVEVKGAVTGAGVTPEDLV"
     gene            2705..3520
                     /locus_tag="VF686_00260"
     CDS             2705..3520
                     /locus_tag="VF686_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8660266.1"
                     /translation="MSEEQDYLLGTHDAEVERLGLQHRVWRPRVLDAWRRAGITVGST
                     VVDAGAGPGWASLDLAEIVGPTGRVHALERSGRFVDVLNRSAAARGLGNIEAATVDLV
                     TDPLPASGADAFWIRWVLAFVSDPAAVLKKLHATLRPGGVAVIHEYLDYGTFSILPDN
                     PAVEDFKRLVVEDWRASGGEPDIGRALPAMLPAAGFRIRSMQPIVETARKNDLVWQWP
                     ATWARNYPRFLLESGKVDQAWYDRVIAAFDAADANPDAVMITPMVVEIIAEKV"
     gene            complement(3560..4051)
                     /gene="nuoI"
                     /locus_tag="VF686_00265"
     CDS             complement(3560..4051)
                     /gene="nuoI"
                     /locus_tag="VF686_00265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX8660267.1"
                     /translation="MFTRIVQATKGAMMLDVIGAVGLSLKYMMRPKATVNYPFERNPQ
                     SPRFRGEHALRRYANGEERCIACKLCEAICPAQAITIESEPRADGSRRTTRYDLDMVK
                     CIYCGLCQEACPVDAIVEGPNLEFTTETREELLYDKDRLLANGDRWERLIAKNLELDA
                     PYR"
     gene            complement(4051..4266)
                     /locus_tag="VF686_00270"
     CDS             complement(4051..4266)
                     /locus_tag="VF686_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660268.1"
                     /translation="MIRTLTLVIAAATLSACTIPTYEADPVSVYQWERRQERIQREEA
                     ERLRRCETMDKQSERYARECVRPGASQ"
BASE COUNT          804 a         1448 c         1476 g          684 t
ORIGIN      
        1 tggtggccgt ggtcgtcgtg cgcgtgcccg tcgtcggcat gatgatcgtc ggcgatcggt
       61 tccgaatggg tttccagctg ggcgtgcgag gcgtggtcgt catgcccgtg cgcatgatcg
      121 tcgtgatgcg cgtccttcca caccggcttg ttgtggaagg tcatgaacac cagccgccac
      181 gaatagtagg cagtcaggcc cgcggccagg atgccaacca tgaaggcgaa cagacccatg
      241 gcgctgtggc cgttggcgaa cgaggcgaag gcgctctcga tgatcgagtc cttggaatag
      301 aagcccgcga agccgccgac gccgggaatg cccaggccgg tgatggcgat ggtgccgatg
      361 gtcatcaccg cataggtgac cggcagcagc cgccacagcc cgcccatctt ccgcatgtcc
      421 tgctcgtggt gcatcccgtg gatgaccgag ccggccccga ggaacagaag ggccttgaag
      481 aaggcgtggg tgaacaggtg gaacatggcg gcctgatagg cgccgacgcc cgccgcgaag
      541 aacatgtagc ccagctgcga acaggtcgaa taggcgatga cccgcttgat gtcgttctgg
      601 gccagaccca cggtggcggc gaacagggcc gtgaccgcgc cgaccagcgc cacgatagcc
      661 gaggccaccg gcgcatattc gaagatcggg ctcagcaggc agaccatata gacaccggcg
      721 gtgaccatgg tcgccgcgtg tatcagggcc gacaccgggg tcgggccctc catggcgtcc
      781 ggcagccagg tgtgcaggaa gaactgggcc gacttgccca tggccccgat gaacagcagg
      841 aaggccgcca gatcgagcgc cgaccaggtg tggccgagga attcccagcc ggtgcccgcc
      901 ttcgaggaga tcatcgggaa cagttcggcg aactggatcg tgccgaacat ccagaagacg
      961 gtgaagatgc ccagggcgaa gccgaagtcg ccgacccggt tgaccacgaa ggccttgatg
     1021 gcggcggcgc tggccgtgga cttcttgtac cagaaaccga tcagcagata ggacgccagc
     1081 cccacccctt cccagccgaa gaacagctgc atgaagtcgg cggcggtgac cagcgccagc
     1141 atggcgaagg tgaacagcga cagataggcg aagaagcgcg gcttgtcggg gtcctccgac
     1201 atatagcccc agctgtacag gtgcacgagc gacgacaccg tcgtcaccac gaccagcatg
     1261 gtggccgaca gggcgtcgac ccggatcgac cacaccgact ggaagtcgcc gacgttgatg
     1321 aaggggaaca gctcgaccgt gaaggcctga aggccgcccc aggtccactg gctgaacacg
     1381 gtccaggcga ccgcgcacga gaaaaacagc agtccggtcg tgatggattt ggaaatcagg
     1441 tcgccgagcc ggcggccgga caggccgacg atggccgcgc cgagcagcgg ggcgaagacg
     1501 ccgagagtga cgagggtctc gagattcacg gtcagccctt catcacgctg gcgtcgtcaa
     1561 ccgcgatgtc gccgcggttg cggaagaagg tcaccagaat ggccagaccc acggccgcct
     1621 cggcggccgc cacggtcagg acgaacatgg ccatgatctg cccctgcacg tcgttgagat
     1681 aggccgagaa ggcgacgaaa ttgatgttca cggccagcag gatcagctcg atcgacatca
     1741 ggatgatgat gacgttcttg cggttcacga agatgccgaa cacgccgatg gtgaacagga
     1801 tggcggcgac cgccagatag tgctgcaggg tgacagccat cagaccagat cctcgggagt
     1861 gacgccggcg ccggtgaccg cgcccttgac ctcgacggcc gtcttgcggt cgcgggtcac
     1921 ctggtcgtgc ggcttctggc gcttgatgtt tggcttgtgg cgcagggtca ggacgatggc
     1981 cccgatcatg gcgatcagca gtacgatccc ggccgcctgg aagatgtaga cataatcggt
     2041 gtacagaacg cgcccgatcg cctcgacatt ggacatctcg gccgtcgccg cgactggccc
     2101 gatcgcgtcc gcggcggccc cgccgctgac cacggtcagg gagatgatga tcatctcggc
     2161 cagcaggatg ccggccacga tcatcgccag cggcagatag cgggcatagc cctcgcgcag
     2221 cttaacgaag tcgacgtcca gcatcatgac gacgaacagg aacagcaccg ccaccgcgcc
     2281 gacgtagacg acgaccagca gcatggccag gaactccgcc ccgagcagga cgaacagccc
     2341 cgccgacgag aagaaggcca ggatcagcca cagcacgctg tgtacggggt tcttcgccgt
     2401 cacgaccatc aggccgccca gcacggccac agccgacaga agatagaagg ccaggccttg
     2461 caagagcatc gcgcgatcgg ctcctcacgc tttgccggcg agcgaggcgg cggccagcgg
     2521 atggttcaga caaaaacccg aaacccggcg cgcgaatccc gcccggaaag cctcggaaag
     2581 cgcgcgactt ctaaccgccg cgcggggctt tgcaaagacc gaatgcgacg accggtgacc
     2641 gcgtcttgcc gtcttgccgg agccggtccg cgaccccagt ttggtcggaa ccaaggggac
     2701 ggccatgagc gaagagcagg actaccttct gggcacccat gacgccgagg tcgaacgcct
     2761 cggcctgcag caccgcgtct ggcggccgcg cgtgctcgac gcatggcgcc gggccgggat
     2821 taccgtcggt tcgaccgtcg tcgacgccgg cgccggaccc ggttgggcca gtctggatct
     2881 ggccgagatc gtcggcccca ccggccgtgt gcacgccttg gaacggtcgg gccgtttcgt
     2941 cgatgtgctg aatcggtcgg ccgccgcgcg cggcctcggc aatatcgaag ccgccacggt
     3001 cgatctggtc accgatcccc tgcccgcctc gggcgccgac gccttctgga ttcgctgggt
     3061 cctggccttc gtcagcgacc ccgccgcggt gctgaaaaag ctgcacgcca cgcttcgccc
     3121 cggcggcgtc gcggtgatcc acgagtatct ggactacggg accttctcga tcctgcccga
     3181 caatccggcg gtcgaggact tcaaacggct ggtcgtcgag gactggcgcg cctcgggcgg
     3241 cgagcccgac atcggccgcg ccctgcccgc catgctgccc gccgccggct tccgcatccg
     3301 gtcgatgcag cccatcgtcg agaccgcgcg gaagaacgat ctggtctggc aatggccagc
     3361 cacctgggcc cgcaactatc cgcgcttcct gctcgagtcg ggcaaggtcg atcaggcctg
     3421 gtacgaccgt gtcatcgccg ccttcgacgc cgccgacgcc aatcccgacg cggtcatgat
     3481 caccccgatg gtcgtggaga tcatcgccga gaaggtctga ccttgtctcc tccccgctgc
     3541 ggagcaggca cgagacagat caccgatacg gcgcgtcgag ctcgaggttc ttggcgatca
     3601 gccgttccca gcggtcgccg ttcgcgagca gacggtcctt gtcgtacagc agttcctcgc
     3661 gggtctcggt cgtgaactcg agattcggcc cctcgacgat ggcgtcgacc gggcaggcct
     3721 cctggcacag gccgcagtag atgcatttga ccatgtcgag gtcgtagcgg gtcgtgcggc
     3781 ggctgccgtc ggcgcgcggc tcggactcga tggtgatggc ctgggcgggg cagatcgcct
     3841 cgcacagctt gcaggcgatg cagcgttcct cgccattggc atagcggcgc agggcgtgct
     3901 cgccccggaa gcgcggcgac tgcggattgc gctcgaacgg atagttcacc gtcgccttcg
     3961 gccgcatcat gtatttcagc gacaggccga ccgcgccgat gacgtccagc atcatcgcgc
     4021 ccttggtggc ctgaacgata cgggtgaaca ttactggctg gctccgggtc gtacgcactc
     4081 gcgggcgtag cgttcgctct gtttgtccat ggtctcgcag cggcgcaggc gctcggcctc
     4141 ctcgcgctga atgcgttcct gacgccgctc ccactgatag accgacacgg gatcggcctc
     4201 gtaggtcggt atggtgcagg ccgacagggt ggcggcagcg atgacgaggg tcagggtgcg
     4261 gatcatcggt ccctcctgac cacggtcttg cagccttccg gctggtcgtc gcggtcggac
     4321 cgctcgtcgc ggcattccgg atgcatgcgc gccgcctcgg cgcgcgcgcg ctcggcccgc
     4381 tccagcgaac ccgcgcccgt cgtctcctcc gc
//
LOCUS       DASVVN010000013         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_395183,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000013 DASVVN010000000
VERSION     DASVVN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395183"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF686_00275"
     CDS             <1..737
                     /locus_tag="VF686_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660269.1"
                     /translation="KPVMVGGMVLFLIAVFPGFHALTNAANPALAQAQASAPVTVIAD
                     PATCALQFDPIGKAAFASSCDIAKSVLSNAGVSYGNAEAIAGSPATVRIGAAEVASVE
                     GAGMAPAELKAARADVETRIKAALVEAGYPAKADPARINMPLVFGILMIFMVAATALY
                     GPQAAALVELFPTRVRYTAMSLPYNIGTGWVGGLLPAASFALVAASGNIYFGLWYSVA
                     FTVVAVIVSLLWLPETKGRDLDTIGR"
     gene            complement(741..1241)
                     /locus_tag="VF686_00280"
     CDS             complement(741..1241)
                     /locus_tag="VF686_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660270.1"
                     /translation="MEFWKTTAPWLLLAAGSGVMTLSGLAAHSAPFIPAVLCVIAAAC
                     AVHAPTRRRVMDQLLRAFSGSAVRSVLIIAGAMMLIQLLPLEMALLLAGDVLVYLEAV
                     MAISLLAAGTRLKPMRAVIVARATGVITRLTARRRDAVRAIRMSRPGGARRPPSDDSD
                     RARAFA"
     gene            complement(1303..1737)
                     /locus_tag="VF686_00285"
     CDS             complement(1303..1737)
                     /locus_tag="VF686_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8660271.1"
                     /translation="METARIERRIGVRATPEHIWGLIADLSYWDRWNPIEKGVEGTIA
                     FGGGITLTETIEGLPERRVSARVGEWEPYSQLVWTENRGLWFRSTRYFEIQRLDEPNA
                     CIVSNGFIFSGLRGEMFFDKHRAHLRHAVDAVAEAMKAAAEA"
     gene            complement(1781..>2948)
                     /locus_tag="VF686_00290"
     CDS             complement(1781..>2948)
                     /locus_tag="VF686_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660272.1"
                     /translation="AFAISALMWTIASPFWARLSDTLGRKRVILIGLSGFVVSMTGFG
                     LAATSGLEHWLGAGVAFALMAVARTVYGVFGSAAPIASQAYVADRTTPEQRTSAMSLL
                     ASAQGLGTVIGPALAPFFVLPLVGLAGPMYAFALFGAVVLLIVWRRLPSGEVVRVPSP
                     SGQRDPVAKGLWRDPRVRDYLLYGLLVTGAQAVNISVLGFHIIDELAATGVAARAAQP
                     FIGIAMLAGAAATLMAQWGLIPLLKLQPRDLMRWGAGLALLGNLMSIAAPGYYGVVVG
                     YAVVSMGVGFARPGFTAGSSLSVAPHEQGAIAGLMMSLAGLSFLAPPVIGVALYELAE
                     PGPFIANAVLLAAATALCFVSARLRAGPPDLPDRDETPSSDTPPASQPGPEGNR"
BASE COUNT          477 a         1026 c         1061 g          384 t
ORIGIN      
        1 gcaagcccgt gatggtgggc ggcatggtgc tgttcctcat cgccgtcttc ccgggcttcc
       61 acgccctgac caacgccgcc aatccggcct tggcccaggc ccaggcctcg gcccctgtca
      121 ccgtgatcgc cgaccccgcc acctgcgccc tgcagttcga cccgatcggc aaggccgcct
      181 tcgccagttc ctgcgacatc gccaagagcg tgctgtcgaa cgcgggcgtg tcctacggca
      241 acgccgaggc gatcgccggt tcgccggcga cggtccgcat cggcgccgcc gaggtggcct
      301 cggtcgaggg cgcgggcatg gcgccggcgg agctgaaggc ggcgcgcgcc gacgtcgaga
      361 cccgcatcaa ggcggcgctg gtcgaggccg gctatccggc caaggccgat ccggcgcgga
      421 tcaacatgcc gctggtcttc ggcatcctga tgatcttcat ggtcgcggcc acggccctgt
      481 acggaccgca ggcggcggca ctggtcgagc tgttcccgac gcgggtgcgc tatacggcga
      541 tgagcctgcc ctacaacatc ggcacgggct gggtcggagg gctgctgccc gcggccagct
      601 tcgccctggt ggcggcgtcg gggaacatct atttcggtct ctggtactcg gtggccttca
      661 ccgtggtggc ggtgatcgtc agcctgctgt ggctgcctga aaccaaggga cgggacctgg
      721 atacgatcgg gcgctgaccg tcaggcgaag gcccgggctc ggtcggaatc gtcagacggg
      781 ggacgccggg ctccgccggg ccgcgacatc cggatggccc ggacggcgtc gcggcgccgc
      841 gccgtcagcc gcgtgatgac ccccgttgcg cgggcgacga tcacagcgcg catcggcttg
      901 agccgggttc cggcggccag caggctgatc gccatgacgg cttccagata gaccagcacg
      961 tccccggcca gcagcagggc catctccagc ggcagcagct ggatcagcat catggctccg
     1021 gcgatgatca ggacggaccg cacagctgaa ccggagaagg cccggagcag ctggtccatc
     1081 acccgccgcc gcgtcggggc atggacggcg caggccgcgg cgatgacgca gagcacggcg
     1141 ggaatgaagg gcgcgctgtg cgccgccaga ccggacaggg tcatcacgcc cgatcccgcc
     1201 gcgagcagca gccacggagc ggtcgtcttc cagaattcca ttcaaatccc ctcagcgtcg
     1261 ccgttcctcg cagatggcgg cgcgccgggg cggcggcaag ggtcaggcct cggcggcggc
     1321 cttcatggcc tcggccacgg cgtcgacggc gtggcgcaga tgggcgcggt gcttgtcgaa
     1381 gaacatctcg ccgcgcaggc ccgagaagat gaagccgttg gacacgatac aggcgttggg
     1441 ctcgtccagc cgctggatct cgaaatagcg cgtcgagcgg aaccacaggc cgcggttctc
     1501 ggtccagacc agctgggaat agggctccca ctcgccgacc cgggccgaga cccgacgctc
     1561 tggcaggccc tcgatcgttt ccgtcagggt gatgccgccc ccaaaggcga tggtcccctc
     1621 cacgcctttt tcgatcgggt tccagcggtc ccagtatgac aggtcggcga tcaggcccca
     1681 gatgtgctcg ggcgtggcgc gcacgccgat gcggcgttcg atacgggcgg tttccatggc
     1741 tccgatctgc cacggctttc agacggagaa aaggccgcgg tcagcggttc ccttccggac
     1801 ccggctgcga ggcggggggc gtgtcggaag acggggtctc gtcgcggtcg ggcaggtcgg
     1861 gcggtccggc gcgcagccgg gcgctgacga agcacagggc cgtggcggcg gccagaagga
     1921 cggcgttggc gatgaagggg ccgggctcgg ccagttcgta cagggccacg ccgatcaccg
     1981 gcggggcgag gaagctgagg ccggccaggg acatcatcaa tccggcgatc gcgccctgct
     2041 cgtgcggcgc caccgacagg gacgagccgg cggtgaagcc cggtcgggcg aaaccgacgc
     2101 ccatcgacac cacggcgtag ccgaccacca cgccgtagta gcccggagcg gcgatgctca
     2161 tcagattgcc cagcaacgcc agaccggcgc cccagcgcat caggtcgcgc ggctgcagct
     2221 tgagcagcgg gatcagtccc cactgggcca tcagggtggc ggcggccccg gccagcatgg
     2281 cgatcccgat gaagggctgg gcggcgcgcg cggcgacgcc cgtcgcggcc agctcgtcga
     2341 tgatgtggaa gcccagcacc gagatgttga ccgcctgcgc ccccgtgacc agcaggccgt
     2401 acagcagata gtcgcgcacg cgcggatccc gccacagccc cttggccacc ggatcgcgct
     2461 ggccgctggg cgaggggacg cgcaccacct cgccgctggg cagccggcgc cagacgatca
     2521 gcaggacgac cgcgccgaaa agggcgaagg cgtacatcgg ccccgccagc cccacgagcg
     2581 gcaggacgaa gaagggcgcc agcgcggggc cgatcaccgt gcccagccct tgggccgagg
     2641 ccagcagcga catggccgag gtgcgctgtt cgggcgtcgt ccggtccgcc acataggcct
     2701 gcgacgcgat cggcgcggcc gaaccgaaca cgccatagac ggtgcgcgcc accgccatca
     2761 gcgcgaaggc cacgcccgca ccaagccagt gctccagccc ggaagtcgcg gcgaggccga
     2821 agccggtcat ggataccacg aagccggaca ggccgatcag gatcacccgc tttcgtccca
     2881 gggtgtcgga cagccgcgcc cagaacggcg aggcgatggt ccacatcagg gccgagatgg
     2941 cgaaggcg
//
LOCUS       DASVVN010000014         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_421389,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000014 DASVVN010000000
VERSION     DASVVN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421389"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VF686_00295"
     CDS             <1..698
                     /locus_tag="VF686_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX8660273.1"
                     /translation="RLQSNRWADAIDYFQEVERQHPYSEWSRRAILMQIYAYYQNNNY
                     AEAIAASDRFIQLFPGNPSAPYAFYMKALCNFEQITDVGRDQGYAQAALEGLKDVQRR
                     YPGTSYAIDATVKIDMVNDQLAGKEMAIGRYYQRAGQPLAAINRYKAVIDNDAYQRTS
                     HAPEALYRLVEVNLTLGLKEEATRNGAVLGFNYPGSPWYAEAYALLTEQGQQPGVTPT
                     GERESWLRRIIPG"
     gene            862..>1528
                     /locus_tag="VF686_00300"
     CDS             862..>1528
                     /locus_tag="VF686_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8660274.1"
                     /translation="MLTALSIRDIVLVDALDLEVEGGLTVLTGETGAGKSIILDALGL
                     ALGARADAGLVRAGAKQASATAVFTAPDDPALLEMIAERGFEVAPGEELILRRTVAAD
                     GRSRAFVNDQPAGVTALREIGQALVEVHGQHETVGLLDWKTHRALLDAYGGLQPQLGG
                     VAAAADRLKSAERAVADLKAAAADAASRAEELTLNLADLDALDPRADEETELAGERAL
                     LGAA"
BASE COUNT          258 a          549 c          499 g          222 t
ORIGIN      
        1 agcgtctgca gagcaaccgc tgggccgacg ccatcgacta tttccaggag gtcgagcgtc
       61 agcatccgta ttcggagtgg tcgcgccgcg cgatcctgat gcagatctac gcctactatc
      121 agaacaacaa ctacgccgag gccatcgccg cctcggaccg gttcatccag ctgttccccg
      181 gcaatccgtc cgcgccctac gccttctaca tgaaggccct gtgcaatttc gaacagatca
      241 ccgacgtggg ccgcgaccag ggctatgccc aggcggcgct tgaggggctg aaggacgttc
      301 agcgccggta ccccggcacc tcctacgcca tcgacgccac ggtcaagatc gacatggtca
      361 acgaccagct ggcgggcaag gaaatggcca tcggccgcta ctaccagcgc gccggccagc
      421 cgctggcggc gatcaaccgc tacaaggccg tgatcgacaa cgacgcgtat cagcgcacat
      481 cgcacgcgcc ggaagccctc taccgtctgg tcgaggtcaa tctgacgctg ggcctgaagg
      541 aagaggcgac ccgcaacggc gccgtgctcg gcttcaacta tccgggcagc ccctggtacg
      601 ccgaggccta cgccctgctg accgaacagg gccagcagcc cggcgtcacc ccgaccggcg
      661 agcgtgaaag ctggctgcgc cggatcatcc cgggctgatc ttcaacgacc cgtcatcctc
      721 ggacttgtcc cgaggaccca ttggtcagca ttctccggcg tcaccggtga aacgattcgc
      781 tttgtcctac gcccaacctt cctgccccgg acaaatgggc cctcgggaca agcccgaggg
      841 tgacggctgg gtagtggcta catgctgacc gccctctcca ttcgcgacat cgtgctggtg
      901 gacgcgcttg atctcgaggt cgagggcggc ctgaccgtgc tgaccggcga gaccggggcc
      961 ggcaagtcga tcattctgga cgccctcggt ctggcgctgg gagcccgcgc cgacgccggt
     1021 ctggtgcggg cgggggccaa acaggcctcg gccaccgccg tcttcacggc ccccgacgac
     1081 cccgccctgc tggagatgat cgccgagcgc ggcttcgagg tcgcgccggg cgaggagctg
     1141 atcctgcgcc gcaccgtcgc cgccgatggc cgcagccgcg ccttcgtcaa cgaccagccc
     1201 gcgggcgtca cggcgctgcg ggagatcggt caggcgctcg tcgaagttca tggtcagcac
     1261 gagacggtcg gtcttctcga ttggaagacc catcgcgccc tgctggacgc ctacggcgga
     1321 ctgcagccgc aactcggcgg cgtcgccgcg gccgcggacc ggctgaagag cgccgagcgc
     1381 gccgtcgccg acctgaaggc cgccgccgcg gacgcggcct cgcgcgccga ggaactgacg
     1441 ctgaacctgg cggacctcga cgcgctcgac ccgcgcgccg acgaggagac ggaactggcg
     1501 ggcgaacgcg ccctgctcgg cgcggcgg
//
LOCUS       DASVVN010000015         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_395214,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000015 DASVVN010000000
VERSION     DASVVN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395214"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VF686_00305"
     CDS             <1..115
                     /locus_tag="VF686_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX8660275.1"
                     /translation="FGGAVVRDAPLAPLAPARRKALTIAFARLGAMRMLRD"
     gene            complement(112..582)
                     /locus_tag="VF686_00310"
     CDS             complement(112..582)
                     /locus_tag="VF686_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660276.1"
                     /translation="MRLTALAVSALVLSACSQAEEPAPPPPAPEPAPVLAGVDLTRPV
                     RALGTEPFWSVELTGTEMVYTTPEPPEQRAPQPRPVVQGTTAMFEGKTAAGTALSVTL
                     VATECSDGMSDRTYPLTAIVKVGETSLNGCAASSAAIMSTGESGPVIEKAQPPA"
     gene            complement(647..>1623)
                     /locus_tag="VF686_00315"
     CDS             complement(647..>1623)
                     /locus_tag="VF686_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX8660277.1"
                     /translation="RISGVIRVYRAGCSACETSVAEPADWVMPPDAIWIDLVEPTREE
                     DAAVERALGLSVPTREEMAELEASSRLYRENGATYVTADIIHHGDQDTPSVDPVTFIL
                     TSGPLVTIRYFDPKPFTMLMDKLDRDPALCGTGADLFLNLMEAIIDRASDVLSTNAAK
                     VEAVSNHVFSGRKTVGFDHLITKLGRAHIANARIEQSLAGLSRIFAFAALDDRIEQHG
                     EPREHIKSLARDAQSLISHNQAVAASINFQLSAALGLINIEQSSIIKIFSVAAVAFLP
                     PTLIASIYGMNFDVMPELSQPWGYPAAVVAMVISAILPLAWFKKKGWL"
BASE COUNT          253 a          541 c          569 g          260 t
ORIGIN      
        1 tttcgggggg gcggtggtgc gcgatgcgcc gctggccccg ctggcgcccg cacgccgcaa
       61 ggccctgacc atcgccttcg cccggctggg cgccatgcgg atgctgcggg attaagccgg
      121 cggctgggcc ttttcgatca ccggcccgct ctcgcccgtc gacatgatcg ccgccgacga
      181 cgccgcgcag ccgttcaggc tggtctcgcc gaccttgact atggccgtca gcggataggt
      241 gcggtcgctc atcccgtcgg agcattcggt ggccacaagg gtgacgctga gcgccgttcc
      301 ggcggccgtc ttcccttcga acatcgcggt cgtgccctgg acgacggggc gaggctgcgg
      361 cgcgcgctgt tcgggcggct cgggggtcgt atagaccatc tccgttccgg tgagctcgac
      421 gctccagaac ggctcggtgc ccagcgcccg cacgggcctg gtcaggtcga ccccggccag
      481 caccggcgcg ggctcgggcg ccggcggcgg gggcgcgggc tcttcagcct gcgaacaggc
      541 cgacaggacc agggcggata cggcgagggc ggtcagacgc atggcgaggc tccgggggtg
      601 tcccgcacaa caaacgcgga cggggcccat cgggtcaacc ccgcgcctag agccagccct
      661 tcttcttgaa ccaggccaac ggcaggatgg ccgagatcac catggcgacc accgccgccg
      721 gatagcccca aggctgcgac agctcgggca tgacgtcgaa attcatcccg tagatcgagg
      781 cgatcagggt cggcggcagg aaggccaccg ccgccaccga gaagatcttg atgatcgacg
      841 actgctcgat attgatcagg cccagcgcgg ccgacagctg gaaattgatc gacgccgcga
      901 ccgcctgatt gtgcgaaatc aggctctggg cgtcgcgggc cagcgacttg atgtgttcac
      961 gcggttctcc gtgctgctcg atgcggtcgt ccagggcggc gaaggcgaat atccgcgaca
     1021 ggcccgccag actctgctcg atccgggcat tggcgatatg ggcccggccc agtttggtga
     1081 tcagatggtc gaagccgacc gtcttcctgc cgctgaagac atggttcgac accgcctcga
     1141 ccttggccgc attcgtcgac agcacgtccg aggcgcggtc tatgatcgcc tccatcaggt
     1201 tcaggaacag gtccgcgccc gtgccgcaca gggccgggtc gcgatccagc ttgtccatca
     1261 gcatggtgaa cggcttggga tcgaaatagc ggatcgtgac cagcggtccg gaggtcagaa
     1321 tgaaggtgac cggatccacg ctgggcgtgt cctggtcgcc gtggtggatg atgtcggcgg
     1381 tgacataggt ggccccattc tcgcggtaga ggcggctgga cgcctccagc tcggccatct
     1441 cctcgcgcgt cgggaccgac aggcccaggg cgcgctcgac ggcggcgtcc tcttcccggg
     1501 tcggctcgac caggtcaatc cagatggcgt cgggcggcat gacccagtcc gccggctccg
     1561 ccaccgaggt ttcgcaggcg ctgcagccgg cgcgatagac gcggatcacg ccggaaatcc
     1621 ggg
//
LOCUS       DASVVN010000016         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_132120,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000016 DASVVN010000000
VERSION     DASVVN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132120"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VF686_00320"
     CDS             complement(<1..1079)
                     /locus_tag="VF686_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8660278.1"
                     /translation="MPLASLLLAWRSEAATVTRRAPPIASILWLGPNIMLLKRKYLFS
                     STILAGVMAVAAPAFAQSQLPAVNVQGQADPEATEIDEVVVTGSRIRRDPVNAPTPLI
                     TVNREQLLETGQSTVIDYLATIPALSNSVVPSDTTGSNLGDGGLSLANLRSLGSNRTL
                     TLVDGRRHVGSNGGSLAVDVDTIPRLLIENVEIITGGASSVYGADAVSGVLNFVLRKD
                     FEGLEIDANYGMINQGGQANRRISILAGANLLDDRLNVWTFGEYDKNDQVKMLDIDWY
                     DEAWGTFGNDADPTAAANGPNNDGIFDNAGPVSDRRTLQILRWGQVTLANNQPASPLN
                     DPDVPFVNCPDAYTFNNLLAANCYNL"
     gene            complement(1194..>2441)
                     /gene="ftsA"
                     /locus_tag="VF686_00325"
     CDS             complement(1194..>2441)
                     /gene="ftsA"
                     /locus_tag="VF686_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX8660279.1"
                     /translation="LGQSKVACFIMKPEGVRHADRTIRVAGAGHVQSRGVRGGGIVNM
                     DEAAQAIGHAVERAERAAGSPVSGVVVTTAIGQMASHRVQARVSLGATPVGDADLARA
                     IAMALAQIRLPNRRPIHVLPIAWSVDGARGVHDPRSMKGHSLGLDLLVVSMAEGAFNG
                     LSHCLELAHLDLQGVASAPVVSSLAALEDDEMDLGCVCIDMGGGSTSAAVWGGRSLLH
                     IESLNVGGDHVTADIARGLSTSKAGAERLKTLHGSAMASANEDREMLEAPPRGEDPSA
                     GPIFVQRAMLKTVIAPRVEETLELLRDRLRNAGVGLEPGAGLVLTGGASQLNGVRELA
                     VRVFDRPVRLGKPQRAPHLADAASGPAFCSAAGVLLRAAYGPREAVSARKLMSRQLSA
                     ADAPRIHQGGIVARAAGWLRENL"
BASE COUNT          401 a          773 c          883 g          384 t
ORIGIN      
        1 aggttgtagc agttggcggc cagcaggttg ttgaaggtgt aggcatccgg gcagttgacg
       61 aacggcacgt ccggatcgtt cagggggctg gccggctggt tgttggccag ggtcacctga
      121 ccccaacgaa ggatctgcag cgtgcgacgg tcagacacgg gaccggcgtt gtcaaagatg
      181 ccgtcgttgt tcgggccatt ggcggccgcg gtcgggtcgg cgtcgttgcc gaaggtgccc
      241 caggcttcgt cataccagtc gatgtcgagc atcttgacct ggtcgttctt gtcgtattcg
      301 ccgaacgtcc agacgttcag gcggtcatca agaaggttgg cgcccgccag gatcgaaata
      361 cggcggttgg cttggccacc ttggttgatc atgccgtagt tggcgtcgat ctcgaggcct
      421 tcaaagtcct tgcgcaggac gaagttcagc acgccggaga cggcgtcggc accgtaaacc
      481 gacgaagcgc cgccggtgat gatctcgacg ttttcgatca ggaggcgcgg gatggtgtca
      541 acgtcaaccg ccagcgaacc accgttcgaa ccgacgtgac ggcgaccatc gaccagggtc
      601 agggtgcggt tggaacccag cgagcgcagg ttggccagcg acagaccgcc gtcacccagg
      661 ttcgagccgg tcgtgtccga aggcacaacc gagttcgaca gggccgggat ggtcgcaagg
      721 tagtcgatga cagtggactg accggtttcc agcagctgtt cgcggttgac cgtgatcagc
      781 ggggtcggag cgttcaccgg gtcgcggcgg atacgcgaac cggtgacgac gacttcgtcg
      841 atttcagtgg cttcgggatc ggcctggccc tggacgttca cggccggcag ttggctctgg
      901 gcgaacgccg gagcggcgac ggccatgacg ccggcgagga tcgtcgagct aaagaggtac
      961 ttgcgcttga gaagcatgat gttgggcccc agccaaagaa tggatgcaat cggcggcgcc
     1021 cgcctggtga cggtcgctgc ttcggaccgc cacgctaaca gtaaagaggc gagcggcaag
     1081 ggattgtgtc ctcttaacgg ccactccgac ctatcgttgt tacaatacgg acacagtgtc
     1141 gccacgcgaa tggcagccgc cgcgggcccc tctcggcccg gaggctggcg cgttcagagg
     1201 ttttcgcgaa gccaccccgc ggcgcgcgcc acaatcccgc cttggtggat gcgcggagca
     1261 tctgcagccg acagttgtct ggacatcagc ttgcgggccg agacagcttc ccgggggccg
     1321 taggcggcgc gtagcaggac gcccgcggcc gagcagaagg ccggtccgga ggccgcgtcg
     1381 gccaggtggg gcgcgcgctg aggcttgccc agacggacgg ggcggtcgaa cacgcgcacg
     1441 gccagttcgc gcacgccgtt cagttggctg gctccgccgg tcaggacgag acccgcgccg
     1501 ggctcgaggc ccacgccggc gttgcgcagg cggtcgcgca gcagctccag ggtttcctcg
     1561 acgcgcggag cgatgacggt cttcagcatg gcgcgctgga cgaagatcgg gcccgccgaa
     1621 gggtcctcgc cgcgcggcgg ggcctccagc atctcgcggt cctcgttggc gctggccatg
     1681 gccgagccgt gcagggtctt cagccgctcg gcgcccgcct tggaggtcga caggccgcgg
     1741 gcgatgtcgg cggtgacgtg gtcgccgccg acgttcagcg attcgatgtg cagcagggag
     1801 cgtccgcccc agaccgcagc cgaagtcgag ccgccgccca tgtcgataca gacgcagccg
     1861 aggtccatct cgtcgtcttc cagcgccgcc agcgacgaca ccaccggcgc cgaggccacg
     1921 ccctgaaggt cgaggtgcgc cagttcgagg cagtggctca ggccgttgaa cgcgccctcg
     1981 gccatggaga cgaccagcag gtcgaggccc agggaatggc ccttcatcga gcgaggatca
     2041 tgcacgccgc gggcgccatc caccgaccag gcgatgggca ggacgtggat cggacggcgg
     2101 ttgggcagac ggatctgggc cagggccatg gcgatggcgc gcgccagatc ggcgtcgccg
     2161 acgggcgtgg cgcccagaga aacccgagcc tggacgcggt ggctggccat ctggccgatg
     2221 gccgtggtga cgacgacgcc cgacaccggc gacccggcgg cgcgttcggc gcgctcgacg
     2281 gcgtggccga tggcttgggc cgcctcgtcc atattgacga tgcccccgcc ccgtacgccg
     2341 cgcgactgga catgccccgc acccgccacg cggatggtgc ggtcggcgtg gcgcacgccc
     2401 tcgggcttca tgatgaagca ggctaccttg gactggccga g
//
LOCUS       DASVVN010000017         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_26818, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000017 DASVVN010000000
VERSION     DASVVN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26818"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF686_00330"
     CDS             <1..499
                     /locus_tag="VF686_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX8660280.1"
                     /translation="MNRLVAILAVLAIAAPAWSQTAPAPAASPAAPAPPYGAPIGLEA
                     AQALIDRAVAVAGERRFRMAIAIVEPSGELVAFARMDDTQYGSIHVAQRKAATAARYR
                     GATSAVEERTLAGRTVTLANEDMLPIAGGVPIVVDGRIVGAIGVSGAAAAQDDEVARA
                     ALAAD"
     gene            544..1299
                     /locus_tag="VF686_00335"
     CDS             544..1299
                     /locus_tag="VF686_00335"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8660281.1"
                     /translation="MAFSRTYDGSEPVRINKWLGQSGVCSRREADALIADGLVSVDGE
                     VVTDAGRKLEPGQTLTLNERATAALAEGVTIVMHKPMGFVSGQPETDKIPAVRLITEA
                     NRVGEGAVPAEGASLPPIGRLDEDSRGLLLLSSDGVVAKAVIGPQSDLDKEYLVRVAG
                     DITEKKLKILRHGLMLDGRQLKPAYVSRMESFRLKFILREGRNRQIRRMCEMVDLEVV
                     DLIRVRIGPLKLDNLPEGRWRMLTAEERAALVG"
     gene            complement(1301..1837)
                     /locus_tag="VF686_00340"
     CDS             complement(1301..1837)
                     /locus_tag="VF686_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8660282.1"
                     /translation="MARIVFGMNVSLDGYVDHDRFEPGPELFRHWIEQVGGLTGSVYG
                     RRVYELMRYWDEDQPDWSPDLHAFAAAWRRQPKWVASRSLTSVGPNATLVKEDIEGVV
                     RGLKKDLEGEIDVSGPELAHSLGKAGLIDEYRLYFRPVVLGSGKPFFASAMPPLRLVA
                     HDLIGEDAMRLTYVPADG"
     gene            complement(1840..2520)
                     /locus_tag="VF686_00345"
     CDS             complement(1840..2520)
                     /locus_tag="VF686_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8660283.1"
                     /translation="MIQVTAFKWVPPFAQGSVRDIRVRWALEEAGLGYDDHLIGFEDQ
                     STPAYRAQQPFGQVPAFSDGKVEMFESGAIVLYIAQNSDQLMPADEAGRAMVMTWLFA
                     AMNSVEPFVGQLAGIDIFHADKAWAKERRPEVETMVRKRLADLQTALDARKWFANDRF
                     SAADILMAHVLRDLRHTDMLADYPALADYVARAEARPAFKRALADQLAPFRAAEAKNP
                     ELAAAQGN"
     gene            2572..>2897
                     /locus_tag="VF686_00350"
     CDS             2572..>2897
                     /locus_tag="VF686_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX8660284.1"
                     /translation="MSNAAANVPGPFADTLHIRDHCLCLHAQRAARTLARRFDEAFRD
                     LGVTSGQFSLLNGLNRPEPPTIGAVATLLAMDRSTVTANLKPLERMGAVVLGVDPKDR
                     RNRRPAL"
BASE COUNT          449 a          995 c         1013 g          440 t
ORIGIN      
        1 catgaaccgt ctcgtcgcca ttctggccgt tctggccatc gccgctccgg cctggagcca
       61 gaccgcccct gcccccgcag cgtcccccgc cgccccggcc ccgccctatg gcgcgccgat
      121 cgggctggag gcggcgcagg cgctgataga ccgggccgtc gccgtggcgg gagagcggcg
      181 gttccgcatg gccatcgcca tcgtcgagcc gtcgggcgaa ctggtcgcct tcgcccgcat
      241 ggacgacacc cagtacggct cgatccatgt cgcccagcgc aaggcggcga cggcggcgcg
      301 gtaccgcggc gccacgtcgg ccgtggagga gcggacgctg gcggggcgga cggtgacctt
      361 agccaatgaa gacatgctgc cgatcgcggg cggggtcccc atcgtggtcg acgggcggat
      421 cgtcggggcc atcggcgtat ccggcgccgc cgcggcccag gacgacgaag tggcgcgggc
      481 ggccctcgcc gcggactgac gccacgcttg ccggaggggg cgcccgaccg gtacggaccc
      541 cgcatggcct tctcccgcac ctatgacggc tccgagccgg tccggatcaa caaatggctg
      601 ggccagtcgg gcgtctgttc gcggcgcgag gccgacgccc tgatcgccga cggcctggtc
      661 tcggtcgatg gcgaggtggt caccgacgcg ggacgcaagc ttgagccggg ccagaccctg
      721 accctgaacg agcgggccac ggccgccctg gcggaagggg tgaccatcgt catgcacaag
      781 ccgatgggct tcgtctcggg ccagcccgag acggacaaaa tcccggcggt gcggctgatc
      841 accgaggcca accgggtcgg cgagggcgcg gttccggccg agggcgcctc cctgcccccg
      901 atcgggcggc tggacgagga ttcccggggc ctgctgctgc tgtcgtcgga cggggtggtg
      961 gccaaggcgg tgatcgggcc gcagtccgat ctggacaagg aatatctggt ccgggtcgcc
     1021 ggcgacatca ccgagaagaa gctgaagatc ctgcgtcacg gcctgatgct ggacggccgt
     1081 cagctgaagc ccgcctacgt cagccgcatg gaatccttcc ggctgaaatt catcctgcgc
     1141 gaggggcgca accggcagat ccgccggatg tgcgagatgg tcgatctgga ggttgtcgac
     1201 ctgatccgcg tccggatcgg gccgctgaag ctggacaatc tgcccgaggg ccgatggcgg
     1261 atgctgacgg ccgaggagcg ggcggcgctg gtcggctgag tcaaccgtca gcaggaacgt
     1321 aggtcagccg catcgcgtcc tcgccgatca ggtcgtgggc gacgaggcgc agcggcggca
     1381 tggcgctggc gaagaagggt ttgccgcttc ccaggacgac gggacggaaa tagagccggt
     1441 actcgtcgat gagacccgcc ttaccaaggc tgtgcgccag ttccgggccg ctgacgtcga
     1501 tctccccctc gaggtccttc ttcaacccac gaaccacgcc ctcgatgtcc tccttgacga
     1561 gcgtcgcgtt ggggccgacg gaggtcagcg aacgcgacgc cacccatttg ggctgtctgc
     1621 gccatgccgc cgcaaaggcg tgcaggtccg ggctccagtc gggctggtcc tcgtcccaat
     1681 aacgcatgag ctcgtagacc cggcggccgt agacgctgcc cgtcagcccg cccacctgct
     1741 cgatccagtg ccggaacagc tcggggccgg gctcgaaccg gtcgtggtcg acgtagccgt
     1801 ccagcgacac gttcataccg aagacaatcc gcgccatgct cagtttccct gcgccgcggc
     1861 cagctccggg ttcttcgcct ccgccgccct gaacggcgcc agctggtcgg ccagggcgcg
     1921 tttgaaggcc gggcgggcct cggcccgggc gacatagtcg gccagcgcgg gatagtcggc
     1981 gagcatgtcc gtgtggcgca ggtcgcgcag gacgtgggcc atcaggatgt cggcggcgct
     2041 gaagcggtcg ttggcgaacc atttgcgggc gtccagggcg gtctggagat cggccagacg
     2101 cttgcgcacc atggtctcga cctccggccg ccgttccttc gcccacgcct tgtcagcgtg
     2161 gaagatatca attcccgcca gctgcccgac gaagggctcg acgctgttca tcgccgcgaa
     2221 cagccaggtc atgaccatgg cccgccccgc ttcgtcggcc ggcatcagct ggtccgagtt
     2281 ctgcgcgatg tagagcacga tggcgccgct ctcgaacatc tcgaccttgc cgtcgctgaa
     2341 ggccggcacc tgaccaaacg gttgctgcgc gcgataggcg ggcgtcgact ggtcctcgaa
     2401 accgatcaga tggtcatcat agccgagacc cgcctcctcc agcgcccagc ggacgcggat
     2461 gtcgcggacc gagccctggg cgaagggcgg cacccattta aacgcggtga cctggatcat
     2521 gacggctcct caggatttga cagttacgtt gatatcaaca tatttgagag catgtcaaac
     2581 gccgcagcca acgtcccggg gcccttcgcc gacaccctgc acatccggga tcactgcctg
     2641 tgcctgcacg cgcagcgggc ggcgcggacg ctggcgcggc ggttcgacga ggcgttccgc
     2701 gacctgggcg tcaccagcgg ccagttctcg ctgctgaacg gcctgaaccg gcccgagccg
     2761 ccgaccatcg gcgcggtggc cacgctgctg gccatggacc ggtcgaccgt gacggccaat
     2821 ctgaagccgc tggagcggat gggggcggtc gtgctgggcg tcgatccgaa ggaccggcgc
     2881 aaccgccgcc ctgccct
//
LOCUS       DASVVN010000018         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_263662,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000018 DASVVN010000000
VERSION     DASVVN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263662"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..250
                     /locus_tag="VF686_00355"
     CDS             38..250
                     /locus_tag="VF686_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8660285.1"
                     /translation="MKTIVKLAPVAAVLTLLGACGSTMEQRAATGAIGGAVVGQAIGG
                     NTGSTVAGAAAGAVAGAVTTPRRRPN"
     gene            complement(323..>1621)
                     /locus_tag="VF686_00360"
     CDS             complement(323..>1621)
                     /locus_tag="VF686_00360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8660286.1"
                     /translation="DKIYNPATLAELAASAPGFDWSAWAHGAGISHVTTLIAANNTAF
                     PGMARVFAETPVATLQSWQAFHVVDQASPWLSSAFVDNNFNFYGKVLGGQPENRPRWK
                     RGVAQVDSSLGEVLGREFVARHFPAESKAQMETLVANLRRAMTARIEGLDWMSPETKQ
                     QALYKMSRFGVKIGYPDEWREYDGLRLEPGDLYGNVERSAAFEWAFNVNKLGKPVNPL
                     EWGMTPQTVNAYYNPVRNEIVFPAAILQAPFFDPNADPAVNYGGIGAVIGHEITHGFD
                     DQGRKTDGDGVLRDWWTPEDAAKFEARAAVLGAQYAALSPLEGANVNADLTMGENIAD
                     LGGLLLALDAYHMSLNGAEAPVINGLTGDQRVFLGWAQVWRNNIRDEALRQQVQVDPH
                     SPAQYRAAVSPRNIDAWYAAFGVQPGDEQYLAPEARARIW"
BASE COUNT          237 a          529 c          590 g          265 t
ORIGIN      
        1 cgttttcatg gcgcctttcc tcccaaggag aaccaagatg aagacgatcg taaaactggc
       61 tcccgtggcc gcggtgctga ccctgctggg cgcctgcggc tcgaccatgg aacagcgcgc
      121 cgccaccggc gccatcggcg gcgccgtcgt cggccaggcc atcggcggca acacgggctc
      181 gacggtggcc ggcgcagcgg ccggcgccgt ggccggggcg gtgaccacgc cgcgccgccg
      241 tccgaactaa accaaccttc cggccaaacg aaaacggccc cgacgagaga tcgccggggc
      301 cgtcgtcatt gtgcggtccg ccttaccaga tgcgggcgcg ggcctcgggg gcgaggtatt
      361 gctcgtcgcc cggctggaca ccgaacgccg cataccaggc gtcgatgttc cgcggcgaca
      421 ccgctgcccg gtactgggcc ggggagtgcg gatcgacctg cacctgctgg cgcagggcct
      481 cgtcccggat gttgttgcgc cacacctggg cccagccgag gaagacgcgc tggtcgccgg
      541 tcaggccgtt gatcaccggc gcttccgccc cgttcagcga catgtggtag gcgtcgaggg
      601 ccagcagcag gccgccgagg tcggcgatat tctcgcccat ggtcaggtcg gcgttgacgt
      661 tggcgccttc cagcgggctg agagcggcgt attgcgcacc cagaacggcg gcgcgggcct
      721 cgaacttggc ggcgtcctcg ggcgtccacc agtcgcgcag cacgccgtcg ccatcggtct
      781 tgcggccctg gtcgtcgaag ccgtgggtga tctcgtggcc gatgacggcg ccgatgccgc
      841 cgtagttgac cgccggatcg gcgttcggat cgaagaaggg cgcctgcagg atggcggcgg
      901 ggaagacgat ctcgttgcgg acggggttgt agtaggcgtt caccgtctgc ggggtcatgc
      961 cccactccag cgggttcacc ggcttgccca gcttgttgac gttgaaggcc cattcgaagg
     1021 ccgccgaacg ctcgacgttg ccgtacaggt cgcccggctc gagacgcagg ccgtcgtatt
     1081 cgcgccattc gtcgggatag ccgatcttca ccccgaaacg gctcatcttg tagagggcct
     1141 gctgcttcgt ttcggggctc atccagtcga ggccctcgat gcgggcggtc atggcgcggc
     1201 gcaggttggc gaccagggtc tccatctggg ccttggactc ggcggggaag tgacgcgcca
     1261 cgaactcgcg gcccagcacc tcgcccagcg agctgtcgac ctgggcgacg ccgcgcttcc
     1321 agcggggccg gttctcgggc tgaccgccga ggaccttgcc gtagaagttg aagttgttgt
     1381 cgacgaaggc gctcgacagc cagggcgagg cctggtcgac gacgtggaag gcctgccacg
     1441 attgcagggt tgcgaccggc gtttcggcga acacgcgagc catgcccggg aaggcggtgt
     1501 tgttggcggc gatcagggtc gtgacgtgac tgatgccggc gccgtgggcc caggccgacc
     1561 agtcgaagcc gggcgccgag gcggccagtt cggcgagggt ggccgggttg tagatcttgt
     1621 c
//
LOCUS       DASVVN010000019         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_395399,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000019 DASVVN010000000
VERSION     DASVVN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395399"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="VF686_00365"
     CDS             complement(<1..148)
                     /locus_tag="VF686_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX8660287.1"
                     /translation="MTTRDLEGWTIAFDLDGTLVDTAPDLIGALNAVLEEEQLPPAPL
                     ESARN"
     gene            215..1567
                     /gene="glmU"
                     /locus_tag="VF686_00370"
     CDS             215..1567
                     /gene="glmU"
                     /locus_tag="VF686_00370"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX8660288.1"
                     /translation="MTSTHPRAAIILAAGQGTRMKSALPKVLHPVGGRAMLDHAIDAA
                     EALGCERIVVVVGAHSPEVRDHVVRRLGEDAIAVQDPPLGTGHAVRAAEAVLGDFVGQ
                     VVITYGDVPLLRAADIEPVFAAHEGVTVIGFEARDPGAYGRLLMEGDTLAAITEAKEA
                     SPEVLALTACNSGVMAAPVGLLFELLAEVRNDNAKGEYYLTDVVELARKRGAPTRAVF
                     AGEDAVMGVNAQGELAVAEALFQKVQRETFLAAGVTMPAPETVHFSFDTQVGGGTTIE
                     PYVVFGPGAKIASGARIRSFSHIEGATVASGAEVGPYARLRPGADLGEGAKVGNFVEV
                     KNVRMGKGAKANHLAYLGDGEVGAKANIGAGTIFCNYDGFFKHRTTVGEGAFVGSNSS
                     LVAPITIGAGAMVGSGSVITRDVAPGDLALARGEQQAKTGWAARFMEKMRAKKALKAK
                     "
     gene            1564..2250
                     /gene="rpiA"
                     /locus_tag="VF686_00375"
     CDS             1564..2250
                     /gene="rpiA"
                     /locus_tag="VF686_00375"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX8660289.1"
                     /translation="MSVDDQKRRAGAAAAERVEPGIVVGLGTGSTAAWFVKALAARNL
                     TGLRCVPTSEATAELARELGLTLSTLEDTPRIDLTVDGADEIGPGLALIKGGGAALLR
                     EKLVWEASARCIVIADAAKVVQTLGAFALPIEVVAFGHKTTANRIADVLSDHDIAMPA
                     RVRMAERGLVRTDGGNLIYDAKCGVIHDPVRLADDLKLITGVVEHGLFLDLADEAIIG
                     RDDGVEVLTP"
BASE COUNT          363 a          801 c          833 g          354 t
ORIGIN      
        1 ggttgcgcgc actctccagc ggggcgggcg gcagctgctc ctcctccagc acggcgttca
       61 gggcgccgat caggtcgggg gcggtgtcga cgagcgtgcc gtcgaggtcg aaggcgatgg
      121 tccagccttc taggtcgcgc gtcgtcatcc acatctcccg ccggtcgccg ggatgggcta
      181 gaccgctggc gacgactagc cgccggaccg attcatgacc agcacccacc ccagagccgc
      241 catcatcctc gccgcgggcc aaggcacgcg gatgaagtca gccctgccca aggtgctgca
      301 cccggtcggc ggccgcgcca tgctggatca cgccatcgac gccgccgagg cgctgggctg
      361 cgagcggatc gtggtggtcg tcggcgccca ttcgcccgag gtgcgcgacc atgtcgtcag
      421 acggctgggc gaggacgcca tcgccgtcca ggatccgccg ttgggcaccg gccatgcggt
      481 ccgcgccgcc gaggccgtgc tgggcgattt cgtcggacag gtggtgatca cctatggcga
      541 cgtgccgctg ctgcgcgccg cggatatcga accggtcttc gcagcgcatg agggcgtcac
      601 cgtcatcggc ttcgaggccc gcgaccccgg agcctatggg cggctgctca tggagggcga
      661 cacgttggcc gccatcacgg aagcgaagga ggcttcgccc gaggtcctgg ccctgaccgc
      721 ctgcaactcg ggcgtcatgg cggcgcctgt cggcctgctg ttcgaactgc tggccgaggt
      781 ccgaaacgac aacgccaagg gcgaatacta tctgaccgac gtggtcgagc tggcccgcaa
      841 gcgcggcgct ccgacccgcg ccgtgttcgc cggtgaggac gccgtcatgg gggtgaacgc
      901 ccaaggcgaa ctggccgtgg ccgaggctct gttccagaag gtccagcgcg agaccttcct
      961 cgccgccggc gtcaccatgc cggccccgga gacggtgcat ttctcgttcg acacacaggt
     1021 cgggggcggg acaactatcg agccctacgt cgtgttcggt cccggcgcga agatcgcctc
     1081 aggcgcccgc attcgcagct tcagccatat cgagggcgct accgtagctt ccggcgccga
     1141 ggtcggtccc tacgcccgcc tgcgtcccgg cgcggacctt ggggagggcg ccaaggtcgg
     1201 caacttcgtc gaggtgaaga acgtccgcat gggcaagggc gccaaggcca accatctggc
     1261 ctatctcggc gacggcgagg tgggcgccaa ggccaacatc ggcgcgggca cgatcttctg
     1321 taactacgac ggcttcttca aacaccgcac gacggtgggc gagggggctt tcgtcggctc
     1381 gaacagttcg ctggtcgccc cgatcaccat cggtgcgggc gccatggtcg gctcgggctc
     1441 ggtgatcacc cgcgacgtgg cccccggtga tctggcgctg gcccgcggcg agcagcaggc
     1501 caagaccggc tgggctgcgc gcttcatgga aaagatgcgg gcgaagaagg cgttgaaagc
     1561 gaaatgagcg tggacgatca gaaacgccgg gcgggagcag ccgccgctga acgggtcgag
     1621 cccggcattg tcgtcggcct cggcaccggc tccaccgccg cctggttcgt caaggcgctg
     1681 gccgcgcgca acctgaccgg cctgcgctgc gtgccgacgt cggaggcgac ggccgaactg
     1741 gcgcgcgaac tggggctgac gctgtcgacg ctggaggata cgccgcgcat cgacctgacc
     1801 gtcgacgggg cggacgagat cggacccggg ctggccctga tcaagggtgg cggcgcggcc
     1861 ctgctgcgcg aaaagctggt gtgggaggcc tcggcccgct gcatcgtcat cgccgacgcc
     1921 gccaaggtgg tgcagacgct gggcgccttc gccctgccga tcgaggtcgt cgccttcggc
     1981 cacaagacga ccgccaaccg catcgccgac gtgctgtcgg accatgacat agccatgccg
     2041 gcgcgggtgc gcatggcaga gcgcggtctg gttcgcaccg acggcggcaa cctcatctac
     2101 gacgcgaaat gcggcgtcat ccacgatccg gtgcgtctgg ccgacgatct gaagctgatc
     2161 accggcgtgg tcgaacacgg cctgttcctc gatcttgcgg acgaggcgat catcggccgc
     2221 gacgacggcg tcgaggtgct gacgccctga ccgcctgccg ctgacggaag cgtcagtcgg
     2281 cttcagcctc ctgcaggtcc tcatgcgccc ggtggatcga ggcgacgact tccgtacgcg
     2341 ccgcgaggcc g
//
LOCUS       DASVVN010000020         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_211245,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000020 DASVVN010000000
VERSION     DASVVN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211245"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /gene="ispH"
                     /locus_tag="VF686_00380"
                     /gene_synonym="lytB"
     CDS             complement(<1..211)
                     /gene="ispH"
                     /locus_tag="VF686_00380"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523440.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8660290.1"
                     /translation="MTVHAPIHPADKPALTVRLATPRGFCAGVDRAIQIVERAIEKYG
                     APVYVRHEIVHNRHVVERLKALGAVF"
     gene            374..1081
                     /locus_tag="VF686_00385"
     CDS             374..1081
                     /locus_tag="VF686_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="HEX8660291.1"
                     /translation="MRRVLKVSLASALAAALAVVTVPTAANAQYGSPGQQNRPGQTSR
                     PGQDQQQPGEQSDRPRPARIAPLRERAAAGPCPYVKILYDAARYVELTGDRVAAANVG
                     YTGEIEGLVSDCGYMNDEPITVETRILFNLGRGPQAQGDGRTYRYWIAVTERNRAILA
                     KEYFDLPVSFEGEETATVTQDQTIVIPRATATTSGDHFEILVGFDVTPQMAEFNRSGS
                     RFRVNAGTPATTPPPAQ"
     gene            1082..2884
                     /gene="argS"
                     /locus_tag="VF686_00390"
     CDS             1082..2884
                     /gene="argS"
                     /locus_tag="VF686_00390"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8660292.1"
                     /translation="MTDLRTVLGEAVGAAFAAEGVDPALARVTTSDRPDLADFQSNGA
                     LAAAKALKANPRELATRVAERLTGDPRLSSVEVAGPGFINLKLTDSVLSARAAEVAAD
                     TDHSGASMVTSPRKVVIDFGGPNVAKPMHVGHLRSSIIGESLKRLFRFRGDTVWGDAH
                     FGDWGFQMGLLIVAVGDEGKADDFLAEGDGPFPAASPVSLDDLERLYPAAAAMAKEDA
                     NFRDRARKATAELQGGRPGYRALWSHFVAVSRTALEREFGALGVTFDLWNGESDADPV
                     MAEMIAHLEKTGLLVEDDGARVVHVARPGETRKKKLADGSVIEAPSPPPLLVISSEGS
                     AMYGTTDLATILDRRKAIDPDLILYVVDERQAEHFEQVFRAAYLAGYAAEGSLDHLGF
                     GTMNGPDGKPFKTRAGGVLKLHDLITMAVEKARDRLHEAHLGEDLSEAEFEAIAHKVA
                     VAALKFADLSNSRTTSYVFDLDRFMSFEGKTGPYLLYQAVRIKSLLRKGADQGAAPGP
                     IVIAEPAERDLALTLDAFSQAVAEAYDKRMPHVVAEHAYRLAQSFSKFYAACPVLIAP
                     DKATRGSRLALSKAALDQLECALRLLGIDTPERM"
     gene            2917..>3212
                     /locus_tag="VF686_00395"
     CDS             2917..>3212
                     /locus_tag="VF686_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX8660293.1"
                     /translation="MSLDAYIAGLPKAELHLHIEGSLEPELMFELAQRNGVAIPFDSV
                     EAVRAAYDFSNLQDFLDIYYAGANVLLTRRDFEDLAFAYFQRAAADNVRHAEIF"
BASE COUNT          529 a         1126 c         1054 g          503 t
ORIGIN      
        1 cgaagacggc gccgagggcc ttgagccgct caaccacgtg gcggttgtgg acgatctcgt
       61 gccgaacgta gacgggcgcg ccgtatttct cgatggcccg ttcgacgatc tggatggccc
      121 ggtcgacgcc cgcgcagaag ccgcgcggcg tggccagacg cacggtcagg gcgggcttgt
      181 ccgccgggtg gataggcgcg tgaacggtca tggctggaac ctagtcgttc gaaggccccg
      241 ccgccacctg catccggctc cgggtttccg cgcgcgcggg tttgccgcgc ctcggtttag
      301 ccgctatcag cggcgagcgt gccgcccggg cgtcgggctg tcgccgcacc tcctcgtttc
      361 cggaagacac tgcatgcgtc gcgtcctcaa ggtttccctg gcctcggccc tcgccgccgc
      421 gctcgcggtc gtgactgtgc cgaccgcggc caacgcccaa tacggctcgc ccggccagca
      481 gaaccggccg ggccagacca gccgtccggg tcaggatcag caacagccgg gcgaacagag
      541 cgacaggccg cgtcctgcgc gtatcgcacc cctgcgcgaa cgggccgccg ctggcccctg
      601 cccctatgtg aagatcctgt acgacgcggc ccgctacgtc gaactgaccg gcgaccgggt
      661 ggccgccgcc aacgtcggct ataccggcga gatcgaaggt ctggtgtcgg actgcggcta
      721 catgaacgac gagccgatca cggtagagac gcggatcctg ttcaacctgg ggcgcggacc
      781 gcaggcccag ggcgacggcc gcacctatcg ctactggatc gccgtgaccg agcggaaccg
      841 cgccatcctg gccaaggaat atttcgacct gcccgtcagc ttcgagggcg aggagaccgc
      901 cacggtgacc caggaccaga ccatcgtcat cccgcgcgcc accgccacga ccagcggcga
      961 ccatttcgaa atcctggtcg gcttcgacgt cacgccgcag atggccgagt tcaaccgctc
     1021 gggtagccgg ttccgcgtca atgcgggcac gccggcgacc acgcctcccc cggcccagta
     1081 aatgaccgac ctcagaactg tcctgggcga agcggtcggg gccgccttcg ccgccgaagg
     1141 cgtggacccc gcgctggccc gggtgaccac atccgatcga ccggatctgg ccgacttcca
     1201 gtcgaacgga gccctcgccg ccgccaaggc gctgaaggcc aatccccgcg agctggcgac
     1261 ccgcgtcgcc gagcggctaa ccggcgatcc gcgcctgtcc tcggtcgagg tcgcgggacc
     1321 cggcttcatc aatctgaagc tgactgatag cgtcctgtcg gcgcgcgcgg ccgaggtcgc
     1381 ggccgacacg gaccattctg gcgcctcaat ggtcacctca ccgcgcaagg tcgttatcga
     1441 cttcggcggg cctaacgtcg ccaagccgat gcacgtcgga catctgcgct cgtccatcat
     1501 cggcgaaagc ctgaagcggc tgttccgttt ccgcggcgac acggtctggg gcgacgccca
     1561 tttcggcgac tggggtttcc agatgggcct gctgatcgtc gccgtgggcg acgagggcaa
     1621 ggctgacgat tttctggcgg agggcgacgg tcccttcccc gccgcaagcc cggtctcact
     1681 tgacgatctc gagcgcctct atccggccgc cgccgccatg gccaaggagg acgcgaactt
     1741 ccgggaccgc gcccgcaagg cgacggcgga gctacaaggc ggacgtcccg gttatcgcgc
     1801 cctctggtcc catttcgtcg cggtcagccg tacggcgctg gaacgcgagt tcggcgccct
     1861 gggcgtgacc ttcgacctgt ggaacggcga gagcgacgcc gacccggtga tggccgagat
     1921 gatcgcacac ctcgagaaga ccggcctgct ggtcgaggac gacggcgccc gcgtggttca
     1981 cgtcgcccgc cccggcgaga cccgcaagaa gaagctggcc gacggctcgg tgatcgaggc
     2041 gccaagcccg ccgccgctgc tggtgatcag ttcggaaggc tcggccatgt acggcacgac
     2101 cgatctggcc accatcctgg accgccgcaa ggccatcgac cccgacctga tcctgtacgt
     2161 cgtcgacgaa cggcaggcgg agcatttcga acaggtgttc cgcgcggcct atctggccgg
     2221 ctatgcggcc gagggttcgc tggaccacct cggcttcggc accatgaacg ggccggacgg
     2281 caagcccttc aagacccgcg cgggcggcgt cctgaaactg cacgacctga tcaccatggc
     2341 ggtggagaag gcgcgcgacc gactgcacga ggcgcatctc ggcgaggacc tgtccgaggc
     2401 cgagttcgaa gccatcgccc acaaggtggc cgtggccgcc ctcaaattcg ccgatctgtc
     2461 gaacagccgg acaaccagct acgtcttcga tctcgaccgc ttcatgagct tcgagggcaa
     2521 gaccggaccc tatctgctgt accaggcggt gcggatcaaa tccctgctgc gcaagggcgc
     2581 ggaccagggg gcggcgcccg gccccatcgt catcgccgag cctgccgaac gggatctggc
     2641 cctgacgctg gacgccttct cgcaggcggt cgccgaagcc tatgacaagc ggatgccgca
     2701 cgtcgtggcc gagcacgcct accggctggc gcagtcattc tcgaaattct acgccgcctg
     2761 tccggtgctg atcgcgccgg acaaagcgac gcggggttcg cgtctggcgc tgtcgaaagc
     2821 cgcgctggac cagctggaat gcgcgctccg cctgttggga atcgacacgc ccgagcggat
     2881 gtaggctaga ctggcggcct gtttccggag ccgcccatgt cgctggacgc ctatatcgcc
     2941 ggcctgccca aggccgaact gcatctgcac atcgagggca gccttgagcc cgaactgatg
     3001 ttcgaactgg cgcagcggaa cggcgtggcc attccattcg acagcgtcga ggcggtgcgc
     3061 gccgcctatg acttttcgaa cctgcaggac ttcctcgaca tctattacgc tggcgcgaac
     3121 gtgctgctga cgcggcgaga tttcgaggat ctcgccttcg cctatttcca gcgggcagcc
     3181 gcggacaacg tccgccatgc cgaaatcttc tt
//
LOCUS       DASVVN010000021         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_26954, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000021 DASVVN010000000
VERSION     DASVVN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26954"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF686_00400"
     CDS             <1..309
                     /locus_tag="VF686_00400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX8660294.1"
                     /translation="PSILNASPEAATGGNIALLKTGDRMRIDLNTRRVDVLIDEAGMA
                     DRRKALEAAGGYQYPASQTPWQEFQRGFVGELETGAVLEPAVKYHRIVDTLGLPRDNH
                     "
     gene            328..1872
                     /locus_tag="VF686_00405"
     CDS             328..1872
                     /locus_tag="VF686_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8660295.1"
                     /translation="MERATVPAGGIRRLVIVGGGTAGWMAAAALAKLVSPTGVEVALI
                     ESAEIGTVGVGESTLPHIRDFNARLGIDEAEFMRATRATFKLGIEFKDWARIGDRYMH
                     PFGDFGAPGGSAPFHHYWLAARELADVGPLDDYAVPIVAAWAARFGPPDQDPRSILST
                     YDYAYQLDASLYAQFMRRFAEGLGVTRIEGRIVDVSQDGESGAIRSVALERGETVAGD
                     LFIDCSGFRGLLIEQTLKSGYTDWSHWLPCDRAVAMPCAGVGRTEPYTRSAAMGAGWR
                     FRIPLQHRIGNGYIYSSAFIDRDAATADLMSNLEGEALNTPNHLRFTAGQRTRNWVGN
                     CVSMGLASGFIEPLESTSIYLIQAAITKLIELWPAHPLDAVAAKEFNRQMDLQYERIR
                     DFIILHYKATERDDTPFWNHVRAMSVPDSLAEKIELFREKGVVQEYREGLFQHPSWVS
                     VYVGQRIMPRTRHMLADAENAERLVAGLQRLRAAVAQAAGRLALQDDYIAGYCAMRDE
                     AAAPAA"
     gene            complement(1830..>3755)
                     /locus_tag="VF686_00410"
     CDS             complement(1830..>3755)
                     /locus_tag="VF686_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX8660296.1"
                     /translation="QTAPQRAMSPRRTGLAIVAVLVAAASAATAQVSDPSPVRMNQAG
                     FETTGPKAATVPSTATMPLPWRVVDTAGATVAEGVTSVFGADAASGETVHQIDFPTLT
                     RPGDSYRLEVDARTSRPFGIAARPFARLKYDAMAYFYQNRAGVPISADHVARSDLARP
                     AGHARETATCFSGVDQRGTTWPGCDYTLDVTGGWYDAGDHGKYVVNGGISVWTLVNAA
                     ERARRLGQGAFADGRLDVPEAGNGVDDLLDEARYQIAFLLRMQVPEGATAWVPDGPLP
                     PGGAPLQLVEIDAGGMAHHKVHDRNWTSLPTAPADASAERLLYPPSTAATLNLAAVAA
                     QCARVWRDVDPAFSATCLSSARRAWRAARRHPAVFASSAFDGGGGYGDARVADEFYWA
                     AAELFAATGNAGYLAALKASPHWLGGPRSGASATGDPAWPATAALGTITLATVPTALD
                     PADRDTARRHLLEAARAYLAEGRTQGYGLPRGGTRFEWGSNGGLMNRAMMLGLAHDFT
                     GEAAFRDGVIQSLDFLLGRNPLDRSFVSGWGARPMVNPHHRFWAHSADPKYPSPPAGS
                     LSGGPNNTAMTDDVARTMVGQCAPLMCWVDDLRAYALNEVAINWNAPLVWVAAFIDPA
                     PMDDQAAGAAASSRIAQ"
BASE COUNT          580 a         1251 c         1380 g          544 t
ORIGIN      
        1 ccctcgatcc tgaacgcctc gcccgaggca gccaccggcg gcaatatcgc cctgctgaaa
       61 accggcgacc ggatgcggat cgacctcaac acacgccggg tcgacgtcct gatcgatgag
      121 gccgggatgg ccgaccgccg caaggctctg gaggccgccg gcggctacca gtatcccgcg
      181 tcccagacgc cgtggcagga gttccagcgc ggcttcgtcg gcgaactgga aaccggggcg
      241 gtgctggagc cggcggtcaa ataccatcgg atcgtcgaca ccctcggcct tccgcgcgac
      301 aaccactgaa agcgggcggg ggcgatcatg gaaagggcga ctgttcccgc gggcggcatc
      361 cgccgcctcg tcatcgtcgg aggcggcacc gccggctgga tggccgccgc ggcgctggcc
      421 aaactggtct cgccgaccgg cgtagaggtg gccctgatcg agtccgccga gatcgggacc
      481 gtcggcgtcg gggaatcgac cctgcctcac atccgggact tcaatgcccg cctcgggatc
      541 gacgaggccg agttcatgcg cgccacccgg gcgacattta agctgggcat cgagttcaag
      601 gattgggcgc ggatcggcga ccgctacatg catccgttcg gggatttcgg cgcgcccggc
      661 ggcagcgcgc ccttccacca ctactggctg gcggcgcgcg aactggccga tgtcgggccg
      721 ctggacgact acgcggtgcc gattgtggcg gcctgggccg cgcggttcgg gccgccggac
      781 caagacccgc gatcgatcct gtcgacgtac gactacgcct accagctgga cgccagcctc
      841 tatgcgcaat tcatgcggcg gttcgccgag gggctgggcg tcacgcggat cgagggcagg
      901 atcgtcgacg tcagccagga cggcgagtcg ggcgcgatca ggtccgtggc gctggagcgc
      961 ggcgagaccg tcgccggcga cctgttcatc gactgctcgg gtttccgcgg cctgctgatc
     1021 gaacagaccc tgaagtccgg ctacacggac tggtcccact ggctgccgtg cgaccgggcc
     1081 gtggccatgc cctgcgcagg cgtcggccgg accgagccct acacccggtc agcggccatg
     1141 ggcgcggggt ggcggttccg cattccgctg cagcaccgca tcggcaacgg ctacatctac
     1201 agcagcgcct tcatcgaccg ggacgccgcg accgcggacc tgatgtcgaa cctggaaggc
     1261 gaggcgttga atacgcccaa ccacctgcgg ttcacggcag ggcagcggac ccgaaactgg
     1321 gtcggcaatt gcgtttcaat gggactggcc tcaggcttca tcgagcccct ggagtcgacc
     1381 agcatctatc tgatccaggc cgcgatcacc aagctgatcg agctgtggcc ggcgcatccg
     1441 ctggacgccg tcgcggcgaa ggaattcaat cgccagatgg acctgcaata tgagcggatc
     1501 cgcgacttca tcatcctgca ctacaaggcc accgagcggg acgacacgcc gttctggaac
     1561 cacgtccgcg ctatgagcgt gcccgacagt ctggcggaga agatcgagct gttccgcgaa
     1621 aagggcgtgg tccaggaata ccgcgagggg ctgttccagc acccgtcatg ggtctcggtc
     1681 tatgtgggac agcggatcat gccccggacc cggcacatgc tggcggacgc cgaaaatgcc
     1741 gaacgtctgg tcgcggggtt gcagcgcctg agggcggctg tcgcgcaggc ggcggggcgc
     1801 ctggccctgc aggacgacta tatcgcgggc tattgcgcga tgcgggacga ggcggcggcg
     1861 cccgcggcct gatcgtccat cggtgcggga tcgatgaagg cggcgaccca gaccaggggc
     1921 gcgttccagt tgatggcgac ctcgttcagc gcataggcgc gcaggtcgtc gacccagcac
     1981 atcaggggtg cgcactgccc gaccatggtc cgcgcgacgt cgtcggtcat cgccgtgttg
     2041 ttgggtcccc ccgacaatga gccggcgggc ggagagggat acttcggatc ggctgagtgg
     2101 gcccagaacc ggtgatgcgg attgaccatc ggtcgcgcgc cccatccgct gacgaaagag
     2161 cggtccagcg ggttgcggcc cagcaggaag tcgagggact ggatcacgcc gtcccggaac
     2221 gccgcctcgc cggtgaagtc atgggcgagg ccgagcatca tggcccggtt catcagcccg
     2281 ccgttcgagc cccattcgaa ccgggtcccg ccccgcggca ggccatagcc ctgggtgcgg
     2341 ccctcggcga ggtacgctcg cgcggcctcg agcagatgcc ggcgggccgt gtcgcggtcg
     2401 gccgggtcca gcgcagtcgg gaccgtggcc agggtgatgg ttcccagcgc ggcggtggcg
     2461 ggccaggcgg ggtcgccagt ggccgaggcg cccgagcgtg ggccgcccaa ccagtggggc
     2521 gaggccttga gcgccgcgag gtatccggca ttgccggtag ccgcgaacag ctcggccgcg
     2581 gcccagtaga actcgtcagc gacccgggcg tcgccatagc ccccgccgcc gtcaaaggcg
     2641 ctgcttgcga agacggcggg atgacgccgc gccgccctcc aggcgcgtct cgccgagctc
     2701 aggcaggtag ctgagaaggc cgggtcgacg tcgcgccaga cccgggcgca ctgggcggcg
     2761 acggcggcga ggttcagggt cgcggcggtc gagggcgggt acagcagccg ctccgccgac
     2821 gcatcggccg gggcggtggg aagcgaggtc cagttgcggt catggacctt gtgatgggcc
     2881 atgccgccgg cgtcgatttc gaccagctgc aagggcgcgc ctccgggggg cagggggccg
     2941 tcggggaccc aggctgtcgc gccctccggc acctgcatcc gcagcaggaa ggcgatctgg
     3001 tagcgcgcct cgtcgagcag gtcgtcgacg ccgtttccgg cctcgggaac gtccagacgg
     3061 ccgtcggcga acgcgccctg accgagcctc cgcgccctct ccgccgcatt gaccagggtc
     3121 cagacggaaa tgcccccgtt gacgacgtat ttcccgtggt cgcccgcgtc gtaccagccg
     3181 cccgtgacgt cgagcgtata gtcgcagccg ggccaggtcg tgccgcgctg atcgacgccg
     3241 gagaagcagg ttgcggtctc gcgcgcatgg cccgccggcc gggccagatc ggagcgggcg
     3301 acgtggtccg ccgagatcgg gacgccggcc cggttctgat agaaataggc catggcgtcg
     3361 tacttcagcc gcgcgaaggg ccgcgccgcg atcccgaacg gacggctggt ccgggcgtcg
     3421 acctcgaggc ggtagctgtc gcccggacgg gtcagggtcg ggaagtcgat ctggtggacc
     3481 gtctctccgg aggcggcgtc ggctccgaag accgaggtga ctccctctgc gacggtcgcg
     3541 ccggcggtgt cgacgacgcg ccaggggagg ggcatggtcg cggtcgaggg gacggttgcg
     3601 gccttggggc ctgtcgtctc gaacccggcc tggttcatgc gcacgggcga tggatcagag
     3661 acctgggccg tcgcggcgga ggcggcggcg acgaggaccg cgacgatggc cagacccgta
     3721 cggcgcgggc tcatggctcg ttgcggggca gtttg
//
LOCUS       DASVVN010000022         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_211276,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000022 DASVVN010000000
VERSION     DASVVN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211276"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF686_00415"
     CDS             complement(<1..228)
                     /locus_tag="VF686_00415"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168979.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8660297.1"
                     /translation="MSTMNTVGRPTTPNAVQSKPATLTGGRGLLQNEHMIFESDGWGK
                     TGVDLPEPKTDGSDLGDLVRKDPIGLPGLSEP"
     gene            complement(240..1586)
                     /gene="gcvPA"
                     /locus_tag="VF686_00420"
     CDS             complement(240..1586)
                     /gene="gcvPA"
                     /locus_tag="VF686_00420"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX8660298.1"
                     /translation="MRYLPLTPDDRSAMLAAIGAKSIDDLFVDVPKAAHRDGFVDLPR
                     VAGELEVERALGAMAAKNASAGTVPFFCGAGAYKHHVPATVDHVIQRSEFLTSYTPYQ
                     PEIAQGTLQYLYEFQTQVANLTGMPVANASLYDGSTAMAEGVLMATRVTRRNKAVISG
                     GVHPHYVKATETVVHAVGVETQVLSAAVDAEADVIAGIGPDTACVVVQTPNVFGTATD
                     VTKIAEAAHAAGALLIVVVTEAVSMGLLKSPGEMGADIVAAEGQSIGNALNYGGPYVG
                     LFACREKLVRQMPGRLTGETVDADGERGFVLTLSTREQHIRRDKATSNICTNSGLCTL
                     AFTIHMSLLGETGLRRMALLNHEKALATRDALAAIPGVEVLTTRFFNEFAVKLPKPAA
                     EVVEALAAHRILAGVPYSRLAPEAGMDDVLLVAATEMTMDADIQILAGSLAKVLAN"
BASE COUNT          234 a          551 c          563 g          279 t
ORIGIN      
        1 gggctccgag agacccggca ggccgatcgg atccttgcgg accagatcgc cgaggtcgga
       61 tccgtcggtc ttcggttcgg gcaggtcgac gccggtcttg ccccagccgt cgctctcgaa
      121 gatcatgtgc tcgttctgca acagtccgcg gccgccggtc agggtggcgg gcttgctctg
      181 cacggcgttc ggtgtggtcg ggcggccgac agtgttcatg gtgctcatcg ttccgctcct
      241 cagttcgcca gaaccttggc gagcgagccc gccaggattt ggatgtccgc gtccatggtc
      301 atctcggtcg ccgcgaccag caggacgtcg tccatgccgg cctcgggcgc cagccggctg
      361 tagggcacgc cggcgaggat gcggtgcgcc gccagcgcct cgaccacctc ggccgccggc
      421 ttcggcagtt tcacggcgaa ctcgttgaag aagcgcgtgg tcagtacctc gacgccggga
      481 atggcggcga gggcgtcgcg ggtcgccagc gccttctcat ggttcagcag cgccatcctg
      541 cgcagccccg tctcgcccag caggctcatg tggatcgtga aggcgagggt gcacaggccc
      601 gagttggtgc agatgttcga cgtcgccttg tcccggcgga tgtgctgctc gcgcgtcgac
      661 agggtcagga cgaagccgcg ctcgccgtcg gcgtcgacgg tctcgccggt caggcggccg
      721 ggcatctgac gcaccagctt ttcgcggcag gcgaacagac cgacgtaggg gccgccatag
      781 ttcagcgcat ttccgatcga ctggccttcg gcggcgacga tgtcggcccc catctcgccc
      841 ggcgacttca gcaggcccat cgacacggcc tcggtcacca cgacgatcaa cagggcgccg
      901 gcggcgtggg ccgcctcggc gatcttcgtc acgtccgtcg ccgtgccgaa cacgttcggg
      961 gtctggacga ccacgcaggc ggtgtcagga ccgatcccgg cgatgacgtc ggcctcggcg
     1021 tcgacggcgg ctgacagaac ctgggtctct acgcccacgg cgtggaccac ggtctcggtc
     1081 gccttgacgt agtgcggatg caccccgccc gagatcaccg ccttgttgcg gcgggtcacc
     1141 cgtgtggcca tcagcacgcc ctcggccatg gcggtcgagc cgtcatagag gctggcgttg
     1201 gccaccggca tgccggtcag gttcgcgacc tgggtctgga actcgtacag atactgcagc
     1261 gtgccttggg cgatttcggg ctggtaaggc gtgtagctgg tcaggaactc cgaccgctgg
     1321 atcacgtgat ccaccgtcgc cgggacgtgg tgcttgtagg cgccggcccc gcagaagaac
     1381 gggaccgtgc ccgccgacgc gttcttcgca gccatggccc ccagcgcccg ctcgacctcc
     1441 agctcccccg ccacccgggg caggtcgacg aatccgtcgc gatgcgccgc cttgggcaca
     1501 tccacgaaca ggtcgtcgat ggatttggct ccaatggccg ccagcatggc ggatcggtcg
     1561 tcaggcgtca gggggaggta gcgcatgggc gtttcacctt gtcatcccgg ccgaagcgaa
     1621 gcggaga
//
LOCUS       DASVVN010000023         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_158818,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000023 DASVVN010000000
VERSION     DASVVN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158818"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VF686_00425"
     CDS             complement(<1..1367)
                     /locus_tag="VF686_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX8660299.1"
                     /translation="MTPRLAGGMQGLVVVLALVMTSPAEAQDGPRLAAIFDDHAVLQR
                     DRPISVWGTGAAGARLTVDLGGHTVETTTGADGLWSARLPARGAGGPFTLRIRDAAGA
                     GDQAADVLVGDVWLCSGQSNMELPVSRTLNGPSVTGSANDPALRLLTIAKDFDPVARN
                     EFRNPVAWAPAGPDTVGNFSATCYYFGQDLRRDHDVPMGLINASWGGSNIETWMSGAA
                     LERAGEGERLSILSRLTTDPLAAQAAWGDQWQAWWRGQASDTPWSPADPGPWSAVPAM
                     APWERWGVPELAAYNGMVWFRLDIELTAEQAREAQTLTLGNVDEADQTWVNGVAVGAS
                     GSGDRRYDLPRGLLKAGANTIVVNAYDTWEVGGLYGPAEKRALILANGEAVPLDPAGW
                     RYRVATNVTIAPPRAPWSSTSGLTTIGNAMIAPMHDYGLRGALWYQGESNTGEGLRYR
                     DLLAGL"
     gene            complement(1373..>3514)
                     /locus_tag="VF686_00430"
     CDS             complement(1373..>3514)
                     /locus_tag="VF686_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8660300.1"
                     /translation="AASAARPGARVPLIYGIDAVHGHNNIVGATVFPHNIGLGAARDP
                     DLIRRIGEATALEVAATGADWTFGPTLAVPRDDRWGRAYEGYAEDSEVQRSYAGPMTL
                     GLQGELTAGRPLSPGRIAGSAKHFLADGGTFEGVDQGDFRGTEQALIDTHLGGYVQAI
                     DAGVLSIMASFSGWNGVKHSGNPTLLIDVLRGPLGFDGFVVSDWNAHGQLPGCSNESC
                     ALAINAGIDMLMAPDSWKPLYASTLAQARSGEIPAARLDEAVRRILTVKVKAGLFETA
                     RPMEGRFEVLASPEHRTLAREAVRKSLVLLKNENAVLPIRAGARVLVAGAAADDIGQA
                     AGGWTLSWQGTGNTNADFPQGQSIRAGIEAAVREAGGTASFAADGSFSEKPDVAIVVF
                     GETPYAEFQGDLDNLDFAPGEPLEMLRRLKTAGIPTVAVFLSGRPMWTNPEINASDAF
                     VAAWLPGAEGGGIADVLIGDAGGQPRHDFTGRLSFSWPKDAVGAPLNRGEAGYDPQFA
                     YGYGLSYAAPAPVGALSEESGVSGEASVIDRYFVDGRFVGPWGLVLRDAGGELRLGGE
                     ARGESPRRGVTAAPADGEAQETARALTFANGGGEAAITGPAVDLGRQSNGELTIAFRY
                     RIDARPSAPVELVSGAGRVDISALFAAAPTGEWRTAAVRLSCLRDAGTNVMAVDQPWG
                     LATRGAFAITVEAIRLQPNNGDAVCSASTGR"
BASE COUNT          506 a         1307 c         1201 g          500 t
ORIGIN      
        1 agtccggcca gcaggtcgcg atagcggagg ccctcgccgg tgttggattc gccctggtac
       61 cagagcgcgc ccctcagccc gtagtcgtgc atgggggcga tcatggcgtt gccgatggtg
      121 gtcaggcccg aggtcgagga ccagggcgcg cgcggcgggg cgatggtcac attcgtcgcg
      181 acgcggtagc gccagcccgc cggatccagc ggaacggcct cgccgttcgc caggatcaac
      241 gcccgcttct cggcgggccc gtagaggccg ccgacctccc aggtgtcata ggcgttgacg
      301 acgatggtgt tggcccctgc cttgagcagg ccacgcggca gatcataacg acggtcgccg
      361 gatcccgagg ccccgacggc cacgccgttg acccaggtct ggtccgcctc gtcgacattg
      421 cccagcgtca gggtttgcgc ctcgcgcgcc tgctccgccg tcagctcgat atccagccgg
      481 aaccagacca tgccgttata ggcggccagt tcgggcacgc cccagcgctc ccacggggcc
      541 atggcgggga cggcggacca gggccccggg tcggcgggag accagggcgt gtcgctcgcc
      601 tgcccgcgcc accacgcctg ccactggtcg ccccaggccg cctgggccgc gagcggatcg
      661 gtcgtcagcc gcgacaggat ggacagccgc tcgccctccc cggcccgttc cagcgccgcg
      721 ccgctcatcc aggtctcgat gttggagccg ccccagctgg cattgatcag gcccatgggt
      781 acgtcgtggt cgcgccgcag gtcttggccg aaatagtagc aggtcgccga gaaattcccg
      841 accgtgtccg gccccgccgg agcccaggcg accggattgc ggaactcgtt ccgggccacc
      901 ggatcgaaat ccttggcaat ggtcagcagc cgcagggcgg ggtcgtttgc cgagccggtc
      961 acgctgggcc cattcaacgt ccggctgacc ggcagctcca tgttcgactg ccccgaacac
     1021 aaccagacgt cgccgaccag aacgtctgcg gcctgatccc ccgcgccggc ggcgtcgcgg
     1081 atcctcagcg tgaagggtcc gcccgccccg cgagccggaa ggcgggcgct ccacaggccg
     1141 tctgcaccgg tcgtggtttc gacggtgtgg ccgccgaggt cgaccgtcag ccgggcgccc
     1201 gccgcgcccg tcccccagac ggaaatcggg cggtcacgct gcagcacggc gtggtcgtcg
     1261 aagatggcgg cgaggcgcgg cccatcctga gcctcggccg gggatgtcat gaccagtgcc
     1321 agtacgacaa ccagaccctg cattcccccc gcgagccgcg gcgtcatcgt cctcatctcc
     1381 cggtgctcgc ggagcagacg gcgtcgccgt tgttgggctg cagccggatc gcctccacgg
     1441 tgatggcgaa ggcgccgcgc gtcgccagac cccagggctg gtcgactgcc atgacattgg
     1501 ttcccgcgtc gcgaagacag gacaggcgaa ccgcggcagt tcgccactcg cccgtcgggg
     1561 cggcggcgaa cagggcgctg atgtccacgc ggccggcgcc cgagaccagt tccaccggcg
     1621 ccgagggccg ggcgtcgatg cgatagcgga aggcgatggt cagttcgcca ttgctctgac
     1681 ggcccagatc caccgccggt ccggtgatgg cggcctctcc gcccccgttg gcgaaggtca
     1741 gggcgcgcgc cgtctcctgg gcctcgccgt cggctggggc ggcggtcacg ccgcgccggg
     1801 ggctctcccc gcgagcctcg ccgccgagac gcagctcgcc cccggcgtcg cgcagcacca
     1861 gaccccaggg cccgacgaac cggccgtcga cgaaatagcg gtcgatgacg gacgcctccc
     1921 ccgacacgcc gctctcttcc gacagcgctc ccaccggcgc cggcgccgca tagctgaggc
     1981 catagccata ggcgaactgc gggtcatatc ccgcctcgcc ccggttcagc ggcgcgccaa
     2041 cggcgtcctt gggccagctg aacgacagcc gaccggtgaa atcgtggcgc ggctgtcccc
     2101 cggcgtcgcc gatcagcacg tcggcgatcc cgccgccctc cgcgcccggc agccaggcgg
     2161 cgacgaaagc gtccgaggcg ttgatctcgg gattggtcca catcgggcgc ccggacagga
     2221 agacggcgac ggtcggtatg ccagccgtct tcagccgccg cagcatctcc agcggctcgc
     2281 cgggcgcgaa atcgagattg tccagatccc cttggaattc ggcgtagggc gtctcgccga
     2341 agacgacgat ggcgacatcc ggcttttcgc tgaacgaccc gtcggcggcg aagctggcgg
     2401 tcccgcccgc ctcgcgaacc gcggcctcga ttccggcgcg gatcgactgg ccctgcggga
     2461 agtcggcgtt ggtgttgccc gtgccctgcc agctcagggt ccagcccccc gccgcctgtc
     2521 cgatgtcgtc ggccgccgcg ccggccacca gcacccgcgc cccggcgcgg atgggcagga
     2581 ccgcattctc gttcttcagc agcaccagcg acttgcgcac ggcctcgcgc gccagggttc
     2641 ggtgttcggg cgaggccagc acctcgaacc ggccctccat gggacgggcg gtctcgaaca
     2701 ggccggcctt gaccttgacc gtcaggatgc ggcgcacggc ctcgtccagc cgggccgccg
     2761 gaatctcgcc cgaccgcgcc tgcgccagcg tgctggcgta gagcggcttc cagctgtccg
     2821 gcgccatcag catgtcgatg cctgcgttga tcgccagggc gcagctctcg ttcgaacagc
     2881 cgggcagctg gccatgggcg ttccagtccg agaccacgaa gccgtcgaag cccagcggcc
     2941 cgcgcagcac gtcgatcagc agcgtcggat tgcccgaatg cttgaccccg ttccagcccg
     3001 aaaaactggc catgatcgac agtacgcccg cgtcgatcgc ctggacgtag ccgcccagat
     3061 gggtgtcgat gagcgcctgc tccgtgcccc ggaaatcgcc ctggtccaca ccttcgaagg
     3121 tgccgccgtc ggcgaggaaa tgcttggccg agccggcgat ccggccgggg gacagcggcc
     3181 gcccggcggt cagttcgccc tgcaggccca gcgtcatcgg acccgcgtag gaccgctgca
     3241 cctcggagtc ctcggcatag ccctcatagg cccggcccca gcggtcgtcg cgcggcacgg
     3301 ccagcgtcgg tccgaacgtc cagtcggccc cggtcgccgc cacctccagc gctgtcgcct
     3361 ccccgatgcg gcggatcagg tccggatcgc gcgccgcgcc caggccgatg ttgtgaggaa
     3421 agaccgtcgc accaacgata ttgttgtgcc cgtgcacggc gtcgattccg taaatcagcg
     3481 gcacgcgcgc gccgggccgg gccgcggatg cggc
//
LOCUS       DASVVN010000024         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_924, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000024 DASVVN010000000
VERSION     DASVVN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VF686_00435"
     CDS             complement(<1..490)
                     /locus_tag="VF686_00435"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX8660301.1"
                     /translation="MSSTDTPKTGIGGAFRKAGHVLREDAARLGREIRAVRFRRPGKV
                     AVRWMASSFAVLTIALVIFFMLFQWNWLRGPIGRYASNKLDREVRITGDLDVKLFTRT
                     PRITADGITVRQPAWAGSGYMVEMKRLTVVVELFPLFKGDVRMPLLQVDQPNVILRRD
                     DEG"
     gene            complement(619..>1537)
                     /locus_tag="VF686_00440"
     CDS             complement(619..>1537)
                     /locus_tag="VF686_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8660302.1"
                     /translation="PIGLCTPGVTRTTANPTCGSPEGSVESWTLMANLLFDFMPAESA
                     FRPFVGFGVGINRVDMSVTGQFSNVGTITASNPRFQDLTINDEDLTFAYQGIAGVAWA
                     MTERLAVDLTYRYLGGAELDFDSRGSAENGLQPGRFSGEYADQSLTLGLRYAFSPPPA
                     PPPAPMPEPTPAPMPEPTPVPEPTPQPQPEAPRPVQREFVVYFPFDQSVLTPEAQTVV
                     QEAASYAQQGNATQVQVVGHADTSGSAAYNVRLSERRARAVADAMVGLGVNPGVITAD
                     WRGETQPAVQTGDGVREPLNRRATVNVNF"
BASE COUNT          220 a          513 c          580 g          224 t
ORIGIN      
        1 ggccctcgtc gtcgcggcgc aggatgacgt tcggctggtc cacctgcagc agcggcatgc
       61 gcacgtcgcc cttgaacagc gggaacagct ccacgaccac ggtgagccgc ttcatctcga
      121 ccatgtagcc gctgcccgcc caggcgggct ggcgcacggt gatgccgtcg gccgtgatcc
      181 tgggcgtgcg ggtgaacagc ttcacgtcga ggtcgccggt gatccgcacc tcgcgatcca
      241 gcttgttcga ggcgtagcgg ccgatcggcc cgcgtagcca gttccactgg aacagcatga
      301 agaagatgac cagggcgatg gtcagcaccg cgaagctgct ggccatccag cgcacggcga
      361 ccttgccggg gcggcggaag cgcacggcgc ggatctcgcg gccgaggcgg gcggcgtcct
      421 cacgcaggac gtggccggcc ttgcggaagg ctccgccgat gccggtcttg ggggtgtctg
      481 tcgagctcac gggggttcaa cgcatgaaac cgcccagtct gttcccgatc cctgctccgg
      541 cgggtgggcg gcggccccaa aggaaaaggg cccggctttc gccgggcccc ctccagaccg
      601 ctggtgcggt cggtacgctt agaagttgac gttgaccgtg gcgcgccggt tgagcggctc
      661 gcggacgccg tcgcccgtct gcacggccgg ctgcgtctcg ccccgccagt cggcggtgat
      721 cacgcccggg ttcacaccca ggccgaccat cgcgtcggcc accgcgcgag cgcggcgctc
      781 cgacaggcgc acgttgtagg cggccgaacc cgaggtgtcg gcgtggccca cgacctggac
      841 ctgggtcgcg ttgccctgct gggcgtagga cgcagcttcc tgcaccaccg tctgggcttc
      901 cggcgtcagg accgactggt cgaacgggaa gtagacgacg aactcgcgct gcaccgggcg
      961 cggcgcttcc ggctgcggct gaggcgtggg ctcaggcacc ggcgtgggct ccggcatggg
     1021 cgccggcgtg ggctcaggca tgggcgccgg cggcggggcc ggcggcggcg agaaggcgta
     1081 gcgcaggccc agcgtcagcg actggtcggc gtactcgccc gagaagcggc cgggctgcag
     1141 gccgttctcg gccgacccgc gcgagtcgaa gtcgagctcg gcgccgccga ggtaacggta
     1201 ggtcaggtcc accgccaggc gctcggtcat cgcccaggcg acgccggcga tgccctggta
     1261 ggcgaaggtc aggtcctcgt cgttgatggt gaggtcctgg aaccgcgggt tggaggcggt
     1321 gatcgtgccg acgttgctga actggccggt gaccgacatg tcgacgcggt tgatgccgac
     1381 gccgaagccc acgaacggcc ggaaggccga ttccgcgggc atgaaatcga acagcaggtt
     1441 ggccatcagg gtccaggact cgacggagcc ttccggcgag ccgcaggtcg ggttggccgt
     1501 ggtccgggtc acgcccgggg tgcacaggcc gatcggc
//
LOCUS       DASVVN010000025         5958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_27003, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000025 DASVVN010000000
VERSION     DASVVN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27003"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VF686_00445"
     CDS             complement(<1..529)
                     /locus_tag="VF686_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX8660303.1"
                     /translation="MTRLFPRFAIPFAVAAALALPAAAQQVRYYDVPPGGRAHDVAVT
                     PDGEIWWTVQRGGGHGILDPRTGETTIVPLGEGSAPHGVIVGPDGNAWITDGGQNAIV
                     RVDRRTREVRVWPLPADTGPVNMNTATFDHRGRIWFTGQTGFYGRLDPATDDMKVWAA
                     PRGRGPYGITTTPSGD"
     gene            complement(569..808)
                     /locus_tag="VF686_00450"
     CDS             complement(569..808)
                     /locus_tag="VF686_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX8660304.1"
                     /translation="MVQITYRIVEHDGGWAYQSGGTYSETYPTHDSARAAAIAVAREQ
                     KVPDEDALIEYADASGQWITERADGHDRPEVEVEG"
     gene            991..1401
                     /locus_tag="VF686_00455"
     CDS             991..1401
                     /locus_tag="VF686_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660305.1"
                     /translation="MEDLFRSYWWLLFPLAWFVFGGFNSFLNYRRQKDTLDLIKTYAA
                     KGQEPPEALLKVLERPIDADDDSWHSRRGDGSRMNDGNWFSVVLFGVMGAGFAYASYA
                     DIYGAGEAFLIVAFVLGALCLASLVSALMGRRRG"
     gene            1423..1962
                     /locus_tag="VF686_00460"
     CDS             1423..1962
                     /locus_tag="VF686_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8660306.1"
                     /translation="MDGFLTDAELVSRALTGSDAAFSRLVERHQAAVRGFLRRALGGG
                     WAEADDVAQDAFLAAWRSLRSLRDPAGVRAWLCGIAWRKAQDRIRSARRGAARDHVWL
                     ETADPPAGVSHEDRLSMAAAMADLPPDVRACVALCLADGWSNTEASVALDLPLGTVKS
                     HVARGRARLLKALGGPDDA"
     gene            1952..2248
                     /locus_tag="VF686_00465"
     CDS             1952..2248
                     /locus_tag="VF686_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660307.1"
                     /translation="MTPEQKLQALFATEAPPARDYAFQAQVAQRIALRRAWMTVAALV
                     PWGIAGMAILWALTPVIGPLGEDIAALIPAAGMLAGVAVSAGAALWLSRRFSAV"
     gene            complement(2235..3050)
                     /locus_tag="VF686_00470"
     CDS             complement(2235..3050)
                     /locus_tag="VF686_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8660308.1"
                     /translation="MIDRRILLAGLAAAPLTACAGTGVATEASGPSLTLVTFNIWHNQ
                     GDWAARLPLLIAAIRAENPDVIALQEVLEDAAVGLPNQARTIADALGGYEMHFVSTDA
                     EGAPRRYGNAILSRLPVLAHDWKKLEPLDDYRTAVRARVDVGGRAVDIVNTHLAWQAD
                     AQAVRARQLADLMAWLPKDGTPLIATGDFNAVQEDAGLAVLTGERFFSALPRGSAPTT
                     LNRAKGHPPRVIDHIFAEAARFAPVRAAVIGAEPTGGEYPSDHFGVAATIRLR"
     gene            3193..3600
                     /locus_tag="VF686_00475"
     CDS             3193..3600
                     /locus_tag="VF686_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX8660309.1"
                     /translation="MEDFIPLVAILSVFGCITAIVFGPNYLKFRERRETQETVRRAID
                     KGQDLPPEVLDALTKDVTKNLPSRTRDIRRGIIWLAVGIGIAAFGLINDMGWDGGDDW
                     NGNFGNGLLGIAAIPVTIGLAYLVLSFFNKNKD"
     gene            3626..4186
                     /locus_tag="VF686_00480"
     CDS             3626..4186
                     /locus_tag="VF686_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8660310.1"
                     /translation="MTQPLIDRHDVELAALAAAGGRREFGELVRRHGSAVRGLLRRMG
                     AQASLADDIAQDAFIQAFERCAEFRGEGTFAAWVKRIAARLYIKRVAKEARYIAESEQ
                     EDIAPAADQAGLMDLDEALKTLSEPERLCVSLCHGAGMAHPEIAAALNLPLGTVKSHV
                     KRGLDKLRARLQPQSAQPLGGTQHVG"
     gene            4176..4589
                     /locus_tag="VF686_00485"
     CDS             4176..4589
                     /locus_tag="VF686_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660311.1"
                     /translation="MSADEFDPQIERLFARTPHMPDAALFTAQVEQRLQKGSRVRFLA
                     LGLAGVIGGVVAVRETMNVNLGGGDGVVAGDALGQGIRSTSLSVQSAVQGGLDQFGLG
                     SLELGSMGGMQMFWIAAGALIALAAAGVMKLSQEM"
     gene            4665..5501
                     /gene="panB"
                     /locus_tag="VF686_00490"
     CDS             4665..5501
                     /gene="panB"
                     /locus_tag="VF686_00490"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX8660312.1"
                     /translation="MSSQKQETVRRLAAPDIRNRKGGEPIVCLTAYDAPTAALLDDHC
                     DLLLVGDSVGMVVHGMPNTVGVTLEMMILHGQAVMRGSKKAMVVIDMPFGSYEGGKEL
                     AYENCARVMKETGAQGVKLESGPTVAETIAYLVQRGIPVMGHVGLRPQAVLTDGAFKA
                     KGRTDEERLRVMSEAEATADAGAFSIVIEGVAEGLARDITAAVDKPTIGIGASSACDG
                     QILVTPDMLGLFDWTPKFVRRYADMRGEIDKAVAAYAADVKARRFPAEVETYFSKKPV
                     TP"
     gene            5594..>5958
                     /locus_tag="VF686_00495"
     CDS             5594..>5958
                     /locus_tag="VF686_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8660313.1"
                     /translation="MVDVFEQVEEELRSDRYKRLARTWLPVVGGVLLVALIAALAFWG
                     WDSWQTSKADKASVAYDRGMQSMQANNPVGADAAFTQAAKEGNGAYKALALMARAGIA
                     VDANRIPDALKLFDEAAKAS"
BASE COUNT          884 a         2039 c         2081 g          954 t
ORIGIN      
        1 cgtcgccgga gggcgtggtg gtgatcccgt aggggccccg cccgcgcggg gcggcccaga
       61 ccttcatatc gtccgtcgcc gggtccagcc ggccgtagaa gcccgtctgg ccggtgaacc
      121 agatgcgacc gcggtgatcg aaggtggcgg tgttcatgtt caccggtccg gtgtccgccg
      181 gcaggggcca gaccctgacc tcccgagtcc tccggtcgac acgcacgatc gcgttctggc
      241 cgccgtcggt gatccaggcg tttccgtcgg gcccgacgat gaccccgtgc ggggccgacc
      301 cttcgcccag cggcacgatc gtcgtctcgc ccgtccgcgg atcgaggatg ccgtggccgc
      361 cgccgcgctg gaccgtccac cagatctctc cgtccggggt gacggcgaca tcgtgggcgc
      421 ggccgccggg cggcacgtca tagtaccgga cctgttgcgc cgccgcgggc agggcgagtg
      481 cggccgccac cgcgaagggg atagcgaaac gggggaacaa gcgggtcatg gcgctctcct
      541 gcgttcgtta agggccgcaa gccctcgttc agccctcgac ttcgacctcc ggccggtcgt
      601 ggccgtcggc gcgctcggtg atccactggc cggaggcgtc ggcgtactcg atcagggcgt
      661 cctcgtccgg caccttctgc tcgcgcgcca cggcgatggc ggcggcccgc gccgagtcat
      721 gggtcggata ggtctcggaa taggtgccgc ccgactggta ggcccagcct ccgtcatgct
      781 cgacgatgcg ataggtgatc tggaccatgg gtgcgggctc cggtgagggc tgagtgtcgg
      841 ccagcgaacg ctaggccgca agatgggttg cgcggccggt cccgcggccg gcgcgcaatt
      901 gtttgccgcc gccgcatccg ctggcgcggt cggcggcgac taccctatga aagcgcgggc
      961 aaggcgtccg ccgaatgagg ggtgaagacc atggaagacc tgttccgttc gtactggtgg
     1021 ctgctgttcc cgctggcctg gttcgtgttc ggcggcttca acagcttcct gaactaccgt
     1081 cggcagaagg acacgctgga cctgatcaag acctatgccg ccaagggtca ggagcccccg
     1141 gaggccctgc tgaaggtgct ggagcgcccg atcgacgccg acgacgactc ctggcattcg
     1201 cggcggggcg acggcagccg tatgaacgac ggcaactggt tctcggtggt cctgttcggg
     1261 gtcatggggg ccggcttcgc ctacgccagc tatgccgaca tctacggcgc aggagaggcc
     1321 ttccttatcg tggccttcgt tctgggggcc ctgtgcctgg ccagtctcgt ctcggccctg
     1381 atgggacgcc ggcggggctg atgcccgacc gggaccgggg gcatggacgg gtttctgaca
     1441 gacgccgagc tggtgagccg cgcgctgacc ggctcggacg cggccttctc gcggctggtc
     1501 gaacggcatc aggcggccgt gcggggcttt ctgcgccgcg ccctgggcgg cggctgggcc
     1561 gaggcggacg atgtggcgca ggacgccttt ctggcggcgt ggcggtcgct gcggtcgctc
     1621 agggacccgg ccggcgtccg cgcctggctg tgcggcatcg cctggcgcaa ggcccaggac
     1681 cggatccgca gcgcgcgccg cggcgcggcc cgcgaccatg tctggctgga aacggccgac
     1741 ccgccggcgg gcgtgtcgca cgaggaccgg ctgtccatgg cggcggccat ggccgacctg
     1801 ccgccggacg tccgcgcctg cgtcgccctg tgtctggccg acggctggtc gaacaccgaa
     1861 gcctcggtcg ccctggactt gcctctcggc acggtgaagt cccatgttgc gcgcggtcgg
     1921 gcgcggttgc tcaaggcgct gggaggtccg gatgacgcct gaacagaaac tccaggccct
     1981 gttcgccacg gaagcgccgc cggcgcggga ctacgccttc caggcgcagg tcgcgcagcg
     2041 catcgccctg cgccgggcct ggatgacggt cgcggccctt gttccatggg ggatcgccgg
     2101 catggccatc ctctgggccc tgacccccgt gatcgggccg ttgggcgagg atatcgccgc
     2161 cctgatcccc gccgccggca tgctggcggg cgtggcggtc agcgccggcg ccgccctgtg
     2221 gctgtcgcgc cggttcagcg cagtctgatc gtcgccgcca cgccgaagtg atcggacgga
     2281 tattcgccgc cggtcggctc ggcgccgatc acggccgccc gaaccggcgc gaaccgggcc
     2341 gcctcggcga agatgtggtc gatgacccgc ggcggatgcc ccttggcccg gttcagcgtc
     2401 gtcggcgccg acccgcgcgg cagggcgctg aagaaccgtt cgcccgtcag caccgccagt
     2461 ccggcgtcct cctgcaccgc gttgaagtcg cccgtggcga tcagcggggt tccgtccttc
     2521 ggcagccaag ccatcagatc ggccagctgg cgcgcccgca cggcctgggc gtcggcctgc
     2581 caggccagat gggtgttgac gatgtcgacc gcccggccgc ccacgtcgac gcgcgcccgc
     2641 acggcggtgc gatagtcgtc cagcggctcc agcttcttcc agtcgtgcgc cagcaccggc
     2701 aggcggctca ggatcgcatt gccgtaccgg cggggagcgc cctcggcgtc ggtcgagacg
     2761 aaatgcatct catagccgcc cagcgcatcg gcgatggtgc gcgcctggtt gggcaagccg
     2821 accgcggcgt cctccagcac ctcctgcagg gcgatgacat cgggattctc cgcgcggatc
     2881 gcggcgatca gcagcggcag acgggcggcc cagtcgccct gattgtgcca gatgttgaag
     2941 gtgaccagcg tcagcgacgg ccctgacgcc tccgtcgcca cgcccgttcc ggcgcaggcg
     3001 gtcagcggcg cggcggccag cccggccaga aggatgcggc ggtcgatcat gggcgaggtc
     3061 tccggtcgtc ggtttctccg ccttacgaaa gtttcacctc gccgtccgca tccatgcggc
     3121 ctcttcacag cctcttgtcg tcagaaggcc gaggcatcac gctccggtct gacaagagaa
     3181 ggggatacac acatggaaga tttcattccg ctggtggcca ttctgtcggt cttcggctgc
     3241 atcaccgcca tcgtcttcgg cccgaattat ctgaagttcc gcgaacgccg cgaaacccag
     3301 gaaacggtcc gccgcgccat cgacaagggc caggacctgc cgcccgaagt gctcgacgcc
     3361 ctgaccaaag acgtcaccaa gaacctgccc tcgcggaccc gcgacatccg ccgcggcatc
     3421 atctggctgg ctgtcggcat cggcatcgcc gccttcggcc tgatcaacga catgggctgg
     3481 gacggcggcg acgactggaa cggcaatttc ggcaacggcc tgctgggcat cgcagccatc
     3541 ccggtcacca tcggcctggc ctatctggtc ctgagtttct tcaacaagaa caaggactga
     3601 gccgggtcgg acggggggac tgatgatgac ccaacctctg atcgaccggc acgacgtcga
     3661 gctcgccgcc ctcgcggcgg cgggcggacg gcgggagttc ggcgagctgg tgcgccgcca
     3721 cggttcggcg gtgcgcggcc tgctgcggcg gatgggggcg caagcctctc tcgccgacga
     3781 catcgcccag gacgccttca tccaggcgtt cgagcggtgc gccgagttcc gcggcgaggg
     3841 caccttcgcc gcctgggtca agcggatcgc ggcgcgcctc tacatcaagc gtgtcgcgaa
     3901 agaagctcgc tacatcgcgg aatccgaaca ggaagacatc gcgcccgccg ccgatcaggc
     3961 gggcctgatg gacctcgatg aagctctcaa gaccctcagc gagcccgaac gactgtgtgt
     4021 ctccctttgt cacggggccg gcatggcgca tcccgagatc gccgccgccc tgaatttgcc
     4081 actcggcacg gtgaagtctc atgtcaaacg tggtctggat aaactccgcg cgcggctaca
     4141 accgcaatcc gcgcagcccc tgggaggaac gcagcatgtc ggctgacgag ttcgatcccc
     4201 aaatcgagcg cctgttcgcg cgcacgccgc acatgcccga cgcggccctg ttcaccgcac
     4261 aggtcgagca gcgcctgcag aagggctcgc gcgtccgttt cctcgccctg ggcctcgccg
     4321 gcgtcatcgg cggcgtcgtg gcggtgcgcg agacgatgaa cgtgaatctg ggcggcggcg
     4381 acggcgtcgt cgcgggcgac gcgctggggc agggcatccg ctccacctcg ctcagcgtcc
     4441 agagcgcggt gcagggcggc ctcgaccagt tcggtctggg cagtctggaa ctggggtcca
     4501 tgggcggcat gcagatgttc tggatcgccg cgggggccct gatcgccctg gcggcggccg
     4561 gggtgatgaa gctgtcgcag gaaatgtgaa cccgtccccg ttgtccccag ggatgacggg
     4621 gaccggcctc atcgctatct aggtctccga acaggaggac cgccatgtcc agccagaagc
     4681 aggaaaccgt ccgccgtctc gctgcgccgg atatccgcaa ccgcaagggc ggcgagccca
     4741 tcgtctgtct gacggcctat gacgccccca ccgcggccct gctggatgat cactgcgacc
     4801 tgctgctggt cggcgacagc gtcggcatgg tggtgcacgg catgcccaac accgtcggcg
     4861 taaccctcga gatgatgatt ctccacggtc aggctgtcat gcgtggctcg aagaaggcca
     4921 tggtggtcat cgacatgccg ttcggcagct acgagggcgg caaggagctg gcctacgaga
     4981 actgcgcccg cgtgatgaag gagaccgggg cccagggcgt gaagctggaa agcggcccca
     5041 cggtcgcgga aaccatcgcc tatctggtcc agcgcggcat cccggtgatg gggcacgtcg
     5101 gcctgcggcc ccaggccgtg ctgacggacg gagccttcaa ggccaagggc cggaccgacg
     5161 aggaacgcct gcgcgtcatg tcggaggccg aggccaccgc tgacgccggg gcgttttcca
     5221 tcgtcatcga aggcgtcgcc gagggtctcg cccgcgacat caccgccgcg gtggacaagc
     5281 ctacgatcgg catcggggcc tcgtccgcct gcgacgggca gattctggtg acgccggaca
     5341 tgctgggcct gttcgactgg acgccgaaat tcgtccgtcg ctacgccgac atgcgcggcg
     5401 agatcgacaa ggcggtcgcc gcctatgccg cagacgtgaa ggctcgccgt tttcctgccg
     5461 aagtcgagac ctacttctcg aaaaagcccg ttacgcccta agggctgcgt tgcggcgggc
     5521 aggctgaccc tctagggtcc ggccggattg aaattcaacg gcctgagcgc cgttttccgg
     5581 gggcgacgta tccgtggttg atgttttcga gcaggtcgag gaggagcttc gctccgaccg
     5641 ctacaagcgg ctggcccgca cctggctgcc cgtcgtgggc ggcgtgcttc tggtcgcgct
     5701 gatcgccgcc ctggccttct ggggctggga tagctggcag acgtcgaagg cggacaaggc
     5761 ttccgtcgcc tacgaccgcg gcatgcaatc gatgcaggcc aacaatcctg tcggcgccga
     5821 cgccgccttc acccaggcgg ccaaggaagg caatggagcc tacaaggctc tggccctgat
     5881 ggcgcgcgcc ggcatcgctg tcgacgccaa ccggattccc gacgccctca aactcttcga
     5941 cgaggccgcc aaggcttc
//
LOCUS       DASVVN010000026         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_263948,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000026 DASVVN010000000
VERSION     DASVVN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263948"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF686_00500"
     CDS             <1..273
                     /locus_tag="VF686_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8660314.1"
                     /translation="GRPVVGVNAGGVKETVDDTVGQLAKSANPIQYAAAVEALFARDI
                     EAIGAAARVRTVERFAWNRVFEDLSSVYAEVSGEPAFAQPGEWKVH"
     gene            339..857
                     /locus_tag="VF686_00505"
     CDS             339..857
                     /locus_tag="VF686_00505"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX8660315.1"
                     /translation="MKRLLLAAAVSLLTTTSAMAQTVYLVRHGEKADASADPVLSEAG
                     QTRAAALAAALAEAHPGHIFTSPLQRTRLTAAPTAAFHSVTVEPIALDGGAAAHVAAI
                     AQRVHALPEDAVVLIVGHSNTVPLIARALGYAEAADMPECEYDRMTTLHLMGDTAAHG
                     QVTRYGAPTTCP"
     gene            complement(854..1681)
                     /locus_tag="VF686_00510"
     CDS             complement(854..1681)
                     /locus_tag="VF686_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660316.1"
                     /translation="MRFAAVFAALALGMASSVMAQSGAPAQGAAEALPPPVPIEEWPL
                     EKVSRIGAEMYRLDAAAWLATDALVKAISQEETRTIRGWLIAPVEDGLRVRFYREGSP
                     DPLPAWDVVIKGRVAGPVAPTPATVLAPEELAQARALATARANIGRLRCSPRPNTVIM
                     DDPDSDGWLVWLLTPMPENRTIPIGGHYRFRISADGGTVLQRDQLSNACFFADRPPAG
                     ARDAVLAYTQIVSRGPVETHVFLSIQNQLTLAIMAGDRYFSVGGARIADITDMVNRR"
     gene            complement(1714..>3685)
                     /gene="parE"
                     /locus_tag="VF686_00515"
     CDS             complement(1714..>3685)
                     /gene="parE"
                     /locus_tag="VF686_00515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX8660317.1"
                     /translation="APSTAAEPSSPPVAGNYSASSIEVLEGLEPVRKRPGMYIGGTDE
                     RALHHLFAEVLDNAMDEAVARHAKLITVDLDAEGWLSVRDDGRGIPVDPHPKHPGKSA
                     LEVVMTVLHSGGKFSGKAYETSGGLHGVGVSVVNALSEQLDVTVWRDGHEWKQSFSRG
                     LPLGSIVQGQPSKKKGTLIRFKPDEEIFGVGAAFKPARLFRMARSKAYLFRGVEIRWT
                     CAAERITDHTPTEAVFHFPNGLADALAERIGTLETVTPAFAGRVERKGEAGAVEWAVT
                     WSPIGFGEADGFVSSYCNTVSTPDGGTHEAGFRAALVKGLKAYGELINEKRANLITAE
                     DVIANAGAMISVFIRNPEFQGQTKDRLSSPDAQRLVEQLMRDPLDHWLTESPKQANTL
                     LGFVIDRAEDRLRRRKDKEVQRAQATRKLRLPGKLSDCSRQSANGTELFIVEGDSAGG
                     SAKQARDRTTQAILPLRGKILNVASATADKLRANIELSDLALALGVQPGSRFNIDDLR
                     YERIVIMTDADVDGAHIAALLITFFYRSMPETIRQGRVYMALPPLYRISAGPLSEYAR
                     DDAHRDELLATVFKGKKTEIGRFKGLGEMMASQLKETTMDPKKRTLARVTVPESEDEI
                     EDLVERLMGRKPEARYQFIQENARFVVADLDV"
BASE COUNT          582 a         1208 c         1282 g          613 t
ORIGIN      
        1 ggccggccgg ttgtcggggt caacgccggc ggggtgaagg agacggtcga cgacacggtc
       61 ggccagttgg cgaagagcgc caatccgatc cagtacgccg ccgccgtcga ggccctgttc
      121 gcccgcgata tcgaggccat cggcgccgcc gcccgggtgc ggacggtcga gcgtttcgcc
      181 tggaaccgcg tgttcgagga tctgagctcg gtctacgccg aggtcagcgg tgaaccggcc
      241 ttcgcccaac cgggcgagtg gaaggttcac tgaggcctgt ccactcgaca tgcggctcgc
      301 gcccggtcaa tctcgggcga aatcggggat ttgagatcat gaaacgcctc ctgctcgcgg
      361 ccgccgtctc gctgctgacc accacttcgg ccatggccca gaccgtctat ctggtgcggc
      421 atggtgagaa ggccgacgcc agcgccgacc cggtgctgtc ggaggccggg caaacccgcg
      481 cagccgcctt ggcggccgcg ctggcggaag cgcatcccgg ccacatcttc accagccccc
      541 tgcagcgcac gcgcctcacg gcggcgccga ccgccgcctt ccattcggtc acagtcgagc
      601 cgatcgcgct cgacggcggt gcggcggctc acgtggcggc aatcgcccaa cgcgtacacg
      661 ccctgccgga ggacgcggtc gtgctgatcg tcggccacag caacaccgtt cccttgatcg
      721 cgcgcgcgct gggttatgcc gaggcggccg atatgcccga gtgcgaatac gaccggatga
      781 cgacgctgca cctgatgggc gacacggccg cccacgggca ggtcacccgc tacggtgcgc
      841 cgacgacctg cccctagcgg cggttcacca tgtcagtgat gtcggcgatc ctcgcgccac
      901 cgactgaaaa gtagcggtcg cctgccatga tcgccagcgt cagctggttc tggatcgaca
      961 ggaagacgtg cgtctcgacc ggtccgcgag agacgatctg ggtgtaggcc agaaccgcgt
     1021 cacgcgcgcc cgcgggcgga cggtcggcga agaagcaggc gttgctgagc tggtcgcgct
     1081 gcagaaccgt gccgccgtcc gcgctgatgc ggaacctgta gtgtccgccg atcggaatgg
     1141 ttctgttttc cggcatgggc gtaagcagcc agaccagcca gccgtcgctg tctggatcgt
     1201 ccatgatgac ggtattgggg cgtggcgagc accgcagccg gccaatattg gctctcgccg
     1261 tcgccaaggc ccgcgcctgg gccagttcct ccggcgccag aacagtcgcc ggcgtcggcg
     1321 cgaccggacc cgcgacgcgg cccttgatca ccacgtccca ggccggaagg ggatccggac
     1381 ttccttcgcg atagaaacgg accctgagtc cgtcctcgac aggcgcgatc agccagccgc
     1441 ggatggtccg ggtttcttcc tgactgatcg ccttcaccag cgcatcggtc gccagccacg
     1501 cggcggcgtc gagacggtac atctctgcgc cgatgcggga gactttctcg agcggccatt
     1561 cctcgatcgg cacgggcgga ggaagggctt ccgcggcgcc ttgagcgggc gcaccggact
     1621 gggccatgac ggatgaggcc atcccgaggg ccagagcggc gaaaacggcg gcgaagcgca
     1681 tgaacaactc ccggtttcgg gagcaccttg ccatcacacg tccagatcag ccaccacgaa
     1741 ccgggcgttt tcctggatga actggtagcg ggcctcgggc ttgcggccca tcaggcgttc
     1801 gaccagatcc tcgatctcgt cctctgactc cggcaccgtg acccgcgcga gcgtgcgctt
     1861 cttcggatcc atggtggtct ccttgagctg ggaggccatc atctcgccca gacccttgaa
     1921 ccggccgatc tccgtcttct tgcccttgaa caccgtggcc agcagctcgt cgcggtgggc
     1981 gtcgtctcgg gcgtattccg acagcggtcc ggcgctgatc cggtagagcg ggggcagggc
     2041 catatagacc cggccctgac ggatcgtctc cggcatggag cggtagaaga aggtgatcag
     2101 cagggcggcg atgtgggcgc cgtccacgtc ggcgtcagtc atgatgacga tccgctcgta
     2161 acgcagatcg tcgatgttga accggctgcc cggctggacg cccagcgcca gcgccagatc
     2221 ggacagttcg atattggcgc gcagcttgtc ggccgtggcc gaggcgacgt tcaggatctt
     2281 gccgcgcagg ggcaggatgg cctgggtggt gcggtcccgc gcctgtttgg ccgagccgcc
     2341 ggccgagtcg ccctcgacga tgaacagttc ggtgccgttg gccgactggc gcgaacagtc
     2401 cgacagcttg ccgggcaggc gcagcttgcg cgtggcctgg gcgcgctgga cttccttgtc
     2461 cttgcgacgg cgcaggcggt cttcagcacg atcgataaca aagcccagca aggtgttggc
     2521 ctgcttggga ctttcggtca gccagtgatc cagcggatcg cgcatcagct gttcgaccag
     2581 acgctgggcg tcgggcgagg agaggcggtc cttggtctgg ccctggaact cgggattgcg
     2641 gatgaagacc gagatcatgg cgccggcgtt ggcgatgacg tcctcggccg tgatcagatt
     2701 ggcgcgcttc tcattgatca gctcgccata ggccttgagg cccttgacca gggcggcgcg
     2761 gaaaccggcc tcgtgcgtgc cgccgtcggg cgtggagacg gtgttgcagt aggaggagac
     2821 gaagccgtcg gcctcgccga agccgatcgg cgaccaggtc acggcccatt cgacggcccc
     2881 ggcctcgccc ttgcgttcga cgcggccggc gaaggccggg gtgaccgtct ccagcgtgcc
     2941 gatccgctcg gcgagggcgt cggccagtcc gttggggaag tggaagaccg cctcggtcgg
     3001 ggtgtggtcg gtgatccgtt cggcggcgca ggtccagcgg atctcgacgc cgcggaacag
     3061 ataggccttg gagcgggcca tgcggaacag acgggcgggt ttgaaggccg cgccgacgcc
     3121 gaagatttcc tcgtccggct tgaaccggat cagggtgccc ttcttcttcg acggctgccc
     3181 ctgaacaatc gatcccagcg gcaggccgcg actgaaggat tgcttccact catgcccgtc
     3241 gcgccagacg gtgacgtcca gctgttcgct caaggcgttg acgacgctga cgcccacgcc
     3301 atgcaaaccg ccagaggttt cataggcttt accggaaaac ttgccgcccg agtggaggac
     3361 ggtcatgacc acttccagcg ccgacttgcc cggatgcttg ggatggggat cgacggggat
     3421 gccgcgaccg tcgtcgcgga ccgacagcca gccctcggcg tcgaggtcca cggtgatcag
     3481 cttcgcatgg cgggccacag cctcgtccat ggcgttgtcg aggacttcag cgaacaggtg
     3541 gtgcagcgcc cgctcgtcgg tgccgccgat gtacatgccc gggcgtttgc ggacgggctc
     3601 aaggccttcc agcacctcga tcgaggaggc ggagtagttg ccggcgaccg gggggcttga
     3661 gggctccgcg gcggtcgagg gcgcg
//
LOCUS       DASVVN010000027         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_106419,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000027 DASVVN010000000
VERSION     DASVVN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106419"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..>1671)
                     /locus_tag="VF686_00520"
     CDS             complement(80..>1671)
                     /locus_tag="VF686_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX8660318.1"
                     /translation="TPLPARTALGTDADPIMLEVFNSRFMAAAEQMGEALRATAYSVN
                     IKERLDFSCAVFDATGALIANAPHIPVHLGSMGESIRTVIRSRSGAADGRGMLRGDVY
                     MLNAPYNGGTHLPDITVIMPVFLEAADAPAFFVAARGHHADVGGTTPGSMPPSSKTVE
                     DEGVLIDDFLLIDAGRLRDAEARALFASSRYPSRNVGQNMADLKAQVASCARGADELT
                     RMVGEFGRDVVTAYMSHIQDNAEEAVRRAIAALKPGAFELEMDDGAVIRVRIDVDAEA
                     RSAVVDFTGTSDQRPNNFNAPLSITRAATLYVFRTLVDDAIPLNEGCLKPIRLIVPEG
                     SMLNPRYPAAVVAGNVETSQAVVDCLYGALGVLAASQGTMNNFTFGDDARQYYETIAG
                     GAGAGPGFDGASAVQTHMTNSRLTDPEVLETRFPVLLEEFSIRRGSGGDGANRGGDGA
                     VRRVRFLEPLTAAILSNHRRTAPFGAAGGQDGATGINRIERTDGAREALASTAEVRMA
                     PGDVFVIETPGGGGYGPKKAS"
BASE COUNT          250 a          582 c          565 g          274 t
ORIGIN      
        1 ccagcacgac gaccgcgatc cccagaagca caagcatacg gattctcccc ttggcggatg
       61 gttacgcctg tcgcgccgtt caggaagcct ttttcggtcc gtagccgccc ccgcccggcg
      121 tctcgatgac gaagacgtcg cccggggcca tgcggacttc ggcggtggag gcgagggctt
      181 cgcgcgcgcc gtcggttcgt tcgatgcggt tgattccggt cgctccgtcc tgaccccccg
      241 ccgctccgaa cggcgccgtg cgacggtggt tggacaggat ggccgccgtc aggggttcga
      301 ggaagcgcac gcgccgcacc gcgccgtcgc cgccccggtt cgccccgtct ccgcccgagc
      361 cgcgccggat cgagaactct tccagcagga ccgggaaccg cgtctccagc acctcgggat
      421 cggtcagtcg gctgttggtc atatgggtct gcaccgccga ggcgccgtcg aagccggggc
      481 ccgcgccggc tccgcccgcg attgtctcgt aatactgccg ggcgtcgtcg ccgaaggtga
      541 agttgttcat cgtcccctgg ctggccgcca gcacgcccag cgcgccgtac aggcagtcga
      601 ccacggcctg gctggtctcg acattccccg cgacgacggc ggcgggatag cgggggttca
      661 gcatcgaccc ctccggcacg atcagccgga tcggcttcag acaaccctcg ttcagcggaa
      721 tggcgtcatc caccagcgtg cggaagacgt acagcgtcgc ggcgcgggtg atcgacagcg
      781 gcgcattgaa attgttcggc cgctggtcgc tggtcccggt gaagtcgacg accgcgctgc
      841 gggcctccgc atcgacatcg atccgcaccc tgataaccgc cccgtcgtcc atctccagtt
      901 cgaaggcgcc gggcttcagg gcggcgattg cgcgccggac cgcttcctcg gcattgtcct
      961 gaatgtggct catgtaggcg gtgacgacgt cgcgcccgaa ctcgccgacc atgcgtgtca
     1021 gttcgtcggc gccgcgtgcg caggaggcga cctgggcctt caggtcggcc atgttctgcc
     1081 cgacgttgcg cgacggatag cggcttgagg cgaacagggc gcgcgcctcg gcgtccctca
     1141 gccgtccggc gtcgatcagc aggaagtcgt cgatcagaac gccctcgtcc tcgaccgtct
     1201 tcgacgatgg cggcatcgat cccggcgtag tccccccgac atccgcatga tggcctcggg
     1261 ccgcgacgaa aaaggccggc gcgtcggcgg cctccagaaa gaccggcatg atgacggtga
     1321 tgtcgggcag gtgggtgccg ccattgtagg gcgcgttcag catgtagacg tcgccgcgca
     1381 gcatcccccg gccgtcggcc gcaccgctgc gcgaacggat caccgtgcgg atgctctcgc
     1441 ccatcgagcc cagatggacc gggatgtgcg gcgcattggc gatcagggcg cccgtcgcgt
     1501 cgaagacggc gcacgagaag tccagccgct ccttgatgtt caccgaatag gccgtggccc
     1561 gcagagcctc ccccatctgt tcggcggcgg ccatgaaacg gctgttgaac acctccagca
     1621 tgatcggatc ggcgtcggtt cccagcgccg tgcgggcggg caggggagtg a
//
LOCUS       DASVVN010000028         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_106476,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000028 DASVVN010000000
VERSION     DASVVN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106476"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF686_00525"
     CDS             <1..232
                     /locus_tag="VF686_00525"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX8660319.1"
                     /translation="DYLPSSLKAQAAAEPVFEELEGWSESTAGARSFRDLNANAVKYV
                     RRIEELIGAPVALLSTSPERDDTIMMHDPFRG"
     gene            319..1104
                     /locus_tag="VF686_00530"
     CDS             319..1104
                     /locus_tag="VF686_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8660320.1"
                     /translation="MLAKLAPTLRRVLIVDPNPHSARMLTDIIKGLGAGEIVVEPNTD
                     RALRTAAELEPGIIFTERTGDKLDGEALARNVRRSHLGCRRVPIIMVTAEATASTILG
                     ARDAGVHEFLRKPFTSGDLLKRVENVALKPRDWVEAVGYVGPDRRRFNSGEFSGPGKR
                     KADKPATGTAADAAAKDQAMRILASALDQFDSDPIQAVRAIREQASQLKALAMKASDS
                     RLVVAVGALEVSLASGPATKETLAAPIGTLLAMAPVEPVKRVG"
     gene            complement(1118..>2030)
                     /locus_tag="VF686_00535"
     CDS             complement(1118..>2030)
                     /locus_tag="VF686_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8660321.1"
                     /translation="ILAALAASVSLAACMASPAMVDPVETRTPITFSQLLNQPRQSAD
                     RRVPYGADPLQFGELWIPTKGRRHPVIVLIHGGCWRADLPGTELMDYMAADLRDRGYA
                     VWNLEYRRIGHLGGGYPGTFRDVAAGIDHLRALAPQYGLDLRRVAVSGHSAGGHLALW
                     AAGRDRLPATSPLRVDNPLPVRGVVALAGIADLAGYRESGPDACGGPPTIDGLVGVQG
                     SDGRDVFADTSPPSLLPLGDRQVVVSGALDHIVPPRFGQHYATAAASAGDPAISVVLE
                     GAGHFELIDPTSTVWPRILAGYADLLR"
BASE COUNT          360 a          666 c          707 g          297 t
ORIGIN      
        1 ggactatctg ccgtccagcc tgaaggccca agccgcagcc gagccggtgt tcgaggagct
       61 ggaaggctgg agcgaaagca ccgccggggc tcgcagcttc cgcgatctga acgcgaatgc
      121 ggtcaaatat gtccggcgca tcgaggaact gatcggcgcg cccgtggcgc tgttgtcgac
      181 gagtccggaa cgcgacgaca ccatcatgat gcatgacccc ttccggggct aagggcgttc
      241 aaccggcctt aaccgcctcc cgttaaggat gatgatcccg atgcggagga gtgcgtcttg
      301 tttgctgtcg atagtcgcgt gctggccaag ctggccccga cgctgcggcg agttctgatc
      361 gtcgatccca acccccattc cgcgcgaatg ctgaccgata tcatcaaggg gctgggcgca
      421 ggcgagatcg tggtggagcc gaacaccgac cgtgcgctgc gcacggcagc cgagctggag
      481 ccaggcatca tcttcacgga acggaccggc gacaagctgg acggagaggc gctggcgcgc
      541 aatgtgcgac ggtcgcatct gggatgccgc cgcgtaccga tcatcatggt cactgccgag
      601 gctacggcca gcaccattct gggtgcgcgc gacgccggcg tgcatgagtt tctacgcaaa
      661 cccttcacca gcggcgatct gctgaagcgc gtcgagaatg tcgcgctcaa gccgcgggac
      721 tgggtcgagg cggtcggcta cgtcggaccg gaccggcggc ggttcaattc cggcgagttt
      781 tccggtcccg gcaagcgcaa ggcggacaag cccgcgaccg gcacggccgc ggacgccgcg
      841 gccaaggatc aggccatgcg tattcttgca tcggcgctgg accagttcga cagcgatccg
      901 atccaggccg tccgcgccat ccgggagcag gcatcccagt tgaaggcgct ggccatgaag
      961 gcttcggact cacggcttgt ggtcgctgtt ggcgcgctgg aagtcagctt ggcatcgggc
     1021 ccggcgacga aggagacgct ggcggctccg atcgggacgc tgctggccat ggctccggtc
     1081 gaaccggtga agcgcgtggg ctaggtccgt gcccggctta ccgaagcaga tccgcatagc
     1141 ccgcgaggat gcgaggccag actgtcgagg tcgggtcgat caactcgaaa tgccccgcgc
     1201 cctccagcac caccgatatc gcgggatcgc ccgctgacgc cgccgctgtc gcatagtgct
     1261 gaccgaaacg cggcggcacg atgtggtcga gtgcgccgga caccaccacc tgccggtcgc
     1321 cgagcggcag cagggacggc ggcgaagtgt cggcgaagac gtcgcggccg tcgcttccct
     1381 gaacaccgac cagcccgtcg atggtcggcg gccccccaca ggcgtcaggc cctgactcgc
     1441 gatagccggc caagtcggcg atcccggcca atgcgaccac gccgcgcacc ggcagggggt
     1501 tgtcgacccg caggggactc gtggccggca ggcggtcgcg accggcggcc cacagcgcca
     1561 gatggccgcc ggcggaatgg cccgaaaccg cgacccggcg caggtccagt ccgtactgcg
     1621 gcgccagcgc acgcagatgg tcgataccgg cggcgacatc cctgaacgtc cccggataac
     1681 cgccacccag atgaccgata cggcgatatt ccagattcca gacggcgtag ccgcggtccc
     1741 gcaggtcggc ggccatgtaa tccatcagtt ccgtgcctgg cagatcggcg cgccagcagc
     1801 cgccgtgaat gaggacgatg acggggtggc ggcggccctt cgtcggaatc cacaactcgc
     1861 cgaactggag tggatccgct ccatagggca cgcggcggtc cgcgctctgg cgaggctggt
     1921 tcagcaactg actgaaggtg atcggggtcc gggtctcgac ggggtcgacc atcgccggcg
     1981 aggccatgca ggcggcgaga gatacggacg cggccaaggc ggcgaggatc
//
LOCUS       DASVVN010000029         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_211475,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000029 DASVVN010000000
VERSION     DASVVN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211475"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..411)
                     /locus_tag="VF686_00540"
     CDS             complement(55..411)
                     /locus_tag="VF686_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8660322.1"
                     /translation="MADVKTHTGSCRCGAVAFEVDTDLDGLLECNCSHCYRKGLVLNF
                     VSPDAFRLKADGAQTEYQFNTHKIRHFFCETCGVQSFARGTAPNGDAMVAVNIRTLTD
                     IEPFSWTAQRVDGASF"
     gene            complement(411..>2266)
                     /gene="dnaE"
                     /locus_tag="VF686_00545"
     CDS             complement(411..>2266)
                     /gene="dnaE"
                     /locus_tag="VF686_00545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8660323.1"
                     /translation="YQDPRSTIPASQFNMKWVEAAGLVKFDFLGLKTLTVLDRARQYL
                     ERRGAAPDWNGLPLDDPKTYELMASGQTVGVFQLESQGMRDNLRKMRCGSIEEITALI
                     SLYRPGPMEMIDTYIDRKFGRQQVDYLHPSLTEVLTETYGVIIYQEQVMKIAQVLAGY
                     SLGEADLLRRAMGKKKKEEMDFQRARFIAGASEKGVSEEQSGGIFDLVAKFAGYGFNK
                     SHAAAYALISFQTGWLKATHPVEFMAASMSLDLSNTDKLAVFYQDCRRFEVPVKSPDV
                     NRSSADFDVAYDDGVGAVLYALGAIRNVGLEAMKHLVEVRETGGRFTDIFDFLERVDP
                     RSVNKRALENLARAGAFDSFHSNRRQLFEQADTLMAYCQSVAAERASSQVSLFGGDQA
                     GAARPRLKPVEPWVGPEKLDQELAAVGFYLSGHPLEDMAPALKRKRVTFVAEATALAE
                     SGHEAFLMAGVVRRRQERASAKSGEKFAFVTFSAPTGEVECLFPPEQLRKCREVLEVG
                     ASIMVRVRAKSADGEVRFFGDDASRLDALIDDGAMGLRIHVSAKSADAEALRTRLEKA
                     KSPGKGGEVSLIASLDGRREVELKLPGRYRLDGALRGALKSAPGVVLLEDA"
BASE COUNT          368 a          747 c          767 g          384 t
ORIGIN      
        1 cgagcggctg tacgccgtcg acggaacgcc ccgggcagcc ttcgtaccgc cgccctagaa
       61 gctggccccg tccacccgct gggcggtcca gctgaagggc tcgatgtcgg tcagggtgcg
      121 gatgttgacg gccaccatcg cgtcgccatt cggggccgta ccgcgagcga aggactgcac
      181 gccgcaggtc tcgcagaaga agtggcggat cttgtgggtg ttgaactggt attccgtctg
      241 ggccccgtcc gccttcaggc ggaaggcgtc cggcgagacg aagttgagca ccagcccctt
      301 gcggtagcag tgcgagcagt tgcactcgag cagaccgtcc agatcggtgt cgacctcgaa
      361 cgccaccgcg ccgcagcggc acgatccggt atgggttttg acgtcagcca tcatgcatcc
      421 tccagcagca ccacgccggg cgccgatttg agcgcgccgc gcagggcgcc gtccagccgg
      481 tagcggcccg gcagtttcag ttcgacctcg cgacggccgt ccagtgaggc gatcagggac
      541 acctcgccgc ccttgcccgg cgacttcgcc ttctcgaggc gcgtcctcaa ggcctcggcg
      601 tcggcggatt tggccgaaac atggatgcgc aggcccatgg cgccgtcgtc gatcagggcg
      661 tccagacgcg aggcgtcgtc gccgaagaag cgcacctcgc cgtcggccga cttggcccgc
      721 acccgcacca tgatcgaggc cccgacctcc agcacctcgc ggcatttgcg cagctgttcg
      781 ggcgggaaca gacattcgac ctcgcccgtc ggagcggaga aggtgacgaa ggcgaatttc
      841 tcgcccgact tggcgctggc ccgctcctgt cgacggcgca ccacgccggc catcaggaag
      901 gcctcgtggc cgctttcggc cagggcggtg gcctcggcca cgaaggtcac gcgcttgcgc
      961 ttcagcgccg gggccatgtc ctccagcggg tggcccgaca ggtagaagcc gaccgcggcc
     1021 agttcctgat ccagcttttc cggcccgacc cagggctcga ccggcttgag gcgcgggcgc
     1081 gcggcgcccg cttggtcgcc gccgaacagg gagacctggg acgaggcgcg ttcggccgcg
     1141 acgctctggc aataggccat cagggtgtcg gcctgttcga acagctggcg gcggttggaa
     1201 tggaagctgt cgaaggcgcc tgcgcgggcg aggttttcca gcgcccgctt gttgacgctg
     1261 cgcgggtcga cgcgctcgag gaagtcgaag atgtcggtga accggccgcc ggtctcgcgc
     1321 acctcgacca gatgtttcat ggcctccagg ccgacattgc ggatggcgcc cagcgcataa
     1381 aggacggccc caacgccgtc atcgtaggcc acgtcgaaat cggccgagga gcggttcacg
     1441 tccggcgact tcaccggcac ctcgaagcgc cgacaatcct gatagaagac agcgagtttg
     1501 tcggtattgc tgagatcgag cgacatggac gcggccatga attcgaccgg gtgggtcgcc
     1561 ttcagccagc cggtctggaa gctgatcagg gcgtaggcgg cggcgtgcga cttgttgaag
     1621 ccatagccgg cgaacttggc caccagatcg aagatgccgc ccgactgttc ctcggagacg
     1681 cccttttccg acgcgcccgc gatgaagcgc gcgcgctgga agtccatctc ctccttcttc
     1741 ttcttgccca tggcgcggcg aagaaggtcg gcctcgccga gggagtagcc cgccaggacc
     1801 tgggcgatct tcatcacctg ctcctggtag atgatgacgc cataggtctc ggtcagcacc
     1861 tcggtcaggg aggggtgcag atagtccacc tgctgccggc cgaatttccg gtcgatatag
     1921 gtgtcgatca tctccatcgg cccgggccgg tagagggaga tcagggcggt gatctcctcg
     1981 atcgaaccgc agcgcatctt gcgcaagttg tcgcgcatgc cttgggattc tagctggaac
     2041 accccgacgg tctggcccga ggccatcagc tcataggttt tcgggtcgtc cagcggcagg
     2101 ccgttccagt cgggggcggc gccgcgccgt tccagatact ggcgcgcgcg gtccagcacg
     2161 gtcagggtct tgaggccgag gaagtcgaac ttcaccagcc ccgcggcctc gacccatttc
     2221 atgttgaact ggctggccgg gatggtcgag cgcgggtcct ggtaca
//
LOCUS       DASVVN010000030         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_500876,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000030 DASVVN010000000
VERSION     DASVVN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500876"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VF686_00550"
     CDS             complement(<1..1276)
                     /locus_tag="VF686_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX8660324.1"
                     /translation="MSALHPDLLTVDAEGAIPVRFVGPRGRIDTALQGWADANGFKGK
                     SGQMLFAPDATGGIAAVLVGAGETFDPMLARALPAKLPPGLYRLEAEPEDARRAALAF
                     LLGAYVFDRYKPRPDRDRVRLVAPDGLDAAAATRIASACALAREMVDTPAADMGPLQI
                     ETIAREIAEEAGAVLTVITGDALLEENYPAVHAVGRAAAPHRAPRVIEIGWNLDRADL
                     PLVALVGKGVVFDSGGLDIKPAAGMRNMKKDMGGSAHALALGRLVMQAGLPVRLVVLV
                     AAVENAISADAFRPGDILSSRKGLTIEIGNTDAEGRLILADILTRAGEHAPDLTLDFA
                     TLTGAARIALGPELPPLYTDDETLAADLLDAARAVGDPLWRMPLWPGYRAALETEIAD
                     LRNDSAAWAQAGSVTAALFLQRFAPATGAWAHM"
     gene            1370..>1621
                     /locus_tag="VF686_00555"
     CDS             1370..>1621
                     /locus_tag="VF686_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660325.1"
                     /translation="MSRTTASLAILVLISGAAGAAQARSAAPRQAAPAPPRPAATPRQ
                     PATAEVRAAYERSDALSRSVFWTDQADIDPRDPVAGVKAA"
BASE COUNT          221 a          573 c          610 g          217 t
ORIGIN      
        1 ccatatgggc ccaggcgccg gtggcggggg cgaaccgctg caggaacagg gcggcggtca
       61 ccgagcccgc ctgcgcccaa gcggcggagt cattgcgcag gtcggcgatc tcggtctcga
      121 gcgcggcgcg atagccgggc cagagcggca tccgccacag cggatcgccg accgcgcggg
      181 cggcgtccag caggtcggcg gcaagggttt cgtcgtcggt gtagaggggc ggcagttcgg
      241 gccccagggc tatgcgggcc gcgccggtca gggtggcgaa atccagcgtc agatccggcg
      301 catgctcgcc cgcgcgggtc agtatgtcgg cgaggatcag ccggccctcg gcgtcggtat
      361 tgccgatctc gatggtcagg cccttgcggc tggacaggat gtcgccgggg cggaaggcgt
      421 cggccgagat ggcgttctcg accgcggcga ccagcacgac cagccgcacg ggcaggcccg
      481 cctgcatgac cagccggccg agggcgaggg cgtgggccga gccgcccatg tccttcttca
      541 tattgcgcat gccggcggcg ggtttgatgt ccagcccgcc ggagtcgaac accaccccct
      601 tgccgaccag ggcgaccagc ggcaggtcgg cgcggtccag attccagccg atctctatga
      661 cgcgcggcgc gcggtgcggg gcggcggcgc ggccgacggc gtgaaccgcc ggatagttct
      721 cctccagcag ggcgtcgccg gtgatcacgg tcaggaccgc ccccgcctcc tcggcgatct
      781 cgcgggcgat ggtctcgatc tgcaggggtc ccatgtcggc ggcgggggtg tcgaccatct
      841 cgcgtgcgag ggcgcaggcc gaggcgatcc gcgtcgccgc tgctgcatcg aggccgtcgg
      901 gcgcgaccag ccgcacccgg tcgcggtcgg gccggggttt gtagcgatcg aagacatagg
      961 ccccgagcag gaaggccaag gcggcccggc gggcgtcttc cggctccgcc tcgagccggt
     1021 acaggcccgg cggcagtttc gccggcaggg cgcgggccaa catgggatcg aaggtctcgc
     1081 cggcgccgac cagaacggcg gcgattccgc cggtggcgtc gggcgcgaac agcatctggc
     1141 ccgacttgcc cttgaagccg ttggcgtccg cccagccttg cagcgccgtg tcgattcgcc
     1201 ccctgggacc cacgaaccgc accggaatcg caccctcggc gtcgacggtc aggaggtcgg
     1261 gatgcagggc ggacatgggc gacggccttt ccggccggcg gtggccgatc ttaaccaagg
     1321 attgacgtga agggccgagt ctggcccccg aaccatccgg agcgcctcca tgtcgcgcac
     1381 gacagcctct ctcgcaatcc tcgtcctgat ttcaggcgcc gccggcgcgg cgcaggcccg
     1441 gtcggctgcg ccccggcagg ccgcgcccgc tccgccacgg ccggccgcca caccgcgcca
     1501 gccggccacc gccgaggtgc gcgccgccta tgagcgctcg gacgcgctca gccggtcggt
     1561 gttctggacc gatcaggcgg acatcgatcc gcgcgatccc gtggccggcg tgaaggcggc
     1621 g
//
LOCUS       DASVVN010000031         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_132727,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000031 DASVVN010000000
VERSION     DASVVN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132727"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /gene="fliM"
                     /locus_tag="VF686_00560"
     CDS             <1..986
                     /gene="fliM"
                     /locus_tag="VF686_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX8660326.1"
                     /translation="ERVLNQDEIDSLLGFDLGDDDGSERSGIRAIINSALVSYERLPM
                     LEIVFDRLVRLMTTSLRNFTSDNVEVSLDNISSIRFGDYLNSIPLPAILAVFRAEELD
                     NYGLLTVDSNLIYSIVDVLLGGRRGTAALRIEGRPYTTIERVLVQRMVEVVLADARAA
                     FEPLTPVHFTLDRLETNPRFAAIARPANAAILVKLRIDMEDRGGRIELLLPYATLEPI
                     RKMLLQQFMGEKFGRDNIWEGHLATELWTTETEVRAVLDEQQLPLSQVLALKVGDTLM
                     LNATPDSDISVRCGSIPVTTGRMGRKGQHIAVRVEAPISIEAATSLTKGKR"
     gene            986..1528
                     /locus_tag="VF686_00565"
     CDS             986..1528
                     /locus_tag="VF686_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HEX8660327.1"
                     /translation="MTGMILDGVLMLLLVAALGYGVRLERKLTALRAGQQAFASAVTE
                     LNSAAGRAEAALANLRASGQETDLLHDRIIKAREVKTQLEALIARGPASPAEPAPARA
                     RPQPEPAPVAVVEDERALRMAALAERIQGMAPAAARAPAPVAGGNNVAAILGALTANQ
                     SAKQSLNRARRSLDEDLFAA"
     gene            1561..>2075
                     /locus_tag="VF686_00570"
     CDS             1561..>2075
                     /locus_tag="VF686_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660328.1"
                     /translation="MSKIPRLLPLIAIAIGGVVVVRAVGVAPGMFEGAKAWAEEAVPA
                     AAAAAPAAAVCALTPEQLAQQAGISPAELRILQSLSGRRTELDARDADLAAMLPLLAT
                     AEQKLDAKVAALEAVKAEVRVLLGQVSEQEKAENDRLVAVYSAMRPKEAARVFATLSD
                     DVRLPVAAAMRP"
BASE COUNT          323 a          762 c          685 g          305 t
ORIGIN      
        1 ccgaacgggt cctgaatcag gacgagatcg acagcctgct gggcttcgac ctgggcgatg
       61 acgacggctc cgagcgttcc gggattcgcg ccatcatcaa ctcggccttg gtgtcgtacg
      121 agcgcctgcc gatgctcgag atcgtcttcg accgcctcgt gcggctgatg acgacgtccc
      181 tgcgcaactt cacctcggac aacgtcgagg tcagcctcga caacatctcg tcgatccggt
      241 tcggcgacta tctgaactcc atccccctgc cggccatcct ggcggtgttc cgggccgagg
      301 aactggacaa ctacggcctg ctgacggtcg actccaacct gatctattcg atcgtcgacg
      361 tgctgctggg cggccgccgg ggaaccgcgg ccctgcgcat cgagggccgg ccctacacca
      421 ccatcgagcg cgtgctggtc cagcgcatgg tcgaggtcgt gctggctgac gcccgcgccg
      481 ccttcgagcc gctgaccccg gtccatttca ccctcgatcg gctggaaacc aacccgcggt
      541 tcgccgccat cgcccgcccg gccaacgccg ccatcctggt caagctgcgc atcgacatgg
      601 aagaccgggg cggccggatc gaactgctgc tgccctacgc cacgctcgaa ccgatccgga
      661 agatgctgct gcagcagttc atgggtgaga agttcggccg cgacaacatc tgggaaggcc
      721 acctcgccac cgagctctgg accaccgaga ccgaggtccg ggcggtgctg gacgagcagc
      781 agctgccgct gtcgcaggtg ctggcgctga aggtcggcga caccctgatg ctgaacgcga
      841 cgcccgacag cgacatctcg gtccgctgcg gatcgatccc ggtcaccacc ggccggatgg
      901 gccgcaaggg ccagcatatc gcggtgcgcg tcgaggctcc gatcagcatc gaggccgcta
      961 ccagtctgac gaagggaaaa cgctgatgac cggcatgatt ctggacggcg tgctgatgct
     1021 gctgctggtg gccgcgctcg gctacggcgt gcggctggag aggaagctga cggccctgcg
     1081 cgccgggcag caggcctttg cgtccgctgt caccgaactg aacagcgccg ccggccgcgc
     1141 cgaggcggcc ctggccaatc tgcgggcctc gggtcaggaa acggatctgc tgcacgaccg
     1201 catcatcaag gcccgcgagg tgaagaccca gctggaggcg ctgatcgcgc gcggcccggc
     1261 atccccggcc gagccggcgc ccgctcgcgc cagaccccaa ccggagccgg ccccggtggc
     1321 cgtggtcgag gacgagcgcg ccctgcgcat ggcggccctg gccgaacgca tccagggcat
     1381 ggcccccgcc gccgcccgcg ctccggcgcc cgtcgccggt ggcaacaacg tggccgccat
     1441 cctcggggcc ctgaccgcta accagtctgc gaagcaaagc ctcaaccgcg ctcgccgtag
     1501 tctggacgaa gacctgttcg ccgcctgaca cccctttttt cgcccgctga gtaaccgatc
     1561 atgagcaaga tcccccgcct gctgcccctg atcgccatcg ccatcggcgg ggtggtcgtc
     1621 gttcgtgcgg tcggcgtggc ccccggcatg ttcgagggcg ccaaggcctg ggccgaggaa
     1681 gccgtgccgg ccgcggccgc cgcggcgccc gccgccgcag tctgcgccct gacgcccgaa
     1741 caactggccc agcaggcggg catctccccc gccgagctgc gcatcctgca gtcgctgtcc
     1801 ggacgccgca ccgaactgga cgcccgggac gccgatctgg ccgccatgct gccgctgctg
     1861 gccaccgccg aacaaaagct cgacgccaag gtcgccgccc tcgaggccgt caaggccgag
     1921 gtgcgggtgc tgctgggtca ggtcagcgag caggagaagg ccgagaacga ccggctggtc
     1981 gcggtctatt ccgccatgcg gcccaaggag gcggcccgcg tgttcgccac cctgtccgac
     2041 gatgttcgcc tgccggtcgc cgccgccatg cggcc
//
LOCUS       DASVVN010000032         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_53704, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000032 DASVVN010000000
VERSION     DASVVN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53704"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VF686_00575"
     CDS             <1..423
                     /locus_tag="VF686_00575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8660329.1"
                     /translation="DPASHSFNGLTPRNAVMFGPPAHIYVYRIYGLHWCLNFTCGGGA
                     AVLIRALEPTAGIDIMSARRGLESPASLCSGPGKLCQALGIDGSLNGGPIEAAVVLVR
                     GGASPIHVGPRIGITRGRDTPWRFSHGGSPYLSRRSTP"
     gene            complement(401..979)
                     /locus_tag="VF686_00580"
     CDS             complement(401..979)
                     /locus_tag="VF686_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8660330.1"
                     /translation="MKPLLASAACIVLLAACNERGASPAVDKAQDMAAAPVGQASAAT
                     LGANTVDAYVPNAAMADMYEIMAADIALERSQNAEVKELATMIKTDHTDASNKFKAIL
                     QGMSPPMTPPAALDQRRQGLIDNLRSAGAADFDRVYLDQQIAAHNEALTLHRGFSDNT
                     DAAPLAAHARSVVPKIEAHLRTAEQLKASNAG"
     gene            complement(979..1245)
                     /locus_tag="VF686_00585"
     CDS             complement(979..1245)
                     /locus_tag="VF686_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660331.1"
                     /translation="MPQYELRYLCGRDIHEIVDVPDPDEAEVLARRRLLFTDPGFAIA
                     ILYEGVELIRVTQRSKASAPRFRRPDKQARAGLSPSTKPTEHPR"
BASE COUNT          242 a          460 c          548 g          274 t
ORIGIN      
        1 gaccccgctt cgcacagctt caacggactg acgccgcgca acgccgtcat gttcggccca
       61 ccggctcata tctacgtcta tcgaatctac gggcttcact ggtgcctgaa cttcacctgc
      121 ggcggcggcg cggccgtgct gatccgcgcc ctggagccga cggccggcat cgacatcatg
      181 tcggctcgac gagggctgga aagcccggcg agcctttgct ccggcccggg caagctctgt
      241 caggccctcg gcattgacgg cagcctgaat ggcgggccga tcgaggcggc cgtcgttctg
      301 gtgcggggcg gcgccagccc gatacacgtc gggccgcgga tcggcatcac ccggggcaga
      361 gacaccccat ggcggttcag ccatgggggt tccccctacc tcagccggcg ttcgacgcct
      421 tgagctgttc ggccgtccgc aggtgcgctt cgatcttggg cacgaccgag cgggcgtggg
      481 ccgccagcgg agcggcgtcc gtgttgtcgg agaacccgcg gtgcagggtc agggcctcgt
      541 tgtgcgcggc gatctgctga tcgagataga cccgatcgaa atcggcggcg ccggccgagc
      601 gcaggttgtc gatcagaccc tggcgccgct gatcgagagc ggcaggagga gtcatgggcg
      661 ggctcatgcc ctgaagaatg gccttgaact tgttggaggc gtcggtgtgg tcggtcttga
      721 tcatggtcgc cagctctttg acctcggcgt tttgcgagcg ttcgagggcg atgtcggcgg
      781 ccatgatctc atacatatcg gccatggcgg cgttgggcac ataggcgtcg acggtattag
      841 cgccgagtgt cgcggcggat gcctgaccca cgggggcggc ggccatatcc tgcgccttgt
      901 cgaccgcggg cgaggccccg cgctcgttgc aggcggcgag caggacgata caggcggcgc
      961 ttgcgagaag aggtttcatc atcggggatg ctccgtgggt ttggtcgaag gtgaaagccc
     1021 ggcgcgggcc tgtttgtcgg gccggcgaaa tcgcggtgcc gacgccttgg atcgctgggt
     1081 gacgcggatc aattcgacgc cttcgtacag gatggcgatg gcgaagccgg gatcggtaaa
     1141 cagcagccga cggcgggcca gaacttcggc ctcgtcaggg tcgggaacgt ccacgatttc
     1201 gtggatgtcc cggccacaga ggtatctgag ttcgtactgg ggcattggtc ccttgcggcg
     1261 caggtcggct cgtttggaaa cccacgggac atcccgccgg ttcctgtgtc cggaagccga
     1321 cgaaaccgac caccgtggaa accgttagag cggcgcgacg ctcaggcgta gaccgggaag
     1381 ggcacttgaa atcgacaccg atcagtccgc cggttgaacg aggcgcgcgc ggccgcgacc
     1441 ttccggccta tgtggcgaac ggtctgatcg gattgagggt gcgggagaac ccgttcgatt
     1501 cgggcatatg catcgtcggc ggtt
//
LOCUS       DASVVN010000033         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_1210, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000033 DASVVN010000000
VERSION     DASVVN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1206)
                     /gene="tdh"
                     /locus_tag="VF686_00590"
     CDS             complement(154..1206)
                     /gene="tdh"
                     /locus_tag="VF686_00590"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX8660332.1"
                     /translation="MADTMKALAKTRPEVGLELIDAPIPVAGPEDVLIRVKRTAVCGT
                     DIHIWNWDEWSQKNVPVPMITGHEFSGEIVAIGKDVDRRLKVGQRVSAEGHVIDLNSE
                     AARAGHFHLDPDTRGIGVNRQGAFAEFVVAPAFNVIELPDDVPYEIGSILDPFGNAVH
                     TAQQFDLLGEDVLVTGAGPIGMMAAAVARHAGARTVVLTDINDFRLELAQKVAPGVRT
                     VNTTREDLKDVMHELGLKVGFDVALEMSGSPIAFKQCVDTLIMGGGMAMLGIPSKPME
                     TDWGAIILKALTIKGVYGREMFTTWRKMLGLLKAGLDLTPLITHQMSYERFEEGFEAM
                     RSGQSGKVVLNWDKAA"
     gene            complement(1206..2399)
                     /locus_tag="VF686_00595"
     CDS             complement(1206..2399)
                     /locus_tag="VF686_00595"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX8660333.1"
                     /translation="MTTATFYDRVRAELADIDAQGLTKPERIIASRQGPVIKVGGRDV
                     LNFCANNYLGLAGDQRVVQAGIRAQEQWGAGTASVRFICGTLEIHKELERSVADYLGF
                     EDTILFAAAFDANGGIFEPLFGEEDAIVSDSLNHASIIDGVRLCKAKRYRFLTSDMDD
                     LEAKLKEARAAGARNIVIATDGAFSMDGYIAKLNDIRALADRYDALIMVDDCHATGFL
                     GPQGRGSYAYHGVEIDFLTGTFGKALGGAMGGFICAKAEVVSLLKQRARPYLFSNALA
                     PAVCGSSLEAIRIAGGEEGDALRTQLFANAARYRAAMTEAGFNLLPGEHPIIPVMLGD
                     ARLAQDFAARALELGVYVIGFSFPVVPRGQARIRTQMSAAHSFEQIDQTVAAFTTAGR
                     ELGVI"
BASE COUNT          406 a          800 c          856 g          436 t
ORIGIN      
        1 gcagcgggaa cagccactgg atgaagtcgt ggttccgctc cagcgccgcg ttgtccatgg
       61 ccagaacgtc gaacagggtc cggccgcgcg cgtccgggcc ctcgccttcc agaaaggccg
      121 ttatcgcgcc ggagccggac atggggctgg ctcttaggcc gccttgtccc agttcagaac
      181 caccttgccg gactggcccg aacgcatggc ctcgaagcct tcttcgaaac gctcgtagct
      241 catctggtgg gtgatgaggg gggtcaggtc gaggccggcc ttcagcaggc ccagcatctt
      301 gcgccaggtc gtgaacatct cgcgcccgta gacgcctttt atggtcaggg ccttcaggat
      361 gatggcgccc cagtcggtct ccatcggctt cgaaggaatg cccagcatgg ccatgccgcc
      421 gcccatgatc agggtgtcga cgcattgttt gaaggcgatc ggcgaaccgg acatctccag
      481 cgccacgtcg aagccgacct tcaggccgag ctcgtgcatg acgtctttca ggtcttcgcg
      541 ggtggtgttc acagtgcgca cgccgggggc gaccttctgc gccagctcga ggcggaagtc
      601 gttaatgtcg gtcagaacga cggtgcgggc tccggcgtgg cgggcgacgg cggcggccat
      661 catgccgatg gggcccgcgc cggtgaccag cacgtcctcg cccagcagat cgaactgctg
      721 cgccgtgtgc acggcgttgc cgaacgggtc caggatcgaa ccgatctcgt agggcacgtc
      781 atccggcagt tcgatgacgt tgaaggccgg ggccaccacg aactcggcga aggcgccctg
      841 ccggttcacg ccgatgccgc gggtgtcggg atcgaggtgg aagtggcccg cccgggccgc
      901 ttccgagttc aggtcgatga catgcccttc agcggagacg cgctggccaa ccttcagccg
      961 gcggtcgacg tccttgccga tggcgacgat ctcgcccgag aactcgtggc ccgtgatcat
     1021 cggaacggga acgttcttct ggctccattc gtcccaattc cagatgtgga tgtcggtgcc
     1081 gcagacggcc gtacgcttga ccctgatcag cacgtcctcg ggcccggcga ccgggatcgg
     1141 agcgtcgatc agctcaagcc cgacttcggg ccgggtcttg gccagggcct tcatggtgtc
     1201 cgccatcaga taactcccaa ttcgcgaccg gccgtcgtga acgccgcgac cgtctgatca
     1261 atctgttcga agctatgggc cgccgacatc tgggtgcgga tgcgcgcctg cccccggggc
     1321 acgaccggga agctgaagcc gatgacatag acgccgagct cgagcgcgcg ggcggcgaag
     1381 tcctgcgcca gtctggcgtc gcccagcatc accggaatga ttggatgctc ccccggcagc
     1441 aggttgaagc ccgcctccgt catggcggcg cgatagcggg ccgcgttggc gaacagttgc
     1501 gtgcgcaggg cgtcgccctc ctccccgccg gcgatgcgga tggcttccag gctggagccg
     1561 cagacggccg gagcgagggc gttggagaag agatagggcc gggcgcgctg cttgagcagg
     1621 gacacgacct cggccttcgc gcagatgaag ccgcccatgg cgccgcccag cgccttgccg
     1681 aaggtgccgg tgaggaagtc gatctcgacg ccgtggtaag cgtaggagcc ccgcccctgc
     1741 ggcccgagga agccggtggc gtggcagtcg tcgaccatga tcagggcgtc gtaccggtcg
     1801 gccagcgccc ggatgtcgtt cagcttcgcg atatagccgt ccatcgagaa ggcgccgtcg
     1861 gtggcgatga cgatgttgcg ggctccggcg gctcgggcct ccttcagctt cgcctcgagg
     1921 tcgtccatgt ccgacgtcag gaaccggtag cgtttggcct tgcacagccg cacgccgtcg
     1981 atgatcgagg cgtggttcag gctgtccgag acgatggcgt cctcttcccc gaacagcggc
     2041 tcgaagatgc cgccgttggc gtcgaaggcg gcggcgaaca ggatggtgtc ctcgaaaccg
     2101 agatagtcgg cgaccgaccg ttccagctcc ttgtggattt ccagggtgcc gcagatgaaa
     2161 cggaccgagg ccgtgcccgc gccccactgc tcctgggccc tgattcccgc ctgaacgacg
     2221 cgttgatcgc cagcgaggcc gagatagttg ttggcgcaga agttcagaac atcgcgcccg
     2281 ccgaccttga ttaccggccc ctgtcgggag gcgatgatgc gctcaggctt ggtcagcccc
     2341 tgggcgtcga tgtcggcgag ttccgcgcgg acgcggtcat agaaggtcgc tgtcgtcatg
     2401 ggcgctccgc taggccaatt tcgtggctgc ttgaagcccg gaaatgatcg gggcggtttg
     2461 gcagggtttt gcaaaaacgg ggcgccaacc gtgccaaa
//
LOCUS       DASVVN010000034         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_238055,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000034 DASVVN010000000
VERSION     DASVVN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238055"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..178
                     /locus_tag="VF686_00600"
     CDS             50..178
                     /locus_tag="VF686_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8660334.1"
                     /translation="MQEVTASNGSTVYRTTVTSLSREQATGLCNAIKAAGGDCFVR"
     gene            186..1262
                     /gene="nagZ"
                     /locus_tag="VF686_00605"
     CDS             186..1262
                     /gene="nagZ"
                     /locus_tag="VF686_00605"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX8660335.1"
                     /translation="MTSAAIYGCSGHRLTEAEKAFFADARPWGFILFRRNVDSPEQMR
                     ALTGELRDAIGDPLCPILIDQEGGRVQRMGPPHWRKYPPGEAYLKAVNDPLAARELAR
                     LGARLIAHDLRAVGVNVDCAPVLDVPVPGAHDIIGDRAYAHDPATVAQLGRAAAEGFL
                     AGGVLPVIKHMPGHGRAFADSHQDLPVVHADFETLDGWDFAPFKALSDMPLAMTAHVV
                     FDAIDPKRPATTSKKAVGLMRDHLGFSGLIMTDDLSMKALSGSLRERAEASLKAGCDV
                     VLHCNGDLDEMRMVAEGTGKLKGRAAKRAEAAMARIVHTPEPLDEPEARARFAALLEG
                     RLDAARGPDVGEAQAPASSSATAR"
     gene            1272..2093
                     /locus_tag="VF686_00610"
     CDS             1272..2093
                     /locus_tag="VF686_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX8660336.1"
                     /translation="MSDTFQPNLDFTAAEQVDEREAFVVDLEGYEGPLHVLLALARTQ
                     KVDLLKLSITKLAEQYLAFVHEARRRNFSLAADYLVMASWLAYLKSRLLLPRTDKKGD
                     EPPAEEMAAALAFRLQKLEAMRKAVEAITARPRLKRDVFTRGDPEATVIVPSDQIDAS
                     LYELMAAYVTQRRREGLRQYNPGQRVEAFGLEAARDWLRDVLPRLEQWTPLEEVAPTR
                     SSHAEGPTQASFTASTLSASLELVKEGAMNVRQAEAFADLYLKRRGRGQPLELVP"
BASE COUNT          337 a          737 c          744 g          323 t
ORIGIN      
        1 cgccatcgtc gcccgctatc cgcagttcac ccgcggcgcc gacaagcggg tgcaggaggt
       61 gacggcgtcc aacggctcga ccgtctatcg caccaccgtc acgagcctga gccgcgaaca
      121 ggcgaccggc ctctgcaatg cgatcaaggc cgccggcggc gattgcttcg tgcgttgacg
      181 caggcgtgac ctccgccgcg atctacggct gcagcggcca tcggctgacg gaggcggaga
      241 aggccttctt cgccgacgcc cggccgtggg gcttcatcct gttccggcgc aatgtcgaca
      301 gccccgagca gatgcgggcg ctgacgggcg agctgaggga cgccattggc gaccccctct
      361 gcccgatcct gatcgatcag gaaggcgggc gggttcagcg gatggggccg ccgcactggc
      421 ggaaatatcc gccgggcgaa gcgtatctca aggccgtcaa cgatccgctg gcggcgcgcg
      481 aactggcccg tctgggtgcg cgtttgatcg cccatgatct gcgagcggtc ggcgtcaacg
      541 tcgactgcgc cccggtgctg gacgtgccgg tgcccggcgc ccacgacatc atcggcgacc
      601 gcgcttacgc ccatgacccc gccactgtag ctcagctggg ccgggcggcg gccgagggct
      661 tcctcgccgg cggcgtcctg ccggtgatca aacacatgcc gggccacggc cgcgccttcg
      721 ccgacagcca tcaggatctg ccggtggtcc atgcggattt cgagacgctg gacggctggg
      781 acttcgcccc gttcaaggcc ctgtccgaca tgccgctggc catgaccgcc catgtggtgt
      841 tcgacgccat cgacccgaaa cgcccggcca cgacttcaaa gaaggcggtc ggcctgatgc
      901 gcgaccatct cggcttcagc ggcctgatca tgaccgacga cctgtcgatg aaggccctgt
      961 ccggaagctt gcgcgaacgg gccgaggcct cgctgaaggc gggctgcgac gtggtcctgc
     1021 actgcaacgg cgatctggac gagatgcgga tggtcgccga ggggaccggc aagctgaagg
     1081 ggcgggcggc caaacgcgcc gaggccgcca tggcccgcat cgtccacacg cctgaaccgc
     1141 tggacgagcc cgaggctcgg gcccggttcg cggccctgct ggaaggacgg ctggacgccg
     1201 cgcgcggccc ggatgtcggc gaggcgcagg ccccggcctc aagcagcgcg accgcgcggt
     1261 aggccagaac agtgtccgac accttccagc ccaatctgga cttcaccgcc gccgaacagg
     1321 tcgacgagcg cgaggcgttc gtggtcgatc tggaagggta tgagggtccg ctgcacgtcc
     1381 tgctggcttt ggcgcggacg cagaaggtcg atctgctgaa gctgtcgatc accaagctgg
     1441 cggaacagta tctggctttc gtccacgagg cccggcggcg caacttctcg ctggcggcgg
     1501 actatctcgt gatggcgtcc tggctcgcct atctgaagtc gcgcctgctg ctgccgcgta
     1561 cggacaagaa gggcgacgag ccgccggccg aggaaatggc cgccgccttg gccttccggc
     1621 tgcagaagct ggaggccatg cgcaaggcgg tcgaggccat caccgcccgg ccccggctga
     1681 agcgcgacgt cttcacccgc ggcgaccccg aggcgacggt gatcgtgcct tccgaccaga
     1741 tcgacgccag cctgtacgaa ctgatggccg cctatgtgac ccaacgccgg cgcgagggtc
     1801 tgcgccagta caatcccggc cagcgcgtcg aggccttcgg gctcgaggcg gcgcgcgact
     1861 ggctgcgcga cgtcctgccc cggctggagc aatggactcc gctggaggag gtcgcgccga
     1921 cccgcagcag ccatgccgag ggcccgacgc aggccagctt taccgcctcg accctgtcgg
     1981 ccagtctgga gctggtcaag gagggggcga tgaacgtgag acaggccgag gccttcgccg
     2041 acctgtatct gaagcgccgg ggcaggggtc agccgctgga gctggttcct tgagtcggtt
     2101 cgccgccctt tcctcatcgt catcctcggg cttgacccga g
//
LOCUS       DASVVN010000035         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_421891,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000035 DASVVN010000000
VERSION     DASVVN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421891"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF686_00615"
     CDS             <1..422
                     /locus_tag="VF686_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HEX8660337.1"
                     /translation="NDQGAVIAAVGGQDDEGTELYVAASKLFLAQSVLVSGTVRFTNA
                     NQTGLLGFGGDANDDREAQFEGSVGWLLSRNLVVGAEYRTKPDNLSFAAEDDWFDVYA
                     AYAFNKHLSLTAAWADLGSIATFDDQRGLYISLQAGF"
     gene            424..942
                     /locus_tag="VF686_00620"
     CDS             424..942
                     /locus_tag="VF686_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX8660338.1"
                     /translation="MISRSMFTAVVALSFLAAGAAAAQDPAHRPGEEAVEAYAVADAN
                     ADAAPFTGTAMFEAFHGQAGIDRIVDAMLAATKADPRTAAIFRATDMVRLSRTLKEQF
                     CYLLGGGCGYTGRSMVDAHDDLGLQPADMGALVEHLQTAMAAEGVPFHTQNRFLAKLA
                     PMRRDIVTRQGG"
     gene            1041..>2685
                     /locus_tag="VF686_00625"
     CDS             1041..>2685
                     /locus_tag="VF686_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8660339.1"
                     /translation="MTSRADALRALLFASSAAGMLVAGPVLAEPGPDGEPQEATTLET
                     ITVDGRPVKREPTSPEFVAPLVDTPRSITIIPQQIIEQTAATSLQDILRTSPGITFGA
                     GEGGQPLADRPFIRGQASGNNVFVDGIRDSGGQQREVFNLEQVEVIKGPDSVYSGRGS
                     GGGSINLGSKAPRTTDFTRFALGAGTDGYLRATVDQNWHLGETAGLRVNLMGTEGDLP
                     GRDTVDFDKWGLGLSLAAGLGTPTTATFSYYHLTSNQMPDYGIPLFTKIGVRTTDSGI
                     LDVPYNSFYGLRDRDYLTNTVDTFTFDFEHAFSENLILRNVSRYSETLNDYIVTNPGD
                     GGGAQLVGGEWWMKRGTKSRWNPVTTIANVTDLRGSFVAGGMKHSFDLGLELSREDNS
                     NASYSTFTTAGAACPAGYNMSAANLNNLGAGDCTRVYAPSTGDAWAGVINRGPISNST
                     SESVGAYALDSMSLTERLILNLGVRWDRYAVSGMNGVPATIGGFTQVGGVWNPATYTA
                     VPEREWDFVNYQIGLVFKPTEDSSVYASYATSSTPPTIAA"
BASE COUNT          458 a          932 c          868 g          427 t
ORIGIN      
        1 tcaacgatca gggcgcggtc atcgcggcgg tcggcgggca ggatgacgag gggacggagc
       61 tctatgtcgc ggcgtcgaaa ctgtttctcg cgcagagcgt gctggtctcg gggactgtgc
      121 ggttcaccaa cgccaaccag accgggctgc tgggcttcgg cggcgacgcc aacgacgacc
      181 gcgaggccca gttcgaggga tcggtgggct ggctgctgag ccgcaatctg gtggtcggcg
      241 cagaataccg gaccaagccg gataatctgt ccttcgccgc cgaggacgac tggttcgatg
      301 tctatgcggc gtatgcgttc aacaagcacc tgtccctgac ggcggcatgg gccgacctgg
      361 gctccatcgc cacgttcgac gaccagcgcg ggctctatat ttctctgcag gcggggttct
      421 gaaatgatca gccgatccat gttcacggcg gtcgtggccc tttccttcct cgccgcgggc
      481 gcggccgcgg cgcaggaccc ggcccatcgg cctggcgagg aagcggtcga ggcctatgcc
      541 gtcgccgatg ccaatgctga cgcggcgccg ttcaccggga cggccatgtt cgaggccttc
      601 cacggacagg ccggcatcga ccggatcgtc gacgccatgc tggccgccac aaaggccgac
      661 ccccgaacgg ccgccatctt tcgggccacc gacatggtgc gcctgagccg cacgctgaag
      721 gaacagttct gctacctgct gggcggcggc tgcggctata cgggccggtc catggtcgac
      781 gcccacgacg atctgggcct gcagccggcg gacatgggcg cccttgtcga gcatctgcag
      841 accgccatgg ccgcggaggg ggtgcccttc cacacccaga accgttttct ggccaagctg
      901 gcgccgatgc gccgggacat cgtgacgcgt cagggcggat aacggcgtca tactgcgact
      961 tattcgcaat tggcgttgcg ctgaccggca aagctgatat tgcgacctga tcgcaactta
     1021 gcctcccccg gaccgatccc atgacctccc gcgccgacgc gcttcgcgcc cttctgttcg
     1081 cctccagcgc cgccggcatg ctggtggccg gtccggttct ggccgagccg ggcccggatg
     1141 gggaaccgca ggaagcgacg acgctggaga cgatcaccgt tgacggccgt ccggtgaagc
     1201 gcgagccgac cagccccgaa ttcgtggccc cgctggtcga caccccgcgc tcaatcacca
     1261 tcatcccgca gcagatcatc gagcagacgg cggcgacctc gctgcaggac atcctgcgca
     1321 cctcgcccgg catcaccttc ggcgcgggcg aaggcggcca gccgctggcc gaccggccgt
     1381 tcatccgcgg tcaggcctcg ggcaacaacg tcttcgtcga cggcatccgg gactcgggcg
     1441 gacagcagcg cgaggtgttc aacctcgagc aggtcgaggt catcaagggc cctgacagcg
     1501 tctattcggg acgcggctcg ggcggcggca gcatcaatct cggctccaag gcgccgcgga
     1561 ccacggactt cacccgcttc gctctgggcg cggggacgga cggctatctg cgggcgaccg
     1621 tggaccagaa ctggcatctg ggcgagacgg ccggcctgcg ggtcaatctg atggggacgg
     1681 aaggcgactt gcccggccgc gacaccgtcg acttcgacaa atggggcctg ggcctctcgc
     1741 tggcggcggg cctcggcacg ccgacgacgg cgaccttcag ctactatcac ctgacgtcca
     1801 accagatgcc ggactacggc atcccgctgt tcaccaagat cggcgtgcgc acgaccgaca
     1861 gcggcattct ggacgtgccg tacaacagct tctacggcct gcgcgaccgg gactatctga
     1921 ccaatacggt cgacaccttc accttcgatt tcgagcacgc gttcagcgaa aatctgatcc
     1981 tgcgcaacgt cagccgttat tccgagacgc tgaacgacta catcgtcacc aaccccggcg
     2041 acggcggcgg ggcccagctg gtcggcggcg aatggtggat gaagcgcggc accaaatcgc
     2101 gctggaaccc ggtcaccacg atcgccaatg tcaccgacct gcgcggttcc ttcgtcgccg
     2161 gcggcatgaa gcattccttc gaccttggcc tggagctgag ccgcgaggac aacagcaacg
     2221 ccagctattc caccttcacc accgccgggg ccgcctgtcc ggccggctac aacatgtcgg
     2281 cggccaatct gaacaacctc ggcgccggag actgcacccg cgtctacgcg ccctccacgg
     2341 gcgacgcctg ggccggggtg atcaaccgcg ggccgatcag caacagcacg tcggagagcg
     2401 tcggcgccta tgcgctggac agcatgtcgc tgaccgaacg actgatcctc aacctcggcg
     2461 ttcgctggga ccggtatgcg gtctcgggca tgaacggcgt cccggccact atcggcggct
     2521 tcacccaggt cggcggcgtc tggaatccgg ccacctatac ggccgtgccc gagcgggaat
     2581 gggacttcgt caactaccag atcggcctgg tgttcaagcc gaccgaggac tccagcgtct
     2641 acgcctcata cgccacctcc tcgaccccgc cgaccatcgc ggcgg
//
LOCUS       DASVVN010000036         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_53792, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000036 DASVVN010000000
VERSION     DASVVN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53792"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2353
                     /gene="putA"
                     /locus_tag="VF686_00630"
     CDS             <1..>2353
                     /gene="putA"
                     /locus_tag="VF686_00630"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX8660340.1"
                     /translation="RQAVAAAVRIMGEQFVLGRTIEGALKRADREGWLCSFDMLGEGA
                     RTAHDAERYEKIYADAIEAVGKTAKGQGPEAGHGVSVKLSALSPRYEATHEARVWDEL
                     YPRILRLARIAAKHDINFTMDAEEADRLALSLKLLDRLAREPDLGGWTGLGLAVQAYQ
                     KRCPEVIRRVAELAKTSGRRLMVRLVKGAYWDTEIKRAQQFGRTDYPVFTTKAATDLN
                     YLVCAKAMIEAAPHIYSQFATHNAHSLAAVHKMASERKVKIEFQRLHGMGEALYDAAR
                     DAFGPVTVRAYAPVGGHEDLLPYLVRRLLGNGANSSFVHALLDERVPASAVAADPISA
                     VELAPDRHAKIPTPKDMYMDRQNSLGRDYSQAADRDRHLAALEKVDAEKLTSGPIIGG
                     KLKAGTNPQPVTNPFDTTQVLGHVSEASTADIDAAIDAAARAQVAWDRKGGAGRAPVL
                     RAMADALEADMDRLVALLSREAGKTLNDAVAEIREAADFCRYYAMLAERDFGGRIELK
                     GPVGEINQLVLHGRGVFACISPWNFPLAIFTGQIVAALAAGNAVLAKPAEQTPLIAAE
                     AVRLYYKAGLDPNLLALVPGRGETVGAALVSHPGIDGVAFTGGTDTATMINKALAARP
                     GAIIPFIAETGGLNGMFVDTTALKEQVIDDVILSAFGSSGQRCSALRLLYVPHDAADA
                     LIEGLKGALAAQVVGDPADPSTDIGPVIDADAKAMLEAHLERLGGDAKIVARAELPAG
                     SDKGHLFAPTIAEIPTPDYLEREVFGPILHVCRYEPSKLKETAS"
BASE COUNT          380 a          918 c          736 g          319 t
ORIGIN      
        1 ccgccaggcc gtcgccgccg ccgtccgcat catgggcgag cagttcgtcc tgggccgcac
       61 catcgaaggg gcgctgaagc gggctgaccg cgagggctgg ctgtgcagct tcgacatgct
      121 gggcgagggc gcccgcaccg cccatgacgc cgagcggtac gagaagatct acgccgacgc
      181 catcgaagcc gtcggcaaga ccgcgaaagg gcagggcccg gaggccggcc acggcgtctc
      241 ggtcaagctg tcggccctgt ctccgcgcta tgaagcgacg cacgaggccc gcgtctggga
      301 cgagctctat ccccgcatcc tgcgcctcgc ccgcatcgcc gcgaagcacg acatcaactt
      361 caccatggac gcggaagagg cggaccgcct cgccctgtcg ctgaagctgc tggaccggtt
      421 ggcgcgcgag cccgatctcg gcggctggac cggcctcggc ctcgccgttc aggcctatca
      481 gaagcgctgc cccgaggtga tccgccgcgt cgccgaactg gcgaagacct cgggccgccg
      541 gctcatggtc cgtctggtca agggcgccta ctgggacacc gagatcaagc gcgcccagca
      601 gttcggccgc accgactatc cggtcttcac caccaaggcc gcgacagacc tcaactatct
      661 ggtctgcgcg aaggcgatga tcgaggcggc accgcacatc tacagccagt tcgccaccca
      721 caacgcccac tcgctcgccg ccgtccacaa gatggcttcc gagcgcaagg tgaagatcga
      781 attccagcgc ctgcacggca tgggcgaggc gctctatgac gccgcccgcg acgcctttgg
      841 acccgtcacc gtccgggcct acgcccccgt cggcgggcat gaggacctgc tgccctatct
      901 ggtgcgccgc ctgctgggga acggggccaa ctcctccttc gtccacgccc tgctggacga
      961 gcgggtgccc gcctccgccg tcgccgccga ccccatctcc gccgtcgagc ttgcgcccga
     1021 ccgccacgcc aagattccga ccccgaagga catgtacatg gaccgccaga actcgctcgg
     1081 ccgcgactat tcgcaagccg ccgaccgcga ccggcacctc gccgccctcg aaaaggtcga
     1141 cgccgagaag ctgacctcgg gcccgatcat cggcggcaag ctgaaggcag ggaccaaccc
     1201 ccagcccgtg accaacccct tcgacacgac gcaggtcctc ggccacgtct ctgaagccag
     1261 caccgccgac atcgacgccg ccatcgacgc cgccgcccgt gcgcaggtcg catgggaccg
     1321 caagggcggc gcgggccgcg cccccgtcct gcgcgccatg gccgacgcgc tggaagccga
     1381 catggaccgt ctggtcgccc tgctgtcgcg cgaggccggc aagacgctga acgacgccgt
     1441 cgccgagatc cgcgaggccg ccgacttctg ccgctactac gccatgctgg ccgaacgcga
     1501 cttcggcggc cgcatcgagc tgaagggccc ggtcggcgag atcaaccagc tggtcctgca
     1561 tggccgcggc gtcttcgcct gcatcagtcc gtggaacttc cctctggcca tcttcaccgg
     1621 ccagatcgtc gcggccctgg ccgccggcaa cgccgtgctg gccaagcccg ccgaacagac
     1681 gcccctgatc gccgcggagg ccgtgcgcct ctattacaag gccggcctgg accccaacct
     1741 gctggccctc gtccccggac gcggcgagac cgtaggcgcg gctctcgtca gccatcccgg
     1801 catcgacggc gtcgccttca ccggcggcac cgacacggcg accatgatca acaaggcgct
     1861 ggccgcccgc ccgggcgcca tcatcccctt catcgccgag accggcggcc tgaacggcat
     1921 gttcgtcgac accacggcgc tgaaggaaca ggtcatcgac gacgtcatcc tgtcggcctt
     1981 cggctcctcg ggccagcgct gctcggccct gcgcctgctc tacgtcccgc atgacgccgc
     2041 cgacgccctg atcgagggcc tgaagggcgc gctggcggcg caggtcgtgg gcgatcccgc
     2101 cgacccctcc accgacatcg gccccgtcat cgacgccgat gcgaaggcca tgctcgaggc
     2161 ccacctcgaa cgcctcggcg gagacgcgaa gatcgtcgcc cgcgccgaac tgcccgccgg
     2221 ttcggacaag ggccatctgt tcgcgccgac catcgccgag atcccgaccc ccgactatct
     2281 ggagcgcgag gtcttcggcc ccatcctgca cgtctgccgc tacgagccgt cgaagctcaa
     2341 ggagacggcc tcg
//
LOCUS       DASVVN010000037         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_343248,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000037 DASVVN010000000
VERSION     DASVVN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343248"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VF686_00635"
     CDS             complement(<1..738)
                     /locus_tag="VF686_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8660341.1"
                     /translation="MTERLKGWNRFCLAALLGGALAGAASPVWAQPTTTGLEALRQRP
                     AEDEVIYFLMPDRFENGDTANDRGGAEGDRLLTGYDPTDTGFYHGGDLKGLTARLDYI
                     RGMGATALWIAPPFRNKWVQGPPGHESAAYHGYWITDFTQIDPHLGTNEEFRALVDAA
                     HARGMKVYLDIVINHTADVIRYRECPQNDCGYRWKGDYPYQRRGGLQGEPINPGFTGA
                     PGGDFSTLVSPDYAYTPYVPAGEETTKS"
     gene            1065..>2748
                     /locus_tag="VF686_00640"
     CDS             1065..>2748
                     /locus_tag="VF686_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8660342.1"
                     /translation="MLAGGAVGAIGLVAFAGAASAQSTQEPTEVDEVVVTGIRSSIES
                     SIASKRTETSIVEVVTAEDIGKLPDVSIAESLARLPGLTAQRLDGRGQVISIRGLAPD
                     FTTALLNGREQVSAGDNRGVEFDQYPSELLGSVMVYKTPDAALIGQGLAGTADLRTIR
                     PLEHGRQTIALNARYEWNDYGALNSGTEDTGQRLSVSYIDQFMDGKLGLVLGYAHMST
                     PYQAERFNAWGYPDSGPGGALLIGGAKPYVQSSELVRDGYMATVEFRPNDRWHSTFDA
                     FYSEFDNTQILRGVEFPLAWGGSAGACPGPAATCRPAPALRAGATVEDGMVVAGTFDN
                     VKGVVRNDGNRRTAELSSFAWENEFMIDDNWSATLDLSRSKADRTDTVLETNAGTGRN
                     ITGALDTLTFSTTPEGTTFSSLLNYADPTLILLTSAQGWGGGVYPAGIEGGQDGFLNS
                     PSIVDELNSIRGEVAGDIEAGPFSRITFGINVTDREKELTLERYYLSLTANRNDPLHN
                     TSVPVPSQFLLAPTQLDFLGIGPVISYDPFALVESGIYTLLAPQDDNLLAFGW"
BASE COUNT          454 a          939 c          897 g          458 t
ORIGIN      
        1 ggatttggtg gtctcctctc cggccgggac gtacggcgta taggcatagt cggggctgac
       61 gagagtcgag aagtcgccgc cgggggcgcc ggtgaagccg gggttgatcg gctcgccctg
      121 caggccgccg cgccgctgat acggatagtc acccttccag cggtagccgc agtcgttctg
      181 cgggcactcg cggtagcgga tgacgtcggc ggtgtggttg atgacgatgt ccagatagac
      241 cttcatcccg cgcgcatggg cggcgtcgac gagggcgcgg aactcctcat tggtcccgag
      301 atgcggatcg atctgcgtga agtcggtgat ccagtagccg tgataggccg cgctctcgtg
      361 accgggtgga ccctgcaccc atttgttccg gaacggcggc gcgatccaca gggcggtggc
      421 gcccatgccc cggatatagt cgaggcgcgc ggtcaggcct ttcaggtcgc cgccgtgata
      481 gaagcccgtg tcggtcgggt cgtaacctgt gagcaggcgg tcgccctcgg cgccgccccg
      541 gtcgttggcc gtgtcgccgt tctcgaaccg gtcgggcatc aggaaataga tgacctcgtc
      601 ctcggccgga cgctggcgca gcgcctccag accggtcgtc gtcggctggg cccagacagg
      661 cgatgcggcg cccgcgagcg cgcctcccag cagcgcggcg agacaaaacc gattccaccc
      721 cttgaggcgt tccgtcatgg cctggcgtcc tctccatgtc cgcgaacgac gtggctcgct
      781 ggatcagttt gcaagatttt gcagtgccgg ggccgtctgt caatccaccg cgtcgtttgt
      841 ccgaaatgcg gcggtgccgt gctgaatcgc ctggtgcagt gcagtatagc cttgcgacgc
      901 gtcacatttc cgccacgccg ccatcgctgc cgtatcatta ccggcttgca aggttcccat
      961 aagtttgcat aactttgcgg tgacaggtcg gacgcttcga cagaaatgcg ccggctccag
     1021 ggagaggaat gacgatgaga actccaatca cgcgccgtgc ccgcttgctc gcaggcggcg
     1081 ctgtcggcgc catcggcctc gtggctttcg ccggcgcggc ctcggcccag agcacgcagg
     1141 agccgaccga ggtcgacgag gtcgtcgtca ccggcatccg ctcctcgatc gaaagctcga
     1201 tcgcgtccaa gcgcaccgaa acctcgatcg tcgaggtcgt gaccgccgag gacatcggca
     1261 aactgcccga cgtctcgatc gccgagtcgc tggcccgact gccgggtctg acggcacagc
     1321 ggcttgatgg acgcggtcag gtgatctcga tccgcggtct cgcgcccgac ttcaccaccg
     1381 cgctgctgaa cggccgcgaa caggtctcgg ccggcgacaa ccgcggcgtc gagttcgacc
     1441 agtatccgtc cgaactgctg ggcagcgtca tggtctacaa gaccccggac gccgctttga
     1501 tcgggcaggg tctggccggc accgccgacc tgcgcacgat ccgcccgctg gagcacggcc
     1561 gccagacgat cgcgctgaac gcccgctatg agtggaacga ctacggcgcg ctgaactcgg
     1621 gcaccgagga cacgggccag cggctcagcg tcagctacat cgaccaattc atggacggca
     1681 agctgggtct ggtgctgggc tatgcccata tgtcgacgcc ctatcaggcc gagcgtttca
     1741 acgcctgggg ctatccggac tcgggtccgg gcggcgcgct gctgatcggc ggcgccaagc
     1801 cctatgtcca gtccagcgaa ctggtgcgcg acggctatat ggcgaccgtc gagttccgcc
     1861 cgaacgaccg ctggcactcg accttcgacg ccttctattc cgagttcgac aacacccaga
     1921 tcctgcgcgg cgtcgaattc cccctcgcct ggggcggcag cgccggggcc tgccccggac
     1981 cggcggcgac ctgccgcccg gctcccgccc tgcgcgccgg cgccactgtc gaggacggaa
     2041 tggtcgtggc cggcaccttc gacaatgtga agggcgtcgt ccgcaacgat ggcaaccgcc
     2101 gcaccgccga gctgagctcg ttcgcgtggg aaaacgagtt catgatcgac gacaactggt
     2161 cggcgaccct ggacctcagc cgttcgaagg ccgaccgcac cgacacggtg ctggagacca
     2221 acgccggcac cggccgcaac atcaccggcg ccctggacac cttgaccttc agcacgacgc
     2281 cggagggcac gaccttcagc tcgctgctca actatgctga tccgaccctg atcctgctga
     2341 ccagcgccca aggctggggc ggcggcgtct atccggccgg catcgagggc ggccaggatg
     2401 gtttcctcaa cagcccgtcg atcgtcgacg aactgaactc gatccgcgga gaagtggcgg
     2461 gcgacatcga ggccgggccg ttcagccgga tcacgttcgg catcaacgtc acggaccgcg
     2521 agaaggaact gacgctcgag cgctactacc tgtcgctgac ggccaaccgc aacgacccgc
     2581 tgcacaacac cagcgtgccc gtgcccagcc agttcctgct cgcgccgacg cagttggact
     2641 tcctcgggat cggccccgtg atcagctacg acccgttcgc cttggtcgag agcggcatct
     2701 acaccctgct ggcgccgcag gacgacaacc tgctggcctt cggctggg
//
LOCUS       DASVVN010000038         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_1431, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000038 DASVVN010000000
VERSION     DASVVN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF686_00645"
     CDS             <1..429
                     /locus_tag="VF686_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8660343.1"
                     /translation="YLEHIADHSTAYPGSLEALEQLAARGAILCVATNKPTDLAVALF
                     DAIGLTDRFAVICGPQSVSAKKPDAAHIRETVLLAGGDPARAIMVGDSITDLDAARAT
                     GVPCILTTFGYTDIPAAQLGADAVIAHFDELPAAIDRMVG"
     gene            502..>1595
                     /locus_tag="VF686_00650"
     CDS             502..>1595
                     /locus_tag="VF686_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8660344.1"
                     /translation="MLRRFFAIPLWQRTAAGFALGILAGLILREQAEVWLQPIGDVYL
                     NLIRMVVAPLVLFTIASSIAKLGEGAGAVRLGLRTLVWFAITSLLAVLVGFAFGHLIN
                     PGLGLENLPLGEVKERVIPTPLEVLIGIVPTNPFAALSEGKVLQIIFFSALVGGALVA
                     LGDRAATARRFVDEGSAIVFRITRWVIQLTPFGVFGLIGAVVGGYGWEALLPLGKFIL
                     AIYAACLFHILVVYSGLLKLHGLKVVNFFRGAFAAQQTAFATSSSLGTLPITLRQTVE
                     RLGVPQAYAAFAVPLGANVKMDGCGAIYPAIAAIFIAQYFSIDLSLTQYVLIGLTAVL
                     GSLGTAGVPGTSIVMLTLTLTTAGLPLEGIG"
BASE COUNT          213 a          570 c          511 g          301 t
ORIGIN      
        1 tatctcgagc atatcgcgga ccacagcacg gcctatcccg gttcgctcga ggcgctggag
       61 cagctggcgg cgcgcggcgc catcctgtgc gtcgccacca acaagcccac cgatctggcc
      121 gtggccctgt tcgacgcgat cggcctgacc gaccgtttcg ccgtcatctg cgggccgcaa
      181 tccgtgtctg cgaagaagcc ggacgccgcc catatccgcg agacggtgct tctggccggc
      241 ggtgatccgg cgcgggcgat catggtgggc gacagcatca ccgatctgga cgccgcccgc
      301 gcgaccggcg tgccgtgcat actgaccacc ttcggctaca ccgacatccc cgccgcccag
      361 ctgggcgcgg acgccgtcat cgcccatttc gacgagcttc ccgcggctat cgaccgaatg
      421 gtcggctgaa tccgcagccc cggcggttgc ggatcgtcgg gagagccaac atctgcacgg
      481 ttcgtcagat cgaggccgtc catgctccgc cgcttcttcg cgattcctct gtggcagcgc
      541 accgccgccg gtttcgcgct cggcatcctc gccggtctga tcctgcggga acaggccgag
      601 gtctggctgc agcccatcgg cgacgtctat ctgaacctga tccgcatggt ggtcgcgccg
      661 ctggtgctgt tcaccatcgc cagctcgatc gcaaaactgg gcgaaggcgc cggcgcggtg
      721 cggctggggc tgcggaccct cgtctggttc gccatcacct ccctgctggc ggtgctggtc
      781 ggtttcgcct tcggtcacct gatcaacccg ggcctcggtc tggagaacct tccgctgggc
      841 gaggtgaagg aacgcgtcat cccgaccccg ctggaggttc tgatcggcat cgtccccacc
      901 aatcccttcg cagcgctcag cgagggcaag gttttgcaga tcatcttctt ctccgcgctc
      961 gtaggcggcg ccttggtggc gctcggcgac cgggcggcga cggcgcggcg tttcgtcgac
     1021 gagggctccg ccatcgtctt ccggataacc cgctgggtca tccagctgac cccgttcggg
     1081 gtgttcggcc tgatcggcgc ggtcgtcggc ggctacggct gggaggcgct gctgccgctc
     1141 ggaaagttca tcctcgccat ctacgccgcc tgcctgttcc acatcctcgt ggtctattca
     1201 ggcctgctga agctgcacgg cctgaaggtc gtcaacttct tccgcggcgc ctttgcggcc
     1261 cagcagacgg ccttcgctac ctcgtcgtcg ctcggcaccc tgccgatcac cctgcgccag
     1321 acggtcgagc ggctgggtgt gccgcaggcc tatgccgcct tcgcggtgcc gctgggggcc
     1381 aacgtcaaga tggacggctg cggcgccatc tacccggcca tcgccgccat cttcatcgcc
     1441 cagtatttca gcatcgacct gtcgctgacc cagtatgtgc tgatcggcct gacggcggtg
     1501 ctgggctcgc tgggcaccgc gggcgtgccg gggacgagca tcgtcatgct gaccctgacc
     1561 ctgaccaccg ccggtctgcc gctggaaggc atcgg
//
LOCUS       DASVVN010000039         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_107036,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000039 DASVVN010000000
VERSION     DASVVN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107036"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF686_00655"
     CDS             <1..187
                     /locus_tag="VF686_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660345.1"
                     /translation="PLSGGLADEIRLSVDDPALKELAQNGKMAVEGDAGAFELTATRA
                     ERKLIDEFFAHCGHRGV"
     gene            223..648
                     /locus_tag="VF686_00660"
     CDS             223..648
                     /locus_tag="VF686_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660346.1"
                     /translation="MFAFLAAAILAVQTPAPAAGWTWTLYADDTPVVLANEIPDTANL
                     RATLECDPGTSVARLTLYGTPLTGMARVAAGEAVAMAEAAPARGDATRLMLRTDHPAF
                     AAFAADGKMNVLVGDHTRPVEVPAGHLAKLRRFSELCSG"
     gene            662..1162
                     /locus_tag="VF686_00665"
     CDS             662..1162
                     /locus_tag="VF686_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660347.1"
                     /translation="MRTTILALAALAVTASACATPGPDVPTPPASSGSPAAVEGYDWF
                     FHADEDAARLAYGLAESDDLRLGLDCERASGRLSLSGMTQDATKPEFYIESGGETGRF
                     PASAEPSQVSDGVFLSAEAPADAPVFRRFRQVGWLALWQDGTRQAYAPHPASAPNIER
                     FFAFCG"
     gene            1211..>2186
                     /locus_tag="VF686_00670"
     CDS             1211..>2186
                     /locus_tag="VF686_00670"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8660348.1"
                     /translation="MKTVIAASAIALLAGFAQPAVAQTAAPVAVAPAAPNAALASVLA
                     DYETWLRAVEPVTAGMEGDRAALSRLGDASRAFEVAQEPVLKGFADRLAAIDPAGLTD
                     DERLNHAFMTYVVKRSRDRIPLDTGRIDAFNSEGGPGQGLSYIASITRITTAADAEAW
                     IARLEAMPKVYADQLANARRGLETGMVQPRSIVENALTLIEPEAALTPGADPLLKPFA
                     ALPASIPVAQQNALRERAARAVADGVMPQRREWARFLREDYLPRAPETLGLVNRPGGR
                     EQYAYLVRGFTTTELTPDEVHAIGLEEVARIRARMDTEMRAAGWTGDFA"
BASE COUNT          324 a          812 c          733 g          317 t
ORIGIN      
        1 tcccctgagc ggcggactgg cggatgaaat ccggctgtcg gtcgatgatc cggccctgaa
       61 ggaactggcg caaaacggca agatggctgt cgagggcgac gccggcgcgt tcgagctgac
      121 cgccacccgg gccgagcgca aactgatcga cgagtttttc gcccattgcg gacatcgcgg
      181 cgtttgacca cagtcggtcg aaccttcagc gggggctgaa ccatgtttgc ctttctcgcc
      241 gccgccatcc tggccgtgca gacgccggcg cccgccgccg gctggacatg gacgctgtac
      301 gccgacgaca cgccggtcgt gctggccaat gaaatcccgg acaccgcaaa cctgcgagcc
      361 acgctggagt gcgatccggg gaccagcgtc gcccggctca ccctgtatgg aacgcccctg
      421 acgggcatgg cccgggtcgc cgcgggtgag gctgtcgcca tggccgaggc cgcgcccgcg
      481 cgcggcgacg ccacccgtct gatgctgagg acggaccacc ccgctttcgc cgccttcgcg
      541 gccgacggaa agatgaatgt tctggtcggc gatcacacgc gaccggtcga agttcccgcg
      601 ggccaccttg ccaagctgcg ccgtttctcg gaactgtgtt cgggctgaac ccggagccga
      661 catgcgcacc acgatcctcg ctctggccgc tctcgcggtg accgcctcgg cctgcgcgac
      721 ccccgggcct gacgtcccga cgccgccggc ctccagcgga tcgccggctg cggtcgaggg
      781 ctacgactgg ttcttccacg ccgatgagga cgccgcccgg ctcgcctacg gtctggccga
      841 gagcgacgat ctgcggctgg ggctggactg cgagcgcgca tccggtcggc tgtcgctcag
      901 cggcatgacc caggacgcaa ccaagccgga gttctacatc gaatccggcg gcgagaccgg
      961 acgcttcccc gcctcggcgg aaccgtcaca ggtcagcgat ggcgtcttcc tgagcgccga
     1021 ggcgcccgcc gacgctccgg tgttccggcg gttccgccag gtgggctggc tggccctgtg
     1081 gcaggacggg acgcgtcagg cctatgcgcc ccatccggcg tcggcgccca atatcgagcg
     1141 gttcttcgcc ttctgcggtt gacgcgggac gccggctccg gtgcagtccc tcatcctcgg
     1201 gagggatttc atgaaaaccg tcatcgccgc cagcgccatc gcactcctcg ccggcttcgc
     1261 ccagccggcc gtcgcccaga cagccgcgcc cgtcgcggtc gcccccgcgg ccccaaatgc
     1321 agcgctggcc tcggttctgg cggactatga gacctggctg cgcgcggtgg agccagtcac
     1381 ggcgggcatg gaaggcgacc gcgcggccct gtcacggctg ggtgacgcct cgcgcgcttt
     1441 cgaggtggcg caggagccgg ttctcaaggg gttcgcggac aggctggcgg cgatcgatcc
     1501 cgccggcctc accgacgacg agcggctgaa ccacgccttc atgacctatg tcgtgaaacg
     1561 gtcccgcgat cgcatcccgc tcgacaccgg ccgcatcgac gccttcaact ccgagggcgg
     1621 accgggtcag ggcttgagct acatcgccag catcacccgg atcaccaccg ccgccgacgc
     1681 cgaggcctgg atcgcgcggc tggaggccat gcccaaggtc tatgccgacc agctggccaa
     1741 cgcccggcgc gggctcgaga ccgggatggt gcaaccccgc tccatcgtcg agaacgccct
     1801 gaccctgatc gagcccgagg ccgccctgac acccggggca gaccccctgc tgaagccgtt
     1861 cgccgccctg cccgcctcca tcccagtcgc ccagcagaat gcgttgcgcg agcgcgcggc
     1921 ccgcgccgtg gccgatggcg tcatgccgca acgccgggaa tgggcgcggt tcctgcgcga
     1981 ggactacctg ccccgcgcgc ccgaaaccct ggggctggtc aaccgaccgg gcgggcgcga
     2041 gcaatacgcc tatctggtgc gcggcttcac caccaccgag ctgacgccgg acgaggtcca
     2101 cgccatcggc ctcgaggagg tcgcccgcat ccgcgcccgc atggacaccg agatgcgcgc
     2161 cgccggctgg acgggcgact tcgccg
//
LOCUS       DASVVN010000040         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_343261,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000040 DASVVN010000000
VERSION     DASVVN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343261"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1569
                     /locus_tag="VF686_00675"
     CDS             34..1569
                     /locus_tag="VF686_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8660349.1"
                     /translation="MNWTILLLGFAGMVIGQFMAILDIQIVASSLPQIQAGVGASADE
                     ISWIQTAYLIPEVVMIPLSGYLSRLWGTQKVFLVSCAGFLLMSVATGLSTSIEAMIFF
                     RALQGFVGGAMIPTVFAVAFTAFPPDKRVTASIVMGLIVTLAPTVGPTLGGHLTEWLS
                     WRWLFFINVIPGMLVLFLVGRFANFDRGDPSLAKGFDWAGLGLMATFLMSLQFVLEEG
                     SKNDWFADDMILLLAVVAAITGPAFVWRSLSYRNPIVELRAFANRNFLVGVVMTFTVG
                     AALFGGTFLLPLFLGRVRNYSASEVGTTMVISGLAMFLTAPFAGRLVRKVDLRILMFG
                     GFMLCAWGMWDARLVTGDWGFWEFAGVQAVRGCGVMLAMIAAQQVTMSTLPPHMVKNA
                     SGLVNLSRNVGGAFGLALLNTSLTTNTAAHMSELTQNISVTNGGMQAMLAGMQERFAG
                     SIDPAGAAMKAIYGMLYKQATTLAFGDAFAMLAVGCAFAAFVTLLSRPVKAPAGAPAA
                     EAH"
     gene            1569..2162
                     /locus_tag="VF686_00680"
     CDS             1569..2162
                     /locus_tag="VF686_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8660350.1"
                     /translation="MPRIAGQIDEAKTEAILEAASALFGEKGALASMEQIARRAGVSR
                     QTLYNRFPNKLEIGRALAARRSDAISAPLRSGGDPQTVLAALAASLLDKLCRTEAGAS
                     LRGVALMSPELPELADAIYQAGPAEGLRRLSAWLSEQNRLGLLAVPDPDAAAEMFSGM
                     VLGHGHLRGVLGVAHPAFDRDARAAEAARRFLRAFAV"
     gene            2223..>2506
                     /locus_tag="VF686_00685"
     CDS             2223..>2506
                     /locus_tag="VF686_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8660351.1"
                     /translation="MLIHLSSWPEIDARLKTTKTVVVPIGSNEQHGPTGLLGTDWLCP
                     EIIAYAAEKQDESLVVAPTFNIGMAQHHLAFAGTISLRPSTFMAAITDWV"
BASE COUNT          336 a          877 c          844 g          449 t
ORIGIN      
        1 ccaccggcgg ctgccgccac ggccgagccg cagatcaact ggacgatcct gctgctgggc
       61 ttcgccggca tggtgatcgg ccagttcatg gccatcctcg acattcagat cgtcgcctcg
      121 tccctgccgc agattcaggc cggggtcggc gcctcggccg acgagatcag ctggatccag
      181 accgcctatc tgatccccga ggtcgtgatg atccccctgt ccggctatct gtcgcggctg
      241 tgggggacgc agaaggtgtt tctggtctcc tgcgccggct tcctgctgat gagcgtggcg
      301 accggcctgt cgacctcgat tgaggcgatg atcttcttcc gcgcgctgca gggcttcgtc
      361 ggcggggcca tgatcccgac cgtcttcgcc gtcgccttca ccgccttccc gccggacaaa
      421 cgggtcacgg ccagcatcgt catgggcctg atcgtgacgc tggcgccgac cgtcggaccg
      481 accctcggcg gacatctgac ggaatggctg agctggcgct ggctgttctt catcaacgtc
      541 attccgggca tgctcgttct gttcctggtc ggccgcttcg ccaatttcga ccggggcgat
      601 ccgtcgctgg ccaagggctt cgactgggcc ggactgggcc tgatggccac cttcctgatg
      661 agcctgcagt tcgtgctcga ggaggggtcg aagaacgact ggttcgccga cgacatgatc
      721 ctgctgctgg ccgtggtcgc cgccatcacc gggcccgcct tcgtctggcg ctcgctgagt
      781 tatcgcaatc cgatcgtgga gttgcgcgcc ttcgccaacc gcaacttcct cgtcggggtg
      841 gtgatgacct tcaccgtcgg ggcggccctg ttcggcggca cgttcctgct gccgctgttc
      901 ctcggccggg tgaggaacta ttcggcgtcc gaggtgggga ccaccatggt catttcgggt
      961 ctcgccatgt tcctgaccgc ccctttcgcc gggcggctgg tgcgaaaggt cgatctgcgc
     1021 atcctgatgt tcggcggctt catgctgtgc gcttggggca tgtgggacgc gcggctggtg
     1081 acgggcgact ggggcttctg ggagttcgcc ggcgtgcagg ccgtgcgcgg ctgcggcgtc
     1141 atgctggcca tgatcgcggc ccagcaggtg accatgtcaa ccctgccgcc gcacatggtc
     1201 aagaacgcct ccgggctggt gaacctgtcc cgcaacgtcg gcggcgcctt cggtctggcc
     1261 ctgctgaaca cctcgctgac caccaacacc gccgcgcaca tgagcgagct gacccagaac
     1321 atctccgtga ccaacggcgg catgcaggcc atgctggccg ggatgcagga gcggttcgcc
     1381 gggtccatcg atccggcggg cgcggcgatg aaggcgatct acggcatgct gtacaaacag
     1441 gcgacgacgc tggcgttcgg cgacgccttc gccatgctgg cggtgggctg cgccttcgcc
     1501 gccttcgtca ccctgctgtc gcgcccggtg aaggcgccgg ccggagcgcc ggccgcggag
     1561 gcgcactgat gccgcgcatc gccgggcaga tcgacgaggc caagaccgag gccatcctcg
     1621 aggcggcgtc ggcgctgttc ggggagaagg gcgccttggc ctcgatggag cagatcgccc
     1681 gccgcgccgg ggtgtcgcgc cagaccctct acaaccgttt tcccaacaag ctggagatcg
     1741 ggcgcgccct cgccgcccgc cggtccgacg ccatcagcgc gccgctgcgt tccggcggcg
     1801 acccgcagac ggtgctggcc gccctggccg ccagcctgct ggacaagctg tgccggaccg
     1861 aggccggggc gtcgctgcgc ggcgtggccc tgatgtcgcc ggagctgccg gaactggccg
     1921 acgccatcta tcaggccggc cccgccgaag gcctgcgccg cctttcggcg tggctgtcgg
     1981 aacagaatcg gctcggtctg ctggccgttc ccgaccccga cgccgcggcc gagatgttct
     2041 cgggcatggt gctgggccac ggccatctgc gcggcgtgct gggcgtggcg catccggcct
     2101 tcgaccgcga cgcccgcgcc gccgaggcgg cccgccgctt cctgcgcgcc ttcgccgtct
     2161 gagtggttgg cctgccgacc caatgggcct agaccgcgcc gataccgttg cggagcgttc
     2221 ccatgctgat ccacctctcc tcatggcccg agatcgatgc gcggctgaag acgacgaaga
     2281 ccgtcgtcgt gcccatcggc tccaatgaac agcacggccc gaccggcctg ctgggcaccg
     2341 actggctgtg ccccgagatc atcgcctatg ccgccgagaa gcaagacgag agtctggtgg
     2401 tggcgccgac cttcaacatc ggcatggccc agcatcatct ggccttcgcg ggcaccatct
     2461 ccctgcgccc gtcgaccttc atggcggcga tcaccgactg ggtcag
//
LOCUS       DASVVN010000041         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_396154,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000041 DASVVN010000000
VERSION     DASVVN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396154"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF686_00690"
     CDS             <1..497
                     /locus_tag="VF686_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX8660352.1"
                     /translation="VLTFLSPESDPSGTGYNIIQSKIALGSGGLLGKGYGLGSQSQLE
                     FLPERHTDFIFSTVAEEFGFVGSFTVLACYVAIILIALRIASLAHSHFGRIAAAGMTA
                     FFALFVLINGAMVMGMAPVVGVPMPLLSYGGSSMMTVMFGFGLVLATRVHRYAELPKG
                     HGLF"
     gene            526..1014
                     /locus_tag="VF686_00695"
     CDS             526..1014
                     /locus_tag="VF686_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6173 family protein"
                     /protein_id="HEX8660353.1"
                     /translation="MSKPDPAPSDPAPDAPVVPASLFQSDCGDKPCPEPVLPTATPEQ
                     QAALTDGVRMRENPAEWAFVRLSKLIEDFEKNLDRDEEVGARIVGLPGDGTMSIEDVG
                     FWGPDLIMFLGKNADGKPVRLIQHYAQINVLLDVRKKPEERAARRIGFQLSEMVEKTN
                     PK"
BASE COUNT          275 a          576 c          546 g          269 t
ORIGIN      
        1 gggtgctcac cttcctcagt cccgaatccg acccgtccgg cacgggctac aacatcatcc
       61 agtcgaagat cgccctcggc tctggcgggc tgctcggcaa gggctacggc ctcggcagcc
      121 agagccagct ggagttcctg cccgaacggc atacggactt catcttctcg acggtggccg
      181 aggagttcgg cttcgtcgga tcgttcacgg tgctggcctg ctacgtcgcc atcatcctga
      241 tcgccctgcg catcgcctct ctggcgcaca gtcatttcgg ccgcatcgcc gccgccggga
      301 tgacggcctt cttcgccctg ttcgtgctga tcaacggcgc catggtcatg gggatggcgc
      361 cggtggtcgg cgtgcccatg ccgctgctgt cctacggcgg ctcgtcgatg atgaccgtca
      421 tgttcggctt cggcctggtg ctggcgacgc gcgtgcaccg ctacgccgaa ctgcccaagg
      481 gccacggact gttctgaatc ggctctcgtg cgttagggtc ggaccatgag caaacccgat
      541 cccgcaccct ccgaccccgc gcctgacgcg cccgtggtcc cggcctcgct gttccagtcg
      601 gactgcggcg acaaaccctg tccagagccg gtcctgccca cggccacgcc ggaacagcag
      661 gcggccctga ccgacggcgt gcggatgcgc gaaaaccccg ccgaatgggc ttttgtccgc
      721 ctgtccaagc tgatcgagga cttcgagaag aacctcgacc gggacgagga ggtcggcgcc
      781 cgcatcgtcg gcttgccggg cgacggcacc atgtcgatcg aggacgtcgg tttctggggg
      841 ccggacctga tcatgttcct gggcaagaac gccgacggca agccggtgcg cctgatccag
      901 cactacgccc agatcaacgt cctcctggac gtccgcaaga agcccgagga acgcgccgcg
      961 cgccgcatcg gcttccagct gtcggaaatg gtcgagaaga ccaatccgaa gtaggccgct
     1021 gtcgtcatcc cggggcgcgc agcgaccccg ggacccagcg gcgcgcgaga gcgcgaagct
     1081 gtcggggaca ctctgcctgg acagatcgcc ttcggcgccg ctgggtcccg gccttcgccg
     1141 ggatgacgac aaggaaagcg gatgacgctc cccgccaata tagtcgcatt ggcgaaaaaa
     1201 tatcctgcct ttccagatac atccgctttt gacagcggct cttcctcggc gccgctccca
     1261 gcgcccccat aatcctcctc atcccgtcgc gggggagggc gtctggcgcc agggccatca
     1321 acgtgcggcg gggtgcgatc gagcgggaaa cgtcgtcctg taatcgtcgc ccggaccctt
     1381 gcgcggctca tacctgcggc ttgtggaaca tcgggggcgt gggggcggcg gattgcccgg
     1441 gggctgtgaa accccggggt ccggagggcc tggaagctct ccgcccgtcg cgggcttacg
     1501 acggacaggg ctgaatgcgg cgacaaggcc ggggcgggaa aactccgcaa accccgcgag
     1561 ccgccaccgg aagcccgccc ccgtcggctt gcgtgaaccg gtcgcgcgga gcgaaggcgg
     1621 cgacgccgaa aacacatcaa cggtccccgt aacgcgaaag cagggg
//
LOCUS       DASVVN010000042         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_264439,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000042 DASVVN010000000
VERSION     DASVVN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264439"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VF686_00700"
     CDS             complement(<1..1563)
                     /locus_tag="VF686_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX8660354.1"
                     /translation="MNIVIIVGLIITFATGIPVLMQILKNHPRGLIILFFAEMWERFS
                     YYGMRGILIFFLTQHFLFDDAMAGSTYGSYTSLVYLLPLVGGILADRFIGTRKAIAFG
                     AVLLVAGHGLMAFEGRPATETLTYAGQTYEISAEGRGADRDVNIVVDGQKYAFGPAET
                     GGIAVTGLPAASPLPATLAEGSYVMTQTRDLMGVNVFYLAVSLIIMGVGFLKPNISTI
                     VGQLYPQGDPRRDSGFTLYYYGINLGAFWAAVLCGYLGQTVGWWAGFGLAGLGMALGW
                     VVFMLGKPLLEGKGEPPNPEMLKRPMIGPLNREWSIYLLATLGVGLVWFLVQRNALVG
                     TVLTAATIASLLFILYVIIKVCQTRAQRERMMLAVVLIFGSVVFFTLFEQAGTSLNLF
                     ADRNVDLSLTPTAFNLLGVTVGTPAQLAAAGITPTGFWIDATITAAQTQSFNAGFILL
                     FAPIMAALWAYLAKRNMDPNPTMKFGLGLLQVGLGFMVVVWGAGMANSAFQLPIVLLG
                     LLYMFHTTGELFL"
     gene            1799..2749
                     /locus_tag="VF686_00705"
     CDS             1799..2749
                     /locus_tag="VF686_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660355.1"
                     /translation="MTTTRRSALIGLGGGLTLSACSLGPSRDDSGNRDVVAAGSPAAL
                     LVWAVARERLAGWPRKPAQSALSALPAFAAGLPELGGLTGNNRHANLEAVAALKPRLI
                     IDYGDIDPEYQVLAQRLKVQLGADWRLIDGALSRIPEAIREAGALLGAAPKADPLADN
                     AAEVLERWRQAPPGPSFYYARGGDGLETGFRGALATEVLEGAAWTNVAEGSRDIGRVT
                     REQVAAWDPEVLVTLDRGFAESVRSDPVWRWRRNGLRRRLLLLPDRPFGWIDRPPSVN
                     RLLGCAWLADPQGPTLTLAALSRRLYGMAPVEIAQPRWIS"
     gene            2737..3735
                     /locus_tag="VF686_00710"
     CDS             2737..3735
                     /locus_tag="VF686_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX8660356.1"
                     /translation="MDFLNRLRSSLPALALTLLALACAAGVLSTGPLAIPPADLLSAL
                     IGQGGPDAELVLTLRGPRLLAALTCGAALAGAGAAFQILFRNPLAAPDLLGVSSGAGL
                     GAALALLLGAGTVLVQSAAFAGGLAIGGLALGCAALTRRGDGRLALILCGVVAGALAS
                     AGLGLVVIVAEPYSQLPAITYWLLGSFTRATLAEALFALAPICFGGAVLLWLGFRLDV
                     LSLGDEQARSLGLPARRLRLTALAAAALMTSAAVALAGMVGWIGLLAPHAARLIVGER
                     AGQLIPASMAIGALFALVIDRLATAFGPAEIPVGLLSAALGAPAFLFLFVAASRRP"
     gene            3732..4451
                     /locus_tag="VF686_00715"
     CDS             3732..4451
                     /locus_tag="VF686_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8660357.1"
                     /translation="MSSLLLEGLVAGRAARGGKGFRLPPLNQSIAPGEIVAIIGPNGA
                     GKTTLLKTLAGLLPPLAGSIARPRAAWLPPPGAVSAGFSVLHLTALGRSHLRGWSSGL
                     APADLDAAHAALDRLEVGHLAERPFDRLSSGQQQLVLIARLFVQDAPLCLLDEPLALL
                     DPAHARAVETAIRSLAGEGRIVIASTHHLPFAARADRVLSLGSDRAEMSAPDRALAPD
                     RIRPLFGVEVDGCPCCGGPLS"
     gene            4498..>5322
                     /locus_tag="VF686_00720"
     CDS             4498..>5322
                     /locus_tag="VF686_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8660358.1"
                     /translation="MSLARLVPAALALLLSAAPAMAQDAPRWSFAIHGGAGVIERESL
                     TAEQDVAYRAALKQALEAGSAVLANGGAALDAVQAAIQVMEDDPLFNAGRGAVFTAAG
                     RNELDAAVMNGTDLTAGAVAGLTITRHPIAAARAVMERSPHVMLIGQGADSFAASVGL
                     EQVDPSFFFTERRWQGLERALRQQNLPIPERPAGAPGPQAANDLPAPPLNERKFGTVG
                     AVALDTEGRLAAGTSTGGMTAKQWGRVGDVPVLGAGTYASNRDGCAVSATGDGEYYI"
BASE COUNT          736 a         1878 c         1882 g          826 t
ORIGIN      
        1 caggaacagt tcgcccgtcg tgtggaacat gtagagcaga cccagcagca cgatcggtag
       61 ctggaaggcc gagttggcca tgcccgcgcc ccagaccacc accatgaagc caaggcccac
      121 ctggagcagg ccgaggccga acttcatcgt cggattggga tccatgttgc gcttggccag
      181 gtaggcccac agcgccgcca tgatcggtgc gaacagcagg atgaagcccg cgttgaacga
      241 ctgggtctgg gcggcggtga tggtggcgtc gatccagaag ccggtcgggg tgatccctgc
      301 cgcggccagc tgcgccggcg tgcccacggt gacgcccagc aggttgaacg ccgtcggcgt
      361 caggctgagg tcgacgttac ggtcggcgaa caggttcagc gaggtgccgg cctgttcgaa
      421 gagggtgaag aagaccaccg agccgaagat cagcacgacg gccagcatca tccgctcacg
      481 ctgcgcccgc gtctggcaga ccttgatgat gacgtagagg atgaacagca gcgaggcgat
      541 cgtcgcggcc gtcagcacgg tgccgacgag ggcgttgcgc tggaccagga accagaccag
      601 cccgacgccg agcgtggcca gcagatagat cgaccattcg cggttcaggg ggccgatcat
      661 cggccgcttc agcatctccg ggttgggcgg ctcgcccttg ccttcgagca gcggcttgcc
      721 gagcatgaag accacccagc cgagggccat gccgaggccg gccaggccga agcccgccca
      781 ccagccgacg gtctgcccca gatagccgca cagcaccgcg gcccagaagg ccccgaggtt
      841 gatgccgtag tagtagaggg tgaagccgga atcgcgccgg gggtcgccct gcggatacag
      901 ctgtccgacg atggtcgaga tgttcggctt caggaagccc acgcccatga tgatcagcga
      961 cacggccaga tagaagacgt tgacgcccat caggtcgcgg gtctgggtca tcacgtacga
     1021 cccctcggcc agcgtagccg gcagaggcga tgcggccggc aggccggtca ccgcgatgcc
     1081 gcccgtttcg gccggaccga aggcgtattt ctggccgtcg acgacgatgt tgacgtcgcg
     1141 atcggcgccg cggccttcgg ccgaaatctc ataggtctga ccggcgtagg tcagggtttc
     1201 ggtggcggga cggccctcga aggccatcag gccgtgaccg gcgaccagca ggacggcgcc
     1261 gaaggcaatg gccttgcgcg tgccgatgaa gcggtcggcg aggatgccgc cgaccagcgg
     1321 cagcagatag accagcgagg tgtaggaccc gtaggtcgag cccgccatcg cgtcgtcgaa
     1381 caggaaatgc tgggtcagga agaagatcag gatgccgcgc atgccgtagt aggagaaccg
     1441 ctcccacatc tcggcgaaga acaggatgat cagtccgcgc ggatggttct tgaggatctg
     1501 catcagcacc gggatgccgg tggcgaaggt gatgatcagg cccacgatga taacgatgtt
     1561 catgcctggc ctcctgcggt ttgccgggct tcaagcccga tgcgccctgt aagcgcgccc
     1621 cctgcgtccg tcctactcaa cggctcactc ccttgttgct gcgccccacc tgacgcgcga
     1681 tgggcgcata agcagcacgc ggagcaagac cggacaagag ttaagggtaa attggttgac
     1741 ggtagcgccc ggggcggcgg aacggcacga tgctcgggat tcgcagcatg gggcggttat
     1801 gaccacgaca cgcaggtcag cgttaatcgg actcggcggc ggcctgaccc tttcggcctg
     1861 ttcgctcggg ccgtcgcggg acgactccgg gaaccgtgac gtcgtcgcgg ccggctcgcc
     1921 ggcggcgctg ctcgtctggg ccgtcgcgcg cgagcggctg gcgggctggc ctcgcaaacc
     1981 ggcgcagtcc gccctgtcgg ccctgcccgc tttcgccgcc ggcctgcctg aactgggggg
     2041 tctgacgggc aataaccggc acgcgaatct cgaggccgtc gctgcgctca agccgcggct
     2101 gatcatcgac tacggagaca tcgaccccga gtatcaggtc ctcgcccagc gcctgaaggt
     2161 ccagctgggc gccgactggc ggctgatcga tggagccctg tcccgaattc cggaggcgat
     2221 ccgtgaggcc ggagccctgc tgggagccgc gcccaaggcg gaccccctgg ccgacaacgc
     2281 cgccgaggtg ttggagcgct ggcgtcaggc gccgcccggc ccgagcttct actacgcccg
     2341 ggggggcgac gggctggaga cgggtttccg cggcgccttg gccaccgagg ttctggaagg
     2401 cgctgcctgg accaatgtcg ccgagggctc ccgcgacatc ggccgggtga cccgcgaaca
     2461 ggtcgccgcg tgggaccccg aggttctggt caccctcgat cgtggcttcg cggaatcggt
     2521 ccggagcgat cccgtctggc gctggcggcg gaacggcttg cggcggcgcc tgctgctgct
     2581 gcctgaccgg ccgttcggct ggatcgaccg gccgccgtcg gtgaaccggc tgcttgggtg
     2641 cgcctggctg gcggatccgc agggacccac tttgaccctc gccgccctca gccgccgtct
     2701 ttacggcatg gcgccggtcg agatcgccca gccgcgatgg atttcttgaa ccgccttagg
     2761 tcgtccctgc cggctctggc cctgaccctg ctcgcccttg cgtgcgcggc cggggtgctt
     2821 tccacgggcc cgctggcgat cccgccagcg gatcttctgt cggccctgat cggccaaggc
     2881 gggccggacg cggagctggt cctgaccctg cgcggaccgc gattgctggc cgccctgacc
     2941 tgcggcgcgg ctctggccgg agcgggcgcg gcgttccaga tcctgttccg caatccgctg
     3001 gcggcccccg acctgctggg agtctcgtcc ggcgcgggac tgggcgcggc cctggcgctg
     3061 cttctcggag cggggaccgt gctggtccag agcgcggcct tcgccggcgg cctcgcgatc
     3121 ggcggactgg ccttgggatg cgcggccctg acccgcaggg gcgacgggcg gctggccctg
     3181 atcctgtgcg gcgtcgtcgc cggagctctc gcctcggccg gactgggcct cgtggtcatc
     3241 gtcgccgagc cttattcgca gttgccggcc atcacctact ggctgctggg ctccttcacc
     3301 cgcgccaccc tggccgaggc gctgttcgcg cttgcgccga tctgcttcgg cggagccgtc
     3361 ctgctctggc tcggcttccg gctcgacgtc ctgtcgttgg gcgacgaaca ggcgcggtcg
     3421 ctgggcctcc cggcccgccg gctgcggctg acagctctgg cggcggcggc gctgatgacc
     3481 tctgcggctg tcgccttggc cggcatggtc ggctggatcg gccttctggc cccccacgcg
     3541 gcgcggctga tcgtcggcga gcgagcgggg cagctgatcc ccgcctcgat ggcgatcggc
     3601 gcgctgttcg ccctggttat cgaccggctg gccaccgcct tcggacccgc tgaaatcccc
     3661 gtcggcctgc tatccgccgc cctcggggcg cccgccttcc tgttcctttt cgtcgccgcc
     3721 tcgaggcggc catgagcagc ctgctgctgg agggtctggt cgccggacgc gccgcgcggg
     3781 ggggcaaggg gttccgcctg ccgccgctga accagagcat cgcccccggc gagatcgtcg
     3841 ccatcatcgg tccgaacggc gcgggcaaga ccaccctgct gaagaccctc gcgggcctgc
     3901 tgccgccgct ggcgggctcc atcgcccggc ctcgcgcggc ttggctgccg ccgccgggcg
     3961 cggtcagcgc gggcttttcg gtgttgcacc tgacggcgct ggggcggtcg cacctgcggg
     4021 gctggtcctc tggcctcgcc cccgccgatc tggacgccgc ccatgccgca ctggaccggc
     4081 tcgaggtggg ccatctggcg gaacgtccct tcgaccgcct ttccagcggt cagcagcagc
     4141 tggtcctgat cgcccgcctg ttcgtgcagg acgcgccatt gtgtttgctg gacgagccgc
     4201 tggccctgct ggaccccgcc cacgcccggg cggtcgagac cgcgatccgg tccttggccg
     4261 gagaaggccg catcgtcatc gcctcgaccc accacctgcc gttcgccgcg cgcgccgacc
     4321 gcgttctgtc gctcgggtcg gaccgcgcgg agatgagtgc gccggaccgc gccctggccc
     4381 ccgaccgcat ccgccccctg ttcggcgtcg aggtcgacgg ctgtccgtgt tgcggcggcc
     4441 ccttgagttg agcccgcgcc tgggcgaggt tccggccgtg tttctgaaag gctgaccatg
     4501 tccctcgccc gcctcgtacc ggccgccctc gcgctgctcc tgtccgccgc gcccgccatg
     4561 gcccaggacg cgccgcgctg gtcgttcgcc atccacggcg gcgccggcgt catcgagcgc
     4621 gaaagcctga ccgccgagca ggacgtcgcc tatcgcgcgg ccctgaagca ggcgctggag
     4681 gccggttcgg ccgtgctggc caacggcggc gcggcgctgg acgcggtgca ggccgcgatc
     4741 caggtcatgg aggatgatcc gctgttcaac gccgggcgcg gcgcggtctt caccgccgcc
     4801 gggcgcaacg agctggacgc cgccgtgatg aacgggaccg acctgaccgc cggcgccgtg
     4861 gccggactga ccatcacccg ccacccgatc gccgcggcgc gggcggtcat ggagcggtcg
     4921 ccccacgtca tgctgatcgg ccagggcgcc gacagcttcg ccgcctcggt cgggctggag
     4981 caggtcgacc cgtccttctt cttcaccgaa cgccgctggc aggggctgga gcgcgcgctg
     5041 cgccagcaga acctgcccat ccccgaacgc cccgctggcg cgcccggccc gcaggccgcc
     5101 aacgacctgc ccgccccgcc gctgaacgag cgcaaattcg gcaccgtcgg cgcggtggcg
     5161 ctggacaccg aaggccgact ggccgcgggc acatcgaccg gcggtatgac cgccaaacag
     5221 tggggccggg tcggcgacgt gccggttctc ggcgcgggga cctatgcgtc gaaccgcgac
     5281 ggctgcgccg tgtccgcaac gggcgacggc gaatattata tc
//
LOCUS       DASVVN010000043         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_159476,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000043 DASVVN010000000
VERSION     DASVVN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159476"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF686_00725"
     CDS             complement(<1..222)
                     /locus_tag="VF686_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX8660359.1"
                     /translation="MAPAPSVPSDNSRAALLSAFGCYVMWGFMPLLFMGQHAVGFDAL
                     EILAHRALWAVAVAGLLVFLAGQGGQVRAA"
     gene            complement(349..1395)
                     /locus_tag="VF686_00730"
     CDS             complement(349..1395)
                     /locus_tag="VF686_00730"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX8660360.1"
                     /translation="MGYRVAVVGATGNVGREMLNILEELTFPVEEMHAVASRKSKGVE
                     VSWGDKTLKCEDIELFDFSKVDLVLMSAGGDASRAWSEKIGAAGPMVIDNSSAFRMDP
                     DVPLIVPEVNPDAIKLASKKNIIANPNCSTAQLVVALKPLHDAAKIKRAVVSTYQSVS
                     GAGKAGMDELWNQTKAIYGLGDARPKLFPKQIAFNVIPFIGSFLDDGYTDEEAKMWKE
                     THKILDPDIKLTVTCVRVPVFVGHSESVNLEFDRPISPDEARAILRDAPGVMVIDKQE
                     HDGYITPVDAAGEHAVYVSRIRKDPTVENGLSLWVVSDNLRKGAALNAVQIAQLLDET
                     GMIKPASGYRSITV"
     gene            complement(1557..2480)
                     /locus_tag="VF686_00735"
     CDS             complement(1557..2480)
                     /locus_tag="VF686_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660361.1"
                     /translation="MRFLTGAAFCIALGCSTPASAQDTVPLPVRIEGPAHIRLHITKT
                     DRRDAGPEQGAAFTYDVTLSKAQEDTGRREIVWRLTHVDGEGVTPESSPGPDIRMTVD
                     ETLTPLSLDNLGEIIASTRRQLERSVDLEASGGALRMLASLTPETAAPLFMADAGMIA
                     VGQGTDLFLGEDSSYEAVGRLPWGGADIIMIGTYRLVEVDRATGKARLQWSQEIDPDS
                     LMEAIPAMIDGIVADKVKQDDSADMAAKIRASLTNATLRNNRSCDFMIDIATGLAEKV
                     DCLTLVEFVAGEETSRRETRLIATQVLTPRS"
     gene            2544..3656
                     /gene="zapE"
                     /locus_tag="VF686_00740"
     CDS             2544..3656
                     /gene="zapE"
                     /locus_tag="VF686_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX8660362.1"
                     /translation="MPSRTSRAYQNRIQDGRLTPDPSQVVVVEALSRLEKDLRKKAPL
                     GGLFGQNEVRGVYLWGPPGRGKSILMDLFYACAPEPRKTRAHFHAFMARVHDLIGQWR
                     RGDAKTRKAVFGQSKGDDPIRPVAELIASEARLLCFDELQVTDIADALILGRLFEALF
                     EQNVVLAVTSNRAPDQLYLNGINRELFVPFIDLIEEKCAVVSVSGSRDWRLDRLMGDK
                     VWFAPADEASRAAFEALWSDLKGDQEECPAHLKVQGRDVVIERTDGGLARATFAQLCG
                     TALGPQDYLAIAERFHTLFVEDAPVLGPANHHEARRFVTLIDALYEAGTRLVVLAEAA
                     PEALYQKGVGAFEFERTVSRLNEMSGAEWLARERTS"
     gene            3730..4245
                     /locus_tag="VF686_00745"
     CDS             3730..4245
                     /locus_tag="VF686_00745"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8660363.1"
                     /translation="MRLSVLFAVLALGAASPVLAQTPATPVASKGVVVADTRTWLIGL
                     GGSVAEPAAENGAQVLTIADQPLPWTLTFYNCAGANLCDDAQYSAVFSGPITVEQINE
                     WNRGNRYLKAFFVPGATPDAAGAIAQYDVVLTSTGTEQLQEPTVIWLQMLRQFAQSLA
                     QAAGVTAPPAQ"
BASE COUNT          723 a         1410 c         1555 g          796 t
ORIGIN      
        1 ggccgcgcgg acctgaccgc cctgcccggc gaggaagacc agcaggccgg caaccgccac
       61 ggcccacagg gcgcgatggg cgaggatctc cagcgcgtcg aagccgaccg cgtgctggcc
      121 catgaacagc aggggcatga agccccacat gacatagcag ccgaaggcgg acagcagggc
      181 ggcgcgggaa ttgtcggacg gaacggaggg ggcgggcgcc atggcggctt cctgagccgt
      241 cattcccggg ccgcgaagcg ggacccggaa cccaggaggg cggatgcggt cctgggttcc
      301 ggatcaggcc ttcggcctgt ccggaatgac gatgagatag ggaggaaatc agacggtgat
      361 cgagcgatac cccgaggccg gtttgatcat gcccgtctcg tccagcagct gggcgatctg
      421 gacggcgttg agggcggcgc ccttgcgcag gttgtcggag acgacccaaa gggacaggcc
      481 gttctcgacg gtcgggtcct tgcggatgcg cgagacgtag acggcgtgtt cgccggcggc
      541 gtcgaccggg gtgatgtagc cgtcgtgctc ctgcttatcg atgaccatga cgccgggggc
      601 gtcgcgcagg atggcgcggg cctcgtcggg cgagatcgga cggtcgaact cgaggttcac
      661 agactcggag tggccgacga agaccggcac gcgcacgcag gtgacggtca gtttgatgtc
      721 gggatcgagg atcttgtgcg tctccttcca catcttggct tcctcgtcgg tgtagccgtc
      781 gtcgaggaag gacccgatga aggggatgac gttgaaggcg atctgtttgg ggaacagttt
      841 cggccgcgcg tcgccgaggc cgtagatggc cttggtctgg ttccacagct cgtccatgcc
      901 tgccttgccg gcgcccgaga ccgattgata ggtcgagacg acggcgcgtt tgatcttcgc
      961 cgcgtcatgc aggggcttga gggcgacgac cagctgggcg gtcgaacagt tggggttggc
     1021 gatgatgttc ttcttcgacg ccagcttgat ggcgtcgggg ttcacctcgg gcacgatcag
     1081 cggcacgtcc gggtccatgc ggaaggcgga ggagttgtcg atgaccatgg ggccggcggc
     1141 gccgatcttt tccgaccagg cgcgcgaggc gtcgccgccg gcactcatca gcaccagatc
     1201 gaccttcgag aaatcgaaca gttcgatgtc ctcgcatttg agggtcttgt cgccccagga
     1261 cacctcgacg cccttggact tgcgcgaggc gacggcgtgc atttcctcga cggggaaggt
     1321 gagctcctcg aggatgttca gcatttcccg accgacattg ccggtggcgc ctacgacggc
     1381 gacgcgatag cccattttgg ttggtcctgt tttgagttcg gggccgcgtt tcgcgcaact
     1441 cgggcgcgga tttgtggcga gcctctatgt ggggcttccc ggcggggacg caatcgcttt
     1501 tccgccggcc cgccgccgct cagcccgaga tcggacggcc tggtcatttg ccgcgcttat
     1561 gaccgcggcg tcaggacttg cgttgcgatc agccgggtct ctcttcgcga cgtctcctcg
     1621 ccggcgacga attcaacgag ggtcagacag tcgaccttct cggccagacc cgtagcgata
     1681 tcgatcatga agtcgcagct gcggttgttc cgcaacgtcg cattggtcag ggacgcccgt
     1741 atcttggccg ccatgtcggc gctgtcgtct tgcttgacct tgtctgcgac tatcccgtcg
     1801 atcatggccg gaatggcctc catcagggag tcgggatcga tctcctgcga ccattgaagc
     1861 cgtgcctttc cggttgcccg atcgacctca acgagccggt aggttccgat catgatgatg
     1921 tcagccccgc cccatggaag ccgccctacc gcctcgtagg agctgtcctc tcccagaaaa
     1981 aggtctgttc cctgcccgac ggcgatcatc cccgcgtcgg ccatgaagag gggcgccgcc
     2041 gtttccggcg tcaggctggc cagcatgcgc agagcgccgc cgctcgcttc cagatcaacc
     2101 gaccgctcaa gttgccggcg cgtcgacgca atgatttcgc cgaggttgtc cagggagagc
     2161 ggcgtcagcg tctcgtcgac ggtcatccgg atgtccgggc cgggcgacga ctctggcgtc
     2221 acgccctcac cgtcgacatg ggtgaggcgc cagacgatct cgcggcggcc ggtgtcttcc
     2281 tgcgccttcg aaagagtgac gtcgtaggtg aaggcggccc cctgttccgg cccggcgtcc
     2341 ctgcggtccg tcttggtgat gtgcagccgg atatgcgccg gaccttcgat gcggaccggc
     2401 aggggcacgg tgtcctgcgc ggacgctggc gtggagcatc ccagcgcaat acaaaacgcc
     2461 gcgcctgtca gaaaccgcat gagtcactcc cctgtgctcc aacctagcta ggcggcgggc
     2521 gcgaggaaag gctatgaggc cgtatgccgt cccgaaccag ccgcgcctat caaaaccgga
     2581 ttcaggacgg ccggctgacc cccgatccgt cgcaggtcgt ggtggtcgag gcgctgtcgc
     2641 ggctggagaa ggatctgcgc aagaaggccc cgctgggcgg gctgttcggc cagaatgagg
     2701 tgcgcggcgt ctatctgtgg gggccgccgg ggcggggcaa atccatactg atggacctgt
     2761 tctacgcctg cgcgcccgag ccgcggaaga cgcgggccca tttccacgcc ttcatggcgc
     2821 gggtccacga cctgattggc cagtggcgca ggggcgatgc gaagacgcgc aaggcggtct
     2881 tcggtcagtc gaagggcgac gaccccatca gacctgttgc ggagctgatc gcgtccgagg
     2941 cgcggctgct gtgtttcgac gaactgcagg tcaccgacat cgccgacgcc ctgatcctgg
     3001 gtcggctgtt cgaggcgctg ttcgagcaga atgtggtgct ggcggtgacc tcgaaccggg
     3061 cgccggacca gctgtatctg aacgggatca accgcgaact gttcgtgccc ttcatcgacc
     3121 tgatcgagga gaaatgcgcc gtggtcagcg tctcggggtc gcgcgattgg cggctggacc
     3181 ggctgatggg cgacaaggtg tggttcgcgc ctgcggacga ggcctcccgg gccgcgttcg
     3241 aggcgttgtg gtccgacctc aagggcgatc aggaggagtg tccggcgcat ctgaaggtgc
     3301 agggccgcga cgtggtcatc gagcgcaccg acggcggact ggcgcgagcg acctttgcgc
     3361 agctgtgcgg aacggcgctg gggccgcagg actatctggc catcgccgag cggtttcaca
     3421 ccctgttcgt cgaggacgcg ccggtgctgg gcccggccaa tcaccacgag gcgcggcggt
     3481 tcgtgaccct gatcgatgcg ctgtacgagg ccggaacccg gctggtggtg ctggccgagg
     3541 cggcgcccga agccctgtat caaaagggtg tcggcgcctt cgaattcgag cggacggtgt
     3601 cgcgcctgaa cgaaatgtcg ggcgcggagt ggctggcgcg cgaaagaacg tcgtaaggtg
     3661 gccgccacaa ttccgctggg cccgcccgac catcatcggg cctacagttc gcgatcccgg
     3721 agatttccca tgcgcctgtc cgtcctgttc gcagtcctcg ccctcggcgc cgcctcgccg
     3781 gtcctcgccc agacgcctgc cacgcccgtc gccagcaagg gggtcgtcgt cgccgacacc
     3841 cggacctggt tgatcggact gggcggttcg gtggccgagc cggcggcgga gaacggggcg
     3901 caggtcctga ccatcgccga tcagccgctg ccgtggaccc tgaccttcta caactgcgcg
     3961 ggcgcgaacc tgtgcgacga cgcccagtac agcgcggtct tctcgggccc gataacggtc
     4021 gagcagatca atgagtggaa ccgcggcaac cgctatctga aggccttttt cgtgccgggc
     4081 gccacgcccg atgcggccgg cgccatcgcc cagtatgacg tggtcctgac ctcgacgggg
     4141 acggaacagc tgcaggaacc gacggtgatc tggctgcaga tgctgcggca gttcgcgcag
     4201 agcctggcgc aggccgcagg cgtgactgcg ccgccggcgc aatagacgcg gctattgtgt
     4261 ggagtggcgg gccctgcggc gcatcctcgg aggggcgccg aaggccacgt ccatcagtct
     4321 gatctcggcg acctgccgga tcgcacccac aacccacgcc atcatttctc tctgaagtta
     4381 agctaacccg ttgaaaccat gatggtccgg cgcagccggc aattcattaa cttcggttag
     4441 ctttcggcga accgcgccct gtgaactctt gatcgtgcgc acga
//
LOCUS       DASVVN010000044         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_212003,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000044 DASVVN010000000
VERSION     DASVVN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212003"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..>1691
                     /gene="ubiA"
                     /locus_tag="VF686_00750"
     CDS             574..>1691
                     /gene="ubiA"
                     /locus_tag="VF686_00750"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX8660364.1"
                     /translation="MTSAPLPDAGSNWVDRHAPEGLKPWLKLGRFDRPVGIWLLLLPG
                     WQGIALALAQYSKTPDLYDAWLFAGFALGACLMRAAGCAFNDIVDRDIDAKVSRTAMR
                     PIPSGRISVKQAWAFVIGCSLISLLILLTLNWTAILLGFGSVLLVAAYPFMKRITWWP
                     QAWLGLTFNWGALMGFAAAATAWVSFLLSDASTVALGVRAGYLTTAAVFLYLGGVFWT
                     LGYDTIYALQDIEDDAMVGVKSSARRLGSNVPRGVAVFYGLAVACAAVAGWLAGLGPL
                     FWPGLAAYGLHLASQDRRIRTDDPALALRLFKSNRDAGLPGHLTPAAVLLYLGGVFWT
                     LGYDTIYALQDIEDDAMVGVKSSARRLGSNVPRGVAIF"
BASE COUNT          232 a          626 c          523 g          310 t
ORIGIN      
        1 cttcgaacgc cccggaatga cggtgtggat gctgcaggtt cgcgctgaaa cagacgagtt
       61 gcagaagcca acctgctgac ctttcactgg atttcagaac gaccagcgtc atttcgtagc
      121 cttggttaag acccgtgcca cacttctccc atcccgtcgg tggggagggc gtcggatcca
      181 cgcttctcga tgtcggcggg ctgtggtcga gcgatgaagc ccgggggagg cagacctcgg
      241 gggtccttag gcccagcgat ggatgctggg ccctgctcgc cgcgcgcttc tcgtgcgggg
      301 aatctctggg gctatacgcc ttaatgtccc cctcccggtc cgctccgcaa ggcgcagtcc
      361 gggtgtcggc aaggccagac aacggccggc ggccgacggg cgcgtggaaa acgacgacgc
      421 ggggcggctg tctcgtcgaa acggtatttc tattccagca ccgggcgagc ccggtcgccc
      481 cgcgccctcc ccgaactttt ccgcggaaac gctgaagagc ctggacgcat gcctgtactt
      541 ctcccctccc ctccgacagg ctatctccga cgcatgacct ccgccccgct tcccgacgcc
      601 ggatcgaact gggtggaccg ccacgcgccg gaaggcctca agccgtggct caagctcggc
      661 cgcttcgacc gccccgtcgg catctggctg ctgctcctcc ccggctggca ggggatcgcc
      721 ctcgctctgg cccagtacag caagacgccg gatctgtatg acgcctggct gttcgccggg
      781 ttcgcgctcg gcgcctgcct gatgcgggcc gccggctgcg ccttcaacga catcgtcgac
      841 cgggacatcg acgcgaaagt gtcccgcacg gccatgcgtc ccatcccgtc cggtcgcatc
      901 agcgtcaaac aggcgtgggc ctttgtgatc ggatgcagcc tgatcagcct gctgatcctg
      961 ctgaccctca actggacggc catccttctc ggcttcggat cggtcctcct cgtcgcggcc
     1021 taccccttca tgaagcggat cacatggtgg ccccaggcgt ggctcggcct gaccttcaac
     1081 tggggcgcgc tgatgggctt cgccgccgcc gcgacggcct gggtgtcatt tctgctgtcg
     1141 gacgcatcga ccgtggccct cggcgtgcgg gcgggctacc tcaccaccgc cgccgtgttc
     1201 ctctacctcg gcggcgtctt ctggaccctc ggctacgaca ccatctacgc cctgcaggac
     1261 atcgaggacg acgccatggt cggggtcaaa tcctcggccc gccgcctcgg atcgaacgtg
     1321 ccgcgcgggg tcgcggtctt ctacggcctc gccgttgcct gcgccgccgt cgccggatgg
     1381 ctggccggcc tcggcccgct gttctggccc ggtctcgccg cctatgggct gcacctcgcc
     1441 tcccaggatc ggcgcattcg cacggacgac cccgccctcg ccctgcgcct gttcaagtcc
     1501 aatcgcgacg ccggcctgcc cggccatctc acccccgccg ccgtcctcct ctacctcggc
     1561 ggcgtcttct ggaccctcgg ctacgacacc atctacgccc tgcaggacat cgaggacgac
     1621 gccatggtcg gggtgaagtc ctcggcccgc cggctcggat cgaacgtgcc gcgcggggtc
     1681 gcgatcttct a
//
LOCUS       DASVVN010000045         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_80516, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000045 DASVVN010000000
VERSION     DASVVN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80516"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..72
                     /locus_tag="VF686_00755"
     CDS             <1..72
                     /locus_tag="VF686_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HEX8660365.1"
                     /translation="FSLGADGKEGGEGNDADLGNWQG"
     gene            41..511
                     /locus_tag="VF686_00760"
     CDS             41..511
                     /locus_tag="VF686_00760"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX8660366.1"
                     /translation="MTPIWATGRVEARTHRAGFTLVELLMVVAIVGLAAGAVVLAVPD
                     PRPPVGAEAERFAARLSRAREEAVLSNRPVAVEVTPTGYAFTAFDGRAWSPLSDGPFG
                     PETWRTETAVAPDEGARFVFDPTGVAEPVTLTLARDGKRRTVSVDGAGEVRIDD"
     gene            504..860
                     /gene="gspI"
                     /locus_tag="VF686_00765"
     CDS             504..860
                     /gene="gspI"
                     /locus_tag="VF686_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX8660367.1"
                     /translation="MTEHSGFTLIEMLVAMAVFGLAAMALLNLSGENTRSAARVETRT
                     LGGVVAENLAVEAATLPSIGEGTASGQIDLAGRRWTWTRAVTATDVAGMLRIDVRVSD
                     AEGQAAERTLFRARTA"
     gene            857..1447
                     /gene="gspJ"
                     /locus_tag="VF686_00770"
     CDS             857..1447
                     /gene="gspJ"
                     /locus_tag="VF686_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEX8660368.1"
                     /translation="MTRRAGFTLVEVLISLMIFAMIAAAGAAVLGVTIDNRFAVKAAS
                     DRVGDLQRMRSLMRADLGQATARRSRGTTGRPMPQPMVGAATPGDPILTLTRAGWSNP
                     GEQARPSLQRVEYRLVEDRLERRVSSHLDGARPGPPQVLYRGVRDVTVAFVRDGQAAP
                     AFISSIDRPLPDAVSIGMTVQGYGRIEQLFLVGSGG"
BASE COUNT          198 a          548 c          551 g          221 t
ORIGIN      
        1 ttctcgcttg gcgccgacgg caaggagggg ggcgagggca atgacgccga tctgggcaac
       61 tggcagggtt gaggcccgga cccatcgcgc gggcttcacc ctggtggagc tgctgatggt
      121 ggtcgcgatt gtcggtctcg ccgccggcgc ggtggtgctg gcggtcccgg atccgcgtcc
      181 gcccgtcggc gcggaggccg aacggttcgc agcccggctg tcccgcgcgc gcgaagaggc
      241 cgtgctgtcc aaccgcccgg tcgcggtaga ggtgacgcca accggttacg ccttcaccgc
      301 cttcgacggc cgcgcatggt cgcccctgtc cgacggtccg ttcgggccgg aaacctggcg
      361 gacggagacc gccgtcgcgc cggacgaagg cgcccgcttc gttttcgatc cgacgggcgt
      421 ggccgaaccg gtgacgctga cgcttgcccg cgacggcaag cgccgcaccg tgtccgtcga
      481 cggcgccgga gaggtgcgga ttgatgactg agcattccgg gttcaccctg atcgaaatgc
      541 tggtcgccat ggccgtgttc ggcctggcgg ccatggcctt gctgaacctg tcgggcgaga
      601 acacccgctc agccgctcgc gtcgaaaccc gcaccctggg cggcgtcgtg gccgaaaatc
      661 tcgcggtcga ggccgccacc ctgccgtcga tcggggaggg gaccgcctcg ggccagatcg
      721 acttggccgg ccgccgctgg acctggaccc gcgccgtcac ggccacggac gtcgccggca
      781 tgctgcgcat cgacgtccgg gtcagcgacg ccgaggggca ggccgccgaa cgcaccctgt
      841 tcagggcgcg aaccgcatga cccgccgcgc cggcttcacc ctggtcgagg tcctgatctc
      901 gctgatgatc ttcgccatga tcgcggcggc gggcgcagcg gttctcggcg tgaccatcga
      961 caaccggttc gcggtcaagg ccgccagcga ccgcgtcggc gacctgcagc gcatgcgcag
     1021 cctgatgcgg gcggatctgg gccaggccac cgcccgccgc tcgcgcggga cgaccggccg
     1081 gcccatgccc cagccgatgg tcggagccgc cacgccgggc gatccgatcc tgaccctgac
     1141 ccgcgcgggc tggagcaacc ccggcgaaca ggcgcggccc tccctgcagc gggtcgaata
     1201 ccggctcgtc gaggaccggc tggagcggcg cgtctccagc catctggacg gcgcccggcc
     1261 cggcccgccg caggtgctgt accgcggcgt gcgcgacgtc accgtcgcct tcgtccgcga
     1321 cggacaggcg gccccggcct tcatctccag catcgaccgt ccgctgcccg acgccgtgag
     1381 catcggcatg acggttcagg gctatggccg gatcgaacag ctgttcctcg tcgggagcgg
     1441 cgggtgagcc cgtcacgcgg ggatcgcgag ggcatggccc tgctgacggt gctgctgctg
     1501 gtcgccgtca tgtcggtg
//
LOCUS       DASVVN010000046         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_343623,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000046 DASVVN010000000
VERSION     DASVVN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343623"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF686_00775"
     CDS             complement(<1..277)
                     /locus_tag="VF686_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8660369.1"
                     /translation="MISNRSVRFGARALALVGVALSLPACATITRGSSQEFTVTSEPP
                     GARVSTSNGFQCDATPCTFRMPRKDEFRATVSMDGYATQTHEIQSGIS"
     gene            complement(377..973)
                     /locus_tag="VF686_00780"
     CDS             complement(377..973)
                     /locus_tag="VF686_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8660370.1"
                     /translation="MKLYVTAPSPFARKCRIVAREKGLMDRIEEIMVDPYANDPELLA
                     ANPIVQAPTLIAGDGLPVSDSPVICEYLDAIGAGPRLLPAEGADRLRVRRMETLANGA
                     LEMGVKLLLEIRRPEDERSPSWMARWAANMARALDALETARPDAARIDMGVITAGCAV
                     TWIGFRHPDFDWRGGRPHLVALHEVIETRPSFAETRPG"
     gene            complement(999..2189)
                     /gene="ispG"
                     /locus_tag="VF686_00785"
                     /gene_synonym="gcpE"
     CDS             complement(999..2189)
                     /gene="ispG"
                     /locus_tag="VF686_00785"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX8660371.1"
                     /translation="MALSSRHAYIVPVMSDHSHIRPWRHIERRKSRQIMVGSVPVGGD
                     APISVQSMTNTPTSDAAATIDQIRQLEEAGADIVRVSCPDEESTAAFRQIAREAKVPL
                     VADIHFHYKRGIEAAGAGAACLRINPGNIGSPDRVREVIQAAKDHGCSMRIGVNAGSL
                     ERELLEKYGEPCPEAMVESALNHARILQDHDFHEFKISVKASDPFLTVAAYQQLAEAI
                     DCPLHLGVTEAGPLRSGTIKSAIGMGNMLWAGIGDTIRVSLAADPVEEIKVGFDILKS
                     LGLRHRGVNIIACPSCARQGFNVIETVGILEEKLAHVTTPMSLSIIGCVVNGPGEALY
                     TDVGFTGGGKGAGMVYLNGKMAHKQANEGMIDEIVHLVEQKAAELDAARAAQAPAPVL
                     EPAE"
BASE COUNT          348 a          686 c          819 g          365 t
ORIGIN      
        1 ccgaaatgcc gctctggatc tcgtgcgtct gggtcgcata gccgtccatc gagacggtgg
       61 cgcggaactc gtccttgcgg ggcatgcgga aggtgcaggg cgtcgcgtcg cactggaagc
      121 cgttggaggt cgagacgcgg gcgcccggag gctcgctggt gacggtgaac tcctggctgg
      181 agccgcgcgt gatggtggcg caggccggca gcgagagcgc gacgccgacg agcgcgagcg
      241 cgcgcgcgcc gaagcgcacg gaacggttgg aaatcatgaa agcccccaaa ggatacgggc
      301 ccaccccccg gcggacctga aatctgtttt gcatcagggg gcggcgtcgt caatcaccat
      361 accgtgatgc atgggttcaa ccgggccggg tttccgcgaa gctcggccgg gtctcgatca
      421 cctcgtgcag ggcgaccaga tgcggccggc cgccacgcca gtcgaagtca ggatggcgga
      481 agccgatcca ggtcacggcg cagccggccg tgatgacgcc catgtcgatc cgggcggcgt
      541 cgggccgggc cgtctcgagc gcgtcgaggg cgcgggccat gttggcggcc cagcgagcca
      601 tccacgacgg cgagcgctcg tcttcgggcc ggcggatctc gagcagcagt ttgacgccca
      661 tctcgagcgc gccattggcc agggtctcca tccgccgcac ccgcaggcgg tcggcgcctt
      721 cggcgggcag cagacggggg ccggcgccga tggcgtccag atattcgcag atcaccgggc
      781 tgtcgctgac cggcaggccg tccccggcga tcagggtcgg ggcctggacg atgggattgg
      841 cggccagaag ctcggggtca ttggcgtagg gatcgaccat gatctcctcg atccggtcca
      901 tcagaccctt ttcgcgcgcg acgatgcggc atttgcgggc gaagggagag ggggcggtga
      961 cgtagagctt catggtcgcc tttgcagcgg acgccggact attcggcggg ttcgaggacc
     1021 ggagccgggg cctgggccgc gcgggcggcg tccagttcgg cggccttctg ttcgaccaga
     1081 tggacgatct cgtcgatcat gccctcattg gcctgtttgt gggccatctt gccgttcaga
     1141 tagaccatgc ccgcgccctt gccgccgccg gtgaagccaa cgtccgtgta cagagcttcg
     1201 ccgggtccgt tgacgacgca gccgatgatc gacagcgaca tgggcgtggt cacatgggcc
     1261 agcttctctt cgaggatgcc gacggtctcg atgacattga agccctgacg ggcgcaggac
     1321 gggcaggcga tgatgttgac gccacggtgg cgcaggccca gcgacttgag gatgtcgaaa
     1381 ccgaccttga tctcttccac cggatcggcc gccagcgaga cgcggatggt gtcgccaatg
     1441 ccggcccaca gcatgttccc catgccgatg gcggacttga tggtgcccga gcgcaacggg
     1501 cccgcttcgg tgacgccgag gtgcaacggg cagtcgatgg cctcggccag ctgctgatag
     1561 gcggcgacgg tcaggaaggg gtcggaggcc ttcaccgaga tcttgaactc gtggaagtcg
     1621 tggtcctgca ggatgcgggc gtggttgagg gcgctctcga ccatggcctc ggggcagggc
     1681 tcaccgtatt tttcgagcag ttcgcgctcc agcgagccgg cgttgacgcc gatccgcatc
     1741 gagcagccgt ggtccttggc ggcctggatg acctcgcgga cccggtcggg cgagccgatg
     1801 ttgcccggat tgatgcgcag gcaggcggcg cccgccccgg cggcctcgat gccgcgcttg
     1861 tagtggaagt ggatgtcggc gacgagcggg accttcgcct cgcgcgcgat ttgacggaag
     1921 gcggccgtgg attcctcgtc ggggcaggag acgcggacga tgtcggcccc ggcctcttcc
     1981 agctggcgga tctggtcaat cgtcgccgcc gcatccgagg tcggcgtatt ggtcatcgac
     2041 tggacgctga tcggcgcatc gccgcccacg ggcacggagc cgaccatgat ctggcgggac
     2101 ttccggcgct cgatatggcg ccagggacgg atgtgggaat ggtcgctcat gacgggaaca
     2161 atataggcgt gccgggaaga caaggccatg acggccgctg ggggatcagc ggaccgtc
//
LOCUS       DASVVN010000047         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_317226,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000047 DASVVN010000000
VERSION     DASVVN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317226"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1614
                     /locus_tag="VF686_00790"
     CDS             169..1614
                     /locus_tag="VF686_00790"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX8660372.1"
                     /translation="MTETMNPTAALDRLEELYNQSVSNLRTAVGTFLRTGERADPEAR
                     AKGLFSYPELRVSWFGERPANLEQRAFARMSRPGVYSTTITRPDLYRPYLLEQLGLLE
                     ADYGATFDVRPSEQEIPFPYVMDGSDVALDRSQTAAVARYFPTTDLAHIGDEISDGLF
                     DLNKDFPLAQFDGLRTDFSLARLRHYTGTPVEDVQSYILFTNYNRYVDEFVRWACAQI
                     ADPNSAYEKLVCAGGIEITRDTPNPETAVMDAAWKKHQMPAYHLTAPGWKGITLVNIG
                     VGPSNAKTICDHLAVTRPHAWMMIGHCGGLRPSQSIGDYVLAHAYLRDDHVLDAVLPP
                     DIPIPSIAEVQRALYDAAKIVSGAPGEEVKRRLRTGTVVTTDDRNWELRYSKSALRFN
                     QSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGSISEHL
                     QIGIHAIDLLRAEGNRLHSRKLRAFDEPPFR"
     gene            complement(1753..2256)
                     /locus_tag="VF686_00795"
     CDS             complement(1753..2256)
                     /locus_tag="VF686_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEX8660373.1"
                     /translation="MTQIDKALKTGRRLTRQAAPAANAALSGGEMLQAAGDVINARLG
                     IMAAGLADPRKVDLAEMSLMSTEKVEALSASASAMAKTFGDIGGRVGSSALTEAGLAS
                     KAAASIAAASSPAAAMQAQYSYAIGWWSRAAGQMLTLNSELLKGQADAMRPIHRTAVA
                     NAKRLKK"
     gene            complement(2277..>2693)
                     /locus_tag="VF686_00800"
     CDS             complement(2277..>2693)
                     /locus_tag="VF686_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8660374.1"
                     /translation="NVATIEATFAEIYAEWSFVEEQPPFVLKLKNWWDLPGVHSLCTR
                     MFPTGHGMHATPSEIAVTQAAYPDRIKTADYSPQIAPSGPIRDALDYRARFPDGRIGS
                     DPAQASPEKGQTIIDAAVPALLKDVADFSAEVLPGV"
BASE COUNT          427 a          948 c          878 g          440 t
ORIGIN      
        1 tgtgactgcc ttcctgcatc gcgagttggg gtagcaatcg atcttcgcgc gagccgcgct
       61 caagccgcga gcgaccgcgt cgcgagcata gcgcccaaaa gagccaggcc acgcgaagcg
      121 tggccgtggc cgtctcgcca aggtcatgat cttccgctag aggaggccat gacagaaacg
      181 atgaacccaa cggcggcgct ggaccgtctc gaagaactct acaaccagtc cgtctccaac
      241 ctgcgcactg cggtggggac cttcctgcgg accggcgaac gcgccgatcc cgaggcccgg
      301 gccaagggcc tgttctccta tcccgaactc agggtcagct ggttcggcga acggccggcc
      361 aatctggagc agcgcgcctt cgcccgcatg tcgcgtcccg gcgtctattc gaccaccatc
      421 acccggccgg acctgtaccg tccctatctg ctcgaacagc tgggcctgct ggaggccgac
      481 tatggcgcca ccttcgacgt gcgcccgtcg gaacaggaga tccccttccc ctatgtgatg
      541 gacggctcgg acgtcgccct ggaccgcagc cagacggcgg ctgtagcgcg ctatttcccg
      601 accacggatc tggcccacat cggcgacgag atttcggacg gcctgttcga tctgaacaag
      661 gacttcccgc tggcccagtt cgacggcctg cgcaccgact tctcgctggc ccggctgcgc
      721 cactacaccg gcacgccggt cgaggacgtg cagagctaca tcctgttcac caactacaac
      781 cgctacgtcg acgagttcgt gcgctgggcc tgcgcccaga tcgcggaccc gaacagcgcc
      841 tatgagaagc tggtctgcgc cggcggcatc gagatcaccc gcgacacgcc caatcccgag
      901 acggcggtca tggacgcggc ctggaagaag caccagatgc ccgcctatca cctgacggcg
      961 ccgggctgga agggcatcac cctggtcaac atcggcgtcg gcccgtccaa cgccaagacc
     1021 atctgcgacc acctcgccgt gacccgtcca cacgcctgga tgatgatcgg ccactgcggc
     1081 ggcctgcgcc cctcgcagag catcggcgac tatgtgctgg cccacgccta tctgcgcgac
     1141 gaccacgtgc tggacgcggt cctgccgccc gacattccga tcccgagcat cgccgaggtg
     1201 cagcgcgccc tctacgacgc cgccaagatc gtctccggcg cgccgggcga ggaggtcaag
     1261 cgccgcctgc gcaccggcac ggtggttacc accgacgacc ggaactggga gctgcgctat
     1321 tcgaagtcgg cgctgcggtt caaccagtcg cgcgccgtgg ccatcgatat ggaaagcgcc
     1381 accatcgcgg cccaaggcta tcgcttccgc gtgccctacg ggacgctgct gtgcgtctcg
     1441 gacaagccgc tgcacggcga gatcaagctg ccgggtcagg ccaaccgctt ctacgagggc
     1501 tcgatctcgg agcatctgca gatcggcatc cacgccatcg atctgctgcg cgccgagggc
     1561 aaccgcctgc attcgcgcaa gctgcgcgcc ttcgacgagc cgccgttccg gtaaggtcgc
     1621 gccggcgttc tccctcccct ttagggggag ggtggccgcg aagcggccgg gtggggaatt
     1681 gtccagccgt accccaccct gtcgtcgctt cgcgccgaca tccctcccca tgaaggggag
     1741 ggagattccc tgttacttct tcagccgctt cgcattggcg acggcggtcc tgtggatcgg
     1801 gcgcatggcg tcggcctggc ccttcagcag ttcgctgttg agggtcagca tctggccggc
     1861 cgcccggctc caccaaccga tggcgtagct gtactgggcc tgcatcgcgg ccgcggggct
     1921 cgaggccgcg gcgatagacg ccgccgcctt cgaggccagt ccggcctcgg tcagggcgct
     1981 ggagccgacg cggccgccga tgtcgccgaa ggtcttcgcc atggccgagg ccgaggccga
     2041 cagggcctcg accttctcgg tgctcatcag cgacatctcc gccagatcga ccttgcgcgg
     2101 atcggccaga cctgcggcca tgatgccgag tcgggcgttg atcacgtcgc cggcggcctg
     2161 cagcatctcg ccgccggaaa gcgcggcgtt ggcggccggg gccgcctgac gggtcaggcg
     2221 gcggccggtc ttcagggcct tgtcgatctg ggtcatgaac cttgcctgga cctctttcag
     2281 acgcccggca agacctcggc ggagaagtcg gccacatcct tcaacagggc cgggaccgcc
     2341 gcgtcgatga tggtctggcc cttctcggga ctggcctgcg ccgggtccga gccgatgcgg
     2401 ccgtcaggga agcgggcgcg atagtccagc gcgtcgcgga tcggcccgct gggcgcgatc
     2461 tgcgggctgt agtcggccgt cttgatgcga tccggatagg ccgcctgggt aaccgcgatc
     2521 tcggacgggg tcgcgtgcat gccgtggccg gtcgggaaca tgcgggtgca caggctgtgg
     2581 acgcccggca gatcccacca gttcttcagc ttcagcacga agggcggctg ctcctcgacg
     2641 aagctccact cggcgtagat ttccgcgaag gtcgcctcga tggtggcgac gtt
//
LOCUS       DASVVN010000048         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_475154,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000048 DASVVN010000000
VERSION     DASVVN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475154"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..165
                     /locus_tag="VF686_00805"
     CDS             28..165
                     /locus_tag="VF686_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX8660375.1"
                     /translation="MDLHETAAKGLYVLIALHILGALKHQFIDRDNVVHRMILWIPRR
                     P"
     gene            162..716
                     /locus_tag="VF686_00810"
     CDS             162..716
                     /locus_tag="VF686_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8660376.1"
                     /translation="MKLLAALLVALLAPLLIGAAPGPWTFEREASRIEMSVRALGATR
                     TGQFEDWRGSLVLDPARPEEARGSVTVQAASLKMSPAVAASRAIGPSFLDAARHPTIR
                     LDLKSLEPLGGSRYTARADITVKGRTRAIAFPLDLRINGERAHLAGALTLDRADFGIG
                     TSGPWNGLVGRQVIVRVTLQARPA"
     gene            749..1642
                     /locus_tag="VF686_00815"
     CDS             749..1642
                     /locus_tag="VF686_00815"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX8660377.1"
                     /translation="MAVLSAGGLVILPTETVYGLAADAGDARAVASIFEAKGRPRFNP
                     LIAHVADAVQAGEVAVFDRRAQALAEAFWPGPLTIVAPIRDRTGVCDLARAGLDSVAV
                     RVPGHARARALISAFGRPLVAPSANRSGRPSPTTFADAVEETGFAAGAAIDGGPCAVG
                     VESTVVSVLDGHVALLRPGSVTRDEIEAVVGPLDRSGEGHRSPGRLTLHYAPDAPVRI
                     EAEAARDCEILLGFGPGAGDPRWSLSPSGDLREAAANLFRRLREADRERPSGIAVSPI
                     PAHGLGEAINDRLKRAAGFVG"
BASE COUNT          226 a          623 c          624 g          250 t
ORIGIN      
        1 ggcggccgcg aaacggcggg cgaactcatg gacctgcacg agacggcggc caaggggctg
       61 tatgtcctca tcgccctgca cattctcggg gcgctgaagc accagttcat cgaccgggac
      121 aacgtcgtgc accggatgat cctgtggatt ccgcgccgcc cgtgaagctg ctcgccgcct
      181 tgctcgtcgc gctgctggcg cccctgctga tcggcgccgc gccggggccg tggacctttg
      241 agcgggaggc gtcgcgcatc gagatgtccg tccgcgccct tggcgcgacg cgcaccggac
      301 agttcgagga ctggcggggc tcgctggtgc tggatccggc gcgcccggag gaagcgcgag
      361 gctcggtgac ggttcaggca gcctcgctga agatgtcgcc ggcggtcgcg gccagccgcg
      421 ccatcgggcc atcgtttctc gacgccgccc gccatccgac catccgcctc gatctgaagt
      481 cgttggagcc cttgggcgga agccggtata cggcccgcgc ggacatcacg gtgaagggcc
      541 gcaccagggc gatcgccttc ccgctggact tgcgcatcaa cggggagcgg gcgcatctgg
      601 cgggcgcgct gacgctggac cgcgcggatt ttggaatagg aacctcggga ccgtggaacg
      661 ggctggtcgg acggcaggtg atcgtgcggg tgacgcttca ggcgcgcccc gcctgagcgc
      721 atcctccagc gcttccgatg ccgacgccgt ggcggttctg agcgccggcg gtctggtcat
      781 cctgccgacc gagacggtct acggcctggc cgccgacgcc ggcgacgccc gcgccgtcgc
      841 ctcgatcttc gaggccaaag gccgcccccg cttcaaccct ctgatcgccc atgtcgccga
      901 cgcggtgcag gccggagagg tggcggtgtt cgaccgccgc gcgcaggcgc tggccgaagc
      961 cttctggccg ggaccgctga cgatcgtggc ccccattcgc gaccgcaccg gcgtctgcga
     1021 cctcgcccgg gcgggattgg acagcgtcgc agtccgcgtc ccgggccacg cccgcgcccg
     1081 cgccctgata tcggccttcg gacggccgct ggtggcccct tcggccaacc gctcgggccg
     1141 acccagcccc accaccttcg ccgacgccgt ggaagaaacc ggcttcgccg ccggcgccgc
     1201 catcgacggc ggaccatgcg ccgtgggggt cgaaagcacg gtcgtctcgg tactggacgg
     1261 ccatgtcgcc ctgctgcgtc ccggctcggt cacgcgcgac gagatcgagg ccgtcgtcgg
     1321 acctctggac cggagcgggg aggggcatcg ctcgcccggc cggctgaccc tgcactatgc
     1381 gcccgacgct cctgtgcgga tcgaggcgga ggcggcgcgg gactgtgaaa tcctgctggg
     1441 gttcggcccg ggagccggcg atccccgctg gagcctgagc ccgtccggcg atctgcgcga
     1501 agccgccgcc aacctgttcc gccgcctgcg cgaggcggac cgcgaacggc cttcggggat
     1561 cgcggtctcg ccgatcccgg cgcacggact gggtgaggcg atcaacgacc ggctgaagcg
     1621 ggcggcgggc ttcgtgggct agcctcgggg tggacgccgc ccaggctggc gacctcgcgt
     1681 gacaggccgc cgatcgtcac ctcgccaagc cgattcagca ggc
//
LOCUS       DASVVN010000049         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_264865,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000049 DASVVN010000000
VERSION     DASVVN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264865"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..688)
                     /locus_tag="VF686_00820"
     CDS             complement(155..688)
                     /locus_tag="VF686_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660378.1"
                     /translation="MTEAIIRPATAADAEALAVLGRQTFLDTFVDAGGFAIPYPPEDL
                     NPFLEASFSADAVRAKLNEPGAAWWVAERDGELLAFANTGPNTLPHPEAGPGNAELRR
                     LYVSKAAQGLGLGTKLLAVALEWMESHTDGPLWIGVWSGNVKAQKLYQAYGFEKAGEY
                     EYPVGRWRDHEFILRRG"
     gene            753..1673
                     /locus_tag="VF686_00825"
     CDS             753..1673
                     /locus_tag="VF686_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX8660379.1"
                     /translation="MSVALFTHPDMIEHRPGVGHPERPERLKAVLDALDDSGLSLDRR
                     AATEAAVADLERLHPADYVARLLNASPSTGLNQLDADTILSPGSIRAARLSAGAIIDA
                     VRAIARGETARAFAAVRPPGHHAEPDQAMGFCLFSNVAVAARVAQAEGLARVAVIDFD
                     VHHGNGTQAAFENDASLFLGSIHQMPLYPGTGAASETGVGNIVNAPVPPHAAREAWRA
                     TFSGSLMPALQSFNPDLILISAGFDAHRRDPLAHQSLEAEDFAWATRAVLEVARSCCG
                     GKVAASLEGGYDLEGLGRSAVAHVQALGEE"
     gene            1736..2368
                     /locus_tag="VF686_00830"
     CDS             1736..2368
                     /locus_tag="VF686_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8660380.1"
                     /translation="MADPTIPATAQPARPALVSVRPGAIILARHGEPALSRKCLLTSD
                     QYRDWWAKYEVGGLRAGQTPPPELLATAEGAGAIYASTRPRAQETAAAISAGREVMSD
                     ALFIEAPLPPPHFPSWFKLPPKYWGAVARFWWHAFNHHEGQETRRQAECRAEKAAQAL
                     IARAESGQDVLVLAHGYFNHMVGRRLRADGWALVHNQGFKYWSQRRYEKR"
     gene            2385..>2584
                     /locus_tag="VF686_00835"
     CDS             2385..>2584
                     /locus_tag="VF686_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8660381.1"
                     /translation="MIRRTLLALLFCLAAPVAAFAQDPAPAPAVRVAIQTDIGTILAE
                     TDPRAPVTSANFLRYVDEGRFDG"
BASE COUNT          372 a         1007 c          799 g          406 t
ORIGIN      
        1 gcacgaggcc gtcaatcagg gctccagcac ccgcgtcgtc ctgatcttcg acatctggcg
       61 ccccgagctg acggaggaag agcgccatct ggtccagacc ctgctcgagg ccatggacgc
      121 cttctctccc cggaccacct cctgggagta gggatcagcc gcggcgcagg atgaactcgt
      181 ggtcgcgcca gcggccgacc ggatattcgt actcgccggc cttctcgaac ccgtaagcct
      241 gataaagctt ctgcgctttc acgtttccgc tccagacgcc gatccacagc ggcccgtcgg
      301 tgtgggactc catccactcc agcgccacgg ccagcagctt cgtgcccagt cccagcccct
      361 gtgcggcctt ggacacatac agacggcgca actcggcgtt tcccggtccg gcctcgggat
      421 gggggagggt gtttggcccg gtattggcga aggccagcag ttccccgtcc cgctcggcca
      481 cccaccacgc cgcgcccggc tcgttcagct tcgcccgcac ggcgtcggcg ctgaaactgg
      541 cttctaggaa ggggttcagg tcttcggggg gataggggat ggcgaacccg cctgcgtcca
      601 cgaatgtgtc aaggaacgtc tgacgcccca gcacggcgag ggcttcggcg tcggctgcgg
      661 tggcgggtcg gatgatggct tcggtcatgg ttcgcgatta ggaccttgat cggggcacga
      721 caagcccgcg cccggcgcct attctgcctt gaatgagcgt cgccctgttc acccaccccg
      781 acatgatcga acaccgcccc ggcgtcggtc accctgagcg gcccgagcgg ctgaaggccg
      841 tgctggacgc cctcgacgat tccggcctgt ccctcgaccg ccgcgccgcg accgaggccg
      901 ccgtcgccga cctcgagcgc ctgcaccccg ccgactacgt cgcccggctg ctcaacgcct
      961 cgccttccac aggcctgaac cagctcgacg ccgacaccat cctgtcgccg ggcagcatcc
     1021 gcgccgcccg cctgtccgcc ggcgcgatca tcgacgccgt ccgcgccatc gcccgcggcg
     1081 agactgcgcg cgccttcgcc gccgtccgcc cgcccggcca ccatgccgag ccggatcagg
     1141 ccatgggctt ctgcctgttc tccaacgtcg ccgtcgccgc ccgcgtcgcc caggccgagg
     1201 gcctggcccg cgtcgccgtc atcgatttcg atgtccacca cggcaacggc acccaggccg
     1261 ccttcgagaa cgacgccagc ctgttcctcg gctcgatcca ccagatgccc ctctacccgg
     1321 gcacgggcgc ggcctccgag accggcgtcg gcaatatcgt caatgccccg gtcccgcccc
     1381 atgcggctcg cgaggcgtgg cgggcgacct tttccggcag cctgatgccg gcgctgcaaa
     1441 gcttcaatcc ggacctgatc ctgatctccg ccggcttcga cgcccaccgc cgcgatccgc
     1501 tggcgcatca gagtctggag gccgaggatt tcgcctgggc cacccgcgcc gttctcgagg
     1561 tcgcgcgttc gtgttgtggc ggcaaggttg cagcctcgct cgagggcggt tacgacctag
     1621 aagggttggg ccgttccgcg gtcgcccatg tgcaggcctt gggggaggaa taaaggacag
     1681 ttcgccaacg gcttattgtc cacgcatcat gcccgccccc gcccgagact cattgatggc
     1741 agacccgacg attcccgcga ctgcccagcc ggcccgcccg gctctggtct cggtgcgtcc
     1801 cggcgccatc atcctggccc gccacggcga gccggcgctg tcgcgcaaat gcctgctgac
     1861 ctccgaccag taccgcgatt ggtgggcgaa atacgaagtc ggcggcctgc gcgccggcca
     1921 gaccccgccg cccgaactat tggccacggc cgagggcgcg ggcgccatct acgcctcgac
     1981 ccgtccccgc gcgcaggaaa ccgccgccgc catttcggcc ggccgcgagg tcatgtcgga
     2041 cgccctgttc atcgaggcgc ccctgccgcc gccgcacttc ccctcatggt tcaagctgcc
     2101 gcccaaatac tggggcgccg tggcccgctt ctggtggcac gccttcaacc accacgaagg
     2161 tcaggaaacc cgccgtcagg ccgaatgccg cgccgaaaag gccgcccagg ccctgatcgc
     2221 ccgcgccgag agcggtcagg acgtgcttgt cctcgcccac ggctatttca accacatggt
     2281 cggccgccgc ctgcgcgccg acggctgggc cctcgtccac aaccagggct tcaaatactg
     2341 gtcccagcgc cgctacgaga agcgctgacg gcgcggagag cccgatgatc cgccgcaccc
     2401 tgctcgccct gctgttctgc ctcgccgccc cggtcgccgc cttcgcgcag gatccggctc
     2461 ccgcccccgc cgtgcgcgtg gccatccaga ccgacatcgg gaccatcctc gccgagaccg
     2521 acccgcgcgc gccggtcacc tcggccaact tcctgcgcta cgtcgacgag ggccggttcg
     2581 acgg
//
LOCUS       DASVVN010000050         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_238773,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000050 DASVVN010000000
VERSION     DASVVN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238773"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF686_00840"
     CDS             complement(<1..208)
                     /locus_tag="VF686_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX8660382.1"
                     /translation="MPHPSPLISTEDLAARLGDPALRLVDASWHLDGRDGRPDFEAAR
                     LPGAVFFDLDASSDPDSPLPHMLPT"
     gene            complement(238..1458)
                     /locus_tag="VF686_00845"
     CDS             complement(238..1458)
                     /locus_tag="VF686_00845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8660383.1"
                     /translation="MTSVTAERHEAGARTPRGFSLLLRLLASNWTFGRLTVLLPNGEV
                     HQLEGSQPGVSAIFDIRDYRFARRVLASGDIGFAEGYMAGEWESPHLAALLESFANNY
                     DHIRRLVDGHPAMKAVHWFTHRMNRNSRKGSRKNIHAHYDLGNAFYASWLDSTMTYSS
                     ARFTRAGMALDGAQREKYASLARLMDLQSGQSVLEIGCGWGGFAEFAAKEAGARVTAV
                     TISQEQYDFARQRMFNAGLSDHVDIQLIDYRDVEGTFDRVASIEMFEAVGIEYWPAYF
                     GKIHDVLKPGGKAGLQIITIDDALFGGYNKRTDFIQKYIFPGGMLPSENALKPVIDRA
                     SLEWRAIERFGQDYAETLKLWDERFQAAWGDIRRMGGFDERFRRLWRFYLAYCEAGFR
                     SARTDVVQLVLARG"
BASE COUNT          331 a          605 c          657 g          317 t
ORIGIN      
        1 gcgtcggcag catgtgtggc agcgggctgt ccgggtccga actggcgtcc agatcgaaga
       61 agacggcgcc gggcaggcgc gccgcctcga aatccggtcg tccgtcgcgc ccgtccagat
      121 gccagctggc gtcgacgagg cgcagggcgg gatcgccgag gcgggcggcc aggtcttcgg
      181 tcgagatcag gggcgaggga tggggcatgg tggtgctcgg gcggcgtccg gcgcgcgtca
      241 gccgcgcgcg agaaccagct gtaccacgtc ggttcgggcc gagcggaagc cggcctcgca
      301 ataggccagg tagaagcgcc acaggcgccg gaaccgctcg tcgaacccgc ccatgcggcg
      361 aatgtcgccc caggccgcct ggaagcgctc atcccagagc ttcagggtct cggcatagtc
      421 ctgaccgaac ctttcgatgg cgcgccattc caggctggcg cggtcgatga cgggtttcaa
      481 cgcgttctct gacgggagca tgccgccggg gaagatgtat ttctggatga agtccgtgcg
      541 cttgttgtag ccgccgaaca gggcgtcatc gatggtgatg atctgaagac ccgccttgcc
      601 gccgggcttc agcacgtcgt gaatcttgcc gaaataggcc ggccagtatt cgatgccgac
      661 ggcctcgaac atctcgatcg aggcgacgcg gtcgaaggtc ccctcgacgt cgcgatagtc
      721 gatcagctgg atgtcgacgt gatccgacag gccagcgttg aacatgcgct gacgggcgaa
      781 gtcgtactgc tcctgcgaga tggtgacggc cgtgacgcgt gcgccggcct ccttggccgc
      841 gaattcggcg aagccgcccc agccacagcc gatctccagc accgactgac ccgattgcag
      901 atccatcagc cgggcgagag aggcgtattt ctcgcgctgg gccccgtcca gcgccattcc
      961 cgcccgtgtg aaccgggccg aggaataggt catcgtggag tccagccacg aggcatagaa
     1021 ggcgttgccg agatcgtagt gggcgtggat gttcttgcgc gagcccttgc ggctgttgcg
     1081 gttcatccgg tgcgtgaacc agtggaccgc cttcatggcc ggatgtccgt ccaccaggcg
     1141 gcgaatgtga tcgtagttgt tggcgaagga ttccagcagg gctgcaaggt gcgggctttc
     1201 ccactcgccg gccatatagc cctcggcgaa gccgatgtcc ccggaggcca gaacgcgacg
     1261 ggcgaaacgg tagtccctga tgtcgaaaat cgccgacacg cccggctggg agccttccag
     1321 ctgatgcacc tcgccgttgg gcagcaggac ggtcaggcgg ccgaaggtcc agttggaggc
     1381 cagaaggcgc aacagcaggg agaacccgcg gggggtgcgg gcgcctgcct catgtcgctc
     1441 ggcggtgacc gacgtcatgg gcgcgactct ccaatggagg acggacgggc gtcaatccct
     1501 ctgagccctg atcgggaggc ggagcgccgg accctgttgg acgtcacaac cgtctgcatc
     1561 gatgtctgcc cctgtatgac cggatgagtc cggaactacg gtgaagcatg ggctgcggat
     1621 ccaccgatcc gctgctttct gcacattgga acaggacccg gcgtcggcaa gggcatcttc
     1681 gccgttggtg gtcatcgggc cgggcgcctg ataacttgcg gacctcgccg tcagacgcca
     1741 cctgatcgcc gcatgagtgc ggcatcaacc gcgcgtcgcc agccgcaaat ccgggaagcc
     1801 cgccttgtcc aagcccaacg ccgtcatcga catcaaggaa ggcctcaagc ggcccgtcag
     1861 catcggcggc ggtcagcgca tcgtcttcat cgccggcccc tgccagctgg
//
LOCUS       DASVVN010000051         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_1969, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000051 DASVVN010000000
VERSION     DASVVN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VF686_00850"
     CDS             <1..723
                     /locus_tag="VF686_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660384.1"
                     /translation="FGARWPDRVCPGVVNLDNTAAQAVNDRIAELAGTLDIGVGQPGC
                     DPNVVVIFTADGRSLAAEMVQRDRRLFSHPQLGTFNRGDAALLDFASSDAPVRWWHLS
                     MPVDVVTRERAVRLSEDLRPVSATGAEGRLSNSVVDVLYKAIIIVDVTRLGDTAFEQV
                     ADYIAMVAMAQIDPEAEIDGHHTILNVFRQPDVSPGLTEWDQSYLEALYSSASMRRNP
                     GSRSSDIARLMTRDQRAEASDE"
     gene            complement(813..2156)
                     /gene="tig"
                     /locus_tag="VF686_00855"
     CDS             complement(813..2156)
                     /gene="tig"
                     /locus_tag="VF686_00855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX8660385.1"
                     /translation="MQVVEKSNEGLSRVIAVTIPVAELNQKLDAKLKEVAPQMKLKGF
                     RPGKVPMSHVKKTFGRDLMGEIVNDTLNSSSQKALDDAKVRPAAPAEMKLTSDMDKVI
                     AGQEDLAYEMALEVMPDFTPTDIKKLKLERPTYEASDADLDEALTELAGQAKSYVEKT
                     GKTVKAAEGDEVTIDFLGKLDGEPFEGGAAEDTQLVIGSNRFIPGFEEQLKGAKVGEE
                     KVLNVTFPEDYQAAHLAGKAVTFDVKVKAIKAEAPAVVDEDFAKRIGIESLDKLKELL
                     RTNLNQQYTGAARFKLKRALLDALDKAHDFALPPKMVEAEFDGIWQQVQADKEAGRLP
                     EEDAKKSEKKLKEEYRKIAERRVRLGLVLAEIGRANNVQVTDQELNNAIMNEARNYPG
                     QERQVLDFYRQNPNAAAQMRAPIYEEKVCDLIFDTAKVTDTPITKEELLKEDDDI"
     gene            2352..3053
                     /locus_tag="VF686_00860"
     CDS             2352..3053
                     /locus_tag="VF686_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8660386.1"
                     /translation="MSSSPPPIGVAVFPVTPLQQNCTLVWCRKTLKAAIIDPGASVDA
                     LLAAVVKQGLTLDAIWITHGHLDHASGAAEMQEKTGLDIIGPHPDDQFWIDGIPAQAE
                     LYGLTGARSFTPTRWLADGDTLTLGDTAWEVYHCPGHTPGHVIFFNRAARFAQVGDVL
                     FQGSIGRTDFPRSDHASLLNAIVTKLWPLGDDVAFVPGHGPTSTFGVERRTNPFVSDE
                     AMRRAPIARPASGPS"
BASE COUNT          470 a         1024 c         1016 g          604 t
ORIGIN      
        1 ttcggggccc gatggccgga ccgcgtgtgt cccggcgtag tcaatctcga caacaccgcc
       61 gcgcaggccg tcaatgaccg gatcgcggag ctcgcgggga ctctggacat cggcgttggc
      121 caaccgggat gcgatcccaa tgttgtcgtc atcttcacgg cggatgggcg gagtctcgcc
      181 gcagagatgg ttcagcgtga ccgtcgcctg ttctcccacc cgcagttggg cacgttcaac
      241 aggggagacg cggcgctgct ggatttcgcg agctccgacg ctccggtccg ctggtggcat
      301 ctcagcatgc cggtcgacgt tgttacccgg gagcgcgcgg ttcgcctttc ggaggatctc
      361 cggcctgtgt cggcgaccgg cgccgaggga cggctcagca attcggtcgt cgatgtcctg
      421 tacaaggcga tcatcatcgt cgacgtgaca cggctgggcg acacggcctt tgaacaggtt
      481 gcggactaca tcgccatggt cgccatggcc cagatcgatc cggaggcaga gatcgacggg
      541 caccacacga tcctcaacgt cttccgccag ccggacgttt cgcccggcct gaccgaatgg
      601 gaccagtcct atctggaagc cctgtattcg tcggcgtcca tgcgcaggaa tccgggcagc
      661 cgcagttcgg acatcgcccg cctgatgacg cgcgatcaac gggcggaggc atcggacgag
      721 tagccgggcc aggacgtccc gccggcacaa aacgaaacgg gccgccgctg cgattgcaac
      781 ggcggcccga aaaatcgtcg atcggaagcc gatcagatgt cgtcgtcttc cttgagcagc
      841 tcttccttgg tgatcggcgt gtcggtcacc ttggccgtgt cgaagatcag gtcgcagacc
      901 ttctcctcat agatcggcgc gcgcatctgg gcggcggcgt tggggttctg gcggtagaag
      961 tccagcacct gacgctcctg gcccggatag ttgcgggcct cgttcatgat cgcgttgttc
     1021 agctcctgat cggtgacctg aacattgttg gcgcggccga tctcggccag caccagaccc
     1081 aggcgcacgc ggcgctcggc gatcttgcgg tattcttcct tgagcttctt ctcggacttc
     1141 ttggcgtcct cttccggcag gcggccggct tccttgtcgg cctggacctg ctgccagatg
     1201 ccgtcgaact cggcctcgac catcttgggc ggcagggcga agtcgtgggc cttgtccaga
     1261 gcgtccagca gggcgcgctt cagcttgaag cgggcggcgc cggtgtactg ctggttcagg
     1321 ttggtgcgca gcagctcctt gagcttgtcc agcgactcga tgccgatgcg cttggcgaag
     1381 tcctcgtcga ccacggcggg ggcctcggcc ttgatggcct tgaccttgac gtcgaaggtc
     1441 acggccttgc cggccagatg ggcggcctgg tagtcctcgg ggaaggtcac gttcaggacc
     1501 ttctcttcgc cgaccttggc gcccttcagc tgctcttcga agccggggat gaagcggttc
     1561 gagccgatga ccaactgggt gtcctcggcg gcgccgccct cgaagggttc gccatccagc
     1621 ttgcccagga agtcgatggt gacctcgtcg ccctcggcgg ccttcacggt cttgccggtt
     1681 ttctcgacat acgatttcgc ctggccggcc agttcggtca gggcctcgtc cagatcggcg
     1741 tctgacgctt cataggtcgg gcgttccagc ttcagcttct tgatgtcggt cggggtgaag
     1801 tcgggcatga cttccaaggc catttcatag gccaggtctt cctgaccggc gatgaccttg
     1861 tccatgtccg aggtcagctt catctcggcg ggggcggcgg gacgcacctt ggcgtcgtcc
     1921 agggccttct ggctcgagct gttcagtgtg tcgttgacga tttcgcccat cagatcgcga
     1981 ccgaaggtct tcttgacgtg cgacatcggg accttgccgg gacggaagcc cttcagcttc
     2041 atctgcggcg cgacttcctt cagcttggcg tccagcttct ggttcagctc ggcgacggga
     2101 atggtcaccg cgatgacgcg gctgagacct tcgttggact tttcgacaac ttgcatggcc
     2161 gggattctga cgctctgtgg cggcgcctta tcaggcaggc gctgcgcggt gtgggtaaag
     2221 caacaagggc cgccctttgg aggcggcgcc tttgaagccc gcccttatgt cgacgacggt
     2281 cccaaaggtc aacgtgagac gcggcgcagg ggacggtttc ggtcggagcc gcagcgcgct
     2341 atctggacgc catgagctcc tcccctcccc cgatcggcgt cgctgtcttc ccggtcacgc
     2401 ccctgcagca gaactgcacc ctcgtctggt gccgcaagac gctgaaggcc gcgatcatcg
     2461 atccgggcgc ttcggtggac gccctgctgg ccgccgtcgt caagcagggg ctgacccttg
     2521 acgccatctg gatcacccac ggccacctcg atcacgccag cggcgcggcc gagatgcagg
     2581 agaagaccgg gctcgacatc atcggtccgc atccggacga ccagttctgg atcgacggca
     2641 ttccggccca ggccgaactg tatggcctga cgggcgcccg cagcttcacc ccgacccgct
     2701 ggctggccga cggggacacg ctgacgctgg gggacacggc ctgggaggtc taccactgcc
     2761 ccggccatac gcccggccat gtgatcttct tcaaccgtgc ggcccgcttc gcccaggtgg
     2821 gggacgtgtt gttccagggc tcgatcggac gcaccgactt cccgcgcagc gaccacgcca
     2881 gcctgctgaa cgcgatcgtt acaaagctct ggccgctggg cgacgacgtc gccttcgtgc
     2941 cggggcacgg cccgacctcc accttcgggg tcgagcgccg caccaatccg ttcgtctcgg
     3001 acgaggcgat gcgccgggcg cccatcgcgc gcccggcgtc cggcccgtcc tgatgtcgct
     3061 gacctagcgc gcggccagca ggccgatcac gacaccaatg ccgagggcgt acag
//
LOCUS       DASVVN010000052         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_27971, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000052 DASVVN010000000
VERSION     DASVVN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27971"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VF686_00865"
     CDS             <1..807
                     /locus_tag="VF686_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660387.1"
                     /translation="GDVVRLKQILTNLVSNAVKFTGTGFVSLTAAPGPDCDGAPTLRF
                     SIEDTGIGFDCETRDRLFSRFEQADGAITRKFGGSGLGLAISRQLAEMMGGDLDCESE
                     PGGGSAFILTIPFRPVEQPAAAGLSAVATEDGDRPVIRVLLADDHPVNRKVVEMILAQ
                     ASVEIVSVEDGAQAVQACRDADYDLILMDMQMPVMDGLTATREIRLHEAAMGLTRTPI
                     VMLTANALPEHIAMADAAGADRHLAKPFDAAELLDLVVTLPRAAAEAIAA"
     gene            complement(811..1020)
                     /locus_tag="VF686_00870"
     CDS             complement(811..1020)
                     /locus_tag="VF686_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660388.1"
                     /translation="MQPLNNPTAISDFCLAPLNLTDGTEAEREVRRRLEHVIKTFRAQ
                     LAQPVSVDFSSMPSQVINEAAHGYE"
     gene            complement(1035..>2215)
                     /gene="aceA"
                     /locus_tag="VF686_00875"
     CDS             complement(1035..>2215)
                     /gene="aceA"
                     /locus_tag="VF686_00875"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX8660389.1"
                     /translation="PVTHTLATRGANRLWELLHEEPFINALGAVTGNQAMQMVRAGLK
                     AIYLSGWQVAADANTAGAMYPDQSLYPANAAPELCRRINRTLQRADQIEHAEGGAKRD
                     WFAPIVADAEAGFGGPLNSFEIMKAFIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTQ
                     AHERNLIAARLAADVMGTPTLTVARTDAESAQLITSDVDERDRPFIDRDSRTPEGFFR
                     LKPGTGLDHCIARGLSYAKYADVLWWETSHPDLDDARRFAEAVHREHPGKLMAYNCSP
                     SFNWKAKLDDATIARFQRELGAMGYKFQFVTLAGFHSLNNAMFELADGYRDRGMAAYS
                     ELQQREFANEAGGYTATRHQREVGTGYFDQVATVISNGRSSTTALKDSTETAQFVAAE
                     "
BASE COUNT          327 a          765 c          745 g          378 t
ORIGIN      
        1 ggcgacgtgg tccggctcaa gcagatcctg accaatctgg tctccaacgc cgtgaaattc
       61 accggcacgg gcttcgtcag cctgaccgcc gcccccggtc ccgactgtga cggcgcaccg
      121 acgctgcggt tctcgatcga agacaccggc atcggcttcg actgcgagac gcgcgaccgc
      181 ctgttcagcc gcttcgaaca ggccgacggc gcgatcaccc gcaaattcgg cggctccggg
      241 ctcggcctcg ccatttcgcg ccagctggcc gaaatgatgg gcggcgatct ggattgcgaa
      301 agcgagcccg gcggcggctc ggccttcatc ctgaccatcc cgttccgccc cgtcgagcaa
      361 ccggccgccg cgggtctgtc ggccgtcgct acggaggacg gcgaccggcc tgtgatccgc
      421 gtcctgctgg ccgacgacca cccggtgaac cgcaaggtgg tcgaaatgat cctggcccag
      481 gcctcggtcg agatcgtatc ggtcgaggac ggcgcccagg ccgtgcaggc ctgccgcgac
      541 gccgactacg acctgatcct gatggacatg cagatgccgg tcatggacgg cctgaccgcc
      601 acgcgcgaga tccggctgca cgaggcggcc atgggcctga cccgcacccc gatcgtcatg
      661 ctgaccgcca acgccctgcc cgagcacatc gccatggccg atgcggccgg cgccgaccgg
      721 catctggcca agccgttcga tgcggccgaa ctgctggatc tggtcgtcac cctgccgcgc
      781 gccgcggccg aggcgatcgc cgcctgaacc ctattcgtag ccgtgcgcgg cttcgttgat
      841 gacctgcgaa ggcatggacg agaagtcgac gctgacaggc tgggccagct gggccctgaa
      901 cgtcttgatc acatgctcca gccgcctgcg cacctcgcgt tcggcctccg tgccgtcggt
      961 caggttcagc ggggccaggc agaagtcgct gatggccgtg ggattgttca gcggttgcat
     1021 ggggtctctc ctgattattc ggccgccacg aactgggcgg tctcggtgga atccttcagc
     1081 gcggtcgtgg acgaccggcc gttcgagatg acggtggcga cctgatcgaa atagccggtg
     1141 ccgacctcgc gctggtgccg ggtcgcggtg tagccgcccg cctcgttggc gaactcgcgc
     1201 tgctgcagct cggaataggc ggccatgccg cggtcgcggt agccgtccgc cagctcgaac
     1261 atcgcgttat tcaggctgtg gaagcccgcc agagtcacga actggaattt gtagcccatg
     1321 gcccccagct cgcgctggaa tctggcgatg gtggcgtcgt ccagtttggc cttccagttg
     1381 aacgacggcg aacagttgta ggccatcagc ttgcccgggt gctcgcggtg cacggcctct
     1441 gcgaagcggc gggcgtcgtc cagatcgggg tgcgacgtct cccaccacag gacatcggcg
     1501 tatttcgcgt acgacaggcc gcgcgcgatg caatggtcca gccccgtgcc cggcttcagc
     1561 cggaagaagc cctcgggcgt gcggctgtcg cggtcgatga agggccggtc gcgttcgtcc
     1621 acgtccgagg tgatcagctg ggcgctctcg gcgtccgtgc gggccaccgt cagggtcggc
     1681 gtgcccatga cgtcggcggc cagacgggcg gcgatcaggt tgcgctcgtg cgcctgggtc
     1741 gggatcagca ccttgccgcc cagatggccg cacttcttct cgctggccag ctggtcctcg
     1801 aaatggactc ccgccgcccc ggcctcgatg aaggccttca tgatctcgaa gctgttcagc
     1861 ggcccgccga acccggcctc cgcgtcggcg acgatcgggg cgaaccagtc gcgctttgca
     1921 ccaccttccg cgtgttcgat ctggtcggcg cgctgcaggg tgcggttgat gcggcggcac
     1981 agttccggcg cggcgttcgc cggatacagc gactggtccg gatacatggc cccggccgta
     2041 ttggcgtccg ccgccacctg ccagcccgac agatagatgg ccttcagccc cgcacggacc
     2101 atctgcatgg cctgattgcc ggtcaccgcg ccgagggcgt tgatgaacgg ctcctcgtgc
     2161 aagagctccc acaggcggtt ggccccccgg gtcgccagcg tatgggtcac gggca
//
LOCUS       DASVVN010000053         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_343925,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000053 DASVVN010000000
VERSION     DASVVN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343925"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /gene="lipB"
                     /locus_tag="VF686_00880"
     CDS             complement(<1..715)
                     /gene="lipB"
                     /locus_tag="VF686_00880"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517631.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8660390.1"
                     /translation="MLSQALSSAASKLVRADGRPVEWAVSDGLVGYEAAVAAMEARAA
                     AIAAGEAAELVWLLEHPPLYTAGVSSKDGDLLDAGRFPVHRTGRGGQFTYHGPGQRVA
                     YVMLDLNARGKDVRAFVRGLEQWLIGALGEFGVPADVREGRVGVWVERKGPGWSREDK
                     IAAIGVKVRKWVSFHGVSLNVEPDLSHFGGIVPCGIAEHGVTSLVDLGVLATMDEADA
                     ALKTSFGRVFGAVVAGEAPV"
     gene            746..1042
                     /locus_tag="VF686_00885"
     CDS             746..1042
                     /locus_tag="VF686_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX8660391.1"
                     /translation="MQVQTAPLVSVAESPMSQIEAVEVEISVVLGRSVLPMSQLLRMG
                     RGAVIPLDAHESDEVWILANNHPVARGEIEIREDRIAITVTRAADVYDFMAGAA"
     gene            1093..1179
                     /locus_tag="VF686_00890"
     tRNA            1093..1179
                     /locus_tag="VF686_00890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Leu,seq:gag)
     gene            1241..>1896
                     /locus_tag="VF686_00895"
     CDS             1241..>1896
                     /locus_tag="VF686_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8660392.1"
                     /translation="MITTATAPLPPSLDDDIDHDALDEDYVLTASFVEKVVDASDAGD
                     GLKLRSLLEDLHPADVADLMGFLTAEHRAVVVLWLPADLLAETLPELDDNIREEVLER
                     VPHLTLAEALQELDSDDAAAVVEDLEDDPREKVLAAMPEVDRAAIESSLRYAEDSAGR
                     LMQREVMAAPSFWNVGDTIDHIRKQGEDLPELFFDIYIVDPSNRPVGSVPVSGLLRAA
                     R"
BASE COUNT          323 a          692 c          595 g          286 t
ORIGIN      
        1 gcaccggcgc ttccccagcg accaccgcgc cgaacacccg cccgaagctc gtcttcagcg
       61 ccgcatccgc ctcgtccatc gtggccagaa cgccaaggtc gaccagacta gtgactccgt
      121 gctcggcgat gccgcacgga acgatgccgc cgaaatggct gaggtccggc tccacgttca
      181 ggctgacgcc gtggaagctg acccatttgc ggaccttcac cccgatggcg gcgatcttgt
      241 cctcgcgcga ccagccgggg cccttgcgct cgacccagac cccgacccgc ccctcgcgca
      301 cgtcggcggg cacgccgaac tcgcccagcg cgccgatgag ccactgctcc agtccccgca
      361 cgaaggcccg cacgtccttg ccgcgcgcat tcaggtccag catcacatag gccacccgct
      421 ggcccggccc gtggtaggtg aactgcccgc cccgcccggt ccggtggacg gggaaccgcc
      481 ctgcatccag aaggtcgccg tccttcgacg acacccccgc cgtgtagagc ggcggatgct
      541 ccagcagcca gaccagctcg gccgcctcgc ccgccgcgat ggccgccgcc cgggcctcca
      601 tggcggccac ggccgcctcg tagccgacaa ggccgtcgga caccgcccat tcaaccggac
      661 ggccgtcggc gcggaccagt ttcgatgcgg ctgagcttaa ggcttgggaa agcatgacgc
      721 ccccaatgtg ggggctggga gcgccatgca agtccagacc gcgccattag tatccgtagc
      781 cgagtccccc atgagccaga tcgaagccgt cgaagtcgag atctcggtcg tccttgggcg
      841 ctccgtgctg cccatgagcc agctgctgcg gatgggccgc ggggccgtga tcccgctcga
      901 cgcccatgag tcggacgagg tctggatcct cgccaacaac cacccggtgg cccgcggcga
      961 gatcgagatc cgcgaagacc gcatcgccat caccgtgacc cgggcggccg acgtctacga
     1021 tttcatggcc ggagccgcct gaaccgcttg gcagcggccg atctctccgc tatcagcccc
     1081 cctccgatgc gagcggtcgt ggcggaattg gtagacgcgc agcgttgagg tcgctgttcc
     1141 ttaaccggag tggaagttcg agtcttctcg accgcaccat cgaaacaaca gggcgaatct
     1201 cggctacgat tgagctgaca taaaagcagg gaggccaagc gtgatcacca ccgcaacagc
     1261 ccccctgccg ccttctctcg acgacgacat agatcacgac gccctggacg aggactatgt
     1321 cctgacggcc tcgttcgtcg aaaaggtcgt cgatgcttcg gacgccggcg atggcctgaa
     1381 gctccgctcc ctgctggaag accttcaccc ggccgacgtg gccgatctga tgggctttct
     1441 gacggccgag caccgcgccg tcgtggtgct gtggctgccg gccgacctgc tggccgaaac
     1501 cctgcccgaa ctggacgaca acatccgcga ggaggtgctg gagcgcgtcc cgcacctgac
     1561 cctggccgag gccctgcagg agctggactc cgacgacgcc gccgccgtgg tcgaggatct
     1621 ggaggacgac ccgcgcgaaa aagtgttggc ggccatgccc gaggtcgacc gcgccgcgat
     1681 cgaaagctcg ctgcgctacg ccgaggactc cgccggccgt ctgatgcagc gcgaggtcat
     1741 ggccgcgccg tcgttctgga acgtcggcga caccatcgac cacatccgca aacagggcga
     1801 agacctgccg gagctgttct tcgacatcta tatcgtcgat ccgtcgaacc ggccggtcgg
     1861 cagcgtgccg gtcagcggtc tgttgcgcgc ggcacg
//
LOCUS       DASVVN010000054         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_2090, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000054 DASVVN010000000
VERSION     DASVVN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF686_00900"
     CDS             <1..297
                     /locus_tag="VF686_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660393.1"
                     /translation="ATQDYRVNGQGFRIYGGAGVMAIAGDRQATGDMRFTGAPIGFGT
                     FTVEGAEAPPMAGLVDFGVEVGAGEGVTVSAGYRGLFSERLEDNQIGIKLNVSW"
     gene            390..1358
                     /locus_tag="VF686_00905"
     CDS             390..1358
                     /locus_tag="VF686_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8660394.1"
                     /translation="MAGAFAGRDLMSDRRPLLAGVELGGTKCVCLLAAGPDDVREEVR
                     IETTTPGETLAAIQTVLTRWRGAEGFAAIGVASFGPLELDPGAVGYGRLVNTSKSGWS
                     GADVLAPVRGFGVPIGFDTDVNGAALAEGRWGGAQGLESHAYVTVGTGIGVGSVVRGR
                     TVRGLGHSEAGHLRIPRLAGETWPGVCPYHGDCVEGLASGPAIQAKTGQPGDRLTPSD
                     PAWDEVVHALVALLHNLALTTAPQRILIGGGVVGGRPFLLPRLRTALIHSLNGYGAAP
                     EIVARIEDFLAAPALTDRAGPLGAIALAEDALGLMEPAAIRPASRL"
     gene            complement(1333..1485)
                     /locus_tag="VF686_00910"
     CDS             complement(1333..1485)
                     /locus_tag="VF686_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660395.1"
                     /translation="MRAVREFWPVLMGAAILSVASAWVVIKLDRDGAAVQPERYERFI
                     NGTRGV"
     gene            1628..>1830
                     /locus_tag="VF686_00915"
     CDS             1628..>1830
                     /locus_tag="VF686_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX8660396.1"
                     /translation="MPISFAQFAPILMLTASNIFMTLAWYGHLKFGSKPLWLVVIVSW
                     GIAFFEYCLAVPANRIGHQVYSAA"
BASE COUNT          266 a          639 c          625 g          300 t
ORIGIN      
        1 gcgacgcagg actatcgcgt caacggccaa ggcttccgca tctacggcgg ggccggcgtc
       61 atggctatcg cgggcgaccg tcaggccacc ggcgacatgc gcttcaccgg cgcgccgatc
      121 ggcttcggga ccttcaccgt cgagggcgcc gaagccccgc cgatggccgg tctcgtcgac
      181 ttcggcgtgg aggtcggcgc cggcgagggc gtcaccgtct cggccggcta tcgcggcctg
      241 ttcagcgaac gtctcgagga caaccagatc ggcatcaagc tgaacgtcag ctggtaacat
      301 cgcagcggcg tctccgtccg aaagggcggg ggcgtcagct gttccccaaa ccttgtgtgc
      361 ggttccgcta cgggcgatgg attatgtcca tggcgggtgc gttcgcgggg agggatctga
      421 tgtcggaccg gaggccgctg ctcgcggggg tcgaactggg cgggaccaaa tgcgtctgcc
      481 tgctggccgc cggcccggac gacgtccgcg aggaagtgcg gatcgagacg accactccgg
      541 gtgaaaccct cgccgccatc cagacggtcc tgacgcgctg gcgtggcgcc gaaggcttcg
      601 ccgccatagg cgtggccagt ttcggtccgc tcgagctcga tcccggggcc gtcggatacg
      661 gccgcctcgt caacacctcc aagtccggct ggagcggcgc cgacgtgttg gcgccagtgc
      721 gcggtttcgg cgtcccgatc gggttcgaca cggacgtcaa cggcgccgct ctggccgagg
      781 gacggtgggg cggggcgcag ggcctcgaaa gccatgccta tgtcaccgtc ggcacgggca
      841 tcggcgtcgg atccgttgtg cggggccgca cggtccgggg cctgggccat tccgaggccg
      901 ggcacctgcg catcccgcgg ctggccggtg agacctggcc cggcgtctgc ccctatcatg
      961 gagactgcgt cgagggtctg gcctccggtc cggcgatcca ggcgaagacg ggccagcccg
     1021 gcgatcggct gacgccgtcc gacccagcct gggacgaggt ggtccacgcc ctcgtcgccc
     1081 tgctgcacaa tctggccctg accacggccc cgcagaggat tctgatcggc ggcggggtgg
     1141 tgggcggacg acccttcctg ctgccgcggc tgagaacggc gctgatccac agtctgaacg
     1201 gctacggagc ggcgcccgaa atcgtcgccc ggatcgagga cttcctcgcg gcccccgctc
     1261 ttacggaccg cgccggcccg ctgggcgcca tcgctttggc cgaagacgcg ctgggcttga
     1321 tggagccggc tgctatacgc cccgcgtccc gtttatgaag cgctcgtacc gttcgggctg
     1381 aacagccgcc ccgtcgcgat ccagcttgat cacgacccac gcgctcgcga ccgacaggat
     1441 cgccgcgccc atcagcaccg gccagaattc acgaacagcc cgcacggcgg cgtctccaat
     1501 cggcgcgttc aaacgcgcga cccgcgccgg aaacgggcgc agcccgtagc ggttcctgtg
     1561 tccgttcgcg caccttgacg gctagcgtcc cggctggtgc atgaaggcac gccgccaagg
     1621 gttccgcatg ccgatctcat tcgcccaatt cgcccccatc ctgatgctga cggcgtccaa
     1681 catcttcatg accctcgcct ggtacgggca tttgaagttc ggctcgaaac ccctgtggct
     1741 ggtcgtcatc gtcagctggg ggatcgcctt cttcgaatat tgcctcgccg tgcccgccaa
     1801 ccggatcggg catcaggtct attcggccgc
//
LOCUS       DASVVN010000055         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_28099, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000055 DASVVN010000000
VERSION     DASVVN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28099"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VF686_00920"
     CDS             <1..1258
                     /locus_tag="VF686_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HEX8660397.1"
                     /translation="IGQAVAKAGADAAVLTAPASLAWLFNIRGGDVIRTPLPLGQAVV
                     KADGTAKLFLDPRKVTNELPGWLGDEVTIEAPEALERALDALSGQKVLVDPGQSSAWY
                     FDRLTAAGATVVRGMDPCTLPRACKNPVEIEGARQAHIRDGAALTRFLHWVDTVGQET
                     LPDERQVAEALEGFREATGGLKDLSFDTIAGAGPNGALPHYKPVARTIRRMEKGSLLL
                     VDGGGQYLDGTTDVTRTMAVGEPTADQRRMFTLVLKGHIAMAVVRFPAGTTGHQLDAM
                     ARLPLWMQGFDYDHGTGHGVGSYLGVHEGPQRIAKAVNSQPLLTGMILSNEPGYYREG
                     HWGIRIETLQVVTPPTPIPGGERPMHGFEQLTLAPLDRRLIDVPLLTPDERAYVDAYH
                     AEVLAKVGPLLDGEVRAWLEASCAAL"
     gene            1255..1674
                     /locus_tag="VF686_00925"
     CDS             1255..1674
                     /locus_tag="VF686_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660398.1"
                     /translation="MNRLGLIAVAAGLTVAGCDPAEPRTQPVEPTPEAMAAAVNPADI
                     WCLEPETLRVEPIASDAVWARWRHWAICDYDARKAAHYLDILVARDDPDALRAKGESL
                     RNSDPVESDRLLRRAERFGWREPTFRDEINKLATPPA"
BASE COUNT          265 a          550 c          622 g          257 t
ORIGIN      
        1 catcggtcag gcggtggcga aggccggcgc cgacgcggcg gtgctgacgg cgccggcttc
       61 gctggcctgg ctgttcaaca tccgcggcgg cgacgtgatc cgtacgcccc tgccgctggg
      121 acaggcggtg gtgaaggcgg acgggacggc gaaactgttt ctcgatcccc ggaaggtgac
      181 caatgaactg cccggctggc tgggcgacga agtgacgatc gaggcgcccg aggcgctgga
      241 gcgcgcgctg gacgccctgt cggggcagaa ggtgctggtc gatccgggcc agtcctcggc
      301 ctggtatttc gaccgactga ccgccgccgg ggccacggtg gtgcgcggga tggatccctg
      361 caccctgccg cgggcctgca agaatccggt cgagatcgag ggcgcgcgtc aggcccacat
      421 ccgcgacggc gcggccctga cccgcttcct gcactgggtc gacaccgtcg ggcaggaaac
      481 cctgcccgac gagcgtcagg tggccgaggc gctggaagga ttccgcgagg cgacgggggg
      541 gctgaaggac ctgtcgttcg acaccatcgc cggcgccggg ccgaacggcg ccctgccgca
      601 ctacaagccg gtggcgcgca ccatccgccg gatggagaag ggttcgctgc tgctggtcga
      661 cggcgggggt cagtatctgg acggcaccac cgatgtgacc cgcaccatgg cggtgggcga
      721 gccgacggcc gatcagcgcc gcatgttcac ccttgtgctc aagggccata tcgccatggc
      781 cgtggtgcgg ttcccggccg gcacgaccgg acatcagctg gacgcgatgg cgcgtctgcc
      841 gttgtggatg cagggcttcg actacgacca cggcaccggc cacggcgtcg gctcgtatct
      901 gggcgtgcac gaggggccgc agcggatcgc caaggcggtc aacagccagc cgctgctgac
      961 cggcatgatc ctgtccaacg agcccggata ttaccgcgag ggtcactggg gcatccgcat
     1021 cgagaccctg caggtggtca caccgccgac gcccatcccg ggcggcgagc ggccgatgca
     1081 cggcttcgaa cagctgacct tggcgccgct ggaccgccgt ctgattgatg ttccgttgct
     1141 gacgcctgac gagcgcgcct atgtcgacgc ctatcacgcc gaggtgctgg ccaaggtcgg
     1201 tccgttgctc gacggggagg tgagggcgtg gttggaggcg tcctgcgcag cgctgtgaac
     1261 cggctcggcc tgatcgcggt cgccgccgga ctgacggtgg ccgggtgcga tcccgcggaa
     1321 ccgcggacgc agccggttga accgaccccg gaggccatgg cggcggctgt gaatccggcg
     1381 gatatctggt gccttgagcc ggagacgctg cgggtcgaac cgatcgcgtc ggatgcggtt
     1441 tgggcgcgtt ggcgccactg ggccatctgc gactacgacg cccgaaaggc cgcgcactac
     1501 cttgatattc tcgtggcccg cgacgacccg gatgccttgc gggcaaaggg tgagagtttg
     1561 aggaatagcg accctgtcga gagcgaccgc ctgcttcgtc gggcagagcg atttggctgg
     1621 cgcgagccga ctttccggga cgagatcaac aaactcgcca caccgccggc ttgaaataag
     1681 gccgaacggc gccc
//
LOCUS       DASVVN010000056         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_2175, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000056 DASVVN010000000
VERSION     DASVVN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF686_00930"
     CDS             complement(<1..215)
                     /locus_tag="VF686_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8660399.1"
                     /translation="MTRRLRLDALPGLVGQELGVSDWWEISQARIDAFAEATEDRQWI
                     HLDVERATAELGAPIAHGFLTLSMLSA"
     gene            complement(220..1170)
                     /gene="glpX"
                     /locus_tag="VF686_00935"
     CDS             complement(220..1170)
                     /gene="glpX"
                     /locus_tag="VF686_00935"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX8660400.1"
                     /translation="MTDVFDRDLTLPAVRVTEAAAIAAFREVGRGDEKQADQAAVDAM
                     RTALNALDIDGRIVIGEGERDEAPMLFIGEKVGRGSGPRVDIALDPLEGTTLTAKAMP
                     NALAVMAWAPDGGMLHAPDTYMDKIAVGPGYAAGVVDLDASPEDNVKALAKAKGVAVN
                     EITALVLDRPRHAEIIAGLRRAGARVMLITDGDVAGVMNTADASTGIDIYLGQGGAPE
                     GVLACAALKCVGGQFQGRLVFRNDEERGRARRLGLTDFDRKYTLDELVPAHAIFAATG
                     VTKGVLLDGVRYEGGQVVTHSIVMNSATRTVSEIRARRPA"
     gene            complement(1214..1537)
                     /locus_tag="VF686_00940"
     CDS             complement(1214..1537)
                     /locus_tag="VF686_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660401.1"
                     /translation="MARRLETVRGERTAYPSFADVPPQPADLRTPAQWAQAVGQVESR
                     GAQVAAWPARNPAWLTDPEGDARRLQAQTRVAPQDMPRADQRQITDAFAQGLRQRTEP
                     PPPIP"
BASE COUNT          236 a          644 c          665 g          263 t
ORIGIN      
        1 atggccgaca acatcgacag ggtcagaaac ccgtgcgcga tcggggcgcc aagctcagcc
       61 gtggcgcgct ccacgtcgag gtggatccac tgccggtcct cggtcgcctc ggcaaaggcg
      121 tcgatgcggg cctgcgagat ctcccaccag tccgagacgc cgagctcctg cccgaccagg
      181 ccgggcagcg cgtcgagacg gaggcggcgg gtcaacggct caggccgggc ggcgggcccg
      241 gatctccgac acggtgcgcg tcgccgagtt catgacgatc gagtgggtga ccacctggcc
      301 gccctcgtag cggacgccgt cgagcagcac gcccttggtg actcccgtgg cggcgaagat
      361 ggcgtgcgcc gggaccagct cgtcgagcgt gtacttgcgg tcgaaatcgg tgaggccgag
      421 gcgccgggcg cggccgcgct cctcgtcgtt gcggaacacc agccggccct ggaactggcc
      481 gccgacgcac ttcagcgctg cgcaggcgag cacgccctcc ggcgcgcccc cctgccccag
      541 gtagatgtcg atgccggtcg aggcgtcggc ggtgttcatc acgccggcga cgtcgccgtc
      601 ggtgatcagc atcacccgcg ccccggcccg gcgcaggccc gcgatgatct cggcgtgacg
      661 cggccggtcg agcaccaggg cggtgatctc attcaccgcc acgcctttgg ccttggccag
      721 cgccttgacg ttgtcctcgg gcgacgcgtc gaggtcgacc acgccagccg cgtagccggg
      781 cccgaccgcg atcttgtcca tgtaggtgtc gggcgcgtgc agcatgccgc cgtccggcgc
      841 ccaagccatc accgccaggg cgttcggcat cgccttcgcc gtcagcgtgg tgccttcaag
      901 cggatcgagc gcaatgtcga cgcgggggcc ggagcccctg cccaccttct caccgatgaa
      961 gagcatcggg gcttcgtcgc gctcgccctc gccgatgacg atccggccgt cgatgtccag
     1021 cgcgttcagc gccgtgcgca tggcgtccac cgcggcctgg tccgcctgct tctcgtcgcc
     1081 ccggccgacc tcgcggaagg cggcgatggc cgcggcctcg gtcacccgta cggcgggcag
     1141 ggtcaggtcg cggtcgaaga cgtcggtcat gcggactccg gaagggctcg ccctcgacag
     1201 gcgggaactg ggctcagggg atcgggggcg gcggctcggt gcgctgccgc aggccttgag
     1261 cgaaggcgtc ggtgatctgg cgctggtcgg cgcgtggcat gtcctgaggc gcgacccggg
     1321 tctgcgcctg aagccggcgg gcgtcaccct ccgggtcggt cagccaggcg gggttccgcg
     1381 ccggccaggc ggcgacctgg gcgccccggc tttccacctg gccgacggcc tgggcccact
     1441 gggccggggt gcgcaggtcg gcgggctggg gcggcacgtc ggcaaagctg ggataggcgg
     1501 tccgctcgcc ccggacggtc tccagccgcc gggccacctc ggcggggatg ggcccctctg
     1561 aggttttggg cgcggcgcac ccggctaggc cggcgagagc ggccccgctt aaggcgagga
     1621 caacccttct gccgtatcca gccgctctcc cggccggatc gcgcgtcagg tgcttcgcgt
     1681 tcatcatcgc ggggctcata tgccatgatc gcggcgcgag gaaggctgga gggctgaaat
     1741 ggccgatgtt gcgacccagg accgttcaag cctggacgcc ctgatctcgc ccgaacagcg
     1801 ccgggcca
//
LOCUS       DASVVN010000057         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_212586,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000057 DASVVN010000000
VERSION     DASVVN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212586"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF686_00945"
     CDS             complement(<1..431)
                     /locus_tag="VF686_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX8660402.1"
                     /translation="MGLTRYMTGVAMAALLAGTAMAQTAPVQTPAAAGTLNPAAEAAP
                     PAAPVAPAFQMSDGILATVNDSVITGFDLRQRMLLLIAMTQVQPTPENIPAIQQQALN
                     LLIDERLQAQELTKYEDLVITDEEVDQELAAMAQEVGAAPAT"
     gene            complement(530..>1504)
                     /gene="lptD"
                     /locus_tag="VF686_00950"
     CDS             complement(530..>1504)
                     /gene="lptD"
                     /locus_tag="VF686_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX8660403.1"
                     /translation="RISGQAEWRRTMISAAGVRWEPFVDVRADVYSTADLPPSLGLED
                     ETISRGRATAGLDISYPLIRRMSDSADLILEPMVQFSASTDPDLDPRIPNEDSQTLEL
                     DQSSLFRTDRFPGYDLLEGGLRFTAGMRATMRWDQGREASLFVGRSLRDAQEPAFRVP
                     VPDAPALLYDPSGLASDTSDWVVQGSFSPSDRIRGWGHATIDSSGEIRRAETAIDGRW
                     GRRNLATVSYIIDRSNPIAGPLNRNYEFLQLSGQQFVYGNWGVTVAGIADLERDVITR
                     SEFGLLFDDDCFRFELGWRRDNTRVRPTGPSEGLYVRLNLATFGGTGY"
BASE COUNT          249 a          505 c          510 g          240 t
ORIGIN      
        1 gtcgccggcg cggcgcccac ttcctgggcc atggcggcca gttcctgatc gacctcctcg
       61 tcggtgatca ccaggtcctc gtatttggtc agttcctgcg cctgcagccg ctcgtcgatc
      121 agcaggttca gggcctgctg ctggatggcc ggaatattct cgggcgtcgg ctggacctgg
      181 gtcatggcga tcagcagcag catgcgctgg cgcaggtcga agcctgtgat cacactgtcg
      241 ttgaccgtgg ccaggatgcc gtcggacatc tggaacgccg gcgcgaccgg agccgccggc
      301 ggcgccgcct cggcggccgg attcagcgtt cccgcagcgg ccggagtctg caccggcgcg
      361 gtctgcgcca tggccgtacc ggccaggagg gcggccatcg ccacgcccgt catataacgc
      421 gtcaaaccca tgtcagtccg tattcctcaa gcggcgttgg cccggatcag atccggatag
      481 tcagtccgcg cgcccccggg ccgcgcggcg tccagcgcct gtcgctgcgt caataccctg
      541 taccgccgaa agtggcgagg tttaggcgca catagaggcc ctcggagggg cccgtcgggc
      601 ggacgcgggt gttgtcgcgc cgccagccga gctcaaaccg gaaacaatcg tcatcgaaca
      661 acagtccgaa ttccgagcgc gtaatcacat cgcgctcaag atcggcgatg ccggcgaccg
      721 tcacgcccca gttgccatag acgaactgct ggcccgaaag ctggagaaat tcatagttac
      781 ggttcaacgg ccccgcgatc gggttggacc ggtcgatgat gtagctcacc gtcgcgagat
      841 tgcgccggcc ccaacgaccg tcgattgcgg tttcggcgcg gcggatctcg cccgaggaat
      901 cgatcgtggc gtgcccccag ccgcggatcc ggtccgacgg cgagaaactg ccctgcacga
      961 cccagtcgga ggtgtccgag gccagacccg acggatcgta cagcagggcc ggcgcgtcgg
     1021 ggacgggaac gcggaacgcg ggctcctgtg catcgcgcag gctgcgaccg acgaacaggc
     1081 tggcttcccg gccctgatcc cagcgcatgg tggcccgcat gccggccgtg aaacgcagac
     1141 cgccctccag caggtcgtaa ccggggaacc ggtcggtgcg gaacagcgaa ctctggtcga
     1201 gctccagggt ctggctgtcc tcgttcggaa tacgggggtc cagatccggg tcggtcgagg
     1261 ccgagaactg gaccattggc tcaaggatca ggtcggcgct gtcgctcatc cgccggatca
     1321 ggggatagct gatgtccagc ccggccgtgg cgcgtccgcg ggagatcgtc tcgtcttcca
     1381 gccccaggct cggcggcagg tcggcggtcg aatacacgtc ggcgcgcaca tcgacgaacg
     1441 gctcccaccg cacgccggcg gcggaaatca tggtccggcg ccattcggcc tggcccgaga
     1501 tacg
//
LOCUS       DASVVN010000058         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_81149, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000058 DASVVN010000000
VERSION     DASVVN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81149"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VF686_00955"
     CDS             <1..1504
                     /locus_tag="VF686_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660404.1"
                     /translation="TDSDTVTISDYMAQLMGLEQRGSVTTDELMVRIHPRYHEAVLHA
                     LRQAQTFGTFDVSFPVADAQGKARWIDARGQARGDRGEEGFTSVLGVALDTTEARRAK
                     AQAQAAESRLRDGIESVSDAFVLFDKQGRLILSNQAFQDAFGFEEGVVRRGAFKQDLN
                     RIAALAITSDQPAAGGRAGAREIELNDGRWLQLVERFTSDGGSVVTAADITAIKRKEA
                     ERQRATERLHVTITELEDREERLSLLARKYEVAMTRAEAANQAKSEFLANMSHELRTP
                     LNAINGFSEIMAGEMFGPLGDARYKGYAADILKSGQHLLSLINDILDMAKIEAGKLTL
                     HYETVSLKEVVEDATRLMRGKIQEAGLNLIVDAPEMPEIDADYRGLKQVVLNLISNAV
                     KFTPEGGDIVVALSREDDDRVRVAVTDTGIGIAAKDLDRLARPFEQVEGQHSKTTQGT
                     GLGLALTKSLIELHGGELTIASEPGRGTTVGFVLPIRRPEGVQTEQARAA"
     gene            complement(1510..2010)
                     /locus_tag="VF686_00960"
     CDS             complement(1510..2010)
                     /locus_tag="VF686_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX8660405.1"
                     /translation="MTSKTLKSALAVSVALNLFAIAAGATLFVTRAHVDKRVEAQQTA
                     PRTGSPMLLIGRLDPAVRDRVRDTLRASALEVRPDFEEARQKRREAVAIASSDTFDSA
                     RITTLLAQSREAELRGRARLEVDAVAVLATLEPADRKALSEILTRRGRVVVRGDNEKK
                     APPPAN"
     gene            complement(2007..2435)
                     /locus_tag="VF686_00965"
     CDS             complement(2007..2435)
                     /locus_tag="VF686_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660406.1"
                     /translation="MTSERFLALIAAYGADARRWPESERAAAQAFAAAEPAAVRAALA
                     EADAVDALLHLSRAAQPSMALRDRVIASAAEAGLKARREGRRWLDRLALALGAGWAVA
                     ACAGVAAGVMLTTHLTADAQADAVLYQASLLAVDDTEVLG"
     gene            complement(2441..>2874)
                     /locus_tag="VF686_00970"
     CDS             complement(2441..>2874)
                     /locus_tag="VF686_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8660407.1"
                     /translation="AAEAEDVAQEAMLRAWKQAPRWTPGQAKFDTWLHRVGLNLCYDR
                     LRRRREVPTEAPPDRPDEGPSPDRGLMAADVGARVNAALAHLPDRQREAVVLCHYQEL
                     TNIEAAALMEISVDALESLLSRGRRALRLALADIRPGAEGV"
BASE COUNT          467 a          993 c         1021 g          393 t
ORIGIN      
        1 caccgacagc gatacggtca ccatctcgga ctatatggcc cagctgatgg gactggagca
       61 gcgggggtcg gtgacgaccg acgagctgat ggtccgcatc catccgcgtt atcacgaggc
      121 ggtgctgcac gccctgcggc aggcccagac gttcggcacc ttcgacgtca gtttcccggt
      181 ggccgacgcc caaggcaagg cgcgctggat agacgcgcgc ggccaggcgc ggggcgaccg
      241 gggcgaggag ggcttcacca gcgtgctggg cgtggcgctg gacacgaccg aggcgcgccg
      301 cgccaaggcc caggcccaag cggccgaaag ccggctgcgc gacgggatcg agagcgtgtc
      361 ggacgccttc gtcctgttcg acaaacaggg ccggctgatc ctgtccaacc aggcgttcca
      421 ggacgccttc ggcttcgagg agggggtcgt ccgccggggg gccttcaaac aggacctgaa
      481 ccggatcgcg gccctggcca tcacctcgga ccagccggcg gcgggtggac gggccggcgc
      541 gcgtgagatc gagctgaacg acggccgctg gctgcagcta gtcgagcgtt tcacctccga
      601 cggcggttcg gtggtgacgg cggcggacat caccgccatc aagcgcaagg aggccgagcg
      661 ccagcgggcc accgaacggc tgcacgtgac catcaccgag ctggaagacc gcgaggaacg
      721 gctgtcgctg ctggcccgca aatatgaagt ggccatgacc cgggcggaag cggccaacca
      781 ggccaagtcg gagttcctgg ccaatatgag ccatgagctg cggacgccgc tcaacgccat
      841 caacggcttc tcggagatca tggcgggcga gatgttcggg cctctgggcg acgcccggta
      901 caagggctac gccgccgaca tcctgaagtc gggccagcac ctgctgagcc tgatcaacga
      961 tatcctcgac atggccaaga tcgaggccgg caagctgacg ctgcactatg agacggtgtc
     1021 gctgaaggag gtggtcgagg acgccacgcg gctgatgcgc ggcaagatcc aggaggccgg
     1081 gctgaatctg atcgtcgacg ccccggagat gcccgagatc gacgccgact atcgcggcct
     1141 gaaacaggtg gtgctgaacc tgatctcgaa cgcggtgaag ttcacgcccg agggcggcga
     1201 catcgtcgta gccctgtcgc gcgaggatga cgaccgggtt cgtgtggcgg tcaccgacac
     1261 cggcatcggc atagcggcaa aagacctgga ccggctggcg cgacccttcg aacaggtcga
     1321 gggccagcac tcaaagacca cccaggggac cggcctgggc ctggccctga ccaaatcgct
     1381 gatcgagttg cacggcgggg aactgaccat cgccagcgag cccggacgtg gcaccacggt
     1441 gggcttcgtc ctgcccatcc ggcgccccga gggcgtgcag accgaacagg cccgcgccgc
     1501 gtgagggctt cagttggcgg gaggcggggc cttcttctca ttgtcgccgc gcaccacgac
     1561 gcggccgcgg cgggtcagga tttccgacag ggccttgcga tccgccggct ccagcgtggc
     1621 cagcacagcg accgcgtcaa cctcgagccg ggcgcggccc cgcagctcgg cttcgcgcga
     1681 ctgggccagc agggtggtga tgcgggcgct gtcgaaggta tcggacgagg cgatggcgac
     1741 cgcctcccgc cgcttctggc gggcttcctc gaagtccggc cggacttcca gggccgaggc
     1801 gcgcagggtg tcgcgcaccc ggtcgcgcac ggcgggatcc agccggccga tcaacagcat
     1861 gggcgagccc gtgcgcggcg cggtctgctg ggcctcgacc cgtttgtcga catgagcccg
     1921 ggtcacgaac agcgtcgcgc ccgctgcgat ggcgaacagg ttgagcgcca ccgagacggc
     1981 cagcgcgctc ttcagcgttt tcgacgtcat cccaggacct ccgtgtcgtc caccgccagc
     2041 agcgacgcct gatacaggac cgcgtctgcc tgggcgtcgg cggtcaggtg ggtggtcaac
     2101 atgacgcccg cagccacgcc cgcgcaggcc gccacggccc agccggcgcc cagcgccaag
     2161 gcgaggcggt ccagccagcg gcggccttcg cgacgggcct tcaatccggc ctcggccgcc
     2221 gaggcgatga cgcggtcgcg gagggccatc gagggctggg cggcgcgcga caggtgcagc
     2281 agggcgtcga ccgcgtccgc ctcggccagt gcggcgcgca cggctgcggg ctcggcggcg
     2341 gcgaaggcct gggccgcggc gcgttcgctc tcgggccagc gccgtgcatc ggcgccgtaa
     2401 gccgcgatca gtgcgagaaa ccgttccgac gtcatcacgc tcaaactcct tcagctcccg
     2461 gccggatatc ggccagggcc agtctcaacg cccgccgtcc acgcgacagc aggctttcca
     2521 gcgcatcgac gctgatctcc atcagggccg cggcctcgat attggtcagc tcctgatagt
     2581 ggcacagaac caccgcctcg cgctgacgat ccggcagatg cgccaacgcc gcgtttacgc
     2641 gggcgcccac gtccgcggcc atcaaacccc ggtccggcga cggtccctcg tcgggccggt
     2701 ccggcggcgc ctcggtcggg acctcgcgac gccggcgcag ccggtcgtag cagaggttca
     2761 gacccacccg gtgcagccag gtgtcgaact tggcctggcc cggcgtccag cggggcgcct
     2821 gtttccacgc cctgagcatg gcctcttggg ccacgtcctc ggcctcggcg gcgt
//
LOCUS       DASVVN010000059         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_291502,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000059 DASVVN010000000
VERSION     DASVVN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291502"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF686_00975"
     CDS             <1..251
                     /locus_tag="VF686_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660408.1"
                     /translation="ALGIAIGTAIGIAMGNVAIGIGVGLSIGLALGAGLDSRAGARRR
                     NDGSDGGGTHAPGSSGGGKDADGSDGGSDGGGDGGGGD"
     gene            complement(248..1246)
                     /locus_tag="VF686_00980"
     CDS             complement(248..1246)
                     /locus_tag="VF686_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEX8660409.1"
                     /translation="MEVGADQPPLSVWVVSDGRTGIENQALGLAEAVQRLFPSDITIK
                     RIRWRTAFDRFPSALKAPWMLDPSSDPVNPGAGEAWPDLWIATGRATLPLSAAVRRLS
                     GGRTFVVQTQDPRWRNSAYDLIVAPAHDRIEGPNVLSISGSPHRVTRDRLIDGSAAFA
                     DQLTALPRPRVAALIGGRSRAFDLPEAHAADLAGRIAGAVEAAGGSLMLTFSRRTPEA
                     AKAAMTARLAHLPGLIWDGAGANPYFAFLNFADHVLVTEDSANMAAEAASTGKPVHVL
                     PMIPLKPADKFARLHADLRERGAARPFDGTLERWSYEPLNETERAARAVLERMGRA"
     gene            complement(1237..1710)
                     /gene="greA"
                     /locus_tag="VF686_00985"
     CDS             complement(1237..1710)
                     /gene="greA"
                     /locus_tag="VF686_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8660410.1"
                     /translation="MEKVPMTAEGYRVLDDQLKQLKSVERPSVISAISEAREHGDLSE
                     NAEYHAAKERQGWIEGQIADIEDKISRAQVIDVSKLNGSQVKFGATVTVVDEDTEEEA
                     RYQIVGEHEADVKAGKISIASPIARAMISKEIGDVVEVNTPGGVKAYEILKVEWK"
     gene            complement(1847..2062)
                     /locus_tag="VF686_00990"
     CDS             complement(1847..2062)
                     /locus_tag="VF686_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660411.1"
                     /translation="MRPRTVQVIAALIAAVAFVLAFMAAGIVLVSSLFMALGLAAVAW
                     LAWSLVGRVKLWGSIQQIRERRRKAAT"
     gene            complement(2152..>2256)
                     /locus_tag="VF686_00995"
     CDS             complement(2152..>2256)
                     /locus_tag="VF686_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8660412.1"
                     /translation="YGPDKPRLKAFLQTIHARPAYQRALQRGGPYSYA"
BASE COUNT          350 a          743 c          784 g          379 t
ORIGIN      
        1 tggcgctggg catcgctatc ggcaccgcca ttggcatcgc catgggtaat gtggcgatcg
       61 gcatcggcgt cggactcagc atcggtctgg cgctgggggc cgggctggac agccgcgcag
      121 gcgcccgacg caggaacgat ggttctgatg gcggcggaac acacgccccg gggagcagtg
      181 gaggcggcaa ggacgccgac ggctctgacg gcggatccga tggcggtgga gatggcggcg
      241 gcggggacta ggcccgcccc atccgctcca gcaccgcccg ggccgcccgc tcagtctcgt
      301 tcaacggctc gtagctccag cgctccagcg tcccgtcgaa cggccgtgcg gcacctcgct
      361 cgcgcaggtc ggcgtgcagg cgggcgaact tgtccgccgg cttcagcggg atcatcggca
      421 ggacgtgcac cggcttgccg gtcgaggccg cctcggccgc catattggcg ctgtcctcgg
      481 tgaccagcac atggtcggcg aagttcagga aggcgaaata ggggttcgcg cctgcgccgt
      541 cccagatcag gcccggcaga tgcgccagac gcgccgtcat cgccgccttc gccgcctcgg
      601 gcgtgcgcct tgagaaggtc agcatcaatg aaccgccagc ggcctcgacg gctccggcga
      661 tccgcccggc cagatcggcg gcatgggctt ccggcaggtc gaaggccctt gagcgtcccc
      721 cgatcagcgc cgcgacacgg ggacggggca gggcggtcag ctgatccgcg aaggccgccg
      781 atccgtcgat caggcggtcg cgcgtgaccc gatgcggcga tccgctgatc gacagcacat
      841 tcgggccctc gatccggtcg tgggccgggg cgacgatcag gtcgtaggca gagttgcgcc
      901 agcgcgggtc ctgggtctgg accacgaagg tccggccgcc cgacagccgc cgcacagccg
      961 ccgacagcgg cagggtcgcg cggcccgtcg cgatccagag atcgggccat gcttcgccgg
     1021 cgccggggtt caccgggtcg gacgacgggt ccagcatcca cggcgctttc agcgccgaag
     1081 gaaacctgtc gaaggcggtc cgccagcgga tgcgtttgat cgtgatgtca gatggaaaaa
     1141 ggcgctggac ggcttccgcc agacccagcg cctgattttc gataccggtc cggccgtcgg
     1201 agacgaccca gacggagagc ggaggctggt cggcccctac ttccactcga ccttgaggat
     1261 ttcgtaggcc ttgacgccgc cgggggtgtt cacctcgacc acgtcgccga tctctttcga
     1321 aatcatggcg cgcgcgatgg gcgaggcgat cgagatcttg cccgccttca cgtcggcttc
     1381 gtgttcgccg acgatctggt agcgggcctc ttcctcggtg tcctcgtcga ccacggtgac
     1441 cgtggcgccg aacttcacct ggctgccgtt cagcttggag acgtcgatga cctgggcgcg
     1501 gctgatcttg tcctcgatgt cggcgatctg gccctcgatc cagccctgac gctccttggc
     1561 ggcgtgatac tcggcgtttt cagacagatc gccatgctcg cgcgcttccg agatggccga
     1621 gattacgctc ggccgctcca cggacttcaa ttgcttcagc tgatcgtcga ggacacggta
     1681 gccctcggcc gtcatcggca ctttttccat atgtcgtgaa tttctgagct atcgcccgac
     1741 tagagatttg tgaggccaag gatcgctggg cgctcgcgac cggcccaaga gggtagggcg
     1801 cccggcccca aggatcaagg ggcaagctgt gtccggcaga ttgccgttat gtggcagcct
     1861 tgcgccggcg ctcgcggatt tgctggatgc tgccccacaa tttgacccgt ccaaccaggc
     1921 tccaggccag ccaggcgacg gcggccagac ccagggccat gaacaggctg gacaccagaa
     1981 cgatgcccgc ggccatgaag gcgaggacga acgcgacggc ggcgatcagg gcggcgatga
     2041 cttgtacggt tctcgggcgc atggggctgt taacgttcca ttcagggccg ggttgcgccc
     2101 ctttccggtt tcgcgacgct tggcgaccgg cctgtggcgc cgaaaccatc gtcaggcgta
     2161 gctgtacggc ccgccccgtt gcagggcgcg ctgatacgcc ggccgggcat ggatggtctg
     2221 caggaaggcc ttgagacgcg gcttgtccgg accgta
//
LOCUS       DASVVN010000060         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_475642,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000060 DASVVN010000000
VERSION     DASVVN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475642"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF686_01000"
     CDS             complement(<1..248)
                     /locus_tag="VF686_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8660413.1"
                     /translation="MSVGSLPRRLKVAGAVCAAAALLSGCISASAGPNGQYASPIGDA
                     PVTANPTPYSAALYCMADYARRYSLPSPRIAVGRISDYT"
     gene            complement(245..679)
                     /gene="hfaA"
                     /locus_tag="VF686_01005"
     CDS             complement(245..679)
                     /gene="hfaA"
                     /locus_tag="VF686_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaA"
                     /protein_id="HEX8660414.1"
                     /translation="MNSYIKLVPALALVVALAAPVAASAQSAGSSSMGTYQAGYGASR
                     YTTARPQTGSTRDANGNRLIIDGIIQAGASSYSSASGGVSSSFSGAGGNGSSAIGGST
                     AIGNNLNVVVQGNHNTVIVNSTQVNNGAINAGTSLNGTLRLP"
     gene            848..1498
                     /locus_tag="VF686_01010"
     CDS             848..1498
                     /locus_tag="VF686_01010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8660415.1"
                     /translation="MFNAPLVAVGVAVSIPVLFWFQLRLPDVGQSLAFRPASLWDGGW
                     WPDLLTSMFIHGNWPHAGMNAALALAFGAPVARLMVGLKGAAGFLAFYIVCGLLAGVG
                     YAIVHPALYEDVGGASGAVFGLTGAALRLLGRKSGKLRLLTDKRFLVPAAAIMAVNAA
                     LGLLGFAPGMEGMKIAWEAHAFGFVAGALLIGPWVSVFRARPEAFDSPPDLRDPEA"
     gene            1546..1974
                     /locus_tag="VF686_01015"
     CDS             1546..1974
                     /locus_tag="VF686_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8660416.1"
                     /translation="MLVAEILKDKGDAVYAIGPELTVSEACGELDRLRVGALMVCRND
                     KVVGVLSERDVVKAVARDGQAALNQPVSAVMTSEVVFAAPGETVAILMGRMTDRRIRH
                     LPVLRDGQLAGVISIGDVVKCQIAEATQEAESLRTYIAAG"
BASE COUNT          291 a          609 c          729 g          380 t
ORIGIN      
        1 gtgtagtcgc tgatccggcc gaccgcgatg cgcggcgagg gcaggctgta gcggcgggca
       61 tagtcggcca tgcaatagag tgcggcggag tagggggtcg ggttggccgt cacgggcgcg
      121 tcgccgatcg gcgaggcgta ctgtccgttg gggccggccg aggccgagat acagccgctg
      181 agcagcgccg cggccgcgca gactgcgccg gcgactttga gcctgcgggg aagggagccg
      241 acgctcatgg aagcctcagg gtgccgttca ggctggttcc ggcgttgatc gcgccgttat
      301 tgacctgggt ggagttgacg atgaccgtgt tgtggttgcc ctgaacgacg acgttcaggt
      361 tgttgccgat ggcggtcgag ccgccgatgg cgctgctgcc gttgccgccg gcgcccgaga
      421 agctggagga aactccgccg ctggcgctgg agtagctgct ggcgccggcc tggatgattc
      481 cgtcgatgat cagccggttg ccgttggcgt cccgcgtcga tcccgtctgc gggcgcgccg
      541 tggtgtagcg cgaggcgccg tacccggcct gataggttcc catgctcgac gagcccgccg
      601 actgggccga ggccgcgacg ggggcggcga gggcgaccac aagggcaagc gcaggcacga
      661 gcttgatgta gctgttcatg gcgaccacct ctcacgcgga caaagtcaac ggaccggtaa
      721 ggatccttcg tcccactctg ggtcatggtt attaagaagc gcttgccatg atggcgtccg
      781 acaaccgatt ggtttgtctt tgtgaatccc gggagctttc gccctgaacg cgcaaccgtc
      841 cgaacgtgtg ttcaacgccc cgctggtcgc ggtcggcgtc gccgtgtcga ttccggtgtt
      901 gttctggttc cagctgcgcc tcccggatgt tgggcaatca ctcgccttcc gtcctgcctc
      961 tctttgggat ggcgggtggt ggccagacct gctgaccagc atgttcatcc acggcaactg
     1021 gcctcacgcc ggaatgaacg cggcgctggc gctggccttc ggggcgcccg tggcgcggct
     1081 catggtcggt ctgaaggggg cggcaggttt tctcgccttc tatatagtgt gcggcctact
     1141 ggccggcgta ggctacgcga tcgttcatcc ggccctctac gaggacgttg gaggcgcgtc
     1201 cggtgcggtg tttggtttga cgggcgctgc ccttcgtctg ctcggccgga agtccgggaa
     1261 gctgagactc ctgaccgaca agcgctttct ggtccccgcg gcggccatca tggccgtcaa
     1321 cgccgctctt ggcctgctgg gctttgcccc cggaatggag gggatgaaga tcgcctggga
     1381 ggcccacgcc ttcggtttcg tcgccggagc cctgctgatt ggtccgtggg tcagcgtatt
     1441 tcgcgcaagg ccggaagcat ttgattcgcc gcccgatctg cgcgaccctg aggcctgaag
     1501 cggtccttcc gcggccgcac tctatataga gagggaggag ccgcgatgct ggtcgccgaa
     1561 attctcaagg acaagggcga cgccgtctac gcgatcgggc ccgaactgac ggtcagcgag
     1621 gcgtgcggcg aactggatcg gctgcgcgtc ggcgccctga tggtctgcag gaatgacaag
     1681 gtggtgggcg tgctgtccga gcgcgacgtg gtcaaggccg tggcccgcga cggtcaggcg
     1741 gctctgaatc agcccgtttc ggccgtcatg acctccgaag tggtgttcgc ggcgcccggc
     1801 gagacggtgg cgattctgat gggacggatg accgaccgcc gaattcgcca tctgccggtg
     1861 ctgcgcgacg gtcagctggc gggcgtcatc tccatcggcg acgtggtgaa gtgccagatc
     1921 gccgaggcca cccaggaagc ggaatcgctg cgcacctata tagcggcggg ctgaaacgca
     1981 ggcggggcgg gccctgtcac ggcctgccg
//
LOCUS       DASVVN010000061         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_265488,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000061 DASVVN010000000
VERSION     DASVVN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265488"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /gene="sucA"
                     /locus_tag="VF686_01020"
     CDS             <1..354
                     /gene="sucA"
                     /locus_tag="VF686_01020"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515717.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX8660417.1"
                     /translation="VMCSGKVYYDLLEEREKRGIDDVYLLRLEQFYPWPMKSLMTELA
                     RFPNAELVWCQEEPKNMGGWTFVDPWIELTLEKLNVKSKRARYVGRPASASTAAGLMS
                     RHLKELDAFLTDAFA"
     gene            420..779
                     /locus_tag="VF686_01025"
                     /pseudo
     CDS             420..779
                     /locus_tag="VF686_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            801..1991
                     /gene="odhB"
                     /locus_tag="VF686_01030"
     CDS             801..1991
                     /gene="odhB"
                     /locus_tag="VF686_01030"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX8660418.1"
                     /translation="MTPTMGESVAEGSMGTWLKKSGDTVAKDEMLVEIETDKVAVEVS
                     APAAGVLTIVAEAGSTVTPGQQIGSIAASGAAAAPAPAAAPASGGSANAGSAQIKSGD
                     PHLSPAVQRVVTENNLDPKALAATGPKGNITKADAVAAIGAAAPAPAAAAPAAAPAAP
                     RADQPREERVKMTRLRQTIARRLKESQNTAAQLTTFNEVDMTNIMALRNQYKEVFEKR
                     HGVKLGFMSFFTKAIVAALKEIPAVNAEIDGMDIIYKNHYDIGVAVGTDKGLVVPVLR
                     DADTLSLAGIEKGIAALGKAARDGALTLDQLQGGTFTITNGGTYGSLMSTPILNAPQS
                     GILGMHNIVQRPMAVNGEVLIRPMMYLALSYDHRIVDGKEAVTFLVRVKELLEDPARA
                     LLDL"
     gene            2077..>2460
                     /locus_tag="VF686_01035"
     CDS             2077..>2460
                     /locus_tag="VF686_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660419.1"
                     /translation="MSLHEIARFTDVYEADLAAAFLESQGIRVFVTERHQTTIDPLMQ
                     RALGIRLLGAPSNVAEARSLLSRARAGEFATDEGDDIQPMDKGTRAAGAVMAIAAFAT
                     GGFWGNSLPRRFRVPPWQGMLIAIAL"
BASE COUNT          428 a          904 c          790 g          338 t
ORIGIN      
        1 gtgatgtgct cgggcaaggt ctactacgac ctgctggagg agcgggagaa gcgcgggatc
       61 gacgacgtct acctgctgcg cctcgagcag ttctatccgt ggccgatgaa gtcgctgatg
      121 accgaactgg cccgcttccc caacgccgaa ctggtctggt gtcaggaaga gcccaagaac
      181 atgggcggct ggaccttcgt cgacccgtgg atcgaactga cgctcgagaa gctgaacgtg
      241 aagtccaagc gcgcccgcta cgtgggccgc cctgcctcgg cctcgaccgc cgcgggtctg
      301 atgagccggc acctgaaaga actcgacgcc ttcctgaccg acgccttcgc ctgaacccat
      361 tccggactgc ctcgcggcgg ccggaatgac caacgaacga acctgagaga gcccgtccca
      421 tggccgacat cctcaccccc gccctcggcg aatccgtcac cgaagccacc atcggcaagt
      481 ggacgaagaa ggccggcgat ccggtgaaga aggacgaggt cctggtcgag ctggaaaccg
      541 acaaggtctc gctcgaagtg gtttcgccct cggacggcgt cctgtccgag atcaaggctc
      601 ccgacggcga gaccgtgacc cccggcgccg tgctgggcgt ggtcaccgaa ggcgccgccg
      661 ccgccccggc cgccgaggct cccaaggctg aagccaaggc cgcgcccgct gcagccgctc
      721 cggccgctga acccaccccg gccgctgctc ccgccgctgc accggccccg gccgccgcct
      781 cgggcggcgc cgtgccgatc atgaccccga ccatgggcga aagcgtcgcc gagggctcga
      841 tgggaacctg gctgaagaag tcgggcgaca ccgtcgccaa ggacgagatg ctggtcgaga
      901 tcgagaccga caaggtcgcg gtggaagtgt ccgcccccgc cgccggcgtc ctgaccatcg
      961 tggctgaggc cggctcgacc gtcaccccgg gccagcagat cggctcgatc gccgcctcgg
     1021 gtgcagccgc cgccccggct cccgccgccg cccccgcatc aggcggttcg gccaacgccg
     1081 gctcggccca gatcaagtcg ggtgaccccc acctgtcgcc cgccgtccag cgcgtggtga
     1141 ccgagaacaa cctcgatccg aaggcgctcg ccgccacggg gcctaagggc aacatcacca
     1201 aggccgacgc cgtcgccgcc attggcgccg ccgcaccggc tccggccgcc gccgcccccg
     1261 ccgctgcccc cgccgccccg cgcgccgacc agccgcgtga ggagcgggtc aagatgaccc
     1321 gcctgcgcca gaccatcgcg cgccgcctga aggaatcgca aaacaccgcc gcccagctga
     1381 ccaccttcaa cgaggtggac atgaccaaca tcatggccct gcggaaccag tacaaagagg
     1441 tgttcgagaa gcgccacggc gtgaagctgg gcttcatgag cttcttcacc aaggcgatcg
     1501 tcgccgccct gaaggagatc ccggccgtca acgccgagat cgacggcatg gacatcatct
     1561 acaagaacca ctacgacatc ggcgtcgccg tcggcaccga caaggggctg gtcgtcccgg
     1621 tcctgcgcga cgcggacacc ctgtcgctgg cgggcatcga gaagggcatc gcggcgctcg
     1681 gcaaggcggc ccgcgacggc gccctgaccc tggaccagct acagggcggc accttcacca
     1741 tcaccaacgg cggcacctat ggctcgctga tgtcgacgcc gatcctgaac gcgccgcagt
     1801 ccggcatcct cggcatgcac aacatcgtcc agcgccccat ggcggtgaac ggcgaggtgc
     1861 tgatccgtcc gatgatgtac ctggccctca gctacgatca ccgcatcgtc gacggcaagg
     1921 aggcggtcac tttcctcgtc cgcgtcaagg aactgctgga agacccggcc cgggcgctgc
     1981 tggacctgta gaaatcgatc cggatcggaa tgaatgcggc gggcgtcccg ggacgcccgc
     2041 cgtttccaat tggcggcagc gtgctagctt cgccgtatga gcctccacga gatcgcacgt
     2101 ttcaccgacg tctacgaggc cgatctcgcc gcggcgtttc tggaatcgca gggcatccgg
     2161 gtcttcgtga ccgagcgtca ccagaccacc atcgaccccc ttatgcagcg ggcgctgggc
     2221 atccgtctgc tgggcgcgcc ctcaaacgtc gcggaggcgc ggtccctgct gagccgggcc
     2281 cgcgctggag agttcgccac cgacgaaggc gacgacatcc agcccatgga caagggaacg
     2341 cgcgccgccg gcgccgtgat ggccatcgcc gccttcgcga ccggtggctt ctggggcaac
     2401 agcctgccgc gccggttccg ggtgccgccg tggcagggga tgctgatcgc catcgccctg
//
LOCUS       DASVVN010000062         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_475748,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000062 DASVVN010000000
VERSION     DASVVN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475748"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2108)
                     /locus_tag="VF686_01040"
     CDS             complement(<1..>2108)
                     /locus_tag="VF686_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX8660420.1"
                     /translation="KLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVL
                     MMSTNNILSPANGRPIIVPSQDIVLGLYYLSLAKDGEPGEGKLFADIGEIEAALAEGC
                     VTLHSKVKARFTESDPDGTIRARVIDTTPGRMKIAALFPRHPQIGHRLIEKPLTKKEI
                     GNLIDMVYRHCGQKATVIFADQIMGLGFREAAKAGISFGKDDIVIPERKKPIVEETRK
                     LVEEYEQQYADGLITKGEKYNKVVDAWAKATDRVADEMMGEISGSGTGGGEVNSIYMM
                     AHSGARGSQAQMKQLGGMRGLMAKPSGEIIETPIISNFKEGLTVLEYFNSTHGARKGL
                     ADTALKTANSGYLTRRLVDVAQDSIITEEDCGGTRGITVRAVVEGGDTLVSLGQRILG
                     RTLAEDVREPGGEAIVVKADTYLDEVLIDKIEKAGVTSVKVRSVLVCEAETGVCAACY
                     GRDLARGTRVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGTAQVAETSFFESANNGVV
                     RFVGANTVEASDGALVVMNRNTQLVVSVDGKDRETYKPPYGARLRVKDGGDVRKGQRL
                     ADWDPYTSPIITEVGGRVRAEDLVEGVSVREEADEATGISNRVVIDWRASPRGTDLRP
                     AMGVLATSDSGYQKLSNGGDARYLLPVGAILSVADGDEAKPGDVLARIPTEGAKTRDI
                     TGGLPRVAELFEARRPKDCAV"
BASE COUNT          266 a          703 c          757 g          382 t
ORIGIN      
        1 accgcgcagt ccttgggccg gcgcgcctcg aacagctccg ccacgcgcgg caggccgccc
       61 gtgatgtcgc gggtcttggc gccctcggtc gggatgcggg ccagcacgtc gccgggcttc
      121 gcctcgtcgc cgtcggccac cgagaggatg gcgcccacgg gcaggaggta gcgcgcgtcg
      181 ccgccgttcg acagcttctg gtagccgctg tcgctggtgg ccaggacgcc catggccgga
      241 cgcaggtccg tgccgcgggg gctggcgcgc cagtcgatga ccacgcggtt ggagatgccg
      301 gtggcttcgt cggcctcctc gcggacgctg acgccttcca ccaggtcctc ggcgcggacg
      361 cggccgccga cctcggtgat gatcgggctg gtgtaggggt cccagtcggc caggcgctgg
      421 cccttcctga cgtcgccgcc gtccttgacg cgcaggcgcg cgccgtaggg cggcttgtag
      481 gtctcgcggt ccttgccgtc gacgctgacc accagctggg tgttgcggtt catgacgacg
      541 agcgcgccgt ccgaggcctc caccgtgttg gcgccgacga agcggacgac gccgttgttg
      601 gcggactcga agaacgaggt ctccgccacc tgcgcggtgc ccccgatgtg gaaggtgcgc
      661 atggtgagct gggtgcccgg ctcgccgatc gactgggcgg cgatgacgcc caccgcctcg
      721 ccgatgttga cccgcgtgcc gcgcgccagg tcacggccgt agcaggccgc gcagacgccg
      781 gtctcggctt cgcagacgag cacggagcgg accttcaccg aggtgacgcc ggccttctcg
      841 attttgtcga tgaggacttc gtcgagatag gtgtcggcct tgaccacgat cgcctcgccg
      901 cccggctcgc ggacgtcttc ggccagggtg cggccgagga tgcgctggcc cagcgagacc
      961 agcgtgtcgc cgccctcgac caccgcgcgg acggtgatgc cgcgggtgcc gccgcaatcc
     1021 tcttccgtga tgatggagtc ctgggcgacg tcgacgagac ggcgcgtcag gtagccggag
     1081 ttggcggtct tcagggcggt gtcggccagt cccttacggg cgccgtgggt ggagttgaag
     1141 tactccagca cggtcaggcc ttccttgaag ttcgagatga tcggcgtctc gatgatctcg
     1201 cccgacggct tggccatgag gccgcgcatg ccgccgagct gcttcatctg ggcctggctg
     1261 ccgcgggcgc cggagtgggc catcatgtag atggagttca cttcgccgcc gcccgtgccg
     1321 gagccggaga tctcgcccat catctcgtcg gcgacgcggt cggtcgcctt ggcccaggcg
     1381 tcgaccacct tgttgtactt ctcgcccttg gtgatcaggc cgtcggcgta ctgctgctcg
     1441 tactcttcca cgagcttgcg ggtttcctcg acgatcggct tcttgcgctc ggggatgacg
     1501 atgtcgtcct tgccgaagct gatccccgcc ttggcggcct cgcggaagcc caggcccatg
     1561 atctggtcgg cgaagatgac cgtcgccttc tggccgcagt gccggtagac catgtcgatc
     1621 aggttgccga tctccttctt ggtcagcggc ttctcgatca ggcggtggcc gatctgcggg
     1681 tggcgcggga acagggccgc gatcttcatg cggccgggcg tggtgtcgat gacgcgggcc
     1741 cggatcgtgc cgtcggggtc ggactcggtg aagcgggcct tcaccttgga gtgcagggtg
     1801 acgcagcctt cggccagcgc cgcctcgatc tcgccgatgt cggcgaacag cttgccctcg
     1861 cccggctcgc cgtccttggc cagcgacagg tagtagaggc ccagcacgat gtcctgcgac
     1921 ggcacgatga tcgggcggcc gttggcgggg ctgaggatgt tgttggtcga catcatcagc
     1981 acacgggctt ccagctgcgc ctcgaggctc agcggcacgt gcacggccat ctggtcgccg
     2041 tcgaagtcgg cgttgaaggc ggcgcagacc agcgggtgca gctggatggc cttgccctcg
     2101 atcagctt
//
LOCUS       DASVVN010000063         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_54924, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000063 DASVVN010000000
VERSION     DASVVN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54924"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF686_01045"
     CDS             <1..640
                     /locus_tag="VF686_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660421.1"
                     /translation="AAAQVDLPRSRADNAALASAQKEPLGARLRGVLSQDPASRRVLE
                     VADASPVPVLAPADPNLLRTARIFTGDRHYMLVVQRGEQIIEIYGATKAFQSPIAPAT
                     QPPPTRPPTPGTLAAARPTAPASTLARAGPRIAAARVAPAASRAAVEARAEGLTNVRT
                     ERTEYGMDVTFSRFGAAYNVSFICEGPGATGCTEAEAVAFASTLQLIGGGAS"
     gene            637..1491
                     /locus_tag="VF686_01050"
     CDS             637..1491
                     /locus_tag="VF686_01050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8660422.1"
                     /translation="MKRLTLAALLLLTACSPNGCVLEPDAPPVKADPAAPPAIPTEPA
                     PPPGEVPPAPMPEPEPVPETPPAPEPATWPHDPGGALPSGSGTGATDATLWAAGMRFP
                     MEAGPAYANSQVYGYGGFAAPGPGGQCDPRNYAYPWRDNFCETRSWTNGMCPAGRGHQ
                     GQDIRPATCVKKVHWIVAAEKGRITSIGSYTVTLLGDSGRIYRYLHMDMPGVNALFPT
                     MASRNVERGQHIGQVSADFGGNATTIHLHFEIKAPVAIASNPPSVNFVPTYTSLVDSY
                     GRMLNGAA"
BASE COUNT          227 a          590 c          495 g          207 t
ORIGIN      
        1 ggccgcggcc caagtcgatt tgccgaggtc gcgcgccgac aacgccgccc tggcctccgc
       61 gcagaaggaa ccgctgggcg cccggctgcg cggggtgctg agccaggacc cggcgtcgcg
      121 gcgcgttctc gaggtggccg acgcctctcc cgtgccggtg ctggccccgg cggatcccaa
      181 cctgctgcgc accgcccgca tcttcaccgg ggaccgccac tatatgctgg tggtccagcg
      241 cggcgagcag atcatcgaga tctacggcgc gaccaaggcc ttccagtcgc cgatcgcgcc
      301 ggcgacgcag ccgccgccga cccgacctcc cacgccggga accctggccg ccgcccgccc
      361 gacggccccc gcctcaacac tcgcgcgggc cggtccccgc atcgccgccg cccgggtcgc
      421 gcccgccgcc tcgcgcgccg ccgttgaggc ccgggccgag gggctgacca atgtgcggac
      481 cgagcgcacc gaatacggca tggacgtcac cttcagccgc ttcggcgccg cctataatgt
      541 cagcttcatc tgcgaaggcc ccggtgcgac gggctgcacc gaggcggagg ccgtcgcctt
      601 cgcctcgacc ctgcaactga tcggcggagg cgcctcgtga aacggctgac cctcgcagcc
      661 ctgttgctgc tgaccgcctg ttctcccaac ggatgcgtcc ttgagcccga cgccccgccg
      721 gtgaaggccg acccggccgc gccccccgcc atcccgacgg agccagcccc gccgcccggc
      781 gaggtcccgc cggcgcctat gcccgagccg gaacccgtgc cggagactcc gcccgcgcca
      841 gaacctgcca cctggccgca cgacccgggc ggcgccctgc cctcgggctc cggaaccgga
      901 gcgaccgacg ccaccctgtg ggccgccggc atgcggttcc cgatggaggc cgggccggcc
      961 tacgccaact cgcaggtcta cggctacggc gggttcgctg cgccgggacc gggcggccag
     1021 tgcgacccgc gcaactacgc ctatccatgg cgcgacaatt tctgcgagac gcggtcctgg
     1081 accaacggca tgtgccccgc cgggcgcggc catcagggtc aggatatccg ccccgcaacc
     1141 tgtgtgaaga aggtccactg gatcgtcgcg gccgagaagg gacggatcac ctcgatcggc
     1201 agctacaccg tcaccctgct gggcgattcc gggcgcatct accgctatct gcacatggat
     1261 atgccggggg tgaacgccct gttcccgacg atggcttcgc gcaatgtcga gcgtgggcaa
     1321 catatcggac aggtgtcggc ggacttcggc ggcaacgcca ccaccatcca tctgcatttc
     1381 gagatcaagg cgccggtggc gatcgcgagc aatccaccca gcgtcaattt cgtgccgacc
     1441 tacaccagcc tggtggacag ctatggccga atgctgaacg gcgcggcctg agaccgcttc
     1501 ctcagtcgaa cgggcggtt
//
LOCUS       DASVVN010000064         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_370813,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000064 DASVVN010000000
VERSION     DASVVN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370813"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF686_01055"
     CDS             complement(<1..248)
                     /locus_tag="VF686_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8660423.1"
                     /translation="MTDTTTPGPIDGDRYRLLVNAITDYAIYMLDTDGRVASWNPGAE
                     RFKGYTQGEIIGEHFSTFYTHADREIGAPAAALKEAAE"
     gene            complement(334..>1632)
                     /locus_tag="VF686_01060"
     CDS             complement(334..>1632)
                     /locus_tag="VF686_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8660424.1"
                     /translation="ILIDEGKVKWDAPVRTYLPGFSLSKPELNAMVTVRDLVSHRIGF
                     GLGAGDLLFWPNSDRTRAEIMAAIPHVPIEDQFRTTYHYCNLMFVAAGEVVAAASGMP
                     WEAFVQTRILNPLGMTETLPVMTAADPAKSALPHGRIGPPLRYQGEMQTIGQSIQEVW
                     DWAAAGAAGGFVTNPVDWAKWIAVQLARGRLPDGTRLYSEARANEMWRPNILISNSAG
                     PTAELPGRAIASTYATGWQVQDYRGERMITHGGGAPGFVSGTVLIPGRDAGFSYFTNA
                     EESFLTRALRSGITDIVMGKADFDWVADGVRLQAEGDARSVAAAAEIDAKQAAGAPPS
                     LPMSAFAGTWRDPWYGDITIEARDLVLWLKFSRTPSLQGPLEPYDGDTLRTRFTDRRE
                     EDAFISFKIENGVPVSATMRAVSPDADFSYDYHDLRLTRV"
BASE COUNT          249 a          608 c          505 g          270 t
ORIGIN      
        1 tcctcggccg cctccttcag agccgccgcc ggcgctccga tctctcgatc cgcatgggtg
       61 tagaaggtcg agaaatgctc gccgatgatc tcgccttgcg tatagccctt gaaccgctcg
      121 gcccccggat tccagctggc cacgcggccg tcggtatcca gcatgtagat ggcgtagtcg
      181 gttatcgcat tgaccagcag acgatagcgg tcgccatcga tcggcccggg ggtcgtcgtg
      241 tctgtcatca tgccccctga caccgcgcag cccgcgcgaa aggcgcggct tcaacgatcc
      301 ggacgtcccg atggttcctt tgaacgcctt ccgtcaaacc cgcgtcagcc tcaggtcgtg
      361 atagtcgtac gagaagtccg catccggact gaccgccctc atcgtcgccg acacggggac
      421 gccgttctcg atcttgaagc tgatgaaggc gtcctcctcg cgccggtccg tgaaccgggt
      481 ccgcagggtg tcgccgtcat agggctccag cggtccctgc agcgagggcg tccggctgaa
      541 tttcagccaa agcaccaggt cccgcgcctc gatggtgatg tcgccatacc agggatcgcg
      601 ccaggttccc gcaaaggccg acatgggcag agacggcggc gcacccgccg cctgtttcgc
      661 atcgatctcc gccgccgccg cgaccgatct ggcatcgccc tccgcctgca ggcgcacacc
      721 gtcggcgacc cagtcgaagt ccgccttgcc cattacgatg tcggtgatgc cgctgcgcag
      781 cgcccgcgtc aggaagctct cctcggcatt ggtgaaatac gagaagcccg cgtcccgccc
      841 ggggatcagc accgtccccg agacgaagcc cggcgcccct ccgccgtggg tgatcatccg
      901 ctcgccgcga tagtcctgca cctgccagcc cgtcgcatag gtcgaggcga tggcccgccc
      961 cggcagctcg gccgtcggcc ccgccgagtt cgagatcagg atgttgggcc gccacatctc
     1021 gtttgcccgc gcctccgaat acagccgggt cccgtccggc agccgcccgc gcgccagctg
     1081 caccgcgatc catttggccc agtcgaccgg attggtcacg aagccgcccg ccgccccggc
     1141 cgccgcccag tcccagacct cctggatcga ctggccgatg gtctgcatct cgccctgata
     1201 gcgcagcggc ggcccgatcc ggccgtgcgg cagcgccgac ttcgccgggt ccgccgccgt
     1261 catcaccggc agggtctcgg tcatgcccag cgggttcagg atgcgcgtct ggacgaacgc
     1321 ctcccacggc atgcccgagg cggcggcgac cacctccccc gccgccacga acatcaggtt
     1381 gcagtagtgg taggtcgtcc ggaactggtc ctcgatcggc acgtgcggga tggccgccat
     1441 gatctcggcc cgcgtccggt cgctgttggg ccagaacagc aggtcgcccg cgcccagtcc
     1501 gaagccgatc cggtggctga ccagatcgcg caccgtcacc atggcgttca gctcgggctt
     1561 cgacagcgag aagccgggca gataggtccg caccggcgcg tcccacttca ccttgccctc
     1621 gtcgatcagg at
//
LOCUS       DASVVN010000065         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_134323,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000065 DASVVN010000000
VERSION     DASVVN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134323"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1491)
                     /locus_tag="VF686_01065"
     CDS             complement(202..1491)
                     /locus_tag="VF686_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8660425.1"
                     /translation="MAPQGEAGFPATLSTWVREIVGAIPGPAQSTIAELLVGAMLGGG
                     GHVTRALLAVTPRRGWQAYHWMLERGRFRLLGLVAVLCGIVRRETRGPRRFAVIDDTL
                     APRCSAKAPGAAVRFDHARKPNRPAFLLCQAFVTLGAVVPCRDRPRAVPIVTGLCRGA
                     GHAGKIALAKTLLRAVGDRLGPLCLLLDAWYMRGSLIRAALRRGHAVIGQVRRDTALF
                     RVPPPRAPGTRGRPRVYGEKIDAEAAARLPASFHRIAGYGGRMARLRHALCRPRFLRG
                     VVVRAVWCELGKRGGGWAKQRLLLSADTALSASAIVEAYANRWAIEPLFAALKLGDGL
                     GAMWQRGRTALLRWLHLVQIGRALLVLLTARAGPQTLALVRVGGWRRAATLTPGLVKE
                     ALAHRFRDCEVFRLVPAMRGKSGPVRGIGPPTCAAAA"
BASE COUNT          235 a          588 c          575 g          197 t
ORIGIN      
        1 caatccttct gatgattggg ttccttggcc tacgcggtgg cttcaccgcg acggggatac
       61 tctccggctt aatttcgctt tcgccggatc aactgggaac tggagtttcg agtttgcctg
      121 gctgtgcttt gccagccgcc ccggtctgat cggaccgtcc gcatcggttg gaggctgcag
      181 aagaaggcgg ttgcgcggca ctcatgccgc ggcggcacat gtgggtggcc cgatgccacg
      241 aaccggcccg gatttgccgc gcatcgcggg gacgaggcgg aacacctcgc aatcgcggaa
      301 cctgtgggcg agcgcctcct tgacgagacc cggcgtcagc gtcgccgccc ggcgccagcc
      361 accgacccgg accagggcga gggtctgtgg ccccgccctc gcggtcagca ggaccagcag
      421 ggcgcggccg atctgcacca ggtgcagcca gcgcagcagg gccgtccggc cgcgctgcca
      481 catcgcgccg aggccgtcgc cgagcttgag ggcggcgaac agcggctcga tggcccagcg
      541 gttcgcgtag gcctcgacga tcgcgctggc ggacagggcc gtgtcggccg agagcagcag
      601 gcgctgcttc gcccagccgc cgccacgctt ccccagctcg caccagaccg cccgtacgac
      661 gacgccgcga aggaaccgag ggcggcagag ggcatggcgc aggcgcgcca tccggccgcc
      721 gtagccggcg atgcggtgga acgaggccgg caaccgcgcc gccgcctcgg cgtcgatctt
      781 ctcgccatac acacgtgggc gcccacgggt tcccggcgcg cgcggcggcg gcacccggaa
      841 cagggcggtg tcgcgccgga cctggccgat gacggcgtgg ccgcgccgca gggcggctcg
      901 gatcaagctg ccgcgcatgt accaggcgtc gagcagcagg cacagcgggc cgaggcggtc
      961 gcccaccgcc cgcagcaacg tcttggccag ggcgatcttc ccggcatggc cggcgccgcg
     1021 gcacaggccg gtcacgatcg gcaccgcgcg cggccggtcg cggcagggca cgacggcgcc
     1081 gagcgtgacg aaggcctggc acagcaggaa ggccgggcgg ttcggcttcc tcgcgtggtc
     1141 gaaccgcacc gcggcgccgg gcgccttggc cgagcaacgc ggcgccaggg tgtcgtcgat
     1201 cacggcgaag cgtcgtggcc cgcgggtctc gcgccggacg atgccgcaca acacggcgac
     1261 caggccgagc aggcggaagc ggccccgctc cagcatccag tggtaggcct gccagccccg
     1321 ccgcggcgtc accgccagca acgcccgggt gacgtgcccg ccgccgccca gcatggctcc
     1381 gacaagaagc tcggcgatgg tgctctgggc gggccccggg atggccccca cgatctcccg
     1441 cacccatgtg gacagcgtcg ccgggaaccc ggcctcgcct tgcggtgcca tggcgccttc
     1501 gggtggttgg tatgacagcc gcaacatggg gccgctcacc cggcgacacc gccctcaccc
     1561 cgtcagacct ccccaggcca gcccggacta gaaac
//
LOCUS       DASVVN010000066         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_213093,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000066 DASVVN010000000
VERSION     DASVVN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213093"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF686_01070"
     CDS             complement(<1..576)
                     /locus_tag="VF686_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX8660426.1"
                     /translation="MTDTSTAALPDGQALAALIAGKLCHDFISPAGAISSGLDLLKDP
                     SAQDMRDDAMGLIEASAKKMVALVSFARVAFGAATSAERFSGSELGALVAGLTEGGRA
                     TLDWKAEDASYSKAQSRALVNLAYLAMAALPSGGIAAISTRREAGDLIIEGLAEGNRA
                     RLKAEAVAGLAGQPLSEGLPGQWIQPYWLWLT"
     gene            666..1451
                     /gene="cysQ"
                     /locus_tag="VF686_01075"
     CDS             666..1451
                     /gene="cysQ"
                     /locus_tag="VF686_01075"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8660427.1"
                     /translation="MIKRLTADLKSGALTEALADIAEDAARVILPYWRSGVVAETKAD
                     DSPVTQADREAEALILSRLEALYPGVQTVAEEAVAAGGAPAEAAEWFWLIDPLDGTRG
                     FVEGRESFTVNIALIHKGAPVAGVVTAPATATTWRSGPAKGGAFRRRFGEGWQAIKVR
                     NRPASPMAVMSHSMTDAEAERLASRHGCVQWQGTDSSLKFCLIAEGRFDAYPRTGPTS
                     EWDTAAGQAVLEAAGGRVMGEDGQPLRYGKSCFANGGFVALGG"
     gene            complement(1458..>2096)
                     /locus_tag="VF686_01080"
     CDS             complement(1458..>2096)
                     /locus_tag="VF686_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660428.1"
                     /translation="RRDGRLLDQAGRDLRALVEAASPDYRPLTRARALALAGAGLIAL
                     ARLARSDAAAEQGRALFDAAADQFTSDHSPLDWVAVQLVQTPGETPLLTLAQAEALTR
                     EPGLVLGALARERRLATEVDLAEDVGDLAALIGLETAVRQRLTAPAARPLDWAADQIG
                     MARLAMAKGRLMGVEPRAIGLMLAEAMETAREWGAPALLERAMLAMPEKVSL"
BASE COUNT          297 a          738 c          782 g          279 t
ORIGIN      
        1 cgtcagccac agccaatacg gttgaatcca ctgccccggc aggccctcgc tgaggggctg
       61 cccggccaga cccgccacgg cctcggcctt caggcgggcg cggttgcctt cggccaggcc
      121 ctcgatgatc aggtcgccgg cctcgcggcg ggtggagatc gcggcgattc cgcccgaggg
      181 cagggcggcc atggccagat aggccagatt caccagcgcg cgcgactggg ccttggaata
      241 gctggcgtcc tcggccttcc agtccagggt cgcgcggccg ccctcggtca ggcccgccac
      301 cagcgcgccc agttcggagc cggagaagcg ctcggccgag gtcgcggcgc caaaggccac
      361 gcgtgcgaac gacaccagcg ccaccatctt cttcgcgctc gcctcgatca ggcccatggc
      421 gtcgtcgcgc atgtcctggg ccgagggatc cttgagcagg tcgaggccgg aactgattgc
      481 gccggccggg ctgatgaagt cgtggcacag cttgcccgcg atcagggcgg cgagcgcctg
      541 cccgtcagga agggcggcgg tggaggtgtc ggtcataggg ccccggggca acagtcggat
      601 atcaggccgg acgctgacct gatttgccgg ttcgcgaaac cgcccggatg ctctaatccc
      661 gcgccatgat caaacgactg accgccgacc tcaagtccgg cgccctgacc gaggccctgg
      721 ccgatatcgc cgaggacgcc gcccgtgtca tcctgcccta ctggcgtagc ggcgtggtgg
      781 cggagaccaa ggctgacgac agccccgtca ctcaggctga ccgggaggcc gaggccctga
      841 tcctgagccg gctggaggcc ctgtatccgg gcgtccagac cgtcgccgag gaggccgtcg
      901 ccgccggggg cgcgccggcc gaggccgccg aatggttctg gctgatcgat ccgctggacg
      961 gcacgcgcgg cttcgtcgag gggcgggaga gctttaccgt caacatcgcc ctgatccaca
     1021 agggcgcgcc ggtggcgggc gtggtgacgg ccccggccac ggccacgacc tggcgcagcg
     1081 gtccggcgaa gggcggcgcc ttccgccgcc gcttcggcga gggctggcag gcgatcaagg
     1141 tgcgcaaccg gcccgcctcc cccatggccg taatgagcca cagcatgacc gacgctgagg
     1201 ccgagcgcct ggcctcacgg cacggttgcg tgcagtggca ggggaccgat tcgtcgctga
     1261 aattctgcct gatcgccgag gggcggttcg acgcctatcc gcgcacgggc ccgacgtctg
     1321 aatgggacac ggcggcgggg caggcggtgc tggaagccgc gggcggacgg gtgatgggcg
     1381 aggacggtca gccgctgcgc tacgggaagt cctgtttcgc caacggcggg ttcgtggcgc
     1441 tgggcggcta ggcgcggcta gaggctgacc ttttccggca tggccagcat ggcccgctcg
     1501 agcagggcgg gcgcccccca ttcgcgggcg gtctccatcg cctcggcgag catcagaccg
     1561 atcgcgcggg gctcgacccc catcagccgc cccttggcca tggccagccg cgccatgccg
     1621 atctggtcgg cggcccagtc gagcggccgc gcggcggggg ccgtcaggcg ctgacgaacg
     1681 gccgtttcga gcccgatcaa tgcggcgagg tcgcccacgt cctcggccag gtcgacctcc
     1741 gtcgccagcc ggcgctcgcg ggccaaggca cccagcacca gacccggttc gcgggtcaag
     1801 gcctcggcct gcgccaaggt aagcagaggc gtttcgccgg gcgtctggac cagctggacg
     1861 gcgacccagt ccagcggact gtgatcggag gtgaactggt cggcggcggc gtcgaacagg
     1921 gcccggccct gctcggccgc cgcgtcgctt cgcgccagcc gggccagggc gataaggccg
     1981 gcgccggcga gggccagcgc gcgggcgcgg gtcagggggc ggtagtcggg cgaggcggcc
     2041 tcgaccagcg cgcgcagatc gcgtccggcc tgatccagca gccggccgtc gcgccg
//
LOCUS       DASVVN010000067         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_2666, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000067 DASVVN010000000
VERSION     DASVVN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VF686_01085"
     CDS             <1..296
                     /locus_tag="VF686_01085"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEX8660429.1"
                     /translation="QIASGANLLAFTTGRGSVFGAKPAPSIKLASNARVASWMDEDID
                     IDASPVLDGVSLAAMGEIIFARMLAVASGEPSRSEALGIGDNEFVPWQVGGYL"
     gene            416..1717
                     /locus_tag="VF686_01090"
     CDS             416..1717
                     /locus_tag="VF686_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660430.1"
                     /translation="MSNVAGGTANQPAPAGKYRWVVVWLLFAAMVINYVDRQAIGVLK
                     PTLMTEFSWTEGDFANIIFWFQAAYAVSYLLWGRIVDRIGARWGFGIAFVIWQIAFIA
                     HAGVRSLNQMIFARLALGVGEGGGFPAGIKAVTEWFPKKERAFAVGLFNAGTNIGAIV
                     TPLIIPLIASVWLGTAEDPNWRMALIIVGAAGLIWVPIWFMVYKRPGEHKKVTAEELD
                     YIAQDAPDPAHKIGWLKLLTVRETWAFALGKFLIDPIWWFFLFWLPGFLGAQYDLNLL
                     TFGPPLIAIYLLSDVGSIGGGWLSSQFLKRGMSLNAARKLTMLICAIAVIPVAFAGMA
                     SNVWVAVLIIGIATAAHQGFSANLYAMPGDVFPRSAVGSVVGIGGMIGGFGGMAMAKY
                     AGYVLDKIGSYTPIFVVAASAYLIALLVIHLLSPRYEPAKV"
BASE COUNT          290 a          643 c          606 g          337 t
ORIGIN      
        1 gccagatcgc ctcgggcgcc aatctgctgg ccttcaccac cggccgcggg tcagtgttcg
       61 gcgccaagcc ggcgccgtcg atcaagctcg cctccaacgc ccgcgtggcg agctggatgg
      121 acgaggacat cgacatcgac gcctcgccgg tgctggacgg cgtgtcactg gccgccatgg
      181 gcgagatcat ctttgcccgc atgctggccg tggcctcggg cgaaccgtcc aggtcggaag
      241 cgctcgggat cggcgacaat gagttcgtcc cctggcaggt cggcggatac ctctgagcca
      301 tcgcgcgaac aggcgttgac accggttacc atgcgccccc attctggggc ctgaagtcgc
      361 cgacagccgc ccgggaacag ggcgaatcgg ccgggaaaca cgggacgagg cgatcatgag
      421 caacgttgca ggcggcactg cgaaccagcc ggcgcccgcg ggcaagtacc gttgggtggt
      481 ggtgtggctg ctgttcgccg ccatggtcat caactacgtc gaccgtcagg ccatcggggt
      541 gctcaagccc accctgatga ccgagttcag ctggaccgag ggcgacttcg ccaacatcat
      601 cttctggttc caggcggcat acgccgtgtc ctatctgctc tggggccgga tcgtggacag
      661 gatcggggcc cgctggggct tcggcatcgc cttcgtcatc tggcagatcg ccttcatcgc
      721 ccacgcgggc gtccgcagcc tgaaccagat gatctttgca cgactggccc tgggcgtcgg
      781 cgagggcggc ggcttcccgg ccggcatcaa ggcggtgaca gagtggttcc ccaagaagga
      841 acgcgccttc gcggtcggcc tgttcaacgc cgggacaaat atcggggcca tcgtcacccc
      901 gctgatcatt ccgctgatcg cctcggtctg gctgggcacg gccgaggatc cgaactggcg
      961 gatggccctg atcatcgtcg gcgcggccgg tctgatctgg gtgccgatct ggttcatggt
     1021 ctacaagcgc ccgggcgagc acaagaaggt caccgccgag gaactggact acatcgcgca
     1081 ggacgccccc gacccggcgc acaagatcgg ctggctgaag ctgctgacag tcagggagac
     1141 ctgggccttc gccctcggca aattcctgat cgatccgatc tggtggttct tcctgttctg
     1201 gctgccgggc ttcctcggcg cccagtacga cctcaacctg ctgaccttcg gccccccgct
     1261 catcgccatc tatctgctga gcgacgtcgg ttcgatcggc ggggggtggc tgtccagcca
     1321 gttcctcaag cgcggcatga gcctgaacgc ggcgcgaaag ctgacgatgc tgatctgcgc
     1381 catcgccgtc attccggtgg ccttcgccgg catggcgtcc aatgtctggg tcgcggtcct
     1441 gatcatcggc atcgccacgg cggcccacca gggcttctcg gccaacctct acgccatgcc
     1501 gggagacgtc ttcccgcgct cggcggtggg ctcggtcgtc ggtatcggcg gcatgatcgg
     1561 cggcttcggc ggcatggcca tggccaaata cgccggctac gtcctcgaca agatcggcag
     1621 ctacacgccc atcttcgtgg tcgcggcctc ggcctatctg atcgccctgc tggtcatcca
     1681 cctgctgtcg ccgcgctacg aaccggccaa ggtctgatcc cgtccctgag gtcgtccgcc
     1741 ccgcggcgtg acgacctcag acggcggcct tccgtcacgt aaccctatga ataaccacgt
     1801 gcccgacgcg ggcgctgcgt tgtaggctgg tcgcagtccc atccggagcg cgcccatgcc
     1861 gtccctgaag ccctcg
//
LOCUS       DASVVN010000068         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_344654,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000068 DASVVN010000000
VERSION     DASVVN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344654"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VF686_01095"
     CDS             <1..832
                     /locus_tag="VF686_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8660431.1"
                     /translation="AGFLNFLRTDPQFYATTREDLLEKASEMAKRADDGLPALFGTLP
                     RLPYGVRPVPREIEANYTTGRYNQGSIENGIAGGYMVNTSSLDQRGLYELPALTLHEA
                     VPGHHLQIALQQEAEDGPYFRRSVDVTAFIEGWGLYSEFLGEEMNFYRTPYERFGRLS
                     YEMWRACRLVSDTGIHWLGWNLEQARACFRDNSALAPLNIETELQRYIGWPGQATAYK
                     IGEIRLRGVRSRAERELGGRFDVRRFHDALLVDGPLPMALLDERMDRWIAEEKARPAD
                     "
     gene            complement(829..1173)
                     /locus_tag="VF686_01100"
     CDS             complement(829..1173)
                     /locus_tag="VF686_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8660432.1"
                     /translation="MDREGVGKVCLALPGATLDHPFGDHHDAYRVGGKMFVMVGEQGG
                     VSFKVSDIAYEVLTESGHARPAPYLARNKWVNLPRIDDWPDDELAEHMAIAHGIVAAK
                     LTRKVRAELGLT"
     gene            complement(1176..>1647)
                     /gene="msrA"
                     /locus_tag="VF686_01105"
     CDS             complement(1176..>1647)
                     /gene="msrA"
                     /locus_tag="VF686_01105"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8660433.1"
                     /translation="FWTLPGVWVTSVGYSGGITPNPTYEETCTGKTGHAEVVQVVFNP
                     HEISYGELLKAFWENHDPTQGMRQGNDIGTTYRSAIYYLNDEQRAEAEASRDAYQAAL
                     SKAGRGTITTEIAPAGPYYFAEDYHQGYLAKNPAGYCGIGGTGVVCPIGVGVEA"
BASE COUNT          263 a          565 c          539 g          280 t
ORIGIN      
        1 cgcaggcttc ctcaacttcc tgcgcaccga tccgcaattc tacgccacga cgcgcgagga
       61 tctgctggag aaggcttcgg agatggcgaa acgggccgac gacggtctgc cggcgctgtt
      121 cggaaccttg ccccgcctgc cgtatggcgt gcgtccggtg ccgcgcgaga tcgaggccaa
      181 ctacaccacc ggccgctaca atcagggctc gatcgagaac gggatcgccg gcggctatat
      241 ggtcaacacc tcaagcctcg atcagcgcgg cctgtatgag ctgccggccc tgacgctgca
      301 tgaggcggtg cccggccacc acctgcagat cgccctgcag caggaggccg aggacggccc
      361 ctatttccgc cgcagcgtcg acgtcaccgc cttcatcgag ggctggggcc tgtattcgga
      421 gttcctcggc gaggagatga atttctaccg caccccctac gagcggttcg gccggctgag
      481 ctacgagatg tggcgggcgt gccggctggt gtcggacacc ggcatccact ggctgggctg
      541 gaacctcgaa caggctcgcg cctgtttccg ggacaattcg gctctcgcgc cgctgaacat
      601 cgagaccgaa ctgcagcgct atatcggctg gcccggccag gcgacggcct acaagatcgg
      661 cgaaatccgg ctgcgcgggg tgcggtcgcg cgcggagcgc gaactgggcg gccggttcga
      721 cgtccgccgc ttccacgacg ccctgctggt tgacggcccc ctgcccatgg ccctgttgga
      781 cgaacgcatg gaccgctgga tcgccgagga gaaggcgcgg ccggccgact aggtcaggcc
      841 cagctccgcc cggaccttcc tcgtcagctt cgccgccacg atgccgtggg cgatggccat
      901 atgctcggcc agttcgtcgt cgggccagtc gtcgatgcgc ggcaggttca cccacttgtt
      961 ccgcgccaga tagggcgcgg ggcgggcgtg gccggattcg gtcagcacct cataggcgat
     1021 gtccgagacc ttgaacgaga ccccaccctg ttcgccgacc atgacaaaca tcttgccgcc
     1081 gacgcggtag gcgtcgtgat ggtcgccgaa cggatggtcc agcgtcgcgc ccgggagggc
     1141 gaggcagacc ttgccgaccc cctcccgatc catgatcagg cctccacgcc cacgccgatc
     1201 gggcagacca cccccgtacc gccgatgccg cagtagcccg ccggattctt ggccagatag
     1261 ccctggtgat agtcctctgc gaaataatag ggaccggccg gcgcgatctc ggtggtgatc
     1321 gtcccccgcc cggccttcga cagggccgcc tgataggcgt cgcgcgacgc ctcggcctcg
     1381 gcccgctgtt cgtcgttcag ataatagatg gccgaacggt aggtggtgcc gatgtcattg
     1441 ccctggcgca tgccctgggt cgggtcgtgg ttctcccaga aggccttcag cagttcgccg
     1501 tagctgatct cgtgcgggtt gaagaccacc tgcaccacct cggcgtggcc cgtctttccg
     1561 gtgcaggttt cctcataggt cggattgggc gtgatcccgc ccgaatagcc gaccgaggtg
     1621 acccagacgc ccggcagcgt ccagaag
//
LOCUS       DASVVN010000069         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_502548,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000069 DASVVN010000000
VERSION     DASVVN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502548"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..724)
                     /locus_tag="VF686_01110"
     CDS             complement(65..724)
                     /locus_tag="VF686_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660434.1"
                     /translation="MTPSDLEQPVILSLLGAGFVTAFLHAALPTHWLPFVLVGRAQRW
                     SLPRMMGAVTAAGLAHIVTTAIVGGLIVAAGLAMDQWVSGLLPHLSAVLLFLFGAFYL
                     SRSLIRRPVPADGPPLDLAEPAVSHAAAFWGLVVMMAVSPGEVLLPIYLSQAGEGLAV
                     LAALTVTFAVGTVAGMAVFTLLARAGWSVLRLERWARYEGAVLGGALIVIGLLVVAHQ
                     H"
     gene            914..1462
                     /gene="rplU"
                     /locus_tag="VF686_01115"
     CDS             914..1462
                     /gene="rplU"
                     /locus_tag="VF686_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX8660435.1"
                     /translation="MYAVIKTGGKQYRVQPGDVIVVEKLDGDVGSDVSFGDVLMLGGD
                     KGVTLGAPLIAGAAVAATLVETRKGEKIKIFKKTRRQGYRRTNGHRQMETVLRITGID
                     GAGEKAKWDGETSLMTKAEMNLRARGLAPRVEETEAKPVKAPKAAAAPKAEAAAEKPA
                     AAKKAPAKKAAPKAKPASTDEA"
     gene            1490..1771
                     /gene="rpmA"
                     /locus_tag="VF686_01120"
     CDS             1490..1771
                     /gene="rpmA"
                     /locus_tag="VF686_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX8660436.1"
                     /translation="MAHKKSGGSSRNGRDSHSKRLGVKKFGGENVLAGNILVRQRGTT
                     FHPGDNVGLGRDHTLFATAHGAVKFTKKANGRCYVSILPVTADMPMAAE"
     gene            1937..>2460
                     /locus_tag="VF686_01125"
     CDS             1937..>2460
                     /locus_tag="VF686_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660437.1"
                     /translation="MCVIEPSPVVETRRLALRAPGAQDISRLATLANDPDVARMTLRM
                     PHPFGIADAEEFVLRVAVQDPARARTFLIEHEDHGPVGVIGLFEDADPAPEVGYWIAR
                     PFWGRGYATEALQAATVWASKSWKRRALMAGHFSDNPASGRVLEKAGFLYTGEVRKNF
                     SRARNAETQTRRMV"
BASE COUNT          444 a          838 c          824 g          354 t
ORIGIN      
        1 catgggaatg gccatgatcg tggccgtggc cgtggtcgtg atgcgcgtgc gccatgcgcc
       61 agtcctagtg ctgatgcgcc acgacgagaa ggccgatcac gatcagcgct ccgcccagca
      121 ccgcgccttc gtagcgcgcc cagcgctcca gccgcagcac cgaccagccg gcgcgggcca
      181 gcagggtgaa gacagccatg ccggcgacgg tgcccaccgc gaaggtcacg gtcagggccg
      241 ccagtacggc caggccttcg cctgcctgcg acagatagat gggcagcagc acctcgcccg
      301 gcgaaaccgc catcatcacg accagacccc agaaggcggc tgcgtgggac acggcgggct
      361 cggccaggtc cagcggcggc ccgtcggcgg gcacgggacg acgaatgagg gaccggctca
      421 gatagaaggc gccgaagagg aacagaagga ccgccgacag gtggggcagc aggcccgaca
      481 cccactggtc catggccagg cccgcggcga cgatcaggcc gccgacaatg gccgtggtga
      541 cgatgtgcgc cagacccgcc gccgtcaccg cgcccatcat ccggggcagg ctccagcgct
      601 gcgcccgtcc gaccagcacg aacggcagcc agtgcgtggg cagggcggcg tgcaggaagg
      661 ccgtcacgaa gcccgcgccc agaagggaca ggatcacggg ctgttcgagg tcggatggcg
      721 tcatcgccgc ctccatagcc tgttacttta taacagtcga gaggggtttc cggttttcag
      781 cgacggcggc gaacccccgt tttttcgccc gtccgcgttg actcgcacac ccctctgcgt
      841 ctatgtcgcc gctcttcgcc cgagggggtc ccccgtcggg cttattcttt ttgcgtttcg
      901 ctgaggcttc aacatgtacg cggtgatcaa gaccggcggc aaacagtacc gggttcagcc
      961 gggcgacgtc atcgttgtcg agaagctcga cggcgatgtc ggttccgacg tttcgttcgg
     1021 cgacgtgctg atgctgggcg gcgacaaggg cgtgaccctc ggcgctcccc tgattgcggg
     1081 cgccgctgtc gccgcgaccc tggtcgagac ccgcaagggc gagaagatca agatcttcaa
     1141 gaaaacccgt cgtcagggct accgccgcac gaacggccat cgccagatgg aaaccgtgct
     1201 gcgtatcacc ggcatcgacg gcgccggcga aaaggccaag tgggacggcg aaacctcgct
     1261 gatgaccaag gccgagatga atctgcgcgc ccggggcctc gccccgcgtg tcgaggaaac
     1321 cgaagccaag ccggtgaagg ctcccaaggc cgccgccgct ccgaaggccg aagccgccgc
     1381 tgaaaagccg gccgccgcca agaaggctcc ggccaaaaag gctgcgccca aggccaagcc
     1441 cgcctccacc gacgaagcgt aagaacagtt agagacaggg agcagaccta tggctcacaa
     1501 gaaatccggt ggttcgtcgc gtaacggtcg cgactctcac tccaagcgcc tcggcgtgaa
     1561 gaagttcggc ggcgagaacg ttctggccgg caacatcctc gtgcgccagc gcggcaccac
     1621 cttccacccg ggcgacaacg tcggcctggg ccgcgaccac accctgttcg cgaccgccca
     1681 cggcgccgtc aaattcacca agaaggcgaa cggccgttgt tacgtgtcga ttcttccggt
     1741 caccgccgac atgccgatgg ccgccgagta gcgcaaaccg actgacaccc ggatcgcgca
     1801 aggctcgaga gccgcgatcc ggaccaggga aatatttccg cggggagtct ggatcaccag
     1861 gctccccgtt ttcgtttccc cgcctcgacg ccgcccgagc cctccaagga ggcggcagtt
     1921 cgaacgaggc cgtgtcatgt gcgtgattga accctctccc gtcgtcgaga cgcggcgcct
     1981 ggcgctgcgc gcgcccggag cccaggacat cagccgtctg gcgactctcg ccaacgatcc
     2041 ggacgtggcg cgcatgaccc tgcgcatgcc gcaccccttc ggcatcgccg acgccgagga
     2101 attcgtgctg cgcgtggcgg tgcaggatcc ggcgcgcgcg cgcaccttcc tgatcgagca
     2161 cgaggaccat ggtcccgtcg gggtgatcgg cctgttcgag gacgccgatc ccgcgcccga
     2221 ggtcggctac tggatcgcgc ggccgttctg gggccgcggc tatgcgaccg aggcgctgca
     2281 ggcggcgacg gtctgggcgt cgaagtcgtg gaagcggcgg gcgttgatgg ccggacattt
     2341 ctcggacaac cctgcgtctg gccgggtgct ggagaaggcg ggcttcctct acacgggcga
     2401 ggtccgcaag aacttcagtc gtgcgcggaa cgccgagacc cagacgcgcc ggatggtttg
//
LOCUS       DASVVN010000070         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_2721, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000070 DASVVN010000000
VERSION     DASVVN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF686_01130"
     CDS             complement(<1..448)
                     /locus_tag="VF686_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660438.1"
                     /translation="MTDAPAAVAPARRSNGILAAFSGPCLPLAAFGVALPVTLPEFYA
                     TYVGLELGVVAAVFMAVRLIDIVFDPFIGWGMDKTKTKWGRYRPWMAISTPILMLSAL
                     MMFVLVQPGAGAAYLFGWLLVLYLGFSIGTLGQLGWASVLAPQYDQR"
     gene            559..1443
                     /locus_tag="VF686_01135"
     CDS             559..1443
                     /locus_tag="VF686_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8660439.1"
                     /translation="MLMRLILLLLTAAGLFAATATEAQTRVRSGQAEVCDADAIEWAG
                     PAFANSVSWQRMEWTPFGPTEYGWETYLPLIQHELGTDCAPNSPVFARKMAEFQIGHG
                     LPPTGWFDAATFQTFRGLWQERRPFIMARVREDPCPDPPPLYQLGYLVESEEHADRLT
                     RLLRRDVLDAYRQMVAAARAEVPEVANDPELLQIFSGFRDPEADAARCAASGNCDGLR
                     RAVCSPHRTGTAVDLYVGQLAGLGVDNTSPSSRLHMTRGPTYRWLVKNAGRFGFTPYA
                     YEPWHWEWISPTGGYAGQ"
     gene            1440..1904
                     /locus_tag="VF686_01140"
     CDS             1440..1904
                     /locus_tag="VF686_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660440.1"
                     /translation="MIRALTAGERALAGEAFADSLTLETIRFLKSPPPFERAFVAGRW
                     FGRDWIVWPGATLPEDIASAPLRTQATFIHELTHVWQAQQGVNLLTGKLRAGDRPASY
                     EYPVGVDRGWADLNIEQQAMVVEHRCRLSRGERTPGDQAFYDRVCPIGRSSA"
     gene            complement(1894..2271)
                     /locus_tag="VF686_01145"
     CDS             complement(1894..2271)
                     /locus_tag="VF686_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8660441.1"
                     /translation="MFDHLSLGVRDLGAARRFYAAFLGPLGHGETTATEAELAYGPGG
                     ETSQFFLYPVSGDRISGLGTHIAFTAHNPAAVDAACAAAIANGATVVRAAGKHPDIAP
                     DYYGAVLLDPDGNKLEIVAGLMH"
BASE COUNT          404 a          836 c          826 g          387 t
ORIGIN      
        1 tgcgctggtc gtattgcggg gccaggaccg aggcccagcc gagctggcca agcgtgccga
       61 tcgagaagcc gaggtagagg accagcagcc agccgaacag ataggcggcg ccggctccgg
      121 gctggaccag cacgaacatc atcagggccg acagcatcag gatcggcgtc gagatcgcca
      181 tccacgggcg atagcggccc catttcgtct tcgtcttgtc catgccccag ccgatgaagg
      241 ggtcgaagac gatgtcgatc agccgcaccg ccatgaagac ggcggccacc acgcccaact
      301 ccagccccac ataggtggcg tagaattcgg gcagggtgac cggcagggcc acgccgaagg
      361 ccgccagcgg caggcagggc cccgagaagg cggcgaggat cccgttggaa cggcgggcgg
      421 gggctacggc ggcgggtgcg tcggtcatgg acaggtccgg cgatccggcc cgagtcggcc
      481 gtggcctcac catgccgact ttgcgggcat ccgcgaaggg ccttgccgtg gccacacagt
      541 cagggtcacc atcgccccat gctgatgcgt ctgatcctgt tgctgctgac cgccgccggc
      601 ctgttcgcgg cgacggcgac cgaggcgcag actcgcgtgc ggtcgggcca ggccgaagtc
      661 tgcgacgccg acgctatcga atgggccggt cccgccttcg ccaacagcgt gtcctggcag
      721 cggatggaat ggacgccctt cggtccgacc gaatacggct gggagaccta tctgccgctc
      781 atccagcatg aactgggcac ggattgcgcg cccaactcgc cggtgttcgc gcgcaagatg
      841 gccgagtttc agatcggcca cggcctgccg cccaccggct ggttcgacgc cgcgaccttc
      901 cagaccttcc gcggcctgtg gcaggagcgt cgccccttca tcatggcccg ggtgcgcgag
      961 gacccatgcc ccgatccgcc gcccctctac cagctcggct atctggtcga atccgaagag
     1021 cacgccgacc gtctgactcg cctgctgcgg cgcgacgtgc tggacgccta ccggcaaatg
     1081 gtcgccgccg cgcgcgccga ggtgcccgag gtcgccaatg atccggagct gctgcagatc
     1141 ttctccggct ttcgcgaccc cgaggccgac gcggcccgct gcgccgcctc gggcaactgc
     1201 gacgggctga ggcgggcggt gtgttcgccg caccgtaccg gaacagcggt ggacctttac
     1261 gtcggccagc tggcgggact gggggtcgac aacacctcgc cgtccagccg cctccatatg
     1321 acccgcggcc cgacctatcg ctggctggtc aagaacgccg gccggttcgg cttcaccccc
     1381 tatgcctatg agccttggca ctgggaatgg atcagcccga ccggcggcta cgccggacaa
     1441 tgatccgtgc gctgacggcc ggagagcggg cgctggccgg ggaggcgttc gccgattccc
     1501 tgaccctcga gacgatccga ttcctcaagt cgccgccgcc gttcgaacgg gccttcgtcg
     1561 ccggtcgctg gttcggacgg gactggatcg tctggccggg cgcgaccttg ccggaggata
     1621 tcgcctcggc gccgctcaga acccaggcca ccttcatcca tgaactgacc catgtctggc
     1681 aggcgcagca gggggtgaat cttctgaccg gcaagctgag ggccggggac cgtcccgcat
     1741 cctacgaata tccggtcggc gtggaccgcg gctgggccga cctgaacatc gagcagcagg
     1801 ccatggtggt cgaacaccgg tgccgcctgt cgcggggcga gcgaacgccg ggcgatcagg
     1861 ccttctatga tcgggtctgc ccgatcggcc ggtctagtgc atgaggccgg cgacgatctc
     1921 cagcttgttg ccgtcgggat ccagcagaac ggcgccgtag tagtcgggcg cgatgtccgg
     1981 atgttttccg gcggcgcgca ccacggtcgc gccgttggcg atggcggcgg cgcaggccgc
     2041 gtctacggcc gcgggattgt gtgcggtgaa ggcgatgtgg gttcccagac ccgaaatgcg
     2101 gtcgccgctg accggataca ggaaaaactg actggtttcg ccgcccgggc cataggccag
     2161 ctcagcctcg gtcgccgtgg tctcgccatg gcccagcggc ccgaggaagg cggcgtagaa
     2221 ccgacgggcc gcgccaagat cgcgaacgcc caaagacaaa tgatcgaaca tgcttccatc
     2281 tcccggccaa gctttccgca tctggggaag gacgccgcca cgcgcaagtt tctgaggccc
     2341 gtcacttgcg cgggatcgca gaccatccaa tttaagcaca gttactgtta ctgaatatcc
     2401 gcctcccgga aaggtccctt gtcctccatg cgccagccag ctgtcttctt cgg
//
LOCUS       DASVVN010000071         5656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_291841,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000071 DASVVN010000000
VERSION     DASVVN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291841"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1324)
                     /gene="recA"
                     /locus_tag="VF686_01150"
     CDS             complement(242..1324)
                     /gene="recA"
                     /locus_tag="VF686_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX8660442.1"
                     /translation="MAAQANLKLVGKDDGDKQRALEAAIAQIDRAFGKGSVMKLGKNG
                     VTEAIPSVSTGSLGLDMALGIGGLPRGRVIEVFGPESSGKTTLALHTVAEIQKAGGTA
                     AFVDAEHALDPGYAQKLGVNLDDLLVSQPDTGEQALEIVDTLVRSGAVDIVVVDSVAA
                     LTPRAEIEGEMGDSLPGLQARLMSQALRKLTASINKSQCIVLFINQIRHKIGVMYGSP
                     ETTTGGNALKFYASVRLDIRRTGAIKDREEVVGNTTRVKVVKNKVAPPFREVIFDIMY
                     GEGISKLGEIIDLGVKAGIVEKSGSWFSYDSQRIGQGRENVRAFLKANPDVADSIEKA
                     VRASTSKIAEELLGTPEPDEGQDLEG"
     gene            1563..2855
                     /locus_tag="VF686_01155"
     CDS             1563..2855
                     /locus_tag="VF686_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8660443.1"
                     /translation="MILRVFLSLFAVASFAAAPDAGQTLDPQAQREVVSHLNVALQEN
                     YIFPDRIPAISAELDRRVQSEPIDRQRFAESLTQGLIRASEDLHFSVAFDPDGVAADR
                     RAKAEGETTTQAQHDAEQAANFGFRDVRRLDGGLAYVRFDFFADPQYAQETAAGVMRF
                     AEGAKGLILDLRYNNGGVLEMAQFLMSYLYPAGKEQEFFDYNYNDHGVRVERSQWNLA
                     AVPGQRLADIPVVVLTGSTSFSSAEWMAFSLQRLGRATVIGERTAGGAHPVTRVPIDD
                     RFMLQVPFGQIRDPVKGQDFEGVGVTPDLVVPASEALLAARKFLLQGRAEAGDADARW
                     ALIPIEFAISGEIPSGTDLDDAAGTYEGRILRRTATGLTYHWRDRFVLALDPIGKDLF
                     AVQGTDDYRLRLVRENGDVSGLERVWKSGESVTYRRLD"
     gene            2871..3326
                     /locus_tag="VF686_01160"
     CDS             2871..3326
                     /locus_tag="VF686_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8660444.1"
                     /translation="MIELDRTFLGRLSEQLSELIEQQSAEVFKRAGIVIPVKSSSLLG
                     AIATLGPVSVADLVRALDRSHQLIQQKMPKLLTLGLVSRRPNPDDQRIILIEITDRGR
                     EQLALLDGLKPEFDRAYAEMEKETGPVFEGLNHAISALKSRPLIGRMAV"
     gene            complement(3358..3804)
                     /locus_tag="VF686_01165"
     CDS             complement(3358..3804)
                     /locus_tag="VF686_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8660445.1"
                     /translation="MSTPLWLEDLHVGQTFVSGPAALTAEDIKTFARQFDPQPFHLDE
                     AAAKDSLFGGLAASGWHTAALTMRLLVEAFPLAGGLVGAGGETTWPRPTRPGDTLTVH
                     VEVLEILPSRTRPDRGMIRTRTETRNQHGDAVQISTTRIVAWRRPA"
     gene            complement(3801..>5656)
                     /locus_tag="VF686_01170"
     CDS             complement(3801..>5656)
                     /locus_tag="VF686_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8660446.1"
                     /translation="ALALLIAVLTLQSPRPVPASAPATDFSAQRAMVDVREIAQRPHP
                     VGSADHLRVRTLLLERMTALGLQTSTQTGPLSPAAVQRIERNGGSAAGLELTNLIGVL
                     PGRDPRLPAVALMAHYDTVPESAGAADDTSGVAAILETVRALRARGPADRTLVVLLTD
                     GEELNLDGARVFWGGHPLRDRIGAAVNLEARGGGGRAMMFETGRGNARTIGLFARAGV
                     RATGGVSSNSLAVFVYELMPNGTDFTIPKDRGVQGINLAFIGRPDQYHAGASTPDALD
                     QGSVQHIGSQALETSDALLRATALPAATQNVVYADLFGRVIVGHAPATGWLLLGLAFL
                     GLGAAMWRARSRAGLTFADVGRGMVDGLWFLSTGLVLTQAVRLLAGPLSSRAESADAY
                     YTLLARLPWMEAGTVLTVLALALALMGGRTRPDPRVVAGGIAAAALLALGLGGVNVIV
                     IGAAVVAIGLSLFPALAARSVWGGWSGLIGLILVLAAAAQAAAPEAAFLFVWTGLLAA
                     LTAAVAAFLDPGLTRPKSLAPIAVAAVLGAGWIGGLAHTVFLGIGMDLPGVLVLLGLL
                     VLMFVRPLSPERAVARPLLMAAMAILLLGAALSGAARFAEPQPAPDTTAAA"
BASE COUNT          905 a         1826 c         2007 g          918 t
ORIGIN      
        1 gcctggccgt tatcacaaga aaaaaggccg cccgacccgg gtggggatcg agcggccgct
       61 ccgtccggcc cggcccggcg ggggtcggat ggcgggtcgg cggacccggc cggagaagtt
      121 gattttgcga tggggaagag accggccttc gaaaggcgcg gacattccgg tcacatcgga
      181 ttctggcgtg agatgaagag tccggtctgc agcattccgg actcttcggt cacatcggac
      241 tctagccttc gaggtcctga ccttcgtcgg gttcgggggt gcccagcagt tcctcggcga
      301 tcttggaggt cgaggcgcgc acggcctttt cgatcgagtc ggcgacgtcc ggattggcct
      361 tgaggaaggc gcgcacattc tcgcggccct ggccgatgcg ctggctgtca taggagaacc
      421 agctgcccga cttctcgacg atcccggcct tgacgcccag atcgatgatc tcgcccagct
      481 tggagatgcc ctcgccgtac atgatgtcga agatgacctc gcggaacggc ggggcgacct
      541 tgttcttgac caccttgacc cgggtggtgt tgccgacgac ctcttcgcgg tccttgatcg
      601 cgccggtgcg gcggatgtcg agacggaccg aggcgtagaa tttcagcgca ttgccgcccg
      661 tcgtcgtttc cggcgagccg tacatgacgc cgatcttgtg gcggatctgg ttgatgaaca
      721 ggacaatgca ctgcgacttg ttgatcgagg cggtcagctt gcgcagggcc tggctcatca
      781 ggcgggcctg aaggccgggc aggctgtcgc ccatctcgcc ctcgatctcg gcgcgcgggg
      841 tcagggcggc gacggagtcg accacaacga tgtccacggc gcccgagcgc accagggtgt
      901 cgacgatttc cagcgcctgt tcgcccgtgt cgggctggga caccagcagg tcgtcgagat
      961 tgacccccag cttctgggcg tagcccgggt cgagtgcgtg ttcggcgtcg acgaaggcgg
     1021 cggtgccgcc ggccttctgg atctcggcca cggtgtgcag ggcgagcgtc gtcttgcccg
     1081 agctttcagg cccgaagacc tcgataaccc ggccacgcgg caggccgccg atgcccagcg
     1141 ccatgtcgag gcccagcgag cccgtcgaga ccgagggaat ggcctcggtc acgccgttct
     1201 tgcccagctt catcaccgag cccttgccga aggcccggtc gatctgcgcg atcgccgctt
     1261 ccagcgcgcg ctgtttgtcg ccgtcgtcct tgcccaccaa cttcaaattc gcctgagctg
     1321 ccaccttgcc actctccctt gccatgattc ccatctggtt ccgggagaga cccgcctgga
     1381 cccgctccct gttgagacgc agttagtaca cggtttgttc cggttgccaa tatgttcttt
     1441 tcggaccgcc gcggggatgc gaccaaattt gacgtatcga tgctcaggca agccgttccg
     1501 gggcattgac ctgacgttga ttacaacata ttgcgcaata tgttgtatat ctggagccga
     1561 agatgatcct gcgcgttttt ctgtcgctgt tcgctgtcgc cagcttcgct gcggcgccgg
     1621 atgccggcca gaccctggat ccgcaagcgc agcgggaggt ggtttctcac ctcaacgtgg
     1681 cgctacagga gaattacatc tttccagacc gcatccccgc catatccgcc gagttggaca
     1741 gacgcgttca gtcagagccg atcgatcgcc agcgtttcgc cgagagtctc acgcaggggc
     1801 tgatcagggc gagtgaggat ctgcatttct cggtggcctt cgaccctgat ggcgttgccg
     1861 ctgaccgccg ggcgaaggcg gagggcgaga cgaccaccca ggcgcagcac gatgcggagc
     1921 aggcggccaa tttcggcttt cgtgacgttc gcaggctcga cggcggtctg gcctatgtcc
     1981 ggttcgattt cttcgccgat ccgcaatatg cccaggagac ggcggcaggg gtcatgcggt
     2041 ttgccgaagg cgccaagggg ctcatccttg accttcgcta caacaacggc ggcgttctgg
     2101 agatggcgca attcctgatg agctacctct accccgccgg caaggagcag gagttcttcg
     2161 actacaacta taatgaccac ggggttcgcg tggagcgaag ccaatggaat ctggccgccg
     2221 tacccggcca gcgtttggcg gatattcccg tcgtcgtgct gaccggatca acgtcgttct
     2281 cgtctgccga atggatggcg ttctcgctgc agcggctcgg gcgcgcgact gtgatcggcg
     2341 aacgcaccgc cggcggcgcg catcccgtca cccgcgtgcc gatagacgac cgcttcatgc
     2401 tgcaggtgcc gttcggtcag atccgggatc cggtgaaggg gcaggatttc gagggcgtcg
     2461 gcgtgactcc cgaccttgtc gtcccggctt ccgaagccct gctggccgcc cggaaattct
     2521 tgctgcaggg ccgggcggag gcgggcgacg cagatgcgcg atgggcgctg attcccatcg
     2581 agttcgcgat ctcgggcgaa atccccagcg ggacagacct ggatgatgcg gccggaacgt
     2641 acgaggggcg aattcttcgt cgcaccgcga ccggtctgac ctaccactgg cgcgatcgct
     2701 tcgttctggc gcttgacccg atcgggaagg atctgtttgc ggtccaggga acggatgact
     2761 accgcttacg ccttgtccgt gaaaatggtg acgtgagcgg cctggagcgc gtgtggaaat
     2821 ccggcgagtc agtcacctac cgtcgtctgg attgaggggc cgctggagag atgattgagt
     2881 tggaccgcac cttcctgggt cgccttagcg agcagttgag cgaactgatc gagcaacagt
     2941 ctgcggaagt tttcaagcgc gccggcattg tcattcccgt caagtcgtcg tccttgctgg
     3001 gcgccatcgc gacgctcggg cctgtctctg tcgccgatct cgtgcgggcg ctggaccgtt
     3061 cgcaccaact cattcaacag aagatgccga agctgctgac gctgggcctg gtcagccgcc
     3121 ggccgaatcc ggacgatcag cgcatcatcc tgatcgagat cacggacaga gggcgtgaac
     3181 aactggcgtt gctcgacggt ctgaaacccg agtttgaccg cgcctatgcc gaaatggaga
     3241 aggaaaccgg tcccgtgttc gaaggactga accacgcgat cagcgctctc aagtctcgcc
     3301 ccctgatcgg tcgcatggcc gtctgaagtc cgctacgcgc gctgacggtg gctcacctca
     3361 ggctggacgc cgccaggcca cgatgcgagt ggtcgagatc tggaccgcgt cgccgtgctg
     3421 gttgcgggtc tcggtgcggg tgcggatcat gccgcggtcg ggccgggtcc gggacggcag
     3481 tatctccagc acctcgacat ggacggtgag cgtatccccc gggcgcgtcg ggcgcggcca
     3541 ggtggtctcg ccgcccgcgc cgacgaggcc gcccgcgagc gggaaggcct cgaccagcag
     3601 gcgcatggtc agggccgccg tgtgccagcc gctggcggcc agaccgccga acaggctgtc
     3661 cttcgccgcc gcctcgtcga ggtggaaggg ctgcgggtcg aactgacggg cgaacgtctt
     3721 gatgtcctcg gccgtgaggg cagcgggacc ggacacgaag gtctggccga cgtgaaggtc
     3781 ttcgagccag aggggcgtgc tcatgcggcg gcggtcgtgt ctggcgcagg ctggggctcg
     3841 gcgaaccggg ccgccccgga cagggccgcg cccagcagca ggatcgccat cgccgccatc
     3901 agcaggggcc gcgccaccgc ccgctcgggg ctgagcgggc ggacgaacat cagcaccagc
     3961 aggccgagca ggaccagcac gccgggcagg tccatgccga tgccgaggaa gaccgtgtgg
     4021 gccagaccgc cgatccagcc ggcgcccagc acggcggcga ccgcgatcgg cgccagcgat
     4081 ttcgggcggg tcaggccggg atccaggaag gcggcgacgg cggcggtcag ggcggccagc
     4141 aagccggtcc agacgaacag gaaggcggct tcgggcgcgg cggcctgggc ggccgcggcg
     4201 aggaccagga tcaggccgat cagcccgctc catccgcccc agaccgagcg ggcggcgagg
     4261 gcggggaaca gcgacaggcc gatggccacg accgcggcgc cgatgacgat gacgttcacg
     4321 ccgcccagcc cgagggccag aagggcggcg gccgcgatcc cgccggcgac gacgcggggg
     4381 tcgggccggg tgcgtccgcc catcagggcc agggcgaggg ccagaaccgt cagcacggtt
     4441 ccggcctcca tccacggcag gcgggccagc agggtgtagt aggcgtcggc gctctcggcg
     4501 cggctcgaca gcggcccggc cagcaggcgc acggcctggg tcagaaccag accggtcgac
     4561 aggaaccaca ggccgtcgac catgccgcgt ccgacgtcgg cgaaggtcag gccggcgcgc
     4621 gaccgcgccc gccacatcgc cgcgcccagc ccgaggaagg cgaggcccag caacagccag
     4681 ccggtggccg gcgcgtggcc gacgatcacc cgtccgaaca ggtcggcgta gacaacgttc
     4741 tgcgtcgcgg cgggaagggc tgtcgcgcgc agcagggcgt cggaggtttc cagcgcctgc
     4801 gagccgatgt gctggacgct gccctgatcc agcgcgtccg gggtagaggc gccggcgtgg
     4861 tactggtccg ggcggccgat gaaagcgagg ttgattccct gaacgccgcg gtccttgggg
     4921 atggtgaagt cggtgccgtt gggcatcagc tcatagacga agaccgccag cgagttggac
     4981 gagaccccgc cggtcgcccg aacgcccgcg cgggcgaaca ggccgatggt ccgcgcattg
     5041 ccacgcccgg tctcgaacat catggcccgg ccgccgccgc cgcgcgcctc cagattgacc
     5101 gccgcgccga tgcgatcgcg caggggatgg ccgccccaga agacgcgggc gccgtccagg
     5161 ttcagctcct cgccgtcggt cagcaggacg accagggtgc ggtcggcggg gccgcgggcg
     5221 cgcagggcgc ggacggtctc gagaatggcg gcgacgccag aggtgtcgtc cgccgcgccc
     5281 gccgattccg gcacggtgtc gtagtgggcc atcagggcga cggcgggcag gcgtggatcg
     5341 cgcccgggca ggacgccgat cagattggtg agttcaagcc cggcggcgga cccgccgttg
     5401 cgctcgatcc gctgcacggc cgcgggcgac agggggccgg tctgggtcga agtctgcagg
     5461 cccagggcgg tcatgcgctc cagcagcaag gtccggaccc ggaggtggtc cgccgagccg
     5521 accggatgcg ggcgctgcgc gatctcgcgg acgtcgacca tggccctttg ggcggagaag
     5581 tcggtggcgg gtgcgctggc cgggacgggg cggggacttt gcagggtcag caccgcgatc
     5641 agcagcgcca gcgcca
//
LOCUS       DASVVN010000072         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_344752,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000072 DASVVN010000000
VERSION     DASVVN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344752"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VF686_01175"
     CDS             complement(<1..1568)
                     /locus_tag="VF686_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX8660447.1"
                     /translation="MSLIRVVLALLAAAVVCGGAPSVGAQPVPPAAQDAPDDALRLAR
                     FVEARAGSTDFAALDRFGVAAMRRNDREGLNRLYHVTWTILNQGDFDKASLWNRRLTE
                     AAERQGDERYKDIARLNTLTIRYDQGETAVAAEMRRTAETGEDWFVRAHAVRLAALAL
                     FDEDRIGEGLELLAEAQASIPVNDPFAATAHAGIWEVTGMGLKQLNDVTGAASAFRKF
                     EIDYSNPAYPRPDFDSLYNLTMMAVKVGDADRAAQYFAAHHRLARRAGLTTLSVYDAS
                     LCATVANARNEPRAVLACLEPYGESLGEAAFVAADALPLRAIARARTGQIAGARRDVD
                     ALRRLGAETGIQSEVDHVEAEVLFAEGRHEEAFRLLRAHMLESEIDAARSYSAGIHQV
                     TGNMQQQLAERRTQLETARSNTGLQRAVIGIGVIFLLFAGATLVWQQRQGGRLRLAQR
                     RAEEANRAKSEFLANMSHEIRTPLNGVVSMADTLARRDLQPEEHEMVELIRSSGATLE
                     RLLSDILDSAKIESG"
     gene            1696..1998
                     /locus_tag="VF686_01180"
     CDS             1696..1998
                     /locus_tag="VF686_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX8660448.1"
                     /translation="MSDTDPVVLINVYRCDPTKLDALMDLLTVMVRAQREAEGFVSAT
                     LHRGLNGKVAAVHAVWRTREDWKAMTRLPRVNAAMEPIMAIATFEPHLYEACEVFD"
     gene            complement(1995..2819)
                     /gene="rlmJ"
                     /locus_tag="VF686_01185"
     CDS             complement(1995..2819)
                     /gene="rlmJ"
                     /locus_tag="VF686_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX8660449.1"
                     /translation="MNYRHSFHAGNFADLVKHALLLWLLERRAADGPLTVIDTHAGAG
                     LYDLTGDAARSREAEAGVTRLMAAEGRPPLIEALAAAVARLNPKGGAALYPGSPLLVA
                     QTLRDDDRYVGFELNPPVLDMLTEALEPYPEAAAEGGDGYDQARTWAAEATGPLVLID
                     PPFEKSDDYLRAADTGATIVRADARATVAIWTPLKDLETFDGFLRRLDQAGAQRVLVA
                     EARLRPLTDPMRMNGCAVVILNPPTGAEAAAGEICGWVAETLGEPGAKADVWFTQA"
     gene            2915..3259
                     /locus_tag="VF686_01190"
     CDS             2915..3259
                     /locus_tag="VF686_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660450.1"
                     /translation="MIDHPVLSDRQRTPPRAVAEAARKGLDLREQHGRGGTEVGVRRA
                     EQLMERGLVSDRDIKSMYSYFARHAVDKRARRWGDADKPSTGYIAWMLWGGDDARDWI
                     ENLRRTLREVGV"
BASE COUNT          487 a         1167 c         1097 g          513 t
ORIGIN      
        1 cccgactcga tcttggcgct gtcgaggatg tcggacagca gccgttccag ggtcgcgccg
       61 gacgagcgga tcagctcgac catctcgtgc tcttccggct gcaggtcgcg gcgcgccagg
      121 gtgtcggcca tcgacaccac gccgttcagc ggcgtgcgga tctcatggct catattggcg
      181 aggaattcgc tcttggcccg gttcgcctcc tccgcccgcc gctgggccag acgcagccgc
      241 ccgccctgcc gctgctgcca gaccagggtg gcgccggcga acagaaggaa gatcaccccg
      301 atgccgatga ccgcccgctg cagaccggta ttggaccgcg ccgtctccag ctgcgtccgg
      361 cgctcggcca gctgctgctg catattgccg gtgacctggt ggatgcccgc gctgtagctg
      421 cgcgccgcgt cgatttcgct ctcgagcata tgggcccgga gaaggcggaa ggcttcctcg
      481 tgccggcctt cagcgaacaa aacctcggcc tcgacgtggt cgacctcgga ctggattccc
      541 gtctcggccc cgaggcggcg cagcgcatcc acatcccgcc gggcgccggc gatctggccg
      601 gttcgggccc gggcgatcgc ccgcagcggc agggcgtcgg cggccacgaa ggcggcctcg
      661 cccagcgatt ctccataggg ctccagacag gccagcaccg cgcggggctc gttcctggcg
      721 ttggcgaccg tggcgcacag actggcgtca tagacggaga gcgtcgtcag cccggcccgt
      781 cgggccagtc ggtgatgcgc cgcgaaatac tgggccgcac gatctgcgtc gccaaccttc
      841 accgccatca tggtcagatt gtacaggctg tcgaagtcgg gccgcggata ggccggattg
      901 gaatagtcga tctcgaattt ccggaacgcg gacgccgcgc cggtgacgtc attgagctgt
      961 ttcagcccca tgcccgtcac ttcccagatt ccggcgtggg cggtcgcggc gaagggatcg
     1021 ttcacgggga tgctggcctg agcctcggcc agcaactcca gcccctcccc gatcctgtcc
     1081 tcgtcgaaca gggccagggc cgccagacga acggcgtggg ccctgacgaa ccagtcctcg
     1141 cccgtttccg ccgtgcggcg catctcggcg gcgacggcgg tctcgccctg gtcgtagcgg
     1201 atcgtcaggg tgttcagccg ggcgatgtcc ttgtaccgct cgtccccctg ccgttcggcc
     1261 gcctcggtca gccggcggtt ccacagcgac gccttgtcga aatctccctg gttcaggatg
     1321 gtccaggtca cgtgatagag ccggttcagg ccctcgcggt cgttccgacg catcgcggcc
     1381 acgccgaacc ggtccagcgc cgcgaagtcg gtggagcccg cccgtgcctc gacgaaccgc
     1441 gccagcctca gggcgtcgtc cggagcatcc tgcgccgccg gcggaaccgg ctgggctcca
     1501 acggacggcg ctccgccgca aaccacagcg gcggccagaa gcgcgagaac aacacggata
     1561 agcgacatgg aaattccgac tgtgtcgcct ttcgtcgcac ggcatgtatt gagcaaggtt
     1621 tagcgaacgc cgattcacct tttccggtcg ccgggatagc gagactcacg ccctgcgatc
     1681 agctagacgc ccgctatgtc cgacaccgat cccgtggtcc tgatcaacgt ctatcgttgc
     1741 gatccgacga agctggacgc gctgatggat ctgctgaccg tcatggtccg cgcccagcgc
     1801 gaggccgagg gcttcgtatc cgccactctg catcggggtc tgaacggcaa ggtggcggcc
     1861 gttcacgcgg tttggcggac ccgcgaggac tggaaggcca tgacccgcct gcccagggtc
     1921 aacgccgcca tggagccgat catggccatc gcgacgttcg agccgcacct gtacgaggcg
     1981 tgcgaggtct tcgactaggc ctgggtgaac cagacgtcgg ccttcgcgcc gggctcgccc
     2041 agggtctcgg cgacccagcc gcagatctcg cccgccgcgg cctcggctcc ggtcggcggg
     2101 ttcaggatca ccaccgcgca gccgttcatc cgcatggggt cggtcagcgg ccgcagccgg
     2161 gcctccgcca ccagcacgcg ctgggccccg gcctgatcga gccgacgcag gaagccgtcg
     2221 aacgtctcga gatccttcag cggcgtccag atcgccaccg tggcccgggc gtcggcgcgg
     2281 acgatggtcg cgccggtatc ggccgcccgg agataatcgt ccgacttctc gaacggcgga
     2341 tcgatcagca ccagcggccc tgtcgcctcg gccgcccagg tccgtgcctg atcgtagccg
     2401 tccccgccct cggccgcggc ctccgggtac ggctccagcg cctcggtcag catgtcgagc
     2461 accggcgggt tcaactcgaa gccgacatag cggtcgtcgt ccctcagcgt ctgcgccacc
     2521 agcaggggcg agccgggata gagggccgct ccgcccttcg ggttcagcct cgccacggcg
     2581 gccgcgagcg cctcgatcag gggcgggcgt ccctcggccg ccatcagccg ggtgacgccg
     2641 gcctcagcct ccctcgaccg cgccgcatcg cccgtcaggt catagaggcc cgcgccggcg
     2701 tgggtgtcga tgaccgtcag cgggccgtcg gccgcgcgcc gttcgagcag ccacagcagc
     2761 agggcgtgct tgaccaggtc ggcgaaattt ccggcgtgaa agctgtgacg atagttcatg
     2821 agcgcttccg tcgtccgccc ggctcctgcg gtcaagggcg gacgggaacc gtccgcgtcc
     2881 gcccgtcgtt cggcgacgcg acctggagtg cgaagtgatc gatcatcccg tcctatccga
     2941 ccgtcaacgc accccgccca gggcggtggc cgaggccgcc cgcaagggtc tcgatctgcg
     3001 cgagcagcat ggccggggcg gcaccgaggt cggcgtacgc cgcgccgaac agctgatgga
     3061 gcgagggctg gtcagcgacc gcgacatcaa gtcgatgtac agctatttcg cccgccacgc
     3121 cgtcgacaag cgcgcccgcc gctggggcga cgcagacaag ccctcgaccg gctatatcgc
     3181 ctggatgctg tggggcggcg acgacgcccg cgactggatc gagaatctgc gccggaccct
     3241 gcgcgaggtc ggcgtgtgac cgtc
//
LOCUS       DASVVN010000073         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_450020,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000073 DASVVN010000000
VERSION     DASVVN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450020"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VF686_01195"
     CDS             <1..1641
                     /locus_tag="VF686_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8660451.1"
                     /translation="IPHGDRSGVVIEPWLTDQWYVDADTMAQPAIRAVEQGDTVFEPK
                     SYEKIYFEWLRNIEPWCISRQLWWGHRIPAWYGPNGEIYVAETEEDAREQAMADYDSE
                     VVLTQDGDVLDTWFSSALWPFSTMGWPEKTEDLERFYPTSDLVTAADIIFFWVARMMM
                     MGLHFMDGEVPFKRVIINGLVRDEKGQKMSKSKGNVIDPLGIIDELGADPLRFTMAIL
                     SGTRDIKLSRQRIEGYRNFGTKLWNAARFAQMNEARRVDGFDPATVDQTINRWIRGEL
                     TKAERAVSEAIEGGRFDDAASALYRFVWNVFCDWYLELAKPVFMGSDEVAKAETRALT
                     AWTLDQTLKLMHPVMPFITEELWDKLAGEGAARTEATLIGAAWPDLPDAFIDAGAEAE
                     IGWLVDLVTEIRQLRAEMNVPPSAKPPLAFVAPDAATAERMARHRDLILTLGRAGELT
                     TVEAAPNGAVTFVSGGATAALSLAGIIDVTAERTRLTKEIAAFDSDIGHVNKKLGNPN
                     FVDRAAPEVVDEQRQKLAEAEAGKAKLQAALARLDAVG"
BASE COUNT          304 a          515 c          564 g          262 t
ORIGIN      
        1 atcccgcacg gcgatcgttc gggcgtggtc atcgagccgt ggctgacgga ccagtggtac
       61 gttgacgccg acaccatggc ccagccggcg atcagggcgg tggagcaggg cgatacggtc
      121 ttcgagccga agagctatga gaagatctat ttcgaatggc tgcgcaacat cgagccgtgg
      181 tgcatctcgc gccagctctg gtggggtcac cgcatcccgg cctggtacgg gccgaacggc
      241 gagatctatg tcgccgaaac cgaggaagac gcccgcgaac aggcgatggc cgattatgat
      301 tccgaggtcg tcctgacgca ggacggggac gttctggaca cctggttcag ctcggccctg
      361 tggccgttct cgaccatggg ctggcccgag aagaccgagg atctggagcg gttctacccg
      421 accagcgatc tggtcacggc ggcggacatc atcttcttct gggtcgcccg gatgatgatg
      481 atgggtctgc atttcatgga cggcgaagtc ccgttcaaac gcgtcatcat caacggcctc
      541 gtccgcgacg agaagggcca gaagatgagc aagtccaagg ggaatgtcat cgaccccctc
      601 ggcatcatcg acgagctggg cgccgatccg ctgcgcttca ccatggctat cttgtcgggc
      661 acgcgcgaca tcaagttgtc acgtcaacgt attgaagggt atcgcaactt cggcaccaag
      721 ctgtggaacg ccgcgcgctt cgcccaaatg aatgaggcgc ggcgtgtcga tggcttcgac
      781 ccagccacgg tcgaccagac catcaaccgc tggatccgcg gcgaactgac caaggccgag
      841 cgcgcggtca gcgaagccat cgagggcggc cgtttcgatg acgcggcttc cgccctgtac
      901 cgcttcgtct ggaacgtgtt ctgcgactgg tatctggaac tggccaagcc ggtcttcatg
      961 ggttcggacg aggtggcgaa ggccgagacc cgcgccctga cagcctggac cctcgaccag
     1021 accctgaagc tgatgcaccc cgtgatgccc ttcatcaccg aggagctgtg ggacaagctg
     1081 gccggggagg gcgcggcgcg cacggaagcg acgctgatcg gtgcggcctg gcccgacctg
     1141 ccggacgcct tcatcgacgc gggcgccgag gccgagatcg gctggctggt cgatctggtc
     1201 accgaaatcc gccagctgcg cgccgagatg aacgtgccgc cctcggccaa accgccgctg
     1261 gccttcgtcg cgcccgacgc ggcgacggcg gagcggatgg cgcgacatcg cgacctgatc
     1321 ctgacgctgg gtcgggcggg cgaactgacg acggtggagg cggcgccgaa cggggcggtg
     1381 accttcgtct cgggcggcgc gacggccgcc ctgtcgctgg ccgggatcat cgatgtgacg
     1441 gcggaacgca cccgcctgac caaggaaatc gccgccttcg acagcgacat cggccatgtg
     1501 aacaagaagc tgggcaaccc caatttcgtc gatcgcgccg cgcctgaagt ggtcgacgaa
     1561 cagcgccaga aactggccga ggctgaggcc ggcaaggcga agctgcaggc ggcgctggcg
     1621 cggctggatg cggtgggatg aacac
//
LOCUS       DASVVN010000074         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_108516,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000074 DASVVN010000000
VERSION     DASVVN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108516"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF686_01200"
     CDS             complement(<1..843)
                     /locus_tag="VF686_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX8660452.1"
                     /translation="MAGPGEQGVRIGLSAVVIALRDRKACVLTTSGEDGSTALPFGPF
                     DPARDRTFELALRDFVTAQTGFRLGFVEQLYTFGDLGRASPRATPGPEQHREVSVGYL
                     ALTADAADAPQAEARWTPVLEIFPWEDRRDGAPACTGPLTADLKAWAEADPRRQARVE
                     TLFATTPAHRWNEGQVLDRYELLYEAGLVAEAARDQDRPPLPAAPGRAMASDHRRILA
                     TGLGRLRSKLKYRPVLFDLMPATFTLSELQTAAEAVAGLSLHKQNFRRGVVRTGLVEP
                     TGRLS"
     gene            complement(847..1440)
                     /locus_tag="VF686_01205"
     CDS             complement(847..1440)
                     /locus_tag="VF686_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX8660453.1"
                     /translation="MLSGRRLDLLDPSPFDIEIEDIAHGLARVARWNGQTIGEHAFSV
                     AQHSCVVEEIAAHIQPGLEPKWRLAALLHDASEYVIGDMISPFKAALGVNYKAFEARL
                     EDAIHLRFGLPPKNPVEIKTLIKKADVACAYFEATQLAGFTKKEGLTLFGAPPAGYDL
                     IIDPLPAPVAQQRYLERYRVLAEATGVVPAPDAWHTE"
     gene            complement(1669..>1987)
                     /locus_tag="VF686_01210"
     CDS             complement(1669..>1987)
                     /locus_tag="VF686_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX8660454.1"
                     /translation="GFHIDANDNDGTARVRASSGSGDSVSVNAQNDAAEIRTSAGGEA
                     TRTSWMLSDNRETESGWRMVGFEARGPVGGPLVVATVRSKDTNRGRAFDDARDLVTLN
                     VGE"
BASE COUNT          290 a          582 c          805 g          310 t
ORIGIN      
        1 ggacaggcgg ccggtgggct cgaccagacc ggtgcgtacg acgccgcggc ggaaattctg
       61 cttgtgcaat gagaggccgg cgacggcctc ggcggcggtc tgaagttcgg acagggtgaa
      121 ggtcgcgggc atcaggtcga agaggaccgg gcggtatttc agcttgctgc gcaggcggcc
      181 cagaccggtc gcgaggatgc ggcggtggtc cgaagccatg gcgcggcccg gagcggcggg
      241 caggggcggg cggtcctgat cccgggcggc ttcggcgacc agaccggcct cgtagagcag
      301 ttcgtaacgg tccaggacct ggccctcgtt ccagcggtgg gcgggagtgg tagcgaacag
      361 ggtctccacc cgggcctgcc gacggggatc ggcctcggcc caagccttga ggtcggcggt
      421 cagcgggccg gtgcaggcgg gggcgccgtc gcggcggtcc tcccacggga agatctcgag
      481 gacgggggtc cagcgggcct cggcctgggg ggcgtcggcg gcgtcggcgg tcagggcgag
      541 atagccgacc gagacctcgc gatgctgttc cgggccgggg gtggcgcgag gcgaagcgcg
      601 gccgaggtca ccgaaggtgt agagctgttc gacgaagccg aggcggaagc cggtctgggc
      661 ggtgacgaag tcgcgcaggg ccagttcgaa ggtgcggtcg cgggcggggt cgaacggccc
      721 gaagggcagg gcggtggagc cgtcctcgcc cgatgtggtc aggacgcagg ccttgcggtc
      781 gcggagcgcg atgacgacgg ccgagaggcc gatgcgaacg ccctgttccc cgggacccgc
      841 catcgcctat tccgtgtgcc aggcgtcggg ggcggggacg acgccggtgg cctcggccag
      901 gacgcggtag cgctcgagat agcgctgctg ggccaccggg gcgggcaggg ggtcgatgat
      961 caggtcgtag ccggcaggcg gggcgccgaa gagggtcagg ccctcctttt tggtgaagcc
     1021 cgccagctgg gtggcctcga aataggcgca ggcgacatcg gccttcttga tcagggtctt
     1081 gatctcgacg gggtttttgg gcggcaggcc gaagcggaga tggatggcgt cttccagtcg
     1141 ggcctcgaag gccttgtagt tgacgcccag cgcggccttg aacggcgaga tcatgtcgcc
     1201 gatgacgtat tcggacgcgt cgtggagcag ggcggcgagg cgccatttcg gctcgagccc
     1261 cggctggatg tgggcggcga tctcctcgac gacgcaggag tgctgggcga ccgagaaggc
     1321 gtgttcgccg atcgtctggc cgttccagcg ggcgacgcgg gccaggccgt gggcgatgtc
     1381 ctcgatctcg atgtcgaacg gcgaggggtc gagcaggtcg aggcggcggc ccgacagcat
     1441 gcgctgccag gcgcgaggcg gtttcgcgga tttgcggggc gtggcggcca aggtctgatc
     1501 ctgcacgaga ccatttttgc ttcgggtcga agagacccga atccaaaaaa tggtccagtc
     1561 ttaatatcga gcgaaatctg agccgagttg gactgcgtcc aactcgaccg atttcgctcg
     1621 gggcagggtt ggtgacgcat cggggcgatg cgatcaagac cggagggctt attcgccgac
     1681 gttgagggtg acgagatccc gggcgtcgtc gaaggcgcgg ccgcggttcg tgtccttcga
     1741 gcggacggtg gcgacgacca gcgggccgcc aaccgggccg cgcgcctcga aaccgaccat
     1801 gcgccagccc gattccgttt cccggttgtc gctgagcatc cagctggtgc gggtggcctc
     1861 gccgccggcg ctggtgcgga tctcggcggc gtcgttctgg gcgttgacgc tgacgctgtc
     1921 gccgctgccc gacgaggcgc ggacgcgggc cgtgccgtcg ttgtcgttgg cgtcgatatg
     1981 gaagccg
//
LOCUS       DASVVN010000075         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_213292,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000075 DASVVN010000000
VERSION     DASVVN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213292"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="aroA"
                     /locus_tag="VF686_01215"
     CDS             <1..1171
                     /gene="aroA"
                     /locus_tag="VF686_01215"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX8660455.1"
                     /translation="DDVLATARAVEAFGAKVERLGEGRWRVTGRGGFSRPAGVIDCGN
                     AGTGVRLLMGAAAGYPIGVTFDGDASLRKRPMKRVTGPLAEMGARFGWKAEPDRLPVE
                     VQGGSLSAIDFLQPVASAQVKSAILLAGLNADGVTTVVEPEPSRDHTERMLRAFGAEV
                     GVLPVEAGWRVSLKGGQVLIGTQVAVPGDPSSAAFPLAAGLIVPGSEVTVEGVMLNPL
                     RTGLFETWREMGADLTVSNRRMAGGEEVGDVTARHSPLTGVVVPPERAASMIDEYPIL
                     AATAAFATGSTVMRGIGEMRVKESDRIALMAAGLKACGVAVEEEAEGFTVTGGPVRGG
                     ATVTTHGDHRIAMSHLVLGLAADEAVTVDEPGMIATSFPGFMELMQRLGARIEAG"
     gene            1171..1473
                     /locus_tag="VF686_01220"
     CDS             1171..1473
                     /locus_tag="VF686_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660456.1"
                     /translation="MRWPNPPQPPTTRRPKSKGRLVFDAALFTGTALVMGWAGLDRAQ
                     AGRVTLRSWPDVLVTDQPGLFWALIAGHWVIVAVCLGLSGLAIRALLAPSAKDVRP"
     gene            1470..2114
                     /gene="cmk"
                     /locus_tag="VF686_01225"
     CDS             1470..2114
                     /gene="cmk"
                     /locus_tag="VF686_01225"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX8660457.1"
                     /translation="MNLVIAVDGPAASGKGTIAARLAREYGLPFLDTGLLYRAVGLGV
                     LDAGGSLDDEVVAVAVARALKAENLSDTERLTSGEAGEAASRTAGYPGVRAALLEFQR
                     AFAAQSGGAVLDGRDIGTVIAPEAQAKLFVTATPEVRAMRRWKQLTGRGDVIAYEDML
                     ADIVRRDERDAGRGAAPMVQADDAVLLDTTDMDIETAFDAARRIVEAARAKHGL"
BASE COUNT          320 a          713 c          877 g          346 t
ORIGIN      
        1 cgacgacgtt ctggcgacgg cgcgggcggt ggaagccttc ggcgccaagg tcgagcggct
       61 gggcgagggc cgctggcggg tgacggggcg gggcgggttc agccgaccgg ccggcgtgat
      121 cgactgcggc aatgccggaa ccggggtgcg gctgctgatg ggggcggcgg cggggtatcc
      181 gatcggcgtg accttcgacg gcgacgcgag cctgcgcaaa cggccgatga agcgggtgac
      241 ggggccgttg gccgagatgg gtgcgcggtt cggctggaag gcggaaccgg accgactgcc
      301 ggtggaagtt cagggcgggt cgctgtcggc gatcgatttt ctccagcccg tggcctcggc
      361 tcaggtgaag tcggccattc tgctggccgg gctgaacgcc gacggcgtga ctacggtagt
      421 cgaacccgaa ccgagccgcg accataccga acggatgctg cgcgccttcg gggccgaggt
      481 cggggtgctg ccggtggaag cgggctggcg cgtcagcctg aagggcggtc aggtgctgat
      541 cggaacccag gtggccgtgc cgggcgatcc atcctcggcg gccttcccgc tggcggcggg
      601 actgatcgtc ccggggtcgg aggtcacggt cgagggcgtg atgctgaatc cgctgcgcac
      661 gggtctgttc gagacctggc gcgagatggg ggccgatctg accgtctcga accgccgcat
      721 ggccggcggg gaagaggtgg gcgacgtcac ggcgcggcat tctcccttga cgggcgtggt
      781 cgtgccgccg gaacgggcgg cctcgatgat cgacgagtat ccgattctgg ccgcgaccgc
      841 cgcgttcgcg acgggctcga ccgtcatgcg gggcatcggc gagatgcggg tcaaggaaag
      901 cgatcgcatc gccctgatgg cggcgggcct gaaagcctgc ggcgtcgcgg tagaggaaga
      961 ggcggagggc ttcacggtga ccgggggccc cgttcgcggc ggggcgaccg tgaccactca
     1021 cggcgatcat cgcatcgcca tgagccatct ggtgctcggg ctggcggcgg acgaggccgt
     1081 aaccgtcgac gagccgggca tgatcgcgac gagctttcca ggatttatgg agttgatgca
     1141 gcggctcggg gcgaggatag aggccggctg atgcgatggc cgaacccacc tcaaccgccg
     1201 acgaccaggc gccccaagtc gaaaggccgt ctggtcttcg acgccgccct tttcacgggt
     1261 acggcgctgg tgatgggctg ggccggtctc gatcgcgcgc aagcggggcg ggtcacgctg
     1321 cgcagctggc ctgacgtgct ggtgaccgac cagccgggcc tcttctgggc gctgatcgcc
     1381 ggccattggg tgatcgtagc cgtgtgcctc gggttgagcg gcctggcgat ccgggcgttg
     1441 ctcgccccgt cagccaagga tgttcgccca tgaaccttgt catcgccgtc gacggccccg
     1501 ccgcctcggg caagggcact atcgccgcgc ggctggcgcg cgagtacggc ctgccgtttc
     1561 tcgataccgg ccttttgtac cgggccgtcg ggctgggcgt gctggacgca ggcggatcac
     1621 tggacgacga ggtcgtggcc gtggcggtgg cgcgggcgct caaagccgag aacctgagcg
     1681 acaccgaacg gctgaccagc ggcgaggccg gcgaggcggc cagccggacg gcgggctatc
     1741 ccggcgttcg cgcggccctg ctggagttcc agcgcgcgtt cgccgcccag tccggggggg
     1801 cggtgctgga cgggcgggac atcggcaccg tcatcgcccc ggaggcgcag gccaagctgt
     1861 tcgtgaccgc cacgcccgag gtgcgggcca tgcggcgctg gaagcagctg accggccgcg
     1921 gcgatgtcat cgcctatgag gatatgctgg ccgacatcgt gcgccgcgac gagcgggacg
     1981 cggggcgggg cgcggccccg atggttcagg ccgacgacgc cgtcttgctg gatacgaccg
     2041 atatggatat agagaccgcc ttcgatgcgg cccgccgtat cgtcgaggcg gcgcgcgcga
     2101 aacacggtct ctgacccgtt ccgcaaatcc aaccgcgcct ttccctcgat cgctgcgggc
     2161 aaatctcact gcgcggggta ttccccgcgt cttcggttgc aacccttaac ccgaccagcg
     2221 ggactccgtc cggccacgtg tggcgcggac ccgtag
//
LOCUS       DASVVN010000076         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_186324,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000076 DASVVN010000000
VERSION     DASVVN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186324"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1362)
                     /locus_tag="VF686_01230"
     CDS             complement(202..1362)
                     /locus_tag="VF686_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX8660458.1"
                     /translation="MQKLRWPIGRRGRETAPEIKDSRAGPLIALTSAGRARWTPRDYA
                     HLAVEGFGKNAVAYRCVRMIAEAAASTPLIVFADGTRAPDHPLARLLARPNPEQSGVE
                     WLEGLYGALQTAGNAYAEAVGEAEPEELWTLRPDRVKVVPGRVGWAEAWDYSVDGRSV
                     RIGRAPDGWMPVMQLKLFHPTDDHYGFSPLEAAASAIDVHNASADWNKALLDNAARPS
                     GALVYGSKDGERLTAEQFEGLRAQVNESHAGTMNAGRPMILEGGLDWKPMSWTPADMD
                     FIAGKHAAAREIALAFGVPPQLLGIPGDATYANYREANAAFWRGTVVPLVRKAAAALG
                     GWLGGRFEGIRIEADLEALPALQPERDALWARLNAAAFLTDEERRRMAGVGD"
BASE COUNT          266 a          690 c          500 g          291 t
ORIGIN      
        1 cgatccgccc cagagacgcg cgcgtggcga cgctctgctc ctccagccgg gccagccgct
       61 cggcgaccag tcgctgctcc gcgacccgtt cctccagcgt ggcgatccgc gccgccgccc
      121 cgccggccca taccagcccg ccgatcgtct gcaccgtcag cgccatgatc agtgccatcg
      181 gaatcttcgc cagaccttcc atcagtcgcc cactcccgcc attcgccgcc gctcctcgtc
      241 cgtcaggaag gccgctgcgt tcagccgcgc ccacagcgcg tcccgctccg gctgcagcgc
      301 cggcagcgcc tccagatccg cttcgatccg aatcccctcg aaccgcccgc ccaaccagcc
      361 gcccaaggcc gcagccgcct tgcggaccaa gggcaccacc gtcccccgcc agaaggccgc
      421 attggcttcg cgatagttgg cgtaggtggc atctccgggg atccccaaca gttgcggcgg
      481 aaccccgaac gccagggcga tctcgcgcgc cgcggcatgc ttgccggcga tgaaatccat
      541 gtcggccggg gtccagctca tcggcttcca gtccagcccg ccttctagga tcatcggccg
      601 gccagcgttc atcgtccccg cgtggctctc gttgacctgc gcccgcagac cctcgaattg
      661 ttcggccgtc agtcgctcgc catcttttga gccgtagacc agcgcgcccg atggccgcgc
      721 cgcattgtcc agcagcgcct tgttccagtc cgccgacgca ttgtgcacgt cgatcgccga
      781 ggccgccgcc tccagtggtg aaaatccgta gtgatcgtcg gtcggatgaa acagcttcag
      841 ctgcatcaca ggcatccagc cgtccggcgc ccggccaatc cgcaccgacc ggccgtcgac
      901 cgaatagtcc caggcttcgg cccagcctac acgccccggc acgaccttca cccggtcagg
      961 ccgcagcgtc cagagctcct cgggctccgc ctcccccacc gcctcggcat aggcgttgcc
     1021 cgccgtctgc agcgcgccgt acagtccctc cagccattcg acccccgact gctcgggatt
     1081 gggccgcgcg agcagccgcg ccagcggatg atccggcgcc ctggtcccgt cggcgaacac
     1141 gatcagcggg gtcgacgccg ccgcctcggc gatcatccgg acgcaacggt aggccaccgc
     1201 attcttgccg aacccctcga ctgccagatg ggcatagtcg cgcggcgtcc accgggctcg
     1261 tccggcgctc gtcagggcga tcagcggccc ggcgcggctg tccttgatct cgggcgcggt
     1321 ttcacgcccg cggcggccta tcggccagcg cagcttctgc attttcaccc ttccgaaggt
     1381 ccgcttcagc gcgtccgtac gccgagcccg ggaatggacc gctcggggcg tcccggcccg
     1441 gtcctgattt cccgtcatgc ccaaagacta cccgaccagc gcaccaaggc tgggaaacag
     1501 ggccgagaaa aacgtaaccc tttgtgggtg caacgtttta ttttttgaat ccgactatag
     1561 attcgccaac agacagcaca ccggtgtccg gatcgccgtg ttcaggccgc gacctcgccc
     1621 tcatcacgct ctgtgtctcc ggcgtcccgc tgcgatgcgc ggctcatcac agccagcaat
     1681 tccgtcaact ggatcggttt gccgacatgg ccgtccatac ccgccgccat gcagcgctcg
     1741 acatgtt
//
LOCUS       DASVVN010000077         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_186338,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000077 DASVVN010000000
VERSION     DASVVN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186338"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VF686_01235"
     CDS             <1..1483
                     /locus_tag="VF686_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8660459.1"
                     /translation="PFRNKWVQGPPGHESAAYHGYWITDFTQIDPHLGTNDEFRALVD
                     AAHARGMKVYLDIVINHTADVIRYRECPQNDCGYRWKGDYPYQRRGGLQGEPINPGFT
                     GAPGGDFSTLTRPDYAYTPYVPAGEESAKAPAWLNDVSLYHNRGNSAWYSEARMDGDF
                     AGLDDVFTEHPRVIQGFIDLYGSWIDDYGIDGYRIDTARHVNPEFWQAFVPAILERAR
                     AKGIPNFHIFGETYDFQSGTAAMHTVVDRLPTVLDFAYQRVVQNMVTGVQHPDAMGYL
                     LMEDAIYAEGAETALKLPTFTGNHDMGRIGHLILHAKPDISDAELLDRSTLAHALVLL
                     GRGVPVIYYGDEQGFTGDGDDRRARQDMFETRVPSYADDRRIGSASGPFDANADMYRR
                     IAEMSRVRAADKRLRHGRVVVRTADQTPGILAISRLDDDGETLVVFNTSTGARDVNVA
                     VETGSAEWRALIGSCPARSAAPGSVSVSLPALGYLVCVSERPA"
     gene            1459..>1641
                     /locus_tag="VF686_01240"
     CDS             1459..>1641
                     /locus_tag="VF686_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX8660460.1"
                     /translation="MRIRKTSVTAQILEQPAVLTDAAEWWRGAVIYQVYPRSFADTNG
                     DGVGDLAGVTGHLDHIA"
BASE COUNT          281 a          622 c          486 g          252 t
ORIGIN      
        1 gccgttccgg aacaaatggg tgcagggtcc acccggtcac gagagcgcgg cctatcacgg
       61 ctactggatc accgacttca cgcagataga cccgcatctg ggcaccaatg acgagttccg
      121 cgccctcgtc gacgccgccc atgcgcgcgg gatgaaggtc tatctggaca tcgtcatcaa
      181 tcacacggcg gacgtcatcc gctaccggga gtgcccgcag aacgactgcg gctaccgctg
      241 gaagggcgac tatccttatc agcgccgcgg cggcctgcag ggcgagccga ttaaccccgg
      301 cttcaccggc gccccgggcg gcgacttctc gaccctgacc cgccccgact atgcgtacac
      361 gccctacgtc cccgccggag aggagtccgc caaggccccg gcctggctga acgacgtcag
      421 cctctatcac aaccggggca acagcgcctg gtacagcgaa gcccgcatgg acggcgactt
      481 cgccggtctc gacgacgtgt tcaccgaaca cccgcgcgtc atccagggct tcatcgacct
      541 ctacggcagc tggatcgacg actacggcat cgacggctac cgcatcgaca cggcccgcca
      601 cgtgaacccc gagttctggc aggccttcgt ccccgccatc ctcgaacgcg cccgggcgaa
      661 gggcatcccg aacttccaca tcttcggcga gacctatgac ttccagtccg gcacggcggc
      721 catgcacacc gtggtcgacc gcctgccgac ggtgctcgac ttcgcctacc agcgcgtcgt
      781 ccagaacatg gtcacgggcg tccagcaccc cgacgccatg ggctatctgc tgatggagga
      841 cgctatctac gccgaaggcg cggagacggc gctgaaactg ccgaccttca ccggcaacca
      901 cgacatgggc cggatcggcc atctgatcct gcacgcgaag cccgacatct ccgacgccga
      961 actgctggac cgctcgaccc tggcccacgc cctggtcctg ctcggccgcg gcgtgccggt
     1021 catctactat ggcgacgaac agggcttcac cggcgatggc gacgaccgcc gcgcgcggca
     1081 ggacatgttc gagacccgcg tgccctccta tgccgacgac cgccgcatcg gctccgcctc
     1141 cggtccgttc gacgccaacg ccgacatgta tcgccgcatc gccgagatga gccgcgtccg
     1201 cgccgccgac aaacgcctgc gccatggccg cgtcgtcgtg cgcaccgccg accagacgcc
     1261 cggcatcctc gccatctcgc gtctggatga tgacggcgag acccttgtgg tgttcaacac
     1321 ctcgaccggg gcgcgcgacg tcaatgtcgc cgtcgagacc ggctccgccg aatggcgcgc
     1381 cctgatcgga tcgtgccccg cccgcagcgc cgctccgggc tcagtttccg tgtccctgcc
     1441 cgcgttaggc tatcttgtct gcgtatccga aagaccagcg tgaccgccca gattctcgaa
     1501 cagcccgccg tcctgaccga cgccgccgaa tggtggcgcg gcgccgtcat ctatcaggtc
     1561 tatccgcgca gtttcgccga caccaacggc gacggcgtcg gcgatctggc cggcgtcacc
     1621 gggcatctgg accatatcgc c
//
LOCUS       DASVVN010000078         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_213350,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000078 DASVVN010000000
VERSION     DASVVN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213350"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF686_01245"
     CDS             complement(<1..435)
                     /locus_tag="VF686_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX8660461.1"
                     /translation="MTLRPFLPTLCALAALTAACDGDNSVRITSTRNESDAKGVLKVV
                     ETLQCPQTMGSLTRKGSAQDGGKVCTYVGRRGTEVSLHLVALDGDAPADVLKAFESRL
                     SAALPGAVAQLKASADAEKARMQADLARANADTAAAAADAAGA"
     gene            complement(535..1407)
                     /locus_tag="VF686_01250"
     CDS             complement(535..1407)
                     /locus_tag="VF686_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8660462.1"
                     /translation="MSRSSDRFEGTSNYIATDDLKIAVNAAIALERPLLIKGEPGTGK
                     TVLAYEIARALNAPLITWHIKSTTKAHNGLYEYDAVSRLRDSQLGEARVHDVRNYLKP
                     GKLWEAFTAPNRPVLLIDEIDKADIEFPNDLLQELDRMEFYVQETDETIRAEVRPVVI
                     ITSNNEKELPDAFLRRCFFHYIRFPDDQTMRAIVEVHFPGIKPRLVSEALKTFYEIRD
                     TPGLKKKPSTSELLDWLKLLLVDDIDPETLREKSPNKLIPPLHGALLKNEQDVHLFER
                     LAFLGRREGSRPGS"
BASE COUNT          235 a          447 c          610 g          309 t
ORIGIN      
        1 ggcgccggct gcgtcagccg cagcggccgc ggtgtcggcg ttggcgcggg cgagatcggc
       61 ctgcatgcgg gccttttcgg cgtccgccga ggccttcaac tgggcgacgg ccccgggaag
      121 ggcggccgac aggcgggatt cgaaggcctt caggacgtcg gcgggtgcgt ctccgtcgag
      181 ggcgacgagg tgcaggctga cctccgtccc cctgcggccg acataggtgc agaccttgcc
      241 gccgtcctgg gccgagccct tgcgggtcag ggagcccatg gtctgggggc attgcagggt
      301 ctcgacgacc ttgagcacgc ccttggcgtc gctttcgttg cgcgtcgagg tgatccgcac
      361 cgagttgtcg ccgtcgcagg cggcggtcag ggcggcgagg gcgcagaggg tgggcagaaa
      421 gggacgcagg gtcatcttcg ggtagtcctg ttcgtggtcc tatcctgagc gcgcggggcc
      481 gataatccag tgaaatcgcg gtaaggcctg tgaccctgcc gcaacccgac tgtgtcagct
      541 cccgggccgg gacccctcgc ggcggccgag gaaggcgagg cgttcgaaca ggtggacgtc
      601 ctgttcgttc ttcagcaggg cgccatgcag gggcgggatc agtttgttgg gcgatttctc
      661 gcgcagggtt tcgggatcga tgtcgtcaac cagcagcagt ttcagccagt cgagcagctc
      721 cgaggtcgag ggcttcttct tcaggccggg cgtgtcgcgg atttcgtaga aagtcttgag
      781 ggcctccgag accagccgcg gcttgatgcc ggggaaatgg acctcgacga tggcgcgcat
      841 cgtctggtcg tcggggaagc ggatgtagtg gaagaagcag cggcgcagga aggcgtcggg
      901 cagctccttc tcgttgttgg aggtgatgat gacgacgggg cggacctcgg cgcggatcgt
      961 ctcgtccgtc tcctggacat agaactccat gcggtcgagt tcctgcagca ggtcgttggg
     1021 gaattcgatg tcggccttgt cgatctcgtc gatcagcagg acgggacggt tcggggcggt
     1081 gaaggcctcc cacagcttgc cgggcttgag gtagttgcgc acgtcgtgga cgcgcgcctc
     1141 gcccagctgg ctgtcgcgca ggcgcgagac ggcgtcgtat tcgtacaggc cgttgtgggc
     1201 cttggtcgtg gatttgatgt gccaggtgat caggggggcg ttcagggccc gggcgatctc
     1261 ataggccaag acggtcttgc cggtgccggg ctcgcccttg atcagcaggg ggcgttccag
     1321 tgcgatggcg gcgttgaccg cgatcttcag gtcgtcggtg gcgatatagt tcgaagtgcc
     1381 ttcgaaacga tcggacgaac gggacatggt cggctccggc gggtgtccgg ttggggttag
     1441 cgaaggcggg gcgggttaac aacgatcatg tgggttcggg cggggtgtct tctttccggc
     1501 ggcgcggcgt tccatggctt ccagctggaa caggggcttc gacaggacga agtcctcgat
     1561 atcgaccgga tggccggccc ggacgatgcg catatacatc t
//
LOCUS       DASVVN010000079         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_134529,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000079 DASVVN010000000
VERSION     DASVVN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134529"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF686_01255"
     CDS             complement(<1..317)
                     /locus_tag="VF686_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8660463.1"
                     /translation="MRSFDSLSRRGLLLSLGAGGLVAGSSRQALADPVFAEYPFQLGI
                     AAGDPAPDGFVIWTRLAPRPLEIGHGMPSQPVAVKWEIATDRGFSNVAQKGEAVARPE
                     MGHA"
     gene            complement(370..795)
                     /locus_tag="VF686_01260"
     CDS             complement(370..795)
                     /locus_tag="VF686_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX8660464.1"
                     /translation="MRSAFVFLLAGFALAACDGGGDPVQQALRETATANQSAVVKETA
                     TTSRPGHGSTSHGTSAGDRAFAAAMHAGMAAASGETLDAAYIAKMIEHHRGAVAMADV
                     ALARSQDAEIRRMAQAVKAAQTREIAEMQAWKPTGPSGQ"
     gene            complement(795..>1882)
                     /locus_tag="VF686_01265"
     CDS             complement(795..>1882)
                     /locus_tag="VF686_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX8660465.1"
                     /translation="DRPWVQPALQPRLAPADVPAPESWADAVLKVLTCPDMASKRWIW
                     EQYDRHVMADTLHDSATAADAGVVRVHGTDKGLAMTSDVTPRYVQADPYEGGKQAVAE
                     AWRNLTAVGSRPIAITDNLNFGNPQRPEIMGQIVRAIDGMAEACRVLDFPVVSGNVSL
                     YNETNGAAIPPTPTVGAVGLLTNYDVVVGFGGMVEGDVLVLIGETVGELGSSIYLREV
                     LGREDGAPPPVDLALERVTGDFVRGLIEAGDLTCVHDLSDGGLIGAAADIALASDCGV
                     ELDATSATHAHILLFGEDQARYLVATADATGIIAAAQKAGLHASVVGRAKGRDFASGG
                     PDGDLFRLPLDHLREMHEAWMPNWIEG"
BASE COUNT          276 a          661 c          655 g          290 t
ORIGIN      
        1 gcgtggccca tctcgggccg ggcgacggct tcgcccttct gcgccacgtt ggagaagccg
       61 cggtccgtcg cgatttccca ttttaccgcc accggctggg acggcatgcc gtggccgatc
      121 tcaagcgggc gcggagccag ccgggtccag atcacgaagc cgtcgggagc aggatcgccg
      181 gcggcgatgc ccagttggaa cggatattcg gcgaacaccg gatcggccag cgcctgtcgg
      241 ctcgatcccg cgacgagccc cccggcgccg aggctcaaca gcagcccgcg acgggacagg
      301 gaatcgaacg agcgcatggc ggcctccggt cagcgaaggc tccgacctag cccggaaata
      361 tgacggtcgt cactgcccgg atgggccggt gggcttccat gcctgcattt ccgcgatctc
      421 gcgggtctgc gcggccttga ccgcctgcgc catgcggcgg atttcggcgt cctgcgagcg
      481 cgcgagagcg acatccgcca tggccaccgc gccgcgatga tgctcgatca tcttggcgat
      541 ataggccgcg tccagggtct cgcctgacgc cgccgccata cccgcgtgca tggcggctgc
      601 gaaggcccgg tcgccggcgg acgttccgtg actcgtcgag ccatggccgg ggcgcgaggt
      661 cgtcgctgtt tccttgacga cggccgactg gttggctgtc gcggtctcgc gcagggcctg
      721 ctgcaccgga tcgccgccgc cgtcgcaggc ggcgagggcg aaacccgcta ggaggaagac
      781 gaaggcgctg cgcatcagcc ttcgatccag ttcggcatcc aggcctcgtg catctcgcgc
      841 agatgatcca gcggcaggcg gaacaggtcg ccgtccggtc cgcccgaggc gaagtcccgg
      901 cccttggccc ggcccaccac cgaagcatgc agtccggcct tttgcgccgc ggctatgatg
      961 ccggtcgcgt ccgcagtggc gaccagatag cgggcctgat cctcgccgaa gagcaggatg
     1021 tgggcgtggg tcgcagaggt ggcgtccagc tcgacgccgc agtccgaggc cagggcgatg
     1081 tccgccgccg ccccgatcag gccgccgtcc gacaggtcgt ggacgcaggt caggtcgccg
     1141 gcctcgatca ggccgcgcac gaagtcgccc gtgacccgtt ccagcgccag gtcgaccggc
     1201 ggcggcgcgc cgtcctcgcg gcccagcacc tcgcgcagat agatggacga gcccagctcg
     1261 ccgaccgtct cgccgatcaa caccagcacg tcgccctcga ccatgccgcc gaagccgacg
     1321 acgacgtcgt agttggtcag caggccaacc gctccgaccg tcggggttgg cggaatggcc
     1381 gcgccattgg tctcgttata gaggctgacg tttccgctca cgaccgggaa gtccagcacg
     1441 cggcaggctt cggccatgcc gtcgatcgcg cggacgatct ggcccatgat ttcgggccgc
     1501 tgcggattgc cgaagttcag gttgtcggtg atggcgatgg gccggctgcc cacggcggtc
     1561 aggttgcgcc atgcctccgc caccgcctgt ttgccgccct cgtagggatc ggcctgcaca
     1621 tagcgcggcg tcacgtccga ggtcatagcc aagcccttgt cggtgccgtg cacccgcacc
     1681 acgccagcgt cggcggcggt ggccgagtca tgcagggtgt cagccatgac gtgccggtcg
     1741 tactgctccc agatccatcg cttcgaggcc atgtcggggc aggtcagcac cttcagcacc
     1801 gcatcggccc aactttcggg ggcgggcacg tcggctggcg caaggcgcgg ctgcagcgcg
     1861 ggctggaccc acgggcggtc gt
//
LOCUS       DASVVN010000080         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_81867, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000080 DASVVN010000000
VERSION     DASVVN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81867"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VF686_01270"
     CDS             complement(<1..1380)
                     /locus_tag="VF686_01270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX8660466.1"
                     /translation="MTLRLSSGTMGALPSQVARPGYDRSQVRIGVVHLGIGAFHRAHQ
                     AVVFDDTLNAGDLRWGVLGASLRSPDVRNQLAPQDGLYTLVVEDGAARSMRVIGAVQA
                     VMVAPEDPAALVEAMASPDVHIVTLTVTEKGYYLDPASGTLKLDDRAVAADLADLSAP
                     VTAPGFVTAALGLRRSRGLPAFTVISCDNLPHNGARIRSAVLEMARRTDPELSDWIAA
                     EGAFPQTMIDRIVPATTPEDIAALTAELGVADHGMVKTEPFTQWIIEDRFAGERPDFA
                     RFGVQLTEAVGPWENAKLRLLNGAHSALAYLGALAGFDHVHEAVAVPALRAFVDRLWD
                     EAETTLSPPSGLDIAVYRAELMARFSNAALRHRTRQIAMDGSQKLPQRLLATAQARLA
                     AGQPIPALALAIAAWMRWQEGRSEAGEIVAVEDPLAAETARRLSTASDDADKVAALLD
                     FEAVFPQALS"
     gene            complement(1377..2792)
                     /gene="uxaC"
                     /locus_tag="VF686_01275"
     CDS             complement(1377..2792)
                     /gene="uxaC"
                     /locus_tag="VF686_01275"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX8660467.1"
                     /translation="MTQPLHLHPDRLFPSDPDTRAVARRLHAEIRDLPIISPHGHTDP
                     SWFAGNAPFANPAELLITPDHYVFRMLFSQGVPMEDLGVPRTDGGAVETDPRAIWRRF
                     AERYHLFHGTPSRIWHDWVYAEAFGIDVRLSAETADHYYEVIDAALKTEAFRPRALFE
                     RHNIEVLTTTESPIDALEHHQAIRASGWKGRVLTAYRPDPVLDPDYEGFHANLAILAD
                     QTGRDTLSWAGYLDAHRDRRAFFAAMGATSTDHGHPTAFTADLSRADAEALFARVSKG
                     PVSVADAELFRGQMLTEMAAMSVDDGLVMQLHPGSFRNHNAAVFRQFGRDKGADIPTR
                     TDYVQALKPLLDRFGSDPRLSLIVFTLDETTYSRELAPLAGHYPALKLGPSWWFHDSP
                     EGMRRFREQVTETAGFRNTVGFNDDTRAFLSIPARHDVARRMDCGFLAKLVVEHRLEE
                     DEAHDLARALTYDLVKAAYKL"
BASE COUNT          519 a         1041 c         1158 g          506 t
ORIGIN      
        1 cgaaagcgct tgagggaaca ccgcctcgaa atccagcagg gcggcgacct tgtctgcgtc
       61 gtcagaggcg gtcgacaggc ggcgcgcggt ctccgccgcc agagggtcct cgacggcgac
      121 aatctcgccg gcctcgctgc gtccctcctg ccagcgcatc caggcggcta tggcgagggc
      181 gagggcggga atgggctggc cggcggcgag acgcgcctgc gcggtggcca gcagccgctg
      241 cgggagcttc tgcgatccgt ccatcgcgat ctgccttgtc cggtgccgca gcgccgcgtt
      301 cgagaaccgc gccatcagct cggcgcgata gacggcgatg tcgagaccgg acggcgggct
      361 gagcgtggtc tccgcttcgt cccagaggcg gtcgacgaag gccctcagcg ccggaacggc
      421 gacggcctcg tgcacatggt cgaacccggc caaggcgccc aggtaggcga gggcggaatg
      481 ggccccgttg agcagccgca gcttggcgtt ctcccacggg ccgacggcct ccgtcagctg
      541 aaccccgaac cgggcgaagt cagggcgctc gccggcaaag cggtcctcga tgatccattg
      601 ggtgaagggt tcggtcttga ccatgccgtg gtcggcgacg cccagttcgg cggtcagggc
      661 cgcgatgtct tcgggggtcg tggccggcac gatgcggtcg atcatggtct gggggaaggc
      721 cccctcggcg gcgatccagt cgctcagctc cgggtcggtc cggcgcgcca tctccagcac
      781 cgccgaccgg atgcgtgcgc cgttgtgcgg aaggttgtcg cacgaaatga cggtgaaggc
      841 cggcaggccc cggctgcggc gcagtccaag cgcggcggtc acgaagccgg gcgccgtgac
      901 gggcgcggac aggtcggcca gatcggccgc gacggcgcgg tcgtccagct tcagcgttcc
      961 ggaggccggg tccagataat agcccttctc ggtcacggtc agggtgacga tgtggacgtc
     1021 gggcgaggcc atggcttcca ccagcgccgc cgggtcttcg ggtgcgacca tgacagcctg
     1081 caccgcgccg atgacccgca tcgaccgcgc ggccccgtcc tcgaccacca gggtgtagag
     1141 cccgtcctgg ggcgccagct ggttgcgaac atcgggcgat cgcagcgacg cgcccagcac
     1201 gccccagcgc agatcgccgg cgttcagcgt atcgtcgaag acgactgcct gatgagcccg
     1261 gtgaaaggcg ccgatgccga ggtgcaccac cccgatccgg acttggctcc ggtcgtaccc
     1321 ggggcgcgcg acctgcgagg gcagggcgcc catcgttccg gaactgagcc gcagggtcat
     1381 agcttgtacg ccgccttgac cagatcatag gtcagggcgc gggcgaggtc gtgcgcctcg
     1441 tcctcttcca gccggtgctc gacgacgagt ttggcgagga agccgcagtc catgcgccgg
     1501 gccacgtcgt gacgggcggg gatggacagg aaggcgcggg tgtcgtcgtt gaagccgacg
     1561 gtgttgcgga agccggccgt ctcggtcacc tgttcgcgga accggcgcat cccctccggg
     1621 ctgtcgtgga accaccagct gggccccagc ttcagcgccg gatagtgacc cgccagcggc
     1681 gccagctcgc ggctgtaggt cgtctcgtcg agggtgaaga cgatcagcga cagccgcggg
     1741 tccgagccga agcggtcgag caggggcttc agcgcctgga catagtcggt gcgcgtcgga
     1801 atgtccgcgc ccttgtcgcg gccgaactgc cggaagacgg cggcgttgtg gttgcggaag
     1861 ctgccgggat gcagctgcat caccagaccg tcgtccacgc tcatggccgc catttcggtc
     1921 agcatctggc cgcggaacag ttcggcgtcg gccacgctga cgggcccctt ggagacccgg
     1981 gcgaacaagg cctcggcgtc ggcacgcgac aggtcggcgg tgaaggccgt cggatggccg
     2041 tggtccgtcg aggtcgcgcc catggcggcg aagaatgctc tccggtcgcg gtgcgcgtcg
     2101 agataaccgg cccagctcag ggtgtcccgc ccggtctggt cggccaggat cgccagattg
     2161 gcgtggaagc cttcgtagtc cggatcgagc acggggtcgg gccgataggc ggtcagcacg
     2221 cgccccttcc agcccgaggc ccggatcgcc tgatgatgct ccagcgcatc aatcggactt
     2281 tcggtggtgg tcaggacctc gatgttgtga cgctcgaaca gggcgcgcgg ccggaaagct
     2341 tcggtcttca gggcggcgtc gatgacctcg taatagtgat ccgccgtttc ggcgctcagc
     2401 cgcacatcga tcccgaacgc ctcggcatag acccagtcgt gccagatgcg ggacggggtg
     2461 ccgtggaaca ggtgatagcg ctcggcgaac cggcgccaga tcgcgcgggg atcggtctcc
     2521 accgcgccgc cgtcggtccg cggcacgccg agatcctcca tcggcacgcc ctgactgaac
     2581 agcatgcgga agacatagtg gtcgggcgtg atcaggagtt cggcgggatt ggcgaacggc
     2641 gcattgcccg cgaaccacga cggatcggta tggccgtgtg ggctgatgat cggcaggtcg
     2701 cggatttcgg catgcagacg gcgggcgaca gcgcgggtgt ccggatcaga cggaaacagg
     2761 cggtcagggt gaagatggag aggctgcgtc acgtcttgat cccgatcact gaaagccgac
     2821 ggccgtcact gtccggcgcg ccgcggtgca gaaagacacc ggttaccaca acgcgcaaca
     2881 gatcgatcgg cccgccggta aattgacccg cgcggatcgc tgactcggca tcagaatgct
     2941 gcgcctgcga gcggcaaggc ggcaccgttg cgcacgggtt cctcaggaaa ttcggcaact
     3001 aacatgccga tatcgcagag gttgtcgcga attttgcaat aatttctcgc aaaacatcgt
     3061 ccgcccactt gtctatttcg caaatgcggt aaacctttcg cacatgcgta acgctgtcct
     3121 gggggatcaa atgacacgac ttcgtatcgg gcttgcgggg gctctgaccc tcggggtgag
     3181 cggtctggcg gggacggctt ccgccgccgc gcccgccgct ccgg
//
LOCUS       DASVVN010000081         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_265879,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000081 DASVVN010000000
VERSION     DASVVN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265879"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VF686_01280"
     CDS             <1..1378
                     /locus_tag="VF686_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX8660468.1"
                     /translation="AAWARWVRPWTLAAFSLLTVGITLGAFWAYYELGWGGWWFWDPV
                     ENASFMPWLAGAALLHSAVVTERRGALAGWTTFLALLAFTFSMLGAFLVRSGVLTSVH
                     AFAVDPERGLMLLAILGVTAGAAFALFAWRAPRLTGGGQFAPVSREGALVLNNLFLTA
                     ACATVLLGTLYPLILEAMNGATISVGPPYFALTFVPLMAVAFLILPAGPLLAWKRGDL
                     KGVGQRLAVAAGLALLVALAGWMAFEPRKALSAAGIGLGGWLVLGALAEIAERVKLFR
                     VGLPESLRRAGGLPLGAWGTVLAHAGLGVFVLGAVVETGYRVEAARTVAVGETVTAGR
                     YSVRLDDVRVVEGPNYLAERGRLTVSLRDGGAARVVTAERRFFPAGGQTTTEVGLDFR
                     GLDDIYVVLGERGQTAAGAPAWNVRVWWNPWARLIFLGPAIMALAGVLSLLDRRLRLG
                     VGKRRAAA"
     gene            1375..>1760
                     /locus_tag="VF686_01285"
     CDS             1375..>1760
                     /locus_tag="VF686_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX8660469.1"
                     /translation="MKRALLIALAAVGLMAAEPPPVPDRPLPDAAQEARAKALFADIR
                     CVVCQHEAISDSPAGIAADMRRLVREEIAAGRTDAEVREDLVRRYGDFVLFKPPVRAG
                     TWLLWFGPLAALIAVGIGLLAVSRRRR"
BASE COUNT          175 a          622 c          660 g          303 t
ORIGIN      
        1 cgcagcctgg gcgcgctggg tgcggccctg gacgctggcg gctttcagcc tgctgaccgt
       61 ggggatcacg ctgggcgcct tctgggccta ttacgagctg ggctggggcg gctggtggtt
      121 ctgggatccg gtcgagaacg ccagcttcat gccatggctg gccggtgcgg ctctgctgca
      181 cagcgccgtg gtcaccgagc ggcgcggggc gctggccggc tggacgacct tcctggccct
      241 gctggccttc accttctcca tgctgggggc cttcctcgtg cgctccggcg tgctgaccag
      301 cgtccatgcc ttcgccgtcg atcccgagcg cggcctgatg ctgctggcca tcctcggcgt
      361 caccgcaggt gcggccttcg ccctgttcgc ctggcgggcg ccgcgtctga cgggcggcgg
      421 ccagttcgcc ccggtcagcc gcgagggcgc gctggtgctg aacaacctgt tcctgacagc
      481 ggcctgcgcc accgtgctgc tgggcaccct ctatccgctg atcctcgagg ccatgaacgg
      541 cgcgaccatc tcggtcggcc cgccctattt cgccctgacc ttcgtgcccc tgatggccgt
      601 ggccttcctg atcctgcctg ccgggccgct gctggcgtgg aagcgcggcg atctgaaggg
      661 cgtgggccaa cgactggctg tcgccgcagg gctggcgctt ctggtcgcgt tggcgggatg
      721 gatggcgttc gagccacgca aggcgctgtc cgcggccggg atcggtctcg gcggctggct
      781 ggtgctcggc gccctggccg agatcgcgga gcgggtgaag ctgttccgtg ttggactgcc
      841 ggagagcttg cgccgcgccg gaggtctgcc gctgggggcc tggggcacgg ttctggccca
      901 cgccgggctg ggagttttcg tgctcggcgc ggttgtcgag accggctacc gcgtcgaggc
      961 ggcgcggaca gtggccgtcg gcgaaaccgt gacggccggt cgctacagcg tgcggctgga
     1021 cgacgtccgc gtcgtcgagg gccccaacta tctggcggag cggggccggc tgaccgtctc
     1081 cctgcgcgac ggcggcgcgg cgcgcgtcgt cacggccgag cgccgtttct tcccggcggg
     1141 cggccagacc accaccgagg tcgggctgga tttccggggt ctggacgaca tctatgtcgt
     1201 gctgggcgaa cggggccaga ccgccgccgg cgcgcccgcg tggaacgtgc gcgtctggtg
     1261 gaatccgtgg gcgcggctga tcttccttgg tcccgccatc atggctctgg ccggggtcct
     1321 ttcactcctc gaccggcggc tgaggctcgg cgtcggcaag cgcagggcgg cggcgtgaag
     1381 cgcgccctgc tcatcgccct ggccgccgtc ggcctgatgg ccgccgaacc accgcccgtg
     1441 cccgaccggc ccttgccgga cgccgcgcag gaggcccgcg ccaaggccct gttcgccgac
     1501 atccgctgcg tcgtctgcca gcacgaggcc atctccgaca gcccggccgg catcgccgcc
     1561 gacatgcgcc ggctggtgcg cgaggagatc gccgcgggcc gcacggacgc cgaggtccgc
     1621 gaggatctgg tgcgccgcta cggcgatttc gtgctgttca agccgcccgt gcgcgccggg
     1681 acatggctgc tgtggttcgg ccctctggcg gccctgatcg ccgtcgggat cggcttgctg
     1741 gccgtgtcgc gccgccgccg
//
LOCUS       DASVVN010000082         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_371240,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000082 DASVVN010000000
VERSION     DASVVN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371240"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VF686_01290"
     CDS             complement(<1..797)
                     /locus_tag="VF686_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX8660470.1"
                     /translation="MAVLVIADRDGDAVRDTTHKTVTAALKLSGEVDVLVIGQGSKAA
                     ADAAAKIAGVRKVLLAESAGLGHQLAEAVEATVTGLAGGYDAILSPATTDGKNFMPRI
                     AAKLDVAPISDIIEVVSNDTFVRPIYAGNALETIQSGDAKKVITVRPTSFDAAGEGGS
                     ASVETVAGADAGKAAFVSEEMVKSDRPELGAAKIVVSGGRALGSAEEFHAVMDPLADK
                     LGAAVGASRAAVDAGYAPNDYQVGQTGKVVAPALYIAIGISGAIQHLA"
     gene            complement(797..1561)
                     /locus_tag="VF686_01295"
     CDS             complement(797..1561)
                     /locus_tag="VF686_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX8660471.1"
                     /translation="MKVLVPVKRVIDYNVKARVKADQTGVDLANVKMSMNPFDEIAVE
                     EAVRMKEGKEHHAAGTVSEIVVVSIGVTQAQETIRTALAMGADRGLLIQSDADLEPLA
                     VAKLLKAVVDEEKPDLVLLGKQSIDGDNNAVGQMLAALLDWPQATFAGKIVLEGGKAT
                     VTREVDGGLQTLSATLPAVVSVDLRLNTPRYASLPNIMKAKKKEIAVKAVADYGVDTA
                     PRLTVKKVTAPPTRSAGIKVADAAELVFKLKTAGVL"
     gene            1684..2016
                     /locus_tag="VF686_01300"
     CDS             1684..2016
                     /locus_tag="VF686_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660472.1"
                     /translation="MNRLLTMKRLSILFLSTFAVLIAGMFAYENLVVAPGDRCEAEGK
                     WWDPEGRECAKPISIAEITGRPLPGQRAAASAEKNRELVAIEDSLTAQQKARDAEADR
                     QRAALAAQ"
     gene            complement(2422..2994)
                     /locus_tag="VF686_01305"
     CDS             complement(2422..2994)
                     /locus_tag="VF686_01305"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX8660473.1"
                     /translation="MVVLNRIYTKTGDGGQTRLASGATVSKSDRRVEAYGSVDELNAV
                     IGVARQHSGQHDRIDAMLGRIQNELFDLGADLATPLEPAPKWEALRILDSQVERLEAE
                     IDWMNESLKELDSFILPGGSALSAHLHLARTVARRAEREAVRLVEDGEAVTPASVRYL
                     NRLSDHLFVAARRANANGADDVKWKPGATR"
     gene            complement(2998..3204)
                     /locus_tag="VF686_01310"
     CDS             complement(2998..3204)
                     /locus_tag="VF686_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX8660474.1"
                     /translation="MGDVLNILILLAIAAVAITLGFGIYSLYRGGDFARSHSNKLMRL
                     RVALQALAIVLLCVGMWWKSTHGA"
BASE COUNT          542 a         1196 c         1114 g          582 t
ORIGIN      
        1 gccagatgct ggatggcgcc ggagatgccg atggcgatgt agagcgccgg ggccacgacc
       61 ttgccggtct ggccgacctg atagtcgttc ggcgcatagc cggcgtccac ggcggcgcgc
      121 gaggcgccca cggcggcgcc cagcttgtcg gccagcgggt ccatgacggc gtggaactcc
      181 tcggccgaac cgagcgcccg gccgcccgag acgacgatct tcgctgcgcc cagttccgga
      241 cggtccgact tgaccatctc ctcgctgacg aaggccgcct tgccggcgtc tgcgcccgcg
      301 acggtctcga ccgaagcgga accgccttca ccggcggcgt cgaagctggt cgggcgcacg
      361 gtgatgacct tcttggcgtc acccgactgg atcgtttcca gcgcattgcc ggcgtagatc
      421 gggcgcacga aggtgtcgtt cgacaccacc tcgatgatgt ccgagatcgg cgccacatcc
      481 agcttggcgg cgatgcgcgg catgaaattc ttgccgtcgg tggtggccgg cgaaaggatg
      541 gcgtcatagc cgccggccag accggtcacg gtcgcctcga ccgcctcggc cagctgatgg
      601 cccagaccgg cgctctcggc cagcagcacc ttgcgcacgc cggcgatctt cgcggcggcg
      661 tcggcggccg ccttcgagcc ctggccgatg accagcacgt ccacctcgcc cgacagcttc
      721 agcgcggcgg tgacggtctt gtgggtggtg tcgcggacgg catccccgtc gcggtcagcg
      781 attacgagaa cggccattac aggacccccg cggtcttgag cttgaagacg agttcggcgg
      841 cgtcggccac cttgatgccg gccgagcggg tgggcggggc cgtgaccttc ttcaccgtca
      901 ggcggggcgc cgtgtcgacc ccgtagtcgg cgaccgcctt gactgcgatc tccttcttct
      961 tggccttcat gatgttgggc agggaggcgt agcgcggcgt gttcaggcgc aggtccaccg
     1021 acaccaccgc cggcagggtc gccgacaggg tctgcaggcc gccgtcgacc tcgcgcgtca
     1081 ccgtcgcctt gccgccttcc agaacgatct tgccggcgaa ggtggcttgg ggccagtcca
     1141 gcagggccgc cagcatctgg ccgaccgcgt tgttgtcgcc gtcgatcgac tgcttgccca
     1201 gcaggaccag atccggcttc tcctcgtcga ccaccgcctt cagcagcttg gcgacggcca
     1261 gcggctccag atcggcgtcc gactggatca gcaggccgcg gtccgcgccc atggccaggg
     1321 ctgtgcggat ggtttcctgg gcctgggtga cgccgatgga gacgaccacg atctcgctca
     1381 ccgtaccggc ggcgtgatgc tccttgcctt ccttcatgcg gacggcttcc tcgacggcga
     1441 tctcgtcgaa cggattcatg ctcatcttga cgttggccag atcaacgccg gtctggtccg
     1501 ccttgacgcg agccttgacg ttatagtcga tcacccgttt gacggggacg agaaccttca
     1561 ttgtgatatc cacgaaagcg ccggaatgtt gcgtcaggga atggtacgcc gcatccggcc
     1621 tgtcaacgta acgccgcgtc aacgaccggg ttcggtcgcc ctcaaagcca cgggaatccg
     1681 cagatgaacc gcctcctgac catgaaacgg ctgagcatcc tgtttctctc gaccttcgcc
     1741 gtgctgatcg ccggcatgtt cgcctatgag aacctggtcg tcgcgccggg cgaccgctgt
     1801 gaggccgaag gcaagtggtg ggacccggaa ggccgggaat gcgccaagcc gatctccatc
     1861 gccgagatca cgggccgccc cctccccggt cagcgcgccg ccgcctctgc cgagaaaaac
     1921 cgcgagctgg tggccatcga ggactccctg accgctcagc agaaagcccg cgacgccgag
     1981 gccgaccgcc agcgcgcggc cttggcggcg cagtaggttc tgacccttgt catccccgac
     2041 gccgcgaagc ggcgatcggg gacgctcaac cccacccact tcagcctccc ggaagctgcg
     2101 tcagcagcta tccggaagca gggctcgggc agccgcctgt ctcccggaca atcgcgccgc
     2161 gatttccggg aggtctcgcc catgagcgcg gtccgtcccg gatcgccgct tcgcggcgtc
     2221 cgggatgaca aaccctacgt ccaaatctga cgcatgcgca tgcttcgctg accgaaaccc
     2281 ccatcgggta cggtctttga aaatcgaatc cacgcccgaa ccggactcag tggactcttc
     2341 ggactcttcg gacggtgttt tttcgtcggg cccagtccgc actctgcgca tgcttcgcac
     2401 ggcgttttgg ccgacaaccg gctagcgcgt agcgcccggc ttccacttca cgtcatcagc
     2461 cccgttggcg ttggcccggc gggcggcgac gaacagatgg tctgagagcc ggttcagata
     2521 gcggaccgaa gcgggcgtca ccgcctctcc gtcctcgacc agacggacgg cctcgcgctc
     2581 ggcccggcgg gccacggtgc gggccagatg cagatgcgcc gacagggccg atccgcccgg
     2641 caggatgaag ctgtccagtt ccttcaggct ctcgttcatc cagtcgattt cggcttccag
     2701 ccgctcgacc tggctgtcga ggatgcgcag cgcctcccat ttgggcgcgg gctccagcgg
     2761 cgtggccaga tcggcgccga gatcgaacag ttcgttctgg atgcggccca gcatggcgtc
     2821 gatgcggtcg tgctggcccg aatgctgccg cgccacgccg atgacggcgt tcagctcgtc
     2881 cacggaacca taggcctcga cccggcggtc gctcttggac acggtcgcgc ccgaggccag
     2941 acgcgtctgc ccgccgtcgc cggtcttcgt atagatgcga ttcaggacga ccacggccta
     3001 ggcgccgtgg gtggatttcc accacatccc gacgcacagc agcacgatgg ccagcgcctg
     3061 cagcgcgacc ctcagccgca tcagcttgtt ggaatgcgac cgcgcgaaat cgccgccccg
     3121 gtacagcgaa tagatgccga agccgagggt gatggccacc gccgcgatgg ccagcaggat
     3181 caggatgttc agaacgtcgc ccatggccgc aatctaggcg accccgaccg gcgaggccag
     3241 cgggcgcgac gccgcaaccg ccttcgtgag cacagctaag ccgttggtca tcaattcgtg
     3301 gttcgttgaa cggcctcaag ggggcccgat gaccgaccgc gcgatccgca atctggccca
     3361 cctgcggcgg tccgcctcga ccgcccgcgt gctcaatctg ctcaatgtgt tcaatgagca
     3421 cggtcgggag ccgg
//
LOCUS       DASVVN010000083         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_345151,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000083 DASVVN010000000
VERSION     DASVVN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345151"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..1180
                     /locus_tag="VF686_01315"
     CDS             482..1180
                     /locus_tag="VF686_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8660475.1"
                     /translation="MKRIGLVFLLLAAGCTQPPDPRGVRPNEVLLQVAATGRAEARPD
                     EARLTVGVSTNAPSAAAASAGNNAAMARVSAALARLGVKPDDIQTRSVTLGRVEYGPG
                     RGGYRAENMVEVRIRDVRQAGPAISAATEAGGNVVSGPDLQASDPERAKNAAYAAAYK
                     AARARAEAYAAAAGLKVERVIAIRDGSGQPPAPWYMGDARGAVAQSVAPAPPVHAGAS
                     TYEVQVHADFALAR"
     gene            complement(1191..>3125)
                     /locus_tag="VF686_01320"
     CDS             complement(1191..>3125)
                     /locus_tag="VF686_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660476.1"
                     /translation="DGTVDYMNDRGLELLEIPSFEANRGKVWADLWPEETRDALQAAM
                     QEAMEGHPAEFEGYCPTATGKPRWWRTIISPVRNETGAVIRLLAQSRDVTAERRRDAR
                     RLATVRAARRAATAKATFLNYLRKALEVMPAGLAFYDADDRLVIWNQQYVRAGAGDLE
                     AVHLREGMSFRELLESDLAKGRHPDAVGREAEWLTERLAVRARGRDPEVQQLSNGQWY
                     RFEDRRLPDGGIVSVAVDITELKSREAALTETGLSLERAKEAAEAASQSKSAFLANMS
                     HEIRTPLNGVVGMADMLCRARLAPAQHELAEIIRSSGETLERLLSDILDLSRIESGSV
                     TIERAGFHLGDAIRTVVALSQLKAQEKGLSLEFAIDPTLDRRVDGDAMRVRQILTNLI
                     SNAIKFTEAGRIVLRVAPLESGMVRFSVEDTGVGFDMAEKNRLFRRFEQADASITRQY
                     GGSGLGLAICQELASLMGGAVDCTSTPGVGTTFWADLRLDAVEAIEAAAPDAPPQAAM
                     EGGLRVLAADDHPVNRKVIEMILQEAGAEVTSAENGAEAVEAFRNGPFDLILMDMQMP
                     VLDGLSAIREIRRIEQDEQRPPTLTMVLSANTLPDHVRASIEAGADGHMAKPITAARL
                     LSAVEEAVGLASADPTVRSA"
BASE COUNT          438 a         1165 c         1034 g          488 t
ORIGIN      
        1 gcgccgacgg ttcagccgtt cgcccggcct cgggcggcgc ggccttggcc ggagcttcga
       61 ccctgttctt cggcgcgggc gtcgccgggt cgcaagcggc cgcggccaga aggagcagaa
      121 acacagatcg cgatcgtctc atgtccggaa ctccacatag ggcagacgag agcggaacaa
      181 cccggctccc caacggttcc tgtcggcgtc ttcagcccgg cgtgtacgcc ggatgaaccc
      241 atgaccgatc ccctcgccgc cctcgacacc cccccgcgtc gtcgtgtctg acggggaaat
      301 cgttctgctc ggcgaccggg tcagcgtcgc ctatccgccg gccgccgcgc gtcggctcgc
      361 cgacgatctg cacctcgtcc tgaccggcct gggcctctag cgtcctgtcc gctgaccgac
      421 ggtcacggac ccgccacttt cccagcctct cttctccccg gggcggattg cgagacgggt
      481 catgaagcgt atcggactgg tatttctctt gctggcggcg ggttgcaccc agccgccgga
      541 tccgcgcggc gtgcgcccca atgaggtgct gcttcaggtc gccgccaccg gacgcgccga
      601 ggcccggccc gacgaagccc ggctgacggt gggcgtgtcg accaacgccc cctcggcggc
      661 cgccgccagc gccggaaaca acgccgccat ggcgcgcgtc tcggccgccc tcgcacgtct
      721 gggcgtcaag cccgacgaca tccagacccg ctccgtcacc ctcggccgcg tcgaatacgg
      781 ccccggccgc ggcggctacc gcgccgaaaa catggtcgag gtgcggattc gcgacgtccg
      841 tcaggcgggc cccgccatct ccgccgcgac cgaggccgga ggcaacgtcg tgtcgggccc
      901 cgacctgcag gcttcggatc ccgagcgggc gaagaacgcc gcctacgccg ccgcctacaa
      961 ggccgcccgc gcccgcgccg aggcctacgc cgcagccgcc ggcctcaagg tcgaacgggt
     1021 catcgccatc cgcgacggct cgggccagcc gccggctccc tggtatatgg gcgacgcgcg
     1081 cggcgcggtg gcccagtcgg tcgccccggc gccgccggtc cacgccggcg ccagcaccta
     1141 tgaggtgcag gtccacgccg acttcgccct ggcgcggtag gtccgccggt tcaggcggag
     1201 cggactgtcg ggtcagcgga ggccagaccc acggcctctt cgacggcgct cagcaaccgc
     1261 gccgccgtga tcggtttggc catgtggccg tccgcgcccg cctcgatgga ggcccgcacg
     1321 tgatcgggca gggtgttggc ggacagcacc atcgtcagcg tcggcggccg ctgctcgtcc
     1381 tgctcgatcc gacgaatttc ccggatggcg gacagtccgt ccagaacggg catctgcatg
     1441 tccatcagga tcaggtcgaa ggggccattg cgaaacgcct ccacggcctc cgcgccgttc
     1501 tcggccgagg tgacctccgc ccccgcctcc tgcaggatca tctcgatcac cttgcggttc
     1561 accgggtggt cgtcggcggc cagtacgcgc aggccgccct ccattgcggc ctgcggaggc
     1621 gcatcgggcg ccgcagcctc gatcgcctcc accgcgtcga ggcgcagatc ggcccagaag
     1681 gtggtgccga cgcccggcgt gctggtgcaa tcgacggcgc cgcccatcag gctcgccagt
     1741 tcctggcaga tggccaggcc caggccgctg ccgccatact gccgcgtgat cgaggcgtcg
     1801 gcctgttcga agcggcggaa gagccggttc ttctcggcca tgtcgaagcc cacgcccgtg
     1861 tcctcgacgc tgaaccgcac catgcccgac tcgagcggcg ccacgcgcag gacgatccgt
     1921 ccggcttcgg tgaatttgat cgcgttcgag atcaggttgg tcagaatctg gcggacgcgc
     1981 atggcgtcgc cgtcgacccg ccggtcgagc gtcggatcga tcgcgaattc caggctgagc
     2041 cccttctcct gggccttcag ctgcgacagg gccacgaccg tccgtatcgc gtcgcccaga
     2101 tggaaaccgg cccgctcgat ggtgacgctt cccgattcga tccgcgacag gtccaggatg
     2161 tccgacagca gccgctccag cgtctcgccc gagctgcgga tgatctcggc cagctcgtgc
     2221 tgggcgggcg ccagacgggc gcggcacagc atatcggcca tgccgaccac gccgttcagg
     2281 ggcgtccgga tctcgtggct catattggcc aggaaggcgc tcttggactg gctcgcggcc
     2341 tcggcggcct ccttcgccct ttcaaggctg aggcccgtct cggtcagggc ggcctcgcgc
     2401 gatttcagct cggtgatatc gacggcgacc gagacgatgc ctccgtccgg caaccggcgg
     2461 tcctcgaatc ggtaccattg gccgttggac agctgctgca cctccggatc gcgtccgcgg
     2521 gcgcgaaccg caagccgttc ggtcagccat tccgcctcac gtccgacggc gtccggatgg
     2581 cggcccttgg cgaggtccga ttccagcagc tcgcggaagc tcatgccctc gcgcaggtgc
     2641 acggcctcca gatccccggc gccggccctg acgtattgct ggttccagat caccagccga
     2701 tcgtcggcgt cgtagaaggc caggccggcc ggcatcacct ccagcgcctt gcgcagatag
     2761 ttcaggaagg tggccttggc cgtcgcggcg cggcgcgcgg cgcgaaccgt cgccagacgc
     2821 cgcgcgtcac gccggcgttc ggcggtaacg tcgcgggact gggccagcaa ccggatcacc
     2881 gcgcccgtct cgtttcgcac cggcgagatg atcgtccgcc accagcgcgg cttgccggtc
     2941 gccgtcggac aatagccctc gaactcggcc ggatggccct ccatggcctc ctgcatggcc
     3001 gcttgcaggg cgtcgcgggt ctcttcgggc cagagatccg cccagacctt gccgcggttt
     3061 gcctcgaacg acggaatctc gagcagttcg agaccccgat cgttcatgta gtcgaccgta
     3121 ccgtc
//
LOCUS       DASVVN010000084         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_186627,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000084 DASVVN010000000
VERSION     DASVVN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186627"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VF686_01325"
     CDS             complement(<1..509)
                     /locus_tag="VF686_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEX8660477.1"
                     /translation="MTPGATGTGRATGPSRLATYFHVHLVSDSTGETLNAMAKAVVAR
                     FDGVIPIEHIYALVRNDKQMDRVLEEIASSPGVVLHTLVDRELREQLEAGCRRLDMPQ
                     IAALDPLVGALSRYLGAALSTRVGAQHALDTDYFNRIAALDFAMAHDDGQGTTAQLES
                     ADVVLCGVSR"
     gene            995..2041
                     /gene="hemE"
                     /locus_tag="VF686_01330"
     CDS             995..2041
                     /gene="hemE"
                     /locus_tag="VF686_01330"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX8660478.1"
                     /translation="MNASPPTPLLLKVLAGETTSRPPVWFMRQAGRYLPEYRALRATT
                     PDFISFCLDPDKAAEATLQPMRRFGFDAAIVFADILLIPRALGQEVWFEAGEGPRLGA
                     LPPVEAMRELTSGAGEALKQVGETLSIVRSQLEPERALIGFAGAPWTVATYMLDGEAR
                     TIGKGERAQARTYAYAEPEKVAALLDVLVEATAHYLKMQADAGAQVLKIFESWAEGLP
                     EDLFESLVLRPHQQLVRRTRELGVTVPLIGFPRGSAALAERYAAEVDVQAVALDTACP
                     LEIGRRIQAIKPIQGALDPLLLRAGGPMLDRRVDQLLEAWGQGPWIFNLGHGILPDVP
                     IAHVEQVLKRIGAQ"
     gene            2038..3111
                     /gene="hemH"
                     /locus_tag="VF686_01335"
     CDS             2038..3111
                     /gene="hemH"
                     /locus_tag="VF686_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX8660479.1"
                     /translation="MTEPGTGAAAGRRIAVVLFNLGGPDDQASVRPFLFNLFRDPAII
                     GLPNPFRTWLAGLISSRRETSAQANYALMGGGSPLLAETRKQAAALEQALATRLPGDT
                     VRTFIAMRYWSPLTEQTAAEVTIFGPDETILLPLYPQFSTTTTESSLKAWREAYAGPG
                     VSRTVCCYPQAPGWIETQAAAVRAARLRSGDQPVRVLFSAHGIPESLIAKKGDPYQEQ
                     IEATCAAIAAAAGLAERETDWTICYQSRVGPMKWLGPSTPEAIQQAAGDGVGVIVTPV
                     AFVSEHIETLVELDIEYAELAHGLGVAPYLRTPAVGVAAPFIQTLSDAVLEALPRGGV
                     APYGPGCRAAWKACPAQCTRKAA"
     gene            3111..3605
                     /locus_tag="VF686_01340"
     CDS             3111..3605
                     /locus_tag="VF686_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX8660480.1"
                     /translation="MLDVTTFEIVRGLHILAVIAWMAGMLFLPRLYAYDAEQADKTEP
                     LRSEMQSLLRTWQKRLLKIIINPAMTLAWVFGVWMILIHVYGFGEGWGFLAQPWMETK
                     LLAVVALSGWHGFLSGELKRIAAGTSIRTGRFWRMTNEIPFVIAIVAVLSVTTQWTFG
                     PGPA"
     gene            3806..>4668
                     /gene="rho"
                     /locus_tag="VF686_01345"
     CDS             3806..>4668
                     /gene="rho"
                     /locus_tag="VF686_01345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX8660481.1"
                     /translation="MTDVRDTDPTAPEAENLEGEQPSTEIAAADLPMVDQEQGDDEDD
                     GEPIVPNGRITLQELNDKTPEELVAFAESLEIENAGNLRKQDLLFAILKTLADEEVEI
                     IASGTLEILPDGFGFLRSPDVNYLPGPDDVYVSPSQIRRFGLRSGDTVHGAVRGPREG
                     ERYFALLKVDTINFEDPETVKTKVLFDNLTPLYPEERLHMEIQDPTLKDRSGRVIDIV
                     APLGKGQRCLIVAPPRVGKTVMLQNIAKSIERNHPEVTLMVLLIDERPEEVTDMQRTV
                     KGEVIASTFDEP"
BASE COUNT          754 a         1652 c         1514 g          748 t
ORIGIN      
        1 cggctgaccc cgcacagcac cacatcggcg ctttccagct gggccgtcgt accctggccg
       61 tcgtcgtggg ccatggcgaa gtccagcgca gcgatgcggt tgaaatagtc cgtgtccagc
      121 gcgtgctgcg cccccacccg cgtcgacagg gctgcgccca gatagcgcga cagggccccg
      181 accagcgggt ccagggccgc gatctggggc atgtccagcc gacggcagcc cgcctccagc
      241 tgctcgcgca gctcccggtc gaccagggta tgcagaacca cgccgggcga ggaggcgatc
      301 tcttccagca cccggtccat ctgtttatcg ttccgcacca gggcgtagat gtgctcgatc
      361 ggaatgaccc cgtcgaaacg ggcgaccacg gccttggcca tggcgttcag cgtctcgccg
      421 gtggaatccg acaccagatg gacgtggaaa taggtcgcca gccgcgaggg gcctgtcgcg
      481 cgtcccgtcc ctgttgcacc cggcgtcatc ggcggctgtc catatgtgcg agactcctgt
      541 ggaccgggcg gggcgccgtt ggccatcggc tgtcggtctt ttcctatagc cgggccggac
      601 gggggcggcg agcggggaca gaggtggacg gcgcaggcgc cggggaggcg gttcatcccg
      661 aggccgcggt ccgtatccgg atcgcctgtg gagatcctta atgagacgtt aatggcgccc
      721 agcctcccca aggctttccc ggcgtcaaac cccgcccttg aagcctgcct ggattttatc
      781 cccgctttca ccagcccggt gcacgccggt gcgcagtctg tgaccggttg cggaaaaccc
      841 gtccgcccgc gtccgaaaga ccgcgcttat ccccgccatc ccccgccctg tcttctacct
      901 cccattcttc gaatcttttc ttgataagga ttggaggggt gcgacacggg ccggggttga
      961 gcctgaaccg ggccgccggt aggaccgggg tcacatgaac gcctcacccc ccacgccatt
     1021 gctcctcaag gttctcgccg gagagaccac gtcacgtccg ccggtctggt tcatgcgtca
     1081 ggccgggcgc tacctgccag agtaccgggc gctcagggcg acgacgccgg acttcatctc
     1141 cttctgcctg gatcccgaca aggccgccga ggcgaccctg cagccgatgc ggcggttcgg
     1201 cttcgacgcg gccatcgtct tcgccgacat cctgctgatc ccccgggcgt tggggcagga
     1261 ggtctggttc gaggccggcg agggaccgcg gctcggcgcc ctgccaccgg tcgaggccat
     1321 gcgggagctg acctccggcg ccggcgaggc cctgaaacag gtcggcgaga ccctgtcgat
     1381 cgtgcggagc cagctggagc cggaacgcgc cctgatcggt ttcgccggcg cgccctggac
     1441 cgtcgccacc tacatgctgg acggcgaggc ccggaccatc ggcaagggcg aacgcgccca
     1501 ggcccggacc tacgcctacg ccgagccgga aaaagtcgcg gcccttctgg acgtgctggt
     1561 cgaggccacg gcccattatc tgaagatgca ggccgacgcc ggggcccagg tgctgaagat
     1621 cttcgagagc tgggccgagg gcctgcccga ggacctgttc gaaagccttg tcctgcggcc
     1681 tcatcagcag ctggtgcgac ggacgcgcga actgggcgtc accgtgcccc tgatcggctt
     1741 cccgcggggc tcggcggcgt tggcggaacg ttatgcggca gaggtcgacg ttcaggccgt
     1801 ggcgctggac accgcctgcc cgctggagat cggccgccgc attcaggcga tcaagccgat
     1861 ccagggcgcg ctggatcccc tgctgctgcg cgccggcggg ccgatgctgg accgccgcgt
     1921 tgaccagctg ctcgaggcct gggggcaggg gccgtggatc ttcaacctcg gccacggaat
     1981 cctgcccgac gtgccgatcg cccatgtcga acaggtgctg aagcggatcg gcgcgcaatg
     2041 acagagccgg ggacgggcgc ggccgccgga cgacggatcg cggtggtcct gttcaatctc
     2101 ggcgggcccg acgatcaggc cagcgtgcgg cccttcctgt tcaacctgtt ccgcgacccg
     2161 gccatcatcg gcctcccgaa cccgttccgg acctggctgg ccgggctgat ctccagccgg
     2221 cgcgaaacca gcgcacaggc gaactacgcc ctcatgggcg gcggctcgcc tctgctggcc
     2281 gagacccgca aacaggcagc ggcgctggaa caggcgctgg cgacgcggct gccgggtgat
     2341 accgtccgca ccttcatcgc catgcgctac tggtcgcccc tgaccgaaca gaccgccgcc
     2401 gaggtcacga tcttcgggcc ggacgagacc atcctcctgc ccctctatcc gcagttctca
     2461 acgaccacga cggagtcctc gctcaaggcg tggcgcgagg cctacgccgg tcccggcgtc
     2521 agccgcaccg tctgctgcta tccgcaggcc ccgggctgga tcgagaccca ggcggcggcg
     2581 gtgcgcgccg cccgactcag gtccggagac cagccggtcc gcgtcctgtt ctcagcccac
     2641 ggcattccgg aaagcctgat cgcgaagaag ggcgatccct atcaggagca gatcgaggcc
     2701 acctgcgccg ccatcgccgc cgcggccgga ctggccgagc gcgaaaccga ctggaccatc
     2761 tgctaccaga gccgggtcgg gccgatgaaa tggctgggcc cgtcgacgcc cgaggcgatc
     2821 cagcaggcgg ccggtgatgg cgtcggggtg attgtgacac ccgtcgcctt cgtctccgaa
     2881 cacatcgaga ccctggtaga gctcgacatt gagtacgccg aactggccca cggtctgggc
     2941 gtcgcccctt atctgcgcac ccccgcggtc ggcgtggccg cccccttcat ccagaccctg
     3001 tccgatgcgg ttcttgaggc cttgccgcgc gggggcgtcg cgccctacgg tcccggttgc
     3061 cgggcggcgt ggaaggcctg cccggcccaa tgcacaagga aggcggcctg atgctggatg
     3121 tcacgacatt cgagatcgtc cggggtctgc atattctggc cgtcatcgcc tggatggcgg
     3181 gcatgctctt cctgccgcgt ctctatgcct atgacgccga acaggccgac aagaccgaac
     3241 cgctgcgctc ggagatgcag tccctgctgc ggacctggca gaaacgcctg ctgaagatca
     3301 tcatcaatcc ggccatgacc ctcgcctggg tgttcggcgt ctggatgatc ctgatccacg
     3361 tctacggctt cggcgagggc tggggcttcc tcgcccagcc gtggatggag accaagctgc
     3421 tggccgttgt cgccctcagc ggctggcacg gcttcctgtc cggcgagctg aaacgcattg
     3481 cggcggggac ctcgatccgg accggccgct tctggcggat gaccaacgaa atcccgttcg
     3541 tgatcgccat tgtggcggtg ctgtcagtca cgacccaatg gacctttggg ccaggtcccg
     3601 cttgacctga ccgggctggc gtggttcccc tcatcccgaa ccgttcctgg cagcgccggg
     3661 cggtccctct ctcccagcga cgccgatcgg atctctcccg tcggaacccg tcgttctccc
     3721 tgccggccgc aaggccagac atcgtcagcc cgcccgacct cgccgtttcg cgagcgcccg
     3781 gccgcgcatg ttcaagaagc ctcccatgac cgacgtccgc gacaccgatc ccaccgcccc
     3841 cgaagctgaa aacctcgaag gcgaacagcc gtcgacggag atcgccgccg ccgacctgcc
     3901 catggtcgat caggagcagg gtgacgacga ggacgacggc gagccgatcg tccccaacgg
     3961 ccgcatcacc ctgcaggagc tcaacgacaa gacgccggaa gaactggtcg ccttcgccga
     4021 gagcctcgag atcgagaacg ccggcaacct gcgcaaacag gacctgcttt tcgctatcct
     4081 gaagaccctg gccgacgaag aagtcgagat catcgcttcg gggacgctgg aaatcctgcc
     4141 cgacggtttc ggcttcctgc gcagcccgga cgtcaactat cttccgggac cggacgatgt
     4201 ttacgtctcc ccctcacaga tccgccgctt cggcctgcgc tcgggcgata ccgtccacgg
     4261 cgccgtccgc ggcccgcggg agggcgagcg ctacttcgcc ctgctcaagg tcgacacgat
     4321 caatttcgag gatccggaaa cggtcaagac caaggtcctg ttcgacaacc tgaccccgct
     4381 ctatcccgaa gagcggctgc acatggaaat ccaggacccg acgctgaagg accgctcggg
     4441 ccgggtcatt gacatcgtcg ccccgctcgg caagggccag cgctgcctga tcgtcgcccc
     4501 gccgcgcgtc ggcaagacgg tgatgctgca gaacatcgcc aagtcgatcg agcgcaacca
     4561 ccccgaagtg acgctgatgg tgctgctgat cgacgagcgc ccggaagaag tcaccgacat
     4621 gcagcgcacg gtgaagggcg aggtcatcgc ctcgaccttc gacgagcc
//
LOCUS       DASVVN010000085         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_266056,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000085 DASVVN010000000
VERSION     DASVVN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266056"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="rpsB"
                     /locus_tag="VF686_01350"
     CDS             complement(<1..598)
                     /gene="rpsB"
                     /locus_tag="VF686_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX8660482.1"
                     /translation="MALPEFSMRSLLEAGAHFGHQTHRWNPKMERYIFGSRSNIHIID
                     LSQSIPLLHQALVAVRDVAAKGGRVLFVGTKRQAADPVAEAAKRCAQYYMNNRWLGGT
                     LTNWRTVSGSIARLRELEGLLESGGEGRVKKELLNLQREKDKLELSLGGIKDMGSIPD
                     IMFVIDTNKEAIAILEARKLNIPIIAILDTNSDPDGITY"
     gene            complement(755..1960)
                     /gene="chrA"
                     /locus_tag="VF686_01355"
     CDS             complement(755..1960)
                     /gene="chrA"
                     /locus_tag="VF686_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX8660483.1"
                     /translation="MAEDVDHRPPNLSYPALFLRFLRFGVLAFGGPVAQIAMVRRELV
                     EQERWISPARFNRLLAVMQALPGPEAHELCVHLGIQAKGRIGGLLAGLGFMLPGFVLM
                     LLAAWAYFRFDVDRGLAGAALIGAQAAVVALIVRAAHRIGEHVLIDRGLWTIGVAAGT
                     ATLLGAPFWVILPPGAAIYALVRSGRRGAAALLLVACLVLLAVWSAGQGGAVMEAPAG
                     GSFSGDGAWPLLFVSGLKAGLLTFGGAYTAIPFLRQDVVGRGWLGDGTFLDGVALSNV
                     LPAPLIIFATFAGYAVGGFAGAVAITAGVFLPAFAFSMIFYDRLEAVVSEPRIRSALD
                     GVAAAVVGLIAATAASLAVGLLRSPDPVASVAIAGVALAALYVVKSRYAPPVILLLAG
                     LAGLGLSLT"
BASE COUNT          406 a          729 c          729 g          295 t
ORIGIN      
        1 gataggtgat gccgtccggg tccgaattgg tgtccaagat ggcgatgatc gggatgttca
       61 gcttgcgggc ttcaaggatg gcgatggcct ccttgttggt gtcgatcacg aacatgatgt
      121 ccgggatgga gcccatgtcc ttgatgccgc ccagcgacag ctcgagcttg tccttctcgc
      181 gctgcaggtt cagcagctct ttcttgacgc ggccttcgcc acccgactcc agcaggcctt
      241 ccagctcgcg cagtcgggcg atggagcccg acacggtgcg ccagttagtc agggtgccgc
      301 cgagccagcg gttgttcata tagtactggg cgcagcgctt ggcggcttcg gccaccggat
      361 cagcagcctg acgcttggtg ccaacgaaca ggacgcggcc gcccttggcg gcgacgtcgc
      421 gaacggcgac cagggcctgg tgcagcagcg ggatcgactg cgacaggtcg atgatgtgga
      481 tgttcgagcg cgagccgaag atgtagcgct ccatcttcgg gttccaccgg tgcgtctggt
      541 ggccgaagtg ggcgccggct tcgagcagcg aacgcatgga gaattcaggc agagccatga
      601 ggggtagtcc tttatccgat tgatccgccg caggcgctgt aaagaccgaa gtcctcggaa
      661 cggagcgaac aggatttctc cctgaccgcg ccacgcctgc gtgtgaagtg ggcgggcatg
      721 taggcgggat ggccgcaaaa ggcaagcgcc gcggtcaggt caggctgagg cccagtccgg
      781 cgagtccggc gagcagaagt atgacgggcg gcgcataccg gcttttgacg acatacaggg
      841 ctgcgagggc cacgcccgcg atggcgacgg acgccacggg atccggcgag cgaagcaggc
      901 cgacagcgag gctggccgcg gtcgccgcga tcagtcctac gacggccgcc gcgacgccgt
      961 cgagggcgct acggatgcgc ggctcggaca cgacggcctc gagccggtcg tagaagatca
     1021 tggaaaacgc gaaggccggc aggaagacgc cggccgtgat ggccacggcg ccggcgaaac
     1081 cgcccacggc gtagccggcg aaggtcgcga agatgatcag gggcgccggc agcacgttcg
     1141 acagggcgac gccatccaga aaggtcccgt cgccgagcca gccgcgcccg acgacgtcct
     1201 ggcgcaggaa cgggatcgcc gtataggccc cgccgaaggt cagcaaacca gccttgagtc
     1261 cggacacgaa caggaggggc caggcgccat cgcctgaaaa cgatccgcca gcgggcgcct
     1321 ccataaccgc cccgccctgc ccggcgctcc agaccgcgag caggacgagg caagcgacca
     1381 gcaggagcgc cgccgctccg cgacgtccgc tccggacgag ggcgtagatc gccgcgcccg
     1441 gaggcaggat gacccagaat ggcgctccca gcagcgtcgc cgtcccggcc gccactccaa
     1501 tggtccacag tccccggtcg atcagcacat gctcgccgat ccggtgcgcg gcgcggacga
     1561 tcagggccac cacggcggcc tgcgccccga tcagggccgc tccggccagg ccgcgatcga
     1621 cgtcgaagcg gaaataggcc cacgccgcca acagcatcag cacaaagccc ggcagcatga
     1681 agcccagccc ggcgagcaag cccccgatgc gacccttcgc ctgaatgccc agatgcacgc
     1741 agagctcatg cgcttccggc ccgggtagcg cctgcatgac ggcgagcagc cgattgaacc
     1801 gcgcgggcga aatccaccgt tcctgctcga ccagttcacg ccggaccatc gcgatctgcg
     1861 cgaccgggcc gccgaacgcc agaacgccga acctcaggaa gcgcaggaac agcgccgggt
     1921 acgacagatt cggcggacgg tgatcgacat cctcggccat gtccggacga tgccagcttt
     1981 gccaaggctc gtcagccgcc ctctcgcaac tttcgcataa cgacgctatt gtcggtatcc
     2041 gggagggtcg atcacatgaa catccgcacc ggggtcttcg ccggaggcat aatcctggcc
     2101 gccgccatcg ccggcgccgc cacctcgcaa cagcaaccgc cgtcgcccgt ggccgcggc
//
LOCUS       DASVVN010000086         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_450458,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000086 DASVVN010000000
VERSION     DASVVN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450458"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="phbB"
                     /locus_tag="VF686_01360"
     CDS             complement(<1..694)
                     /gene="phbB"
                     /locus_tag="VF686_01360"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX8660484.1"
                     /translation="MARVALVTGGTRGIGAAISQGLKAAGYKVAANYAGNDEAANKFK
                     AETGIPVYKWSVAEYEACQAGIKQVEAEVGPVDILVNNAGITRDAMFHRMTPAQWREV
                     IDTNLNGVFNMTHPVWSGMRDRKFGRVITISSINGQKGQAGQANYSASKAGDIGLTKA
                     LAQEGARAGITVNVIAPGYIGTEMVKAIDEKVLNERIIPLIPVGRLGTPEEIARAGVF
                     LASEEGGFITGST"
     gene            complement(826..>1671)
                     /locus_tag="VF686_01365"
     CDS             complement(826..>1671)
                     /locus_tag="VF686_01365"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8660485.1"
                     /translation="IVAGGQESMSQAPHAAYMRSGAKMGDFKLVDTMMKDGLVDAFHG
                     YPMGNTAENVAQRWQLTREEQDQFATASQNKAEAAQKAGKFKDEIVAVTVPGRKGDTV
                     VDEDEYIRAGTTVDALAKLRPAFSKDGTVTAGNASGINDGAAALVLMTAENAKKRGLT
                     PLARIASWATAGVDPAIMGTGPIPSSRRALEKAGWKVGDLDLVEANEAFAAQACAVNK
                     DMGWDPEIVNVNGGAIAIGHPIGASGARVLVTLLHEMQKRNSKRGLATLCIGGGMGVA
                     MCLER"
BASE COUNT          211 a          563 c          578 g          319 t
ORIGIN      
        1 gggtcgagcc ggtgatgaac ccgccctcct cgctcgccag gaacacgccc gcgcgggcga
       61 tctcctccgg cgtgccgagg cggcccacgg ggatgagcgg gatgatgcgc tcgttcagca
      121 ccttctcgtc gatggccttc accatctcgg tgccgatgta gcccggcgcg atgacgttca
      181 cggtgatccc ggcccgcgcg ccctcctggg caagcgcctt ggtcaggccg atgtcgccgg
      241 ccttcgacgc ggagtagttc gcctggccgg cctggccctt ctgcccgttg atggacgaga
      301 tcgtgatgac gcggccgaac ttgcggtccc gcatgccgct ccagaccggg tgcgtcatgt
      361 tgaagacgcc gttgagattg gtgtcgatga cctcgcgcca ctgggcgggg gtcatgcggt
      421 gaaacatggc gtcgcgcgtg atgccggcgt tgttgaccag aatatccacc ggcccgacct
      481 cggcctcaac ttgcttgatg ccggcctggc aggcctcgta ctcggccacc gaccacttgt
      541 agaccgggat gccggtctcg gccttgaact tgttcgcggc ctcgtcgttg ccggcgtagt
      601 tcgccgcgac cttgtatccc gcagccttca ggccctgcga gatcgcggcg ccgatgccgc
      661 gggttccgcc cgtcaccaat gcaacccgtg ccatctcgtg tttcctccgt tttatgtcgt
      721 tttaagttgg gcactcggca ggtcggcagg tcggcagtgg ggagtccgca gttcggatgg
      781 gatgcccgcc ctactgccta ctgcctaccg ccgactgcgt tcagcctagc gttccaggca
      841 catggcgacg cccatgccgc cgccgatgca cagggtggcg aggccgcgct tggaattgcg
      901 cttctgcatc tcgtgcagga gcgtgacgag gacccgggcg cccgaggcgc cgatggggtg
      961 gccgatggcg atggcgccgc cgttgacgtt cacgatctcc gggtcccagc ccatgtcctt
     1021 gttgaccgcg caggcctgcg ccgcgaaggc ctcgttggcc tcgacgaggt cgaggtcgcc
     1081 gaccttccag ccggccttct cgagggcgcg gcgcgacgac gggatcgggc ccgtgcccat
     1141 gatggcggga tcgacgccgg ccgtcgccca ggaggcgatg cgggcgagcg gcgtgaggcc
     1201 gcgcttcttg gcgttctcgg ccgtcatcag cacgagggcg gcggcgccgt cgttgatgcc
     1261 cgacgcattg ccggccgtca cggtgccgtc ctttgagaag gccggccgga gcttggcgag
     1321 ggcgtcgacg gtcgtcccgg cccggatgta ctcgtcctcg tcgacgacgg tgtcgccctt
     1381 gcggcccggg accgtgaccg ccacgatctc gtccttgaac ttgccggcct tctgggcggc
     1441 ctcggccttg ttctgggagg cggtggcgaa ctggtcctgc tcctcgcggg tgagctgcca
     1501 gcgctgggcc acgttctcgg cggtgttgcc catcgggtag ccgtggaagg cgtccaccag
     1561 gccgtccttc atcatggtgt cgacgagctt gaaatcgccc atcttggcgc cgctgcgcat
     1621 ataggctgcg tgcggagcct gggacatgga ttcctggccg ccggccacga t
//
LOCUS       DASVVN010000087         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_109123,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000087 DASVVN010000000
VERSION     DASVVN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109123"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF686_01370"
     CDS             <1..313
                     /locus_tag="VF686_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX8660486.1"
                     /translation="VLDGTGKVVWSGDAPAKTTGMHEFTWDGEATTGNDGQDGQVYSL
                     KIVAKNAAGGAVDAQVLTRGRISGVEMYDGEAYLTIGKSIVPLSTIIALEEPEDPAVA
                     A"
     gene            365..>1633
                     /locus_tag="VF686_01375"
     CDS             365..>1633
                     /locus_tag="VF686_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX8660487.1"
                     /translation="MSINSALLAGVSGLTANSAALAAISQNIANVNTVGYKRTAGEFQ
                     TVINSQSQGAGYSAGGVLSTARHYTSQAGQLQRTTSATDLGIAGQGFFVVTEKPENLQ
                     VTDSRLFTRAGAFTVDNLGYLKNTAGLYLQGWPVDAEGNIATDPSDLNRLRTINVGSV
                     GGTAEATTRIQLNANIKSSQLVSAEATDASLTPPGANAYNRTTNSMAMWDPETGAGVK
                     PDFELTIPVSDSKGGQRTIAISFLKSTVPNQWYSEIRAIPASDVVTGAGLTNGQLKTG
                     LVAFTQDGRLDVDAMAAMGAAALFPDPSAASLTFGASDSAAPAAGGFKWAPGLGIDDQ
                     SLAFDLSASAGGLTQYDSASVVQAVLTNGTAFGNLSEVKIDDTGFVTAIFDNGVTRQI
                     AQIALATFPSPDSLSQTSGNAYRVSQGSGTY"
BASE COUNT          291 a          618 c          487 g          237 t
ORIGIN      
        1 ggtgctggac ggaaccggca aggtcgtctg gtccggcgac gcgcccgcca agacgaccgg
       61 catgcatgaa ttcacctggg acggcgaggc caccaccggc aacgacggcc aggacggcca
      121 ggtctattcg ctgaagatcg tcgccaagaa cgccgccggc ggcgccgtgg acgcccaggt
      181 cctgacccgc ggccgcatca gcggcgtcga gatgtacgac ggcgaggcct acctgacgat
      241 cggcaagtcg atcgtgccac tctccaccat catcgccctc gaagaacccg aggatccggc
      301 cgtcgcggcc tgatttgccc cgcaatgacc ctttcaaacc gtttctcgta aggagccccg
      361 cgccatgagt atcaacagcg cccttctggc cggcgtgtcc ggcctgaccg caaactccgc
      421 cgccctcgcg gcgatctcgc agaacatcgc gaacgtgaac accgtcggct acaaacgcac
      481 cgccggcgag ttccagaccg tgatcaacag ccagtcccag ggcgccggct attccgccgg
      541 cggcgtcctg tccacggcgc gccactacac cagccaggcc ggacagctgc agcggaccac
      601 ctccgccacc gatctgggca tcgcgggtca gggcttcttc gtcgtcaccg agaagccgga
      661 gaacctgcag gtcaccgaca gccgcctgtt cacccgggcc ggcgccttca ccgtcgacaa
      721 cctcggctat ctgaagaaca ccgccggcct gtacctgcag ggctggccgg tggatgcgga
      781 gggcaatatc gccaccgatc cctccgacct gaaccgcctg cgcaccatca acgtcggctc
      841 ggtcggcggc acggcggaag ccacgacccg catccagttg aacgccaata tcaagtccag
      901 ccagctggtc tcggccgaag cgaccgacgc ctcgctcacc cctccgggcg ccaacgccta
      961 caatcgcacg accaattcga tggccatgtg ggacccggaa accggcgccg gcgtgaagcc
     1021 cgacttcgaa ctgaccatcc ccgtttccga ctccaagggc ggccagcgca ccatcgccat
     1081 ctccttcctg aagagcacgg tgccgaacca gtggtattcc gagatccgcg cgatcccggc
     1141 gtccgacgtc gtcaccggcg cgggcctgac caacggccag ctgaagaccg gtctggtggc
     1201 cttcacccag gacggacgtc tggatgtgga cgccatggcc gccatgggcg cggccgcctt
     1261 gttccccgat ccctccgccg cctccctgac gttcggagcg tcggacagcg cggctccggc
     1321 ggccggcggc ttcaaatggg cgccgggtct gggcatcgac gatcagtcgc tggccttcga
     1381 cctgtcggcg tcggccggcg gcctgacgca gtatgacagc gcctcggtcg ttcaggccgt
     1441 gctgaccaac ggcaccgcct tcggcaacct gtccgaggtc aagatcgacg acaccggctt
     1501 cgtgaccgcc atcttcgaca acggcgtcac gcgccagatc gcccagatcg ccctggccac
     1561 cttccccagc ccggacagcc tgtcccagac ctccggcaac gcctaccgcg tcagccaggg
     1621 gtcaggcacc tac
//
LOCUS       DASVVN010000088         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_450501,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000088 DASVVN010000000
VERSION     DASVVN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450501"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VF686_01380"
     CDS             complement(<1..580)
                     /locus_tag="VF686_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8660488.1"
                     /translation="MRGTTSLLSRLGRYRRDAQGVVALEFAFLAIPFLFMIMALLELA
                     LVFLVSAALDTAMERAARTIRTGGLQTSAAFAGLSGTALEDAQETAFETQVCREMGWL
                     VTGCRNALYVDVRTFADWAATNTCDPIRPPAGGGGPTFDEDAILFRAGGPRDIVLVRG
                     YYRWPLITPFVSQALVDLQGGVKLITSTQTFRN"
     gene            complement(659..1036)
                     /locus_tag="VF686_01385"
     CDS             complement(659..1036)
                     /locus_tag="VF686_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX8660489.1"
                     /translation="MRRLTLLLAAFAAAPALAAAATAGFEVPLDQAVRVPVRGDAVEV
                     VVGSPKYLDVTVIDPSTVLVHGKELGVTNLVIYDGAGRPVFNRQVAVVASNGAQVSLF
                     KGSKESAEYVCGDRCRATSRSGQ"
     gene            1347..>1528
                     /locus_tag="VF686_01390"
     CDS             1347..>1528
                     /locus_tag="VF686_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8660490.1"
                     /translation="MSKFVTRFMQDESGATAIEYGLIAALISITIIAAANAIGGTVRG
                     KFQNASDCMSGKLAAT"
BASE COUNT          266 a          474 c          557 g          231 t
ORIGIN      
        1 cgttgcggaa ggtctgcgtc gaagtgatca gcttcacccc gccctgcagg tcgaccagcg
       61 cctggctgac gaagggcgtg atcagcggcc agcggtagta gccgcgcacc agcacgatgt
      121 cgcgggggcc gccggcgcgg aacaggatgg cgtcctcgtc gaaggtcggc ccgccgccgc
      181 cggcgggcgg ccggatcggg tcgcaggtgt tggtggccgc ccagtcggcg aaggtgcgca
      241 cgtcgacgta cagggcgttg cggcagccgg tgaccagcca ccccatctcg cggcagacct
      301 gggtctcgaa ggcggtctcc tgcgcatcct cgagcgccgt gccgctgagg ccggcgaagg
      361 cggcgctggt ctgcagcccg cccgtgcgga tggtgcgcgc ggcgcgctcc atggcggtgt
      421 ccagcgcggc ggagaccagg aagaccagcg ccagctcgag cagcgccatg atcatgaaca
      481 ggaaggggat ggcgaggaag gcgaactcca gcgccacgac gccctgagcg tcgcggcggt
      541 agcgcccgag ccggctgagg agggacgtgg tcccgcgcat ggcttccccg atccgagtgg
      601 atctcgagaa gcaggctagc gcgccagccc taagaaaccg ttcgttagcg gccgtttcct
      661 actggccgga gcgggaggtc gcgcggcagc ggtcgccgca gacgtactcg gcgctctcct
      721 tcgacccctt gaacagcgag acctgggcgc cgttggaggc gaccacggcc acctgccggt
      781 tgaacaccgg ccggccagcg ccgtcgtaga tcaccaggtt ggtcaccccg agctccttgc
      841 cgtgcaccag cacggtggag gggtcgatca ccgtcacgtc gaggtacttg gggctgccca
      901 ccaccacctc gacggcgtcg ccgcggacgg ggacgcgaac cgcctggtcg agcgggacct
      961 cgaagccggc cgtggcggcg gcggcgaggg cgggagcggc ggcgaaggcg gccagcaaaa
     1021 gggtcagtcg gcgcatgggg ttcatccggc tacggacagg gttgctgcaa tgtgaacgac
     1081 aggggtcaag aattgctgaa cggccgggcg gggcgtctgc ccggggctcg cgcgctagag
     1141 agtctaatgt gcgggagggg ccggatcatg gtcgtggtta acaaatgtac atcgtgaata
     1201 cttgtaaacc acgatttatt tgcatggggc gccgccgttt tactggccat taagcgagat
     1261 aggcgaaatt ggcaggtgta gtaaggggct gagcgagcag agaaggctcg gtccccagtt
     1321 cgctcagtct gaacgggaga agacccatgt ccaagttcgt cacgcgtttc atgcaggacg
     1381 agtcgggcgc cacggccatc gaatatggcc tgatcgccgc cctgatctcg atcaccatca
     1441 tcgcggccgc caacgctatc ggcggcacgg tgcgcggcaa gttccagaat gcttccgact
     1501 gtatgtcggg caagctggcc gcgactga
//
LOCUS       DASVVN010000089         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_29204, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000089 DASVVN010000000
VERSION     DASVVN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29204"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VF686_01395"
     CDS             <1..1362
                     /locus_tag="VF686_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660491.1"
                     /translation="SAVITPYLVYAAGLPVFAAVYGLPILGVVNLAIACVIILVVSLM
                     VWRLSGQLLDAEESALEDATARRNEADAANAAKSAFVSTVSHELRTPMSAILAGAAAL
                     QERCEGSARNHAALIVDAGAMMQTLLDDLLDHAKIEAGRLDIEVLPMDARALVAKTVG
                     FWRSTARRKGLRLRVEGARDLPRWVLGDPTRLRQILNNLLSNALKFTDRGVVTLRIKT
                     RETGVLFEIQDTGPGLTPDQIARLFQPFEQGAAGRARTHGGTGLGLSISRNLARLMGG
                     DLTVESRAGDGATFRLATVLTACSAPPPEETEASDPAPQQTLRTLIVDDHEINQRAFG
                     VLLEAIGAQVSVADSGEAALRLLAVESFDLVLMDVNMAGLSGIETTRRIRAGDGCNRE
                     VPIIAVTGSVEPGDVALYLAAGMDRVVPKPVTPATLFRAIEDVLSPGLVSDPAPSPLP
                     SSG"
     gene            complement(1613..>2287)
                     /locus_tag="VF686_01400"
     CDS             complement(1613..>2287)
                     /locus_tag="VF686_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660492.1"
                     /translation="LEIELALSALPPHLRAEATVYVLDPNEGFERVREGTNGFHALVA
                     RNGDDAFRGAWMPTDYRDDIIYPVAFDSAGADAILPVFFDAAEMQADGVPASAVKALI
                     QERYATGYYKAPARAGISYMLSPVFRSYTAPEDNDQVGTVNYPHIMYYAPNVSARDVG
                     GTQPGSPYPFLIHPGPHAYNIQGLGDPDRRAINAEHTELIERLCSLRAEWCLPGDAPG
                     AAEHNH"
BASE COUNT          366 a          762 c          778 g          381 t
ORIGIN      
        1 tcagccgtga tcacgcccta tcttgtttac gcggcaggcc tgcctgtttt cgcagcggtc
       61 tacggcctgc ccatcctcgg cgtcgtgaac ctggcgatcg cctgcgtcat cattctcgtc
      121 gtgagcctta tggtgtggcg cctgtccggc cagctgctgg acgcggagga gagcgccctg
      181 gaggacgcca cggcgcggcg gaacgaggcc gacgcagcga acgccgccaa gtccgccttc
      241 gtatcgacag tgagtcacga attgaggacg cccatgagcg cgatcctcgc cggcgccgcc
      301 gcgttgcagg agcggtgcga gggctccgca aggaaccacg ccgccctgat cgtcgacgcg
      361 ggcgccatga tgcagacctt gctcgacgat ctgctggatc acgccaagat cgaggccgga
      421 agactcgata tcgaagtcct gccgatggac gcccgcgccc tggtggccaa aaccgtaggc
      481 ttctggcgct cgacggcccg gcgcaagggt ctgcgattgc gggtcgaagg cgcccgcgac
      541 ctgccgcgct gggtgctggg cgatcccacg cgcctgcgcc agatactcaa caatctgctc
      601 tcgaacgcgc tgaaattcac ggatcggggc gtcgtgacgc tgcgcatcaa aacccgggaa
      661 acaggcgttc tgttcgagat tcaggatacc ggcccaggtc tgacgcccga tcagatcgcg
      721 cgcctgtttc aacccttcga gcaaggcgcc gcgggccggg cccgcacgca cggcggcacg
      781 gggctcggcc tgtcgatcag ccggaacctg gctcgtctga tgggcggcga tctcaccgtg
      841 gaaagccggg ccggggatgg ggccaccttc aggctcgcca ccgtactgac cgcctgctcg
      901 gccccgcccc ctgaagagac ggaggcgtcc gacccggcgc cgcagcagac gctccggacc
      961 ctcatcgtcg acgatcacga gatcaaccag cgggcgttcg gcgttcttct ggaagcgatc
     1021 ggggcgcagg taagcgtcgc cgacagcggc gaagccgccc tgcggctgct cgccgtcgag
     1081 tcgttcgacc tggtgctgat ggacgtcaac atggcgggtc tgtccgggat cgagaccacc
     1141 cggcgaatac gcgccggcga cggctgcaac cgggaggtcc cgatcatcgc cgtaacaggt
     1201 tcggtagagc ccggggatgt tgctctctac ctcgccgccg gcatggatcg cgtcgttccg
     1261 aagcccgtca cgccggccac cctcttcaga gcgatcgaag acgttttgag ccccggcctc
     1321 gtgagcgatc cagccccgtc cccgctgccg tcgtcagggt gacggacagt agacggcgcg
     1381 gccggccggc ggcgaagacc gggatgcggc tcgtgacccg ttccgacctg cgcggatggc
     1441 gcgggtcggg acggttatga gatagccggc gagcgttctt tcgcgtctgc ccggacgacg
     1501 gtcgaacgcc ggtcctttcc agcgacgacc gcccgaacca tgcggaacga tccgtcgcga
     1561 gatccgaccg cgcccgtgtt cccgagggca tggagaccag ggtccgcccg cctcagtgat
     1621 tgtgctcggc tgccccgggc gcgtcccctg gcagacacca ttcggcgcga aggctgcaca
     1681 gtcgctcgat cagttccgtg tgttccgcat tgatcgcccg gcggtccgga tcgccgagtc
     1741 cctggatatt gtaggcgtgc ggtcccgggt ggatcaggaa cggatacggg ctgcccggct
     1801 gggtgccccc gacgtcgcga gcgctcacgt tcggcgcgta gtacatgatg tgcggatagt
     1861 tcacggtgcc gacctgatcg ttgtcctccg gcgcggtgta tgaccggaac acgggtgaca
     1921 acatatagga gatgcccgcg cgcgccgggg ccttgtagta gccggtcgcg tagcgctcct
     1981 ggatgagagc cttgacagcg gaggccggga ctccgtccgc ctgcatctcg gcggcgtcga
     2041 agaacaccgg caggatcgcg tcggctccgg cgctgtcgaa agcgaccgga tagatgatgt
     2101 cgtcgcgata gtcagtcggc atccaggcgc cgcggaacgc atcgtccccg tttctcgcga
     2161 cgagggcgtg gaaaccgttg gttccttcgc ggacccgttc gaagccctcg ttcggatcga
     2221 gcacatagac ggtggcctcg gcccgcagat gcggtggaag cgcgctgagc gccagttcga
     2281 tctcgag
//
LOCUS       DASVVN010000090         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_82249, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000090 DASVVN010000000
VERSION     DASVVN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82249"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VF686_01405"
     CDS             complement(<1..992)
                     /locus_tag="VF686_01405"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8660493.1"
                     /translation="MSIDVVIVSAARTPVGSFLGGLSSLPASKLGEVAIRAALERAGV
                     DAADVDEVILGHVLQAAAGQGPARQAAMGAGIPKETAAWSLNQICGSGLRAVAIGAQQ
                     IALGDAKIVVAGGQESMSQAPHAQHLRSGQKMGDLSLVDTMIRDGLWDAFNGYHMGQT
                     AENVAEKFHISRADQDDFAVASQHKAEAAQKAGRFDDEIAPVTIASKKGDVVVDKDEF
                     IRHGATLEAMEKLRPAFTKEGSVTAANASGLNDGAAALVLMSADEARARGLEVLGRIV
                     SSATAGVDPALMGTGPIPASKKALEKAGWTVADLDLVESNEAFAAQSVCVLRELG"
     gene            1210..1917
                     /locus_tag="VF686_01410"
     CDS             1210..1917
                     /locus_tag="VF686_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX8660494.1"
                     /translation="MLKRLKNVFVQVCVPDELDMAEHYRTPAEKTADLVVHVVGLTLA
                     AVGGIVLAVLAAMYVGMGAVAATATYALCLVAMLTVSTIYNQTKPCAARPILRRMDEA
                     AIFLMIAGSYTPFTTQRFEGGWAIGFTLLVWAIAIAGVGAKLVAPRISDAFWAGVYAL
                     FGWLAVIALKPMMDTVHPLALGLLVLGGLIYTAGVFVFISPKVKFRRAIWHGFVVVAA
                     AAHYTAVLVALGFAPVA"
     gene            complement(1926..2471)
                     /locus_tag="VF686_01415"
     CDS             complement(1926..2471)
                     /locus_tag="VF686_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8660495.1"
                     /translation="MKRLLLGAGLVWLGQRALAGHLPRFQLYSHSFEAGRTIGSPQVF
                     DSWGCTGGNRSPHLAWSEPPPETESLAVTCFDPDAPTGSGWWHWLAFNLPADLRSLPE
                     NASAEGLPGGAVQARNDYGLTGYGGPCPPEGDPPHRYIFTVWALDAAIELGLDANGAQ
                     VGFNVRQKAVGKAVTVGRYGR"
     gene            2555..>4418
                     /locus_tag="VF686_01420"
     CDS             2555..>4418
                     /locus_tag="VF686_01420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX8660496.1"
                     /translation="MADDNFIQITPFIYVENLEEGLAFFTDVLGFEAPQQHRYRDYAY
                     LQRESVGIRMLAGQGEDGAPRGNRRFAYYIDVRDVDRLHAELKPKLDRLDPGRVTGPV
                     NQPYRQRELMIVAPDNNLIVFGQAFARPRAPAPGFPGAALCSRARDDSMTKPIPPNPE
                     GERIHDELLSDALSRRYLAYALSTITNRALPDVRDGLKPVHRRLLYAMSNMRLTPAAA
                     TRKCAKVVGEVMGNFHPHGDSSIYEALVRLAQEFAQRYPLIEGQGNFGNIDGDNAAAM
                     RYTECRLTAAAQLLLEGIDEDAVDFRPTYDESDSEPVVLPGGFPNLLANGSSGIAVGM
                     ATSIPPHNAAELIDACLVLLARPEANTADLMERMLGPDFPTGGVCVESRASLLETYET
                     GRGGVRLRARWIKEELGRGVWRVVVTEMPYQVQKAKLIEDLAGLIDQKKAPLLADVRD
                     ESTEDVRLILEPRSRSVDPEAFMESLFRLSDLETRFPVNMNVLDARGTPRVMGLKPAL
                     LAFLDHRRDVLQRRSRWRLGHIERRLHILEGMLIAYLNLDEVIRIVRYEEHPKAELIR
                     AFVLSEEQAEAILNTRLRSLARLEEMQIKGEHDTLVAELEDITELLGSERRQWSK"
BASE COUNT          668 a         1581 c         1487 g          682 t
ORIGIN      
        1 ccgagttcgc gaaggacgca cacgctctgg gcggcgaagg cttcgttgga ttcgaccaga
       61 tcgagatcgg cgacggtcca gccggccttt tccagcgcct tcttcgaggc cgggatcggg
      121 ccggtgccca tgagcgccgg atcgacgccc gcggtggccg atgagacgat ccggccgagg
      181 acctcaaggc cccgcgccct ggcctcgtca gcgctcatga ggacgagggc cgccgcgccg
      241 tcgttcaggc ccgaggcgtt ggcggcggtg accgagcctt ccttggtgaa ggccgggcgc
      301 agcttctcca tggcttcgag ggtcgcgccg tggcggatga actcgtcctt gtcgacgacg
      361 acgtcgccct tcttcgaggc gatggtgacc ggcgcgatct cgtcgtcgaa ccggcccgcc
      421 ttctgggccg cctcggcctt gtgctggctg gcgacggcga agtcgtcctg atcggcgcgg
      481 ctgatgtgga acttctcggc cacgttctcg gcggtctggc ccatgtgata gccgttgaag
      541 gcgtcccaca ggccgtcccg gatcatggtg tcgaccagcg acagatcgcc catcttctgg
      601 ccggaccgca gatgctgggc gtggggcgcc tggctcatgc tctcctgtcc gccggcgacg
      661 acgatcttcg cgtcgccgag ggcgatctgc tgggccccga tggccacggc ccggaggccc
      721 gagccgcaga tctggttcag gctccaggcc gccgtctcct tcggaatgcc cgcgcccatg
      781 gccgcctgcc gcgccggccc ctgacccgcc gccgcctgaa gcacgtgacc aagaatgacc
      841 tcgtcgacgt cggcggcgtc gacgcccgcc cgctccagcg ccgcccggat ggcgacctcg
      901 ccgagcttcg acgccggcag gctggacaga ccgccgagga aggagccgac cggcgtgcgc
      961 gcggcggaga cgatgacgac gtcgatagac atgaagaacc tctggagcgg gcccgggcgg
     1021 gggagcgccg gaccatggaa acgaaacctc tacgacccat gtggtcgtta ggtcccgcga
     1081 aacaaatcgt ggcgtttctg ccgcatcgcg aagccttcgt cacgcctgac ggcgctgttc
     1141 accgcgtggt cactggcccg gccccatctt cacgggaaca aaccgggcca gaaaggcttg
     1201 atggttgcca tgctgaagcg actgaaaaac gtgttcgtcc aggtctgcgt gccggacgag
     1261 ctggacatgg ccgagcacta tcgcacgccc gccgagaaga ccgccgatct agtcgtgcat
     1321 gtcgtcggcc tgacgctcgc cgccgtcggc gggatcgtgc tggcggtgct ggcggcgatg
     1381 tatgtcggga tgggcgccgt ggcggccacc gcgacctacg ccctgtgcct ggtcgccatg
     1441 ctgaccgtct cgactatcta caatcagacg aagccctgcg ccgcgcggcc gatcctgcgc
     1501 cgcatggacg aggcggcgat cttcctcatg atcgccggca gctacacccc tttcacgacc
     1561 cagcggttcg aaggcggctg ggccatcggc ttcaccctgc tggtctgggc aatcgccatc
     1621 gcgggcgtcg gcgccaagct cgtggcgccg cgtatttccg acgccttctg ggccggcgtc
     1681 tatgcgctgt tcggctggct ggccgtgatc gcgctgaagc cgatgatgga caccgtccac
     1741 ccgctcgccc tgggcctgct ggtgctgggc gggctgatct acaccgccgg ggtgtttgtc
     1801 ttcatcagcc cgaaggtcaa gttccgccgc gccatctggc acggcttcgt cgtcgtcgcc
     1861 gccgccgccc actacaccgc cgtgctggtc gccctcggct tcgcccccgt cgcctagccg
     1921 gttgcctagc ggccgtagcg ccccaccgtc accgccttgc cgaccgcctt ctggcggacg
     1981 ttgaacccga cctgcgcgcc gttggcgtcc aggccgagct cgatggccgc gtcgagcgcc
     2041 cagaccgtga agatgtagcg gtgcggcggg tcgccctcgg gcgggcaggg gccgccgtag
     2101 ccggtgaggc catagtcgtt gcgcgcctgc accgcgccgc cgggcagtcc ttcggcgctg
     2161 gcgttctcgg gcagtgagcg caggtcggcc ggcaggttga aggccagcca gtgccaccag
     2221 cccgatcccg tcggcgcgtc cgggtcgaag caggtgacgg ccaggctctc cgtctccggc
     2281 ggcggctcgc tccaggccag gtgcggcgag cggttgcccc cggtgcagcc ccagctgtcg
     2341 aacacctgcg gggaaccgat ggtccgcccc gcctcgaagc tgtggctgta gagctggaac
     2401 cggggcaggt gacctgccag cgcccgctgc cccagccaga ccagcccagc gcccagaaga
     2461 agccgcttca tcgtcacctc gcgccgtccg ccccgtcctt tagcgaaacc aggtgcgccc
     2521 cggctataag gcgcgcctga aacgggaggg cccgatggcg gacgacaact tcattcagat
     2581 cacgccgttc atctacgtcg agaacctcga ggaaggcctg gccttcttca ccgacgtgct
     2641 gggcttcgaa gcgccgcagc agcaccgcta ccgcgactac gcctacctgc agcgggagag
     2701 cgtgggcatc cggatgctgg cgggccaggg cgaagacggg gctccgcggg gcaatcgccg
     2761 cttcgcctac tacatcgacg tccgcgacgt ggaccggctc cacgccgagc tgaagccgaa
     2821 gctggaccgg ttggaccccg gccgggtgac cggcccggtg aaccagccct accgccagcg
     2881 cgagctgatg atcgtcgccc ctgacaacaa cctcatcgtg ttcggccagg cgttcgcccg
     2941 ccccagagcc cccgcccccg gttttcccgg cgctgcgcta tgctcgcgcg ccagagatga
     3001 ttccatgacc aagcccatcc cacccaatcc cgaaggcgag cggatccacg acgagctgct
     3061 ctccgacgcg ctctcccggc gctacctggc gtacgcgttg tcgaccatca ccaaccgggc
     3121 gctgccggac gtgcgcgacg ggctgaagcc ggtgcaccgg cggctgctct acgccatgag
     3181 caacatgcgg ctcaccccgg ccgcggccac ccgcaagtgc gccaaggtgg tcggcgaggt
     3241 gatgggcaac ttccacccgc acggcgacag ctcgatctac gaggcgctgg tgcgcctggc
     3301 ccaggagttc gcccagcgct acccgctgat cgaggggcag ggcaacttcg gcaacattga
     3361 cggcgataac gccgcggcca tgcgctacac cgagtgccgg ctgaccgccg ctgcccagct
     3421 gctgctcgag ggcatcgacg aggacgcggt cgacttccgt cccacctacg acgagagcga
     3481 cagcgagccg gtggtgctgc ccggcggctt ccccaacctg ctggccaacg gctcgtcggg
     3541 gatcgcggtg ggcatggcca cctccatccc gccgcacaac gccgctgagc tgatcgacgc
     3601 ctgcctggtg ctgctggcgc ggccggaggc caacaccgct gacctgatgg agcgcatgct
     3661 cggcccggac ttcccgaccg gcggcgtctg cgtcgaaagc cgcgcctcgc tgctcgagac
     3721 ctacgaaacc gggcgcggcg gcgtgcggct gcgcgcgcgc tggataaagg aagagctcgg
     3781 ccgcggcgtc tggcgcgtcg tcgtcaccga gatgccctac caggtgcaga aggcgaagct
     3841 gatcgaggac ctcgccggtc tcatcgacca gaagaaggcg ccgctgctcg ccgacgtgcg
     3901 ggacgagtcg accgaggacg tgcgcctgat cctcgagccg cgctcgcgca gcgtcgatcc
     3961 ggaagccttc atggagagcc tgttccgcct ctccgacctg gagacccgct tccctgtcaa
     4021 catgaacgtg ctcgacgcgc gcggcacgcc gcgggtgatg gggctgaagc cggcgctgct
     4081 ggccttcctc gaccaccggc gcgacgtgct gcagcggcgc tcgcgctggc ggctcggcca
     4141 catcgagcgg cggctgcaca tcctcgaagg gatgctgatc gcctacctga acctcgacga
     4201 ggtgatccgc atcgtccgct acgaggagca tccgaaggcg gagctgatcc gcgccttcgt
     4261 gctctccgag gagcaggccg aggcgatcct caacacccgc ctgcgcagcc tcgccaggct
     4321 cgaggagatg cagatcaagg gcgagcacga cacgctcgtg gccgaactgg aggacatcac
     4381 cgagttgctc ggctccgagc gccgccagtg gagcaagg
//
LOCUS       DASVVN010000091         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_3315, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000091 DASVVN010000000
VERSION     DASVVN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3315"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF686_01425"
     CDS             <1..290
                     /locus_tag="VF686_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660497.1"
                     /translation="YLEMARRSRRFGFAGASCIHPSQIPLLNEAFSPTDKEVAYATRV
                     LAEFDAAVASGRGAVALDGKMIDAPIVARARDLLDRHAAIRSRGSRPAPNV"
     gene            337..>1529
                     /locus_tag="VF686_01430"
     CDS             337..>1529
                     /locus_tag="VF686_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660498.1"
                     /translation="MTATTSPLPARATKARYAMLALIAFATMLNYLDRTILGIAGAHG
                     LQTDLRIDPALWGIVLSAFGWTYAAAQLPGGIFLDRLGTRVTYFWAVTLWSIFTALQG
                     LAFNTTSLVLARLGMGAAEAPCFPANSRVLVSWFPQRERARANSVYGVGMYVGNGLLG
                     PLVYWFTAAYGWRALFIAAGAIGVGFALIWWFLYREPEESKWINDAEREEIRAGGGIS
                     MAGEKTPFRWSDVGWLCKQRQVVGASIGQFATNSTLVFFLTWFPTYLVNERGMAFIKA
                     GFMASLPFIAAAVGVLFGGQLSDYLVKKYGLTVGRKTPVIVGLALSALIATAAFVESN
                     TIVIAVMSIAFFGQGMSNLGWTLVSELAPRKLIGTTGGVFNFVTNLAGIVTPIIIGFV
                     VAATGS"
BASE COUNT          213 a          569 c          481 g          266 t
ORIGIN      
        1 cttatctgga gatggcgcgg cgctcgcggc gattcggctt cgccggcgcc agctgcatcc
       61 accctagtca gatccctctc ctcaacgagg ccttctcgcc gaccgataag gaggtcgcct
      121 atgcgacccg cgtgctggcc gagttcgacg cggcggtcgc ctccggccgc ggcgccgtcg
      181 cgctggacgg caagatgatc gacgccccga tcgtcgcccg tgcgcgcgac ctactcgatc
      241 gccacgccgc catccggtca cgcggaagcc gccccgcccc caacgtctag acaatccgcg
      301 ccaagcggtg accgccagaa ggtccgagga aacgccatga ccgccacgac cagtcctctg
      361 ccggcccggg ccaccaaggc gcgctacgcc atgctggcgc tgatcgcctt cgccaccatg
      421 ctgaactatc tggataggac gatcctcggc atcgccgggg cgcacggcct gcagacggac
      481 ctgcgcatcg acccggccct gtggggcatc gtcctgtcgg cgttcggctg gacctatgcg
      541 gcggcgcagc tgccgggggg catcttcctc gaccggctgg gcacccgcgt cacctacttc
      601 tgggcggtga ccctgtggtc gatcttcacc gccctgcagg ggctggcctt caacaccacc
      661 tcgctggtgc tggcgcgcct gggcatgggc gcggccgaag ccccctgctt tcccgccaac
      721 agccgggtgc tggtcagctg gttcccgcag cgcgagcggg ccagggccaa cagcgtctat
      781 ggcgtgggca tgtacgtggg caacggcctg ctgggcccct tggtctattg gttcaccgcc
      841 gcctacggct ggcgcgccct gttcatcgcc gccggggcca tcggcgtcgg cttcgccctg
      901 atctggtggt tcctctaccg cgagcccgag gagtccaagt ggatcaacga cgccgagcgc
      961 gaggagatcc gcgccggcgg cggcatcagc atggccggcg agaagacccc cttccgctgg
     1021 tccgacgtgg gctggctgtg caagcagcgc caggtggtgg gcgcctccat cggccagttc
     1081 gccaccaact ccaccctggt cttcttcctg acctggttcc cgacctacct ggtcaacgag
     1141 cgcggcatgg ccttcatcaa ggctggcttc atggcctccc tgcccttcat cgccgccgcc
     1201 gtcggcgtgc tgttcggcgg ccagctgtcg gactacctcg tcaagaagta cggcctgacc
     1261 gtcgggcgca agacgccggt gatcgtcggg ctggcactgt cggcgctcat cgccaccgcc
     1321 gccttcgtgg agtccaacac catcgtcatt gcggtgatgt cgatcgcgtt cttcggccag
     1381 ggcatgtcca acctcggctg gaccctcgtc tccgagctcg cgccgcgaaa gctcatcggc
     1441 accaccggcg gggtgttcaa cttcgtcacc aacctggccg gcatcgtcac cccgatcatc
     1501 atcggcttcg tcgtcgccgc aaccggctc
//
LOCUS       DASVVN010000092         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_398307,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000092 DASVVN010000000
VERSION     DASVVN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398307"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VF686_01435"
     CDS             complement(<1..781)
                     /locus_tag="VF686_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8660499.1"
                     /translation="MTDLSSTRPAGLPQPRSYNGVNWIGVRTLYLREVRRFWKVGAQT
                     VAGPVVTTLLYMLVFVVALRGTRPPLHGTPFAEFVAPGLIMMAMLNNAFANASSSLIQ
                     AKVMNTATDFLTPPLSPLELTIGFSLGAATRGLVVGVVTAICVMPFARLEVANLGLIL
                     WFGLLACLIMGMLGVFAGLWAQKFDQLAAVQNFVIMPMTFLSGTFYLVERLPEPFATI
                     SHFNPIFYLIDGFRYGFIGHAEGSLLIGVIMSAALTVVMAIC"
     gene            999..1505
                     /locus_tag="VF686_01440"
     CDS             999..1505
                     /locus_tag="VF686_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX8660500.1"
                     /translation="MTAGWTEDRVGALKKLWLEGQSASQIAKQLGGGVTRNAVIGKVH
                     RLGLSGRAAPSQPARATFRPSRPRPAQPTQAPSAPRRIEAVQPRAVAAPSVPAPMPDL
                     PGTATVMTLGAHMCKWPIGDPSSNEFSFCGRRSSEGVYCVEHARVAYQPQVKRGATDL
                     ARSLRRYI"
BASE COUNT          303 a          566 c          541 g          252 t
ORIGIN      
        1 agcagatggc catgacgacc gtgagggcgg ccgacatgat cacgccgatc agcaggcttc
       61 cttccgcatg gccgatgaag ccgtagcgga agccgtcgat cagatagaag atcgggttga
      121 agtggctgat cgtggcgaac ggctcgggca gccgctcgac cagatagaag gtgccgctca
      181 ggaaggtcat cggcatgatg acgaaattct gcaccgcggc cagttgatcg aacttctgcg
      241 cccacaggcc ggcgaagacg cccagcatcc ccatgatcag gcaggccagc aggccgaacc
      301 aaaggatcag gccgaggttc gccacctcca gccgcgcgaa cggcatgacg cagatcgcgg
      361 tcacgacgcc gaccaccaga ccgcgcgtcg cggcgccgag tgaaaagccg atggtcagct
      421 ccagcgggct gagcggcggc gtcaggaagt cggtcgccgt attcatcacc ttggcctgga
      481 tcaggctgga cgaggcgttg gcgaaggcgt tgttcagcat ggccatcatg atcaggcccg
      541 gcgccacaaa ttcggcgaag ggcgtgccgt gcaacggcgg ccgcgtgccg cgcagggcga
      601 ccacgaagac cagcatgtac agcagggtgg tcaccaccgg cccggccacc gtctgggccc
      661 cgaccttcca gaagcgccgc acctcccgca ggtacagggt cctgaccccg atccagttga
      721 caccgttgta gctgcggggc tgcggcaggc cggcggggcg ggttgaactc aggtcggtca
      781 tggcccgcga aatgcgctcg cccgctccgc ttcgcaagag ccgccgaaag cgagccgaac
      841 cgtagagctg ttaaccatct ctacatctgg ttcctgtgga cggactgatt cgcacatctt
      901 gcgtctgtta gggggtcgtt tacgattagt ggtaggtcga gaccccggga gggacatctg
      961 gaccccatat attgaatccg agacccggag ggttggctat gaccgcaggc tggactgaag
     1021 accgcgtcgg cgcgctgaag aaactctggc tcgagggcca gtcggccagc cagatcgcca
     1081 aacagctggg tggcggcgtc acccgcaatg ccgtcatcgg caaggtgcac cgtctgggcc
     1141 tgtcgggccg ggccgcgcct tcgcagccgg cgcgcgccac cttccgcccg tcgcgtccgc
     1201 gccccgcaca gccgacccag gctccgtcgg ccccgcgccg catcgaggcg gtccagccgc
     1261 gcgccgtggc ggccccgtcg gtcccggccc ccatgccgga cctgcctggc accgccacgg
     1321 tgatgaccct cggcgcccat atgtgcaaat ggccgatcgg cgacccgtcc tcgaacgaat
     1381 tcagcttctg cggccgccgc tcctcggaag gcgtctactg cgtcgaacac gcccgcgtcg
     1441 cctaccagcc gcaggtcaag cgcggcgcga cggatctggc ccgcagcctg cggcgctaca
     1501 tctagggggc gctatagctc gcgacgcggt cgctcactgc ttgagcgccg tcgatcagga
     1561 ttcatttctt gcgctcaagc aggcgacagc cgttagcgcc caaagaaatg tcatgaacgg
     1621 ctcctagaga gccgccctcc gcctcatggc cgcacccgca cg
//
LOCUS       DASVVN010000093         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_213970,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000093 DASVVN010000000
VERSION     DASVVN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213970"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..606)
                     /locus_tag="VF686_01445"
     tRNA            complement(531..606)
                     /locus_tag="VF686_01445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(571..573),aa:Phe,seq:gaa)
     gene            complement(670..834)
                     /gene="yacG"
                     /locus_tag="VF686_01450"
     CDS             complement(670..834)
                     /gene="yacG"
                     /locus_tag="VF686_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX8660501.1"
                     /translation="MATCPICRRHPADEKYKPFCSKRCADVDLQRWFTGGYVLPDSGR
                     DVPTGDPEED"
     gene            complement(837..>1532)
                     /locus_tag="VF686_01455"
     CDS             complement(837..>1532)
                     /locus_tag="VF686_01455"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8660502.1"
                     /translation="PRLLAPAPTLMATLARMAPGVAPIAGKAAIEAGWAAVEESQAHP
                     TIPGGTVAVERTRAMVTVDIDLNSHDMGRGRERDAANRRGLLEAARAIRLRRWGGLVA
                     IDLIGAGHDGAAILKAAKAAFGDDPRIAFGPVSRFGVLQVALRWEATPLEEALYQHPQ
                     IHTPWPHQAAQEAARRIRFELLSDTTRARITVRCPDEVARLAAPLVAQLGPRAHLKVD
                     DSLRTGEHVIEAD"
BASE COUNT          242 a          517 c          516 g          257 t
ORIGIN      
        1 ggagcggcgg cggcgtcagc ggccggagca gctgcagcgg cggccgggtc ggcggcgggg
       61 gcatcctgcg cgagaaccgg cgtaccagcc atgaggacgg cggcgccggc catggccagg
      121 aggatgttgg ttttcttgat atcgaacata gttcgccagt tcctgctgtt ttggtctgac
      181 ttgtggaacc caaaagaaac ggccgcagac gacgaccgcc ctcgatgaaa ttctttcgcc
      241 cgtaggacag ggttaaggaa ccccggtctg cggaacgcct gctgacgctt ccggttgcca
      301 tgcggcttcc ggtgcggcgc cgatgaacag ggaaaccgtt ttccctctcc gccaactgcc
      361 cgccaaggaa agcggtcaag ctcctttggc gaacgcatac cagagcgtca agtgcgggga
      421 cgtgcttttc ccctcgcggg ggagccacgg ctccaatcgc tgaagattgg ggccgaaagc
      481 gacattttca tgatggcgga cgacccgtcc cagcattcgg gaacgggcga tggtgcctgg
      541 gggcggattc gaaccaccga cacgtggatt ttcaatccac tgctctacca actgagctac
      601 ccaggcgcac acgtcgcggc gcgaggcggc gtctataggc gagcgctccg ggggtgtcca
      661 gccgtcagat cagtcttctt cagggtcgcc cgtcggaacg tccctgccac tgtcgggcag
      721 gacgtatccg cccgtgaacc agcgctgcag atcgacgtcg gcgcagcgct tggagcagaa
      781 gggtttgtat ttttcgtccg ccgggtggcg gcggcagatg ggacaggtcg ccatggtcag
      841 tccgcctcga tcacgtgttc gccggtgcgc aggctgtcgt cgaccttcag atgggcgcgc
      901 ggacccagct gcgccaccag cggcgcggcc agccttgcca cctcatccgg gcatcgcacg
      961 gtgatccgcg cgcgggtcgt gtcagataat agttcgaatc tgatccgccg cgctgcttcc
     1021 tgcgcagcct gatgcggcca cggggtgtga atctgcgggt gctggtaaag cgcctcttcc
     1081 agtggcgtcg cctcccaccg gagggccact tgcagcacgc cgaagcggct gaccgggccg
     1141 aaggcgatcc gcgggtcgtc gccgaacgcc gccttcgccg ccttcaggat cgccgcgccg
     1201 tcgtgccccg cgccgatcag gtcgatcgcg accagtccgc cccagcgcct caaccggatc
     1261 gcccgcgctg cctccagcaa gccccggcga ttcgcggcgt cccgctcgcg cccccggccc
     1321 atgtcatggc tgttcaggtc gatatcgacc gtgaccattg cccgggtccg ctcgaccgcc
     1381 accgtgccgc caggaatagt cggatgagcc tgggactcct cgacggcggc ccagcccgcc
     1441 tcgatggccg ccttgccggc gatcggcgca acgccggggg ccatccgggc cagcgtcgcc
     1501 ataagggtgg gcgccggcgc caggaggcgc gg
//
LOCUS       DASVVN010000094         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_266398,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000094 DASVVN010000000
VERSION     DASVVN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266398"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1272
                     /gene="trpB"
                     /locus_tag="VF686_01460"
     CDS             88..1272
                     /gene="trpB"
                     /locus_tag="VF686_01460"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8660503.1"
                     /translation="MPDAEGRFGPYGGRFVAETLMPLVHELNAAYEAAKADPSFQAEL
                     DGFLTDYVGRPSPLYLAERLTEHHGAANIWFKRDELNHTGAHKINNCMGQILLAMRMG
                     KTRIIAETGAGQHGVATATVCARFGLQCVVYMGATDVERQSPNVFRMKLLGAEVVPVT
                     AGRATLKDAMNEAMRDWVTNVEDTYYLIGTAAGPHPYPAMVRDFQSVIGRETRVSMLA
                     WREKLPDAVVAAIGGGSNAIGLFHPFIEDAGVRLIGVEAAGRGLDGPDHAASLQGGRP
                     GVLHGNRTYLLQDGDGQILEGHSISAGLDYPGIGPEHAWLKDIGRAEYRAATDAEALE
                     AFQLCSKLEGIIPALEPSHALARVGEIAKEVGKGGDVVLNMCGRGDKDIFAVAKHLGV
                     EL"
     gene            1274..1738
                     /locus_tag="VF686_01465"
     CDS             1274..1738
                     /locus_tag="VF686_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660504.1"
                     /translation="MKIAGLAAAAALMAAPVWAQAPAAPAPAAPAVVSPDAMVLPGAT
                     LAPDCGGLYNLAGRAFCVSAPLAGIGALADTYVADLQTKGWLTAGGDDNRVVFVRRRD
                     GGGCDGLQMQAFYDTSRPAGPAAIGYLGFGPIPGDVCAAQPAAPAAAVPPAQ"
     gene            1735..>2333
                     /gene="trpA"
                     /locus_tag="VF686_01470"
     CDS             1735..>2333
                     /gene="trpA"
                     /locus_tag="VF686_01470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8660505.1"
                     /translation="MTTARIDARFAALKAEGRAGFVAYVMAGDPTRDQALDILRGLPG
                     AGADIIELGFPFSDPMAEGPPIQRAALRGLKAGLTLKGTLALAADFRAGDADTPVILM
                     GYLNPIETYGYDAFARDAAASGMDGLIVVDCPPEEAGPLSDALDAASVSLIRLATPTS
                     DDARLKIIAERTSGFVYYVSVAGVTGVKEADAAVVAPQV"
BASE COUNT          343 a          786 c          861 g          343 t
ORIGIN      
        1 ctcgatactg tccggaccgc ctgatttccg ccgctccccc gcgccaaggc taccgacgtg
       61 aacgccatca tccccaacca gtacgacatg cccgacgccg agggccgttt cggtccctat
      121 ggcggccggt tcgtggccga gaccctgatg ccgctggttc acgagctgaa cgcggcctat
      181 gaggcggcca aggcggatcc gagctttcag gccgagctgg acggattcct gaccgactat
      241 gtcggccggc cgagccccct gtatctggcc gagcggctga cggagcacca cggggcggcg
      301 aacatctggt tcaagcgcga cgagctgaat cacacgggcg cgcacaagat caacaactgc
      361 atgggccaga tcctgctggc catgcgaatg ggcaagacgc ggatcatcgc ggagaccggg
      421 gccggccagc acggcgtggc gacggcgacc gtctgcgccc gcttcggact gcaatgcgtc
      481 gtctacatgg gcgcgaccga cgtcgagcgg cagtcgccga acgtcttccg catgaagctt
      541 ctgggggcag aggtcgtgcc ggtgacggcg ggccgggcga cgctgaagga cgccatgaac
      601 gaggcgatgc gcgactgggt caccaatgtc gaggacacct attatctgat cggcacggcg
      661 gcggggccgc atccctatcc ggccatggtg cgcgacttcc agtcggtgat cgggcgggag
      721 acgcgggtgt cgatgctggc ctggcgcgag aagctgccgg acgcggtggt ggcggcgatc
      781 ggcggcggct cgaacgccat cggcctgttc caccccttca tcgaggacgc cggcgtgcgg
      841 ctgatcgggg tcgaggcggc cgggcgcggg ctggatggcc cggaccacgc tgccagcctg
      901 cagggcgggc ggccgggggt gctgcacggc aaccgcacct atctgctgca ggacggcgac
      961 ggccagattc tggaaggcca ttcgatctcg gccggtctcg actatccggg catcggcccc
     1021 gaacacgcct ggctgaagga tatcggccgc gccgaatacc gcgccgccac cgatgcggag
     1081 gcgctggaag ccttccagct gtgctcgaag ctggagggga tcattcccgc gctggagccc
     1141 agccacgcgc tcgcccgggt gggcgagatc gcgaaggaag tgggcaaggg cggcgatgtc
     1201 gtgctgaata tgtgcgggcg gggcgacaag gacattttcg ccgtggcgaa acatctgggc
     1261 gtggagttgt agaatgaaga tcgcaggact ggccgcggcg gcggctctca tggccgcgcc
     1321 cgtctgggcg caggcgccgg cggctcccgc ccccgccgcc cccgccgtgg tatcgccgga
     1381 cgccatggtg cttccgggcg cgacgctcgc gcccgattgc ggcggtctgt acaatctggc
     1441 cggccgcgcc ttctgcgtca gcgcgccgct ggcggggatc ggcgccctgg ccgacaccta
     1501 tgtcgccgac ctgcagacca agggctggct gacggccggc ggcgacgaca accgtgtggt
     1561 gttcgtgcgc cggagggacg gcggcggctg cgacgggctg cagatgcagg ccttctacga
     1621 cacaagccga cccgcgggtc cggcggcgat cggctatctc ggcttcggcc cgatcccggg
     1681 cgacgtctgc gccgcccagc cggccgcccc ggcggcggcg gttcctccgg cgcaatgacc
     1741 acggcgcgta tcgacgcccg gttcgcggcg ctgaaggccg agggccgggc cggcttcgtg
     1801 gcctatgtca tggccggaga cccgacgcgg gatcaggcgc tggacatcct gcgcggcctg
     1861 ccgggcgcgg gggccgacat catcgagctg ggctttccct tctcggatcc gatggccgag
     1921 gggccgccga tccagcgcgc ggcgctgcgc gggctgaagg ccgggctgac gctgaagggg
     1981 acactggccc tggccgccga tttccgcgcc ggcgacgccg acacgccggt gatcctgatg
     2041 ggctatctca acccgatcga gacctatggc tatgacgcct tcgcccgcga cgcggcggcc
     2101 agcggcatgg acgggctgat cgtcgtcgac tgtccgccgg aagaggccgg gccgctgtcg
     2161 gacgcgctgg acgcggcgtc ggtgtcgctg atccggctgg ccacgccgac ctcggacgac
     2221 gcgcggctga agatcatcgc cgaacggacc tcgggcttcg tctactatgt ctcggtcgcc
     2281 ggggtgaccg gggtcaagga ggccgacgcc gccgtggttg ccccccaggt cga
//
LOCUS       DASVVN010000095         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_371846,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000095 DASVVN010000000
VERSION     DASVVN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371846"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VF686_01475"
     CDS             <1..823
                     /locus_tag="VF686_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8660506.1"
                     /translation="DALMFTGSGAAGAHFRRKFADDPHVILALELGGNNPRVVWDAAD
                     AEAVAGIVVQSAFITTGQRCSCARRLIVPAGPKGDAIIEAVIALTHRLIVGRWNSDPE
                     PYMGSLISARAADAVLGQAADRCNAGARVLMTFGHLDTESEAFVLPGIIDTTGVDVPD
                     EEIFGPILQVRRVGSLDEAIREANNTRYGLSAGLVSDDPANWDRFIRRIRAGVVNFNR
                     PTTGAAGDMPFGGLGASGNHRPSAYYAADYCAYPVASFEAGSIKNIESEIKGLLP"
     gene            820..2157
                     /gene="astB"
                     /locus_tag="VF686_01480"
     CDS             820..2157
                     /gene="astB"
                     /locus_tag="VF686_01480"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX8660507.1"
                     /translation="MTSAIEANADGLIGPTHSYAGLSPGNLASSLNAGDASNPRAAVL
                     QGLDKMKRLADLGLPQFVLPPHERPNTPFLRSLGFTGSDAAVLEAAWKTAPSFAAAAC
                     SASPMWAANAATVTPSADAADGRVHFTPANLVTNLHRSLEHAQTKRALDALFPDPTRF
                     AVHDALPSVPHLADEGAANHVRLCADHGEPGVNVLVWGREAYEPWSGAFPARQTREAS
                     EAIARRHDAARAVFPRQSRAAIVGGTFHNDVVCVGARETLFFHKLAFEDTDATKAAIR
                     SAAKGLFEPIFIEVSAADLPLADAISSYLFNSMLVRIPGEDRLTLICPTETRDNLRSH
                     AVAERLAASNGPIGRVEYVDVRQSMRNGGGPACLRLRVVLTEVELAATNPAMRMTDAL
                     HARLSAWAGARYRDTLTPRDLADRALLDESRAALDELTGILGLGGGFYPFQRV"
     gene            2162..2728
                     /locus_tag="VF686_01485"
     CDS             2162..2728
                     /locus_tag="VF686_01485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660508.1"
                     /translation="MSAFSIRTATEADIPALVALMDRSISGPLAAFLTPDQITASRKL
                     MGLDSQIIADRTYFIVEVDGVLAGCGGWSRRITTYGGDHTPGREPALLHPGIDAARVR
                     AMYTAPEFVRRGIGRLILQVCEDAARAEGYDRVELVATMGGEPLYLASGYREIERFED
                     DRGGAAVPLVRMGKRLDEAGFGHPREPG"
     gene            2757..>2975
                     /locus_tag="VF686_01490"
     CDS             2757..>2975
                     /locus_tag="VF686_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660509.1"
                     /translation="MKPTPNPALVIGGVLSVLASLLHIACIFAGPDWYRFFGAGEEMA
                     TMAARGSIIPTLLTLGIATVLAIWAAYAF"
BASE COUNT          435 a         1157 c          928 g          455 t
ORIGIN      
        1 cgacgccctg atgttcaccg gctcgggcgc ggccggcgcc catttccggc gcaaattcgc
       61 cgacgacccg cacgtgatcc tggcgctgga gctcgggggc aacaatccgc gggtggtgtg
      121 ggacgccgcc gacgccgagg ccgtcgccgg gatcgtcgtc cagtccgcct tcatcaccac
      181 cggccagcgc tgctcctgcg cccgccgcct gatcgtgccg gcagggccga agggcgacgc
      241 catcatcgaa gccgtgatcg ccctcaccca ccggctgatt gtcggccgct ggaacagcga
      301 tccggagccc tatatgggct cgctgatctc cgcccgcgcg gccgatgccg tgctcggcca
      361 ggccgccgac cgctgcaacg ccggggcccg ggtgctcatg accttcggtc atctcgacac
      421 cgagagcgaa gccttcgtcc tcccggggat catcgacacc acgggcgtgg acgtcccgga
      481 cgaggaaatc ttcggcccca tcctgcaggt ccggcgcgtc ggttccctcg acgaggcgat
      541 ccgggaagcc aacaacaccc gttacggtct gtccgctggc ctggtcagcg acgatccggc
      601 caactgggac cgctttatcc gccgcatccg cgcaggcgtg gtcaacttca accgtccgac
      661 caccggcgcc gccggcgaca tgcccttcgg cggcctcggc gccagtggca accaccggcc
      721 gtcggcctac tacgccgccg actactgcgc ctatccggtg gccagtttcg aggccggttc
      781 gatcaagaac atcgaaagcg agatcaaggg cttgctgccg tgacctcggc tatcgaggca
      841 aacgccgacg gtctgatcgg accgacccat tcctacgccg gcctgtcgcc gggcaacctc
      901 gcctccagcc tcaacgccgg cgacgcgtcc aacccgcgcg cggccgtcct gcagggcctc
      961 gacaagatga agcggctggc cgacctcggc ctgccacagt tcgtcctgcc gccgcacgag
     1021 cggccgaaca cgccgttcct tcgcagcctc ggtttcaccg gctctgacgc cgccgtgctg
     1081 gaagccgcct ggaagaccgc cccgtctttc gccgccgcgg cctgctccgc ctcgccgatg
     1141 tgggccgcca acgccgcgac ggtgacgccg tccgccgacg ctgccgacgg ccgcgttcat
     1201 ttcacgccgg ccaacctcgt caccaacctg catcgctcgc tggaacatgc gcagacgaag
     1261 cgtgcgctgg acgccctctt cccggacccg acgcggttcg ccgtccacga cgccctgccg
     1321 tcggtccccc acctcgccga cgagggcgcc gccaaccacg tccgcctgtg cgccgaccac
     1381 ggcgaacccg gcgttaacgt cctggtctgg ggccgcgagg cctatgagcc atggtccggc
     1441 gccttcccgg cccgccagac gcgcgaggcc tccgaggcca tcgcccgccg tcacgacgcc
     1501 gcacgtgcgg tcttcccgcg ccagtcccgc gccgccatcg tcggcgggac cttccacaac
     1561 gacgtcgtct gcgtcggcgc gcgggagacc ctgttcttcc acaaactcgc cttcgaggac
     1621 acggacgcga caaaggccgc catccgcagc gccgcgaagg gcctgttcga gccgattttc
     1681 atcgaggttt ccgccgccga cctgccgctg gcggacgcca tctccagcta tctgttcaac
     1741 tccatgctgg tgcggattcc gggcgaggac cggctgaccc tgatctgccc caccgagacc
     1801 cgcgacaacc tgcgcagcca cgccgtcgcc gaacggctcg ccgcctccaa cggccccatc
     1861 ggccgggtgg aatacgtcga tgtgcgccag tcgatgcgca atggcggggg acccgcctgc
     1921 ctgcgcctgc gcgttgtcct gaccgaggtc gaactggccg cgacgaaccc ggctatgcgg
     1981 atgacagacg cccttcacgc ccgcctctcc gcctgggccg gggcccggta tcgcgacacg
     2041 ctgacgcccc gggacctcgc cgatcgcgcc ctgctggacg aaagccgggc cgcgctggac
     2101 gaactgaccg gcatcctcgg tctgggtggc ggcttctacc ccttccagcg ggtctgaccc
     2161 catgtccgcc ttctctatcc gcaccgcgac cgaggccgac atccccgccc tcgtcgccct
     2221 gatggaccga tccatctccg gccccctcgc cgccttcctg acgccggacc agatcaccgc
     2281 cagccgcaag ctgatgggtc tggacagtca gatcatcgcc gaccgcacct atttcatcgt
     2341 cgaggtcgac ggcgttctag cgggctgcgg cggctggagc cggcgcatca ccacctatgg
     2401 cggcgaccac actccgggcc gcgagcccgc cctgctgcat cccgggatcg acgccgcccg
     2461 cgtccgcgcc atgtacacgg cgcccgaatt cgtccgccgg ggcataggcc gcctgatcct
     2521 gcaggtctgc gaggacgccg cgcgcgccga gggctacgac cgcgtcgaac tggtcgccac
     2581 catgggcggc gagcccctct atctcgccag cggctatcgc gagatcgaac gcttcgagga
     2641 cgaccgcggc ggcgcggcgg tgcctctggt ccggatgggc aaacggctgg acgaggcggg
     2701 gtttggccat ccgcgcgaac ccggctagcg ttcgtacgac cgacggggga agcaacatga
     2761 aaccgacgcc caatccggcg cttgtcatcg ggggcgtgct cagcgtgctg gcgtccttgc
     2821 tgcacatcgc ctgcatcttc gcaggacctg actggtaccg cttcttcggc gccggcgaag
     2881 agatggcgac catggccgcg cgaggttcga ttatcccgac cctgctcacc cttggcatcg
     2941 cgaccgtgct ggcgatctgg gccgcctacg ccttc
//
LOCUS       DASVVN010000096         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_424120,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000096 DASVVN010000000
VERSION     DASVVN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424120"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF686_01495"
     CDS             complement(<1..208)
                     /locus_tag="VF686_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8660510.1"
                     /translation="MGLGIIGWIVIGIVAGWLAEKVMGRNHGLVTNLIVGVVGALLGG
                     FIAGQFLNMDVGGFNWMTLLVAFLG"
     gene            384..770
                     /locus_tag="VF686_01500"
     CDS             384..770
                     /locus_tag="VF686_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8660511.1"
                     /translation="MGAMATDIDLHLGRRLRRRRRLLGLTQQQLANQVGIRFQQIQKY
                     ECGANRISAARLWQLSEALESPVSYFYDGLEEAIERREAANQGGEMFSRKETLDLIQA
                     YYQLDEKPRRRLLDLAKSLHVEADAA"
     gene            912..1697
                     /gene="hisN"
                     /locus_tag="VF686_01505"
     CDS             912..1697
                     /gene="hisN"
                     /locus_tag="VF686_01505"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX8660512.1"
                     /translation="MTEFELFAVDLAREAARVSLPYFRGAYEESDKGGPGAFDPVTQA
                     DREAEAAIRTLIAARYPDHGVIGEEYGEDRPDADHVWILDPIDGTRAFIAGLPLWTNL
                     IALRTEGRPAVGVIGQPYLGEIFLGGPSGARLLKGGSETPLGVRRCASLNDAVIATTD
                     PDLFTGAELGAWTQVRAAARLARLGCDAYAYAMLAAGRIDLVAESALKVWDWAALVPV
                     IEAAGGAVSNWRGEAPDGSGQILAVGDIGIREQALVTLRRAAV"
     gene            complement(1694..>2277)
                     /locus_tag="VF686_01510"
     CDS             complement(1694..>2277)
                     /locus_tag="VF686_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8660513.1"
                     /translation="SGAILSSPMLRIKTGKRSMWSVKLAVRWNLRHGKAGDYVLDDAD
                     DPFDHTFGKDALTSDESRYEQWRQQLYACPHLAVGGPTWGWLAVGLDAGERALKPKAL
                     KAVQIPVAVVQAGEDDRVWKQTNKWAAKRMGRGRYVEIAGAKHEVIMETDDLRAVFLE
                     EFDAMAAYVSPVEDLSPMPTGAPVAAPEPTKAA"
BASE COUNT          359 a          749 c          806 g          363 t
ORIGIN      
        1 cgccgaggaa ggcgaccagc agggtcatcc agttgaagcc gccgacatcc atgttgagga
       61 actggccggc gataaagccg cccagcaatg cgccgacgac gccgacgatc aggttcgtca
      121 ccaggccgtg gttacggccc atgacttttt cagccagcca gccggcgacg atgccgatga
      181 cgatccagcc aatgattccc aagcccatct tgtatctccc ttgaagccca atgctctggc
      241 cgttaatgcg cgtgaaccgt tacggttgcg cgcggcccgc agaacgccgc tcaccgatac
      301 tcccccgtca cgcgagcgcg gcatggtgct tgccgcgtga ggggcgagcc gtcccgcttc
      361 gggacaggct gccaagatgg ggaatagggg ccatggccac cgacatcgac ctgcacctgg
      421 gacgccgcct gcgccgccgc cggcgtctgc tgggcctgac gcagcaacag ctggccaacc
      481 aggtcggcat ccgcttccag cagatccaga agtacgaatg cggcgccaac cgcatctcgg
      541 cggcccgcct gtggcagctg tccgaggcgc tggaatcgcc ggtcagttat ttctacgacg
      601 gtctggaaga ggccatcgag cgccgcgagg ccgccaatca gggcggagag atgttttccc
      661 gcaaggaaac gctcgacctg atccaggcct attaccagct ggacgaaaag ccgcgccgcc
      721 gcctgctgga tctggccaag tcgctgcacg tcgaggccga cgcggcgtag aagtggctag
      781 tgactagcgg ctagtggcta gtggctagtg attggcgctg cggcgcggcg ggcggaatga
      841 aaccgcagcc ggggctggcc ccgcgcgatc ctagccacta gtcactagcc actaaccact
      901 ggcttgcctc catgaccgag ttcgaactgt tcgccgtcga cctggcccgc gaggccgcgc
      961 gcgtgtccct gccgtatttc cggggtgcgt acgaagagag cgacaagggg ggaccggggg
     1021 cgttcgatcc cgtgacccag gccgaccgcg aggccgaggc ggcgatccgc acgctgatcg
     1081 cagcccgcta ccccgaccac ggcgtgatcg gggaagagta tggcgaggac cggccggatg
     1141 ccgaccacgt ctggattttg gaccccatcg acggcacgcg cgcctttatc gccggcctgc
     1201 cgctgtggac caatctgatc gctctgagga ccgaagggcg gccggccgtg ggcgtgatcg
     1261 gccagccgta tctgggtgag atcttcttgg gcgggccttc gggggcgcgg ctgttgaagg
     1321 gcgggtcgga gacgccgctg ggcgtgcgcc ggtgcgccag cctgaacgac gccgtcatcg
     1381 ccaccaccga tcccgacctg ttcacgggcg ccgagctggg cgcgtggacg caggtgcggg
     1441 cggccgcgcg tctggcccgg ctgggctgcg acgcctacgc ctatgcgatg ctggcggcgg
     1501 ggcggatcga tctggtcgcc gaaagcgcgt tgaaggtctg ggactgggcg gcgctggttc
     1561 cggtgatcga ggcggccggc ggcgcggtca gcaactggcg cggcgaggcg ccggacgggt
     1621 cagggcagat ccttgcggtc ggcgacatcg gtatccgcga gcaggcgctg gtgacgctga
     1681 ggcgggcggc ggtctaggcc gctttggtcg gctcgggcgc cgccacgggc gcgccggtcg
     1741 gcatgggcga caggtcttcg acgggcgaga cataggcggc catggcgtcg aattcctcga
     1801 ggaagaccgc gcgcaggtcg tcggtctcca tgatgacctc gtgcttcgcg cccgcgatct
     1861 cgacatagcg gccacggccc atccgtttgg cggcccattt gttggtctgt ttccagacgc
     1921 ggtcgtcctc gcccgcctga acgacggcca cgggaatctg caccgccttc agagccttgg
     1981 gcttgagcgc gcgctcgccc gcgtcgagtc cgacggccag ccagccccag gtcggaccgc
     2041 cgacggccag atgcgggcag gcgtagagct gctgccgcca ttgctcgtag cggctctcgt
     2101 cggacgtcag ggcgtccttg ccgaaggtgt ggtcgaacgg gtcgtcggcg tcgtccagca
     2161 catagtcgcc cgccttgccg tggcgcaggt tccagcggac cgccagcttg accgaccaca
     2221 tcgaccgctt gccggtcttg atgcgcagca tggggctgga caggatggcg cccgaga
//
LOCUS       DASVVN010000097         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_398444,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000097 DASVVN010000000
VERSION     DASVVN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398444"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VF686_01515"
     CDS             complement(<1..1456)
                     /locus_tag="VF686_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8660514.1"
                     /translation="MTQHRILLAAAVAALLTASPALAQNAEGARLNEHVRVLASDEFE
                     GRGVATPGEQKTVDYVVAQFQALGLEPGGPDGSWVQAAQLGRTRQDGPATITVSSGGA
                     TRALQRGPDVLVSSDRPVNRITVTDAAVVFAGYGVDAPERQWNDFKGVDLKGKVLLVI
                     VNDPDFGAPEGHPVNGRFDGNAMTFYGRWTYKFQEAARQGAAAVLVIHDTAGAGYGWS
                     VLEGSSTAPKFDIVRANPDAERAPAQGWIQGPVAEQMFADAGLDYAALREAARTPEFT
                     PVALDGVTMSIDFGQTADRIESRNILARIPGALHPGETVLYGAHWDAYGRATPVGDDD
                     IYNGAVDNATGVAGLIELARLFKAGTPPERSVVFAAWTAEEAGLLGAEYYAANPVWPL
                     ETTVANINMDSFLPGIEVSPQIVVMGAGKSQTDAWLERRATEQGRTLIPDPAPQAGGF
                     YRSDHFPLAKKGVPALFGAAGFTGHNQASRDYVAN"
     gene            complement(1525..2097)
                     /locus_tag="VF686_01520"
     CDS             complement(1525..2097)
                     /locus_tag="VF686_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660515.1"
                     /translation="MFSVQKITTAALIALAAWFAAFPGHAGPQDEPERTPREALALAA
                     GGDPVLHDAAMVKVLTNVAPTAVVTSPLHITGIAEGGLFHEGVFPIFLIGEEGCVLAQ
                     APARPLTDWMTGGEVAFEATVVFSAPPGSWATLVFEEDVEHANAPEPLDVRTRVTIAG
                     AEDPDYLVPRREDCIGYVGARLGPPPVPEE"
     gene            complement(2172..3377)
                     /locus_tag="VF686_01525"
     CDS             complement(2172..3377)
                     /locus_tag="VF686_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX8660516.1"
                     /translation="MSDVKTVHVIGAGPAGLMAAERLAQAGVRVVVHEAMPSVARKFL
                     MAGRGGLNLTHSEGLEGFVGRYGAASGTVAAWLERFSPTHLIGWAEGLGQATFTGSSG
                     RVFPRAMKASPLLRAWLARLDALGVEVRTRSRWTGRRDGAWVFGTPEGERVERPDAVV
                     LAMGGASWPRLGSDGACAAMLEGVETAPFRPSNVGFDVNWSDVFVERFAGQPLKPAAM
                     SLGDQTVRGEAMLTRYGIEGGAVYALSAGLRDAIERDGAAVLTVDLRPDLSVEALAGR
                     LAKPRGKDSVTNWLRKAGGLSAPAVGLLREIPGELPAGADKLAKRIKAVRLTLTGVQG
                     LARAISSAGGVKLDQLDDGLMLTAMPGVFVAGEMLDWEAPTGGYLLQASMASGVVAAD
                     GVMRWLAGR"
     gene            3427..3903
                     /gene="greA"
                     /locus_tag="VF686_01530"
     CDS             3427..3903
                     /gene="greA"
                     /locus_tag="VF686_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8660517.1"
                     /translation="MSVAFTREEDLEATAADLPDRPISPHPNLVTPSGLAAIEAALAS
                     ARAAYSAAQSKGSIEADRTAMARATRDLRYWSARRATAQLVETEPDGRVRFGGSVTIE
                     REDGRAQTWRITGEDEADPAAGSVSHVSPLAVALIGKTVGDEAVVAGQSVEIVAVD"
     gene            4065..4217
                     /locus_tag="VF686_01535"
     CDS             4065..4217
                     /locus_tag="VF686_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660518.1"
                     /translation="MARKPNYSFERQERDRADAKKAAEKAAAKAEKKAAAAARKEAGL
                     PPLEED"
BASE COUNT          717 a         1860 c         1398 g          666 t
ORIGIN      
        1 ggttggccac ataatcgcgg ctggcctgat tgtgccccgt gaaccccgcc gccccgaaca
       61 gggccggcac gcccttcttc gccagcggaa aatgatccga gcggtagaag ccgccggcct
      121 gcggggccgg atcggggatc agggtccggc cctgttccgt cgcccgccgc tccagccacg
      181 catccgtctg gctcttgccc gcccccatga ccacgatctg cggcgagacc tcgatgcccg
      241 gcaggaagct gtccatgttg atattggcca ccgtcgtctc cagcggccag accggattgg
      301 cggcgtaata ttcggccccc agcagcccgg cctcttccgc cgtccaggcc gcgaacacga
      361 ccgaccgctc gggcggtgtc cccgccttga acagccgcgc cagctcgatc agtccggcca
      421 cgcccgtcgc attgtcgacg gcgccgttat agatgtcgtc gtccccgacc ggcgtcgcgc
      481 ggccgtaggc gtcccagtgg gcgccataca gcaccgtctc cccggggtgc agcgctccgg
      541 ggatccgcgc cagaatgttg cggctttcga tccggtcggc ggtctggccg aagtcgatcg
      601 acatcgtcac cccgtccagc gctaccggcg tgaactccgg cgtccgcgcc gcctcgcgca
      661 gggccgcata atccagcccc gcgtcggcga acatctgctc ggctaccggt ccctggatcc
      721 acccctgcgc cggcgcccgc tcggcgtccg gattggcccg cacgatgtcg aacttcggcg
      781 ccgtcgacga tccctccagc acgctccagc catagccggc gcccgccgtg tcgtggatca
      841 ccagcaccgc cgccgccccc tgccgcgccg cctcctggaa tttgtaggtc cagcggccgt
      901 agaaggtcat ggcgttgccg tcgaaccgtc cgttcaccgg atgcccctcg ggcgcgccga
      961 agtccggatc gttgacgatg accagcagca ccttgccctt caggtccacg cccttgaagt
     1021 cgttccactg ccgctcgggc gcatcgacgc catagccggc gaagacgacg gctgcgtccg
     1081 tcaccgtgat ccggttcacc ggccggtcgc tggacaccag cacgtccggt ccgcgctgca
     1141 gcgcccgcgt ggcgccgccg ctcgacaccg tgatcgtcgc cggcccgtcc tgccgcgtcc
     1201 gccccagctg cgccgcctgc acccacgacc cgtccggccc gccgggctcc agccccagcg
     1261 cctggaactg cgccacgaca tagtcgaccg tcttctgctc gcccggcgtc gccaccccgc
     1321 gcccctcgaa ctcatccgag gccagcaccc gcacatgttc gttcagccgc gcgccctccg
     1381 cgttctgggc cagcgcaggc gaggcggtca ggagggccgc gacggcggcg gcgaggagga
     1441 tgcggtgctg agtcatgcaa tggttctacc gccttcgatc caggatcgtc accctcgcac
     1501 ctgaccccaa ggtcaggcgt tcagtcactc ctccggaacg ggcggcggtc caagccttgc
     1561 gccgacgtat cctatgcagt cttcgcgccg cggaacgagg tagtcggggt cctcggctcc
     1621 ggcgatcgtc acccgggttc gcacgtccag aggctccggc gcgttcgcgt gttcgacatc
     1681 ctcctcaaac acgagcgtcg cccatgatcc gggcggagcg ctgaacacca ccgtcgcttc
     1741 gaaagccact tcccctcccg tcatccagtc cgtcaacggt cgggcgggag cctgcgccag
     1801 aacgcagcct tcctcgccta tcagaaagat cggaaagacg ccttcgtgga atagtccacc
     1861 ctcggcgatg ccggtgatgt gcagcgggct ggtgacgacc gccgtgggcg cgacattggt
     1921 gaggaccttg accatcgcgg cgtcgtgcaa cacgggatcg ccgcccgccg cgagggccag
     1981 cgcctcccgg ggcgtccgct cgggctcgtc ctgcggaccg gcgtggccgg ggaaggcggc
     2041 gaaccacgcc gcgagggcga tcaaggcggc agtggtgatt ttctgaacgg agaacattgt
     2101 tttcctatgg cacaggcggc gcaacatttg tgctgaatta gaggggcctg cctgcgcccg
     2161 ctcgccgagc gtcaccgccc cgccagccac cgcatcacgc cgtccgccgc cacaaccccc
     2221 gacgccatcg aggcttgcag cagatagccg cccgtcggcg cctcccagtc cagcatctct
     2281 cccgccacga acacccccgg catggctgtc agcatcagcc cgtcgtccag ctggtccagc
     2341 ttcaccccgc ccgccgacga gatcgcccgc gccagcccct gcacgccggt cagcgtcagc
     2401 cgcaccgcct tgatccgttt cgccagcttg tcggctcccg ccggcagctc tccggggatc
     2461 tcccgcagca gccccaccgc cggcgccgac agtccgcccg ccttgcgcag ccagttggtg
     2521 acgctgtcct tgccgcgcgg cttcgccagc cgccccgcca gcgcctcgac cgacagatcg
     2581 ggccgcaggt ccacggtcag caccgccgct ccgtcccgct cgatcgcgtc cctcagcccc
     2641 gccgacagcg catagaccgc cccgccctcg atcccgtagc gcgtcagcat ggcctcgccg
     2701 cgcaccgtct gatccccgag gctcatcgct gccggtttca atggttgacc ggcgaaccgc
     2761 tccacgaaca catccgacca attcacgtcg aagccgacgt tcgaaggccg gaagggcgcc
     2821 gtctcgaccc cctccagcat cgccgcgcag gccccgtccg accccaaccg cggccagctg
     2881 gccccgccca tggccagcac cacagcatcg ggccgttcca cccgctcgcc ctccggcgtc
     2941 ccgaacaccc acgccccgtc gcggcggccg gtccagcgcg accgcgtccg cacctcgacg
     3001 cccagcgcgt ccagccgcgc cagccacgcc cgcagcagcg gcgaggcctt catcgcccgc
     3061 gggaacaccc gtcccgacga gcccgtgaac gtggcctgcc ccaacccctc ggcccagccg
     3121 atcagatgcg tcggcgagaa ccgttccagc caggccgcca cggtcccgct cgccgcgccg
     3181 tagcggccga caaagccttc cagcccctcc gaatgcgtca ggttcagccc gccgcgtccg
     3241 gccatcagga atttgcgcgc cacggagggc atggcctcgt gcaccaccac cctgaccccc
     3301 gcctgcgcca gccgctcggc cgccatcagc ccggccggcc cggccccgat cacatgcact
     3361 gtcttcacgt cgctcatcgc tccctgatgg accggaacgc cgcaagccgc catacgctca
     3421 tcccccatga gcgtcgcctt cacccgcgag gaggatctgg aggccacggc cgccgatctt
     3481 cccgaccgtc cgatctcccc gcaccccaac ctggtcacgc cctccggcct ggccgccatc
     3541 gaggccgccc tggcctcggc ccgcgcggca tactccgccg cccagtccaa aggctccatc
     3601 gaggccgacc gcaccgccat ggcccgcgcc acccgcgacc tgcgctactg gtcggcccgc
     3661 cgcgccaccg cccagctggt cgagaccgag cccgacggcc gcgtccgttt cggcggctcg
     3721 gtcaccatcg aacgcgagga cggccgggcc cagacctggc gcatcaccgg cgaggatgag
     3781 gccgatcccg ccgccggttc ggtcagccac gtctcacccc tcgcggtcgc tctgatcggc
     3841 aagaccgtcg gagacgaggc cgtcgtcgcc ggccagtcgg tggagatcgt cgccgtcgat
     3901 tgaacgctca atggttgccc cggctcgccg catgccccac cttgggaaca cagggcgctc
     3961 ccgcagcgct tactgcccgt agcgccgcgc cgtcgcgcca acaattcctg cgctcaagca
     4021 gcgcgacgcg cgatagcgcc aaaaagaaga gaaagaagcc gcccatggcc cgtaagccca
     4081 actacagttt cgagcgtcag gaacgcgatc gcgccgacgc caagaaggcc gccgaaaagg
     4141 ccgccgccaa ggctgaaaag aaggccgccg ccgccgcccg caaggaagcc ggcctgcccc
     4201 cgctcgaaga ggactgagca cagctccgtc tggaataaac cgtcgccttg tcatcccgga
     4261 cgccgcaagg cggcgatccg ggatgcccac attccaccga aacaaggcgc ccggaagctg
     4321 cgctggcagc tatccgggtg gcaggtaaac ctcagcgggg ctgcaacctg tctccctgac
     4381 aatcgctccg cgatttccgg gaggcccgat cgaggacagg attcgtcccg gatcgccttg
     4441 cggcatccgg gatgacaagg acctgcacct cgcagcggcg aagggcgtcc gcccctcttc
     4501 aggcccctgc cagactgcgc gacagtttgc gggccagctc ggcctgcgtg aacggcttgt
     4561 gcacgatcag gtcttcaccg acatcggtca gcgcttcgac gtcgacatag ccggtggcga
     4621 acagaatcgg cgcggacgga c
//
LOCUS       DASVVN010000098         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_292712,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000098 DASVVN010000000
VERSION     DASVVN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292712"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="tldD"
                     /locus_tag="VF686_01540"
     CDS             <1..1393
                     /gene="tldD"
                     /locus_tag="VF686_01540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX8660519.1"
                     /translation="ADVGADEALSILQGALEGADDGELFLERSESESLVFDDGRLKSA
                     AYDASEGFGLRVVAGETAGYAHANEISGPAIRRAADSASLAKSGHAGVTAEAPRATNQ
                     QLYDAVDPLASPAFSDKIALLSEIDAWARARDPRVVQVSASLVGERRQIEILRGDGRL
                     VRDLRPLVRMNVSITVESNGKRESASAGAGGRAGFETWIAPDRWQGQVDEALRQALVN
                     LEAVDCPAGEMDVVLGAGWPGVLLHEAIGHGFEGDFHRKGSSVFSGMMGKRVAAPGVT
                     VVDDGSIAGRRGSLSVDDEGTPTSRTVLIEDGIMVGLMHDRMSARQLGARATGNGRRQ
                     SFAHMPMPRMTNTFMEGGRDKQADMIASTKRGLYAANFGGGQVDITNGKFVFQCTEAY
                     LIEDGKITAPVRGATLIGDGATALQRVQMIGDDFAFDPGVGVCGKAGQGVPVGIGQPS
                     LKIAGLTVGGTAV"
BASE COUNT          268 a          633 c          576 g          250 t
ORIGIN      
        1 cgccgacgtc ggcgccgacg aagccctctc catcctgcaa ggcgcgctgg aaggggccga
       61 tgacggagaa ctgttcctcg aacgatccga gagcgaatcg ctggtcttcg acgacggccg
      121 gctgaaatcg gcggcctatg acgccagcga gggctttggt cttcgggtcg tggcgggcga
      181 gaccgccggc tacgcccacg ccaacgagat cagcggcccc gccatccgcc gcgccgccga
      241 cagcgcctcc ctcgccaaat ccggccacgc cggcgtcacg gccgaggccc cgcgcgccac
      301 caaccagcag ctttacgacg cggtcgaccc tctcgcctcg cccgccttct ccgacaagat
      361 cgccctgctg tccgagatcg acgcctgggc ccgcgcccgc gacccgcgcg tcgtgcaggt
      421 ctccgccagc ctggtcggcg agcgacgcca gatcgagatc ctgcgcggcg acggccggct
      481 ggtgcgcgac ctgcgtcccc tcgtccggat gaacgtttcc atcaccgttg aatccaacgg
      541 caagcgcgag agcgcctcgg ccggggccgg cggtcgcgcc ggcttcgaga cctggatcgc
      601 ccccgaccgc tggcagggcc aggtcgacga ggccctgcgt caggcgctgg tcaatctgga
      661 agccgtggac tgcccggctg gcgagatgga cgtggtcctg ggcgccggct ggcccggcgt
      721 gctgctgcac gaggcgatcg gtcacggctt cgagggcgat ttccaccgca agggctcctc
      781 ggtcttttcc ggcatgatgg gcaaacgggt cgcggctccg ggcgtcacag tcgtcgacga
      841 cggctccatc gccggccgcc gcggctcgct gtcggtcgac gacgagggca cgccgacgtc
      901 gcgcaccgtc ctgatcgagg acggcatcat ggtcgggctg atgcacgacc ggatgtcggc
      961 gcgtcagctg ggggcccgcg ccaccggcaa cggccggcgc cagtccttcg cccacatgcc
     1021 catgccgcgc atgaccaaca ccttcatgga aggcggcagg gacaagcagg ccgacatgat
     1081 cgcctcgacg aagcgcggcc tctacgccgc caatttcggc ggcggccagg tcgacatcac
     1141 caacggcaaa ttcgtcttcc agtgcaccga ggcctacctc atcgaggacg gcaagatcac
     1201 cgccccggtg cgtggcgcca ccctgatcgg cgacggcgcg accgcgctgc agcgggtgca
     1261 gatgatcggc gacgacttcg cttttgaccc tggcgtcggc gtctgcggca aggccggcca
     1321 aggggtcccc gtcggcatcg gccagcccag cctcaagatc gccggcctga cggtgggcgg
     1381 cacggcggtc tgatacgacc gcttctgacg catgccggct ctatgatgcc ggtcatgtcg
     1441 atcgctgccg aaaccccgcc agatgcgctg aacagactga gtagacgctg tagacgctgt
     1501 agactgtgtt tttcaggggg ccggccgcca ctccggcccg accacatcga acccgcgggc
     1561 ctcgagcccg tccagcacgc cgccggcttc ggccagaagg cgaatcgggg ccacgcccat
     1621 agtgactccg ggcgaggcga gcgcggtttc gatcgctgcc gcccactgct gcctcagctg
     1681 aggcgcaatt tccgggtccc cccaaggcca gcagcggtcc agcgcct
//
LOCUS       DASVVN010000099         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_476840,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000099 DASVVN010000000
VERSION     DASVVN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476840"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..958
                     /locus_tag="VF686_01545"
     CDS             2..958
                     /locus_tag="VF686_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8660520.1"
                     /translation="MPPVIEITGLTKTYKSGLQALKPIDLTIAKGEIFALLGPNGAGK
                     TTLISVVCGIVTPTTGTVVADGHDIQADYRAARSKIGLVPQELTTDAFETVLATVTFS
                     RGLFGKSPNPAFIEQVLRDLSLWEKRNDKIMTLSGGMKRRVMIAKALSHEPDILFLDE
                     PTAGVDVELRRDMWNLVRRLRETGVTIILTTHYIEEAEEMADRVGVILKGELILVEET
                     SALMKKLGKKTLTLNLQEPLAALPPELAEWSPTLKADGNELEYVFDSNLEKTGVPSLL
                     RKLSDLGIAFKDLHTSQSSLEDIFVSLVHRDKTDERKTGAAA"
     gene            964..1731
                     /locus_tag="VF686_01550"
     CDS             964..1731
                     /locus_tag="VF686_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8660521.1"
                     /translation="MTFNGHGVWAIYKFEMARAIRTVWQSVVTPVITTALYFVVFGSA
                     IGSRMTEIDGVPYGAFIVPGLIMLSLFTQSIFNASFGIYFPKFTGTIYELLSAPVSPL
                     EIVIAYVGAAATKSAVLGLIILATAAFFVPLQILHPLEMLAFLILISTTFSLFGFIIG
                     IWANGFEQLQMIPMLVVTPLTFLGGSFYSIDMLPPAWRTVTLFNPVVYLISGFRWTFY
                     GSGDVGIVASLAATLGFLFVCLAIVGWMFKTGYRLKN"
BASE COUNT          339 a          706 c          606 g          354 t
ORIGIN      
        1 catgcccccg gtcatcgaaa tcacaggcct gaccaagacc tacaagtccg ggctgcaggc
       61 cctcaaaccc atcgatctga cgatcgccaa gggcgagatt ttcgcccttc tcggccccaa
      121 cggcgccggc aagaccaccc tgatctccgt cgtctgcggc atcgtcaccc cgaccaccgg
      181 gaccgtcgtc gccgacggcc acgacatcca ggccgactac cgcgccgcgc ggtccaagat
      241 cggcctggtg ccccaggaac tgaccaccga cgccttcgag accgtgctgg ccacggtgac
      301 cttcagccgg ggcctgttcg gcaagtcgcc gaacccggcc ttcatcgaac aggtgctgcg
      361 cgacctcagc ctgtgggaga agcgcaacga caagatcatg accctgtccg gcggcatgaa
      421 gcgccgcgtc atgatcgcca aggcccttag ccacgagccg gacatcctgt tcctcgacga
      481 gccgacggcg ggcgtcgacg tcgaactgcg ccgcgacatg tggaacctgg tccgccgcct
      541 gcgcgagacc ggcgtcacca tcatcctgac cacccattat atcgaggagg ccgaggagat
      601 ggccgaccgc gtcggggtga tcctcaaggg cgagctgatc ctggtcgagg agacctcggc
      661 cctgatgaag aagctgggca agaagaccct gaccctcaat ctgcaggagc ccctggccgc
      721 cttgcctccg gaattggccg aatggtcgcc gacgctgaag gcggacggaa acgagctgga
      781 atacgttttc gattccaacc tcgagaagac cggcgtgccg tcgctgctgc gcaagctgtc
      841 ggacctcggc atcgccttca aggacctgca caccagccag agctcgctgg aggacatctt
      901 cgtcagcctc gtccatcgcg acaagacgga cgagcgcaag acgggagccg cggcatgagc
      961 agcatgacct tcaacgggca cggcgtctgg gccatctaca aattcgagat ggcgcgcgcc
     1021 atccgcaccg tctggcagtc ggtcgtcacg ccggtgatca ccacggccct gtatttcgtc
     1081 gtcttcggct cggccatcgg cagccggatg accgagatcg acggcgtgcc ctacggcgcc
     1141 ttcatcgtgc ccgggctgat catgctgagc ctgttcaccc agtcgatctt caacgcctcg
     1201 ttcggcatct acttccccaa attcaccggc accatctacg aactgctgtc cgcgcccgtg
     1261 tcgccgttgg agatcgtcat cgcctatgtc ggggcggcgg cgaccaagtc ggcggtgctg
     1321 gggctgatca tcctggcgac ggcggccttc ttcgtgccgc tgcagatcct gcatccgctc
     1381 gagatgctgg ccttcctgat cctgatctcg acgaccttca gcctgttcgg cttcatcatc
     1441 ggcatctggg ccaacggctt cgaacagctg cagatgatcc cgatgctggt ggtcacgccc
     1501 ctgaccttcc tgggcggctc cttctattcg atcgacatgc tgccgcccgc ttggcggacg
     1561 gtgaccctgt tcaatccggt ggtctatctg atctcgggct tccgctggac cttctacggt
     1621 tcgggggacg tgggcatcgt ggccagtctg gcggcgacgc tgggcttcct gttcgtctgt
     1681 ctcgccatcg tgggctggat gttcaaaacc ggctacaggc tcaagaacta gcccggacac
     1741 cggtctgaca cggcgtgtca cgaaacgggc catttcggcg gacaccctga ccgctgtagc
     1801 gttgatgaag cgcgcccgac ggggcgcggg agcctgaatg tccaccgagt ccgacaagcc
     1861 cctgaatgga acgcccgagc cgttccgccg ccccgtggtc tggggccgtc cgcctgcgac
     1921 ggtgttccgc gcgggtcccc tgcccagggg cgccgccctg ccgccgctgc cggaaccgcc
     1981 acgcgccccg ccgccccgtc cggcc
//
LOCUS       DASVVN010000100         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_135232,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000100 DASVVN010000000
VERSION     DASVVN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135232"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VF686_01555"
     CDS             <1..870
                     /locus_tag="VF686_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8660522.1"
                     /translation="ERRLLDPIRRAMDCFLACQQPAPQAGWGLQHHADTLKPAAARTY
                     EPEAYATHTTAANIGQLFSFYRLTGDRKYIARVPEALDWLESVRLPQRLQRDGRTHPT
                     FIQPGTNKPLYVHRRGSNVVNGAYYVDGNPEDTVIHYSSFRAVNVAGMRAEYDRLAAM
                     TPEAVREGSPLLARQGSVALPAYFTLQDISVSDMTSDGRRPPAEPAVVAGLIAGLNAR
                     GYWPTPLRAVSNPDIGDGSPEVAPGEFRITRVGDASDTSPYITDFPVMGVSTGEYIKN
                     MGVLIQALEGPPA"
     gene            1024..1099
                     /locus_tag="VF686_01560"
     tRNA            1024..1099
                     /locus_tag="VF686_01560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1057..1059,aa:Lys,seq:ctt)
     gene            1306..>1575
                     /locus_tag="VF686_01565"
     CDS             1306..>1575
                     /locus_tag="VF686_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660523.1"
                     /translation="MRTIAAALPATDPRREIFVGPTGKPRAELIQTTGYNDYENAKRI
                     ARDIQRRWAVDIAALRSHGNPRDADEALDRIDDWRSSALSGRRGST"
BASE COUNT          275 a          600 c          454 g          246 t
ORIGIN      
        1 gagcgccgtc tgctggatcc gatccgccgc gccatggact gcttcctggc ctgtcagcag
       61 cccgcgcccc aggccggctg gggtctccag caccacgccg acacgctcaa gcccgccgcg
      121 gcccgcacct atgagcccga ggcctacgcc acccacacca ccgccgccaa catcggccag
      181 ttgttcagct tctaccgcct gaccggcgac cggaaataca tcgcccgcgt gcccgaggcc
      241 ctggactggc tggagtccgt ccgcctgccc cagcggctgc agcgcgacgg tcgcacccat
      301 ccgaccttca tccagccggg cacgaacaag cccctctacg tccaccgccg cggctccaac
      361 gtcgtcaacg gcgcctacta tgtcgacggc aatcccgagg acaccgtcat ccactacagc
      421 tcgttccggg ccgtgaacgt cgccggcatg cgggccgagt acgaccggct cgccgccatg
      481 acgcccgagg ccgtgcgcga gggctcgccc ctgctggcgc gtcagggctc ggtcgccctg
      541 cccgcatact tcaccctgca ggacatctcg gtctcggaca tgaccagcga cggccggcgc
      601 ccccctgccg aacccgccgt ggtcgccggc ctgatcgccg gactgaacgc gcgcggctac
      661 tggcccacgc cgctgagggc ggtcagcaac ccagacatcg gcgacggctc gcccgaggtc
      721 gcccccggcg agttccgcat cacccgcgtc ggcgacgcca gcgacacctc cccctacatc
      781 acggacttcc cggtcatggg ggtctcgacc ggcgaataca tcaagaacat gggcgtgctg
      841 attcaagccc tggagggccc gcccgcctga attcgcgccc ctgcggcaaa ccgccggaca
      901 gacgcaaatc cccttgcgcg tgtgggacat tgcctctatg tccgccccct cgccgcgccc
      961 ggtccctgat cggcacagcg ggaacggcac gagccttgcg tggttctgcc cgacgaatgg
     1021 tccgggtgat tagctcagtt ggtagagcag ctgactctta atcagcgggt cgtaggttcg
     1081 aaccctacat cacccaccat cgtccttcga agctccagaa atcgctaaag ttcttgtccc
     1141 ggcaggattt cctgccgttc taccttatac aaatcttata caaaatgaga tcggtatgag
     1201 cagcaagagc cttggctttg ccagccccgc tacaagacgc cagatggtcg ttgggcggtc
     1261 gatgaggacg ccgtctacaa cgtccggcgc gtcatccccg ccgatgtgcg gaccattgcc
     1321 gccgcccttc ccgccaccga tcctcggcgt gaaatcttcg tcggccccac gggcaagccc
     1381 cgcgccgaac tgatccagac caccggctat aacgactacg agaacgccaa acgcatcgca
     1441 cgagacatcc agcgccgctg ggcggtcgac atcgccgcgc ttcgctccca cggaaacccg
     1501 cgagatgccg atgaggcgct ggatcggatc gacgactggc gctcgagcgc actctccggg
     1561 cgcaggggtt cgacg
//
LOCUS       DASVVN010000101         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_214205,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000101 DASVVN010000000
VERSION     DASVVN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214205"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VF686_01570"
     CDS             complement(<1..200)
                     /locus_tag="VF686_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660524.1"
                     /translation="MPMPTPELRAYAGRYDSRETGSPAEVMAADGGVAVRFGRGSVSR
                     LELAQVSRDVFRLGSMIARFER"
     gene            complement(547..>1530)
                     /locus_tag="VF686_01575"
     CDS             complement(547..>1530)
                     /locus_tag="VF686_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660525.1"
                     /translation="IAGNIRSIALPTLVTALVPEDRRDKANGLVGMVTGIGFLTTSVI
                     SGLLVAWGGMEGTLVFALGLTAPVFLHLRFMTVDEPDPEALRAAAGDHGPGRVDLKGT
                     MKVVGAVPGLFALILFATFNNFLGGVFMALMDAYGLSLMPVQAWGFMWAAVSTAFILS
                     GIVIAKTGLGKNPLRTLMIVNVACWAVAGVFTIQSSIVLLGVGCFLWMLLGPYAEAAE
                     HTTLQKVVPFERQGRVFGFAQSVEQAASPLTAFLIAPLAQFVFIPLMTDGWGADLIGA
                     WYGRGPERGIALVFTVAGVLGLVATVLAWNSSYYRRLSAAYAKAPAQEVGA"
BASE COUNT          272 a          571 c          496 g          191 t
ORIGIN      
        1 ttccgttcga accgcgcgat catcgacccc agccggaaga cgtcccgcga cacctgcgcc
       61 agttccagcc ggctcacgga gccccgcccg aaccgcacgg cgaccccccc gtccgccgcc
      121 atgacctcgg cgggcgagcc cgtttcgcgg ctgtcgtagc ggccggcata ggcgcgcagt
      181 tcgggggtgg gcatgggcat ggctgctccg gctggtggag cccgcactct gaccatcgaa
      241 tggctgagcg gcatgagcga accctgagca atcgctgaaa ccgtggatgg ccccatcagg
      301 ccgtgggcct tgcgcacttg tcatcccgga cgccgcgaag gcggcgatcc gggaggcttg
      361 agcgcggaaa catcatcgtc ccggaagctg cgtcggcagc tatccgggag aaggcccaat
      421 gcacaaagcg gcggctgccc ggctcccgga caatcgcgcc gcgatttccg ggaggcgccc
      481 gtcggtgagc gcaggccgcc ccggatcgcc gcgtccgcgg cgtccgggat gacaaggtgc
      541 aggagcctag gccccgacct cctgcgccgg cgccttcgca taggccgccg acagccggcg
      601 ataatagctg gagttccacg ccagcaccgt cgcgaccagc cccagcaccc cggccacggt
      661 gaacaccagt gcaatgcccc gctcgggccc gcgtccgtac caggcgccga tcaggtccgc
      721 gccccagccg tcggtcatca gcggaatgaa gacgaactgc gccagcggcg cgatcaggaa
      781 ggccgtcaac ggcgacgccg cctgctcgac cgactgggca aacccgaaca cccgcccctg
      841 ccgctcgaac ggcaccacct tctgcagggt ggtgtgctcg gccgcctcgg cataggggcc
      901 cagcagcatc cacaggaaac agcccacgcc cagcagcacg atcgacgact ggatggtgaa
      961 cacccccgcc acggcccagc aggcgacgtt gacgatcatc agggtgcgca gcgggttctt
     1021 gcccagcccc gtcttcgcga tgacgatccc gctgaggatg aaggccgtcg acacggcggc
     1081 ccacatgaag ccccacgcct gcaccggcat gagcgacagg ccataggcgt ccatcagcgc
     1141 catgaagacg ccgccgagga agttgttgaa ggtcgcgaac aggatcaggg cgaacaggcc
     1201 gggcacggcg cccacgacct tcatcgtccc cttcagatcc acccgcccgg gcccgtgatc
     1261 ccccgccgcc gcccgcagcg cctcgggatc gggctcgtcc acggtcatga agcgcagatg
     1321 caggaacacc ggcgcggtca ggcccagggc gaagaccagc gtcccctcca tcccgcccca
     1381 tgcgaccagc aggccgctga tcaccgaggt ggtcagaaag ccgatcccgg tcaccatccc
     1441 gaccagcccg ttggccttgt cgcgccggtc ctcgggcacc agggccgtga ccagcgtcgg
     1501 cagggcgatg gagcggatgt tgccggcgat
//
LOCUS       DASVVN010000102         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_345930,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000102 DASVVN010000000
VERSION     DASVVN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345930"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF686_01580"
     CDS             <1..459
                     /locus_tag="VF686_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8660526.1"
                     /translation="PWTVRILSDQPRLTSYSGVAIEAHGALAEAAEADAVFVVSGDGS
                     RAKLADPAFMAALQLDPSRQVIGGIDSGALILAGLGLLEGLSATTYPPVMPELEAMGV
                     RTEMRALVVHGNVATAGGCLASLDLCAWMVGRLLDPEAAARVRSSFDPNS"
     gene            593..1582
                     /locus_tag="VF686_01585"
     CDS             593..1582
                     /locus_tag="VF686_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX8660527.1"
                     /translation="MKLFSGLSAFPITPMTSAGEVIVPDLQGLVRRIEAGGADSIGLL
                     GSTGTYMFLTRDERRRAVAAAVGSAASTPVIVGVGALRTDEARALARDAAEEGAAGLL
                     LAPVSYTPLTEDEVFQHFCAVASATSLPLCIYSNPGTTNFTFSPALVGRLAAVPTIAA
                     IKLPLPAGDVASDLGDFRRAAPGLSIGYSGDWGCKDALLAGADGWFSVVGGLFPERAA
                     ALTAAAMANDRAGADAHNEAFAPLWGLCKAVGSVRVMYAAANAMGLTRAQPPRPLLGL
                     SPADQETLAALLDSPPQTRALDLSASAAGRQGRVLACGGAAPGIMYQGVGPGS"
     gene            complement(1510..2382)
                     /locus_tag="VF686_01590"
     CDS             complement(1510..2382)
                     /locus_tag="VF686_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8660528.1"
                     /translation="MSAPSYAQVLTPPPVEVGAPIQSGTSYTLRSDVLGDDRQINVWL
                     PPDYSGSDASYTVLYLLDGALDQDFHHIAGLAQLGSLSWTYGPLIVVGIQTKDRRAEL
                     TPQASDPRYLQAFPQSGGAERFRRFLETEVLPFVEGRFRVGPRRALMGESLGGLFVVD
                     TLLVQPSLFNDYVAISPSLWWYDRLLLKGAASRLRAADGAGRRLYLAMADEGGTMQQG
                     VDLLREALGDSRAETIEWRYSDRSSVATHATVYHSAAEEALRWLYPAPPYDPGPTPWY
                     MIPGAAPPQASTRP"
     gene            complement(2485..>3018)
                     /locus_tag="VF686_01595"
     CDS             complement(2485..>3018)
                     /locus_tag="VF686_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8660529.1"
                     /translation="AVGMAAMLALPSVILMMIYGQTRIFFVMARDGLLPRSFAKIHSK
                     WKTPYIVTLFTGACVAIAGAFFPVGQLADISNSGTLFAFFMVSIAVMVLRVKDPNRRR
                     PFRTPFLWVIAPISMLGCVFLFWNLPHDARMVLPIWGGLGLVVYLLYGYRNSHLGRGV
                     VEPAGEPLPGPKPPGAA"
BASE COUNT          495 a          997 c         1055 g          471 t
ORIGIN      
        1 ccctggacgg tccgcatcct gtccgaccaa ccgcggctga cgtcttattc cggcgtcgcc
       61 atcgaggcgc acggcgcgct tgccgaagcg gcggaggcgg atgcggtgtt cgtggtcagt
      121 ggggacggca gccgggccaa gctcgccgat cccgccttca tggcggccct gcagctggac
      181 ccctcccgtc aggtgatcgg cggcatcgat tccggcgcgc tcattctggc gggtctcggt
      241 ctgttggagg gactgtccgc cacgacctac ccgccggtca tgccggaact cgaagcgatg
      301 ggcgttcgca cggaaatgcg ggccctggtc gtgcacggca atgtcgcaac cgcgggcggc
      361 tgcctggcat cgctcgacct gtgcgcctgg atggtcgggc ggctgcttga ccccgaggcg
      421 gcggcccgcg tgcgatcgtc cttcgatccg aatagctgag cggcgcccgg tcagaagatt
      481 ggcggcgaca ggacgccgca gccgggcggc gagccgcgtt acaaatcgaa gccgatgatg
      541 atttgaatcg cgccccggat cagggctgat cgggcgacct gcggagccgg gcatgaagct
      601 gttctccggc ctttcggcct ttccgatcac accgatgaca agcgcgggcg aagtcatcgt
      661 ccccgatctg caggggctgg tccgccggat cgaggcgggc ggcgcggact cgatcgggct
      721 gctgggcagc accgggacct atatgttcct gacgcgggat gagcgccggc gcgctgtcgc
      781 cgccgccgtc gggagcgcgg catcgacacc cgtgatcgtc ggcgtcggcg cattgcggac
      841 cgacgaagcc cgggcgcttg cccgggatgc agcggaagaa ggcgctgcag ggctgttgct
      901 ggcccccgtc agctacacgc cgctcacgga ggacgaggtc tttcagcatt tctgcgcggt
      961 cgcgtcagcg acctcgctgc ccctgtgcat ctacagcaat ccgggaacga ccaacttcac
     1021 cttcagcccc gctctcgtcg gccgcctggc ggctgtcccg accatagcgg cgatcaaact
     1081 gccactccct gcgggcgacg tcgcctcgga tttgggcgac tttcgccgcg cggcgcctgg
     1141 actgtcgatc ggctatagcg gcgactgggg ctgcaaggac gccttgctgg ccggcgcaga
     1201 cggctggttc agcgtcgtcg gcggcctgtt ccccgagcgc gccgccgccc tcacagccgc
     1261 ggcgatggcg aacgaccggg cgggggccga cgcccacaac gaagccttcg cgccgctttg
     1321 gggactgtgc aaagcggtcg gcagcgttcg cgtgatgtat gcggcggcca acgccatggg
     1381 attgacacgg gcccagcctc ctcgcccgtt gctgggcctg tctccggccg atcaggaaac
     1441 gctcgcggcg cttctagaca gcccgccgca aacgcgggcg ctggacctct cagcctccgc
     1501 cgcagggcgt cagggccggg tgctcgcctg cggcggcgcc gcgccgggga tcatgtacca
     1561 gggcgtgggg cccggatcat agggcggggc cgggtaaagc cagcggagcg cctcttcggc
     1621 ggcgctgtga tagaccgtgg cgtgggtggc gaccgagctt cggtccgagt agcgccactc
     1681 gatggtctcc gcccgggagt cgcccagggc ctcccgcagc aggtccacgc cttgctgcat
     1741 cgtcccgcct tcgtccgcca ttgccagata gagcctgcgg cctgcgccgt cggcggcgcg
     1801 cagccggctg gccgccccct tcagcagcag gcggtcgtac caccacaggc tcggactgat
     1861 cgccacatag tcattgaaga gcgagggctg gaccaggagc gtgtcgacca cgaacaggcc
     1921 gcccagggac tcgcccatga gcgcccgacg gggaccgacc cgaaagcggc cttccacaaa
     1981 cggcagcacc tcggtttcga ggaaccgccg gaagcgctct gcgcctccgc tctgcggaaa
     2041 ggcttgcaga tagcgagggt ctgacgcctg cggggtgagc tccgcccgcc ggtccttcgt
     2101 ctgaatgccc acgacgatga gcggtccata tgtccaactt aacgacccga gctgagccaa
     2161 acctgcgatg tgatggaagt cctgatccag cgccccatcg agcaggtaga gcaccgtata
     2221 gctggcatca gaccccgaat aatccggcgg gagccagacg ttgatctggc ggtcatcgcc
     2281 gaggacatcc gagcggagcg tgtaggatgt tccgctctgg atcggcgcgc cgacctcgac
     2341 cggaggcggg gtcaggacct gagcgtagct tggcgctgac aagagcggcc aggcgatggc
     2401 cgcgatgagc gcgccccagg cccttcgccg tccgcggcgc gaggtgggcg ttccaggagt
     2461 tcgtctagcc actcggtgat cctcctaagc ggcgccaggg ggtttggggc cgggcaaggg
     2521 ctcgcctgcg ggttcgacga cgcctcggcc cagatggctg ttccggtagc cgtagaggag
     2581 gtagacgacg agccccaacc cgccccagat cgggagcacc atcctggcgt cgtggggcag
     2641 gttccagaac aggaacacgc atcccagcat cgagatcggg gcgatgaccc agaggaaggg
     2701 ggtgcggaat ggtcgacgac ggttcgggtc tttcacacgc agaaccatca cggcgatcga
     2761 gaccataaag aaggcgaaca gcgtgccgga gttcgaaatg tctgcaagct gaccgaccgg
     2821 gaagaaggcc cctgcgatgg cgacgcaggc cccggtgaac agggtcacga tatagggcgt
     2881 tttccatttg gagtggatct tcgcgaagct cctgggcagg agaccgtcgc gggccattac
     2941 gaagaaaatg cgcgtctggc cgtagatcat catcaggatg accgacggca aggccagcat
     3001 ggcggccatg ccgaccgc
//
LOCUS       DASVVN010000103         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_29655, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000103 DASVVN010000000
VERSION     DASVVN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29655"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF686_01600"
     CDS             complement(<1..392)
                     /locus_tag="VF686_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX8660530.1"
                     /translation="MNRWSPQPAPDEADGLILFDGVCVFCSRWVRFVIDHDPERRFSF
                     APIQSEAGQALAARYGIDPEAPQTNAVVWDGRIYFKSDAALTVLGQLSRTRALAGLKA
                     LPRLLRDPAYDLIARNRYAIFGRTETCMV"
     gene            complement(435..824)
                     /locus_tag="VF686_01605"
     CDS             complement(435..824)
                     /locus_tag="VF686_01605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8660531.1"
                     /translation="MAAIIRPLTGDGSPPAEGAYAQAAEVTGATRWLYLSGQIPVAPD
                     GSLAPDFIGQCEQVWDNVETQLKAAGMTLDNLVKVTTFLSDRAHALENREVRIRRLGG
                     RQTALTVIVTGIFDEAWLVEIEAVAAA"
     gene            complement(838..2463)
                     /locus_tag="VF686_01610"
     CDS             complement(838..2463)
                     /locus_tag="VF686_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8660532.1"
                     /translation="MFRKMMGGVAVAAVMLAAGMASAQDFSATRISDNIRTISADEYQ
                     GRYPGTEGERMVLSWLQAQYEAMGFEPGGPNGQWLQPVELKRYTPVAGATASWTGPDG
                     QVHALTPGTDIVLRAVTNDGRASIRDAGVVFVGHGIYAPERQWDDYGDTDVRGKVVVV
                     VGGEPDGERFNGEYSTNYESGAYKADEAFRRGAVGIITLVAQPASSPMWQGAIRGAGR
                     TRTLTPGAADLEFSGAMPHDTAAAWAAAAGLDISQLPNIADGSFKAVALPGVALSVEV
                     AETIETLTTHNLLARSPGTEHPEETIIYSAHWDHVGGEAQHPNAPGDDKIWNGAWDNA
                     SGTIGVLEMARQIKAAPVPERSIVFAHMAAEEMGLLGAYGYAANPVWPLETTVADINI
                     DMLPLSGSTRDIPIFGKGQNDLEDRLEALAAPLGRYVSDDHMPEEGFYYRSDHFPFAR
                     MGVPAIMPWHGWDWVQGGREAGEAAWRAKFSADYHKPSDEWSADWDLTSAVENLTLLY
                     HLGLDLANSSDWPGWKPTSEFAVVRARSDAARQ"
     gene            complement(2805..>3337)
                     /locus_tag="VF686_01615"
     CDS             complement(2805..>3337)
                     /locus_tag="VF686_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8660533.1"
                     /translation="ESGLLGSEFYAANPVYPLATTVAGFNIDAMNVYGRVADVDIIGS
                     GQSNLDARVTAEATRQGRNAKPDGNPASGSYFRSDHFPLAKRGVPMAYVDGGGAFRDE
                     PIAAREAARSEYGSRRYHQADDEWSADWDLRGQVEDLELIFNIGRDLANSRDWPEWMP
                     GSEFGPARTPSAGERR"
BASE COUNT          505 a         1168 c         1132 g          532 t
ORIGIN      
        1 accatgcagg tctccgtccg gccgaagatc gcataccggt tccgcgcgat caggtcatag
       61 gccgggtcgc gcagcagtcg cggcagggcc ttcagccccg ccagcgcccg cgtcctggac
      121 agctgcccca gcacggtcag ggccgcatcc gacttgaagt agatccgccc gtcccacacc
      181 acggcgttgg tctgcggcgc ctccgggtcg atcccgtacc gcgccgccag cgcctgcccc
      241 gcctccgact ggataggggc gaaactgaac cgccgctccg ggtcgtggtc gatcacgaac
      301 cgcacccagc gcgagcagaa cacgcacacc ccgtcgaaca ggatcaggcc gtcggcttca
      361 tccggggcgg gctgcggcga ccagcggttc atgcgagcac actgcggcgc ttgcaggggc
      421 cgcacaaggc gacgttacgc cgccgccacc gcctcgatct cgaccagcca ggcctcgtcg
      481 aagatgccgg tgacgatcac ggtcagggcc gtctggcggc cgcccagccg gcggatgcga
      541 acctcacggt tctccagcgc atgggcccgg tcagacagga aggtcgtcac cttgaccaga
      601 ttgtccagcg tcatccccgc ggccttcagc tgggtctcga cattgtccca gacctgctcg
      661 cactggccga tgaagtcagg agccagcgag ccgtcgggcg cgaccggaat ctggccggac
      721 agatagagcc accgggtcgc cccggtgacc tccgccgcct gcgcataggc gccctccgcg
      781 ggtggagagc cgtctccggt cagcggtcgg atgatcgcgg ccatcgcccc gcccgcccta
      841 ttggcgcgcg gcgtccgagc gggcgcgcac gacggcgaat tccgacgtcg gcttccagcc
      901 cggccagtcg ctgctgttcg cgagatccag acccagatga tacagcagcg tcaggttctc
      961 gaccgccgag gtcaggtccc agtccgccga ccattcgtcc gacggcttgt gatagtcggc
     1021 cgagaatttg gcgcgccacg cggcctcgcc cgcctcacga ccgccttgga cccagtccca
     1081 gccgtgccac ggcatgatcg ccggcacgcc catgcgggcg aacgggaagt ggtccgagcg
     1141 atagtagaag ccttcctcgg gcatgtggtc gtccgagaca tagcggccca gcggcgccgc
     1201 cagggcttcc agccggtcct ccagatcgtt ctggcccttg ccgaagatcg ggatgtcgcg
     1261 ggtcgagccc gacagcggca gcatgtcgat attgatgtcg gcgacggtcg tctccagcgg
     1321 ccagaccgga ttggcggcgt agccgtaggc gcccagcaag cccatctcct cggccgccat
     1381 atgggcgaag acgatcgagc gttcggggac cggggcggcc ttgatctgac gcgccatctc
     1441 cagcacgccg atggtgcccg aggcgttgtc ccacgcgccg ttccagatct tgtcgtcgcc
     1501 gggggcgttc gggtgctggg cctcgccgcc gacgtgatcc cagtgggccg aatagatgat
     1561 ggtctcttcc ggatgctcgg tcccgggact gcgggccagg aggttgtggg tcgtcagggt
     1621 ttcgatggtt tcggcgacct cgaccgacag ggcgacgccc ggcagggcca cggccttgaa
     1681 cgacccgtcg gcgatattcg gcaactggct gatgtccaga ccggctgcgg cggcccatgc
     1741 ggcagccgtg tcatgcggca tggcgccaga gaactcgaga tcggctgcgc ccggcgtcag
     1801 ggtgcgggtg cggccggcgc cccggatcgc gccctgccac ataggcgacg acgcgggttg
     1861 ggcgaccagg gtgatgatgc cgaccgcgcc gcggcggaac gcctcgtccg ccttgtaggc
     1921 gccggactca tagttggtcg aatactcgcc gttgaaccgc tcgccgtcgg gctcgccgcc
     1981 gaccaccacc acgaccttgc cgcgcacgtc ggtgtcgccg tagtcgtccc actggcgctc
     2041 cggggcgtag atgccgtggc cgacgaagac cacgcccgcg tcccggatcg aggcgcggcc
     2101 gtcattggtc acggcgcgca ggacgatgtc ggttcccggc gtcagcgcgt ggacctgtcc
     2161 gtccggaccg gtccagctgg ccgtcgcgcc ggcgacgggc gtgtagcgtt tcagttcgac
     2221 gggttgcagc cactggccgt tcgggccgcc cggctcgaag cccatggctt cgtactgggc
     2281 ctgcagccag gacagcacca tccgctcgcc ctcggtcccg ggataacggc cctggtactc
     2341 gtcggcgctg atggtgcgga tgttgtcgct gatccgggtg gccgagaagt cctgggccga
     2401 ggccatgccg gcggccagca tcacggcggc gacggcgacg ccgcccatca tcttgcgaaa
     2461 cattggtgtg gctccatccc ggatttgatg gcccgcactc tagggagacg ccctgacggc
     2521 gtccgttaag ttttcgtcat gtcagcatgc agggaggcaa gatgccaagg ctgggccctt
     2581 gtcatcccgg acgccgcgca gcggcgagcc gggacgatat gcagcactgg ctgatgactc
     2641 ccggaaatcg cccagcgatt gtccggggga aagggccccc gtcgcggcgc cctgacccgt
     2701 ctcccggatc gctgctcacg cagcttccgg gagatcagga ttttgcattg caagcgtccc
     2761 ggatcgccgc gttcgcggcg tccgggatga caaggagctc tgccctaccg ccgctcaccc
     2821 gcgctcggcg tacgcgccgg gccgaactcc gaacccggca tccattccgg ccagtcgcgg
     2881 ctgttcgcca gatcgcggcc gatgttgaag atcagctcca gatcctcgac ctgaccgcgc
     2941 aggtcccagt cggccgacca ttcgtcgtcg gcctggtgat agcggcgcga accgtattcc
     3001 gaacgggccg cttcgcgcgc tgcgatcggc tcgtcgcgga aggcgccgcc gccgtcgaca
     3061 taggccatcg gcacgccgcg cttggccagc gggaagtggt ccgagcggaa atagctgccc
     3121 gaggccggat tgccgtccgg cttggcgttg cgaccctgac gcgtcgcctc ggcggtgaca
     3181 cgggcgtcga ggttcgactg gcccgagccg atgatgtcca cgtcggcgac gcggccatag
     3241 acgttcatgg cgtcgatgtt gaagcccgcc acagtcgtgg ccagcggata gaccggattg
     3301 gcggcgtaga attccgaacc cagcaggccg ctctcct
//
LOCUS       DASVVN010000104         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_503743,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000104 DASVVN010000000
VERSION     DASVVN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503743"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VF686_01620"
     CDS             <1..551
                     /locus_tag="VF686_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8660534.1"
                     /translation="TAAPIDMSVFRFLKALADLDKPAVAAVRGRAVGIGLTLLLHCDM
                     VVVAEDALLSAPFINLALTPEAASSLLLPAVLGHQRAFEIFALGEPIDGRTALAWGLA
                     NRAVPTDQVEAVAAELAGKLATRAPNSIRKTKALMRDSERLWALMQREGEAFGSQMKS
                     PEAMEAFMAFSQKRAPDFSKAG"
     gene            574..1065
                     /locus_tag="VF686_01625"
     CDS             574..1065
                     /locus_tag="VF686_01625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX8660535.1"
                     /translation="MRRLMFDPLPEPVRIEAETSADAAAVDALVLAAFGPGRFAKTAE
                     RLREHAGVAAGFVAREEGEVIGSVRLWRITVGETPALFLGPIAVAVDSRKGGLGADLV
                     QACVDHAAHEGVAILLVGDMGYFQRFGFAVASDVQLPGPVDRARVLWRGEGSPTGLVR
                     PIR"
     gene            1108..1689
                     /locus_tag="VF686_01630"
     CDS             1108..1689
                     /locus_tag="VF686_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX8660536.1"
                     /translation="MASTADNRTGLAGVAEAAKQAPRSSEGPRGLPPVHLWHPAHCGE
                     IDIVIRRDGVWMHEGSPIGRAELVRLFSTVLRKDEDGYVLVTPGEKLSITVEDLPFRA
                     VTVERRGEGLVFTTDVGDEAEAGPEHPIVVETDPETGEPSPSVRVRADLWARIARSVF
                     YELVEMADVEDGRLTVRSNGAPFALGPVGAGVE"
     gene            1686..2348
                     /locus_tag="VF686_01635"
     CDS             1686..2348
                     /locus_tag="VF686_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX8660537.1"
                     /translation="MSLSTLRARLIERLEPASGWLPDAAATRSDFDLNPGATRPEQTL
                     RPAAVLIPVIAGPEGATVLLTRRADSLTSHTGQIAFPGGRLDPGETAVEAALREAMEE
                     VALDPSKVEVLGVGDAYETGTGFLITPVIGWLTEPPQTTPAPAEVAEVFEVPWDFLMD
                     IANHRREAWDREGQPRRWYWAMPYGERYIWGATAGVIRSLHARLYDCEAAPDAAAAED
                     AA"
     gene            2345..>2604
                     /locus_tag="VF686_01640"
     CDS             2345..>2604
                     /locus_tag="VF686_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX8660538.1"
                     /translation="MTVRLTDQPWLTASSTRAVMAALEAAGGPGCARFVGGCVRNALI
                     GAPVDDIDIATTLKPEDTDKAIRAAGLKAVPTGIAHGTVTAVT"
BASE COUNT          354 a          869 c          987 g          394 t
ORIGIN      
        1 agaccgccgc gcccatcgac atgtccgtct tccggttcct gaaggccctg gccgacctgg
       61 acaagccggc cgtggccgcg gtgcgtggaa gggccgtggg catcggcctg acgctgctgc
      121 tccactgcga catggtggtg gtggccgagg acgccctcct gtcggcgccc ttcatcaatc
      181 tggccctgac gccggaggcg gcttcgtcgc tgcttctgcc ggcggtgctg ggccatcagc
      241 gggcgttcga gattttcgcc ctgggcgaac cgatcgacgg gcgcacggcc ctcgcctggg
      301 gcctggccaa ccgggcggtc ccgaccgatc aggtcgaggc cgtggcggcg gaactggccg
      361 gcaagctggc gacgcgggcg cccaattcga tccgcaagac caaggcgctg atgcgcgact
      421 cggaacgcct gtgggcgctg atgcagcgcg agggcgaggc cttcggcagc cagatgaaga
      481 gccccgaggc gatggaggcc ttcatggcct tcagccagaa gcgcgcgccc gatttctcga
      541 aagccggttg attcggcggg tcgggggccc tagttgcgcc gcctgatgtt cgaccccctg
      601 cctgaacccg tgcgcatcga agccgaaacc tccgccgacg ccgcggcggt cgacgccttg
      661 gtgctggccg cctttggtcc gggccgcttc gccaagacgg ccgagcggct gcgcgaacat
      721 gccggggtgg ccgcgggctt tgtcgcgcgc gaagagggcg aggtcatcgg ctcggtgcgc
      781 ctgtggcgga ttacggtcgg cgagacgcca gccttgtttc tcgggcctat cgccgtggcc
      841 gtggacagcc gcaagggcgg gctgggcgcc gatctggtgc aggcttgcgt cgatcatgcg
      901 gcccacgagg gtgtcgctat cctgctggtc ggagacatgg gctatttcca gcggttcggc
      961 ttcgcggtcg cgtcggacgt acagctgccg gggccggtgg accgggcgcg cgtcctgtgg
     1021 cgcggcgagg gttcgccgac gggtctggtc cggcccatcc gctagaaggg gtttgccgcc
     1081 gtcgctccga cgcctaagtc tgaacccatg gcatccaccg ccgacaacag gaccgggctg
     1141 gccggggtcg ccgaggcggc gaaacaggcg ccccgttcca gcgaggggcc ccgtggcctg
     1201 ccgcccgtgc atctgtggca tccggcgcat tgcggcgaga tcgacatcgt catccgccgc
     1261 gacggggtct ggatgcacga aggctcgccc atcggccggg ccgagctggt gcggctgttc
     1321 tcgaccgtgc tgcgcaagga cgaggacggc tatgtgctgg tcacgccggg cgaaaaactg
     1381 tcgatcacgg ttgaggatct gccgttccgg gcggtgacgg tggaacggcg cggcgagggg
     1441 ctggtcttca ccaccgacgt cggcgacgag gccgaggccg ggcccgaaca tcccattgtc
     1501 gtggaaaccg atccggagac gggcgagcct tcaccctcgg tgcgggtgcg cgcggatctg
     1561 tgggcgcgga tcgcgcggtc ggtcttctac gaactggtcg agatggcgga tgtcgaggac
     1621 gggcggctga cggtgcgctc gaacggcgcg cccttcgccc tggggcccgt cggggcgggc
     1681 gtggaatgag cctgtccacc ctgcgcgcgc ggctgatcga gcggctggaa ccggcctcgg
     1741 gctggttgcc ggacgcggcc gccacgcgtt ccgatttcga tctgaatccc ggtgcgacgc
     1801 ggcccgagca aacgctgcgg ccagctgcgg tgctgatccc ggtcatcgcg ggaccggagg
     1861 gcgcgacggt gctgctgacc cgccgcgccg acagcctgac cagtcatacg ggccagatcg
     1921 cctttcccgg cggccggctg gaccccggcg agaccgccgt cgaggccgcg ttgcgcgagg
     1981 cgatggagga ggtggcgctg gacccctcca aggtcgaggt gctcggcgtc ggcgacgcct
     2041 atgagaccgg cacggggttc ctgatcacgc cggtgatcgg ctggctgacc gagccgcccc
     2101 aaaccacgcc cgctccggcc gaggtcgcgg aggtgttcga ggttccgtgg gacttcctga
     2161 tggacatcgc caaccaccgc cgggaagcgt gggaccggga gggccagccg cgccgctggt
     2221 actgggccat gccctatggc gagcgctaca tctggggcgc gacggcgggc gtcatccgat
     2281 ccctgcacgc gcggctgtac gattgcgagg ctgcgcccga cgcggcggcg gcggaggatg
     2341 cggcatgacc gtccggctga ccgaccagcc ctggctgacg gcctcgtcga cacgggcggt
     2401 gatggcggcg ctggaggcgg cgggcggacc cggctgcgcc cggttcgtcg gcggctgcgt
     2461 gcgcaacgcc ctgatcggcg cgccggtgga cgacatcgac atcgccacga cgctgaagcc
     2521 cgaggacacg gacaaggcga tccgcgccgc cggcctgaag gccgtgccga cgggtatcgc
     2581 ccatggcacc gtgacagcgg tgac
//
LOCUS       DASVVN010000105         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_3763, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000105 DASVVN010000000
VERSION     DASVVN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VF686_01645"
     CDS             complement(<1..1133)
                     /locus_tag="VF686_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX8660539.1"
                     /translation="MADGTGAPAFLGVSRSLSGRAWRQRPAEAGLIRSHMQTLGLEEP
                     LARALAARGVRADQGADFLQPTLKALFPDPSSFMDMDAAAGAILDALQAQASIHVFAD
                     YDVDGASSAALLVRWFRAMGADLPIYVPDRMTEGYGPSAKAFDTLKASGADLVITVDC
                     GAAANEAVAHAAEIGLKVVVIDHHMMREHPPVCAAVVNPNRPGCNSGQGNLAAAGVVF
                     VLLAALNREARGRGLFEGREQPDIRQWLDLAAMGAICDVTGLTGFNRALTSLGLRRMS
                     EWANPGLRALLAAAGAEPGPAKSNHAGFILGPRINAGGRIGKSDLGARLLSTDDPVEA
                     AALAQELDALNVARREVEKQVTDQATRMVEATGAHADGSAVVV"
     gene            complement(1133..2089)
                     /gene="glpX"
                     /locus_tag="VF686_01650"
     CDS             complement(1133..2089)
                     /gene="glpX"
                     /locus_tag="VF686_01650"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX8660540.1"
                     /translation="MAASAPYASTLAADAARVTELAAIASYAQIGRGDEKQADQLAVD
                     AMRTALNNLDIDGKIVIGEGERDEAPMLYIGEKVGTGKGPAIDIALDPLEGTTLTAKA
                     MANALCVLSMSPGGGMLHAPDTYMDKIAIGPGFAPGTVDLDMKPQDNVRSLASAKGVK
                     PEDITVCVMDRPRHADLIAALREVGARVLLITDGDVAGVIHTAEPETGIDLYLGSGGA
                     PEGVLAASALKCVGGHFQGRLIFRNDDERERARKTGVTDFDRKYDLHELVSKDAIFAA
                     TGVTKGAMLDGVRVADGFVSTHTLVMDSSTRTVQRIRTTRPL"
     gene            complement(2144..>2405)
                     /locus_tag="VF686_01655"
     CDS             complement(2144..>2405)
                     /locus_tag="VF686_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660541.1"
                     /translation="LPAPVAIAAQVNTLRVTNSRLAGEVARIDWQTTGDPAAYAAEVQ
                     ARVNAVPVAPVTAETQAEMDAFIARTRERGRAPPPVDRRPVQ"
BASE COUNT          342 a          873 c          819 g          371 t
ORIGIN      
        1 atcaccacca cggcggaacc gtcagcgtgg gcgccggtgg cctcgaccat gcgcgtggcc
       61 tgatcggtga cctgtttctc cacctcccgc cgggcgacgt tcagcgcgtc cagctcctgc
      121 gccagagcgg cggcctcgac cggatcgtcg gtcgacagca ggcgcgcgcc cagatccgac
      181 ttgccgatcc gtccgccggc attgatccgc gggcccagaa tgaagcccgc atggttcgac
      241 ttggccggcc ccggctcggc ccccgccgcc gccagcagcg cccgcagccc cggattggcc
      301 cattccgaca tccgccgcag gcccagactg gtcagggccc ggttgaaccc ggtcaggccc
      361 gtgacgtcgc agatcgcgcc catggccgcc agatccagcc actggcgaat atccggctgc
      421 tcgcgtccct cgaacagtcc gcgtccccgc gcctcgcggt tcagcgccgc cagcagcacg
      481 aacaccacgc ccgccgccgc cagattgccc tgccccgagt tgcagcccgg ccggttcgga
      541 ttgactacgg cggcacagac cggcggatgc tcgcgcatca tgtgatgatc tatgaccacg
      601 accttcagcc cgatctcggc ggcatgggcc actgcctcgt tggccgccgc gccgcagtcc
      661 acggtgatga ccagatcggc gccggaggcc ttcagcgtgt cgaacgcctt ggcgctgggg
      721 ccgtatccct cggtcatccg gtccggcaca tagatcggca gatccgcgcc catggcccgg
      781 aaccagcgca ccagcagggc cgcgctcgag gcgccgtcga catcgtagtc ggcgaagacg
      841 tggatcgagg cctgcgcctg aagcgcgtcg aggatcgccc cggccgccgc gtccatgtcc
      901 atgaagctgg agggatcggg gaacagcgcc ttcagcgtcg gctgcaggaa gtccgcgccc
      961 tgatccgccc gcacgccccg cgccgccagc gcccgcgcca gcggctcttc cagaccgagg
     1021 gtctgcatat gcgaccggat cagccccgcc tcggccggcc tctgtcgcca ggcgcggccg
     1081 gacagggagc gggacacgcc cagaaaggct ggcgcgccgg tcccgtcagc catcagagag
     1141 gccgcgtcgt gcgaatgcgc tgaaccgtgc gggtcgagga atccatcacc agcgtgtggg
     1201 tcgagacgaa gccgtcggcg acccggacgc cgtccagcat ggcgcccttt gtcacgcccg
     1261 tggcggcgaa gatggcgtcc ttggagacca gctcgtgcag gtcgtatttg cggtcgaagt
     1321 cggtgacgcc cgtcttgcgc gcccgttccc gctcgtcgtc gttgcggaag atcagccggc
     1381 cctggaaatg gccgccgacg cacttcagcg cactggccgc cagaacacct tccggcgccc
     1441 cgcccgatcc gagatacaga tcgatgcccg tctcgggttc ggccgtgtgg atcacgcccg
     1501 ccacgtcgcc gtcggtgatc agcagcaccc gggccccgac ctcgcgcaag gcggcgatca
     1561 gatcggcgtg gcgcggccgg tccatgacgc agacggtgat gtcctccggc ttcacgccct
     1621 tggccgaggc cagcgagcgg acattgtcct gcggcttcat gtccagatcg acggtcccag
     1681 gcgcaaaccc cggcccgatg gcgatcttgt ccatataggt gtcgggcgcg tgcagcatgc
     1741 cgccgcccgg cgacatcgac agcacgcaca gggcgttggc catggccttg gccgtcaggg
     1801 tggtgccttc cagcgggtcg agggcgatgt cgatcgccgg ccctttgccg gtgccgacct
     1861 tctcgccgat gtacagcatc ggcgcctcgt cgcgctcgcc ctcgccgatg acgatcttgc
     1921 cgtcgatgtc gaggttgttc agcgccgtgc gcatggcgtc cacggccagc tggtcggcct
     1981 gtttctcgtc gccgcggccg atctgggcgt agctggcgat ggcggccagc tcggtcaccc
     2041 gcgcggcgtc ggcggcgagc gtggatgcgt agggagccga agcggccatg acgtttctcc
     2101 tgagagagga tgattcccgg attctgccgt ccgggcgact gttctattgg accggacgac
     2161 gatccacggg cggcggggcg cggccgcgct cgcgggtgcg ggcgatgaag gcgtccatct
     2221 cggcctgcgt ctcggccgtc accggcgcga cgggaaccgc attgacgcgg gcctgaacct
     2281 cggcggcgta ggcggcgggg tcgcccgtgg tctgccagtc gatgcgcgcg acctcgccgg
     2341 ccagtctgga attggtcacc cgcagcgtat tgacctgggc cgcgatggcg accggcgccg
     2401 gcagc
//
LOCUS       DASVVN010000106         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_451393,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000106 DASVVN010000000
VERSION     DASVVN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451393"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..980
                     /locus_tag="VF686_01660"
     CDS             201..980
                     /locus_tag="VF686_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8660542.1"
                     /translation="MSRLWAFLLSMDSRAWRAVAVTVGLFASIGLIFLFGRTTLGVET
                     EQGLERWLSGLNGSGWGLPATVLLFTLAAFVGAPQFLLIAACVVAFGPWFGFLYAWVA
                     TVVSAAVTFYVGRLVGARSLQRFGGARLNRLSRYVGKNAFAGSFIIRNVPSAPFIVVN
                     MAFGVSQARFSSFISGCALGVLPKTALVAFFGGSFMTAVAGDGVWTSAILAGVGLGWL
                     ALMLAARGFMQRRATALEASGEGVRPAGEGPSGAGSAQAGG"
     gene            1141..>2014
                     /locus_tag="VF686_01665"
     CDS             1141..>2014
                     /locus_tag="VF686_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8660543.1"
                     /translation="MLSSVFGLISNDIAIDLGTANTLIYMKGKGIVLNEPSVVALRNL
                     GGRKVVHAVGLEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKHFIRKVHNRKGF
                     VNPKVIVCVPSGATAVERRAINDSCLNASARRVGLIDEPMAAAIGAGLPIHEPTGSMV
                     VDIGGGTTEVAVLSLSGIVYSRSVRVGGDMMDDAIVAYMRRHHNLLIGETTAERIKKE
                     IGTARAPVDGEGLSIEVKGRDLMQGVPREVRISEAQAAQALAEPVSQIVEAVKVALEA
                     TPPELAADIADKGIM"
BASE COUNT          270 a          703 c          707 g          334 t
ORIGIN      
        1 aggtcttccc catcgccgac gccctgtcgg agcgcggcgt ccccttcgtg ttcgccaccg
       61 gctacggccg cgccggcctg cgcgaccagg acaccgcccg gccggtggtg cagaagccgt
      121 acagcaccca ggacctcgcc cggacgctcc gggcggcggg ggtgctcgac taagctcgcg
      181 ggcgcggtac acccggcgcc atgtcccgac tctgggcctt cctgctgagc atggactccc
      241 gcgcctggcg cgcggtcgcg gtcaccgtcg ggctgttcgc ctccatcggc ctcatcttcc
      301 tgttcgggcg gaccacgctc ggggtggaga ccgagcaggg gctggagcgc tggctcagcg
      361 gtctgaacgg cagcggctgg ggcctgccgg cgacggtcct gctgttcacg ctcgccgcct
      421 tcgtcggcgc gccgcagttc ctgctgatcg ccgcctgcgt cgtcgcattc gggccgtggt
      481 tcggcttcct ctacgcctgg gtggcgacgg tggtgtccgc ggcggtcacc ttctacgtcg
      541 ggcgactggt cggcgcgcgc tcgctgcaac gcttcggcgg cgcgcgcctg aaccgactgt
      601 cgcgctatgt cggcaagaac gcattcgccg gctcgttcat catccgcaac gtgccgtcgg
      661 cgccgttcat cgtggtcaac atggccttcg gcgtctcgca ggcgcggttc tcgagcttca
      721 tctcgggctg cgccctcggc gtgctgccga agactgcgct ggtcgccttc ttcggcggct
      781 cgttcatgac cgccgtggcc ggcgacggcg tctggacctc ggccattctg gccggcgtcg
      841 gcctgggctg gctggcgctg atgctcgccg cgcgcggctt catgcagcgg cgcgcgacgg
      901 cgctggaggc ctccggggag ggggttcgcc ccgcgggtga ggggccttcc ggggccggat
      961 cggcccaggc gggcggttag tccaccgaat tcgttgcaaa ttcacccggg gacgtgcaca
     1021 gggctggcct aattccgccg gcttgggctt agactgtcct cgcgaagaga ttcgcgcggc
     1081 gggcgacgct tggagtcggg acttctcgtt tgaacccgcc cgccgtttgg gaaacgctcg
     1141 atgctgtctt ccgtctttgg cctgatctcg aacgacatcg cgatcgatct cggcacggcc
     1201 aacacgctga tctacatgaa gggcaagggc atcgtgctga acgagccctc cgtggtcgcc
     1261 ctgcgcaatc taggcggccg caaggtcgtc cacgcggtcg ggctcgaggc caagcagatg
     1321 ctgggccgca cccccgggca catggaggcc atccgcccca tgcgcgacgg ggtcatcgcc
     1381 gacttcgaag tcgccgagga gatgatcaag cacttcatcc gcaaggttca caaccgcaag
     1441 ggcttcgtga atccgaaggt gatcgtgtgc gtcccctcgg gcgccaccgc cgtggagcgc
     1501 cgcgccatca acgacagctg cctgaacgcc tcggcacgcc gggtcgggct gatcgacgag
     1561 cccatggccg cggccatcgg cgccggtctg ccgatccacg agccgaccgg ctcgatggtg
     1621 gtcgacatcg gcggcgggac caccgaggtg gcggtgctgt cgctctccgg catcgtctac
     1681 tcgcgctcgg tgcgggtcgg cggcgacatg atggacgacg ccatcgtggc ctacatgcgc
     1741 cggcaccata acctgctgat cggcgagacc acggccgagc ggatcaagaa ggagatcggc
     1801 accgcccggg ctccggtcga cggcgagggc ctctccatag aggtcaaggg ccgcgacctg
     1861 atgcagggcg tgccccgcga ggtgcgcatc agcgaggccc aggccgccca ggcgctggcc
     1921 gagccggtca gccagatcgt cgaggcggtc aaggtggcgc tggaagccac cccgcccgag
     1981 ctggcggccg acatcgccga caagggcatc atgc
//
LOCUS       DASVVN010000107         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_372326,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000107 DASVVN010000000
VERSION     DASVVN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372326"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF686_01670"
     CDS             <1..327
                     /locus_tag="VF686_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX8660544.1"
                     /translation="FLESLGLAEPGLNRLIREAYALLGLQSYFTVGPKEARAWTIPIG
                     ATAPQAAGVIHTDFEKGFIRAETIAYDDFVALKGEAKAREAGKLRAEGKAYVVKDGDV
                     MNFLFN"
     gene            complement(320..805)
                     /locus_tag="VF686_01675"
     CDS             complement(320..805)
                     /locus_tag="VF686_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660545.1"
                     /translation="MTLHRLAPIALFALAACGPSETNPPTEPAAPISTPEAEEPAPPT
                     PPPGVGAIMPGSGPQTFVGRWAANRAWCANPQGAERPIEITTTRFEGYENSCGILSVS
                     QVRDGYEVGLACSAEGQTSRERVRLNVQGDVMRLTWLNRNSAVVSLARCPAPGEAKPA
                     S"
     gene            936..1199
                     /locus_tag="VF686_01680"
     CDS             936..1199
                     /locus_tag="VF686_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660546.1"
                     /translation="MTQNTNPWAAQVDPLAQDIAAVLKRMGGSAHQRDVVLCVAAMKR
                     QRGETVAQDLANKIVEVFEKYRDLFFKPFGEGSMRWALQPGVA"
     gene            complement(1277..1807)
                     /locus_tag="VF686_01685"
     CDS             complement(1277..1807)
                     /locus_tag="VF686_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX8660547.1"
                     /translation="MRNILLATAALTLLAAPAMAQSISSPQVKGSIGYTQLDGDDASL
                     GAITGRMTANFNRYVGVEGEASFGVKDDEITVAGVTGDLSHEYDAAAYVVGTLPISPN
                     FELFGRVGYGTTSIKAEAAGLSTTEDGESVNYGAGANYFFDGRNGVRADWTRRDFTDD
                     NGGELDTYGLSYVRRF"
     gene            complement(1969..>2771)
                     /locus_tag="VF686_01690"
     CDS             complement(1969..>2771)
                     /locus_tag="VF686_01690"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX8660548.1"
                     /translation="GSVTLCARTPLDRLVVEAPDGRLIEASPRVVADPAEITEPVDWV
                     LVATKAYDVAAASRWLAPLLGPDTRVAVLQNGVEHVERFAPFVAVERIVPVVVDIPAE
                     RTAPGRIQHGRDGTIVVPAGPVGEAFAALFADSVIDASTTDDFVTVAWTKLSLNCAGA
                     VNALTGLPGGVVRQPTVAELMRGMVRECVMVGRAVGANLGDHLPDWVVERAETSPPDS
                     VNSLLGDRLAGRPMEWDARNGVIARLGAVHGIPTPLNAMAATLLSVTK"
BASE COUNT          442 a          920 c          955 g          454 t
ORIGIN      
        1 ttcctcgaaa gtctggggct ggccgagccg ggcctgaatc gcctgatccg cgaggcctac
       61 gccctgctgg gcctgcaatc ctatttcacc gtaggaccga aagaggcgcg cgcctggacc
      121 atcccgatcg gcgccaccgc gcctcaggcc gccggcgtga tccacaccga tttcgaaaag
      181 ggcttcatcc gcgccgaaac catcgcctat gacgacttcg tggccctgaa gggcgaggcg
      241 aaggcgcgcg aggccggcaa gctgcgcgcc gagggcaagg cctatgtcgt caaggacggc
      301 gacgtgatga acttcctgtt caactagccg gcttcgcctc gcccggcgcc gggcaacggg
      361 cgaggctgac cacggcgctg ttccggttca gccatgtcag ccgcatgacg tcgccctgca
      421 cgttcaggcg cacccgttcg cgcgaggtct ggccctccgc cgagcaggcc agcccgactt
      481 catagccgtc ccggacctgg ctgaccgaca ggatgccgca actgttctca tagccctcga
      541 accgggtggt ggtgatctcg atcggccgtt ccgcgccctg cggattggcg caccatgcgc
      601 gatttgcagc ccaccggccg acaaaggtct gggggcctga tcccggcatg atcgcgccga
      661 cgcccggcgg cggtgtcgga ggcgctggct cttcggcctc gggcgtgctg atcggggcgg
      721 ccggctccgt cggcggattg gtctcggacg gcccgcaggc ggcgagggcg aacagggcga
      781 tcggagcgag gcgatgcagg gtcatcgctc tggaacgcgg ttcgcgggcg acggctcccg
      841 atgttcgccc cgatcacgaa ttcgcggcca cgcttgcatc cgccggaaga ttttgcgacc
      901 ggttcgccag tgtcgagaac aagggggcgt cccgcatgac tcagaacacc aatccatggg
      961 cggctcaggt cgacccgctg gcgcaggaca tcgccgcggt gctcaagcgc atgggcggct
     1021 cggcccacca gcgcgacgtg gtcctgtgcg tggccgcgat gaagcgacag cggggcgaga
     1081 ccgtggccca ggacttggcc aacaagatcg ttgaggtgtt cgagaaatac cgcgacctgt
     1141 tcttcaagcc gttcggcgaa ggctcgatgc gctgggccct gcagcccggc gtcgcctgat
     1201 ccatcaccat tgaaccaacg aaaaggcgcg tctcctcagg gaggcgcgcc ttcgtcgttt
     1261 cgggagccgt cagcctttag aagcggcgga cgtagctcag gccgtaggtg tccagttcgc
     1321 cgccgttgtc gtcggtgaag tcgcggcggg tccagtcggc gcggacgccg ttgcggccgt
     1381 cgaagaagta gttggcgccg gcgccgtagt tgacgctttc gccgtcttcg gtggtggaaa
     1441 gaccggcggc ctcggccttg atcgaggtgg tgccgtagcc gacgcggccg aacagttcga
     1501 aattcgggct gatcggcagg gtgccgacga cataggccgc tgcgtcgtat tcgtgggaca
     1561 ggtcgccggt gacgccggcg acggtgatct cgtcatcctt gacgccgaac gaggcttcac
     1621 cctcaacgcc gacgtagcgg ttgaagttcg cggtcatgcg gccggtgatg gcgcccagcg
     1681 aggcgtcgtc gccgtccagt tgggtgtagc cgatggagcc cttgacctgc gggctggaga
     1741 tgctctgggc catggccggc gccgccagaa gagtaagagc agcggtggcg agaagtatgt
     1801 tacgcattgt ttcttgtatc ctctagatgt ggcgacctgt ggtcaccagc cccatccagc
     1861 ggggacacaa atgggtcggc gcgaactcag ttccagactt gggcggagac ttcattcgaa
     1921 ctgtgcatac cgaagcacat acggcgttat acttgccgcc ttgccgcctt atttcgtcac
     1981 cgacagcagg gtcgccgcca ttgcgttcag gggcgtcggg attccgtgca ctgcgccaag
     2041 ccgggcaatg acgccgttgc gggcgtccca ctccatcggc cgccccgcca gccggtcgcc
     2101 gagcagggag ttgaccgaat ccggcgggga ggtttcggcg cgctccacga cccagtccgg
     2161 gagatgatcg cccagattcg cgccgacggc cctgccgacc atgacacatt cgcgcaccat
     2221 gccccgcatc agttcggcga ctgtgggctg tctcaccacc ccgcccggca ggcccgtcag
     2281 ggcgttgacc gccccggcgc agttcagcga cagcttggtc caggcgacgg tgacgaagtc
     2341 gtcggttgtc gaggcgtcga tgacgctgtc ggcgaacagg gcggcgaagg cctcgcccac
     2401 cggtcccgcc gggacgacaa tcgtgccgtc gcgcccgtgc tggatgcggc cgggcgcggt
     2461 gcgttcggcg gggatatcga cgaccaccgg cacgatgcgc tcgaccgcga cgaagggggc
     2521 gaaccgctcg acatgttcga cgccgttctg cagcacggcg acccgggtgt ccggccccag
     2581 cagcggcgcc agccagcgcg aggcggcggc gacgtcatag gccttggtcg ccacgagcac
     2641 ccagtcgacc ggttcggtga tttcggcggg atccgcgacc acccgcggac tggcctcgat
     2701 cagccggccg tccggcgcct cgaccaccag ccggtccagc ggcgtccgcg cgcaaagggt
     2761 caccgacccg a
//
LOCUS       DASVVN010000108         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_451420,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000108 DASVVN010000000
VERSION     DASVVN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451420"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF686_01695"
     CDS             <1..789
                     /locus_tag="VF686_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660549.1"
                     /translation="PFARHARRGRCGAAGPNTATLAPSYTRMAGGLRIERPIRVRPAQ
                     YERWRKLAIVAVSLGLHTLVLGYVALKSFTPPRQYIDGTHGDPLFPAPTIYLQVEPRP
                     LLRGETARTRETPPPEAIVQAIPDAGTRLTEITGATGSADVGDRRLQPMPRGAADTPV
                     LPADAETLSWRVRPAAPGDGIGRGLRASRLGCTAPDLLSASERDICDERFARRASLAK
                     SLGPPPDRDRDEAFARQADANEAWRAYTRDEGPYPGLRSLFTTR"
     gene            846..>1554
                     /locus_tag="VF686_01700"
     CDS             846..>1554
                     /locus_tag="VF686_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660550.1"
                     /translation="MRKPELSWPTGADRWRKPAIVAASVLLHAGLLAWIGLRTMGLDS
                     YAIRDPEPTIYLQLEPRPLLADETPRQPVAARQVEAPPLARAMTTPNLRDEDEDETPA
                     PPSPRLAAPAPGAPPAPADGAWAVRPRTLGDRVAQGLRTSPLGCAAGAVLSDAERAIC
                     DDRFGQRAATARPIEGTGNPERDARFAREGARRLQEYEARRRPLAGGSGNVGPQDGPG
                     SNFGIGVAGAHLDPSLRP"
BASE COUNT          216 a          633 c          515 g          190 t
ORIGIN      
        1 cccttcgccc gccatgcgag aagaggccgg tgcggggcag caggccccaa taccgccacg
       61 ctagccccct cctatacgcg tatggcgggt ggactgcgca ttgagcgacc gatccgggtt
      121 cggcccgcgc aatacgagcg ctggcgcaag ctggccattg tggcggtgtc gctcggcctg
      181 cacacccttg tgttgggcta tgtcgccctc aagagcttca cccctccgcg ccagtacatc
      241 gacggcacac atggggaccc gctgttcccc gcgccgacca tctatctgca ggtcgaaccc
      301 cgccccctgc tgcgcgggga gacggcccgg acccgcgaaa cgccgccgcc cgaagccatt
      361 gtgcaagcca tcccggatgc aggcacgcgc ctgacagaga tcaccggggc cacgggctcg
      421 gccgacgtcg gcgaccgtcg gttgcagccc atgcctcgcg gcgcagccga cacccccgtc
      481 ctccccgctg atgccgaaac gctgtcgtgg cgggtccggc ctgccgcgcc cggcgacggc
      541 atcggccggg gcctgcgcgc cagccgcctc ggctgcaccg cccccgatct gctcagcgcc
      601 agcgagcggg atatctgcga cgaacgattc gcccgacgcg cctccctcgc gaagtcgctg
      661 ggaccgccgc ccgaccggga tcgggacgag gctttcgccc gtcaggccga cgccaacgag
      721 gcttggcgcg cctatacgcg tgacgaaggt ccctacccgg gcctgcgctc cctgttcacc
      781 acgcgctagc gccgcaatgc cgccacgctc gccacttcca gtgaaggccg gacgcagggg
      841 ggcggatgcg aaaacctgag ctgagctggc cgaccggggc ggaccgctgg cgcaagcccg
      901 ccatcgtcgc ggcctccgtc ctgctgcacg cgggtctgct ggcgtggatc gggctgcgca
      961 ccatgggtct ggactcatac gcgatccggg atccggagcc gaccatctat ctccagctcg
     1021 aaccccgccc cctgctggcc gacgagacac cgcgccagcc tgtggccgcc cggcaggtcg
     1081 aggcgccgcc gctggcccgc gccatgacca cgccgaacct gcgcgacgag gacgaagacg
     1141 agacgccggc gcccccctcc ccccgcctcg cggctcccgc accgggcgca ccgccggccc
     1201 ccgcggacgg ggcatgggcc gtgcgccccc gcaccctcgg cgaccgggtg gcccagggcc
     1261 tgcggaccag ccccctcggc tgcgccgcgg gcgccgtgct cagcgacgcc gagcgcgcca
     1321 tctgcgacga ccgcttcggc cagcgggccg cgaccgcccg gcccatcgag ggcacaggga
     1381 atcccgaacg cgacgcccgc ttcgcccgcg aaggcgcccg ccgcctgcag gaatatgagg
     1441 ctcggcgccg tcccctcgcc ggcggcagcg gcaacgtcgg cccgcaggac ggcccgggct
     1501 ccaacttcgg catcggcgtc gcgggtgctc atctggaccc ctccctgcgc cccg
//
LOCUS       DASVVN010000109         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_293128,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000109 DASVVN010000000
VERSION     DASVVN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293128"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..2181
                     /locus_tag="VF686_01705"
     CDS             46..2181
                     /locus_tag="VF686_01705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8660551.1"
                     /translation="MKRLLVGAALGALLLPASIASAQTADGHDHGCIDDGCTIVELFQ
                     TPAEGATILGYQGTETPKYGTWGVDLNGRDTSVNPGDSFMRYANGAALDRLQIPSDRT
                     SYGSFALLRELSDNRVKAMIEGLTARTDLTAGSDEQKIADAYRSYMDEARIEALDARP
                     LAPYLAAIRAANTHELAGVYMGQTQARIGGSIFGTGITTDQKAPTRYVVSTGQSGLGL
                     PNRDYYLEQRWAEKKVLYQAYVARMLEMAGWESPTAAAEAIVAFETRIAEAHWTAAEN
                     RNRDETYNDYTIARLSEEAPGFPWQAYYTAVGVGEVPRLIVRQDTAMPKIAAIYAETP
                     IEVIQAWQTFHTVDEAAPRLSRRFADAQWEFRSRDLNGQPMQRPRDKRGISFAEGMLG
                     EAVGRQYVAEYFPAESKAKMEALVANLRVALGNRIQNYSWMSAETKAAAEDKLNKFTV
                     KIGYPNKWRDYSALEVRPDDLFGNAERAGLFQWNYQLNRLNDPVDKTEWGMTPQTVNA
                     YYNSTNNEIVFPAAILQPPFFDPDADPAVNYGGIGGVIGHEIGHGFDDQGSKSDGDGV
                     LRSWWTTEDRANFDGLTKRLGEQYAQFEPIPGFTINPGLTMGENIGDAAGNAVGLEAY
                     HLSLNGQPAPVLDGTTGDQRFFYGWAQVWQSKMRDDALRNQIATDSHSPAEFRVIGPV
                     RNIDAWYEAFDVQPGSKYYLAPEDRVRIW"
BASE COUNT          450 a          859 c          761 g          348 t
ORIGIN      
        1 tcgcgcccga cgctgtccgg aacggaagcg aagtcgagga aatccatgaa acgtctgctg
       61 gtcggcgccg ctctcggcgc cctgctgctg cccgcctcca tcgcatccgc ccaaacggcc
      121 gacggtcacg atcacggctg catcgacgac ggatgcacca tcgtcgaact gttccagacg
      181 ccggcggaag gcgccacgat cctcggctat caggggaccg aaactccgaa gtacggaacc
      241 tggggcgtcg acctgaacgg gcgcgacacc tcggtcaacc caggggacag cttcatgcgc
      301 tacgccaacg gcgccgcgct ggaccggctg cagatcccct cggaccgcac cagctacggc
      361 tcgttcgccc tgctgcgcga actgtccgac aaccgcgtca aggcgatgat cgaaggcctg
      421 acggcccgca ccgacctgac cgccggctcg gacgaacaga agatcgccga cgcctatcgc
      481 tcctacatgg acgaggcccg catcgaggcg ctggacgccc ggccgctggc cccctacctg
      541 gccgccatcc gcgccgccaa cacccatgag ctggccggcg tctacatggg ccagacccag
      601 gcccgcatcg gaggctccat cttcggcacc ggcatcacca ccgaccagaa ggcgccgacc
      661 cgctacgtcg tctcgaccgg ccagtcgggc ctcggcctgc ccaaccgcga ctattatctg
      721 gagcagcgct gggccgagaa gaaggtcctg tatcaggcct atgtcgcccg catgctcgag
      781 atggccggct gggaaagccc gacggcggcg gcggaggcca tcgtggcctt cgagacccgc
      841 atcgcagagg cgcactggac cgcggccgag aaccggaacc gcgacgagac ctacaacgac
      901 tacaccatcg cccgtctgtc cgaggaggct ccgggcttcc cgtggcaggc ctattacact
      961 gccgtcggcg tcggcgaggt tccgcgcctg atcgtccgcc aagacacggc catgccgaag
     1021 atcgccgcca tctacgccga gaccccgatc gaggtcatcc aggcctggca gaccttccac
     1081 accgtggacg aggctgcgcc gcgcctgtcg cgccgcttcg ccgacgccca gtgggagttc
     1141 cgctcacgcg acctgaacgg ccagccgatg cagcgtcccc gcgacaagcg cggcatcagc
     1201 ttcgccgagg gcatgctggg cgaggccgtc ggccgccagt atgtggccga gtatttcccg
     1261 gccgagtcca aggccaagat ggaggccttg gtcgccaacc tccgcgtcgc cctgggcaac
     1321 cgcatccaga actacagctg gatgagcgcc gagaccaagg cggcggccga ggacaagctg
     1381 aacaagttca cggtcaagat cggctatccg aacaagtggc gtgactattc ggcgctggaa
     1441 gtccgccccg acgacctgtt cggcaacgcc gagcgggccg gcctcttcca gtggaactac
     1501 cagctgaacc gcctgaacga cccggtcgac aagaccgaat ggggcatgac gccccagacg
     1561 gtgaacgcct attacaactc gaccaacaac gagatcgtct tcccggccgc catcctgcag
     1621 ccgccgttct tcgatccgga cgcggatccg gccgtcaact acggcggcat cggcggcgtc
     1681 atcggccacg agatcggcca cggcttcgac gaccagggct ccaagtccga tggcgacggc
     1741 gtcctgcgca gctggtggac gacggaagac cgggccaatt tcgatggcct gaccaagcgg
     1801 ctgggcgaac aatacgccca gttcgagccg atcccgggct tcaccatcaa ccccggcctg
     1861 accatgggcg agaacatcgg cgacgccgcc ggcaatgcgg tgggtctcga ggcctatcac
     1921 ctgtcgctga acggccaacc tgccccggtg ctggacggca cgacgggcga ccagcgcttc
     1981 ttctacggct gggcccaggt ctggcagtcg aagatgcgcg acgacgccct gcgcaaccag
     2041 atcgccaccg actcgcacag cccggcggag ttccgcgtca tcggcccggt gcgcaacatc
     2101 gacgcctggt atgaggcgtt cgacgtccag ccgggttcga aatactacct cgccccggaa
     2161 gaccgggtgc ggatctggta gggccgcgag gcacgaactg aagtgggccg gagcggcgac
     2221 gctccggcct ttttttattc caactggctt atcctccacc gtgcaccccg gggaatgccg
     2281 gggtccagat ggcgagcgca gtgatccgaa cagggatcgc gtcgccaatc cctgtcgacc
     2341 cggtccgtga tctggatccc ggcattcgcc gggatgcacg gtgataatgc gagggttggg
     2401 gtgcgacctg cgctcaag
//
LOCUS       DASVVN010000110         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_424735,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000110 DASVVN010000000
VERSION     DASVVN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424735"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2784
                     /locus_tag="VF686_01710"
     CDS             <1..2784
                     /locus_tag="VF686_01710"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8660552.1"
                     /translation="WAQTPETPTDPWAQAGSDLPADASVRFGVLPNGMQYAIMRNATP
                     PERASLRLRIDAGSLMENEHQLGLAHFMELMAFNGTTNVPQNDLLSILERLGLAFGAD
                     TNAATGFDQTFYILEVPRTNDETIDTALHIMREQVSEALMDPVAIDEERGVIEGEERL
                     RNTPSLRASRAQLDLLAPGQRISQRMPIGDLEIIRTAPRERFVEFYNSFYRPSRATFI
                     AVGDFDVDVMERKIRAAFADWTSRGAPGAEPDLGVAAPRAPETRVMVEAGLSSSIAIS
                     WTRPPDLEPDTVARRRKDLVQNLGLAVLNRRFAEMSRTDNPPFVTASSATDDLARSVQ
                     VATVSAAFVPGQWRRALETIDQEQRRLVEYGVSEAELQREITSWRTVLENAVAGAATR
                     QTSGLSNGLLSAVNDKQVFTTPQTNLDLFNAAVDGLSIAEMNAAIKPVFEGGGPLIMV
                     TSPVALEGGEAAVTAALETSRGVAVAALGTPEAKAWDYTNFGAPGVVKSRRELAELGA
                     TEVTFENGVVLVVKPTTFADESISVQIVTGIGDQAFSPTEFDPRQAAINTLRSGGLGR
                     LNVDEISRALNGRVVGGGLGVAENRFTLGGATRPEDLELEMQFLAAHLTDPAFRSAPY
                     EQMKALYPASLELTRATPGGAFGMEAGALLAGGDRRKAVVPAELVASWAIEPIRDSLK
                     DLLEDGPLHITMVGDVTLEDAIRTTGATFGALPPRPPRAAGAPGADVRRFPAPTPQPL
                     RFTHTGPAEQALGFIAWPTIDAKGDRTRARRIQMLTEVLKLRVVEEIRERQALAYSPG
                     VSATFSEVYDDYGYVSITAQTAADKLPAFFAAVDDIIAKLTTGQISEDELNRARAPMI
                     ASARRAMNSNGWWLLNLADVQENPQNINQVLDGVADMEAVTPEDLQTLAREYLRPETA
                     WKATVTAQATPAA"
     gene            complement(2877..>3323)
                     /locus_tag="VF686_01715"
     CDS             complement(2877..>3323)
                     /locus_tag="VF686_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660553.1"
                     /translation="IRDRSGPRGLVMIGRAANDTGFTPLDQRRILAMRPWLLHALDAT
                     PTAEFSTVEAGDEAILLCDHQGLVVQMGPNAAKLLLYAGNGRIAQREVAVGIGLGRPA
                     ERVQAELGLTAGTFGRHLEDIHERLGVRSRSELMTTLMGHRTAGSG"
BASE COUNT          550 a         1180 c         1098 g          495 t
ORIGIN      
        1 tgggcgcaga ccccggaaac cccgaccgat ccgtgggcgc aggcgggcag cgatctgccg
       61 gccgacgcca gcgtgcgctt cggcgtcctc cccaacggca tgcagtacgc gatcatgcgc
      121 aacgccacgc cgccggaacg cgcctcctta cgcctgcgca tcgacgcggg ctctctgatg
      181 gagaacgagc accagctcgg cctcgcccac ttcatggagc ttatggcgtt caacggcacc
      241 accaacgtgc cccagaacga cctgctgtcg atcctcgagc ggttggggtt ggccttcggg
      301 gcggacacca acgccgcgac gggcttcgac cagaccttct acattctgga ggtcccacgc
      361 accaacgacg agaccatcga cacggcgctc cacatcatgc gcgagcaggt ttccgaggcc
      421 ctgatggacc ctgtggcgat cgacgaagag agaggcgtga tcgagggcga ggagcggctt
      481 cgcaacactc cgagcctgcg agcctcccgc gcccagcttg atctgctggc gcccgggcag
      541 cgcatctcgc agcggatgcc catcggcgac ctcgagatca tacgcacggc gccgcgggag
      601 cggttcgtcg aattctacaa cagcttctac aggccctccc gcgccacatt catcgcagtg
      661 ggcgacttcg acgtcgatgt gatggagcga aagatccggg cggcgttcgc cgattggacg
      721 tcgcgcgggg ctccgggcgc cgaacccgat ctcggtgtcg cggcgcctcg cgcgccggag
      781 accagggtga tggtcgaggc gggactgagc tcatcgatcg ccatatcgtg gacccggccg
      841 ccggaccttg agccggacac cgtcgcccgg cgccgcaagg accttgtcca gaacctgggg
      901 ctggccgtcc tgaaccgtcg cttcgccgaa atgtcgcgga cggacaatcc gccgttcgtc
      961 accgccagct ccgccaccga cgatctcgcg cggagcgttc aggtcgcgac ggtgtcggcc
     1021 gccttcgtgc ccggccaatg gcgccgggct ctcgagacca tcgaccagga acagcgtcgt
     1081 ctggtggagt atggcgtctc ggaggccgaa cttcagcgcg agatcacctc ctggcgtacc
     1141 gtgctcgaaa acgccgtggc cggggccgcg acgcggcaga cgtcgggtct ctcgaacggt
     1201 ctgctttcgg ccgtcaacga caagcaggtc ttcaccacgc cgcagacaaa cctcgacctg
     1261 ttcaacgcgg ccgtcgacgg cctgtcgatc gccgagatga acgccgcgat caagccggtg
     1321 ttcgagggcg gaggcccctt gatcatggtg acatcgccag tcgcgctcga ggggggcgag
     1381 gcggctgtga cggccgcgct cgaaacctct cgcggtgtcg ccgtcgcggc ccttgggacg
     1441 cccgaggcga aagcctggga ctacacgaat ttcggcgcgc cgggcgtcgt gaagagccgc
     1501 cgcgaattgg ccgagctggg agccacggag gtgacgttcg agaacggcgt cgtcctcgtg
     1561 gtgaagccga ccaccttcgc cgatgagtcg atctcggtgc agatcgtcac cggcatcggc
     1621 gaccaagcct tctcacccac cgaattcgat cctcgtcagg cggcgatcaa cacccttcgg
     1681 tccggcgggc tcggtcgtct caacgtcgac gagatcagcc gcgcgctgaa cggccgcgtc
     1741 gtcgggggcg gtctgggcgt ggcagagaac cgcttcaccc tcggcggcgc aacccggccc
     1801 gaggatctcg agctcgagat gcagtttctc gccgcgcacc tgacggatcc ggcgttccgg
     1861 tcggcccctt atgaacagat gaaggcgctg tatcccgcgt cgctggaact gacgcgggcg
     1921 acgcccggcg gggccttcgg catggaggcc ggggcccttc tggccggcgg cgacaggcgc
     1981 aaggcggtgg ttcctgcgga actggtggcg agctgggcca tcgaacccat ccgcgacagt
     2041 ctgaaagacc tgcttgagga tggcccgctg cacatcacca tggtgggcga cgtgacgctc
     2101 gaggacgcca tccgcaccac gggcgccacg ttcggagccc tgcctccccg tcccccgcgc
     2161 gcggccggag ccccgggagc cgacgtccgg cgtttcccgg cgccgacgcc ccaaccgctc
     2221 cggttcaccc acaccggccc ggccgagcag gcgctcggct tcatcgcctg gccgacgatc
     2281 gacgccaagg gcgaccgcac ccgggcgcgc cgcatccaga tgctcaccga ggtcctcaag
     2341 ctgcgtgtgg tcgaggagat tcgtgagcgt caggcgctgg cctattcgcc gggcgtcagc
     2401 gcgaccttct cggaggtcta tgacgactac ggctatgtct cgatcacggc ccagaccgcg
     2461 gcggacaagc tcccggcctt cttcgcggcc gtggacgaca tcatcgccaa actcacgacc
     2521 ggccagatct cggaggatga gctgaaccgc gcccgcgcgc cgatgatcgc ctcggcccgg
     2581 cgcgccatga acagcaacgg ctggtggctt ctgaacctcg cggatgtcca ggagaacccg
     2641 cagaacatca accaggttct ggacggcgtc gccgacatgg aagctgtgac gccggaggat
     2701 ctgcagaccc tcgcccgcga atatctgcgg cccgaaacgg cctggaaggc caccgtcacc
     2761 gcccaggcga cccccgcggc ctgagcggcg ctcgccggcc ccggcgcaag cgccgtcggg
     2821 gccggcgaca ccaaggtggc cccttgtgcg ccggagagca ccggcgacca cggcgcctat
     2881 ccagaccccg ccgtgcgatg gcccatcaat gtcgtcatca gttccgaccg tgaacggaca
     2941 ccgagccgtt catggatatc ctccaggtga cggccaaagg tgccggcggt caggcccagc
     3001 tccgcctgca cccgctccgc cgggcgaccc aggccgatcc cgactgcgac ctcgcgctgt
     3061 gcgatcctgc cgttgccggc gtacaggagc agctttgccg cattgggccc catctgcacg
     3121 accagacctt gatggtcgca cagaagaatg gcctcgtcgc ccgcctcgac ggtgctgaac
     3181 tccgccgtcg gcgtggcgtc gagcgcatgc agaagccagg ggcgcatcgc caagatgcgg
     3241 cgctggtcca gcggcgtaaa tcccgtgtcg ttggcggcgc gcccgatcat gaccaggcca
     3301 cgcggaccgc tgcggtcgcg gat
//
LOCUS       DASVVN010000111         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_162394,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000111 DASVVN010000000
VERSION     DASVVN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162394"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF686_01720"
     CDS             <1..530
                     /locus_tag="VF686_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660554.1"
                     /translation="RSNLLLAKLATVAALALAAMALMLVASVAENLIKAGVFGRQLTF
                     SMTGEMAGQFLAFTGLSWLRILQFTMMGMLAAVVTRSLLAALFIPLVVGVAQFFSPNM
                     LMPMGVMPDAWLAVLLNPGAAVDIVKAMAEGGASAAALPDGVGVKAWLSLALWTFGPL
                     AAALAWFPRQDLSKE"
     gene            534..1427
                     /locus_tag="VF686_01725"
     CDS             534..1427
                     /locus_tag="VF686_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660555.1"
                     /translation="MLPIHAAIALALTGFAPQDPPQVPAAEPTQVDDVLVTGRPLREM
                     VENFVDAVVEPPRGRGPARWDRKVCVGVVNLRREPAQIIIDRVSQVALDVGLEIGEPG
                     CSPNILVLATNDGAGLATALIESRPNAFRPNFAGSSRSGSALERFRTTDSPVRWWHVA
                     LPVDSDTGAAAVRMPGEGAPKARTLGGLLRTEVRNDLRRAFVIVDMDEAAGVNFQQLS
                     DYVAMVSFAQIDPDADLTAYQTVLNLFEAPASVGGMTEWDRSYLTALYDAELNQRNPN
                     SQIGAVGGVMIQDREREPAQE"
     gene            complement(1431..1901)
                     /locus_tag="VF686_01730"
     CDS             complement(1431..1901)
                     /locus_tag="VF686_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660556.1"
                     /translation="MTTAEVSIENYRPDHAAAWRSLNEDWLVAGGYVLEPKDQLVLGD
                     PQGAILDRGGFIFIAEQAGRPVGCCSLMAMADGGFEVGKMMVLSGLRGQNLGWRLLDA
                     CEAAARTAGAPRLYLETNKAQTHAIALYRRFGFVDLPPQPSPYARCDVWMEKRF"
     gene            complement(1927..2319)
                     /locus_tag="VF686_01735"
     CDS             complement(1927..2319)
                     /locus_tag="VF686_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HEX8660557.1"
                     /translation="MDDEIRILGGRPVLLCADQGPQISSEADALDLIGGLWGREVDWL
                     ALPVARLAPDFLRLRTGLAGAVIQKFVTYSVRLAIVGDLSAAIAESDALRDFVRESNA
                     GRHVWFVPDLAALEDRLMEPAEAFSSRP"
     gene            complement(2400..>2742)
                     /gene="rplQ"
                     /locus_tag="VF686_01740"
     CDS             complement(2400..>2742)
                     /gene="rplQ"
                     /locus_tag="VF686_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX8660558.1"
                     /translation="SLIKHEQITTTLPKAKELRPFVEKLVTLAKKGDLHARRQAISQV
                     RDVPQVGKLFETIGPRYAERNGGYIRIMKAGYRHGDNAAMAVIEFVDRDADAKGLDSG
                     PVYAIEGDEEA"
BASE COUNT          430 a          932 c          921 g          459 t
ORIGIN      
        1 gccgctccaa cctgctgctc gccaagctgg ccacggtcgc cgcgctggcg ctggcggcga
       61 tggccctgat gctggtggcg agcgtggccg agaacctgat caaggcgggc gtgttcggcc
      121 gtcagctgac cttctcgatg acgggggaga tggcgggtca attcctggcc tttaccggcc
      181 tgtcgtggct gcgcatcctg cagttcacca tgatggggat gctggcggcg gtggtgacac
      241 gctcgctgct ggcggcgctg ttcattccgc tggtcgtcgg cgtggcgcaa ttcttctcgc
      301 ccaacatgct gatgccgatg ggagtcatgc cggacgcctg gctggccgtg ctgctcaacc
      361 cgggcgccgc ggtggatatc gtcaaggcga tggccgaggg cggcgccagc gcggcggccc
      421 tgcccgacgg cgtcggcgtc aaggcctggc tcagcctggc gctgtggacg ttcggtccgc
      481 tggcggcggc tctggcctgg ttcccgaggc aggatctctc caaggaatag accatgctcc
      541 ccatccacgc cgcgatcgct ctcgccctga ccggttttgc acctcaggat ccgccccagg
      601 ttcccgccgc cgaaccgacc caggtcgacg atgttctggt gaccggccgc cccttgcggg
      661 aaatggtcga gaacttcgtc gatgcggtgg tggagcctcc ccggggccgg ggtcccgccc
      721 gctgggaccg caaggtctgc gtcggcgtgg tcaatctgag acgcgagccg gcgcagatca
      781 tcatcgaccg ggtgtcgcag gtcgcgctcg acgtcggcct ggagatcggc gagcccggct
      841 gcagcccgaa catccttgtg ctggccacca acgacggcgc cggtctagcc accgcgttga
      901 tcgagtcgcg gccgaacgcc ttccggccca attttgcggg gtcatcgcga agcggctccg
      961 cgctggagcg tttccggacc acggacagcc ctgtccgctg gtggcacgtc gccctgcctg
     1021 tggactcaga cacgggcgcc gcagcggtgc gaatgccggg cgaaggggcg ccgaaagcca
     1081 gaacgctcgg aggcctgctg aggacggaag tccgcaacga cctgcgccgc gccttcgtca
     1141 tcgtcgacat ggacgaagcg gccggcgtga atttccagca gctcagcgac tatgtcgcca
     1201 tggtctcctt cgcccagatc gacccggatg cggatctcac cgcctatcag acggtgctga
     1261 atctcttcga ggcccccgcg tcggtcggcg gcatgacgga gtgggaccgg tcgtatctga
     1321 cggccttgta cgatgcggag ctgaatcaac ggaacccgaa ctcacagatc ggcgccgtgg
     1381 gcggcgtcat gatccaggac cgcgaacgtg agcccgcgca ggaatagcgg tcagaaccgc
     1441 ttttccatcc agacatcgca gcgcgcatag gggctgggtt gcggcggcag gtcgacgaag
     1501 ccgaagcggc ggtacagggc gatggcgtgg gtctgggcct tgttggtctc cagatacagg
     1561 cgcggcgcac cggccgtccg cgccgccgcc tcgcaggcgt cgagcagccg ccagccgagg
     1621 ttctggccgc gcaggccgga gaggaccatc atcttgccga cctcgaagcc gccgtcggcc
     1681 atggccatca gcgaacagca gccgaccggc cggcccgcct gttcggcgat gaagatgaag
     1741 ccgcccctgt cgaggatggc gccctgcggg tcgcccagca caagctgatc cttaggctcg
     1801 agcacatagc cgcccgccac cagccagtcc tcgttcagcg accgccaggc ggcggcgtga
     1861 tcgggacgat agttttcgat cgagacttcg gctgtcgtca tgcgccctcc tctcctcatc
     1921 tgggcgttac ggccgcgaag aaaaggcctc ggcgggctcc atcaaccgat cttccagcgc
     1981 cgcgaggtcg ggaacgaacc agacatggcg gcccgcgttg gattcgcgca cgaaatccct
     2041 gagcgcgtcg ctctcggcga tcgcggccga caggtcgccg acaatcgcca gccggacgct
     2101 gtaggtgacg aatttctgga tcaccgcccc cgccagaccg gtgcgcaggc gcagaaagtc
     2161 cggagccagc cgcgccacgg gcagcgccag ccagtcgacc tcccgccccc acaggccgcc
     2221 gatcagatcc agggcgtcag cctcgctgga tatctgcggc ccctgatcgg cgcatagcag
     2281 gaccggacgc ccgcccagaa tccgtatttc gtcgtccatg gaatccccgc gctaaaacga
     2341 aaaaaggcgg ccggttcgca ccggccgccc ttttctcaag cccctgacgg gaagcgtgct
     2401 tacgcttctt cgtcgccctc gatggcgtag accggacccg agtccaggcc cttggcgtcg
     2461 gcgtcgcggt cgacgaactc gatcacggcc atggcggcgt tgtcgccgtg gcgatagccg
     2521 gccttcatga tgcggatgta gccgccgttg cgctcggcgt agcgcgggcc gatcgtctcg
     2581 aacagcttgc cgacctgcgg aacgtcacgg acctggctga tggcctgacg acgcgcgtgc
     2641 aggtcgccct tcttggccag cgtgaccagc ttctcgacga agggacgcag ttccttcgcc
     2701 ttgggaagcg tggtcgtgat ctgctcgtgc ttgatcagcg ac
//
LOCUS       DASVVN010000112         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_214723,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000112 DASVVN010000000
VERSION     DASVVN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214723"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..469)
                     /locus_tag="VF686_01745"
     CDS             complement(140..469)
                     /locus_tag="VF686_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX8660559.1"
                     /translation="MLLSSDLWVSALIRRAELAGAFATVARKGDARAGTVIVKAFDTS
                     TRRARLYSEAFGPDGERLWMQPVESEFESELDAYVQRQAGYDPDIWVVEIEDREGRHF
                     ITEKVAV"
     gene            505..1386
                     /locus_tag="VF686_01750"
     CDS             505..1386
                     /locus_tag="VF686_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660560.1"
                     /translation="MARKLRLMTIGQTALKTKAALLAAAVLCAPQAASAGPADTYYER
                     AFMVAADRRCGLFDARIDAALGAATAQARGAALRSGTAEAELSATAARARSRAHAVSC
                     QDPQLATVKGRVDEAFSGWLRTPRMTFPGERANWTANRMRIAGAGWRLQQTSVVGASP
                     VAFGYAGEGQTSNLVAVVSFVGRSRPTGARIVLRDPNKAPRAWLAGDGLVPASSRASL
                     WAANVAPAERELLAEGRRAGEVWRFPASTAAALERLDPRETFAIEFHFRDGSVATAMF
                     EAGDFAAGRAFLAMGML"
     gene            complement(1390..>2179)
                     /locus_tag="VF686_01755"
     CDS             complement(1390..>2179)
                     /locus_tag="VF686_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8660561.1"
                     /translation="GLEVARTEAETREAGKTRFLANMSHELRTPLNAVLGFSDIMRQR
                     IFGPLPDRYGEYAENIHQAGGHLLDLINDLLDVAKIEAERYALTLERFDARETVSAAV
                     ALVRVNADDKGVSLSSVLPGDPVEVSADRRALKQITLNLLSNAVKFTPPGGTITVTVE
                     AIGPYLEVVVADTGVGIAPEDVRRLGRPFEQAGEIEQRRQGTGLGLSLVRAFAELHGG
                     RMSIDSTLGEGTAVTVRLPVALVARAPAPEGGAEIIPMPMKNGA"
BASE COUNT          309 a          764 c          754 g          352 t
ORIGIN      
        1 accatcgccg ggctcgtttt tcggcccgtt gaagatgacc atagcggggg cggcttgcgg
       61 ttccgttccc gaacgcggtt aagtatttcg caccgtcggc gacggcgtta acccgccggt
      121 ttccatgccg ggacccggat cagacggcga ccttctcggt gatgaagtgc cgcccctcgc
      181 ggtcctcgat ctcgacgacc cagatgtcgg ggtcgtagcc ggcctggcgc tggacgtagg
      241 cgtccagttc gctttcgaac tcgctttcga ccggctgcat ccacaaccgc tcgccgtcgg
      301 ggccgaaagc ctcactatag agtctggcgc ggcgggtgga ggtgtcgaac gcctttacaa
      361 tgaccgtgcc cgcgcgcgcg tcgcccttgc gcgcgaccgt cgcgaaggcc cccgccagct
      421 cggcgcggcg gatcagggcg gacacccaaa gatcgctgga tagaagcatt ttgataaccg
      481 tgcccggaaa ccgtaccgga acgcatggcg cgtaagttgc gcctcatgac gataggccag
      541 accgctctga agaccaaagc cgcgctgctc gccgccgccg ttctgtgcgc gccgcaagcc
      601 gcctcggccg gtccggccga cacctattac gagcgcgcct tcatggtcgc cgccgaccgg
      661 cgctgcggtc tgttcgacgc ccgcatcgac gccgctctcg gggccgcgac ggcccaggcc
      721 cggggcgcgg ccctgcgctc aggcaccgcc gaggccgagt tgagcgccac cgccgcccgc
      781 gcccgctcgc gcgcccacgc cgtatcctgt caggaccctc aactggcgac cgtgaagggc
      841 cgcgtcgacg aggccttctc gggctggctg cggacgccac gcatgacctt cccgggcgag
      901 cgcgccaact ggacggccaa ccgcatgcgc atcgccggtg ccggctggcg gttgcagcag
      961 acatcggttg tcggcgcttc tcccgtcgcc ttcggctatg cgggtgaggg ccaaacctcg
     1021 aacctggtcg cggtggtcag tttcgtgggg cgctcgcgtc ccaccggggc gcggatcgtg
     1081 ctgcgcgacc cgaacaaggc gccgcgggcc tggctggccg gcgacggtct ggttcccgct
     1141 tcctcgcgcg ccagtctgtg ggccgcgaac gtcgcgccgg cggaacgcga actgctggct
     1201 gaaggccgcc gcgcggggga ggtctggcgc ttccccgcct cgacggccgc cgcgctggaa
     1261 cggctggacc cgcgcgaaac cttcgccatc gagttccact tccgcgacgg cagcgtggcc
     1321 accgcgatgt tcgaggccgg agacttcgcc gcaggccggg ccttcctggc gatgggaatg
     1381 ctctaggcct caggccccgt tcttcatcgg catcggaatg atctcggccc cgccttccgg
     1441 ggcaggggcg cgggcgacca gagcgaccgg caaccggacc gtgaccgccg tgccctcgcc
     1501 caaggtgctg tcgatgctca tccggccgcc gtgcagttcg gcgaaggcgc ggacgaggga
     1561 caggcccagg cccgtgccct gacggcgctg ctcgatctcg ccggcctgtt cgaaggggcg
     1621 gcccagacgg cgcacgtctt ctggggcgat gcccacgccg gtgtcggcga cgacgacctc
     1681 cagatagggg ccgatagcct cgacagtgac ggtgatcgtt ccacccggcg gggtgaattt
     1741 caccgcattg gacagcaggt tgagagtgat ctgtttcagc gcccgccggt cggccgagac
     1801 ctcgaccgga tcgccgggca ggacgctgga caggctgacg cccttgtcgt cggcgttgac
     1861 ccggaccagc gccacggccg cggacaccgt ctcacgggcg tcgaaccgct cgagcgtcag
     1921 ggcgtagcgt tcggcctcga tcttggcgac gtccagcagg tcgttgatca ggtcaagcag
     1981 atggccgccg gcctggtgga tgttctcggc gtattcgccg tagcggtcgg gcaggggccc
     2041 gaagatgcgc tggcgcatga tgtccgagaa gccgagcacg gcgttcaggg gcgtgcgcag
     2101 ttcgtggctc atattggcca gaaaacgggt cttgccggct tcgcgcgtct cggcctctgt
     2161 gcgggcgacc tccaacccc
//
LOCUS       DASVVN010000113         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_372794,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000113 DASVVN010000000
VERSION     DASVVN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372794"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VF686_01760"
     CDS             complement(<1..672)
                     /locus_tag="VF686_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8660562.1"
                     /translation="MSLALSLTLAACATPAIQPPLIPPPGFAGPALEAQALVMDDGGR
                     LPLARWTPDRAEPWAVIVALHGMNDSRASFRLAGPWWAERGIETWAVDQRGFGEAPGR
                     GVWAGQARMTEDLRTAVALARARHPRAVIAVAGESMGGAVAVAAFGSDRPPAADRVIL
                     LAPAVWGWTAQGPVNSAGLWIAARAMGDRAVGAPEWAVRDLPASDNRVELIRNGRDPN
                     SILPTR"
     gene            complement(768..1397)
                     /locus_tag="VF686_01765"
     CDS             complement(768..1397)
                     /locus_tag="VF686_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8660563.1"
                     /translation="MSLDRVLSDIRACRACLGQLPHTPRPVVRVFPGTRLLICGQAPG
                     RRVHESGLPFTDPSGDRLRQWLGVDYESFYADRRLGVAAQAFCYPGTAPKGGDYPPPR
                     RCAELWRPRLLAELPDVELTLLVGGYAQVWALGDRAKINMTETVRAWRDYAPSILPLP
                     HPSWRNTAWLRKNPWFEEEVLPYLRERVAAILAPSISVHRREGGDPGFS"
     gene            complement(1540..1860)
                     /locus_tag="VF686_01770"
     CDS             complement(1540..1860)
                     /locus_tag="VF686_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HEX8660564.1"
                     /translation="MSDPIETAIFEKLAKADPKGVGGKSIEPADVAKELQPEQWQRML
                     PKVRHCALGLMRQGRLTITKKGKPIDPNAFKGVIRLRLPNEAETAAALAALPPVVEDD
                     DDFA"
     gene            1909..2343
                     /locus_tag="VF686_01775"
     CDS             1909..2343
                     /locus_tag="VF686_01775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8660565.1"
                     /translation="MVEKVTVTEGEFAGWQTYDLHGTFDQVVGPFYFKPDPDGRMRCA
                     FRAEPKHMNAGHRMHGGCLMTFADISLFQTAYQEMEGASGVTVSLESTFIDGAYEGEL
                     VESTGEVVRAGKSLIFVRGQITTGDRILMTYSGVIRKFEKRG"
BASE COUNT          380 a          789 c          860 g          360 t
ORIGIN      
        1 ccgggtcggc aggatgctgt tcgggtcgcg gccattgcgg atcagttcga cgcggttgtc
       61 cgacgccggg agatccctca cggcccattc cggcgcgccg accgcgcggt cgcccatggc
      121 gcgggcggcg atccacaggc cggcggaatt gaccggtccc tgcgcggtcc agccccagac
      181 cgccggggcg agaaggatga cgcgatcggc cgccggagga cggtccgagc caaaggcggc
      241 gacggcgacc gcgccgccca tgctttcccc cgcgacggcg atgacggcgc ggggatgacg
      301 cgcccgggcc agggcgacgg cggtgcgcag gtcctcggtc atccgcgcct gtccggccca
      361 gacgccgcgg ccgggcgcct cgccgaagcc gcgctggtcg acggcccagg tctcgatgcc
      421 ccgctcggcc caccagggtc cggcgaggcg gaaggaggcg cggctgtcgt tcatgccgtg
      481 cagggcgacg atgacggccc acggctcggc ccggtccggc gtccagcggg ccagaggcag
      541 gcgcccgccg tcgtccatga ccaaggcctg cgcttccaga gccggtccgg cgaaacccgg
      601 cggcgggatc aggggcggct ggatggcggg cgtggcgcag gccgccaggg tgagtgacag
      661 cgccagcgac atcccccaat tccgtgcatc cccgcgaagg ggacccagtg ctttggggga
      721 tcgattttcg agcctatgcc cgcacggcaa tcccaacacg gcctgcctca cgaaaaaccg
      781 gggtccccgc cttcgcggcg atgcacggag atagatggag caagaatagc cgcgaccctc
      841 tcgcgaaggt agggcaggac ctcttcctcg aaccacgggt tctttcgcag ccacgccgta
      901 ttgcgccacg acggatgcgg caggggcagg atcgaggggg cgtagtcgcg ccacgcccgc
      961 accgtctccg tcatgttgat cttcgcccgg tcgcccagcg cccagacctg ggcgtagccg
     1021 ccgaccagca gggtcagttc gacatccggc agttccgcca gcagtcgcgg ccgccacaat
     1081 tcggcgcagc ggcgcggcgg cggatagtcg ccgcctttgg gcgccgtgcc cggatagcag
     1141 aaggcctggg cggccacgcc cagccggcgg tcggcgtaga agctctcgta gtcgacgccc
     1201 agccactggc gcagccgatc gccggacgga tctgtgaatg gcagaccgct ctcgtgcacc
     1261 cgccggcccg gggcctggcc acagatcagc agccgcgtcc cgggaaagac gcggaccacc
     1321 gggcgtgggg tgtgaggaag ctggccgagg caggcccggc aggcgcggat gtcggaaagg
     1381 acccggtcca ggctcacatg atcaccgtgc atcccggcga atgccgggac ccagataacg
     1441 aacgcggctc tcgcggcatg agccgccgag ctttctgtac cgcctgacca gattggcatc
     1501 tgggtcccgg cattcgccgg gatgagcgga aaaagtggat caggcgaagt cgtcatcgtc
     1561 ctcgacgacc ggcggcaggg ccgccagcgc cgcggcggtt tcggcctcgt tcggcaggcg
     1621 cagtcggatg acgcccttga aggcgttggg gtcgatcggc ttgcccttct tggtgatggt
     1681 cagcctgccc tgacgcatca ggccgagcgc gcagtgccgc accttgggca gcatccgttg
     1741 ccattgctcg ggctgcagtt ccttggccac gtcggcgggc tcgatgctct tgccgccgac
     1801 gcccttgggg tcggccttcg ccagtttttc gaagatggcg gtttcgatgg ggtcgctcat
     1861 cgggcctgta tggtgcattg cgggatcaga aagaaggcgg agttttccat ggtcgagaaa
     1921 gtcacggtga cggagggcga gttcgccggc tggcagacgt acgatctgca cggcaccttc
     1981 gatcaggtcg tggggccgtt ctacttcaag ccggacccgg acggccggat gcgctgcgcc
     2041 ttccgcgcgg agcccaaaca catgaacgcg ggccaccgga tgcacggcgg ctgcctgatg
     2101 accttcgcgg acatttcgct gttccagacg gcctatcagg agatggaagg cgcctcgggc
     2161 gtgacggtgt cgctggaatc gaccttcatc gacggcgcct atgagggaga gctggtggag
     2221 tcgaccggcg aggtggtgcg ggccggcaag agcctgatct tcgtccgcgg ccagatcacc
     2281 accggcgatc ggattctgat gacctattcg ggcgtgatcc ggaagttcga gaagcgcggc
     2341 tgaaccggcg gtgaccggtg tcagccgtcg acaagatcct gaaggatga
//
LOCUS       DASVVN010000114         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_293437,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000114 DASVVN010000000
VERSION     DASVVN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293437"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2109)
                     /locus_tag="VF686_01780"
     CDS             complement(<1..>2109)
                     /locus_tag="VF686_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8660566.1"
                     /translation="QENNTSQDPVAGVVLGGPAPERTLYATARRLQDRLESVPGVLRA
                     ESNGGREEMLEGTIDPLRMEAYNITPGEMAAVIRSNNQLVPAGNLENAQGRFAVKIPG
                     VIEGPNDVLRLPIKTNGDSVVTLGDVAEVRRTYKEAGSITRIDGERAMVVEAVKRPGA
                     NILETVKQVRAAVDEEQKTWPNGVRASYLYDESEDIAGMLNLLEAGLMIAIVLVMVIV
                     VASLGLRAGLLVGAAIPACFLLAFLMLQAVGITLNMMVMFGMVLAVGMLVDGSIVVVE
                     YADRKMAEGLPKEEAYEAAGERMFWPVFNGIATTLCAFVPFLFWNEIAGKFMSYLPIT
                     LFFVLGASIFVALIVTPSLGSLFGKRSDEHDHAADNIRESEEGDPRRMTGFMGVYARF
                     VAGAIHRPGLMMLAAIAVIVGTFVWFSSTPHRTEFFIDEEPQSVNVFVKARGNLSIEA
                     MDAMVRQVERTVVGVKGVDSAYVRVGRQGGGLFGGAPADTVGRIRLDFLDYEERRAIG
                     LNGNDIRRAVTDAVGEHSGLRVEVQQPQGGPGGGKDLQVELRGADVVALDKAAQTVIA
                     EMRRSDMIDVEDTRTSPGMEWNLTVDREAAGRYGVSVAQVGQAIQFATNGVLVGRFRP
                     DDAEDELDIRVRFPPEARNISAFNQLKIGTALGPIPASYFIKQAPAQQVTTIQRRDGQ
                     RLVLVQANTLEGVAANQKIS"
BASE COUNT          309 a          736 c          719 g          345 t
ORIGIN      
        1 cgctgatctt ctggttcgcc gccacgccct ccagggtgtt ggcctgcacc agcaccagcc
       61 gctggccgtc gcggcgctgg atggtggtca cctgctgggc cggcgcctgc ttgatgaagt
      121 agctcgccgg gatggggccc agcgcggtgc cgatcttcag ctggttaaag gcggagatgt
      181 tgcgcgcctc cggcgggaac cggacgcgga tgtcgagctc gtcctcggcg tcgtcgggcc
      241 ggaagcggcc gaccagcacg ccgttggtgg cgaactggat ggcctggccg acctgcgcca
      301 cgctgacgcc gtagcggccc gccgcctcgc ggtcgacggt gaggttccac tccatgccgg
      361 gcgaggtccg ggtgtcctcg acgtcgatca tgtcgctgcg gcgcatctcc gcgatcaccg
      421 tctgcgccgc cttgtcgagg gcgacgacat cggcgccgcg gagctcgacc tgcaggtcct
      481 tgccgccgcc gggaccgccc tgcggctgct gcacctcgac acgcaggccg gaatgctcgc
      541 cgacggcatc ggtcaccgcg cgtcggatat cgttgccgtt gagcccgatc gccctgcgtt
      601 cctcgtagtc gaggaagtcg agccggatgc gcccgaccgt gtcggccggc gcgccgccga
      661 acaggccccc gccctggcgg ccgacccgca cgtaggcgga gtcgaccccc ttgacgccga
      721 ccacggtgcg ctcgacctgg cgcaccatgg cgtccatcgc ctcgatcgac aggttgccgc
      781 gcgccttgac gaagacgttc acgctctgcg gctcttcgtc gatgaagaac tcggtgcggt
      841 gcggcgtcga gctgaaccag acgaaggtcc cgacgatcac cgcgatcgcc gccagcatca
      901 tcaggccggg ccggtggatc gcgccggcga cgaagcgggc gtaaacgccc atgaagccgg
      961 tcatccgccg cgggtcgccc tcttcagatt cgcggatgtt gtcggcggcg tggtcgtgtt
     1021 cgtcggagcg cttgccgaac agcgagccca gagacggggt gacgatcagc gccacgaaga
     1081 tcgaggcccc gagcacgaag aacagggtga tcggcaggta gctcatgaac ttgccggcga
     1141 tctcgttcca gaacaggaac gggacgaagg cgcacagggt ggtggcgatg ccgttgaaca
     1201 ccggccagaa catccgctcg ccggcggcct cgtaggcctc ctccttgggc agcccctccg
     1261 ccatcttgcg gtcggcgtac tcgaccacca cgatcgagcc gtcgaccagc atgccgacgg
     1321 ccagcaccat gccgaacatg accatcatgt tcagcgtgat gccgacggcc tgcagcatca
     1381 ggaacgccag caggaagcag gccgggatgg cggcgccgac cagcagcccg gcgcgcaagc
     1441 ccaggctggc gacgacgatg accatgacca gcacgatggc gatcatcagc ccggcttcga
     1501 gcagattaag catgccggcg atgtcttcgc tctcgtcata gaggtagctg gcgcgcacgc
     1561 cgttgggcca ggtcttctgc tcctcgtcga ccgcggcgcg gacctgcttc accgtctcca
     1621 ggatgttggc gcccggccgc ttgacggcct cgaccaccat cgcgcgctcg ccgtcgatgc
     1681 gggtgatcga gccggcttcc ttgtaggtgc ggcgcacctc ggccacgtcg ccgagggtga
     1741 ccacgctgtc gccattggtc ttgatcggca ggcgcaggac gtcgttgggg ccttcgatga
     1801 ccccaggaat cttgaccgcg aagcgccctt gggcgttctc caggttgccc gccggcacca
     1861 gctggttgtt ggagcggatc accgcggcca tctcgccggg tgtgatgttg taggcctcca
     1921 tccgcagcgg atcgatggtg ccctcgagca tctcctcgcg gccgccgttg ctctcggccc
     1981 gcagcacgcc gggcacgctc tccagccggt cctgcagtcg ccgcgcggtg gcgtagagcg
     2041 tgcgctcggg cgccggtccg cccagcacca cgccggcgac ggggtcctgg ctggtgttgt
     2101 tctcctgga
//
LOCUS       DASVVN010000115         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_135827,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000115 DASVVN010000000
VERSION     DASVVN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135827"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..556)
                     /locus_tag="VF686_01785"
     CDS             complement(11..556)
                     /locus_tag="VF686_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEX8660567.1"
                     /translation="MRTQLILTTVAVLALALSACSNDEAKAPEASAAAPSGPVGRDAV
                     YAAAEEGPEPFVRALYGQYINGGPQGEPPAPGRDPLYDRMLNAMIGEDFRKAGGEVPT
                     LNYDPICGCQDSGNFTLESVTVIPGADDRKADVAVVFTNMGETKRQTLKLVKEGRMWK
                     VSDVLVPGEKPLTEQLMAAIS"
     gene            complement(568..1077)
                     /locus_tag="VF686_01790"
     CDS             complement(568..1077)
                     /locus_tag="VF686_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660568.1"
                     /translation="MLALSACSSPEATVAGPANAATAEAQTPEAFVRSLYSVDSGGTG
                     EVRLEYDGDLETISSARTAALFAEAKALTPPGYIGYPEGHPLVEYEEFGSLRLDDVVT
                     TRHGPDRADVALVLAFNEPDTRVRRVFRLVKEEGRWRLDDMLFNDGEQSLVEGFNSYI
                     ADAKANPDG"
     gene            1371..>3428
                     /gene="ileS"
                     /locus_tag="VF686_01795"
     CDS             1371..>3428
                     /gene="ileS"
                     /locus_tag="VF686_01795"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX8660569.1"
                     /translation="MADAPKTGEDTPTGRDYRETVFLPETPFPMRGGLPQKEPAIIEQ
                     WGDLYSALRKQRQAEGAPLYVLHDGPPYANGDIHIGHALNKTLKDFVVRSRFLLGNDV
                     DYVPGWDCHGLPIEWKIEERYRAQGKRKDEVSKAEFRQACREYAAGWIDVQREQFKRL
                     GVLGDWDHRYATMDFTTEAAIVGEFHKFKNSGQLYRGSKPVMWSPVERTALADAEIEY
                     HDHVSPTIWVKFPVTGATDDHPDDLLAFRHANPSVVIWTTTPWTIPANRAISYGPEIA
                     YGLYEVTAMEEGLEFQPWAKPGDRLIVADKLAEDVFKTAKIAAWTRIDDLNPSGLECA
                     HPLAALSPGFGYSVPLLAGDHVTDDAGTGFVHTAPGHGADDFEVWKAHGHHEVPDTVD
                     PDGAYYDHVPLFAGLKVLETEGKKTGKFGPANGAVMDKLIEAGNLLARGRMEHSYPHS
                     WRSKAPIIFRNTPQWFIRMDAPLKGDGTLRERALEAIDKTDFHPEAGRNRIRSMVESR
                     PDWLISRQRAWGTPLAMFVDKQTGQPLVDAEVDSRITAAIAEGGADAWFTRPDADFLG
                     HHDASRYEKIEDILDVWFDSGCTHAFTLETRDRAHGYTGDRPSHWPADLYLEGSDQHR
                     GWFQSNLMEGAGTRGRAPYDAVLTHGFTQDENGEKMSKSRGNTTDPAVVIKESGADIL
                     RLWV"
BASE COUNT          589 a         1292 c         1026 g          521 t
ORIGIN      
        1 cgcgccgtcc tcagctgatc gcggccatca gctgctcggt cagcggcttt tcgccgggaa
       61 cgaggacgtc gctgaccttc cacatccgcc cctccttcac cagcttcagt gtctgccgct
      121 tcgtctcgcc catattggtg aacaccaccg ccacatcagc cttccggtcg tccgcgcccg
      181 gaatgaccgt gacggactcc aaggtgaagt tcccgctatc ctgacagccg cagatggggt
      241 cgtagttgag cgtcgggacc tctccgcccg ccttgcggaa atcctctccg atcatggcgt
      301 tcagcatccg atcgtagagc ggatcccgcc ccggcgccgg cggctcgccc tgcggtccgc
      361 cattgatgta ctggccgtag agcgcccgca cgaacggctc cggcccttcc tcggcggcgg
      421 catagaccgc gtcgcggcca acgggcccgc tcggcgcggc ggccgacgcc tccggcgcct
      481 tggcctcgtc gttcgaacag gcggacagcg ccagcgccaa aaccgccacc gtcgtcagga
      541 tcagttgcgt cctcatcagt cctctcctca cccgtccgga ttcgccttgg cgtcggcgat
      601 atagctgttg aatccttcga cgaggctctg ctctccgtca ttgaacagca tgtcgtccag
      661 ccgccagcgt ccctcctcct tcaccagcct gaacacccgt ctcacccgcg tatccggctc
      721 gttgaacgcc agaaccagcg ccacatcggc ccgatccggc ccgtgccgcg tcgtgacgac
      781 atcgtccagc cgcagcgatc caaactcctc atattccacg agcggatgcc cttcgggata
      841 gccgatatac cccggcggcg tcagcgcctt ggcttcggcg aacaaggcag ccgtgcgtgc
      901 agaactgata gtctccaggt cgccgtcgta ctcgagacgg acttcgccag tgccgcccga
      961 gtcgacgcta tagagcgacc gtacaaaggc ttcgggcgtc tgcgcctcgg cggtcgcggc
     1021 attcgcgggt cccgccacgg tcgcctcggg cgatgaacag gcggacaggg ccagcacggc
     1081 ggcggctatc agaacgtggc gcatgggttg gccccctccc cgggacagcc gactattggg
     1141 ccgccggccc ggcatggcaa tcccgctggc gtccggcccc cgccgtctgc tagaccgtac
     1201 ccaccatgac cgtccgcgtg tttcgcccga tttcgattag ccgcccggcg tagcgccgca
     1261 gggctctggc cccgtgcacg ccgggataac gacacgcgct caagcaggcc tccggctggt
     1321 agcgcatcga aaatctacgc gaacgatcac ccacgacgag acgagacttc atggccgacg
     1381 cccccaagac cggtgaagac acgcccaccg gacgcgatta ccgcgagacg gtcttcctgc
     1441 ccgagacgcc cttccccatg cgcggcggcc tgccgcagaa ggaaccggcg atcatcgagc
     1501 agtggggcga cctctattcc gccctgcgca aacagcgcca ggccgagggc gcgcccctct
     1561 acgtcctgca cgacggcccg ccctacgcca acggcgacat ccacatcggc cacgccctca
     1621 acaagacgct gaaggacttc gtcgtccgca gccgcttcct gctcggcaac gacgtcgact
     1681 acgtccccgg ctgggactgc cacggcctgc cgatcgagtg gaagatcgag gaacgctacc
     1741 gcgcccaggg caagcgcaag gacgaggtct ccaaggccga gttccggcag gcctgccgcg
     1801 aatacgccgc cgggtggatc gacgtgcagc gcgagcagtt caagcggctg ggcgtcctgg
     1861 gcgactggga ccaccgctac gccaccatgg acttcaccac agaggcggcc atcgtcggcg
     1921 agttccacaa gttcaagaac tcgggccagc tgtaccgcgg ctccaagccc gtgatgtggt
     1981 cgccggtcga gcgaaccgcc ctggccgacg ccgagatcga ataccacgac cacgtcagcc
     2041 ccacgatctg ggtcaaattc cccgtcaccg gcgcgactga cgatcatccc gacgacctgc
     2101 tggccttccg ccacgccaat ccgtcggtcg tcatctggac caccacgccc tggaccatcc
     2161 cggccaaccg cgccatcagc tacgggcccg agatcgccta tggcctctat gaggtcacgg
     2221 ccatggaaga gggtctcgag ttccagccct gggccaagcc gggcgaccgc ctgatcgtcg
     2281 cggacaaact ggccgaggac gtcttcaaga ccgcgaagat tgcggcttgg acccgcatcg
     2341 acgacctgaa cccttcgggc ctcgagtgcg cccacccgct ggcggccctg tcgcccggct
     2401 ttggctattc cgtgcccctg ctggccggcg atcacgtgac cgacgacgcc ggcaccggct
     2461 ttgtccacac cgcgcccggc cacggcgccg acgacttcga ggtctggaag gctcacggcc
     2521 accacgaggt gcccgacaca gtcgatcccg acggcgccta ctacgaccac gtccccctgt
     2581 tcgcgggcct caaggtgctg gagaccgagg gcaagaagac cggcaagttc gggcccgcca
     2641 acggcgcggt catggacaag ctgatcgagg ccggcaacct gctggcgcgc ggccggatgg
     2701 agcattccta tccgcacagc tggcgttcca aggccccgat catcttccgc aacacgcccc
     2761 agtggttcat ccgcatggac gcgccgctca agggcgacgg caccctgcgt gagcgcgcgc
     2821 tcgaggccat cgacaagacc gacttccacc ccgaggccgg ccgcaaccgc atccgctcca
     2881 tggtcgagag ccggcccgac tggctgatca gccgccagcg cgcctggggc acgccgctcg
     2941 ccatgttcgt cgacaaacag accggccagc cgctggtcga cgccgaggtc gactcccgca
     3001 tcaccgcggc catcgctgag ggcggcgccg acgcatggtt cacccgcccc gacgccgact
     3061 tcctcggcca tcatgacgcc tcccgctacg agaagatcga ggacattctc gacgtttggt
     3121 tcgacagcgg ctgcacccac gccttcacgc tggagacgcg cgatcgggcc cacggctaca
     3181 cgggcgaccg cccgtcgcac tggcccgcgg acctctatct cgaaggctcg gaccagcacc
     3241 gcggctggtt ccagtccaat ctgatggagg gcgccggcac ccgcggccgc gccccctatg
     3301 acgccgtcct gacccacggc ttcacccagg acgagaacgg ggagaagatg tccaagtcgc
     3361 ggggcaatac gaccgatccc gccgtcgtca tcaaggaatc cggcgccgac atcctgcgcc
     3421 tgtgggtc
//
LOCUS       DASVVN010000116         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_110416,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000116 DASVVN010000000
VERSION     DASVVN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110416"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF686_01800"
     CDS             complement(<1..320)
                     /locus_tag="VF686_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX8660570.1"
                     /translation="MILVVDNYDSFTYNLVHYLAELGAETRVVRNDDLTAAEAWALKP
                     EAVLLSPGPCTPNEAGICLSVLDSAPLDMPIFGVCLGHQAMGQAFGGEVIRAKALMHG
                     KTSP"
     gene            427..609
                     /locus_tag="VF686_01805"
     CDS             427..609
                     /locus_tag="VF686_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8660571.1"
                     /translation="MTDKTIARARVLLTPVAAKANPYAALGAAAMAAMAAVLMAGVVV
                     LGPGVRFDEPIVSAEP"
     gene            complement(606..>1577)
                     /locus_tag="VF686_01810"
     CDS             complement(606..>1577)
                     /locus_tag="VF686_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX8660572.1"
                     /translation="YPDGGSDADTAFAAAVRRLDAFEADLRTPLPAIPAAIEATVADF
                     ASPVDGAGFGELVARAKDYIAAGDIFQVVLSHRFAAPWSADPFAFYRSLRRSNPSPYL
                     FFLDLGDFQLAGSSPEILVRLKDGRVTIRPLAGTRPRGETPEADRALEAELLADPKER
                     AEHLMLLDLGRNDVGRVAAPGTVEVIESFRIERYSSVMHIVSEVQGAADPKLDAVDTL
                     LAALPAGTLSGAPKVRAMEIIDELETTKRGVGYGGGVGYISAGGEADICIVLRTALFA
                     DGKIHVQAGAGVVADSDPAAEYAETKAKARAPMRAAEDAWRYPFGNR"
BASE COUNT          240 a          508 c          589 g          240 t
ORIGIN      
        1 atcggcgagg tcttgccgtg catcagggcc ttggcgcgga tcacctcgcc gccgaaggcc
       61 tgacccatgg cctgatgccc gaggcagacg ccgaagatcg gcatatccag cggggcgctg
      121 tccagaacgg acaggcagat accggcttcg ttcggcgtgc aggggccggg cgatagcagg
      181 acggcctcgg gtttcaaggc ccacgcttcg gccgcagtca ggtcatcgtt gcgcaccacg
      241 cgcgtctccg cgcccaactc ggccaggtag tggacgaggt tgtaggtaaa gctgtcgtag
      301 ttatcgacga ccaggatcat ggtctggctt ctagggggtg tggggcggcg cgccaagcgc
      361 atcgggcgcg taagtcttcg ttaaccaaac tgtcccgaag tggggcggtc caacggggag
      421 ccgggcatga ccgacaagac catcgccaga gcccgcgttc tgctgacgcc cgtggccgcg
      481 aaagccaatc cctacgccgc tcttggcgca gccgcgatgg cggcgatggc ggcggtgctg
      541 atggcgggcg tggtcgtgtt ggggcccggc gtgcggttcg acgagccgat cgtctcggcc
      601 gagccctagc ggttgccgaa cggataacgc caggcgtctt cggcggctcg catcggcgcg
      661 cgggccttgg ctttcgtctc ggcgtattcg gcggccggat cgctatcggc cacgacgccg
      721 gcgccggcct ggacgtgaat cttgccgtcg gcgaacaggg cggtgcgcag gacgatgcag
      781 atgtcggcct cgcccccggc tgagatgtag ccgacgccgc cgccgtaacc gacgccgcgc
      841 ttggtggtct ccagctcgtc gatgatctcc atcgcccgga ccttgggcgc gccggacagg
      901 gtgccggcgg gcagggcggc cagcagggtg tcgacggcgt ccagcttcgg atcggcggca
      961 ccttggacct cggacacgat gtgcatcacc gagctgtagc gctcgatacg gaagctctcg
     1021 atgacctcga cggtgcccgg cgcggcgacg cggccgacat cgttgcggcc cagatccagc
     1081 agcatcagat gctcggcccg ctccttggga tcggcgagca gttcggcctc gagggcgcgg
     1141 tcggcctcgg gcgtctcgcc gcgcgggcgg gtgccggcca gcgggcggat ggtgacgcgg
     1201 ccgtccttga gccggacgag gatctccggg ctcgatccgg ccagctggaa gtcgccgagg
     1261 tcgaggaaga acagataggg cgagggattc gaccggcgca gcgagcggta gaaggcgaac
     1321 ggatcggcgc tccatggcgc tgcgaaacgg tggctgagca cgacctggaa gatgtcgccg
     1381 gcggcgatgt agtccttggc tcgcgcgacc agctcgccga agccggcccc gtcgacgggc
     1441 gaggcgaagt ccgcgacggt cgcctcgatc gctgctggaa tcgcgggcag gggcgtgcgc
     1501 aggtcggcct cgaacgcgtc cagccgccgg accgccgccg caaaagccgt gtcggcgtcc
     1561 gagccgccgt cgggata
//
LOCUS       DASVVN010000117         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_241220,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000117 DASVVN010000000
VERSION     DASVVN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241220"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VF686_01815"
     CDS             <1..1238
                     /locus_tag="VF686_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX8660573.1"
                     /translation="EAQIDEAVRRLLTMKFQSGVFEQPYVDARAAERRTATPDAIALA
                     REAAQRSMILLKNEGGLLPLNAGRIRKLAVLGTHARDTPIGGYSEVPKHVVSVLEGIE
                     AEGRGKFEVVYSEAVRITESRIWAADTVTLVDPAVNARLIEQAVRDARDADVIVMVLG
                     DNEQTSREAWADGHLGDRDSLELIGQQNDLARAIFALNKPTVVLLLNGRPLSVNYVTE
                     HAAALIEGWYLGQETGRAVADVLFGRVNPGGKLPVSVARDVGQLPIFYNHKPTSRRGY
                     LLGETKPLFPFGYGLSYTTFDISEPRLARASIRNGETVRVEVDVRNTGPVKGDEVVQL
                     YVRDDHASVTRPVKELKHFRRVTLEPGARTTVAFEISPRDLWFWNIDMERVVEPGTFT
                     LMAGPNSVDLKTATLTVTA"
     gene            complement(1235..>1784)
                     /locus_tag="VF686_01820"
     CDS             complement(1235..>1784)
                     /locus_tag="VF686_01820"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8660574.1"
                     /translation="FDGARLAPSWMMMRNPRETWFDLTSHPGALTLRMRPEGLGDNAN
                     PSFLARRQQHQRATAETTLREVPAREGDRAGLAVLQSDDFWYAMAAVRRDGRPVIELR
                     RRRGAETDKAGEVIAIAPLTGEGPVRFRITARAGAYDFAFTRGEAEWTNVLADADGAM
                     LSTRVAGGFVGAVFGPFAQTGP"
BASE COUNT          286 a          609 c          626 g          263 t
ORIGIN      
        1 ccgaggccca gatcgacgag gcggtgcgca ggctgctgac aatgaaattc cagtcgggcg
       61 tgttcgagca accgtacgtc gacgcccgcg cggccgaacg gcggaccgcc acgcccgacg
      121 ccatcgccct ggcgcgcgag gcggcccagc ggtcgatgat cctgctcaag aacgagggcg
      181 gcctgctgcc cctgaacgcg ggccggattc gcaagctggc ggtgctgggg acccatgcgc
      241 gcgacacgcc gatcggtggc tattccgagg ttccaaaaca cgtcgtcagc gtgctggagg
      301 gcatagaggc cgaggggcgc ggcaagttcg aggtggtcta ttccgaagcg gtccggatca
      361 ccgaaagccg tatctgggcg gccgacacag tcaccctggt cgatccggcg gtcaatgcgc
      421 ggctgatcga acaggcggtc cgggacgcgc gggacgccga cgtcattgtc atggtgctgg
      481 gagacaatga gcagaccagc cgcgaggcct gggccgacgg ccatctgggc gatcgggact
      541 cgctggagct gatcggccag cagaacgatc tggcccgcgc catcttcgcc ctgaacaagc
      601 cgaccgtagt gctgctgctg aacgggcggc ccctgtcggt gaactatgtc accgagcacg
      661 ctgcggccct gatcgagggc tggtacctcg gccaggagac cggtcgcgcg gtcgccgacg
      721 ttctgttcgg ccgcgtcaat ccgggcggca agctgcccgt gtcggtggcc cgcgacgtcg
      781 gtcagctgcc gatcttctac aaccacaaac cgacgtcccg gcgcggctat ctgctcggcg
      841 agaccaagcc cctgttcccg tttggctacg gcctcagcta caccaccttc gacatctccg
      901 aaccgcgcct ggcgcgcgcc agcatccgca acggcgagac ggtccgggtc gaggtggatg
      961 tccgcaacac gggcccggtc aagggcgacg aggtggttca gctctatgtt cgcgacgacc
     1021 acgcctcggt gacgcggccg gtcaaggaac tgaagcattt ccggcgcgtg accctggaac
     1081 ccggcgcccg gaccaccgtg gcgttcgaga tcagcccgcg ggacctgtgg ttctggaaca
     1141 tcgacatgga gcgggtggtc gagccgggga cctttaccct gatggccggc ccgaactccg
     1201 tcgacctgaa gacggcgacc ctgacggtta ccgcctaggg gccggtctgg gcgaaggggc
     1261 cgaagacggc gccgacgaaa ccgcccgcga cgcgggtgct cagcatcgcg ccatccgcgt
     1321 cggccagcac gttcgtccac tccgcctcgc cgcgggtaaa ggcgaagtca taggcgccgg
     1381 cccgggccgt gatgcggaac cgcaccggcc cctcgccggt cagcggagcg atcgcgatga
     1441 cctctccggc cttgtccgtc tccgcacccc ggcggcggcg aagttcgatc accggacgcc
     1501 cgtcgcgccg tacggcggcc atggcgtacc agaagtcgtc gctctgcagc acggccagtc
     1561 cggcgcggtc accctcgcgc gcgggcacct cgcgcagggt cgtctcggcc gtcgcccgct
     1621 gatgctgctg gcggcgggcg aggaaggacg ggttggcgtt gtcgcccagt ccttcggggc
     1681 gcattcgcag ggtcagggcg ccggggtggc tggtcaggtc gaaccaggtc tcgcgcggat
     1741 tgcgcatcat catccaggac ggggccagac gcgcaccgtc gaac
//
LOCUS       DASVVN010000118         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_399403,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000118 DASVVN010000000
VERSION     DASVVN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399403"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF686_01825"
     CDS             <1..297
                     /locus_tag="VF686_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8660575.1"
                     /translation="VGGPVLDLYRGGTDESTFGPRLVLTGSTFERSGRSGDEAGDLKL
                     YGDQRAEVRDNHFIDSGAPRFFRRVGEPVFVWSGNRLENTPALITNVPPLETAP"
     gene            294..>1837
                     /locus_tag="VF686_01830"
     CDS             294..>1837
                     /locus_tag="VF686_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX8660576.1"
                     /translation="MNRYVLTAVLACLAAAVPAGALAQAAPAMVSERADDASPVLVSA
                     DQWRAMAEAGDRYPMFAAERRRVEGEVRAAMAAGVNVPQPRDLGGGVTHEQHKANYKA
                     IAGAGALYRMTGDRAYADFARDLLLEYARLYPTLSNHPAGRSSVKGRLFWQALNDSVW
                     LVYAAQGYDAIRDTLSAEQRRLIDDDVFRRMARFLSEENAPYFDRIHNHATWATAGVG
                     MTGYVLRDPELVERALNGTARDGRAGFLRQVDELFSPDGYYAEGPYYQRYALAPFVIF
                     ARAIQQNEPQRDIFGRRDGVLLKAVDSVIQASYAGRLFPINDALPDKGLDSEEIVTGV
                     AIAYARTQDSVLLSIAQRQGRTLLSPDGLAVAQGVATGAVQPFDFRSMQLRDGPNGDL
                     GNLVFLRRGGENGQALVMKNTQQGQGHGHFDRLNWLFYDNGNAVVTDYGAARFLNIEA
                     KNGGGYLDENDSWAMQTVAHNTLVVGQASQFGGDWRIGEEHPTTPLVFDTGEGVQIAS
                     ARIDNA"
BASE COUNT          307 a          607 c          635 g          288 t
ORIGIN      
        1 gtgggcggac cggttctgga tctctatcgc ggcggaacgg acgaaagcac cttcggcccg
       61 cgtctggtgc tgaccgggtc cacgttcgaa cgcagcgggc ggagtggcga tgaggccggc
      121 gacctcaagc tctacggcga ccagcgcgcc gaggttcgcg acaaccactt catcgacagc
      181 ggggcgccgc gcttcttccg gcgcgtgggg gaacctgtgt tcgtgtggag cggcaatcgc
      241 ctcgagaaca cgccggcgct catcaccaat gttccgccgc tggagaccgc gccttgaacc
      301 gctatgtcct cacggccgtt ctggcgtgcc tggccgccgc ggttcccgcg ggcgcactgg
      361 cccaggcggc gccggcaatg gtgagcgagc gggccgatga cgcctctccg gtgctggtct
      421 cggcggatca gtggcgcgcc atggccgagg ccggcgaccg atacccgatg ttcgcggcgg
      481 agcgccggcg ggtggagggg gaagtccgcg ccgccatggc cgcgggcgtc aacgtgcccc
      541 agccgcgcga tctgggcggc ggcgtcaccc acgagcagca caaggcgaac tacaaggcga
      601 tcgcgggggc cggcgccctt tatcgcatga ccggggaccg ggcctatgcg gacttcgcgc
      661 gggacctgtt gctggaatac gcgcgcctct atccgaccct gtccaaccat ccggccggcc
      721 gatcgtccgt gaagggccgc ctgttctggc aggcgctcaa cgactcggtg tggctggtct
      781 atgcggcgca gggctatgac gcgatccgcg acacgttgag cgctgaacag cggcgtctga
      841 tcgacgatga cgtcttccgc cggatggcgc ggttcctctc cgaagagaac gccccctatt
      901 tcgaccgcat ccacaaccac gccacctggg ccacggctgg cgtcggcatg accggctatg
      961 tgctgcggga tccggagctg gtcgagcggg cgctgaacgg aaccgccagg gatggccggg
     1021 ccggcttcct gcgccaggtc gacgagctgt tctcgcccga cggatactac gccgaggggc
     1081 cgtactatca acgctacgcc cttgcgccct tcgtcatctt cgcccgcgcg atccagcaga
     1141 acgagccgca acgagacatc ttcggacgtc gagacggggt cctgctgaag gccgtcgaca
     1201 gcgtcatcca ggcttcctat gcgggccggc tgttcccgat caacgacgcc ctgcccgaca
     1261 agggactgga cagcgaagag atcgtcaccg gcgtggccat cgcctacgcc cggacgcagg
     1321 acagcgtcct gctgtcgatc gcgcagcggc aagggcgaac ccttctttcg cccgatgggc
     1381 tggccgtggc gcaaggcgtg gcgaccggag ccgtccagcc gttcgatttc aggtcgatgc
     1441 agctgcgcga cggtccgaac ggcgacctcg gcaatctggt cttcctgcgt cgcggcggag
     1501 agaacggcca ggcgctggtg atgaagaaca cccagcaggg tcagggccac ggccatttcg
     1561 accggctgaa ctggttgttc tacgacaacg gaaatgcggt cgtcacagac tatggcgcgg
     1621 cccggttcct caacatcgaa gccaagaacg gcggcggtta tctggacgag aacgacagct
     1681 gggcgatgca aaccgtcgcc cacaatacgc tggtggttgg tcaggccagc cagttcggcg
     1741 gcgactggcg gatcggcgaa gagcatccga cgaccccgct ggtcttcgat acaggggagg
     1801 gggtgcagat cgcctcggcc cggatcgaca acgcgta
//
LOCUS       DASVVN010000119         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_30245, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000119 DASVVN010000000
VERSION     DASVVN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30245"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VF686_01835"
     CDS             <1..1882
                     /locus_tag="VF686_01835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8660577.1"
                     /translation="RSGSLHIMDLRDGGEGRRLAISEGGANTARWGSDGRLYFLSGRS
                     GSSQVWRANADGTGPVQVTDLPLDVNAYRLSPGADKVAVSLAVFPECGDLACTVDRAK
                     AVADDPATGQVYDRMFVRHWDTWNDGTQNHLFVQSIGANGRATGTPVHVTKGFDGDTP
                     SKPFGDESEFTFAPDGQSIVFSARLAGQTEPWSTNFDLWRTNGLSGDGTFENLTDAND
                     AWDTGPVFSPDGRTMAYRAMARPGFEADKLAIFLRDVATGQTRQIAADWDRSADTLQW
                     SRDGNTIYVTAGDVGQTRIFSIDTRNGVVTPVTGPGHVSAFAQTPSGFVYAQDTLTRP
                     SELFVKTFRGREMPRQITNVNPQLNDQAFGEPEQFSFTGWNGETVYGHVIKPANYVEG
                     QKYPVAFLIHGGPQGSFGNGWSYRWNPETYAGAGYAVVMIDFHGSTGYGQAFTDSISQ
                     HWGDRPLEDLQKGWAAAQERYGFLDGDNACALGASYGGYMINWIAGKWSDEFKCLVNH
                     DGVFDIRGMAYGTEELWFTEWEYGGTHYDNPRGYEAFNPVHHVDQWKTPMLVVQGDLD
                     FRIPTSQGLSTFTALQRRGVESRLLFFPNENHWVLKPANSLQWHNEVFGWLDKYLKPA
                     EQ"
     gene            2009..>3178
                     /locus_tag="VF686_01840"
     CDS             2009..>3178
                     /locus_tag="VF686_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX8660578.1"
                     /translation="MPNPLKDHPNWLVVRDRALVPAGAWAKDRRDWIRANDAVYTSFR
                     RPGRPEQIAASVTLLLITAIVVFLMLFDWNWLRGPIGRWASEKYDREIVLQGDLDVKL
                     FSWTPSAHIRDARVGGPDWAKEKDTLVVEDALASVRFLPLLSGQVEMPLVEITRPEIV
                     LISTADGRKSWVLNPDKPDTGPMKLPPINRLVIRDGAVSLDEQGRNIRLNATINAREG
                     TDGNAGFHLDGRGTINGTPLKLEVRGGPFINIKRNQPYGFKAELSGVGTTLIADGSIT
                     RPFDLGQFNATLSLRGRDLADIYLLTGITTPNTPPYRLSGKLTRDDTVFTFNDFSGRV
                     GSSDLSGDLKVYKTGDRRQVEAQLYSRLLDIDDLASVLGGTPRVTPSGNTVVTSGV"
BASE COUNT          542 a         1103 c         1042 g          491 t
ORIGIN      
        1 ccgcagcggt tcgctccaca tcatggacct gagagatggg ggcgagggcc gccggctggc
       61 gatcagcgag ggcggggcca acaccgcgcg ctggggttcg gacggcaggc tgtatttcct
      121 gtcgggccgc tcgggctcca gccaggtctg gcgcgccaac gccgacggga ccggcccggt
      181 gcaggtcacg gacctgccgc tggacgtcaa cgcctaccgg ctgagcccgg gcgcggacaa
      241 ggtggcggtg tcgctggccg tcttccccga atgcggcgac ctcgcctgca cggtggaccg
      301 cgccaaggcc gtggccgacg atcccgcgac ggggcaggtc tatgaccgga tgttcgtgcg
      361 ccactgggac acctggaacg acggcaccca gaaccacctc ttcgtccagt cgatcggcgc
      421 caacggccgc gccacgggga cgccggtcca tgtgacgaag ggcttcgatg gcgacacccc
      481 ctccaagccg ttcggcgacg agagcgaatt caccttcgcc ccggacggtc agtcgatcgt
      541 cttctcggcc cggctcgccg gccagaccga gccgtggagc accaatttcg acctgtggcg
      601 caccaacggc ctgtcgggcg acggcacgtt cgagaatctg accgacgcca acgacgcctg
      661 ggacaccggc ccggtgttct cgccggacgg ccgcacgatg gcctaccgcg ccatggcccg
      721 cccgggcttc gaggccgaca agctggcgat cttcctgcgc gacgtggcga cgggccagac
      781 gcgccagatc gcggccgact gggaccgctc ggccgatacc ctgcagtggt cgcgcgacgg
      841 gaacaccatc tacgtcaccg ccggcgacgt cggccagacg cgcatcttct ccatcgacac
      901 ccgcaacggc gtggtcacgc cggtcacggg accgggacac gtctcggcct tcgcccagac
      961 gccgtccggc ttcgtctacg cccaggacac gctgacccgt cccagcgagc tgttcgtcaa
     1021 gaccttccgc ggccgcgaaa tgccccggca gatcaccaat gtgaacccgc agctgaacga
     1081 ccaggccttc ggcgagcctg agcagttcag cttcaccggc tggaacggcg agaccgtcta
     1141 cggccatgtc atcaagccgg ccaactacgt cgagggacag aagtatcccg tcgccttcct
     1201 gatccacggc ggaccgcagg gctcgttcgg caacggctgg tcctaccgct ggaatcccga
     1261 gacctacgcc ggcgccggct atgcggtggt gatgatcgat ttccacggct cgaccggcta
     1321 cggccaggcc ttcacggact cgatcagcca gcactggggc gaccgtccgc tggaggatct
     1381 gcagaagggc tgggccgccg cgcaggagcg ctacggcttc ctcgacggcg acaacgcctg
     1441 cgccctcggc gcctcatacg gcggctatat gatcaactgg atcgccggca agtggtcgga
     1501 cgagttcaaa tgcctggtca atcacgacgg cgtcttcgac atccgcggca tggcctacgg
     1561 caccgaggag ctgtggttca ccgagtggga atacggcggc acccactacg acaacccgcg
     1621 cggctatgag gccttcaatc cggtccatca cgtcgaccag tggaagacgc cgatgctggt
     1681 ggtgcagggc gacctcgact tccgcatccc gacctcgcag ggcctgtcga ccttcaccgc
     1741 cctgcagcgg cgcggcgtcg agagccggct gctgttcttc ccgaacgaga accactgggt
     1801 gctgaagccg gccaacagcc tgcaatggca caatgaggtg ttcggctggc tggacaaata
     1861 tctgaagcct gccgagcagt agactgaacg acacaagggg cgcgtccgga tcgggcgcgc
     1921 cctttttgcc gctcgcgcgg gcaatcgcgg cccgacctga gccttccgcc gctttgcgcg
     1981 ttgtggaggg ttcgacggag taccgcgtgt gcccaacccc ttgaaagacc atcccaactg
     2041 gctggtggtg cgcgaccgcg ccctggtgcc ggcgggcgcg tgggcgaagg acaggcgcga
     2101 ctggatcagg gccaacgacg ccgtctacac cagcttccgc cgccccggcc gacccgagca
     2161 gatcgcggcc agcgtcaccc tgctgctgat cacggcgatc gtcgtcttcc tgatgctgtt
     2221 cgactggaac tggctgcgcg ggccgatcgg ccgctgggct tcggagaagt acgaccgcga
     2281 gatcgtgctg cagggcgatc tggacgtgaa actgttcagc tggacgccgt cggcccacat
     2341 ccgagacgcg cgcgtcggcg gacccgactg ggcgaaggag aaggacaccc tggtcgtcga
     2401 ggacgccctc gcctcggtgc gctttctgcc gctgctgtcg ggacaggtcg agatgccgct
     2461 ggtcgagatc acccggccag agatcgtgct gatctcgacc gccgacggcc gcaagagctg
     2521 ggttctgaac ccggacaagc ccgataccgg cccgatgaaa ctgccgccga tcaacaggct
     2581 ggtcatccgc gacggcgcgg tctctctcga cgaacaggga cgcaacatcc gcctgaatgc
     2641 gaccatcaat gcgcgcgagg gcacggacgg gaacgccggc ttccatctgg acgggcgcgg
     2701 cacgatcaac ggcacgccgc tgaagctcga ggtgcggggc gggcccttca tcaatatcaa
     2761 gcggaaccag ccttatggtt tcaaggccga gctttcgggc gtgggcacca ccctgatcgc
     2821 ggacggctcg atcacccggc cgtttgatct gggccagttc aatgcgacgc tcagcctgcg
     2881 cggccgcgat ctggcggaca tctatctgct gaccggcatc accacgccca atacgccgcc
     2941 ctatcggctg agcggcaagc tgacccgcga cgacaccgtc ttcaccttca acgacttcag
     3001 cggccgggtg ggttcctccg acctgtcggg cgatctgaag gtctacaaga ccggtgaccg
     3061 gcggcaggtc gaggcgcagc tctattcccg gctgctggac atcgacgacc tcgcgtccgt
     3121 gctgggcggc accccgcgcg tgaccccctc cggtaatacg gttgtgacgt cgggcgtc
//
LOCUS       DASVVN010000120         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_477607,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000120 DASVVN010000000
VERSION     DASVVN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477607"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VF686_01845"
     CDS             complement(<1..1188)
                     /locus_tag="VF686_01845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8660579.1"
                     /translation="MAEDAIEGGATNAWQEAVIDKLPPAEALAGVEREVMEYDVVIVG
                     GGPAGLAAAIRLKQRAEKDGKDITVAVLEKSAEIGGHILSGAVIDPRALNELFPDWKE
                     RGAPLETPVTEDRFLVLGPMGQASLPMALLPPMMHNDGCYIASLGNVARWLGEQAEAL
                     GVEVYPGMAASHVVWDEPTGRVKGVVAGVFGIDRHGKPTGDFQPGIELHGKYVFIAEG
                     VRGSLAKTIMARHNLCDDAQPQKFGIGLKELWQVPADKHRPGLAQHTTGWPLDEFTGG
                     GSFMYHFGDRYVAIGYVVHLNYKNPFLSPFDEFQRFKHHPSIAEHLEGGTRISYGARA
                     ITEGGFQSVPKLTFPGGVLIGDSAGFVNVPRIKGSHNAVKTGMLAAEAAFEALQAGRS
                     ADEL"
     gene            1286..2071
                     /locus_tag="VF686_01850"
     CDS             1286..2071
                     /locus_tag="VF686_01850"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8660580.1"
                     /translation="MTQFPDTAATESLLAFWRDAGVDACFEDAPVDRTHVVPPPAVKA
                     VAKATASVVATPDAGDALSEARRLAAGANTLEELGQAIAAFEGCPLRGMGATQSVFCR
                     GNPAAPLLVIGEGPGAEEDQRGQPFVGKAGQLLDRMLKEAGLEGRVFITNTVFWRPPG
                     NRTPTAAEQAVCAPFVERTFALLKPKAVLLLGAAAAKSVLSTDEGIMRMRGQWRDWRL
                     SEGAVSAPVMPTLHPAFLLRQPMAKRQAWADILSLAARLDADG"
BASE COUNT          336 a          742 c          789 g          389 t
ORIGIN      
        1 cagctcgtcg gctgaccgcc ccgcctgcag cgcctcgaac gcggcttcgg cggccagcat
       61 tccggtcttc accgcgttgt ggctgccctt gatgcgcggc acgttcacga agccggcgct
      121 gtcgccgatc agcactccgc cggggaaggt cagcttcggc accgactgga agccgccctc
      181 ggtaatggcg cgggcgccgt aggagatgcg ggttccgccc tccagatgct cggcgatcga
      241 ggggtgatgc ttgaagcgct ggaactcgtc gaacggcgac aggaaggggt tcttgtagtt
      301 caggtgcacg acgtaaccga tggccacgta gcggtcgccg aaatggtaca tgaagcttcc
      361 gccgccggtg aattcgtcca gcggccagcc ggtggtgtgt tgcgccagcc cggggcggtg
      421 tttgtcagcc gggacctgcc acagctcctt caggccgatg ccgaatttct gcggctgggc
      481 gtcgtcgcac aggttgtggc gggccatgat ggtcttggcc agcgagccgc gcacgccctc
      541 ggcgatgaag acgtatttgc cgtgcagttc gatgcccggc tggaagtcgc cggtcggctt
      601 gccgtggcgg tcgatgccga aaacgccggc gacgacgccc tttacacggc ccgtcggctc
      661 gtcccagacc acgtggctgg cggccatgcc gggatagacc tcaacgccga gcgcctcggc
      721 ctgttcgccc agccagcggg cgacattgcc gagagaggcg atgtagcagc cgtcgttgtg
      781 catcatcggc ggcagcaggg ccatcggcag ggaggcctgc cccatgggcc ccagcaccag
      841 gaagcggtcc tcggtgacgg gggtttccag cggggctccg cgttccttcc agtcggggaa
      901 gagctcgttc agagcgcgcg ggtcgatgac ggcgccggac agtatgtggc cgccgatctc
      961 ggcgctcttc tccagcacgg cgacggtgat gtccttgccg tctttttccg cccgctgctt
     1021 caggcggatg gccgcggcga gtcccgcggg accgccgccg acgatgacga catcgtactc
     1081 catcacctcg cgctcgaccc cggccagggc ctcggcgggc ggcagcttgt cgatcacggc
     1141 ttcctgccag gcgtttgtcg cgccgccctc gatcgcgtcc tcggccatgc cctgatttcc
     1201 tcgcagtcgg tgtggtcttg gtgttttccg cttatcggaa ggtgacgcaa gggtggtcaa
     1261 ggactatccc tgtgccgacc tcatgatgac gcagttcccc gacaccgccg cgaccgaaag
     1321 cctgctcgcc ttctggcgcg acgcgggggt ggacgcctgt ttcgaggacg ccccggtcga
     1381 ccgcacccac gtcgttccgc caccggcggt gaaggcggtg gcaaaggcca cggcctcggt
     1441 tgtggccaca cctgacgccg gcgatgcgct ctctgaagcg cgccgactgg ccgccggggc
     1501 gaacacgctg gaggaactgg gtcaggccat cgcggccttt gaaggctgcc ccctgagggg
     1561 gatgggcgcc acgcagtccg tgttttgccg cggcaatccg gcggctccac tcctggtcat
     1621 cggcgagggc cccggcgcgg aagaggatca gcggggccag cccttcgtcg gcaaggccgg
     1681 acaactgctg gaccggatgc tgaaggaagc cgggctcgag gggcgggtgt tcatcaccaa
     1741 cacggtcttc tggcggccgc ccggcaaccg cacccccacc gcggcggaac aggctgtctg
     1801 cgcaccattc gtcgagcgaa ctttcgccct gctgaagccg aaagccgtgc tgctgcttgg
     1861 cgcggcggct gcaaagtcgg tgctgtcaac cgacgagggc atcatgcgga tgcggggcca
     1921 gtggcgggac tggcggctgt ccgagggcgc ggtttcagcc ccggtgatgc cgacgctgca
     1981 tccggccttc ctgctgaggc agccgatggc caagcggcag gcgtgggcgg acatcctgtc
     2041 gctggcggcg cggctggacg ccgacggctg agctcgttca tccttcagac tcaaaactgc
     2101 cccaaaatgg ggagaaatct tgacacgggc cgggggcgat ggcgccttcc gcgtctgacg
     2161 accgaatgaa ctgcgcagcg tgacctgagt cgagacgggt cactggacgc ttcaaggggg
     2221 ccgcctcagt gctgtttgat ttccgccgcg cgatga
//
LOCUS       DASVVN010000121         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_399430,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000121 DASVVN010000000
VERSION     DASVVN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399430"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF686_01855"
     CDS             <1..312
                     /locus_tag="VF686_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX8660581.1"
                     /translation="SGGARGPDVVLGDRHGSSCEAELTRRLRRAFEALGWRVALNQPY
                     AGGWTTQAWGRPDEGFHAIQIELNRALYFDEAARTPSAGWSRTQKGVARVIAGLLAGE
                     T"
     gene            complement(559..1242)
                     /locus_tag="VF686_01860"
     CDS             complement(559..1242)
                     /locus_tag="VF686_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HEX8660582.1"
                     /translation="MTLFDWLALAFFLVCWLGYGPLLGLLARRSGTLNDDMLIVRRVW
                     MTAMTHREIRLVDSNLLGHTINSGGFFASTNLLLIAAVASVLFGGEQAIEGFASVGAG
                     DVPTRLLEAKLALVLLCLARGLLDFIWSIRQLNYSLALIGAAPEVHTEADRVALGDAA
                     ANLLNPALSAFSQGVRGYYFALAAAAWLFGPAWLALGVAAISCLLIWRQAGSPAARAI
                     RSARRLLEP"
     gene            1413..>1525
                     /locus_tag="VF686_01865"
     CDS             1413..>1525
                     /locus_tag="VF686_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEX8660583.1"
                     /translation="MNIDAITVGPNPPWDVNVIIEIPQGGLPVKYEMDKASG"
BASE COUNT          292 a          511 c          514 g          208 t
ORIGIN      
        1 tcgggcggcg cgcgcgggcc cgacgtggtg ctgggcgacc ggcacggctc gtcctgcgag
       61 gcggagctga cgcggcggct gcggcgggcg ttcgaggcgc tgggctggcg ggtggcgctg
      121 aaccagccct atgcaggcgg atggaccacc caggcatggg gccgacccga cgagggtttc
      181 cacgccatcc agatcgaact gaaccgcgcc ctgtatttcg acgaggccgc gcggaccccc
      241 tccgccggct ggtcgcgcac gcaaaagggc gtcgcgcggg tcatcgcggg cctgctggcc
      301 ggcgagacat aaaaaaagcc gcgcccgaag gcgcggcata aaagtcttta gggaggaaac
      361 gcccaggaag ggcatgacgc ccggaggcgt cgaaaatcaa gttagtgtgc ggtgcacaaa
      421 ccgtcaagac agtatttcgc acctgcgttc acgcaatgta acggcacggt cgttccctgt
      481 gatcgccctg taaccggttt ccggggcaaa aactgtccgg gcgccagcgc atctgcgaac
      541 agccttggcc gttccggctc agggctcgag cagacgccgg gcgctgcgga tggcccgggc
      601 ggccggagac ccggcctgac gccagatcag caggcaggag atggccgcca cgcccagcgc
      661 cagccacgcc ggcccgaaca gccaggccgc tgcggccaag gcgaaatagt agccgcgcac
      721 gccttggctg aaggcgctca gcgccggatt cagcagattg gccgccgcgt ccccgagggc
      781 cacgcgatcc gcctcggtat gcacctccgg ggctgcgccg atcagggcga gcgaatagtt
      841 cagctggcgg atcgaccaga tgaaatccag caggccacgc gccagacaga gcagcaccag
      901 cgccagtttg gcctccagca gccgggtcgg cacatccccc gcccccacgg acgcgaaacc
      961 ctcgatcgcc tgctctcctc cgaacagaac gctggccacg gccgcgatca gcagcaggtt
     1021 ggtcgaggcg aagaagccgc cggaattgat ggtgtggccc agcaggttgc tgtcgaccag
     1081 gcggatctcg cgatgggtca tcgccgtcat ccagacgcgg cggacgatca gcatgtcgtc
     1141 gttcagcgtg ccgctgcgcc gcgccagaag gcccagaagg gggccatagc ccagccagca
     1201 gacgaggaag aaggcgaggg ccagccagtc gaaaagggtc atgggtcgct cctcgtcaag
     1261 gggtcatggt gacgtgcgca ggtctcgcgc tcaagtcagg cccgaccatg tcgcgagcat
     1321 ggcgcaaaac catctccgcc ttgcttcggg cgccgtgcgc gcctatcacg cccgccactt
     1381 tcgtatcgca ctgcacaata atcgagcgcg ccatgaacat cgacgccatc accgtcggcc
     1441 ccaacccgcc ctgggacgtc aatgtcatca tcgagatccc gcaaggcggt ctgccggtga
     1501 agtacgagat ggacaaggcc tcggg
//
LOCUS       DASVVN010000122         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_57038, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000122 DASVVN010000000
VERSION     DASVVN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57038"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF686_01870"
     CDS             complement(<1..870)
                     /locus_tag="VF686_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8660584.1"
                     /translation="MRALTQGFLFFTYLFLAMTAGAFLWSSNLGAGAGVAAGFGVLGL
                     FLAAHTLITAKADKAALKKEIDSVREAHRLLADAMESTQGALTELAQAIESGAVTSNE
                     ALSGEVRMLETLISQMSESLDHRMAQPAPASAFEARHRDQANVLLATIHDALAENRVD
                     LYLQPVVSLPQRRTIFYESFTRLRAADDRVMMPSEYLSVAEGEGLVPAIDNLLLFRCA
                     QIVRRLARQDRKVGVFCNVALSSLGDETFFPQFLDFLSENRDLNQALIFELGQAVFDA
                     RGAVEARNMAKLAD"
     gene            979..>1692
                     /locus_tag="VF686_01875"
     CDS             979..>1692
                     /locus_tag="VF686_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8660585.1"
                     /translation="MELFDLTGKVAVITGSSKGIGKAIAERMAEHGARVVISSRKAGP
                     CQEVADAINAKHGEGRAMVVPANIASKEDLQRLVDETNAAFGQIDILVCNAATNPYAG
                     SMAGINDEQFGKILQNNVVSNHWLIQMVAPQMLERRDGSVIIVSSIGGLRGNALIGAY
                     NISKAADMQLARNLAVEWGPSNVRVNTIAPGLVQTDFAKYLWENPELLKQVTDPAPLK
                     RIGQPDEIAGAAVYLAAPAS"
BASE COUNT          296 a          498 c          631 g          267 t
ORIGIN      
        1 gtcggccagc ttggccatgt tgcgggcctc gacggcgccg cgggcgtcga agacggcctg
       61 gcccagctcg aagatgagcg cctggttgag gtcgcggttc tcggacagga agtcgaggaa
      121 ctgcgggaag aaggtctcgt cgccgagcga ggacagggcg acgttgcaga agacgccgac
      181 cttgcggtcc tggcgggcga gacggcggac gatctgggcg cagcggaaca gcagcaggtt
      241 gtcgatggcg gggaccagac cctcgccctc ggcgaccgac agatattcgg acggcatcat
      301 cacccggtcg tctgcggcgc gcaggcgggt gaagctttcg tagaagatgg tgcggcgctg
      361 gggcagggag acaaccggct ggaggtagag gtcgacgcgg ttctcggcca gggcgtcgtg
      421 gatggtggcc agcaggacat tggcctgatc gcggtgccgg gcctcgaagg cgctggcggg
      481 ggcgggctgg gccatgcggt ggtccagcga ctcgctcatc tgcgagatga gggtctcgag
      541 catgcggact tcgccggaca gggcctcgtt ggaggtgacg gcgcccgact cgatggcctg
      601 ggccagttcg gtgagggcgc cctgcgtcga ttccatggcg tcggcgagca ggcggtgggc
      661 ctcgcggacg ctgtcgattt cctttttcag cgcggccttg tcggccttgg ccgtgatcag
      721 ggtgtgggcg gccagaaaca ggccgagcac gccaaagccc gcggccacgc cggcgccggc
      781 gccgagattg gagctccaca gaaacgcccc ggccgtcatg gccaggaaca gataggtgaa
      841 gaacaggaag ccctgggtga gtgcgcgcat tggaaacgga tcgtccgtct tcccggatgg
      901 aatcgcccga gtatcggggt aacagtgcgg atcgcctata ccggcgcacg gtttctcccc
      961 acttcttcga ggttaacgat ggaattgttc gatctgaccg gcaaggtcgc ggtgatcacc
     1021 ggttcctcga aggggatcgg caaggctatc gccgagcgga tggccgagca cggcgcccgg
     1081 gtggtgatca gttcgcgcaa ggccgggccc tgtcaggagg tcgccgatgc gatcaatgcg
     1141 aagcacggcg agggccgggc catggtcgtg ccggccaaca tcgcctcgaa ggaggacctg
     1201 cagcggctgg tcgacgagac caacgccgcc ttcgggcaga tcgacatcct ggtctgcaac
     1261 gcggcgacca atccctatgc ggggtcgatg gcggggatca acgacgagca gttcggcaag
     1321 atcctccaga acaacgtcgt gtccaaccac tggctgatcc agatggtcgc gccgcagatg
     1381 ctggagcggc gcgacgggtc ggtgatcatc gtctcgtcga tcggaggctt gcgaggcaat
     1441 gcactgatcg gcgcctacaa catctccaag gcggcggaca tgcagctggc gcgcaatctg
     1501 gcggtcgagt gggggccgtc gaacgtgcgg gtcaacacca tcgcgccggg tctggtgcag
     1561 accgacttcg ccaaatacct gtgggagaac cccgagctgc tgaagcaggt gaccgatccc
     1621 gcgccgctga agcggattgg ccagccggac gagatcgcgg gggcggccgt ctatctggcg
     1681 gcgccggcct cg
//
LOCUS       DASVVN010000123         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_267311,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000123 DASVVN010000000
VERSION     DASVVN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267311"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..551)
                     /locus_tag="VF686_01880"
     CDS             complement(60..551)
                     /locus_tag="VF686_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660586.1"
                     /translation="MIRTTFALPAALLLVAATSAAAQTPPPGAAGMGGPMTLEAMQAR
                     QDQMFTRLDANADDVITGAELAPLTQPPMSQMGGPRIRSMITRADADRNARISREEMR
                     SAAAALFTSLDADHDGLVSPTERAAMMPAPPAPPAARMSVPSTAPTDTGGMPAGMEPP
                     NGG"
     gene            667..1629
                     /locus_tag="VF686_01885"
     CDS             667..1629
                     /locus_tag="VF686_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8660587.1"
                     /translation="MSLTPDQTIEALRAAGEPTRLRVLSLLAGEELSVMELSRILEQS
                     QPRVSRHLKLMADAGLIERFPDGARVFYRLSSDPLARRLVDTVLDLLEDAAHEPDERR
                     LEDVRRDRGAAASAYFERVAPQWDRIRSLYVCETAVEAAIKKAAGEGPFERVVDLGTG
                     SGRMLTLLGKTAKMSVGLDLSQNMLNIARANVTRAGLDKVELRHGDIFATRLPEHSAD
                     LVLVHQVLHYLADPAAAVAEAARLVMPGGRLLIVDFAPHQLEHLREEHQHRRLGFADA
                     EMKRWLTGAGLKPSAPIALPPDTDGLTVSIWTAGRPAAAKAKVA"
     gene            1626..2594
                     /gene="metF"
                     /locus_tag="VF686_01890"
     CDS             1626..2594
                     /gene="metF"
                     /locus_tag="VF686_01890"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX8660588.1"
                     /translation="MSISPRSLAEARALLLSPLGPIARAGQDHSRPRVSFEFFPPKSD
                     EAEANLWKAVTRLAPLEPEFVSVTYGAGGSTRERTHRTVERILAETALTPAAHLTCVE
                     ASREDVDAVIEGYRDAGVRHIVALRGDPPGGHSSLGGIGGAYVPRADGYANATELTAA
                     ITAKGGFDVIVGCYPEGHPESPSTAHDIEVLKAKVDAGATRAISQFFFDIDAFLRFRD
                     RVRAAGITIPMSPGIMPVTNFNGLKRMSAACGAAVPDWLAAHFDGLDDDPETRKLIAA
                     SVAAETCARLQEEGFADFHFYTLNRADLVYAICRVLGVRETKAAIV"
     gene            2591..>3545
                     /locus_tag="VF686_01895"
     CDS             2591..>3545
                     /locus_tag="VF686_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX8660589.1"
                     /translation="MSTPLPRSERIAALHRAAKERILVLDGAWGVMIQRGDLSEEDFR
                     GERFAGHNGQMKGNNDILCITRPDVIASLHDQYFAAGADISETNTFSATVIAQDDYKL
                     DEQSVWDINLEGAKLARAAADRWTAKEPHKPRFAAGSIGPLNKMLSMSSDVNDPGARL
                     VTFDQVYDAYRHQVKALNEGGVDLYLIETITDTLNCKAAIKAIKDLEDEGMEALPIWI
                     SGTITDRSGRTLSGQTAEAFWNSVRHARPFAVGFNCALGADLMRPFIAELSRVADTLV
                     AAYPNAGLPNAMGQYDEEPHQTAHFIEEWAASGLVNIVGGCC"
BASE COUNT          565 a         1218 c         1200 g          562 t
ORIGIN      
        1 tggcggcgct tcaggacgtc tgctgatcgg atcgcgaggc gtcgcctgta accacggttc
       61 tagccgccgt tcgggggttc catgccggcg ggcatgccgc ccgtgtcggt tggagcggtg
      121 gagggcaccg acatgcgcgc ggcgggcggg gccggcggag cgggcatcat ggcggcgcgc
      181 tcggtcggac tgacgagacc gtcgtgatcc gcatccaggc tggtgaacag ggcggcggcg
      241 gcgctgcgca tctcctcgcg gctgatgcgc gcgttccggt cggcgtcggc gcgggtgatc
      301 atcgagcgta tgcgcggtcc gcccatctgg ctcatcggcg gctgggtcag gggcgccagc
      361 tcagcgccgg tgatcacgtc gtcggcgttg gcgtcgagcc gggtgaacat ctggtcctgc
      421 cgcgcctgca tggcctccag ggtcatgggc ccgcccatgc ctgcggcgcc gggcggcggc
      481 gtctgggcgg cggcgctcgt cgcggcgaca agcaggaggg cggctggaag ggcgaaggtg
      541 gtgcggatca tggcgtatct cctgccgggt tccactggac cagacgagtg tacgcccgtt
      601 tcagcaagct tgtcgcgcgt ctgaattcgc ttgcaaaatc atataaggat atctttatat
      661 aaacacatgt cgttgacccc cgaccaaacc atcgaagccc tgcgcgccgc gggcgagccg
      721 acccgtctca gggtgctgtc gctgctggcc ggcgaggaac tgtcggtcat ggagctgtcg
      781 cggattctgg aacagagcca gccgcgcgtg tcacggcatc tgaagctgat ggcggacgcg
      841 ggtctgatcg aacggttccc ggacggggcg cgcgtcttct atcggctgtc gtcggatccg
      901 ctggcgcgac ggctggtcga caccgtgctg gatctgctgg aggacgccgc tcacgagccg
      961 gacgagcgcc ggctggaaga cgtgcgccgc gaccgcggag ccgccgccag cgcctatttc
     1021 gaacgcgtcg cgcctcagtg ggaccggatc cggtcgctgt acgtctgcga aaccgccgtc
     1081 gaggccgcga tcaagaaggc cgccggtgaa ggaccgttcg aacgcgtggt cgatctgggc
     1141 acgggctcgg gccggatgct gaccctgctg ggcaagacgg cgaagatgtc ggtcgggctg
     1201 gatctgagcc agaacatgct caacatcgcc cgcgccaacg tcaccagggc cgggctggac
     1261 aaggtcgagc tgcgtcacgg cgacatcttc gccacccgcc tgcccgaaca cagcgccgat
     1321 ctggtgctgg tgcatcaggt cctgcactac ctcgccgatc cggcggccgc cgtggccgag
     1381 gcggcgcgac tggtcatgcc cggcggacgg ctgttgatcg tcgatttcgc cccgcaccag
     1441 ctggagcacc tgcgcgagga acatcagcac cgccgcctcg gattcgccga cgccgagatg
     1501 aagcgctggc tgaccggggc gggcctgaag cccagcgcgc cgatcgccct tccccccgac
     1561 accgacggcc tgaccgtctc gatctggacc gccgggcgtc cagccgcagc caaggccaag
     1621 gtcgcatgag catttccccc cgcagtctcg ccgaggcccg cgccctgttg ctgtcgcctc
     1681 tgggccccat cgcccgcgcc ggccaggacc actcgcggcc gcgtgtctcg ttcgaattct
     1741 tcccgcccaa gtccgacgag gccgaggcca atctgtggaa ggcggtcacg cgtctggccc
     1801 cgctggagcc ggagttcgtc tcggtcacct atggcgccgg cggttcgacg cgggaacgca
     1861 cgcaccggac ggtagagcgc atactcgccg agacggcact cactccggcg gctcacctga
     1921 cctgcgtaga ggcaagccgc gaggatgtgg atgcggtgat cgagggatac cgtgacgccg
     1981 gcgtgcgcca catcgtggcc ctgcggggcg atccgccggg cgggcactcc agtcttgggg
     2041 gcatcggcgg cgcctatgtt ccgcgtgcgg acggttacgc caatgcgacc gaactgaccg
     2101 ccgccatcac ggccaagggc gggtttgacg tcatcgtcgg ctgctatccc gagggccatc
     2161 ccgaaagccc gtctaccgcc cacgacatcg aggtgctgaa ggccaaggtc gacgccggcg
     2221 ccacgcgcgc catcagccag ttcttcttcg acatcgacgc cttcctgcgc ttccgcgacc
     2281 gggtgcgggc ggccgggatc accattccga tgtcgccggg catcatgcct gtgaccaact
     2341 tcaatggttt gaagcggatg tcggcggcgt gcggcgcggc cgtgccggac tggctggcgg
     2401 cccatttcga cgggctggac gacgaccccg agacccgcaa gctgatcgcc gcctcggtcg
     2461 ccgccgagac ctgcgcccgg ctgcaggagg aaggcttcgc cgacttccac ttctacaccc
     2521 tgaaccgcgc cgacctggtc tatgcgatct gccgggtcct tggcgtgcgt gagacgaagg
     2581 ccgccatagt atgagcaccc cccttccccg cagcgaacgc atcgccgccc tgcatcgggc
     2641 cgcgaaggag cgcatcctcg tcctcgacgg cgcctggggc gtgatgatcc agcgcggcga
     2701 cctgtccgaa gaggattttc gcggcgaacg cttcgccggt cacaatggcc agatgaaggg
     2761 caacaacgac atcctgtgca tcacccgccc ggacgtcatc gccagcctgc acgaccagta
     2821 tttcgccgcc ggcgccgaca tcagcgagac caacaccttc tcggccaccg tcatcgccca
     2881 ggatgactac aagctggacg aacagtcggt gtgggacatc aatctggaag gggcgaaact
     2941 ggcccgcgcg gccgccgacc gctggaccgc gaaggagccg cacaagccgc gcttcgccgc
     3001 cggttcgatc ggtccgctga acaagatgct gtcgatgtcg tctgacgtga acgaccccgg
     3061 cgcgcggctg gtgaccttcg accaggtcta tgacgcctac cgccatcagg tgaaagcgct
     3121 gaacgagggc ggcgtcgacc tgtatctgat cgaaaccatc accgacacgc tgaactgcaa
     3181 ggccgcgatc aaggccatca aggacctcga ggacgagggc atggaggccc tgccgatctg
     3241 gatttcgggc accatcaccg accgttcggg ccggaccctg tcgggccaga cggccgaggc
     3301 cttctggaac agcgtgcgcc acgccagacc cttcgccgtc ggtttcaact gcgccctcgg
     3361 cgccgacctg atgcgcccgt tcatcgccga gctgagccgc gtcgccgaca ccctggtcgc
     3421 cgcctatccc aacgccggcc tgcccaacgc catgggccag tacgacgagg agccgcacca
     3481 gaccgcccac ttcatcgagg aatgggcggc ctccggtctg gtcaatatcg tcggcggctg
     3541 ttgcg
//
LOCUS       DASVVN010000124         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_57056, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000124 DASVVN010000000
VERSION     DASVVN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57056"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /gene="alaS"
                     /locus_tag="VF686_01900"
     CDS             complement(<1..1505)
                     /gene="alaS"
                     /locus_tag="VF686_01900"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX8660590.1"
                     /translation="MASLNDIRSSFLDYFAKADHVVTPSAPLVPQNDPTLLFVNAGMV
                     PFKNVFTGAEAPPAPRAASSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KAQAIELAWTLITREYGLPADKLTVTVYATDDEAFDLWRRIAGLPEERIIRIATSDNF
                     WSMGDTGPCGPCSEIFNDHGPGIPGGPPGSDDQDGDRFIEIWNLVFMQFEQFADGSRT
                     PLPKPSIDTGMGLERITAVLQGVHDNYDTDLFRALIDASEALTGVKVDGGRGPSHRVI
                     ADHLRAASFLIADGVTPSNEGRGYVLRRIMRRAMRHAWLLGAQDPLMHRLAPTLAEQM
                     GDAYPELRRAQPMITETLRQEEERFRTTLGRGIGLLEEATANLNAGDQLPGETAFKLY
                     DTYGFPLDLTQDAVRAKGLTVDTSGFEAAMDRQRTMAREAWTGSGAKGVAAEWFAIRD
                     RLGPTVFTGYEGVDATGEVLALVRDGREIDVAETGEEVQVLADQTPFYAESG"
BASE COUNT          222 a          503 c          532 g          256 t
ORIGIN      
        1 ccggattcgg cgtagaaggg cgtctggtcg gcgagcacct gcacttcttc gccggtttcg
       61 gcgacgtcga tctcacgtcc gtctctgacc agcgccagca cctcgcccgt agcgtcgacc
      121 ccctcgtagc cggtgaacac cgtcggtccc agacgatcgc ggatggcgaa ccattcggcc
      181 gccacgccct tcgcgcccga ccccgtccag gcttcccgag ccatcgtccg ctgccggtcc
      241 atggccgcct cgaagccaga ggtgtcgacg gtcagcccct tggcgcgcac cgcatcctgg
      301 gtcaggtcaa gcggaaagcc gtaggtgtcg taaagcttga atgcggtctc gccaggcagc
      361 tggtcgccgg cgttgaggtt cgccgtcgcc tcctcgagaa ggccgatgcc gcgccccagc
      421 gtggtgcgga agcgctcctc ctcctggcgc agcgtctcgg tgatcatggg ctgcgcgcgg
      481 cgaagttccg gataggcgtc gcccatctgc tcggcgagcg tgggcgcgag gcgatgcatc
      541 agcggatcct gcgcgcccag cagccaggcg tggcgcatcg cgcgccgcat gatccggcgc
      601 agcacgtagc cgcgaccttc gttcgacggc gtcacgccgt ccgcgatcag gaaggaggcg
      661 gcgcgcaggt ggtcggcgat cacccggtgg gagggccccc gaccgccgtc cacctttaca
      721 ccggtaagcg cctcgctggc gtcgatcagg gcgcggaaca ggtcggtgtc gtagttgtcg
      781 tgcacgccct gcagcacggc ggtgatgcgc tcgagcccca tgccggtgtc gatcgacggc
      841 ttgggcagcg gcgtgcgcga gccgtcggcg aactgctcga actgcatgaa caccaggttc
      901 cagatctcga tgaaccggtc gccgtcctgg tcgtccgagc ccggcgggcc gccggggatg
      961 ccgggcccgt ggtcgttgaa gatctccgaa caggggccgc aggggccggt gtcgcccatg
     1021 gaccagaagt tatcggaggt cgcgatgcgg atgatccgct cctccggcag acccgcgatc
     1081 ctgcgccaga ggtcgaaggc ttcgtcgtcc gtggcgtaga cggtgaccgt gagcttgtcg
     1141 gccggcaggc cgtactccct ggtgatcagc gtccacgcca gctcgatcgc ctgcgccttg
     1201 aagtagtcgc cgaaggagaa attccccagc atctcgaaga aggtgtggtg gcgggcggtg
     1261 tagccgacgt tgtccaggtc gttgtgcttg ccgccggcgc ggacgcattt ttgcgagctg
     1321 gccgcgcgcg gcgcgggcgg cgcttccgcg cccgtgaaca cgttcttgaa cggcaccatg
     1381 ccggcgttga cgaacaacag ggtcggatcg ttctgcggca ccagcggcgc cgagggcgtg
     1441 accacgtgat ccgccttggc gaagtagtcg aggaagctgc tgcggatgtc gttcaggctg
     1501 gccatggagc ggt
//
LOCUS       DASVVN010000125         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_214953,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000125 DASVVN010000000
VERSION     DASVVN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214953"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VF686_01905"
     CDS             <1..577
                     /locus_tag="VF686_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8660591.1"
                     /translation="KEMSDQSYADMYKANFFSLGHDRITRFRKPIIAAVNGFALGGGC
                     ELAMLCDFIVASEKAKFGQPEINLGVAPGIGGSQRLTRLVGKSKAMDMVLTARMMDAA
                     EAERAGLASRVVPHETLLDETRKIAAKIAAQSPLAVMANKEMVNAALETTLTQGVQFE
                     RRLFHSLFAFEDQKEGMAAFVEKRKPNFTGR"
     gene            684..953
                     /gene="rpsT"
                     /locus_tag="VF686_01910"
     CDS             684..953
                     /gene="rpsT"
                     /locus_tag="VF686_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX8660592.1"
                     /translation="MANNAGAKKAIRKIAARTEVNKARRTRVRTFLRKFQEAVTGGDA
                     GAAKTAFVEAQSELMRAVSKGVVHKNTGSRKVGRLAAQLKKMSAA"
     gene            1416..>1942
                     /locus_tag="VF686_01915"
     CDS             1416..>1942
                     /locus_tag="VF686_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX8660593.1"
                     /translation="MAGMTDPDRIWTEAAGRLRTEIGEGPFSSYIAPSAVRADGSGQL
                     ILVTPTAYARDWVRKNALRRMNELWLGLDGLSRRLDVRCRAEMGSTPSASQVAAGRAE
                     GAAILTLPQASPAPMPMATVPPVADGARAVRAAGLQERLTFDSFVEGQGNAFALAIAK
                     QAAAWADGHFNPIFF"
BASE COUNT          329 a          679 c          611 g          323 t
ORIGIN      
        1 caaggagatg tccgaccagt cctacgccga catgtacaag gccaacttct tctcgctggg
       61 ccacgaccgg atcacccgct tccgcaagcc gatcatcgcc gcggtcaacg gcttcgcctt
      121 gggcggcggc tgcgagctgg ccatgctgtg cgacttcatc gtggcgtccg agaaggcgaa
      181 attcggccag cccgagatca acctcggcgt cgcccccggc atcggcggct cccagcgcct
      241 gacccgtctg gtcggcaagt ccaaggccat ggacatggtc ctgaccgccc gcatgatgga
      301 cgccgccgag gccgaacgcg ccggcctcgc cagccgcgtc gtcccgcacg agacccttct
      361 ggacgagacc cgcaagatcg ccgcgaagat cgcggcccag agcccgctgg ccgtcatggc
      421 caacaaggaa atggtcaacg ccgcgctcga gaccaccctg acccaaggcg tccagttcga
      481 acgccgcctg ttccacagcc tgttcgcctt cgaggaccag aaagagggga tggcggcctt
      541 cgtggagaag cggaagccga acttcaccgg ccgctgagac tcgccttgat cgcgcccacc
      601 ctttccgcta tagccgcgcg ctctcgaaga tttccccgcc gtggaccgtg ctccgcggcg
      661 tttccgttcg taaggtttga ctgatggcca acaatgccgg cgccaagaaa gcgatccgca
      721 agatcgctgc ccgcaccgaa gtgaacaagg cgcgccgcac gcgcgtccgc accttcctgc
      781 gcaagttcca ggaagccgtc accggcggcg acgccggcgc tgcgaagacc gcgttcgtcg
      841 aggctcagtc cgagctgatg cgcgccgtct ccaagggcgt ggtccacaag aacacgggct
      901 cgcgcaaggt cggccgtctg gccgcccagc tgaagaaaat gtccgctgcc tgacacggcg
      961 gcgttctgac ggcctttcat cggcctgtca cagttaaatc cagcaaattc aacggcgagg
     1021 gcgctaacgc tccctcgccg tttgcacgtg tgcggctaac cgttcagcca ggaatcggtg
     1081 ctcccgtctg tggcgaaatg gggtttttcg ttgactgcgt ttggctttag cggagtcaac
     1141 aattttaagt gcaaatcgag tcgcgaatca gcgttgacgc ccccctcgcc gggcgtaagt
     1201 ttggggcgct gacgcacttc catccccaca cggatgaaga cggcgcggaa ccagttttcg
     1261 tgtcggcgtg agacgcctgt tcaagagttc gaccccaccc gcgcggcgcc atcgccacgc
     1321 gggacaggag aagtcgtccc gttttcaggc gcgctcggaa ggacgtcggc ggtgatggat
     1381 caaacgggtg gctgatgttt acggggagcg cgggaatggc ggggatgacg gatccggatc
     1441 gtatctggac cgaggcggcg ggccgtcttc gcactgaaat cggcgagggg cccttcagct
     1501 cctacatcgc cccgtcggcc gttcgcgccg atggttcggg ccagctgatc ctggtcaccc
     1561 cgaccgccta tgcccgcgac tgggtgcgca agaacgccct gcgccgcatg aacgagctgt
     1621 ggctgggcct ggacggcctg tcgcgccgtc tggacgtccg ctgccgcgcc gaaatgggct
     1681 cgaccccctc ggcctcgcag gtcgccgccg gccgcgctga gggcgccgcc atcctgaccc
     1741 tgccgcaggc ctctcccgct ccgatgccga tggccaccgt cccgccagtc gccgacgggg
     1801 cccgtgcggt ccgcgccgcc ggcctgcagg agcggctgac cttcgacagc ttcgtcgagg
     1861 gacagggcaa cgccttcgcc ctcgccatcg ccaaacaggc cgcagcctgg gccgacggcc
     1921 acttcaatcc gatcttcttc tg
//
LOCUS       DASVVN010000126         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_4352, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000126 DASVVN010000000
VERSION     DASVVN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4352"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VF686_01920"
     CDS             complement(<1..1203)
                     /locus_tag="VF686_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8660594.1"
                     /translation="MTTPAWNETDRLAALHSYDILDSEREPAFDDITALVARLCDAPI
                     AVVNLIDADRQWFKSEVGLGVRETPLETSFCRHALLEQDRLVVPDATQDPRFEGNPLV
                     TGEAGLRFYAGVLLKDQNGLPLGTLCVLDTRARPEGLNATQAEALEVLARQVMNLLNL
                     RKAIAAGRRADDDLIAAAAALSESEAKFRAIADSMPQMVWSTRPDGFHDYYNARWYEF
                     TGVPAGSTDGEGWNGMFHPDDQERAWERWSHCLKTGEPYEIEYRLRRADGVYRWTLGR
                     AQPIRNEQGEITRWFGTCTDIEDLKRIVDSKDLLSQELSHRIKNIFAVVSALIALSAR
                     QFPEAKDFAAAVRTRINALARAHEFVRPHTDTSRPTVGTMTLHAFLNDLFKPYATLDG
                     RTRVTVSGD"
     gene            complement(1293..>1813)
                     /locus_tag="VF686_01925"
     CDS             complement(1293..>1813)
                     /locus_tag="VF686_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8660595.1"
                     /translation="AKRVAERALAGHVAAAVGTATHYHADYVHPWWSPTLNKLTQIGA
                     HIFYRWKGIYGEPAAFSQRYSGREPLIDEARFSRPRIQIATAAEAEKALAEAAANGEG
                     AMRTVEINGQTRVVGVASLGGRRQATPAEIADINQRLSAFEAPAAATPAPVVVAPGVT
                     PMDVEEVGRPGS"
BASE COUNT          276 a          601 c          631 g          305 t
ORIGIN      
        1 gtcgccgctg accgtgaccc gcgtccgccc gtccagggtc gcatagggtt tgaagagatc
       61 gttgaggaag gcgtgcagcg tcatcgtgcc gacggtgggg cgcgaggtgt cggtgtgcgg
      121 ccggacgaat tcgtgcgcgc gggccagggc gttgatccgg gtccgcacgg cggcggcgaa
      181 gtccttggcc tcggggaact ggcgcgccga cagggcgatc agggccgaga cgacggcgaa
      241 gatgttcttg atccgatggc tgagttcctg gctcagcaaa tccttggaat cgacgatccg
      301 cttgaggtcc tcgatgtccg tacaggtgcc gaaccagcgg gtaatctccc cctgttcgtt
      361 ccggatcggc tgagcccgcc ccagcgtcca gcgatagacg ccgtcggcgc ggcgaagccg
      421 gtactcgatc tcatagggct cgcccgtttt caggcaatgg ctccagcgtt cccaggcccg
      481 ctcctgatcg tccggatgga acatcccgtt ccagccctcg ccgtcggtcg accccgccgg
      541 cacgccggtg aactcgtacc agcgggcgtt atagtagtcg tggaagccgt ccggccgggt
      601 cgaccagacc atctgcggca tggagtcggc gatggcccgg aatttcgctt cgctctcgga
      661 cagggcggcg gcggcggcga tcagatcgtc gtcggcgcgt cgtcccgccg cgatcgcctt
      721 gcgcaggttg agcaggttca tcacctgccg cgccagaacc tccagcgcct ctgcctgggt
      781 cgcgttcagc ccctcgggac gggcgcgggt gtccagcacg cacaaagtgc ccagcggcag
      841 gccgttctga tccttgagca ggacgccggc gtagaacctc agacccgcct ccccggtgac
      901 cagtggattg ccctcgaacc ggggatcctg cgtggcgtca ggtacgacca gccggtcctg
      961 ctccagcagg gcgtgacggc agaacgaggt ttccagcggg gtttcccgca cgcccagacc
     1021 gacctcggac ttgaaccact gccggtcggc gtcgatcaga ttgactacgg cgatcggcgc
     1081 gtcgcacagc cgcgccacca gcgccgtgat gtcgtcgaac gcaggctccc gctcggagtc
     1141 cagaatatcg taggaatgca gggccgcgag gcgatccgtc tcgttccagg cgggcgtggt
     1201 cattcagggt ccgtatctcg cgccaaggcg cggccgacat cacctaatgc ctaaaaggcg
     1261 atgccgttgc aaagcttccg atctgcggga aatcaggagc cgggacggcc cacttcctcg
     1321 acgtccatcg gggtcacccc gggcgcgacg accaccggtg cgggagtcgc ggcggcgggc
     1381 gcctcgaagg cggaaagacg ctggttgatg tcggcgatct cagcgggggt ggcctgacgg
     1441 cggccgccca gcgaggcgac gcccacaacg cgggtctggc cgttgatctc gaccgtccgc
     1501 atcgcgcctt cgccgttggc ggcggcctcg gccagcgcct tttcggcctc tgccgccgtg
     1561 gcgatctgga tgcgggggcg cgagaagcgg gcctcgtcga tcaggggctc acggcccgaa
     1621 tagcgctggc tgaaggcggc cggttctccg tagatcccct tccaccgata gaagatgtgg
     1681 gcgccgatct gggtcagctt gttcagcgtc ggcgaccacc acggatgcac gtagtcggcg
     1741 tgatagtggg tcgccgtgcc gacggcggcg gcgacgtgac cggccagcgc cctttcggcc
     1801 acccgtttgg cgc
//
LOCUS       DASVVN010000127         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_4354, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000127 DASVVN010000000
VERSION     DASVVN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4354"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..995
                     /gene="phaP"
                     /locus_tag="VF686_01930"
     CDS             462..995
                     /gene="phaP"
                     /locus_tag="VF686_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274959.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEX8660596.1"
                     /translation="MATQERAARTAKTAANQAADTTTEAAREAAERFQAGAKTFQDAS
                     ERLSTAGNQAFKETVERSLSTLNQLNDVSKKNLEAVVASIGAATRGAEAIGAQAMSYS
                     KSSVEQGVDAARALSAVKSVQEAVELQTNFARTALETYLAEMNRMSETVAASVKESLA
                     PLNERATAAMETLQSTR"
     gene            1136..1546
                     /locus_tag="VF686_01935"
     CDS             1136..1546
                     /locus_tag="VF686_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8660597.1"
                     /translation="MTSETASGRSNLRVLVVEDDPNSRKVLVHALDAMKAELTLAENG
                     AQAVDAFRKAPFDVVLMDLQMPVMDGYAATREIRAHESRGGGGGRTPILVVSAHTRPW
                     EIEAAQSAGADHHLGKPINVPALLGTIEAVTTPL"
BASE COUNT          298 a          561 c          513 g          214 t
ORIGIN      
        1 cgcgctgggc gtacagcacc tcgcccgaat gagcgtcgat cacgatcgcg gcgtacttcg
       61 gctgagtcgc ggggctcgcc tgggcatagg cctgggcggg cagaatcgtc ccggcgcagg
      121 caagcgaaag cactgcggcg aagagggaga tcagaagccg gcgggcgggc tggtacatgc
      181 ctacggtccg tcagtggaat cgcgactcgg cggtgcaagc cgcctgccag gagaatttac
      241 tatgagaatg gttcgttgct ggcgggtaaa cacgacgtga acggccacgt tgtggacggg
      301 gctgcgaccg cctgccgcag tgcgatgcac aaaagtcatg gtgcagtgca caaaaaatcc
      361 ttgacctccg ggtcattttg acccaaatga accctcaggg ccgctccccc aggagaggct
      421 gttaaccacc tccccagtcc gttccaatcg gagacaaccc tatggctact caagagcgcg
      481 cggcccgtac cgcgaagacc gctgccaacc aggcggccga caccaccacc gaagccgccc
      541 gtgaagccgc tgagcgcttc caggccggcg ccaagacctt ccaggacgct tccgagcgcc
      601 tgtcgacggc cggcaaccag gcgttcaagg aaaccgtcga gcgttcgctc tcgaccctga
      661 accagctgaa cgacgtctcg aagaagaacc tcgaagccgt cgtggcctcc atcggcgcgg
      721 ccacccgcgg cgctgaagcc atcggcgccc aggcgatgag ctactcgaag tcgtcggtcg
      781 agcagggcgt cgacgccgcc cgcgccctgt ccgccgtgaa gagcgtgcag gaagcggtcg
      841 agctgcagac caacttcgcc cgcaccgcgc tcgagaccta cctggccgag atgaaccgca
      901 tgtcggaaac cgtcgcggcc tccgtgaagg agtcgctggc cccgctcaac gagcgcgcca
      961 cggccgccat ggaaaccctg cagtcgaccc gctaagcgga ccgacgcaga cctgatcgag
     1021 gcccggggcg gcgacgcccc gggccttttt ctttgcctcg cgcggctgtc gggtgcgaaa
     1081 tggcggacgc ggcctcacca ggccgcgtgt gagcttcgag ggatagtccg ccgtcatgac
     1141 gtccgaaacg gccagcggcc gctccaacct gcgcgtgctc gtcgtcgaag acgaccccaa
     1201 cagccgcaag gtcctggtcc acgcgctcga cgccatgaag gccgagctga cgctggcgga
     1261 gaacggcgcg caggcggtgg acgcgttccg caaggcgccg ttcgacgtcg tcctgatgga
     1321 cctgcagatg ccggtgatgg acggctacgc cgccacccgc gagatccgcg cccacgagag
     1381 ccgcggcggc ggcggcggcc gcacccccat cctggtggtc agcgcccaca cccgcccctg
     1441 ggagatcgag gccgcccaga gcgccggcgc cgaccaccac ctcggcaagc caatcaacgt
     1501 ccccgccctg ctgggcacca tcgaagcagt caccacgcca ttgtaaatct ccctcccgcc
     1561 tgtcgggacg gacaggcggg cgcagc
//
LOCUS       DASVVN010000128         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_214999,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000128 DASVVN010000000
VERSION     DASVVN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214999"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VF686_01940"
     CDS             complement(<1..730)
                     /locus_tag="VF686_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8660598.1"
                     /translation="MRSPRRLLLLLAASTTVLIAASPALAQVAPSDPWAQATSDIPAD
                     SNVRFGVLPNGMQYAVLRNATPAGQASLRLRIDAGSLMENENQLGLAHFMEHMAFNGT
                     TNIPENELLRILERLGLAFGADTNASTGFDQTIYMLELPRTNDETVDASLRIMREQVS
                     EALMASDAVDAERGVIEGEERTRNTPQLRVSKAQLGLIAPGQRLSNRFPIGDLNIIRN
                     APRERFVEFYQAYYRPSRATLVAVG"
     gene            complement(872..>1521)
                     /gene="hfaD"
                     /locus_tag="VF686_01945"
     CDS             complement(872..>1521)
                     /gene="hfaD"
                     /locus_tag="VF686_01945"
                     /inference="COORDINATES: protein motif:HMM:NF037936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holdfast anchor protein HfaD"
                     /protein_id="HEX8660599.1"
                     /translation="IPAAGSVTSEAIVNAVAVNSNATSGQNLSITQRSTGDFVEAEAS
                     ANAGNAWDLAARARASGNQAILYNEGGSVVTESDQDNSAFIRASALTTAYDYGQAEAQ
                     ARGVANELSVGNNDVFVEIDNTQFNSGGVDVTATFSGTNGYDVYVGAEAVGNSVTGYA
                     CSECQATLDATNTQTNNGNVSAVANTTVAGSGRAVITGATAVGNSANFYVSRPGN"
BASE COUNT          227 a          463 c          546 g          285 t
ORIGIN      
        1 cgcccacggc gaccagggtc gcgcgagaag ggcggtagta ggcttggtag aattccacga
       61 accgttcgcg cggcgcgttg cggatgatgt tcaggtcgcc gatgggaaag cggttcgaca
      121 gccgctggcc gggagcgatc aggcccagtt gggccttcga gacgcgcagt tgcggcgtgt
      181 tgcgggtccg ctcctcgcct tcgatcacgc cgcgctcggc gtcgacggcg tctgacgcca
      241 tcagggcctc ggacacctgt tcgcgcatga tgcgcaggct ggcgtcgacc gtctcgtcgt
      301 tcgtgcgcgg cagctccagc atatagatgg tctgatcgaa gcccgtggag gcgttggtgt
      361 cggcgccgaa cgccaggccc agccgctcga gaatgcgcag cagctcgttc tccgggatgt
      421 tggtcgtccc gttgaaggcc atgtgctcca tgaagtgggc caggccgagc tggttctcgt
      481 tctccatcag ggaaccggcg tcgatgcgca gacgcagcga ggcctgaccg gccggcgtgg
      541 cgttgcgcag cacggcgtac tgcatgccgt tgggcaggac gccgaaacgg acgttggaat
      601 cagcgggaat gtcgctggtc gcttgggccc acggatccga cggcgcgacc tgcgccagcg
      661 ccggcgacgc cgcgatcagg acagtggtgg aggccgcgag gagcagcagc aggcgacggg
      721 gggaacgcat taatcggttt ctttccgaag ggcgcgatcg ctcccggatc ggggccgcgc
      781 cggaggttgg gcaggatccg ccgacgtctg ccgtcgccgt ccccgttgca acctttcagt
      841 gaccgatgac tgtcactcaa gttaccgaag attaatttcc cggcctcgag acgtaaaagt
      901 tggccgagtt gcccaccgcg gtcgccccgg tgatgaccgc gcggccggac cccgcgactg
      961 tggtattggc cacggcggaa acgttgccgt tgttggtctg ggtgttggtt gcatccagcg
     1021 tggcctggca ttccgagcag gcgtagccgg tgacgctgtt gccgacggct tccgcgccga
     1081 catagacgtc atagccgttc gtgcccgaga aggtggccgt gacgtccacc ccgcccgagt
     1141 tgaactgggt gttgtcgatc tcgacgaaga cgtcgttgtt tccgaccgac agctcattgg
     1201 cgacgccgcg cgcctgtgct tccgcctggc catagtcata ggcggtggtc agggcggatg
     1261 cccggatgaa ggcgctgttg tcctgatccg actcagtcac gaccgatcca ccttcattat
     1321 agaggatggc ctggttgccg ctggcgcggg cgcgggctgc caggtcccag gcgttgccgg
     1381 cgttggcgct ggcctcggct tcgacgaagt cgccggtcga gcgctgggtg atcgacaggt
     1441 tctggcccga ggtggcgttg ctgttgaccg cgacggcgtt gacgatggcc tcgctggtca
     1501 cgctgccggc ggcgggaatg t
//
LOCUS       DASVVN010000129         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_425335,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000129 DASVVN010000000
VERSION     DASVVN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425335"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF686_01950"
     CDS             <1..300
                     /locus_tag="VF686_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8660600.1"
                     /translation="RTDHHLRTLGLGPVKVSIADTKSRWGSCSPHNRSIRYSWRVIMA
                     PPAVADYLAAHEVAHLVHADHSPAYWSVVQRLVGDHRPFRKWLRENGPALHAVGR"
     gene            complement(528..>2167)
                     /locus_tag="VF686_01955"
     CDS             complement(528..>2167)
                     /locus_tag="VF686_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8660601.1"
                     /translation="LKRKVQELMLAVWLEMKFSKKEILALYLNRVYFGAGAYGIEAAS
                     QRYFDKSAKQLTVGEAALLAGLLKAPSRYSPVSESERAGVRATVVLNEMVDAGVITPE
                     QRAEAAASPVRVSRTLSSQHAQYFVDWLDKQIRELVGEPTEDMIVETTLDLTLQTQAE
                     RAVHRILVRDKSKGVQQAALVALDGDGRVRAMIGGASYADSQFNRATDARRQAGSAFK
                     PFVYLTALESGYTPETRVNDAPIRIGNWSPRNYSGTFAGEMSLSQAVAQSVNTVAAGL
                     ADQIGRDNVARTARRLGITSRIGLEPAMALGAVEVSPIEMAQAYDAFANGGKRVRAYG
                     ISRIRTPQGRVIYQRSARADGGSVQAISNPFLYYQNQMLRGVMTGGTGRSAAIAGRDV
                     AGKTGTTSDYKDAWFVGYTGGFVTAVWVGKDDNTPMRGVTGGSSPAAIWRGFMEAALP
                     RLNAPAIPNGPAMPEGWQPPNPVENLIGEIGDLFGDSPPAEEEIPPEDQPTLEPRRPE
                     PGPPVVRPTAPQPQRDPPDQRPEPAAPPEKRADPLFF"
BASE COUNT          310 a          702 c          798 g          357 t
ORIGIN      
        1 cgcaccgacc accatctgag aaccctgggc ctgggcccgg tgaaggtgtc gatcgccgac
       61 accaaatccc gctggggctc gtgcagcccg cacaaccgtt cgatccgcta cagctggcgc
      121 gtgatcatgg ctccacccgc cgtggccgac tatctggccg cccacgaggt ggcgcatctg
      181 gtccatgcgg accacagccc ggcctactgg tcggtggtgc agcggctggt cggcgaccac
      241 cggccgttcc ggaagtggct gcgggaaaac gggcccgcgc tgcacgcggt ggggcggtag
      301 gttgtcatcc cggaagctgc gcagcagcta tccgggaccg catacgctta ttgatcaaaa
      361 gctcccggaa atcgcatcgc gattgtccgg gaaacaggca accgtcgcgc ggggtcctga
      421 cccgtctccc ggatagctgc tggcgcagct tccgggaggg tcaagtttat gcaggcaagc
      481 gtcccggatc accgcttcgc ggcgtccggg atgacaagcg ccataactca gaagaacagc
      541 ggatccgctc tcttctccgg cggcgcggcc ggttccgggc gctggtccgg cggatcgcgc
      601 tggggctggg gcgcggtcgg gcggacgacg ggcgggcccg gctccggccg gcgcggctcc
      661 agcgtcggct ggtcctcggg cgggatctct tcctcggcgg gcgggctgtc gccgaacagg
      721 tcgccgatct cgccgatcag gttctcgacc ggattgggcg gctgccagcc ctcgggcatg
      781 gcggggccgt tggggatggc cggggcgttc aggcgcggca gggccgcctc catgaagccg
      841 cgccagatgg cggccgggga cgagccgccg gtgacgccgc gcatcggggt gttgtcgtcc
      901 ttgccgaccc agaccgcggt gacgaagccg ccggtgtagc cgacgaacca ggcgtccttg
      961 tagtccgagg tcgtgccggt cttgcccgcc acgtcacggc cggcaatggc cgcggaccgg
     1021 cccgtgccgc cggtcatgac cccgcgcagc atttggttct ggtagtagag gaaggggttg
     1081 gagatggcct ggaccgagcc gccgtcggca cgcgccgagc gctggtagat cactcgcccc
     1141 tgcggcgtgc ggatgcggct gatgccgtag gcgcggacgc gtttgccgcc gttggcgaag
     1201 gcgtcatagg cctgggccat ctcgatcggc gagacctcga cggcgcccag ggccatggcg
     1261 ggctcaagcc cgatgcggct ggtgatgccg aggcggcggg cggtgcgggc gacgttgtcg
     1321 cggccgatct ggtcggcgag gccggcggcg acggtgttca ccgactgggc cacggcctgc
     1381 gacagggaca tctcgcctgc gaaggtcccc gaatagttgc gcggcgacca gttgccgatg
     1441 cggatcgggg cgtcgttgac ccgcgtctcg ggcgtgtagc cactttccag tgcggtcaga
     1501 tagacgaagg gcttgaaggc tgaaccggcc tgacggcgag cgtcggtggc gcggttgaac
     1561 tggctgtcgg cgtaggaggc gccgccgatc atggcgcgga cccggccgtc gccgtccagc
     1621 gccaccagcg ccgcctgctg cacgcccttg gacttgtcgc gcacgaggat gcggtgaacc
     1681 gctcgttcgg cctgggtctg gagggtgagg tcgagcgtcg tctcgacgat catgtcctcg
     1741 gtcggttcgc cgaccagttc gcggatctgc ttgtcgagcc agtcgacgaa atactgggcg
     1801 tgctggctgg acagggtgcg ggagacgcgg accggcgagg ccgcggcctc ggcgcgttgc
     1861 tcgggcgtga tgaccccggc gtcgaccatt tcgttcagca ccacggtggc gcggaccccg
     1921 gcccgttcgc tctcggatac cggtgaatag cgtgacgggg ccttgagcag gccggccagc
     1981 agtgcggcct cgcccacggt cagctgtttg gcgctcttgt cgaaataacg ctgggacgcc
     2041 gcctcgatgc cataggcgcc ggcgccgaaa tagacgcggt tcagatagag ggcgaggatc
     2101 tccttcttgg agaatttcat ttccagccag accgccagca tcagctcctg aaccttgcgc
     2161 ttcaggt
//
LOCUS       DASVVN010000130         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_373078,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000130 DASVVN010000000
VERSION     DASVVN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373078"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VF686_01960"
     CDS             <1..1075
                     /locus_tag="VF686_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX8660602.1"
                     /translation="LIQPDDVIWIHDYHMMPLARDLRRRGIKNRIGFFLHVPWPVRQL
                     LVTLPHHRRLVETMFDYDLIGFQTREWLDLFRDYVASEPSVHGRLLPNDGLEAFGKTV
                     QTGVFPIGIDVDGFIAARNSVQGNRTYDRMAASAAFRSLIVGVDRLDYSKGLEQRLLG
                     YEQFLETHPDMRSQVLLLQVTPISRDEVDAYQDIRGRLDALAGRINGEYAEMDWQPIR
                     YLNRTYRRDQLAGIYRAARVCLVTPLRDGMNLVAKEYVAAQNPDDPGVLVLSRFAGAA
                     EQMGEALLVNPFSREEMSDAIQRALTMPLAERKRKWECLMQVVRDTDVGVWRDDFVST
                     LRAIERPANGGDRPAAAASDQAA"
     gene            complement(1063..1668)
                     /locus_tag="VF686_01965"
     CDS             complement(1063..1668)
                     /locus_tag="VF686_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8660603.1"
                     /translation="MLTLFHAPQSRSGRIVWLLEEIGADYDIRYVDIFRAMTLTGARD
                     PANLHPDGKVPALAHDGALVTESAAIALYLTDLHPDANLGAPVGSPDRAAYVTWLAWT
                     AGEMEPAFWGKISGAAENDPNAKARYDAAVARLMTALATGPYLMGDRFTAVDVMIASA
                     LAWGREYVPESDLIDAYLQRALNRPANARAMAKDGALSQAA"
     gene            1812..>2357
                     /locus_tag="VF686_01970"
     CDS             1812..>2357
                     /locus_tag="VF686_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX8660604.1"
                     /translation="MRRADRLFQIIQVLRRSSKPVTADAIATELETSKRSIYRDIATL
                     MGQRVPIRGEAGIGYVLDGGFDMPPLMLTSDEVEAAVLGAQWVAGRGDPALARAARDL
                     IAKIAATVPEKLRPVLLEPAVASAPVWKHAVEVETLDMSRVRAWIHAGRKLRLHYADE
                     QGNETLRVIWPCLVGYRETKRL"
BASE COUNT          364 a          795 c          816 g          382 t
ORIGIN      
        1 gctgatccag ccggacgacg tgatctggat tcacgactac cacatgatgc cgctggcccg
       61 ggatctgcgc cgccggggga tcaagaaccg gatcggcttc ttcctgcatg tgccctggcc
      121 ggtgcgtcag ctgctggtga cgctgccgca ccaccggcgg ctggtcgaga ccatgttcga
      181 ctacgacctg atcggcttcc agacccgcga atggctggac ctgttccgcg actatgtggc
      241 gtccgagccg agcgtgcacg gcaggctgct gcccaacgac ggactggagg ccttcggcaa
      301 gacggtccag accggcgtct tcccgatcgg catcgacgtg gacggcttca tcgcggcgcg
      361 gaactcggtt cagggaaacc gaacctatga ccggatggcg gcgtccgcgg ccttccgttc
      421 actgatcgtc ggcgtggacc ggctggacta ttccaagggg ctggaacagc ggctgctggg
      481 ctatgagcaa tttctggaga cccatcccga catgcggtcc caggtcctgc tgctgcaggt
      541 cacgccgatc tctcgcgacg aggtggatgc atatcaggac atccgggggc ggctggacgc
      601 cctcgcgggc cgtatcaacg gcgaatacgc cgagatggac tggcagccga tccgctacct
      661 gaaccggacc taccgccggg accaactcgc cggcatctac cgcgccgccc gggtctgtct
      721 ggtgacgccg ttgcgcgacg ggatgaacct ggtggccaag gaatatgtcg cggcccagaa
      781 tccggacgat ccgggcgtcc tcgtcctgtc ccgcttcgcc ggcgcggccg aacagatggg
      841 cgaggccctg ttggtcaatc cgttcagccg cgaggagatg tcggacgcca tccagcgcgc
      901 cctgaccatg ccgctggccg agcgcaagcg gaagtgggag tgcctgatgc aggtggtgcg
      961 cgacaccgat gtgggcgtgt ggcgcgacga cttcgtttcg accctgcggg cgatcgaacg
     1021 ccccgcgaac ggcggcgacc gtccggccgc cgccgcctcc gatcaggcgg cctgactgag
     1081 cgcgccgtcc ttcgccatcg cccgcgcatt ggccggccgg ttcagcgccc gctgcagata
     1141 ggcgtcgatg aggtcgctct cgggaacgta ctcgcggccc caggcgagcg ccgaggcgat
     1201 catgacgtcc accgccgtga accggtcgcc catcagatac ggccccgtcg ccagggccgt
     1261 catcagacgc gcgacggcgg cgtcatagcg ggccttggcg ttggggtcgt tctcggccgc
     1321 gccgctgatc ttgccccaga aggccggctc catctcgccc gccgtccagg ccagccaggt
     1381 cacataggcc gcgcggtccg gcgagccgac cggcgccccg aggttcgcgt ccgggtgcag
     1441 gtcggtgaga tacagggcga tcgcggccga ttcggtcacc agcgccccgt cgtgcgccaa
     1501 cgccgggacc ttgccgtccg ggtgcagatt ggcgggatcg cgggcgcccg tcagggtcat
     1561 ggcgcggaag atgtcgacat agcggatgtc gtagtcggcc ccgatctcct ccagcagcca
     1621 gacgatgcgg cccgaccgcg actggggagc gtggaacagg gtgagcatgg ttatctcctt
     1681 tgtggcgcta tcgcttggcg cgcggcgcct cgctgcttga gcgcttcggc ggcccctcgc
     1741 cgtcgatgga gattgactac tgccaccctg ctgacagcat gctgtcagca gtcgtcatcc
     1801 atggtggacg aatgagacgc gccgaccgac tgttccagat catccaggtg ctgcgccggt
     1861 cgtcgaagcc ggtcacggcc gacgccatcg cgaccgagct ggaaacctcg aaacgctcca
     1921 tctatcgcga catcgccacc ctgatgggtc agcgggtgcc gatcaggggc gaggcgggca
     1981 tcggctatgt gctggacggc ggcttcgaca tgccgccgct gatgctgacg tccgacgagg
     2041 tcgaggcggc cgtgctgggg gcgcaatggg tggccggccg gggcgacccg gccttggccc
     2101 gcgccgcccg cgacctgatc gccaagatcg cggcgacggt gccggaaaag ctgcggcctg
     2161 tcctgctgga gcccgccgtc gccagcgccc cggtctggaa gcacgcggtc gaggtcgaga
     2221 cgctggatat gtcccgggtc cgcgcctgga tccatgccgg ccgcaagctg cggctccact
     2281 acgcggacga gcaggggaat gagaccctgc gcgtcatctg gccgtgtctg gtcggttatc
     2341 gcgaaaccaa acgcctg
//
LOCUS       DASVVN010000131         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_57191, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000131 DASVVN010000000
VERSION     DASVVN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57191"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..626
                     /locus_tag="VF686_01975"
     CDS             114..626
                     /locus_tag="VF686_01975"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX8660605.1"
                     /translation="MRFDLSSAGIMAAAVASALTTVWWMDQAGLRGQAQAATRPVHAS
                     APASGTSAQVLKDLDGHYWADALIEGRAVRVMVDTGASVVALTRDDAARLGLKLTSED
                     FSGSIVTASGPVRAAPVELRAVAVAGARVERVQAMVVEHGLPHSLLGMSYLGRLSAFT
                     ATPASLTLRP"
     gene            complement(623..1447)
                     /locus_tag="VF686_01980"
     CDS             complement(623..1447)
                     /locus_tag="VF686_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8660606.1"
                     /translation="MTALPLPDLLLLLAALIAAGLAGGIVAGLFGVGGGTVIVPAVFY
                     AFEVLGVGGEGNLHTAIGTSLLTIVATSWRSLRAHRSHGVVDEGVLKTWTPWVGLGAL
                     LGAAVAGVTSMEGLAIVYGVCLALIAAQLGLMPERFTLAKELPTGWARRGIGSLIGGL
                     SAMMGIGGGSFGGMMMTLCGRPIHQAVATASGFGVAIGAAATLGFVAFGWDAAGRPPL
                     SLGYVNAPGALVMAVLTTAVAPWGAKLAHALDRRVLRKAFAVYLLATALSVVVKAL"
     gene            1562..2440
                     /locus_tag="VF686_01985"
     CDS             1562..2440
                     /locus_tag="VF686_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660607.1"
                     /translation="MTSIKTLTALAAGAFVLSAGSAAFAQTPYSSTPYGQSQTQTDPI
                     GAILGALFGDRFGVSTTLDSEWSRGRRPLNTNRAQFESRLDADVRSGALSSSAARSLT
                     AEYDALVTLEARYTADGRVTTTERSDLAERYRAFSTRYQAGGQGGGYDDGYDDWRPLA
                     ESRTEFNSRLDAGVRNRTLTRNQANRLATDFNALVQLETTYERNGIDSRERQDLEARW
                     ADLNRRVGDNYDGGYTPDDNYGSDPRGAQIEARIAAGERNGSISRTEASSLRLELRDL
                     TRRWTELEARVEAARR"
BASE COUNT          416 a         1034 c          769 g          323 t
ORIGIN      
        1 tagccaccag ccactccctt aaccccgcgc aaaccatccc ccttcaccgc atccaaaccc
       61 gcccatgcga taccggagcc gtcgcagaag cggcgcttaa ggacgagtag ggcatgcgtt
      121 tcgacctttc ctctgcgggc atcatggccg ccgccgtcgc ctcggccctg accaccgtct
      181 ggtggatgga tcaggccggc ttgcggggtc aggcccaggc cgcgacccgg cccgttcacg
      241 cgtccgcgcc tgccagcggc acatccgccc aggtgctgaa agacctcgac ggccactact
      301 gggccgacgc cctgatcgag ggccgcgccg tgcgcgtcat ggtcgacacc ggcgccagcg
      361 tggtcgccct gacccgcgac gacgccgccc gccttggcct caagctcacg tccgaggact
      421 tctcgggctc gatcgtcacc gcctccgggc cggtccgcgc cgctccggtc gaactccgcg
      481 ccgtcgccgt cgccggcgcc cgcgtcgagc gcgttcaggc catggtcgtc gaacacggac
      541 tgccgcactc cctcctgggc atgagctatc tgggtcgcct gtcggccttt accgccacgc
      601 ccgccagcct gacgctgcgg ccctagagcg ccttcaccac gaccgacagc gccgtggcca
      661 gcagatagac cgcgaacgcc ttgcgcagca cccgcctgtc cagcgcatgg gccagcttcg
      721 cgccccacgg cgccaccgcc gtggtcagca ccgccatcac cagcgcgccc ggcgcattga
      781 catagcccag cgacagcggc ggccggcccg ccgcgtccca gccaaaggcc acgaacccca
      841 gcgtcgccgc ggccccgatc gccacgccga acccggacgc cgtcgccacc gcctgatgga
      901 tcggccgtcc gcacagcgtc atcatcatgc cgccgaaact gccgccgccg atccccatca
      961 tcgccgacag cccgccgatc aggcttccga tgccgcgccg ggcccagccg gttggcagct
     1021 ctttcgcgag ggtgaaccgc tccggcatca gccccagctg cgccgcgatc agcgccaggc
     1081 agacgccata gacgatggcc agtccctcca tcgaggtcac gcccgccacc gccgccccca
     1141 acagggcccc aagaccgacc cacggcgtcc aggtcttcag caccccctcg tccaccaccc
     1201 cgtgcgaccg gtgcgcccgc agcgaccgcc agctggtcgc cacgatggtc agcagcgagg
     1261 tgccgatggc cgtgtgcaga ttgccctcgc ccccgacccc cagcacctcg aaggcgtaga
     1321 agacggccgg cacgatcacc gtgcccccgc ccacgccgaa caggcccgcc acgatcccgc
     1381 cggccagccc cgctgcgatc agggcggcga ggagaagcag cagatcggga aggggcaggg
     1441 cggtcatccg tcctgtctag tctgactcgc gaccgagggg accgggctac agtcacggca
     1501 acctttcctc gccgactccc gttctctccg ccgacaggca accgccggaa agacaagtcc
     1561 catgacctcg atcaagaccc tcacggccct cgcggccggc gccttcgtgc tttcggcggg
     1621 ctcggccgcc ttcgcccaga cgccttacag ctcgaccccc tatggacaga gccagaccca
     1681 gaccgatccc atcggcgcca tcctcggcgc cctgttcggc gaccgcttcg gcgtgtcgac
     1741 cacgctggac agcgaatggt cgcgcggacg tcgcccgctc aacaccaacc gcgcccagtt
     1801 cgaaagccgg ctggacgccg acgtgcgttc gggcgccctc tcctcctcgg ccgcccgcag
     1861 cctgaccgcc gaatacgacg cgctggtcac gctggaggcg cgctacaccg cggatggacg
     1921 tgtcacgacc accgagcgct cggatctcgc tgaacgctac cgcgcgttct cgacccgcta
     1981 tcaggccggc gggcagggcg gcggctatga cgacggctac gatgactggc gtccgctggc
     2041 ggagagccgc acggagttca acagccgcct tgacgccggc gtgcgcaacc ggacccttac
     2101 ccgcaatcag gcgaaccgcc tcgcgaccga cttcaacgcc cttgtgcagc tggaaaccac
     2161 ctacgagcgc aacggcatcg acagccgcga gcgtcaggat ctggaggccc gctgggccga
     2221 tctgaaccgc cgcgtcggcg acaactacga cggcggctac acgccggacg acaactacgg
     2281 ctccgacccg cgcggcgccc agatcgaggc ccgcatcgcc gccggcgagc gcaacggctc
     2341 gatcagccgg accgaagcct ccagcctgcg tctcgagctg cgggacctga cccgccgctg
     2401 gaccgaactg gaagcccgcg tcgaggctgc gcgccgctag gccgcagagg ggcgctgttc
     2461 agatgagcag cgccccctca tgccgcagca accagccctt gcgctcgaga ccgccgccga
     2521 agccggtcaa cgagccgtcg gc
//
LOCUS       DASVVN010000132         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_477865,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000132 DASVVN010000000
VERSION     DASVVN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477865"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VF686_01990"
     CDS             complement(<1..634)
                     /locus_tag="VF686_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8660608.1"
                     /translation="MNPPSVAVLDALKSAAGAGGWTDDPAEIAPWLTEWRNRWQGHTP
                     LMLTPRSTEETARVVAICAEHRVAITPQGGDTGLVGGQIPFGEVLLSTRKLRAIRDVT
                     PLDDAMTVEAGITLLEAQQAAAAADRLFPLSLAAEGTATIGGVVSTNAGGTAVIRYGV
                     MRDLVLGLEAVLPDGRIYRGLKRLRKDNTGYDLKQLLIGAEGTLGIVTAAT"
     gene            691..1131
                     /locus_tag="VF686_01995"
     CDS             691..1131
                     /locus_tag="VF686_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8660609.1"
                     /translation="MTTALPSPVPAVGVVCLRDDQVLLIRRGTPPRQGEWSLPGGRIE
                     PGERAVDAALRELREETGVAAEITGLIDVVDGVFPEAGRHYVLIDYAARWISGEPVAG
                     DDAVEARFVPLDQVETLVSWAETHRIIALAAASRDLGRDRRSPT"
     gene            1176..1520
                     /locus_tag="VF686_02000"
     CDS             1176..1520
                     /locus_tag="VF686_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660610.1"
                     /translation="MHIRSLVAALAASAALLAPAATAQTESIDFTLTNNTEHVLTHLY
                     ISLPSTNEWEEDIFGADVLGSGESMDISIDDGLPECVYDVRADFSDGDSVQVAEVNFC
                     ELEGEELVINEE"
BASE COUNT          234 a          521 c          533 g          251 t
ORIGIN      
        1 gggtcgcggc ggtgacgatc cccagcgtgc cctcggcgcc gatcagcagc tgtttcaggt
       61 cgtagccggt attgtccttc cgcagccgtt tcaggccgcg gtagatccgg ccgtccggca
      121 gcaccgcctc cagacccagc accagatcgc gcatcacgcc gtagcggatg acggcggtgc
      181 ccccggcatt ggtcgagacc acgccgccga tggtcgccgt gccctcggcc gcgaggctga
      241 gcgggaacag gcggtcggcg gcggcggcgg cctgctgggc ctccagaagg gtgatcccgg
      301 cctcgacggt catggcgtcg tccagcgggg tcacatcgcg gatcgcgcgc agcttgcggg
      361 tcgagagcag gacctcgcca aagggtatct gaccgccgac caagcccgtg tcgccgcctt
      421 ggggcgtgat ggcgacgcgg tgttcggcgc agatggcgac cacccgcgcc gtctcctccg
      481 tcgagcgcgg ggtcagcatc agcggcgtat gtccctgcca gcggttgcgc cattcggtca
      541 gccatggcgc gatctcggcg ggatcgtcgg tccagccgcc ggcgccggcc gcggatttca
      601 gggcgtctag tacggcgacc gagggtgggt tcatgaatcc cttgtctcaa agtcgtggac
      661 gcggcgaaag ggccaagcca cgctggcgcc atgacgaccg cccttccctc gcccgtcccc
      721 gccgtcggcg tcgtctgcct gagggacgat caggtgctgc tgatccggcg tggaacgccg
      781 ccccggcagg gcgaatggag cctgcccggc ggccggatcg agccgggcga acgcgccgtc
      841 gacgccgcgt tgcgtgaact gcgcgaggaa accggggtcg cggccgagat caccggcctg
      901 atcgacgtgg tcgacggagt ctttcccgag gcgggccgcc actacgtatt gatcgactac
      961 gccgcccgct ggatatccgg cgagccggtc gccggcgatg acgcggtcga ggcgcggttc
     1021 gtgccgctgg atcaggtcga gacgctggtc agctgggcgg agacccaccg cattatcgcc
     1081 ctggctgcag catcccgcga tttgggacgc gaccggcgtt ctccgacgtg acaagctgtc
     1141 gcctttccgg cgatcggccg gtggggaatt tgaccatgca tatccgtagt ctcgtggcgg
     1201 cgctcgccgc ctcggccgcc ctgctcgcgc cggccgccac ggcccagacc gagtccatcg
     1261 acttcaccct gaccaacaac accgaacatg tgctgaccca cctgtacatc tccctgcctt
     1321 cgaccaacga atgggaagag gacatcttcg gcgccgacgt tctgggctcg ggcgagagca
     1381 tggatatctc gatcgacgat ggcctgccgg aatgcgtcta cgacgtccgc gccgacttct
     1441 cggacggcga ttcggtacag gttgcggagg tcaatttctg cgaactcgaa ggcgaggaac
     1501 tggtcatcaa cgaggaatag gccgggcgtc gatcgccgg
//
LOCUS       DASVVN010000133         5646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_215184,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000133 DASVVN010000000
VERSION     DASVVN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215184"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2306)
                     /locus_tag="VF686_02005"
     CDS             complement(<1..2306)
                     /locus_tag="VF686_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8660611.1"
                     /translation="MKSRLILTAAASALLLGLPVAAPALAQTAPAATVAVPPLGFVER
                     TLPNGLKVYTARDTSTANVTVQVWYKVGSKDDPAGRSGFAHLFEHLMFKATKNFPDET
                     FDRLTEDVGGNNNAFTSDDVTAYHETIPANHLQRLLFAEADRMSSLVVGEDVFRSERA
                     VVQEEYRQGILASPYGRLFGVFEPATVYQESPYRRTTIGSIEDLDAATLEDVRRFHAT
                     YYRPDNAYLIVAGNFDQAQLDAWIEQYFAPIANPDRPLPANNVVEPEPAGPRDLTFHA
                     PNVPLPAVMLAWPTVKYADPDRAALTVLDGILSTGESSRLYRSLVYDKEIAAQIGSSP
                     DMKQQAGHLTAYAIMADGHTADEGVAALNAEIARFRDEPVTDAELAEAKNELVADALR
                     SRETIDDRANVLGSALIGTNGDASAADREIADIQAVTAADVQRVARRYLTPQRAATIR
                     YLASDEANPTTEQNTAVDAPVRVADLAPVGEIFALLPEAERARMPEIAEAVSPSTPAI
                     ADFRLDNGLRVLVAPKSGLPLVSARLSFDAGSADEAAGKAGAASLTANLLTQGTATRS
                     APQIATEIEQLGANVGAGAGPDFTNVFANAPANVFPQAVALMADLVRNPAFAEEELAR
                     QRTQTLDGLKVALSTPGQVLSQAVGRVVYGNAPYGSPGIGTVTSLPTITRDDIVGFHS
                     ARYRPEAATLVFSGDITEAQARALAQSAFGDWTPAGSAATPTASPAGQTVAPRVMVID
                     QPGAGQAAVAVAMRSVPRATADYFPLTLG"
     gene            complement(2404..4002)
                     /locus_tag="VF686_02010"
     CDS             complement(2404..4002)
                     /locus_tag="VF686_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX8660612.1"
                     /translation="MSWTRRNAFSLLGSATAVPLLGAGKAEAQAPASPMDWARGVEGQ
                     RKADLGDGRYLNPIMAGDHPDPSILHDGDDWYMTFSSFESVPGLVIWRSRDLLNWTPV
                     TAALTRNIGSVWAPELCKHRDRFYLYIPARTPERRTIRVSWSDRIEGPWSDPVDLNLP
                     DHIDPGHAVGEDGHRYLFLSGGDLVRLDDDGLATSGAVRHVYDPWRYPDDWDVESFSP
                     EGPKITRRGDWFFMVTAVGGTAGPPTSHMVIVARSRSVFGPWEDHPDNPLVRTRDRSE
                     PWWSKGHATFVEGPAGDWWMVYHAYENGYWTLGRQTLLEPIVWRADGWPAATGADLSR
                     PQAGPRGGQALPHGAALSDDFSGDRFGVQWSFHNPGPDERARFVSGDGVLRLKAAGAS
                     PRDGSPITFVQGEHAYQIEVEIEVDPGVTAGVLLFYDSALYAGLGFDAARFVTHQYGL
                     ERGRPANPHGGRMWLRMTNDRHVVTFHHSADGRVWKKFDRQMEVSGYHHNVRGGFMAL
                     RPGLYAAGGAGEARFRNFRYRALP"
     gene            complement(4050..4937)
                     /locus_tag="VF686_02015"
     CDS             complement(4050..4937)
                     /locus_tag="VF686_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX8660613.1"
                     /translation="MLPTLRQLQYLKLLAEHASFSRAAEAAHVSQPALSAGVQELEKI
                     LGAPVVERTRGNVQLTAVGAEAVRRAEDVLARTEDLVEAAKNAGKPLSGRFRLGVIPT
                     VAPFLLPTKLPGIRLAYPALRLFIREDLTPRLIAGLKAGQLDAAVIALPYSSSGVDHA
                     RIGDDEILAAAPLGHALAGTGPIPAGSLKAEDLILLEDGHCLRDQALAAFDIDPPKGD
                     DVFAATSLHTLVQMVSAGLGVSFLPEMAVRAGLADMPGVVVRPIAAKAPRREIVVAWR
                     TGSSRAAEARLLAEALTLD"
     gene            complement(5002..>5646)
                     /locus_tag="VF686_02020"
     CDS             complement(5002..>5646)
                     /locus_tag="VF686_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX8660614.1"
                     /translation="SIADLIVLGGSVGVEQAARAAGHQVEVPFKPGRTDASQEQTDVE
                     GFAVLEPKADGFRNYLSVRFNVPTEELLVDRAQLLGLTAPEMTVLVGGLRVLGANHGG
                     SKHGVFTDRPGQLTNDFFINLLDMRTGWKEVDDSGDETFVGTDRLTGEQLWTATRTDL
                     VFGSNSQLRALSEVYASADAGQKFVKDFVAAWTKVMNADRYDITLDEEFGVAAV"
BASE COUNT          859 a         2019 c         1941 g          827 t
ORIGIN      
        1 cccagggtca gcgggaaata gtccgccgtc gcccgcggca cgctgcgcat ggccaccgcc
       61 accgccgcct gtccggcgcc cggctggtca atgaccatga cgcgcggcgc cacggtctgt
      121 ccggccgggg aggcggtcgg cgtcgcggcc gaaccggccg gcgtccagtc gccgaacgcc
      181 gactgcgcca gcgcccgggc ctgcgcctcg gtgatgtcgc ccgagaacac caaagtcgcc
      241 gcttcggggc ggtagcgggc cgaatggaag ccgacgatgt cgtcgcgcgt gatcgtcggc
      301 agggaggtca cggtaccgat gcccggcgag ccatagggcg cattgccgta gacgacgcgg
      361 ccgacggcct ggctcagcac ctgtcccggc gtgctcagcg ccaccttcag accgtcgagg
      421 gtctgggtcc gctgacgggc cagctcctcc tcggcgaagg ccggattgcg caccagatcg
      481 gccatcaggg cgacggcctg cgggaagaca ttggccgggg cgttggcgaa gacgttggtg
      541 aagtccggac ccgcgcccgc gccgacgttg gcgcccaact gctcgatctc ggtggcgatc
      601 tgcggggccg agcgcgtggc cgtaccctgc gtgagcagat tggcggtcag gctggccgcg
      661 ccggccttgc ccgccgcctc gtcggccgaa ccggcgtcga agctcagacg cgccgagacc
      721 agcggcaggc cgctcttcgg cgccaccagc acgcgcaggc cgttgtcgag gcggaagtcg
      781 gcgatggccg gggtcgaggg cgacaccgcc tcggcgatct ccggcatccg ggcgcgctcg
      841 gcctcgggca gcagggcgaa gatctcgccc acgggggcca gatcggcgac ccgcaccggc
      901 gcgtcgacgg cggtgttctg ttcggtcgtc ggattggcct cgtctgaagc cagataacgg
      961 atggtcgccg cgcgctgtgg ggtcagatag cgccgcgcca cccgctgcac atcggcggcg
     1021 gtcacggcct ggatgtcggc gatctcgcgg tcggcggccg aggcgtcgcc attggttccg
     1081 atcagggccg agcccagcac attggcgcgg tcgtcgatgg tctcgcggct gcgcagggcg
     1141 tcggcgacca gctcgttctt ggcctcggcc agttcggcgt cggtgaccgg ctcatcgcgg
     1201 aaacgggcga tctcggcgtt cagggcggcg acgccctcgt ccgccgtatg gccgtcggcc
     1261 atgatggcgt aggcggtcag gtgcccggcc tgctgcttca tgtcgggcga ggagccgatc
     1321 tgggccgcga tctccttgtc atagaccagc gagcggtaca ggcgcgagct ctcgccggtc
     1381 gacaggatgc cgtccagcac ggtcagggcg gcgcgatccg ggtccgcgta tttgaccgtc
     1441 ggccaagcca gcatgacggc gggcaggggc acgttcggcg catggaaggt caggtcgcgc
     1501 ggacccgccg gttccggctc gaccacattg ttggccggca gcggacggtc gggattggcg
     1561 atcggcgcga aatactgctc gatccaggcg tccagctggg cctgatcgaa attccccgcc
     1621 acgatcagat aggcgttgtc gggccggtag taggtggcgt ggaaccgccg cacgtcctcc
     1681 agcgtcgccg cgtccagatc ctcgatcgag ccgatggtcg tccggcgata ggggctttcc
     1741 tgatagacgg tggccggttc gaacacgccg aacagccggc cgtaggggct ggccagaatg
     1801 ccctggcggt actcttcctg caccaccgcg cgctcggagc ggaacacgtc ctcgccgacg
     1861 accagcgagc tcatccggtc ggcctcggcg aacagcagcc gctgcaggtg attggccgga
     1921 atggtctcgt gataggccgt cacgtcgtcc gaggtgaagg cgttgttgtt gccgcccaca
     1981 tcctcggtca gacggtcgaa ggtctcgtcg gggaaattct tcgtcgcctt gaacatcagg
     2041 tgttcgaaca ggtgggcgaa acccgaacgc ccggccggat cgtccttgga cccgaccttg
     2101 taccagacct gcaccgtgac attggccgtc gacgtatcgc gcgccgtgta gaccttcagc
     2161 ccgttcggca gggtgcgctc gacaaagccg agcggcggca cggccaccgt ggcggcgggc
     2221 gcggtctgcg ccagggcggg cgcggcgacc ggaaggccga gcagaagggc cgaggcggcg
     2281 gcggtcagga tcaggcggga cttcatggga cgaggggctc cgaaagcccg acgcacgcgg
     2341 gctcgcggac cctatcagcg ggccgtccgc gcgcacctta cggagtcgta atgatcgcca
     2401 gcgctacggc agggcgcggt agcggaagtt ccggaaccgc gcctcgcccg cgccgcccgc
     2461 cgcatacagg ccggggcgca gcgccatgaa gccgccgcgc acattgtggt ggtagccgga
     2521 cacctccatc tgccggtcga acttcttcca cacccgcccg tcggcgctgt ggtggaaggt
     2581 gaccacatgc cggtcgttgg tcatccgcag ccacatccgc ccaccgtgcg gattggccgg
     2641 gcggccgcgc tccagcccgt actgatgcgt cacgaaccgc gccgcgtcga agcccagccc
     2701 ggcgtagagc gcgctgtcgt agaacagcag caccccggcc gtgacgcccg gatcgacctc
     2761 gatctcgacc tcgatctgat acgcgtgctc gccctgcaca aaggtgatcg gcgagccgtc
     2821 gcgcggcgag gcgcccgccg ccttgagccg cagcaccccg tccccgctga cgaaccgcgc
     2881 ccgctcgtcc ggtcccggat tgtggaagct ccactgcacg ccgaagcggt cgccgctgaa
     2941 gtcgtccgac agggccgcgc catgcggcag cgcctgtccc ccgcgcggcc cggcctgcgg
     3001 ccgcgacagg tcggcgccgg tcgccgccgg ccaaccgtcc gcgcgccaga cgatcggctc
     3061 cagcaacgtc tgccggccca gcgtccagta gccgttctca taggcgtgat agaccatcca
     3121 ccagtcaccc gccggcccct cgacgaaggt ggcgtggccc ttggaccacc acggctcgga
     3181 ccggtcgcgg gtgcggacca gcggattgtc ggggtgatct tcccacgggc cgaacaccga
     3241 tcgcgatcgg gccacgatga ccatatggct ggtcggcggc cccgcggttc cgcccaccgc
     3301 cgtgaccatg aagaaccagt cgcctcgccg cgtgatcttg ggcccttccg gcgaaaagct
     3361 ttcgacgtcc cagtcgtccg gatagcgcca cggatcatag acgtgccgca ccgcacccga
     3421 agtggccagg ccgtcgtcgt ccagccgcac cagatcgccg ccgctgagga acagataccg
     3481 gtgaccgtcc tcgcccaccg catggcccgg atcgatatgg tcgggcaggt tcagatcgac
     3541 gggatcgctc cacggccctt cgatccggtc ggaccagctg acccggatgg tccgccgctc
     3601 gggcgtccgc gcggggatgt agaggtagaa ccggtccctg tgcttgcaca gctccggcgc
     3661 ccagaccgag ccgatgttgc gcgtcagggc cgccgtcacc ggcgtccagt tcagcaggtc
     3721 gcgcgagcgc cagatgacca gcccgggcac cgactcgaac gacgagaagg tcatgtacca
     3781 gtcgtcgccg tcgtgcagga tcgacgggtc cggatggtcg cccgccatga tcgggttcag
     3841 gtagcggccg tcgccgaggt cggccttgcg ctgaccctcg acgccgcggg cccaatccat
     3901 gggactggct ggcgcctgag cctcagcctt gccggcgccc agcaacggaa cggccgtcgc
     3961 cgagcccagc aggctgaagg cgtttctgcg cgtccatgac atcggccgtc tcctgtcccg
     4021 ggcacacgat gacaccggtg gacagggcgt caatccagag tcagcgcctc ggccagcagc
     4081 cgggcctcgg cggcgcggct ggaacccgtc cgccatgcga ccacgatctc gcggcgcggg
     4141 gccttggccg cgatcggccg caccaccacg ccgggcatgt cggccagccc cgcccgcacc
     4201 gccatctcgg gcaggaagct gacgcccagc cccgccgaga ccatctgcac cagcgtatgc
     4261 agcgacgtcg cggcgaacac gtcgtcgccc ttgggcggat cgatgtcgaa ggccgccaag
     4321 gcctgatcgc gcaggcagtg gccgtcctcc agcaggatca gatcctcggc cttcagcgag
     4381 ccggcgggga tcgggccggt cccggccagc gcatggccca gaggcgcggc ggcgaggatt
     4441 tcatcgtcgc cgatgcgcgc gtggtccacg ccgcttgagc tgtagggcag ggcgatcacc
     4501 gccgcatcca gctgcccggc cttcagcccg gcgatcagtc gcggcgtcag atcctcgcgg
     4561 atgaacagcc gtaaggccgg ataggccaac cttatcccgg gaagcttggt cggcagcagg
     4621 aagggggcga ccgtcggaat gacgcccaac cggaaccggc ccgacagcgg cttgcctgca
     4681 ttcttcgccg cctcgaccag atcctcggtc cgcgccagca cgtcctcggc ccgccgcacc
     4741 gcctcggccc cgaccgcggt cagctggacg ttgccgcgtg tgcgctcgac aaccggcgcg
     4801 ccgagaatct tctccagctc ctgcaccccg gccgacagcg ccggctggct gacatgcgcc
     4861 gcctccgccg cccggctgaa cgaggcgtgt tcggccagga gcttgagata ctggagctgt
     4921 cgcagggtcg ggagcatgcg cccgacatag gccgtcgcta tggcgaaagc catgccgatc
     4981 cacccgccct atgtggcgac cctacaccgc cgccacgccg aactcctcgt ccagggtgat
     5041 gtcgtagcgg tcggcgttca tcaccttggt ccaggccgcc acgaaatcct tgacgaactt
     5101 ctgccccgca tcggccgagg catagacctc cgacagcgcc cgcaactgcg agttggagcc
     5161 gaacaccaga tcggtgcgcg tcgccgtcca cagctgttcg ccggtgagcc ggtccgtgcc
     5221 gacgaaggtc tcgtcgccgc tgtcgtcgac ctccttccag ccggtccgca tgtccagcag
     5281 attgatgaag aagtcgttgg tcagctggcc cggacggtcg gtgaacacgc cgtgcttcga
     5341 gccgccgtgg ttggcgccca gcacgcgcag cccgccgacc aacaccgtca tctccggcgc
     5401 cgtcagcccc agcagctggg cccggtcgac cagcagctcc tcggtcggca cgttgaaccg
     5461 caccgacaga tagttgcgga agccatcggc cttgggctcc agcaccgcga agccctcgac
     5521 gtcggtctgc tcctgactgg cgtcggtgcg gcccggcttg aacggaacct cgacctggtg
     5581 gccggcagca cgcgcggcct gctcgacgcc gaccgaaccg cccagcacga tcaggtcggc
     5641 gatgga
//
LOCUS       DASVVN010000134         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_399771,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000134 DASVVN010000000
VERSION     DASVVN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399771"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VF686_02025"
     CDS             <1..807
                     /locus_tag="VF686_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8660615.1"
                     /translation="FWRFAFALPLLLMLTMRPKSLMGGGEGIGRPSGWMALAGGMLVF
                     DLAFWHYSIAFTSVANATTLTNLTPVVVTLVGWWVFRDRPRALFLVALAAAIAGAVTM
                     SLGADGGQGSNPPLGDAFAAVTALWYAGYFIAVKAARGRYSAGRVMLWSTAIAAPGLL
                     LAALVLGEQVLPGSSRGWAACAGLGLMHVVGQGGVAWSLGRLPAALTAVTVLIQPVVA
                     ALLGWLIFAETLTPVQALGGAVVLAAIVLAQWSSTRKKTGAEAEAAAPVP"
     gene            complement(824..1027)
                     /locus_tag="VF686_02030"
     CDS             complement(824..1027)
                     /locus_tag="VF686_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8660616.1"
                     /translation="MATGTVKWFNPTKGFGFIQPDGGGQDVFVHITAVQKAGLQGLDE
                     NAKVEYELESQRGKTSAIDLKIL"
     gene            complement(1231..>1564)
                     /locus_tag="VF686_02035"
     CDS             complement(1231..>1564)
                     /locus_tag="VF686_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX8660617.1"
                     /translation="AFGSRVSRINEVGTYSCRRIYGLTTTDARPSEHARANALDIGGM
                     TLADGRRVSVLSDWDGTGPRGAEGQSFLKRIRDGACRAFSTVLTPDYNEAHANHLHLD
                     GAPRSLCA"
BASE COUNT          202 a          489 c          592 g          281 t
ORIGIN      
        1 ttctggcggt tcgcgttcgc cttgccgctg ctgctgatgc tgacgatgcg gcccaaatcc
       61 ctgatggggg gcggagaagg gatcgggcgg ccgtccgggt ggatggcgct ggcgggcggg
      121 atgctcgtgt tcgacctggc cttctggcac tattcgatcg ccttcacctc ggtggccaac
      181 gccaccaccc tgaccaacct gacgccggtg gtggtgaccc tggtcggctg gtgggtgttc
      241 cgcgaccggc cgagggcgct gttcctcgtg gccctggccg cggccatcgc gggggccgtg
      301 acgatgtcgc tgggagccga cggcgggcag ggctcgaatc ctccgctggg cgacgccttc
      361 gccgccgtca ccgccctttg gtacgccggt tatttcatcg ccgtgaaggc ggcgcgcggg
      421 cggtattcgg ccggacgggt gatgctgtgg tcgaccgcca tcgccgcgcc gggcctgctg
      481 ctggccgcgc tggtgctggg cgagcaggtc ctgcccggat cgtcgcgcgg ctgggccgcc
      541 tgcgcgggtc tggggctgat gcatgtggtc gggcagggcg gggtggcgtg gtcgctgggc
      601 cggctgccgg cggcgttgac ggcggtgacg gtgctgatcc agcccgtggt cgcggccctg
      661 ctgggctggc tgatcttcgc cgagaccctg acgccggttc aggcgctggg cggggccgtg
      721 gtgctggcgg cgatcgtgct ggcgcagtgg tcgagcaccc gaaagaaaac gggcgcggaa
      781 gccgaagccg ccgcgcccgt cccgtaatgc tgtcagtcgt ggatcagagg atcttgaggt
      841 cgatcgccga cgtcttgccg cgctgggatt ccagctcgta ttcgaccttg gcgttctcat
      901 ccaggccttg caggcccgcc ttctgaaccg cggtgatgtg gacgaaaacg tcctggccgc
      961 cgccatcagg ctggatgaag ccgaaaccct tggtcgggtt gaaccacttg accgtaccgg
     1021 tcgccataat gcgtctccgt gaacgcgctt gccaggcagg cgtcccgacg ggacacccgt
     1081 cagcccatct ggacgagctc attcagcgaa ggagcgggca cgggtgtgga ggccgcgcga
     1141 aatccggact gcggcgactt tctctcgcgc aatttcaggg cggtcaacct gaaaccgacg
     1201 gccgtgttgg gcgagcatcg ttacgtgaaa tcacgcgcag aggctgcgcg gggcgccgtc
     1261 cagatgcagg tggttggcgt gcgcctcgtt gtagtcgggt gtcagaacgg tcgaaaacgc
     1321 gcggcaggcg ccgtcgcgga tgcgtttgag gaagctctgg ccctcggcgc cgcgggggcc
     1381 ggtcccgtcc cagtcgctca gcaccgagac cctgcggccg tcggccaggg tcatgccgcc
     1441 gatgtcgagc gcattggcgc gtgcgtgctc gctgggccgg gcgtcggttg tggtgaggcc
     1501 gtagatgcgg cggcaggaat aggtgccgac ctcgttgatg cgagagaccc gactgccgaa
     1561 cgcc
//
LOCUS       DASVVN010000135         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_267724,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000135 DASVVN010000000
VERSION     DASVVN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267724"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1486)
                     /locus_tag="VF686_02040"
     CDS             complement(17..1486)
                     /locus_tag="VF686_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8660618.1"
                     /translation="MTDLSVEHSHSLLKPGINCWRVEPAGRFAVLMENAAYFEALRSA
                     LAKARKSVVILGWQFDPRTRLDPESPHNDHQAQIGHQLRMLVKSNPELDVRLLIWKSP
                     LLIAASQGFYPHRAQGWFRKRMVEFRLDQPGPIGACHHQKAVIIDDAVAFCGGGDIST
                     DRWDSEEHLDGDPRRCQPSGEIPSPRHEVMTVMDGPAARALGDLARERWFRSTWERTV
                     PDEVDSDPWPDGVEPDMRDVPVAIARTEPATGGRTEVRENEALHLDAIRGAKKLIYFE
                     NQYFTSPVIAAALAERLAEEDGPEIVLVSTGSSPSWFDRMTMDTARSEILFRLEQADR
                     NNRFFAFTPYTKGGERIIVHAKVSIYDDAVLRVGSTNLNNRSFGFDTECDVAAEPRDQ
                     AGRDAIRAFRHRTIGHWIGIQGAEFAAAEATAGSVGEAIQRFDTGRMHTLGAEPPSRA
                     ERIIAEFQLGDPTSTRDAFRPWKRRTRSHRTLPAIPGPA"
     gene            1675..1863
                     /locus_tag="VF686_02045"
     CDS             1675..1863
                     /locus_tag="VF686_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660619.1"
                     /translation="MQALIEFIAGFVALLAAAALSQFGLDLDPPQKSEREIHRVRDCD
                     NGPSSAVFSASSERKQNC"
     gene            2113..>3691
                     /locus_tag="VF686_02050"
     CDS             2113..>3691
                     /locus_tag="VF686_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tipN"
                     /protein_id="HEX8660620.1"
                     /translation="MSERRRRRLAEDQRRAEERAAAEAVRQDPWPAGPMAELAVSADA
                     PPVAYAPPPMPAAPVGRREHAAPSGRHAYLVAGVASALWVGGVAAWFMYEFGAGAVEL
                     EPLRLAVYALIALAPAGMAIMLAHAVRQGAGLAAETRRARELSEALIAPTALAAQQTG
                     EVLQSLRHDIDQASLAAERARNDMALLREALSQETTRLNEAAETAGRTARRLVEELGG
                     ERTHMAALGTQLDAQASGVLDAVERQSRMVVDASDLAQPQLREAEAALAARAADLAAA
                     AGEAQDAARVAADDLARQTLRLENAGSGVAEQIQSVEEGLGQQRAALVTAAYALRTDQ
                     EDFSAQVESQRAQLTEQLSASRSAAGELGDTSTRTAETIRDLVEAATDQFRALVEMSQ
                     READGFDSATKLALDRFEALAAEARDALVEETRLALSTLQATAEESRAAAADAAEQAR
                     MRADRLGEALFEAAQKADQAADARVDSARKIVTETGGLVDEAGQRALERLESTLERMN
                     AALSQIDETISDLDERAA"
BASE COUNT          573 a         1323 c         1242 g          553 t
ORIGIN      
        1 atcatcgatt tcgagatcag gccggcccgg ggatagccgg cagggtacgg tgcgaccggg
       61 tgcggcgctt ccacggccgg aaggcgtccc gggtcgaggt cggatcaccc agctggaatt
      121 cggcgatgat ccgctcggcg cgcgagggag gctcggcgcc gagcgtgtgc atccggcctg
      181 tgtcgaaccg ctgaatggcc tcaccgaccg atccggccgt cgcctcggcc gccgcgaact
      241 ccgccccctg gatgccgatc cagtgaccga tagtccgatg acggaaggcg cgtatggcgt
      301 cgcgacccgc ctgatcgcgg ggttccgcgg cgacgtcgca ttccgtgtcg aagccaaagg
      361 accgattgtt caggttcgtc gacccgacgc gcaggacggc atcgtcgtag atcgagacct
      421 tggcgtggac gatgatccgt tcgccgccct tggtgtaggg tgtgaaggcg aagaagcgat
      481 tgttccgatc cgcctgctcg agtcggaaga ggatttccga ccgcgccgtg tccatggtca
      541 tgcggtcgaa ccagctgggg ctgctgcccg tcgagaccag gacgatttcc ggcccgtcct
      601 cctccgccag ccgctcggcg agcgccgccg cgatgacagg cgaggtgaaa tactggttct
      661 cgaaatagat cagcttcttc gcgccgcgga tggcgtcgag atgaagcgcc tcgttctcgc
      721 gcacctcggt tcgcccgccc gtcgccggct cggtgcgcgc gatcgcgacc ggcacatcac
      781 gcatgtcggg ctcgacgccg tcgggccagg gatcgctgtc cacctcgtcc ggcaccgtcc
      841 gttcccaggt cgagcggaac cagcgctccc gcgccaaatc cccgagcgcg cgcgcggcgg
      901 ggccgtccat gacggtcatg acctcatgcc ggggcgacgg gatttcgccg gacggctggc
      961 accgccgcgg gtcgccgtcc agatgttctt ccgaatccca gcgatcggtc gagatgtccc
     1021 cgccgccgca gaacgccacg gcgtcgtcga tgatgaccgc cttctggtgg tgacaggccc
     1081 cgatagggcc cggctgatcc aggcggaact cgaccatccg cttgcggaac cagccctgcg
     1141 ccctgtgagg gtagaaccct tgcgacgccg cgatcagcag gggcgatttc cagatcagca
     1201 accggacatc cagctccggg ttggacttga ccagcattct gagctgatgc ccgatctggg
     1261 cctgatgatc gttgtgcggg ctttcgggat ccagccgcgt ccgggggtcg aactgccagc
     1321 ccagaatcac caccgacttc cgggccttgg ccaaggccga gcgcagagcc tcgaaatagg
     1381 cggcgttttc catcagcacc gcgaaacggc cggcgggctc gacccgccag cagttgatgc
     1441 ccggtttcag caggctgtgg ctgtgttcga cactcaggtc agtcatggcg atggtttggc
     1501 ccaatcccgc ggcgggcgcc gtacgcctca agctctacag gacgcgccag ccacttccgt
     1561 tccaccccgc aacgcagatc gtcaatggtg acgctttgtt cacgcgctga cggattgtcc
     1621 gaacggcgtt ttaagcgcat aagtttgctc gcagtcggga cagggagatc ggcgatgcag
     1681 gcgctgatcg aatttatcgc gggtttcgtg gcccttcttg ccgctgcggc cttgtcgcag
     1741 ttcggactcg acctcgaccc gccgcagaaa tccgagcgcg agatccaccg ggtgcgcgat
     1801 tgcgataacg gcccgtcgtc ggcggtgttc tccgcctcgt ccgagcgcaa gcagaactgc
     1861 tgatccgccg ggcacagact gtcgccggaa tcctttgtcc ccgcctttgc gcgccgcgat
     1921 tgactcgtta gggtcacagc gtcctcccgc gcgtcggtcg ccgacgccgc tccgtgacga
     1981 gatcgccgca ccatgaaacc accccgccaa cggccaccgc tgaacctgac cgaaccggat
     2041 ccggaggtcg gcgccgccga ctccgcgccg gaggccgttc agccaacggc cgagcccgtg
     2101 gcggagcgtc ccatgtccga acgccgcaga cgccggctgg ccgaggacca gcgccgcgcc
     2161 gaggaacgcg cggctgccga agccgtccgc caggatccct ggcctgccgg tccgatggcg
     2221 gaactggcgg tgtccgccga cgcgccgccg gtcgcctacg cgccgccgcc catgcctgcc
     2281 gcgccagtcg gccgtcgcga gcatgccgca ccctccggcc gccacgccta tctggtcgcc
     2341 ggcgtcgcct cggccctgtg ggtcggcggc gtcgcggcat ggttcatgta cgaattcggc
     2401 gcgggcgccg ttgaactgga gcccctgcgt cttgcggttt acgccctgat tgcgctggcc
     2461 ccggcgggca tggcgatcat gctggcccac gccgtccgac agggcgccgg tctggccgcc
     2521 gagacccgcc gcgcccgtga actgtccgag gcgctgatcg cccccaccgc cctggcggcc
     2581 caacagaccg gagaggtcct tcagtccctg cgtcacgaca ttgatcaggc ctcgctggcg
     2641 gcggaacggg cccgcaacga catggccctg ctgcgcgaag ccctgtcgca ggaaacgacc
     2701 cgtctgaacg aggccgcgga aaccgctggc cgcaccgcgc gacgcctcgt cgaggaactg
     2761 ggcggcgagc gcacccatat ggcggcgttg ggcacgcaac tggatgcgca ggcgtccggc
     2821 gtcctcgacg ccgtcgagcg ccagtcgcgg atggtggtcg acgcgtccga tctggcccag
     2881 ccccagcttc gcgaggccga ggccgccctg gccgcccgcg ccgccgacct ggccgccgcc
     2941 gccggcgagg cccaggacgc cgcccgcgtc gccgccgacg atctggcccg ccagaccctg
     3001 cggctggaaa acgccggctc gggcgtcgcc gaacagatcc agtcggtcga ggaaggtctg
     3061 ggccagcagc gcgccgccct ggtcacggcc gcctacgccc tgcgcaccga tcaggaagac
     3121 ttctccgccc aggtggaaag ccagcgggcg cagctgacgg aacagttgtc ggcaagccgc
     3181 tcggccgctg gcgaactggg cgacacctcg acccgcacgg cggagacgat ccgcgacctc
     3241 gtcgaggccg cgaccgatca gttccgcgcc ctggtcgaga tgtcccagcg cgaggcggac
     3301 gggttcgact cggccaccaa gctggcgctc gaccggttcg aggccctcgc ggccgaagcc
     3361 cgtgacgccc tggtcgagga gacccgactg gcgctgtcga cgttgcaggc caccgccgag
     3421 gaatcacgcg ccgccgccgc cgatgcagcg gaacaggccc ggatgcgggc ggaccgtctg
     3481 ggcgaagccc tgttcgaagc agcccagaag gccgatcagg ccgccgacgc ccgcgtcgac
     3541 agcgcccgca agatcgtgac cgagaccggc ggactggtcg acgaggccgg ccaacgcgcc
     3601 ctcgagcgtc tggagtccac gctggagcgc atgaacgccg ccctgagtca gatcgacgag
     3661 acgatcagcg atctggacga acgcgccgcc c
//
LOCUS       DASVVN010000136         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_241739,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000136 DASVVN010000000
VERSION     DASVVN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241739"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VF686_02055"
     CDS             <1..607
                     /locus_tag="VF686_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HEX8660621.1"
                     /translation="LWPARADEAGVEVHLLDNGFHTDLAVPRVALEARAGPLADAVRG
                     LAPGDWILIGWGDAKFYVDQSPMESRLPDGLRAFFRPGNASVIMLDPAQRDPRAAFAP
                     ESRRAFRLSAAGFDAMADHIQNSMALTGGRARIAAARAGDDARFFASREHFSIGHLCN
                     HWSAGVLNAAGLPVRPIRSVTSAEVMATIDRAELDTSAARD"
     gene            719..1237
                     /gene="rbfA"
                     /locus_tag="VF686_02060"
     CDS             719..1237
                     /gene="rbfA"
                     /locus_tag="VF686_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX8660622.1"
                     /translation="MSNRKQKKPAAKAGTVSQRQLRASEMIRHALVEVMRENEIRDPA
                     LVGVSVTVTEVRLSPDLKHATCFVEPLGAGVETAPVAGQIDEIVKALNVHAKFLRGAL
                     GRHIDMRFTPDLRFRHDESFAAAERLNRLLDDPRVRADVYVSPFEDDDAEEDGQDGPQ
                     VAQAATGPKEKP"
     gene            1237..>1558
                     /locus_tag="VF686_02065"
     CDS             1237..>1558
                     /locus_tag="VF686_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX8660623.1"
                     /translation="MGRRKRGAIVNGWVCLDKPYGMGSTDAVSKVRRLFDAQKAGHAG
                     TLDPLASGILPIALGEATKTVPFMMEAQKVYRFTVNWGVTTDSLDREGEIIARSDVRP
                     GVEAV"
BASE COUNT          247 a          547 c          534 g          230 t
ORIGIN      
        1 gctgtggccc gcccgcgccg acgaggcggg ggtcgaggtt catctgctgg acaacggctt
       61 ccacacggat ctggccgttc cccgcgtcgc tctggaagcg cgggccgggc cgctggccga
      121 cgccgtgcgc ggcctggcgc cgggagactg gatcctgatc ggctggggcg acgccaaatt
      181 ctacgtcgac cagagcccga tggagagccg tctgccggac ggcctgcgcg ccttcttccg
      241 gcccggcaac gcatcggtga tcatgctgga cccggcccag cgggacccga gggcggcttt
      301 cgcgccggaa agccgtcgcg ccttccggct ttcggctgcc ggcttcgacg ccatggccga
      361 ccatatccag aattcgatgg ccctgaccgg cggccgagcc cgcatcgccg cggcgcgggc
      421 aggcgacgac gcgcgcttct tcgcgagccg cgagcatttc tcgatcgggc acctgtgcaa
      481 tcactggagc gcgggtgttc tcaatgccgc cggcctgccc gtgcgcccca tccggtccgt
      541 gacgtccgcg gaggtcatgg ccaccatcga ccgcgccgaa ttggacacca gcgccgcacg
      601 ggactagacc ccgcgctctt ttatttttgc cgcgttcgaa aggctgcgcc tttccgcccg
      661 acgcggcttc gctttctcaa gccgggtccg atccccggca ggcgacgagg acagagccat
      721 gagcaaccgc aaacagaaga agcccgccgc caaggccggc acggtcagcc agcgtcagct
      781 gcgcgcctcc gaaatgatcc gtcacgccct ggtcgaggtc atgcgcgaga acgagatccg
      841 cgacccggcc ctggtcggcg tgtccgtgac cgtcaccgag gtgcggctgt cgcccgacct
      901 gaaacacgcc acctgtttcg tcgaaccgct gggcgcgggc gtcgagacgg cgccggtcgc
      961 cggccagatc gacgagatcg tcaaggcgct gaacgtccac gccaaattcc tgcgcggtgc
     1021 gctgggacgg catatcgaca tgcgcttcac ccccgacctg cgcttccgcc acgacgagag
     1081 cttcgccgcg gccgagcgtc tgaaccggct gctggacgat ccccgggtgc gcgccgacgt
     1141 ctatgtgtcg ccgttcgagg atgatgacgc cgaagaggac ggccaggacg gccctcaagt
     1201 agcgcaagcc gcgacagggc cgaaggaaaa gccctgatgg gccgccggaa acggggcgcg
     1261 atcgtcaacg gctgggtctg tctggacaag ccgtacggca tggggtcgac cgacgccgtg
     1321 tcgaaggtcc gccggctgtt cgacgcgcag aaggccgggc atgccgggac gctggacccg
     1381 ctggccagcg gcatcctgcc catcgcgctg ggcgaggcga ccaagaccgt gcccttcatg
     1441 atggaggcgc agaaggtcta tcgcttcacc gtcaactggg gcgtcacgac cgacagcctg
     1501 gaccgcgagg gcgagatcat cgcccggtcg gatgtccggc ccggcgtcga ggcggtgc
//
LOCUS       DASVVN010000137         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_136459,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000137 DASVVN010000000
VERSION     DASVVN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136459"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..580)
                     /locus_tag="VF686_02070"
     CDS             complement(65..580)
                     /locus_tag="VF686_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660624.1"
                     /translation="MLAIVFAVMLQQAAPLPAAIGQVVWEEPAPPAVAAAPVEIPAIP
                     DSARADPYGYERAECSPLIRKASETMEACQARVRIALAANLGAALPPGLAPAGAPDEC
                     RLAAAGDRYALTCGAPDRPARADVRPQEQTCESRPRARPEGGVAWTEECRPADGRAPA
                     EDGLKIKLFGD"
     gene            complement(615..1793)
                     /locus_tag="VF686_02075"
     CDS             complement(615..1793)
                     /locus_tag="VF686_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8660625.1"
                     /translation="MLLPFFTALRDARVPVSMKEWLHLMEAMDKDVAGRKVDDFYHLS
                     RAVLVKDEKHYDRFDQVFGKVFAGIETVGAGDEPSLDVPEDWLRLLNEKYLTDEEKAQ
                     IEALGGFDKLMETLKQRLEEQQGRHEGGNKWIGTGGTSPFGHGGYNPEGVRVGGPGRQ
                     GRAVKVWEKREYRNLDDQVELGTRNIKVALRRLRRFARQGAPDELDMDATIDGTARQG
                     WLDIHMRAERRNTIKVLLFLDIGGSMDAHVKMCEELFSAAKTEFKHLEFFYFHNCLYE
                     GVWKDNRRRHEQKIPTWEVLNKYPADWRAIFVGDATMSPYEVTMPGGSVEHWNEEAGA
                     VWMKRATQQWDKVAWLNPSPERYWSYSASVGMVRELVDERMYPLTLEGLEKAMRALAK
                     "
     gene            complement(1806..2531)
                     /locus_tag="VF686_02080"
     CDS             complement(1806..2531)
                     /locus_tag="VF686_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8660626.1"
                     /translation="MKDVRIGTSGWAYKDWNGPFYPEEVKAAGRLEYISRRFRTLEIN
                     ASFYRMPTDKAVADWRAQTPDDFLFAWKASRFITHNKKLKDPVEPLAYMFERIAGLGD
                     KIGPILFQLPPNLHRNDERLQTFLDALPEGRRYSVEFRHPGWYADEVLEMLHKRNVAL
                     CISDHHHAPAPWEATADFIYLRGHGPGGHYHGRYGEAALKEWARAIRDWRVKREVFVY
                     FDNDIKSAAPADADQLIGLVDKA"
     gene            2700..>3713
                     /locus_tag="VF686_02085"
     CDS             2700..>3713
                     /locus_tag="VF686_02085"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8660627.1"
                     /translation="MTQPDATLLAAVQRRDWAAARPGLEDLAAAGQADARSLAWLATA
                     RARAGDAALAHEAADRALQLEARNLRAALVKADLLWAEGAKREANYFYGAVVEIGGSA
                     SGLEPDMVAGVRRARTIKDSLARDIFAHIRDELGQAGFSESRSSPRFVQAFDLATGRK
                     QPYFQQPKAFYFPELPNTQFYPRDQFPWLDKVEAATDAITAELMAVLEEDAAFAPYIQ
                     TVPNMPTRTDYPLVDSMDWSTCFLWKDGAETPNAARCPKTMAALEDVPLCRISGRSPQ
                     IMFSQLKAGAHIQPHHGYVNTRLVCHLPLIVPPGCRFRVGNDVRSWEKGKAWVFDDTI
                     EHEA"
BASE COUNT          570 a         1325 c         1165 g          653 t
ORIGIN      
        1 ggagctttca attgatggaa gcgttccgtc ccggatcgcc gcttcgcggc gtccgggatg
       61 acaatcagtc cccgaacagc ttgatcttca gaccatcctc ggccggcgcc cggccgtcgg
      121 cgggccggca ttcctcggtc caggccacgc cgccttcggg gcgggcgcgg ggccgggact
      181 cgcaggtctg ttcctggggc ctgacgtcgg ctctcgccgg ccggtcgggc gcgccgcagg
      241 tcagggcgta gcggtcgccg gccgccgcca gccggcattc atccggcgca cccgcgggcg
      301 ccagtcccgg cggcagggcg gcgcccagat tggccgccag cgcgatccgc acccgcgcct
      361 gacaggcctc catcgtctcg gacgccttgc ggatcagtgg actgcattcg gcccgctcat
      421 agccgtacgg atccgcgcgg gcgctgtcgg gaatggcggg gatttcgacc ggcgcagccg
      481 cgacggccgg gggcgccggc tcctcccaca cgacctgacc aatcgcggcg ggcaggggcg
      541 ccgcctgttg cagcatgacg gcgaacacga tggcgagcat ggcggaccct cccggaaact
      601 gccggcagcc taacctattt ggccagcgcc cgcatcgcct tctcgagccc ttccagcgtc
      661 agcggataca tccgctcgtc gaccagttcg cgcaccatcc cgaccgaggc cgaatagctc
      721 caatatcgct cgggcgaggg gttcagccac gccaccttgt cccactgctg cgtggcccgc
      781 ttcatccaga cggcgcccgc ctcctcgttc cagtgctcga ccgagccgcc gggcatggtc
      841 acctcatagg ggctcatggt cgcgtcgccg acgaagatgg cgcgccagtc ggcggggtat
      901 ttgttcagca cctcccaggt cgggatcttc tgctcgtgcc gacgccggtt gtccttccac
      961 accccctcgt agaggcagtt gtggaagtag aagaattcga ggtgtttgaa ctcggtcttc
     1021 gcggccgaga acagctcctc gcacatcttc acatgggcat ccatcgagcc gccgatgtcg
     1081 aggaacagca gcaccttgat ggtgttgcgc cgctctgccc gcatgtgaat gtccagccag
     1141 ccctgacgcg ccgtgccgtc gatggtggcg tccatgtcca gctcgtccgg cgcgccctgc
     1201 cgggcgaacc ggcgcaggcg gcgcagggcc accttgatat tgcgcgtccc cagctcgacc
     1261 tgatcgtcga ggttccggta ctcgcgcttt tcccagacct ttaccgcacg gccctgccgc
     1321 cccggcccgc cgacccgcac cccttccggg ttatagcccc cgtggccgaa cgggctggtc
     1381 ccgccggtgc cgatccattt gttgccgccc tcgtgccggc cctgctgctc ttccagacgt
     1441 tgcttcagcg tctccatcag cttgtcgaac ccgcccagcg cctcgatctg cgccttctcc
     1501 tcgtcggtca gatacttctc gttcagcagc ctcagccagt cctcgggcac atccagcgac
     1561 ggttcgtcgc ccgcgccgac ggtctcgatc ccggcgaaca ccttgccgaa cacctggtcg
     1621 aaccggtcat agtgtttctc gtccttgacc agcaccgccc gcgacaggtg gtagaagtcg
     1681 tcgaccttcc gccccgctac atccttgtcc atggcctcca tcagatggag ccattccttc
     1741 atcgacacgg gcacgcgcgc gtctctcagg gcggtgaaga aggggaggag catcggtgca
     1801 gcctatcagg ccttgtcgac cagcccaatc agctgatcgg cgtctgcggg cgcggcgctt
     1861 ttgatgtcgt tgtcgaagta gacgaagacc tcgcgcttca cccgccaatc ccggatcgcc
     1921 ctcgcccact ccttcagcgc cgcctcgcca tagcgaccat ggtagtggcc gccgggtcca
     1981 tggccgcgca ggtatatgaa gtccgctgtc gcctcccacg gcgcgggcgc atggtggtgg
     2041 tccgagatgc acagggcgac attccgtttg tgcagcatct ccagcacctc gtcggcgtac
     2101 cagcccggat gccggaactc gacgctgtag cgccggccct cgggcagggc gtcgaggaag
     2161 gtctgcagcc gctcgtcgtt gcggtgcaga ttgggcggca gctggaacag gatcggtccg
     2221 atcttgtcgc ccagtccggc gatccgctcg aacatatagg ccaacggctc caccggatcc
     2281 ttcagcttct tgttgtgggt gatgaagcgc gaggccttcc aggcgaatag gaaatcgtcc
     2341 ggcgtctgcg cgcgccagtc cgccaccgcc ttgtctgtcg gcatgcgata gaagctggcg
     2401 ttgatctcca gcgtccggaa ccggcgtgag atatactcca gccgtccggc cgccttcacc
     2461 tcctccggat agaaggggcc gttccagtcc ttgtaggccc agcccgaggt gccgatcctg
     2521 acgtctttca tggtcgtcga accgtcgtcg ctgcacgcgg ttccatccgg tgaagactga
     2581 tgccgccttg cccgaaaaac tcccgaaacg gggccggttt gacgccgatg gatattgggg
     2641 ttccggacag acctctctat tctgccgccg atgcgcaccg ccccgccaga tcgacctgca
     2701 tgacccagcc agacgccacc cttctcgccg ccgtgcagcg gagggactgg gcagccgccc
     2761 gcccggggct cgaggatctg gcggccgccg gtcaggcgga cgcgcgcagc ctcgcatggc
     2821 tggcgacggc tcgggcgcgc gccggtgacg ccgcgctggc gcatgaggct gcggaccggg
     2881 ccctccagtt ggaagcgcgc aatcttcgcg ccgcgctggt gaaggcggac ctgctgtggg
     2941 cggagggcgc caaacgcgag gccaactatt tctacggtgc ggtggtggag atcggcggct
     3001 cggcctccgg ccttgaaccg gacatggtcg cgggcgtcag gcgcgcgcgc acgatcaagg
     3061 acagcctggc ccgcgatatc ttcgcccaca tccgcgacga actgggtcag gcggggtttt
     3121 cggagagccg ctccagcccc cggttcgtcc aggcgttcga cctggcgacc ggccgaaaac
     3181 agccctattt ccagcaaccc aaggccttct atttccccga actgcccaac acccagttct
     3241 atccccgcga ccagttcccc tggctcgaca aggtcgaggc ggcgacggac gccattaccg
     3301 ccgagctcat ggcggtgctc gaagaggacg cggccttcgc gccctatatc cagaccgtcc
     3361 ctaacatgcc gacccggacc gattatccgc tggtcgacag catggactgg agcacctgct
     3421 tcctgtggaa ggacggggcc gaaacgccga acgccgcgcg atgcccgaag accatggcgg
     3481 cgctggaaga cgtcccgctc tgccgcattt cgggccgctc tccacagatc atgttctcgc
     3541 agctcaaggc cggggcgcac atccagcccc accatggcta tgtgaacacc cggctggtct
     3601 gccacctgcc gctgatcgtg ccccccggct gtcgcttccg cgtcggcaat gacgttcgca
     3661 gctgggagaa gggcaaggcc tgggtcttcg acgacacgat cgagcacgag gcc
//
LOCUS       DASVVN010000138         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_83820, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000138 DASVVN010000000
VERSION     DASVVN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83820"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF686_02090"
     CDS             complement(<1..229)
                     /locus_tag="VF686_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HEX8660628.1"
                     /translation="MISGRSVLAVITARGGSKGLPRKNLVPFRGAPLIAWTIRAAQAA
                     PSIDRLILSSDDPEIMETARALGCEAPFRRAP"
     gene            complement(226..>1810)
                     /locus_tag="VF686_02095"
     CDS             complement(226..>1810)
                     /locus_tag="VF686_02095"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX8660629.1"
                     /translation="PEVARVAREAVPPAADRIVLLSEVFKSAPKMSIDYAVMEHSRRI
                     SVLPVDYHWSDLGAWDSVAEGGSGDRGVWIAAEGDSGLVRAADGMVVATAGVTNMAII
                     AERDAILVCSLDRAQEVKGLVERLRDISPAHAKGRWEAGGTLAERARYFDSWMRLNAL
                     PMWAALGVDEDGGFREAIDLSGRPVGDFRRARVQARQAYVYAVAGMAGWAGPWRSLVS
                     RALERFEATNRRTDDLYRTRVSADGAVLDETASLYDQAFALFAFAAAAKAGIAPEMMA
                     ERAHALRGALAGLRLPQGGWREAGDHPYQANAHMHLLEACMAWEAIEPKGEWRAMADE
                     IVALARGAFIDAEGGFLREFFDEEWAPAAGEDGRLIEPGHQFEWAWLLTRWGRLRGEA
                     WALEAAIRLYDVGARGVDQARGVAMDALNDDLSVRSEQARLWPQTERLKATMILAEAA
                     EGGRRILLLDDARKAMAGLQRYLEPSGLWRDKMRADGGFVDEPAPASSLYHIMVAWEQ
                     VRASAGVLPELGGGPAGGS"
BASE COUNT          267 a          718 c          576 g          249 t
ORIGIN      
        1 ccggcgcgcg ccggaacggc gcctcgcagc ccagcgcgcg tgccgtctcc atgatttccg
       61 gatcgtccga cgacagtatc agccggtcga tgctcggcgc ggcttgcgcc gcccgaatgg
      121 tccaggcgat cagcggcgcg ccgcgaaacg gcacaaggtt cttgcgcggc agacccttcg
      181 atccgccccg tgcggtgatg acggccagaa cggagcggcc gctgatcatg atccgcccgc
      241 cggccccccg ccgagctccg gcagaacccc cgccgacgcc ctgacctgct cccaggcgac
      301 catgatgtgg tacagcgagc tggccggcgc gggctcgtcg acgaagcctc cgtcggcccg
      361 catcttgtcg cgccacaggc ccgagggttc caggtagcgc tgcaggcccg ccatcgcctt
      421 gcgcgcatcg tccagcagca ggatccggcg accgccttcg gccgcctccg ccaggatcat
      481 cgtcgccttc agccgctcgg tctggggcca cagccgggcc tgttccgagc gcacggacag
      541 gtcgtcgttc agggcgtcca tggccacgcc gcgcgcctga tccacgccgc gcgcgcccac
      601 gtcgtacagc cggatcgccg cctccagcgc ccatgcctcg ccgcgcaggc ggccccagcg
      661 cgtcagcaac caggcccatt cgaactgatg ccccggctcg atcagccgtc cgtcctcacc
      721 cgccgcgggc gcccattcct cgtcgaagaa ttcgcgcagg aagccgccct ccgcgtcgat
      781 gaacgcgccc cgggccagtg cgacgatctc gtcggccatc gcccgccatt cgcccttcgg
      841 ctcgatggct tcccacgcca tgcaggcctc cagcagatgc atgtgggcgt tggcctgata
      901 cggatgatcg ccagcctcgc gccaaccgcc ctgcggaaga cgcagccccg cgagcgcgcc
      961 ccgcagcgca tgggcgcgtt cggccatcat ctccggggcg atcccggcct tggccgccgc
     1021 cgcgaaggcg aacagagcga aggcctgatc gtaaagggat gccgtttcgt ccagcacggc
     1081 gccgtccgcc gacacccggg tgcggtacag atcgtccgtg cgccggttgg tggcctcgaa
     1141 ccgttccagc gcccggctga ccagagaccg ccacggcccg gcccaacccg ccatcccggc
     1201 aacggcatag acataggcct gacgcgcctg cacccgggcc cggcggaagt cgccgaccgg
     1261 ccgcccggac aggtcgatcg cttctcggaa gccgccgtcc tcgtccacgc ccagggcggc
     1321 ccacatcggc agggcgttca gccgcatcca gctgtcgaaa tagcgcgccc gctcggccag
     1381 tgtcccgccc gcctcccacc gccccttggc atgggccgga gagatgtcgc gcagccgttc
     1441 gaccagcccc ttgacctcct gagcccgatc aaggctgcag accagtatgg cgtcgcgctc
     1501 ggcgatgatg gccatgttgg tcaccccggc ggtggcgacc accatgccgt cggccgcccg
     1561 caccaggccg ctgtcgccct cggccgcgat ccagacgccg cggtcgcccg agcccccctc
     1621 cgcaacggag tcccaggccc ccagatccga ccagtgatag tcgaccggca gcaccgagat
     1681 acggcgactg tgctccatga ccgcgtagtc gatggacatt ttcggagcgc ttttgaacac
     1741 ttcggacagc aggacgatgc ggtccgcggc tggcgggacc gcctcgcgcg cgacgcgggc
     1801 gacctcgggc
//
LOCUS       DASVVN010000139         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_452468,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000139 DASVVN010000000
VERSION     DASVVN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452468"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF686_02100"
     CDS             <1..789
                     /locus_tag="VF686_02100"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX8660630.1"
                     /translation="FQFADGVYEVWSVFDGRLADFDGHMTRLHRSLNELRIPIPMTAE
                     ALGRVLNETVRRNRVNEGLVYLQVTRGTARRDHPFPPEGTPPSVIVTARSLPLSKGNA
                     AARKGVAVVTHPDIRWGRCDIKTVGLLPNVLAKQAAKEKGAAEAWMVDEMGLVTEGSS
                     TNAWIVDENGKLRTRDAQSNILRGITRTAVLKLIEEEGLEFEERAFSVDEAKRAKEAF
                     YTSASGFVMPAVSIDGMKIGNGKPGPIATRLRTLYLDQAIRDAI"
     gene            922..1167
                     /gene="hfq"
                     /locus_tag="VF686_02105"
     CDS             922..1167
                     /gene="hfq"
                     /locus_tag="VF686_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX8660631.1"
                     /translation="MSQDKSKNLQDTFLNSVRKTKTPLTIFLVNGVKLQGIVTWFDNF
                     CVLLRRDGQSQLVYKHAISTIMPSAPVQLYEPEADED"
     gene            1294..>2348
                     /gene="hflX"
                     /locus_tag="VF686_02110"
     CDS             1294..>2348
                     /gene="hflX"
                     /locus_tag="VF686_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8660632.1"
                     /translation="MASQLIDHAVPLIRAIVIHPERSGFGASESSRAAVERLEEAVGL
                     ARALDLDVRAEEIVRIRKPTPATLFGTGKVEELAALVRAAEAEAVVVDDDLSPVQQRN
                     LEKAWDCKVIDRTGLILEIFGRRARTKEGRLQVELARLDYEKSRLVRTWTHLERQRGG
                     TGSTGGPGETQIELDRRLIADRIVKLKLELDEVRRTRGLHRKQRQKVPFPTIALVGYT
                     NAGKSTLFNRLTGSEVFAKDLLFATLDTTQRTIRLPQGRPAIVADTVGFISDLPHELV
                     ESFRATLEEVGEADLILHVRDIASPDTGAQAKDVEAVLKQIPMVEGKARRILEVWNKI
                     DLLDGDAREAVVDHADRL"
BASE COUNT          429 a          795 c          780 g          344 t
ORIGIN      
        1 tttcagttcg ccgacggcgt ctatgaggtc tggtcggtgt tcgacggtcg gctggccgat
       61 ttcgacggcc atatgacgcg cctgcaccgc agcctgaacg agctgcgcat ccctatcccg
      121 atgacggcgg aagcgctggg ccgggtgctg aacgagaccg tgcggcgcaa ccgggtcaat
      181 gaagggctgg tctatctgca ggtcacccgc ggcacggccc ggcgcgatca cccgttcccg
      241 ccggagggca cgccgcccag cgtcatcgtc accgcccgct cgctgccgct gtcgaagggc
      301 aatgcagcgg cgaggaaggg ggtggccgtg gtcacccatc ccgacatccg ctggggccgc
      361 tgcgacatca agacggtcgg cctgttgccc aatgtcctcg ccaaacaggc ggcgaaggag
      421 aagggcgcgg ccgaggcctg gatggtcgac gaaatgggtc tggtcacgga gggctcgtcc
      481 accaacgcct ggatcgtcga cgagaacggc aagctgcgaa cccgcgacgc ccagtccaac
      541 atcctgcgcg gcatcacccg gacggcggtg ctgaagctga tcgaggaaga ggggctggag
      601 ttcgaggaac gggccttcag cgtcgacgag gccaagcgcg cgaaggaggc cttctacacc
      661 tccgccagcg gcttcgtcat gccggcggtg tcgatcgacg ggatgaagat cggaaacggc
      721 aaacccggcc cgatcgcgac gcggctgaga actctgtatc tggaccaggc gatccgcgac
      781 gcgatctgac tcggcccgta tgcacggaaa tgagattgat cggccgccgc cgatgcctat
      841 gctggcgggg ccggacatcc gatccagccc cgcgccgctc ctcctcggcc ggagaacaag
      901 caagaacagg ctgaacctgc catgtcgcaa gacaagtcca agaaccttca ggacaccttt
      961 ctcaacagcg tccgcaagac caagacgccg ctgaccatct tcctggtcaa cggggtcaag
     1021 ctgcagggca tcgtgacctg gttcgacaac ttctgtgtgc tgctgcgccg cgacggccag
     1081 tcgcagctcg tctacaagca cgccatctcg accatcatgc cgtcggcgcc cgtgcagctg
     1141 tacgagcccg aggccgacga ggactgagcc gcttcaaatc cgggcgcggc gcactatctc
     1201 tgcatgcatc cggctgaggc cggatcgtat ggcgtttccg gcggttgatc gtcaggacgc
     1261 ccgttcccga acacgaaagc gaactcacca cacttggcca gccaactgat cgaccacgcc
     1321 gtcccgctga tccgggcgat cgtcatccac cccgagcgtt cgggctttgg cgcaagcgag
     1381 agctcgcgcg cggccgtaga gcgtctggaa gaggccgtcg gcctggcccg ggcgctggac
     1441 ctcgacgtgc gggccgaaga gatcgtgcgc atccgcaagc ccacgcccgc caccctgttc
     1501 gggaccggca aggtcgagga actggccgcg ctcgtgcgcg ccgccgaggc cgaggccgtg
     1561 gtcgtcgacg acgacctgtc gccggtgcag cagcgcaatc tggagaaggc ctgggactgc
     1621 aaggtcatcg accgcaccgg cctgatcctg gagatcttcg gccgccgcgc gcggaccaag
     1681 gaaggccggc tgcaggtcga gctggcgcgg ctggactatg agaagtcgcg gctggttcgg
     1741 acctggaccc accttgagcg gcaacgcggc ggcaccggct cgaccggcgg ccccggcgaa
     1801 acccagatcg agctcgaccg ccgcctgatc gccgaccgga tcgtcaagct gaagctcgag
     1861 ctggacgaag tgcgccgcac gcgcggcctg caccggaaac agcgccagaa ggtcccgttc
     1921 ccgaccatcg ccctggtcgg ctacaccaac gccggcaagt cgacgctgtt caaccggctg
     1981 acgggttccg aggtcttcgc caaggacctg ctgttcgcca ccctggacac gacccagcgc
     2041 accatccgcc tgccgcaggg ccggccggcg atcgtggccg acacggtcgg cttcatttcc
     2101 gacctgccgc acgaactggt cgaaagcttc cgcgccacgc tggaagaggt gggcgaggcc
     2161 gacctgatcc tgcacgtccg cgacatcgcc agccccgaca ccggggccca ggcgaaggac
     2221 gtcgaggcgg tgctgaaaca gatcccgatg gtcgagggca aggcccggcg gattctggag
     2281 gtctggaaca agattgacct gctggacggc gacgctcgcg aggctgttgt tgatcacgcc
     2341 gaccggct
//
LOCUS       DASVVN010000140         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_111133,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000140 DASVVN010000000
VERSION     DASVVN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111133"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VF686_02115"
     CDS             complement(<1..138)
                     /locus_tag="VF686_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX8660633.1"
                     /translation="MFTKVLIANRGEIALRIHRACKEMGISTVAVHSEADRGAMWVRL
                     AD"
     gene            complement(143..655)
                     /gene="accB"
                     /locus_tag="VF686_02120"
     CDS             complement(143..655)
                     /gene="accB"
                     /locus_tag="VF686_02120"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX8660634.1"
                     /translation="MADSKTPAPKLKNDAEIDTTLVRSLADILNDTDLTEIEVERGEL
                     RIRVAREITMAAPVQYAAAPAPAADAPAAPAAAPAAMPSDPATIVARSGEEVKSPMVG
                     TAYLQASPEAPPFCQPGDKVKKGQTLLIVEAMKTMNPIQSPRDGVVKEVLVGDAQPVE
                     FGEPLVVLEA"
     gene            complement(674..1153)
                     /gene="aroQ"
                     /locus_tag="VF686_02125"
     CDS             complement(674..1153)
                     /gene="aroQ"
                     /locus_tag="VF686_02125"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX8660635.1"
                     /translation="MPEPTVLYVLNGPNLNLLGVREPDIYGHESLADVQALCERAADG
                     ATVVFRQTNHEGELVDQVQEAREKASALIINAAGYTHTSVALLDALKTLTIPIVEVHL
                     TNPAARERFRHRSYVSPVAAGVVSGFGSFSYELAVKAALKLVRERSQERAGAAGRSV"
     gene            1253..1450
                     /gene="thiS"
                     /locus_tag="VF686_02130"
     CDS             1253..1450
                     /gene="thiS"
                     /locus_tag="VF686_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX8660636.1"
                     /translation="MRVQVNGELREVAATTILALVEELGLDVRKVAVERNLEIVPRSL
                     HATTALTEGDRVELVQFVGGG"
     gene            complement(1454..>2264)
                     /locus_tag="VF686_02135"
     CDS             complement(1454..>2264)
                     /locus_tag="VF686_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660637.1"
                     /translation="WGWRIMWFLNLPTGLILIALSPLLPESARFLQHIGRGEEARAVL
                     ARFGAGVQTVKATVAPRESHSHLPPVDRRHLAVTIALTLAALSWGLVNFGVLLWLPGA
                     LVAEGRSVEAASTLIAKSTLIAAPTVLISVWLYSAWSTRGSLALMLGVSALGLFGFLL
                     RSMGVEALADPIGPLALLIVGSSGVISIILPYTAENYPLRIRGRATGWVAGCSKLGGL
                     AAQGLSVLGAAPALGVAAAFVGGLAVVALGMIAVLGRETRGRDLRDLEVEP"
BASE COUNT          373 a          710 c          847 g          334 t
ORIGIN      
        1 gtcggcgagg cgcacccaca tggcgccgcg gtccgcttcg gagtgaacgg cgacggtgga
       61 gatgcccatc tccttgcagg cgcggtggat gcgcagggcg atttcgccgc ggttggcgat
      121 caggactttc gtgaacaccg gatcaggcct cgagcacgac gagcggctcg ccgaactcga
      181 ccggctgggc gtcaccgacc aggacttcct tcacgacgcc gtcgcgcgga gactggatcg
      241 ggttcattgt cttcatggcc tcgacgatca gcagggtctg gcccttcttg accttgtcgc
      301 ccggctggca aaacggcggc gcctcgggcg aggcctgcag ataggccgtg ccgaccatcg
      361 gcgacttcac ctcttcgccc gagcgggcga cgatggtggc gggatcggaa ggcatagctg
      421 ccggggccgc agcgggggca gcgggcgcat cggcggcggg agccggggcg gcggcgtact
      481 gcaccggcgc ggccatggtg atctcgcggg cgacccggat gcgcagctcg ccgcgttcga
      541 cctcgatctc ggtcagatcg gtgtcgttga ggatgtcggc cagcgaccgc accagggtgg
      601 tgtcgatctc ggcgtcattc ttgagcttgg gagcgggggt cttggaatcg gccatgtgag
      661 cctctgttgt cctttaaacc gaacggcccg cggccccggc gcgttcctgc gaacgctcac
      721 ggaccagctt gagggcggcc ttgacggcca gttcgtagct gaatgagccg aagcccgaaa
      781 ccacgcccgc cgcgacgggc gaaacatagg agcgatggcg gaagcgttcg cgcgccgccg
      841 gattggtgag atggacctcg acgatcggga tcgtcagcgt cttgagagcg tccagcaagg
      901 ccacggaagt gtgggtgtag ccggccgcgt tgatgatcag ggccgaggcc ttttcgcggg
      961 cttcctggac ctggtcgacc agttcgccct cgtggttcgt ctggcggaag accacggtcg
     1021 cgccgtcggc ggcccgttcg cacagcgcct gaacgtcggc cagcgactca tgcccataga
     1081 tatccggctc gcgcacccca agcaggttga ggttcgggcc gttcaggacg tagaggacgg
     1141 ttggttcggg catgagctcg tggaactgcg ggtccgaccg gaatcggacc ggccttttag
     1201 ctgaggcggg gcggttatca aaccgcggcg ctcaggagag tgacgtaacg gcatgcgcgt
     1261 tcaggtcaac ggagaactcc gcgaggtcgc ggcgaccacc atcctggccc tcgtcgagga
     1321 actggggctg gacgtccgca aggtggcggt cgagcgcaat ctggagatcg tgccccggtc
     1381 gctgcatgcg acgacggcgc tgaccgaagg cgaccgggtc gaactggtcc agttcgtggg
     1441 cggcggctga ggcctagggt tcaacctcga gatcccgcag gtctcggccc cgggtctcgc
     1501 ggcccagcac agcgatcatg ccgagggcga ccacggcaag gccgccgacg aaggccgcgg
     1561 ctacgccgag ggccggggcg gcgcccagaa cgctgaggcc ctgtgcggcc agccccccca
     1621 gcttgctgca gccggcgacc cagcccgtgg cgcggccgcg gatgcgcagg ggatagttct
     1681 cggccgtgta gggcaggatg atcgagatga cgccgctgga gccgacgatc agcagggcga
     1741 ggggcccgat tgggtcggcg agggcctcga cgcccatgct gcgcaacagg aagccgaaca
     1801 ggcccaaggc gctgacgccg agcatcaggg cgagggagcc ccgcgtgctc caggcgctgt
     1861 agagccaaac ggagatcagc acggtgggag ccgcgatcag ggtggatttg gcgatcaggg
     1921 tcgaggcggc ctcgacgctg cggccctcgg ccaccagggc gccgggcagc cagagcagga
     1981 cgccaaaatt gacgaggccc catgacaggg cggccaaggt cagggcgatc gtcacggcca
     2041 gatggcggcg gtcgacgggc ggcaggtgag agtggctttc ccggggtgcg accgtcgcct
     2101 tgacggtctg gaccccggca ccgaaccgcg ccagcacggc gcgcgcctct tcgccacggc
     2161 cgatgtgctg caggaaacgg gcggattcgg gcagcagggg gctgagggcg atcaggatca
     2221 ggcccgtggg caggttgagg aaccacatga tccgccagcc ccag
//
LOCUS       DASVVN010000141         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_57581, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000141 DASVVN010000000
VERSION     DASVVN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57581"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /gene="mraW"
                     /locus_tag="VF686_02140"
     CDS             <1..367
                     /gene="mraW"
                     /locus_tag="VF686_02140"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX8660638.1"
                     /translation="PATRTFQALRIFVNRELEELAEALAAAERVLCEGGRLVVVAFHS
                     LEDRIVKAFLTERTGNAPGGSRHAPVAIETRKPSFTLSFKGAREAGEAELASNPRARS
                     AKLRAAVRTDAAPWGRIAA"
     gene            364..786
                     /locus_tag="VF686_02145"
     CDS             364..786
                     /locus_tag="VF686_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX8660639.1"
                     /translation="MSSAVATLNRLYAWKIRGVRWVEIIGVMLVAAMVFSVYLAKAAA
                     ARESARITDLERQITDNGQRVRLLRAEVARLEQPGRLEALSRGAGLGPVDVHRQATEE
                     SLPTLVAVPEPAPAPVAAAPVEASAPATPAASPAAETQ"
     gene            783..2516
                     /locus_tag="VF686_02150"
     CDS             783..2516
                     /locus_tag="VF686_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8660640.1"
                     /translation="MSAHDPIAGGRPASYGVRRYMPSWRWLAEAMWWVEHSFGRAHAD
                     ARPEEDTRVRIFVILSLFSAVFVCLAVGAAHAALLTEKGGVGFETHPNAVVRADMVDR
                     NGALLATNITHYGLYIDPAEVWDRKAAAAQIKRALPRVTTARLNRVLNGDRRLLVINR
                     LTPQEKVAVHGLALGGVTFEPEDVRVYPLGQSARHVVGNVDTGGVGLSAAERAFNDEI
                     RAAGARGETFQISIDLRIQGILENELAAAAAESQAKGAVGIITDVQTGEVLGMASWPG
                     VNSRNLASAATYEMGSVFKTFTVAAAIDTGRADMNTLLDASEALQIGKRRITDFHATN
                     KVLTLEEVYLHSSNIGTSRLSTEMGPRVMRDYFQRFGLMSRAPFEIASETPIIPREWS
                     DSTLASLSFGYGISITPLQMAAAMQALTNGGRYIPLTLRKGGRPDVQARQVISPETSL
                     TMLDLMRRNVVRGSGGRAEAPGLRVGGKTGSANKSVNGRYSTAHAVGTFAGVFPADGP
                     VNARRYQILVLIDEPQTYPRTGGFVAAPAVGRIADRIAPFLNVQRRADPYRTAVGDKI
                     PAFEDLEGDGR"
     gene            2513..>2674
                     /locus_tag="VF686_02155"
     CDS             2513..>2674
                     /locus_tag="VF686_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX8660641.1"
                     /translation="MSAAPAPVKLSELLRRDVAADPVITGVTADSRKVAPGSLFVALP
                     GSAADGRAFI"
BASE COUNT          378 a          973 c          923 g          400 t
ORIGIN      
        1 tcccgcgacg cgcacctttc aagcgctccg tatcttcgtg aaccgcgagc tggaggagct
       61 ggccgaggcg ctcgccgcag ccgagcgtgt tctctgcgag ggtggccgcc tcgtcgtcgt
      121 cgccttccat tcgctggagg accggatcgt caaggcgttc ctgaccgagc ggacgggcaa
      181 tgcgccgggc ggctctcgcc atgcgcccgt cgccatcgag acccgcaagc ccagcttcac
      241 cctgtcgttc aagggcgcgc gcgaggcggg cgaggccgag ctggcgtcca atccccgcgc
      301 ccggtcggcc aagctgcgcg ccgccgtccg caccgacgcc gcgccgtggg ggaggatcgc
      361 cgcatgagct cggccgtcgc caccctgaac cggctctacg cctggaagat ccgcggcgtg
      421 cgctgggtcg agatcatcgg cgtgatgctg gtcgcggcca tggtgttctc ggtctatctg
      481 gccaaggccg ctgccgcccg cgagagcgcc cgcatcaccg atctggagcg ccagatcacg
      541 gacaacggcc agcgggtccg tctgctgcgc gccgaggtgg cgcggctgga gcagcccggc
      601 cggctggagg ccctgtcgcg cggcgccggt ctggggccgg tcgatgtgca ccgtcaggcg
      661 accgaagaaa gcctgccgac cctggtcgcc gtgcctgagc cggcccctgc tccggtcgcg
      721 gcggcgcccg ttgaggcctc cgcgcctgca acccccgcgg cttcgcctgc agcggagacc
      781 caatgagcgc gcacgatccg atcgccggag ggcgtcccgc gtcgtacggc gtgcggcgct
      841 acatgccctc gtggcgctgg ctggccgagg ccatgtggtg ggtcgagcat tcgttcggcc
      901 gcgcccatgc cgacgcccgg cccgaggaag acacccgggt ccgcatcttc gtcatcctgt
      961 cgctgttctc ggcggtgttc gtctgtctgg cggtgggcgc ggcccatgcc gccctgctga
     1021 ccgaaaaagg cggtgtcggg ttcgagaccc atccgaacgc cgtggtccgc gccgacatgg
     1081 tcgaccgcaa cggcgccctg ctggccacca acatcaccca ctacggcctc tatatcgatc
     1141 cggccgaggt ctgggatcgc aaggccgcgg cggcgcagat caagcgcgcc ctgccgcgcg
     1201 tgacgacggc caggctgaac cgcgtgctga acggcgaccg gcggctgctg gtcatcaacc
     1261 gcctgacccc gcaggagaag gtggcggtcc atggtctggc cctgggcggc gtgacgttcg
     1321 agcccgagga cgtgcgggtc tatccgctgg gccagtcggc gcgccatgtg gtcggcaatg
     1381 tcgacaccgg cggcgtcggc ctgtccgcgg ccgaacgcgc cttcaacgac gagatccggg
     1441 cggccggcgc gcgcggcgag accttccaga tctccatcga cctgcgcatc cagggcattc
     1501 tggagaacga gctcgccgcc gccgccgccg agagccaggc caagggcgcg gtcggcatca
     1561 tcaccgacgt ccagaccggc gaggttctgg gcatggccag ctggccgggc gtgaactcgc
     1621 gcaacctggc ctctgccgcg acctatgaga tggggtcggt gttcaagacc ttcaccgtcg
     1681 ctgccgccat cgacaccggc cgggccgaca tgaacaccct gctggacgcc tcggaggcgc
     1741 tgcagatcgg caagcggcgg atcaccgact tccacgccac caacaaggtg ctgacgctgg
     1801 aggaggtcta tctgcactcg tccaacatcg gcacctcgcg cctgtcgacc gagatggggc
     1861 cgcgggtgat gcgcgactat ttccagcgct tcggcctgat gtcgcgggcg ccgttcgaga
     1921 tcgcgtcaga gacgccgatc atcccgcgcg agtggtcgga ctcgaccttg gcctcgctgt
     1981 cgttcggcta tggcatctcg atcacgccgc tgcagatggc ggcggcgatg caggccctga
     2041 ccaatggcgg gcgctacatc ccccttaccc tgcgcaaggg cggaaggccc gacgtgcagg
     2101 cgcggcaggt gatctcgccc gagacctccc tgacgatgct ggacctgatg cgacgcaacg
     2161 tggtgcgcgg ttcaggcggc cgggccgagg ctccgggcct gcgcgtcggc ggcaagacgg
     2221 gctcggccaa caagtccgtc aacggccgct attcgacggc ccacgcggtc ggcaccttcg
     2281 ccggcgtctt ccccgcagac ggcccggtca atgcgcgccg ctatcagatt ctcgttctga
     2341 tcgacgagcc ccagacctat ccgcgcaccg gcggcttcgt ggccgcgccg gcggtgggcc
     2401 ggatcgcaga ccggatcgcg ccgttcctga acgtccagcg ccgcgccgac ccctaccgca
     2461 ccgccgtcgg cgacaagatt ccggccttcg aagaccttga aggcgacggc cgatgagcgc
     2521 cgcccccgcc ccggtcaaac tgtctgaact gctgcgccgc gacgtcgccg cggacccggt
     2581 catcaccggc gtgacggcgg acagccgcaa ggtcgcgccc gggtcgctgt tcgtggccct
     2641 gccgggcagc gccgccgacg ggcgcgcctt catc
//
LOCUS       DASVVN010000142         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_57593, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000142 DASVVN010000000
VERSION     DASVVN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57593"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VF686_02160"
     CDS             complement(<1..439)
                     /locus_tag="VF686_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8660642.1"
                     /translation="MPGLIRRCRLPASQQPTKRVMNDRSAEDRHPLDRLSPGERQVLI
                     LLARGHTAKTIATSLGVSVGAVNERLRSARRRTGAGSSRELARLLAQENRDEFSGVAA
                     NDGSPSGLGDPAPKGRWNTRRNVMLASAALAAAVAIILAPQIAG"
     gene            589..>2157
                     /locus_tag="VF686_02165"
     CDS             589..>2157
                     /locus_tag="VF686_02165"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX8660643.1"
                     /translation="MLHWLVIIVLAVVVYRQGEALKRLEALLGKALGSRDALPDPVRP
                     AEPPLASAALKPASVAEVRPAARVSTPVTPTPSPTPPPRVTEFTRQPVPPQRPPEPRP
                     APKPCPAPASNPVKWASVSTWLAENGLAWIGGGGLALGGLLLVVYAAQQGVFTPPLRI
                     AAAVGLGGMMIAASEWILRQKQAPGGRHLLAAAVAAGAGAVTLYGAVCAAYSLYGLIS
                     FPVAAVLTGAISLGLLGLSLRHGEPLALLAVFGAVITPWVTGISVWSPTVLLAYALLI
                     GVTGFVISAARLWGKAGFLTLGGLLVWSLSEAFGGGAAALLLLAAAGPACAVFWRRAN
                     RQDDPADPHAKAFFHLPTAGLVFASLLSARLWTGDPGSVAHLPGAVLISGALVALGAA
                     MVVLRLAPAGVFATPVAVAVFATLATLGLDGRHPAFGAVLPWIHGLTALIPVAALFAA
                     LRSPPAARTILLGVGGIGVAVLANLAWPLLDAAGIQLAWLPAAILSAALFATALLIAR
                     RVEDVQRDRGLVLWL"
BASE COUNT          232 a          829 c          742 g          354 t
ORIGIN      
        1 ggccggcgat ctggggcgcc aggatgatgg cgacggcggc ggcgagggcg gcgcttgcga
       61 gcatgacgtt tctccgggtg ttccagcgcc ccttcggcgc cgggtctccc aaaccggacg
      121 gcgacccgtc gtttgcggca accccggaaa actcgtcacg attttcctgg gccagaagcc
      181 gggccagttc gcggctggac ccggccccgg tccggcgccg ggcgctgcga agccgctcgt
      241 tcacggcgcc gaccgaaacc ccgaggcttg tggcgatcgt cttggcggtg tggccccgcg
      301 cgagcaggat caggacctgc cgttcgccgg gactgaggcg atcaagtgga tggcgatctt
      361 cggcactccg gtcgttcatc acgcgcttcg tcggctgttg ggacgccggc agcctgcacc
      421 gccggatgag gccgggcaat cgcgacccgc ccgtcgcggc atacaagaaa atcgtccacg
      481 gcggccgggg ccagccatgg cctgtcgcgc ttaaggtgtt gtaccacaat ccacgatagc
      541 cgtggcgggt gaccggggcg tagggtcgcg cgtgtcgagg ggaccgccat gctgcattgg
      601 cttgttatca tcgtgctggc ggtggtcgtc taccgtcagg gcgaagcgct caaacggctc
      661 gaggccctgc ttggcaaggc gctcggttcc cgcgacgccc tgcccgaccc ggtccgtccc
      721 gcggagccgc ccctggcgag cgccgccctg aaaccggcga gcgtcgccga agttcgcccg
      781 gctgcgagag tcagcacgcc ggtgaccccc acgccgtctc cgactccacc gccccgggtg
      841 accgaattca ctcgccaacc cgtcccgccg cagcggccgc cggaacctcg ccccgcgccc
      901 aagccgtgcc cggccccagc gtcgaacccg gtgaagtggg cctcggtctc gacctggctg
      961 gcggagaacg gcctggcctg gatcggcggc ggcggtctgg cgctcggcgg cctgctgctg
     1021 gtcgtctacg ccgcccagca gggcgtcttc accccacccc tgcgcatcgc cgcggccgtc
     1081 ggactgggcg gcatgatgat cgccgccagc gaatggatcc tgcgccagaa acaggcgccg
     1141 ggcggacggc atctgctggc cgccgccgtc gccgccggcg cgggcgcggt caccctgtat
     1201 ggcgcggtct gcgccgccta ttccctgtac ggcctgatct ccttcccggt cgccgccgtg
     1261 ctgacgggcg ccatctccct cggtctgctc ggcctttcgc tccgccacgg cgagcccctc
     1321 gccctgctgg ccgtcttcgg ggccgtgatc acgccctggg tcaccggcat atcggtctgg
     1381 tcgcccaccg tccttctggc ctacgccctt ctcatcggcg tcaccggctt cgtcatcagc
     1441 gccgcccggc tgtggggcaa ggccggtttc ctgaccctgg gcggtctgct ggtctggagc
     1501 ctgtccgagg cgttcggcgg cggcgcggcc gcgctgctgc ttctggccgc ggccggcccg
     1561 gcctgcgccg tgttctggcg gcgcgccaac cggcaggacg acccggccga cccgcacgcg
     1621 aaagccttct tccacctgcc caccgccgga ctggttttcg ccagcctgct ctccgctcgg
     1681 ctctggaccg gcgacccggg cagcgtcgcc catctgccgg gcgcggtcct gatctcgggc
     1741 gcgctcgtgg cgctgggggc cgcgatggtc gtgctgcgcc tcgcccccgc gggagtcttc
     1801 gcgacgcccg tggccgtggc cgtgttcgcc accctcgcga ccctcgggct ggacggacgc
     1861 catcccgcct tcggcgccgt tctgccgtgg atccacggcc tgaccgccct gatccccgtc
     1921 gcggccctct tcgcggccct gcgttcgccg ccggctgcgc gcaccatcct gctgggcgtc
     1981 ggcggtatcg gcgtcgccgt cctcgccaac ctcgcctggc cgctgctgga tgcggccgga
     2041 attcagctgg cgtggctgcc ggcggccatc ctcagcgccg ccctgttcgc aaccgccctc
     2101 ctgatcgccc gccgggtcga ggacgtccag cgcgaccggg ggctggtcct gtggctc
//
LOCUS       DASVVN010000143         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_136602,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000143 DASVVN010000000
VERSION     DASVVN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136602"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VF686_02170"
     CDS             <1..371
                     /locus_tag="VF686_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660644.1"
                     /translation="AAAPPPAAVEPPAQVGSLAGAWRVAGIDGHSFDEPVGLSLTGDA
                     QQLWWEPRCAGVARAYRIEGQRITFASQGAGGPVCDIGQPPRLEEVVRALDGADSISR
                     TPNNGVLISGPEHSVLLFSQ"
     gene            complement(921..1535)
                     /locus_tag="VF686_02175"
     CDS             complement(921..1535)
                     /locus_tag="VF686_02175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8660645.1"
                     /translation="MTRHKIAIVVGSLRKDSINRKIARSICAFSADKLDCNLVEIGDL
                     PLYDQDSDAGPPPPQFARFRQEIAAADGILFCTPEYNRGVPGVLKNAIDVGSRPWGQS
                     VWDKKPAAIVSASPGAIGGFGANHQLRQACVFLNMPVMQQPEAYLGNVGDDQFDDDGC
                     LRDGPLKDVVMGLAGAFADWVDIIARSRRLLAEDAAHAAKTGER"
     gene            complement(1650..2369)
                     /locus_tag="VF686_02180"
     CDS             complement(1650..2369)
                     /locus_tag="VF686_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8660646.1"
                     /translation="MIERHLMKLRARTPLSADEEATIRAAVSGTRRLKPDEILVRAFE
                     RVEVSSILVSGFAARVRQLADGRRQTSELHTPGDFADLHSFTLKYLDHDIVAMTECEF
                     AIVPHERLHAITEQHPRLTRIYWFATNLDAAIHRELVVSLGRREATSRMAHLFCELHI
                     RLGLVGLVENDAFDLPLTQAEMAECLGLSSVHVNRTLMQLRASGLVEWTGKRVSIHDL
                     DGLRRTADFDPGYLHLDKIPR"
     gene            complement(2366..2617)
                     /locus_tag="VF686_02185"
     CDS             complement(2366..2617)
                     /locus_tag="VF686_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660647.1"
                     /translation="MPKFYFHLINQVSARDHEGRELADLDEATAAARADILSILGDDA
                     RRGRLDLRGRIDIADETGAIVRTVAFADAVQLHLPGDAE"
     gene            2771..>3598
                     /locus_tag="VF686_02190"
     CDS             2771..>3598
                     /locus_tag="VF686_02190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8660648.1"
                     /translation="MTALPDIHGVCPPRFAAVKDAFAVNFTDAPEGLNERGARVSVCI
                     GGEAVIDLWGGWADTAQTVPYDGTTLTPVFSAGKAVMSLLIATCVERGLLDYERPVSD
                     WWPAFGQAGKAAITVGQMMSHQAGLPGFDTPEDPAIWFDREATLARLCAQAPMWPPGT
                     ASGYSPVVIGYLAGELFRLVDGRTLGTGLREDFAGRHGLDLWIGLPQSEHGRVAQMRK
                     PGKAADLGPVDAIKTAAFLDRGSAPGRDSAEWRMAEIPSANLHGNARDLARIVGVFVD
                     "
BASE COUNT          543 a         1150 c         1320 g          585 t
ORIGIN      
        1 ctgcggccgc gccgcccccg gcggcggttg aaccgccggc gcaggtcggg agcctggcgg
       61 gggcgtggcg ggtcgcgggg atcgacggcc actcgttcga cgagccggtc gggctgtcgc
      121 tgacgggcga cgcacagcaa ctgtggtggg agccgcgatg cgccggggtg gcgcgcgcct
      181 accgcatcga agggcagcgg atcacgttcg cctcgcaggg agcgggcgga cccgtgtgcg
      241 atatcggcca gccgccccgt ctggaagagg tggtccgcgc gctggacggc gccgacagta
      301 tttcgcggac tcccaacaac ggcgtgctga tctccggccc ggagcacagc gttctgctgt
      361 tctcccagta gtccgcatgg cggctaccgg ccctcaggct tcctgcgatc gcgcacctgc
      421 atctggttga ggctgccgcc gcccaccagc atcgccgggt cgcgacgggc ccgacgacgc
      481 ggcttcagcc acaggcttgc ggccagtcct gcaaggcaaa ccaccaacag agatgcaaag
      541 atcgaatacg ccggagtcca ctgaaccatt ataagtcgct ttgagccgca cctgtcggat
      601 tggcgggcgt gcccggatac cccggcacgc tcacggccgg cccgctccgc ggcggaacca
      661 ggccatgaac agcctgtaca gccagcctcc gcgcgctttc tcgacacgga ccttcgcctg
      721 ccgcccacgg cccttgtagt cgttcaccaa catcgaacac cccgccagaa accgcgcttt
      781 tccggatgtc tcaatccggc acgagctttg cggttcctgt gtccgggtgc ggacagaagt
      841 tgactgcccc cgaatctgat ctctggccca cggcggcgtg gaaacggcaa gcaggatggg
      901 cggcgttccg acagtcgcgc ctagcgttcc cccgtcttcg cggcatgggc cgcgtcttcg
      961 gccagaagcc ggcggctgcg ggcgatgatg tcgacccagt cggcgaaggc gccggccaga
     1021 cccatgacga cgtccttcag cgggccgtcg cgaaggcagc cgtcgtcgtc gaactgatcg
     1081 tctccgacgt tcccgagata ggcctcgggc tgctgcatca ccggcatgtt cagaaacacg
     1141 caggcctgac gcagctggtg gttggcgccg aagccgccga tggcgcccgg cgaggcgctg
     1201 acgatggcgg cgggcttctt gtcccagacg ctctggcccc agggccggga gccgacgtct
     1261 atggcgttct tgagcacgcc ggggacgccg cgattgtatt cgggggtgca gaacaggatg
     1321 ccgtctgcgg cggcgatctc ctgccggaag cgcgcgaact gcggcggcgg cgggccggcg
     1381 tcgctgtcct gatcgtagag gggcaggtcg ccgatttcga ccagattgca gtccagcttg
     1441 tccgccgaga aggcgcagat cgagcgcgcg atcttgcggt tgatactgtc cttgcgaagg
     1501 ctgccgacga cgattgcgat cttgtgtctg gtcatgcgga ggtcccttcg tggcgaggat
     1561 cgaggcgcgg gcgcccgacg cagatcgggt ctggcccgcc ctctcggtga agtcctgacc
     1621 gggggctgcg ttccccggtc acgcctcggt cagcggggaa tcttatcgag gtggagatag
     1681 ccgggatcga agtcggcggt ccggcgcagg ccatcgaggt cgtggatgga gacccgttta
     1741 ccggtccatt ccaccaggcc cgacgcgcga agctgcatca gcgtgcggtt cacatgcacg
     1801 ctcgacaggc cgaggcattc ggccatttcc gcctgcgtca ggggcaggtc aaaggcgtcg
     1861 ttctcgacca ggccgaccag accgagacgg atgtggagct cgcagaagag atgcgccatc
     1921 cggctggtcg cctcgcgccg gcccagggag acgaccagtt cgcggtgaat ggccgcgtcg
     1981 aggttggtgg cgaaccagta gatacgggtc aggcgcgggt gctgttcggt gatggcgtgc
     2041 agccgctcgt gcggcacgat ggcgaactcg cactccgtca tcgccacgat gtcgtggtcg
     2101 agatatttga gcgtgaagct gtggaggtcg gcgaagtcgc ccggcgtatg cagttcggag
     2161 gtctggcgcc gtccgtcggc cagctgtcga acgcgggcgg cgaagccgga cacgaggatc
     2221 gaactgacct cgacccgctc gaaggcgcgg acgaggatct cgtccggttt caggcgccgg
     2281 gttccagaga cagcggcgcg gatggtcgcc tcttcatcgg cgctgagcgg ggtgcgcgcc
     2341 cgcagcttca tcaggtgccg ctcgatcatt cggcatcgcc cggcagatgg agttgcaccg
     2401 cgtcggcgaa ggcgacggtg cgtacgatgg cccccgtctc gtccgcgatg tcgatccggc
     2461 cgcgcaggtc caggcggccg cggcgggcgt cgtctccgag gatcgacagg atgtcggccc
     2521 gcgccgccgc tgtcgcttcg tcgagatcgg cgagctcccg cccttcgtga tcccgcgccg
     2581 aaacctgatt gatgagatga aaatagaatt tgggcatcgg cgcggggctg tggcggagac
     2641 cctgtcgctc ctacccgtcg ggtgcgggcg cgccttaaca tatgttggac cgatggaccg
     2701 ggaagaggat gttggcccaa ggttcgatgc ggcaggtgac ggggccgccg ccggcggtta
     2761 ggacagccgc atgaccgccc tccccgatat ccacggcgtc tgcccgccgc gcttcgccgc
     2821 cgtgaaggac gccttcgccg tcaacttcac cgacgcgccc gaggggctga acgagcgggg
     2881 ggcgagggtc tcggtgtgca tcggcggcga ggccgtgatc gatctgtggg gcggatgggc
     2941 cgatacggcg cagacggttc cctatgacgg gacgacgctg acgccggtgt tctcggcggg
     3001 caaggcggtg atgtcgctgc tgatcgcgac atgcgtcgag cgggggctgc tggattacga
     3061 gcggccggtt tcggactggt ggccggcctt cggacaggcg ggcaaggcgg cgatcacggt
     3121 cgggcagatg atgagccatc aggcgggcct gccgggcttc gacacgcccg aggacccggc
     3181 catctggttc gaccgggagg cgaccctggc ccggctgtgc gcccaggcgc cgatgtggcc
     3241 gccggggacg gcgtcgggct acagcccggt ggtcatcggc tatctggcgg gcgagctgtt
     3301 ccggctggtg gacgggcgga ccttggggac ggggctgcgc gaggatttcg ccgggcggca
     3361 cggtctggac ctgtggatcg gcctgccgca gagcgagcac ggccgggtgg cgcagatgcg
     3421 caaacccggc aaggcggcgg atctgggacc cgtggacgcg atcaagacgg cggccttcct
     3481 cgaccggggg tcggcgccgg ggcgggattc agccgaatgg cggatggccg agattccgtc
     3541 ggccaatctg catggcaatg cccgcgatct ggcccggatc gtcggggtgt tcgtcgac
//
LOCUS       DASVVN010000144         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_188600,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000144 DASVVN010000000
VERSION     DASVVN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188600"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..389)
                     /locus_tag="VF686_02195"
     CDS             complement(120..389)
                     /locus_tag="VF686_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX8660649.1"
                     /translation="MAGKLQFSLVSPEREVFNGLVDQVDAPGAEGDFGVLADHAPFMT
                     ALREGVVTVIDGGSRRQFEVHGGFADVTPAGLTILAEQASEVAAA"
     gene            complement(389..1939)
                     /gene="atpD"
                     /locus_tag="VF686_02200"
     CDS             complement(389..1939)
                     /gene="atpD"
                     /locus_tag="VF686_02200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX8660650.1"
                     /translation="MTDTTAPKKPAARKAAPKAPAAAAATGNAAYTAGTGVGKIAQVI
                     GAVVDVEFEGQLPAILNALETTNVDQKTGKPFRLVLEVAQHLGENMVRAIAMDTTEGL
                     TRGQAVTDTGTSILAPVGPGTLGRIMNVVGDPIDEQGAIKTQMYRPIHREAPSFEEQS
                     TSSEILVTGIKVIDLICPYTKGGKIGLFGGAGVGKTVTMQELINNIAKAYGGYSVLAG
                     VGERTREGNDLYHEMIESNVNVDPAKNGGSTEGSKCALVYGQMNEPPGARARVALTGL
                     AQAEYFRDEEGKDVLLFVDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATEMGNLQER
                     ITSTKKGSITSVQAIYVPADDLTDPAPAASFAHLDATTVLSRDIAAQAIFPAVDPLDS
                     TSRIMDPLVIGDEHYQVARSVQEVLQQYKALKDIIAILGMDELSEEDKLVVSRARKIQ
                     RFLSQPFFVAEQFTNSPGKFVELADTIRSFKGIVAGEYDHLPEAAFYMVGAVEEAVEK
                     AAKMAAEV"
     gene            complement(1977..2855)
                     /locus_tag="VF686_02205"
     CDS             complement(1977..2855)
                     /locus_tag="VF686_02205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX8660651.1"
                     /translation="MASLKEMRNRIGSVKATQKITKAMQMVAAAKLKRAQDQAVAARP
                     YAQRMAAVIANLAAGVSGDGAPKLLAGTGSDKKHLVIVATADKGLAGGFSSNVVRAAR
                     DRINSLISEGKDVRIIAVGRKSRDGLTRMFGDRVVKTFELSDHKVLGLPAAQPIAELV
                     ANEFEAGNADVVTLFYSQFKSVISQVPTAKQLVPAVVEGGAAPINLNGAVYDYEPSEE
                     EILETLLPRNITTQILASMYENQAGFYGAQMGAMDSATRNAGDMISALTLTYNRSRQA
                     QITKELIEIISGAEAL"
     gene            complement(2882..>4182)
                     /gene="atpA"
                     /locus_tag="VF686_02210"
     CDS             complement(2882..>4182)
                     /gene="atpA"
                     /locus_tag="VF686_02210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX8660652.1"
                     /translation="DNQIAEGDEVRRLGEIVDVPVGKGLLGRVVNPLGEPIDGKGPIQ
                     FTERRRVDVKAPGIIPRKSVHEPMQTGLKAIDTLIPIGRGQRELIIGDRQVGKTAVAI
                     DTILNQKSTNAAATSEGEKLYCIYVAIGQKRSTVAQIVKTLEERGALDYTIVVAATAS
                     EPAPLQFLAPFSGTAMGEFFRDNGMHALIVYDDLSKQAVAYRQMSLLLRRPPGREAYP
                     GDVFYLHSRLLERSAKLNEDNGSGSMTALPLIETQANDVSAYIPTNVISITDGQIFLE
                     SDLFYQGIRPAVNVGISVSRVGSSAQIKAMKQVAGSIKGELAQYREMAAFAKFGSDLD
                     ASTQKLLARGARLTELLKQPQYSPLTVEEQVVSVYAGTRGYLDNVAVSDIGRFESELL
                     ARIHSKHSSIMDGIRKEKALSKDLEAELKAAIEAFAKTFA"
BASE COUNT          626 a         1283 c         1503 g          770 t
ORIGIN      
        1 cgttgaagac gccgggccgt cccggacttc aaccccgtcc ggagacaggg gaggacgtgg
       61 cctcgatcaa caatgtggga cagggcggcg gcggttcaag ccgcgcccgt taccggatat
      121 caggcggcgg ccacttccga ggcctgttcg gcaaggatgg tcaggccggc cggggtcacg
      181 tcggcgaagc cgccgtggac ctcgaactgg cggcgcgaac cgccgtcgat gaccgtgacc
      241 acgccttcgc gcagggcggt catgaagggg gcgtggtcgg ccagaacgcc gaagtcgccc
      301 tcggcgccgg gcgcgtcgac ctgatcgacg aggccgttga agacctcgcg ctcaggcgac
      361 accagcgaga actggagctt tccggccatc agacttcggc agccatcttg gcggccttct
      421 cgacggcttc ctcgacggcg ccgaccatgt agaaggcggc ttccggcagg tggtcgtatt
      481 caccggcgac gatgcccttg aacgagcgga tcgtgtcggc cagctcgacg aacttgccgg
      541 gcgagttggt gaactgctcg gccacgaaga agggctgcga caggaagcgc tggatcttgc
      601 gggcgcgcga gacgaccagc ttgtcctctt ccgacagctc gtccatgccc aggatggcga
      661 tgatgtcctt cagggccttg tactgctgca ggacttcctg aaccgaacgg gcgacctggt
      721 agtgttcgtc gccgatgacc agcgggtcca tgatccgcga ggtggagtcc agcggatcca
      781 cggcggggaa gatggcctgg gcggcgatgt cgcggctcag cacggtcgtc gcgtcaaggt
      841 gggcgaacga ggcggcgggg gccgggtcgg tcaggtcgtc ggcgggaacg tagatggcct
      901 ggaccgaggt gatcgagccc ttcttggtcg aggtgatgcg ctcctgcagg ttgcccatct
      961 cggtggccag cgtcggctga tagcccacgg ccgaggggat gcggcccagc agggcggaca
     1021 cttccgagcc cgcttgcgtg aagcggaaga tgttgtcgac gaacagcagc acgtccttgc
     1081 cttcctcgtc gcggaagtac tcggcctggg ccagtccggt cagggcgacg cgggcgcgcg
     1141 cgccgggagg ctcgttcatc tggccgtaga cgagggcgca tttcgagcct tcggtcgagc
     1201 cgccgttctt ggccgggtcc acgttcacgt tggactcgat catctcgtga tacaggtcgt
     1261 tgccctcgcg ggtgcgctca cccacgccag ccagaaccga ataaccgccg taggccttgg
     1321 cgatgttgtt gatcagctcc tgcatggtca cggtcttgcc cacgccggcg ccgccgaaca
     1381 ggccgatctt gccgcccttg gtgtaggggc agatcaggtc gatgaccttg atccccgtga
     1441 cgaggatttc cgacgacgtc gactgctctt cgaacgaagg cgcctcgcgg tggatcgggc
     1501 ggtacatctg ggtcttgatc gcgccctgtt cgtcgatcgg atcgccgacg acgttcatga
     1561 tgcggcccaa ggtgccgggg ccgacggggg ccaggatgga ggtgccggtg tcggtcacgg
     1621 cctggccgcg ggtcagaccc tcggtggtgt ccatggcgat ggcgcggacc atgttctcac
     1681 ccaggtgctg ggcgacttcc agaaccagac ggaagggctt gccggtcttc tgatcgacgt
     1741 tggtcgtttc cagcgcgttc aggatggccg gcagctgacc ttcgaactcg acatcgacga
     1801 cggcgccgat gacctgggcg atcttgccca cgccggtgcc ggccgtatag gcggcgttac
     1861 cggtggcggc ggcggctgcg ggagccttgg gagcggcctt gcgggcggcg ggcttcttgg
     1921 gggcggtggt gtcggtcatc agtgcgttcc ggttcggaat gtcggtcagg atcggatcag
     1981 agggcttcgg cgccggagat gatctcgatc agctccttgg tgatctgcgc ctggcgggat
     2041 cggttgtagg tcagggtcag ggcggaaatc atgtcgcccg cgttgcgcgt cgcggagtcc
     2101 atggcgccca tctgggcccc gtagaagccg gcttggttct cgtacatcga ggccaggatc
     2161 tgggtggtga tgttgcgcgg cagcagggtc tcgaggattt cctcctcgga aggctcgtag
     2221 tcgtagacgg cgccgttcag gttgatggga gcggcgcccc cctcgaccac ggcggggacc
     2281 agttgtttgg cggtcgggac ctgcgagatc accgacttga actggctgta gaacagggtg
     2341 acgacgtcgg cgtttccggc ctcgaactca ttggccacca gctcggcgat cggctgggcg
     2401 gcgggcaggc cgaggacctt gtggtccgac agctcgaacg tcttgacgac gcggtcgccg
     2461 aacatccggg tcaggccgtc gcgggacttg cggcccacgg cgatgatgcg gacgtccttg
     2521 ccctcggaaa tcaggctgtt gatccggtcg cgggcggcgc ggacgacatt ggacgagaag
     2581 ccgccggcca ggcctttatc ggccgtggcg acgatgacga ggtgcttctt gtccgagccc
     2641 gtgccggcca gcagtttggg cgcgccgtcg ccggacacgc cggccgccag attggcgatg
     2701 acggcggcca tgcgctgggc gtagggccgg gccgcgacgg cctggtcctg ggcgcgtttc
     2761 agcttggccg cggcgaccat ctgcatggct ttcgtgatct tctgcgtggc tttcacgctt
     2821 ccgatccgat tgcgcatttc cttgaggctg gccatccggc gctactctct cgctctcggt
     2881 cttacgcgaa ggtcttggcg aaggcctcga tggcggcctt cagctcggct tccagatcct
     2941 tggacagggc cttctccttg cggatgccgt ccatgatgga gctgtgcttg gagtggatgc
     3001 gggccagcag ctcgctctcg aagcgtccga tgtcggacac cgcgacgttg tccagatagc
     3061 cgcgcgtgcc ggcgtagacc gaaaccacct gctcctcgac cgtcagcggc gagtactggg
     3121 gctgtttcag cagttccgtc aggcgggcgc cgcgggccag aagcttctgg gtcgaggcgt
     3181 cgaggtccga gccgaacttg gcgaaggcgg ccatctcgcg atactgggcg agctcgccct
     3241 tgatggagcc ggcgacctgt ttcatcgcct tgatctgggc cgaggagccc acgcgcgaca
     3301 ccgagatgcc gacgttcacg gcggggcgga tgccctggta gaacaggtcc gattccagga
     3361 agatctggcc gtcggtgatc gaaatcacgt tggtcgggat gtaggccgag acgtcgttgg
     3421 cctgggtctc gatcagcggc agggcggtca tcgagcccga accgttgtcc tcgttcagct
     3481 tggccgaacg ctccagcagg cggctgtgca ggtagaagac gtcgcccgga taggcttcgc
     3541 ggcccggcgg acggcgcagc agcagggaca tctggcgata ggccacggcc tgcttcgaca
     3601 gatcgtcata gacgatcagc gcgtgcatgc cgttgtcgcg gaagaactcg cccatggccg
     3661 tgcccgagaa cggggccagg aactgcagcg gggccggctc cgacgccgtg gcggcgacga
     3721 cgatggtgta gtccagggcg ccgcgctcct cgagcgtctt gacgatctgg gcgacggtcg
     3781 agcgcttctg gccgatggcg acgtagatgc agtacagctt ctcgccttcc gacgtggcgg
     3841 cggcgttggt gctcttctgg ttcaggatgg tgtcgatggc gacggcggtc ttgccgacct
     3901 gacggtcgcc aatgatcagc tcgcgctggc cgcggccgat cgggatcagg gtgtcgatgg
     3961 ccttcagacc ggtctgcatg ggctcatgca ccgacttgcg cgggatgatg cccggagcct
     4021 tcacgtccac gcggcggcgc tcggtgaact ggatcgggcc cttgccgtcg atcggctcgc
     4081 ccagcggatt gacgacgcgg cccagcaggc ccttgccgac cggaacgtcc acgatctcgc
     4141 ccagacggcg gacctcgtcg ccctcggcga tctggttgtc ct
//
LOCUS       DASVVN010000145         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_320185,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000145 DASVVN010000000
VERSION     DASVVN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320185"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VF686_02215"
     CDS             complement(<1..857)
                     /locus_tag="VF686_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8660653.1"
                     /translation="MFGKRTGQPGNVATAPRPAAPAPQPGPAAQPPAGIQTEAFAFGG
                     DPTVDQIQAAARKAESYAAKPASPAADRLDALAMRPKPASDVEAPQKGPGPKATPGYE
                     QLKKAQAVAEIVREQSDYYHATKTTIFNALMNTIDLAQLAQLDTKAASEEIRDIVAEL
                     VAIKNVSMSVAEQEHLVQDIVNDVLGYGPLEPLLARDDIADIMVNGASRVFIEVAGKV
                     QLTNVRFRDNAQLMNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGPT
                     LTIRKFKKD"
     gene            complement(861..2399)
                     /locus_tag="VF686_02220"
     CDS             complement(861..2399)
                     /locus_tag="VF686_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HEX8660654.1"
                     /translation="MSNAFAPQPFDEFDDEFDVDMGYVTPEAAAAAGPVVDEPAAFPG
                     EPSADQRPPLQFTPPAVAVQAEPPLRETDASVPAVAGFNPVGELVAGAASAFSAPTFS
                     GGQTAEVSVPRIAIHVFAERQDTLAAAERAGQDRRLSRATTQIRIGGVAAAVEAYQHE
                     PTPPLIVVECMKDPQTLLWEVDQLAEVCDAGTKVVVVGATNDILLFRELMRRGVSEYL
                     VAPLQPLQLIAAIGGLFADPAQPFVGRSIAFVGARGGTGASSVAHNTAYAMSERIGAN
                     TVIVDYDLPFGTAGLDFNQDPLSGVADALSQPDRLDPLLLDRMMVRCTDKLSLFAAPA
                     TLDVDWDISPEAFEEVTTQIRSTAPFVVLDLPHLWSGWMRRTLIAADEVVIVATPDLA
                     SLRNAKNMIDLIKQGRPNDAPPRLVLNQVGVPGRPEIPAKDFGAALGVHPSLIIPFDP
                     KTFGAAANNGQMILDAGAKSKAAEAFQTLAQIVSRRELPMLTGPKAKGESKSMFSGLF
                     KKKS"
BASE COUNT          373 a          817 c          961 g          427 t
ORIGIN      
        1 ttgtccttct tgaacttgcg gatcgtgagc gtcggcccgt cgatcgccag cggcggtgcg
       61 atgacgttga cgcgcgagcc gtccggcagg cgggcgtcgc agatcgggga gctctcgtcg
      121 acgcggcggc cgacctgcga cacgatccgc tggcagatgt tcatcagctg ggcgttgtcg
      181 cggaagcgga cgttggtcag ctggaccttg cctgcaactt cgatgaacac gcggctggcg
      241 ccattgacca tgatgtcggc gatgtcgtcg cgggccagca gcggctccag cggaccatag
      301 ccgaggacgt cgttgacgat gtcctggacc aggtgctcct gctcggccac cgacatggag
      361 acgttcttga tcgccaccag ctcggcgacg atgtcgcgga tctcttccga cgcggccttg
      421 gtgtcgagct gggccagctg ggccagatcg atcgtgttca tcagggcgtt gaagatcgtc
      481 gtcttggtgg cgtggtagta gtccgactgc tcgcggacga tctcggcgac ggcctgggcc
      541 ttcttcagct gttcatagcc cggcgtggcc ttggggcccg ggcccttctg gggcgcctcg
      601 acgtcgctcg ccggcttggg gcgcatggcc agggcgtcca gccggtcggc ggcgggggag
      661 gcgggcttgg cggcatagga ttccgccttg cgggccgcgg cctggatctg gtcgacggtc
      721 gggtcgccgc cgaaggcgaa ggcctcggtc tggatgcccg caggcggctg cgcggccggc
      781 ccgggctggg gcgcaggcgc ggccggacgc ggcgccgtcg cgacatttcc cggctgacct
      841 gtgcgcttgc cgaacacccg ttacgacttc ttcttgaaca ggccgctgaa catcgacttg
      901 ctctcgccct tggccttggg acccgtcagc atgggcagtt cgcggcgcga cacgatctgg
      961 gccagcgtct ggaaggcctc ggcggccttc gacttggcgc ccgcgtccag gatcatctgg
     1021 ccgttgttgg cggccgcgcc gaaggtcttg ggatcgaagg gaatgatcag gctggggtga
     1081 acgcccaggg cagcgccgaa atccttggcc ggtatttccg gtcggccggg cacgccgacc
     1141 tggttcagaa ccagccgcgg cggcgcgtcg ttgggacggc cctgtttgat caggtcgatc
     1201 atgttcttgg cgttgcgcag cgaggccagg tccggcgtcg cgacgatgac gacctcgtcg
     1261 gcggcgatca gggtgcggcg catccagccc gaccagaggt ggggcaggtc cagcacaacg
     1321 aaaggcgcgg tcgagcggat ctgggtcgtg acctcctcga acgcctcggg cgaaatgtcc
     1381 cagtcgacgt cgagcgtcgc gggagccgcg aacaggctga gcttgtcggt gcagcggacc
     1441 atcatccggt ccagcagcag cgggtcgagg cgatcaggct ggctgagcgc gtcggcgacg
     1501 ccgctgagcg ggtcctggtt gaaatcgaga ccagccgtgc cgaacggcag atcgtagtcg
     1561 acgatgacgg tgttggcgcc gatccgctcg gacatcgcat aggcggtgtt gtgcgccacc
     1621 gagctggcgc ccgtgccgcc ccgggcgccg acgaaggcga tcgagcggcc gacgaagggc
     1681 tgggccggat cggcgaacag gccgccgatg gcggcaatca actgcagcgg ctgcagcgga
     1741 gccaccagat attcgctgac gccgcggcgc atcagctcgc ggaacagaag gatgtcgttg
     1801 gtcgcgccga cgacgacgac cttggtgccg gcgtcgcaga cctcggccag ctgatcgact
     1861 tcccacagca gcgtctgcgg gtccttcatg cattcgacga cgatcagcgg cggtgtcggc
     1921 tcgtgctgat aggcctcgac ggcggcggcg acgccgccga tgcggatctg ggtcgtggcg
     1981 cgcgacaggc gacggtcctg tcctgcccgc tccgcggcgg ccagggtgtc ctggcgctcg
     2041 gcgaaaacgt ggatggcgat gcgcggcacc gacacttcgg cggtctggcc tccgctgaac
     2101 gtcggagccg agaaagccga tgcggcgccg gcgacaagtt cgcccacggg gttgaacccg
     2161 gccacggccg gaaccgaggc gtccgtttcg cgcaggggcg gctcggcctg gacggcgaca
     2221 gcaggcggcg tgaactgcag gggtgggcgc tggtcggcgg aaggctcgcc ggggaaggcg
     2281 gcaggttcat cgacaaccgg ccccgccgcc gcggcggcct ccggggtcac atagcccatg
     2341 tcgacgtcga actcgtcgtc gaattcgtcg aagggctggg gggcgaaggc gttgctcatg
     2401 aaattattcc accgcccgtg cgatctggcc gttcaccagg ggttcctgag ccgtcgagct
     2461 cggctggccc cggcggtaag cgtcgaacac cacggccgcc cgaccggaat cggccggcgt
     2521 catcggctgg gcgccgagga tgtcgcgcgg gttggcgatc tgcgcggcca tattggcg
//
LOCUS       DASVVN010000146         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_425988,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000146 DASVVN010000000
VERSION     DASVVN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425988"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF686_02225"
     CDS             complement(<1..515)
                     /locus_tag="VF686_02225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8660655.1"
                     /translation="MTTTVFLVRHGVHDQLQGRLCGRSAGVLLGDAGLAQADAAGRRL
                     AGAGVEVVQTSPLERTRQTAERIAHLCGAPLQIEEALMEIDFGDWNGRTFASLELEPL
                     WRAWNSQRDTARPPSGETMQEVQARLAAWLDRLVASGLRSVAAVSHADVIKAAVGLAT
                     GLSMRSHDRFE"
     gene            complement(512..1615)
                     /locus_tag="VF686_02230"
     CDS             complement(512..1615)
                     /locus_tag="VF686_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX8660656.1"
                     /translation="MGRKKVRIGVVGVGNCASSFVQGLSHYASAPGNQPPPGLMNVEL
                     GGYHVGDMEIASAFDVHAGKVGRDVAEAIFSGPNNTIRFAAPAPRGVQVMRGPTLDGI
                     GQYMAGDIEEADAPPVDVARALRESGTEVLVSYLPVGSQQATEFYAGEALKAGCAFVN
                     CIPVFIASDPAWAAQFRQRGLPIVGDDIKSQVGATIIHRVLANLMRERGVRIDRMYQL
                     NFGGNADFKNMLERERLTSKKISKTQAVTSQLDVPLPADDVHVGPSDFVPWLTDRKWA
                     HIRLEGTTFGDVPLNIELKLEVWDSPNSAGVVIDAVRCAKLAMDRGVSGPLIGPSSWF
                     MKSPPEQFTDEEARLRTLDFIEGLDQSAPRLAS"
BASE COUNT          291 a          607 c          584 g          291 t
ORIGIN      
        1 atctcgaacc ggtcgtggga ccgcatcgac agacccgtcg cgagaccgac cgcggccttg
       61 atgacgtcgg catggctgac ggcggccact gagcgcaggc cgctggcgac gagccgatcc
      121 agccaggcgg cgagccgcgc ctgcacctcc tgcatcgtct cgcccgacgg cggacgggcc
      181 gtgtcacgct ggctgttcca tgcgcgccac agcggctcca gctccaggct ggcgaaggtc
      241 cggccgttcc agtcgccgaa atcgatctcc atcagggcct cctcgatctg cagcggcgcg
      301 ccgcacaggt gggcgatacg ttccgcggtc tgccgggtcc gctccagtgg actggtctgc
      361 accacctcga cccccgcacc ggcgagacgt cggccagcgg catcggcctg ggccagtccg
      421 gcgtcgccga gcagaacgcc ggctgagcgg ccgcacagtc gtccctgcag ttgatcgtgg
      481 acgccgtgcc gcaccagaaa aacggtcgtc gtcacgaggc gaggcgcggc gcggactgat
      541 ccagaccctc gatgaagtcg agcgtccgca gccgggcctc ctcgtcggtg aactgttcgg
      601 gcggcgactt catgaaccag ctcgaagggc cgatcagcgg gccggatacg ccccgatcca
      661 tggccagctt ggcgcaacgc accgcgtcga tgaccactcc ggcgctgttg gggctgtccc
      721 agacctcgag cttcagttcg atgttcagcg gcacgtcgcc gaaggtggtg ccttccagcc
      781 ggatatgcgc ccatttccgg tcggtcagcc acggcacgaa atcgcttggc cccacgtgta
      841 cgtcgtccgc gggcagcggc acgtccagct ggctcgtcac ggcctgggtc ttggagatct
      901 tcttcgaggt cagccgctcg cgctcgagca tattcttaaa gtcggcgttg ccgccgaagt
      961 tgagctggta catccggtcg atgcggaccc cccgctcgcg catcagattg gcgagcacgc
     1021 ggtggatgat ggtggcgccg acctggctct tgatgtcgtc gccgacgatc ggcaggccgc
     1081 gctgccggaa ctgcgccgcc caggcgggat cggaggcgat aaacacgggg atgcagttga
     1141 cgaaggcgca accggccttc agcgcctcgc cggcgtagaa ctccgtcgcc tgctgcgatc
     1201 cgacgggcag gtaggacacc agcacctcgg tcccgctctc gcgcagggcc ctggcgacat
     1261 cgaccggcgg cgcgtcggct tcctcgatat cgccggccat atactggccg atgccatcga
     1321 gggtcggacc acgcatgacc tgcacgccgc ggggcgcggg agccgcgaac cgaatggtgt
     1381 tgttcgggcc gctgaagatc gcctcggcga catcgcggcc gaccttgccg gcgtgcacgt
     1441 cgaacgccga ggcgatctcc atgtcgccga cgtgatagcc gccgagctcg acgttcatca
     1501 gccccggcgg cggctggttg cccggcgccg acgcatagtg gctgagaccc tgcacgaatg
     1561 aagaggcgca gttgccgacg ccgacgacgc cgatgcgaac ctttttgcga cccatgggga
     1621 acctccagga cccgaccgcc ccatgcaagc cggacggttg gagaagcatc tacaaagctt
     1681 atggttccgc tgaccggcag cttgactaca gcaacgcagc gcttcggtta ttgtaacgct
     1741 actgcttggc tgtaattttg gcattcacta gca
//
LOCUS       DASVVN010000147         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_111247,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000147 DASVVN010000000
VERSION     DASVVN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111247"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..2487)
                     /locus_tag="VF686_02235"
     CDS             complement(217..2487)
                     /locus_tag="VF686_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8660657.1"
                     /translation="MRNKLLASTLFLAALSPFTAAMAQTSNGAPDPAILTPERVFANP
                     SLSGPVAKSVSLSPDGELVAFLRSREDDVDVLDLWAAPIGAGEPFKLINARALVPDAG
                     ELSEAEKARRERMRISARGVVEYSWDEQGRYILAPLEGDIFLASREGGAVRRLTETEA
                     DEIDAKVSPKGNYVSFVRDQDLVVVDLATSAETPITDDGEGLITWATAEFIAQEEMDR
                     DTGYWWSPDERYIALQRTDESPVDIVPRLDITGGGATVVEQRYPRAGRPNAVVELYVR
                     DIVGGGRVKVNLGADTDIYLARVNWSADGRTLYVQRESRDQKTLDLLSVDPATGASRV
                     ISTQRSNAWVSLNNDFKALEDGNFIWSSEETGWKHLYLYNREGRRLRAITRGDYPVKS
                     LNGVNQATGDVFFTASMRDGEELPIEQQLFRASLRRTVTPVAVTPAGGWWSASVNKTG
                     TAYVGNYTDLNTPAQSGLYRADGTFVRWIEENRLDADHPMYPYISRRGTVTFGTLPSH
                     GETLVWQMTTPPGFDPSKTYPVVMQVYGGPSGGGVKRGWQGATNQLLTEAGYIVFRLD
                     NRGEGDRSAAFKQALHLRMGQPEIEDQVLAANYLRSLPYVDDSRIAMMGWSYGGFMSL
                     MALTEPDMGLASAIAGAPPTEWSLYDTHYTERYMSTPQANAAGYAASDAIPRLPNLTG
                     RLLLMHGMADDNVILENSTRVIDALQASSTPFELMLYPGQRHGVRGNERQLQQWRTYL
                     DFLDRTIGTRAPAARD"
BASE COUNT          398 a          876 c          876 g          437 t
ORIGIN      
        1 cgtttcgggc aggaacgatc cttatgcacg gaacaggctg gccggaggtc cagccacggt
       61 cctcaagcat gtaggtcgcg cccctccggt ctgcctccgg cagagattcc agcggtcggc
      121 actggccctg atccatcagg acctgtgcgg ccgccggcgc cagcgccgcc gcccgcgcat
      181 gcgcgggcgt ggctgcgatc agcagagccc ccgcgatcag tcccgcgcgg cgggcgccct
      241 tgtaccgatc gtgcggtcca ggaaatcgag ataggtgcgc cattgctgta gctgcctttc
      301 attgccccgc acgccgtggc gctggccggg atagagcatc agttcaaacg gggtggacga
      361 ggcctgcagc gcgtcgatga cgcgggtgga gttctcgagg atgacgttgt cgtcggccat
      421 gccgtgcatc agcagcagcc gcccggtgag gttcggcaag cgcggaatgg cgtccgaggc
      481 cgcatagccg gcggcgttgg cctgcggcgt cgacatgtac cgttcggtat agtgggtgtc
      541 gtacaggctc cactcggtcg gcggcgcgcc ggcgatggcc gaggccagac ccatgtcggg
      601 ctcggtcagg gccatcaggc tcatgaagcc gccgtacgac cagcccatca tggcgatgcg
      661 gctgtcgtcg acgtagggca gcgaccgcag atagttcgcc gccagcacct gatcctcgat
      721 ctcgggctgg cccatccgca gatgcagcgc ctgtttgaac gccgccgacc gatccccctc
      781 gccgcggttg tccagccgga agacgatata gccggcctcc gtcagcagct ggttcgtcgc
      841 cccctgccag ccgcgtttga cgccgccgcc cgacgggccg ccatagacct gcatcaccac
      901 cggatatgtc ttcgacggat cgaagccggg gggcgtggtc atctgccaga ccagcgtctc
      961 gccatggctc ggcagggtcc cgaaggtcac agtcccgcgg cgcgagatgt agggatacat
     1021 cgggtgatcg gcgtccagcc ggttctcctc gatccagcgc acgaaggtcc cgtcggcgcg
     1081 gtacaggccc gactgcgccg gcgtgttcag gtcggtgtag ttgccgacat aggcggtgcc
     1141 ggtcttgttg accgaggcgc tccaccagcc gcctgcgggc gtcaccgcca ccggcgtcac
     1201 tgtccggcgt agcgaggcgc ggaacaactg ctgctcgatc ggcagttctt ccccgtcgcg
     1261 catcgaggcg gtgaagaaga cgtcgcccgt cgcctgattg acgccgttca gcgacttgac
     1321 cggatagtcc ccgcgcgtga tcgcgcgcag gcggcggcct tcgcggttgt agaggtacag
     1381 gtgcttccag cccgtctcct cggacgacca gatgaagttg ccgtcttcca gcgccttgaa
     1441 gtcgttgttc agcgacaccc aggcgttcga gcgctgggtc gagatcacgc gtgaagcccc
     1501 ggtcgcggga tcgacgctca gcagatccag cgtcttctgg tcccggctct cgcgttggac
     1561 gtacagcgtc cggccgtcgg ccgaccagtt cacgcgggcc agatagatgt cggtgtcagc
     1621 gcccagattg accttgaccc ggccgccgcc gacgatgtcg cgcacataca gttcgaccac
     1681 ggcgttgggc cggccggcgc gcggatagcg ctgctcgacc acggtcgcgc caccgcctgt
     1741 gatgtccagc cgcggcacga tatcgaccgg gctctcgtcc gtgcgctgca gggcgatgta
     1801 gcgctcgtcc gggctccacc agtagccagt gtcgcggtcc atctcttcct gggcgatgaa
     1861 ctcggcggtg gcccatgtga tcagaccttc gccgtcgtcg gtgatcgggg tctcggcgct
     1921 ggtcgccaga tcgacgacga ccagatcctg atcgcgcacg aaggagacgt agttgccctt
     1981 gggcgagacc ttggcgtcga tctcgtcggc ctcggtctcg gtcaggcggc gaacggcgcc
     2041 gccttcgcgg ctggccagaa agatgtcgcc ctccaacggg gcgagaatat agcggccctg
     2101 ctcgtcccac gaatattcga ccacgccccg cgcgctgatc cgcatccgct ctcgccgcgc
     2161 cttctcggcc tcggacagtt cgccagcgtc gggaaccaga gcccgcgcgt tgatcagctt
     2221 gaacggttcg ccggcgccga tgggcgcggc ccacagatcc agcacgtcca cgtcgtcctc
     2281 gcgcgagcgc aggaaggcca ccagctcccc gtcgggcgac aggctgaccg acttggccac
     2341 cggcccggac agggacggat tggcgaacac ccgctcgggc gtcaggatcg ccgggtccgg
     2401 cgccccgttc gaagtttgcg ccatggcggc ggtgaacggc gacaacgccg caaggaacag
     2461 ggtggaggcg aggagtttgt tgcgcatggg ccggacgcta gcggctgaaa actgcgacgt
     2521 catcccactt cttccttctc cccctgcggg agaaggtggc aggccgcagg cctgacggat
     2581 gaggggt
//
LOCUS       DASVVN010000148         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_215762,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000148 DASVVN010000000
VERSION     DASVVN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215762"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF686_02240"
     CDS             <1..339
                     /locus_tag="VF686_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660658.1"
                     /translation="QNWYAAGGLYSTVDDLLAFSQGLYGGRLVSAASLQGMLAPGLDE
                     YGFGQWVSTLEAGGTTHRFAQRPGRIMGANTLLLRMLDDDVTVVILANTNLVDTDRLG
                     FRIGRHVLAG"
     gene            complement(391..906)
                     /locus_tag="VF686_02245"
     CDS             complement(391..906)
                     /locus_tag="VF686_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660659.1"
                     /translation="MTQSDKASAAAETAQPIIDPATVVGWGVDADPENDPTWPMRDRS
                     ADDGPGLNWARPPLQEPEVEILQSVEHLRTPAVMGLSTPPAGLSGSVRRAAFRFSESQ
                     WGHWVLLMLADRINSVEGIAADVMRGRPPNPLVEMGMVKRSRSREAALAMAAVAGLAA
                     GGAIYLARRRR"
     gene            complement(919..>1782)
                     /locus_tag="VF686_02250"
     CDS             complement(919..>1782)
                     /locus_tag="VF686_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8660660.1"
                     /translation="CKQGLYGNCHESNPAATAVGGIFGYSHTAGGFDGGQAEYVRVPY
                     ADFGPTIIPDGMDLDDAVLLTDVVPTGYQAAEMGGIQTGDTVVVFGAGPVGLLAARCA
                     WLFGAGRVIVIDHHEYRLEFARSYCPAEVYDFREIGDPVVFLKKATGGLGADVCIDAV
                     GADASGNAWHTLFGKKLKLEAGNATPLHWAINSVKKGGVVSIVGVYGPTGSMVPIGNV
                     VNKGITIRANQASVKRLLPRLIDHVQAGRIDPKAIITHRLPLEDVAEAYHLFSSKLDG
                     CIKPVLIPNAA"
BASE COUNT          285 a          589 c          618 g          290 t
ORIGIN      
        1 cagaactggt acgcggcggg cgggctatat tcgacggtcg atgatctgct ggccttctcc
       61 caagggcttt acggcggtcg actcgtgtcc gcggccagcc tgcaggggat gcttgcgccg
      121 ggtctggacg aatacggctt cggtcagtgg gtttcgacgc tcgaagccgg cgggacgacc
      181 caccgcttcg cacagcggcc cgggcgcatc atgggcgcca acaccctgct gctgaggatg
      241 ctcgacgacg acgtgaccgt ggtcatcctg gccaacacca acctggtcga tacggacaga
      301 ctcggcttcc ggatcgggag gcacgttctg gccgggtgaa cggcgccggc gtgaaggatc
      361 cgctggaggg tcagacaccg ccgccagcat tcagcgtcga cggcgcgcca gatagatggc
      421 tccgccggcg gcgaggcccg ccactgcggc catcgccagc gcggcttcgc gggatcggga
      481 ccgtttgacc atgcccatct cgaccagcgg attcggcggg cggccccgca tcacatcggc
      541 ggcgataccc tccacgctgt tgatccggtc cgccagcatc agcaggaccc agtggcccca
      601 ctggctctcg ctgaaccgga aggcggcgcg ccggaccgag ccgctcagac cggccggcgg
      661 ggtcgacagg cccatgaccg ccggcgtccg cagatgttcg acggactgga gaatctcgac
      721 ctccggctcc tgaagcggcg gacgggccca gttcaggccc gggccatcgt ccgccgagcg
      781 gtccctcatc ggccaggtcg gatcattctc gggatcggcg tcgacgcccc agccgacgac
      841 tgtcgcgggg tcgatgatcg gttgggcggt ctcggcggcg gccgaggcct tgtcgctctg
      901 ggtcatctcg atctccagtc aggctgcgtt ggggatcagg acgggcttga tgcagccgtc
      961 cagcttggac gagaacaggt gataggcctc ggccacgtcc tccagcggga gacggtgggt
     1021 gatgatggcc ttggggtcga tccggcccgc ctgcacatgg tcgatcaggc gcggcagcag
     1081 ccgctttacc gaggcttggt tggcgcggat ggtgatgccc ttgttcacga cattgccgat
     1141 cggaaccatc gagccggtgg ggccgtagac gccgacgatc gacaccactc cgcccttctt
     1201 caccgaattg atggcccagt gcagcggcgt ggcgttgccg gcctcgagct tcagcttctt
     1261 gccgaacagg gtgtgccagg cgttgcccga ggcgtcggcg ccgacggcgt cgatacacac
     1321 atccgcgccc agtccgccgg tggccttctt gaggaagacg accggatcgc cgatctcgcg
     1381 gaagtcatag acctcggccg ggcaatagct tcgggcgaac tcgaggcggt attcgtggtg
     1441 atcgatgacg atgacccgcc ccgctccgaa cagccatgcg cagcgcgccg ccagcaggcc
     1501 gacggggccg gcgccgaaga cgacgacggt gtcaccggtc tggatgccgc ccatctcggc
     1561 ggcctgatag ccggtcggga cgacgtcggt cagcaggacg gcgtcgtcga ggtccatccc
     1621 gtcaggaatg atcgtcgggc cgaaatcggc atagggaaca cggacgtatt cggcctgacc
     1681 accgtcgaag ccgcccgccg tatgggagta gccgaagatc ccgccgacgg ccgtcgccgc
     1741 ggggttggac tcatggcagt tgccgtacag cccctgtttg ca
//
LOCUS       DASVVN010000149         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_452725,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000149 DASVVN010000000
VERSION     DASVVN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452725"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..948)
                     /locus_tag="VF686_02255"
     CDS             complement(127..948)
                     /locus_tag="VF686_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX8660661.1"
                     /translation="MAEGWSFPKGDLMRGKRGLVMGVANDKSIAWGIASQLAAQGAEL
                     CFTYMGEALERRVRPLAESIGVKFLAPADVTDDASMDAAFAEIERQWGTLDFVVHSVA
                     FANKNELQGSFVDNTTRDSFLLAMNISVFSFVDVARRASKLMPNGGSMVTMTYLGSER
                     VIPNYNTMGVAKAALEAATRYIARDLGPKGIRANAISAGAMRTLSLAGISGGRGMLSQ
                     GRAFSALKEDTSMEGVAGCALWLLSDLGRSATGETVHVDAGFHMIGLPDEAAASE"
     gene            complement(1021..>1576)
                     /locus_tag="VF686_02260"
     CDS             complement(1021..>1576)
                     /locus_tag="VF686_02260"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519208.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX8660662.1"
                     /translation="AGRDGFVIAGGAGVVVLEELERARARGAKIYAEVTGYAANSDGY
                     DMVAPSGEGASRCMRLALESAGGRKIDYLNPHGTSTPVGDSKEMQAVRDVFNGSPPLI
                     SSTKSLTGHSLGAAGAQEAIYCLLMMNNGFAAESAHIDQLDPEFTDLPILRERKDVEL
                     EHVMSNSFGFGGTNGCLILSRFGG"
BASE COUNT          223 a          574 c          534 g          245 t
ORIGIN      
        1 gccgcaaacg cagctcccga cgcattggaa acaccgggcc agctcagcag gtggcgtgtg
       61 tggccgcccc ctccaccgct ccgcggtccc cctcccccgc actcttcggt gcggggaagg
      121 aaccggctac tccgaagcgg ccgcctcgtc gggcaggccg atcatgtgga agccggcgtc
      181 gacgtggacg gtctcgccgg tggccgagcg gccgaggtcc gacagcagcc acagggcgca
      241 gccggccacc ccctccatcg aggtgtcttc cttgagcgcg ctgaaggcgc ggccctggga
      301 cagcatgccc cggccgccgg agatgccggc cagcgacagg gtgcgcatgg cccccgcgga
      361 gatggcgttg gcccggatgc ccttcggccc gaggtcgcgg gcgatgtagc gggtggccgc
      421 ctccagcgcc gccttggcca cgcccatggt gttgtagttc gggatcaccc gctcggagcc
      481 aaggtaggtc atggtcacca tcgacccgcc gttcggcatc agcttcgagg cccgccgcgc
      541 cacgtcgacg aagctgaaca ccgagatgtt catggccagc aggaagctgt cgcgcgtggt
      601 gttgtccacg aacgagccct gcagctcgtt cttgttggcg aaggccaccg agtggaccac
      661 gaagtccagc gtcccccact gccgctcgat ctcggcgaag gcggcgtcca tcgaggcgtc
      721 gtcggtgacg tcggccgggg ccaggaactt gaccccgatg ctttcggcca gcggccgcac
      781 ccgccgctcc agggcctcgc ccatgtaggt gaagcagagc tccgcccctt gcgccgccag
      841 ctgcgaggcg atgccccagg cgatggactt gtcgttggcc acgcccatga ccagcccgcg
      901 cttgccgcgc atcaggtcgc ccttgggaaa gctccagccc tcggccatcg tccgctccgt
      961 ctgtccgggc cgtctcttag ccgctcgccc gcggcaaggc gacaggggag cggcggccct
     1021 ttacccgccg aagcggctga ggatcaggca gccgttggtg ccgccgaagc cgaagctgtt
     1081 gctcatcaca tgctccagct cgacgtcctt gcgctcgcgc aggatgggca ggtcggtaaa
     1141 ttccgggtcc agctggtcga tgtgggcgct ctcggcggcg aagccgttgt tcatcatcag
     1201 caggcagtag atcgcctcct gcgccccggc cgcgcccagg ctgtgcccgg tcagcgactt
     1261 ggtggacgag atcagcggcg gtgagccgtt gaacacgtcg cggaccgcct gcatctcctt
     1321 gctgtcgccg accggcgtgc tggtgccgtg cgggttgagg tagtcgatct tgcgcccgcc
     1381 ggcgctctcc agcgccagcc gcatgcagcg cgaggccccc tcgcccgagg gcgcgaccat
     1441 gtcgtagccg tccgaattgg cggcgtagcc ggtcacctcg gcgtagatct tggcgccgcg
     1501 cgcacgggcg cgctccagtt cctcgagcac caccacgccc gcgccgcccg cgatgacgaa
     1561 cccgtcgcga ccggcg
//
LOCUS       DASVVN010000150         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_268183,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000150 DASVVN010000000
VERSION     DASVVN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268183"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..902)
                     /locus_tag="VF686_02265"
     CDS             complement(78..902)
                     /locus_tag="VF686_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8660663.1"
                     /translation="MTEITALLSDPAAWAALITLVIMEVVLGIDNLIFISILSNKLPP
                     EHRQRTRRIGIGLALIMRLGLLAAIGWIIGLTATVVDLGLAGDGWDTAFSWRDLILIA
                     GGLFLVWKATTEVHHAVDVSISDDLLEKNKTGAVINNVGAAIVQIIMLDLVFSIDSIL
                     TAVGMTDHVPIMMVAVIIAVAAMLFASDPLAEFIDKNPTVVMLALGFLLMIGMVLIAD
                     GFGVHVPKGYIYAAMAFSALVEGLNMMGRKASEKKQAALAARQAELNKAETAEPGV"
     gene            1054..>1993
                     /locus_tag="VF686_02270"
     CDS             1054..>1993
                     /locus_tag="VF686_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEX8660664.1"
                     /translation="MPSGLAALLDDVATIAKMAAASVDDVSAAAGRASMKAAGVVVDD
                     AAVTPGYAMGFSPQRELPIIGKIAIGSLKNKFLILLPGALLISAFAPWALTPLLMAGG
                     AYLCFEACEKLWEKLAGHGDGETPADELALSSEEQEKKMVGSAIRTDLILSAEITAIA
                     LADLADRPLATQAAALALVGLLITVAVYGAVALIVKMDDIGLHLAERRSAAKRALGRG
                     LVRAMPVVLSGLATIGTAAMLWVGGGILVHGLHEYHLDLVPGLIEGLAHAAGGVPLIG
                     PVTGWLATAAASAVVGLVVGGVIVAILHFIPRGKRAA"
BASE COUNT          307 a          657 c          698 g          331 t
ORIGIN      
        1 gagcggggcg gtcgtctgcg ccgccgccgg caggggcgtc agcagcgcga ggaagacaag
       61 gacggccgcc cgcatggtca cactcccggt tcggccgtct cggccttgtt cagttcggcc
      121 tgccgcgccg ccagcgccgc ctgtttcttc tcggaggcct tgcgacccat catgttcagc
      181 ccctcgacga gggccgagaa ggccatggcg gcgtagatat agcccttggg cacgtggacg
      241 ccgaatccgt cggcgatcag caccatgccg atcatcagca agaagccgag ggccagcatg
      301 accacggtcg ggttcttgtc gatgaactcg gccagcgggt ccgaggcgaa cagcatggcc
      361 gccacggcga tgatcacggc gaccatcatg atcggcacgt ggtcggtcat gccgacggcg
      421 gtcaggatgg agtcgatcga gaagaccaga tccagcatga tgatctggac gatggccgcg
      481 ccgacgttgt tgatcacggc gcccgtcttg ttcttctcca gaaggtcgtc gctgatcgag
      541 acgtcgaccg cgtggtggac ctcggtcgtc gccttccaca ccaggaacag accgccggcg
      601 atcaggatca ggtcgcgcca gctgaaggcg gtgtcccagc cgtcgccggc cagacccaga
      661 tcgaccacgg tggccgtcag gccgatgatc cagccgatcg ccgccagcag gcccagccgc
      721 atgatcaggg ccagtccgat gccgatccgg cgggtgcgct ggcggtgttc gggcggcagt
      781 ttgttcgaca ggatcgagat gaagatcaga ttgtcgatgc cgaggaccac ttccatgatc
      841 acgagcgtga tcagggcggc ccaggcggcg ggatcggaca ggagagctgt gatttcggtc
      901 atggacccgg gataggcgag gcggcgcggc cacgccagaa ttttcaaccg tagcagggcg
      961 cgtcagggcg ccgcgcccga ccggcgcctc tggacccggc ccgcgggctc gcctagccaa
     1021 gagcgaactg catttgtttt tgtcaggtga cgcgtgccct caggtcttgc cgcccttctc
     1081 gacgacgtcg ccaccatcgc caagatggcc gcggcctcgg tcgacgacgt gtccgccgcc
     1141 gccggccgcg ccagcatgaa ggccgccggc gtggtggtcg acgacgccgc tgtgacgccg
     1201 ggctacgcca tgggcttttc gccccagcgc gagctgccca tcatcggcaa gatcgccatc
     1261 gggtcgttga agaacaaatt cctgatcctc ctgcccggtg cgctgctgat cagcgccttt
     1321 gcgccctggg cgctgacgcc gctcctgatg gccggcggcg cctatctctg cttcgaggcg
     1381 tgcgagaagc tgtgggagaa gttggcgggc cacggcgacg gcgagacccc ggcggacgag
     1441 ctggccctgt cgtccgagga acaggaaaag aagatggtgg gttcggccat ccgcaccgac
     1501 ctgatccttt cggccgagat caccgccatc gccttggcgg acctggccga ccgtccgctg
     1561 gcgacccagg cggcggccct ggcccttgtc ggcctgctga tcaccgtggc ggtctatggc
     1621 gccgtggccc tgatcgtgaa gatggacgac atcggcctgc atctggccga gcgccgctcg
     1681 gctgcgaagc gcgcgctggg ccggggactg gtccgcgcca tgccggtggt gctgagcggg
     1741 ctggcgacca tcggcaccgc agccatgctg tgggtcggcg gagggattct ggtccacggg
     1801 ctgcacgaat accacctcga tctggtgccg ggcctgatcg agggtctggc tcacgccgcc
     1861 ggcggtgtgc cgctgatcgg tcccgtgacc ggatggctgg cgacggcggc cgcctcggcg
     1921 gtggtcggtc tcgtcgtggg cggagtcatc gtggcgattc tgcatttcat cccgcgcggc
     1981 aagcgcgccg ccc
//
LOCUS       DASVVN010000151         4134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_188884,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000151 DASVVN010000000
VERSION     DASVVN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188884"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1902
                     /locus_tag="VF686_02275"
     CDS             136..1902
                     /locus_tag="VF686_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX8660665.1"
                     /translation="MIHLPHRSVRALVAVLVAAGLALGGCGGIGQGSAGPAGVAPTGP
                     VPAAPVARGPFVAAANPLAVEAGLKVLREGGSAVDAAVAIQAVLGLVEPQSSGLGGGA
                     FMMMFDAATGEVTSYDGRETAPAAATPELFYENGRPLGVGDAVLSGRSTGAPGAVAML
                     AMAHKVHGRLPWNALFGDAERLARDGFVVSPRLAGFIAATRGQARTRWANAYFTKPDG
                     NRYVAGDVLKNPAYADTVAELAAGGAEGFYNGRVAREIAATVAQEPRPGALTVEDIAA
                     YEPVERGALCRPYRVYVVCVPPPPSSGVAVLQLLAMAERTPDIHGGPDSAEAWTAFAQ
                     LQRLMYADRDRYVGDPGFVRVPVQGLLDPDYVAARAALAPGLTGAAQAGTPPGGVFAG
                     PDATREPAGTSHLVVVDAQGNAVSMTTTVESVFGSGRMAAGFFLNNQLTDFSFSPTRP
                     DGAPAANAVAAGKRPRSSMAPVLVFDRQGRLLGAFGSPGGTSILAYNAKALIAVLDWG
                     LPVQAAVDLPNLVARGDGFGADTDRFSEALRTGLAARGVVLTPNASENSGLHGAIWRQ
                     GTTGWGWDGGADDRREGVARTE"
     gene            complement(1953..2786)
                     /locus_tag="VF686_02280"
     CDS             complement(1953..2786)
                     /locus_tag="VF686_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HEX8660666.1"
                     /translation="MATGALWAPAFAAASQDAQADPWKVAPERPTNVADLLTRLDRDA
                     RYASDPAWDTAGGWTGYAPERDRTSQWAASSRDVWFESGGYEDRLRLRTEGSVRRLDG
                     SPLPPGPLDPATFDAEHYDLTYTRGWTETLGQTASGLDVTLTPHVGVGVGTRGGTTEA
                     GATLRIGRGIEKLVPDGGDAFGERPRWYLYAAGSGRAVGYNFARNRDGDFARSGYSQD
                     SGNFLGDASVGVAMRRGPVHTSVSVAYREIETEGLRAGHGVDTDVSEGVIAFQLSIKP
                     E"
     gene            complement(2998..>4134)
                     /locus_tag="VF686_02285"
     CDS             complement(2998..>4134)
                     /locus_tag="VF686_02285"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX8660667.1"
                     /translation="KTVDARALWQKILEIRLQTGEPYLIFSDTVNRQMPQHQRDLGLS
                     VKQSNLCSEIMLHTGPDHLGIDRTAVCCLSSVNAEMFLEWREEPRFIEDVMRFLDNVL
                     EDFIRRAPPAMAAAVYSAKRERSVGLGLMGFHSFLQQQGVAFESAMAKSWNMRLFKHL
                     RREADKASRVLAEEKGACEDAAERGVMERFSHKLAIAPTASISIICGGTSAGIEPIPA
                     NIYTHKTLSGSFAVKNPYLERILGEKGIDTSEVWNSILEHEGSVQHLEGLSDDEKAIF
                     KTAFELDQRWIVELSADRAPEICQAQSINLFIPGDVNKWDLHMLHWSAWEKGVKSLYY
                     LRSKSVQRAAFAGAEDKAEAEVDPNQPDLFSAARTDYDECLACQ"
BASE COUNT          580 a         1432 c         1481 g          641 t
ORIGIN      
        1 agaagacggg ttttccgcct tgttgcatcg ttgggcgcca gaagggcgcg acgcggggct
       61 ttcgttcgcg cgggcggcgg ggcagggtgc ccctcctctc ccgagttcgt tcgccgaaac
      121 agacctgagc cctccatgat ccaccttccg caccggtccg tccgcgccct tgtggctgtg
      181 ctcgtcgcgg cgggtctggc gctgggcggt tgcggcggca ttggccaggg atcggccggt
      241 cccgccggtg tcgcgccgac cggcccggtc cccgccgcgc ccgtcgcccg cggtccgttc
      301 gtggcggccg ccaatccgct ggcggtcgag gcgggactga aggtgttgcg cgagggcggg
      361 tccgccgtgg acgccgccgt ggccatccag gcggtgctgg gcctcgtcga gccgcagtcg
      421 tccggactgg gcggcggggc cttcatgatg atgttcgacg ccgccaccgg cgaggtcacc
      481 agctatgacg gccgcgaaac cgcgccggcc gccgcgacgc ccgaactgtt ctacgagaac
      541 ggacgtccgc tgggcgtcgg agacgcggtc ctgagcgggc gctcgaccgg cgcgcccggc
      601 gccgtggcca tgctggcgat ggcgcacaag gtccacggac ggctgccgtg gaacgccctg
      661 ttcggcgacg ccgaacggct ggcccgcgac ggcttcgtgg tcagcccgcg gctggccggc
      721 ttcatcgccg ccacgcgggg gcaggcccgc acccgctggg ccaacgccta tttcaccaaa
      781 ccggacggga accgctacgt cgccggcgac gtgctgaaaa acccggccta cgccgacacc
      841 gtggccgagc tcgccgcggg cggggccgag ggcttctaca acggccgggt cgcgcgcgag
      901 atcgccgcca cggtggcgca ggagccccgg cccggcgccc tgacggtcga agacatcgcg
      961 gcctacgaac cggtcgagcg cggggcactg tgccgtccct accgggtcta tgtcgtctgc
     1021 gtgccgccgc cgccgtccag cggcgtggcg gtgctccaac tgctggccat ggccgagcgg
     1081 acgccggaca ttcacggcgg tcccgacagc gccgaggcct ggaccgcctt cgcccagctg
     1141 cagcggctga tgtacgccga ccgcgaccgc tatgtcggcg atccgggctt cgtccgggtg
     1201 ccggtgcagg gcctgctgga tcctgactat gtcgcggccc gcgccgccct ggcccccggc
     1261 ctgaccggcg cggcccaggc cggcacgccg cccggcgggg tgttcgccgg gcccgacgcc
     1321 acgcgcgagc cggccggaac ctcgcatctc gtcgtcgtcg atgcgcaagg caatgcggtc
     1381 agcatgacca ccacggtcga aagcgtgttc ggctcgggcc ggatggcggc gggcttcttc
     1441 ctcaacaacc agctgaccga cttctccttc agccccacgc ggcctgacgg cgcgccggcg
     1501 gccaatgcgg tggcggcggg caagcggccg cggtcgtcga tggcgccggt cctcgtcttc
     1561 gaccgtcagg gccggctgct gggggcgttc ggctcgcccg gcgggacctc catcctggcc
     1621 tataacgcca aggccctgat cgccgtgctg gactggggcc tgccggtcca ggccgcggtc
     1681 gacctgccca atctggtggc ccggggcgat ggcttcggcg ccgacacgga ccgcttttcc
     1741 gaagcgctgc gcaccggtct ggcggcgcgc ggcgtcgtgc tcacccccaa cgccagcgag
     1801 aactcgggcc tgcatggcgc gatctggcgt cagggaacga cgggctgggg ctgggacggc
     1861 ggcgcggacg accggcgcga aggcgtggcg cggacggagt agcgcgctca agcaggccgc
     1921 gaccgcgtcg cggccgtagc gcacaagaat ggttattccg gcttgatgct cagctggaag
     1981 gcgatcacgc cctcggacac gtcggtgtcg acgccgtggc ccgcacgcag gccctcggtc
     2041 tcgatttcgc gataggcgac gctgaccgag gtgtgcaccg gcccccggcg catggccacg
     2101 cccaccgagg cgtcgccgag gaaattgccc gagtcctggc tgtagcccga ccgtgcgaag
     2161 tcgccgtcgc ggttgcgggc gaaattatag ccgacggccc ggcccgagcc cgccgcgtac
     2221 agataccagc gcgggcgctc gccgaaggcg tcgccgccgt ccggaaccag tttctcgatg
     2281 ccgcggccga tgcgcagggt ggcgccggcc tcggtcgtgc cgccgcgggt tccgaccccg
     2341 accccgacat gcggcgtcag ggtgacgtcc agcccggacg cggtctggcc aagggtctcg
     2401 gtccagcccc gcgtataggt caggtcatag tgttcggcgt cgaaggtcgc ggggtcgagc
     2461 ggtccgggcg gcaggggcga gccgtcgaga cggcggacgg agccttcggt gcgcaggcgc
     2521 agacggtctt catagccgcc gctttcaaac cagacgtccc ggctcgaggc ggcccattgc
     2581 gaggtccggt cgcgctcggg cgcatagccg gtccagccgc ctgcggtgtc ccatgcaggg
     2641 tcgctggcgt agcgggcgtc gcgatcgagg cgggtcagaa ggtcggcgac gttggtcggg
     2701 cgttcggggg cgaccttcca gggatcggcc tgggcgtcct gggaagcggc ggcgaacgcc
     2761 ggggcccaca gggctccagt cgccaacact ccggcaagga tcgccgtcat acgcatcaac
     2821 ggttcctccc cgatccgtta acgactccga ccctgcacga tgcaggccgt ctcgccaaca
     2881 ccctttcgag cgtcacggct cggccgatca acgtgaaccg ggggagggag ttccgcaatc
     2941 gggtgaaccc ctcaggcgag tttcagcctc gcctgactgg ccgccttgcc acccgcctta
     3001 ctggcaggcc aggcactcgt catagtcggt gcgcgccgcc gagaagagat cgggctggtt
     3061 cggatcgacc tcggcctcgg ccttgtcttc cgcgccggcg aaggcggccc gctgaaccga
     3121 cttggagcgc aggtagtaga gcgacttgac gcccttctcc cacgccgacc agtgcagcat
     3181 gtgcaggtcc catttgttga cgtcgccggg gatgaacagg ttgatcgact gggcctggca
     3241 gatttccggc gcccggtcgg cggacagctc gacgatccag cgctggtcca gttcgaaggc
     3301 ggtcttgaag atagccttct cgtcgtcgct caggccttcg agatgctgca ccgagccctc
     3361 gtgctcgagg atcgagttcc acacttccga ggtgtcgatg cccttttcgc cgaggatccg
     3421 ctccagatag gggttcttga ccgcgaacga gcccgacagg gtcttgtgcg tatagatgtt
     3481 ggccgggatg ggctcgatgc cggcgctggt gccgccgcag atgatcgaga tcgaggcggt
     3541 cggggcgatg gccagcttgt ggctgaaccg ctccatgacg ccgcgctcgg ccgcgtcctc
     3601 gcaggcgccc ttctcctcgg ccaggacacg gctggccttg tcggcctcgc ggcgcagatg
     3661 cttgaacagc cgcatgttcc acgacttggc catggcggac tcgaaggcca cgccctgctg
     3721 ctgcaggaag gaatggaagc ccatcaggcc cagacccacc gaacgctcgc gcttggcgga
     3781 atagacggcg gcggccatcg ccggcggggc gcggcggatg aagtcttcca gcacattgtc
     3841 gaggaaacgc atcacgtcct cgatgaagcg gggctcctcg cgccactcca ggaacatctc
     3901 ggcgttgacc gacgacaggc aacagacggc ggtccggtcg atgcccagat ggtcagggcc
     3961 ggtgtgcagc atgatctcgc tgcacaggtt cgactgtttc accgacaggc ccagatcgcg
     4021 ctgatgctgc ggcatctggc ggttcacggt gtcggagaag atcagatagg gctcgccggt
     4081 ctgcaggcgg atctcgagga tcttctgcca cagggcgcgg gcgtcgacgg tctt
//
LOCUS       DASVVN010000152         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_320483,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000152 DASVVN010000000
VERSION     DASVVN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320483"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..512
                     /gene="ppa"
                     /locus_tag="VF686_02290"
     CDS             3..512
                     /gene="ppa"
                     /locus_tag="VF686_02290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX8660668.1"
                     /translation="MEIPQGGEPVKYEMDKASGALFVDRFLHTAMYYPGNYGFIPHTL
                     SDDGDPCDVIVLNPTPVVPGCIIRSRPIGVLKMEDEAGGDEKILAVPVDKLNPYYTDI
                     ASYRQLPAILVEQIEHFFTRYKDLEKNKSVTVKGWGDAGEAAELIAAGMRAHQDKLKA
                     AAGKAANAA"
     gene            550..2421
                     /gene="cobT"
                     /locus_tag="VF686_02295"
     CDS             550..2421
                     /gene="cobT"
                     /locus_tag="VF686_02295"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX8660669.1"
                     /translation="MAAAETPADIFKRALAHAARALAEQSELEVAFGSDGPKLANGVL
                     TLPHPARDPGPNGNAALRGQADRLALRLANHDESLHARLRPVDTRAAEVFEAVEQARV
                     EAVGSTSLAGVRANMNAALLTRLEKMGALRAEAERVPVAEAVALLVRERLTGQRAPDG
                     AGTMLDLVRTALEDKAGLKLNALAEVAGDQTIFAERLKDVLRALDLDPGDGRGDDASD
                     DQGDEEPDPQDPDAADDQDEEDDAGGGEGESMDGSTDDKDQGEDRDSRPDGAPGDPDG
                     EASDDGPDVNEGSLPNRQQTMDDGRAIDAYRVFTIAFDEVVEAADLCDPNELERLRAL
                     LDQQLTALSSVVSRLANKLQRRLMAQQNRSWHFDLEEGVLDTARLTRIITDPTSPLSF
                     KAESESPFRDTVVTLLLDNSGSMRGRPIMVAAVCADILARTLERCGVKVEILGFTTRA
                     WKGGQAREAWITAGKPANPGRLNDLRHIIYKSADAPWRRAKKSLGLMMREGLLKENID
                     GEALTWAHERLLARTESRRILMVISDGSPVDDSTQSANAALYLDKHLRQVIAEIETRS
                     PVELMAIGIGHDVTRWYRRAVTIVDVEQLAGAMVENLAALFDAEPKAVASGRRRKAA"
     gene            2418..3389
                     /locus_tag="VF686_02300"
     CDS             2418..3389
                     /locus_tag="VF686_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8660670.1"
                     /translation="MRRLAAAALSLLLSACAGAGVVSAPWRGAADGWTPVAAEIRAVG
                     LGIPGGVRLAPGVRFAGGVQIIAPRGDRLHGLSDLKLVDGDFLAVTDGGDLVRGRLGF
                     DRRGRLAGLSDLRLRGLTAGDGRAFPTKSDGDAESLAITAGGELVIGFEQTPRLWSYG
                     PLHAPRDRPSLLAVPPIAPGNDGLEALAAGPNGLRAAAEAGGVWDCTPARCTEVMPPP
                     ANPPAVSDWRVTGMDRDPAGDGWFVIERLYREPFDMRARLRRMDTSGALGPVLASLSL
                     PSATDNFEGVAAVTGASGTRLYILSDDNDNPRQRTLLLAFDLEIRRP"
     gene            complement(3394..3597)
                     /locus_tag="VF686_02305"
     CDS             complement(3394..3597)
                     /locus_tag="VF686_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660671.1"
                     /translation="MAEQNNLPTVTVEGHRAAPTQTCRTEAVTGSRFGRRVCRNNVQS
                     DDDRINSREMLRRLQGARMPDGG"
     gene            complement(3773..4381)
                     /locus_tag="VF686_02310"
     CDS             complement(3773..4381)
                     /locus_tag="VF686_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX8660672.1"
                     /translation="MPERQEPEHPADDPLTDRRIPLARPGRGASKRRRAEILRVDHAG
                     EYAAVHIYRAQKAVFDGRAGKTALAADMGEMQDQEAVHLARFDALLNAERVRPTLMTP
                     VWRLAAMALGTGTALMGEKAAHACTEAVESVIEEHYADQIAELRDRDPALAAELSVFR
                     DEELAHHDHAVDQGARDAPGYDLLSAVIKTSCRVAIKISEKV"
     gene            complement(4359..4892)
                     /locus_tag="VF686_02315"
     CDS             complement(4359..4892)
                     /locus_tag="VF686_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX8660673.1"
                     /translation="MRGLYRLLTRWWTAFALAASLAMLGAAHAFERFAGLAPCNLCLK
                     QREVYWGAVAIALTATVWHLVSRGSRGTPRIAAFLLAVVFATGTITAVFHMGGELKWW
                     ELPATCAGGGEVNLEGLAALALGTGPVENLVFCDTVTWRFLGLSMAGWNALISAALAV
                     FSLLAAKRPKDARAPRT"
     gene            complement(4944..>5437)
                     /locus_tag="VF686_02320"
     CDS             complement(4944..>5437)
                     /locus_tag="VF686_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX8660674.1"
                     /translation="ESSFFPIPPDVMLAPMILARPDRAYFYAGVCTLASVLGGILGYA
                     IGYFLTDVGMTILAWLGKADTFEASKSLFQQHGAWVILIKGLTPIPFKLITIASGIFQ
                     FNFALFVALCVVTRGARFFAEAFILKRWGPPMLEMVEKRLAMWTVIGLVVLIGAIAAV
                     KLL"
BASE COUNT          876 a         1818 c         1942 g          801 t
ORIGIN      
        1 tcatcgagat cccgcagggc ggcgagccgg tgaagtacga gatggacaag gcctctggcg
       61 ccctgttcgt cgaccgtttc ctgcacacgg cgatgtacta tccgggcaac tacggtttca
      121 tcccgcacac cctgtcggat gacggcgatc cctgcgacgt gatcgtgctg aacccgacgc
      181 cggtggttcc gggctgcatc atccgctcgc gcccgatcgg ggtgctgaag atggaagacg
      241 aggccggcgg cgacgagaag atcctcgccg tgccggtcga caagctgaac ccctactaca
      301 ccgacatcgc cagctatcgg cagctaccgg ccattctggt cgagcagatc gagcatttct
      361 tcacccgcta caaggatctg gagaagaaca agtcggtgac cgtgaagggc tggggcgacg
      421 ccggcgaggc tgccgaactg atcgccgccg gcatgcgcgc ccatcaggac aagctgaaag
      481 ccgccgctgg caaggccgcc aacgcggcgt gaggtttgcg caagggggcg gttggtctat
      541 ctgaccgtca tggccgccgc cgaaaccccc gcagacatct tcaagcgcgc cctcgcccat
      601 gcggcgcggg cgctggcgga gcagtccgag cttgaggtcg ccttcggctc ggacgggccg
      661 aaactggcca atggcgtcct gacgctgccc catcccgcgc gcgatccggg cccgaacggc
      721 aatgcggccc tgcgcggtca ggccgaccgg ctggccttgc gtctggccaa tcacgacgaa
      781 agcctgcatg cccgtctgcg gcccgtcgac acgcgggctg ctgaagtgtt cgaggcggtg
      841 gagcaggccc gggtcgaggc cgtgggctcg acctccctgg cgggcgttcg ggccaatatg
      901 aacgcggccc tgctgacccg gctggagaag atgggagcct tgcgcgcgga agctgagcgc
      961 gtgcccgtgg ccgaggccgt ggccctgctg gttcgcgaac ggctcaccgg gcagagggcg
     1021 ccggacggcg ccggaaccat gctggacctc gtgcgcacgg cgctggagga caaggcgggg
     1081 ctcaagctga acgccctggc cgaggtcgcg ggcgaccaga ccatcttcgc tgaacggctg
     1141 aaggacgtgc tgcgcgcgct ggatctggat cccggcgacg ggcgcggcga cgacgcctcg
     1201 gacgatcagg gcgacgagga gcccgatccg caggatccgg acgccgccga cgatcaggac
     1261 gaggaagacg acgccggcgg cggcgagggc gagtcgatgg acggctcgac cgacgacaag
     1321 gatcagggcg aggaccgcga cagccgtccg gacggcgcac ccggcgatcc cgacggcgag
     1381 gcctcggacg acggccccga cgtcaacgag ggctctctcc ccaaccgcca gcagaccatg
     1441 gacgacggtc gggccatcga tgcctatcgc gtcttcacca tcgccttcga cgaggtcgtg
     1501 gaggccgccg acctgtgcga cccgaacgag ctcgagcgtc tgcgcgccct gctggaccag
     1561 cagctcacgg ccctgtcgtc ggtagtgtcg cgtctggcca acaagctgca gcgccgcctg
     1621 atggcgcagc agaaccgcag ctggcatttc gatctcgagg agggggtgct ggacaccgcc
     1681 cgtctgaccc ggatcatcac cgacccgacc agcccgctga gcttcaaggc cgagagcgaa
     1741 agcccgttcc gcgacacggt ggtgaccctg cttctggaca actccggctc gatgcgcggg
     1801 cggccgatca tggtcgcggc ggtctgcgcc gacatcctgg cgcggacgct ggaacgctgc
     1861 ggcgtcaagg tcgagattct cggcttcacc acccgggcgt ggaagggcgg tcaggcgcgc
     1921 gaggcctgga tcaccgccgg caagcccgcc aatccgggcc gtctgaacga cctgcgccac
     1981 atcatctaca agtctgccga cgcgccttgg cgccgggcca agaagagcct cggcctgatg
     2041 atgcgcgagg gcctgctgaa ggaaaacatc gacggcgagg ccctgacctg ggcgcatgag
     2101 cggctgctgg cccgcacgga gtcgcgacgc atcctgatgg tcatctcgga cggctcgccc
     2161 gtcgacgatt ccacccagtc ggccaatgcg gccctgtatc tggacaagca cctgcgtcag
     2221 gtcatcgccg agatcgagac ccggtcgccg gtcgaactga tggccatcgg catcggccat
     2281 gacgtgaccc gctggtaccg ccgcgccgtc accatcgtgg atgtcgaaca gctggccggg
     2341 gccatggtcg agaacctggc cgccctgttc gacgccgagc ccaaggccgt ggcctcgggg
     2401 cgccgtcgga aggccgcgtg aggcggctcg ccgccgccgc actgtccctg ctgttgagcg
     2461 cctgcgcggg cgcgggcgtc gtctccgcgc cctggcgggg cgcggcggac ggctggacgc
     2521 cggtcgcggc cgaaatccgc gctgtcggtc tgggcattcc gggcggtgtc agactggctc
     2581 cgggcgtgcg tttcgcaggc ggcgtccaga tcatcgcgcc gcgcggcgac cggctgcacg
     2641 gcctgtcgga tctgaaactg gtggatggag acttcctcgc ggtcaccgac gggggcgatc
     2701 tggtaagggg ccgactgggg ttcgaccggc gcggacgact cgcgggcctg tcggatctgc
     2761 ggctgcgcgg gctgacggcc ggggacgggc gcgccttccc gaccaagtcc gacggcgacg
     2821 ccgaaagtct ggcgatcacg gcgggcggcg agctggtcat cggcttcgaa cagacgccgc
     2881 gcctgtggtc gtacgggccg ctccacgccc cgagggacag gccgtcgctg ctggcggttc
     2941 ccccgatcgc gccgggtaac gacggtctgg aagcgctggc ggcggggccc aacggcctgc
     3001 gggccgcggc ggaagccggc ggcgtgtggg attgcacgcc tgcgcgctgc acagaggtca
     3061 tgccgccccc cgcgaaccct ccggcggttt ccgactggcg cgtcaccggc atggaccgcg
     3121 acccggcagg cgatggctgg ttcgtgatcg agcgcctgta ccgcgagccg ttcgacatgc
     3181 gcgcccgcct gcgccgcatg gacacgtcag gcgccttggg ccccgtgctc gcgagcctca
     3241 gcctgccgtc ggcgacggac aatttcgagg gcgtggcggc ggtgacgggc gcgtccggga
     3301 cacgcctcta catcctgtcg gacgacaacg acaatccgcg tcagcggacc ctgctgttgg
     3361 cctttgatct ggagatccgg agaccttgag gggtcagccg ccgtccggca tgcgcgcgcc
     3421 ttgcaggcgg cgcagcattt cgcggctgtt gatccggtcg tcatcggact ggacgttatt
     3481 gcggcagacc cggcggccga aacgtgaccc cgtgaccgcc tcggtgcggc aggtctgggt
     3541 cggcgccgct cggtggccct ctacagtgac ggtggggagg ttgttctgtt cagccacagt
     3601 cacggattgc ggcgtggccg caggcgggac gggcgcgcct gtcgtgggag gcgtttgctg
     3661 cagaacaaga gcggaggcga acaagacggc ggggaacaac accagggagc tcctgtgaaa
     3721 acgttgatcg cataatgcca gcagcgccgc ggatcgcaac ccgggcgtgc ggtcagacct
     3781 tttcgctgat ctttatggca acacggcagc tggtctttat gaccgcgctg agcagatcat
     3841 aaccgggcgc gtcgcgggcg ccctggtcga cggcgtggtc gtggtgggcc agttcctcgt
     3901 cgcggaacac cgacaattcc gccgccagcg ccggatcgcg gtcgcgcagc tcggcgatct
     3961 gatcggcgta gtgttcctcg atcacgctct cgaccgcctc ggtgcaggcg tgggcggcct
     4021 tctcgcccat cagggcggtg cctgtcccaa gcgccatggc cgccagccgc cagaccggcg
     4081 tcatcagggt ggggcgcacc cgttcggcgt tcagcagggc gtcgaagcgg gccagatgca
     4141 ccgcttcctg gtcctgcatc tcacccatgt cggcggccag ggccgtcttg ccggcgcggc
     4201 cgtcgaacac ggccttctgg gcgcggtaga tatgaacggc ggcatattcg cccgcatggt
     4261 cgacgcgcag gatctcggcc cggcgacgct tgctggcgcc gcgccccggc cgggccagcg
     4321 gaatgcgccg gtccgtcagg ggatcatctg cggggtgctc aggttcttgg cgctctggca
     4381 tccttgggcc tcttggcggc gagaaggctg aacacggcga gggccgccga gatcagggcg
     4441 ttccatcctg ccatcgacag gccgaggaag cgccacgtca ccgtgtcgca gaagaccagg
     4501 ttctcgacag gtccggtgcc cagcgccagc gcggccagtc cctcgagatt gacctcgccg
     4561 ccgccggcgc aggtcgccgg cagctcccac cacttcagct cgccgcccat gtggaagacg
     4621 gcggtgatgg ttccggtggc gaagacgacg gccagcagga aggcggcgat acggggcgtg
     4681 ccccggctgc cgcggctgac cagatgccag acggtggcgg tcagggcgat ggccacggcg
     4741 ccccagtaga cctcgcgctg cttgaggcac aggttgcagg gggcgagccc ggcgaatcgc
     4801 tcgaacgcat gcgctgcgcc cagcatcgcc agcgaggcgg cgagggcgaa ggcggtccac
     4861 caccgcgtca ggagtcgata aaggcctctc atcctacgat gttctagccg ggccgggccg
     4921 ccgcgtcgat gcggctcgcc ggatcagaga agcttcaccg ccgctatggc gccgatcagc
     4981 accaccaagc cgatcacggt ccacatggcc agccgcttct cgaccatctc aagcatcggc
     5041 ggaccccagc gtttgaggat gaaggcctcg gcgaagaaac gggccccgcg ggttacgacg
     5101 cacagggcga cgaacagggc gaagttgaac tggaagatgc ctgaggcgat ggtgatcagc
     5161 ttgaacggga tcggtgtcag gcccttgatc aggatcaccc aggcgccgtg ctgctggaac
     5221 agggacttgg acgcctcgaa ggtgtccgcc ttgcccagcc aggccaggat cgtcatgccg
     5281 acgtcggtca ggaagtagcc gatggcatag cccagtatcc cgcccaagac ggaggccagg
     5341 gtgcagacgc cggcgtagaa ataggcccgg tccggccgcg ccaggatcat cggcgccagc
     5401 atgacgtcgg gcggaatggg gaagaaggag ctctcgg
//
LOCUS       DASVVN010000153         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_163780,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000153 DASVVN010000000
VERSION     DASVVN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163780"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VF686_02325"
     CDS             complement(<1..1163)
                     /locus_tag="VF686_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX8660675.1"
                     /translation="MSADATPAPVPRRPRRTGGRRILIVAGAVLCVLLILFAVVWLNR
                     RAATRQILIGWLEQRGVDADMDIERLEMDGLVARIRIGDPANPDVLIERVEVDYGIGA
                     PWSKGGLGVTPTRIRLVRPVVRASVRGGKLTFGSLDPLIEEFTGRPPRPDSRSPLVIV
                     ERARVRLDTDYGPANILGDARVDNGKLMRLVARMPATAFKSGEAEARGLSAAVDLTTT
                     GDRIAVRFDATAEHATMEGLAGDTARLVVTGNLPYPDLKGRRGDGRAAINAAFTAARL
                     SSGGTTARGTDARLGFTGQTTGWIETFRIAGDGDLDVRAARVDGPGTAASGIRLRLDH
                     ADTAFSRDAKGFGWRLQGPATVMAARATGAGLEGTGVTLRSAGFTLGGRNAAL"
     gene            1586..2929
                     /locus_tag="VF686_02330"
     CDS             1586..2929
                     /locus_tag="VF686_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8660676.1"
                     /translation="MRIAPHALTAVAAISVALLAGRVYQPAKAEEAPPLTQIQISALA
                     DQAFAAAGTPAGLTTPEAIPVQIRRGETFEQAVRRTGIAPEEASAVAATLSNAMDVTS
                     MRAGQKFETAISQPRGGRGNPRLIGLTMRTGPATQLTVSRSFDGALRLRALEEKVTHE
                     TVVLTGKVEGSLSRTARREGAPADVARVAGRLFSHKFDMDRDIQSDDQFTYVFGRTVT
                     ENGRTIGTDGLLYAELKGVAFYRFQPTGAKEAQYFDATGKNTRSAFMRTPLERGFRVS
                     SSFGFRRHPIAGYRKMHQGIDFAAGMGTPVVAPADGVVVEARRWGGYGNWLRIRHANG
                     LDSGYGHLSRYASGVRAGQRVRQGQIVAYVGSTGASTGPHLHYELWRSGQRINPAGVR
                     TTEGTILAGADLAAFKAEKARIDRIRAAGGERRPVGGQRAVAAAPAPAVLRPARG"
     gene            3008..3898
                     /locus_tag="VF686_02335"
     CDS             3008..3898
                     /locus_tag="VF686_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8660677.1"
                     /translation="MEQRRQFLTGLAAGAALWSAGSGAWAQTAFRSPRITVTTRGRGP
                     DVILIPGLASTAEVWAGLAGQLEGRRRLHLVSVRGFGDLAAEGNADGSVMPGVANEVR
                     RYMTEQRLERPAVIGHSMGGQVGIRVAADAGGRVGQVMAVDSSPFFAALISPAATVGD
                     VEPIAQLAYQAIQFLGDEALKTRGRSMGLELGGAADAVFGSMGWQGGDRRVLAQSLYE
                     VMTVDLRRRLPDITAPVTVVYGWSSDQNSPRARADALFRGAYARLRRPATFERIEGAE
                     HMVMIDQPRRFLAAVERFLA"
     gene            complement(3895..4527)
                     /gene="maiA"
                     /locus_tag="VF686_02340"
     CDS             complement(3895..4527)
                     /gene="maiA"
                     /locus_tag="VF686_02340"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX8660678.1"
                     /translation="MILHGYWRSGAAYRTRIALNLKGVAYDQQGVDLRTGAQRSEAFV
                     ALNPQGMVPALEVDGAVLTQSPAILEWLEETHPQPPLLPANPVGRAHVRAMAALIGCD
                     IHPLNNLRVGKALREIFGADQAAVDAWAARWILPGFTALESLVGRHGEGWCFGAAPTL
                     ADCYLIPQIYSGRRFNVALDAFPRLLAIDEAAKQHPAFAAAHPDVQPDAD"
     gene            complement(4524..5177)
                     /locus_tag="VF686_02345"
     CDS             complement(4524..5177)
                     /locus_tag="VF686_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8660679.1"
                     /translation="MTDWLFDPAPTPSLPIHGSAQRFPIRRIFCVGQNYAAHAKEMGH
                     ERAEPFFFTKPADAVVVEGADPVFPTATANLHHEVELVCAVGADGTIAGWAVGCDLTR
                     RDLQAAAKEKGRPWDAAKGFDQSAPCGPITLGALPAPAAPIALSVNGEARQSGRLDDM
                     VWNPEEILAKARTLWDVRPGDLIFTGTPEGVGPLQPGDRVEASIGGLQPLAFTVTPR"
     gene            5315..5695
                     /locus_tag="VF686_02350"
     CDS             5315..5695
                     /locus_tag="VF686_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEX8660680.1"
                     /translation="MATTKAARTAVKNDLNTLKDDLKVLGAEEKARLREKATAAETRM
                     RAKAAETEARLREQSDVLRERARGYYDTARVRGQEYYDEAQERFDEAQRYITERVQER
                     PVQSTAIALGVGVVLGLLLAGRRR"
     gene            5695..>6089
                     /locus_tag="VF686_02355"
     CDS             5695..>6089
                     /locus_tag="VF686_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660681.1"
                     /translation="MFGGGLARNLVKKAVAASVVAGAGFVAILALGATIFFALQLVVV
                     PLAAAAITFLLFAIVAAVVAMVFLARSEEEEDYEDEPSGLGARAYSLFKQRPILGTVA
                     ALAGGWIFLRNPALATMVAAAFTEKPHGR"
BASE COUNT          901 a         2050 c         2209 g          929 t
ORIGIN      
        1 tcgagggcgg cgttgcggcc gccgagggtg aagccggccg atcgcagggt cacgccggtc
       61 ccctcaaggc cggcgccggt ggcccgggcg gccatcacgg tcgccggacc ctgcagtctc
      121 cagccgaaac ccttggcgtc ccggctgaag gccgtgtcgg cgtggtcgag ccgcagacgg
      181 atgcctgacg cggccgtgcc cggaccgtcc acgcgcgcgg cgcgcacgtc gaggtcgccg
      241 tcgcccgcga tacggaaggt ttcgatccag cccgtcgtct ggccggtgaa gccgaggcgg
      301 gcgtctgtcc cccgggccgt ggttccgccc gacgaaaggc gagccgcggt gaaggcggcg
      361 ttgatggcgg cccggccgtc gccccggcgc cccttcaggt cgggataggg caggttgccg
      421 gtgacgacga gccgggccgt atctccggcg aggccctcca tggtcgcgtg ctcggcggtg
      481 gcgtcgaagc gcacggcgat gcggtcgccg gtagtggtca ggtcgacggc ggcgctcagg
      541 ccccgcgcct cggcctcgcc gctcttgaag gcggtggccg gcattcgggc gaccagccgc
      601 atcagcttgc cgttgtcgac gcgggcgtcg ccgaggatgt tggccgggcc gtagtcggtg
      661 tcgagtcgca cccgggcgcg ttcgacgatg accagaggcg accggctgtc gggacgcggc
      721 gggcggccgg tgaactcctc gatcagcgga tccagcgagc cgaaggtcag cttgccgccg
      781 cgcacgctgg cgcggaccac ggggcggacc aggcggatgc gggtcggggt gacgccgagc
      841 ccgcccttcg accatggcgc gccgatgccg tagtcgacct cgacgcgttc gatcaggacg
      901 tcgggattgg ccgggtcgcc gatacggatg cgggcgacga ggccgtccat ctcgagccgc
      961 tcgatatcca tgtcggcgtc gacgccccgc tgctcaagcc agccgatcag gatctgacgg
     1021 gtggcggcgc ggcggttgag ccacaccacc gcgaacagga tcagcaggac gcagagcacg
     1081 gcgccggcca cgatcaggat gcggcggccg ccggtgcggc ggggacgccg gggaacgggg
     1141 gcgggcgtgg cgtcggcgct catacggggg tcatagccgg acggaggccc gtgcggaaag
     1201 cccgctgaac aaaaccgtaa acttgtcgaa tactacagcc gtgtctatcg aagcacagtt
     1261 gagtggcgca taggccactt tgaatgcatc ctctctcgct ctgagatggc tgatacacgt
     1321 cccgagcgac tccggttcga aggcctgcga atacactgtt caactcgcac gatattctca
     1381 tggttcacgg aggctttacc gaggataacg gctccggtta acggtctgta acgatccttc
     1441 ttcccatcat gggactgacc ggctatatgg cctgtctcgc gaaggggggt ggctccggcg
     1501 cgatccttgc ttcagtagcc tggcgcccaa cggcgtcgtc gtttttcggg gaccgatggc
     1561 tcagttcgat ccacgtcgcc aaccgatgcg catcgcgcct cacgccctga cggcggtggc
     1621 ggcgatctcg gtggctcttc ttgccggccg cgtataccaa cccgccaaag cggaagaggc
     1681 tccgcctctg actcaaattc agatttccgc cctcgccgac caggcgttcg ccgccgccgg
     1741 aaccccggcc ggcctgacca cgccggaggc cattcccgtc cagatccgcc gcggcgagac
     1801 cttcgagcag gccgtgcgcc gcaccggcat cgcgccggaa gaagcctcgg ccgtcgccgc
     1861 caccctgtcg aacgccatgg acgtgacgtc gatgcgggcg ggccagaagt tcgagaccgc
     1921 catttcccag ccgcgcggcg gccggggcaa tccgcgcctg atcggcctga cgatgcgtac
     1981 aggtccggca acccagctga ccgtctcgcg cagcttcgac ggcgccctgc gcctgcgcgc
     2041 gctggaagag aaggtcacgc acgagaccgt ggtcctgacc ggcaaggtcg agggctcgct
     2101 gtcgcgcacg gcgcggcgcg aaggcgcccc ggccgacgta gcccgcgtcg ccggccgcct
     2161 gttctcgcac aagttcgaca tggaccggga catccagtcc gacgaccagt tcacctacgt
     2221 cttcggccgc accgtcaccg agaacggccg caccatcggc accgacggcc tgctctacgc
     2281 ggaactgaag ggcgtggcct tctatcgctt ccagccgacc ggcgcgaagg aagcgcaata
     2341 tttcgacgcc accggcaaga acacccgttc ggccttcatg cgcacgccgc tggagcgggg
     2401 cttccgcgtc tcgtcctcgt tcggcttccg ccgtcacccg atcgccggct atcgcaagat
     2461 gcaccagggc atcgacttcg ccgcgggcat gggcacgccg gtcgtggccc ccgccgacgg
     2521 cgtggtcgtc gaagcccgcc gctggggcgg ctacggcaac tggctgcgca ttcgtcacgc
     2581 caacggtctg gatagcggct acggccacct gtcgcgctac gcctcgggcg tccgcgccgg
     2641 ccagcgggtc cgtcagggcc agatcgtcgc ctatgtcggc tcgaccggcg cctcgaccgg
     2701 tccgcacctg cactatgaac tgtggcgcag cggccagcgg atcaatccgg cgggcgttcg
     2761 caccaccgag ggcaccatcc tggccggggc cgatctggcc gcgttcaagg ccgagaaggc
     2821 ccgcatcgac cgcatccggg ccgccggcgg cgaacgccgc ccggtgggtg gccagcgcgc
     2881 cgtcgccgct gctccggccc ccgccgtcct gcgtccggcc cggggctagc gcggctttca
     2941 gcggccgacg cttcatcgtc atgccgtaat ctagaaccac gctgcttgcg aagcattgtg
     3001 gaacggcatg gaacaaagac gacaattcct gacggggctg gccgctggcg cggcgctgtg
     3061 gagcgccggt tcgggcgcgt gggcgcagac ggcgttccgg tcgccgcgca tcacggtgac
     3121 gacgcgcggg cgcggccccg acgtcatact gatccccggt ctggcctcga ccgccgaggt
     3181 gtgggccggg cttgcgggcc agctcgaagg tcggcggcgg ctgcatctgg tctcggtgcg
     3241 cggcttcggc gatctggcgg ccgagggcaa tgccgacgga tcggtcatgc ccggggtggc
     3301 caatgaggtg cggcgctaca tgaccgagca gcggctggag cggcctgcgg tcatcggcca
     3361 ttcgatgggc ggacaggtcg gcattcgcgt cgccgccgac gccggcggtc gggtgggcca
     3421 ggtgatggcg gtggattcct cgcccttttt cgcggccctg atcagtccgg cggcgacggt
     3481 cggcgacgtg gagccgatcg cccagctggc ctatcaggcg atccagttcc tcggcgacga
     3541 ggcgctgaag acgcgcggcc ggtcgatggg gctggagctg ggcggcgccg ccgacgccgt
     3601 attcggatcc atgggctggc agggcggcga ccgccgggtg ctggcccagt cgctgtacga
     3661 ggtgatgacg gtcgacctgc gccgccgcct gcccgacatc acagctccgg tgacggtagt
     3721 ctatggctgg agctcggacc agaattcacc acgcgcccgg gccgacgccc tgttccgagg
     3781 ggcctacgcc cgcctgcgcc ggcccgccac cttcgaacgg atcgaaggcg ccgagcatat
     3841 ggtgatgatc gaccagccga ggcggttcct cgccgcggtg gagcggtttc tggcctagtc
     3901 ggcgtcgggc tggacgtctg gatgggcggc ggcgaaggcg gggtgctgtt tcgccgcctc
     3961 gtcgatggcc agcaggcgcg ggaaggcgtc gagggcgacg ttgaagcgcc ggcccgaata
     4021 gatctgcggg atcaggtagc agtccgccaa ggtcggggcg gcgccgaagc accagccctc
     4081 gccatgccgg ccgaccagcg attccaaggc agtgaagccg ggcaggatcc agcgcgcggc
     4141 ccaggcgtcg acggcggcct gatcggcgcc gaagatttcg cgcagggcct tgccgacgcg
     4201 cagattgttg agcggatgga tatcgcagcc gatcagggcg gccatggcgc ggacatgggc
     4261 gcggccgacg ggattcgcgg gcagcagcgg cggctgcgga tgcgtttctt ccagccattc
     4321 gagaatggcc gggctctggg tcaggacggc gccgtcgact tccagcgcgg gcaccatgcc
     4381 ctgcgggttc agggcgacga aggcctctga ccgctgggcg ccggtgcgca gatcgacgcc
     4441 ctgctggtcg taggcaacgc ccttgaggtt cagagcgatg cgggtgcggt aggccgcgcc
     4501 cgagcgccag tagccgtgca ggatcatcgc ggcgtcaccg tgaaggccag cggctgcagg
     4561 ccgccgatgg aggcctcgac ccggtccccg ggctgcagcg ggccgacgcc ctccggcgtg
     4621 cccgtgaaga tcaggtcgcc gggccgcacg tcccagaggg tgcgcgcctt ggcgagaatt
     4681 tcctccgggt tccagaccat gtcgtccagc cgacccgact ggcgcgcctc gccgttgacc
     4741 gacagggcga tgggggcggc gggggccggc agggcgccga gggtgatcgg gccgcagggg
     4801 gccgactggt cgaacccctt ggccgcgtcc cacggacggc ccttttcctt ggccgcggcc
     4861 tggaggtcgc ggcgggtcag gtcgcagccg acggcccagc cggcgatcgt cccgtccgcg
     4921 ccgacggcgc agaccagttc gacctcgtga tgcagattgg ccgtggcggt cggaaagacg
     4981 ggatcggcgc cctcgacgac cacggcgtcg gcgggcttgg tgaagaagaa gggctcggcc
     5041 ctttcgtggc ccatctcctt cgcgtgggcg gcgtagttct ggccgacgca gaagatgcgc
     5101 cgtatcggaa agcgttgcgc gcttccgtgg atcggcaggg agggggtcgg agcgggatca
     5161 aacagccagt cggtcatatg gtctgtctat gcgcggacgc cgtgcttcgc cagccgtccg
     5221 aaatggtgca atgcgggaac ctgcgccatg cggtggcgtt ccggctttga attctctatc
     5281 taacagccaa gctttaaaca tcggagcgcg catcatggcc acgaccaagg cggcacggac
     5341 tgccgtgaag aacgatctca acactctcaa ggacgacctg aaggtcctcg gcgccgagga
     5401 aaaagcgcgt ctgcgcgaaa aggcgacggc tgcggaaacc cgcatgcgcg ccaaggccgc
     5461 cgagaccgag gcccgtctgc gtgaacagtc ggacgtcctg cgcgagcggg cccgcggcta
     5521 ttacgacacc gcccgcgtgc gcggtcagga atactatgac gaggcgcagg agcgcttcga
     5581 cgaagcccag cgctacatca ccgagcgggt gcaggagcgt ccggtgcaat cgaccgccat
     5641 tgcccttggg gtcggcgtcg tgctgggtct gctgctcgcg ggccgtcgcc gctgatgttc
     5701 ggcgggggac tggccaggaa tctggtcaag aaagccgtcg cggcttcggt cgtggcgggc
     5761 gccggcttcg tggccattct ggcgttgggg gcgacgatct tcttcgccct gcagctggtc
     5821 gtcgtgccgc tggccgcggc ggccatcacc ttcctgctgt tcgccatcgt ggccgcggtg
     5881 gtggcgatgg tcttcctcgc ccgcagcgag gaagaggaag actatgagga cgagccctcc
     5941 ggtctgggcg cgcgcgccta cagcctgttc aagcaacgcc ccattctggg gactgtcgcg
     6001 gcgctggcgg ggggatggat cttcctcagg aacccggcgc tggcgacgat ggtcgcggcc
     6061 gccttcaccg agaagccaca cgggcgcag
//
LOCUS       DASVVN010000154         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_400353,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000154 DASVVN010000000
VERSION     DASVVN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400353"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF686_02360"
     CDS             <1..816
                     /locus_tag="VF686_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8660682.1"
                     /translation="SLAILLAGVAAPALAQTAPDWSGPYIGVYGGALENNEDAGESLI
                     FDRDFDGQFDDTVVLSGTTNSAFSPGSCDGAATAQAAGSGCDVDPTGVEGSIRAGYDM
                     QFGPLVVGLVGELSAVDLEDSVTSFSTTPAAYVFTRQVEQMAAIRARVGFPVGPALLY
                     VTGGAAKAKVENSFRTTNTANSFVATVDEDDADGYQLGGGVEWRLAPNLSVTGEYLYS
                     NLQPGDYVVRVGPGTAGPTNPFILPPNTAGTDMTRSNDGLELHALRIGMNVRF"
     gene            1017..>4303
                     /locus_tag="VF686_02365"
     CDS             1017..>4303
                     /locus_tag="VF686_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX8660683.1"
                     /translation="MPGEPASKLPQAITLEALETRFRKALTGKPDARALDFLAQAFED
                     YAADETPELGGDDLAVLLAGVWKAAEGRAHGEPARITVSPVTGADGRAAGYDLLTVIQ
                     DDRPFLVDSVMGELAEAGVTVRSLYHPVSDEDGGRESIIVVFIDPLPQERRDALGEEL
                     SVTLADVAGAVNDHAAMAGLMARSIEHLEGGPKGVAPEVLAENLEFLRWLQQDHFVFL
                     GARDYDYPRLKDGGYEAEAPLSQSGAGLGVLADPNRTVLRRANEPAVLTRQMKKQLDL
                     SEPVTVAKANVRSRVHRRAYMDYVGVKRFGPDARPSGETRFVGLFTAEAYDRAAAEVP
                     LIRRKVAGALARGGKIEGSHSHKRLKNILENYPRDELFQIDEDELLDISLGILHLHDR
                     PRIKIFTRKDPFDRFVSVLAFIPRERFDASVRERIGGILAKAWGGRLSAWYPQLSDQP
                     LVRIHYIIGVTPGDHPCPDHAELEAQVAEAGRSWIDRFEGALRAADVDDVAVGPLSVR
                     WAEAFGAGYRDRYDADEAVRDLMAVDGLNSSGQNDGGDPVSVRAFRTEADGPLNFRFK
                     LYRRGAAVPLSDVLPILADMGLKTLEEWGHALRPTGDPEIHVHEFLLEDPRGGDLKFE
                     DVKGPFEEAFSAVWTGLTESDGFNRLVLELGVNWREAALVRTLARYRQQTGLDPSQAV
                     QEEALRDYPAMARAILELFESKFDPAQGGDGASREAVVEKQVAGINALLQDVKSLDHD
                     RALRRMALLVQAIKRTNYYQTGADGRPRPHISIKIASRELADLPLPKPFREIFVWAPH
                     IEGVHLRFGPVARGGLRWSDRRDDFRTEVLGLVKAQQVKNAVIVPVGSKGGFFPKHLA
                     AIVRAGGDRDAQQAEAIRAYKTFLSGLLDVTDNINAKGKVVPPQHVIRFEGDDPYLVV
                     AADKGTATFSDIANGVSRDYGFWLDDAFASGGSVGYDHKVMGITARGAWEAVKRHFRE
                     MGKDIQSQPFTVVGVGDMSGDVFGNGMLLSQQIRLVAAFDHRDIFIDPNPDPATSFAE
                     RKRLFELPRSSWQDYDKTKISEGGGVYSRAAKSIALSAQARALLQLDVEAPTPAEVMR
                     AILKADA"
BASE COUNT          682 a         1488 c         1481 g          652 t
ORIGIN      
        1 tctctggcga tcctgctggc cggcgtcgcc gcgccggccc tggctcaaac cgctcccgac
       61 tggagcggcc cgtatatcgg cgtctatggc ggcgctctgg aaaacaacga ggacgccggc
      121 gaaagcctga tcttcgaccg cgatttcgac ggccagttcg acgataccgt ggttctgagc
      181 ggcacgacta acagcgcatt cagccccggc tcgtgcgacg gggcggcgac ggcccaggcc
      241 gcgggaagcg gttgcgatgt cgatccgacg ggcgtcgagg gctcgatccg cgccggctac
      301 gacatgcagt tcggcccgct ggttgtcgga ctggtcggcg aactgagcgc cgtggatctg
      361 gaagactcgg tcacctcgtt cagcaccacc ccggccgcct atgtcttcac ccgtcaggtg
      421 gagcagatgg ccgcgatccg cgcccgcgtc ggcttccccg tcggcccggc cctgctgtac
      481 gtcaccggcg gcgcggccaa ggccaaggtc gagaacagct tccgcaccac caacacggcc
      541 aacagcttcg tcgcgaccgt cgatgaggac gacgccgacg gctatcagct gggcggcggc
      601 gtcgagtggc ggctggctcc gaacctgtcg gtgacgggcg agtatctcta ttcgaacctg
      661 cagccgggcg attatgtcgt ccgcgtcggt ccgggcacgg cgggcccgac caaccccttc
      721 atcctgccgc ccaacacggc gggcaccgac atgacgcgca gcaacgacgg ccttgagctg
      781 cacgcgctac gtatcggcat gaacgtccgc ttctaggcga accacgtcga tcgctgatcg
      841 gcgacgccgg ctccggaaac ggggccggcg ttgtcgtttt gggggttccc gagacgggcc
      901 ggctggccta gtcaggcggc ccatcggaca gcgcccttgc ccccgtggcg aagcggccta
      961 gtctggcccc tcatccggcg gccgatgcag aggccgcgcc tgcaagagac ctgcccatgc
     1021 ccggcgaacc ggcttcgaag cttcctcagg cgatcacgct ggaggcgctg gagacgcggt
     1081 tccgcaaggc cctgacaggc aagcccgacg cccgcgccct ggacttcctg gcccaggcct
     1141 tcgaggacta tgcggcggac gaaacccccg aactgggcgg cgacgacctg gccgtcctgc
     1201 tggccggcgt gtggaaggcg gccgagggcc gcgcccatgg cgaaccggcg cggatcaccg
     1261 tctcgccggt gacgggcgcc gacgggcgcg cggcgggcta tgacctgctg accgtcattc
     1321 aggacgaccg gcccttcctc gtcgacagcg tcatgggcga gctggccgag gccggggtga
     1381 ccgtgcgctc gctgtaccac ccggtcagcg acgaggacgg cgggcgggaa tccatcatcg
     1441 tcgtgttcat cgaccccctg ccccaggagc gccgcgacgc cctcggtgag gagctgtccg
     1501 tgacgctggc cgacgtggcc ggggccgtga acgatcatgc cgcgatggcg ggcctgatgg
     1561 cgcgctcgat cgaacatctg gagggcggac ccaagggcgt ggcccccgag gtgctggccg
     1621 aaaatctgga gttcctgcgc tggctgcagc aggaccattt cgtcttcctc ggcgcgcggg
     1681 actacgatta tccgcgcctg aaggacggcg gctacgaggc cgaggccccg ctgtcccagt
     1741 cgggcgccgg tctgggcgtg ctggccgatc cgaaccggac cgtgctgcgc cgcgccaacg
     1801 agccggcggt gctgacgaga cagatgaaga agcagctgga cctgtccgag ccggtgacgg
     1861 tggccaaggc caatgtccgg tcgcgggtcc atcgccgcgc ctatatggac tacgtcgggg
     1921 tcaagcggtt cgggccggac gcgcgtccgt cgggcgagac gcgcttcgtc ggcctgttca
     1981 ccgccgaggc ctatgaccgg gccgccgcgg aggtgccgct gatccggcgc aaggtggccg
     2041 gcgccttggc gcgcggcggc aagatcgagg gcagccacag ccacaagcgg ctgaagaaca
     2101 tcctcgaaaa ctacccccgc gacgaactct tccagatcga cgaggacgag ctgctggata
     2161 tctcgctggg catcctgcac ctgcacgacc gcccgcggat caagatcttc acccgcaagg
     2221 atccgttcga ccgcttcgtc tcggtgctgg ccttcatccc gcgcgagcga ttcgacgcct
     2281 cggtccgtga acggatcggc ggcatcctgg ccaaggcctg gggcggtcgt ctgtcggcct
     2341 ggtatccgca gctgtcggat cagccgctgg tgcgcatcca ctatatcatc ggcgtcacgc
     2401 cgggcgatca tccgtgcccg gatcatgccg agcttgaggc ccaggtggcc gaggccggcc
     2461 gcagctggat cgaccggttc gaaggcgccc tgcgcgccgc cgatgtggac gacgtcgccg
     2521 tcggcccgct cagcgtgcgc tgggccgagg ccttcggcgc cggctaccgc gatcgctatg
     2581 acgccgacga ggccgtgcgc gacctgatgg ccgtcgacgg cctgaacagc agcggccaga
     2641 acgacggcgg cgaccctgtt tcggtccggg ctttccgcac cgaagccgac ggaccgctga
     2701 atttccgctt caaactgtat cgtcgcggcg cggccgtgcc gctatcggac gtgctgccga
     2761 tcctcgccga catgggactg aagacgctgg aggaatgggg ccacgccctg cgccccaccg
     2821 gcgatccgga aatccacgtg cacgagttcc tgctcgagga tccgcgcggc ggcgatctga
     2881 agttcgagga cgtgaagggc ccgttcgagg aagccttctc ggccgtctgg accggactga
     2941 ccgagagcga cggcttcaat cgtctggtgc tggaactggg cgtcaactgg cgcgaggcgg
     3001 cgctggtgcg caccctcgcc cgctatcgcc agcagacggg tctcgatccg tcgcaggcgg
     3061 tgcaggaaga ggcgctgcgc gactatccgg ccatggcgcg cgccatcctg gagctgttcg
     3121 agagcaaatt cgatccggcc cagggcggcg acggcgccag ccgcgaggct gtggtcgaga
     3181 aacaggtcgc cggcatcaac gccctgctgc aggacgtgaa gagcctggac cacgaccggg
     3241 cgctgcgccg gatggccctg ctggtgcagg cgatcaagcg gaccaactac tatcagacgg
     3301 gcgccgacgg ccggccccgg ccgcatatct cgatcaagat cgcctcgcgc gaactggccg
     3361 acctgcccct gcccaagccg ttccgcgaga tcttcgtctg ggccccgcat atcgagggcg
     3421 tccacctgcg cttcggcccc gtggcgcggg gcggcctgcg ctggtccgac cgccgcgacg
     3481 acttccgcac agaggtgctg ggtctggtca aggcgcagca agtcaagaac gcggtcatcg
     3541 tgccggtcgg ctccaagggc ggcttcttcc ccaaacatct ggccgcgata gtccgcgccg
     3601 gcggggaccg cgacgcccag caggccgagg ccatccgggc gtacaagacc ttcctgtcgg
     3661 gtctgctgga cgtcaccgac aacatcaacg ccaagggcaa ggtcgtcccg ccgcagcatg
     3721 tgatccgctt cgagggcgac gacccctatc tggtcgtggc tgccgacaag ggtacggcga
     3781 ccttctccga catcgccaac ggcgtgtcgc gagactacgg cttctggctg gacgacgcct
     3841 tcgcctcggg cggctcggtc ggttacgacc acaaggtcat gggcatcacg gcccgcggcg
     3901 cctgggaggc ggtcaagcgc cacttccgcg agatgggcaa ggacatccag agccagccct
     3961 tcaccgtcgt cggcgtcggc gacatgagcg gcgacgtgtt cggcaacggc atgctgctgt
     4021 cgcaacagat ccggctggtg gccgcctttg accaccgcga catcttcatc gacccgaacc
     4081 cggacccggc gacgtcgttc gccgagcgca agcggctgtt cgagctgccg cgctcctcct
     4141 ggcaggacta cgacaagacc aagatctcag aaggcggcgg cgtctattcg cgggcggcca
     4201 agagcatcgc gctgtcggcg caggcccgcg cgctgctgca gctcgatgtg gaggcgccga
     4261 cgccggctga ggtgatgcgc gccatcctaa aggccgacgc cga
//
LOCUS       DASVVN010000155         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_505425,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000155 DASVVN010000000
VERSION     DASVVN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505425"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VF686_02370"
     CDS             <1..446
                     /locus_tag="VF686_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX8660684.1"
                     /translation="EAVTEGEDVTRGIDEAATGSKTTETIAERIRVLQRQQGGSLAQK
                     LDIERQLADLQRQYDAQQIELRELNARVQSARLTIDYREAGVMAADSPTRPVARALQN
                     AFGISMGMLAALITAGSIMLPVAVIGGAVWWATVRRRRKLAEAAA"
     gene            complement(443..1321)
                     /locus_tag="VF686_02375"
     CDS             complement(443..1321)
                     /locus_tag="VF686_02375"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX8660685.1"
                     /translation="MAFVPFDDRDGWIWMDGDFVPWREAKTHVLTHALHYGSSVFEGE
                     RMYGGEIFKLTPHSERLKRSANLLDFDLPYSVAEIDAACKETCEKMGLSDCYVRPVAY
                     RGPEQVSVSAANNKTHVAIAVWEWPSYFDAATKAKGIRLEWAKWRRPDPMTAPSAAKA
                     AGLYMICTMSKTAAEKRGYSDAMMLDWRGYVAEATGANVFFVQDGVLHTPPVDAILDG
                     ITRQTIIEMAAAKGIEVVVRHIRPEELATFSECFVTGTAAEVTPVCEIGEYRFTPGAL
                     SLGLMEDYGRLVRGQI"
     gene            1410..2141
                     /locus_tag="VF686_02380"
     CDS             1410..2141
                     /locus_tag="VF686_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8660686.1"
                     /translation="MSDVRFHGRSGPVARPGAGRHLLVVDDDDRIRELLKEFLAREGY
                     RVTGAAHAAAAKRLMELIEFDLVVLDVMMPGESGFDLTTWVRSKAETSKTPVLLLTAR
                     GDPDDRIEGLSRGADDYMSKPFDPRELALRVDAILRRTGGKPLTPREIKLGPAVFDME
                     RLELSKDGKPQRLTEAEAQLLKTLAIHAHSAVERMSLSPDTADITGRAVDVQVTRLRR
                     KLEADPKNPRYLQTVRGVGYMLAPD"
     gene            2149..3492
                     /locus_tag="VF686_02385"
     CDS             2149..3492
                     /locus_tag="VF686_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660687.1"
                     /translation="MRLLPASLWPRFLKRRLPTSLWGRSLLIIVLPVLVMQVAVTWAF
                     FDMHWQTVTARLSDGLAGDIAWAAESWRDDPTPRNMAVISERAQRSMSLSIALQDGAT
                     LPDENRRGPVGVVDRTLEKALAARLDQPFWFDTTRYPAYVDIRVQQPQGVLRIIAPRE
                     RAVATQAHIFVLWLLIATVLLMGVAILFIRNQVRAIERLAEAAEAFGRGETQERFKPH
                     GAREVRAAAVAFLAMRERIQRHIDQRTALLASVSHDLRTPLTRLRLELALAPPFKRAG
                     AMQGDLDEMEHMIDEYLAFARGEAGEAAQPVFLSDLLAAAGEDARRAGAEVEVVAPEG
                     LTASLRPLGFKRALTNLAGNAAAHGDRVRLSARALPSGGLEIAVEDDGPGIPEDMHEE
                     AFRPFSRLDASRNQNHKGVGLGLAIARDVARGHGGDITLERSDMGGLRALIRLPG"
     gene            3572..4066
                     /locus_tag="VF686_02390"
     CDS             3572..4066
                     /locus_tag="VF686_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660688.1"
                     /translation="MTRFALLAPLAAGLALLAAPALADPASVNITLGPDVLDAADELG
                     EREVQAQASRLADVVRQTLARRGALDGAEINLTLTDLKPNRPTMQQMIDRPGLDGIRS
                     ISTGGATVTGHVTTADGQIHPVAYDWYSSNITDVRGFSAWQDADRVYRRLADNLAEGR
                     YKGR"
BASE COUNT          658 a         1487 c         1377 g          644 t
ORIGIN      
        1 aggaggccgt caccgagggc gaagacgtca cgcgcggcat cgacgaggcc gccaccggct
       61 ccaagaccac cgaaaccatc gccgagcgca tccgcgtcct gcagcggcag cagggcgggt
      121 ccttggccca gaagctcgac atcgagcggc agctggccga cctgcagcgc cagtacgacg
      181 cccagcagat cgagttgcgc gagctgaacg cccgcgttca gtccgcccgg ttgacgatcg
      241 actaccggga ggctggcgtc atggccgccg acagcccgac ccgcccggtg gccagggcgc
      301 ttcagaacgc cttcggcatc tccatgggca tgctggcggc gctgatcacc gccggttcga
      361 tcatgctgcc ggtcgccgtc atcggcggcg cggtgtggtg ggccacggtg cgccgtcgcc
      421 ggaaactggc cgaggctgcc gcctagatct gaccgcggac cagccggccg tagtcttcca
      481 tcaggccgag gctgagggcg ccgggggtga atcggtattc gccgatctcg cacaccggcg
      541 tcacctcggc ggccgtgccg gtgacgaaac attcgctgaa ggtcgccagc tcctcgggcc
      601 ggatgtggcg tacgaccacc tcgatgccct tggcggcggc catctcgatg atggtctggc
      661 gggtgatgcc gtccaggatc gcatcgaccg ggggggtgtg cagaacgccg tcctgaacga
      721 agaagacgtt ggcgcccgtg gcctccgcca catagccgcg ccagtccagc atcatggcgt
      781 cggaatagcc gcgcttctcg gccgctgtct tggacatggt gcagatcatg tacaggcccg
      841 ccgccttggc ggccgacggc gccgtcatcg ggtcgggccg gcgccatttg gcccactcca
      901 gccgaatccc cttcgccttg gtcgcagcgt cgaaatagct gggccactcc cacacggcga
      961 tggccacatg ggtcttgttg ttggccgccg agacgctgac ctgttccggc ccacggtagg
     1021 ccaccggccg cacatagcag tcggaaaggc ccatcttttc gcaggtttcc ttgcaggccg
     1081 cgtcgatctc ggccacgcta tacggaaggt cgaaatcaag caggtttgca gaacgcttca
     1141 ggcgttcgct atgcggggtc agcttgaaga tttcgccgcc gtacatacgc tcaccctcga
     1201 acaccgacga gccgtagtga agggcatggg tcagaacgtg cgttttcgcc tcccgccagg
     1261 gtacgaaatc gccgtccatc cagatccagc cgtcacgatc gtcgaaagga acgaaggcca
     1321 ttgaataacg tctcccgcga agtcacccgg gttagagccc tccgaaccgc tcaggtcaac
     1381 gcctggaacg gcgtccggat cacgtccgca tgagcgacgt gcgtttccat ggccgatccg
     1441 gcccggtcgc ccgccccggc gccggccggc atctgctggt cgtcgatgac gacgaccgca
     1501 tccgcgagct gctgaaggaa ttcctcgccc gcgaaggcta ccgcgtcacc ggcgccgccc
     1561 acgcggccgc ggcgaagcgg ctgatggagc tgatcgagtt cgacctggtc gtgctggacg
     1621 tgatgatgcc gggcgaaagc ggtttcgacc tgacgacatg ggtgcggtcc aaggccgaga
     1681 cctcgaagac gccggtcctc ctgctgaccg cgcgcggaga tccggacgac cggatcgaag
     1741 gcctgtctcg cggcgccgac gactatatgt ccaagccttt cgaccctcgc gagctggcgc
     1801 tgcgcgtcga cgccatcctg cgcaggacgg gcggcaagcc gctgaccccg cgcgagatca
     1861 agctcgggcc ggcggtattc gacatggagc ggctggagct gtcgaaggac ggcaagcccc
     1921 agcgcctgac cgaggccgag gcccagcttc tcaagacgct ggccatccac gcccattccg
     1981 cggtcgagcg catgagcctg tcgcccgata ccgccgacat caccggccgc gccgtggacg
     2041 ttcaggtcac ccgcctgcgc cgcaagctgg aggccgaccc caagaacccg cgctacctcc
     2101 agaccgtgcg cggcgtcggc tatatgctgg ccccggactg atccggcgat gaggctgctt
     2161 cccgccagcc tctggccgcg gttcctgaag cggcgtctgc cgacctcgtt gtggggccgg
     2221 tcgctgctga tcatcgtcct gcccgtgctg gtcatgcagg tagccgtcac ctgggcgttc
     2281 ttcgacatgc actggcagac ggtgacggcc cgcctgtcgg acggactggc cggcgacatc
     2341 gcctgggcgg ccgaaagctg gcgcgacgat cccacgcccc ggaacatggc ggtcatctcc
     2401 gaacgggccc agcgatcgat gtccctgtcc atcgccctgc aggacggcgc caccctgccc
     2461 gacgagaacc gccgcggccc ggtgggggtc gtggaccgca ccctggagaa ggctctggcc
     2521 gcccggctcg accagccttt ctggttcgac accacccgct accccgccta tgtggacatc
     2581 cgggtgcagc agccgcaggg ggtgctgcgg atcatcgccc cgcgcgagcg cgcggtcgcc
     2641 acccaggccc atatcttcgt cctgtggctg ctgatcgcga ccgtcctgct gatgggcgtc
     2701 gccatcctgt tcatccgcaa tcaggtgcgg gccatcgaac ggctggccga ggcggcggaa
     2761 gccttcggcc ggggcgaaac ccaggaacgg ttcaagccgc acggcgcccg cgaggtccgc
     2821 gccgccgccg tcgccttcct ggccatgcgc gaacggatcc agcgccatat cgatcagcgc
     2881 accgccctgc tggcctcggt cagccacgac ctgcgcacgc ccctgacccg gctgcgtctc
     2941 gaactggcgc tggcgccccc gttcaaacgc gccggggcga tgcagggcga tctggacgag
     3001 atggagcata tgatcgacga atacctcgcc ttcgcccgcg gcgaggccgg cgaggcggcg
     3061 cagccggtct tcctgtccga tcttctggcc gccgccggcg aggacgcccg gcgcgccggg
     3121 gccgaggtcg aggtcgtcgc gcccgagggg ctgacggcat cgcttcgtcc cctcggcttc
     3181 aaacgggccc tgaccaatct ggctggcaat gcggccgccc acggcgaccg cgtcaggctc
     3241 agcgcccgcg ccctgccgtc aggcgggctg gagatcgcgg tcgaggatga cggccccgga
     3301 atccccgagg acatgcacga ggaagccttc cggcccttct cgcgtctcga cgcctcgcgg
     3361 aaccagaacc acaagggggt cgggctgggg ttggccatcg cacgcgacgt cgcgcgaggc
     3421 cacggcggcg acatcactct ggaacgcagc gatatgggcg ggcttcgggc cctgatccgc
     3481 ctgccgggct gatcaaattt cgcggcgccg ctggtaaagc gtggccttaa ggatcatctt
     3541 tcgtctgacc ggggggtcag tgaggatcaa catgaccaga ttcgctctcc ttgcaccgtt
     3601 ggccgccggc ctggccctgc ttgccgcacc cgccctggcc gaccccgcct ccgtcaacat
     3661 cactctcggc cccgacgtgc tcgacgcggc ggatgaactg ggcgaacgcg aggttcaggc
     3721 gcaggcttca cgcttggctg atgtcgtgcg tcagaccctg gcgcggcgcg gcgcgctcga
     3781 cggcgccgag atcaatctga ccctgacgga cctgaagccc aaccgtccga ccatgcagca
     3841 gatgatcgac cggcctggac tggacggcat acgcagcatc tcgaccggcg gcgctacggt
     3901 caccggccac gtgaccacgg ctgacggcca gatccacccg gtcgcctacg actggtattc
     3961 gtccaacatc acggacgtgc gcggcttcag cgcctggcag gatgcggacc gcgtctatcg
     4021 ccgtctggcc gacaacctcg ccgaaggccg ctacaagggg cgctagagcg accgcgctcg
     4081 ctgcacggcg gctgtgaccg ccgcgcccgc cgcccgctcc agatccccgc tggcggtcat
     4141 ggcgtccagg cccgcgcgcg tggtcc
//
LOCUS       DASVVN010000156         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_374448,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000156 DASVVN010000000
VERSION     DASVVN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374448"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1624)
                     /locus_tag="VF686_02395"
     CDS             complement(<1..>1624)
                     /locus_tag="VF686_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8660689.1"
                     /translation="AKIRGAFESWQPDGAEGPEPDLGVVAQRGPETRILVEPGTQSLV
                     QINYVRNPDLDPDTVTERRENLIRNLGLSVLNRRLGEIARGDNPPFLGAFASNSTVAD
                     SLSIGTVGAAFNPGGLNRALETVEQEQRRLVQFGIADPELQREITDTRTSLQNAVTAA
                     ATRTTSALANGLVGAVNGDNVFSSPATNLELFNAAVEGLTAEQVTAAVRPVFEGQGPV
                     VLVTTPVEIEGGEAAVTAALEASRRTAVTARADDAAMEWPYTDFGTPTQPASRTEIAD
                     LGVTRVDFPNGVRLFVKPTDFRDDQILVNVRTGIGELALPTDRVTSMTFAGSVMGAGG
                     TSKLTVDQLTRVLSGKTYSVNTGIGTDTYSLSGATKPEDLQLEMQVLTAYLVDPGLRA
                     APFELARSQHAAQLAQARATPGGVLGRDLAELLGSGDKRDATPTVEEVAAMSIEEIRA
                     GVTEGRARGPIDIVMVGDVTVEDAVATVANTFGALPDRGAQAQPLAGSDVRRFPAPTA
                     EPVQLKHEGPGEQAIGLIGWPTTDAIGDRLEAR"
BASE COUNT          226 a          533 c          599 g          266 t
ORIGIN      
        1 gccgggcctc aaggcggtcg cctatcgcat cggtcgtcgg ccagccgatc aggccgatcg
       61 cctgctcgcc ggggccttcg tgcttcaatt gaaccggttc agccgtgggg gcagggaagc
      121 gccggacgtc cgagccggcc agcggctgcg cctgcgctcc gcggtcgggc agggcgccaa
      181 aggtgttggc gacggtcgcg acggcgtctt cgacggtgac gtcgccgacc atgacgatgt
      241 cgatcggtcc acgggcgcgg ccctcggtca cgccggcgcg aatctcctcg atcgacatgg
      301 cggcgacctc ctcgacggtc ggggtggcgt cgcgcttgtc tcccgaaccc agcaactcgg
      361 ccagatcacg gcccagcacg ccgccgggcg tcgcgcgggc ttgcgcgagc tgcgccgcat
      421 gctggctgcg cgccagttcg aatggcgcag cgcgcaggcc tggatcgacc agataggcgg
      481 tcagcacctg catctcgagc tgcagatcct cgggcttggt ggccccgctc agcgaatagg
      541 tgtccgtgcc gatgccggtg ttgacgctgt aggtcttgcc gctgaggacc cgggtcagtt
      601 ggtcgaccgt cagtttggac gtcccgccgg cgcccatgac cgagccggcg aaggtcatgg
      661 aggtgacccg gtcggtcggc agggccagct cgccgatgcc ggtgcggacg ttgaccagaa
      721 tctggtcgtc gcggaagtcc gtcggcttga cgaacaggcg gacgccgttg gggaagtcca
      781 cgcgggtgac gcccaggtcg gcgatctcgg tgcgcgaggc cggctgggtc ggcgttccga
      841 agtcggtgta gggccactcc atcgcggcgt cgtcggcgcg ggccgtgacg gccgtccggc
      901 gcgatgcttc cagcgccgcg gtgacggcgg cctctccgcc ctcgatctcg accggggtgg
      961 tgaccagcac cacggggcct tgtccttcga agaccggacg aacggcggcc gtgacctgct
     1021 cggccgtcag accttcaacg gcggcgttga acagctccag attggtggcc ggcgacgaga
     1081 agacattgtc gccattgact gcgccgacca gaccgttggc gagggcggac gtggtccggg
     1141 tcgcggcggc ggtcacggcg ttctgcaggc tcgtacgggt gtcggtgatc tcgcgctgaa
     1201 gctccgggtc ggcgatcccg aactggacca gacggcgctg ctcctgctcg accgtctcca
     1261 gcgctcggtt caggccgccc ggattgaagg cggcgccgac ggtgccgatc gacaggctgt
     1321 cggcgacggt gctgttcgat gcaaaggcgc cgaggaaagg cggattgtcg ccccgcgcga
     1381 tctcgccaag ccggcggttc agcaccgaca ggccgaggtt gcggatcaga ttctcgcggc
     1441 gctcggtgac ggtgtcggga tcgagatccg gattgcggac atagttgatc tggaccagcg
     1501 actgggtgcc gggctcgacc aggatgcggg tttccggccc acgttgggcg accacgccca
     1561 gatcaggctc gggaccttcc gcgccatcgg gctgccagct ctcgaaggcg ccccggatct
     1621 tggc
//
LOCUS       DASVVN010000157         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_400397,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000157 DASVVN010000000
VERSION     DASVVN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400397"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1488
                     /locus_tag="VF686_02400"
     CDS             268..1488
                     /locus_tag="VF686_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8660690.1"
                     /translation="MDSLITAAARALAAGDPLGALGRVALRNDAHALALRGVAMAQLG
                     EFGPARTLLRRAARAFGAKSALARARCVVAEAEVALASRDLAWPERTLEAARLTLEAH
                     GDFANAAHARYLEVRRLVLIGRLDQAEARLAVLDPTLFPPAFRTAHELLTAGIALRRL
                     EATRARAALVRAAWAAAEAGIPALVAEVEAAARMLDQPAARLIRAGIGQLVALDDVET
                     ALTAGALVVDSLRHLVKDPHGSVPLARRPVLFALARALGEAWPADVSRERLVVVAFGG
                     READESHRARLRVEIGRLRGLLRSMAGVEATRRGFVLAPRHARDVVVLAWPVEERHAA
                     VLALLADGEAWSSSALATALGAGPRTVQRALEDLRARGRAHPVGRGRARRWTVPPGPG
                     IATVLLLPRPLAGG"
     gene            1498..2049
                     /locus_tag="VF686_02405"
     CDS             1498..2049
                     /locus_tag="VF686_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HEX8660691.1"
                     /translation="MESTPAEILREYGPFEGAGDVHGVTFDGRYVWFASGDHLNAVDP
                     DTGDIPAPGGGGDSGMAWAEGALWVGQYLDRRIHQVEPETGRILRTITSERYVTGVTW
                     VEGELWHATQEDGASEVRRIDDRTGEVLQSLEMPQGRLVSGLESDGAVRLFCGGGADG
                     RISVIARPGGAGSGDVRTGRNRS"
     gene            2115..>2640
                     /locus_tag="VF686_02410"
     CDS             2115..>2640
                     /locus_tag="VF686_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8660692.1"
                     /translation="MTEVMLDVFRSLAAAGFTEVFCVTGRFDAAHVRAAAEAVRRARE
                     EGVLQVWLVLHAPLAGRLSLSGDAGVAVADWPTPAASFPDLHAGDSETSVMLSIAPDR
                     VDGKRLAGLKATDLPPAAVDRWRRGGAEARMVTPDGYPGAPGDASAAKGTRFVVATSD
                     AYAAAIMAARSPAAE"
BASE COUNT          338 a          903 c         1006 g          393 t
ORIGIN      
        1 ggctaatcga ttagtcaatg caatccagtc gcgaacggcg gcgccggcga ccaggcggtc
       61 cgccgcccgg caggcgaaac cacaggtggg gtggcgaacg ggagtctgcg gctttgcggc
      121 cggcttcggg tggcctagtc atgcatcgtc tccggatcgg aagaggccgc atcggaggcg
      181 tccgcgacgc cgccccgtcc ttcaacgagg ctccatgccg gcgcggctcc gaaggcgcgc
      241 gggagcgaca ggtgtgacgg gaaatcgatg gactcgctga tcacagctgc ggcccgggcg
      301 ctcgccgccg gggatccttt gggcgccctg ggccgcgtcg cgctgcgcaa cgatgcgcac
      361 gccctcgccc tgcggggcgt ggcgatggcg caactcgggg agttcggccc ggcccggacg
      421 ctcctgcggc gggcggcgcg cgcgttcggc gcgaagtcgg cgctggcccg ggcccgttgc
      481 gtcgtcgccg aggccgaggt tgcgctggcg tcgcgcgatc tcgcctggcc tgagcggacg
      541 cttgaggccg cacgcctcac gcttgaggcc cacggcgatt tcgccaacgc cgcccacgcc
      601 cggtacctcg aggtgcgccg attggtgctg atcggacgac tggaccaggc cgaagcgcgt
      661 ctggcggttc tggacccgac cctgttcccg cccgctttcc ggacggccca tgaactgctg
      721 acggcgggaa tagccctgcg acgtctggag gcgacgaggg ctcgcgcggc tctcgtccgc
      781 gccgcgtggg ccgcggcgga ggcgggcatt cccgctctcg tcgcggaagt cgaagcggcc
      841 gcccgcatgc tggatcagcc ggcggcgcgc ctcatccggg ccgggatcgg acagctggtc
      901 gcgcttgacg acgtcgagac ggcgctgacg gccggggcgc tggtcgtgga cagtctgcgc
      961 catctggtga aggatccgca cggatccgtc ccgctcgccc ggcgtcccgt gctgttcgcg
     1021 ctcgctcgcg cgttgggtga ggcatggccc gcagatgtgt cgagagaacg cctcgtcgtc
     1081 gtcgctttcg gcggtcggga ggccgacgaa tcccatcggg cgcggctgcg cgtcgagatc
     1141 ggccggctgc gcggcctgct gcgctccatg gcgggcgtcg aggcgacccg gcgcggcttc
     1201 gtgctcgcgc cccgccacgc ccgtgacgtc gtcgtcctgg cgtggcccgt cgaggaacgg
     1261 catgcggcgg ttctggccct gctcgcggac ggcgaggcgt ggtcgagttc ggcgctggcc
     1321 accgctctgg gcgcgggacc gcgcaccgtg cagcgcgcgc tggaagacct gcgggccagg
     1381 ggcagggccc accccgtcgg ccggggccgt gcgcgacggt ggaccgtgcc ccctgggccc
     1441 ggaattgcga cggtcttgtt actcccccgg cccctcgcgg gcggctagac tgcgctcatg
     1501 gaatcgaccc ccgccgagat tctgcgcgag tacgggccct tcgagggcgc cggagatgtc
     1561 catggcgtca ccttcgacgg ccgctacgtc tggttcgctt ccggagacca cctgaatgcg
     1621 gtcgatccgg acaccggcga catcccggcg cccgggggcg gcggcgactc cggcatggcc
     1681 tgggcggagg gcgcgctgtg ggtcgggcag tatctcgatc gcaggattca tcaggttgag
     1741 cctgaaaccg gacgcatcct gcgcaccata acctcggagc ggtatgtgac cggcgtcacc
     1801 tgggtggaag gcgagctatg gcacgccacc caggaggacg gcgccagtga agtgagacgg
     1861 atcgacgatc gcaccggaga ggtcctgcaa agcctggaga tgccgcaggg gcggctggtg
     1921 tcgggcctgg aatccgacgg cgcggtccga ctgttctgcg gcggcggggc cgacggcaga
     1981 atcagcgtca tcgcacgccc gggcggggcc gggtccgggg acgtccggac aggacggaac
     2041 cgatcctgag ccggtccatc cggtcccgcc cggtcgcgcc ggaggtgcgc gccgcgaccg
     2101 gctgaacgga gatcatgacc gaggtcatgc tggacgtgtt ccgcagtctt gccgctgcgg
     2161 ggttcaccga agtcttctgc gtcacgggcc gtttcgacgc agcccatgtc agggcggccg
     2221 ctgaggcggt gcgccgcgct cgcgaagagg gcgtcctgca ggtttggctg gtcctgcatg
     2281 cgccgctcgc gggccgcctg tcgctgtcgg gagacgcggg ggtggcggtc gccgactggc
     2341 cgacgccggc ggcgtcgttt cccgacctcc atgccgggga ctcggaaact tccgtcatgc
     2401 tgtcgatcgc accggatcgg gtcgatggca aacgtctcgc cggcctgaaa gccaccgact
     2461 tgccgccggc tgcggtcgat cgatggcgtc gcgggggcgc cgaggcccgg atggtgacgc
     2521 cggacggcta tccgggagcg ccgggtgacg cgtccgccgc gaaggggacg aggttcgtcg
     2581 tcgcaaccag cgacgcctac gccgccgcca tcatggcggc gcgatcgccc gccgccgaag
//
LOCUS       DASVVN010000158         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_164136,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000158 DASVVN010000000
VERSION     DASVVN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164136"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VF686_02415"
     CDS             <1..1100
                     /locus_tag="VF686_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX8660693.1"
                     /translation="PSGLALRRSALNGLTGALDRTVGPDGGHATRSPEAGLELLLDLL
                     TLDDALSQVSEAAPDAVRGAIQRLTHALRLQTLPDGRLITMQGGGPSTAARVAAARVH
                     DEAHADAASGTVAGVARIRSPLLTIAADIAAPARGAWSAAACAQPMALEIVCGKDRIV
                     TGCGWTPRAADRQGLRLAPGHSTLTLGDGSTGEPLAGWKGELLDHRLVGRPLHLVVDQ
                     RDGEGAVWLEVEHDGWVHDYGLLHQRRLYLDQRLDELRAEERLHPAPGQKDVVRAIAA
                     PYAVRFQLEPGVQASLARDRRSILLRGHSGRGWWFRTDGPDVAIEPSVHIDEGLTRRS
                     LQIVVRGSARTDSETKIRWKLSPAGAGGDPT"
     gene            1172..2068
                     /gene="trxA"
                     /locus_tag="VF686_02420"
     CDS             1172..2068
                     /gene="trxA"
                     /locus_tag="VF686_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8660694.1"
                     /translation="MSLADPTTLPDDLIKEGSDAGFMTDVIEASKTQPVLVDFWATWC
                     GPCRTLTPMIEKAVRAAGGAVKLVKIDVDKNPAYAGQLRVQSIPTVYAFVNGQPVDGF
                     QGAVPDSQLKAFIDKLTGGESANSDIEQLLSLGEESLSLNDLGGAAQAFAQLLTIEPD
                     NQKAIAGMARVYLADGDVDQARQTIAMAPQDSKEPTVQSVRAQLSLASAAPTGAASEL
                     EARVAANPADHQARYDLSLAQASAGDLKGAVDSLLAIVAADREWNDQAARKQLLVVFD
                     AAGGASDIARDGRRRLSSILFA"
     gene            2078..>2580
                     /locus_tag="VF686_02425"
     CDS             2078..>2580
                     /locus_tag="VF686_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8660695.1"
                     /translation="MAQGYVKAADLPQVIPVFPLPGAILLPRGQLPLNIFEPRYLNMI
                     DDAMAGDRIIGMLQPQAGSSHLPGLSPVGCAGRITSFAETSDGRYLITLTGCARFRLA
                     SELPAQTPYRQIRADFAPFELDLTAPPAGEGALDRDILLDALKAYLEARGLDIDWETA
                     ESAPPEAL"
BASE COUNT          368 a         1022 c          836 g          354 t
ORIGIN      
        1 gcccatccgg cttggccctg cgccgctcgg ccctgaacgg cctgaccggg gcgttggacc
       61 gcaccgtcgg ccccgacggc ggtcacgcca cccgcagccc ggaagccggc ctcgaactgc
      121 tgctcgacct gctgaccctc gacgacgccc tgtcgcaggt ttcggaagcc gcgccggacg
      181 ctgttcgcgg cgccatccag cgcctgaccc atgcgctgcg cctccagacc ctgcccgacg
      241 gccggctgat caccatgcag ggcggcggcc cctcgaccgc ggcccgcgtc gccgccgccc
      301 gcgtccatga cgaggcccat gcggacgccg cgagcggaac cgtcgccggt gtcgcccgca
      361 tccgcagccc gctgctgacc atcgccgccg atatcgccgc ccccgcgcgc ggcgcctggt
      421 ccgccgccgc ctgcgcccag ccgatggcgc tggagatcgt ctgcggcaag gaccggatcg
      481 tcaccggctg cggctggacc ccccgcgcgg ccgaccgtca gggcctgcgc ctcgcccccg
      541 gccattcgac cctgaccctc ggcgacggct cgaccggcga gccgctggcc ggctggaaag
      601 gcgagctgct ggaccaccgc ctcgtcggcc gtcccctgca cctcgtcgtc gaccaacgcg
      661 acggcgaggg cgcggtctgg ctggaggtcg aacatgacgg ctgggtccac gactacggcc
      721 tgctgcacca gcgccgcctg tatctggacc agcgcctcga cgaactgcgc gccgaggagc
      781 ggctgcaccc cgccccgggc cagaaggacg tcgtccgcgc catcgccgcc ccctacgccg
      841 tgcgtttcca gctcgagccc ggcgtccagg cctccctggc ccgcgaccgc cgctccatcc
      901 tgctgcgcgg ccactcgggc cggggctggt ggttccgcac cgacgggccc gacgtggcca
      961 tcgagccctc ggtccatatc gacgagggcc tgacccggcg ctcgctgcag atcgtggtgc
     1021 gcggctcggc ccgcaccgat tccgaaacca agatccgctg gaagctgagc cccgccgggg
     1081 cggggggcga tccgacctga ccttgagacg cgcggtcgtc gcgaccatct gtgcgctgcc
     1141 gcgttacgcc taatccgaca caggacagat catgagcctc gccgatccca ccacgcttcc
     1201 ggacgacctg atcaaggaag ggtccgacgc cggcttcatg accgacgtca tcgaggcctc
     1261 gaagacccag ccggtgctgg tcgacttctg ggccacatgg tgcgggccgt gccggaccct
     1321 gacgccgatg atcgaaaagg ccgtccgcgc cgccggcggc gccgtcaaac tggtcaagat
     1381 cgacgtcgac aagaaccccg cctatgcggg ccagctgcgc gtccagtcga tcccgaccgt
     1441 ctacgccttc gtcaacggcc agcccgtcga cggcttccag ggcgccgtgc ccgacagcca
     1501 gctcaaggcc ttcatcgaca agctgacggg gggcgaaagc gccaactccg acatcgagca
     1561 gctgctgtcg ctgggcgagg aatccctgtc tctgaacgat ctcggcggcg cggcccaggc
     1621 cttcgcccag ctgctgacga tcgagcccga caaccagaag gccatcgccg gcatggcccg
     1681 cgtctatctg gccgacggcg acgtcgatca ggcccgccag accatcgcca tggccccgca
     1741 ggattcgaag gaaccgacgg tccagagcgt ccgcgcccag ttgtcgctcg cttccgccgc
     1801 cccgaccggc gccgcctccg agctggaagc cagggtcgcc gccaaccccg ccgaccatca
     1861 ggcccgctac gacctgtcgc tggcccaggc ctcggccggc gatctgaagg gggcggtcga
     1921 cagcctgctg gccatcgtcg ccgccgaccg cgagtggaac gaccaggccg cccgcaaaca
     1981 gctgctggtc gtcttcgacg ccgcgggcgg ggcctccgac atcgcgcggg acgggcgccg
     2041 ccgcctgtcc tccatcctgt tcgcatagga ggcccgggtg gcgcagggat atgtgaaggc
     2101 cgccgacctg ccgcaggtga tccctgtctt cccgctgccg ggcgccatcc tgctgccgcg
     2161 cggccagttg ccgctgaaca tcttcgagcc gcgctatctc aacatgattg atgatgcgat
     2221 ggccggcgac cgcatcatcg gcatgcttca gccccaggcc gggtcgtctc acctgcccgg
     2281 cctgtcgccc gtcggctgcg ccgggcggat caccagcttc gccgagacct cggacggccg
     2341 ctatctgatc accctgaccg gctgcgcccg gttccgtctg gccagcgaac tgccggccca
     2401 gacgccctat cgccagatcc gggccgactt cgccccgttc gagctcgacc tcaccgcccc
     2461 tccggcgggc gagggagcgc tggaccgcga catcctgctg gacgccctga aggcctatct
     2521 ggaggcgcgc ggcctggaca tcgactggga gacggccgag agcgccccgc ccgaagccct
//
LOCUS       DASVVN010000159         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_164219,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000159 DASVVN010000000
VERSION     DASVVN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164219"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VF686_02430"
     CDS             complement(<1..532)
                     /locus_tag="VF686_02430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEX8660696.1"
                     /translation="MTDLPSLRESLDAHGLSAKKSFGQHFLLDLNVTRKIVRLAGPFE
                     GRAVIEVGPGPGGLTRALLESDAGKVVLIEKDPRFLPLLAELDDGSGRLTVVEADALK
                     VREAELVEGPVHLVSNLPYNVGTPLLIKWLTGPWLPHALTLMFQKEVAERIVAAPGDD
                     AYGRLAVISQAVCEARL"
     gene            complement(542..1543)
                     /gene="pdxA"
                     /locus_tag="VF686_02435"
     CDS             complement(542..1543)
                     /gene="pdxA"
                     /locus_tag="VF686_02435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX8660697.1"
                     /translation="MTGPAPLALSMGEPAGVGPEIIAAAWTALKTEGPVFVVIGDAAL
                     LRAQGRPVQTVTAASDAAAVFARAIPVLDVPLPAAVVPGRPEAANAGPVADWIEQAVN
                     LALSGEASGVVTAPIAKAPLYAAGFRFPGHTEFIAELTADAPFAGTRGPVMMLTAKDL
                     RACLVTIHSPLAEVPELVTVERVCRTARVVHEALKRDFGVAAPRLALAALNPHAGEGG
                     AIGLEEIEVLAPAVELLRAEGITITDPLPADTLFHDAARATYDAVLCLYHDQALIPVK
                     TLDFWGGVNTTLGLPVIRTSPDHGTGFDIAGKGVARADSLVAAIRLAGQMAAARAAL"
     gene            complement(1543..>1944)
                     /locus_tag="VF686_02440"
     CDS             complement(1543..>1944)
                     /locus_tag="VF686_02440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8660698.1"
                     /translation="QVMIELPETATDADVAAATARLEALRPQLTCETMLARATSEQGL
                     LGSDLGEADVQNLAPQFQQVARSAEVGSVSTPVRTPLGVHLLGVCGRRAGGVEAPDAR
                     AVEAALFRQNLATLGKRYMRDLREDALIEMK"
BASE COUNT          281 a          689 c          705 g          269 t
ORIGIN      
        1 ccagacgcgc ctcgcacacg gcctgactga tcaccgccag ccggccatag gcgtcgtcgc
       61 ccggcgccgc gacgatccgc tcggccactt ccttctggaa catcagggtc agggcatggg
      121 gaagccacgg ccccgtcagc catttgatca gcagcggcgt gccgacgttg tagggcaggt
      181 ttgacaccag atgcaccggt ccttcgacca gctcggcctc gcgcaccttg agcgcgtccg
      241 cttcgaccac ggtcagccgc ccggacccgt cgtccagttc ggccagaagc ggcaggaagc
      301 gggggtcctt ctcgatcagc accaccttgc cggcgtcgct ctccagcagg gcccgggtca
      361 ggccgcccgg gccgggcccg acctcgatca cggcgcggcc ctcgaacggg cccgccagac
      421 gcacgatctt gcgcgtcaca ttcaggtcca gcaggaaatg ctggccgaag ctcttcttcg
      481 ccgacagccc gtgggcgtcg agggattcgc ggaggctggg gaggtcggtc acacgcgctg
      541 cttacagcgc cgcgcgcgcc gccgccatct gtcccgccag acggatggcc gcgaccaggc
      601 tgtcggcccg ggccacgccc ttgccggcga tgtcgaaacc ggtgccgtgg tcgggcgagg
      661 tgcggatgac cggcaggccg agggtcgtgt tgacgccgcc ccagaagtcc agcgtcttga
      721 ccgggatgag ggcttggtcg tggtacaggc acaggacggc gtcataggtc gcccgcgcgg
      781 cgtcgtggaa cagggtgtcg gcgggcaggg gatcggtgat cgtgatgccc tcggcgcgca
      841 ggagctcgac cgccggggcc agcacctcga tctcttccag cccgatggcg ccgccttcgc
      901 ccgcgtgcgg gttcagcgcg gccagggcca gccggggcgc ggcgacgccg aagtcgcgct
      961 tcagcgcctc atggaccacg cgcgcggtgc ggcagacccg ctcgacggtg accagttccg
     1021 gcacttcggc cagcggcgaa tggatggtga cgaggcaggc gcgcaagtcc ttcgccgtca
     1081 gcatcatcac gggcccccgc gtgccggcga acggcgcgtc ggcggtcagt tcggcgatga
     1141 actcggtgtg tcctggaaag cggaagcccg cggcgtacag gggggctttg gcgatcgggg
     1201 cggtgaccac gcccgacgcc tcaccggaca gggcgaggtt taccgcctgc tcgatccagt
     1261 cggccacggg gcccgcgttg gcggcctcgg gccgtcccgg gacgacggcg gcgggcaggg
     1321 gaacgtccag caccggtata gcgcgcgcga acacggcggc cgcgtcggac gccgccgtca
     1381 cggtctgcac cggccggccc tgcgcgcgca gcagggcggc gtcgccgatg acgacgaaga
     1441 ccggaccttc ggtcttcagg gccgtccagg ccgcggcgat gatctcgggt cccacgcccg
     1501 ccggttcgcc catcgacagg gccagcggcg cggggccggt catcacttca tctcgatgag
     1561 ggcgtcctca cgcaggtcgc gcatataacg cttgcccagg gtggcgaggt tctgtcggaa
     1621 caacgcggct tccacggcgc gggcgtcggg agcctcgacc ccgccggccc ggcgtccgca
     1681 gacgcccaac aagtgaacgc caagcggggt gcggaccgga gtgctgaccg aaccgacctc
     1741 ggccgaacgg gccacctgct ggaactgggg cgccaggttc tgcacgtcgg cctcgcccag
     1801 atcggagccc agcaggccct gttcggaggt cgcgcgcgcc agcatcgtct cgcaggtcag
     1861 ctggggccgc agggcctcca gacgggcggt ggccgcggcc acgtcggcat cggtggcggt
     1921 ttccggcagc tcgatcatca cctg
//
LOCUS       DASVVN010000160         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_320553,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000160 DASVVN010000000
VERSION     DASVVN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320553"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF686_02445"
     CDS             complement(<1..571)
                     /locus_tag="VF686_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660699.1"
                     /translation="MLVDAIRAENFRLSKNRTALFWSLMFVPIISVVIGAISSFVLKG
                     SETKILGDAKMPPELKEALARGTLDMSHALVSAAGNFANPLVLLFVLIGAATIYAGDY
                     RWETWRLISARNSRPNLLLAKLAVVAGVALVAMALMLVSSLIENLIRAAVFDRQLTFT
                     MTAEMAGRFVGFFALSWVRIVQFTMMGMLA"
     gene            complement(561..1463)
                     /locus_tag="VF686_02450"
     CDS             complement(561..1463)
                     /locus_tag="VF686_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8660700.1"
                     /translation="MTTVIETRGLTKTYGTVRALDGLDLSIPRGGVYGVLGPNGAGKS
                     TLFRILLGLIRASDGTATVMGGGVGDVAAMRRMGSMIETPRFPPYMTARQVLRWLALE
                     HGVAAEADVPRWLDRVGLTEAADRKVRGFSVGMLQRLGVAGALLSKPELVILDEPTSG
                     MDPPGIQEMRALIRSLADDDGVTVVLASHQLLEVQRVCDRVAIMNRGKLVREGAVSEL
                     TASGERLRLSVKPVERVAEILGAKGTRDGAAVLAHVTHAEAPALIRALVEAGIEIDEA
                     RWVGADLESVFMTETGHQETPHAG"
     gene            complement(1512..>1715)
                     /locus_tag="VF686_02455"
     CDS             complement(1512..>1715)
                     /locus_tag="VF686_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8660701.1"
                     /translation="RFAYYIDVRDVDALYAELKPKLDTLPPADVHGPADKSYGQRELL
                     VLAPDGELIAFGMAMPQAEVAKA"
BASE COUNT          287 a          604 c          558 g          266 t
ORIGIN      
        1 ccgccagcat ccccatcatc gtgaactgca cgatgcgcac ccacgacagg gcgaagaagc
       61 cgacgaagcg gccagccatt tcggcggtca tggtgaaggt cagttgccga tcgaagaccg
      121 cggcccgaat cagattctcg atcagactgg agaccagcat caacgccatc gcgacaaggg
      181 cgacgccggc gacgaccgcc agcttggcca gcagcagatt gggacggctg ttgcgggcgc
      241 tgatcagccg ccaggtctcc cagcgataat cgccggcgta gatcgtcgcc gcgccgatca
      301 gaacgaacag caacaccagc gggttggcga aattcccggc cgcggacacc agggcgtgac
      361 tcatatccag cgtgccgcgc gccagcgcct ccttcaactc gggcggcatc ttggcgtcgc
      421 ccagaatctt cgtctcgctg cccttgagca cgaaactgct gatcgccccg atgaccaccg
      481 agatgatcgg cacgaacatc aggctccaga acagggccgt cctgttcttc gacaggcgga
      541 agttttccgc gcggatggcg tcaaccagca tggggcgtct cctgatggcc ggtctcggtc
      601 atgaagacgc tctcgaggtc ggcgccgacc cagcgcgcct cgtcgatctc gatcccggcc
      661 tcgaccaggg cccggatcag ggcgggcgcc tcggcatggg tgacatgggc cagcacggcc
      721 gcgccgtccc gcgttccctt ggctcccaga atctcggcga cccgttccac cggcttcacc
      781 gacaggcgca ggcgttcgcc cgacgccgtc agctcgctga ccgcgccctc gcgcaccagc
      841 ttgccgcggt tcatgatggc gacgcggtcg cagacgcgct ggacctccag cagttggtgg
      901 ctggccagga ccacggtcac gccgtcgtcg tcggccaggc tgcggatcag ggcccgcatc
      961 tcctgaatgc cggggggatc catgccgctg gtcggctcat ccagaatgac cagttcgggc
     1021 ttgctcagca gggccccggc aacgcccagc cgctgcagca tgccgaccga gaagccgcgc
     1081 accttgcggt ccgccgcctc ggtcaggccg acgcggtcca gccagcgggg aacatcggct
     1141 tcggccgcca cgccatgctc cagcgccagc cagcgcagca cctgtcgggc ggtcatatag
     1201 ggcgggaagc ggggggtctc gatcatggag cccatgcggc gcatggcggc cacgtcgccc
     1261 acgccgccgc ccatcaccgt cgccgtcccg tccgaggcgc ggatcaggcc cagcaggatg
     1321 cggaagaggg tcgacttgcc ggccccgttc gggcccagca cgccatagac gccgcccctc
     1381 gggatcgaca ggtccagccc gtcgagtgcg cgcacggttc cgtaggtctt ggtgagccca
     1441 cgggtttcga tcacggttgt catgccgtca gcttgacctt gtgtcccccg ttcgcgcaag
     1501 gcgtcgcggg attaagcttt cgcaacctcg gcttgcggca tggccatgcc gaatgcgatc
     1561 agctcgccat cgggggcgag gaccagcaac tcccgctgcc cgtaggactt gtccgcgggc
     1621 ccgtgaacgt cggcgggcgg caaggtgtcg agcttcggct tcagctcggc gtacagcgcg
     1681 tccacgtcgc gtacgtcgat gtaataggcg aagcg
//
LOCUS       DASVVN010000161         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_426725,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000161 DASVVN010000000
VERSION     DASVVN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426725"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF686_02460"
     CDS             <1..547
                     /locus_tag="VF686_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2083 domain-containing protein"
                     /protein_id="HEX8660702.1"
                     /translation="RLQARFGIGFEQAAHRLTTLSRPTARGAPFFLMRVDQAGNISKR
                     FASGAFPFSRFGGACPRWRLHSAFRTPGKIVTQIIETPDGSRWFTLARTVDRQGHDAF
                     TEGHDLAIGLGCELKHAHRLIYARGLDLTAPEVTPIGPACRLCHRHPCAERAAPPVDR
                     PLAVDDWAKSVSPWPFAGPAN"
     gene            complement(711..1694)
                     /locus_tag="VF686_02465"
     CDS             complement(711..1694)
                     /locus_tag="VF686_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8660703.1"
                     /translation="MRHDKAALVIEMARRMAASAEGITLDEISREAGVGRRSAERLRD
                     AVLALFPQAEEVSDPPNKRWRIRGGLSAFEQAPTTEELVELTKAAQGLRATGEPGRAA
                     ALEGLERKLKSAMRSTTLNRLAPDLEALVRAETIAVQAGPRPSADETVLTEIRQAVLA
                     GQPLGFIYSRPGAEARRRSVAPCGVMFGRANYLVAADRESGRIQTFRLDRMSAVKAEA
                     GVATPPADFDLQAFASQSFGIYQDEIEDVVLKVTPEGAAEARAWRWHPTQAVEDQADG
                     GVVVRFRASGMRELAWHLFSWGDQVAIVAPERLKAVMKGELEAARRGLGIN"
     gene            1780..2010
                     /locus_tag="VF686_02470"
     CDS             1780..2010
                     /locus_tag="VF686_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660704.1"
                     /translation="MARAFAPQASVAASRYLIVPCDAEGCGDLAVIWDRLEEQVIDVI
                     DASASARYADEDALWDEARPAFAPAQDFRMAA"
     gene            complement(2011..2433)
                     /locus_tag="VF686_02475"
     CDS             complement(2011..2433)
                     /locus_tag="VF686_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8660705.1"
                     /translation="MAYEAKTKPTEISVADFIAGVENPQRRADAETLCALFEEISGEP
                     PKMWGPSIIGFGTYHYRYASGHEGDAPRLGFSPRKAQTVLYVMSGAEGQAEMIARLGK
                     VKTSVACMYVNRLDQIDLDVLREMAVASLKQMRELYPD"
     gene            complement(2436..>3192)
                     /locus_tag="VF686_02480"
     CDS             complement(2436..>3192)
                     /locus_tag="VF686_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8660706.1"
                     /translation="ADMPVVVSMLNSYSGWAAAAMGFTLHNTAMIITGALVGSSGAIL
                     SYIMCKGMNRSFISVIAGGFGGEVAGAAGPAETRPVKQGSAEDAAFIMKNAGSVIIVP
                     GYGMAVAQAQHTLREMADALKHEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFE
                     LEDINSEFATADVAFVIGANDVTNPSAKTDPQSPIYGMPILDVERAKTVLFIKRGMGS
                     GYAGVENELFFRDNTMMLFADAKKMVEGIVKAL"
BASE COUNT          461 a         1203 c          993 g          535 t
ORIGIN      
        1 gcggcttcag gcccggttcg gcatcggctt cgaacaggcc gcccaccgcc tgaccaccct
       61 gtcccggccc acggcgcggg gcgcgccctt cttcctgatg cgggtcgatc aggccggcaa
      121 tatctccaaa cgcttcgcct cgggcgcctt tcccttctca cgcttcggcg gcgcctgccc
      181 gcgctggcgt ctgcacagcg ccttccgcac gccggggaag atcgtcaccc agatcatcga
      241 gacgccggac ggcagccgct ggttcaccct cgcccgcacc gtcgatcgtc agggccacga
      301 cgccttcacc gaaggccacg acctcgccat cggcctcggc tgcgagctga agcacgccca
      361 ccgcctgatc tatgcccgcg gcctcgatct gacggcgccc gaggtcaccc ccatcggccc
      421 cgcctgccgc ctgtgccacc gccacccctg cgccgagcgc gccgccccgc cggtcgaccg
      481 gccgctggcg gtcgacgact gggccaagtc ggtcagcccc tggccgttcg cgggcccggc
      541 caactgacgg atgaaccaag tccggcttcg cggatttaga cggctcgcct gatcagagag
      601 ccccgtgaat cctgtgtcgt ttgtccgtaa gcacgggctc ctgctgtttg tgatcgtcga
      661 actggtgacc gccgcagcgc tggccggctg gcttctggca gggctgatcc tcagttgatc
      721 cccagcccgc ggcgcgcggc ctccagctcg cccttcatca cggctttcag cctctccggc
      781 gcgacgatcg ccacctgatc gccccagctg aacaggtgcc aggccagttc gcgcatgccc
      841 gaggcccgga accggaccac cacgccgccg tcggcctggt cttccaccgc ctgcgtcgga
      901 tgccagcgcc atgcccgggc ctcggcggcg ccctcgggcg tgaccttcag caccacatcc
      961 tcgatctcgt cctgatagat gccgaacgac tggctggcga aggcctgcag gtcgaagtcc
     1021 gccggcggcg tcgccacgcc cgcctcggcc ttgaccgcgc tcatccggtc gaggcggaag
     1081 gtctggatgc ggccactctc gcggtcggcg gcgaccagat agttggcgcg cccgaacatg
     1141 acgccgcacg gcgcgacgct gcgccgccgc gcctctgccc ccggccggct gtagatgaag
     1201 cccagcggct gccccgccag aacagcctgc cggatctccg tcagcaccgt ctcgtccgca
     1261 gagggccgcg gtccggcttg gaccgcgatg gtctcggccc ggaccagcgc ctccagatcg
     1321 ggggccagcc ggttcagcgt cgtcgagcgc atcgccgatt tcagcttgcg ttccagccct
     1381 tccagcgcgg cggcccggcc cggttcaccc gttgcacgca gcccctgcgc cgccttggtc
     1441 agctcgacca gttcctcggt cgtgggggcc tgttcaaagg ctgacagccc gccgcgaatg
     1501 cgccagcgct tgttcggcgg gtccgacacc tcctccgcct gcggaaacag cgccagcacc
     1561 gcgtcgcgca gccgttcggc ggagcgccgg cccacgccgg cctcgcgcga gatttcgtcc
     1621 agcgtgatcc cctcggcgct ggcggccatc cgccgcgcca tctcgatcac caaagccgcc
     1681 ttgtcgtgtc tcatgaaaag aaaatcgcat acgaccgttt ttgacgcaac gctgcttcat
     1741 ggtcggtttc aacagagacc tgaaccccgg agacatccca tggcccgcgc cttcgccccc
     1801 caagcttccg tcgccgcttc ccgctatctg atcgtgccct gcgacgcaga gggctgcggc
     1861 gatctggccg tcatctggga ccggctggag gaacaggtga tcgacgtgat cgacgccagc
     1921 gccagcgccc gctacgccga cgaagacgcc ctgtgggacg aggcccgccc ggccttcgcc
     1981 ccggcccaag acttccgcat ggcggcctga tcagtcggga tagagctcgc gcatctgctt
     2041 cagcgacgcc acggccattt cgcgcaggac gtccaggtcg atctgatcca gccggttgac
     2101 gtacatgcag gccacgctgg tcttcacctt gcccagacgc gcgatcatct ccgcctgccc
     2161 ctcggcgccg gacatgacgt agagcaccgt ctgggccttg cgcggcgaga atcccagccg
     2221 gggcgcgtcg ccctcatgcc cgctggcgta ccggtagtgg tacgtgccga agccgatgat
     2281 cgacggcccc cacatcttcg gcggctcccc gctgatctcc tcgaacaggg cgcacagggt
     2341 ttcggcgtcc gcgcggcgct gaggattctc gacgcccgcg atgaagtcgg cgacggagat
     2401 ttccgtcggt ttggtcttcg cttcgtaggc cattgtcaga gcgccttcac gatcccctcg
     2461 accatcttct tggcgtcggc gaacagcatc atggtgttgt cgcggaagaa cagctcgttc
     2521 tcgacgcccg cgtagcccga gcccatgccg cgcttgatga acagcacggt cttggcgcgc
     2581 tccacgtcca ggatcggcat gccgtagatc ggcgactgcg ggtcggtctt ggccgacggg
     2641 ttggtcacgt cgttggcccc gatgacgaac gccacgtcgg cggtggcgaa ctcgctgttg
     2701 atgtcctcga gctcgaacac ctcgtcgtag ggcacgttgg cctcggccag cagcacgttc
     2761 atgtgccccg gcatgcggcc cgccaccggg tggatggcgt atttcacctc cacgccctcg
     2821 tgcttcagcg cgtcggccat ctcgcgcagc gtgtgctgcg cctgcgccac cgccatgccg
     2881 tagcccggga cgatgatcac cgagccggcg ttcttcatga tgaaggcggc gtcctcggcc
     2941 gagccctgtt tcaccggccg cgtctctgcc gggcccgcag ccccggcaac ctcgccgccg
     3001 aagccgcccg cgatgaccga gatgaaggag cggttcatcc ccttgcacat gatgtaggag
     3061 aggatcgcgc ccgacgagcc gaccagcgcg ccggtgatga tcatcgcggt gttgtgaagg
     3121 gtgaagccca tcgccgccgc cgcccagccc gaatacgagt tgagcatcga gacgaccacc
     3181 ggcatgtccg cg
//
LOCUS       DASVVN010000162         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_268910,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000162 DASVVN010000000
VERSION     DASVVN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268910"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="VF686_02485"
     CDS             complement(<1..1544)
                     /locus_tag="VF686_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX8660707.1"
                     /translation="MSNYLWLVIAAGALGVLYGLIQTAALMKASTGNDKMREIAAAIQ
                     EGASAYLRRQYTTIGMVGVVILIAAWLLIGPYAAIGFVIGAVLSGLAGFAGMLISVRA
                     NVRTAQAASESLAKGLDLAFRSGAITGMFVAGGALLGVAGYYAFLTAGAGFESTSREV
                     IDGLVALGFGASLISIFARLGGGIFTKGADVGGDMVGKVEAGIPEDDPRNAATIADNV
                     GDNVGDCAGMAADLFETYAVTTVATMVLAAIFFRDQPYVETMMLLPLAICAICVVTSI
                     IGSFFVRLGKKQNIMGALYQGLIVTGVLSVGAVWWVITQLVTGPVTTTSGLVIEPMSL
                     FWSGLVGLGVTAAIVVITEYYTGSNFRPVRSVANASVSGHGTNVIQGLAVSLESTALP
                     ALTIIVGIIASYQLAGLFGIAIATTTMLGVAGMIVALDAFGPVTDNAGGIAEMAGLPS
                     EVRHSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYPSDLEYFAANPDIYPFFAN
                     MGEVAF"
     gene            complement(1720..2145)
                     /locus_tag="VF686_02490"
     CDS             complement(1720..2145)
                     /locus_tag="VF686_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660708.1"
                     /translation="MRRRDLIAASVALPVLAAGREAMASDRPAPGGLSISLPGVGLPI
                     IAGGRLRNYVFVSLRLHLGGSATPESMRPKEAFLRDALVRAGHRTPFTVAGDWTVVDT
                     AALSASLVRSAAAIAGRGSVTRVEVVSQAPRRRTGVNPG"
     gene            complement(2183..>2846)
                     /gene="thiL"
                     /locus_tag="VF686_02495"
     CDS             complement(2183..>2846)
                     /gene="thiL"
                     /locus_tag="VF686_02495"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX8660709.1"
                     /translation="LGIDQKAFGVALLGGDTVSTAGPASFSLTMLGWIPKGRTVGRAG
                     ARPGDLVFVTGAIGDGWLGLEAAKGKLSLEPERLAALSEHYRTPMPRVEFAAVVRDLA
                     SASIDVSDGLIADLGHVAEASRVCIEIDLEPVPLSAAGQAWFDGRVDEQAAMEKLVTG
                     GDDYEIAFTAPARDEAAVRREAERRHLRLTKIGRVTEGQGVVARFGGKAVAFDRPGFT
                     HG"
BASE COUNT          486 a         1017 c          936 g          407 t
ORIGIN      
        1 tcgaaggcga cctcgcccat attggcgaag aacggataga tgtccgggtt ggcggcgaaa
       61 tactccaggt cggacggata ggccgcgaac agcaccaggg cgcccagacc ggcggagccg
      121 atcgcatagc ccttggtcac cgctttcgtc gtgttgccga cggcgtcgag ggcgtcggtc
      181 gagtgacgga cttccgaggg aagacccgcc atctcggcga tgccgccggc gttgtcggtg
      241 accggaccga aggcgtccag cgccacgatc atccccgcca cgcccagcat ggtcgtggtg
      301 gcgatggcga tgccgaacag gccggccagc tggtagctgg cgatgatgcc gacgatgatc
      361 gtcagcgccg gcagggcggt cgattccagc gaaacggcca gaccctggat cacgttcgtg
      421 ccgtggcccg aaaccgaggc gttggcgacc gagcgcaccg gacggaagtt ggagccggtg
      481 tagtattcgg tgatcaccac aatggccgcg gtgacgccca gaccgacgag gccggaccag
      541 aacaggctca tcggctcgat gaccagaccg ctggtcgtcg tgaccgggcc ggtgaccagc
      601 tgggtgatga cccaccagac cgcgccgacc gacagaacgc cggtgacgat caggccctga
      661 tacagggcgc ccatgatgtt ctgcttcttg ccaagacgga cgaagaagct gccgatgatc
      721 gaggtcacta cgcagatggc gcagatggcc agcggcagca gcatcatggt ttcgacatag
      781 ggctggtcgc ggaagaagat cgccgccagc accatcgtcg ccaccgtcgt caccgcatag
      841 gtctcgaaca ggtcggcggc catgccggcg cagtcgccga cgttgtcgcc cacgttgtcg
      901 gcgatggtcg cagcgttgcg ggggtcatcc tcggggatgc ccgcctcgac cttgcccacc
      961 atgtcgccgc ccacgtcggc gcccttggtg aagatgccgc cgcccagacg ggcgaaaatg
     1021 gagatcagcg aggcgccgaa gcccagcgcc accagaccgt cgatgacctc gcggctggtg
     1081 ctctcgaagc ccgcgccggc ggtcaggaag gcgtagtagc cggccacgcc cagaagcgcg
     1141 ccgcccgcca cgaacatgcc ggtgatggcg cccgaccgga aggccaggtc cagacccttg
     1201 gcgaggctct ccgacgccgc ctgcgcagtg cggacgttgg cccggaccga gatcagcatg
     1261 ccggcgaaac cggccaggcc cgaaagcacc gccccaatga cgaagccgat cgccgcgtag
     1321 ggaccgatca gcagccaggc cgcgatcagg atcacgaccc cgaccatgcc gatggtggtg
     1381 tattgccggc gcagataggc cgaggcccct tcctggatcg ccgcggcgat ctcgcgcatc
     1441 ttgtcgttgc cggtgctggc cttcatcagc gcggcggtct ggatcagccc gtagaggact
     1501 cccagcgcgc ctgcggcgat gaccagccac agatagttac tcatggcgtt tccaaaccct
     1561 gtgtgttcag gcgcccggtt ccttggcgcg gcgggcgtta ttcgcctctg tccgccttcc
     1621 ggtccccccg tgcgcacgac gcatgttgcg tccttgggga attgcggcgg aaacgtgcca
     1681 aatgccggag cccgaggcaa caggcttggt gaacgaagtt cagcccgggt tcacgcccgt
     1741 acgccgccgg ggcgcctggc tgacgacctc gacccgcgtg accgagccgc gtccggcgat
     1801 ggcggcggcc gaacgcacca gactggccga cagggcggcg gtgtcgacca cggtccagtc
     1861 gcccgccacc gtgaacggcg tccgatgccc cgcccgcacc aaggcgtcgc gcaggaaggc
     1921 ctctttcggc cgcattgatt cgggggtcgc cgacccgccc agatgcagtc tcagcgacac
     1981 gaagacataa ttcctcagcc gcccgcccgc gatgatcggc aggcccacgc ccggcaagct
     2041 gatgctgagc ccgccgggcg cgggccggtc gctggccatg gcttcgcgac cggcggcgag
     2101 aaccggcagg gcgacggagg cggcgatgag gtcacgacgg cgcatggcgg caccttgccc
     2161 gccacgcctt gagatttcgt tgtcagccgt gggtgaagcc cggcctgtcg aatgctacgg
     2221 ccttgccgcc gaaacgggcc accactccct gcccctccgt cacccgcccg atcttcgtca
     2281 ggcgcagatg ccgccgctcg gcctcgcggc gtaccgcggc ctcgtctcgc gcgggcgcgg
     2341 tgaaggcgat ctcgtagtcg tcgccgcccg tgaccagctt ctccatcgcc gcctgctcat
     2401 cgacgcgccc gtcgaaccag gcctgacccg ccgccgacaa cggcacgggt tccagatcaa
     2461 tctcgatgca gacccgactg gcctcggcca catggcccag atccgcgatc agcccgtccg
     2521 acacgtcgat ggaggcgctg gccagatccc gcaccacagc cgcgaactcc acgcgcggca
     2581 tcggcgtccg atagtgttcc gacagggcgg ccagccgctc aggctccagc gacagcttgc
     2641 cctttgccgc ctccagcccc aaccagccgt cgccgatcgc gcccgtcacg aacaccagat
     2701 cgccgggccg cgcgcccgcg cgtccgacgg tccgcccctt gggaatccag cccagcatgg
     2761 tcagggagaa gctggccggc cccgcggtcg aaaccgtatc cccgcccagc aaggccaccc
     2821 cgaacgcctt ctggtcgatc cccagg
//
LOCUS       DASVVN010000163         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_426754,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000163 DASVVN010000000
VERSION     DASVVN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426754"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /gene="flgK"
                     /locus_tag="VF686_02500"
     CDS             complement(<1..1433)
                     /gene="flgK"
                     /locus_tag="VF686_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX8660710.1"
                     /translation="MSLNAIMNTATSGLATAQTQLRVVSDNVSNVNTPGYVRKIADQA
                     TLTSQGMGSGVEVSRIRLATDRFLQAASLNAGANAAREGVRYELYDRIQSLFGDPGGD
                     TGFFSQVDSVFSAFAASAEDPTSSPRRQEALFKTQALFDEASRIGKQIQAVREDADGR
                     VQSAVEKTNGLLKQIESLNLEIARATAVNGDASGAETAQAALIGQLAELVDIRVSTRA
                     VGGVSIRTGAGTLLAGEGAATLEYNRAGTVTGETAFNEIWITEPGGQKRSLMDSVVSG
                     EIKGLVELRDVEAPQAAERLAELVSHVADELNRAHNANSSVPAPVRLTGRNVGQTFET
                     AIAGFTGRTTVAVTNAAGVIQSRADIVFSGATMTVNGAPTTPANFLAELNLQLGGAAT
                     ASFTDGRLSITATAPNGVAVADDPTTPSGKAGRGFSHYFGLNDLISTDRPAFYDNGLT
                     VGSTLGFTAGEKLTFRFTGDTGSRLRDL"
     gene            1827..>2336
                     /locus_tag="VF686_02505"
     CDS             1827..>2336
                     /locus_tag="VF686_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660711.1"
                     /translation="MSALALPSVTAPAKASVPQGSTGAADATGFKSLLATCLDKESAP
                     VEAEGPEAAPANDDTEAAATASLLMTPIPAPPPSVAAPVAPEAEATAPAVEAPAEFDP
                     STLLPDSDGQSAAVAPTTGKSPEPTPAAAPATAPVMAVAPTPVGTDAPARDAPAPAPA
                     PAPAPAKAEA"
BASE COUNT          335 a          821 c          804 g          376 t
ORIGIN      
        1 agatcgcgca gccgcgagcc ggtgtcgccc gtgaaccgga acgtgagttt ctcgccggcg
       61 gtgaagccga gggtggagcc cacggtcagc ccgttgtcgt agaaggcggg gcggtcggtc
      121 gagatcaggt cgttcaggcc gaaataatgt gagaagccgc ggccggcctt gccggacggc
      181 gtggtcgggt cgtccgcgac ggcgacgccg ttcggtgcgg tcgcagtgat gctgagacgg
      241 ccgtctgtga agctggctgt ggcggccccg cccagctgga ggttcagctc ggccaggaag
      301 ttggccggcg tggtgggcgc gccgttgacc gtcatggtcg cgcccgaaaa gacgatgtcc
      361 gcccgactct ggatgacgcc cgccgcattg gttaccgcga ccgtagtccg gccggtgaag
      421 ccggcgatgg ccgtctcgaa cgtctggccc acattgcggc cggtcagacg caccggggcg
      481 ggaaccgagg agttggcgtt gtgggcccgg ttcagctcgt cggcgacatg ggacaccagt
      541 tccgccagcc gttcggcggc ctgcggagcc tcgacgtcac gtagttcgac cagacccttg
      601 atctcgccgg agacgacgct gtccatcaat gagcgcttct ggccgcccgg ttcggtgatc
      661 cagatttcgt tgaaagccgt ttcgcctgtg accgtgccag ccctgttgta ctccagggtc
      721 gccgcacctt cgccagccag cagggtgccc gctcccgtgc ggatcgacac gccgccgacg
      781 gcccgggtcg aaacccggat atcgaccagt tcggccagct gtccgatcag ggctgcctgg
      841 gccgtttccg cgccagaggc gtcgccgttc accgccgtcg cgcgggcgat ctccaggttc
      901 aggctctcga tctgcttgag caggccgttg gtcttttcca ccgcgctctg gacgcgaccg
      961 tcggcgtcct cgcggaccgc ctggatctgt ttgccgatgc ggctcgcctc atcgaacagg
     1021 gcctgggtct tgaacagggc ctcctgacgg cgcggcgagg aggtgggatc ctcggcgctg
     1081 gcggcgaagg ccgagaacac gctgtcgacc tgggagaaga agccggtgtc gccgcccgga
     1141 tcgccgaaca gcgactggat gcggtcatac agctcatagc ggacgccttc ccgcgcggcg
     1201 ttggcgcctg cgttcagact ggcggcctgg aggaaacggt cggtcgccag gcggatgcgc
     1261 gaaacctcga cgccggatcc catgccctgg ctggtcaggg tggcctgatc cgcgatcttg
     1321 cggacatagc cgggcgtgtt gacgttggag acgttgtccg agacgacgcg cagctgggtc
     1381 tgggccgtgg ccagccccga ggtcgccgtg ttcatgatgg cgttcagcga catccaacgg
     1441 cctccatacg ggcggcgccc ggcaagccct gccagccgac gagcgttttt tgcccggcgt
     1501 cccgcgaggg ttcgggcggc aagtctttga ttttacgcgc tatggcgttt cgcgcctggg
     1561 tcatgaccgt tcaggagcgc aaggtgcggg ccatccacgc ccggctgctc ccatgtctct
     1621 ggcgccgggc aagaatcgtc ggcacgcggc aagtttggac cggacgccgg gcgtcgacgc
     1681 gtttcaccag gccagacttt tcgacgtctt ttcaatcgct tccggccacc gccgcggttg
     1741 gcccgcgact tgcgccatgg gttccgaatg tccggcgcag cacgcgcccc agccaccatc
     1801 gccgttgcga ccatcccatc gggacaatgt ccgccctcgc cctgccgtcc gtcaccgcgc
     1861 ccgccaaagc cagcgtcccg caggggtcga ccggcgccgc cgacgcgacc ggcttcaaga
     1921 gcctgctggc gacctgtctg gacaaggagt cggcgccggt ggaagcggaa gggcccgagg
     1981 ccgcgcccgc caacgatgat acggaggcgg ccgctacggc gagcctcctg atgacgccga
     2041 tcccggcccc tccgccctcc gttgcagcac ccgtcgcacc cgaagccgag gcgaccgcgc
     2101 cggccgtcga ggctccggct gaattcgatc cgtcgacctt gctgccggac agcgacggtc
     2161 agtcggccgc cgtcgcaccg acgacgggga agagcccgga accgaccccg gccgccgccc
     2221 ccgcaacggc tccggtcatg gctgtcgcgc cgacccccgt ggggactgac gcaccggcgc
     2281 gcgacgcccc cgcccccgcc cccgcccccg cccccgcccc cgcaaaggct gaggct
//
LOCUS       DASVVN010000164         4541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_295083,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000164 DASVVN010000000
VERSION     DASVVN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295083"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VF686_02510"
     CDS             complement(<1..1059)
                     /locus_tag="VF686_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8660712.1"
                     /translation="MRTVHGPVIGSRDHGILSFKGVRYGADTRPARFQPPRLPAPWSR
                     PAETFDYGAASPQSGGEANQSEDCLFLNVWTPGLDAGRRPVMVYIHGGAYSTGSGSSP
                     LYDGSRLAARGDVVVVTLNHRLNLFGYAYLARLDAGFPHSGNVGQLDLIQALHWVRDN
                     IKAFGGDPDRVMVFGQSGGGAKIATLMAMPSADGLFHRAATMSGQQVTASGPLNATRR
                     TRAWLDAAGVPPDRVRDLLDMPAEALLEAARATDPVLGYGSLYFGPVVDHQTLFRHPF
                     YPEAPPQSARVPMIIGNTRDETLAFLGGNPANAGLTWETLPGRMAPDVMRIDVAPEIV
                     IAEYRRLYPDWSPDQVLIG"
     gene            complement(1146..2252)
                     /locus_tag="VF686_02515"
     CDS             complement(1146..2252)
                     /locus_tag="VF686_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX8660713.1"
                     /translation="MSALPNLRPSEQCRWDMVALGEVMLRFDPGERRVRNARSFDVWE
                     GGGEYNVVRATAKSFGLRGAVVTALPDNDLGRLADDLIGQGGVDRSHILWREADGMGR
                     NTRLGLNFTERGFGVRPARGLSDRANSAASQMRPGEIDWDRLFGDEGVRWFHTGGIFA
                     ALSSSTAEVAIEAVEAARQHGTVVSYDLNYRASLWDSHPDPDAARRVNRAIAGAVDVM
                     IGDGYGFQTCLGIDLKTPKADSEAGFGSSAFASAAEAFGALYPQAKVVAATLRRPSTA
                     SRNDWSAAAWSGGELHTAPVWTDLEILDRVGGGDSFVSGLAWALMEGLSVSEAVGWGA
                     AHGALAMSTPGDASMVSRQEIAALLAGGDGRSIR"
     gene            complement(2357..>4541)
                     /locus_tag="VF686_02520"
     CDS             complement(2357..>4541)
                     /locus_tag="VF686_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8660714.1"
                     /translation="GFAPQNPGNSGANGTTAANCATGVARPANTAANNAAYTTANQAS
                     VFIPRLPRYGRLTHEQERLGVTGSFQWRPAETTLFTLDVLYSKLDATRQEDFLEALSF
                     SRNAAAGGQTQIHVRDAAVEDNMLVYGVFDNVDIRSESRFDVLTTAYTQINFQAEHDF
                     SDRLRGSFYYGNATSNFENPIQTTVTLDRQNTQGYSYDFRGNPEAPLINYGFDVTNPA
                     NWAWRGANLGNGLAVLPRSEIRLRPNGVDTRFTTAQADIEFDATEFVTFKAGVNFKTY
                     DSDSHESRRADETLVPQLPAGVTVADISNLLTGFGQDLVPTGTDTSWLRPDLDAIADL
                     FNIYCNCDTGVPGGDFRLASITNGNARGGNRSIYEQDLAFYIQADFDTQVFDMPLRGN
                     IGVRQVNTILEAEGYSSTGGGTLVTGENEYSDTLPSLNLVLEPVEDLFLRFGAAKVMS
                     RPPINNNLAGTNFLVPTTSLNTGPNFNATIGNVKLEPFRAATYDFSIEWYFAPESLLS
                     FGYFYKDIDTYIQLVRQDLPYTDLTALNPSAFAPAFCTGACTPSTIFQLTAAVNTEGG
                     PLKGFEISYQQPFRFLPGLLANTGVQLNYTHVESEIDYCNDALCATFVTADLVNLSPQ
                     SYNATLYYEDSKLSARVSAAIRDAYLQNVPGRNGNNIEGKKETFNLDASASYQFTDEI
                     QLTLEALNLTDEENHQFVGDGADRESTSVYHHTGRQVFVGARYRF"
BASE COUNT          737 a         1434 c         1632 g          738 t
ORIGIN      
        1 cccgatcagg acctgatcgg gcgaccagtc gggatacagg cggcggtatt cggcgatgac
       61 gatttcgggc gcgacatcga tgcgcatgac atcgggcgcc atccgcccgg gcagggtctc
      121 ccaggtcaat ccggcattgg cgggattgcc gcccaggaag gccagggtct cgtcacgggt
      181 attgccgatg atcatcggca cgcgggccga ctggggcggg gcttccggat agaaggggtg
      241 gcggaacagg gtctggtggt ccaccaccgg gccgaaatac aggctgccgt agccgagcac
      301 cgggtccgtg gcccgcgccg cctcaagcag ggcttcggcc ggcatgtcga gcaggtcgcg
      361 cacccggtcc ggcgggacgc cggccgcgtc cagccaggcg cgcgtccgcc gcgtggcgtt
      421 cagcggcccg gacgcggtca cctgctggcc gctcatggtc gcggcgcgat ggaacaggcc
      481 gtcagcggaa ggcatggcca tcagggtggc gatcttggcg ccgccgcccg actggccgaa
      541 caccatgacc cggtcgggat cgccgccgaa ggctttaata ttatccctga cccaatgcag
      601 cgcctggatc agatccagct gacccacatt gccggaatgg gggaagcccg cgtccagccg
      661 cgccagatag gcgtagccga acaggttgag ccggtggttc agcgtgacca cgaccacgtc
      721 gccgcgcgcc gccagtctcg atccgtcgta caggggcgac gagcccgacc ctgtggaata
      781 ggcgccgccg tggatataga ccataaccgg ccgccgccct gcgtcgaggc ccggcgtcca
      841 gacgttgagg aacaggcagt cctcggactg gttggcctct ccccccgact ggggcgaggc
      901 ggcgccgtaa tcgaacgttt cggccggccg cgaccagggc gcggggagcc ggggcggctg
      961 gaaccgggca ggccgggtgt cggcgccgta gcggacgccc ttgaacgaca ggatgccgtg
     1021 atcgcgcgag cctatgaccg ggccatggac cgtccgcacc cggggcgacg cggcgccggc
     1081 actcgcggat ggggcagaaa cggccgtcgc cgccagtgcc ccgagggtcg cgcgacgggt
     1141 cagcttcacc ggatcgagcg gccgtcgccg ccggccagca gggccgcgat ctcctgacgc
     1201 gacaccatcg aggcgtcgcc gggcgtgctc atggcgaggg cgccatgggc agcgccccag
     1261 cccacggctt cggagaccga cagcccctcc atcaacgccc aggccagacc ggagacgaag
     1321 ctgtccccgc cccccacccg gtccaggatt tccagatccg tccagaccgg cgctgtatgc
     1381 agttcgccgc cgctccacgc cgccgccgac cagtcgttgc ggctggccgt cgaaggccgg
     1441 cgcagggtgg cggcgaccac cttcgcctga ggatagaggg cgccgaacgc ctcggcggcc
     1501 gaggcgaacg cagacgaacc gaacccggcc tcgctgtcgg ccttcggcgt cttcagatcg
     1561 atgccgaggc aggtttggaa cccgtagccg tcgccgatca tcacatcgac ggcgccggcg
     1621 atggcgcggt tcacgcggcg cgcggcgtcc gggtccgggt ggctgtccca gaggctggcg
     1681 cgatagttga gatcgtacga gaccacggtt ccgtgctgcc gggcggcctc gaccgcctcg
     1741 atggcgacct cggccgtcga cgaagacagg gccgcgaaga tgccgccggt gtggaaccag
     1801 cgtacgccct cgtcaccgaa cagccggtcc cagtcgattt cccccggacg catctggctg
     1861 gccgccgaat tggctcggtc ggacaggccg cgggcgggcc ggacgccgaa gccacgctcg
     1921 gtaaagttca ggcccagccg cgtgttgcgg cccatgccgt cggcctcgcg ccacaggatg
     1981 tggctgcgat ccaccccgcc ttggccgatc agatcatcgg ccaagcggcc cagatcgtta
     2041 tccggcaagg cggtaacgac cgccccgcgc aggccgaacg acttcgccgt ggcccggacg
     2101 acgttgtatt caccgccacc ctcccagacg tcgaaggacc gcgcgttgcg gacccgtcgt
     2161 tcaccgggat cgaaccgcag catgacctcg ccgagggcga ccatgtccca gcggcattgt
     2221 tcggacggac ggagattcgg cagggcggac atcccgaaat cctatacgcc gcgtcctgac
     2281 ggcggtagcg gccaaagaaa gaagggccgg cgactttcgc cgccggcccg aagtgacgtt
     2341 caggagaacg cgtctcttag aagcgataac gggcgccgac gaacacctgg cggcccgtgt
     2401 ggtggtagac ggaggtgctt tcccgatcag cgccgtcgcc gacgaactgg tggttttcct
     2461 cgtccgtcag gttcagggcc tccagcgtca gctgaatctc gtcggtgaac tggtacgagg
     2521 ccgaggcatc gaggttgaag gtttccttct tgccttcgat gttgttgccg ttccggcccg
     2581 ggacgttctg gagataggcg tcccggatcg ccgccgagac ccgcgcggac agcttgctgt
     2641 cctcgtaata gagggtggcg ttgtacgact ggggcgacag gttcaccaga tcggcggtca
     2701 cgaacgtcgc gcagagcgcg tcgttgcaat agtcgatctc ggactcgacg tgggtgtagt
     2761 tcagctgcac cccggtattg gccaggaggc cgggcaggaa gcggaagggc tgctgatagc
     2821 tgatctcgaa gcccttcagc gggccgccct cggtgttaac cgcggccgtc agctggaaga
     2881 tggtcgaggg ggtgcaggcc ccggtgcaga aggccggagc gaaggccgag gggttcaggg
     2941 ccgtcaggtc cgtatagggc aggtcctgac ggacgagctg aatgtaggtg tcgatgtcct
     3001 tgtagaagta gccgaaggac agcaggcttt ccggcgcgaa ataccactcg atgctgaagt
     3061 cgtaggtggc ggcccggaac ggctccagct tcacattgcc gatcgtggcg ttgaagttcg
     3121 ggccggtgtt cagcgaagtg gtcggcacga ggaagttggt gcctgccagg ttgttgttga
     3181 tcggcggacg cgacatcacc ttggcggcgc cgaagcgcag gaacaggtct tcgaccggct
     3241 ccagcacgag gttcagcgac ggcagggtgt cggaatactc gttctcgccg gtcaccaggg
     3301 tgccgccgcc ggtcgagctg tagccctcgg cctccaggat cgtattgacc tggcgcacgc
     3361 cgatgttgcc gcgcaacggc atatcgaaca cttgggtgtc gaagtcggcc tggatgtaga
     3421 aggccagatc ctgctcgtag atcgaccggt tgccgccgcg ggcgttgccg ttggtgatgc
     3481 tggccaggcg gaagtcgccg cccgggaccc cggtgtcaca gttgcagtag atgttgaaca
     3541 ggtcggcgat ggcgtccagg tcgggacgca gccagctcgt atcggttccg gtcgggacca
     3601 gatcctggcc gaagcccgtc agcaggttag agatgtcggc cacggtgacg ccggccggca
     3661 gctgcggcac cagggtttcg tcggcgcgac gcgattcatg ggaatcggag tcgtaggtct
     3721 tgaagttgac gccggccttg aacgtgacga actcggtggc gtcgaattcg atgtcggcct
     3781 gggcagtggt aaagcgcgtg tccacgccgt tgggccgcag gcggatctcc gaacgcggca
     3841 ggacggccag tccgttgccc aggttggccc cgcgccaggc ccagttggcc gggttggtga
     3901 cgtcgaagcc gtagttgatc agcggcgctt ccgggttgcc gcggaaatcg tacgaatagc
     3961 cctgggtgtt ctggcgatcg agggtgacgg tcgtctggat cgggttctcg aagttcgacg
     4021 tggcgttgcc gtagtagaag ctgccgcgca gtcgatccga gaagtcgtgc tcggcctgga
     4081 agttgatctg ggtataggcg gtggtgagca cgtcgaagcg gctctccgag cggatgtcga
     4141 cgttgtcgaa cacgccgtag accagcatgt tgtcctcgac cgccgcgtca cgcacgtgga
     4201 tctgggtctg gccgccggcc gccgcgttgc ggctgaagga cagggcttcc aggaagtctt
     4261 cctgacgggt ggcgtccagc ttggaataca ggacgtccag cgtgaacagg gtggtctcgg
     4321 ccggccgcca ctggaaggag ccggtgacgc ccaggcgttc ctgctcgtgt gtcaggcggc
     4381 cgtagcgcgg caggcgcgga atgaagacgc tggcctggtt cgccgtagtg taggcggcgt
     4441 tgttcgcggc cgtgttggcc ggacgcgcca cgccggtggc gcagttggcc gccgtcgtgc
     4501 cgttggcgcc gctgttgcca gggttctggg gcgcgaaacc g
//
LOCUS       DASVVN010000165         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_31282, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000165 DASVVN010000000
VERSION     DASVVN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31282"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VF686_02525"
     CDS             complement(<1..367)
                     /locus_tag="VF686_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8660715.1"
                     /translation="MAFTLTSTGFADGGALPDAQVYAKGNRSPQLSWSGAPEGTKSFA
                     VTCYDPDAPTGSGFWHWTVANIPADVTELTEGGPVPAGAVEGRTDFGEPGFGGAAPPQ
                     GHGPHRYIFTVFAVDVDSLP"
     gene            complement(424..555)
                     /locus_tag="VF686_02530"
     CDS             complement(424..555)
                     /locus_tag="VF686_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660716.1"
                     /translation="MKRLIQFSVAAFVVVCVLIFAAALAGGLTDDSAFTPAAKEAAH"
     gene            complement(552..788)
                     /gene="purS"
                     /locus_tag="VF686_02535"
     CDS             complement(552..788)
                     /gene="purS"
                     /locus_tag="VF686_02535"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX8660717.1"
                     /translation="MAKVKVHVFLKPGVLDVQGKAVEGALHGLGWAGVANARVGKLIE
                     FDLDGAADPAAEAKKMCEQLLANTVIESYRIEAA"
     gene            complement(790..1548)
                     /gene="purC"
                     /locus_tag="VF686_02540"
     CDS             complement(790..1548)
                     /gene="purC"
                     /locus_tag="VF686_02540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX8660718.1"
                     /translation="MNVKRKKLYEGKAKILYEGPEPGTLIQYFKDDATAFNGEKKAQL
                     EGKGVINNRISEFVMSRLNGIGVTNHFIKRLNLREQLIREVEIIPLEVVCRNVVAGSM
                     SQRLGIDEGTPLPRSIIEFYLKKDELNDPMVTEEHITAFNWATTQELDDILAMTVRVN
                     DYLSGMFGAVGITLVDFKIEFGRIWENDFSRVILADEISPDSCRLWDTNTGEKLDKDR
                     FRRDLGNVIESYTEVARRLGIMKDMPTVIQGGLH"
     gene            1818..2141
                     /locus_tag="VF686_02545"
     CDS             1818..2141
                     /locus_tag="VF686_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX8660719.1"
                     /translation="MTTFDDREKGFESKYALDQEQEFKAVARRNKLLGHWAAEKMGLN
                     AAGAEEYGRAIVKADFEQPGEEDVFRKIAQDLEGAGLTVSEGEIRRKMDELSSVAREQ
                     IRNGD"
BASE COUNT          376 a          723 c          754 g          391 t
ORIGIN      
        1 ccggcaggct gtccacgtcc acggcgaaga ccgtgaagat atagcggtgc ggcccgtgcc
       61 cttggggcgg cgcggcgccg ccgaagccgg gctcgccgaa gtccgtgcgc ccctcgacgg
      121 cgccggccgg cacagggccg ccttcggtca gttcggttac atcggcgggg atattggcca
      181 cggtccagtg ccagaagccc gagccggtcg gcgcatcggg gtcgtagcag gtgacggcga
      241 agctcttcgt gccttccggc gcgccggacc aactaagctg cggcgagcgg ttgcccttgg
      301 cgtagacctg ggcgtccggc agggcgccgc cgtcggcgaa gccggtggaa gtgagggtga
      361 aggccatgga tcgctcctcg ctgcagctgg agccagccta acgcacaatc gcggctgcgg
      421 tcgctagtgc gccgcttcct tggccgctgg cgtgaaggcc gaatcgtccg tgagaccgcc
      481 agccaaggcc gcggcgaaga tcagcacgca gaccacgacg aaggccgcga cagagaactg
      541 gatcagccgc ttcacgcggc ctcgatgcgg tagctttcga tcacggtgtt ggccagcagc
      601 tgctcgcaca tcttcttcgc ctcggcggcc gggtcggctg cgccatccag atcgaactcg
      661 atcagcttgc cgacacgggc gttggcgacg ccggcccagc ccagaccgtg cagggcgcct
      721 tcgacagcct tgccctggac gtccagaacg cccggcttga gaaacacgtg caccttaacc
      781 ttggccattt tagtgcagtc caccctgaat cacggtcggc atgtctttca tgatgccgag
      841 gcggcgggcc acctcggtgt agctttcgat cacattgccc agatcgcggc ggaagcggtc
      901 cttgtccagc ttctcgcccg tgttggtgtc ccacaggcgg caggaatcgg ggctgatctc
      961 gtcggccagg atgacccggc tgaaatcgtt ctcccagatg cggccgaact cgatcttgaa
     1021 gtccaccaag gtgatgccga ccgcgccgaa catgcccgac agatagtcgt tcacccgcac
     1081 cgtcatggcc aggatgtcgt ccagctcctg ggtggtggcc cagttgaagg cggtgatgtg
     1141 ctcttccgtg accatcgggt cgttgagctc gtccttcttg agatagaatt cgatgatcga
     1201 gcggggcagg ggcgtgccct cgtcgatgcc gaggcgctgg ctcatcgagc ccgcgacgac
     1261 gttgcggcag acgacctcca gcggaatgat ctcgacttcg cggatcagct gctcgcgcag
     1321 gttcaggcgc ttgatgaagt ggttggtcac gccgatgccg ttcaggcgcg acatgacgaa
     1381 ttcgctgatg cggttgttga tgacgccctt gccctcgagc tgggccttct tctcgccgtt
     1441 gaaggcggtg gcgtcgtcct tgaaatactg gatcagggtg ccgggctcgg gcccctcgta
     1501 gaggatcttg gccttgccct cgtagagctt cttgcgcttg acgttcatca tgatcggtcc
     1561 gggcggggcg cgtcggaaac gcggatcgcg cggccagacc gtgtctgggc ggcttgatcc
     1621 ggaatcgggc gggcgggaaa aagtcgcgcg aacccttagc ggatgcggcg ccgggacaca
     1681 aaccagcgtc gttttggcac gctccggcgt cgcctcgtcg caaccccgtc agttccggtt
     1741 gcgttcgggt atgcggtaga tatagaccac ctcgaacgcg gccccgctgg gtcgcctgac
     1801 ggagcctcgc gacacccatg accaccttcg acgatcggga aaagggcttc gagtccaagt
     1861 acgccctcga tcaggaacag gagttcaagg cggtcgcccg gcgcaacaag ctcctgggcc
     1921 actgggctgc cgagaagatg ggcctgaacg ccgcgggcgc cgaggaatac ggccgcgcca
     1981 tcgtcaaggc ggacttcgaa cagccgggcg aggaagacgt cttccgcaag atcgcccagg
     2041 acctcgaggg cgccggcctg accgtctcgg aaggcgagat ccgccgcaag atggacgagc
     2101 tgtcgtccgt cgcccgcgaa cagattcgca acggcgacta gccgcctctc gcggcctctc
     2161 acgaagaaag gccgcggcct ttcggctgcg acctctctga acggaccgat tggggacgat
     2221 ccgccgccgg cgccggcgtg accg
//
LOCUS       DASVVN010000166         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_216315,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000166 DASVVN010000000
VERSION     DASVVN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216315"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..486
                     /gene="nrdR"
                     /locus_tag="VF686_02550"
     CDS             28..486
                     /gene="nrdR"
                     /locus_tag="VF686_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8660720.1"
                     /translation="MKCPFCGNQDTQVKDSRPSDDGAAIRRRRSCPQCSGRFTTFERV
                     QLRELMILKRNGRRTPFDRDKLERSLNVALRKRPVQAEQVEQLVSRITRQLESLGETE
                     IPSSTVGDFIMKALKSVDEVAYVRYASVYKDFRHTGDFARFLGDEGLDES"
     gene            486..1106
                     /locus_tag="VF686_02555"
     CDS             486..1106
                     /locus_tag="VF686_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX8660721.1"
                     /translation="MRVTLKLATSLDGRIATASGESQWITGEAARLEGHRLRAAHDAI
                     LVGVETVLADDPELTARLPGRPVDQPLRVVLDSRLRTPPTAKVASGDSLILTVAEPAP
                     IGTAVVERVEADHGRPSVAACLSALKRRGVSSVLIEGGGQVAASFLRAGVVDRLEWFR
                     APILLGGEGRPCVASLALARLSDAPVFRRLAVEPCGDDLWERYERI"
     gene            1108..1698
                     /locus_tag="VF686_02560"
     CDS             1108..1698
                     /locus_tag="VF686_02560"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8660722.1"
                     /translation="MFTGIVTDIGRVRAVRQTERDRRYEIETAWDTAGIDLGASVSHA
                     GCCLTVTEKGPGWFAVEVSGETLSKTSLGDWAEGHRVNLERAAKLGDEMGGHVVSGHI
                     DGLGRVAAVVPEGGSHRVHIEVPAPLHRFIAPKGSITVDGVSLTVNGVEGQRFDVNLI
                     PHTWDATTLGGLKVGDAVNLEIDMLARYLARWQETA"
     gene            1698..>2129
                     /locus_tag="VF686_02565"
     CDS             1698..>2129
                     /locus_tag="VF686_02565"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX8660723.1"
                     /translation="MQLAANMNDSPISPIEDILEDARNGKPYILVDAEDRENEGDIII
                     PAQFATPDQINFMARHARGLICLAITGERARQLRLPPMAAENRTSMGTAFTVSIEARE
                     GVTTGISAADRARTVQVASDPTKGADDIVSPGHVFPLVARDG"
BASE COUNT          339 a          692 c          761 g          337 t
ORIGIN      
        1 gcttcccgat ctacgggtaa cccgctgatg aaatgcccct tctgcggaaa tcaggacacc
       61 caggtgaagg acagccgccc gtcggacgac ggcgcagcca tccggcgccg gcggtcctgt
      121 ccgcagtgca gcgggcgctt caccaccttc gagcgggtgc aactgcgcga gctgatgatc
      181 ctgaagcgca acggccgccg cacgcccttc gaccgcgaca agctggagcg gtcgctgaac
      241 gtcgcgttgc gcaagcgtcc ggttcaggcg gagcaggtcg aacagctggt cagccgcatc
      301 acccgccagc tggaaagcct gggcgagacg gaaatcccgt cctcgaccgt cggcgacttc
      361 atcatgaagg cgctgaagtc ggtcgatgag gtcgcctatg tccgctacgc ctcggtctac
      421 aaggacttcc gccacaccgg cgatttcgcc cggttcctgg gcgacgaggg tctggacgaa
      481 agctgatgcg cgtcaccctg aagctggcga cgtcgctgga cggcaggatc gcgacggcct
      541 cgggtgaaag ccagtggatc acgggcgagg ccgcgcggct ggaggggcac cgtctgcggg
      601 ccgcccatga cgccattctg gtgggggtcg agaccgtgtt ggccgacgat ccggagttga
      661 ccgcgcggtt gccgggtcgt ccggtcgacc agcccctgcg ggtcgtcctc gacagccggt
      721 tgcgcacccc gccgactgcc aaggtcgcga gcggcgacag cctgatcctg accgtcgctg
      781 aacctgcgcc gatcggaacc gccgtggtcg aacgcgtcga ggccgatcat ggccgaccgt
      841 ctgtcgcggc ctgcctgtca gccttgaagc gccggggtgt gtcgtcggtg ctgatcgagg
      901 gcggcgggca ggtggcggcc agcttcctgc gcgccggcgt ggtggaccgg ctggagtggt
      961 ttcgggctcc gatcctgctg ggcggtgaag ggcggccttg cgtcgcttcg ctcgcgctgg
     1021 cgcggctttc cgacgccccc gttttccggc gccttgcggt cgagccttgt ggtgacgacc
     1081 tgtgggaacg ctacgagagg atctgaagtg ttcacgggca tcgtcaccga catcggccgc
     1141 gtccgtgcgg tcaggcagac cgagcgcgac cgccgctatg agatcgagac ggcgtgggac
     1201 accgcgggca tcgatctggg cgcatcggtc agccacgccg gctgctgcct gacggtcacc
     1261 gagaaggggc cgggctggtt cgcggtcgag gtctcgggcg agacgctgtc gaagaccagt
     1321 ctgggcgact gggccgaggg ccatcgggtc aatctggagc gggccgcgaa actgggcgac
     1381 gagatgggcg gacatgtcgt gtcgggccat atcgacggac tgggccgcgt ggcggccgtc
     1441 gtgccggagg gcggatcgca ccgggttcac atcgaggttc cggccccgct gcaccgtttc
     1501 atcgcgccca agggctccat caccgtggac ggcgtgtcgc tgacggtgaa cggggtcgag
     1561 gggcagcggt tcgacgtcaa cctgatcccc cacacctggg atgcgacgac cttgggcggt
     1621 ctgaaggtgg gcgatgcggt gaatctcgag atcgacatgc tggcgcgata cctcgcgcgg
     1681 tggcaggaga cggcgtgatg caattggccg cgaacatgaa cgacagcccg atcagcccta
     1741 tcgaggacat cctcgaggat gcccgcaacg gcaagcccta catcctcgtg gacgccgagg
     1801 atcgcgagaa cgagggcgac atcatcattc cggcccagtt tgctacgccg gaccagatca
     1861 acttcatggc ccgccatgcg cgaggcctga tctgtctggc cattacgggc gagcgcgcgc
     1921 ggcaactgcg cctgccgcct atggcagcgg aaaaccgcac cagcatgggc acggccttca
     1981 cggtttcgat cgaggcgcgc gaaggcgtga cgaccggcat ctcggccgcc gaccgcgcgc
     2041 ggacggtgca ggtcgcctcg gatccgacca agggcgccga cgacatcgtc tcgcccgggc
     2101 acgtcttccc gctggtcgcg cgcgacggc
//
LOCUS       DASVVN010000167         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_453113,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000167 DASVVN010000000
VERSION     DASVVN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453113"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="folP"
                     /locus_tag="VF686_02570"
     CDS             complement(<1..626)
                     /gene="folP"
                     /locus_tag="VF686_02570"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8660724.1"
                     /translation="MGIVNVTPDSFSDGGRLADVEAAVAHGLRLAQQGADILDIGGES
                     TRPGADPVDVDQEIARTVPVIEGLRAAGWAGPISIDTMKPDVARAAVAAGATMWNDVS
                     ALGFASDSPATAAALGCDVVLMHMKGEPRTMQDAPAYADVVAEVAEWLIARANAVMAA
                     GVARERIWLDPGIGFGKTAAHNLALTAHLDRLAATGFPVLYGASRKRT"
     gene            complement(701..1006)
                     /locus_tag="VF686_02575"
     CDS             complement(701..1006)
                     /locus_tag="VF686_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8660725.1"
                     /translation="MGLADLGRIDDVIHGRMRLGIMVYLADSDAADFTELKTVLEATQ
                     GNLSVHLKTLEKAGYVAIEKSFKDNKPLTRVSITGEGRKAFSAYLDALGSLIGGRDG"
     gene            complement(1010..1678)
                     /locus_tag="VF686_02580"
     CDS             complement(1010..1678)
                     /locus_tag="VF686_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660726.1"
                     /translation="MPAPSAKPRLHSPEDDLAFMRSIAEGGGRPPLTLAVSYLAGGLL
                     YGLQCLFHIGQATGLIRWPPLATVSFLAFITIAFLVILTWAILKDRRDGLSNRGPLAT
                     RTLSAAFSATGMANTAVIIVFGVGAYRDQDFAIWLYYAAIVFALQAAAWYMAWMLKRK
                     GWMLATAAGGWVTAVALGVLVREPLWYLGVCTVALFLLFALPGWVMFRDARTPGKVAG
                     SKAV"
     gene            complement(1740..2960)
                     /locus_tag="VF686_02585"
     CDS             complement(1740..2960)
                     /locus_tag="VF686_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8660727.1"
                     /translation="MSSSEFQQTDRLHGLDALRATALLLGVVLHASMSYFPVTIWIVP
                     DTDNSPVAAVLFFAIHLFRMTAFFLIAGLFAHMMLGRRGTLGFVRDRMIRIAGPLAAF
                     WTPVLAAIIAGLIWMAAIRNGGTIPTDGPPPPPLTVGTFPLTHLWFLYVLLILYAAML
                     IVRAPFALMDRDGAWGRFVDRMTGALIGPWTPAVLAAPLALAFWLAPNWTPFFGIPTP
                     DTALVPNPVALTAFGTAFGLGFLLDRRRDLLSRIERLWPVFTVVALGVGPAAFIMAGG
                     PVPDLAPVTAPNLKAPLAAVMALAVFASTFAALSLALRFASGYSAVRRYLADASYWIY
                     IVHLPLVMVGQILVINQTWPWFVKLGVVIAGTMGASLLTYELLVRHTFVGRWLNGRRV
                     PWRRPPQPALAPAE"
BASE COUNT          548 a         1031 c         1165 g          450 t
ORIGIN      
        1 atcgtgcgct tgcggctggc cccgtacagc actgggaagc cggtcgccgc cagccggtcc
       61 agatgcgctg tcagggccag gttgtgcgcc gccgtcttgc cgaagccgat gccgggatcg
      121 agccagatcc gttcgcgcgc gacgcccgcg gccatgacgg cattggcgcg ggcgatgagc
      181 cattccgcga cctcggcgac tacatcggca taggccggcg cgtcctgcat ggtgcgcggc
      241 tcgcccttca tgtgcatcag cacgacatcg cagccgagcg ccgccgcggt agccggactg
      301 tccgatgcaa agcccagcgc gctgacgtcg ttccacatcg ttgcgccggc ggcgacggcg
      361 gcgcgggcga cgtcaggctt catggtgtcg atactgatcg ggccggccca gccggcggcg
      421 cgcaggccct caatgaccgg aacggtgcgg gcgatttcct gatccacgtc cacggggtcg
      481 gcgcccggcc gggtgctctc gccgccgatg tcgaggatgt ccgcgccctg ctgcgccagc
      541 ctaaggccgt gcgccaccgc cgcctcgaca tcggccagcc ggccgccgtc cgagaagctg
      601 tcgggcgtga cgttgacgat gcccatgacc cgcgtcggcc cggccctggg cttcgcgccg
      661 aagatcacat ccagcgcctg ttcgatcagc cccggtccgc tcatccgtcc ctgcccccga
      721 tcaggctgcc cagcgcgtcg agataggccg agaaggcctt gcgcccctca ccggtgatcg
      781 acacccgcgt caggggcttg ttgtccttga aactcttttc gatggcgacg tagccggcct
      841 tctccagcgt cttcagatgc accgacagat tgccctgggt ggcctccagc acggtcttca
      901 gctcggtgaa gtcggcggcg tcggaatcgg ccagatagac catgatcccc agccgcatgc
      961 ggccgtggat gacgtcgtcg atccgtccga gatcggccag acccatggct cagaccgcct
     1021 tcgatccggc gactttaccg ggcgttctgg cgtcgcggaa catgacccag cccggcaggg
     1081 cgaacagcag gaacagggcg acggtgcaga cgccgagata ccacaggggt tctcgcacca
     1141 gcacgcccag cgccacggcc gtgacccagc ctccggcggc cgtggccagc atccacccct
     1201 tccgcttcag catccaggcc atgtaccagg cggcggcctg aagggcgaag acgatggcgg
     1261 cgtagtacag ccagatggcg aagtcctgat cccggtaggc gcccacgccg aagacgatga
     1321 tgaccgcggt gttcgccatg ccggtggcgc tgaaggccgc gctgagcgtg cgggtggcca
     1381 gcggtccgcg attggagagg ccgtcccggc ggtccttcag gatggcccag gtcaggatca
     1441 ccagaaaggc gatggtgatg aaggcgagga aggagacggt cgcgagcggc ggccagcgga
     1501 tcaggcccgt ggcctgaccg atgtggaaca ggcactgcag gccgtacagc aggccgccgg
     1561 ccagataaga gacggccagc gtcagcgggg gtcggccgcc accctcggcg atcgagcgca
     1621 tgaaggcgag gtcgtcctcg ggcgagtgca ggcggggttt tgcggacggg gcgggcattg
     1681 gggcgattct ccggggccgg ctcgcccatg gcgtggacga gccggcgggg cttggcaagc
     1741 tattcggcgg gcgcgagcgc gggttgcggc ggacggcgcc acgggacgcg gcgaccgttg
     1801 agccagcggc cgacgaaggt gtggcggacc agcagttcgt aggtcagcag gctggccccc
     1861 atggtcccgg cgatgaccac gcccagcttc acgaaccagg gccaggtctg gttgatcacc
     1921 agaatctgcc cgaccatcac cagcggcaga tggacgatgt agatccagta ggaggcgtcg
     1981 gcgaggtagc ggcggacggc gctgtatccg gaggcgaaac gcagcgccag cgacagggcc
     2041 gcgaaggtcg aggcgaatac ggccagcgcc atcacggccg ccagcggcgc cttcaggttg
     2101 ggggcggtca ccggggcgag gtcgggaacc ggtcccccgg ccatgatgaa cgcggccggg
     2161 ccgacgccga gagccacgac cgtgaacaca ggccacagcc gttcgatgcg gctcagcagg
     2221 tcgcggcggc ggtccagcag gaagccgagg ccgaaggccg tcccgaaggc ggtcagggcg
     2281 accggattgg gaaccagcgc cgtgtccggc gtcggaatgc cgaagaaggg cgtccagttg
     2341 ggcgccagcc agaaggcgag ggccagcggc gcggccagca cggccggggt ccacggcccg
     2401 atcagggcgc cggtcatccg gtcgacgaac cggccccagg ctccgtcgcg gtccatcagg
     2461 gcgaagggcg cgcgcacgat cagcatggcg gcgtagagga tcagcagaac gtacaggaac
     2521 cacagatgcg tcagggggaa ggtgccgact gtcaggggcg gcggcggcgg gccgtcggtc
     2581 gggatcgttc cgccgttgcg gatcgcagcc atccagatca gcccggcgat gatggcggcc
     2641 agcaccggcg tccagaaggc cgccagcggt ccggcgatgc ggatcatgcg gtcccggacg
     2701 aagccgaggg ttccccgccg gcccagcatc atatgggcga acaggccggc gatcaggaag
     2761 aaggcggtca tccggaacag gtggatggcg aagaacagga cggccgcgac cggcgagttg
     2821 tcggtgtcgg gcacgatcca gatcgtgacc ggaaaatagg acatggacgc gtgcagaacg
     2881 acacccagca gcagggcggt cgcgcgcagg gcgtcgagcc cgtgaaggcg gtcggtttgt
     2941 tggaattcag atgacgacat ggcacccctc cccttgttgg acgaacgctg tatcgcatag
     3001 actagtttat aatgcaaaca tgttcgtcat agattggttg gcatcgggga tggaggcgtg
     3061 cggagcgccg ggcctgcgcc cggagttcga tgtggtttac cgtttcgacg cagacagacg
     3121 ctccgcgcgg agattgggtc ggaagcttgc gtccggtggc ggtcttatcg ccttaccgca
     3181 gcgcctccgg cggg
//
LOCUS       DASVVN010000168         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_375251,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000168 DASVVN010000000
VERSION     DASVVN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375251"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VF686_02590"
     CDS             <1..1103
                     /locus_tag="VF686_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8660728.1"
                     /translation="VEHPVSELITGLDLVEQMIRVAAGEPLAFRQQDLHIDGWAIESR
                     IYAEDPYRKFLPSIGRLVRYEQPQEGPHEDGYVVRNDSGVREGDEISMFYDPMIAKLC
                     AWGPTRDEALTGMARALEDTHLQGVGHNIPFLSAVMDQPRFRSGNISTSYIKDEFPDG
                     FHGLKPDARQTQIMIAAAVAMNEIAAEQAGDPSDRTDWVVLIDKAPHGVDLSYDADEA
                     LLLAFTGGGHARLTEIDWRPGLAQFRAELDGEPFTAEVSRVADGYLIRHRAAKARVRV
                     LTPHTAELYARLPEKVAADTSKLIASPMPGLVVSIPVTVGQEVKTGETVAIIEAMKMQ
                     NILKAERDGVVKAVSAAAGDPVAADDVLVEFV"
     gene            1127..1672
                     /locus_tag="VF686_02595"
     CDS             1127..1672
                     /locus_tag="VF686_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX8660729.1"
                     /translation="MTLRDKLFSFDGRLRRRDWWLWSIGTGIAYFTIAMVAGPIVFGQ
                     QWGAMTDWGQASRIAPWQLATFDVVTYAPSLWIQTALATKRTHDRNNGAALAAGMTVF
                     YGLLTFGATAVDLLPSGMLTPASIETLLLTLNGVGALIGFYLFVVLGVLDGTHGPNRF
                     GPSPKGLSGETPAFMTPGGLE"
BASE COUNT          308 a          608 c          544 g          286 t
ORIGIN      
        1 aggtggagca tccggtcagc gagctgatca ccggtctcga cctggtggag cagatgattc
       61 gcgtcgccgc gggcgaacca ttggcattcc gccagcagga cctgcacatc gacggctggg
      121 cgatcgagag ccgcatctac gccgaggatc cgtaccgcaa gttcctgccg tcgatcggcc
      181 gcctcgtccg ctatgagcag ccgcaggagg gcccgcacga ggacggctat gtcgtgcgca
      241 acgattccgg cgtccgcgag ggcgacgaga tcagcatgtt ctacgacccc atgatcgcca
      301 aactgtgcgc ctgggggccg acgcgggacg aggccctgac cggcatggcc cgggcgctgg
      361 aggacaccca cctgcagggc gtcggccaca acatcccgtt cctgtcggcc gtcatggacc
      421 agccgcgctt ccgcagcggc aatatctcga ccagctacat caaggacgaa ttccccgacg
      481 gcttccacgg cctcaagccc gacgcgcgcc agacgcagat catgatcgcc gccgccgtgg
      541 ccatgaacga gatcgccgcc gaacaggccg gcgacccgtc cgaccgcacc gactgggtcg
      601 tgctgatcga caaggccccg catggcgtcg acctgtccta cgatgcggac gaggccctcc
      661 tcctcgcctt caccggcggc ggccacgccc gcctgacgga gatcgactgg cgccccggcc
      721 tggcccagtt ccgcgcagag ctggacggcg agcccttcac cgccgaggtc tcccgcgtcg
      781 ccgatggcta tttgatccgc caccgcgcgg cgaaggcgcg cgtccgcgtc ctgaccccgc
      841 acacggccga gctctacgcc cgcctgccgg aaaaggtcgc cgccgacacc tccaaactga
      901 tcgcctcccc catgcccgga ctggtcgtct cgatcccggt gaccgtgggc caggaggtca
      961 agaccggcga aaccgtcgcc atcatcgagg ccatgaagat gcagaacatc ctcaaggccg
     1021 aacgcgacgg cgtggtgaag gccgtcagcg ccgccgccgg cgatcccgtg gccgccgatg
     1081 acgtgctggt ggagttcgtc taactctcat cgagggggat tcgaacatga cgttgcgcga
     1141 caagctgttc agcttcgacg gacgactgcg ccgcagggat tggtggctct ggtccatcgg
     1201 aacagggatc gcctatttca ccatcgccat ggtcgccgga ccgatcgtct tcggccagca
     1261 gtggggggcc atgactgatt ggggccaagc cagccgcata gcgccatggc aattggcgac
     1321 ttttgacgtc gtgacatacg cgccctcgct ctggattcag acggccttgg ccacaaaacg
     1381 aactcacgac aggaacaacg gcgcggcgct ggcggccggc atgacggtct tctatggcct
     1441 gctcaccttc ggagccacgg cggtcgacct ccttccgagc ggtatgctca cgccggcctc
     1501 gatcgaaact ttattgctga cgctcaacgg cgtgggggcc ttgatcggct tctatctgtt
     1561 tgtcgtcctc ggcgtgcttg atggcacgca cggacccaat cgcttcggcc cctcgcccaa
     1621 gggactttct ggcgaaacac ccgccttcat gactcctggc ggactggagt agtgacctca
     1681 gcccgttccg gtggccgcgt aaatcgaaga ccgcgcagca aagggtcgct gtagttgaag
     1741 cccacc
//
LOCUS       DASVVN010000169         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_164727,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000169 DASVVN010000000
VERSION     DASVVN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164727"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..>1577)
                     /locus_tag="VF686_02600"
     CDS             complement(303..>1577)
                     /locus_tag="VF686_02600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8660730.1"
                     /translation="DIGVEARIAAGVLLNAALEGRGGLDAALSFREVSSLPGPDRAFA
                     RAVAMAALRRLGEIDQILDRKLQKGPPLAVRTILRVALAQMLILETPAFAAVSTAVKL
                     AEREPKTRPYKNLVNAVLRGIDREGPGLTTAESNLPEWLSARWRAAYGEAALVGLALA
                     AREEPATDLSIKPDVDAAVVAEAVEGEVLPGGSIRTGRRGDLATWPGFDDGVWWVQDA
                     AASVPGRLLAPKAGESVIDLCAAPGGKTLQLAASGASVVALDRSAVRLKRLTENLART
                     DLEAEVVATPAEDWEDGRSFDAVLLDAPCTATGTFRRNPEVLRATKPADVAKLADVQH
                     RLLDAAAERVKPGGRLVYCVCSLEREEGETQIIAFLRRNAAFKTAPADPAAVGAPDDA
                     LTPEGWLRILPSMWAEKGGVDGFFVARLDRVG"
BASE COUNT          208 a          599 c          554 g          216 t
ORIGIN      
        1 gtcgctcgcc ggggtcagat ccgcctgcct tgaaatcctc agggctcgcc gcccatcctg
       61 atccatgccc ggcgtgttcg agccccgtct gcggggcacg ctccatgcag cgatgcccgc
      121 gccggggccg acgcctgcag gaccttcgaa ttcagcacaa atccggcgcc gccggcgccg
      181 gggacccgga cgaccttgct ccaaggcccc ctggaccgca cagcacggag aagcccccag
      241 ggcgttcgcc gttcattggc gagacctcgt tggcgatgct gcgccagccg gcccgccctt
      301 cgtcagccga cccgatccag cctggcgacg aagaagccgt ccaccccgcc cttttccgcc
      361 cacatcgacg gcaggatgcg cagccagccc tcgggcgtca gcgcatcgtc cggcgcgccg
      421 acggcggccg ggtcggcggg cgcggttttg aaggccgcat tccggcgcag gaaggcgatg
      481 atctgggtct cgccctcctc gcgctccagc gaacagacgc aatagaccag ccgtccgccc
      541 ggcttcaccc gttcggcggc ggcgtccagc agacggtgct ggacgtcggc cagcttggcg
      601 acgtcggcag gcttggtggc gcgcagcact tcggggttgc ggcggaaggt gccggtggcc
      661 gtgcagggtg cgtcaagcag gacggcgtcg aagctgcggc cgtcttccca gtcctcggcg
      721 ggggtggcga cgacctcggc ctcaaggtcc gtacgcgcca gattctccgt caagcgtttc
      781 agccgcaccg cggagcggtc cagcgccacg acgctggctc ccgacgccgc cagttgcagc
      841 gtcttgccgc ccggcgcggc gcacaggtcg atgacggatt ctccggcctt tggcgccagc
      901 aggcgcccgg gcacggacgc cgccgcgtcc tgcacccacc agacgccgtc gtcgaagccc
      961 ggccaggtcg ccagatcacc gcgccggccc gtgcggatcg agccccccgg caggacctcg
     1021 ccctcgaccg cctcggccac cacggcggcg tcgacgtcgg gtttgatgct gaggtccgtc
     1081 gccggctcct cgcgcgccgc cagcgccagc ccgaccagcg cggcctcgcc ataggcggcg
     1141 cgccagcggg cggacagcca ttcgggcaga ttgctctcgg ccgtggtcag gcccgggccc
     1201 tcgcggtcga tgccgcgcag caccgcgttg accagattct tgtagggccg cgtcttgggc
     1261 tcgcgctcgg ccagtttcac ggccgtcgac accgccgcga aggccggggt ctcgaggatc
     1321 agcatctggg cgagggcgac gcgcaggatg gtgcgcaccg ccagcggcgg gcccttctgc
     1381 agcttgcggt cgaggatctg gtcgatctcg cccagccggc gcagggccgc catggccacg
     1441 gcgcgggcga aggcccggtc ggggcccggc aggctggaga cctcgcggaa cgacagggcg
     1501 gcgtccagcc cgccccggcc ttccagcgcg gcgttgagca gcacgccggc cgcgatgcgc
     1561 gcctcgacgc cgatgtc
//
LOCUS       DASVVN010000170         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_295314,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000170 DASVVN010000000
VERSION     DASVVN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295314"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF686_02605"
     CDS             complement(<1..372)
                     /locus_tag="VF686_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8660731.1"
                     /translation="MTLRIRRLELTPAERRAAARGNGALALVPRLKADGWRLTATQWL
                     SSRFGATAARVTAAQLKRRGVRVETLRIPSEDGGVSIRILNPSRPPRGVVLDMHGGGW
                     VIGSAGLNDHLNADLAAEAGLA"
     gene            complement(425..>2233)
                     /locus_tag="VF686_02610"
     CDS             complement(425..>2233)
                     /locus_tag="VF686_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX8660732.1"
                     /translation="PLVALKNVRLQDGQRPLFDGVDIAVEPRTRAALVGRNGAGKSTL
                     MKMVMGLIEPDAGDRSVQAGTRFAYVPQEPVITGETLLDYAASEGAERWTAEAWLTTF
                     GLDPEKAAVNLSGGETRRAALAKAFAEEPDLLLLDEPTNHLDILAIELLEEELLQARF
                     SVLVVSHDRAFLNRVTQSCHWLEGRRVRTLNKGFAAFDEWASKVLEEEAQSLQRLTKT
                     IERETETFYRSITARRTRNEGRARSLDALRADRNERLKDVPRELNLGVDSGATSGKLV
                     ADLKGVSKSFGARPVIKPFTTRVIRGDRLAIVGPNGAGKTTLVKILLGELAPDEGTVK
                     LGANLEPVYLDQSREGLRSDMTLWDALTPGGGDSVLVRGISKHVAAYAKDFLFQEGQL
                     RQPISTLSGGERNRLLLARALAKPANMLVLDEPTNDLDMDTLDKLEELLESYDGTLIL
                     VSHDRDFVDRLATSTIAMNGRGDIVETPGGWTDFIRQNPGFLQPGANPRPQDKEAARR
                     AAAAPAPASVSAPKKPGKLTFKDAHRLKELEGLLASLPGDIARHDATLSDPDLYARDR
                     KAFDRATANADRARALLAAAEEEWLELEAKREALSA"
BASE COUNT          314 a          697 c          850 g          372 t
ORIGIN      
        1 cgccagaccg gcttcggcgg cgaggtcggc gttcaggtgg tcgttgagcc cggcgctgcc
       61 tatcacccag ccgccgccgt gcatgtccag cacgacgccg cgcggcgggc gggaagggtt
      121 gaggatgcgg atggagacgc cgccgtcttc ggacgggatg cgcagggtct ccacccgcac
      181 ccctcgccgc ttgagctgag cggcggtcac ccgggcggcg gtggcgccga accggctgct
      241 gagccattgg gtggcggtca atcgccagcc gtcggccttg aggcgcggga ccagcgccag
      301 cgccccattg ccccgcgcgg cggcgcggcg ttcggccggg gtcagctcca ggcggcgaat
      361 gcgaagggtc atgggcgaag gctagcccag ctgccggttg caggcgatgc ggcgttctgg
      421 ccggtcaggc cgacagggct tcccgcttcg cctcaagttc cagccactcc tcctccgccg
      481 ccgccagaag ggcgcgggcg cggtcggcgt tggccgtggc gcggtcaaac gccttgcggt
      541 cccgggcgta gaggtccggg tccgacaggg tcgcgtcgtg gcgggcgatg tccccgggga
      601 ggctggcgag caggccctcc agctccttga ggcgatgggc gtccttgaag gtcagcttgc
      661 ccggcttctt cggagcggaa acggaggcgg gcgccggagc ggcggcggca cgccgggcgg
      721 cttccttgtc ttgcggccgg gggttggcgc cgggctggag gaagccgggg ttctgacgga
      781 tgaagtcggt ccagccgccg ggcgtctcga cgatgtcgcc ccggccgttc atcgcgatgg
      841 tcgaggtggc cagacgatcg acgaagtcgc gatcgtggct gacgaggatc agggtgccgt
      901 cgtaggactc gagcagctct tcgagcttgt cgagcgtgtc catgtcgagg tcgttggtcg
      961 gttcatcgag gaccagcata ttggcgggct tggcgagcgc ccgggccaga aggaggcggt
     1021 tgcgttcacc gcccgaaagc gtcgagatcg gctggcgcag ctggccttcc tggaacagga
     1081 aatccttggc gtaggcggcc acgtgtttgg agatgccgcg cacgaggacg ctgtcaccgc
     1141 cgcccggggt cagagcgtcc cacagggtca tgtcggaccg caggccctcg cgggactggt
     1201 cgagatagac aggttcgaga ttggctccga gcttgaccgt gccttcgtca ggcgcgagct
     1261 cgccgagcag gattttgacg agggtggtct tgcccgcgcc attcgggccg acgatggcca
     1321 gccggtcgcc gcgtatgaca cgggtggtga agggcttgat gaccggccga gcgccgaagc
     1381 tcttcgaaac ccccttcagg tccgccacca gcttgccgga ggtggcccct gaatcgaccc
     1441 cgagattgag ttcgcggggg acgtccttga gccgttcgtt gcggtcggcc cgcagggcgt
     1501 ccagcgagcg cgcgcgtccc tcgttacgcg tacggcgggc ggtgatcgag cggtagaagg
     1561 tctcggtttc gcgctcgatg gtcttcgtca ggcgctgaag ggattgggct tcctcctcca
     1621 ggaccttgct cgcccattcg tcgaaggcgg cgaagccctt gttgagggtg cggacccggc
     1681 ggccctccag ccagtggcag gactgggtga cgcggttcag gaaggcgcgg tcgtggctga
     1741 cgaccagaac cgagaaccga gcctgcagga gttcttcctc gagcagttcg atggctagga
     1801 tgtcgaggtg attggtcggc tcgtcgagca gcagcaggtc gggctcttcg gcgaaggcct
     1861 tggcgagcgc ggcgcggcgg gtttcgccac ccgacaggtt gacggcagcc ttttccgggt
     1921 cgaggccgaa ggtggtcagc caggcctcgg cagtccagcg ctcggcgcct tccgaggcag
     1981 cgtagtcgag cagggtttcg ccggtgatga cgggttcctg cggcacatag gcgaagcgcg
     2041 tgccggcctg gaccgaacgg tcgcccgcat cgggctcgat caggcccatg accatcttca
     2101 tcagggtcga tttgccggcc ccgttgcggc ccacgagcgc ggcgcgggtg cgcggctcga
     2161 ccgcgatgtc gacgccatcg aacagcgggc gctggccatc ctggaggcgg acgttcttga
     2221 gagcaacgag agg
//
LOCUS       DASVVN010000171         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_400818,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000171 DASVVN010000000
VERSION     DASVVN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400818"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF686_02615"
     CDS             <1..294
                     /locus_tag="VF686_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX8660733.1"
                     /translation="WPWATITANVAGGLLMGLLAGWLAFRGGAGESLRLFAAVGVLGG
                     FTTFSAFSLETALMIERREFALAGGYVAASVALSVLALFIGLMVARRAFGAAL"
     gene            291..1325
                     /locus_tag="VF686_02620"
     CDS             291..1325
                     /locus_tag="VF686_02620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8660734.1"
                     /translation="MTDEPIKDAEPAPKREVLTIYVAEAEDGIRLDRWFRRRWPHLSN
                     IQVQKMARSGQIRVDGARIKPEGRLTAGAAVRVPPIPDDTSRQAGDVHTLSPQDIKFA
                     KSLVLYEDDMVIAINKPHGLAVQGGTKTSRHVDRLLSAWGEGMDRPRLVHRLDRDTSG
                     VLLLGKGPEAAKRLAGAFARRQAKKTYWAIVIGNPKPSAGQIDMPLKKTGINDFEMMR
                     PADRKDPRAEPAETAFATISRAAHRASWMALRPFTGRTHQLRAHMAGIGHPILGDPKY
                     GDDKSKELSGPLKLQLHARSIELEHPRGGMLKVEAPLSAEMKAGFAHFGFEEAEGNES
                     DPFEGVRRQR"
     gene            1474..2151
                     /locus_tag="VF686_02625"
     CDS             1474..2151
                     /locus_tag="VF686_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8660735.1"
                     /translation="MTRPLAVFDIDGTLVDSRASIHRAACEAARDIGLPEPDYDRVRQ
                     IVGLSLEHAMAVLEPGLDPPTLARFVAGFRASFNRMYAEGHEEPLYPEAMTTLRRLHR
                     DGWRLALATGQNRRGVARNLAREGWADLFLSSHCAEDGPGKPDPAMLHAAMAACDSDP
                     SRTVMIGDTAHDIEMAINAGVLPLGVAWGFHTIEEQQAAGARHVARSFSELTVVLDGF
                     ADLTRAA"
     gene            2160..2924
                     /locus_tag="VF686_02630"
     CDS             2160..2924
                     /locus_tag="VF686_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX8660736.1"
                     /translation="MSHIPPLDPNVAARKGFRESEERLKRFWTTADVKQVETGWAVTL
                     DGRTPKTPAHAQLILPTEAAARLVAEEWAAQGEFIDPATMPATRLASTAIDRVSQARE
                     PVAEEIAAFAGSDLLCYLADHPTSLAVEQAREWTPWRDWAAVELGVHLEPAEGVIHRA
                     QDPAAIARVQELALRLDDFALTGLATAVPLLGSAVLGLAVQRGVLSGEAAFELSRLDE
                     AFQERQWGVDEEAAERTAGRRAEAVMLDRWFKALAA"
     gene            2963..>3194
                     /locus_tag="VF686_02635"
     CDS             2963..>3194
                     /locus_tag="VF686_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8660737.1"
                     /translation="MKTILEQLEQRRAGARLGGGETRIASQHAKGKLTARERIDVLLD
                     EGSFEETDMFVEHRSTQFGMEKQRIPGDGVVTG"
BASE COUNT          491 a         1248 c         1010 g          445 t
ORIGIN      
        1 tggccgtggg cgacgatcac ggcgaacgtc gcgggcggcc tgctgatggg cctgctggcc
       61 ggctggctgg ccttccgggg cggggcaggc gaaagcctcc gcctgttcgc cgcggtcggc
      121 gtactgggcg gcttcaccac cttctcggcc ttctcgctgg aaaccgccct gatgatcgag
      181 cgccgtgaat tcgccttggc gggcggttac gtcgccgcct cggtggcgct ctcggtgctc
      241 gccctgttta tcggcctgat ggtcgcccgc cgcgccttcg gagccgccct ttgactgacg
      301 aacccatcaa ggacgccgaa cccgcgccga agcgcgaggt cctgaccatc tacgtcgccg
      361 aggctgagga cggcattcgt ctcgaccgct ggttccgccg tcgctggcct cacctgtcca
      421 acatccaggt gcagaagatg gcccgcagcg gccagatccg cgtcgacggc gcccgcatca
      481 agcccgaagg ccgcctgacc gccggcgccg ccgtgcgcgt gcccccgatc cccgacgaca
      541 cctcgcgtca ggccggcgac gtccacacgc tcagcccgca ggacatcaag ttcgccaagt
      601 cgctcgtgct ctacgaggac gacatggtga tcgccatcaa caagccgcac ggcctcgccg
      661 tccagggcgg caccaagacc agccgccacg tcgatcgcct gctgtcggcc tggggggagg
      721 ggatggaccg cccgcgtctc gtccaccgcc tcgaccgcga cacctccggc gtcctgctgc
      781 tgggcaaggg ccccgaggcg gccaagcgtc tggccggcgc cttcgcccgg cgtcaggcca
      841 agaagaccta ctgggccatc gtcatcggca atcccaagcc ctcggccggc cagatcgaca
      901 tgccgctgaa gaagaccggc atcaacgact tcgagatgat gcgtcccgcc gaccgcaagg
      961 acccgcgcgc cgaaccggcc gagaccgcct tcgccactat ctcccgcgcc gcccatcgcg
     1021 cctcgtggat ggccttgcgc cccttcaccg gccggaccca ccagctgcgc gcccacatgg
     1081 ccggcatcgg ccacccgatc ctcggcgacc ccaaatacgg ggacgacaag tcgaaggagc
     1141 tgtccggtcc gctgaaactg cagctccacg cccgcagcat cgagctggaa cacccgcgcg
     1201 gcggcatgct caaggtcgag gctccgctga gcgccgagat gaaggccggc ttcgcccatt
     1261 tcggtttcga ggaagccgag ggcaacgaga gcgatccgtt cgaaggggtg agacggcagc
     1321 gatgagccca tttagtccgt gcatccccgc gaaggcgggg acccaggttt cggctacgcc
     1381 ccagctgtcg gaagacgccg ccgccggaac acgcaccgcc cctgccgccg ctcaaacctg
     1441 ggtccccgcc ttcgcgggga tgcacggtgt taggtgaccc gccccctcgc agtcttcgac
     1501 atcgacggca ccctcgtcga cagccgcgcc tcgatccacc gcgccgcctg cgaggccgcg
     1561 cgcgacatcg gcctgcccga gcccgactac gaccgcgtcc gccagatcgt cggcctgtcg
     1621 ctggagcacg ccatggccgt tctggagccg ggcctcgacc ccccgacgct ggcccggttc
     1681 gtcgccggct tccgcgccag cttcaaccgc atgtacgccg agggccatga ggagccgctc
     1741 tatcccgagg ccatgaccac cctgcgccgc ctgcaccgcg atggctggcg gctggccttg
     1801 gccacgggcc agaaccgccg cggcgtggcg cgcaatctgg cgcgcgaggg ctgggccgac
     1861 ctgttcctct cgtcccactg cgccgaggac ggccccggca agccggatcc cgccatgctc
     1921 cacgccgcca tggccgcctg cgacagcgat ccctcgcgca ccgtcatgat cggcgacacc
     1981 gcccatgaca tcgagatggc catcaacgcc ggcgtcctgc cgctgggcgt cgcctggggt
     2041 ttccacacca ttgaggaaca gcaggccgcc ggcgcccgtc acgtcgcccg gtccttctcc
     2101 gaactgaccg tggtgctgga cggcttcgcc gacctgaccc gcgccgcctg agacccaaga
     2161 tgagccacat cccgcctctc gatcccaatg tcgccgcccg caaaggcttc cgcgagtccg
     2221 aggaacgcct gaaacgcttc tggaccaccg ccgacgtcaa gcaggtcgag accggctggg
     2281 cggtcactct cgacggccgc accccgaaaa cccccgctca cgcccagctc atcctgccga
     2341 ccgaagccgc cgcgcgtctc gtggccgagg aatgggccgc ccagggcgaa ttcatcgatc
     2401 ccgcgaccat gcccgccacc cgcctggcct ccaccgccat cgaccgcgtc agccaggcgc
     2461 gcgagcccgt ggcggaagag atcgccgcct tcgccggctc ggacctgctc tgctacctcg
     2521 ccgaccaccc cacctcgctg gctgtcgaac aggcccgtga atggacgccg tggcgcgact
     2581 gggccgctgt ggagctgggc gtgcatctcg aaccggctga aggcgtcatc catcgggcgc
     2641 aggatcccgc cgccatcgct cgcgtgcagg agctggccct gcgcctcgac gacttcgccc
     2701 tgaccggcct cgccaccgcc gtgcccctgc tgggctccgc cgtcctcggc ctcgccgtcc
     2761 agcgcggcgt cctgtcgggc gaggccgcct tcgagctcag ccgtctcgac gaggccttcc
     2821 aggaacgtca gtggggcgtc gacgaggagg ccgccgaacg caccgccggc cgccgcgccg
     2881 aggccgtgat gctggaccgg tggttcaagg cgcttgccgc ctgagccgtc gctccttaag
     2941 ccagcgctca aggagacgcc gcatgaagac catcctcgaa cagctggaac agcgccgcgc
     3001 cggcgcccgc ctcggcggcg gcgagacacg catcgccagc cagcacgcca agggcaagct
     3061 gaccgcccgc gaacgcatcg acgtcctgct cgacgagggc tccttcgagg aaaccgacat
     3121 gttcgtcgag caccgctcga cccagttcgg catggagaag cagcgcatcc ccggcgacgg
     3181 cgtggtcacc ggcc
//
LOCUS       DASVVN010000172         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_138226,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000172 DASVVN010000000
VERSION     DASVVN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138226"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF686_02640"
     CDS             <1..409
                     /locus_tag="VF686_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEX8660738.1"
                     /translation="TIWVPASYVTDFASIPQIGRWLIPPFGRHAIAAVVHDWLYSVGE
                     PGKRGEADDIFREALTELGVDMARRNIMHGAVTAFGAGGYDRAEATWPTQFMDWRTGK
                     YLPPPAPRAAFFGQGDPAVIEWVKSQSAANCGP"
     gene            complement(380..1015)
                     /locus_tag="VF686_02645"
     CDS             complement(380..1015)
                     /locus_tag="VF686_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX8660739.1"
                     /translation="MADPAHSLDEQVRAADPDRWLSSRFVADERLRADLIALYAFEAE
                     LLAIPTRVTQPLLAEMRYVWWREQMDGVFAGVPRRGHPVLEALTDIVARHGLDRAPFD
                     ALIEAHIGRVHGEPHDLDAFYVGPMQTAVRVLGAENPHHAVPAGRAWGLMQTGRFEEA
                     RAALPDAARTARTLPVGAFPAVLPATLARRPDAGPTQKRLRLLWAAVRGRL"
     gene            complement(1008..1628)
                     /locus_tag="VF686_02650"
     CDS             complement(1008..1628)
                     /locus_tag="VF686_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660740.1"
                     /translation="MGKLLSNFRTTILLSLVLAGVMILSFGRIAPGGFDQSFWQAVFR
                     WVHVAAGILWIGLLYYFNFVQIRVMPGIPAELKPAIGKHLAPEALFWFRWSALVTVLA
                     GLAVMFTRGHAYAAEVLTFGLAQGLTEDQKGFTLMGIGVWLAIIMFLNVWGIIWPNQK
                     RALGIVPAEDDVKAKSARIAMLASRTNLLLSLPMLTSMAMYQTLFG"
     gene            complement(1797..>2773)
                     /gene="secF"
                     /locus_tag="VF686_02655"
     CDS             complement(1797..>2773)
                     /gene="secF"
                     /locus_tag="VF686_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8660741.1"
                     /translation="PLIRLLPQKTNFKFVSYARYAAALSVVLCVASIVACFYPGLNMG
                     IDFRGGASMEVSKPAGQVIELERVRSAANGLDLGDVQVQGIARRDTNVDDGSTAILRF
                     QVPEGRDQSAVVNQVESAITGAVGEVTYSGVSVVGSKVSGELFQSGLMALGVAILLMF
                     AYIWFRFEPQFGFGAIAGLLHDVILTFGLIVLFKLEFSLTMVAAILTVIGYSMNDTVV
                     VFDRLRENLRKYKTMPLREVIDLSLNETLSRTVITGITAVMVLAALAYFGGEALFGFS
                     IALMFGIIVGTYSSIYVGAPIILLWGVKRGSRDKDDLKPVKLGMASRP"
BASE COUNT          493 a          950 c          925 g          405 t
ORIGIN      
        1 gaccatctgg gtcccggcct cctacgtcac cgacttcgcc tccatcccgc agatcggccg
       61 ctggctgatc ccgccgttcg ggcggcacgc catcgccgcc gtcgtccacg actggctgta
      121 ttcggtcggc gagcccggca agcgcggcga ggccgacgac attttccgcg aggccctcac
      181 cgaactgggg gtcgacatgg cccgccgcaa catcatgcac ggggcggtga ccgccttcgg
      241 cgccggcggc tacgaccggg ccgaggcgac atggccgacg cagttcatgg actggcgcac
      301 gggcaaatac ctgcccccgc ccgcgccgcg cgcggccttc ttcggccagg gcgaccccgc
      361 ggtcattgaa tgggtgaagt cacagtcggc cgcgaactgc ggcccatagc agccgtagcc
      421 gcttttgcgt cggccccgcg tccggacgac gggccagggt cgcgggcagg accgccggaa
      481 acgccccgac tggcagcgtc cgcgccgtcc gggccgcatc cggcaacgcc gcgcgggcct
      541 cctcgaaccg gccggtctgc atcaggcccc aggcccgtcc cgccgggacc gcatggtgcg
      601 gattctctgc gcccaatacc cgaaccgccg tctgcatcgg gccgacgtag aaggcgtcca
      661 gatcgtgcgg ctcgccatgg acccggccga tatgggcttc gatcagggcg tcgaaggggg
      721 cgcggtccag cccgtgccgg gcgacgatat cggtcagggc ttcgagaacc ggatggcccc
      781 tgcgcgggac gccggcgaac accccgtcca tctgctcgcg ccaccagacg tagcgcatct
      841 cggccagcag gggctgggtc acccgcgttg gaatggccag cagttcggcc tcgaaggcgt
      901 agagggcgat caggtccgcc cgcagccgct catcggccac gaagcggctg gacagccacc
      961 ggtcgggatc ggcggcgcgg acctgttcgt cgagactgtg ggccggatca gccaaacagg
     1021 gtctggtaca tcgccatcga cgtcagcatc ggcagcgaca gcagaaggtt ggtgcggctg
     1081 gccagcatgg cgatacgggc ggatttggct ttcacatcgt cctctgccgg aacaatgccc
     1141 agggcccgct tctggttcgg ccagatgatg ccccagacgt tgaggaacat gatgatggcc
     1201 agccagacgc cgatgcccat cagggtgaag cccttctgat cctcggtcag gccctgcgcc
     1261 aggccgaagg tcagcacctc ggcggcgtag gcgtggccgc gggtgaacat gacggccagg
     1321 ccggccagca ccgtcaccag cgccgaccaa cggaaccaga acagggcctc gggggcgaga
     1381 tgtttgccga tcgccggctt cagctcggcc ggaatgccgg gcatgacccg gatctggacg
     1441 aagttgaaat agtacagcag cccgatccac agaatcccgg ccgcgacgtg gacccaccgg
     1501 aacaccgcct gccagaagct ctggtcgaag ccgccgggcg cgatgcggcc gaacgacagg
     1561 atcatcaccc ccgccagcac gaggctcagc aggatggtgg tgcggaaatt cgacagcagc
     1621 ttgcccatgg tcagcccctc ccagagcttc gattcggtga agcggcacca tagctctccc
     1681 tccccttcat ggggagggtg gtcgcgcagc gaccgggtgg ggaatgtctg acccacccca
     1741 ccctgtcgtc gcttcgcgcc gacatccctc cccatgaagg ggagggagat agccgcctag
     1801 ggccgcgagg ccatgcccag cttgaccggc ttcagatcgt ccttgtcgcg gctgccgcgc
     1861 ttgacgcccc acagcaggat gatcggcgcg ccgacataga tcgacgaata ggtgccgacg
     1921 atgatcccga acatcagggc gatcgagaag ccgaacaggg cctcgccgcc gaagtaggcc
     1981 agggcggcca gcaccatcac cgcggtgatg ccggtgatga cggtccgcga cagggtctcg
     2041 ttcagcgaca ggtcgatcac ctcgcgcagg ggcatggtct tgtacttgcg caggttctcg
     2101 cgaaggcggt cgaacaccac cacggtgtcg ttcatcgaat agccgatgac ggtcaggatg
     2161 gccgccacca tggtcaggct gaactccagc ttgaacagca cgatcagtcc gaaggtcagg
     2221 atcacgtcgt gcagcaggcc ggcgatcgcg ccgaagccga actgcggctc gaaccggaac
     2281 cagatgtagg cgaacatcag caggatcgcg acgcccagcg ccatcagccc cgactggaac
     2341 agctcgcccg agaccttcga gccgacgacg ctgacgcccg aataggtcac ctcgccgacg
     2401 gcgccggtga tggcgctctc gacctggttg acgacggcgc tctgatcccg gccttccggc
     2461 acctggaagc gcaggatggc ggtggatccg tcgtcgacat tcgtgtcgcg gcgggcgatg
     2521 ccctggacct gcacgtcgcc caggtccaga ccgttggcgg cgctgcgcac acgttccagc
     2581 tcgatcacct gcccggcggg tttggacact tccatcgagg cgccgccccg gaagtcgatg
     2641 cccatgttca ggccgggata gaagcaggcg acgatcgacg ccacgcacag cacgaccgac
     2701 agggcggcgg cgtagcgggc gtagctgacg aatttgaagt tcgtcttctg cggcagcagt
     2761 cggataaggg gcc
//
LOCUS       DASVVN010000173         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_347961,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000173 DASVVN010000000
VERSION     DASVVN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347961"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..912
                     /gene="prmC"
                     /locus_tag="VF686_02660"
     CDS             19..912
                     /gene="prmC"
                     /locus_tag="VF686_02660"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX8660742.1"
                     /translation="MTETSPAPTLVTAWKAATATLKAGRIDSPSIDARLLLEVATGAT
                     RTDILADPYRAVTVDQQAALDAYVERRLRREPVSRILGKKGFWKIMLTVTPDVLSPRP
                     DTEAILDVVMLAYPPNRAFEMIDLGTGSGAILLATLAERAGARGVGTDISSEALAVAK
                     ENAANLELDNRATFLRTEWAAGFGDDSFDLVVSNPPYIPSGDIPGLDPEVRDHDPLLA
                     LDGGPDGLVAYRELAPEIRRILRPGGVFAVEIGWDQGPQVKALFEAAGFEGVIVVKDL
                     SDRDRVVTNGPDPRTNPIPKM"
     gene            1193..2164
                     /locus_tag="VF686_02665"
     CDS             1193..2164
                     /locus_tag="VF686_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX8660743.1"
                     /translation="MRDFKGMKRQRGRNRKPGSGGGNNNANATNPNRSWDSQGPENIK
                     VRGNAQTVYERYMQLARDASAAGDRVLAENYTQHAEHYFRVLRALQPSRPVSEIAQRE
                     LAGQGFDIDFEDETGAQAAAIMAAEKVEADRLAQIEARQRENEERSNDRQGDRQGGEQ
                     PRREWRDRDDQPRADREPREPREPRPEGEASTNPDGSRRETRRERWERRQLERNARFD
                     REGGERPEGDAPPSDEAPEARAETVERPERPEREERLARAERPERRPRRDAGVAADDG
                     PSALPGFLTRSAAPAPVEAPAAAETSEEAPAPKRRAPRRKVEAPTED"
     gene            2246..2893
                     /locus_tag="VF686_02670"
     CDS             2246..2893
                     /locus_tag="VF686_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEX8660744.1"
                     /translation="MTRLSRSPALIALAALALTACASAPPPPPPPSGTGAALRDWRGI
                     VTSVDRDRYARRDAAWSLALQQARRQTGSGDLRALGDLIDPDIRRAPVAPPPGAYRCR
                     TVKLGSQGGEQGLGYVVYGWFACRIEQTPRGLKFSKLTGSQRPSGLLFPENDRQMVML
                     GSLALAEEPAANSYGQRPDRDLIAVLERIGDRRWRLVLPWPQAESNLDVIELVPA"
     gene            2890..4218
                     /locus_tag="VF686_02675"
     CDS             2890..4218
                     /locus_tag="VF686_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8660745.1"
                     /translation="MTKTPAPKPRKPRAKAASGREVPKGRRPRPTRKAPEALDIPPPP
                     RRARPADIAVIGAGPTGLAVALMLVRQGRRVMVHERFARPHPIGAGFLLQPTGLHVLD
                     GLGLTPQVEAVGQRIDHIFGREARRGRVVLDIRYSDLKRPRQAIGIHRAAMFDILYSA
                     CVEAGVDFETDREIVAADAGHLIDSGGRRSATPFNLVIDASGSRSAIAAGMGEERRDL
                     AWGALWTTVPWPGAPFDPTALEQVYLGASRMLGVLPCGRLPDDPAPLATFFWSLKTEI
                     WADWHTHGLDAWKAHCLRLWPELMPVMDAITDREQMTLASYGHQTLARPVGDRLAIVG
                     DAAHSTSPQLGQGVNMGLLDALALTGALRQHRDLPTALNAYARARRWHVRLYQALSLG
                     FTPFYQADGRLLPWFRDHVLGKVARLPLAPRLLAATVAGVLLDPRSSGSG"
BASE COUNT          644 a         1735 c         1335 g          537 t
ORIGIN      
        1 ccgcgccaat gccagcgcat gaccgaaacc agccccgccc cgacccttgt caccgcctgg
       61 aaggccgcca ccgcgaccct caaggccggc cgcatcgaca gcccctcgat cgacgcccgc
      121 ctgctgctgg aagtcgccac cggcgcgacc cgcaccgaca tcctcgccga cccctaccgc
      181 gccgtcaccg tcgaccagca ggccgcgctg gacgcctatg tcgaacgccg tctgcgccgc
      241 gaaccggtct cgcgcatcct cggcaagaaa gggttctgga agatcatgct gaccgtcacc
      301 cccgacgtgc tcagcccccg cccggacacc gaggccattc tcgacgtcgt catgctggcc
      361 tatccgccca accgcgcctt cgagatgatc gatctgggca ccggctccgg cgccatcctg
      421 ctggccaccc tggccgagcg ggccggcgcg cgcggcgtcg gcaccgacat ttcctccgag
      481 gcgctggccg tcgcgaagga gaacgctgcc aacctcgagc tcgacaaccg cgcgaccttc
      541 ctgcgcaccg aatgggccgc gggcttcggc gacgacagct tcgatctggt cgtctccaac
      601 cccccctaca tcccctcggg cgacatcccc ggcctcgatc ccgaggtccg cgaccacgac
      661 cccctgctgg cgctggacgg cggccccgac ggtctggtcg cctaccgcga actggccccc
      721 gagatccgcc gcatcctgcg tcccggcggc gtcttcgccg tcgagatcgg ctgggaccag
      781 ggcccgcagg tcaaggccct gttcgaggcc gccggtttcg aaggggtcat cgtcgtcaag
      841 gacctgtccg atcgcgatcg ggtggtgacc aacggaccgg atccgcgcac caatcccatt
      901 ccgaagatgt agacggccga aaacttcccc ttggaaaccg gctccgcccg cgctaagtct
      961 tcgtctgtct cccacccaaa acgcacgcct tggtccgctt ctggcccatc cacgcgcgct
     1021 cagggcagtg acggtcgggc caggtcgccc ggcggaccac ggggcgacaa cggatttccg
     1081 atcacatcgt cagcggggat tggcctcgcg cgaagcggaa cgacgatacg gacggcgaca
     1141 ggctgcacct cgcaggcccg cccgtccctc atacaaagca cggtagcctc cgatgagaga
     1201 tttcaagggc atgaagcgtc agcgcggacg caacaggaag cccggcagcg gcggcggaaa
     1261 caacaacgcc aacgccacca atccgaaccg ttcgtgggac tcgcaaggcc ccgagaacat
     1321 caaggtccgc ggcaacgccc agacggtcta cgagcgctac atgcagctgg cgcgcgacgc
     1381 ctcggccgcc ggcgaccgcg tcctggccga gaactacacc cagcacgccg aacactattt
     1441 ccgcgtcctg cgcgccctgc agccctcgcg tcccgtcagt gaaatcgccc agcgcgaact
     1501 ggccggtcag ggcttcgaca tcgatttcga ggacgagacc ggcgcccagg ccgccgccat
     1561 catggccgcc gagaaggtcg aggccgaccg tctcgcccag atcgaggccc gccagcgcga
     1621 gaacgaagag cgctccaacg atcgccaggg tgatcgccag ggcggcgaac agccccgccg
     1681 cgaatggcgc gaccgcgacg accagccgcg cgccgaccgc gagccgcgcg aaccccgcga
     1741 accgcgtccc gagggcgagg cctctaccaa ccccgacggc agccgccgcg agacccgccg
     1801 cgagcgctgg gagcgccgtc agctggaacg caacgcccgc ttcgaccgcg aaggcggcga
     1861 acgcccggag ggcgacgctc cgccttccga cgaagcgccc gaagcccgcg ctgaaaccgt
     1921 tgagcgtccc gaacgcccgg aacgcgaaga gcggctggcg cgcgccgaac gccccgagcg
     1981 ccgcccccgc cgcgacgccg gcgtggccgc cgacgacggg ccttcggccc tgcccggctt
     2041 cctgacccgt tcggccgccc cggcccccgt cgaggccccc gccgccgccg agacgtccga
     2101 agaagccccc gcgcccaagc gtcgcgcccc gcgccgcaag gtcgaggctc cgaccgagga
     2161 ctgagacctg tcccgcggcg ccggcgcgct tgacgccgcc gccgcggact ggcccaagga
     2221 gcgtccgacc ccaagcggag cgcccatgac ccgactgtcg cgatccccgg ccctgatcgc
     2281 ccttgcggcg ctggccctga cggcctgcgc cagcgccccg ccgcccccgc cgccgccttc
     2341 cggaaccggc gccgccctgc gcgactggcg cggcatcgtc acctcggtcg atcgcgaccg
     2401 ctacgcccgc cgcgacgccg cctggtcgct ggccctgcag caggcgcgcc gccagaccgg
     2461 ctcgggcgac ctgcgcgcgc tcggcgacct gatcgacccc gacattcgcc gcgcgcccgt
     2521 ggccccgccc ccgggcgcct accgctgccg caccgtcaaa ctgggctcgc agggcggcga
     2581 acagggcctc ggctatgtcg tctacggctg gttcgcctgc cgcatcgagc agacgccgcg
     2641 cggcctgaaa ttctccaaac tgaccggcag ccagcgccct tcgggcctgc tgttccccga
     2701 gaacgaccgc cagatggtca tgctcggctc gctggccctc gccgaggagc ccgccgccaa
     2761 ctcctatggc cagcgccccg accgcgacct gatcgccgtg ctggagcgga tcggcgaccg
     2821 ccgctggcgg ctggtcctgc cctggccgca ggcggaatcg aacctcgacg tgatcgaact
     2881 cgtccccgcg tgacgaagac gccggccccg aaaccccgca agccgcgggc caaggccgcg
     2941 tcgggtcggg aggtcccgaa gggccgacga ccgcggccca caagaaaggc gcccgaagcc
     3001 ctcgacatcc cgcccccgcc gcgccgggcc cgccccgccg acatcgccgt gatcggcgcc
     3061 ggccccaccg gtctggccgt cgccctgatg ctggtccggc agggccgccg cgtcatggtt
     3121 cacgagcggt tcgccaggcc tcaccccatc ggcgccggct tcctgcttca gcccaccggc
     3181 ctgcacgtgc tcgacggcct cgggctgacc ccgcaggtcg aggcggtcgg ccagcgcatc
     3241 gaccacatct tcggccgcga ggcccgtcgc ggccgcgtcg tgctggatat ccgctacagc
     3301 gatctgaaac gcccgcgtca ggccatcggc atccaccgcg ccgccatgtt cgacatcctc
     3361 tacagcgcct gcgtcgaggc tggcgtcgac ttcgaaaccg accgcgagat cgtcgccgcc
     3421 gatgcgggcc atttgatcga cagcggcggc cgccgcagcg cgaccccgtt caatctcgtc
     3481 atcgacgcct cgggctcccg ctccgccatc gccgccggca tgggcgagga gcggcgcgac
     3541 ctcgcctggg gcgcgctgtg gaccaccgtc ccctggcccg gcgccccctt cgacccgacg
     3601 gcgctggaac aggtctatct gggcgccagc cgcatgctgg gcgtcctgcc ctgcggccgc
     3661 cttcccgacg atccggcgcc gctggccacc ttcttctgga gcctgaagac cgagatctgg
     3721 gccgactggc acacgcacgg cctcgacgcc tggaaggcgc actgcctgcg cctctggccc
     3781 gaactgatgc cggtcatgga cgccatcacc gaccgtgagc agatgaccct ggcctcctac
     3841 ggccaccaga ccctcgcccg ccccgtcggc gaccggctgg ctatagtggg cgacgccgcc
     3901 cattcgacca gcccccagct cgggcagggc gtgaacatgg gactgctgga cgcgctggcc
     3961 ctgaccgggg ccctcaggca gcaccgcgac ctgccgaccg ccctgaacgc ctacgcccgc
     4021 gcccgccgct ggcatgtgcg actgtatcag gccctcagcc tcggcttcac ccccttctat
     4081 caggccgacg gccgccttct gccctggttc cgcgaccatg tgctgggcaa ggtcgcccgc
     4141 ctgccgctgg ccccgcgcct gctggccgcc acggtcgccg gcgtcctgct ggacccgcgg
     4201 tcgtccggaa gtggctgaac cccttcaggc cacagccgtt tgcacggttc a
//
LOCUS       DASVVN010000174         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_164849,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000174 DASVVN010000000
VERSION     DASVVN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164849"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF686_02680"
     CDS             <1..375
                     /locus_tag="VF686_02680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8660746.1"
                     /translation="TEAHGPHRVTLNGLPVGLGPAAALSMGMIFHELATNAAKYGALS
                     APEGRVLVDWSISNQTHPVLHLTWRELGGPPVAAPERRGFGSRLIERNVRHDLAGEIK
                     LDYASNGFSADFSIPLHRGTQS"
     gene            372..749
                     /locus_tag="VF686_02685"
     CDS             372..749
                     /locus_tag="VF686_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8660747.1"
                     /translation="MSAPLTGRRVLVVEDESLVAMLLETILEDMGCTPVGPASNIDDG
                     LKMATDGEHLDAALLDVNVAGRQVFPVAEALKARGVPFVFSTGYGEGGLPDEWRGQAT
                     IQKPFTEAAVRDALMKAMGVEQA"
     gene            complement(886..>1591)
                     /locus_tag="VF686_02690"
     CDS             complement(886..>1591)
                     /locus_tag="VF686_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8660748.1"
                     /translation="ARAVVSNLHPRLLFERFVDDAVVPADFRERIGNYQSGSGTFRMN
                     VALSELPNFTALPGQGDHLTAGIIIAPSLGYMDRAHAEARLNGWSGKPIVEMLIPSTL
                     DDSLAPPGQHVASLFCQHVSPDLADENRDTVADLMIETVDAHAPGFKASVVGRLALGP
                     RDLERRFGLVGGDIFHGRLSLDQLFSARPVLGHADHRMPVPGLYLCGSGAHPGGGVTG
                     APGHNAAKALLKDLGR"
BASE COUNT          275 a          570 c          509 g          237 t
ORIGIN      
        1 accgaggcac atggcccgca tcgcgtcacc ctgaacggcc tcccggtcgg actggggccc
       61 gcggccgccc tgtccatggg gatgatcttc cacgaactgg cgaccaacgc cgccaaatac
      121 ggagccctgt ccgcgccgga aggacgggtg ctggtcgact ggtccatttc gaaccagaca
      181 cacccggttc tgcacctgac ctggcgcgag ctcggcggtc cgcccgtcgc cgcgcccgaa
      241 cgccgcggct tcggcagccg gctgatcgaa cgaaacgtgc gccacgatct ggcgggcgag
      301 atcaaactgg actacgcaag caacggtttc agtgcggact tttcgattcc gctccatcgc
      361 gggacacaat catgagcgca ccccttaccg gccgacgggt tctcgtggtc gaggacgaat
      421 ccctcgtcgc catgctgctc gagaccattc ttgaagacat gggctgcacg cccgtcggac
      481 cggcgtccaa catcgacgac ggcctgaaga tggcgaccga cggcgaacac ctcgacgccg
      541 ccttgctgga cgtcaacgtc gccggccgtc aggtcttccc cgtcgccgag gcgctgaagg
      601 cgcgcggcgt gccgttcgtc ttctcgaccg gctatggcga gggcggcctg ccggacgaat
      661 ggcgcggtca ggccacgatc cagaaaccct tcaccgaggc cgccgtgcgc gacgccctga
      721 tgaaggcgat gggcgtcgaa caggcctgaa tttcaccgtg catcccggcc aatgccggga
      781 cccagatgtc atgacaccaa ctccaaccgt cgcgcgcgcc tctcatcacc agccttcgag
      841 cttgtgatct ggatcccggc attcgccggg atgcacggaa gggggttacc gccccagatc
      901 cttcagaagg gccttagccg cgttgtggcc cggggcgcct gtgacgccgc cgcccggatg
      961 ggcgcctgat ccgcacagat agaggccggg caccggcatc cggtggtcgg cgtggcccag
     1021 caccggccgg gcgctgaaca gctggtccag cgacagccgg ccgtggaaga tgtcccctcc
     1081 gaccagcccg aagcgccgct ccaggtcgcg cggccccagc gccagccgcc cgaccaccga
     1141 ggccttgaag cccggcgcat gggcgtcgac cgtctcgatc atcagatcgg cgaccgtatc
     1201 gcggttctcg tcggccagat cgggcgagac atgctggcaa aacaggctgg ccacatgctg
     1261 tcccggcggg gccagactgt cgtcgagcgt cgaagggatc agcatctcga cgatcggctt
     1321 gcccgaccag ccgttcagcc gcgcctcggc atgcgcccgg tccatatagc ccagcgacgg
     1381 ggcgatgatg atgcccgccg tcagatggtc gccctgcccc ggcagggcgg tgaagttcgg
     1441 cagctccgac agcgccacat tcatccggaa ggtgcccgag ccggactggt aattgccgat
     1501 ccgctcgcgg aagtccgccg gcacgaccgc atcgtccacg aaccgctcga acagcagccg
     1561 cggatgcaga ttggacacca ccgctcgcgc c
//
LOCUS       DASVVN010000175         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_400958,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000175 DASVVN010000000
VERSION     DASVVN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400958"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VF686_02695"
     CDS             <1..628
                     /locus_tag="VF686_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660749.1"
                     /translation="ADVAVLPRWMRVSQVLDYTAGVHPGFDRAKAKDFLARTTIGLDR
                     KVRELSKGMVTQLHLALVMSVDARLLVLDEPTLGLDLLYRKKFFDNLLSDYGDHDRTI
                     VISTHDIQEIQHVLTDVVFLDRGRVALSCTMDAIEARFVEATVSADTLAAARALKPIH
                     ERQTFGRTLVMFDGLERDQLAAFGDLRTPSLPDLFVALLGDEPMRAAA"
     gene            625..1509
                     /locus_tag="VF686_02700"
     CDS             625..1509
                     /locus_tag="VF686_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660750.1"
                     /translation="MTDLPKTPWSRTLYWQIRREFWEHRAIWIAPLAVAAFIVVIHVL
                     SALVIPGAQRAAVLADPVKAREFLFPYSAVIGATMVTGMVVGLLYSLEALQGERRERS
                     ILFWKSLPVSDRMTVLSKATVPVVLMPLIVVGIVAAATAVMLTLQSLIWGLGGFSPSV
                     LWARIDLPLMWLSLLYGLPFMVLWNAPLFAWALLVSAWSRRMPILWAAAPFVAVLLVE
                     HTVLHHTPAHWIVERRVGGAILQPYSLEGTGVQWVQRLSDLDPVRLYALPGLWIGVVL
                     AAALLAAAIRVRRSRTPY"
     gene            1657..2346
                     /locus_tag="VF686_02705"
     CDS             1657..2346
                     /locus_tag="VF686_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660751.1"
                     /translation="MTLPAASQDGFSVLQAILIVHVSGGGVGLVAGFLAVAAPKGGVL
                     HRRAGTVFVVAMVTMAAFGLAVAASRGQPLNILAAAFTLYLVASAWLTVRSRRGEAGW
                     AERFGCLYASGVAAFAAAVATGAGAGEPEPAASVFGAVAALAAVLDLRVILRRGVSGV
                     SRLSRHLWRMCAALFVASGSFFLGQMDEIPAALRGPHLFVLAFAPLVALVFWLVRVRF
                     TRSAKSVPVAP"
     gene            2390..>3532
                     /locus_tag="VF686_02710"
     CDS             2390..>3532
                     /locus_tag="VF686_02710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX8660752.1"
                     /translation="MRNVLMGIAAVAALLCAQPAAADTVSDWWEFANKIAPQGAPGST
                     PEQGRAVTQASLAMFEALNAIDRRYESYLDFPAADPRASQDAAAVTAAYTVMLHHYPA
                     QKAALDEGYAIAMGAIPDEGARDSGRQIGERAAAAAIAAGGIDAVMTTVPYRPRTTPG
                     VWVSTQLPQIEPYMSTYKPWAIPSAEALRPPPPPALNSERWARDYEEVRRLGGRTSAD
                     RTPQQTLMARYRQAFDLTPTMRQIADGPGRRPVQNARMFALYQMAFDDAALAMVAAKA
                     HYDFWRPITAIRNGGEDGNDATQAEAGWTPLLGTPNFAEYPCGHCTVAAVYAAVMKTE
                     TGPRPAGGVRVGAMAVPVSAVQVLPTWDEWAQQVSDSRIYGGVHYRF"
BASE COUNT          502 a         1238 c         1148 g          644 t
ORIGIN      
        1 cgcagacgtg gcggtgctcc cccgctggat gcgcgtcagc caagtgctgg actatacggc
       61 aggggtgcat cccggtttcg accgggccaa ggcgaaagac tttctagccc gcaccaccat
      121 cggcctcgac cgcaaggtgc gcgagctttc gaaggggatg gtcacccagc tgcacttggc
      181 ccttgtgatg tctgttgacg cgcgcctgct tgttctggac gagccgacac ttgggctgga
      241 ccttctgtac cggaagaaat tcttcgacaa tttgttgagc gactacggcg atcacgaccg
      301 caccatcgtc atctcgaccc acgacattca ggaaatccag catgttctga ccgacgtggt
      361 gtttctcgac cggggccgtg tcgcgctgag ctgcaccatg gacgcgatcg aggcgcgctt
      421 tgtcgaggcg acggtctcgg ccgacaccct agccgcggcc cgggcgctga agccgatcca
      481 tgaacgtcag acgttcggac gcaccctcgt catgttcgac ggcctagagc gggaccaact
      541 cgccgcattc ggcgacctgc gcacgcccag cctgccggat ctcttcgtcg ccttgcttgg
      601 cgatgaaccg atgagggcgg cggcatgacc gaccttccca agacgccgtg gtcgcgcacg
      661 ctgtactggc agatccgccg cgaattttgg gagcatcgcg cgatctggat cgccccgctg
      721 gccgtcgccg ccttcattgt ggtgatccat gtgctttccg ccctagtcat ccctggcgcc
      781 cagcgcgccg ccgtcctcgc ggacccggtc aaggcccggg agttcctgtt cccgtacagc
      841 gccgtgatcg gcgcgacgat ggtgaccggc atggtggtcg gcctcctcta cagcctcgag
      901 gcgctgcagg gcgaacgccg ggagcgcagc atcctgttct ggaagtcgct gccggtgtcg
      961 gaccgtatga ccgtgctctc caaggccacc gtcccggttg tgctcatgcc cctgatcgtc
     1021 gtggggattg tagcagcggc caccgccgtc atgctgacgc tccagtcgct gatctggggt
     1081 ctcggcggct tcagtcccag cgtcctctgg gcgcggatcg atctgccgtt gatgtggctc
     1141 agcctgctct atggtctgcc gttcatggtg ctgtggaacg ccccgctctt cgcatgggcc
     1201 ctgctggtct cggcctggtc gcgccgaatg cccatcctct gggctgccgc gccgttcgtt
     1261 gcagttctcc tggtagaaca caccgtcctg caccatacgc ccgcgcactg gatcgtggag
     1321 cggcgtgtcg gcggtgcgat tcttcagccc tactcgctcg agggcaccgg cgttcaatgg
     1381 gtccagcgac tgtccgatct cgatcctgtg cggttgtacg ccctgccagg cctgtggatc
     1441 ggcgtggtgc tcgccgccgc cctgctcgcg gccgccatac gggtgcgccg ctcccgaacc
     1501 ccctactgat cgccgatccc ggaaaggatc cgtccatgtt gcgatacgcc gacccggccg
     1561 acactggcta gcccgcgggg cgaatagccc ccgcttcgac ccctcaccgc cgaccttttt
     1621 ccacccactt ttcgcacccc gcgaacggag gacgccatga ccttgcctgc cgccagccaa
     1681 gacggatttt cagtgcttca agccatactg atcgtgcacg tctcgggcgg cggcgtcggg
     1741 ctcgtcgcgg gttttctggc ggtcgcggcg cccaagggcg gggtgctgca ccgccgggcc
     1801 ggaaccgtat tcgtcgtcgc catggtgacc atggccgcgt ttggcctcgc cgttgcggcg
     1861 tcccgcggcc agccgctgaa cattctcgcc gccgccttca ccctctatct ggtggcgagc
     1921 gcctggctga ccgtgaggag tcggcgcggc gaggccggct gggccgagcg cttcggctgc
     1981 ctctatgcct cgggcgtggc ggcctttgcg gccgccgtcg ccacgggcgc cggcgcaggc
     2041 gagccggaac cagcggcctc ggtgttcggc gcggtggccg cgctcgccgc cgttctcgac
     2101 ctcagggtga tcctgaggcg gggcgtcagc ggcgtttcgc gcctctcccg tcacctctgg
     2161 cgcatgtgcg ccgccctgtt tgtcgcctcg ggctccttct tcctcggcca gatggacgag
     2221 atcccggccg ccctgcgtgg accgcatctc ttcgtcctgg ccttcgcgcc cctggtcgcc
     2281 ttggtcttct ggctagttcg ggttcgcttc acccgctccg ccaaaagcgt ccccgtcgcg
     2341 ccatgacgcc gtcggggcgg aacattcaca ttatcaatgg aggaacgaaa tgcgtaacgt
     2401 cctgatgggc atcgccgcgg tcgcggccct gctatgcgcc cagcccgcag cggctgacac
     2461 ggtgtcggac tggtgggagt tcgccaacaa gatcgcaccc caaggcgcgc cggggagcac
     2521 gcccgaacaa ggacgagccg tcacccaggc gtcgctggcg atgttcgagg ccttgaacgc
     2581 catcgaccgc cgctacgaaa gctacctgga ctttccggcg gccgacccgc gggcgtctca
     2641 ggacgcagcg gcggtaaccg ccgcctacac cgtcatgctt caccactacc ccgcgcagaa
     2701 ggcggctctg gacgaaggct atgctatcgc tatgggcgct atcccggacg agggcgcgcg
     2761 ggactccggc cggcagattg gggaacgggc cgcggccgcc gccatcgcgg cgggcgggat
     2821 cgacgccgtc atgacgaccg ttccgtatcg tccgcgcact accccaggcg tgtgggtgtc
     2881 gacccaactg cctcagatcg agccctatat gtcgacctac aagccttggg cgatccccag
     2941 cgccgaagcg ctgcggcctc cgcctccgcc ggctctgaat agcgaacggt gggcgcgcga
     3001 ctacgaggag gtcaggcggc tgggcgggcg aacgagcgcc gatcggacgc cgcaacagac
     3061 tttgatggcc cggtaccgcc aagccttcga tctgaccccg accatgcggc agatcgccga
     3121 cggccctggc cgtcgcccgg tacagaacgc gcgcatgttc gcgctctacc agatggcgtt
     3181 cgacgacgcc gccctcgcga tggttgcggc caaggcgcac tacgacttct ggcgccccat
     3241 caccgcgatc cggaacggcg gcgaggatgg caatgacgcg acgcaagccg aggcgggctg
     3301 gaccccgctt ctgggtacgc cgaactttgc cgagtatccc tgcggccact gcaccgtcgc
     3361 ggcggtctac gccgcggtca tgaagaccga gacgggtccg cgtccggccg gcggtgtcag
     3421 ggttggggcg atggccgtgc ccgtatcggc ggtccaggtc cttccgacct gggacgagtg
     3481 ggcgcagcag gtttcggact cgcggatcta cggcggcgtc cactaccggt tt
//
LOCUS       DASVVN010000176         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_453442,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000176 DASVVN010000000
VERSION     DASVVN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453442"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF686_02715"
     CDS             complement(<1..458)
                     /locus_tag="VF686_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX8660753.1"
                     /translation="MLGIALSGFFDGILLHQVLQWHHLLSLVPGETIQRLTTQILADG
                     LFHVLMYVIALGGGIWLWRQREGLAAPGGSRRLAVGLLAGFGAWNVVDVVLFHWILEI
                     HHIRLDTSQPLAWDAGWLVMLGLVPLALAGLCSGRSASGGGSGGGRGTAVA"
     gene            complement(674..1231)
                     /locus_tag="VF686_02720"
     CDS             complement(674..1231)
                     /locus_tag="VF686_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX8660754.1"
                     /translation="MTNKTGLEFKVGDAVVYPAHGVGKVAAVETQEVAGMSLEVYVVT
                     FDHEKMTLRVPTKKAKTAGLRSLAADDVVTKALTTLKGRARVKRTMWSRRAQEYEAKI
                     NSGDLISIAEVVRDLHRADSQPEQSYSERQLYESALDRMAREVAAANRIDKDAAVQLL
                     AKSLSAKKAVIAAAEAAEEAEAEAA"
BASE COUNT          271 a          503 c          481 g          248 t
ORIGIN      
        1 gcaactgccg tcccgcgacc cccgccgctt ccgcctccac tggcggatcg ccctgagcac
       61 aggcccgcca gcgccagagg aacgaggccc agcatcacca gccacccggc gtcccacgcc
      121 agcggttgag acgtgtccag acggatgtgg tggatctcca gtatccagtg aaaaagcacg
      181 acgtcgacca cgttccacgc gccgaacccg gccagaagtc cgaccgcgag ccgccgcgat
      241 ccgccgggcg ccgccagccc ttccctttgg cgccacagcc agatcccgcc gccgagggcg
      301 atcacgtaca tcaggacatg gaacagcccg tcggcgagga tctgcgtggt cagccgctgg
      361 atggtttcgc cgggcacgag gctgagcagg tgatgccact gcaggacctg atgcagcagg
      421 atgccgtcaa aaaagccgct cagggcgatg cccagcacga aggcggaacc gaagggggcg
      481 tgtctggaaa cctggcgcat cagaacctcg ctcgcacagg cacaaggctg cagggcgggc
      541 atcgttcctg tgtccatgac ccgacagccc ggcgccgacg ggtcgagcgc cagcgttggt
      601 gtcctcaccg gctcaaagaa aaaggcccgg tgtttccacc gggcctctcg tcattcagcg
      661 atgcgcgtcg aaatcaggcg gcttcggcct cggcctcttc cgcggcttcg gcggcggcga
      721 tgacggcctt cttggcgctc agcgacttgg ccagcagctg gacggccgcg tccttgtcga
      781 tgcggttggc ggcggcgact tcacgggcca tccggtccag ggccgattca tagagctgac
      841 gctccgaata ggactgttcc ggctggctgt cggcgcggtg caggtcgcgg accacttcgg
      901 cgatcgagat cagatcgccc gagttgatct tggcttcgta ttcctgggcg cggcgcgacc
      961 acatggtgcg cttgacgcgg gcgcggccct tgagggtcgt cagcgccttc gtgacgacgt
     1021 cgtcggcggc cagcgaacgc aggccggcgg tttttgcctt cttggtcggg acgcgcaggg
     1081 tcatcttctc gtggtcgaag gtcacgacat agacctccag cgacatgccc gcgacttcct
     1141 gggtctcgac cgccgccacc ttgccgacgc cgtgcgccgg atagacgacc gcgtcgccaa
     1201 ccttgaattc cagaccagtc ttgttcgtca tgtcattcct ttcccggcgc agggaggatg
     1261 agacggttac gcaagtgacg gctgcgctct tcggcgacgg cgaaccgtcg acggcgcagc
     1321 tatattcagt cgtcaggagc gaaaacggat caccaaacct ctggccgacc ccgccttcgt
     1381 gggcggagtt ctgtcgcaaa cgcgggcggc gcgagaaaat cgcttaccgc ccgacccaat
     1441 gacaggaacc tatcacaaaa ttgcggaatt tcaaaacgtc cacagcgtca gcgcgcggaa
     1501 ggg
//
LOCUS       DASVVN010000177         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_479182,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000177 DASVVN010000000
VERSION     DASVVN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479182"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1686
                     /locus_tag="VF686_02725"
     CDS             16..>1686
                     /locus_tag="VF686_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8660755.1"
                     /translation="MHLTTNATGPDNLASNADVVSGAQRGTNGLSSANLRGQGNAATL
                     VLLNSRRIAAHGLSGSAVDVNQIPFSAIDRIEVLKDGASAIYGTDAVGGVINFITRKD
                     YQGLGLQGFTDATQRGDGNIYRLSAIAGYGDLNEQGFNVMGAVSYSWNQILRGSDRSF
                     VNGNQPNRGLSIDTRGAPIATVFNTAGNIHQTPAGTLLTGLTLTLPNGANGAGGGINI
                     LDLPGGAGCDSVDGGLPYDEVLWNTATAQYACSWDTGRAAVIQQPIQTLTYYGRGVLR
                     LGGSHEVSLEVTGSDADSSKSFSANQYTASATSMPWAYPLNPLTKATYDQVFNSIRAA
                     FTNPAQQAALDARYGRPIAGRWRCIACGPREYETNTKTFRAGLGIDGPLWEGWDYRAG
                     ASYARSESSSVLGTGYHYRGVFVTNDPSTPINDIGAYDTRAPTAPGAAGPGIVGLINA
                     GILNPFSVVQTPQALAGLEAVSAEGTTLYGGRYEVKQFDASLAGRLFEIWGGDIQLAV
                     GVDYRRETYRFNGSAAAAADQPDIFNVAFDNTNALAPQNRTVKAAYAEL"
BASE COUNT          290 a          606 c          518 g          272 t
ORIGIN      
        1 cccgagcagt tgctgatgca tctcaccacc aacgccaccg gccccgacaa tctcgcgtcc
       61 aacgccgatg tcgtctcggg cgcccagcgg ggcaccaacg gcctttcctc ggccaacctg
      121 cgcggccagg gcaatgccgc caccctggtc ctgctcaaca gccggaggat cgcggcccac
      181 ggcctcagcg gttccgccgt cgacgtcaac cagatcccct tttccgcgat cgacaggatc
      241 gaggttctca aggacggcgc ctcggccatt tacggtaccg acgcggtcgg cggcgtgatc
      301 aacttcatca cgcgcaagga ctatcagggc ctcggcctgc agggctttac cgacgccacg
      361 cagcgcggcg atggcaatat ttaccgtctc tcggccatcg ccggctatgg cgatctgaac
      421 gagcagggct tcaacgtgat gggcgcggtc tcctatagct ggaaccagat cctgcgaggc
      481 tccgatcgca gcttcgtcaa cggcaaccag cccaataggg gcctttcgat cgacacgcgc
      541 ggcgcgccga tcgcgaccgt gttcaacacc gccggcaata tccaccagac gccggcggga
      601 acgctgctca ccggccttac cctcacgctg ccgaacggtg ccaacggggc tggcggcggc
      661 atcaacatcc tggacctgcc cggcggcgcc ggctgcgact cggtcgacgg cgggctgccc
      721 tatgacgagg tgctgtggaa caccgcgacc gcgcaatatg cctgctcgtg ggatactggc
      781 cgcgcggcgg tgatccagca gccgatccag acgctcacct attacggccg cggcgtcctc
      841 cgccttggcg gctcgcatga ggtgtcgctc gaagtcaccg gctccgacgc ggattcctcc
      901 aagagcttct ccgccaatca gtacaccgcc agcgccacct ccatgccctg ggcctatccg
      961 ctcaatccgc tgacaaaggc gacgtacgac caggtgttca attcgatccg ggctgcgttc
     1021 accaacccgg cgcagcaagc ggccctcgat gcgcgctacg gccggccgat cgcgggccgc
     1081 tggcggtgca tcgcgtgcgg gccgcgcgaa tacgagacca acacgaagac gttccgcgcc
     1141 gggctcggca tcgacggccc gctttgggaa ggctgggatt accgcgcggg cgcttcctac
     1201 gcgaggagtg aatcctcctc agtgctgggg acaggctatc actatcgcgg cgtgttcgta
     1261 acgaacgatc cttcgacgcc gatcaacgac atcggcgcct acgacactcg agcgcctacc
     1321 gcgcccggcg ccgccggccc cggcatcgtc ggcctgatca acgctggcat cctcaacccg
     1381 ttctccgtcg tgcagacacc gcaggcgctg gccgggctcg aagcggtgtc cgccgagggc
     1441 acgacgctct atggcggccg ctatgaggtg aagcaattcg acgcttcgct tgcgggccgg
     1501 ctgttcgaga tctggggcgg cgacatccag ctcgccgtcg gcgtggacta tcgccgcgag
     1561 acctataggt tcaacggctc ggcggctgcc gcggccgacc agcccgacat cttcaacgtg
     1621 gccttcgaca acaccaacgc gctggcgcct cagaaccgca ctgtgaaggc ggcttatgcc
     1681 gagctg
//
LOCUS       DASVVN010000178         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_164980,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000178 DASVVN010000000
VERSION     DASVVN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164980"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VF686_02730"
     CDS             complement(<1..1541)
                     /locus_tag="VF686_02730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8660756.1"
                     /translation="MSFTACRAALTGASLLALAACATAPSTATPLSTEPAAIVTPTRE
                     STAFTLGTDQPRTPEQLAMAFDKADLSIKVIPADKAIDAVAVLDFTATAPLSAVVVEL
                     DTLLTVSSVQVDGREVAAGDWTNPEGRMTVPLPRPLAAGEKVSVRIAYAGKPRVAPHA
                     PWDGGFVWANTADGTPWIATAVQPEGCDIFWPCIDSPLAEPGRVDLHITVPSNLSAPS
                     NGRFLGTVDHGDGWTTWNWSAAHPNTYAIALNIGPYDEVKADYRSRFGNVIPLSYWHL
                     RSDDPVKVQALFAQFAPMMDFYEATVGPYPFGDEKMGVVETPHLGMEHQTINAYGNEY
                     KLDGRGFDWLLHHELSHEWFGNQLTNRNADDMWLHEGFGSYMQPLYDRWLNGDRFMQA
                     SLHEQRRDLINKFPVVSGTLKSEDEVYKGDVGPGLDIYNKGSLIAHSLRMLIGDEAFF
                     RSVTRIVYGTAEPRPGQFEPRYGTTDELLAIVNEETGRDLTWFFRGYLYNAALPDLKQ
                     TRDGD"
BASE COUNT          262 a          538 c          647 g          290 t
ORIGIN      
        1 tgatcgccgt cgcgggtctg tttcaaatcc ggcagggcgg cgttgtagag gtagccgcgg
       61 aagaaccagg tcaggtcgcg gcccgtctcc tcattgacga tggccagcag ttcgtcggtc
      121 gtgccgtagc gcggctcgaa ctggccgggg cggggctcgg ccgtgccgta gacgatgcgc
      181 gtcaccgagc ggaagaaggc ctcgtcgccg atcagcatgc gcagcgaatg ggcgatcagc
      241 gagcccttgt tgtagatgtc gagacccggg ccgacgtcgc ccttgtagac ctcgtcctcg
      301 gacttgagcg tgccggagac gacggggaat ttgttgatca ggtcccggcg ctgctcgtgc
      361 aggctggcct gcatgaagcg gtcgccgttc agccagcggt cgtagagggg ctgcatgtag
      421 gagccgaagc cctcgtgcag ccacatgtcg tcggcgttgc ggttggtcag ctggttgccg
      481 aaccattcgt ggctcagttc gtggtgcagc agccagtcga agccgcggcc gtccaacttg
      541 tactcgttgc cgtaggcgtt gatcgtctgg tgctccatgc ccaggtgcgg cgtctcgacg
      601 acgcccatct tctcgtcgcc gaaggggtag gggccgacgg tcgcctcgta gaaatccatc
      661 atcggcgcga actgggcgaa cagggcctgg accttgaccg gatcgtcgct gcgcagatgc
      721 cagtaggaca gggggatcac attgccgaac cggctacggt agtcggcctt gacctcgtca
      781 tacggcccga tgttcagggc gatggcgtag gtgttgggat gggccgcgct ccagttccag
      841 gtcgtccagc cgtcgccgtg atcgaccgtg ccgaggaaac ggccgttcga cggcgccgac
      901 aggttcgacg gcacggtgat gtgcagatcc acccgcccgg gctcggccag cgggctgtcg
      961 atacagggcc agaagatgtc gcagccctcg ggctggacgg cggtggcgat ccagggcgtg
     1021 ccgtccgccg tattggccca gacgaagccg ccatcccaag gcgcgtgcgg cgccacgcgg
     1081 ggcttgccgg cgtaggcgat ccgcacagag accttttcgc cggcggccag cggccggggc
     1141 agggggacgg tcatccggcc ttcgggattg gtccagtcgc ccgccgcgac ctcgcggccg
     1201 tcgacctgca ccgacgagac cgtcagcagg gtgtccagct cgaccaccac ggcgctcagc
     1261 ggcgcggtgg cggtgaagtc cagcacggcg acggcgtcga tcgccttgtc ggcggggatg
     1321 accttgatcg acaggtcggc cttgtcgaag gccatggcca gctgttcggg ggtgcgcggc
     1381 tggtcggtgc cgagggtgaa ggctgtggat tcgcgcgttg gcgtgacgat ggcggcgggc
     1441 tcggtcgaga ggggagtagc ggtcgagggc gccgtggcgc aggcggcgag agcgagcagg
     1501 gaagcgccgg tcagtgcggc gcggcaggcg gtgaaggaca cgaacggctc cccgaaaact
     1561 gacagccagc ctagacaggc gggcggctcg tgcaagcctt acaagccccg cgccttgacc
     1621 tgcgccttcg tcgcccgccg ggcccaggcc ttgtcgatct gttcgcgcag ccggtcgtcg
     1681 tcggccgcct cggagcggac cagcacggcg ggccagcctt catagtgtgg cgtctgg
//
LOCUS       DASVVN010000179         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_348171,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000179 DASVVN010000000
VERSION     DASVVN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348171"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF686_02735"
     CDS             <1..321
                     /locus_tag="VF686_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660757.1"
                     /translation="RGKEGHRQLRGEGLMRILVLAVAATVLSGCVTSYSGDAVTRFVE
                     RAEMCVHWSGEEPYDEARRAEIDRALRDMRCATLLRDSAALKVSRKDRPEDVRRIDEA
                     LSAF"
     gene            334..1656
                     /locus_tag="VF686_02740"
     CDS             334..1656
                     /locus_tag="VF686_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8660758.1"
                     /translation="MITRRAAIATSLAASAATLAIGGPAVARPAPPAGFVRREGTALT
                     LDGRPYRFVGSNMWYAAWIAGQPRLGRELDALAGLGVTNLRVAASAELSPLKNSVRPA
                     FRDETGAYDERLLTGLDQAMAEIRRRGMKAVLYLTNFWEWSGGMATYLLWTNGGRYID
                     MNDPAHPWPAFPDFVAGFYRSPEAVALYHDHVRRIVGRMNSVTGVAYAEDPTIMAWQL
                     ANEPRPGGSAAVADETLADFHGWIGSTARLIKSIDPNHLVSTGGEGLKGSIEREDLLL
                     SSQAVEDIDYMTAHLWPGNWSWLDRSDMAGTHGRAMTQSADYIARHVALAGRQGVPLV
                     LEEFGYPRDGDRYGLDVPTTMRDEFYGMVHGAVLTSAREGGPLVGSNFWAWNGEGRAA
                     HTDARFRIGDDALLGDPPHEPQGWYGVFDTDHSTRALVRAQAAALAAL"
     gene            complement(1662..>2835)
                     /locus_tag="VF686_02745"
     CDS             complement(1662..>2835)
                     /locus_tag="VF686_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8660759.1"
                     /translation="GDLLRTRDGGHARVGFVELFFDLIFVFAITQISHALLAHLSWIG
                     ALQGFMLLGAVWWAWIDTSWITNWLDPDRAPVRALLFLLMGAGLVMSTSLPEAFDDKG
                     LIFACAFASLQVGRGLFALWAVRGHEVLRANFQRICIWAIAGAVLWIAGGFLEGQTRL
                     ILWLIAVAAEFAAPACYFWVPGLGRSHTEDWEVEGSHMAERVGLFIIICLGESVLVTG
                     ATFAGLEWSAPVVAAFATAFVGTLAMWWIWFSKAHDAASDVIAHARDTGRVARRAYTY
                     APILVVAGIVVTAVGDEMSLSHPGGAVDFAMAAVLIGGPMLFLIGAQVFKLAAFGIWS
                     PSRLSGIAVLGALGFAVPMLTPVGLAACATVVLVGVAAWETVATHRMPAEEAKTPE"
BASE COUNT          480 a          950 c         1004 g          401 t
ORIGIN      
        1 cggggaaaag aaggtcatcg acagcttcgt ggagaaggcc tgatgcggat actggttctc
       61 gcagtcgcgg ccaccgtcct gtcgggatgc gtcacctcgt acagcggcga tgcggtgacc
      121 cggttcgtcg agcgggccga aatgtgcgtc cactggtcgg gcgaggagcc ctacgacgag
      181 gcgcggcggg cggagatcga tagggccctg cgcgacatgc gatgcgcgac gctgctgcgc
      241 gatagcgcag cgctgaaggt cagccggaag gaccggccgg aggacgtccg acggatcgat
      301 gaggccctgt cggccttctg agtggaagca gccatgatca cgcgccgcgc cgctatcgcg
      361 acctcgctgg ccgcctcggc cgcgacactc gccatcggag ggccagccgt ggcccgcccg
      421 gcgccgccgg ccggtttcgt tcgtcgcgag ggaacggcgc taacgctgga cgggcggccc
      481 tatcggttcg tcggatcgaa catgtggtac gcggcctgga tcgccggtca gccgcggctg
      541 gggcgggagc tggatgcgtt ggcggggctg ggcgtcacca atctgcgcgt cgccgcctcg
      601 gccgagctgt cgccgctcaa gaattcggtg cggccggcgt tccgggacga aaccggcgcc
      661 tatgacgagc ggttgctgac cggcctcgat caggcgatgg cggagatccg caggcgcggg
      721 atgaaggcgg tgctgtatct caccaatttc tgggagtggt cgggcgggat ggcgacctac
      781 ctgctgtgga ccaacggcgg gcgctatatc gacatgaacg atccggcgca tccgtggccg
      841 gcgtttcccg atttcgtcgc cggcttctac cgcagtcccg aggccgtcgc cctgtatcac
      901 gaccatgtcc gtcggatcgt gggacggatg aacagtgtca ccggcgtggc ctatgccgag
      961 gatccgacca tcatggcgtg gcagctggcc aacgagccgc ggccgggcgg cagcgccgcc
     1021 gtagccgacg agaccctggc ggacttccac ggctggatcg gctcgacggc gcggctgatc
     1081 aagtcgatcg acccgaacca tcttgtctcg accggcggcg agggcctgaa gggatcgatc
     1141 gagcgcgagg atctcctgct gtcgtcgcag gcggtcgagg acatcgacta tatgacggcg
     1201 cacctgtggc cgggcaactg gagctggctc gaccggtcgg acatggcggg gacgcacggg
     1261 cgggccatga cgcagtccgc cgactacatc gcccgccacg tggcgctggc ggggcggcag
     1321 ggggtgccgc tggtgctgga ggagttcggc tatccgcggg acggagaccg ctacggcctt
     1381 gatgtgccga cgacgatgcg cgacgaattc tacggcatgg tccacggcgc ggtcctgacc
     1441 agcgcccggg aaggcggtcc gctcgtcggc tcgaacttct gggcgtggaa cggcgagggc
     1501 cgggcagcgc ataccgacgc ccgcttccgc atcggcgacg acgccctcct cggcgacccg
     1561 ccgcatgaac cgcagggatg gtacggggtg ttcgatacgg atcactccac ccgcgcgctg
     1621 gtccgggcgc aggccgcggc tctggctgcg ctttagaggc cctactccgg cgttttcgct
     1681 tcctcggcgg gcatgcgatg cgtcgccacg gtctcccagg ccgccacgcc aaccagcacg
     1741 acggtggcgc acgccgcgag gcccaccggc gtcagcatcg gcaccgcgaa gcccagcgcg
     1801 cccagcacgg cgatcccgct cagtctcgac ggcgaccaga tgccgaaggc cgccagcttg
     1861 aagacctgcg ccccgatcag aaacagcatc ggtccgccga ttagcaccgc ggccatggcg
     1921 aagtcgacgg cccctccggg gtggctcaac gacatttcgt cgcccacggc ggtgacgacg
     1981 atgccggcga cgacgaggat cggcgcatag gtgtaggccc gccgggcgac ccggccggtg
     2041 tccctggcgt gggcgatcac gtccgaggcg gcgtcgtgcg ccttgctgaa ccagatccac
     2101 cacatggcca gcgttcccac gaaggccgtg gcgaaggccg ccacgaccgg cgcggaccac
     2161 tcaagcccgg cgaaggtcgc gccggtgacg aggacggatt cgcccaggca gatgatgatg
     2221 aacaggccga cgcgctcggc catgtgactg ccctcgacct cccagtcttc agtatgtgag
     2281 cggccgaggc ccggtaccca gaaatagcag gccggcgccg cgaattccgc ggcgacggcg
     2341 atcagccaga ggatcagccg ggtctgtccc tccaggaagc cgccggcgat ccagagaacg
     2401 gcgcccgcta tggcccagat gcagatgcgc tggaaattgg cgcgcagcac ctcgtgtccc
     2461 cgcaccgccc agagggcgaa aaggcctcgc cccacctgca gcgaggcgaa ggcgcaggcg
     2521 aagatcagac ccttgtcgtc aaacgcctcg ggcagggagg tcgacatcac cagcccggcg
     2581 cccatcagca ggaacagcag ggcccgaacc ggggcgcggt ccgggtccag ccagttggtg
     2641 atccagctgg tgtcgatcca cgcccaccat accgccccca gcagcatgaa accctgcaga
     2701 gccccgatcc agctcaggtg ggccagcaag gcatgcgaaa tctgggtgat ggcgaagacg
     2761 aagatcaggt cgaagaacag ttcgacgaag ccgacgcggg cgtgcccgcc gtcccgggtc
     2821 ctcagcagat cgccc
//
LOCUS       DASVVN010000180         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_453528,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000180 DASVVN010000000
VERSION     DASVVN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453528"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF686_02750"
     CDS             complement(<1..1045)
                     /locus_tag="VF686_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8660760.1"
                     /translation="MVLVQLVQPARPEPAAPSPGELLALARNASPDARQRLLMGVLAL
                     CDASPPSGELSPVLGEIFLTLARQAEREVRKALSEKLAHADWAPSALVNVLALDEIEI
                     ARPILASSPILQDDDLLRVMVEASLEHQIAIARRPQISGRVADAVIDRGEPAVLTALA
                     TNRTADLSVDSVRRLVEHSRRIAALRAPLTRHPRLTEALAEQMYHWVGTALRQSITAR
                     FNVDDKAVGAAVYDAVEGVIRQARADEIPREDPARDEMERRLIDKLQSAGQLRAGFLI
                     RAIREKRLSLFVHGLAALGGFPAEDIREALNASTPEALYYACAAVGIDRAVFPTMLTE
                     LRKLNQSLPGDRGE"
     gene            1180..>1504
                     /locus_tag="VF686_02755"
     CDS             1180..>1504
                     /locus_tag="VF686_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8660761.1"
                     /translation="MADGATTSLKSRVSEAEWNVRVELAALYRLVALHGWDDMIFTHI
                     SARVPGPEHHFLINPYGWYFEEMTASSLVKVDLDGNIVQETTSFINPAGFTIHSAIHA
                     AREDAH"
BASE COUNT          249 a          487 c          536 g          232 t
ORIGIN      
        1 cctcgccccg gtcgccgggc agggactggt tcagcttgcg cagttcggtc agcatggttg
       61 ggaacaccgc gcggtcgatg ccgaccgcgg cgcaggcgta atagagggcc tcgggcgtgg
      121 aggcgttcag ggcctcgcga atgtcttcgg cggggaaacc gcccagcgcg gcgaggccgt
      181 gcacgaacag gctcaggcgt ttctcgcgaa tggcgcggat caggaagccc gcccgcagct
      241 gtcccgcgga ctgcagtttg tcgatcagcc ggcgttccat ctcgtcgcgg gcagggtctt
      301 cccgcggaat ctcatccgca cgggcctgac gaatgacgcc ctcgacggcg tcgtagacgg
      361 ccgcgcccac cgccttgtcg tcgacattga agcgggcggt gatcgactgg cgcaaggccg
      421 ttccgaccca gtggtacatc tgctcggcca gcgcctcggt caggcgcgga tggcgggtca
      481 ggggcgcgcg cagggcggcg atgcggcgcg agtgttcgac cagccggcga acgctgtcga
      541 cgctcaggtc ggcggtccgg ttggtggcca gggcggtcag caccgccggc tcgccgcggt
      601 cgatcaccgc gtcggcgacc cgaccgctga tctggggacg ccgggcgatg gcgatctgat
      661 gctccagcga ggcctcgacc atcacgcgca gcaggtcgtc gtcctgcagg atcgggctgg
      721 aggccagaat cgggcgggcg atctcgatct cgtccagcgc cagcacattg acgagggccg
      781 acggggccca gtctgcgtgg gccagcttct cggacagcgc cttgcgcacc tcgcgctcgg
      841 cctgacgcgc caaggtcagg aagatttcac cgaggaccgg cgagagttcg ccggagggcg
      901 ggctggcgtc gcagagagcc aacacgccca tcaacagccg ctgccgcgcg tccgggcttg
      961 cgttccgggc aagagccaga agctcgccgg ggctgggggc cgcgggttcg gggcgtgctg
     1021 gctggaccag ttgaacgagg accacggacg tctccggcga gccgatagag ttcgacgcag
     1081 ccgagctaac aagcgggacg ctaagacgcg gttaacggaa actgaatctt gacgccgacg
     1141 gggtccggcg cgagcgtgcg cctcaacgag gagacgacca tggccgacgg cgcgacaaca
     1201 tctctcaaat cgagggtgtc agaggcggaa tggaacgtcc gtgtcgaact ggccgccctc
     1261 taccggctgg tggccctgca cggctgggac gacatgatct tcacccatat ctcggcgcgc
     1321 gtgccggggc ccgagcatca tttcctgatc aacccctatg gctggtattt cgaggagatg
     1381 accgcctcgt cgctggtgaa ggtcgatctt gatgggaaca tcgtccagga gacgaccagc
     1441 ttcatcaacc cggcgggctt caccatccat tccgccatcc acgcggcgcg ggaggacgcc
     1501 catt
//
LOCUS       DASVVN010000181         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_348185,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000181 DASVVN010000000
VERSION     DASVVN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348185"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF686_02760"
     CDS             <1..256
                     /locus_tag="VF686_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660762.1"
                     /translation="VVNASWRAPVDGGRRRAQCRVDGDIITWKQVGATNPEQDRWMNQ
                     SGDPVTRFVLDGDAITINTTLPDGSTTTEEYSVAAEQEAR"
     gene            256..2301
                     /gene="nuoG"
                     /locus_tag="VF686_02765"
     CDS             256..2301
                     /gene="nuoG"
                     /locus_tag="VF686_02765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX8660763.1"
                     /translation="MPVAKVNGVETEFQPGMTVLQVAEQAGHEIPRFCYHERLSIAGN
                     CRMCLVEVKPGPPKPQASCALPAAEGQEIFTDTPMVRKAREGVMEFLLINHPLDCPIC
                     DQGGECDLQDQSVGYGRDGSRYAENKRAVEEKNMGPTVKTFMTRCIQCTRCVRFITEV
                     AGVPDIGMISRGEDAEITTYLEKNIDSELSGNVNDLCPVGALTHRPWQYHYRPWELKK
                     TETIDVMDALGSNIRADSKGAEVMRILPRVNEGINEEWLSDVSRYAVDGLQRQRLDQP
                     YVRENGKLRAASWDEALNVVAEKLKAAAADRIGVIAGDLQDAESMKAALDLFRALGSA
                     NTDCRQDGSALGYGPRESWLFNSGLAGVENADAVLIVGANPRTEAPLLNARLRKSWLA
                     GKAEIGLIGEQADLTYEYSYLGAGSKTLGKLPKAAMDALNKAERPAIVVGGSALTGAN
                     GPAVLNALGALAKKIGAVVDGWNGFNVLHSAASRVAGLDMGFVPGEGGKTAGEMLKPG
                     ALDVLFLLGADEVDPTGSDAFRVYLGSHGDRGAHGADVILPGAAYTEKSGLYVNTEGR
                     VQLAERVVFPKGQAKEDWAILRALSARVGQTLPYDTLDALRAKLMADHPTFGRIDYLP
                     APASFDAGSLGRKGDLGDVAFVSSVRDPYLNNPITRASVTMAELSALRIAPVAMAAE"
     gene            2305..3492
                     /gene="nuoH"
                     /locus_tag="VF686_02770"
     CDS             2305..3492
                     /gene="nuoH"
                     /locus_tag="VF686_02770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX8660764.1"
                     /translation="MTDVVPHRSNPLAAVFLTVVGLAVAAGLAVLAVWLWSLIPPSFW
                     TSPVGWLLITAGGILLVTVGILISLAFLLLADRKIWAGVQMRKGPNVVGPFGLLQSFA
                     DFFKFVLKEIIVPAGADKVVFLLAPLLTFVLAFIAWAAIPFAPGWVISDLNVGILYIF
                     AISSLGVYGIIMGGWASNSKYPFLGSLRSAAQMVSYEVSIGLVIINVILLAGSMNLTS
                     IVTQQSGMFWNWFAFGGGADTWPLLVILFPMSIIFFISALAETNRPPFDLPEAESELV
                     AGYQVEYSSTPYLLFMIGEYANIVFMCAMMTLLFFGGWNPGVPQDWLDALPGWLEQGI
                     YFLTFLFKTIFWFTMIAMVKAFVPRYRYDQLMRLGWKIFLPLSLIFVFLVSGWLMIQR
                     VGL"
BASE COUNT          562 a         1148 c         1200 g          610 t
ORIGIN      
        1 ggtcgtcaac gcctcgtggc gcgcgccggt cgacggcggc cgccgccggg cccagtgccg
       61 ggtcgacggc gacatcatca cctggaagca ggtcggcgcc accaaccccg aacaggaccg
      121 ctggatgaac cagtccggcg atcccgtgac gcgcttcgtc cttgacggcg acgccatcac
      181 catcaacacg acgctcccgg atgggagcac gactacggaa gagtattccg tggctgctga
      241 gcaagaggcc cgctgatgcc cgtcgccaag gtcaacggcg tagaaaccga attccagccc
      301 gggatgaccg tgctgcaggt cgccgaacag gccggccacg agattccgcg cttctgctat
      361 catgagcggc tgtcgatcgc gggcaactgc cgcatgtgcc tggtcgaggt gaagcccgga
      421 ccgccgaagc cgcaggcgtc gtgcgccctg ccggccgccg agggccagga gatcttcacc
      481 gacacgccga tggtcaggaa ggcgcgcgaa ggggtgatgg agttcctgct catcaaccac
      541 ccgctggact gcccgatctg cgaccagggc ggcgaatgcg acctgcagga ccagtcggtc
      601 ggctatggcc gcgatggctc gcgctacgcc gagaacaagc gcgcggtcga agaaaagaac
      661 atgggcccga ccgtgaagac gttcatgacg cggtgcatcc aatgcacgcg ttgcgtccgc
      721 ttcatcaccg aggtcgccgg cgtgccggac atcggcatga tctcgcgggg cgaggacgcc
      781 gagatcacca cctatctcga gaagaacatc gactcggagc tgtcgggcaa cgtcaacgac
      841 ctgtgccccg ttggcgccct gacccaccgg ccgtggcagt accactaccg cccgtgggag
      901 ctgaagaaga ccgagaccat cgacgtcatg gacgctctgg gctcgaacat ccgcgccgac
      961 tccaagggcg cggaagtgat gcgcatcctg ccgcgcgtga acgagggcat caacgaggag
     1021 tggctgtccg acgtcagccg ctacgccgtc gacggcctgc aacgccagcg gctggaccag
     1081 ccgtatgtgc gcgagaacgg caagctgcgc gccgcctcgt gggacgaggc gctgaacgtc
     1141 gtggcggaga agctgaaggc ggccgcggcc gaccggatcg gcgtcatcgc cggcgacctg
     1201 caggacgcgg aatcgatgaa ggcggcgctg gacctgttcc gggcgctggg ttccgccaac
     1261 accgactgcc gtcaggacgg atcggcgctg ggctatggcc cgcgcgagag ctggctgttc
     1321 aactcgggtc tggccggggt cgagaacgcc gacgccgtgc tgatcgtggg cgccaatccg
     1381 cgcaccgagg cgccgctgct gaacgcccgt ctgcgcaaga gctggctggc cggcaaggcc
     1441 gagatcggcc tgatcggcga acaggccgat ctgacctatg aatacagcta cctcggcgcg
     1501 ggttcgaaga cgctgggcaa actgcccaag gcggcgatgg acgcgctgaa caaggccgaa
     1561 cggccggcga tcgtcgtcgg cggcagcgcc ctgacgggcg ccaacggccc ggccgtgctg
     1621 aacgccctcg gcgcgctggc gaagaagata ggcgcggtcg tggacggctg gaacggcttc
     1681 aatgtgctgc acagcgccgc ctcgcgcgtg gccggtctgg acatgggctt tgtccccggc
     1741 gagggcggca agaccgcggg tgaaatgctc aagccgggcg cgctggacgt gctgttcctg
     1801 ctgggcgccg acgaggtgga tccgacgggt tcggacgcct tccgcgtcta tctgggcagc
     1861 cacggcgacc gcggcgcgca cggcgccgac gtgatcctgc cgggcgccgc ctataccgag
     1921 aagtcggggc tgtatgtgaa caccgagggc cgagtgcagc tggccgaacg cgtcgtcttc
     1981 cccaaggggc aggcgaaaga ggactgggcc atcctgcggg cgctgtcggc gcgtgtgggc
     2041 cagaccctgc cgtacgacac gctggacgca ctgcgcgcga agctgatggc ggaccatccg
     2101 accttcggcc gcatcgacta tctgccggcg cccgcgtcgt tcgacgccgg ttcgctgggc
     2161 cggaaaggcg atctgggcga cgtcgccttc gtctcgtcgg ttcgcgatcc ctatctgaac
     2221 aacccgatca cgcgcgccag cgtgaccatg gccgagctgt ccgccctgcg gatcgcgccg
     2281 gtcgcgatgg cggcggagta aacgatgacc gacgtcgtac cccatcgttc gaacccgctg
     2341 gccgccgtct tcctgacggt cgtcggcctc gccgtggccg ccggactggc ggttctggcc
     2401 gtctggctgt ggagcctgat cccgccgtcg ttctggacca gcccggtcgg ctggctgctg
     2461 atcacggcgg gcggcatcct gctggtcacc gtcggcatcc tgatctcgct ggccttcctg
     2521 ctgctggccg accggaagat ctgggccggg gtgcagatgc gcaaagggcc caatgtggtc
     2581 gggccgttcg gcctgctgca gtcgttcgcc gacttcttca aattcgtgct caaggaaatc
     2641 atcgtgccgg ccggggccga caaggtggtg ttcctgctgg cgccgctgct gaccttcgtc
     2701 ctggccttca tcgcctgggc ggccattccg ttcgcgccgg gctgggtgat ctcggacctg
     2761 aacgtcggca tcctgtacat cttcgccatc tccagcctcg gcgtgtacgg catcatcatg
     2821 ggcggctggg cttcgaactc gaagtatccg ttcctggggt cgctgcggtc cgcggcgcag
     2881 atggtgtcgt atgaggtctc catcggtctg gtgatcatca acgtcatcct gctggccgga
     2941 tcgatgaacc tgacgtcgat cgtgacccag cagtcgggga tgttctggaa ctggttcgcc
     3001 ttcggcggcg gggcggacac ctggccgctg ctggtcatcc tgttcccgat gtcgatcatc
     3061 ttcttcatct cggccttggc cgagaccaac cggccgccgt tcgacctgcc ggaggcggag
     3121 tccgagctcg tggccggcta tcaggtggaa tacagctcga cgccgtatct gctcttcatg
     3181 atcggcgaat acgccaacat cgtcttcatg tgcgccatga tgaccctgct gttcttcggc
     3241 ggctggaacc ctggcgtgcc gcaggactgg ctggacgccc tgccgggctg gctggagcag
     3301 ggaatctact tcctgacctt cctcttcaag acgatcttct ggttcacgat gatcgccatg
     3361 gtgaaggcct tcgtgccccg ctaccgctat gaccagctga tgcggctggg ctggaaaatc
     3421 ttcctgccgc tgtcgctgat cttcgtgttt ctggtgagtg gctggctgat gatccagcgg
     3481 gtggggctgt gatgctcctc gttgctgcgc tgctgatgca
//
LOCUS       DASVVN010000182         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_506132,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000182 DASVVN010000000
VERSION     DASVVN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506132"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF686_02775"
     CDS             <1..714
                     /locus_tag="VF686_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8660765.1"
                     /translation="SYTSQKAVDYKIGLLMAGGGVLGALLGVELFRYLRLLGQADLAV
                     ALSYLLFLGAIGTLMLYESLGQIMRRVRGEVAPHKERRRPLWLYGLPLKMRFPRSGLY
                     ISAIPPFALGGFVGVLSAIMGVGGGFILVPAMLYVLRMRAGVVVGTSLFQIIIVTAMT
                     TILQAGRNQTVDIVLSTLLLLGGVVGAQYGAKWSGRFRAEELRAVLGLIVLMVGIQMG
                     LELFVRPSDLFAFAPGVAQ"
     gene            711..1520
                     /locus_tag="VF686_02780"
     CDS             711..1520
                     /locus_tag="VF686_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX8660766.1"
                     /translation="MIAALPPPPPAISAPAPERSVGTTDEVRVAAALTDARVRVDSSF
                     RGASIVLYGAVFNPGDQPADVVVVVRGPDGPVRLVKKTRNTGVWLNSRPVLFEGAPGF
                     YMTASTRPLSDIADFGQLRRLGVGVDHLRINAPEESRTVTRYGVRDVVVSRLGEDYLD
                     FRRAVIRLREAAALYNTDPKGVEFVDRGLFRAEVKLPAVAPTGRYFAEVWLFQDGEPV
                     SVSNLTLTVEKVGIERDIFEFAHRRPWTYGLLCVLLAAFTGWAASRLFRRQ"
     gene            1542..1895
                     /locus_tag="VF686_02785"
     CDS             1542..1895
                     /locus_tag="VF686_02785"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8660767.1"
                     /translation="MATPPADIDAYLAGLAGDHATAGQAVRAAILEAAPGASETIRYG
                     MPAFQIGGATIIYFAVWKKHIGLYPIYRGDADYEAALAAYRSKTDTVNLPLAEPLPVE
                     LIGRIVRSQIALARA"
     gene            complement(1892..2995)
                     /gene="hisC"
                     /locus_tag="VF686_02790"
     CDS             complement(1892..2995)
                     /gene="hisC"
                     /locus_tag="VF686_02790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX8660768.1"
                     /translation="MTDASHDAPKPKPGILDIAPYVGGKSRIEGVAEPMKLSSNENAL
                     GAGEKAREAYRSAVDNIHMYPDGRATKLRDAVAEHHGLEPERLIFGNGSDEVFALLNQ
                     TYLTPGDNIVTGQYGFLAYRISALANQAEVKLAPEPGFKAEVDALLAQVDERTKIVYI
                     SSPSNPTGSYNTAEETRRLHAALPPHVLLVIDEAYAEFVSAPDWETSFPLARDSDNIV
                     VTRTFSKIHGLGGLRIGFGYAPLKVAEAVDRIRLPFNVSVPGLEAAVAALGDEAHQKA
                     SRELVEAWRPRLTQAIKGFGFDVFPSAGNFVLVRFPDAARNAAAANDYLNSKGIIVRP
                     VGGYGLPDCLRITVGTEDQNRAVIDALSEFAAS"
BASE COUNT          408 a          974 c         1093 g          522 t
ORIGIN      
        1 agctacacca gccagaaggc ggtcgattac aagatcggcc tgctgatggc cggcggcggc
       61 gttctgggcg cgctgctggg ggtcgaactg ttccgctatc tgcgcctgct gggccaggcc
      121 gatttggcgg tggccctgtc ctatctgctg ttcctcggcg ccatcggcac cctgatgctc
      181 tacgagagtc tgggccagat catgcggcgg gtgcgcggcg aggtggcgcc gcacaaggag
      241 cgccgccggc ccctgtggct gtacggcctg ccgctgaaga tgcgcttccc ccgctcgggc
      301 ctgtacatca gcgccattcc gcctttcgcg ctgggtggct tcgtcggcgt gctgtcggcc
      361 atcatgggtg tgggcggcgg cttcatcctg gtcccggcca tgctgtacgt cctgcgcatg
      421 cgggccgggg tggtggtggg caccagcctg ttccagatca tcatcgtcac cgccatgacc
      481 accatcctgc aggccggacg caaccagacc gtcgacatcg tcctgtcgac cctgctgctg
      541 ctgggcggcg tcgtcggcgc ccagtacggc gccaaatggt cgggccggtt ccgggccgag
      601 gagcttcggg cggtgctggg cctgatcgtg ctgatggtgg gcatccagat ggggctggag
      661 ctgttcgtgc ggccgtcgga tctgttcgcc ttcgcaccgg gggtggccca atgatcgccg
      721 ccctgccgcc gcctccgccc gccatctccg cccccgcgcc ggaacgctcg gtcggcacga
      781 ccgatgaggt gcgcgtcgcc gctgccctga ccgacgcccg ggtcagggtc gattccagct
      841 tccggggtgc gtcgatcgtt ctgtacggcg cggtgttcaa tcccggcgac cagccggcgg
      901 acgtcgtggt ggtggtgcgc gggccggacg ggcccgtgcg tctggtcaag aagacgcgta
      961 acaccggcgt ctggctgaac agccggccgg tgctgttcga gggggccccc ggcttctaca
     1021 tgacggcctc gacgcggccg ctgtcggaca tcgctgattt cggccagctg cgccggctgg
     1081 gcgtcggtgt cgatcacctg cggatcaacg ccccggagga gagccgcacc gtcacccgct
     1141 atggcgtgcg cgacgtggtc gtcagccggc taggcgagga ctatctcgac ttccgccgcg
     1201 cggtgatccg gctgcgcgag gcggcggcgc tgtacaacac ggatcccaag ggggtcgagt
     1261 tcgtcgaccg cggcctgttc cgggccgagg tcaaactgcc ggccgtcgct cccacgggcc
     1321 ggtatttcgc cgaggtctgg ctgttccagg acggcgaacc ggtctcggtc agcaacctga
     1381 ccctgacggt cgaaaaggtc ggcatcgagc gcgacatctt cgagttcgcg catcgccggc
     1441 cgtggacgta cggcctgctt tgcgtcctgc tggcggcctt cacgggctgg gcggcgagcc
     1501 ggctgttccg gcggcagtga cgggcaggga gatcagcagg catggcgact ccgcccgctg
     1561 acatcgacgc ctatctggca gggctggccg gcgaccacgc cacggccggt caggcggttc
     1621 gcgccgcaat tctggaagcg gccccgggtg cgtcagagac gatacgctac ggcatgcccg
     1681 cgtttcagat cggcggcgcc acgatcatct atttcgccgt ctggaagaag catatcggcc
     1741 tctatccgat ctaccggggc gatgccgact atgaggcggc gctggcggcg taccggtcga
     1801 aaaccgacac ggtgaacctg ccgctcgccg agcccctgcc ggtcgagctg atcgggcgga
     1861 tcgtccggtc gcagatcgcc ttggcccgcg cctagctcgc cgcgaactcg ctcagcgcat
     1921 cgatgaccgc ccggttctgg tcctcggtcc ccacggtgat gcgcaggcag tcgggcaggc
     1981 cgtaaccgcc gacggggcgg acgatgatgc ctttcgagtt cagatagtca ttggcggcgg
     2041 cggcgttgcg agccgcgtcc gggaagcgca ccagcacgaa attgccggcg gacgggaaga
     2101 cgtcgaagcc gaagcccttg atggcctggg tcaggcgcgg gcgccacgcc tcgaccagtt
     2161 cgcgcgaagc tttctggtgg gcctcgtcgc cgagagcggc gacggcagcc tcgaggccgg
     2221 ggaccgagac attgaagggc aggcggatgc ggtcgaccgc ctcggccacc ttcagcgggg
     2281 cgtagccgaa gccgatgcgc aggccgccca ggccgtggat cttcgagaag gtgcgggtca
     2341 cgacgatgtt gtcgctgtcg cgggcgaggg ggaagctggt ctcccagtcc ggcgcagaga
     2401 cgaactcggc ataggcctca tcgataacca gcaggacgtg cggcggcagg gccgcgtgca
     2461 ggcggcgggt ttcctcggcg gtgttgtagc tgccggtcgg gttggagggg ctcgagatgt
     2521 agacgatctt cgtccgctca tcgacctgcg ccagcagggc gtcgacctcg gccttgaagc
     2581 cgggctcggg agccagtttg acctcggcct ggttggccag ggcgctgatg cgataggcga
     2641 ggaagccgta ctggccggtg acgatgttgt cgccgggcgt gagataggtc tggttcagca
     2701 gggcgaagac ctcgtccgag ccgttgccga agatcagccg ctcgggttcc agcccgtggt
     2761 gttcggcgac cgcgtcgcgc agcttcgtgg cgcggccatc ggggtacatg tggatgttgt
     2821 cgaccgccga ccggtatgcc tcgcgcgcct tctcgccggc gccgagggcg ttctcgttgg
     2881 acgacagctt catcggctcg gccacgccct cgatgcgcga cttgccgccg acgtaggggg
     2941 cgatgtcgag gatgccgggc ttcggcttcg gggcgtcgtg cgaggcgtcg gtcatgg
//
LOCUS       DASVVN010000183         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_165071,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000183 DASVVN010000000
VERSION     DASVVN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165071"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF686_02795"
     CDS             <1..640
                     /locus_tag="VF686_02795"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HEX8660769.1"
                     /translation="TTADVKAETDICCTSANAVQIVEWAAKEWGVDRVILIPDEFLAR
                     NVAAQTNVGIIAWKGRCIVHERFTASDIAEMREAYPGAEILAHPECPEDVLSAADFAG
                     STAAMTDYITSRKPKQVVLITECSMAANIQGDVPEVSFIGPCNMCPYMKRITLQNIRD
                     CLLHMQFEVTVPEEMAERARLAVQRMIDLPPPAIPARYDLFKARHHVAVELI"
     gene            750..1595
                     /locus_tag="VF686_02800"
     CDS             750..1595
                     /locus_tag="VF686_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8660770.1"
                     /translation="MTDSDQTRRPGPWENLPPVAPPEREIARPQAGEPPLDKGQHPVW
                     AVVSTLLLGGFIWWASGSWVVAVAAIWGLLVHEYGHVLAMNALGMGPARIYIIPFLGG
                     VAKSQRLPASEWHGVLVSLAGPAFGLLAAIPFFGLYIATGQGMWLQGVFVIALINLVN
                     LAPAPPLDGSKALGPVLARIHPMVEKAAMVAVGVIVIVWGFTNGSYIFAAFLALALFG
                     HLKRGIWRPEGRQLNWPEAARSVGLFAIVAAACAGVALAGLMPVTGSFGQAMALAGSY
                     FEFGR"
     gene            1592..3121
                     /locus_tag="VF686_02805"
     CDS             1592..3121
                     /locus_tag="VF686_02805"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX8660771.1"
                     /translation="MSRVRHDGPLIVGGGLAGLSAALEAAPRKVLLVTPAPLLEACSS
                     AWAQGGVAAALSADDAPALHAADTAAAGAGLVETAATQALTGDGRETVEWLAALGAPF
                     DRDADGGFAVSLEAAHSHARVARVGGDGAGRAILSALVTAVRAAPHIEVWEDARLRGL
                     MQDASGRVCGALIERGGGRTVEVTAPAVVLATGGAGGLYARTTTPAALLGEGMALAWA
                     AGADILDPEFVQFHPTAIDVGLDPMPLATEALRGEGARLIDREGRFLLGEEPDADLQP
                     RDVVARAVHAAVADGRGAFLDARAAIGHEFPDAFPAVFAACMRAGLDPRETPIPVAAA
                     AHYHMGGIAAGPDGRTTLPGLFAVGECAATGVHGANRLASNSLLEAAAFGRRTGRAAA
                     AEHGGTGASIAVVAPPGLSDAELQRLRAAMSADVGVVRDADGLTRAVALIDALEQTAG
                     PALPLVAARLIAMAALARRESRGGHFRRDYPAAAAQAQHTRVALAPESGVESGVLAAA
                     G"
     gene            3126..>3973
                     /gene="nadC"
                     /locus_tag="VF686_02810"
     CDS             3126..>3973
                     /gene="nadC"
                     /locus_tag="VF686_02810"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX8660772.1"
                     /translation="MIRQLPDLLIEPVVLMALTEDLGRAGDVTAQACIPDGARMKASF
                     VARKPGVLAGIDCVRLSVLAMDPEASIELKLRDGEAFEAGAVLAEVEADARAFLAAER
                     TALNLLGRLCGVATLTRAYVEAAAGTQARIADTRKTTPGLRALEKHAVQCGGGVNHRF
                     GLDDAILIKDNHVAVCGGVGEAIRRARAAVGHLMKVEVEVDGLDQLDEALAEQPDVIM
                     LDNFTLPMLREAVERAKVSPFGRPVLEASGGVNLETVAGIAATGVDVISVGALTHSAP
                     SLDIGL"
BASE COUNT          519 a         1330 c         1537 g          587 t
ORIGIN      
        1 caccacggcc gacgtgaagg ccgagacgga catctgctgc accagcgcca acgcggtgca
       61 gatcgtcgaa tgggcggcga aggagtgggg tgtcgaccgg gtcatcctga tccccgacga
      121 attcctcgcc cgcaatgtcg cggcccagac caatgtcggc atcatcgcct ggaagggccg
      181 ctgcatcgtg cacgagcggt tcaccgcctc ggacatcgcc gagatgcgcg aggcctatcc
      241 gggcgcggaa atcctggcgc accccgaatg ccccgaagac gtgttgtcgg ccgccgactt
      301 cgccggatcg acggcggcga tgaccgacta catcaccagc cgcaagccga agcaggtggt
      361 gctgatcacc gaatgctcga tggccgccaa catccagggc gacgtgcccg aggtcagctt
      421 catcgggccg tgcaacatgt gcccctatat gaagcggatc acgcttcaga atatacggga
      481 ttgcctgctg cacatgcagt tcgaggtgac ggtgccggaa gagatggccg agcgcgcgcg
      541 gctggcggtg cagcggatga tcgacctgcc gccgccggcg atccccgccc gctacgacct
      601 gttcaaggcc cgacaccatg tggcggtgga gctgatctga tgcactacgt cgcgcccgtc
      661 cgggtcgtcg caaacgtgga tgcgaagagc ctgcggcttc tcgccctcct ggccttgtgg
      721 ctgaggtcgg agaaggccac gtctgcgcga tgacagactc agatcagacg cgccgcccgg
      781 gtccatggga gaacctgccg ccggtcgcgc cgcccgagcg tgaaatcgcg cgtccgcagg
      841 cgggagagcc gccgctggac aagggccagc acccggtctg ggcggtggtc tcgaccctgc
      901 tgctgggtgg tttcatctgg tgggcgtcgg gcagctgggt cgtcgcggtg gcggcgatct
      961 ggggcctgct ggtgcatgag tacggccatg tgctggcgat gaacgccctc ggcatgggcc
     1021 cggcccgcat ctacatcatc cccttccttg gcggcgtggc gaagagccag cgcctgccgg
     1081 ccagcgagtg gcatggcgtg ctggtctcgc tggcggggcc ggcgttcggc ctcctggccg
     1141 ccattccgtt cttcggcctg tacatcgcga cggggcaggg gatgtggctg cagggggtgt
     1201 tcgtcatcgc cctgatcaat ctggtgaatc tggcgcccgc gccgccgctg gacggatcca
     1261 aggcgctggg gccggtgctg gctcgcatcc atccgatggt cgagaaggcc gccatggtcg
     1321 ccgtcggcgt gatcgtgatc gtctggggct tcaccaacgg cagctacatc ttcgccgcct
     1381 tcctggcgct ggccctgttc ggccatctga agcgcgggat ctggcggccg gaggggcgtc
     1441 agctcaactg gcccgaggcg gcgcgcagcg tcggcctgtt cgcgatcgtg gccgcggcct
     1501 gcgcgggggt ggccctggcc gggctgatgc cggtcaccgg gtccttcgga caggcgatgg
     1561 cgctggccgg cagctatttc gagttcgggc gttgagccgc gtccggcatg acggtccgct
     1621 gatcgtcggc ggcggtctgg ccggcctgtc ggccgcgctg gaggccgcgc cgcgcaaggt
     1681 gctgctggtc acgcccgccc ccctgctgga ggcctgctcc tcggcttggg cgcagggtgg
     1741 ggtggccgcg gcgctgtcgg cggacgacgc gccggccctg catgcggccg atacggcggc
     1801 ggcgggcgcc gggctggtcg agaccgcggc gacgcaggcc ctgaccggcg acggccgcga
     1861 aacggtggaa tggctggccg ccctcggcgc gcccttcgac cgcgacgccg acggcggctt
     1921 cgccgtcagt ctggaagccg cccattcgca cgcccgggtc gcccgggtcg gcggcgacgg
     1981 ggcagggcgc gccattctgt ccgcactggt caccgccgta cgcgccgcgc ctcatatcga
     2041 ggtgtgggaa gacgcgcgcc tgcgcggcct gatgcaggac gcctcgggcc gggtgtgcgg
     2101 ggctctgatc gagcgcggcg ggggacggac cgtcgaggtc acggcgcccg ccgtggtgct
     2161 ggccaccggc ggcgccggcg ggctgtacgc ccgcacgacg accccggcgg ccctgctcgg
     2221 cgagggcatg gccctggcct gggcggcggg ggcggacatc ctcgatcccg agttcgtgca
     2281 gtttcatccg acggccatcg acgtggggct ggaccccatg ccgctggcca ccgaggccct
     2341 gcgcggcgag ggcgcgcggc tgatcgaccg cgagggccgt ttcctgctgg gcgaggagcc
     2401 ggacgccgac ctgcagccgc gcgacgtggt cgcccgggcc gttcacgccg ccgtcgccga
     2461 cggacgcggg gccttcctgg acgcccgcgc ggcgatcgga catgagttcc ccgacgcctt
     2521 cccggccgtc ttcgccgcct gcatgcgcgc cggactggac ccgcgcgaga cgccgatccc
     2581 ggtggcggcg gcggcccact atcacatggg ggggatcgcc gccgggccgg acggccgcac
     2641 gaccctgccg ggcctgttcg cggtcggtga atgcgcggcg acgggagtgc acggcgccaa
     2701 ccggctggcg tccaactccc tgctggaggc cgcggcgttt ggacggcgca ccggacgagc
     2761 ggcggcggcg gagcacggcg ggaccggggc gtcgatcgcg gtggtagcgc cgcccggcct
     2821 gagcgacgcg gagctgcagc ggctgcgggc ggccatgagc gccgatgtcg gggttgtgcg
     2881 cgacgccgac ggcctgacgc gcgccgtggc ccttatcgac gcgctggagc aaacggccgg
     2941 accggccctg cctctggtcg cggcgcggtt gatcgccatg gcggcgctgg cgcggcgcga
     3001 aagccggggc gggcatttcc gccgcgacta tccggcggcg gcggcgcagg cgcagcatac
     3061 gcgggtcgct ctggcgcccg agtccggcgt ggagtccggt gttctggccg cggcggggta
     3121 gagacatgat ccggcaactg cccgacctgc tgatcgagcc cgtggtcctg atggccctga
     3181 ccgaagacct cggccgcgcc ggcgatgtga cagcccaggc ctgtatcccc gacggcgcgc
     3241 gcatgaaggc gtcgttcgtg gcgcgaaagc cgggcgtgct ggccgggatc gactgcgtgc
     3301 gcctgagcgt gctggccatg gatcccgagg cgtcgatcga actgaagctg cgcgacggcg
     3361 aagcgttcga ggcgggcgcg gtcctggccg aggtcgaggc ggacgcgcgg gcgtttctgg
     3421 ccgccgaacg gacggcgctg aatctgctgg ggcggctgtg cggggtggcg accctgacgc
     3481 gggcctatgt cgaggcggcg gcgggaacgc aggcgcgcat cgccgacacc cgaaagacga
     3541 cgccggggct cagggcgctg gagaagcatg cggttcagtg cggcggcggg gtcaatcacc
     3601 gcttcggcct ggatgacgcg atcctgatca aggacaacca cgtcgcggtc tgcggcggcg
     3661 tcggcgaggc gatccgccgg gcgcgggcgg cggtcggcca tctgatgaag gtcgaggtcg
     3721 aggtcgacgg gctggatcag ctggacgagg cgctggcgga acagccggac gtcatcatgc
     3781 tggacaattt caccctgccg atgctgcggg aggcggtgga gcgggcgaag gtctcgccct
     3841 tcggccggcc cgtgctggag gcctcgggcg gggtcaatct ggagacggtg gcgggcatcg
     3901 ctgcgaccgg cgtggacgtc atctcggtgg gcgccctgac ccattcggcg ccgtcgctgg
     3961 acatcgggct gga
//
LOCUS       DASVVN010000184         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_6124, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000184 DASVVN010000000
VERSION     DASVVN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6124"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF686_02815"
     CDS             complement(<1..317)
                     /locus_tag="VF686_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660773.1"
                     /translation="MKRIAIAAAVVCWAPAAAAQTAEAPSPVEAPQSAAPGAKTAPLP
                     LDDLDLPPELREMLRGVKVTPELRESLAKVGQAAAEMTPRALTPTGEPAQIASGAVAV
                     QAPV"
     gene            complement(387..1484)
                     /gene="ychF"
                     /locus_tag="VF686_02820"
     CDS             complement(387..1484)
                     /gene="ychF"
                     /locus_tag="VF686_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX8660774.1"
                     /translation="MALKVAIVGLPNVGKSTLFNALTQTAAAQAANYPFCTIEPNVGD
                     VAVPEPRLDKLAAIAKSKEIIPARINFVDVAGLVRGASKGEGLGNQFLANIRDTDAIA
                     FVARCFEGGDVTHVEGRVDPISDLEIIETELMLADLESLEKRVDNLSKRAKTGDKESA
                     ATLKLVNLALEQLNAGRPARAAKVSKEDEKAWRMLQLLTSKPDLYIANVDEESAATGN
                     ALSEKVAERARRDGAKSVVISAKIESEIAMLPQEERADFLEAVGLEEPGLNRLIHEAY
                     ALLGLQTYFTVGPKEARAWTIPVGATAPKAAGEIHTDFEKGFIRAETIAYDDYIRLNG
                     ELGAKEAGKMRLEGKEYVVRDGDVMHFRFNA"
     gene            1621..>2115
                     /locus_tag="VF686_02825"
     CDS             1621..>2115
                     /locus_tag="VF686_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX8660775.1"
                     /translation="MRGLFLGVALLAFGSAAAQEPQADPTGGRIPGQRLAVTIYNNDL
                     ALVEDVRSISPPQGRSRQEFPGVSAQIRPETVSFAADATEVVEQNFDYDLLSPDSLMQ
                     NAVGQEVRIVRTNPGNGQETSERATVLAVNNGVVLRIGDRIEVLRQDSVPTRVIFDRV
                     PPNMR"
BASE COUNT          292 a          730 c          729 g          364 t
ORIGIN      
        1 accggcgcct gcacggccac ggcgccggag gcgatctgcg ccggctcgcc cgtgggcgtg
       61 agcgcgcgcg gcgtcatctc ggcggcggcc tgaccgactt tcgcgaggct ttcgcggagc
      121 tccggcgtca ccttgacccc ccgcagcatc tcgcggagct caggcggcag gtcgaggtca
      181 tccaacggca gcggcgccgt cttggcgccc ggtgctgcgc tctgcggcgc ctcaaccggg
      241 gaaggggcct ccgcggtttg ggcggcggcg gcgggcgccc agcagacaac ggcggcggcg
      301 atcgcgatgc gtttcaaggc tttcccccgg aagatttcgg ggaagggtga accgcgcttc
      361 gccagaggcg gcaacgcggc cgttcgtcac gcattgaagc ggaagtgcat gacgtcgccg
      421 tccctgacga cgtactcctt gccttcgagc cgcatcttgc cggcctcctt ggccccgagc
      481 tcgccgttca gccggatgta gtcgtcgtag gcgatggtct cggcccggat gaagcccttc
      541 tcgaagtcgg tgtggatctc gcccgccgcc ttgggggcgg tggcgcccac ggggatggtc
      601 cacgcccgcg cctccttcgg cccgacggtg aagtaggtct gcagcccgag cagggcgtag
      661 gcctcgtgga tcagccggtt cagccccggc tcctccagcc caacggcctc caggaagtcc
      721 gcccgctcct cttggggcag catggcgatc tcgctctcga tcttggccga gatgaccacg
      781 ctcttggccc cgtcgcgccg ggcgcgctcg gccaccttct cggacagcgc attgccggtc
      841 gccgcgctct cctcgtcgac gttggcgatg tagaggtcgg gcttggacgt cagcagctgc
      901 agcatgcgcc aggccttctc gtcctctttc gaaaccttcg ccgcccgcgc ggggcggccg
      961 gcgttcagct gctccagcgc caggttgacc agcttcaggg tggcggccga ctccttgtcg
     1021 ccggtcttgg cccgcttgga gaggttgtcg acgcgcttct cgaggctctc caggtcggcc
     1081 agcatcagct cggtttcgat gatctcgagg tcgctgatcg ggtcgacgcg gccctccaca
     1141 tgggtgacgt cgccgccctc gaagcagcgg gcgacgaagg cgatcgcgtc ggtgtcgcgg
     1201 atgttggcca ggaactggtt gcccaggccc tcgcccttgg aggcgccgcg caccagtccc
     1261 gccacgtcga cgaagttgat cctcgccgga atgatctcct tcgacttggc gatcgccgcc
     1321 agcttgtcca gccgcggctc ggggacggcc acgtcgccga cgttcggctc gatggtgcag
     1381 aacggatagt tggccgcctg ggccgccgcc gtttgggtca acgcattgaa aagagtggac
     1441 ttgccgacgt tcggcaggcc gacgatcgcg actttcaggg ccatgatgct cgtggagggt
     1501 agggcggcgc ttttatgggg atgtgtcgcc ggaaggacaa cgcctcaccg gtttgtgtca
     1561 tcgacatgcc cgatttgggc tgacagcttg ccggcgggac gatcaggcag gagtgcgttg
     1621 atgcgtgggc tttttctcgg ggtggcgttg ctcgcgttcg gctcggccgc ggcccaggag
     1681 ccgcaggccg atcccaccgg aggccgcatc ccgggccagc ggctggcggt caccatctac
     1741 aacaatgacc tcgccctggt tgaggacgtc cgcagcatca gcccgccgca agggcgctcc
     1801 cgccaggagt tccccggcgt ctcggcccag atccggccgg agacggtctc cttcgcggcc
     1861 gacgccaccg aagtggtcga gcagaacttc gactacgacc tgctctcgcc cgacagcctg
     1921 atgcagaacg ccgtcggcca ggaggtgcgc atcgtgcgca ccaaccccgg caacggccag
     1981 gagaccagcg agcgggccac cgtgctggcg gtcaacaacg gcgtcgtgct gcgcatcggc
     2041 gaccgcatcg aggtgctgcg ccaggacagc gtgccgacgc gggtcatctt cgaccgcgtg
     2101 ccccccaaca tgcgc
//
LOCUS       DASVVN010000185         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_348386,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000185 DASVVN010000000
VERSION     DASVVN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348386"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF686_02830"
     CDS             <1..264
                     /locus_tag="VF686_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660776.1"
                     /translation="NGAADTGFIGDKKETSMTHAPRIPKEQLSFADKGGKSVEDAAKS
                     DRRDLETEIQTGQPGDADVNTKEQGRYGNIKQNTTHQGYQQDR"
     gene            267..494
                     /locus_tag="VF686_02835"
     CDS             267..494
                     /locus_tag="VF686_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660777.1"
                     /translation="MANNQHTPEPVDEKRWGGPGSSHQNADREPPAFEDPREGGPTNP
                     RNSKVSGGGGERDLKHSHDDDHRSSKGHRDA"
     gene            complement(499..1590)
                     /locus_tag="VF686_02840"
     CDS             complement(499..1590)
                     /locus_tag="VF686_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8660778.1"
                     /translation="MPNAPLPCAKTPVDRLARIRPGTAAAAFGVVMGLAVGCAYLGGT
                     AAKATTVRNQAERIEGATAAGFTEEALAAAAGGLDESALAIARRHDPYTVAGGAQRDR
                     QAELMTARLEQLRAQPAHSALRQVSLTGPVAAQPFRLGGALDASRDLECLTQAAYYEA
                     RGEGRDGMRAVTQVVLNRVRHPAFPKSVCSVVFQGAGRRTGCQFSFTCDGSMRGGVNR
                     AAWNRAREVASSALSGSVFAPVGNATFFHTTAVAPGWRNAMVRVGQVGDHLFYRFGGR
                     SGSRGAFTYAARPSGPAEGPRLIQASMDPTATVREAGAVAYNLLVSQETRSAPAETAP
                     APAASAPAPVVVDVPAAAAAPAETTPTAA"
     gene            2054..2239
                     /locus_tag="VF686_02845"
     CDS             2054..2239
                     /locus_tag="VF686_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660779.1"
                     /translation="MKRMAITLVLATVAAAAAGCGHTIEQKAATGAVAGALVAGPVGA
                     AVGGAAGAAVGQAQKPN"
     gene            2399..2761
                     /locus_tag="VF686_02850"
     CDS             2399..2761
                     /locus_tag="VF686_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEX8660780.1"
                     /translation="MRDLKQTGFADRLTTQQEAKKALLARFKAKPAAPDPEFEKLALK
                     RAAEKEALRQQHELAKAEARREKAEKEAARMAADAEAQEAVEAEKRAARKERKALTKE
                     EQKAARDARYAARKARSR"
     gene            complement(2765..>3982)
                     /locus_tag="VF686_02855"
     CDS             complement(2765..>3982)
                     /locus_tag="VF686_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660781.1"
                     /translation="DLKRINELLEERVGEAMAEKAKAEADLMHAQRMEAVGRLTGGVA
                     HDFNNLLTVVIGALDIMLRSPNDAAKRQKLGEAALAAARRGEGLTHQLLAFSRRQALR
                     PEAVDLNAQIRESEPLLRRAVGEAVEFRLKLRRGGARVSVDPSQFEAALLNLVVNARD
                     ALGDKGRITVQTQSCSVEAGEVTDLTAGDYVCVTVTDNGSGMSPEVIARVFEPFFTTK
                     AVGKGTGLGLSQVYGFARQSGGAAHIASTVGRGTTIKLYLPPLAEDGVTAVEAEPGAA
                     QAMKAGRRLLLVEDDASVTVVALELLEGLGLEVRAVETAPQALELLKRERFDIMLSDV
                     VMPGGMTGIELARRCETDHPDMRVVLTSGYAGDDVDAALADAPWPFLRKPYSGEQLAR
                     ILGDLAPEDAAAV"
BASE COUNT          678 a         1349 c         1391 g          564 t
ORIGIN      
        1 aacggggccg ccgacacggg gtttattggg gacaagaagg agacttcgat gacccacgcc
       61 cctcgtattc cgaaagagca gctgagcttc gccgacaagg gcggaaagtc cgtggaggac
      121 gccgcgaaaa gcgaccgtcg cgatctcgag accgagatcc agaccggcca gccgggcgac
      181 gccgacgtga acaccaagga gcagggccgg tacggcaata tcaagcagaa cacgacccac
      241 cagggctatc agcaggatcg gtgatcatgg ccaataacca gcacaccccc gaacccgtcg
      301 acgaaaagcg ctggggcggt cccggctcaa gccatcagaa cgcagaccgc gaaccgccgg
      361 ccttcgagga cccgcgcgaa ggcggaccta cgaacccacg caacagcaag gtctcaggcg
      421 gcggcggcga acgggatctg aagcacagtc acgacgacga ccatcgctcg tcgaagggcc
      481 accgcgacgc ctgacggatc aggcggcggt cggcgtggtc tcggcgggag cggccgcggc
      541 cgcaggaacg tcgacgacga cgggagccgg agcggaagcg gccggggcag gcgcggtttc
      601 agccggggcg ctgcgggtct cctgcgagac cagtaggttg taggcgacgg cgccggcttc
      661 gcggaccgtg gcggtcgggt ccatgctggc ctggatcagg cgggggcctt cggccgggcc
      721 tgacggacgg gcggcgtagg tgaaggcgcc gcgcgagccg gaccgtccgc cgaaacggta
      781 gaacagatgg tcgccgacct ggccgacccg caccatggcg ttgcgccagc ccggggcgac
      841 cgcggtggtg tggaagaagg tggcgttgcc gaccggggcg aagacgctgc cggacagggc
      901 gctggaggcg acttcgcggg cccggttcca ggcggcgcgg ttgacgcctc cgcgcatcga
      961 gccgtcgcag gtgaagctga actggcagcc cgtccggcgg ccggcgccct ggaatacgac
     1021 gctgcagacg ctctttggga aggcggggtg gcggacgcgg ttcaggacaa cctgggtcac
     1081 agcgcgcatg ccgtcgcggc cttcgccacg ggcttcgtaa taggcggcct gggtcaggca
     1141 ctcgagatcg cgcgaggcgt caagggcgcc gccgaggcgg aagggctggg ccgcgaccgg
     1201 gccggtcagg cttacctgac gcagggccga atgggcgggc tgggcgcgca gctgctcgag
     1261 gcgcgcggtc atcagctcgg cctgacggtc gcgctgggcg ccgccggcga cggtgtaggg
     1321 gtcgtggcgg cgggcgatgg ccagcgcgct ttcgtcaagg ccgccggccg cggccgccag
     1381 cgcttcttcg gtgaatccgg ccgccgtggc gccttcgatc cgttctgcct gattgcggac
     1441 ggtggtggcc ttcgccgccg tcccgcccag ataggcgcag cccacggcca gccccatcac
     1501 cacgccgaaa gccgcagccg ccgtgccggg ccggatacgg gccagacggt cgacaggggt
     1561 tttcgcgcac ggaagcgggg cgttcggcaa gggctcagtc ctgttcggca gggcgcagtg
     1621 ccgcccccgg gtagtctcgc agccgggcga cacgctccat tttcgggaac cggtgtcgcg
     1681 atcggcctgc ggtgtatcga cggtcggcga acgggccagc tgtcgaggac gcgccttaag
     1741 ccagccgttt gtggcgccaa tgtggttgat tcgcaagatt cgcgtgcgac gcaacatcgc
     1801 gagtctggcg gcatgtaacg tgaaacggca gcggtcctgt cacatggatt ttgaccataa
     1861 tctatcgttc gtgttctgta tttccaatca caaccacaac acggagccgg gatgattctg
     1921 catagcgatg attaatacgg tcatcatcta ttgtctttgt tatggtttca ttgacgtttt
     1981 taacttggaa agcgaagccg gaaccggcgg tcgggcaggc gcgttccagc tgcgctattg
     2041 ggagaattcc gacatgaaac gcatggcaat cacactcgtc ctggccactg tggcggccgc
     2101 cgccgccggt tgcgggcaca cgatcgagca gaaggccgcc acgggcgccg tggccggagc
     2161 cctggtcgcc ggtccggtcg gcgccgcggt gggcggcgcg gcgggcgcag cggtcggtca
     2221 ggcccagaaa cccaactgac acatttctcc gtcgaatgag cgccgcgcgc ccgatcccgg
     2281 ctcgcaacct tgatttcgcc tcctatatag ggttagccga cgcgcccggg ctgagtgatg
     2341 ctcgcccgag ccgtttcgcg tactccgata accgcacaag gacttcgacg acctccgcat
     2401 gagagatctg aaacaaaccg gcttcgccga ccgcctgacc acccagcagg aggccaagaa
     2461 ggccctgctc gcccgcttca aggccaaacc cgccgcgccc gacccggaat tcgagaagct
     2521 ggcgctgaag cgagccgccg agaaggaagc cctgcgccag cagcacgagc tggccaaggc
     2581 tgaagcgcgc cgtgaaaagg ccgagaagga agccgcgcgc atggcggccg acgccgaggc
     2641 gcaggaagcc gtcgaggccg agaagcgcgc cgcccgcaag gagcgcaagg cgctcaccaa
     2701 ggaagaacag aaggccgccc gcgacgcgcg ttacgccgcc cgcaaggccc gcagccgcta
     2761 acgctcagac cgcagccgca tcctcgggcg cgagatcgcc gaggatgcgc gccagctgtt
     2821 cgcccgaata gggtttgcgc aggaacggcc aaggggcgtc cgcgagggcc gcgtccacgt
     2881 cgtcgcccgc atagccggac gtcaacacca cccgcatgtc cggatggtcg gtctcgcaac
     2941 ggcgggccag ctcgatcccc gtcatgccgc cgggcatgac cacgtcgctc agcatgatgt
     3001 cgaatcgctc gcgcttgagc agctccagcg cctggggcgc ggtttcgacc gcccgcacct
     3061 ccagccccag cccttccagc agttccagcg ccaccacggt gacgctggcg tcgtcctcga
     3121 ccagcagcag acggcgaccc gccttcatgg cctgggccgc accgggctcc gcctcgaccg
     3181 cggtcacgcc atcttcggcc agcgggggca gatagagttt aatggtggtc ccgcgcccga
     3241 ccgtcgaggc gatatgggcg gccccgccgc tctggcgcgc gaaaccatag acctggctca
     3301 gtcccagacc ggtgcccttg ccgaccgcct tggttgtgaa gaagggctcg aacacccggg
     3361 cgatcacctc gggggacatg cccgagccgt tgtccgtgac ggtcacgcag acatagtcgc
     3421 cggccgtcag atccgtcacc tctccggcct cgaccgagca cgactgggtc tggaccgtga
     3481 tccgcccctt gtcgccaagg gcgtcgcggg cgttgacgac cagattcagc agggcggcct
     3541 cgaactgcga cgggtcgacg ctgacccggg cgccgccgcg gcggagcttc agcctgaact
     3601 cgacggcttc gccgacggcg cggcgcagca ggggctcgct ctcgcggatc tgggcgttca
     3661 gatcgaccgc ctcggggcgc agggcctgcc ggcgcgagaa ggccagcaac tggtgcgtca
     3721 ggccctcgcc gcgccgcgcg gcggccaggg cggcctcgcc cagcttctgg cgtttggccg
     3781 cgtcgttcgg cgagcgcagc atgatgtcga gggcgccgat caccacggtg agcagattgt
     3841 tgaagtcgtg cgccaccccg ccggtcagcc ggccgaccgc ctccatccgc tgggcgtgca
     3901 tcaggtccgc ctccgccttg gccttctcgg ccatggcctc gccgacccgc tcctccagca
     3961 gttcgttgat ccgcttcagg tc
//
LOCUS       DASVVN010000186         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_375928,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000186 DASVVN010000000
VERSION     DASVVN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375928"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF686_02860"
     CDS             complement(<1..312)
                     /locus_tag="VF686_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8660782.1"
                     /translation="MPKLTSAIDPHSPAFKALDAHNRALRDELHARAAKAARGGSDAS
                     RERHVSRGKLLPRDRVDRLLDPGSPFLEVGQLAANGMYGDAKDPDGAPGAGMICGVGR
                     VS"
     gene            421..1770
                     /locus_tag="VF686_02865"
     CDS             421..1770
                     /locus_tag="VF686_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8660783.1"
                     /translation="MIGFSTQTRKTLTELLHLAWPVVLARIGIMTMGLTDVIVVGNYS
                     GIELAYSSLALAPTAIIVTTAVGLMMGVQVMTARLIGEGRRGEVGGVLRRGVVYSMQI
                     GFASMIGLILIGPWALQHMGLAPGLGEGASPVLMVFALSMPGYLVSVAAQFFLEALHR
                     PRAGMWAMWIANGLNLALNLLLVPDILGIGLDGAVASAWATFVARTSLAVFLLVYIVR
                     LPEARALGVFRRPDRNRVDEREQRKVGYGAGSSYFIEVGAFAAMTFIAGQLGAIETAA
                     WAIVLNVSAIVFMVPMGLSAATAVLVGRAYGARDGPGVLRAGLVGIGVVVGLTLVVAL
                     FVWPGAPLIASAYNRDPELLAVVIPALVLTTLFFVADGIQVVSASANRAAGDVWWPTI
                     MHFAAYSLIMMPLGWWWAHEIGVDGLVWAVIVASLVSSVLLTGRFIRVARRLPDRAL"
     gene            1816..2685
                     /locus_tag="VF686_02870"
     CDS             1816..2685
                     /locus_tag="VF686_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660784.1"
                     /translation="MRKSVSGLAVFAAAGVLLCGCDAAAQDQSLSGAARDARIAELEL
                     QATRGQAVPLPANAAALDDALARRDYTALRSQMGASSSAEAELMLNWAKIAIFKGGSF
                     SVSLIYTGDLWRMAEAMERAAGRDPTNAAALNQQARSLKKLSVMMGLHAEALLQVDAV
                     RCVDSDAAMGMMAETHGALAPQWTFARSLPVEERAVLIRTASILESTIAPVREPDYRL
                     CASGTGMSAMAAGTPRAIEDPATPGADVAVEIDPEDLVIDNGQWWAISPMARTLALTT
                     AARNLEVPFEQVL"
     gene            2682..3599
                     /locus_tag="VF686_02875"
     CDS             2682..3599
                     /locus_tag="VF686_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660785.1"
                     /translation="MTYLNQPPRIDMRGSAPTLAALAAAACLFAGAASAQETESFAGE
                     AREARIAELRQLGARGGEGPMPANEAALDEALARGDYAGLRALMAFETQDEAARILAW
                     NRKVLFEGAPFALAYFYAIDLWSLATSYEAHAAAGGEATPADLEMARSAKLLSVVVAI
                     YARTVVYVDSRRCEDRSVRYQVLADLDAHLAPQWAFVDTLPGELADGMGEAGRGLEER
                     TAPIRQPDLWLCASGYGFSNAQIRAGQDRTGSETARRPLEDLTTPGGDIAREMGRTDL
                     VVNNGLWWAHVPLARIAAMRHAAVDVSGR"
BASE COUNT          505 a         1170 c         1318 g          624 t
ORIGIN      
        1 tgagactcgg ccgacgccgc agatcatgcc ggcgccgggc gcgccgtcgg gatcctttgc
       61 atccccgtac atgccgttgg cggccagctg gccgacctcg aggaagggcg agccgggatc
      121 gagcaggcgg tcgacgcggt cgcgcggcag cagcttgccg cgcgagacgt ggcgttcacg
      181 cgacgcatcg gacccgccgc gcgccgcctt ggccgcccgg gcgtggagtt cgtcccggag
      241 cgcccggttg tgggcgtcca gagccttgaa ggcggggctg tgcggatcga tcgcggaggt
      301 cagcttgggc atggggtcgg tttaagcggc ttcgagggca gggggaagcg actctcctta
      361 tgggcgccca aggaatgccg ggttgacctt gaaccgccgc cgtggcctgt cgcccgtccc
      421 atgatcggct tcagcaccca gacccgtaag accttgacgg aactgctcca cctcgcgtgg
      481 ccggtggtgt tggcgcgcat cggcatcatg accatgggcc tgaccgacgt gatcgtggtc
      541 ggcaactatt cggggatcga gctggcgtac agctcgctgg ccctggcccc cacggccatc
      601 atcgtcacca ccgccgtggg cctgatgatg ggggtgcagg tcatgaccgc ccgcctgatc
      661 ggcgagggcc ggcgcggcga ggtcggcggc gtgctgcgcc gcggcgtggt ctattcgatg
      721 cagatcggct tcgcgtcgat gatcggcctg atcctgatcg ggccgtgggc cttgcagcac
      781 atgggtcttg cgcccgggct gggcgaggga gcctcgcccg tgctgatggt cttcgccctg
      841 tccatgcccg gctatctggt cagcgtggcg gcgcaattct tccttgaggc cctgcaccgg
      901 ccgcgcgccg gcatgtgggc catgtggatc gccaacggcc tgaacctggc gctgaacctg
      961 ctgctggtgc ccgacatcct tggtatcggg ctcgacggcg ccgtcgcctc ggcctgggcc
     1021 accttcgtcg cccggacgtc actggccgtc ttcctgctgg tctatatcgt ccggctgccc
     1081 gaggcgcggg cgctgggcgt gttccggcgg cccgaccgaa accgtgtcga cgagcgcgaa
     1141 cagcgcaagg tcggctacgg ggccggcagt tcgtatttca tcgaggtggg ggccttcgcc
     1201 gccatgacct tcatcgccgg ccagctgggc gcgatcgaga ccgccgcctg ggccatcgtg
     1261 ctgaacgtct cggccatcgt cttcatggtc ccgatggggt tgtcggcggc cacggccgtg
     1321 ctggtcggcc gcgcctatgg cgcgcgggac gggccgggcg tgctgcgggc cggtctggtc
     1381 gggatcgggg tggtggtcgg cctgacgctt gtcgtggcgc tgttcgtctg gccgggcgcg
     1441 ccgctgatcg ccagcgccta caaccgcgat ccggaactgc tggcggtggt cataccggcg
     1501 ctggtgctga cgaccctgtt cttcgtggcg gacggcattc aggtggtctc ggcctcggcc
     1561 aatcgcgcgg cgggtgacgt ctggtggccg acgatcatgc atttcgcggc ctacagcctg
     1621 atcatgatgc cgctgggctg gtggtgggcg cacgagatcg gcgtcgacgg cctggtctgg
     1681 gccgtgatcg tcgccagtct ggtgtcgtcg gtgttgttga cgggacgctt catccgcgtg
     1741 gcgcgcagat tgcccgaccg ggcgttgtaa atcgcgccgc gccccggcaa ggtcacgact
     1801 ggtcgggggt ggtgcatgcg caaatcagta tcgggtctcg ccgtgttcgc ggcggcaggc
     1861 gtccttcttt gcggatgcga cgcggcggcg caggaccagt cgctgtccgg agctgcccgc
     1921 gacgcccgga ttgccgaatt ggagcttcag gccacacgcg gacaggctgt gccccttccc
     1981 gccaacgccg ccgctctgga cgacgcattg gcacgacgcg actacacggc cctgcgcagc
     2041 cagatgggcg ccagcagttc ggccgaggcg gaactgatgc tcaactgggc gaagatcgcc
     2101 attttcaagg gcggttcgtt ctcggtcagc ctcatctaca ccggggacct gtggcgaatg
     2161 gccgaggcga tggagcgggc ggccggacgc gatccgacga atgcggctgc cctgaaccag
     2221 caggcccggt cgttgaagaa gctgtcggtg atgatggggc tgcacgccga agccctcctt
     2281 caggtggatg cggtgcgatg cgtggactcc gacgccgcga tggggatgat ggcggaaacc
     2341 cacggcgcgc tggctcccca gtggaccttc gcgcgatcgc tgccagtcga ggagcgcgcc
     2401 gtcctgatcc gcacggcgtc gattctggaa tcgacaatcg ctccggtgcg ggagccggat
     2461 taccggctct gtgcgagcgg gaccgggatg agcgccatgg cggctggaac gccgcgcgct
     2521 atcgaggacc ccgccacgcc cggcgccgac gtggcggtgg aaatcgatcc ggaggatctg
     2581 gtgatcgata acggccagtg gtgggccatc agtcccatgg cccgtacgct ggccctcacg
     2641 acggcggccc gaaatctcga agtcccgttc gaacaggtcc tgtgacctac ctcaaccagc
     2701 cgccgcgaat tgatatgcgt gggtccgcac cgactctcgc cgcccttgcc gcggcggcat
     2761 gcctgttcgc cggagccgct tcagcgcagg agaccgagtc cttcgccggt gaggcgcggg
     2821 aggcgcggat tgccgaactg cggcagttgg gagcacgggg cggggagggg cccatgccgg
     2881 ccaacgaagc cgccctggac gaggcgctgg cgcgtggcga ctacgcgggg ctccgcgcgc
     2941 tgatggcctt cgagacccag gatgaagccg cgcgaatatt ggcctggaac cgcaaggtcc
     3001 tgttcgaggg cgcgcctttc gctctcgcct atttctacgc gatcgacctg tggagccttg
     3061 cgacgtcgta cgaggcgcat gcggcggctg gaggcgaggc gacccccgcc gatctggaga
     3121 tggcgcggtc cgccaaactc ttgtcagtcg tggtggcgat atacgccagg acggtggtct
     3181 atgtggacag ccgccggtgt gaggaccggt cggtcaggta ccaggtgctc gcagaccttg
     3241 atgcccatct agccccgcag tgggccttcg tcgacacatt gcccggtgaa ctcgcggacg
     3301 gcatgggcga ggcgggtcgc gggcttgagg aaaggacggc gcccatccgc cagcctgatc
     3361 tgtggctttg cgccagtggg tacgggttct cgaacgccca gatcagggcc ggccaggatc
     3421 gcaccgggtc cgaaacagcc aggcggccgc tcgaggatct gacgacgccc ggcggagaca
     3481 tcgcgcgtga aatggggcgg accgatcttg tcgtcaacaa cggtctgtgg tgggcccacg
     3541 tgcccctcgc ccggatcgcc gccatgcggc acgcggccgt cgacgtctcc ggacggtaaa
     3601 cctcgatcgg gccccgg
//
LOCUS       DASVVN010000187         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_427476,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000187 DASVVN010000000
VERSION     DASVVN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427476"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VF686_02880"
     CDS             <1..877
                     /locus_tag="VF686_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660786.1"
                     /translation="RLSADREGRVAFEAFDVRSEIVRVTGDARTPPTASRGESAYPHL
                     SPDGQSLLFSSDRSGAPAVWVMGVGAGSSPANRLISGDYDYVAWPRWSPSGRQIAFVA
                     TTGSRSQLNVAAADGGRIRTAFETANTLWSPQWISEDTVVLAINRSGIWRLWKIRVTD
                     GQATPVSPPGWDSVRVGPDGRLYGTQFAADGVWRVPANEAETPTLIAPLAPIEWRNWE
                     IDGNEVITVRRSETGVDLVATPLSGGGARVVGKPAGVAGLSGVTGDAVHGFYVTRGEM
                     NESDIVVLDTVRRRD"
     gene            996..>1713
                     /locus_tag="VF686_02885"
     CDS             996..>1713
                     /locus_tag="VF686_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8660787.1"
                     /translation="MRARLVSLAALLAVVAPPLTIAGSAAAQTAAVAGSAQAPELRAA
                     PVAELVSEVDIPWKRFTLDNGLTVIVHEDRKAPVVAVSVWYNVGSKDEPAGSTGFAHL
                     FEHLMFGGSENAPGSYLGRMRNLGPSNLNGTTWFDRTNYFQTVPTPALEQALFLESDR
                     MGYLLGAVGQEVLDLQRGVVQNEKRQGDNQPYGLVYYKLLEELLAGSPYGHNVIGSMA
                     DLDAASLDDVKNWFRSHYGPN"
BASE COUNT          278 a          557 c          566 g          312 t
ORIGIN      
        1 acggctgtca gccgatcgcg aaggacgggt tgctttcgag gcgttcgacg ttcgttcgga
       61 gatcgtccgc gtcacgggcg acgcccggac gccgccaacc gcttccaggg gtgaatccgc
      121 ctatccccat ctgtcgccag atggacagtc ccttctgttc tcgtcggatc gcagcggtgc
      181 gccggccgtc tgggtgatgg gtgtcggggc cggatcctcg ccggccaatc gcctgatctc
      241 gggcgattac gactatgtcg cctggccccg ctggtcccca tccggcaggc agatcgcctt
      301 cgtcgcaacc acaggaagcc ggtcgcagct caatgtcgcc gccgcggacg gcgggcgaat
      361 ccggacggcg ttcgaaacgg cgaacacgct gtggagtccc caatggattt ccgaagacac
      421 agtggttctg gcgatcaatc gttcgggaat ctggcggctc tggaaaatcc gggtgaccga
      481 cggccaggcg accccggtgt ctccgccagg ctgggactcg gtccgggtgg ggccggacgg
      541 acggttgtac ggcacgcagt tcgccgctga cggcgtgtgg cgggtccccg ccaacgaagc
      601 cgagacgccg acgctgatcg cgccgttggc ccctatcgag tggcggaact gggaaatcga
      661 cggcaacgag gtcattacgg tccggcgcag cgaaacgggc gtcgatctgg tcgcaacccc
      721 gctttccggc ggcggcgcca gagtggtcgg aaaaccggcc ggcgttgcag gcctttccgg
      781 ggttaccggc gatgcggtgc atggcttcta tgtgacccgc ggcgagatga atgaatcgga
      841 catcgtcgtg ctcgacaccg ttcggcggcg cgactgagtc atcacaggct tgcgtcggcc
      901 gatttgagcg acgctacagt tacgtttgac gaaggtcgcg gcggcatgcc ctatgccgct
      961 acccgtcttc ggcctgaatc attcgaaagt cgtccatgcg cgctcgtctc gtctctctcg
     1021 cggcccttct tgcggttgtc gcgccgcccc tgaccatcgc cggctccgcc gccgcccaga
     1081 ctgcggctgt cgccggttcg gcgcaggccc cggaactgcg cgccgccccg gtcgccgaac
     1141 tggtgtcgga ggtcgacatt ccgtggaagc gcttcaccct cgacaacggc ctgacggtca
     1201 ttgtgcatga ggaccgcaag gcgccggtcg tggccgtgtc ggtctggtac aacgtcggct
     1261 ccaaggacga gccggccggc tcgaccggct tcgcccatct gttcgaacat ctgatgttcg
     1321 gcgggtccga gaatgcgccg ggcagctatc tgggccgcat gaggaatctc gggccaagca
     1381 acctgaacgg caccacctgg ttcgaccgca ccaactattt ccagaccgtg ccgacgccgg
     1441 cgctggagca agccctgttc ctggaaagtg accgcatggg ctatctgctc ggcgccgttg
     1501 gtcaggaggt tctcgatctg cagcgcggcg tggtccagaa cgagaagcgc cagggcgaca
     1561 accagcctta tggcctggtc tattacaagc tgctcgagga gctgctcgcg ggaagcccct
     1621 atgggcacaa tgtgatcggt tcgatggccg atctcgatgc ggccagcctc gacgacgtca
     1681 agaactggtt ccgctcgcat tacggcccca aca
//
LOCUS       DASVVN010000188         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_348452,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000188 DASVVN010000000
VERSION     DASVVN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348452"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF686_02890"
     CDS             complement(<1..208)
                     /locus_tag="VF686_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660788.1"
                     /translation="MRSDASGAKPKYGYIAITFSATARCGVCSQSSGEKVGTKANPAV
                     AGFRKFGAPTVPNPAKLQSSSRAGS"
     gene            250..1293
                     /locus_tag="VF686_02895"
     CDS             250..1293
                     /locus_tag="VF686_02895"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8660789.1"
                     /translation="MNAHVSITPSGPGLLTPSAAYKPFRYPWAFDMWKKQQQVHWMPE
                     EVPLGEDMKDWAVNLTDSERNLLTQIFRFFTQADIEVQDNYMEKYGRVFKPTEVKMML
                     ASFANMETIHIAAYALLLETIGMPESEFAAFLEYEAMRDKHDFMGRFGVETNGDIART
                     LAMFGGFSEGLQLFASFAMLMNFPRQNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFN
                     KETGAVTPQVAADIVDCCKTVVEMEDRFIDLAFEMGPVNGMTPDDIKQYIRFIADWRL
                     RQLQLPEVYGIKENPLPWLQSLLSGVEHANFFEARATEYSKAATTGAWHGAEGVWGSF
                     DAMMKKRAEGAEA"
     gene            1298..2341
                     /locus_tag="VF686_02900"
     CDS             1298..2341
                     /locus_tag="VF686_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8660790.1"
                     /translation="MNRPANVAVVGFGNAGRTFHAPLVAAEPGLTLHTIVSSRPAEVA
                     AVWPATGVAPSLEAVLDDPAIDLVVLATPDALHAAQAEAALNAGKAVVVDKPFALTCA
                     DAARVVELARSKGLLLSVFHNRRWDADFLALKAEIASGRLGRIVTVETRYDRHRPVVR
                     DRWREGPGGGVWLDLGPHLIDQMLVLFGRPLGVTCDLSIQRERGLSPDRARATLRYAD
                     GLRVELNAAMLIAAPDVRFAVHGTRGSWLKSGLDVQEDQLKAGMPVGAPGWGVDPAPA
                     TFVDGETGARAAAPGPAGDYPAFYAGIAVALRGEGPNPVPPEQALAVMEIIDAGLESD
                     RRRAEVQIPANHS"
     gene            complement(2317..3075)
                     /locus_tag="VF686_02905"
     CDS             complement(2317..3075)
                     /locus_tag="VF686_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX8660791.1"
                     /translation="MNRRRWLTGAVATAATALLSACGQAGTSLAEQARRMGEGLTRRA
                     QRLLIPREALAPTYSRAEISPDFRANGSIDPAAADYVALKANGFADYRLVIDGLVERP
                     LSLGLAELRARPAQSQITKHDCVEGWTSIAEWTGVRLETLLDEAGLKPEAKYIVFHCF
                     DALDQREDGERYYESIDLIDARHPQTILAWAMNGETLPVPHGAPLRLRVERQIGYKHA
                     KYIKRIEAVASFADIGGGKGGYWEDRGYEWFAGI"
     gene            complement(3072..>3357)
                     /locus_tag="VF686_02910"
     CDS             complement(3072..>3357)
                     /locus_tag="VF686_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX8660792.1"
                     /translation="LPMMLITGLSMSPGFNAVGGVLLEIMGGRQSARTLHFISAGLIV
                     GFIVVHVGLVVWTGPVNNMRSMITGWFVIGPPGSTAGPKRRRRADDGDAS"
BASE COUNT          549 a         1200 c         1084 g          524 t
ORIGIN      
        1 cggatccggc gcgggacgag gactgaagct tggcagggtt tggcacggtt ggcgccccga
       61 atttccgaaa ccctgccacg gccgggttcg ctttcgtccc caccttctcc ccagaagact
      121 gcgagcagac accacatcta gcggtcgcgg aaaaagtgat cgcaatatag ccgtattttg
      181 gcttggcgcc ggacgcgtcc gaacgcatgg tgctttcacc ctcggattcg ccgacagagc
      241 ccagtaccga tgaacgcgca cgtctcgatc accccgtccg gccccggcct gctgaccccc
      301 tcggcggcct acaagccctt ccgctacccg tgggcgttcg acatgtggaa gaagcagcaa
      361 caggtccact ggatgcccga agaggtgccg ctgggcgagg acatgaagga ctgggcggtc
      421 aatctgaccg acagcgagcg caacctgctg acccagatct tccgcttctt cacccaggcg
      481 gacatcgagg tccaggacaa ctacatggaa aaatacggtc gggtgttcaa accgaccgag
      541 gtcaagatga tgctggcgtc cttcgccaac atggagacca tccacatcgc cgcctacgcc
      601 ctgctgctcg aaaccatcgg catgcccgag agcgagttcg cggccttcct cgaatacgag
      661 gccatgcgcg acaagcatga tttcatgggt cggttcgggg tcgagaccaa cggcgacatc
      721 gcccggaccc tggccatgtt cggcggcttc tcggaggggc tgcagctgtt cgccagcttc
      781 gcgatgctga tgaacttccc gcgccagaac aagatgaagg ggatgggtca gatcgtcagc
      841 tggtcggtcc gcgacgagag cctgcactgc gaaggcatca tcaaactgta ccacgccttc
      901 aacaaggaga ccggcgcggt cacgccccag gtcgccgccg atatcgtcga ctgctgcaag
      961 acggtggtcg agatggagga ccgcttcatc gacctggcct tcgaaatggg cccggtgaac
     1021 ggcatgacgc cggacgacat caaacagtac atccgcttca tcgccgactg gcgcctgcgc
     1081 caactacagc tgcccgaggt ctacgggatc aaggaaaacc cgctgccctg gctgcagtcc
     1141 ctgctgtcgg gcgtcgagca cgccaacttc ttcgaggccc gcgccaccga atactccaag
     1201 gccgccacca ccggcgcctg gcacggcgcc gagggcgtct ggggcagttt cgacgccatg
     1261 atgaagaagc gcgccgaggg cgcggaggcc tgaccccttg aaccggcccg cgaacgtcgc
     1321 cgtcgtcggc ttcggaaacg cgggccggac cttccacgcc ccccttgtcg cggccgagcc
     1381 cggcctgacg ctgcacacga tcgtcagcag ccggcccgcc gaggtggcgg ccgtctggcc
     1441 cgcgaccggg gtcgccccgt cgcttgaggc ggtgctcgac gatcccgcga ttgatctggt
     1501 cgtcctcgcc acgcccgacg ccctgcacgc ggcccaggcc gaggcggcgc tgaacgccgg
     1561 caaggcggtg gtcgtcgaca agcccttcgc cctgacctgt gccgacgccg cccgggtcgt
     1621 ggaactggcc cggtcgaagg gtctgttgct gtcggtcttc cacaaccgcc gctgggacgc
     1681 cgatttcctc gccctcaagg ccgagatcgc cagcggccgg ctgggccgca tcgtcacggt
     1741 cgagacccgc tacgaccgcc atcgccccgt cgtccgggac cgctggcgcg aggggccggg
     1801 cggcggcgtc tggctggatc tgggcccgca tctgatcgac cagatgctgg tcctgttcgg
     1861 ccgtccgctg ggcgtcacct gcgacctgtc gatccagcgc gaacgcggtc tcagcccgga
     1921 ccgggctcgc gccaccctgc gctacgcgga cggcctgcgg gtcgagctga acgccgccat
     1981 gctcatcgcc gcccccgacg tccgcttcgc cgtccacgga acgcggggca gctggctcaa
     2041 gtccggcctc gacgtgcagg aagaccaact gaaggccggg atgcccgtgg gcgcgcccgg
     2101 ctggggcgtc gacccggccc cggcgacctt cgtcgacggc gagaccggcg cccgcgccgc
     2161 cgcgcccggc ccggctggcg actatccggc cttctatgcc gggatcgccg tcgccctgcg
     2221 cggcgagggc cccaaccccg ttccgccgga gcaggcgctg gcggtgatgg agatcatcga
     2281 cgccggactg gaaagcgacc gccgccgggc cgaggttcag atcccggcaa accactcata
     2341 gccgcggtct tcccaatagc cgcccttgcc gccgccgatg tcggcgaagc tggcgaccgc
     2401 ctcgatccgc ttgatgtatt tggcgtgttt gtagccgatc tgccgctcga ccctgagccg
     2461 caagggcgcg ccatgcggaa caggcagggt ctcgccgttc atggcccagg ccagaatggt
     2521 ctgtggatgg cgggcgtcga tcaggtcgat gctctcgtag taccgctccc cgtcctcgcg
     2581 ctgatccagg gcgtcgaaac agtgaaagac gatgtacttc gcctccggtt tcagtcctgc
     2641 ctcgtccagc agggtctcca gccgcacccc ggtccattcg gcgatcgagg tccagccctc
     2701 gacgcagtcg tgtttggtga tctgcgactg cgccgggcgc gcccgaagtt ccgccagccc
     2761 cagcgacagc ggccgttcca ccagtccgtc gatgaccagc cgatagtccg cgaagccatt
     2821 ggccttcagc gccacatagt ccgccgccgc cggatcgatc gagccattgg cccggaagtc
     2881 gggcgatatc tccgcccggc tgtaggtcgg ggcgagggcc tcgcgcggga tcagcaggcg
     2941 ttgcgcccgc cgcgtcagcc cctcgcccat gcgccgcgcc tgttcggcca gactggtccc
     3001 cgcctgaccg caggcggaca gcagggcggt tgccgccgtc gcgacggcgc cggtcagcca
     3061 gcggcggcgg ttcatgaggc gtctccgtca tcggcccggc ggcggcgctt cgggcccgcc
     3121 gtcgagcccg gcggcccgat cacgaaccag ccggtgatca tcgaccgcat gttgttgacc
     3181 ggccccgtcc agaccaccag cccgacgtgg acgacgatga agcccacgat gaggcccgct
     3241 gagatgaaat gcagcgttcg cgccgattgc cgcccgccca tgatctccag caggactccg
     3301 ccgacggcgt tgaacccggg cgacatcgac agacccgtga tcagcatcat cggcagg
//
LOCUS       DASVVN010000189         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_217413,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000189 DASVVN010000000
VERSION     DASVVN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217413"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1236)
                     /gene="secF"
                     /locus_tag="VF686_02915"
     CDS             complement(226..1236)
                     /gene="secF"
                     /locus_tag="VF686_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8660793.1"
                     /translation="MRWPLIKYLPEKTNLRFVAFARLAAALSVLAVIASLFLTIYPAK
                     PPCGGLNCGVDFKGGTVLEMSTPGRAVDLGLVRSTLNGMNLGDVQVQAFGSTSDAIVR
                     FETPSGQNPAAVVTRVKEQLARRVGQVTFSRTEVVGPKVSGELFQGGVMALFIAILLM
                     MFYIWFRFGITFGIGAVAALFHDVILTFGLFAVTRMEFTLVAVAAILTIIGYSMNDTV
                     VVLDRVRENLRKFKKTPLRELIDLSINETLSRTLVTGVTGLMALAGLAVFGGEALFAF
                     SVAMIFGIIIGTYSSIFVAAPVLLLLGVRGRDEQVEPIKPQPSTPGPADRERTRRVVP
                     AP"
     gene            complement(1244..>1884)
                     /locus_tag="VF686_02920"
     CDS             complement(1244..>1884)
                     /locus_tag="VF686_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HEX8660794.1"
                     /translation="SAQISGNFTVETARDLAILLQAGALPAPINVEQQSTVGAELGQD
                     AIEAGKLSTVLAFVAVLGFMVLAYGLLFGGISIVALLVNGLLIIAALSLTGAALSLPG
                     IAGLILTLAVAVDANVLIYERMREEVRAGRPPIVAADAGFARAMVTILDANITTLVAA
                     LIMFQFGAGPVRGFAWTLTIGVFTTLFSAVLISQVLIAWWFKAVRPKKLPIQ"
BASE COUNT          344 a          637 c          652 g          251 t
ORIGIN      
        1 atgccgaaga agacgtgcat ccagcgcagg acgccctgcc cccactggtc gccgaacccg
       61 tccgggttgg tcgccccgaa ggcgtagatc agcacgatcg cgagcagcac gctcacgacc
      121 atggtgttgc ggaagttctg aagcagcccg gccatacgag tccctcccaa ggcgttggcg
      181 cgactctagg agcttggccg cgccggggaa agccgtcgca cacgcctagg gggccggaac
      241 cacccggcgg gtgcgctcgc gatcggccgg gccgggggtg gagggctgcg gcttgatcgg
      301 ctccacctgc tcgtcgcgcc ccctgacgcc cagcagcagc agcaccgggg cggcgacgaa
      361 gatcgacgag taggtgccga tgatgatgcc gaagatcatg gccacggaga aggcgaacag
      421 cgcctcgccg ccgaacaccg ccaggccggc cagcgccatc aggccggtga cgccggtgac
      481 cagggtgcgc gacagcgtct cgttgatcga caggtcgatc agctcgcgga gcggggtctt
      541 cttgaacttg cgcaggttct cgcgcacgcg gtcgagcacg accacggtgt cgttcatcga
      601 gtagccgatg atggtcagga tcgcggcgac ggccaccagc gtgaactcca tccgcgtcac
      661 cgcgaacagg ccgaaggtca ggatgacgtc gtggaacagg gcggccacgg cgccgatgcc
      721 gaaggtgatg ccgaagcgga accagatgta gaacatcatc agcaggatgg cgatgaacag
      781 ggccatcacg ccgccctgga acaactcgcc ggaaaccttc ggaccgacga cttccgtgcg
      841 cgagaacgtg acctgtccga cgcggcgcgc gagctgctcc ttcacgcgcg tgacgaccgc
      901 agccgggttc tggccgctcg gggtctcgaa gcgcacgatg gcgtcgctgg tggagccgaa
      961 cgcctgcacc tgcacgtcgc cgaggttcat cccgttcagc gtcgagcgca ccaggccgag
     1021 atcgacggcg cggccggggg tggacatctc cagcaccgtg ccgcccttga agtcgacgcc
     1081 gcagttcagc ccgccgcacg gcggcttcgc cggatagatg gtgaggaaca gcgaggcgat
     1141 caccgccagc accgagagcg ccgccgccag ccgcgcaaag gcgacgaagc ggaggttggt
     1201 cttctcggga aggtatttga tcagcggcca acgcatcgcg ccctcactgg atcggaagct
     1261 tcttgggacg cacggccttg aaccaccagg cgatcagcac ctggctgatc agcacggcgc
     1321 tgaacagggt ggtgaacacg ccgatggtca gcgtccaggc gaagccgcgc acggggccgg
     1381 cgccgaactg gaacatgatc agggcggcca cgagggtggt gatgttggcg tccaggatgg
     1441 tcaccatcgc ccgcgcgaag ccggcgtcgg cggcgacgat cggcggcctg ccggcgcgca
     1501 cctcctcgcg catgcgctcg tagatcagca cgttggcgtc caccgccacg gcgagtgtca
     1561 ggatcagccc cgcgatcccg ggcagcgaca gagccgcccc cgtcagcgac agcgccgcga
     1621 tgatcagcag cccgttcacc agcagcgcca cgatcgagat gccgccgaac agcaggccgt
     1681 aggccagcac catgaagccg agcaccgcca cgaaggccag caccgtcgac agcttgcccg
     1741 cctcgatggc gtcctggccg agctcggccc cgacggtgct ctgctgctcg acgttgatcg
     1801 gcgccggcag ggcgccggcc tgcagtagga tggcgaggtc gcgcgccgtc tcgacggtga
     1861 agttgccgct gatctgcgcc gagc
//
LOCUS       DASVVN010000190         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_453934,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000190 DASVVN010000000
VERSION     DASVVN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453934"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VF686_02925"
     CDS             complement(<1..626)
                     /locus_tag="VF686_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660795.1"
                     /translation="MSQTDAIAPAAPPPAQAGGLGSKAWVALGALWFIYVLNFLDRQL
                     LSILAKPIQDTLHITDSQLGLLGGLYFAFFYCFIAIPVGWLADRTNRVAVLAIACAIW
                     SAATMACGLSRNFGQLAVSRMTVGFGEAGGVPPSYAIITDYFPPGRRGMALGIYNLGP
                     PIGAALGIAFGASVAAAFSWRMAFVVIGAIGLIAAAAVIFVVREPKRGG"
     gene            complement(663..>1946)
                     /locus_tag="VF686_02930"
     CDS             complement(663..>1946)
                     /locus_tag="VF686_02930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8660796.1"
                     /translation="AGSPNDERRRAVTEVGGLPFAPDPEALGFDKAGLARLDAWMADM
                     VGAGQAAGVSTLLLRHGQVVDFRTWGRARLDDEAPLARDAIFRIYSMTKPVTGAALMI
                     LFEEGRWRLDDPVTKFVPEFSDLKVYAGVDADGAMIIEDMLRPPTMRELMSHTAGFGY
                     GLFDVHPVERAYREQGVLQAESLDDLVRRAARIPLMFQPGTEWFYSIATDIQGLIVER
                     LSGQTFGTFLQERIFGPLGMVDTGFQTRPDNVHRLAAIYADDGAGGLKMATDAFDLPL
                     NDFTQAPPFEGGGGGLVSTAADYARFCQMILNRGELDGVRILTPGSVDLMGQNAIPDA
                     VLARVNPLRLLPFNPALGFGLDFAVVIDPAALGLVEGRGTLSWGGGGGTWFWIDPEND
                     LIFIGLIQRLADPVGNAFRAQARALVYAALTHPEK"
BASE COUNT          349 a          669 c          644 g          284 t
ORIGIN      
        1 ccgccccgct tgggttccct gaccacgaag atcaccgccg cggctgcgat cagaccgatc
       61 gcgccgatca ccacgaaggc catgcgccag ctgaacgccg cagcgaccga agccccgaag
      121 gcgatgccca gggccgcgcc gatgggcggt cccaggttgt agatgcccag cgccatcccg
      181 cgtcgtccgg gcgggaaata gtcggtgatg atggcgtagg acggcggcac tcctccggcc
      241 tcgccgaagc cgacggtcat gcgcgaaacc gccaactgcc cgaagttgcg tgacagacca
      301 caggccatgg tcgcagcgct ccagatggcg caggcgatgg ccagcaccgc cacccggttg
      361 gtccggtccg ccagccagcc gaccggaatg gcgatgaaac agtagaagaa cgcgaagtag
      421 agcccgccca gcagccccag ctggctgtcg gtgatgtgca gcgtgtcctg gatcggcttg
      481 gccaggatcg acagcagctg ccggtcgagg aagttcagga cgtagatgaa ccacagcgcg
      541 ccgagcgcga cccaggcctt ggagccgagg cctccggcct gcgcgggcgg cggcgcggcc
      601 ggggcgatcg catcggtctg gctcatgatc gtttcctcgg tgaagcgagc cttcgggccc
      661 gtctattttt cgggatgggt cagggcggcg tagacaaggg cgcgggcctg ggcgcggaag
      721 gcgttgccga cggggtcggc gagccgctgg atcaggccga tgaagatcag gtcgttctct
      781 ggatcgatcc agaaccaggt gcccccgcct ccgccccagc tcagggtgcc gcggccttcg
      841 acgaggccga gcgcagcggg atcgatgacg acagcgaaat ccagaccgaa ccctaacgcc
      901 ggattgaagg gcagcagccg caggggattg acccgcgcca gcaccgcatc gggaatggcg
      961 ttctgcccca tcagatcgac cgagcccggc gtaaggatgc gcacgccgtc cagttcgccc
     1021 cggttcagga tcatctggca gaagcgggcg tagtccgcgg cggtcgagac cagaccccct
     1081 ccaccgccct cgaacggcgg ggcttgagtg aaatcgttga gcggcaggtc gaaggcgtcg
     1141 gtcgccatct tcagaccgcc ggcgccgtca tcggcgtaga tggccgccag ccggtgcaca
     1201 ttgtccggac gggtctggaa gcctgtatcg accatgccga gcgggccgaa aattcgttcc
     1261 tgcaggaatg tcccgaaagt ctggcccgac agccgttcga cgatgagccc ctgaatgtcc
     1321 gtggcgatcg aatagaacca ctccgtcccc ggctggaaca tcaggggaat tcgcgcggcc
     1381 cgacggacca gatcgtcgag actttccgcc tgcagcacgc cctgttcgcg atacgcccgc
     1441 tcgaccgggt gaacgtcgaa caggccgtag ccgaagccgg ccgtatggct catcagctcg
     1501 cgcatcgtcg gcggccgcag catgtcctcg atgatcatcg cgccgtccgc gtcgacgccc
     1561 gcatagacct tcagatccga gaactccggg acgaacttcg tgaccggatc gtcgagccgc
     1621 cagcgcccct cctcgaacag gatcatcagc gctgcgccgg tcaccggctt ggtcatggaa
     1681 tagatgcgga agatcgcatc gcgagccagc ggcgcctcgt catcgagccg cgccctgccc
     1741 catgtcctga agtcgacgac ctgtccgtgc cgcagaagca gggtcgagac ccccgccgcc
     1801 tgccccgccc cgaccatgtc cgccatccag gcgtccaggc gcgcgaggcc cgccttgtcg
     1861 aagccgaggg cttcgggatc gggcgcgaac ggaaggccgc cgacctcagt caccgcccga
     1921 cgtctttcgt cgttcggcga ccctgc
//
LOCUS       DASVVN010000191         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_6327, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000191 DASVVN010000000
VERSION     DASVVN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6327"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="VF686_02935"
     CDS             complement(<1..1817)
                     /locus_tag="VF686_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX8660797.1"
                     /translation="MQLRSLRAAISATASIVAVAGVMAVVAAPSSARADEGMWTFDNF
                     PIQTVNDKYGTRIDQAWLDRVRNAAVRIQGCSASFVSDEGLILTNWHCVVGCAQELSS
                     PEQDYVKNGFLTRNREEEKRCPGQTAEVLVDIVDVTDRVLAAGTGLEGAAFNAARTAE
                     TNTIQTEACAGDPKFSCQVISFYRGGRYAMYKFRKYDDVRLVFAPENQAAFFGGDPDN
                     FNFPRYALDAGFLRAYENGQPVESPNFLKWNPNPPKEGDVTFVAGNPGTTQRLLTMSQ
                     LERLRDQQLPVTLIQNSELRGRLLEYSLTGDEAKRLSFDPIFGLENSFKVYYGQQGAL
                     TDPAFMAAKREAEAELRARVAADPALVQQIGDPWGDLERVQGVARDLYLPFRQLEQSA
                     GGGSQLYQMARTIVRASKNTTMTDAQRARLVAAVGAETPIATDMEEIRMRYWLSKTRE
                     YLTVDNPQVKAMLGRESPEALADRLISNTRMGDAAFRAQAAGMTTAQLAEADPLLAFV
                     IANDDAAKAIGDRWAAEVNAPTARAAEKVAQARFAAYGTNLYPDATFSLRLSYGQVAG
                     WTYRGVTVPPFTYMGGLYERATGAEPFNAAQAFIDNEARV"
     gene            2009..3181
                     /locus_tag="VF686_02940"
     CDS             2009..3181
                     /locus_tag="VF686_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8660798.1"
                     /translation="MSLALSTLLFEWRRYMAAVMALALSGLLVLAMTGVFIGIGKGFT
                     ATIERSSADIIIMQPGAKSLMGGPSGVPRRFIPLAYRNPAVIEVQPLDGAGGSFQSVK
                     NVDPTMSQAERARQGAPRQEFVQASIIDTTPGAVTIPTDYSQELVDALRQPYTVAIDE
                     TAVSKLGVKLGDKALYNGQTVTVVGITRGYPNMMQATIVMSRDTLRMLGQADTGARVG
                     PLMVKLRDPSQAERVAVELNEQGDGQWKAWTRQELADANASAMFEEGILVIIIGGCVV
                     LGTIIGVAITWQTLRGAIMANIKEFASLRALGVGMGSLNRIVMELSFWVGLVGVGAAI
                     GLTLLVQMLAAANAVIIALPPILLIVVGGGLVIIAMLSGLLSLGILKNSQPADLLR"
     gene            3178..>3669
                     /locus_tag="VF686_02945"
     CDS             3178..>3669
                     /locus_tag="VF686_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8660799.1"
                     /translation="MTGHTKVHGKHGDFAIEAKGLVKRFKSGKSYIDVLKGVDFDARH
                     GDLTMVMGPSGSGKSTLIAALSGLLRPEEGRVDTLDVDDLWKLSGGKIDKFRLDHCGF
                     IFQGFNLFPALSALQQVEVVLKFQGLSKGEAKRQATATLQEVGLGNRLHQRPSALSGG
                     EKQR"
BASE COUNT          578 a         1238 c         1217 g          636 t
ORIGIN      
        1 acccgggcct cgttatcgat aaaggcctgc gccgcgttga acggctccgc ccccgtggcc
       61 cgctcgtaca ggccgcccat gtaggtgaac ggcggcacgg tcacgccacg ataggtccag
      121 cccgcgacct ggccgtagga caggcgcagc gagaaggtgg cgtcgggata caggttggtg
      181 ccataggccg cgaaccgggc ctgggcgacc ttttcggcgg cgcgggcggt cggggcgttg
      241 acctcggcgg cccagcggtc gccgatcgcc ttggcggcgt cgtcattggc gatcacgaag
      301 gccagcagcg gatcggcctc ggccagttgc gccgtggtca tgccggcggc ctgggcgcgg
      361 aaggcggcgt cgcccatccg cgtgttcgag atcagccggt cggcgagggc ctcggggctt
      421 tcgcggccca gcatcgcctt cacctgcgga ttgtcgacgg tcagatactc gcgcgtcttg
      481 gacagccagt agcgcatacg gatctcttcc atatccgtcg cgatcggcgt ttcggccccc
      541 accgctgcga ccagacgcgc gcgctgggcg tcggtcatgg tcgtgttctt cgacgcccgc
      601 acgatcgtcc gggccatctg gtacagttgc gagccgccgc cggccgactg ctccagctgg
      661 cggaagggca ggtacaggtc gcgggccacg ccctggacgc gttccagatc gccccacgga
      721 tcgccgatct gctgcaccag ggccgggtcg gcggcgacgc gggcccgcag ttcggcctcg
      781 gcctcacgct tggcggccat gaaggcggga tcggtcaggg cgccttgctg gccgtaatag
      841 accttgaagc tgttctccag accgaagatg ggatcgaacg acagccgctt ggcctcgtcg
      901 ccggtcagcg aatattcgag cagacgcccg cgcagttccg agttctggat cagggtcacc
      961 ggcagctgct ggtcgcgcag ccgctccagc tggctcatgg tcagcaggcg ctgggtggtg
     1021 ccagggttgc cggccacgaa ggtcacgtcg ccttccttgg gcgggttcgg gttccacttc
     1081 aggaaattcg ggctctcgac cggctggccg ttctcgtagg cgcgcaggaa accggcatcg
     1141 agggcgtagc gcgggaagtt gaagttgtcg gggtccccac cgaagaaggc cgcctggttc
     1201 tcgggcgcga acaccagccg cacgtcgtca tacttgcgga acttgtacat ggcgtagcgg
     1261 ccgccgcggt agaagctgat cacctgacag ctgaatttcg gatcgccggc gcaggcttcg
     1321 gtctggatgg tgttggtctc cgccgtgcgg gcggcgttga aggccgcgcc ttccagtccg
     1381 gtcccggcgg ccagcacgcg gtcggtcacg tcgacgatgt cgaccagcac ctcggctgtc
     1441 tggccggggc agcgtttctc ttcctcgcgg ttgcgggtca ggaagccgtt cttcacatag
     1501 tcctgctcgg gcgaggacag ctcctgcgcg cagccgacga cgcagtgcca gttggtcagg
     1561 atcaggccct cgtcagagac gaacgaggcc gagcagccct ggatgcgcac cgcggcgttg
     1621 cggacccggt ccagccacgc ttggtcgatc cgggtgccgt atttgtcgtt caccgtctgg
     1681 atcgggaaat tgtcgaaggt ccacatgcct tcgtcggcgc gggccgaaga gggggcggcc
     1741 accacggcca tcacgccggc gacggcgaca atcgaggcgg tcgccgaaat ggcggcgcga
     1801 agcgaacgaa gctgcattct gcgaaatctc ccatgtccgg cgtcgatcgc ggactgttgc
     1861 agttacctag acccgatgaa ccgatcccgt ccatcgcctc gcggacggct cacgaaagcg
     1921 taacgtcggt cgccttaccc cgatttaact tggttccaac caatcgcgtc gtcacatagc
     1981 ggtaattgtc ggggatctgt tctaaccaat gtcgctagcc ctctccacgc tgctgtttga
     2041 gtggcggcgc tatatggccg ccgtgatggc gcttgcgctg tcgggcctgc tggtgctggc
     2101 catgaccggc gtcttcatcg gcatcggcaa gggcttcacc gccaccatcg agcgttccag
     2161 cgccgacatc atcatcatgc agcccggggc caagagcctg atgggcgggc cttcgggcgt
     2221 gccgcggcgg ttcattccgc tggcctaccg caacccggcc gtgatcgagg tccagccact
     2281 ggacggcgcc ggcggctcgt tccagtcggt caagaacgtc gacccgacca tgtcccaggc
     2341 cgagcgcgcc cgtcagggcg cccctcgtca ggagttcgtc caggccagca tcatcgacac
     2401 cacccccggg gcggtgacca tccccaccga ctattcccag gaactggtcg acgccctgcg
     2461 ccagccctac accgtcgcca tcgacgaaac cgctgtctcc aagctgggcg tcaaactggg
     2521 cgacaaggcc ctgtacaacg gccagaccgt caccgtcgtg ggcatcaccc gcggctatcc
     2581 caacatgatg caggccacca tcgtcatgtc gcgcgacacc ctgcgcatgc tcggtcaggc
     2641 cgataccggg gcccgtgtcg ggccgctcat ggtcaagctt cgcgacccgt cgcaggcgga
     2701 gcgcgtcgcg gtcgagctga acgaacaggg cgacggccaa tggaaggcct ggacccgtca
     2761 ggagctggcc gacgccaacg cctcggccat gttcgaggaa ggcattctgg tcatcatcat
     2821 cggcggctgc gtggtgctcg gcaccatcat cggcgtggcc atcacctggc agaccctgcg
     2881 cggcgccatc atggccaaca tcaaggagtt cgcctccctg cgggcgctgg gcgtcggcat
     2941 gggatcgctg aacaggatcg tcatggagct cagcttctgg gtcggcctcg tcggcgtcgg
     3001 cgccgcgatc ggcctgaccc tgctggtgca gatgctggcg gcggccaacg ccgtcatcat
     3061 cgccctgccg cccatcctgc tgatcgtcgt cggcggcggc ctcgtcatca tcgccatgct
     3121 gtcgggcctg ctgtccctcg gcatcctcaa gaacagccag cccgcggacc tgctgcgatg
     3181 accggtcaca ccaaggttca cggcaagcac ggcgacttcg ccatcgaggc caagggtctc
     3241 gtgaagcgct tcaagtcggg caagtcctac atcgacgtcc tcaagggcgt ggatttcgac
     3301 gcccgccacg gcgacctgac catggtcatg ggcccctcgg gttcgggcaa gtcgacgctg
     3361 atcgcggccc tcagcggcct gttgcggccc gaggaaggcc gcgtcgacac gctggacgtc
     3421 gatgatctgt ggaagctgtc cggcggcaag atcgacaaat tccgcctcga ccactgcggc
     3481 ttcatcttcc agggcttcaa tctgttcccc gccctgtcgg ccctgcagca ggtcgaggtg
     3541 gtgctgaaat tccagggcct gtccaagggc gaggccaagc gtcaggcgac cgcgaccctt
     3601 caggaggtcg gcctcggcaa tcggctgcac cagcggccct cggccctgtc cggcggtgag
     3661 aaacagcgc
//
LOCUS       DASVVN010000192         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_32047, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000192 DASVVN010000000
VERSION     DASVVN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32047"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VF686_02950"
     CDS             complement(<1..867)
                     /locus_tag="VF686_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8660800.1"
                     /translation="MRSTGADILVVDDEADIRELVSGLLEDEGHAVRTASNSDEALAA
                     IRARKPSLALLDIWMQGGGLDGLELLDVIKELDPDLPVVMISGHGNIETAVTALQRGA
                     YDFIEKPFKSDRLVVVVQRALETSSLKRENRRLRAQVAAPTGFIGRSAAAQALRSTIA
                     KVAPANSRVLISGPPGSGKELVARQIHEASARAKGEFVAIAAAGMTPERLDIELFGEE
                     GVDGRPRKIGVFERAHNGTLYLDDVGDMPRESQSRVLRVLVEQRFRRVGGDNDVQVDV
                     RVVSSSSKDLREE"
     gene            complement(877..>1859)
                     /locus_tag="VF686_02955"
     CDS             complement(877..>1859)
                     /locus_tag="VF686_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8660801.1"
                     /translation="PDIIGRPLGEVSPELSELAGRAEAHIEEEIDVSLEGETRRLRVR
                     IEGGVGGEMVLTFDDITRLVTAQRNAAWRDVARRIAHEIKNPLTPIQLSAERLRRRYR
                     GQVMDDVEVFDRCTETIIRQVGDIGRMVDEFSSFARMPAPRFTAADPAELLREAVFAQ
                     RVAEAEIGVELVEPLPKVTLHADRPMIGQALTNILKNAGEAVSARRAIAPRPDDGDRP
                     GISARLIVEDGMATFVIEDDGLGLPAKDRDRLTEPYVTTREKGTGLGLAIVKRICEEH
                     GGELKLADAEHLSGARVCLIFPLKSQPKSAGAAGAKDAKASRTPTLPAAE"
BASE COUNT          257 a          673 c          623 g          306 t
ORIGIN      
        1 ctcctcgcgc aggtctttcg acgacgacga gaccacgcgc acgtcgacct gcacgtcgtt
       61 gtcgcctcca acgcggcgga agcgctgctc gaccagcacg cgcagcaccc ggctctggct
      121 ctcgcgcggc atgtcgccga cgtcgtccag atagagggtg ccgttgtggg cccgttcgaa
      181 cacgccgatc ttgcgcggac ggccgtccac gccctcctcg ccgaacagtt cgatgtccag
      241 acgctcgggg gtcatgccgg cggcggcgat ggccacgaac tcgcccttgg cccgggcgct
      301 ggcttcgtgg atctggcgcg ccaccagttc cttgcccgac cccgggggac ccgagatcag
      361 gacccggctg ttggccggag ccaccttggc gatggtgctg cgcagggcct gcgccgcggc
      421 ggagcggccg atgaagccgg tcggcgccgc cacctgggcg cggagacgcc ggttctcgcg
      481 cttcagcgaa ctggtctcca gcgcgcgctg cacgaccacc accagccggt cggacttgaa
      541 cggcttttcg atgaagtcat aggccccgcg ctgcagcgcc gtgaccgcgg tctcgatgtt
      601 gccgtggccc gagatcatca ccaccggcag gtcgggatcc agctccttga tcacgtccag
      661 cagctccagt ccgtccagac cgccgccctg catccagatg tccagcagcg ccagcgacgg
      721 ctttctggcc cggatcgcgg cgagcgcctc gtcggaattg gaggccgtcc gcacggcgtg
      781 gccctcgtcc tcgagcaggc ccgagaccag ctcgcggatg tcggcctcgt cgtcgacgac
      841 cagaatgtcg gctcccgtgg atcgcatatc gcctcgctat tcggcagccg gaagggtcgg
      901 ggtccgggac gctttcgcat ccttcgcccc ggccgcgccg gccgatttcg gttgggattt
      961 gagagggaag atcaggcaca cgcgggcgcc cgacaggtgt tcggcgtcgg ccagcttcag
     1021 ctcgccgcca tgttcttcac agatgcgttt gacgatcgcg aggcccaggc ccgtgccctt
     1081 ctcgcgcgtc gtcacatagg gttcggtcag gcggtcccta tccttggcag gcaggcccag
     1141 gccgtcgtcc tcgatgacga aggtcgccat cccgtcctcg acgatcagcc gcgccgaaat
     1201 gcccggacgg tcgccgtcgt ccggccgggg cgcaatggcc cgccgcgcgg acacggcctc
     1261 gccggcgttc ttgaggatgt tggtcaaggc ctggcctatc atgggccggt cggcgtgcag
     1321 ggtgaccttg ggcaggggtt cgaccagttc caccccgatc tccgcctccg ccacccgctg
     1381 ggcgaacacc gcctcgcgca gcagttcggc cggatcggcg gcggtgaagc gcggcgcggg
     1441 catgcgggcg aaggacgaga actcgtccac catccggccg atgtcgccga cctgacggat
     1501 gatggtctcg gtgcagcggt cgaacacctc gacgtcgtcc atgacctgac cgcggtaccg
     1561 ccgccgcagc cgttcggccg acagctggat cggcgtcagc gggttcttga tctcatgcgc
     1621 gatccgccgc gccacgtcgc gccacgccgc gttgcgctgg gcggtcacca gccgggtgat
     1681 gtcgtcgaag gtcagcacca tctcgccgcc gaccccgccc tcgatgcgga cgcgcagacg
     1741 ccgcgtctcg ccctcgaggc tgacgtcgat ctcctcctcg atatgcgctt cagcccggcc
     1801 ggccagttcg ctgagctcgg gggacacctc gcccagcggg cggccgatga tgtccgggt
//
LOCUS       DASVVN010000193         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_479580,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000193 DASVVN010000000
VERSION     DASVVN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479580"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VF686_02960"
     CDS             complement(<1..933)
                     /locus_tag="VF686_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8660802.1"
                     /translation="MIKRHFFLAAAAVLLGLMVVAVVLRMAFAEDEKGGGGPGGRGGP
                     GGGRGQTVSEAVVGARSFTDEIRVLGVARGRRSVNITSSTSELITRVLFTDGQRVAAG
                     TPLVELQAREEDAGIIEARAQVAQAQRQYDRFKGLADRGIAPRVSAEEAETALATARA
                     SLTAAQARRGDRMIRAPFAGVLGLSSVTAGTLVNPGGIITTLDDIDVVRVDFPVPERY
                     LGVLRAGTPIRATIDAYGNEVFTGRIALIDTRINEQTRAVTARAEVPNPGYRIRPGMA
                     VRVAVQQGQRTAPAAPEAAVQYEGDGAFVYRIAKG"
     gene            complement(1060..1527)
                     /locus_tag="VF686_02965"
     CDS             complement(1060..1527)
                     /locus_tag="VF686_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660803.1"
                     /translation="MTWQAWRDDRTAASLYAGYADGGEGRNAGYAAPGQGDSDWELRG
                     SIGRSFDGWSGRWAPTGSFVEVQAARRMRQGLPDETRIDATAGAHFSEDWMVLAQAYG
                     GQADDGGPRWLSIEASVVRDFGDWSLQAGWRQAVAGREAPESGGPIIALWRRF"
     gene            complement(1555..1839)
                     /locus_tag="VF686_02970"
     CDS             complement(1555..1839)
                     /locus_tag="VF686_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660804.1"
                     /translation="MRLAGGLLIGLAALTAASAAHAGPWTQPKGQGQLILKYEDMHAD
                     RGFDPGGSLADLPAERRDTSLGVFAEYGLTDRFTLQFKGDWQSGEDAFVD"
     gene            complement(1850..>2348)
                     /locus_tag="VF686_02975"
     CDS             complement(1850..>2348)
                     /locus_tag="VF686_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8660805.1"
                     /translation="NPAGFTIHSAVHGAREDAHFVMHLHTDAGVGVAAQAEGLLPIGQ
                     NACLLQHQVAYHGYEGLALNHDERERLVADLGDKPLMLLRNHGTLAVGQTAAAAWVGM
                     FFLERACAQQVAALSGGRDHVLFAPDAAQAETKEQGRGIGFISSLAWPGALRMLDRKS
                     PGYDA"
BASE COUNT          324 a          819 c          863 g          342 t
ORIGIN      
        1 accctttgcg atgcgataga cgaaggcgcc gtcgccttca tattgcaccg ctgcttcagg
       61 cgcagcgggt gcggtgcgct gaccctgctg gacggcgacg cgaacggcca tgccggggcg
      121 gatgcggtag ccggggttgg ggacctccgc gcgcgcggtg acggcgcggg tctgttcgtt
      181 gatgcgggtg tcgatcaggg cgatgcggcc ggtaaagacc tcattgccat aggcgtcgat
      241 ggtggcccgg atcggcgtgc cggcgcgcag cacgcccaga taccgctcgg gcaccgggaa
      301 atcgacgcgc acgacgtcga tgtcgtcgag ggtggtgatg atgccgcccg gatttacgag
      361 ggtgccggcc gtcaccgagc tgaggcccag aacgccggcg aagggggcgc ggatcatgcg
      421 gtcgccgcgg cgcgcctggg cggccgtcag cgaggcgcgg gcggtggcga gggcggtctc
      481 ggcctcctcg gcgctgaccc ggggcgcgat gccgcggtcg gccagcccct tgaagcggtc
      541 atactggcgc tgggcctggg cgacctgcgc gcgggcctcg atgatgccgg cgtcttcctc
      601 gcgcgcctgc agctcgacca ggggcgtgcc cgccgcgacg cgctggccgt cggtgaacag
      661 gacgcgggtg atcagttcgc tcgtcgagga ggtgatattg accgaacggc ggccgcgggc
      721 gacgcccagc acccggatct cgtcagtgaa cgagcgcgcg ccgacgacgg cctcggacac
      781 cgtctggccg cgtccgccgc cgggaccccc gcggccgccg ggaccgccgc cgcccttctc
      841 gtcttccgca aaggccatcc tcaggacgac ggccacgacc atcaggccca gcaggacggc
      901 ggcggcggcg aggaagaagt ggcgtttgat cacgcgggac gctctctggc agggggaacg
      961 agccgactta gcggctgagg ccgtcgacgc aaactgaagt gtttgtaacg ggtgttgcgg
     1021 cggtttccgt cgcccggacg ccgcgcttct atgtcgcggt cagaaccgac gccagagggc
     1081 gatgatgggc cctccgcttt ccggggcctc gcgcccggcg acggcctgac gccagccggc
     1141 ctgaaggctc cagtcgccga agtcgcggac cacggaggcc tcgatcgaca gccagcgcgg
     1201 accgccgtcg tcggcctgtc cgccataggc ctgggcgagc accatccagt cctcgctgaa
     1261 atgcgcgcca gccgtggcgt cgatgcgggt ttcgtcgggc aggccctgac gcatgcggcg
     1321 cgcggcctgc acctccacga agctgccggt gggagcccag cgtccgctcc agccgtcgaa
     1381 cgagcgtccg atcgaaccgc gcagctccca gtcgctgtcg ccctgccccg gcgcggcata
     1441 gccggcgttg cggccctcgc ccccgtcggc gtagccggcg tagaggctgg cggccgtccg
     1501 gtcgtcgcgc catgcctgcc aggtcacgcc gatctccagc gggccgcggc cgtcctagtc
     1561 gacgaaggcg tcctcgccgg actgccaatc gcccttgaac tgcagggtga agcggtcggt
     1621 caggccgtat tcggcgaaga cgccgagcga ggtgtcgcgc cgttcggcgg gcaggtccgc
     1681 cagcgagccg ccggggtcga agccgcggtc ggcgtgcatg tcctcgtatt tcaggatcag
     1741 ctggccctgc cccttgggct gggtccacgg gcccgcgtgg gcggcgctcg ccgccgtgag
     1801 ggccgccagt ccgatcagca gccccccggc cagtctcatc gcatcgggct caagcgtcgt
     1861 agcccgggga tttccggtcc agcatccgca acgcgcccgg ccaggccagg ctggagatga
     1921 agccgatccc ccggccctgc tccttggttt cggcctgggc ggcgtcgggg gcaaacagca
     1981 catggtcgcg tccgcccgac agagccgcca cctgctgggc gcaggcgcgc tcgaggaaga
     2041 acatcccgac ccatgccgcc gcggccgtct ggccgaccgc aagcgtgccg tgattgcgca
     2101 gcagcatcag cggcttgtcg cccagatccg ccaccagccg ctcacgctcg tcgtggttca
     2161 gcgccagacc ttcgtagccg tgataggcga cctgatgctg cagcaggcag gcgttctggc
     2221 cgatcggcag caggccctcg gcctgggcgg cgacgcctac gccggcgtcg gtgtgcaggt
     2281 gcatgacgaa atgggcgtct tcgcgcgcgc catgcaccgc ggaatggatg gtgaagccgg
     2341 ccgggttg
//
LOCUS       DASVVN010000194         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_84856, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000194 DASVVN010000000
VERSION     DASVVN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84856"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VF686_02980"
     CDS             <1..655
                     /locus_tag="VF686_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX8660806.1"
                     /translation="MTVRGLILASVAVLALTASGAVVASALNALPQDAVPVQDPTAAL
                     NAQPTPPAPVEPAPQGTPAPASPVAVTPPAPAIVIAEDGLDEDEAEEEKAETPAVKRV
                     ETPAEPARRQRRRVAIVEAIDKITAESMRFEVEVGGRPVRFQKTLIFTARACEVSAAD
                     EQVSDAIAYLEVSLQPRGVVQVSEPRQIFRGWMFASAPAVNGLQHPVYDAWVVGCKA"
     gene            complement(622..1317)
                     /gene="aat"
                     /locus_tag="VF686_02985"
     CDS             complement(622..1317)
                     /gene="aat"
                     /locus_tag="VF686_02985"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX8660807.1"
                     /translation="MDEPGFSASSPFGGFGPDELLQCYATGVFPMGEGRDDPRIFLVE
                     PEQRGVIPLDRFHVPTRLKRTVRADPFEIRVDTAFDAMLDACAAPGPGREDSWINGPI
                     RRLYVELNARGRAHSIECWRDERLVGGLYGVTLGGAFFGESMFSRERDASKVALVHLV
                     ARLKRGGWRLLDAQFLTGHLSQFGAVETPQSFYLAMLKVAQGVQPDTRSLFKPMTGAE
                     AVFYALQPTTQAS"
     gene            complement(1325..2677)
                     /gene="accC"
                     /locus_tag="VF686_02990"
     CDS             complement(1325..2677)
                     /gene="accC"
                     /locus_tag="VF686_02990"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8660808.1"
                     /translation="MFTKVLIANRGEIALRIHRACKEMGISTVAVHSEADRGAMWVRL
                     ADESVCIGPAPAAKSYLNIPSIIAAAEITGAQAIHPGYGFLSENARFAEIVEAHGMTF
                     IGPKPEHIRIMGDKITAKQTVLDAGIPCVPGSKGEVETVEAAIEASKAIGFPLIVKAA
                     AGGGGRGMKVALTADDLVEAVQTAQSEAAAAFGNGAVYMERYLQKPRHIELQVIADSH
                     GNVVHLGERDCSLQRRHQKVLEEAPSPALSAAERVAIGETVNKAIAAIGYLGVGTIEF
                     LWEDGEFFFIEMNTRLQVEHPVTEMITGVDLVREQIRIAAGLPLSFRQEDVHFEGHAI
                     ECRINAENAETFTPSPGTITDFHAPGGLGVRLDSAIYAGYSIPPYYDSLIGKLIVHGR
                     DREECIARLKRSLNEMVVGGIDTTIPLFQKLLQEPDILSGDYDIHWLENWAKRQKGDA
                     "
     gene            complement(2682..>2868)
                     /locus_tag="VF686_02995"
     CDS             complement(2682..>2868)
                     /locus_tag="VF686_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8660809.1"
                     /translation="PEAPPFCQPGDQVKKGQTLLIVEAMKTMNPIQSPRDGVVKEVMV
                     GDAQPVEFGEPLVLLEA"
BASE COUNT          447 a          949 c          987 g          485 t
ORIGIN      
        1 aatgacggtc cgtggtctca ttctggccag cgtcgcggtt ctggcgctga ccgcctcggg
       61 cgctgtcgtc gcctcggctc tgaatgccct gccgcaggac gccgtgccag tgcaggaccc
      121 gaccgccgcc ttgaacgccc agccgacgcc gccggctccg gtcgagcctg ccccgcaagg
      181 cacgcccgcc ccggcctcgc ccgttgcggt gacgccccca gccccggcca tcgtcatcgc
      241 cgaagacggt ctcgacgagg atgaggctga ggaagaaaag gccgaaacgc cggcggtcaa
      301 gcgcgtcgaa acgccggccg agccggcccg ccgccaacgc cggcgcgtcg ctatcgttga
      361 agccatcgac aagatcaccg ctgaatccat gcggttcgag gtcgaggtcg gcggccgccc
      421 ggtgcgcttc cagaagaccc tgatcttcac cgcccgcgcc tgcgaggtgt ccgcggccga
      481 cgagcaggtg tccgacgcca tcgcctatct ggaagtctcg ctacagcccc gcggcgtggt
      541 tcaggtcagc gagccgcggc aaatcttccg cggctggatg ttcgcctcgg cgcccgccgt
      601 caacggcctg cagcacccgg tctatgacgc ctgggtcgtg ggctgcaagg cgtagaagac
      661 cgcctcggcg cccgtcatcg gcttgaaaag cgaccgcgta tcgggttgta cgccctgcgc
      721 gaccttcaac atcgcaagat agaaactctg cggggtttcc acggctccaa attggctcag
      781 atgcccggtc aggaactggg cgtccagcaa acgccatccg ccgcgcttca gccgggcgac
      841 cagatggacc agcgccacct ttgacgcgtc cctttcccgg ctgaacatgc tctcgccgaa
      901 gaaggccccg cccagcgtca cgccatacag gccgccgacc agccgttcgt cgcgccagca
      961 ttcgatggaa tgcgcccggc cgcgggcgtt gagctcgacg tacaggcggc ggatcgggcc
     1021 gttgatccag ctgtcctcgc gccccggacc cggcgccgcg caggcgtcga gcatggcgtc
     1081 gaaggccgtg tcgacccgaa tttcgaacgg gtcagcccgg accgtgcgct tcagccgggt
     1141 cgggacgtga aaccggtcca gcgggatgac gccgcgctgc tcgggctcga cgaggaagat
     1201 gcgcgggtcg tcgcggcctt cgcccatagg gaagacgccg gtggcgtagc actgcagcag
     1261 ttcgtcaggg ccgaagccgc cgaagggact gctggcgctg aagcccggct cgtccatcac
     1321 gccgtcaggc gtcgcccttc tggcgtttgg cccagttctc cagccagtgg atgtcgtagt
     1381 ccccggacag gatgtcgggc tcctgcagca gtttctggaa caggggaatg gtggtgtcga
     1441 tgccgccgac gaccatctcg ttcaggctgc gcttgaggcg ggcgatgcac tcctcgcggt
     1501 cgcggccgtg gacgatcagc ttgccgatca ggctgtcgta atagggcggg atcgaatagc
     1561 cggcgtagat ggcgctatcc agccgcacgc ccagaccgcc gggggcgtgg aagtcggtga
     1621 tggtgccggg cgagggggtg aaggtctcgg cgttctcggc gttgatccgg cactcgatgg
     1681 cgtggccttc gaaatggacg tcctcctgcc tgaacgacag cggcagaccg gcggcgatgc
     1741 gaatctgctc gcgcaccaga tcgacgccag tgatcatttc cgtaaccgga tgctcgactt
     1801 gcaggcgggt gttcatctcg atgaagaaga actcgccgtc ttcccacagg aattcgatcg
     1861 tgccgacgcc gagatagccg atggcggcga tggccttgtt gacggtctcg ccgatggcga
     1921 cgcgctcggc cgcggacagg gcgggcgagg gggcttcttc cagcactttc tggtggcgac
     1981 gctgcagcga gcagtcgcgt tcgccgaggt ggacaacgtt gccgtggctg tcagcgatga
     2041 cctgaagctc gatatggcgc ggcttctgga gatagcgctc catgtagacg gcgccgttgc
     2101 cgaaggccgc cgccgcctcg gactgggcgg tctggacggc ctcgaccaga tcatcggcgg
     2161 tcagggccac cttcatgccg cgcccgccgc cgccggcggc ggccttgacg atcaggggga
     2221 agccgatggc cttggaggct tcgatggcgg cctcgacggt ttcgacctcg cccttcgagc
     2281 ccggcacgca ggggatgccc gcatccagca ccgtctgttt ggcggtgatc ttgtcgccca
     2341 tgatgcggat gtgctcgggc tttggcccga tgaaggtcat gccgtgggct tcgacgatct
     2401 cggcgaagcg ggcgttctcg gacaggaagc cgtagccggg gtggatcgcc tgggcgccgg
     2461 tgatctcggc cgccgcgatg atcgaaggga tgttcagata ggatttggcc gccggagcgg
     2521 ggccgatgca gacgctttcg tccgccagcc gcacccacat ggcgccgcgg tcggcttcgg
     2581 agtgcacggc gacggtggag atgcccatct ccttgcaggc gcggtggatc cgcagggcga
     2641 tttcgccgcg gttggcgatc aggaccttcg tgaacatggg cttacgcctc gagcaggacg
     2701 aggggctcgc cgaactcgac cggctgggcg tcgccgacca tgacttcctt cacgacgccg
     2761 tcgcgcgggg actgaatcgg gttcatcgtc ttcatggcct cgacgatcaa cagggtctga
     2821 cccttcttga cctggtcgcc cggctggcaa aacggcggcg cctcgggc
//
LOCUS       DASVVN010000195         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_506499,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000195 DASVVN010000000
VERSION     DASVVN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506499"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF686_03000"
     CDS             <1..187
                     /locus_tag="VF686_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660810.1"
                     /translation="WCETRDDFRTFRTDRVVEADFLDDRYPGRPAVLRGKWYAKVAAE
                     HAAWEAAGRPMPPGVSQ"
     gene            complement(270..1475)
                     /locus_tag="VF686_03005"
     CDS             complement(270..1475)
                     /locus_tag="VF686_03005"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8660811.1"
                     /translation="MSREAVIVSTARTPIGRAYRGAFNDTQAQQLGAHAVRHAVARAG
                     VDPAEIEDVIMGAALQQGSTGTNVARQVALAAGLPVTTAGMSLDRQCASGLMGIATAA
                     KQVIFDHQKVVIGGGLESISLVQNQNMNLYRGKDEDLLKLAPHIYMSMLETAEVVSRR
                     YNISRDAQDEYALQSQQRTAQAQAEGRQDAEIVPMTSTMLVVDKATGETSRKEVTLSK
                     DEGNRADTTLEGLKSLKPVFGSGEEIQQGEFITAGNASQLSDGASACVIMEAGEAVRH
                     GLQPLGAYRGMAVVGCEPDEMGIGPVFAVPKLLKRHGLTVDDIGIWELNEAFAVQVLY
                     CRDRLGIPNERLNVSGGAISIGHPYGMSGARMTGHVLIEGKRRGAKYGVVTMCVGGGM
                     GAAGLFEIY"
BASE COUNT          228 a          585 c          574 g          303 t
ORIGIN      
        1 ctggtgcgaa acccgcgacg acttccgcac gttccgcacc gaccgggtgg tcgaggccga
       61 tttcctcgac gaccgctatc ccggccgtcc cgccgtgctg cgcggcaagt ggtatgcgaa
      121 ggtcgccgcc gagcacgccg cctgggaggc ggcgggccga ccgatgccgc ccggcgtttc
      181 ccagtagatc tcgaacaggc cggccgcgcc gcaaccgaac cgcatcgcgg ttcggtcact
      241 ttctctttgg gcgcagcttt cggccgttct cagtagattt cgaacaggcc ggccgcgccc
      301 atgcctccgc cgacgcacat ggtgacgacg ccgtatttcg cgccgcgccg cttgccttcg
      361 atcagcacat ggccagtcat ccgggcgccc gacatgccgt aggggtggcc gatcgagatg
      421 gcgccgcccg agacattgag ccgctcgttg ggaatgccca gccggtcgcg gcaatagagc
      481 acctgcacgg cgaaggcctc gttcagctcc cagatgccga tgtcgtcgac cgtcagcccg
      541 tggcgcttca gcagtttggg tacggcgaag accgggccga tgcccatttc gtcgggctcg
      601 cagccgacca cggccatgcc gcgataggcg cccagcggct gcaggccgtg acgaaccgcc
      661 tcgcccgcct ccatgatgac gcaggccgag gccccgtccg acagctggct ggcgttgccg
      721 gcggtgatga actcgccctg ctggatctcc tcgcccgagc cgaacaccgg cttcagggat
      781 ttcagaccct cgagcgtcgt gtcggcgcgg ttgccctcgt ccttcgacag ggtcacctcc
      841 ttcctcgagg tctcgccggt cgccttgtcg acgaccagca tggtcgaggt catcggcacg
      901 atctcggcgt cctgccggcc ctcggcctgc gcctgggccg tccgttgttg ggactgcaaa
      961 gcgtattcgt cctgggcgtc gcggctgatg ttgtagcggc gcgacacgac ctctgccgtc
     1021 tccagcatcg acatatagat gtgcggcgcc agcttcagca ggtcctcgtc cttgccgcgg
     1081 tacaggttca tgttctggtt ctgcaccagc gagatcgatt ccagcccgcc gccgatgacg
     1141 accttctgat ggtcgaagat gacctgtttg gcggccgtgg cgatgcccat caggcctgag
     1201 gcgcactggc gatccaggct catgccggcg gtggtcacag gcaggccggc ggccagggcg
     1261 acctgacgcg cgacgttcgt gccggtcgag ccctgctgca gggccgcgcc catgatcacg
     1321 tcctcgattt cggccggatc gacgccggcc cgggccacgg cgtgacgcac cgcatgggcg
     1381 cccagttgct gcgcctgggt gtcgttgaag gcgccccggt aggcgcggcc gatgggggtg
     1441 cgggcggtgg agacgatgac ggcttcgcgg gacatccggg gtggtccttc cttgtggccc
     1501 taccgctcgc ctcgcggagg ctcgctgctt gagggcggat gggtgcgcta tcgctcgcct
     1561 ctgcggaggc tcgccgcttg agcgcgggtt gtgacggacc ctaggcgctt gccgtgaggt
     1621 caaggcaagg gggcggcggg tcgatcagcc ctgcgccgcc tgcgccttgg ccagttcgat
     1681 catcccctgc
//
LOCUS       DASVVN010000196         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_348716,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000196 DASVVN010000000
VERSION     DASVVN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348716"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..340
                     /locus_tag="VF686_03010"
     CDS             47..340
                     /locus_tag="VF686_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660812.1"
                     /translation="MLNRRGALGLAALAAGPKPPGLTPQAVAPPDPQEVIRLWPGAAP
                     GGANVTVTPRVVERSTDPAFHDRFAQYTTDPILTVLRPERPNGASLLLIPGGG"
     gene            350..1021
                     /locus_tag="VF686_03015"
     CDS             350..1021
                     /locus_tag="VF686_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8660813.1"
                     /translation="MVDKEGLDCARPFAAAGVTCFILRYRQPADGWAAGPAAPLQDAQ
                     RAMRLMRQRAVADGLDPGRAAVLGASAGGHLAGALTVGRDAVYEAVDEADALPFRPDL
                     SILMYPVATMEGPFVHAGSRTHLLGPDPSAEAVARWSLERMDWTGAPPTFLLHAMDDD
                     SVPVENSLQLLGAIRAAGVKAEAHLFEEGGHGFGVRLIAGKPASVWPQLVLAWAARRG
                     WVAPA"
     gene            1211..1549
                     /locus_tag="VF686_03020"
     CDS             1211..1549
                     /locus_tag="VF686_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8660814.1"
                     /translation="MREDGKLDYLEMPGGELDAVKAFYGAAFGWTFQDYGPSYAAFDQ
                     GLDGGFDADAAPDAKPLPVLFAHDLEAMAAKVGAAGGRIVKPIFAFPGGRRFHFRDPA
                     GNELAVWSET"
     gene            complement(1546..>1790)
                     /locus_tag="VF686_03025"
     CDS             complement(1546..>1790)
                     /locus_tag="VF686_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660815.1"
                     /translation="FVMVYAGPASQFPIYSGQMVEAGGRASVVSTEDGVRHAQEHLFQ
                     RPDAPREIHVWTMSLDGADRTIAEAIAQSVDLRTGL"
BASE COUNT          262 a          595 c          651 g          282 t
ORIGIN      
        1 ctgacaccgg tttcctgata gctttcggct acgggagaca cggatgttgc tgaaccgaag
       61 aggggcgctg gggttggccg ccctggccgc cgggccgaaa ccgccgggcc tgacgccgca
      121 ggccgtggcg cccccggacc cgcaggaggt catccgtctg tggcccggcg cggcgcccgg
      181 cggagcgaat gtgacggtga cgccccgggt ggtggaacgc tcgaccgatc cagccttcca
      241 cgaccgcttc gcccagtaca ccaccgaccc gattctgaca gtgctgaggc ccgagcggcc
      301 gaacggagcc tccctgctgc taatcccggg cggcggctag cgatgggcgg tggtggacaa
      361 ggagggtctg gactgcgcgc gtccgttcgc ggcggcgggg gtcacctgtt tcatcctgcg
      421 ctatcgccag ccggccgatg gctgggccgc cgggcccgcc gccccgctgc aggacgcgca
      481 gcgggccatg cggctgatgc gccagcgggc ggtggcggac gggctggacc ccggacgcgc
      541 agccgtactc ggagcctccg cgggcgggca ccttgccgga gcactgaccg tgggcaggga
      601 cgccgtctat gaggcggtcg acgaggccga cgccctgccg ttcaggcccg acctgtcgat
      661 cctgatgtat ccggtggcga ccatggaggg gccgttcgtt catgccgggt cacgcacaca
      721 cctgctaggc cccgatccgt cggcggaagc cgtcgcccgc tggtcgctgg agcggatgga
      781 ctggaccggc gcgccgccga ctttcctgct ccacgcgatg gacgatgact cggtgccggt
      841 cgaaaacagc cttcagctgc tgggcgccat ccgcgccgcc ggggtgaagg cagaggcgca
      901 tctgttcgag gaaggcggcc acggcttcgg cgttcggctg atcgccggaa agcccgcctc
      961 ggtctggcct cagctggttc tggcctgggc cgcgcggcga ggctgggtcg cgccggcctg
     1021 accatctccg cccgcctgcc ttgccgctgg tcccgggcgg cgctcgctgg ccatcaggcg
     1081 gacaaggccg gtctggccga caccgagcac gatcatcgca tggctcctgt gttgcgaatg
     1141 attctcagat ttgcccggaa gtcggcctgt ggcaagccga aagcggatgg ctttcaaacg
     1201 gagcaataag atgcgtgaag acggaaagct ggattatctg gaaatgccgg gcggcgagct
     1261 ggacgcggtg aaggccttct acggcgcggc cttcggttgg acgttccaag actatggccc
     1321 gagctatgcg gcgttcgatc agggactgga cggcgggttc gacgccgacg ccgcgccgga
     1381 cgccaaaccc ctgcccgtcc tgttcgcgca tgatctggag gcgatggcgg ccaaggtcgg
     1441 ggcggcgggc gggcgtatcg tcaagccgat cttcgccttt cccggcgggc ggcggttcca
     1501 cttccgggat ccggccggca acgaactggc ggtctggtcg gagacctaga ggcccgtccg
     1561 cagatcgact gactgggcga tcgcctccgc aatcgtccgg tcagcgccgt ccagactcat
     1621 ggtccagacg tggatctcgc gcggggcgtc cggccgctgg aacaggtgtt cctgggcgtg
     1681 acgcacgccg tcctcagttg agaccaccga ggcgcgtccg ccggcctcga ccatttggcc
     1741 gctgtagatc gggaactgcg aggcaggacc ggcgtagacc atgacgaacg
//
LOCUS       DASVVN010000197         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_506642,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000197 DASVVN010000000
VERSION     DASVVN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506642"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..360)
                     /locus_tag="VF686_03030"
     CDS             complement(4..360)
                     /locus_tag="VF686_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8660816.1"
                     /translation="MTQLRGVFLTSGDPSRTARFYEQVAQLPIERVGDEGGYQYWKMD
                     HNGMQLAIHASEPFSSYTHPPLAGSNLTHLYFRIEDHAGFLDHLGRLALTPFAVDDVV
                     ITVEDPDGRKVMFGVA"
     gene            complement(469..915)
                     /locus_tag="VF686_03035"
     CDS             complement(469..915)
                     /locus_tag="VF686_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660817.1"
                     /translation="MATALNVSRGSFYWHFRDVADFRSQILQRWADKTTDDVRRELEA
                     DAAAPERLGSLMKRALGTKHPLDRAIRAWAADDAAVAGIVASVDARRAGYITELLLAA
                     GVDDRLARRRAAFLYWALLGQAFVMDPDQSALAASGMVEIGDLFKT"
     gene            1078..1473
                     /locus_tag="VF686_03040"
     CDS             1078..1473
                     /locus_tag="VF686_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660818.1"
                     /translation="MTWQNAALGLAGFIGIGVAVIHGVLVHRLMVKPLKALFLVDDRL
                     QPPLRRLTAPLLQFSTFNWFIGGLALIAAANGSEPDVRLATGLLVGSSYLFGAVCNLW
                     STRRPHPGWILYALALVLIGLGLSVGAIE"
     gene            1808..>2072
                     /locus_tag="VF686_03045"
     CDS             1808..>2072
                     /locus_tag="VF686_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8660819.1"
                     /translation="MNRRGLLAAGAALALPWTPGDDARDGWTPDQFHAARQFVDLPGG
                     RVAFVELGSGPAAIFLHGYPLNGFQWRGAMARLSPHRRCVAPDL"
BASE COUNT          319 a          714 c          689 g          350 t
ORIGIN      
        1 cggtcaggcg acgccgaaca tcaccttgcg gccgtcggga tcttcgaccg tgatgaccac
       61 gtcgtcgacc gcgaagggcg tcaacgccaa ccggcccaga tggtcgagaa accccgcgtg
      121 gtcctcgatc ctgaaataca gatgcgtcag gttcgacccg gccagcggcg gatgcgtgta
      181 gctcgagaac ggctccgaag catggatcgc aagctgcatg ccgttgtgat ccatcttcca
      241 gtattgatag ccgccctcgt ctcccacgcg ttcgatcggc agttgcgcaa cctgctcgta
      301 gaagcgggcg gttcgggaag ggtccccgga cgtcaggaaa actccacgta gctgtgtcat
      361 cagcggcctc cggcttgtgg ttgggaaaac tggcgagttc gtcggccgtc cgcaaggcgc
      421 ccggaccggc cggcgggttg cgggaaggcg ccaagtttcg gccgacgctc atgttttgaa
      481 cagatcgccg atctcgacca tgcccgacgc ggcgagggcg gactggtccg gatccatgac
      541 gaaggcctgg cccaggagcg cccagtacag gaacgccgcg cggcgccgcg ccagccggtc
      601 gtcgacaccc gcggcgagca gcagctcggt gatgtagccg gcccgtctgg cgtcgacgga
      661 ggccacgatc ccggcgaccg cggcgtcgtc ggcggcccag gccctgatgg cgcggtccag
      721 gggatgcttc gttccaagag cgcgcttcat gagcgagccc agacgctcgg gcgcagcggc
      781 gtccgcctcg agctcgcgcc ggacgtcgtc ggtggtcttg tcggcccagc gttgcagaat
      841 ctgcgatctg aagtccgcga cgtcgcggaa gtgccagtaa aagctccccc gcgacacgtt
      901 cagggcagtc gccatgggac ccgccttcag ggcgttggga ccctcgtggg ccagggtccg
      961 cagtccgtgc ctcagccagt catccttcgt caggcgctcg ttcatgaccc caccatacag
     1021 cagtgtattg acctgccaag atcgaccgcc tatctatcca tacagaggtg tatggttatg
     1081 acctggcaga acgcggccct ggggctcgcc ggattcatcg gcatcggcgt cgcggtgatc
     1141 catggggtcc tcgtccaccg gctcatggtg aagccgctga aggcgctctt cctcgtcgat
     1201 gatcgcctgc aacccccgct ccgacggctc acggccccgc tcctgcagtt cagcaccttt
     1261 aactggttca tcggcggact ggccctgatc gccgcggcga acgggtcgga gccggacgtg
     1321 cgcctcgcga cgggcctgct ggtcggcagc tcctatctgt tcggcgccgt gtgcaatctg
     1381 tggagcacgc ggcggccgca tcccggctgg attctctatg cgctcgcgct cgtcctcatc
     1441 ggcctggggc tctctgtcgg cgcgatcgaa tgagcgagcg gatcggcgtg cgtcgcggcc
     1501 cgatggcgat cgtgcataca cgttagtctt ggctgatagg ttagtgtatg ctaatgcgtt
     1561 cgcatgaccg gcccccgccg ccaacgccgt cacatccgcc gccgatcctg ttcgcgccct
     1621 ctgcccccgg ggctggtcgc ggggccggcg cgatcccctt catgcggccg acggcgcgcg
     1681 acgtgaccgg gccgcaggtc gcccatgcgg ccggcgacct ggcccgacga cctgacgaca
     1741 aaggagacga cttcaaatga acaggaccac agcgagagaa gaagcgccgt cgccggccgg
     1801 acctccgatg aaccgccgcg gactcctggc ggccggcgcc gccctcgcgc tgccgtggac
     1861 gcctggcgac gacgcccggg acggctggac ccccgaccaa ttccacgccg cgcgccaatt
     1921 cgtggatctg cccggaggcc gggtcgcgtt cgtcgagctc ggaagcgggc ccgcggccat
     1981 cttcctgcac ggctatccgc tgaatggctt ccagtggcgc ggggcgatgg cccgcctttc
     2041 gccgcatcgt cggtgcgtcg cgccggacct ga
//
LOCUS       DASVVN010000198         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_243373,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000198 DASVVN010000000
VERSION     DASVVN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243373"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VF686_03050"
     CDS             complement(<1..768)
                     /locus_tag="VF686_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX8660820.1"
                     /translation="MSVLPLKQALAGVMSLTLASPMAPPQSADRPVDRDKPSDGVVSA
                     AACAPFGFTLRDDRPIPPPRYVPPPVTRPIGPTTKEDRVEYQGPPVVMPGPPPPPPPP
                     PPPPPAAMVSAQGQVSMNEVAITGSRIQSGGHQIAPRPADTERYPDATPNPVRRVADE
                     PVSTFSIDVDTASYSNVRRFIDDGRAPPADAVRVEEMINSFDYGYARPTSATQPFAIT
                     TAVAASPWSPGRQIVHVGLQGYELPASERRPLNLTFLV"
     gene            922..>2040
                     /locus_tag="VF686_03055"
     CDS             922..>2040
                     /locus_tag="VF686_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX8660821.1"
                     /translation="MHETTSMGLGHAVALLAAMVVAATLFRRFGLGSVLDYLAAGLLI
                     GPSVLGVVRDPQSVLHVAEFGVVMFLFIIGLEMRPARLWSLRKEIFGLGAVQVLACAI
                     LLTGIALLAGLPWYAAVIAGFGFALSSTAIVMQLLEERNENAEPGGQRVVSILLFEDL
                     AIVPLLALIAVLAGTYGSAVETGNPIWMTIGLAVAAVALVYVAGRWVVNPFFRLLARY
                     GGREVMTMGALLVVLGAAWAMNLGGLSMAMGAFLAGVLLSESSFRHQLEADVEPFRGL
                     LLGLFFLSVGMSLDLRVVVADWAWVLGGLAAFMTAKMAAVYSVARLKQKHGEGLLRAA
                     LMGEGGEFAFVLYSTAFAAGLFDARTAAILSAVVILSMA"
BASE COUNT          249 a          691 c          740 g          360 t
ORIGIN      
        1 gacgaggaag gtcaggttca gcggccgccg ctcgctggcg ggcagttcat agccctgcag
       61 ccccacatgc acgatctgcc ggcccgggga ccagggcgag gccgcgaccg ccgtggtgat
      121 ggcgaacggc tgggtggccg aggtcgggcg ggcgtagcca tagtcgaaag aattgatcat
      181 ctcctcgacc cgcaccgcgt cggcgggcgg cgcgcggccg tcgtcgatga agcgccgaac
      241 gttcgaatag ctggctgtgt cgacgtcgat cgagaaggtc gagaccggct cgtcggccac
      301 gcgccggacc ggattgggcg tggcgtcggg atagcgttcg gtgtcggctg gccggggcgc
      361 gatctggtgc ccgcccgatt ggatgcgcga accggtgatt gcgacctcgt tcatcgaaac
      421 ctgcccctgc gcactgacca tcgcggcggg tggcggcgga ggaggcggtg gcggcggcgg
      481 gggcggtccg ggcataacga cgggaggccc ctgatattcg acgcggtctt ccttggtggt
      541 cggtccgatc ggccgggtca cgggcggcgg gacgtagcgc ggcggcggga tcggacgatc
      601 gtcgcgcaag gtgaagccga acggcgcgca ggccgccgcc gaaaccacgc cgtccgacgg
      661 cttgtcccgg tcgaccggcc ggtcggcgga ctggggcggc gccatcggcg acgccagcgt
      721 caggctcatc accccggcga gggcctgttt cagcggaagg acggacatcg gcgcatctcc
      781 cttgggttgt cagatccgca tcaggccgac ccgtctccgg cgaaactgtg gcggtttcag
      841 cgagccgtgt tcgacagccg cggggtggcg caaacgcgca tcacccccca ctatccagct
      901 tcgcgataca gggggatccg catgcacgag acgaccagca tgggcctggg ccacgcggtg
      961 gcccttctgg ccgccatggt cgtggccgcc accctgttcc gccgcttcgg cctcgggtcg
     1021 gtgctcgact atctggcggc gggcctgctg atcgggccgt cggttcttgg cgtggtgcgc
     1081 gatccccagt cggtgctgca cgtggccgag ttcggcgtcg tcatgttcct gttcatcatc
     1141 ggactggaga tgcggcccgc ccgcctgtgg agcctgcgca aggagatctt cggccttggc
     1201 gcggttcagg tcctggcctg cgccatcctg ctgacgggca tcgccttgct ggccggcctg
     1261 ccctggtacg cggccgtcat cgccggcttc ggcttcgccc tgtcgtcaac ggccatcgtc
     1321 atgcagctgc tggaagagcg caacgagaac gccgagccgg gcgggcagcg ggtggtctcc
     1381 atcctgctgt tcgaagattt ggccatcgtg ccgctgctgg ccctgatcgc ggttctggcc
     1441 gggacctacg gctcggcggt cgagaccggc aaccccatct ggatgaccat cggcctggcc
     1501 gtcgccgccg tggccctggt ctacgtcgcc gggcgctggg tggtgaaccc cttcttccgc
     1561 ctgctggccc gctacggcgg ccgcgaggtc atgaccatgg gcgcgcttct ggtcgtcctg
     1621 ggcgcggcct gggccatgaa cctcggcggc ctgtcgatgg ccatgggcgc cttcctggct
     1681 ggcgtcctgc tgtctgaatc ctcgttccgc caccagttgg aggccgacgt cgagcccttc
     1741 cgcggcctgc tgctgggcct gttcttcctc agcgtcggca tgtcgctgga cctgcgcgtc
     1801 gtcgtcgccg actgggcgtg ggtgctgggc gggctggcgg ccttcatgac cgccaagatg
     1861 gccgccgtct attcggtcgc gcggctgaag cagaagcacg gcgagggcct gctgcgcgcc
     1921 gccctgatgg gcgagggggg cgagttcgcc ttcgtcctct actccaccgc cttcgccgcc
     1981 ggcctgttcg acgcccgcac cgccgccatc ctctccgccg tggtcatcct gtcgatggcc
//
LOCUS       DASVVN010000199         4457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_454209,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000199 DASVVN010000000
VERSION     DASVVN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454209"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VF686_03060"
     CDS             <1..617
                     /locus_tag="VF686_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX8660822.1"
                     /translation="APPVLRALLGLHVRTTFNSVTVDGDTSTNDTCLLFATGSSGAPR
                     IGRVGDRRLQEFSAALDKVMLDLAHQLVRDGEGATKFVRVTVDGAASPASARKLAKSI
                     ADSPLVKTALAGEDANWGRVVMAVGKTEEPVDRDRLGIRFGPHVAAVDGAPSPTYDEA
                     KMSAYMKNAEIDIIVDVGSGRASATVWTCDLTKRYIEINGDYRS"
     gene            614..1024
                     /locus_tag="VF686_03065"
     CDS             614..1024
                     /locus_tag="VF686_03065"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX8660823.1"
                     /translation="MSEGLPTVLVVAVALIDADGRVLIAQRPEGKQLAGLWEFPGGKV
                     EPGERPEAALIRELKEELGIDVREACLAPFVFTSHAYESFHLLMPLFLCRRWSGVVQN
                     REHAALKWVRPNQLSDWPMPPADEPLVAWLRDLL"
     gene            1047..1226
                     /locus_tag="VF686_03070"
     CDS             1047..1226
                     /locus_tag="VF686_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8660824.1"
                     /translation="MLRFLSRLRRDDSGATAIEYGLIISLIFLVILGALQAFGATGSG
                     VFNGAMDKLRAAMGG"
     gene            complement(1223..2107)
                     /locus_tag="VF686_03075"
     CDS             complement(1223..2107)
                     /locus_tag="VF686_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8660825.1"
                     /translation="MSASSGPPRIFDVPRRTARLARSEPAIGRADFLHVRAADNAVGS
                     LEALLRDWPNVLDLSAHPGAFAAALKGSAAEGRVGPVVELGDRAARAAPGAAALNVPD
                     QSQDLIVSLMSLHWANDLPGALAQIRRALKPDGLFIGTLLGAGTLKELRGVLTEAELE
                     ARGGAQARVSPFADGFDGAALLQRTGFALPVADVDRLTVRYPDLFALVRDLRAMGETN
                     VLAGPLRPLTRGIAGRAAELYTGRFGETDGRIPATFEIVHLAGWAPHESQQKPLPRGS
                     AKMRLADALGVKEQTGED"
     gene            2106..2912
                     /locus_tag="VF686_03080"
     CDS             2106..2912
                     /locus_tag="VF686_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX8660826.1"
                     /translation="MRLQDGGWRDRLKAGGQQITRGARSLGRGLTDLLLPPLAWDSPE
                     AAASAGLTPDAWSRVVFLEDPVCDGCGAGFEMDGGAFASPRCAACLAQPYAFDRARAA
                     CVYDAASRGLILKFKHGDHQPFAPLFARWVSRSAAPLLEQADAVAPVPLHRLRLLSRR
                     FNQAAEIARPLARDAGLDYLPDALVRETHTTSQGGKSARGRRLNVGKAFSVTADGRRR
                     VKGRRILLVDDVLTTGATAEACARALLEAGARAVDLAVVARVRTARELPI"
     gene            2938..3195
                     /gene="grxC"
                     /locus_tag="VF686_03085"
     CDS             2938..3195
                     /gene="grxC"
                     /locus_tag="VF686_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX8660827.1"
                     /translation="MADVVIYTKPGCGYCHAAKDLLNEKGADFTEIVASNDPAKKQEM
                     IQRSGGRMTFPQIFIDGKHIGGSDDLHALDRKGALDALLEA"
     gene            3192..4031
                     /locus_tag="VF686_03090"
     CDS             3192..4031
                     /locus_tag="VF686_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8660828.1"
                     /translation="MMATLPIALIQTRTPDSPAAAFAHAEPLIREAAAGGAKLILTPE
                     ASNFMIRDKDRRAAALTDQDHDEAVVALRGLARELGVWLLIGSAIVRSGHDGDDRAAN
                     RSLLIDDRGRIVSTYDKLHVFDVDLPTGERWRESATVRPGDEAVVVDTPWGRLGLTIC
                     YDIRFPHLYRALAKAGAAMIAVPAAFTVPTGEAHWETMLRARAIETGCWILAPAQAGT
                     HDDGRRTWGRSTVVAPWGEVTAKLDHDEPGVLFATLDFDAVDRARAAIPQLTHDRAFA
                     GPR"
     gene            4028..>4457
                     /locus_tag="VF686_03095"
     CDS             4028..>4457
                     /locus_tag="VF686_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX8660829.1"
                     /translation="MIRYALQCEHDHGFEAWFSASADYDDQAARGLVECPLCGSHSVS
                     KQIMSPAVAGARKRETAPDQAKMQTMILQAAREARAHVEQNFDYVGDAFAREARDIHE
                     GRSEKREIYGEATPAEVKKLKDDGVPCAPLPPLPPDPSKAH"
BASE COUNT          676 a         1669 c         1445 g          667 t
ORIGIN      
        1 tcgccccgcc ggtgctgcgc gccctgctgg gcctgcacgt acgcaccacc ttcaacagcg
       61 tcacggtcga cggcgacacc tcgaccaatg acacctgcct gctgttcgcc accggctcgt
      121 cgggcgcgcc gcgtatcggc cgggtcggcg atcgccggct gcaggagttc tcggccgccc
      181 tcgacaaggt catgctggac ctggcccacc agctggttcg cgatggcgag ggcgcgacga
      241 aattcgtccg cgtcaccgtc gacggcgccg ccagtcccgc ctcggcccgc aagctggcca
      301 agtcgatcgc cgacagccct ctggtgaaaa ccgccctcgc cggcgaggac gccaactggg
      361 gccgggtggt catggccgtc ggcaagaccg aggagccggt cgaccgcgac cgcctcggaa
      421 tccgcttcgg cccccacgtc gccgccgtcg acggcgcgcc ttccccgacc tacgacgagg
      481 cgaagatgag cgcctatatg aagaacgccg agattgacat catcgttgac gtcggttcgg
      541 gccgcgcctc ggccaccgtc tggacctgtg atctcaccaa acgctacatc gagatcaacg
      601 gcgactatcg ctcgtgagtg agggacttcc taccgtcctc gtcgtcgccg tggccctgat
      661 cgacgccgac ggccgcgtgc tgatcgccca gcggcccgag ggcaaacagc tggccggcct
      721 gtgggaattc cccggcggca aggtcgaacc gggcgaacgg cccgaggccg ccctgatccg
      781 cgagctgaag gaagagctcg ggatagatgt aagagaggca tgcttggcgc cgttcgtgtt
      841 taccagccac gcttacgaat cctttcacct gttgatgcca ctgttcctgt gccgacgctg
      901 gtcgggggtg gttcagaacc gcgagcatgc ggcgctgaaa tgggtgcggc cgaaccagct
      961 ttcggactgg cccatgcctc cggccgatga acctctggtc gcctggctgc gcgacctgct
     1021 ctagccgcca agggaggggg ccgatgatgc tacgcttcct ttcccgcctc cggcgcgacg
     1081 acagcggtgc gaccgccatc gagtacggcc tgatcatttc gctgattttc ctcgtcatcc
     1141 tcggcgcgct tcaggcgttc ggcgcgacgg gcagcggcgt cttcaatggc gccatggaca
     1201 agctgcgcgc ggcaatgggc ggctagtcct ctccggtctg ctccttcacg ccaagcgcat
     1261 ccgccagccg catcttcgcc gaacccctcg gcagcggctt ctgctgactc tcatgcgggg
     1321 cccagccggc cagatggacg atctcgaacg tcgccggaat ccgcccgtcg gtctcgccga
     1381 accgcccggt gtagagttcc gcggcccttc ccgcgatgcc gcgcgtcaga ggccgcaatg
     1441 gtcccgccag cacgtttgtc tcgcccatgg cccgaaggtc gcgtaccagg gcgaacaggt
     1501 ccgggtagcg caccgtcagc cggtccacat ccgccaccgg cagggcgaac ccggtccttt
     1561 gcagcagggc cgctccgtcg aagccatcgg cgaagggcga cacccgcgcc tgcgccccgc
     1621 cgcgcgcctc cagctccgcc tcggtcagca cgccgcgcaa ctccttcagc gtccccgcgc
     1681 cgagcagcgt gccgatgaac agcccgtccg gcttcagcgc ccgtcggatc tgcgccagcg
     1741 cgccgggcag atcgttggcc cagtgcaggc tcatcagcga gacgatcaga tcctgcgact
     1801 gatcgggaac gttcagcgcc gccgcgccgg gggccgcccg cgccgcccga tcgcccagct
     1861 ccaccaccgg tccgacccgc ccctcggccg cgctgccctt cagcgccgcc gcgaacgcgc
     1921 ccggatgggc cgacaggtcc agcacattcg gccagtcgcg cagcagggcc tccagactgc
     1981 ccacggcgtt gtccgccgcc cggacgtgca ggaagtcggc gcgcccgatc gcaggctcgc
     2041 tacgcgcaag ccttgccgta cggcgtggaa cgtcgaagat gcggggggga ccggaggagg
     2101 cgctcatgag gctgcaggat gggggctggc gcgaccgatt gaaagcgggt ggccaacaga
     2161 tcacgcgcgg cgcgcggtca ctggggcgag gcctgaccga ccttctgctg cccccgctcg
     2221 cttgggacag ccccgaggcc gcggcctcgg cgggcctgac gcccgacgcc tggagccgcg
     2281 tcgtcttcct cgaggatccg gtctgcgacg gctgcggcgc gggcttcgag atggacggcg
     2341 gcgccttcgc ctcgccgcgc tgcgccgcct gcctggccca gccctacgcc ttcgaccggg
     2401 cgcgggcggc ctgcgtctat gacgccgcct cgcgcggtct gatcctcaaa ttcaagcacg
     2461 gcgaccacca gccgttcgcc cccctgttcg cccgctgggt cagccgctcg gccgcgcccc
     2521 tgctggaaca ggccgacgcc gtcgcccccg tgcccctgca ccgcctgcgt ctgctgtcgc
     2581 gccgcttcaa ccaggccgcc gagatcgccc gccccctcgc gcgcgacgcc ggcctcgact
     2641 atctgcccga cgccctggtc cgcgagaccc acacgacgtc tcagggcggc aaatccgccc
     2701 gcggccgccg cctgaacgtc ggcaaggcct tctccgtcac cgccgacggc cgtcgccggg
     2761 tcaagggccg ccgcatcctt ctggtcgatg acgtgttgac gaccggagcc accgccgagg
     2821 cctgcgcccg cgccctgctc gaggccgggg cgcgcgccgt cgatcttgcg gtcgtggccc
     2881 gggtgcgaac cgcccgcgaa ttgcctatct aggcggaacc gttttcagga gacctccttg
     2941 gccgacgtcg tcatctacac caagcccggt tgcggctact gccacgccgc caaggacctg
     3001 ctgaacgaga agggcgccga tttcaccgag atcgtcgcct ccaatgaccc ggcgaagaag
     3061 caggagatga tccagcgctc gggcggacgg atgaccttcc cgcagatttt catcgacgga
     3121 aaacacatcg gcggttccga cgatctgcat gcccttgacc gcaaaggcgc gctggacgca
     3181 ttgctcgagg cctgatggcc actcttccga tcgccctgat ccagacccgc accccggact
     3241 ccccggcagc ggccttcgcc catgccgaac cgctgatccg cgaggccgcg gcgggcgggg
     3301 cgaaactgat cctgacgccg gaagcgtcca acttcatgat ccgggacaag gaccggcgcg
     3361 ccgccgccct gacggatcag gaccacgacg aggccgtcgt cgccctgcgg ggtctggccc
     3421 gcgaactggg cgtctggctg ctgatcggtt cggccatcgt ccgctcgggt catgacggcg
     3481 acgaccgcgc cgccaaccgg tcgctgctga tcgacgaccg gggccggatc gtctcgacct
     3541 acgacaagtt gcacgtcttc gacgtcgacc tgcccaccgg cgagcgctgg cgcgagagcg
     3601 ccacggtccg tccgggcgat gaggccgtcg tcgtcgacac gccgtggggc aggctgggcc
     3661 tgaccatctg ctacgacatc cgcttccccc atctgtatcg agcgctcgcg aaagccgggg
     3721 ccgccatgat cgccgtgccc gccgccttca ccgttcctac gggcgaagcg cattgggaaa
     3781 ccatgctgcg agcgcgggcc atcgagaccg gctgctggat cctggcgccc gcacaggccg
     3841 gaacccacga cgacggccgc cgcacttggg gccgctcgac cgtcgtcgcg ccttggggcg
     3901 aggtgacggc gaaactggat cacgacgagc ccggcgtgct gttcgcgacg ctcgatttcg
     3961 acgccgtcga ccgcgctcgc gccgccattc cccagctgac ccacgaccgc gcgtttgcgg
     4021 ggccccgatg atccgctacg ccctccagtg cgagcacgac catggcttcg aagcctggtt
     4081 cagcgcctcc gccgactatg acgatcaggc cgcgcgcggt ctggtcgaat gcccgctgtg
     4141 cggttcgcat tcggtctcca aacaaatcat gtcccccgcc gtcgcgggcg cgaggaaacg
     4201 cgaaacggcg ccagatcagg cgaagatgca gaccatgatc ctgcaggccg cacgcgaggc
     4261 gcgggcccat gtcgagcaga atttcgacta tgtcggcgac gccttcgccc gcgaggcgcg
     4321 cgacatccac gagggccgct ccgagaagcg cgagatctac ggcgaggcca cgcccgccga
     4381 ggtgaagaag ctgaaggacg acggcgtccc ctgcgcgccc ctgccgccgt tgccacccga
     4441 tccgtccaag gcgcact
//
LOCUS       DASVVN010000200         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_139329,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000200 DASVVN010000000
VERSION     DASVVN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139329"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="tolB"
                     /locus_tag="VF686_03100"
     CDS             complement(<1..1106)
                     /gene="tolB"
                     /locus_tag="VF686_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX8660830.1"
                     /translation="MRLNRLLASVTALAMTASAVVAQTPPAPQQSGDPVEVVIDQGVL
                     RPYQIAVVNFAGENGSDLGNVIRADLRRSGYFEPMDPASFVETGLTLANAPNFPQWTS
                     IGAQAVLYGAVTRRPDGRNDFGFRLYDPYRQCQLASYQFTATPEQWRRIGHKIADLIY
                     QRMTGETGLFDSRVVFVSESGTQLNRLSRLAISDQDGFNPVYLTQGDEIIMSPRFSLS
                     DPNEITYVALGKDYSRIYLYNLSTGRRESLGEFDGQVLAPRFSNDGSKMAFSIIRGGN
                     TDVYVMDLSSRQTRRLTTDPGIDTSPSFSPDNSQIVFTSDRSGSAKLYVMRADGTGQR
                     AISRGGGLYTAPHWSPRGDLVAFTNQGGGRFSTGV"
     gene            complement(1219..1971)
                     /locus_tag="VF686_03105"
     CDS             complement(1219..1971)
                     /locus_tag="VF686_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660831.1"
                     /translation="MHAGAIVAVLAAGLLAPRADPRQIVNAVPVSIVSSTVIEAAAAD
                     NPSEELVTEDAATAPVAPPPEPVPTPPEPRPVPPPLPTPTPVKKAPPPRPTPPRPTPP
                     RAQPTPPRPTPPRREESLDLDALAGPPRPANRPGTRPATGQQGRGTAPRATGPAQWSA
                     IFNQVYRNWSPPCDTPGVEDMRIQMDVTITPEGRIARAPTLIGRRSDPVWIAVAAGAE
                     RALYQSAPFDVPDGFEGGTARTTFLTSRICGG"
     gene            complement(2015..2461)
                     /locus_tag="VF686_03110"
     CDS             complement(2015..2461)
                     /locus_tag="VF686_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEX8660832.1"
                     /translation="MSPLAGEDGGEFRPLAEINVTPLVDVMLVLLIIFMISAPLLTTG
                     VEVELPKTEASAVETNQEPVVVSIDRDGAIFVADSEVPWDRLILAVAQEGGEGARDKP
                     VFIRADGRAPYQAVARVMARLSGAGFTKMNLITDTSAQSSGSGGEG"
BASE COUNT          387 a          792 c          898 g          385 t
ORIGIN      
        1 acccccgtcg agaaccggcc gcctccctgg ttggtgaatg ccaccagatc gccgcgcggg
       61 ctccagtgcg gggccgtgta caggccgccg ccgcgcgaga tggcgcgctg gcccgttccg
      121 tcggcgcgca tgacgtagag cttggccgag cccgaccggt ccgaggtgaa gacgatctgg
      181 ctgttgtcgg ggctgaacga aggcgaggtg tcgatgccgg gatcggtcgt cagacgccgc
      241 gtctgacggc tggacaggtc catcacatag acgtcggtgt tgccgccgcg gatgatcgag
      301 aaggccatct tcgacccatc gttcgagaag cgcggggcca gcacctgacc gtcgaactcg
      361 cccaggctct cccggcggcc ggtcgacagg ttgtagagat aaatccggct gtagtccttg
      421 cccagcgcca cataggtgat ctcgttgggg tcgctgagcg agaagcgcgg cgacatgatg
      481 atctcgtcgc cctgcgtcag atagacgggg ttgaacccgt cctgatccga gatggcgagg
      541 cgcgacaggc ggttcagctg ggtgccgctt tccgagacga agaccacgcg gctgtcgaac
      601 aggccagttt cgccggtcat gcgctgatag atcaggtcgg cgatcttgtg gccgatgcgg
      661 cgccattgct cgggcgtggc ggtgaactgg tacgaggcca gctggcactg acgatagggg
      721 tcgtacaggc ggaagccgaa gtcgttgcgg ccgtcaggcc ggcgggtgac ggcgccgtac
      781 agcaccgcct gcgccccgat cgaggtccac tgcgggaagt tgggggcgtt ggcgagcgtc
      841 aggcccgtct cgacgaagct ggccgggtcc atcggctcga aatagcccga gcggcgcagg
      901 tcggcgcgga tgacgttgcc gaggtcggag ccgttctcgc cggcgaagtt gacgacggcg
      961 atctgatagg gccgcaggac gccctgatcg atcaccactt cgactggatc gcctgactgc
     1021 tgcggggccg gaggcgtctg ggccacaacg gcgctcgccg tcatcgccag agcggtcacg
     1081 ctggccagaa gtcggttcag tcgcatcagg ggctcccgca agttcaaact tcgtcggtct
     1141 ttatcgtcag accgttcatg gaaagccagc atggccgtga ttggggcgac tttgcggaca
     1201 gcaaagttcc cataccgcct aaccgccgca aatacggctg gtgaggaagg tcgtgcgggc
     1261 cgttccacct tcgaacccgt ccggaacgtc gaagggagcc gactgataga gcgcccgctc
     1321 cgcgcctgcc gcaaccgcga tccagacggg gtcggaccgg cgaccgatca gggtcggcgc
     1381 ccgggcgatt cgtccctccg gcgtgatggt cacatccatc tgaatccgca tgtcctcgac
     1441 cccgggtgtg tcgcagggcg ggctccagtt gcggtagacc tgattaaaga tggcgctcca
     1501 ctgggcgggg ccggtcgcgc ggggcgcggt tccccgtccc tgctgccccg tggcagggcg
     1561 tgtgcccggc cggttcgccg gacggggcgg gcctgccaag gcgtcgaggt ccagcgactc
     1621 ctcccggcgc ggcggtgtcg ggcggggcgg agtcggctgt gcgcggggcg gcgtaggtcg
     1681 gggcggcgtc ggacgaggcg gaggggcctt cttgaccggc gtgggcgtcg gaagcggcgg
     1741 cggaacgggc cgtggttcgg gcggcgtcgg aacgggctcg ggcgggggcg cgaccggcgc
     1801 ggtcgcggcg tcctcggtga ccagttcctc gctcggattg tccgcggccg cggcctcgat
     1861 gacagtggag gagacgatgg acaccgggac tgcgttgacg atctgacgcg ggtcggcacg
     1921 gggtgcgagc aggcccgccg ccagcacggc gacgatagca cccgcgtgca cgaggacgga
     1981 accgatcaga gccgggccgg gccgttccaa ggcgtcagcc ctccccgcct gatccggaag
     2041 attgggcaga ggtgtcggtg atcaggttca tcttggtgaa gccggcgccc gacagccgcg
     2101 ccatcacccg cgccaccgcc tgatagggcg cacggccgtc ggcgcgaatg aagaccggct
     2161 tgtcccgcgc gccctccccg ccttcctgcg cgacggccag gatcagacgg tcccacggga
     2221 cctcgctgtc ggcgacgaag atggcgccgt cccggtcgat cgacacgacc accggctcct
     2281 gattggtctc caccgcgctg gcctcggtct tgggcagttc gacctcgacc ccggtcgtca
     2341 gcaggggcgc cgagatcatg aagatgatca gcagcaccag catgacgtcg accagcggcg
     2401 tcacgttgat ctcggccaag ggacgaaact cgccgccatc ctccccggcc aacggcgaca
     2461 ag
//
LOCUS       DASVVN010000201         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_479955,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000201 DASVVN010000000
VERSION     DASVVN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479955"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..767
                     /locus_tag="VF686_03115"
     CDS             3..767
                     /locus_tag="VF686_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8660833.1"
                     /translation="MAGFRDIKERDLTGRQGLFVAEGEVVLNVLTSPRSLCRPVALLL
                     DAKRVAALAGVVARVTAGAPVYVADQAILDTIAGFHLHRGILALGRKPEPATLEAVLA
                     PLGADAVVVAACGIGNHDNMGGLFRNAAAFGAGAVLLDQTCCDPFYRKAIRVSVGAVL
                     RTPMAAGLDASAMIDGLERAGFSTLALTPGAETPLHRMPRGGRVALILGPEGPGLSPD
                     VIARCRPVGIPMSGGFDSLNVSVASGIALHHFTRQA"
     gene            complement(997..1070)
                     /locus_tag="VF686_03120"
     tRNA            complement(997..1070)
                     /locus_tag="VF686_03120"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Cys,seq:gca)
     gene            1211..1840
                     /locus_tag="VF686_03125"
     CDS             1211..1840
                     /locus_tag="VF686_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX8660834.1"
                     /translation="MDFAAARKVMVDSQVRVNDVTDRALQAALLAVPRERFLPEDRAW
                     SAYAEIEPEIVGGRYLMMARDLSKLLMALDARAGETALAIAAPYGAAVLARLGLTVAA
                     QEADEAVFGVTGPVLAEEGVRTVVAPLSAPSGQDYDVIISEAAVPGRPEAWLNALKIG
                     GRMAVVERGGPVGKAVLYVRGEQGVSRRELFDSTPPVLSDLAPEPAFAL"
BASE COUNT          252 a          622 c          666 g          319 t
ORIGIN      
        1 gcatcgcggg cttccgcgac atcaaggaac gtgacctcac gggcaggcag ggcctgttcg
       61 tggccgaggg cgaggtggtg ctgaacgtgc tgacctcgcc ccggtcgctg tgccggcccg
      121 tcgcgctttt gctggatgcg aaacgggtcg cggccttggc cggggtggtc gctcgcgtaa
      181 ccgccggcgc gccggtctat gtcgccgacc aggcgattct cgacaccatc gccggtttcc
      241 acctgcaccg cgggattctg gcgctggggc ggaaacccga accggcgacg ctggaggcgg
      301 tgttggcgcc gcttggagcg gacgccgtcg tggtcgcggc ctgcgggatc ggcaaccacg
      361 acaacatggg cgggctgttc cgcaacgccg ccgccttcgg ggccggcgcg gtgctgctgg
      421 accagacctg ctgcgacccc ttctaccgca aggccatccg ggtctcggtg ggggccgtgc
      481 tgcgcacgcc gatggcggcg gggctggacg cctcggcgat gatcgacggc ctggagcggg
      541 cgggtttctc caccctggcc ctgaccccgg gcgccgagac gccgctgcac cgcatgccgc
      601 gcggcgggcg ggtggcgctg attctgggtc cggaaggccc cggcctgtcg ccggacgtca
      661 tcgcccgctg ccgaccggtc ggaattccga tgtcgggcgg cttcgattcc ctcaacgttt
      721 cggtcgccag tggaatcgcg ctgcatcatt tcacccgaca ggcgtgaaac gatcacggaa
      781 ttgcgattaa cgcttcgtac accttgatga acggtcgagc ttgtgtcgct gtgaggcgtc
      841 cggggagggt ttcatcatgt ttggcggcgt tttcgtcttc atccaccgca atgcgcgcct
      901 cgcgctggcg gtgaccatcg tcacggccgg tgtcagcgcg gccacagtga tcggctggat
      961 caccgcgccg gtctgactgc ctgaagaaaa tcgcgatgga ggcctgaccg agaatcgaac
     1021 tcgggtgcaa ggatttgcag tcctctgcgt aaccactccg ccatcaggcc ctgggccgtc
     1081 gtaagacgac ctcgccatcg cgaggggcgt tcgctatcag atcggattgt cgcccgcaac
     1141 gcacagacct ataagcgcgc cagatttccc ggcgtgtcgc cgggcgccca cagtcgcagg
     1201 atcgtcgccc atggatttcg ccgccgcccg caaggtcatg gtcgacagcc aggtccgcgt
     1261 gaacgacgtc accgaccggg cgctgcaggc tgcgttgctg gccgtgccgc gcgagcggtt
     1321 cctgccggag gaccgcgcct ggtccgccta tgccgagatc gagcccgaga tcgtcggcgg
     1381 acgctacctc atgatggccc gcgacctgtc caagctgctg atggcgctgg acgccagggc
     1441 gggcgagacc gccctggcca tcgccgcccc ctatggcgcg gcggtgctgg cgcgccttgg
     1501 cctgaccgtg gctgctcagg aggcggacga ggcggtgttc ggcgtgacgg gtccggtcct
     1561 ggcggaggag ggcgtccgga ccgtggtcgc cccgctgtcg gcgccgtccg gtcaggacta
     1621 cgacgtgatc atctccgaag ccgcggttcc cggccggccc gaggcctggc tgaacgcgct
     1681 gaagatcggc ggccggatgg ctgttgtcga gcgcgggggc ccggtcggca aggccgtgct
     1741 atacgttcgc ggcgagcagg gcgtgtcgcg tcgcgagctg ttcgattcca ccccgccggt
     1801 cctgtcggat ctggctccgg agcccgcctt cgccctgtag cgggagagcg acggaatcc
//
LOCUS       DASVVN010000202         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_506856,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000202 DASVVN010000000
VERSION     DASVVN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506856"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VF686_03130"
     CDS             <1..1084
                     /locus_tag="VF686_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX8660835.1"
                     /translation="AAAAALGGFADAAAAQSRIKDIASVEGVRSNQLVGYGLVVGLNG
                     TGDSLRNCPFTRQSLEGMTERLGVNIRGANANSKNMAAIMVTAELPPFATPGARIDVS
                     VASMCDAKSLLGGTLLVTSLQGADGQVYAVAQGSIQTGAVSGSGGSGSSVTRGVPTAG
                     RIASGATVERETGFQLANMNEVRLTLRNPDFTTAQRVAAAINQSYPNTALAENGTVIA
                     LRAPGQMGMAGFISMVENMPVQVDAPAKVIIDEVNGVIVMGEAVRVSTVAIAQGNLTI
                     SVQENPQVSQPEPFSRGGQTVVVPSSTVAVEEELGRQMRNVGGGTSLSTLVNGLNALG
                     VSPRDMISILQAIKAAGALQADIEVM"
     gene            1087..1398
                     /locus_tag="VF686_03135"
     CDS             1087..1398
                     /locus_tag="VF686_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEX8660836.1"
                     /translation="MTDFPVDPALIRPLAATATGAVTDKMRKTAEAFEASFLSQMMKP
                     MFEGLSTDGMFGGGEAEGTWRGFLVEAMAKQTVKAGGIGLADQVVAQMLKMQEAQEPA
                     A"
BASE COUNT          265 a          578 c          506 g          220 t
ORIGIN      
        1 cgccgccgcc gccgctctcg gcggcttcgc ggacgccgcg gccgcccagt cccgaatcaa
       61 ggacatcgcc tcggtcgagg gagtgcgctc caaccagctg gtgggctacg gcctcgtcgt
      121 cgggctgaac ggcacgggcg acagcctgcg caactgtccg ttcacccggc agtcgctgga
      181 gggcatgacg gagcgtctcg gcgtcaacat ccgcggcgcc aacgccaact cgaagaacat
      241 ggcggccatc atggtcaccg ccgagctccc gccgttcgcc acgccgggcg cgcgcatcga
      301 cgtcagcgtg gcgagcatgt gcgacgccaa gagcctgctt ggcggcacgc tgctggtcac
      361 cagcctgcag ggcgccgacg gtcaggtcta tgcggtcgcc caaggctcga tccagaccgg
      421 cgctgtgtcg ggatcaggcg gctccggctc atccgtgacc cgcggcgttc ccacggccgg
      481 gcgcatcgcc tcgggcgcca ccgtcgagcg tgagacgggg tttcagctgg ccaacatgaa
      541 cgaggtgcgg ctgacgctgc gcaatccgga cttcaccacg gcccagcggg tcgccgccgc
      601 catcaaccag tcctacccca acacggcgct ggccgagaac ggcaccgtca tcgccctgcg
      661 ggcgccgggc cagatgggca tggccggctt catcagcatg gtcgagaaca tgccggtcca
      721 ggtcgacgcc cccgccaagg tgatcatcga tgaggtcaac ggcgtcatcg tcatgggcga
      781 ggccgtccgc gtctccacgg tcgccatcgc ccagggcaat ctgaccatct ccgttcagga
      841 gaacccgcag gtcagccagc ccgagccttt cagccgcggc ggccagaccg tcgtcgtccc
      901 ctcctccacc gtggcggtcg aggaagagct gggccgccag atgcgcaacg tcggcggcgg
      961 gacatccctg tccaccctcg tcaacggcct gaacgccctc ggcgtcagcc cgcgcgacat
     1021 gatcagcatt ctgcaggcca tcaaggccgc cggcgcgctg caagccgaca tcgaggtgat
     1081 gtgagcatga ccgacttccc tgtcgacccc gccctgatcc ggcctctcgc ggccaccgcg
     1141 acgggcgccg tgaccgacaa gatgcgcaag accgccgagg cgttcgaggc gtccttcctg
     1201 tcgcagatga tgaagccgat gttcgaggga ctctccaccg acggcatgtt tggcggcggc
     1261 gaggccgagg gcacatggcg cggcttcctc gtcgaggcca tggccaaaca gaccgtcaag
     1321 gccggcggca tcggcctggc cgatcaggtc gtcgcccaga tgcttaagat gcaggaagcc
     1381 caggaacccg ccgcatgacc gagtccgcca ccgtcaacgc caaggcccgt gtccgtcagc
     1441 tgaccgacct gaccacccgc ctcaccgaac gcctcgtcga ggaatcgaag gctttcgaag
     1501 cccgtcgccc gcaggacgcg gctgcgaccc tggccgagac ccagcaactg gccaatgtct
     1561 accgccgcg
//
LOCUS       DASVVN010000203         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_59694, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000203 DASVVN010000000
VERSION     DASVVN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59694"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..573)
                     /locus_tag="VF686_03140"
     CDS             complement(52..573)
                     /locus_tag="VF686_03140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8660837.1"
                     /translation="MSIPERLILSWVESPIGPLALACDEAGVVRGVSFGDGLIKAMRR
                     EYPDAVLSDGEAPAAVARELAAYFEGDCEALNRVPWSLEGAASGEGFQPKVWRALAEV
                     PAGVTISYGEMAKRAGEPGAAQAAGVALNRNPIPLILACHRVVGADGSLTGFGGGLER
                     KGWLLRHEGALLI"
     gene            complement(570..1355)
                     /locus_tag="VF686_03145"
     CDS             complement(570..1355)
                     /locus_tag="VF686_03145"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX8660838.1"
                     /translation="MTAFRDLHRSGLLILPNAWDGGSAALMRAKGAKAIATTSAGVAW
                     ALGWPDGDALPVDRVVQVTRDVVRAAQGLPVSIDFEGGYSDDPAEVAAQAKRLVEAGA
                     AGINIEDGGGSPELLASKIAAIRAAAGPDLFINARCDVWLRGLAKDAPVAEAARRGDL
                     YAAAGADGLFTPGLTDEADIAAMVGKTALPLNLLAFPGLPDARTLEGLGVRRLSAGSG
                     IVGATWGRAAALTEAFLIDGRSEPLGEGALGWGEVNALMPAAG"
     gene            complement(1683..>1972)
                     /locus_tag="VF686_03150"
     CDS             complement(1683..>1972)
                     /locus_tag="VF686_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660839.1"
                     /translation="FTWDWDWQASQEIEDVDTIGYGNFDRYETLDYLETGDFSQHDFS
                     VRWNMREDTIVRAGVVNAFDAEPARWLGNTTADNFDLFGRRFFLSVNYRPF"
BASE COUNT          288 a          764 c          627 g          293 t
ORIGIN      
        1 ccttgtaacc tgcaggttac atgtcacctc agggttacac gagtcaagag gtcagatgag
       61 cagcgccccc tcgtgccgca gcagccagcc cttgcgctcc agcccgccgc cgaagccggt
      121 cagcgagccg tcggcgccga ccacgcggtg gcaggctagg atcagcggga tcgggttgcg
      181 gttcagggcc acgcccgccg cctgcgccgc gcccggttcg cccgcgcgtt tcgccatctc
      241 gccatagctg atggtcacgc ccgccgggac ctcggccagc gccctccaca ccttcggctg
      301 gaagccctcg ccggacgccg cgccctccag cgaccacggc acccggttca gcgcctcgca
      361 gtccccttcg aaataggccg cgagctcgcg cgccacggcc gccggcgcct cgccgtccga
      421 caggaccgcg tccggatact cacgccgcat cgccttgatc agtccgtctc caaagctgac
      481 gccgcgcacc acgcccgcct cgtcgcaggc cagcgccaga ggcccgatcg ggctttcgac
      541 ccaggacagg atcaggcgct ccggtatgct catcccgccg ccggcatcag ggcgttcacc
      601 tcgccccagc ccaaggcgcc ttcgcccagc ggctcggacc gtccatcgat caggaaggcc
      661 tcggtcaggg ccgccgcccg gccccaggtc gcgcccacga tgccggatcc ggcgctcagc
      721 cggcgcacgc ccagcccctc aagcgtcctc gcatccggca ggccggggaa ggccagcagg
      781 ttcagcggca gggccgtctt cccgaccatc gccgcgatgt ccgcctcgtc cgtcagcccc
      841 ggcgtaaaca ggccgtccgc tcccgccgcc gcataaagat ccccgcgccg cgcggcctcg
      901 gccacggggg cgtccttcgc cagacctctc agccacacgt cgcagcgggc gttgatgaac
      961 aggtctggtc ctgcggccgc gcggatcgcc gcgatcttgc tcgccagcag ctccggcgag
     1021 ccgccgccgt cctcgatgtt gatccccgcc gcgcccgcct cgaccagccg cttcgcctgc
     1081 gccgcaacct cggccgggtc gtcggaatag ccgccctcga agtcgatcga caccggcagt
     1141 ccctgcgccg cccgcaccac gtcgcgtgtg acctgcacca cccggtccac cggcagggcg
     1201 tccccgtcgg gccatcccag cgcccaggcc acgcccgcgc tggtcgtggc gatcgccttc
     1261 gcccccttgg cccgcatcag ggccgccgaa cctccgtccc aggcgttggg caggatcagc
     1321 aggccggaac ggtgcaggtc gcggaaggcg gtcatggcgt atctccttgg aagtccggaa
     1381 catcgctccg atcccgtcgt cttgtccggc ggttttcgga cgttctcacc ggaggaacgg
     1441 aagtctccgg gttttggcgg cgatctccca actttgccga ggaccgctgc agggacaacc
     1501 acgcagtccg cgctccgcgg atatggcttt ccgcggcggc gagacgtcca gatcaagttg
     1561 aagaggtgtg acgcggcgtg tcaggcgatc ggtcccgcgc atcggccgcg cggcaatgcg
     1621 gagagcgcat cgggccgaca tcccgcgggc gacgctgccg cccgcgggag tccgtcgtgg
     1681 ccctagaacg gccggtagtt cacgctcagg aagaagcgcc ggccgaacag gtcgaaattg
     1741 tccgccgtcg tgttgcccag ccagcgggcc ggctcggcgt cgaacgcgtt gaccacgccg
     1801 gcccgcacga tggtgtcctc gcgcatgttc cagcgcaccg agaagtcgtg ctgcgagaag
     1861 tcgccggtct caaggtagtc cagcgtctcg taacggtcga agttgccgta gccgatcgta
     1921 tcgacgtcct cgatttcctg gctggcctgc cagtcccagt cccaggtgaa gg
//
LOCUS       DASVVN010000204         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_85421, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000204 DASVVN010000000
VERSION     DASVVN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85421"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VF686_03155"
     CDS             complement(<1..600)
                     /locus_tag="VF686_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8660840.1"
                     /translation="MTTPPIDRIRNFSVVAHIDHGKSTLSDRLIQFTGGLTAREMSAQ
                     VLDSMDIEKERGITIKAQTVRLNYKGKDGLDYVLNLMDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYQAIDNNHEIVPVLNKIDLPAAEPERVKSQIEDVIGIDA
                     SEAIPISAKTGIGIELVLEAVVKRLPPPRGELNAPLKALL"
     gene            845..1981
                     /locus_tag="VF686_03160"
     CDS             845..1981
                     /locus_tag="VF686_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8660841.1"
                     /translation="MRAGAAIVLVSLALAACGEGDAKKAEAKAGEKAGVKAGGGASTQ
                     TVSVAIASLQNLPRTVTASGTISAWEEVPVGAETGGLTATGVYVDEGTYVRQGQPLVQ
                     MNDALLRAQLRQQQAAVETAQANAARDQAALGRAQELKERGFLSQASLDTALANQRAS
                     SANVAAAQASLSETRTRLSQATIMAPVSGLVISRNVTRGQIISAGTELFRIVRDGRLE
                     LDAQVPETDLALIRSGQSAVISSDQIGETTGRVRIVTPEVNAENRLGVARISLVGGGF
                     RPGMFARARIQVGDQPAVTVPTASVLYRENRAGVFMLGTDNRAHFQPVTVLSRTTDST
                     AVSGLAAGARVVVAGAGFLGEGDRVTIAPAARPAPVRAAAPAKK"
     gene            1992..>4585
                     /locus_tag="VF686_03165"
     CDS             1992..>4585
                     /locus_tag="VF686_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8660842.1"
                     /translation="MKNISSWSIKNPIPIILMFVLLTLAGVNGFMGMRINNNPDIDFP
                     LINVFAGRPGAAPAEMEVPVTRLIEDSLAGLPGVRHITSQVTDGVSSTQIEFELGTDT
                     ERATNDVRNAMSGLRADLPQDMQEPSVERIDITGDALITWVVRSSTMTPEEISWFIDN
                     EVSRALLSISGVGEVNRSGGVDREIRVELDPDRLASFGLTAAQVSQALTSVNNDLPGG
                     RVTISGSERSIRTLGAAGSIEQLRETLIPLAGGRSARLGDLGTVTDHWSEPRRLARYN
                     GQEAVTFNFLRSRSASEVKVAEKVRERVAEIDEAHPELTIEKVTSSVEYIEESYIASI
                     EALLLGAGLAVIVVFIFLRDWRATFITATAMPLSLIPAFAILGPLDQSLNVVTLLALS
                     LTVGILVDDAIVEIENIVRHMRDGKSPYHASMEAADEIGLAVVATTATILAVFAPVGF
                     MPGIIGQFFKAFALAACVSVAFSLVVARTLTPLMAAYLLKHHKHEDADPFWMGTYLKS
                     LRWSLANRWKVFALGTVLFVGCGFLATKVPFEFSPSGDQSRAAFSIELPPGATLRQTD
                     AIVQQVTRDLRQRPEVTSVYASVGGQDVNQANVYADMVGKGERALSQQEFAREMVDGW
                     KAIPGARIGAGTGNQGGGPSDGTSYTFAILSDNPAALQAAARQIEAEMRTVPGLANVV
                     NTASIARPEILVTPRPDQAALMGVSTGDISQTIRVATIGDVAQNLPKYNLGDRQVPIR
                     LQLTEAAREDLSVLETLRVPTDDGGAVPLSAVADIQFGSGPSQVLRQDRSRIASITAE
                     LDGIRSGEADAAVKKLPTVQNLPAGVRQVPAGDAEFIQEMITGFAVAFMTGILLMYAV
                     LVLLFRSFA"
BASE COUNT          736 a         1573 c         1526 g          750 t
ORIGIN      
        1 cagcagcgcc ttcaggggag cattgagttc gcccctgggc ggcggcaggc gcttgaccac
       61 cgcttcgagc accagctcga tgccgatgcc ggtcttggcc gaaatcggga tcgcctcgga
      121 tgcatcgatg ccgatcacat cctcgatctg gctcttgacc cgttcgggtt cggcggcggg
      181 caggtcgatc ttgttgagga cggggacgat ctcgtggttg ttgtcgatgg cctgatagac
      241 gttggccaag gtctgggctt ccacgccctg agaggcgtcg acgaccagca gcgatccttc
      301 gcatgcggcc aatgaacgcg acacctcata ggcgaagtcc acgtgccccg gcgtgtccat
      361 cagattgagg acatagtcga gcccgtcctt ccccttgtag ttcaggcgca ctgtttgggc
      421 cttgatggtg attccccgtt ccttctcgat atccatcgaa tccagaacct gagccgacat
      481 ttcacgtgcg gtgagaccgc cagtgaattg aatcaggcgg tcggacaggg tcgatttgcc
      541 gtgatcaatg tgggccacca cactgaaatt ccggatacgg tcgatgggag gggtcgtcat
      601 ggccgcgccg gtagcacggc ggggcgcaat cgccaacgcg gaccgcgacg ccgcccttcc
      661 cggcaaccgc gattgtggcg gattacttcg ccgtaagggg gtgctttggg gtttgagaag
      721 cgccgccgga acagctattc aacggctgat gacagagcgc ccgggcggtg ccgacggtcg
      781 gcgcgcggtc cccccggctg cggcgcccag caggggatca gacgtggcga acatcaagcg
      841 gaacatgcgt gccggtgcgg cgatcgttct tgtcagcctg gccttggcgg cctgcggcga
      901 aggcgacgcc aagaaggccg aggccaaggc gggcgaaaag gccggcgtca aggccggcgg
      961 cggcgcgtcc acccagaccg tctcggtcgc gatcgcctcg ctgcagaatc tgccgcgcac
     1021 cgtcacggcc tcgggcacga tctcggcctg ggaagaggtg ccggtcggcg ccgaaaccgg
     1081 cgggctgacg gcgacgggcg tctatgtcga tgaaggaacc tatgtgcgcc agggccagcc
     1141 gctggtgcag atgaacgacg ccctgctacg cgcccagctg cgccagcagc aggcggcggt
     1201 ggagacggcg caggccaatg cggcccgcga tcaggccgcg ctgggccggg cccaggaact
     1261 gaaggagcgg ggcttcctca gccaggcttc gctggacacg gccctggcca accagcgcgc
     1321 ttcgagcgcc aatgtggcgg cggcgcaggc atcgctgtca gagacccgca cccgcctgtc
     1381 gcaggcgacg atcatggcgc ccgtctcggg tctggtgatc agccgcaatg tgacgcgcgg
     1441 ccagatcatc tccgccggga ccgaactgtt ccgcatcgtc cgcgacggcc ggctggaact
     1501 ggacgcccag gtgcccgaga ccgatctggc gctgatccgc tcgggccaga gcgcggtcat
     1561 ctcgtccgac cagatcggtg agacgaccgg acgcgtccgc atcgtgaccc ccgaggtcaa
     1621 cgccgagaac cgtctgggcg tggcccgcat ctcgctggtc ggcggcggct tccgccccgg
     1681 catgttcgcc cgcgcccgca tccaggtggg cgaccaaccg gcggtgacgg tgccgacggc
     1741 ctcggtgctg taccgcgaga accgcgccgg cgtcttcatg ctggggaccg acaaccgcgc
     1801 ccacttccag cccgtgaccg tgctgtcgcg cacgaccgac tcgacggccg tcagcggtct
     1861 ggccgcaggc gcgcgcgtgg tggtcgcggg ggcgggcttc ctgggcgaag gcgaccgcgt
     1921 gaccatcgcg ccggcggccc gccccgcccc tgtccgcgcc gccgctccgg cgaagaagta
     1981 aggcccggac gatgaaaaac atctcgtcct ggtcgatcaa gaatccgatc ccgatcatcc
     2041 tcatgttcgt gctgctgacg cttgcgggcg tcaacggctt catggggatg cggattaaca
     2101 acaaccccga catcgatttc ccgctgatca acgtctttgc gggccgtccc ggcgcggcgc
     2161 cggccgagat ggaggtcccg gtcacacgcc tgatcgagga ctccctggcc ggcctgccgg
     2221 gcgtgcgtca catcacgtcc caggtcacgg acggcgtctc cagcacccag atcgagttcg
     2281 agctgggcac cgacacggaa cgcgccacca acgacgtccg caacgccatg agcggcctgc
     2341 gcgccgacct gccgcaggac atgcaggaac ccagcgtcga acgtatcgac atcaccggtg
     2401 acgccctcat cacctgggtg gtccggtcct cgaccatgac gccggaagag atcagctggt
     2461 tcatcgacaa cgaggtcagc cgcgctttgc tgtccatcag cggcgtcggc gaggtcaatc
     2521 gctcgggcgg cgtggaccgc gaaatccgcg tcgagctgga tcccgaccgc ctcgcctcgt
     2581 tcggcctgac ggcggcgcag gtcagtcagg cgctgacctc cgtcaacaac gatctgcccg
     2641 gcggccgggt gaccatttcg ggctccgagc ggtcgatccg caccctcggc gccgcaggct
     2701 cgatcgaaca gctgcgcgag accctgatcc cgctggccgg cggccggtcg gcccgcctgg
     2761 gcgatctggg cacggtcacc gaccactgga gcgaaccgcg ccgtctggcc cgttacaacg
     2821 gccaggaagc ggtcaccttc aacttcctgc gctcgcgcag cgcgtccgag gtcaaggtcg
     2881 ccgagaaggt ccgcgaacgc gtcgccgaaa tcgatgaggc ccatcccgaa ctgaccatcg
     2941 aaaaggtcac ctccagcgtc gaatatatcg aggagagcta catcgcctcg atcgaggccc
     3001 tgctgctggg cgccggcctt gcggtcatcg tggtgttcat cttcctcagg gactggcgcg
     3061 ccaccttcat cacggcgacg gccatgccgc tgtcgctgat ccccgccttc gccatcctgg
     3121 ggccgctgga ccagtcgctg aacgtggtca ccctgctggc cctgtctttg acggtcggca
     3181 tcctggtcga cgacgccatc gtggagatcg agaacatcgt ccggcacatg cgggacggga
     3241 agtcgcccta tcacgcctca atggaggcgg cggacgagat cggtctggcc gtcgtggcca
     3301 ccacggcgac cattctggcc gtgttcgcgc cggtgggctt catgccgggc atcatcgggc
     3361 agttcttcaa agccttcgcc ctcgccgcct gcgtctccgt ggccttctct ctggtcgtgg
     3421 cgcggacgct gacgccactg atggccgcct atctgctgaa gcaccacaag cacgaggacg
     3481 ccgatccctt ctggatgggg acctatctga agtccctgcg ctggtccttg gccaaccgct
     3541 ggaaggtctt cgccctcggc acggtgctgt tcgtcggctg cggcttcctg gccaccaagg
     3601 tgccgttcga attctcgccc tccggcgacc agtcgcgcgc ggccttctcg atcgagctgc
     3661 cgccgggcgc gaccctgcgt cagaccgacg ccatcgtgca gcaggtcacc cgcgacctgc
     3721 ggcaacggcc agaagtcacc tcggtctacg cctcggtcgg cggtcaggac gtcaaccagg
     3781 ccaatgtcta tgccgacatg gtcggcaagg gcgaacgggc gctgagccag caggagttcg
     3841 cgcgcgagat ggtcgacggg tggaaggcca ttccgggtgc ccggatcggc gccggcacgg
     3901 gtaatcaggg cggcggacca tcggacggga ccagctatac cttcgccatc ctgagcgaca
     3961 atccggcggc cttgcaggcc gcggcccgcc agatcgaggc cgagatgcgc actgttccgg
     4021 ggctggccaa tgtggtcaac accgcctcga tcgcgcggcc ggaaatcctc gtcacgccgc
     4081 gcccggacca ggccgccctg atgggcgtct cgaccggcga catctcccag accatccgcg
     4141 tcgccaccat cggcgacgtt gcccagaacc tgcccaaata caatctgggt gaccgtcagg
     4201 tgccgatccg tctgcagctg accgaggcgg cgcgcgagga cctgtcggtg ctcgagacct
     4261 tgcgggtccc gaccgacgac ggcggcgccg tgccgctgag cgcggtggcc gacatccagt
     4321 tcggatcggg cccttcgcag gtgctgcgtc aggaccgttc gcggatcgcc tcgatcaccg
     4381 ccgaactgga tggcatccgc tcgggcgagg ccgacgccgc ggtcaagaag ctgcccacgg
     4441 tgcagaacct gcccgctggc gtgcgtcagg ttccggccgg ggacgccgag ttcatccagg
     4501 agatgatcac cggcttcgcc gtcgccttca tgaccggcat tctgctgatg tatgcggtgc
     4561 tggtgctgct gttccgcagc ttcgc
//
LOCUS       DASVVN010000205         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_32770, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000205 DASVVN010000000
VERSION     DASVVN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32770"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VF686_03170"
     CDS             complement(<1..776)
                     /locus_tag="VF686_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8660843.1"
                     /translation="MSASSIPARAGWRFWIDRGGTFTDIVARAPDGRLETRKLLSDNP
                     EQYADAAVEGVRRILNAAPGPLPEGRVDAIRMGTTVATNALLERKGEPTLLAITRGFG
                     DALRIGWQSRPDIFARNIVLNEQLYDRVVEIGERVRADGTVEQALDEDRARADLTTAH
                     SAGLRAIAIVLVHGWRFTCHETRLADIAREVGFEQVSVSHEVGALIKLIGRGDTTVAD
                     AYLSPILRAYVDRVGADLGEATPLLFMQSSGGLTAASAFRG"
     gene            complement(836..1645)
                     /locus_tag="VF686_03175"
     CDS             complement(836..1645)
                     /locus_tag="VF686_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEX8660844.1"
                     /translation="MSHAFDKTSSSPIARAFVDARRAGRVLSPYPGTAPTTLAEAYEI
                     QDEAIAAWPDRIAGWKLGRIKEPLDQQYGAGRLAGPIFSRNVWQAGAEPTRFPVIDGG
                     FAAVEAEYIIQLGDDAPDRGDWTADSVASLVGQVFTGVEIAGSPFPGINESGPAVTAS
                     DFGNNAGLILGDEISGWRDRLADLTCTVAIEGQIIGRGGSVDIPGGPLDSLAFLLNLL
                     HARGRRLEAGQLISTGAATGVHQIFIGQSSVADFGPDGRIACEAVAATPTA"
     gene            1817..2860
                     /locus_tag="VF686_03180"
     CDS             1817..2860
                     /locus_tag="VF686_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8660845.1"
                     /translation="MNETRRTGGKPATINDIARLAEVSKKTVSRVINNSPFVKEDTRK
                     RVEAVIAEHGYSPDPQARGLAFRRSFLVGMIYDNPSPNYVVNMQQGVLDAVRGSGLEL
                     LVHPCNRGSDTFLHEVQAFIVRQKLFGVVLPPSVSEDDRVVAILREADCPYVRIASVS
                     LDEPARMVVTHDSRGGARAARHLAELGHRRIAFISGPDTFRSSHERGRGFAEGLAEHG
                     VTLDPAYIRRAAYTFESGVEAATELLALPQRPTAIFAGNDEMAIGVMKAARDAGLRVP
                     EDLSIVGFDDLPMASRVWPNLTTVRLPIRDMGRMAAEKLTARSRGLDPATLVQPEVDP
                     SLVVRDSTAPPGR"
     gene            complement(2864..3184)
                     /locus_tag="VF686_03185"
     CDS             complement(2864..3184)
                     /locus_tag="VF686_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660846.1"
                     /translation="MTSITGAGWLYQVHHLVPVNFDQWSMLKAVRDVGLICVALIWLF
                     RPSRALIAAAMSAVFVAPAFISANPPDLPYVMISLACVGVALITTQVRRGIPSPFNKG
                     AAPT"
     gene            complement(3331..>3692)
                     /locus_tag="VF686_03190"
     CDS             complement(3331..>3692)
                     /locus_tag="VF686_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8660847.1"
                     /translation="VSPALIRYAALFNQRDWPALRALLADDVKLVQSSRAPREGSSEV
                     GLFFTVYAGIEGVRLAPAWLEGREVAAVFESADALHPAYVMWLDWRDGRISFIRDYRY
                     SRYVLADAELTLVSSPG"
BASE COUNT          603 a         1297 c         1241 g          551 t
ORIGIN      
        1 ccccggaagg cgcttgccgc cgtcagcccg ccgctggact gcatgaacag cagcggcgtc
       61 gcctcgccca gatcggcgcc gacccggtcc acataggcgc gcaggatcgg ggacagatag
      121 gcgtcggcca ccgtggtgtc gccgcgcccg atcagcttga tcagcgctcc gacctcgtga
      181 ctgaccgaca cctgctcgaa gccgacctcg cgggcgatgt cagccagccg cgtctcatgg
      241 cacgtgaacc gccagccgtg caccagcacg atggcgatgg cgcgcaggcc cgccgagtgg
      301 gccgtagtca gatcggcgcg ggcccggtct tcgtcgaggg cctgttcgac ggttccatcc
      361 gcgcggacgc gctcgccgat ttcgacgacg cggtcataga gctgttcgtt caggacgatg
      421 ttgcgggcga agatgtcagg ccggctttgc cagccgatgc gcagcgcgtc gccgaagccg
      481 cgcgtgatgg ccagcagggt cggctcgccc ttgcgttcca gcagggcgtt ggtcgccact
      541 gtggtgccca tgcggatggc gtcgacccgg ccttcgggca gaggtcccgg cgcggcgttc
      601 aggatgcggc gcaccccctc gaccgccgcg tcggcgtact gttccggatt gtcggacaga
      661 agtttgcgcg tctccagccg cccgtccggc gcccgggcga caatgtcggt gaaggtgccg
      721 ccgcgatcga tccagaagcg ccagccggcc cgagcaggga tcgatgacgc gctcatgggt
      781 tcgctccggc ggcaggagcg atgcagatag ccggtggcgg cgccgtccgt ctacgtcagg
      841 cggtcggagt cgccgccacg gcctcgcagg cgatccgccc gtcgggtccg aagtcggcga
      901 ccgaggactg gccgatgaag atctggtgca ccccggtcgc cgcgcccgtc gagatcaact
      961 gccccgcctc cagccgccgg ccgcgcgcat gcagcaggtt cagcaggaag gccagcgagt
     1021 ccagcggccc gcccggaatg tccacggagc cgccccggcc gatgatctgg ccttcgatcg
     1081 cgaccgtgca ggtcaggtcc gccagccgat cccgccagcc ggagatctcg tcccccagaa
     1141 tcagccccgc gttgttgccg aagtcggacg ccgtcaccgc cggtcccgat tcattgatgc
     1201 ccgggaacgg gctgccggcg atctccaccc cggtgaagac ctgtccgacc agcgacgcca
     1261 cggagtcggc ggtccagtcg ccccggtcgg gcgcatcgtc gcccagctgg atgatgtact
     1321 ccgcctcgac cgccgcgaag ccgccgtcga tcaccgggaa gcgcgtcggt tccgccccgg
     1381 cctgccagac gttgcgcgag aagatagggc ccgccagccg gcctgcgccg tattgctggt
     1441 ccagcggttc cttgattcgc cccagcttcc agcccgcgat ccggtccggc caggcggcga
     1501 tcgcctcgtc ctggatctca taggcctcgg ccagggtggt gggcgccgtt ccgggatagg
     1561 gcgacaggac ccgcccggcg cggcgcgcat cgacgaaggc gcgcgcgatc ggcgacgaac
     1621 tggttttatc gaatgcgtgg ctcacgccgc ctccccgttt ggcgcggaca ttgcgcagga
     1681 aggaaaccgg tgtcaatttg aatccggcgg cgcttgttct gcgacgccgg cgaccggtgg
     1741 tgcgccgcgg gacgagaccg ctgacagacg ggacgccaga ggctaaggac aaggttcggc
     1801 cagagagaac cccgacgtga acgaaacccg acggacaggc ggaaagcccg ccaccatcaa
     1861 cgacatcgcc cggctggccg aggtgtcgaa gaagaccgtc tcgcgggtca tcaacaactc
     1921 gcccttcgtc aaggaggaca cccgcaaacg agtcgaggcg gtgatcgccg aacacggcta
     1981 cagcccggat ccccaagccc ggggtctggc cttccggcgc tcgttcctgg tcgggatgat
     2041 ctacgacaac cccagcccga actatgtcgt caacatgcag cagggcgtgc tggacgcggt
     2101 gcgggggtcg ggcctcgaac tgctggtcca tccgtgcaat cgcggctcgg acaccttcct
     2161 gcacgaggtc caggccttca tcgtccggca gaagctgttc ggcgtcgtcc tgcccccctc
     2221 ggtgtccgag gacgaccgcg tcgtcgctat cctgcgcgag gccgactgcc cctatgtccg
     2281 catcgcctcg gtctctctcg acgagccggc ccgcatggtc gtgacccacg acagccgcgg
     2341 cggagcccgc gccgcgcggc atctggcgga actggggcat cgccgcatcg ccttcatctc
     2401 gggccctgac accttccgct cgtcgcacga acggggcagg ggattcgccg aaggtctggc
     2461 cgagcacggc gtgaccctcg atcccgccta catccgacgc gccgcctata cgttcgagtc
     2521 gggcgtcgag gcggcgaccg aactgctggc cctgccgcag cggcccacgg ccatcttcgc
     2581 cggcaacgac gaaatggcca tcggggtcat gaaggccgcg cgcgacgccg gtctgcgtgt
     2641 gcccgaggat ctgtccatcg tcggtttcga cgacctgccc atggcctcgc gcgtctggcc
     2701 caacctgact acggtgcgtc tgccgatccg tgacatgggc cggatggccg ccgagaagct
     2761 gacggcccgc agccgcggcc tcgaccccgc caccctcgtc cagcccgagg tcgatccgtc
     2821 gctggtcgtg cgggattcga cggcgccgcc ggggcggtag gcgtcaggtc ggcgccgcac
     2881 ccttgttgaa cggcgacggg attccgcgcc tcacctgggt ggtgatcagg gcgacgccca
     2941 cgcatgccag cgagatcatg acatagggaa gatccggcgg gttcgcgctg atgaaggcgg
     3001 gcgcgacgaa caccgccgac atcgccgctg cgatcagggc gcgcgagggg cggaacagcc
     3061 agatcaaggc tacgcatatc aggccgacgt cgcggaccgc cttgagcatc gaccattggt
     3121 cgaagttcac gggcacgaga tgatggacct gatacagcca gccggctccg gtgatggagg
     3181 tcactgacaa caggccgaaa gccgccagcc tgaacattcc gaacctcgca tgcgtcgcag
     3241 agtggtgaag ggtggcgcgg ccgggtggac ccgtgcgcct cgcgccgcgg catcagttgg
     3301 tggatggcgg cgcgttgcac aatgggaaca tcaccccggg gacgaaacca atgtcagttc
     3361 cgcgtcggcc aggacgtaac ggctgtatct gtaatcccgg atgaaactga tccgtccgtc
     3421 gcgccaatca agccacatca catatgcagg gtgcagggcg tccgcgcttt cgaagacggc
     3481 cgccacctca cgcccctcca gccaggccgg cgccagtcgg acgccctcga tcccggcgta
     3541 gaccgtgaag aacaggccca cttcggatga gccctcccgg ggcgcccgcg acgactgcac
     3601 cagtttcaca tcatcggcca gcagtgctcg caacgccggc caatctcgct gattgaacag
     3661 cgcggcgtat cggatcaacg ccggcgagac gg
//
LOCUS       DASVVN010000206         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_85443, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000206 DASVVN010000000
VERSION     DASVVN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85443"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF686_03195"
     CDS             complement(<1..142)
                     /locus_tag="VF686_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX8660848.1"
                     /translation="MAPRIARLDSRALIRVTGPDTRPFLHNLLTQDVETLAAGQLRFG
                     ALL"
     gene            227..1186
                     /locus_tag="VF686_03200"
     CDS             227..1186
                     /locus_tag="VF686_03200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX8660849.1"
                     /translation="MASRFPILYIAEADASDAILSSGVLAYLVEAMPQASFTIVGSPK
                     SAPLFADTPRLDRLIVLERDSRLDWLGLWNKVRETRWGLVVDMRGTTLSSKLKRQKRA
                     VRGAWEAGVHAVEQAARVLQLEEIPAPKLFVSGTTQARADALIPAEDVPILALGPGAD
                     WMGKIWPSERYAKIAVALVGDGGPLEGGRVIVVGDDNARDAAHTVRLSLPRNRVTELQ
                     GRLSRLETVAALGRAALYVGADTIWTDLAVASGAPVVAAFGPSDEVEHGPWGGVAVRG
                     PRSVEEYRKIDPNLNQAIQHMNDLPADRVLKAATKLLAEQNNA"
     gene            1290..>1593
                     /locus_tag="VF686_03205"
     CDS             1290..>1593
                     /locus_tag="VF686_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660850.1"
                     /translation="MGPRDKPEDDGVEMNYDLIVRGGEVANHAGRGMADVGVTDGRIV
                     FVGDLSQASAGEVVDATGLTVLPGVIDTQVHFREPGLEWKEDLETGSRGAVLGGVTA"
BASE COUNT          261 a          512 c          586 g          234 t
ORIGIN      
        1 acagcagggc gccgaagcgc agctgtcccg ccgccagggt ctcgacgtcc tgcgtcagca
       61 gattatgcag gaagggccgc gtgtcggggc cggtcacgcg gatcagggcg cggctgtcga
      121 gtcgggcgat gcgaggggcc atgacgccta gataggggcc cgcgcgcctg tggaccacac
      181 ctgtgacacg cctgatgcgg cgagagaatc cgtatacggt cgatccatgg cgagccggtt
      241 tcccattctg tacatcgccg aggccgacgc gagcgacgcg atcctgtcgt cgggggtgct
      301 cgcatatctg gtcgaggcca tgccgcaggc cagcttcacc attgtcggtt cgccgaagag
      361 cgcgcctctg ttcgccgaca cgccgcgcct ggatcgtctg atcgtactgg agcgcgacag
      421 ccggctggac tggctgggcc tctggaacaa ggtgcgggag acccggtggg ggctggtggt
      481 cgacatgcgg ggcaccaccc tgtccagcaa gctgaagcgc cagaagcggg ccgtgcgcgg
      541 ggcttgggag gcgggcgtcc atgccgtcga acaggccgcg cgggttctgc agctcgagga
      601 aatcccggct ccgaaactgt ttgtgtcggg gacgacgcag gccagggccg acgcactgat
      661 ccccgccgag gacgtcccga tcctcgccct cggtcccggc gcggactgga tgggcaagat
      721 ctggccgtcc gaacgttacg ccaagatcgc ggtcgcgctg gtcggcgacg gcgggccgtt
      781 ggagggtgga cgggtcatcg tcgtcggcga cgacaacgcc cgtgacgcgg cccataccgt
      841 acggctgtcg ctgccgcgga accgggtcac cgaactgcag ggacggttga gccggctcga
      901 gaccgtcgca gccctgggcc gggcggcgct ttacgtcggc gcggacacga tctggaccga
      961 tcttgcggtg gccagcggcg cgcccgtggt cgcggctttc gggccgtcgg acgaggtcga
     1021 gcacggcccg tggggcgggg tcgccgtgcg cgggccgcgc tcggtcgagg aataccgcaa
     1081 gatcgacccc aatctgaacc aggccatcca gcacatgaac gacctgccgg ccgaccgggt
     1141 gctgaaggcg gcgacgaagc tgctggccga acagaacaac gcctgaatcc gtcatcctcg
     1201 ggcttgtccc gaggacccat ccgtccgcat aagcgagcgg atgatttgat cgccggtctg
     1261 gaagcacgaa cgaccatgcg accgcaaaaa tgggtcctcg ggacaagccc gaggatgacg
     1321 gtgttgaaat gaactacgac ctgatcgtcc gcggcggcga agtggccaac cacgccggac
     1381 gcggcatggc cgacgtcggc gtgaccgacg gccggatcgt cttcgtcggc gacctgtcgc
     1441 aagcctcggc gggcgaggtg gtggacgcga ccggcctgac cgtcctgccc ggcgtcatcg
     1501 acacccaggt ccatttccgc gagccgggcc tggagtggaa ggaggacctg gagacagggt
     1561 cgcgcggggc ggtgctcggc ggcgtcaccg ccg
//
LOCUS       DASVVN010000207         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_480177,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000207 DASVVN010000000
VERSION     DASVVN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480177"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1149
                     /locus_tag="VF686_03210"
     CDS             145..1149
                     /locus_tag="VF686_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8660851.1"
                     /translation="MPSSRRLLAVAVAVAASAGLIGCGQASGRSMAATTSDAASQRLT
                     TLTSLDQRVARVAQRLSEDNVDLCPAVRQSAGWALHSASQYSNELRPLVQSRFGLEGD
                     LPGVLSAPPGSAAAEAGLREGDLILSVNGEALNPGPARTRPAFEGLAANIAMIDRALA
                     GGATRLGIRRNDEPLDVTVQPHRACGYEVQLNPSDELNARADGRRLFISTALAGFTQS
                     DDELALILGHELAHHVLRHRTWSDVGGAGREANDAAAVNGAGDKETQADRVGLFLTAR
                     AGYDTRIAPAFWRRFGAFNWRVRYPQLRHASAEARARALEQVQVEIDAKRARGGPLLP
                     "
     gene            complement(1153..>1567)
                     /locus_tag="VF686_03215"
     CDS             complement(1153..>1567)
                     /locus_tag="VF686_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660852.1"
                     /translation="AADRAPGHDIVVLLQPTSPLRTADDIEATLAVMVGTGAPGAVSV
                     SEAPCHPYLIFQRDPAGRLSPFVEKPAEVGWRRQDLPPAYRVNGAVYAADLAWLRAER
                     TLCKASETAAYEMPVERSIDIDTLEDLQAAERAPG"
BASE COUNT          229 a          565 c          547 g          226 t
ORIGIN      
        1 gccaaagatg gttgctggcc gttaaggaac cgaaaaggcg acgcttgccg accgctaatg
       61 cggcggaaaa gcgggttgcc tcatcgggct ccatcctgtc tgtgggggaa gtcggttgtc
      121 catgccgacg atcgaaaccg agccatgccg tccagccgcc gcctgctagc cgtcgccgtc
      181 gccgtcgccg cttccgcggg cctgatcggc tgcggacagg cgtcgggcag atccatggcg
      241 gccacgactt cggacgccgc gtcgcagcgg ctgacgacgc tcacatcgct ggaccaacgc
      301 gtcgcgcggg tggcgcagcg gctgtccgag gacaacgtcg acctgtgtcc tgcagtccgg
      361 caatcggccg gatgggccct gcattccgcc agccagtaca gcaacgaact gcggcctctg
      421 gtccagtcgc ggttcggtct ggagggcgac ctgccgggcg tcctgtccgc gcctccgggt
      481 tcggccgccg cggaagcggg gctgcgcgag ggcgatctga tcctgtcggt caacggcgag
      541 gcactgaacc ccggcccggc ccgcacaagg cccgctttcg agggtctggc cgccaacatc
      601 gcaatgatcg accgcgccct ggccggcggc gcgacccgcc tcggcatccg ccggaacgac
      661 gaaccgctgg acgtgaccgt ccagccgcac cgggcctgcg gttatgaggt gcagctgaac
      721 ccctcggacg aactgaatgc gcgcgccgac gggcggcggc tgttcatcag cacggccctg
      781 gccggcttca cccagtccga cgacgagctg gccctgatcc tcggccacga gttggcccac
      841 cacgtcctgc ggcaccgcac ctggagtgac gtcggcggcg cgggccgcga ggccaatgac
      901 gcggcggccg tcaacggcgc tggcgacaag gagacgcaag ccgatcgggt cggcctgttc
      961 ctgaccgcga gggccggata cgacacccgg atcgcccccg ccttctggcg ccggttcggc
     1021 gctttcaact ggcgcgtccg ttatccgcag ctgcgccatg cctcggccga ggcccgcgcc
     1081 cgcgcgctgg aacaggttca ggtcgagatc gacgccaaac gggcgagggg cgggccgctg
     1141 ctgccctgac ggtcagccgg gcgcgcgttc ggccgcttgc aggtcttcaa gggtgtcgat
     1201 gtcgatggac cgctcgaccg gcatttcgta cgccgccgtc tcgctcgcct tgcacagcgt
     1261 ccgttcggcc ctcagccacg ccagatccgc cgcatagacg gccccgttga cgcgatacgc
     1321 gggcggcagg tcctgacgtc gccagcccac ctccgccggc ttctcgacga agggactcag
     1381 ccggcccgcc gggtcacgct ggaagatcag atacggatgg cagggcgcct cgctgaccga
     1441 caccgcgccg ggcgcgcccg tcccgaccat gacggccagc gtggcctcga tgtcgtcggc
     1501 cgtccgcagg ggcgaggtcg gctgcagcag cacgacgatg tcatggccgg gcgcgcggtc
     1561 ggcggcg
//
LOCUS       DASVVN010000208         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_243604,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000208 DASVVN010000000
VERSION     DASVVN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243604"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VF686_03220"
     CDS             complement(<1..802)
                     /locus_tag="VF686_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660853.1"
                     /translation="MSSIIRATPAQMTRRVVVLAAAIFAIVVGQGQMALGLGQTAAEF
                     SADSDATLKVAGYAFAIWGVIYLGLLIYAVRQVLPQTGESLLIHRLGWPSAAAMTGIG
                     LWIVAAAMDAEMATIVLIFGSLVVLLVPMLLNAGAIRVLPVRDRDRWLVVWPLAMLAG
                     WLTVAAPLNLITVATGNGDLPAALSPTVWAMIAIAVVAVVALAVTQRIRTMAYAMPVA
                     WGLLGAFVAEQPRNSSLAWFALAAAVAVLSGTIVLSLGLRRGVERREAV"
     gene            916..1152
                     /locus_tag="VF686_03225"
     CDS             916..1152
                     /locus_tag="VF686_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660854.1"
                     /translation="MADPDRPTPESPVFETDSVQADRAREQGLGLGERELAAQRDPGG
                     VRTPDESDPDALDQSVGVQGSDDDEDSRTANPAV"
     gene            1198..>1535
                     /locus_tag="VF686_03230"
     CDS             1198..>1535
                     /locus_tag="VF686_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8660855.1"
                     /translation="MAENTDRRTLESGLADADRALAANPRDLRALIAKGDALVGLGDA
                     RAGASFYGSALRGAAEVANLPPDLVAELRRAQAAIEDQSTRFQSYLTDSLARSGFDAA
                     TSSDRFTQSL"
BASE COUNT          272 a          544 c          509 g          210 t
ORIGIN      
        1 tgaccgcttc gcgccgttcg acgccgcggc gcaggcccag gctcagcacg atcgtcccgg
       61 acaggaccgc gacggccgcg gccagcgcga accaggcgag gctggagttg cgcggttgtt
      121 cggcgacgaa ggcgcccagc aggccccaag cgacgggcat ggcgtaggcc atggtccgta
      181 tccgctgggt cacagcgagc gcgaccacgg cgactaccgc gatggcgatc atcgcccaca
      241 ccgttggcga caaggccgcg ggcaggtcgc cgtttccggt cgccacggtg atcaggttca
      301 atggcgcggc caccgtcagc catccggcca gcatcgccag cggccagacc accagccagc
      361 gatcgcggtc ccggacgggc aggacgcgga tggcgcccgc gtttagcaac atcgggacca
      421 gcagcacgac gagggagccg aagatcagca cgatggtcgc catctccgca tccatcgccg
      481 ctgcgacgat ccagaggccg attcccgtca tcgcggccgc cgacggccag cccaggcggt
      541 ggatcagcag gctctcgcct gtctgcggca gcacctgccg caccgcatag atcaggaggc
      601 cgagatagat gaccccccag atggcgaagg cgtagccggc gaccttcaac gtcgcatcgc
      661 tgtccgcgga gaactcggcg gcagtctgac caagacccag ggccatctga ccttggccga
      721 ccacgatggc gaagatcgcg gcagccagca cgacgacccg acgggtcatc tgggcgggcg
      781 tggcgcggat gatggaggac atcggcatct cccaagggct ctggacacaa ctacgttcgg
      841 ccgcggctaa aggttcaccc ccggaaccgt ggcgtctcga cgcgggttga atcggcctca
      901 gcaatggaga cgaccatggc cgatcccgac cgtcccaccc ccgaatcacc cgtcttcgaa
      961 accgacagcg tgcaggccga ccgcgcccgc gagcagggtc tcggcctcgg cgagcgcgaa
     1021 ctggccgccc aacgcgatcc cggcggcgtc cgcacgcccg acgagagtga cccggatgcg
     1081 cttgatcagt ctgtgggcgt acagggctcc gacgatgacg aggattcccg cacggcgaat
     1141 cccgccgtct gacggtttcg ccttgacgga cgtcgctcgc cgacctccga tcagcgaatg
     1201 gcggaaaaca ctgatcgtcg aacgctcgaa tccggattgg cggatgccga ccgggcgctc
     1261 gccgccaacc cgcgcgatct tcgcgccctg atcgccaagg gcgacgccct tgtcggtctg
     1321 ggggatgcgc gcgccggcgc cagcttctac ggttcggccc tgcgcggcgc cgccgaggtc
     1381 gcgaacctgc cgccggacct cgtagcggag ctccggcggg cgcaggcagc aatcgaggac
     1441 cagagcacgc gctttcagtc ctatctgacc gatagtctgg cgaggtccgg ttttgacgcc
     1501 gctacctcca gcgaccggtt cactcagtca ctgga
//
LOCUS       DASVVN010000209         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_218199,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000209 DASVVN010000000
VERSION     DASVVN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218199"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1048)
                     /locus_tag="VF686_03235"
     CDS             complement(134..1048)
                     /locus_tag="VF686_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660856.1"
                     /translation="MRRFFVWSALAAVIGLALAIGGFWAYHQFYARFQPVTVERNQKP
                     IQDLLEQSSWISAGGGGEPLYIIGYRDSAAMQRYEREEVPKLRAAGVEARIILFARPD
                     REGLAQSTAAERATVAELWLTRDWTLYQRWTATPVRNWTAAGIPAADGNLARGAVVEA
                     GRNFIASLSEKLGEEGLQTRYPLVIWRDREGFMKACACSDSRSWAFIRDDLDAPDRIE
                     APGIAPGDPAAPAAYPGDGSPESLPYPTLPAPADGSVPVPAPAPAQIPGTTPGTAPGT
                     RPIAPRPAAPPVPRKAPEPKQAEDTTFF"
     gene            complement(1135..1542)
                     /locus_tag="VF686_03240"
     CDS             complement(1135..1542)
                     /locus_tag="VF686_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660857.1"
                     /translation="MVWPIIREKRRGWRRRPPASRPALGGDAPRAWCLLAELDGRDIG
                     YLSWTRPYGIWRGGDYLNLDDLYVNEAARGRGVGEALMRRFAGIAAVQSLPARWEVKS
                     ENDAARRFYTRLGADQSDKTIVRWSVGAMLAVG"
     gene            complement(1612..>2309)
                     /locus_tag="VF686_03245"
     CDS             complement(1612..>2309)
                     /locus_tag="VF686_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8660858.1"
                     /translation="TGAAFGSAGERCMALPVAVPVGDETAERLRAALGPAIEALRVGV
                     STDAGAHYGPVVTAQHKARVEGWIDQGVKDGAELVVDGRGFTLQGHEKGYFIGPSLFD
                     HVTPGMESYKEEIFGPVLQIVRAASFEEALALPSKHQYGNGVAIFTQNGRAARDFAAR
                     VNVGMVGINVPIPVPVAYHTFGGWKRSAFGDTNQHGMEGVKFWTKVKTITARWPDSDL
                     EHADSSFVIPTMG"
BASE COUNT          357 a          783 c          813 g          356 t
ORIGIN      
        1 gtgcatggct cgacgcgttt ttaacccggc gtgctcgcta ccaagagtgt aaattaccct
       61 taatggttca acttaaggtg attggctagg tgcgggcccg ccgacacgtc ggaaggcgcc
      121 ggggaccgca ggttcagaag aatgtcgtgt cctccgcctg cttcggttcc ggggccttgc
      181 gcgggacggg cggcgccgca gggcgtggcg cgatcggacg ggttccgggc gcggttcccg
      241 gcgtggttcc tggtatctgg gccggagccg gagccggaac cggaaccgag ccgtcggccg
      301 gcgccggcag ggtcggatag ggcaggctct cggggctgcc gtcgccgggg taggcggcgg
      361 gggccgccgg atcgccgggc gcgattcccg gcgcttcgat ccggtccggt gcgtccaggt
      421 cgtcgcggat gaaggcccag gaccgcgagt ccgaacaggc gcaggccttc atgaagccct
      481 cgcggtcgcg ccagatgacc agcgggtagc gcgtctgcag cccttcctcg ccgagctttt
      541 cggacaggct ggcgatgaag ttccggcccg cctcgaccac ggcgccgcgc gccagattgc
      601 cgtcggcggc gggaatgccc gcggcggtcc agttgcgcac cggggtggcg gtccagcgtt
      661 ggtacagggt ccagtcccgc gtcagccaca gctcggccac cgtcgcgcgc tcggcggccg
      721 tggattgggc gaggccctcg cggtccggcc gggcgaacag gatgatccgg gcctcgacgc
      781 ccgcggcgcg cagtttgggc acttcttccc gctcgtagcg ttgcatggcc gccgagtcgc
      841 gatagccgat gatgtagagc ggctcgccgc cgccgccggc cgagatccat gaagactgct
      901 cgagcagatc ctggatcggc ttctgattgc gctccaccgt cacgggctgg aagcgggcgt
      961 agaactggtg gtaggcccag aaaccgccga tggcgagggc caggccgatc acggccgcca
     1021 gggcggacca gacgaaaaag cgacgcatac gggggagcgc tccagcttca ctgttcagac
     1081 agcataacgc ctatcaccga aatgcgatgc cgcgaacgcc ggagaggcga acgatcaacc
     1141 gacggccaac atcgccccga cagaccaacg gacgattgtc ttgtcggact ggtcagcgcc
     1201 gaggcgggtg tagaaccgac gagccgcgtc gttctcggac ttaacctccc agcgggcggg
     1261 caagctctgg accgcggcga tcccggcgaa acgccgcatc agcgcctcgc cgaccccgcg
     1321 gcctcgcgcc gcctcgttca catacaggtc gtcgaggttc agatagtcgc cgccgcgcca
     1381 gattccgtac ggccgtgtcc aagacagata gccgatgtcc cggccgtcca actccgccag
     1441 caggcaccac gcccggggcg cgtctccgcc gagggcgggc cttgaggctg gcggacgtcg
     1501 ccgccagccc cgacgtttct ccctgatgat cggccagacc aagcatcagc gcatgcacgc
     1561 ggacggcgtc gtcgggttcg gcgcgccgaa tggccaccgt cgccattccg tttaacccat
     1621 ggtcgggatg acgaagctgg aatcggcgtg ttccagatcg ctgtcgggcc agcgggcggt
     1681 gatggtcttg accttggtcc agaacttcac cccctccatg ccgtgctggt tggtgtcgcc
     1741 gaaggccgag cgcttccagc cgccgaaggt gtggtaggcg accggcaccg ggatcgggac
     1801 attgatcccg accatgccga cattgacccg ggcggcgaag tcgcgggccg cgcggccgtt
     1861 ctgggtgaag atggcgacgc cgttgccgta ctggtgcttc gacggcagcg ccaaggcttc
     1921 ctcgaagctg gcggcgcgga cgatctgcag caccgggccg aagatctcct ccttgtagga
     1981 ttccatcccg ggggtgacgt ggtcgaacag cgacgggccg atgaaatagc ccttctcgtg
     2041 cccctgcagg gtgaagccgc ggccgtcgac gaccagttcg gcgccgtcct tgacgccctg
     2101 gtcgatccaa ccttcgacgc gggccttgtg ctgggccgtg acgaccgggc cgtagtgggc
     2161 gccggcgtcc gtcgagacgc cgaccctgag cgcctcgatt gccggcccca gggccgcgcg
     2221 gagccgctcc gcggtctcgt cgccgaccgg caccgccacc ggcaaggcca tgcaccgctc
     2281 gccagccgag ccgaacgccg cgccagtga
//
LOCUS       DASVVN010000210         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_428263,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000210 DASVVN010000000
VERSION     DASVVN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428263"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VF686_03250"
     CDS             <1..301
                     /locus_tag="VF686_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phthalate transporter"
                     /protein_id="HEX8660859.1"
                     /translation="RLGVVWRFMFYGGLLAAGLQAMLALIVSVVGWFEAGGIAQAAGA
                     TETTLLIYGVGGLPIAVLLGVSYALWAGLCAAFIAFMPQPEVRDRLGVMRDRAPD"
     gene            383..1651
                     /locus_tag="VF686_03255"
     CDS             383..1651
                     /locus_tag="VF686_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX8660860.1"
                     /translation="MAAIDIESAPRAAPALGPVQPKDRIYNLDVLRGWAILGILAVNA
                     IAFAWPFQLTMSEAPPPGVLTQADHIGMWVTDVFFSDKMRTLFTMLFGVSVFLVGGER
                     FDDARGKLLRRRLLWLGLIGLIHGAVFWYGDILLHYAYCGLLVMLMRSWSARRLLWVG
                     GSITLLWGLVATAGYWGMANMPAEFAAQMESGMPAADPAAIQATIDLYRADWPSALLQ
                     NLQTWATFQLMASPFLIPVTVPLMLLGLGLFKAGWFSGRAPLWTYLLVMAVGGANLAA
                     FAWFGWQEAMAPEGTDPTNGLAAAAGGMAFLITLFYASVLILINRFGLRVLTAVFAPV
                     GRMAFTNYLTQTLIMVTLYYSPWGPVWFGSHSPSEMWMVVIAVWIAQLIWSPLWLSVF
                     SMGPLEWVWRCLTYGRMVPIRKPAAASVAL"
     gene            1691..>2455
                     /locus_tag="VF686_03260"
     CDS             1691..>2455
                     /locus_tag="VF686_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HEX8660861.1"
                     /translation="MSTDTPTRPEARNPRGFADRRGADLTAERRLVARVSEVYERWGF
                     EPLETGAFEYADALGKFLPDADRPNEGVFALQDDDEQWMALRYDLTAPLARFAAQSWE
                     TLPKPFRRYAFGPVWRNEKPGPGRFREFVQCDADTVGSDRPEADAEIIAMACEGLQAA
                     GLSADQAVVRVSNRKLFDGLFAAGGIEDAGQKLTALRAIDKFDRLGWEGVAALLGEGR
                     LDESGDYTKGAKLPSGVIATIEGFLASVGTEGLTRAQ"
BASE COUNT          323 a          850 c          845 g          437 t
ORIGIN      
        1 gcggctgggc gtggtctggc gtttcatgtt ctacggcggc ctgctggcgg cggggcttca
       61 ggcgatgctg gccctgatcg tgtcggtggt gggctggttc gaggccggcg gcatcgccca
      121 ggcggcgggc gcgaccgaga ccacgctgct gatctacggc gtcggcggcc tgcccatcgc
      181 cgtgcttctg ggcgtcagct acgccctgtg ggccgggctg tgcgcggcct tcatcgcctt
      241 catgccccag cccgaggttc gcgatcgtct gggcgtcatg cgcgatcgcg ccccggactg
      301 acggcccgcc ggttacggcg aattcagttt ccagaaatcg acgcttccgc cactgtgacg
      361 cccgccggga cgggggaaga tcatggctgc aatcgacatt gaatccgcgc cgcgggccgc
      421 acccgcgctc gggcccgtcc agccgaagga ccgcatctac aatctggatg tgctgcgcgg
      481 ctgggcaatt cttggcatcc tggccgtcaa cgccatcgcc ttcgcctggc cgttccagct
      541 gaccatgtcc gaggcgccgc ctcccggcgt gctcacccag gccgaccaca tcggcatgtg
      601 ggtcaccgac gtcttcttct ccgacaagat gcggacgctc ttcaccatgc tgttcggcgt
      661 gtcggtcttt ctggtcggcg gcgagcggtt cgatgatgcg cgcggaaagc tgctgcgccg
      721 gcgtctgctg tggctgggcc tgatcggcct gatccacggc gccgtcttct ggtacggcga
      781 catcctgctc cactacgcct actgcggcct gctggtgatg ctgatgcgct catggagcgc
      841 gcgcaggctg ctgtgggtcg gggggtcgat caccctgctg tggggcctcg tcgccaccgc
      901 cggctactgg ggcatggcca atatgccggc cgagttcgcg gcgcagatgg aatcgggcat
      961 gccggcggcg gaccctgccg caattcaggc cacgatcgat ctctaccgcg ccgactggcc
     1021 ctccgccctg ctccagaacc tgcagacctg ggccaccttc cagctgatgg ccagcccgtt
     1081 cctgattccg gtcaccgtgc cgctgatgct gctggggctg ggcctgttca aggcaggctg
     1141 gttctcgggc cgggcgccgc tgtggaccta tctgctggtc atggcggtcg gcggcgccaa
     1201 tctggcggcc ttcgcctggt tcggctggca ggaggcgatg gcgcccgagg ggacggaccc
     1261 caccaatggc ctggccgccg ccgccggcgg catggccttc ctgatcaccc tgttctacgc
     1321 cagcgtcctg atcctgataa accggttcgg actgcgggtg ctgaccgccg tcttcgcccc
     1381 ggtcgggcgg atggccttca ccaactatct gacgcagacc ctgatcatgg tcaccctcta
     1441 ctattcgccc tggggtcccg tgtggttcgg ttcgcacagt ccctcggaga tgtggatggt
     1501 ggtgatcgcc gtctggatcg cccagctgat ctggtcgccg ctgtggctgt cggtgttcag
     1561 catggggccg ctggagtggg tgtggcgctg cctgacctac ggccggatgg tgccgatccg
     1621 caaacccgcc gcggcctcag tggccctctg agcgttgccc ggggacgggt gatggcttaa
     1681 gcggcgcggc atgagcaccg acacgccgac ccgccccgaa gcccgcaatc cgcgcgggtt
     1741 cgccgaccgc cgaggcgcgg acctgaccgc cgagcgccgc ctggtggcgc gcgtgtccga
     1801 ggtctatgag cgctggggtt tcgagccgct ggagaccggc gctttcgaat acgccgacgc
     1861 cctgggcaaa ttcctgcccg acgccgaccg tccgaacgag ggcgtcttcg ccctgcagga
     1921 cgacgacgaa cagtggatgg ccctgcgcta cgacctgacc gcgccgcttg cccggttcgc
     1981 ggcccagagc tgggagacgc tgcccaagcc tttccgccgc tacgccttcg gccccgtctg
     2041 gcgcaacgag aagcccggcc ccggccgctt ccgcgaattc gtccagtgcg acgccgacac
     2101 ggtcggctcc gaccggcccg aggctgacgc cgagatcatc gccatggcct gcgagggctt
     2161 gcaggcggcg ggtctgagcg ccgatcaggc cgtggtccgg gtctcgaacc gcaaactgtt
     2221 cgacggcctg ttcgcggcgg gcgggattga ggacgccggc cagaagctga ccgcgctgcg
     2281 cgccatcgac aaattcgacc gtctgggctg ggagggcgtc gccgccctcc tgggcgaggg
     2341 ccggctggac gagtcaggcg actacaccaa gggagcgaaa ctgccctccg gcgtgatcgc
     2401 gaccatcgaa ggcttcctcg cctcggtcgg aaccgaaggc ctcacgcggg cccag
//
LOCUS       DASVVN010000211         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_7093, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000211 DASVVN010000000
VERSION     DASVVN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7093"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF686_03265"
     CDS             complement(<1..521)
                     /locus_tag="VF686_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX8660862.1"
                     /translation="MKALILNGTLKPSPETSSTEALARVVLAELEKGGAEVEMIRLVD
                     LNLKPGVKTDQGEGDDWPAVHAKIMAADILVFATPTWLGQMSSVCLRALERMDALFGE
                     TDDSGRPVAFGKVAGVVITGNEDGAHHIVATVCQALIDMGFTIPGQSWTYWHLGPGPG
                     PDYVETEQGHEYS"
     gene            654..1001
                     /locus_tag="VF686_03270"
     CDS             654..1001
                     /locus_tag="VF686_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8660863.1"
                     /translation="MQTDPLSAKFAALADPTRRAILARLCEGEASVNDLAEPFDMSLP
                     AVSKHLKVLEKAGLISRGREAQWRPARLEPMGMKGIAEWLEHYRRYWDASFDRLDAYL
                     KKIQKGDPNGPRN"
     gene            985..1491
                     /locus_tag="VF686_03275"
     CDS             985..1491
                     /locus_tag="VF686_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8660864.1"
                     /translation="MAQETENNPCITDGVAHQHALTLRRVLDATPEQIWKAWMTPELL
                     QQWFAPHPWTISDVQLDPRPGGVFNFVMHGPDGERFPNSGVFLEAVPNRRWITTDAFT
                     PDWQPAGQPFMVATVELTPRDDGKTDYVATARHWNAETMQQHEAMGFHEGWGQCADQL
                     AELLKKSH"
     gene            1533..2012
                     /locus_tag="VF686_03280"
     CDS             1533..2012
                     /locus_tag="VF686_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8660865.1"
                     /translation="MTFVEVTATRPTLQMQRHFDAPRELVWRAWTNPEVMVLWLGPVE
                     WPAVSATQDLRVGGVWRACLRSPDTGQDLWQGGVYREIVPNERLVFTFKWDESHEDGP
                     PADTLVTIQLSDAPDGGTLMDFTHEGLKSEVSLTGHRHGWTSTFERLEAFLARDTAS"
     gene            2009..2380
                     /locus_tag="VF686_03285"
     CDS             2009..2380
                     /locus_tag="VF686_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660866.1"
                     /translation="MRTSPFVWSGRVLSGVVAGFLALDAALKLAGRPDLLQAGIVLDA
                     PPAFLGSALLAATVLYAVPRTAFWGAGLLSVALSGSIAALAAAEDPSRLLFAVYVAIL
                     MWIGLFLRKPGLGPVLMRAVR"
     gene            2417..2857
                     /locus_tag="VF686_03290"
     CDS             2417..2857
                     /locus_tag="VF686_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8660867.1"
                     /translation="MKIVTSLSFRGQCREAFEFYAKVLGGKITAAMPYSDGPPDMPVG
                     EQYKDWLMHCWMDVGDQAIMGADMDVEWAPNIEKPKNGFDVSLHTDDAAEARRWYDAL
                     VEGGKPTMPFAETFWSPGFGGLIDRFGVPWMVNTIPSADWKPGQ"
     gene            2854..3252
                     /locus_tag="VF686_03295"
     CDS             2854..3252
                     /locus_tag="VF686_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8660868.1"
                     /translation="MKASPSVWTGRVLSGLFVLFMLAASITPKFLMAGMVAEQNLDPL
                     GWPAKYLLLIAVIELICTVLYVIPRTSILGAVLMTGLLGGAIATNLRVEMPMFSHTLF
                     GVYLGLVMWGGLWLRDRRLRGLFPIERSAA"
BASE COUNT          556 a         1108 c         1073 g          553 t
ORIGIN      
        1 tccgaatact cgtggccctg ctccgtctcg acatagtcgg gcccgggtcc cggcccaagg
       61 tgccaatagg tccagctctg acccggaatg gtgaagccca tgtcgatgag ggcctgacaa
      121 acggtggcga cgatgtggtg ggcgccgtcc tcattgccgg tgatcaccac gccggcgacc
      181 ttgccgaagg cgacagggcg gccgctgtcg tccgtttcgc cgaacagggc gtccatccgc
      241 tcgagcgcgc gcaggcagac gctggacatc tggcccagcc aggtcggcgt ggcgaagacg
      301 agaatgtcgg cggccatgat cttcgcatgg acggccggcc agtcgtcgcc ctcaccctga
      361 tccgtcttca cgccgggctt caggttcaga tccacgaggc ggatcatctc gacctcggcc
      421 ccgcccttct cgagttccgc gaggacgacc cgggccaacg cctccgtgct ggaggtctcg
      481 ggcgacggct tgagcgtgcc gttgaggatc agggctttca tcggtctcgc tcacggctga
      541 gccagaccaa cccggcgcga ccggagacgt tccggacatt ttatttaacc gggcggttga
      601 caacgatatc ggaccggttc atttaaccta caagttagat acgaagcagg cccatgcaga
      661 ccgatcctct cagcgccaaa ttcgccgccc tggccgaccc gacccgtcgg gccatcctgg
      721 cgcggctgtg cgaaggcgag gccagcgtca acgacctggc cgaaccgttc gacatgagcc
      781 tgccggccgt gtcgaaacat ctgaaggtgc tggagaaggc cgggctgatc agccggggcc
      841 gcgaggccca gtggcgcccc gcccggctgg aaccgatggg catgaagggc atcgccgagt
      901 ggctggaaca ctaccgccgc tactgggacg ccagcttcga ccgcctcgac gcctacctca
      961 agaaaatcca gaaaggggac cccaatggcc caagaaactg agaacaatcc ctgcatcact
     1021 gacggcgtgg cgcaccagca cgccctgacc ctgcgccgcg tcctggacgc cacgccggag
     1081 cagatctgga aggcgtggat gacgccggaa ctgctgcagc agtggttcgc gccccacccg
     1141 tggacgatct cggacgtgca gctcgacccc cgtcccggcg gcgtcttcaa cttcgtcatg
     1201 cacggcccgg acggcgaacg cttccccaac agcggcgtct tcctcgaggc ggttccgaac
     1261 cgccgctgga tcacgacgga cgccttcacg ccggactggc agccggccgg ccagccgttc
     1321 atggtcgcga cggtcgaact gaccccgcgg gacgacggca agaccgacta tgtcgccacg
     1381 gcgcgccact ggaacgccga gacgatgcag cagcacgaag ccatgggctt ccatgaaggc
     1441 tggggccagt gcgccgacca gctggccgaa ctgctcaaga agtcccactg acctccccgg
     1501 ccttcgcccg gacccgttga agagcccgca cgatgacctt cgtcgaagta accgcgaccc
     1561 gcccgaccct gcagatgcag cgccatttcg atgcgccgcg cgagctggtg tggcgggcct
     1621 ggacgaaccc ggaggtcatg gtgctgtggc tgggtccggt cgaatggccg gcggtcagcg
     1681 ccacccagga cctgcgggtc gggggcgtct ggcgggcatg cctgcggtcg cccgacaccg
     1741 gtcaggacct gtggcagggc ggcgtctacc gcgaaatcgt gccgaacgag cggctggtct
     1801 tcaccttcaa atgggacgag tcgcacgagg acggtccgcc ggccgatacc ctggtgacga
     1861 tccagctcag cgacgcgccg gacggcggca ccctgatgga cttcacccat gaggggctga
     1921 agtcggaggt cagcctgacc ggtcacaggc atggatggac cagcacattc gagcggctgg
     1981 aggccttcct ggccagagat acggcgtcat gaggacctcg cccttcgtct ggtccggccg
     2041 ggtgctgagc ggcgtggtcg cgggcttcct ggcgctggac gcggcgctga agctggcggg
     2101 acggcccgac ctgcttcagg ccggaatcgt tctggacgcg ccgcccgcgt ttcttggtag
     2161 tgcgctgctg gccgcgaccg tactttacgc cgtgccccgc accgccttct ggggcgccgg
     2221 gttgctgagc gtggccttga gcggctcgat cgccgccctg gccgccgcgg aggatccgtc
     2281 ccgtctgctg ttcgcggtct acgtcgcgat cctgatgtgg atcggtctct ttctgcgcaa
     2341 gcccggcctc ggtccggtcc tgatgcgcgc cgtccgatag ttccgttccg actcaaacct
     2401 tcaagggaga acgaccatga agatcgtgac cagcctcagc ttccgcggcc agtgccgtga
     2461 agccttcgaa ttctacgcca aggtcctcgg cggcaagatc accgccgcca tgccctacag
     2521 cgacggacca ccggacatgc cggtgggcga gcagtacaag gactggctga tgcattgctg
     2581 gatggatgtc ggcgatcagg ccatcatggg cgccgacatg gatgtcgaat gggcccccaa
     2641 tatcgaaaag cccaaaaacg gcttcgacgt cagcctgcac accgacgacg cggccgaagc
     2701 ccgtcgctgg tatgacgccc tggtcgaggg cggcaagccg accatgccgt tcgccgagac
     2761 cttctggtcg cccggtttcg gcggcctgat cgaccggttc ggcgtgccgt ggatggtcaa
     2821 caccattccg tcggccgact ggaagcccgg ccaatgaaag cctcgccctc cgtatggacc
     2881 gggcgggtgc tgagcgggct gttcgtcctg ttcatgctcg ccgcctcgat cactccgaaa
     2941 ttcctcatgg ccggcatggt cgcagagcag aatctggatc cgctcggctg gccggcgaaa
     3001 tacttgctgc tgatcgcggt gatcgaactg atctgcaccg tgctgtacgt cattccccgc
     3061 accagcattc tgggcgcggt gctgatgacg ggactgctgg gcggggccat cgccaccaat
     3121 ctgagggtcg agatgccgat gttcagccac accctgttcg gcgtctatct cgggctggtg
     3181 atgtggggcg gtctgtggct gagggatagg cggctgcgcg gcctcttccc gatcgaacgg
     3241 agcgccgcat gaccgtcgcc gcctccatcc ctgaacacct gacccggctc
//
LOCUS       DASVVN010000212         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_322080,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000212 DASVVN010000000
VERSION     DASVVN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322080"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VF686_03300"
     CDS             complement(<1..173)
                     /locus_tag="VF686_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8660869.1"
                     /translation="MTALQTVERARELRRRMTPPEARLWVCLRGKRLAGLKFRRQHPI
                     GPYVLDFYCASAKL"
     gene            complement(284..1075)
                     /gene="mazG"
                     /locus_tag="VF686_03305"
     CDS             complement(284..1075)
                     /gene="mazG"
                     /locus_tag="VF686_03305"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX8660870.1"
                     /translation="MTPIDRLTAIMARLRDPVGGCPWDVEQTFATIAPYTLEEAYEVA
                     DAIERGDMDELRSELGDLLFQVVFHARMAEEQGLFAFDDVANAIADKLERRHPHVFGD
                     EAAKQDGAAQKARWEDIKAAERVAKAQHGVLDDVPVGLPALARAAKLTKRAARVGFDW
                     PSTDEVFDKLDEEVAELRVEIAAGDLDKARDEVGDLLFVVANLARKLGVEPEDALRGA
                     NAKFVRRFGFIEAELAKDGRSPEQSDLAEMDGLWDAAKARERLSS"
     gene            complement(1072..>1770)
                     /locus_tag="VF686_03310"
     CDS             complement(1072..>1770)
                     /locus_tag="VF686_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660871.1"
                     /translation="KKTVRKLLFADLLLGVAPGITGSLLFFFFGQIKGYDHTQASLFM
                     LFYFVAGLVGAPFWAWLATRIGKDKALATASLIFAVFYIGATLVPGGNFALTAVAMFI
                     AGLPYAAGLFLLRAMMADAGDEVRLDTGVDRTGLMFSILSATTKIGHVVALIPYLVLQ
                     AVGFRAIPGVGGNSEMSLLTLQILFILVPGLLLAAAALVLKGYPLTPARHDEIRKALE
                     VRDGATTEPAAEPA"
BASE COUNT          288 a          604 c          595 g          283 t
ORIGIN      
        1 agcttcgccg acgcgcagta gaagtccaga acgtatgggc cgatcggatg ctgacggcgg
       61 aacttcaggc ccgccagtcg cttgccccgc aggcagaccc atagccgcgc ttccggaggc
      121 gtcatgcgcc gacgaagttc gcgggctctc tcaactgtct gcaacgccgt cattgcccca
      181 ccaccacttc gtggtccccc tccccatggc ttcgccacag ggaggagacg gagggtcagc
      241 ttgtccgcag cggatcggcg gcgctagcga atacagcctc agattatgaa ctcagtctct
      301 ctcgcgcctt cgcggcgtcc cacagcccgt ccatctcggc cagatcggac tgctccggcg
      361 accgtccatc cttcgccagt tccgcctcta taaatccgaa ccgccggacg aatttcgcat
      421 tcgccccacg cagcgcgtcc tcgggctcga ctcccagctt ccgcgccaga ttggcgacca
      481 cgaacagcag gtcgccgacc tcgtcgcggg ccttgtcgag gtcgccggcg gcgatttcga
      541 cgcgcagttc tgcgacctcc tcgtccagct tgtcgaagac ctcgtcggtc gagggccagt
      601 cgaagcccac gcgggcggcg cgtttggtca gtttggcggc acgggccagc gcgggcaggc
      661 cgacggggac gtcgtccagc acgccgtgct gggctttggc gacgcgttcg gcggccttga
      721 tgtcttccca gcgggccttc tgggccgcgc cgtcctgctt ggcggcctcg tcgccgaaga
      781 cgtgagggtg gcggcgttcc agcttgtcgg cgatggcgtt ggccacgtcg tcgaaggcga
      841 acagcccctg ttcctcggcc atgcgggcgt ggaagacgac ctggaacaac aggtcgccca
      901 gctcgctgcg cagctcgtcc atgtcgccgc gctcgatggc gtcggcgacc tcataggcct
      961 cttccagcgt gtagggggcg atggtggcga aggtctgctc cacgtcccac gggcagccgc
     1021 cgacggggtc gcgcagccgg gccatgatgg cggtcaggcg gtcgatgggg gtcatgcggg
     1081 ctccgcggcc ggttcggtcg tcgcgccgtc gcggacctcg agcgccttgc ggatctcgtc
     1141 gtggcgcgcg ggggtcagcg gatagccctt cagcacaagc gccgccgccg ccagcagcag
     1201 gcctggcacg aggatgaaga ggatctgcag cgtcagcagc gacatctcgc tgttgccccc
     1261 gacgccggga atggcccgga agccgacggc ctgcagcacc agatagggga tcagcgccac
     1321 gacgtggccg atcttggtcg tggctgacag gatggagaac atcaggccgg tgcggtccac
     1381 gcccgtgtcc agccgaacct cgtcgcccgc gtcggccatc atggcccgca gcaggaacag
     1441 gccggcggca tatggaagcc cggcgatgaa catggcgacg gccgtcaggg cgaaattgcc
     1501 gcccgggacc agggtcgcgc cgatgtagaa gaccgcgaag atcaagctgg cggtcgccag
     1561 cgccttgtcc ttgccgatcc gcgtcgccag ccaggcccag aacggcgccc cgaccagtcc
     1621 ggccacaaaa tagaacagca tgaacagcga ggcctgggtg tggtcgtagc ccttgatctg
     1681 gccgaagaag aagaacagca gcgagccggt gatgcccggc gcgacgccca gcagcaggtc
     1741 ggcgaacagc agtttgcgca ccgtcttttt
//
LOCUS       DASVVN010000213         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_191476,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000213 DASVVN010000000
VERSION     DASVVN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191476"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VF686_03315"
     CDS             complement(<1..513)
                     /locus_tag="VF686_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8660872.1"
                     /translation="MTRKSIQYMGAASALALAVSLACAGGVSAQVTTSTIRGSVTDDG
                     VAEAGAAIVARDTSSGFTTRSTANANGGYVLSGLRPGTYEVTVTTADGETASDVVTIG
                     VGQVGDLDLAVGAAVAADAGASDLGEPLQTAEETTAAATAAGGEPTEVAGVTVVARRL
                     PEVRTPEIATN"
     gene            complement(513..1586)
                     /locus_tag="VF686_03320"
     CDS             complement(513..1586)
                     /locus_tag="VF686_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8660873.1"
                     /translation="MKAATIRDVAERADVSVASVSRVLNGAGPVTEATRAKVLEAADA
                     LQYVPHSGARSLSTSKTQTIGVILPDLYGEFFSELIRGMDIAARSVGYHLIVSSSHDD
                     AEEASAAIRSMRGRVDGLIVLSPHLDAANLAAGLAGRTPILLMNGGADANRPSIVVDN
                     HGGAVQAVEHLIATGRRRIAHISGPTGNLEAETRLAGYLEAMARAGLPTTVVDGAFTQ
                     TSGHDAGVALASGDVRPDAVFAGNDIMAVGAMLALQDAGLRVPEDVAIVGFDDVPIAS
                     LVRPALTTLRIQIAETGRSALERVVRLINAAGDAVADTACEVVRPELVVRPSSQSAAS
                     RTNPSGGRLRADADTASLKGEKS"
     gene            1876..>2873
                     /locus_tag="VF686_03325"
     CDS             1876..>2873
                     /locus_tag="VF686_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX8660874.1"
                     /translation="MTSENTGNYRFPEGFLWGAATAAYQIEGSSMADGAGASIWDRFS
                     HTPGMMLNGDTGDVACDHYNRWREDIDLMTRLNLQAYRFSVSWSRVIPEGRGAVNPKG
                     LDFYDRLVDGLLEKGIEPLCTLYHWDLPAALDDRGGWLNPDIADWFADYGQILFETFK
                     GRVKTWGTINEPWVIVDGGYLHGALAPGHKSAFEAMIAGHNVLRAHGAAVKRFRQVGE
                     GKIGIVLNIEPKYPASDKPEDEAARRRAEAQMNRWFLDPLMGRGYPEELKDVYGEAYR
                     EFPQEDFDLIAQPTDWMGLNWYTRAVPENAPDAWPTRSRPVKQIQHAHTETGWEVYP"
BASE COUNT          467 a          970 c          977 g          459 t
ORIGIN      
        1 gttggtggcg atctcgggcg tgcgcacttc aggcaaccgc cgcgcgacca ccgtcacccc
       61 ggcgacctcg gtcggctccc ctcccgccgc ggtggccgcc gccgtcgttt cttccgccgt
      121 ctgcagcggc tcgcccagat cggaagcgcc ggcgtccgcg gcgacggccg cgccgaccgc
      181 gagatccaga tcgccgacct gaccgacgcc gatggtgacg acgtcggagg cggtttcgcc
      241 atcggccgtg gtcacggtga cttcataggt gccggggcgc aggcccgaca gcacatagcc
      301 gccgttcgca ttggcggtgg accgggtggt gaagcccgac gaggtgtcgc gcgccacgat
      361 cgcggcgccg gcctcggcga cgccgtcgtc cgtcaccgaa ccgcggatcg tcgacgtcgt
      421 gacctgggcg gaaaccccgc cggcgcacgc cagcgaaacc gccagggcga gggcggacgc
      481 ggcccccatg tattgaatgg acttgcgggt catcaggatt tctccccctt cagagatgcc
      541 gtgtcggcgt cggcgcgaag acgcccgccg gatggattgg ttcgggatgc cgccgattgg
      601 cttgacgggc gcacgaccag ttcgggccgg acgacctcgc atgcggtgtc ggcgacggcg
      661 tcgcccgctg cgttgatcag gcggacgacg cgctccaagg cgctgcgtcc ggtttctgca
      721 atctggattc tcagggtcgt cagggcgggc cggaccaggc tggcgatcgg cacgtcgtcg
      781 aagccgacga tggcgacgtc ctcgggcacg cgaaggcctg cgtcctgcag cgccagcatg
      841 gcgccgacgg ccatgatgtc gttgccggcg aagaccgcgt cgggcctgac atcgccgctg
      901 gccagtgcga cgccggcgtc atggcccgac gtctgggtga aggcgccgtc gaccaccgtc
      961 gtcggcaggc cagccctggc catggcctcg agatagccgg ccagccgcgt ctcggcctcg
     1021 aggttgccgg tgggtccgga aatgtgcgcg atgcgccggc ggcccgtcgc gatcaggtgc
     1081 tcgaccgcct gcaccgcccc gccgtggtta tcgaccacga tcgacggccg gttggcgtcg
     1141 gctccgccgt tcatcagcag gatgggggtg cgcccggcca ggcccgcggc cagattggcg
     1201 gcgtccagat ggggcgacag gacgatcagg ccgtcgacgc gtccgcgcat cgaacggatt
     1261 gcggcggagg cctcttccgc atcgtcgtgc gagctggaga cgatcaggtg atagccgacc
     1321 gagcgcgcgg cgatatccat gccgcggatc agttcggaga agaactcgcc gtacaggtcc
     1381 ggcaggatga cgccgatcgt ctgggtcttg ctggtcgaca gactgcgggc gcccgagtgg
     1441 ggtacatact gcagggcgtc ggcggcctcg agcaccttgg cgcgggtcgc ttcggtgacc
     1501 ggccccgccc cgttcagaac gcgtgaaacc gaggccacgg acacgtcggc ccgttcggcc
     1561 acatcgcgga tcgtggcggc cttcatgccg ccgccgtcgc gcagattcgt ggggtcgctg
     1621 tcagcgtcaa aaacatcatc ctccctcggg atttccagtg ccggcccgct tggcgcgggt
     1681 ccgtccgaaa aaaccattcc gaccgattga aagaatgtaa tcggttacgt aaccgattac
     1741 atgacacact agtcaggccg cccgcaagtg ccctagtttg gtcacatgca ggttgtgtcg
     1801 acgggggtac acgcgtaccg accacggcca atctacctca ccagttcgcg ttccagcttt
     1861 tcggggcccc tcaagatgac ttcagaaaac acaggtaact atcggtttcc cgaaggtttt
     1921 ctgtggggcg ccgcaacggc cgcctatcag attgaaggct cgtccatggc cgacggcgcc
     1981 ggtgcgtcga tctgggatcg attcagccac actccgggca tgatgctgaa cggcgacacg
     2041 ggcgacgtgg cctgcgacca ctacaaccgc tggcgcgagg acatcgatct gatgacccgc
     2101 ctgaacctcc aggcctaccg tttctcggtg agctggagcc gggtgattcc cgagggccgc
     2161 ggggccgtca acccgaaggg tctcgacttc tacgacaggc tggtcgacgg ccttctcgag
     2221 aagggcatcg agccgctgtg caccctgtat cactgggatc tgcccgcggc gctggacgat
     2281 cgcggcggct ggctgaaccc ggacatcgcc gactggttcg ccgactacgg tcagatcctt
     2341 ttcgagacgt tcaagggccg ggtgaagacc tggggcacga tcaacgagcc gtgggtgatc
     2401 gtcgacggcg gatatctgca cggcgccctc gcccccggcc acaagtcggc cttcgaggcc
     2461 atgatcgccg gccacaatgt gctgcgcgcg cacggcgccg cggtgaagcg tttccgtcag
     2521 gtgggtgaag gcaagatcgg catcgttctc aacatcgagc cgaaataccc cgcctcggac
     2581 aagcccgagg acgaggccgc gcgcagacgc gccgaggccc agatgaaccg ctggttcctc
     2641 gaccccctga tgggccgcgg ctatccggag gagctgaagg acgtctacgg cgaggcctac
     2701 cgcgagttcc cgcaggaaga cttcgacctg atcgcccagc cgaccgactg gatggggctg
     2761 aactggtaca cccgcgccgt gcccgagaac gcgcccgacg cctggccgac ccgctcgcgc
     2821 ccggttaagc agatccagca cgcccacacc gagaccggct gggaggtcta tcc
//
LOCUS       DASVVN010000214         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_349208,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000214 DASVVN010000000
VERSION     DASVVN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349208"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VF686_03330"
     CDS             complement(<1..1004)
                     /locus_tag="VF686_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HEX8660875.1"
                     /translation="MTAPLGPRLSACGPVVDAASGARAQERLIEAATEGGWASTLQAA
                     WPALAPVFAASPYLFGLARRWPEQLRAVLEEAPDQRLSGILAATEALTGGADDVRAPL
                     RRLKGQLHLLTALCDLGGVWDLNGVTSALSRFADASAQAALRAVAHDQRERGKLVSAP
                     DDPRGPIPGLFGLAMGKHGAFELNYSSDIDISLFWEPEALESALAEGVEPQKFLDRIA
                     HSLSTLMQERTGDGYVFRVDLRLRPDPSSTPPVVAVPFALSYYEGVGQNWERAAFIKA
                     RAMLGDIGAGRDFIRALQPFIWRRSLDFPAILDIQSIKRQIHVHKTGEGLDAAGANLK
                     L"
     gene            complement(1001..2404)
                     /locus_tag="VF686_03335"
     CDS             complement(1001..2404)
                     /locus_tag="VF686_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8660876.1"
                     /translation="MKLPSLLRRTPFRLTLLFLALFAAAASAILAWVYFASAEAARTK
                     AERDVRGEMQVLTGIYDARGLDALNMAVIDRTLTGRYEYLLMDKEGDRITGSLSISPF
                     ETFEGNRQWITFPFTYTLDDGTVVRPQVRGVQVRLAGGEMLFVGASLIDTEAYLARLT
                     QALWAAMGIVLLLGLAGGILVSRNLERSMGRLGKVVTAVQEGDLKARAVVRNSGDELD
                     ELGAGLNVMLDRMEASMASIRHAGDAIAHDLRSPLTRMRAKLEVALMDAEAGKIDGVD
                     AVQLALDEADSLLKTFNTVLAIARLQAAAGRTPDPKVFDAADLAADMAELYEPASEDK
                     GLEFSAEIERGLMVEGNQPFLAQALANIIDNAIKYTPTGGAVMLRARRRSSGEIEFSV
                     TDTGPGVPEEDRERVIERFVRLDNSRTEAGSGLGLSLVGAVMEAHGGRIILDEGPGAY
                     GGFGPGLRVALVLPAAE"
     gene            complement(2414..3085)
                     /locus_tag="VF686_03340"
     CDS             complement(2414..3085)
                     /locus_tag="VF686_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8660877.1"
                     /translation="MRILVVEDDAEAATAMVRGLTEAGHEPTHAVDGAFGLLEAQKGG
                     YDVYVVDRMMPRLDGIGMVETLRKDGDQTPVLFLSALGEVEDRVTGLKAGADDYLVKP
                     YAFSELIARVEALSRRRETGSVATMLKVGDLEMNLISRSVHRSGQEIDLQPREFQLLE
                     FLMRHAGQSVTRTMLLEKVWEYHFDPQTNVIDVHISRLRAKIDKGFDHAMLQTVRGAG
                     YRLEP"
     gene            complement(3662..>3843)
                     /locus_tag="VF686_03345"
     CDS             complement(3662..>3843)
                     /locus_tag="VF686_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8660878.1"
                     /translation="LQVPPGLVILRANNRAVSTPAELKAAVDAARSAGRPGILLFVRL
                     PAGLRSVVLPLIDEE"
BASE COUNT          653 a         1272 c         1294 g          624 t
ORIGIN      
        1 agcttcagat tggccccggc cgcgtccagc ccctcgccgg tcttgtggac gtggatctgg
       61 cgtttgatcg actggatgtc gaggatggcc gggaagtcga ggctgcggcg ccagatgaac
      121 ggctgcaggg cccggatgaa atcacgcccg gccccgatgt cgcccagcat ggcgcgcgcc
      181 ttgatgaagg cggcgcgttc ccagttctgg ccgacgcctt cgtagtagga cagggcgaag
      241 ggcacggcga cgacgggcgg ggtcgaggac ggatcgggac gcagccgcag atcgacgcgg
      301 aagacatagc cgtcgccggt ccgctcctgc atcagcgtcg acagggaatg ggcgatgcgg
      361 tcgaggaatt tctgcggctc gacgccctcg gcgagcgcgg attcgagcgc ctcgggctcc
      421 cagaacaggc tgatgtcgat gtcggaggaa tagttgagct cgaaggcgcc gtgcttgccc
      481 atggcgaggc cgaacaggcc ggggatgggg ccgcgcggat cgtcgggggc ggagaccagc
      541 ttgccccgct cgcgctggtc gtgggccacg gcgcgcaggg ccgcctgggc cgaggcgtcg
      601 gcgaaccggc tgagggcgga ggtgacgccg ttcaggtccc acacgccgcc gagatcgcac
      661 agggcggtca gaagatggag ctgacccttg agccgccgca ggggcgcgcg cacgtcgtcg
      721 gcgccgccgg tcagggcttc ggtggccgcc agaatgccgg aaaggcgttg gtcaggcgcc
      781 tcctccagca ccgcacgcag ttgttcgggc cagcgacgcg cgaggccgaa cagatagggc
      841 gaggcggcga agacgggcgc gagggccggc catgccgcct gcagggtcga cgcccagccg
      901 ccctccgtcg ccgcctcgat caggcgttcc tgcgcgcggg ccccggacgc ggcgtcgacc
      961 acgggtccgc aggcgctcag gcgcgggccc agaggcgcgg tcattccgcc gccggcagga
     1021 ccagagccac ccgcaggccg ggcccgaagc cgccgtaggc gccggggccc tcgtccagaa
     1081 tgatccgccc gccgtgggcc tccatgaccg cgccgacgag ggacaggcca aggcccgagc
     1141 ccgcctccgt gcggctgttg tccagacgca cgaagcgttc gatgacccgc tcgcggtcct
     1201 cttcaggcac gccggggccg gtgtcggtga cggagaattc gatctcgccc gacgagcgcc
     1261 gccgcgcgcg cagcatcacc gcgccgccgg tcggcgtgta tttgatggcg ttgtcgatga
     1321 tgttggccag cgcctgggcg aggaagggct ggttgccctc gaccatcagg cctcgctcga
     1381 tctcggccga gaattccagc cccttgtcct cgctggccgg ttcgtagagc tcggccatgt
     1441 cggcggccag atcggcggcg tcgaacacct ttggatccgg ggtgcggccg gcggcggcct
     1501 gcagccgggc gatggccagc acggtattga aggtcttcag caggctgtcc gcctcgtcca
     1561 gcgccagctg gaccgcgtcg acgccgtcga tcttgccggc ctcggcgtcc atcagggcga
     1621 cttccagctt ggcccgcatc cgggtcaggg gggagcgcag gtcgtgggcg atggcgtcgc
     1681 cggcgtggcg gatcgaggcc atcgaggcct ccatccggtc cagcatgacg ttcaggccgg
     1741 cgcccagttc gtccagttca tcgcccgagt ttctgaccac ggcgcgggcc ttcagatccc
     1801 cttcctgcac ggcggtgacg accttgccca gccgacccat ggaccgttcg agattgcggc
     1861 tgaccagaat ccctccggcc agccccagca gcagaacgat gcccatggcc gcccacaggg
     1921 cctgggtcag gcgcgccaga taggcttcgg tgtcgatcag cgacgccccg acgaacagca
     1981 tctcgccgcc cgccagacgc acctgcactc cgcggacctg cggccggacc accgtcccgt
     2041 catccagggt gtaggtgaag gggaaggtga tccactgtcg gttcccctcg aaggtttcga
     2101 agggggagat gctgaggctg ccggtgatcc ggtcgccctc cttgtccatc agcagatatt
     2161 cgtagcggcc ggtcagggtg cggtcgatca ccgccatgtt cagggcgtcc agtccccgcg
     2221 cgtcatagat gcccgtaagc acctgcatct cgccgcgcac gtcgcgctcg gccttggtgc
     2281 gcgccgcttc ggccgaggcg aaatagaccc aggccaggat cgcgctggcc gccgccgcga
     2341 acagggcgag gaacagcagc gtcagccgga agggcgtgcg gcgaaggagg gagggaagtt
     2401 tcacgggctg gagtcagggc tccagccggt agcccgcccc gcgcaccgtc tgcagcatgg
     2461 cgtgatcgaa gcccttgtcg atcttggcgc gcaggcggct gatgtggacg tcgatgacat
     2521 tggtctgggg gtcgaaatga tattcccaga ccttctccag cagcattgtg cgggtcaccg
     2581 actggccggc gtggcgcatc aggaattcca gcagctggaa ttcgcgcggc tgcaggtcga
     2641 tctcctggcc cgagcggtgc accgagcggg agatcaggtt catctccaga tcgccgacct
     2701 tgagcatggt ggcgaccgaa ccggtttcgc ggcggcgcga cagggcctcg acgcgggcga
     2761 tcagttcgga gaaggcgtag ggcttgacca gatagtcgtc ggcgccggcc ttcaggcccg
     2821 tgacccggtc ctcgacctcg ccgagggccg acaggaacag caccggcgtc tggtcgccgt
     2881 ccttgcgcag ggtctcgacc atgccgatgc cgtcgaggcg gggcatcatc cggtccacga
     2941 catagacgtc gtagccgccc ttctgggcct ccagcagacc gaaggcgccg tcgacggcgt
     3001 gggtcggctc gtgaccggcc tcggtcaggc cccggaccat cgcggtcgca gcctcggcgt
     3061 cgtcctcgac caccagaata cgcatggaat cagcccctca gatgcggaat cgccgccgat
     3121 gttagcgcat cggcggcgga cccgtgagtt aagatgcagt cactctacct taggtaatta
     3181 agtgaaagcc tttccagctg cacatcaaat taaacgtact gaactgtact gataagtgta
     3241 taggtaatct caacttctgc gtcgagagtc tgcatgttta cagtgacggt ctcgcacaca
     3301 ttatagattt cataccccca cgagtagtta actctctccc aactacaaac tctgtctcgt
     3361 gcagctgatg ctgcaaatgc atgatcgggg gcaccaagtc caaccagtcc actgaacaac
     3421 gctattgcgc aagcaatatt catcgcatgc tttttcattt tcacctcccg ttatgccccc
     3481 tccagggaac atctcacact atgcgaatcc accatggact gtaaacccaa aggtgcattg
     3541 cagcccaaaa gtgcatccgg tcgccgctcg aaccgcttct gggatacaag catagtgatc
     3601 ccaaacggcg gcgggccgag cagttgcggc ttgttcaggt gcgagagtcg cccgagtgtc
     3661 gctattcctc gtcgatcaac ggcaaaacga ccgaccgcag ccctgccggc agccgcacga
     3721 acagcagaat gccaggtcgc ccggcgctcc gcgccgcgtc gacagcggcc ttcagttccg
     3781 caggcgtgga aaccgcgcgg ttgttcgccc gcaggatcac caatcccggc gggacctgca
     3841 gcc
//
LOCUS       DASVVN010000215         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_402055,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000215 DASVVN010000000
VERSION     DASVVN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402055"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF686_03350"
     CDS             complement(<1..555)
                     /locus_tag="VF686_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX8660879.1"
                     /translation="MTQAPAYDVCAVGNAIVDVLAPCAPAFLAAQGLTAGSMQLVDAQ
                     QSGALYAAMAAGVEASGGSAGNTVAGVGSFGGRAAYIGKVADDTLGGVFTHDIRAAGV
                     AFETPPLRDGAAAGLATGVCMINVTPDGQRTMCTFLGAANQLAAADIDADLIGASAIV
                     YLEGYLFDPAPARAAFEAAAAAAHA"
     gene            669..1538
                     /locus_tag="VF686_03355"
     CDS             669..1538
                     /locus_tag="VF686_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8660880.1"
                     /translation="MTQVAVLTPDPSDPSYAGQWPGVLERLGEALSLAGVTVAPTPWT
                     EHVADAGALKRFPLVLPLIVWGYHRDHERWMQACATWTEAGLTMLNPPAVLGWNSDKS
                     YLGRLADKGIAIPDTIWIDSPTRGDVDAAFDRFGTDQLVVKPRVSGGAWKTLRLSRGD
                     ALTDAPQGPAMIQPYLPTIETEGETSLLFFGGKLSHVVNKRPVPGEFRVQVQYGGGYT
                     ALPEPPAGALALAERTLAAIGEDLLYARIDMAPDGDGGWLLMEAELIEPDFYLGSAPD
                     RGQAFAEAVRARL"
     gene            1580..1888
                     /locus_tag="VF686_03360"
     CDS             1580..1888
                     /locus_tag="VF686_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660881.1"
                     /translation="MNWLYAIMAPPWDYPEGWFAYLAVPILTSVSLNRLMPRHRLAAV
                     VALSVAVSLFLYFALWTALYGVHSTTAIGVMFALVPYSVVCVLTVGIVRSAQAASPSA
                     "
     gene            complement(1864..2601)
                     /gene="nth"
                     /locus_tag="VF686_03365"
     CDS             complement(1864..2601)
                     /gene="nth"
                     /locus_tag="VF686_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8660882.1"
                     /translation="MKPPKARPKSPVKRPAVMTGAVIPMLGWPPDEDRVEAIFERLSA
                     VMPEPKTELNFSSPYTLVVAVALSAQATDVSVNKATDRLFRVADTPEKMLALGEEGLV
                     PYIASIGLYRGKARNVIALSRIVLEQHGGEVPLNRADLQALPGVGRKTASVVLNELGI
                     EAAIAVDTHVFRVSHRLGLANAATPDKVEAQLFRVVPEEWLPKAHHWLILHGRYTCTA
                     RRPKCSACLISDLCPSRAGLQALGEAA"
     gene            complement(2598..3878)
                     /locus_tag="VF686_03370"
     CDS             complement(2598..3878)
                     /locus_tag="VF686_03370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8660883.1"
                     /translation="MSADRPPSSVDVAIVGAGAAGIAAARALAGATLDVVVLEARDRI
                     GGRAHTIDRDGTGLDMGCGWLHSADENVLAGLADAQGFTVDRTPPPWESQAFNHEMSP
                     AEQAEFRTAFAAFEQRLAEAADRGEEGPASALFDPDCRWNPRIDAISGALNGARFAEV
                     SIRDYDAYRDTGVNWRVAEGYGRLVAQLGDGLPVIADCPVSSFDRSGPILRLQTAKGT
                     VEARAVIVTVPTDLIARETLRFDPPLPALVEAAAGLPLGLASKLHMTVEGAEDFPKDS
                     QLWGRSDTAETGGYHLRPFGRPMIEGWFGGDIARALEAEGEAAFFAFATDELAHLLGS
                     SFRKRIAPIATSMWGDDPWSRGAYSHALPGHADDRARLAAAVENRLFFAGEATSPDFY
                     GTAHGAWMEGERAARQALAALGLDPGPHPGDDGA"
     gene            4001..>4782
                     /locus_tag="VF686_03375"
     CDS             4001..>4782
                     /locus_tag="VF686_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX8660884.1"
                     /translation="MALSPALKKRLPLIVGAVVAVAVVIGGVVWWQGKQRWEATDNAF
                     VAADTTEVSPQIDGYVVEVLVADNQRVEPGQILIRLDDANARAALTQAEANLASVIAA
                     VGNVDARAAQEQALIASRAAGVAQAQAQADLARQQVDRYGRLAEQGWVSQARIQTEQA
                     GARTAQATVAEAQAALVAEQRTAGVLGSTRSQSVAAVEVARAQVEQARIALDRTVIRA
                     PVGGVIGARGVRVGQYVRPGGQILSIVPLGDAYVVANFKETQL"
BASE COUNT          680 a         1673 c         1708 g          721 t
ORIGIN      
        1 cgcgtgggcg gcggcggcgg cggcctcgaa ggcggcccgg gccggggccg gatcgaacag
       61 atagccttcc agatagacga tggccgaggc gccgatcagg tcggcgtcga tgtcggccgc
      121 ggccagctgg ttggccgcgc cgaggaaggt gcacatggtg cgctggccgt cgggcgtgac
      181 attgatcatg cagacgccgg tggcgagacc ggcggcggcg ccgtccctca gcgggggcgt
      241 ctcgaaggcc acgccggcgg cgcggatgtc gtgggtgaag acgccgccca gggtgtcgtc
      301 cgcgaccttg ccgatatagg ccgcgcgccc accgaagctg ccgacgccgg cgaccgtgtt
      361 gccggccgag ccgcccgagg cttccacccc ggccgccatg gcggcgtaga gggcgccgct
      421 ctgttgcgcg tcgaccagct gcatcgagcc ggcggtcaag ccctgggcgg ccaggaaggc
      481 gggggcgcag ggtgcgagaa cgtcgacgat cgcgttgccg acggcgcaga catcataggc
      541 gggggcttgg gtcatggcgc gcgcttagcg ggcctgagcg cgcttgacca gccacaccgg
      601 tctttcctta tgccgggaca aggaaatccg ggttgcgcgc cgccgcgatt cccggtcagg
      661 agccgggcat gacccaagtt gccgtcctga cccccgaccc gtccgatccc tcctacgccg
      721 gccagtggcc cggggtgctg gagcggctgg gcgaggccct gtcgctggcg ggggtgaccg
      781 tcgcgccgac gccgtggacg gagcacgtgg cggacgccgg cgccctgaag cggtttccgc
      841 tggtgctgcc gctgatcgtc tggggctatc atcgtgacca cgagcgctgg atgcaggcct
      901 gcgcgacctg gaccgaagcg gggctgacga tgctgaaccc gccggcggtg ctgggctgga
      961 attcggacaa gagctatctc ggacggctgg ccgacaaggg gatcgccatc cccgacacga
     1021 tctggatcga cagcccgacg cggggcgacg tcgacgccgc cttcgaccgg ttcgggacgg
     1081 atcagctggt ggtcaagccg cgcgtttccg gcggggcgtg gaagacgctg cggctgtcgc
     1141 gcggcgacgc cctgaccgac gccccgcagg gcccggcgat gatccagccc tatctgccga
     1201 ccatcgagac cgagggcgag acctcgctgc tgttcttcgg cggcaagctc agccacgtcg
     1261 tcaacaagcg gcccgtgccc ggcgagttcc gggtgcaggt ccagtacggc ggcggctaca
     1321 cggcgcttcc cgaaccgccg gcgggcgcct tggcgttggc agaacggacg ctcgccgcca
     1381 tcggcgagga ccttttgtac gcccgcatcg acatggcgcc cgacggcgac ggcggctggc
     1441 tgctgatgga ggccgaactg atcgagcccg acttttatct ggggtcggcg ccggaccggg
     1501 ggcaggcttt cgccgaggcc gtgcgcgctc gactataggg atgtaaggtg ggagccgacc
     1561 cgcgacccgg agggcttcca tgaactggct atacgcgatc atggccccgc cctgggatta
     1621 ccccgaggga tggttcgcct atctggccgt gccgatcctc acatccgtct ctctgaaccg
     1681 gcttatgccg cgtcaccgcc tggccgcggt cgtggccctg tccgtcgctg tttccctctt
     1741 cctgtatttc gctctctgga ccgccctgta cggtgttcat tcgacgaccg cgatcggcgt
     1801 gatgttcgcg ctcgtcccct attccgtcgt ttgcgttctc acggtcggaa tcgtccgctc
     1861 ggctcaggcc gcttccccca gcgcctgaag cccggcccgc gacggacaca ggtcgctgat
     1921 cagacaggcg ctgcatttcg gtctgcgcgc cgtgcaggtg tagcggccgt gcaggatcag
     1981 ccagtggtgc gccttgggca gccattcttc cggcacgacg cggaacagct gggcctcgac
     2041 cttgtcgggc gtggcggcgt tggccagccc cagccggtgg gagacgcgga agacgtgggt
     2101 gtccaccgcg atggcggcct cgatcccgag ttcgttcagg accacgctgg cggtcttgcg
     2161 acccacgccg ggcagggcct gaaggtccgc gcggttcagc gggacctcgc cgccgtgctg
     2221 ctccagtacg atccggctga gggcgatgac gttcctggcc ttgccgcgat agaggccgat
     2281 cgaggcgatg taggggacca gcccttcctc gcccagcgcc agcatcttct ccggcgtgtc
     2341 ggcgaccctg aaaagcctgt ccgtggcctt gttgaccgag acgtccgtgg cctgtgccga
     2401 cagggcgacg gcgacgacaa gggtgtaggg gctggagaaa ttcagctccg tcttcggctc
     2461 gggcatgacc gccgacagcc gttcgaagat ggcctcgacc cgatcctcgt cgggcggcca
     2521 gccgagcatg gggatgaccg cgccggtcat cactgcgggg cgtttcaccg gggatttcgg
     2581 gcgggccttc gggggcttca tgccccgtcg tcgccggggt gcgggccggg gtcaagcccg
     2641 agcgccgcca gcgcctgacg ggcggcgcgc tctccctcca tccaggcgcc gtgggccgtg
     2701 ccgtagaagt ctggcgaggt cgcctcgccg gcgaaaaaca gccggttctc gacggcggcc
     2761 gccagacgcg cccggtcgtc ggcgtgaccc ggcagggcgt gggaataggc gccccgcgac
     2821 cacggatcgt cgccccacat cgaggtggcg atcggcgcga tgcgtttgcg gaacgacgag
     2881 cccagcagat gggccagttc gtccgtggcg aaggcgaaga acgccgcctc gccctcggcc
     2941 tcgagcgccc gcgcgatatc gccgccgaac cagccctcga tcatcggccg tccgaagggg
     3001 cgcaggtggt agccgccggt ctcggcagtg tctgaccgac cccacagctg gctgtccttg
     3061 ggaaagtcct ccgcgccctc gaccgtcatg tgcagcttgg acgccagtcc cagcggcagg
     3121 ccggcggcgg cctcgaccag cgccggcagg ggcgggtcga aacgcagcgt ctcgcgggcg
     3181 atcaggtccg tcggcacagt gacgatcacg gcgcgcgcct cgaccgtccc cttcgccgtc
     3241 tgcagccgca agatcggccc ggagcggtcg aaagaggaga cggggcagtc cgcgatcacg
     3301 ggcaggccgt cgccgagctg ggcgaccagc cggccatatc cctcggccac ccgccagttc
     3361 accccggtgt cgcgataggc gtcgtagtcg cggatcgaga cctcggcgaa gcgcgcgccg
     3421 ttcagggcgc cggagatggc gtcgatgcgc ggattccagc ggcagtccgg gtcgaacagg
     3481 gccgaggccg gtccctcctc gccgcggtcg gcggcctcgg ccagccgctg ctcgaaggcg
     3541 gcgaaggcgg tgcggaattc cgcctgttcg gccggcgaca tctcgtgatt gaaggcctgg
     3601 ctttcccacg gcggcggggt gcggtcgacg gtgaagccct gcgcgtccgc caacccggcc
     3661 agcacattct catcggccga atgcagccag ccgcagccca tgtcgagccc ggtcccgtcg
     3721 cggtcgatcg tatgcgcccg tcctccgatg cgatcccgcg cctccagcac gaccacgtcc
     3781 agcgtggcgc ccgccagagc ccgggcggcg gcgatgccgg cggcgcccgc cccgactatg
     3841 gcgacatcga cggaggacgg cgggcgatct gcggacatca cgaactccaa acgatcggaa
     3901 acaagacgtg ttgcctcgcc gaaacaagcg ctgttgattt ttggacagta tcgtccattt
     3961 taacgccgtc ctccccgaca cccttccttc gagcagtccc atggccctgt cgcccgcgtt
     4021 gaagaaacgc cttcccctga tcgtcggcgc cgtcgtcgcc gtggctgtcg tcatcggcgg
     4081 cgtggtctgg tggcagggca agcagcgttg ggaggcgacc gacaatgcct tcgtcgccgc
     4141 cgacaccacc gaggtcagcc cccagatcga cggctatgtc gtcgaggtgc tggtggccga
     4201 caaccagcgt gtcgagccgg gccagatcct gatccgcctc gacgacgcca acgcccgggc
     4261 ggccctgacc caggccgagg ccaatctggc ctcggtgatc gccgccgtcg gaaacgtcga
     4321 cgcccgcgcg gctcaggaac aggccctgat cgcctcgcgc gccgccggcg tggcgcaggc
     4381 gcaggcccag gccgatctgg cccgccagca ggtcgaccgc tacggcaggc tggccgagca
     4441 gggctgggtc tcgcaggccc gcatccagac cgaacaggcc ggcgcccgca cggcgcaggc
     4501 gacggtcgcc gaggcgcagg ccgccctggt cgccgagcag cgcaccgccg gcgtgctggg
     4561 ctcgacccgc agccagagcg tggccgccgt cgaggtcgcc cgcgcccagg tcgaacaggc
     4621 gcgcatcgcg ctggaccgca ccgtcatccg cgctccggtc ggcggcgtta tcggcgcgcg
     4681 cggcgtccgc gtcggccagt atgtccgtcc gggtggccag atcctgtcga tcgtgccgct
     4741 gggcgacgcc tatgtggtcg ccaacttcaa ggagacccag ct
//
LOCUS       DASVVN010000216         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_322183,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000216 DASVVN010000000
VERSION     DASVVN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322183"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF686_03380"
     CDS             complement(<1..255)
                     /locus_tag="VF686_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX8660885.1"
                     /translation="MTRANAPRYQSGFGNHFATEAAPGALPEGRNSPQKTPMGLYAEQ
                     LSGTAFTAPRSENRRSWLYRMRPSAEHPPFKPYKGAKLFAP"
     gene            complement(304..1422)
                     /locus_tag="VF686_03385"
     CDS             complement(304..1422)
                     /locus_tag="VF686_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8660886.1"
                     /translation="MPLILTEEQTMLKEAADGFLNEHAPITHLRKLRDSKDADGVSRD
                     LWRAFGEMGFAGILIPEEHGGSGLGAVEAGVMAEALGRTLTPSPFLGSGVLSATVLKG
                     GSAEQQAAWLPRIAAGEAIMSLAVDEGPKHAPARIATTAERSGNGFRLNGAKGFVLDG
                     HVANALIVAAQTEGGLTLFLVDPKTAGVAVERTVMVDAHNAARVTLTDVEVDADAVIG
                     AVDGGEGLLEGALNLGRACAAASLNGAGDQAFQTTLAYLRTRKQFGKLIGEFQALQHR
                     AAHLYSEIEIARAATAGALNALDAGREDAALVVSVAKAKAGRVAELAVQEAVQMHGGV
                     GMTDEFDVGLFMKRVRVLNELLGDAGFHQERMARAQGF"
     gene            complement(1422..>1705)
                     /locus_tag="VF686_03390"
     CDS             complement(1422..>1705)
                     /locus_tag="VF686_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660887.1"
                     /translation="RRMRAAWWVIAIFVWLLSAVFSQQVAGTLYMFGSLDHPTLQVRL
                     ILWAPVVALGVLGVAAILRRKAVWLPALLLLSPALQAVGLVLAKLDPVT"
BASE COUNT          271 a          662 c          522 g          250 t
ORIGIN      
        1 gggcgcgaac agcttggccc ccttgtaagg cttgaacggc gggtgctcgg cggacggccg
       61 catccggtac agccacgacc gccggttctc ggaccgcggc gcggtgaacg ccgtccccga
      121 cagttgttcc gcatacagac ccatcggcgt cttctgcggc gagttccgcc cctcgggcag
      181 ggccccgggc gcggcctcgg tcgcgaaatg attgccgaag ccggattggt atcgaggcgc
      241 gttggcgcga gtcatgctgc gtgtctcccg tgggagaggt ttagggcgcc gggccgccga
      301 ccgctagaac ccctgcgccc gcgccatccg ctcctgatgg aagcccgcat cgcccagcag
      361 ttcgttcagc acccgcaccc gcttcatgaa aaggccgacg tcgaactcgt cggtcatgcc
      421 gacgccgccg tgcatctgca ccgcctcctg caccgccagc tcggcgacgc gcccggcctt
      481 ggccttggcg acggaaacca ccagagccgc atcctcgcgt ccggcgtcca gcgcattcag
      541 cgcccccgcc gtcgccgccc gcgcgatctc gatctctgaa tacagatgcg ccgcccggtg
      601 ctgcagcgcc tggaattcgc cgatcagttt cccgaactgc ttgcgcgtcc gcagatacgc
      661 cagcgtcgtc tggaaggcct gatcccccgc cccgttcagc gacgccgccg cgcacgcccg
      721 gcccagattc agcgcccctt ccagcagccc ctcaccgccg tccactgcgc cgatcacggc
      781 gtccgcatcg acctcgacgt cggtcagcgt cacccgcgcc gcattgtgcg catcgaccat
      841 gacggtccgc tcgaccgcca cgcccgccgt cttcggatcg accaggaaca gcgtcagccc
      901 gccttcggtt tgcgccgcca cgatcagggc gttcgccaca tggccgtcca gcacgaagcc
      961 cttggcgccg ttcagccgga agccgttgcc cgagcgctcc gccgtcgtcg cgatccgcgc
     1021 cggcgcatgc ttgggcccct cgtcaaccgc cagcgacatg atcgcctcgc ccgctgcgat
     1081 ccggggcagc caggctgcct gctgttccgc cgacccgccc ttcagcactg tcgccgacag
     1141 cacgcccgaa ccgaggaagg gcgacggcgt cagcgtccgt cccagcgcct cggccatcac
     1201 cccggcctcg accgcgccca ggcccgagcc gccgtgttcc tcgggaatga ggattccggc
     1261 gaaccccatc tccccgaacg cccgccacag atcgcgcgac accccgtccg catccttgct
     1321 gtcgcgcaac ttcctcaagt gggtgatcgg cgcgtgctcg ttcaggaaac cgtcggcggc
     1381 ctccttcagc atcgtctgct cttcggtcag gatcagcggc atcaggtcac cgggtcaagt
     1441 ttcgccaaca caaggccgac ggcctgcagg gcgggagaaa gaagaagcag ggcgggcagc
     1501 cacaccgcct tgcgacgcag gatcgcggcg accccgagca cgccgagcgc gacgacaggg
     1561 gcccagagga tcaagcggac ctgaagcgtc ggatggtcga gcgatccgaa catgtacagc
     1621 gtgccggcga cctgctggct gaaaacggcg ctcagcagcc agacgaagat cgcgatcacc
     1681 caccaggccg cgcgcatcct acgcc
//
LOCUS       DASVVN010000217         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_454789,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000217 DASVVN010000000
VERSION     DASVVN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454789"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="argF"
                     /locus_tag="VF686_03395"
     CDS             <1..875
                     /gene="argF"
                     /locus_tag="VF686_03395"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX8660888.1"
                     /translation="KRRKRARGDRPKGAPDDDAPAAGRVLGMIFEKSSTRTRYSFDVA
                     MRQLGGSAIVTHASDLQLGRGETVDDTARVLSRMVDAVMIRAERHEDMKRFAHSSSVP
                     VINGLTDRSHPCQILADLMTYEEHRGPVEGRTIAWIGDGNNVCASFIHASAFFGYTLK
                     IACPPEYHPDLMDLAKAAERGAKVTMTADPREAAVGADCIVTDTWISMGDEDYETRYQ
                     AFEPYQVDNAIMDLAAKDAVFLHCLPAHRGEEVTDAVMDGPASLVWDEAENRIHAQKA
                     VLLWCWGLVPGQVR"
     gene            985..>1640
                     /locus_tag="VF686_03400"
     CDS             985..>1640
                     /locus_tag="VF686_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX8660889.1"
                     /translation="MTDTLSDNLVAAFQIEGWPVRGRIVRLGDVVDDILSRHDYPEPV
                     ANLLGEACALAALVGSSLKFDGKLIVQAQGDGPVGYVVADYDTSGSLRGYCRFDAERV
                     AAASQGFARPGAKTLLGDGVFVMTIDQGPQRDRHQGITAIEGETLALCAEQYFAQSEQ
                     VPTRVRLAVGELDVGDGANWRAGGAMVQNIAEDEARGATRDAWEKAEALFSTTGDDEL
                     "
BASE COUNT          265 a          608 c          550 g          217 t
ORIGIN      
        1 ccaagcggag gaagcgcgcc cgcggcgacc gtcccaaggg cgcgcccgac gacgacgccc
       61 cggcggccgg ccgcgtgctg ggcatgatct tcgagaagag ctccacgcgg acccgctatt
      121 ccttcgacgt ggccatgcgt cagctcggcg gctcagccat cgtcacccat gcctcagacc
      181 tccagctcgg ccgcggcgaa accgtggacg acaccgcccg cgtgctgtcg cgcatggtcg
      241 acgcggtgat gatccgcgcc gagcggcacg aggacatgaa acgcttcgcc cattcctcgt
      301 ccgtgccggt gatcaacggc ctcaccgacc gcagccaccc ctgccagatc ctcgccgacc
      361 tgatgaccta cgaggaacac cgcggccccg tcgaaggccg taccatcgcc tggatcggcg
      421 acggcaataa cgtctgcgcc agcttcatcc atgcctcagc cttcttcggc tacaccctga
      481 aaatcgcctg cccgccggag taccacccgg acctgatgga cctggccaag gcggccgaac
      541 gcggcgccaa ggtgacgatg acggccgatc cgcgcgaggc ggcggtcggg gccgactgca
      601 tcgtcaccga cacctggatc tcgatgggcg acgaggacta cgagacccgc taccaggcct
      661 tcgagcccta ccaggtggac aacgccatca tggacctggc ggccaaggac gcggtgttcc
      721 tgcactgcct gcccgcccac cgcggcgagg aggtcaccga cgcggtgatg gacggccccg
      781 cctcgctggt ctgggacgag gccgagaacc gcatccacgc ccagaaagcc gtcctcctct
      841 ggtgctgggg cctcgtgccg gggcaggtgc ggtaggccgc ccacgggctg gaaggcgggg
      901 ctcggctccc tatctttcgg ggcccgccca ccgcatcatc gtacaaacag cgcgagtcga
      961 gccgcgcgca caggacgcgt tcccgtgacc gacacccttt ccgacaacct ggtcgccgcc
     1021 ttccagatcg agggctggcc cgtgcgcggg cgcatcgtgc gcctgggcga cgtggtcgat
     1081 gacatcctga gccgccacga ctaccccgag ccggtggcca acctgctcgg cgaggcctgc
     1141 gcgctcgccg ccctggtcgg ctccagcctc aagttcgacg gcaagctgat cgtgcaggcg
     1201 cagggcgacg ggccagtggg ctacgtcgtc gccgattacg acacctccgg cagcttgcgc
     1261 ggctactgcc gcttcgacgc cgagcgggtg gcggccgcga gccagggctt cgcccgcccc
     1321 ggggccaaga ccctgctcgg cgacggcgtg ttcgtgatga ccatcgacca gggcccgcag
     1381 agggaccgcc accagggcat caccgccatc gagggcgaga cgctggcgct ctgcgccgag
     1441 cagtacttcg cccagtccga gcaggtgcca acccgcgtcc gcctggccgt gggcgagctc
     1501 gacgtcggcg acggcgccaa ctggcgggcc ggcggcgcca tggtgcagaa catcgccgag
     1561 gacgaggcgc gcggcgccac gcgcgacgcc tgggagaagg cggaggcgct gttctccacc
     1621 accggggacg acgagctgat
//
LOCUS       DASVVN010000218         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_377033,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000218 DASVVN010000000
VERSION     DASVVN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377033"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF686_03405"
     CDS             complement(<1..597)
                     /locus_tag="VF686_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8660890.1"
                     /translation="MLQIALLGPLLLGLLALSGIGHRWVDVLFQFTAPVLIATVTLTA
                     FLAGARMWAATLHALAAIVVLALAVAPQWFPASASPRPGAPIVRLYSANLQALNTDVD
                     SILLSLIAANADVVVLIEVGDAPAAQLDRILADHPYRIASPRIDRPSGAARSVIASRW
                     PLTAIRDRPDGLHAVGATVRTPVGPVNVLGVHLTRPWPF"
     gene            714..1187
                     /locus_tag="VF686_03410"
     CDS             714..1187
                     /locus_tag="VF686_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX8660891.1"
                     /translation="MARRSVADIEKIWSNVEGVKKLSDRAVGIGPFGIGLDGLLTWIP
                     VVGTVYSVGAAGWLLIQAGKARATPSTLMRMVGYLGLDSATTAVGEVIPFAPDVVDFF
                     FPGHLMAAKALQKDIESTHWVEASEREARASGEHERHVADMRRNPKLRRIVYLHD"
     gene            1483..1803
                     /locus_tag="VF686_03415"
     CDS             1483..1803
                     /locus_tag="VF686_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8660892.1"
                     /translation="MTIARNRAIDRLRSGAATRRSTPIDDAFDVADDGPSPAQALETG
                     DDVRRLNDCLGELDDRTGGAIRTAFIEGVTYDALASRENVPLGTMKSWIRRGLMRLRA
                     CLES"
BASE COUNT          330 a          630 c          671 g          271 t
ORIGIN      
        1 gaacggccat ggacgggtca ggtggacgcc cagcacattc accggcccca ccggcgtccg
       61 gaccgtggca ccgaccgcgt gcaggccgtc gggccggtcg cggatcgccg tcagcggcca
      121 gcgcgaggcg atcaccgaac gcgccgcgcc gctgggacgg tcgatgcggg gactggcgat
      181 ccggtacgga tgatccgcca gaatccggtc cagctgcgcc gcgggtgcgt cgcccacctc
      241 gatcagaacg acgacgtcgg cgttggcggc aatgagcgac agcaggatcg agtccacgtc
      301 cgtgttcagg gcctgcagat tggcggaata caggcgcacg atcggggcgc ccgggcgagg
      361 ggaggccgag gcgggaaacc attgcggcgc gaccgccagg gccagcacca cgatcgccgc
      421 cagcgcgtgc agggtcgccg cccacatccg ggcgccagcc aggaacgccg tcagcgtcac
      481 ggtcgcgatc aggaccggcg ccgtgaactg gaacagcaca tccacccacc ggtgcccgat
      541 ccccgacagc gccagcaacc ccagcagcag cgggccaagc aaggcgatct gaagcaaggt
      601 ctcgatccgc cgcccccgcg cccacaggcg gccgggacgc agtccttgcc tttcagggtg
      661 aacggggttc gtcatggcgc gttacggtct cgcttaactg gagaggggca tccatggcca
      721 gacgatccgt cgcggatatc gagaagatct ggtccaatgt cgagggcgtc aagaaactgt
      781 cggaccgtgc cgtgggcatc ggcccgttcg gcatcggact ggacggtctg ctgacctgga
      841 tcccggtcgt gggcacggtc tacagcgtcg gcgccgccgg gtggctgctg atccaggcgg
      901 gcaaggcccg ggcgacgcct tccaccctga tgcgcatggt cggctatctg ggactggaca
      961 gcgccaccac ggcggtcggg gaggtgatcc ctttcgcgcc agatgtggtc gacttcttct
     1021 tccccggaca cctgatggcg gccaaggcgc tgcagaagga tatcgagagc acccactggg
     1081 tcgaggcgag cgagcgcgag gcgcgcgcct ctggcgagca cgagcggcat gttgcggaca
     1141 tgcggcggaa tccgaagctg cgccgaatcg tctacctgca tgactgagcg cttgacgaag
     1201 caggtcgtgc gccgccagtt gccacccatg gtttgccgga cgcccgtatg agttccatcc
     1261 tcacccgcga cgaactggcg atggcccttg gccgggcagg agagggcgac cgcgccgcct
     1321 tccgccgcgt ctacgaggcg acctccgcga agctattggg ggtgaccctg cgtatcttgg
     1381 ctgaccgaca gctggccgag gacgttttgc aggacaccta tctgaccgtg gagaaaggcc
     1441 gggagcttcg atgcatcccg tgcgagcccg atcacgtggc tggtgaccat cgcccgcaac
     1501 cgcgccatcg accggctgag atcaggggcg gccacgcgcc ggtcaacgcc catcgacgac
     1561 gccttcgatg tggccgacga cggcccttcg ccggcgcagg cgctggagac cggcgacgat
     1621 gtgcgccggc tgaacgactg ccttggcgag ctggacgacc gcacaggcgg cgccatccgc
     1681 accgccttta tcgagggcgt gacctatgat gcgctcgcct cgcgtgagaa tgtgccgctg
     1741 ggaacgatga agagctggat ccggcgcggc ctgatgcggc tgagggcctg tctggaatca
     1801 tgaccgatcc gaccgacaca gaaggcatgg acgaaaacga ggccttggcc ggcgaactgg
     1861 ccttgcgcgt cctgtcgccc gaggaagagg cggcggcccg gg
//
LOCUS       DASVVN010000219         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_60119, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000219 DASVVN010000000
VERSION     DASVVN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60119"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VF686_03420"
     CDS             <1..1452
                     /locus_tag="VF686_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8660893.1"
                     /translation="DRQLDSGEYINSYILRGRRGQTLDLRLTSTAFDPYVAIDGPGDF
                     SAFNDDDPAVTDGRNSRLVVTLPADGDYRITATSYASGEQGSYRLAVLDGAGAATPPP
                     PAVRPSERASANGGITIGQTVSGSLDAGDDTLDSGEYVDNFRFTGRRGQRVAVELTSS
                     AFDAYTILRTPSGDQQENDDARDGGHDSRLDTVLAEDGEYEVMVTSYAPGETGSYRFS
                     VSPSQGSPRQASVQGGARVFAVMVGVSDYGGAQSPLDFTDEDARKLAETLRADGSLND
                     ASLVLTNAEATVAGVRRAFARVAAEAGPEDTFLFFFSGHGDQSEVAVSGLEPDGKSEA
                     IMLRDGEITDTEMGRLFGSLRTRLSLLVIDSCFSGGFARNVVDRPGVMGLFSSEEDLT
                     SAVADKFQAGGYLSHFLRTGMGGEADGDGDRLVTAGELATYLRRQFREQVENVESETQ
                     DGQRNYQNLVVDRGGVQVDDVIVRLPATAAGAQ"
     gene            complement(1466..1540)
                     /locus_tag="VF686_03425"
     tRNA            complement(1466..1540)
                     /locus_tag="VF686_03425"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1506..1508),aa:Val,seq:cac)
BASE COUNT          276 a          615 c          603 g          253 t
ORIGIN      
        1 gaccgccaac tggacagcgg cgaatacatc aacagctaca tcctgcgcgg gcggcgtgga
       61 cagacgctgg atctgcgcct gacctcgacg gccttcgacc cctatgtcgc catcgacggt
      121 ccgggcgact tctcggcctt caatgacgac gatccggcgg tgaccgacgg gcgcaacagc
      181 cgtctggtcg tcaccctgcc ggcggacggc gactaccgca tcacagccac cagctacgcc
      241 tcgggcgagc agggaagcta ccggctggcg gtgctggacg gcgccggggc cgcgacgccc
      301 cccccgcctg ccgtacgccc gtccgaacgc gcctccgcca atggcgggat caccatcggc
      361 cagaccgtca gcggctcgct ggacgcgggc gacgacaccc tcgattcggg cgaatacgtc
      421 gacaatttcc gcttcacggg ccgtcgcggc cagcgcgtgg cggtggagct gacgtcctcg
      481 gccttcgacg cctacaccat cctgaggacg ccctccggcg accagcagga gaacgacgac
      541 gcccgcgacg gcggccacga cagccggctc gacaccgtcc tggccgagga cggcgaatac
      601 gaggtcatgg tcacctccta cgctcctggc gagacgggct cataccgctt cagcgtcagc
      661 cccagccaag gctcgccgcg gcaggcctcg gtgcagggcg gggcgcgggt gttcgcggtc
      721 atggtcggcg tgtccgacta cggcggcgcc cagtccccgc tcgacttcac cgacgaggac
      781 gcccgcaagc tggccgaaac cctccgcgcc gacggttcgc tgaacgacgc cagcctcgtg
      841 ctgaccaatg ccgaggccac cgtcgcgggg gtgcgccgcg ccttcgcccg ggtggcggcc
      901 gaggcggggc cggaagacac cttcctgttc ttcttctcgg gccacggcga ccagtcggaa
      961 gtcgcggtga gcgggctgga gccggatggc aagtccgagg ccatcatgct gcgcgacggc
     1021 gagatcaccg acaccgagat gggccggctg ttcggctcgc tgcgcacccg gctgtcgctg
     1081 ctggtgatcg attcctgctt ctcgggcggc ttcgcccgta acgtggtcga ccggccgggc
     1141 gtgatggggc tgttcagctc ggaagaggac ctgaccagcg ccgtggccga caaattccag
     1201 gccgggggct atctgtccca ctttctgcgc acgggcatgg gcggcgaggc cgacggcgat
     1261 ggcgaccggc tggtcaccgc cggcgagctg gccacctatc tgcgccgcca gttccgcgag
     1321 caggtcgaga acgtcgagtc cgagacccag gacggtcagc gcaactacca gaacctggtc
     1381 gtcgaccggg gcggcgtgca ggtcgacgac gtgatcgtgc gactgccggc cacggcggcg
     1441 ggggctcaat aatcgggaag agtgatggtg gatgcgacag ggattgaacc tgtgaccccc
     1501 tcggtgtgaa cgaggtgctc tcccgctgag ctacgcatcc atcaatcggg cccgtcgtga
     1561 cggggaggcg gcgacatagc ccggtcgtcc cgggggcgca agatgtcaga tgccggcgcc
     1621 ccgcaccgcc tcgggcgtct cggcgtccgg atcgcccggg agccaggcga cgtggccgtc
     1681 atagatggcc tcgtccagaa tcccttcctc cttcgccacc agcacgggga ccagcatctg
     1741 accggtg
//
LOCUS       DASVVN010000220         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_191677,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000220 DASVVN010000000
VERSION     DASVVN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191677"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VF686_03430"
     CDS             complement(<1..831)
                     /locus_tag="VF686_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660894.1"
                     /translation="MDRRKGPPAWVRIALLTVTLAVAAYVLLFAREMARPGQEADALR
                     LSAVDREARLLALDLQTRIAPVEAAVRAAAVGTDSPSIMLQRARAAAPDAAFAVLDGI
                     GRPVATTGGDARQFAAAPGRPRLTADGRAILIARPLTGGGRLIGRAPLPSLAPQDGIN
                     RTVLSAPDTVLTGVRTSAGTLGLSAAPVAANGRGVTLRGEGRAIRRTGAPVGDTGLIA
                     VASTPITVGADALLDDVWVLTAPVGIGAFVVLLALLLQRRQQGISQVWAASEQRFRGA
                     V"
     gene            complement(968..1546)
                     /locus_tag="VF686_03435"
     CDS             complement(968..1546)
                     /locus_tag="VF686_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660895.1"
                     /translation="MARAQFQKGQKVWVECVGAWAQIEKVQPVWAKGFEEPVRVTYDV
                     GLGREFLGHELLLPTDDPAANAGETWRLMRARNKWQTADDCPHHPFPGTYPVVVTDKA
                     DWGGWRVPGAEYDRDPDQIEFQARLIAASPRLLALAERLVASVDEAPDDAPPELQELA
                     RTARTVLKTVTDVLVSPTESGGGAGQRETQAA"
BASE COUNT          234 a          609 c          611 g          250 t
ORIGIN      
        1 gacggcgccg cggaaccgct gttccgaggc cgcccagacc tggctgatcc cctgctgccg
       61 gcgttgcagc agcagggcca gcaggaccac gaacgccccg atgccgacgg gcgcggtcag
      121 cacccagacg tcgtccagca gggcgtcggc gccgaccgtg atcggcgtcg aggccacggc
      181 gatcaggccg gtgtctccca ccggggctcc ggtgcggcga atggcgcgtc cttcgccgcg
      241 cagggtgacg ccccgaccgt tggccgccac cggcgcggcc gacaggccca gggtcccggc
      301 gctcgtccgg acgccggtca ggaccgtgtc gggcgccgac aggaccgtac gatttattcc
      361 gtcctgcggc gccagcgacg gcaggggcgc acgcccgatc agtcggcctc cgccggtcag
      421 cgggcgagcg atcagtatgg ccctgccatc ggccgtcagg cgggggcggc cgggggcggc
      481 ggcgaactgg cgggcgtcgc cgcccgtggt cgccacgggg cgtccgatcc cgtccagaac
      541 ggcgaaggcc gcgtccggcg ctgcggcgcg ggcgcgttgc agcatgatcg agggagagtc
      601 cgtcccgacg gcggcggcgc gcacggcggc ctcgacgggg gcgatccggg tttgcagatc
      661 gagggccaga agacgggcct cccggtcgac ggcgctcaga cgcagggcgt ccgcctcctg
      721 tcccggtcgg gccatctcgc gggcgaacag cagcacatag gccgccacgg ccagggtgac
      781 ggtcagaagg gcgatgcgca cccacgcagg cgggcccttg cgccggtcca ctgcgcgtcg
      841 tccatctccg gttatgcggc gctcgttgcc ccgcaaggcg ctctccccga gaaatcgacg
      901 tcgatcagag gaatctaggc tgcgtcgcgg attcctgtcg agccttgctt cggaacgatc
      961 aacggtttca ggcggcctgc gtttcgcgct ggcctgcgcc gccgccgctc tcggtcggcg
     1021 aaaccagaac gtcggtgacc gtcttcagca ccgtacgcgc cgtgcgggcc agttcctgca
     1081 gctcgggcgg cgcgtcgtcc ggcgcctcat ccaccgaggc caccagccgt tcggccaatg
     1141 ccagaagccg gggcgaggcc gcgatcaggc gcgcctggaa ctcgatctgg tcggggtcgc
     1201 ggtcgtattc cgcgccgggc acgcgccagc cgccccagtc ggccttgtcg gtcacgacca
     1261 cggggtaggt gccggggaag gggtggtgcg ggcagtcgtc ggccgtctgc catttgttgc
     1321 gcgcccgcat cagacgccag gtctcgccgg cgttggcggc ggggtcgtcc gtcggcagca
     1381 gcagttcgtg gcccagaaac tcccggccca gcccgacgtc ataggtgacg cggacgggtt
     1441 cctcgaaccc cttggcccag acgggctgga ccttttcgat ctgggcccat gcgccgacgc
     1501 attcgaccca gaccttctgt cccttctgga attgcgcgcg cgccattcac gtctccgatc
     1561 ctgaaggatc agaatgcctt gcggcgggtg aacagccggt tccgcgaaag ggtgaagcag
     1621 acgtaaacgc cggccccacc ctcgtgccgc gtcaggcgcg cgagatgtct tccagcgcct
     1681 cgccggcgta tttctccttc acgc
//
LOCUS       DASVVN010000221         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_428632,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000221 DASVVN010000000
VERSION     DASVVN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428632"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VF686_03440"
     CDS             <1..903
                     /locus_tag="VF686_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8660896.1"
                     /translation="QAVKDLSFQVEKGTICGFLGPNGAGKTSTIRMILGLQGVTSGQI
                     GILGANDGRKVRDRIGFLPEERGLYKKMTPVDAIAFFGSLKGLPLAEGRRRAREMLDA
                     QGLGASKRKKMKELSKGMAQKVQLIASVVHRPEFVILDEPFSGLDPVNQQGLEAVIRG
                     LAADGATVLFSTHVMQHAERLCDKVVLLARGQKAFEGTVSEARATSKRFLELEGAIDA
                     AQAAALPGVTGVEMVSEVDGVRVLKAGLGRGGKGQEALKAAFLGGLDVRRFELREPSL
                     HDAFISLTGDNPDVDQSVKAEAAR"
     gene            900..2297
                     /locus_tag="VF686_03445"
     CDS             900..2297
                     /locus_tag="VF686_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8660897.1"
                     /translation="MNRTLLIARREYAAYAKTVGFWLSLLAFPLFAVLGGAVPILIRT
                     SEPVRAVAVIEEGPAAVGLAGAVSDALRREADRRAERAREAAEKAKAATGGAAPPPNA
                     AQGALASLGATRMRIVPTPAEITAAAPGEAQTEVVRRFLDEDTPKDKRLDAVVFLTRD
                     AEGKPGARVWTARATDDTVEDFVRDALRAANRRSVFGAAGIDGAVVEATEAFRPTVTA
                     FSPKSASGGEVSFRDRLPQIMGLAGGFLLWSLVITGASILLNSVMEEKSNKILEVLLS
                     SASATEILAGKVLGVALLTLTVLAAWGSIGAAGMIGFLPQYAGDVGDVLLNGGMLGWF
                     AAFMVGGYMMYAVLFAAIGAFCETPRDAQTLMGPIMMVLVIPMITMQMALRTPDAPVI
                     QIMSMIPFFTPFVMMARLPSEPPMIELIGSIAGMFAFALFMVWIAGRAFRAGALSDVK
                     LSWKSFAGAIRGGGT"
     gene            2294..2665
                     /locus_tag="VF686_03450"
     CDS             2294..2665
                     /locus_tag="VF686_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660898.1"
                     /translation="MKAGPRFIIVVALLIWPAACASKAATCQTSPRLVDEQAIVQLLD
                     RHIRDGQALVDGPRSIPLTELPLVQVDGVVSEGEVINDGDSAVRLIAYRSKRLPVLSY
                     LVSRRSDDACARELSWRIAAP"
     gene            complement(2727..2981)
                     /gene="rpsU"
                     /locus_tag="VF686_03455"
     CDS             complement(2727..2981)
                     /gene="rpsU"
                     /locus_tag="VF686_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX8660899.1"
                     /translation="MVQIFVRDNNVDQALKALKKKMQREGSFREMKRHVHYEKPSEKR
                     ARQKAEAVRRARKLARKRMQREGLLPPPKPRVPGVGGPRS"
     gene            3170..3844
                     /locus_tag="VF686_03460"
     CDS             3170..3844
                     /locus_tag="VF686_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX8660900.1"
                     /translation="MTEAPHLSNETSEDDWGDRMEQSVLDAAVRLAPALGWNSRMVRR
                     ACAENGLSLGDEELLLPNGARDLAALLSRRHDDRALAVLAEVDAKSLKMRERIARAVS
                     ARMEAGAADLEATRRCAAFLALPTNADLGLKLAWESADHLWHWAGDTATDWNHYSKRA
                     ILSGILIPALTMRWFDGRDAAEAFVAARIENVMAFEKWKAGKDFDAPLRKVSEALSRM
                     RYGAKG"
BASE COUNT          584 a         1249 c         1379 g          666 t
ORIGIN      
        1 caggccgtga aggacctgag ctttcaggtc gagaagggca cgatctgcgg cttcctgggc
       61 ccgaacgggg ccggcaagac ctcaaccatc cgcatgatcc tggggctgca gggcgtgacc
      121 tcgggccaga tcggcatcct cggcgccaac gacgggcgca aggtgcgcga ccggatcggc
      181 ttcctgccgg aggagcgggg cctctacaag aagatgacgc ccgtggacgc catcgccttc
      241 ttcggctccc tgaagggcct gccgctggcc gagggccgcc gccgcgcaag ggagatgctg
      301 gacgctcagg gtctcggcgc ctcaaagcgc aagaagatga aggagctgtc caaggggatg
      361 gcccagaagg tccagttgat cgcgtcggtc gtgcatcggc cggagttcgt catcctcgac
      421 gagcccttct cgggactgga tccggtcaac cagcaggggc tggaagccgt gatccgcggg
      481 ctcgcggcgg atggagcgac ggttctgttc tcgacccatg tgatgcagca cgccgaacgg
      541 ctgtgcgaca aggtcgtgct gctggcccgg ggccagaagg cgttcgaggg cacggtcagc
      601 gaggcgcgcg cgacctcgaa gcggttcctt gaactggaag gcgccatcga tgcggcccag
      661 gcggcggcct tgccgggcgt caccggtgtc gagatggtgt ccgaagtcga cggggtgcgc
      721 gtgctgaagg ccggactggg caggggcggc aagggacagg aggcgctgaa ggcggccttc
      781 ctcggcggtc tggacgtgcg gcgattcgaa ctgcgcgagc ccagcctgca tgacgccttc
      841 atcagcctga ccggggacaa tcccgacgtc gaccagtccg tgaaggcgga ggccgcccga
      901 tgaaccgcac gctcctgatc gcccggcgcg aatacgccgc ctacgccaag accgtgggct
      961 tctggctgtc gctgctggcc ttcccgctgt tcgccgtgct cggcggcgcg gtgccgatcc
     1021 tgatccgcac gtccgaacct gtccgcgccg tggcggtgat cgaggaaggt cccgccgccg
     1081 tcgggctggc cggcgccgtc agcgacgcgc tacgccgcga ggccgaccga cgggccgaac
     1141 gcgcgcggga agcagcggag aaggcgaagg ccgccaccgg cggggccgcc ccgccgccga
     1201 atgccgccca aggcgccctg gcgtcgctgg gcgcgacccg gatgcgcatc gtcccgacgc
     1261 cggccgagat cacggccgcc gcgcccggcg aggcccagac cgaggtcgtc cggcggtttc
     1321 tcgatgagga tacgcccaag gacaaacggc tcgacgccgt ggtcttcctg acccgggacg
     1381 ccgagggcaa gccgggcgct cgcgtctgga ccgcccgggc caccgacgac acggtcgagg
     1441 atttcgtgcg cgacgcgctt cgggcggcga accgtcgctc ggtgttcggc gcggccggta
     1501 tcgacggcgc ggtggtcgag gcgaccgagg ccttccgtcc gacggtgacg gccttctcgc
     1561 caaaatccgc cagcggaggc gaggtctcgt ttcgggatcg tctgccccag atcatgggtc
     1621 tggcgggcgg cttcctgctc tggtcgctgg tcatcactgg cgcctcgatc ctgctgaaca
     1681 gcgtgatgga agagaagtcg aacaagatcc tcgaggtgct gctctcctcc gcctcggcga
     1741 ccgaaatcct cgccggcaag gttctggggg tggccttgct gaccctgacc gttctggccg
     1801 cctggggctc gatcggcgcg gcgggcatga tcggcttcct gccgcagtat gcgggcgatg
     1861 tcggcgatgt gctgctgaat ggcggcatgc tgggctggtt cgcggccttc atggtcggcg
     1921 gctatatgat gtacgcggtc ctgttcgccg ccatcggcgc cttctgcgaa acgccgcgcg
     1981 acgcccagac cctgatgggg ccgatcatga tggtgctggt catccccatg atcaccatgc
     2041 agatggcgct caggaccccg gatgcgccgg tcatccagat catgtcgatg atccccttct
     2101 tcaccccctt cgtcatgatg gcgcgcctgc cgtccgagcc gccaatgatc gagctgatcg
     2161 gctctatcgc cggcatgttc gccttcgccc tgttcatggt gtggatcgcc ggacgggcgt
     2221 tccgcgccgg ggcgctgtcc gacgtgaagc tgagctggaa gagcttcgct ggtgcgatca
     2281 ggggcggggg gacgtgaagg ctgggccgcg cttcataatt gttgtcgcac tgctgatctg
     2341 gccggcggca tgcgcctcaa aggcggcaac ttgtcagacg agccctcgtc tcgtcgatga
     2401 gcaagccatc gtgcagttgc tcgatcggca tattcgggac ggtcaggcgc tcgtcgatgg
     2461 gccgcggtcc ataccattga cggagttgcc tctggttcag gtcgacggcg tggtctctga
     2521 gggtgaggtg atcaatgacg gagacagtgc ggtgcgcttg atcgcctacc ggtccaagcg
     2581 cttaccggtg ctttcgtatc tcgtgtctcg ccgttctgac gatgcctgcg cgcgagagct
     2641 ttcctggcgc atcgccgcgc cgtgacaaga aaagggccgc tccggtttcc tggggcggcc
     2701 cttgatcgtt cagacaggtc gttcgctcag gagcgcggac cgcccacgcc cggcacgcgg
     2761 ggcttcggcg gcggcagcag gccttcgcgc tgcatgcgct tgcgggccag tttgcgggcg
     2821 cggcgcacgg cctcggcctt ctggcgggcg cgcttctccg agggcttctc gtagtgcacg
     2881 tggcgcttca tctcgcggaa gctgccttcg cgctgcatct tcttcttgag ggccttcagc
     2941 gcttgatcga cgttattatc gcgaacgaaa atctgtacca ggttgaactc tcctttggcc
     3001 gcccgccgcg cacctcgaaa gggagacggg cagacaaata aaatcgtctt ccggggacag
     3061 ggtccgcgga tgaaggcggg ctgatacacg tttgcccccc cgctgtccag atcgtcgcgc
     3121 ttcaatctga agggtcgaaa cgcccgcgcg acgggcatat gctgttgcca tgaccgaagc
     3181 gccacacctt tcgaacgaga cgtctgagga cgattggggc gaccggatgg agcagtccgt
     3241 gctggacgcc gccgtgcggc tggctcccgc gctgggctgg aacagccgga tggtgcggcg
     3301 ggcctgcgcg gagaacggcc tgtcgctggg tgacgaggag ctgctgctgc ccaatggcgc
     3361 gcgggatctg gcggccctgc tgtcgcgccg ccacgacgac cgggcgctgg ccgtgctggc
     3421 cgaggtcgat gcgaagtcgc tgaagatgcg cgagcgaatc gcccgcgccg tctcggcccg
     3481 gatggaggca ggggccgccg atctggaggc cacgcgccgc tgcgccgcct ttctggccct
     3541 gccgaccaac gccgatctgg gtctgaagct ggcgtgggag agcgcggatc acctgtggca
     3601 ttgggccggg gacacggcga ccgactggaa ccactattcc aagcgggcca tcctgtcggg
     3661 catcctgatc cccgccctga ccatgcgctg gttcgacggc cgggacgccg ccgaggcctt
     3721 cgtggccgcc cgtatcgaga acgtcatggc cttcgagaaa tggaaggcgg gcaaggattt
     3781 cgacgcgccg ctgcgcaagg tcagcgaggc gctgagccgg atgcggtatg gggcgaaggg
     3841 ctaggccttc ctgtctcctc cctgttgcga agcaatgg
//
LOCUS       DASVVN010000222         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_7526, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000222 DASVVN010000000
VERSION     DASVVN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7526"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1707)
                     /locus_tag="VF686_03465"
     CDS             complement(28..1707)
                     /locus_tag="VF686_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8660901.1"
                     /translation="MSRGGDLSERALVLAPNGRDAAIAAAILKEAGVAAQVCETLESL
                     RLRLDEGAALAVVVEEALRDCDLTGLSAWIEAQPSWSDFPFVVLSQRGGSVERNPVAR
                     RLSVTLGNVSFLERPFHPTTLVSAVHTALRGRKRQYEARERIEEIRRAEALLERRVAE
                     RTAELEAANRQLASQIGEREKAESALRQAQRLEAVGQLTSGVAHDFNNLLTVVMGNVE
                     QLRKQETDPKVLRRLDMMSEASRRGARLTAQMLAFSRRQKLEPRAVDLNETVTSMGDM
                     LQSAIGGSVHIEPPQLATDLWPAMIDPTQIELVILNLAINARDAMAVGGSLTIRTENC
                     TVGDPRRPEEPPAGDYVMVSVADTGSGMTEEVLSRAFEPFFTTKEVGKGSGLGLSQVF
                     GLAKQSGGGVSIDTELGRGSTIRIFLPRAGSAPVRHGEPSGELETARDPGLVILVVDD
                     DDAVREVTAGFLTDLGYEVVEAGSGGAALEQLDQHERIDAMVLDFAMPGMNGGDLARE
                     LRMRRPSAPILFATGSVDVEALTDVGEDMIVHKPFTQAELARKLSRSLAGV"
     gene            complement(1704..3200)
                     /locus_tag="VF686_03470"
     CDS             complement(1704..3200)
                     /locus_tag="VF686_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX8660902.1"
                     /translation="MTDGVEREKQARFGVAGLDDILSGGLARDRLYLLEGNPGTGKTT
                     AAMSFLMAGADHGESCLYITLSETEEELRDTARSHEWELEGIEIFELVPPESLLDEQQ
                     QQSLLYSSDLELGETTRMIIEAVEQASPDRVVIDSLSEIRLLAQGSLRYRRQVLALKH
                     YFAKRGTTVLLLDDLTTDTNDKTVHSVAHGVIQLQELAPEYGAERRRVRVIKYRGRRF
                     RGGYHDFTIKTGGLEVYPRIVASEHRTAFDRTKVSSGVPGLDELLGGGVERGSSALIL
                     GPAGTGKSLFAVNFAISAVKRGEKAALFIFDEELGLLFDRMRGLGIDLEAMQADGSLM
                     IEQVDAAELSPGEFASRVRKCVSEKQIKTVIVDSLNGYQAAMPQEQFLVLHIHELLQY
                     LNRQGASTFLTVAQHGLVGDMKAPVDVTYLADTVILLRYFEATGHVRRAVSVIKKRAG
                     GHEKTIREFDIGADGLSMGEPLHGFQGVLRGVPNFVGQGSGLMGEASA"
     gene            complement(3277..>4386)
                     /locus_tag="VF686_03475"
     CDS             complement(3277..>4386)
                     /locus_tag="VF686_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX8660903.1"
                     /translation="KGGRIALVGPTIRDARTVMVEGPSGVLNLPFRMGATFASSLRQV
                     TFPGGAVGLLFSAEEPERLRGPQFHAAWGDEFCAWGRCEETLAMLRLGLRLGEGPRLV
                     LTSTPKPGRVLRGVLCEDGCARTDAPTRVNAKNLAPGFLAAMEGLYGGTRRAAQELEG
                     RVLESDGALFTLAEIERARAGWSEGEGYERGSSSATRGSPFERVVVAVDPPAGTLSGR
                     ARDACGIVVAGRRGDRVGVLADWTARGLSPEGWADRVRRAVEAYGAGKVVVESNNGGA
                     MAQAVLKASGLTVAVKRVHASVGKVARAEPVAALYEQGRVAHAPGLEALEEELMGLGS
                     EEAGGVSPDRADALVWAVTELAPAPGTRGPRMTQL"
BASE COUNT          652 a         1819 c         1249 g          666 t
ORIGIN      
        1 ggccctcgac cctcgggagg tcgccggtca gaccccggcc agggagcgcg acagtttgcg
       61 ggccagctcg gcctgggtga acggcttgtg cacgatcatg tcttcgccga cgtcggtcag
      121 cgcctcgacg tccacagagc cggtggcgaa caggatcggc gccgaggggc ggcgcatcct
      181 gagttcgcgc gcgaggtcgc cgccgttcat cccgggcatg gcgaaatcca gcaccatggc
      241 gtcaatccgt tcgtgctggt ccagctgctc cagggccgcg ccgccgctgc ccgcctcgac
      301 gacctcatag ccgaggtcgg tcaggaagcc ggcggtcacc tcccgcaccg cgtcgtcgtc
      361 gtcgacgacc agaatgacca gtcccggatc ccgcgccgtc tccagttcgc cgctcggctc
      421 gccatgacgc acgggcgccg agccggcccg cggcagaaag atgcggatcg tcgagccgcg
      481 gcccagttcg gtgtcgatgc tgacgccgcc gcccgactgt ttggccaggc cgaagacctg
      541 gctcaggccg agaccggatc ccttgccgac ctccttggtg gtgaagaagg gctcgaaggc
      601 ccggctcagc acctcctcgg tcatccccga tccggtgtcg gcgaccgaca ccatcacata
      661 gtcgccggcc ggcggctcct ccggccggcg cgggtcgccg accgtgcagt tctccgtccg
      721 gatcgtcagc gagccgccca cggccatggc gtcgcgggca ttgatggcca ggttcaggat
      781 caccagctcg atctgggtcg ggtcgatcat cgccggccag agatcggtgg ccagttgcgg
      841 cggctcgata tggacgctgc cgccgatcgc gctctgcagc atgtcgccca tgctggtgac
      901 cgtctcgttc aggtcgaccg cccgcggctc cagcttctgc cggcgcgaga aggccagcat
      961 ctgggcggtc agccgcgccc cgcgccgcga ggcctcgctc atcatgtcca gacggcgcag
     1021 caccttcgga tcggtctcct gcttcctcag ctgttcgaca ttgcccatga cgacggtcag
     1081 caggttgttg aagtcgtgcg ccacgccgct ggtcagctgt ccgaccgcct ccagccgctg
     1141 cgcctgccgc agggcggatt cggccttctc gcgctcgccg atctgcgagg cgagctgccg
     1201 gttggcggcc tccagttcgg cggtgcgttc cgccacccgc cgctccagca gcgcctcggc
     1261 gcgccggatc tcctcgatcc gctcccgcgc ctcatactgc cgcttgcgtc cccgcaacgc
     1321 cgtatggacg gcgctgacca gggtggtcgg atggaacggg cgttccagaa aactgacatt
     1381 gcccagcgtc accgacagcc ggcgcgccac ggggttccgc tctacgctgc cgccccgctg
     1441 cgacagcacc acaaacggaa agtccgacca cgacggctgc gcttcgatcc acgccgaaag
     1501 gccagtcaga tcgcagtccc tcagagcctc ctcgaccacc accgccagag ccgccccctc
     1561 gtcgagccgc aggcgcaggc tctccagcgt ttcgcacacc tgcgccgcca cccccgcctc
     1621 cttcaggatg gcggccgcga tggcggcgtc gcgcccgttc ggagccagca ccagcgcccg
     1681 ttcggacagg tcgccccccc ggctcatgcc gaggcttcgc ccatcagtcc cgagccctgt
     1741 ccgacaaaat tcggcacgcc gcgcagcacg ccctggaaac cgtgcagcgg ctcgcccatc
     1801 gacagcccgt ccgcgccgat atcgaactcg cggatcgtct tctcatggcc gccggcgcgc
     1861 tttttgatca ccgagaccgc ccgccgcaca tggcccgtcg cctcgaaata gcgcagcagg
     1921 atcaccgtat cggccagata ggtcacgtcg accggcgcct tcatgtcccc gaccagccca
     1981 tgctgggcca cggtcaggaa ggtcgaggcg ccctgccggt tcagatactg cagcagctcg
     2041 tggatgtgca gcaccaggaa ctgctcctgc ggcatcgccg cctgatagcc gttcagactg
     2101 tccacgatga cggtcttgat ctgcttctcc gacacgcatt tgcgcacccg tgaagcgaac
     2161 tcgcccggcg acagctcggc cgcgtcgacc tgttcgatca tcagcgaccc gtccgcctgc
     2221 atggcctcca gatcgatgcc cagaccgcgc atgcggtcga acagcagccc cagctcctcg
     2281 tcgaagatga acagcgccgc cttctcgccg cgcttgaccg ccgatatcgc gaaattgacc
     2341 gcgaacagcg acttgcccgt gcccgccggc cccaggatca gggcgctcga gccgcgctcc
     2401 accccgccgc ccagcagctc gtccagcccc ggcaccccgc tggacacctt ggtccggtcg
     2461 aaggccgtgc ggtgctccga ggccacgata cgcggataca cctccagccc gcccgtcttg
     2521 atggtgaagt cgtgatagcc gccgcggaac cgccgcccgc ggtatttgat cacccgcacc
     2581 cgccgccgct cggcgccata ctcgggcgcc agctcctgca gctggatcac cccgtgcgcc
     2641 acagaatgaa ccgtcttgtc gttggtgtcc gtggtcaggt cgtccagcag cagcaccgtc
     2701 gtcccgcgct tggcgaaata gtgtttcagc gccagcacct gccgccggta gcgcagcgag
     2761 ccctgggcca gcagccggat ctcggaaaga ctgtcgatca ccacccggtc cggcgaggcc
     2821 tgctccaccg cctcgatgat catccgcgtc gtctcgccca gctccaggtc cgacgaatac
     2881 agcaggctct gctgctgctg ttcgtccagc aggctctcgg gcggcaccag ttcgaagatc
     2941 tcgatcccct ccagctccca ttcgtgcgac cgcgccgtgt cgcgcagctc ctcctcggtc
     3001 tcggacaggg tgatgtacag gcacgactcg ccgtgatccg cccccgccat caggaagctc
     3061 atcgccgccg tcgtcttgcc ggtgccgggg ttgccctcca gcagatacag ccggtcgcgc
     3121 gccagtccgc cggacagaat gtcgtccagc ccggccacgc cgaaccgcgc ctgtttttcc
     3181 cgttccacgc cgtccgtcat acgccctctc tgctttcagc tgagccataa cgcacggaca
     3241 tccataaggg ttcagcccca cggccacgct tcccgcctat agctgggtca tcctcggccc
     3301 ccgcgtcccc ggcgccggcg ccagctccgt caccgcccag accagcgcat ccgcccggtc
     3361 cggactgacc ccccccgcct cctcgctgcc cagccccatc agctcctcct ccagcgcctc
     3421 cagccccggc gcatgagcca cccgcccctg ctcgtacagc gccgccaccg gctcggcccg
     3481 cgcgaccttg ccgaccgacg catgcacccg cttcaccgcc accgtcagcc ccgaggcctt
     3541 cagcaccgcc tgcgccatcg ccccgccgtt gttgctttcg accaccacct tgcccgcccc
     3601 gtatgcctcc accgcccgcc gcacgcgatc cgcccagccc tccggggaca acccccgcgc
     3661 cgtccagtcc gccagcaccc cgacccggtc cccgcgccgc cccgccacca cgatcccgca
     3721 cgcatcccgc gcccgccccg acagcgtccc cgccggcgga tcgaccgcca ccaccactct
     3781 ttcgaagggg ctaccgcgcg tcgcgctact tgagccccgc tcatacccct cgccctcgct
     3841 ccaccccgcc cgcgcccgct cgatctccgc cagggtgaac agcgccccgt cgctctccag
     3901 cacccgccct tccagctcct gcgccgcccg ccgcgtcccg ccgtacagcc cctccatcgc
     3961 cgccagaaac cccggcgcga ggttcttcgc attcacccgc gtcggtgcat ccgtccgcgc
     4021 gcacccgtcc tcgcacagca ccccgcgcaa cacccgcccc ggcttcggcg tcgacgtcag
     4081 aaccagccgc ggcccctccc ccaaccgcaa ccccagccgc agcatcgcca gcgtctcttc
     4141 acaccgcccc cacgcacaaa actcatcccc ccacgccgca tggaactgcg gcccgcgcag
     4201 ccgctccggc tcctcggccg agaacagcag ccccacggcc ccgcccggaa acgtcacctg
     4261 tctcagcgac gaggcaaacg tcgcccccat ccggaacggc aggttcagca cccccgacgg
     4321 cccctcgacc atcaccgtcc gcgcatccct tatcgtaggc ccgaccagcg caatccgccc
     4381 gccctt
//
LOCUS       DASVVN010000223         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_507390,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000223 DASVVN010000000
VERSION     DASVVN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507390"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..793)
                     /gene="phnE"
                     /locus_tag="VF686_03480"
     CDS             complement(35..793)
                     /gene="phnE"
                     /locus_tag="VF686_03480"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX8660904.1"
                     /translation="MGAWALDILIWGGVAALLIYSFGPVELANVTKLFSNNEHTQNFA
                     RELLRPDFENWKLFVAKMWETVQIALWGTFIAVFAAVPMGLAAARNIAPAWVVTPVRW
                     LMNLLRSIPDLVIGLLFVVAVGLGPLAGVLAIALNTAGVLAKLFSEAVESIDKGPVEG
                     VRATGASKLHEIVWGVIPQVAPLWTSFALYRFESNSRSATVLGLIGAGGIGQVLFDSM
                     NAFDFRDVSAIVVVVVVAVTLIDMLSQAMRKRLL"
     gene            complement(850..1851)
                     /gene="phnD"
                     /locus_tag="VF686_03485"
     CDS             complement(850..1851)
                     /gene="phnD"
                     /locus_tag="VF686_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8660905.1"
                     /translation="MTFAPVTRRLGLIAAAVAMLGLASCGAEGEKSSAAPTELTFSIL
                     SAEGQASAGPLWQPLLDDMSKAVGVPVKPYFGSNYTVLVEAMRGNQTQVAWFSAKPAV
                     EAIDRADAEVIARTVNKEGLDSYRSTLIVRTGSGITLDQVMACGKRFDFGIGDAQSTS
                     GTLAPMAFLFNPRGVVPAQCFKTVRSANHQSNAFSVASGVLDVATSNTVNTVFLRKQN
                     PQIAAQIQEIWQSPPIPESGILVREDLDPALKEKIRSFFLTYGQGEGVEAERQRQVLA
                     GLEYSRFNAADDGYLNPIREMVADQKLGEARAKGDAAGVAAAERELQRLRSLREVQP"
     gene            complement(1848..2645)
                     /gene="phnC"
                     /locus_tag="VF686_03490"
     CDS             complement(1848..2645)
                     /gene="phnC"
                     /locus_tag="VF686_03490"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX8660906.1"
                     /translation="MTSATAAVASVKDVSKTFGARKALNGVSVEVGAGEMVALIGPSG
                     SGKSTLLRSITGLQTIDSGKGTITVFGECVQKNGRTTGATRAARQKLGMIFQQFNLVG
                     RLSLFSNVMLGALGRLPGWQGFLGAWPGADKQRAMAALHRVGVSDYAAQRANTLSGGQ
                     QQRGAIARALVQGAKAILADEPVASLDPVSARKVMELLVELNKRDGLGVIVTLHQVDY
                     AIRYCDRVIALKAGQIVYDGPATGLDTKQLIDIYGPEFEDAFWETKA"
BASE COUNT          491 a          915 c         1024 g          456 t
ORIGIN      
        1 ggtggcgggc ttttcattgg gcgatgtctc aggcctacag cagtcgcttg cgcatggcct
       61 gggacagcat gtcgatcagg gtcacggcga ccacgaccac gaccacgatg gccgagacgt
      121 cgcggaagtc gaaggcgttc atgctgtcga acaggacctg accgatgccg ccggcgccga
      181 tcagacccag cacggtagcc gagcggctgt tcgattcgaa ccggtagagg gcgaacgagg
      241 tccacagcgg cgcgacctgc gggatcacgc cccagacgat ctcgtgcagc ttggaggcgc
      301 cggtggcgcg cacgccctcg accggcccct tgtcgatcga ctcgacggct tccgaaaaca
      361 gcttggccag cacgcccgcg gtgttcaggg cgatggcgag aacgcccgcc agcggcccga
      421 ggcctacggc gacgacgaac agcaggccga tgaccagatc ggggatcgag cgcagcaggt
      481 tcatcagcca gcgcaccggc gtgacgaccc aggcgggggc gatgttgcgc gccgcggcca
      541 gacccatggg aacggcggcg aagacggcga tgaaggtgcc ccacagggcg atctgcaccg
      601 tctcccacat cttggcgacg aacagcttcc agttttcgaa atcggggcgc agcagttcgc
      661 gggcgaaatt ctgggtatgt tcgttgttgc tgaacagctt ggtgacattg gccagctcga
      721 ccgggccgaa gctgtagatc agcagggcgg cgaccccgcc ccagatcagg atgtcgagcg
      781 cccaggcgcc catcgatttc gtgggcgggt tggggatggc ggtcgcggcg ggccgggcgg
      841 cggtatcggt caaggctgga cctcgcgcag gctgcgcagg cgctgcagtt cacgctcggc
      901 ggcggcgacg ccggcggcgt cccccttggc gcgggcttcg cccagcttct ggtcggcgac
      961 catttcccgg atcgggttca gatagccgtc atcggcggcg ttgaagcgcg agtattccag
     1021 ccccgccagc acctgacgct ggcgttcggc ctcgacgccc tcgccctggc cgtaggtcag
     1081 gaagaagctg cggatcttct ctttcagcgc gggatcgagg tcctcgcgca ccaggatgcc
     1141 gctctccggg atcgggggcg attgccagat ttcctgaatt tgggcggcga tctgcgggtt
     1201 ctgcttgcgc aggaagacgg tgttgacggt gttcgacgtc gccacgtcca gcacgcccga
     1261 ggccaccgag aaggcgttcg actggtgatt ggccgagcgg acggtcttga agcactgcgc
     1321 cggcacgacg ccccgcggat tgaacaggaa ggccatcggg gccaacgtgc cggaggttga
     1381 ctgggcgtcg ccgatgccga agtcgaaccg cttgccgcag gccatgacct gatccagcgt
     1441 gatgcccgag ccggtgcgga cgatcagggt cgagcggtag ctgtccagcc cttccttgtt
     1501 gacggtacgg gcgatgacct cggcgtcggc gcggtcgata gcctcgacgg cgggcttggc
     1561 cgagaaccag gcgacctggg tctggttgcc gcgcatggcc tcgaccagga cggtgtagtt
     1621 cgagccgaaa tagggtttta ccggcacgcc gacggccttg gacatgtcgt ccagcagcgg
     1681 ctgccacaac gggccggcgg aagcctgacc ctcagcggac aggatggaga aggtcagctc
     1741 ggtcggtgcg gccgaggact tctcgccttc ggcgccgcac gaagcgaggc cgagcatggc
     1801 gacggcggcg gcgatgaggc cgagccggcg ggtgacgggt gcgaaggtca tgccttggtt
     1861 tcccagaacg cgtcctcgaa ctcgggaccg tagatgtcga taagctgttt ggtgtccagg
     1921 ccggtggcgg gcccgtcata gacgatctgg ccggccttca gggcgatgac ccggtcgcaa
     1981 tagcggatgg cgtagtcgac ctggtgcagg gtgacgatga cgcccagacc gtcgcgcttg
     2041 ttcagttcga ccagcagttc catcaccttg cgggcggaca cgggatccag cgaggcgacg
     2101 ggctcgtcgg cgaggatggc tttcgcgccc tgaacgagcg cccgggcgat ggcgccgcgc
     2161 tgctgctggc cgccggacag ggtgttggcg cgctgggccg cataatcgga cacgccgacg
     2221 cggtgcaggg ccgccatggc gcgctgtttg tccgcgccgg gccaggcgcc gaggaagccc
     2281 tgccagccgg gcagacgtcc gagcgcgccc agcatgacgt tggaaaacag cgacaggcgg
     2341 ccgaccagat tgaactgctg gaagatcatg cccagcttct ggcgcgcggc gcgggttgcg
     2401 ccggtcgtac ggccgttctt ctggacgcat tcgccgaaga cggtgatcgt tcccttgccg
     2461 gaatcgatgg tctgcagacc ggtgatcgaa cgcagcagcg ttgatttccc cgacccggac
     2521 ggaccgatca gggcgaccat ttctccggcc ccgacctcga cggacacgcc gttcagggcc
     2581 tttcgggcgc cgaaagtctt ggacacgtcc ttcacggacg cgacggcggc agtggctgag
     2641 gtcatggaca cgctgaaagc ggttgcaagg gcgcgccgca tggcccgatc aggcccgcga
     2701 ggcaagtctt aatgacacgt ccgcgaggat ttcgtccagc ggaacagcag gcgccgcacg
     2761 ggacgccccg gccgccgccg caagggccga gcccttcgcc gccggatggt ccgcaggcgg
     2821 acaaatcccg cgcaagtcca agccccgcct agaaaaaacc gctttacatg acaggtccat
     2881 gaccct
//
LOCUS       DASVVN010000224         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_480757,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000224 DASVVN010000000
VERSION     DASVVN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480757"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF686_03495"
     CDS             <1..445
                     /locus_tag="VF686_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660907.1"
                     /translation="VARTLTEVCLPYATRAQSFEKSIRAARDLGYRRPIGSNAPLDDW
                     ASEIDLISTDGNWRIRIEEGTIEEGDSSVYAASCTMSSRHASARELAELGRRAFSDPL
                     RWSFSPDNPRRWERRTPRPEERRLSVEVAEPAEQLPTMTITGSYF"
     gene            complement(436..867)
                     /locus_tag="VF686_03500"
     CDS             complement(436..867)
                     /locus_tag="VF686_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX8660908.1"
                     /translation="MTPHLSIDDTLAELVEEFDLLGDWEGRIEYVIELGKGLAALPDE
                     DRIEPNKVPGCAAQVWLAVRRHEDRLTFLADSDSALSKGNIALLLKLYSDRRPEEILA
                     FDARAALDRLGLPSALTRQRANGLNAMVGRIRDAAASAAQK"
     gene            1040..>1942
                     /locus_tag="VF686_03505"
     CDS             1040..>1942
                     /locus_tag="VF686_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8660909.1"
                     /translation="MIQSARPDPRPAIDPDRGDRPALAAWHAGWAVAAALLALGGRWI
                     GGVDGPVFYGVLAMVVPGLTGLGLLRRDGADERMMLLGVWGLAAFLSAALSGGLTGPL
                     TGFVFLPLAAGIALGGTRPVQAGALAVGFAALAGLLSAWLAGPGPHLPALAAISALLT
                     AGAAALAVRLSWRQRERRLETAEADAARIETLLASQPGLMLILEPSGRVVAAYGAPPA
                     YLPVDPLFESGLVAAVHAPDRPRVLGAMERAMGGQDGSALFAPRTALDRRAQMIVRRL
                     DDAAGSRLVAQIFDGTAQYARELGL"
BASE COUNT          253 a          727 c          663 g          299 t
ORIGIN      
        1 ggtggcgcgc acgctcaccg aggtttgcct gccctacgcc acccgggccc agagcttcga
       61 aaaatcgatc cgcgccgcgc gggatctcgg ataccgccgg cccatcggga gcaatgcgcc
      121 cctggacgac tgggcgtcgg agatcgatct gatctccact gacggaaact ggcgcatccg
      181 catcgaggag ggcaccatcg aagaaggcga tagttccgtc tacgccgcca gctgcaccat
      241 gtcttcccgg cacgccagcg cgcgcgagct ggccgaactg ggccgccgcg ccttcagcga
      301 ccccctgcgc tggtccttct cgccggacaa tccccgacgc tgggaacgcc gcacgccccg
      361 gccagaggag cgccgccttt ccgtcgaggt tgcggagcct gcggaacagc tcccgaccat
      421 gacgataacc ggctcctact tctgagccgc cgacgccgcg gcgtcgcgga tccgccccac
      481 catggcgttc aggccattgg cgcgctggcg cgtcagggcg gacggcagcc ccagacgatc
      541 gagcgccgcg cgagcgtcga acgccaggat ttcctccggc cgacgatctg aatacagctt
      601 cagcagcagg gcgatattgc ccttcgacag ggcgctgtcg ctgtcggcga ggaaggtcag
      661 gcggtcttca tgccgccgca ccgccagcca gacctgggcg gcgcagcccg gcaccttgtt
      721 gggttcgatc cgatcctcgt ccggcaaggc cgccagaccc ttgcccagtt cgatgacata
      781 ttcgatccgg ccctcccagt cgcccaggag atcgaactct tccaccagct cggccaaggt
      841 gtcgtcgatt gagagatgcg gcgtcatggg cggcaaatag gccgggtccg cgttaacgac
      901 aaccttgcga cctgtccccg gaaaagcggc ccgaccgggc ggcgctagtc gcctggaacg
      961 cgtgcgcgct ttggacgact catccgcgag gctaggctgg acggccgccg gagtccccgg
     1021 cctcctcctg tcgcaccctt tgatccagtc cgcccgccca gaccccaggc ccgctatcga
     1081 ccccgaccgg ggcgatcgcc cggcgctggc cgcctggcac gccggctggg ccgtggccgc
     1141 cgccctgctc gcgctgggcg gacgatggat tggcggcgtg gacgggccgg tcttctacgg
     1201 cgtcctcgcc atggtggtgc ccggcctgac gggtctgggc ctgctgcgcc gggacggcgc
     1261 ggacgagcgg atgatgctgc tgggcgtctg gggtctggcc gccttcctgt ccgccgcgct
     1321 ttcgggcggc ctgaccggac ccttgaccgg cttcgtcttc ctgccgctgg cggcgggcat
     1381 cgccttgggc ggaacccggc cggtccaggc cggggcgttg gccgtcggct tcgcagccct
     1441 ggccggtctg ctgtcggcct ggctggccgg accgggtccg catctgcccg cgctggccgc
     1501 gatctccgca ctgctgaccg cgggcgccgc ggccctggcc gtacgcctgt cgtggcggca
     1561 gcgcgaacga cggctggaaa ccgccgaagc ggacgccgcc cggatcgaaa ccctgctggc
     1621 ttcccagccc ggcctgatgc tgatcctcga gccttccggc cgggtggtgg ccgcctatgg
     1681 cgcgccgccc gcctacctgc ccgtcgaccc cctgttcgaa agcggcctcg tcgccgcggt
     1741 tcacgccccc gaccggcccc gcgttctggg cgccatggag cgggccatgg gcggtcagga
     1801 cggctcggcc ctgttcgcgc cgcgcacggc gctggaccgc cgggcgcaga tgatcgtccg
     1861 ccggctcgac gacgccgcgg gttcgcggct ggtcgcccag atcttcgacg gcaccgccca
     1921 gtacgcccgc gagctggggt tg
//
LOCUS       DASVVN010000225         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_297395,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000225 DASVVN010000000
VERSION     DASVVN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297395"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="flgC"
                     /locus_tag="VF686_03510"
     CDS             complement(<1..350)
                     /gene="flgC"
                     /locus_tag="VF686_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX8660910.1"
                     /translation="MPDPIPPRNSAMAVAASALKAQQSRMRVIAENIANAQSTAQVAG
                     GQPYRRQVPVFQAREIDGATGVTLAEVRPDMSDFRSEYDPGHPAANAEGYVLRPNVDT
                     LVEAMDMREAQRAY"
     gene            complement(362..769)
                     /gene="flgB"
                     /locus_tag="VF686_03515"
     CDS             complement(362..769)
                     /gene="flgB"
                     /locus_tag="VF686_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX8660911.1"
                     /translation="MGITDLPLLGQIKGRLSWLDERQRVIAQNVANADTPGYVSRDLN
                     QPADFAAAMREGGGGVRMTRTSAMHIAPVGQVARFQSQAAPDSETTLDGNSVVVEEQM
                     LKMAESRMAYDAAIGFYQKSMQMLRMAARAPGR"
     gene            887..1174
                     /locus_tag="VF686_03520"
     CDS             887..1174
                     /locus_tag="VF686_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX8660912.1"
                     /translation="MNLLDLLRAVFALAFTLGIIGLAAWAARRYAPSLLARLSAERGE
                     KRMKVVETLMLDPARRLVLVSIDGEERLLMLGEGRELIEPRGAVTPSGAAQ"
     gene            1195..1995
                     /gene="fliP"
                     /locus_tag="VF686_03525"
     CDS             1195..1995
                     /gene="fliP"
                     /locus_tag="VF686_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170476.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX8660913.1"
                     /translation="MPTRAELKRAAILSLITTLACLLWPLGALAQDIAAAGGPGAAIN
                     VDLGTGAGLTERVVQLVGLMTVLSLAPSIVIMTTSFVRIVVVLSLLRTALGMQQSPPN
                     AVLVSLALFLSAIVMAPTWQDAYDSGIRPLLDQQMELPQAFDAASEPVKTFMLAQVDR
                     DDLALFVRLSQLEAPTATADLPLRVVTPAFMISELKKAFEIGFLLFVPFLVIDLVVAS
                     VLMSMGMMMLPPVVISLPFKLIFFVLVDGWRLVAGSLVESFQRASGTG"
     gene            2114..2392
                     /locus_tag="VF686_03530"
     CDS             2114..2392
                     /locus_tag="VF686_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX8660914.1"
                     /translation="MTTQAELIASVAKDAGVSQADAGRMLDALVKNIHKSLTGGGDVR
                     ISNLGVFDTAARAAREGRNPATGATIKIAASKAVRFRVSKPLKDAVNK"
     gene            complement(2404..>3143)
                     /locus_tag="VF686_03535"
     CDS             complement(2404..>3143)
                     /locus_tag="VF686_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660915.1"
                     /translation="MVRRLARRLVDVDLLDQAAELLKYQVENRLEGVAKAQVATDLAT
                     VYLMDRQPEPALQAIWGSRSTLLPGPLNAERRALEARALTQLGRYDHALEILGDDASP
                     EAREVRAEIFWKQQKWPQAAALYEARLGQRFADPAPLTAEEEARLIRAGVGYSLGHDA
                     AGLNRLSRNYRPFIAGARAKTALSIALDDDGVAPGDFAGLTASADTFAGWVTAMKAEL
                     RTKTGGNRAAAPARPQAAAAPPARPAA"
BASE COUNT          461 a         1071 c         1068 g          543 t
ORIGIN      
        1 tcgtaggcgc gctgggcctc gcgcatgtcc atcgcctcga ccagggtgtc gacgttggga
       61 cgcaggacat agccctcggc gttggcggcc gggtgacccg gatcatactc ggaacgaaag
      121 tcgctcatgt cgggccggac ctcggccagg gtcaccccgg tggcgccgtc gatctcgcgc
      181 gcctggaaga cggggacctg acggcggtag ggctggccgc ccgccacctg cgccgtggat
      241 tgtgcgttgg cgatattctc ggcgatgacc cgcatgcgcg actgctgggc cttcagggcg
      301 ctggccgcga cggccatggc gctgttccgc ggggggatgg ggtcgggcat cggtcagctc
      361 ctcatcggcc gggcgcgcgg gcggccatgc gcagcatctg catggacttc tggtagaagc
      421 cgatcgcggc gtcataggcc atgcggctct cggccatctt cagcatctgc tcctcgacca
      481 cgaccgagtt gccgtcgagc gtggtctcgg aatcaggcgc agcctgactc tggaagcggg
      541 cgacctgccc gaccggcgcg atatgcatgg cgctggtgcg ggtcatgcgg accccgcccc
      601 cgccctcgcg catcgccgcg gcaaagtcgg ctggctggtt caggtcgcgg ctcacgtaac
      661 ccggcgtgtc ggcattggcg acattctggg cgatgacgcg ctgacgttcg tccagccagg
      721 acaggcggcc cttgatctgg ccgagcagcg gcaggtcggt gatacccacg tgggcgtccc
      781 cttcgggcgc ggatcgccca ttcgagatag gcagtaactg ccgcgtgcat ggttaacggg
      841 acgttatctc aacgcggcgt taaccatgat cgccgaaggc tggggcatga acctcctcga
      901 cctgctgcgg gctgttttcg cgctcgcctt cacactgggc atcatcggcc tggctgcctg
      961 ggccgcgcgg cggtacgcgc cctcgctgct ggcgcgcctc agcgccgagc gcggcgagaa
     1021 gcggatgaag gtggtcgaaa ccctgatgct ggatcccgcc cgtcggctgg tgctggtctc
     1081 catcgacggc gaggagcggc tgctgatgct gggcgaaggc cgcgaactga tcgagccgcg
     1141 cggcgccgtc acgccgtccg gagccgccca gtgaacctca agggcgttct ggtcatgccc
     1201 acgcgcgcag agttgaagcg cgcggcgatc ctgtcgctga tcaccaccct cgcctgcctg
     1261 ctatggccgt tgggcgccct ggcgcaggac atcgccgccg ctggcggacc gggcgcggcc
     1321 atcaacgtcg acctcggcac cggcgcgggc ctgaccgaac gggtggtcca gctggtcggt
     1381 ctgatgaccg tcctgtcact ggcgccgtcg atcgtcatca tgaccaccag cttcgtgcga
     1441 atcgtcgtgg tgctgtcgct gttgcgcact gcccttggca tgcagcagtc gccgccgaac
     1501 gcggttctgg tgtccctggc gctgttcctg agcgccatcg tgatggctcc gacatggcag
     1561 gacgcatatg attcgggcat ccgccccctg ctggatcagc agatggagct gccccaggcc
     1621 ttcgatgcgg cgtcggaacc tgtgaagact ttcatgctgg cccaggtgga ccgggacgac
     1681 ctcgccctgt tcgtccgtct gtcccagttg gaggccccca cggcgaccgc cgacctgccc
     1741 ctgagagtgg tcaccccggc cttcatgatc agcgagttga agaaggcctt cgaaatcgga
     1801 tttctccttt tcgttccgtt ccttgtgatc gatctggtgg tcgccagcgt gctcatgtcc
     1861 atgggtatga tgatgcttcc tccggtggtg atttcgctcc cgttcaagct gatctttttc
     1921 gtgctcgtgg acggctggag actggtcgcc ggaagcctgg tcgagagctt ccagcgagcg
     1981 tccggtaccg ggtaaatccc cagcccgtgg gggttacagc ggcgaagtcg cttgccaagc
     2041 tagcaaaaag cggcgtcaat ccgtccgtca tcgcccctga atcggggcga gctttcatcg
     2101 ggaaacgacc cacatgacca cccaagcaga actcatcgcc tccgtcgcca aggacgccgg
     2161 tgtcagccag gccgacgccg gccgcatgct cgacgccctc gtcaagaaca tccacaagtc
     2221 gctgaccggc ggcggcgacg ttcgcatctc gaacctcggc gtgttcgaca ccgccgcccg
     2281 cgccgcccgt gaaggccgca acccggccac cggcgcgacc atcaagatcg ccgcctcgaa
     2341 ggccgtgcgc ttccgcgttt cgaagccgct caaggatgcg gtgaacaagt aggaccgaaa
     2401 ggtctacgca gccggcctcg caggaggcgc tgcagcagct tgaggacggg cgggggccgc
     2461 agcgcggttc ccgcccgttt tcgttctcag ctcggccttc atggccgtga cccagcccgc
     2521 gaaggtgtcg gccgaggcgg tcaggcccgc gaagtcgccg ggcgccacgc cgtcgtcgtc
     2581 cagggcgatc gacagggccg tcttcgcccg ggccccggcg atgaaggggc ggtagttgcg
     2641 cgacagccgg ttcaggccgg cggcgtcatg gcccagcgaa tagccgacgc cggcgcgaat
     2701 cagccgcgcc tcttcctcgg cggtgagggg cgccggatcg gcgaaccgct ggcccagccg
     2761 ggcctcgtag agggcggcgg cctgcggcca tttctgctgt ttccagaaga tctcggcgcg
     2821 aacctcgcgc gcctccggcg aggcgtcgtc gcccaggatc tccagcgcgt gatcatagcg
     2881 gcccagctgg gtcagggccc gggcttccag cgcccgccgc tcggcgttca gcgggccggg
     2941 cagcagggtg gagcgcgagc cccagatggc ctgcagcgcc ggctccggct ggcggtccat
     3001 cagatagacg gtggccagat cggtggcgac ctgggccttg gccacgcctt ccagccggtt
     3061 ctcgacctga tacttcagca gctcggccgc ctgatccagc agatcgacgt cgaccagccg
     3121 ccgcgccaac cggcgcacca tct
//
LOCUS       DASVVN010000226         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_33503, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000226 DASVVN010000000
VERSION     DASVVN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33503"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF686_03540"
     CDS             <1..186
                     /locus_tag="VF686_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8660916.1"
                     /translation="IGRPLAGDARYGGALTFAGRAAPRLMLHAARLVFPHPEGGYRTV
                     EAPPPWDFEALKAAAGL"
     gene            264..764
                     /locus_tag="VF686_03545"
     CDS             264..764
                     /locus_tag="VF686_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660917.1"
                     /translation="MNRFTLPLIAAAGLTLSACASLAPYGAQMSPGGQGYAEQRIESN
                     RFRVTYNGVGAPGPVADMALLRAAELTTSQGYDWFEVTQSWTDGRPDSAGGFRPSVGV
                     GYGSSHYDGRYGNYSSSGVGLGIGLNFSGPSPTSTSIEVVMGNGAKPDRPNAYDARGV
                     QDALRR"
     gene            complement(909..>1824)
                     /locus_tag="VF686_03550"
     CDS             complement(909..>1824)
                     /locus_tag="VF686_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660918.1"
                     /translation="QPLDLFPFWEDGRKPDRFHVLFNRQIEERFPIDQDIRSHVHTYG
                     RGVFRSLMVHIGVKRLNQALALIAFAALATLGPTWFTGWTNSPQMGLGLAVGAMIVVF
                     GLWWGIQAVLFTQYRFRLENDSYEMSREIVQRTRELQNLFTSARAMADQAETQFQMDG
                     KGWGARAMYLTRLTMWLGARMEYLEKYVQMELWRVRRERYWTRWLQRFATPLVLIVWL
                     VLFALEPVAGDTMTFRGLQGLAILLGVLVSWVSYVRWRTPMEAIQQKLGVEGWVRYAS
                     LDVDNAVGEQVRRDKERLVEYRALTRGR"
BASE COUNT          302 a          668 c          577 g          277 t
ORIGIN      
        1 atcggccgcc cgctggccgg cgacgcccgc tatggcggcg ccctgacctt cgccggccgc
       61 gccgcgccgc gcctgatgct gcacgctgcc agactggtct ttccccaccc cgaaggcggc
      121 taccgcacgg tggaggcgcc gccgccctgg gatttcgagg cgttgaaggc ggcggcggga
      181 ctttaggggg cccgcatcgg ctggcggaca caggaaccac cgccgcgtcg ccgcgctaat
      241 cttcacacgc ccggagaccc gccatgaacc gtttcaccct gcctttgatc gccgccgcgg
      301 gcctgacctt gtccgcctgc gccagcctgg ccccgtacgg ggcgcagatg tcgccgggcg
      361 ggcagggcta tgccgaacag cgcattgaat ccaaccgctt ccgcgtcacc tacaacggcg
      421 tcggcgcgcc cggcccggtc gccgacatgg ccctgctgcg ggcggctgaa ctgaccacct
      481 cgcagggcta tgactggttc gaggtgaccc agagctggac cgacggccgg cccgacagcg
      541 ctggcggctt ccgccccagc gtcggggtcg gctatggctc cagccactac gacggccgct
      601 acggaaacta ttccagctcg ggcgtcggct tgggcatcgg cctgaacttc agcggaccgt
      661 cgccgacctc gacgtctatc gaggtcgtca tgggcaacgg cgccaaaccc gaccggccca
      721 acgcctatga cgcccgcggc gttcaggacg cgctgcggcg ttagacttta tccgtgcatc
      781 cccgcgaagg ggggacccag gctttagcct caccaccgtt gctggaccga gcgcccgttc
      841 attcaaacag cctgccagcc gctcaaacct cggtccccac cctcgcgggg atgccggacg
      901 agagaacctc agcgccctcg cgtcagcgcc cggtattcga ccagccgttc cttgtcgcgg
      961 cggacctgtt cgccgaccgc gttgtcgacg tccagcgagg cgtagcggac ccagccctcg
     1021 acccccagtt tctgctggat ggcctccatc ggcgtgcgcc agcgcacgta ggacacccaa
     1081 ctgaccagca cgcccagcag gatggccagc ccctgcaggc cgcggaaggt catcgtgtcg
     1141 cccgccaccg gctccagcgc gaacagcacc agccagacga tcagcaccag cggcgtggcg
     1201 aaccgctgca gccagcgggt ccagtagcgt tcgcggcgga cccgccacag ctccatctgg
     1261 acgtactttt cgaggtattc catccgcgcg cccagccaca tggtcagccg ggtcagatac
     1321 atggcgcgcg cgccccagcc cttgccgtcc atctggaact gggtctcggc ctgatcggcc
     1381 atggcccggg ccgaggtgaa caggttctgc agctcgcgcg tccgctgaac gatttcgcgc
     1441 gacatctcgt atgaatcgtt ttccagccgg aaccgatact gggtgaacag cacggcctga
     1501 atgccccacc acaggccgaa gacgacgatc atcgcgccga cggccagacc cagacccatc
     1561 tgcggcgagt tcgtccatcc cgtgaaccag gtcgggccca gcgtggccaa ggcggcgaag
     1621 gcgatcagcg ccagcgcctg attcagccgc ttgaccccga tgtgaaccat cagcgaccgg
     1681 aacacgccgc gtccgtaggt atggacgtgg ctgcggatgt cctgatcgat cgggaaacgc
     1741 tcctcgatct ggcggttgaa caggacgtgg aaacggtccg gcttgcggcc gtcctcccag
     1801 aaggggaaca ggtccagcgg ctgg
//
LOCUS       DASVVN010000227         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_166714,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000227 DASVVN010000000
VERSION     DASVVN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166714"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VF686_03555"
     CDS             <1..708
                     /locus_tag="VF686_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8660919.1"
                     /translation="LYRQPRDHPRLGAAELAHIESDPADPPGKVGWRVIAFKRETWAF
                     LLGKFITDPVWWLFLFWLPDFFAKTYDLNILSFGPPLVAVYLLADVGSVGGGWLSSRL
                     IARGWTVNRARKGTMLLCALCVTPISIAIFADNLLVAVGVIGLAAAAHQGWSANLFTL
                     VSDVFPRRAVATVVGFGAAGGAVGGMFIAKFAGWTLETTGSYVPLLAFAGAAYLIALG
                     IIHLLTPRLAPVTQPPE"
     gene            769..1854
                     /locus_tag="VF686_03560"
     CDS             769..1854
                     /locus_tag="VF686_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8660920.1"
                     /translation="MKPRSTRRKGGGHTIDDVARHAGVSPMTVSRVINDERNVRDTTR
                     KRVTDAIRELGYAPNQAARSLASAQTLKIGLLYSNPSAAYLSEFLLGGLDQCSRSGIQ
                     LIVEKCEPEVGEIVAIERLLASGVDGVLLPPPLCDSSIVIERLKAAGLPAVAVASGDP
                     LPSVSAIRIDDLAAAQAMTRHLIGLGHRRIAFVRGHPNQTASRLRYEGYAKALGEHGL
                     TVDDRLVVQGYFTYHSGLDAADKLLNLRHRPTAIFASNDDMAAAAIAVAHRQGLDVPR
                     DLTIVGFDDTALASSVWPPLTTIRQPVAEMSREAVSILADAIRRRIDGQPPAATSELM
                     DFALIRRQSDGHPPTGDDGDAPGVSAA"
BASE COUNT          287 a          705 c          661 g          338 t
ORIGIN      
        1 ctgtatcgcc agccccggga ccatccgcga ctgggcgcgg ccgaactcgc ccacatcgaa
       61 agcgaccccg ccgatccgcc gggcaaggtc ggctggcggg tcatcgcctt caagcgcgag
      121 acctgggcct tcctgctggg caaattcatc accgatccgg tgtggtggct gttcctgttc
      181 tggctgccgg acttcttcgc caagacctat gacctgaaca tcctttcctt cggaccgccg
      241 ctggtggcgg tctatctgct ggcggacgtc gggtcggtgg gcggcggttg gctgtcgtcg
      301 cggctgatcg cacgcggctg gaccgtgaac cgggcccgca aggggaccat gttgttgtgc
      361 gccctgtgcg tgaccccgat ctcgatcgcc atcttcgccg acaatctgct ggtggcggtg
      421 ggcgtcatcg gtctggcggc ggcggcgcat cagggctggt cggccaatct gttcaccctg
      481 gtgtcggacg tctttccccg acgcgccgtg gccaccgtcg tcggcttcgg cgccgcgggc
      541 ggcgctgtgg gcgggatgtt catcgccaaa ttcgccggtt ggacgctgga gacgacgggc
      601 agctatgtcc cgctactggc cttcgccgga gcggcctatc tgatcgcgct cggcatcatc
      661 cacctgctga ccccgcggct ggcccctgtg acccagccgc ccgaatgacg ccatgcctcg
      721 ctctggaaag cggcgggcgc tagggtgccg gttctgctgg agagaagatt gaagccgaga
      781 tcgacaagac ggaagggcgg cggtcacacc atcgacgacg tcgcccggca cgcgggcgtt
      841 tcgccgatga cggtgtcgcg cgtcatcaac gacgaacgca atgtccgcga caccactcgc
      901 aagcgggtca cggacgccat tcgcgaactg ggctatgcgc cgaaccaggc cgcgcgcagt
      961 ctggccagcg cccagacgct gaagatcggc ctgctctaca gcaacccgtc cgccgcctat
     1021 ctcagcgagt tcctgctggg ggggctcgat cagtgcagcc gctcgggcat ccagcttatc
     1081 gtcgagaaat gcgagcccga ggtgggcgag atcgtcgcca tcgaacggct tctggccagc
     1141 ggcgtggacg gggtgctgct gccgccgccg ctctgcgatt ccagcatcgt catcgaacgg
     1201 ctgaaggccg cgggcctgcc cgccgtcgcc gtggcctcgg gcgatcccct gcccagcgtg
     1261 tcggccatcc gcatcgacga cctggccgcc gcccaggcca tgacccgtca cctgatcggt
     1321 ctgggtcatc gccgtatcgc cttcgtgcgc ggccacccca accagaccgc cagccgcctg
     1381 cgctatgagg gctacgccaa ggcgctgggc gaacacggcc tgacggtgga cgaccgcctc
     1441 gtggtccagg gctatttcac ctaccactcg ggtctggacg cggcggacaa gctgttgaac
     1501 ctgcgccacc gtccgaccgc catcttcgcc agcaacgacg atatggccgc cgccgccatc
     1561 gccgtggccc accgtcaggg tctggacgtg ccgcgggatc tcaccatcgt cggtttcgac
     1621 gacacggcgc tggccagcag cgtctggccg ccgctgacca ccattcgaca gccggtcgcc
     1681 gaaatgtcgc gggaggccgt gtcgatcctg gccgatgcga tccgccgcag gatcgatggc
     1741 cagcctcccg ccgcaacgtc cgagctgatg gatttcgccc tgatccggcg gcagtcagac
     1801 ggccatccgc cgacgggcga cgacggcgat gcgcccgggg tgtccgccgc ctgaaccgca
     1861 cggcttcgca aagggcgatg ggcgaggtac ttgtcggaca tttcctgctg gagcggctca
     1921 tgtccctcac ccgccgacgc cccgtcacgg tttcggccgc cgcggcgttc gcgcccgcgg
     1981 gcgcggctct g
//
LOCUS       DASVVN010000228         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_140292,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000228 DASVVN010000000
VERSION     DASVVN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140292"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..860
                     /locus_tag="VF686_03565"
     CDS             123..860
                     /locus_tag="VF686_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660921.1"
                     /translation="MTEAAKPMEDHVLVEVTVPAPADAVWAALRDPQQIKNWFGWDAD
                     SLKDEIDFIFVTHAEADEARRTVTFVGVNDRYEVEARGETSVVRVVRSAPVGEWTDVF
                     DGMIEGWISFTWQLAFAFARHPGQTRRTIFLSGPPRADQLGRSLLGLDAAPAPGEPYS
                     MPLGANADAAGQVWFTARHQIGVTVDAWGDGLLVVLDQPPTEKKPRGSTMLTLTTYGL
                     SDAAFADLERRWKAWWDQRFETVAHDC"
     gene            complement(864..1352)
                     /locus_tag="VF686_03570"
     CDS             complement(864..1352)
                     /locus_tag="VF686_03570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660922.1"
                     /translation="MVHVRKATEADWPEIWPIVKATAAAGETYTYPRDLTEAFGKALW
                     NPPTWVVLVAVDETGAVLGTAKFGPNQLGPGSHIANASFMVSPEARGRGVGWALGEAV
                     IRAAAEAGYHAMQFNAVVEANRAAVKLWRSLGFEIIGTAPEAFDHPTEGLVGLHLMYR
                     RL"
     gene            complement(1352..>4189)
                     /gene="mfd"
                     /locus_tag="VF686_03575"
     CDS             complement(1352..>4189)
                     /gene="mfd"
                     /locus_tag="VF686_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8660923.1"
                     /translation="VSLAPVSEALLDPDSISRFRTGYLSLFGAAGDDPLYAAISEGAR
                     RQGMEHWLPLLFDRLETLFDYLPEDAKVFLDNQVEAARAERWTLTRDAYEARRDAAIS
                     RKAGGSAYRALPPERLYLDEGDWNSALAGRWVRRMSPFQGPGEDAGGRLGRSFAAERA
                     QDSVNLFEAVARHAETVKAAGKRVLFASWTEGSSERLAAMLADHGLEHVVAVRDWADV
                     QKAPRDIYLRGVLPVEHGFETDQVAVISETDILGDRLARPRKKRRASNFLAEASALTT
                     GDLVVHLDHGIGRYEGLKTLDIQQAPHDCLELLYAGDSKLYLPVENIDLLTRYGSDAD
                     GAQLDRLGGAGWQARKAKAKARLRDMAEGLIALAAKRSLRSSDPITPPQGLFDEFCAR
                     FPYEETDDQLNAIGDVLEDLGKGTPMDRLICGDVGFGKTEVALRAAFVVAMSGQQVAI
                     VCPTTLLARQHFKTFSERFAGWPVKVRHLSRMVTAKEAAETRAGLKDGSFEIVVGTHA
                     VLSEQVGFKDLGLVIVDEEQHFGVKHKEKLKSLRADVHLLTLTATPIPRTLQMALSGI
                     REMSIIATPPVDRLAVRTYVTPWDPVLVREALLREKYRGGQAYYVVPRLSDLPDIEKF
                     LREQVPEIKFVVGHGQMSPTQLEDVMSAFYDGSYDLLLSTTIVESGLDVPTANTLIVH
                     KADMFGLAQLHQIRGRIGRAKARAFAYLTTPPEKPMSLSSERRLQVLQSLDNLGAGFQ
                     LASHDLDQRGGGNLLGDEQSGHIREVGVELYQQMLEDAVAELRQAGEGGGVVDRGWSP
                     AINVGAAVLIPEDYVPDLNVRLSLYRRLSDAENSETREALAAELIDRFGPLPDEAKQL
                     LRIVGIKANCKTACIERIDIGPKGAVLTLRNNEFPNPMGLVGLIQKNQAFWKIRPDQK
                     IVVKGEWETAADRLKVAERITADLARVAGAA"
BASE COUNT          641 a         1552 c         1321 g          675 t
ORIGIN      
        1 gtcagcgctg ccgtcgccgc tctggcccgc aagtattcgg cgtccacggg ccgccgctac
       61 cgcctcaccg ccgccgccca cccggcgacg ccccgacccg ctccagccag gaactgaccg
      121 ccatgaccga agccgccaag cccatggaag accacgtcct cgtcgaggtc accgtgcccg
      181 cgcccgccga tgcggtctgg gccgcgctgc gcgacccaca gcagatcaaa aattggttcg
      241 gctgggacgc cgacagtctg aaggacgaga tcgacttcat cttcgtcacc cacgccgagg
      301 ctgacgaagc ccgccgcacc gtcaccttcg tgggcgtcaa cgaccgctac gaggtcgagg
      361 cgcgcgggga gaccagcgtc gtccgcgttg tccgctccgc tcccgtcgga gagtggacgg
      421 atgtgttcga cggcatgatc gagggctgga tttccttcac ctggcaactg gccttcgcct
      481 tcgcccgcca ccccggccag acgcgccgca cgatcttcct ctcgggcccg ccgcgcgcgg
      541 accagctggg ccgttcgctg ctcggcctcg acgccgcccc ggctccgggc gagccgtact
      601 cgatgccgct gggcgccaat gcggacgccg ccggtcaggt ctggttcacc gcgcgtcatc
      661 agatcggcgt caccgtcgac gcttggggag acggcctgct ggtcgtgctg gaccaaccgc
      721 cgacggagaa aaaaccgcgg ggctccacca tgctgaccct gacgacctac ggcctgtccg
      781 acgcggcctt cgccgatctg gagagacgct ggaaggcgtg gtgggatcag cgcttcgaga
      841 ccgtcgccca cgactgctga gcctcagagc cggcggtaca tcaggtgcag accgacaagg
      901 ccttcggtcg ggtgatcgaa ggcctccggc gccgtgccaa tgatctcgaa acccagcgac
      961 cgccacagct tcaccgccgc gcggttcgct tccaccacgg cgttgaactg catggcgtga
     1021 tagccggcct ccgccgcggc gcggatcacc gcctcgccca gcgcccagcc tacgccccgt
     1081 ccacgcgcct ccggcgagac catgaagctg gcgttggcga tatgggagcc gggccccagc
     1141 tggttcggac cgaatttcgc cgtacccagc accgcgccgg tttcgtcgac cgcgaccagc
     1201 accacccacg tcggcggatt ccacagcgcc ttgccgaagg cctcggtcag gtcgcgcgga
     1261 taggtgtagg tctctccggc ggccgccgtc gccttcacga tcggccagat ctccggccag
     1321 tccgcctcgg tcgccttgcg gacgtgaacc atcaggccgc cccggctacc cgcgccagat
     1381 cggcggtgat gcgttcggcg accttcagcc tgtccgccgc cgtctcccat tcgcccttca
     1441 ccacgatctt ctggtccggc cgaatcttcc agaaggcttg gttcttctgg atcagaccga
     1501 ccagccccat cggattgggg aattcgttgt tgcgcagcgt cagcaccgcg cccttgggcc
     1561 cgatgtcgat gcgttcgata caggccgtct tgcagttggc cttgatgccg acgatgcgca
     1621 gcagctgttt cgcctcgtcc ggcaacggcc cgaaccggtc gatcagttcg gcggccagag
     1681 cctcgcgcgt ttccgagttc tcggcgtcgg acagacggcg atacaggctc agacgaacgt
     1741 tcaggtccgg gacgtaatcc tctggaatca atacggccgc gccgacgttg atggccggcg
     1801 accagccgcg atcgacgacg cccccgccct cgcctgcctg acgcagttcc gcgaccgcgt
     1861 cctccagcat ctgctggtac agctcgaccc cgacctcgcg gatatggccc gactgttcgt
     1921 cgcccagcag gttgccgccc ccgcgttgat ccagatcgtg gctggccagc tggaaaccgg
     1981 cgccgagatt gtccagcgac tgcagcacct gcagccgccg ttccgaagac aggctcatcg
     2041 gcttctcggg cggagtggtc agataggcga aggcgcgcgc cttggcccgg ccgatccggc
     2101 cgcgaatctg gtgcagctgg gccaggccga acatgtcggc cttgtgcacg atcagggtgt
     2161 tggccgtcgg cacgtccagt ccgctctcga cgatcgtcgt cgacagcagc aggtcgtagc
     2221 tgccgtcgta gaaggcgctc atcacgtctt ccagctgggt cggggacatc tggccgtggc
     2281 cgaccacgaa cttgatctcg gggacctgct cgcgcaggaa tttctcgatg tccggcaggt
     2341 ccgacaggcg cggcacgaca taataggcct gcccgccgcg gtatttctcg cgcagcaggg
     2401 cttcgcggac cagcaccggg tcccacggcg tcacataggt ccgcaccgcc agacgatcga
     2461 ccggcggggt ggcgatgatc gacatctcgc ggatgcccga cagcgccatc tgcagcgtgc
     2521 gcgggatcgg ggtcgccgtc agggtcagca ggtgaacgtc ggcccgcagg gacttcagct
     2581 tctccttgtg cttgacgccg aagtgctgct cctcgtcgac gatcacaagg cccaggtcct
     2641 tgaacccgac ctgttcgctc agcaccgcat gggtgcctac gacgatctcg aacgatccgt
     2701 ccttgaggcc cgcccgcgtc tctgccgcct ccttggccgt caccatccgc gacaggtggc
     2761 gcaccttgac cggccagccg gcgaaccgct cgctgaaggt cttgaaatgc tgccgcgcca
     2821 gcagggtcgt cgggcagacg atggccacct gctggccgct catggccacc acgaaggccg
     2881 cgcgcagcgc cacctcggtc ttgccgaagc cgacgtcgcc gcagatcagg cgatccatcg
     2941 gcgtgccctt gccgaggtcc tccagcacgt cgccgatggc gttcagctgg tcgtccgtct
     3001 cctcgtaggg gaagcgggcg cagaactcgt cgaaaagccc ctgcggcggc gtgatcgggt
     3061 cggaactgcg caggctgcgc ttggccgcca gggcgatcag cccctctgcc atgtcgcgca
     3121 gccgggcttt cgccttcgcc ttgcgcgcct gccagcccgc gccgcccagc cggtccagtt
     3181 gcgccccgtc ggcgtccgaa ccgtagcgcg tcagcaggtc gatgttctcg accggcagat
     3241 acagcttgct gtcgccagcg tagagcagct ccagacagtc gtgcggcgcc tgctggatat
     3301 ccagcgtctt cagcccctca tagcgcccga tgccgtgatc aagatggacc accagatcac
     3361 cggtcgtcag cgccgaggcc tcggccagga agttcgacgc ccgccgcttc ttcctcggcc
     3421 gcgccagccg gtcgccgagg atgtcggttt cggagatgac cgcgacctga tcggtctcga
     3481 agccatgctc caccggcagg acgccgcgca gatagatgtc cctgggcgcc ttctggacat
     3541 ccgcccagtc gcgcaccgcg accacatgct ccagcccgtg atcggccagc atagcggcca
     3601 gccgctcgga cgagccctcg gtccatgagg cgaacagcac ccgcttgccc gccgccttca
     3661 ccgtctccgc atgacgcgcc acggcctcga acaggttgac gctgtcctgc gcccgctcgg
     3721 ccgcgaagct gcgccccagc cggccaccgg cgtcctcgcc cggtccctgg aacggcgaca
     3781 tccgccgcac ccaccgcccg gccagggcgc tgttccagtc gccctcgtcc agatagagcc
     3841 gctcgggcgg cagggcgcga taggcagacc cgcccgcctt ccggctgatc gccgcgtcgc
     3901 gtcgcgcctc ataggcgtcg cgcgtcaggg tccagcgttc ggcccgcgcc gcctcgacct
     3961 gattgtcgag gaacaccttc gcgtcctcgg gcagatagtc gaacagcgtc tccagccgat
     4021 cgaagagcag cggcagccag tgctccatgc cctgccgccg cgcgccctcg ctgatggcgg
     4081 cgtacagcgg atcgtcgccc gccgcgccga agaggctcag atagccggtg cggaaccgcg
     4141 agatgctgtc cgggtccagc agggcctccg acaccggcgc cagcgaaac
//
LOCUS       DASVVN010000229         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_166770,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000229 DASVVN010000000
VERSION     DASVVN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166770"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VF686_03580"
     CDS             complement(<1..463)
                     /locus_tag="VF686_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX8660924.1"
                     /translation="MNWDLVWPVAAAMTAGGLIGIERTYHGHPAGFRTHILVCLTSCL
                     LMLAAMHQATWAFTALPGQTVVIDPTRMSHGLLTGIGFLCAGVIFREGFSVHGLTTAA
                     SLWVTSAIGVLFGVGMLDLALIGSGATLAVLAVLRLLDAKLPHIGVMDITLR"
     gene            complement(544..1236)
                     /locus_tag="VF686_03585"
     CDS             complement(544..1236)
                     /locus_tag="VF686_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX8660925.1"
                     /translation="MTDLPGFALPLLAAVVAGGLIGFEREWRGRAAGFRTHILVCLAS
                     ALLMEAAVSQGAWRFLPMPGTEIVSDPTRMAHGVLTGIGFLCAGVIFRSGFSIHGLTT
                     AASLWITAALGLTFGAGLFALGLTGTVLTLAILVALRLVSLRLPQKSVVDAEVVWSRG
                     DLEAEARIEAALESVDRARRADRFELIDGGDSLRRSFRLKLDNEAALKDLALRLRAIE
                     GVMAYRLDPRDD"
     gene            1382..1588
                     /locus_tag="VF686_03590"
     CDS             1382..1588
                     /locus_tag="VF686_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660926.1"
                     /translation="MQSPTDRCLDVENYSALKAEVLRAAVELSDEQLMDLTDALHVVI
                     RFRRPGRPIRNPDGWVTDELRSFA"
     gene            complement(1597..2166)
                     /locus_tag="VF686_03595"
     CDS             complement(1597..2166)
                     /locus_tag="VF686_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8660927.1"
                     /translation="MGPWDPAPPEVEAPAPEPRRRGSRIGVWLVVIAALIAFAAFVLA
                     NFRFTLGEVSYSDEAVDVEIIEPLPRPAPEPRPSAPPAPPPDPEPAVGPNGEVLRHPV
                     WLRQPAPDFPTMAMRRGVEEGAVVLRCETFATGEFGACEALSETPGGAGFADAALASM
                     RQARVKPYSIDGFETDSEIQFTVRFRMAP"
     gene            complement(2167..2940)
                     /locus_tag="VF686_03600"
     CDS             complement(2167..2940)
                     /locus_tag="VF686_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8660928.1"
                     /translation="MAGHSHAKNIMHRKAAQGAKKARAFGKLIREITVSAKQGLPDPA
                     MNPRLRLAVNNAKAESLPRDVIQRAINKAAGGDVDTMEEVRYEGRGPGGVGIIVEALT
                     DNRNRAASNIGAAFKKNGGQLSEMNSVAFMWNRVGRIIYPAEAGTEDAVMEAAIEAGA
                     QDVESDLVKPDIYEDAPGHTIWTTFEDLNEVAEALSKILGDAKSTALVWRPQNEVPVT
                     GEAVGTLMKLLDALDAEDDVQNVYSNEDIPDEDLAKYAG"
BASE COUNT          490 a         1124 c         1024 g          489 t
ORIGIN      
        1 agcgcagggt gatgtccatc accccgatgt gcggcagttt ggcgtccagc aggcgcagca
       61 ccgccagcac cgccagcgtg gccccggacc cgatcagcgc caggtccagc atcccgaccc
      121 cgaacagcac gccgatcgcc gaggtgaccc acaacgacgc cgccgtggtc aggccatgga
      181 ccgagaaccc ctcgcggaag atcaccccgg cgcacaggaa gccgatgccg gtcagcaggc
      241 cgtgcgacat ccgcgtcggg tcgatcacca ccgtctggcc gggcagggcc gtgaaggccc
      301 aggtcgcctg atgcatggcc gccagcatca gcaggcagga ggtcaggcac accaggatgt
      361 gggtccggaa tcccgccgga tgcccgtgat aggtgcgctc tatgccgatc aggccgccgg
      421 ctgtcatggc cgccgcgacg ggccagacca gatcccagtt catgtgtgta aatcgctccg
      481 aagactgacg aatctccaac gtagacccgg gagtcgttcg cgatcaagcg ggcgccggct
      541 tcgtcagtcg tcgcggggat ccagcctgta ggccatgacg ccctcgatgg cccgtaggcg
      601 cagggccaga tcctttagcg cggcctcgtt gtccagcttg aggcggaagc tgcgacgcag
      661 actgtctccg ccgtcgatca gttcgaaccg gtccgcgcgc cgggcccggt cgacgctctc
      721 cagcgccgcc tcgatccgcg cctcggcctc caggtccccc cggctccaga ccacctcggc
      781 gtcgaccacc gacttctgcg gcagcctcag gctgaccagc cgcagcgcca ccagaatggc
      841 gagtgtcagg accgtcccgg tgaggccgag agcgaacagg cccgccccga acgtgaggcc
      901 gagcgcggcg gtgatccaca gcgaggcggc cgtcgtcagg ccgtggatcg agaagccgga
      961 ccggaagatc accccggcgc acaggaagcc gatgccggtc aggacgccgt gggccatgcg
     1021 ggtcggatcg ctgacgatct cggttccggg catcggcagg aaccgccacg ccccctgcga
     1081 aaccgccgcc tccatcagca gggccgaggc cagacagacc agaatgtggg tgcggaaccc
     1141 cgccgccctg ccccgccatt cgcgctcgaa cccgatcagc ccgccggcca cgacggcggc
     1201 cagcagaggc agggcgaaac cgggaaggtc ggtcatgtcc atgcagcggc aaacggtcgg
     1261 gcctgccacg ctgttccgcc tgaaggccgc gccggggccg cgaaggccgc ggggaacttc
     1321 tgtcggctat ccgacgttag gaccctttgg cggccgtctc cggtcgcccc gggaggttgc
     1381 aatgcaaagc ccgactgacc gctgtctcga tgtcgagaac tactcggccc tgaaggcaga
     1441 ggtgctgcgc gccgccgtcg aactgtccga tgagcaactg atggatctga ccgatgccct
     1501 gcacgtcgtg atccgcttcc gccgccccgg ccggccgatc cggaaccccg acggctgggt
     1561 taccgacgag cttcgcagct tcgcctgatc gtcacgttac ggcgccatcc ggaagcggac
     1621 ggtgaactgg atttcgctat cggtttcgaa cccgtcgatg ctgtagggct tcacgcgggc
     1681 ctgccgcatt gacgccagcg ccgcgtcggc gaacccggcg ccccccgggg tctcgctcag
     1741 agcctcgcac gctccgaact ctcccgtcgc aaaggtctcg catcgcagca cgacagcgcc
     1801 ctcctcgacg ccgcgacgca tcgccatggt cggaaaatcc ggcgcgggct gtctcagcca
     1861 caccgggtgc ctcaacactt cgccattggg gccgacggcc ggctccggat caggcggagg
     1921 cgcgggtggc gcgctcgggc gcggttcagg ggcgggtcgc ggaagcggct cgatgatttc
     1981 cacgtccacc gcctcatccg agtagctgac ctcgccaagg gtgaagcgga agttggccag
     2041 aacgaacgcc gcaaaggcga tcagggccgc aatgacgacc agccagaccc cgatgcggga
     2101 accgcgccgc cgaggctccg gcgcaggagc ttcgacttcg ggcggcgcag ggtcccacgg
     2161 tcccattcag cccgcgtact tcgccagatc ctcgtccggg atatcctcgt tcgaatagac
     2221 gttctgcacg tcgtcctcgg cgtccagcgc atccagcagc ttcatcagcg tgccaaccgc
     2281 ctcgcccgtc acaggcacct cgttctgcgg ccgccagacc agcgccgtcg acttcgcatc
     2341 gccgaggatt ttcgacaggg cctcggccac ttcgttcaga tcctcgaagg tcgtccagat
     2401 ggtgtggccc ggcgcgtcct cgtagatgtc gggcttgacc agatcgctct cgacgtcctg
     2461 ggcgccggcc tcgatggcgg cctccatgac ggcgtcttcg gtgccggcct ccgccggata
     2521 gatgatccgg ccgacccggt tccacatgaa ggcgaccgag ttcatctcgc tcagctgccc
     2581 gccgttcttc ttgaacgccg cgccgatgtt ggaggcggcg cgattgcggt tgtccgtcag
     2641 ggcctcgacg atgatgccca cgccgccggg gccgcggccc tcgtagcgga cctcttccat
     2701 cgtatcgacg tcgccgcccg cggccttgtt gatggcgcgc tggatcacgt cgcgcggcag
     2761 gctttcggcc ttggcgttgt tcaccgccag acgcaggcgc gggttcatgg ccgggtccgg
     2821 cagcccctgc ttggcggaaa ccgtgatttc gcggatcagc ttgccgaagg cgcgtgcctt
     2881 cttggcgccc tgggccgcct tgcggtgcat gatgttcttg gcgtgcgaat ggccggccat
     2941 ggccgcaccc tcttgcttcc ggagccggcc aaccgctgcc cgaggccccg acgcccctgg
     3001 gccagcccct ggccttgctc ctcagattcc ggttgaggat ggtcttatag cgagccgtcc
     3061 cgccgggcgc cactggtgga acacatccgc gtgcgcgagc cccctcctgc ccctggcggg
     3121 cgtggtg
//
LOCUS       DASVVN010000230         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_33558, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000230 DASVVN010000000
VERSION     DASVVN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33558"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF686_03605"
     CDS             <1..311
                     /locus_tag="VF686_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660929.1"
                     /translation="QDVFKAFSRTLDSSSKAGSKEEGTASKESPDVVAALRVLSKAPT
                     GHMALPEFADLLPGDQMSSLTAAIELMKRGLITMDTQERNAVRITDVGRTVSGLTSSA
                     "
     gene            308..1039
                     /locus_tag="VF686_03610"
     CDS             308..1039
                     /locus_tag="VF686_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660930.1"
                     /translation="MSPGTVTMVDWWPGDLWPGYVVAALVSAAVGVADILARYKDDPW
                     QAIRTRASAGYVVANLVFGLAAFWFAIVLDLVDVRSADGPVDPLRDGLLVGFAAVLVL
                     RAVAFNINVGGKTDQVGPSAIVDTLLAACDQGIDRKLACQKDKQVRTMMEGVSFNAAK
                     TFLPVYCLSLMQNTEGTADRIAQLIGSIEQVPDLTDEQRAYLLGNTLIAVFGAVVAGT
                     AVNGFKPALQAQAQALGQPPPPPAA"
     gene            complement(1036..1227)
                     /locus_tag="VF686_03615"
     CDS             complement(1036..1227)
                     /locus_tag="VF686_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660931.1"
                     /translation="MNTESVVHDDDETSRPLVQWYPRAGGGGAADAVVIAGLALGLFA
                     IAAGVTTALILSRRAQGED"
     gene            1354..>1619
                     /locus_tag="VF686_03620"
     CDS             1354..>1619
                     /locus_tag="VF686_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8660932.1"
                     /translation="MAGHSKFKNIMHKKGRQDAARSKLFSKLSRDITVAAKTGLPDPA
                     MNPRLRLAVANARAESMPKDNIERAIKKGAAGGGEEYEEVRYEG"
BASE COUNT          279 a          556 c          528 g          256 t
ORIGIN      
        1 ttcaggatgt attcaaagcg ttctccagaa cgctggacag ttcatcgaaa gctggatcca
       61 aagaggaggg cacggcttcc aaggagagcc cggacgtcgt tgccgcgttg cgcgtgttaa
      121 gtaaggcccc aacgggccat atggccctcc ctgaattcgc agatttgctg ccgggtgatc
      181 agatgagttc gctcacggcg gcgatcgaac tgatgaaaag ggggctgatc accatggata
      241 cccaggagag gaacgccgtc aggatcaccg acgtcggccg tacggtgtcc gggctgacct
      301 cgtcggcgtg agccccggaa cggtcaccat ggtcgattgg tggccggggg atctctggcc
      361 cggctacgtt gtcgctgcgc tggtgtccgc agccgtcggc gtcgccgaca tcctggcccg
      421 ctacaaggac gatccctggc aggcgatccg cacgcgcgcc tcggcgggct acgtcgtcgc
      481 caatcttgtc ttcgggctcg cggccttctg gttcgccatc gtgctggacc ttgtcgacgt
      541 gcgaagcgct gacggcccgg tcgaccccct ccgtgacggg ctgctggtcg gcttcgccgc
      601 cgtgctcgtg cttcgcgccg tcgccttcaa catcaacgtc ggcggcaaga ccgatcaggt
      661 gggtccgagc gccatcgtcg acaccctgct cgccgcctgt gaccagggta tcgaccgcaa
      721 gctcgcgtgc cagaaggaca agcaggtgcg cacgatgatg gagggggttt cgttcaacgc
      781 ggcgaagacc ttcctgccgg tctattgcct ctccctgatg cagaacaccg aggggacagc
      841 cgatcggatc gcccagctga tcggctcgat cgagcaggtg cccgacctga ccgacgagca
      901 acgcgcctac ctccttggca acacgctgat cgcggtcttc ggagccgtgg tcgcggggac
      961 cgccgtcaac ggcttcaagc ccgcgcttca ggcccaggcg caggccttgg gccaaccgcc
     1021 gccgccgccg gcggcctagt cctcgccctg cgcccggcgg ctcaggatca gcgccgtcgt
     1081 gaccccggcg gcgatggcga acaggccgag cgcgagtccc gcgatcacga ccgcatcggc
     1141 cgccccgccg ccgcctgccc gcgggtacca ttgcaccagc gggcgcgatg tttcgtcgtc
     1201 gtcgtgcacg accgactcgg tgttcatgaa gcctccgcgt cctcaacctg aacaacaggc
     1261 tggagctttg ccggttccgc aggggatcgg ctacaagccc cgcctgaccc cgccggcgcc
     1321 ctcgccggcc cagaccaatg acaagacgga ccgatggccg gccactcaaa gttcaagaac
     1381 atcatgcaca agaagggccg ccaggatgcg gcccgctcga agctcttctc caagctgagc
     1441 cgggacatca ccgtggccgc caagacgggc ctgcccgacc cggccatgaa cccgcgcctc
     1501 aggctggcgg tggccaacgc ccgcgccgag agcatgccca aggacaacat cgagcgcgcg
     1561 atcaagaagg gcgcggccgg gggcggcgag gagtacgagg aggtccgcta cgagggctt
//
LOCUS       DASVVN010000231         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_402624,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000231 DASVVN010000000
VERSION     DASVVN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402624"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1219
                     /gene="hutI"
                     /locus_tag="VF686_03625"
     CDS             8..1219
                     /gene="hutI"
                     /locus_tag="VF686_03625"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX8660933.1"
                     /translation="MRVDLLITDCGLATMVEGGEAYGAIVDGAILIREGRIVWIGARS
                     DLPEHTAAATDRLDGRWVTPGLIDCHTHLVFGGDRSGEFEQRLNGATYEEIARAGGGI
                     VSTMAATRAASEDELYASARARLAGLKATGVTTVEIKSGYGLDRDSELKMLRVARRIG
                     REAGVRVRTSYLGLHAVPPEHRADRAAYVDLAIDDILPAAHAEGLVDAVDAYCEPIAF
                     TVEETARLFDKARALGLPVKLHADQLSDGGGAALAGRYGALSADHVEHTTEAGVRAMA
                     EGGVVAVLLPGAYLMLRETTPPPIDLFRAHGVAMAVATDCNPGTSPVASMTAAITLAC
                     VQFRLTPEEALVGATRHAARALGLGDELGTLEAGKAADLAVWDITRPAELAYWLGKPL
                     LERTYVAGERR"
     gene            complement(1506..2123)
                     /locus_tag="VF686_03630"
     CDS             complement(1506..2123)
                     /locus_tag="VF686_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8660934.1"
                     /translation="MTRPPLPRFALGDPLPAPTSSDELRLRLATRRSAPAQALTAPGP
                     SAEELEDILTLGVRSPDHGKLFPWRLVVLGRQTRAGLAEALTPLAAVQTDPNKAKAVL
                     GKLTAAPVTIMVVSAPVPSQKVPEWEQQLSAGAVCMNLEHAANALGYASSWITDWYSY
                     DPQALDILDVEGGEQVAGFIHIGTLAETPLERPRPELADRVTYRP"
     gene            2207..2857
                     /locus_tag="VF686_03635"
     CDS             2207..2857
                     /locus_tag="VF686_03635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8660935.1"
                     /translation="MTDSRTAFDADRPLPVWDDGSDRPPPWSDHGSSILFESPWMRLT
                     RHDATAPTGLKADYTVMRPRNMSVGVLPVHDDGTVTLVGQQRFALMNWSWEMPEGGAP
                     FDEDPIEGARRELAEEAGLAAATWLPAYKAEMANSITDERAMAWIAWGLTPVPVAPDP
                     TEIIRVVRVPFGDLLREIGTGAIRDMFTLATALRAYHMAREGELPPELAKAMLASV"
     gene            2878..>3415
                     /locus_tag="VF686_03640"
     CDS             2878..>3415
                     /locus_tag="VF686_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX8660936.1"
                     /translation="MPKLEVVADRREPWALLRAAAEETIRAEPHLASQLNAVILSHND
                     LASALSFQIARKLGDGELGPMSVREICRDAFDADPKIVASAEADLQAVAERDPAIKTL
                     LQPFLYFKGFQALQAHRVAHWLWGQGREALAFHFQSRISELFQVDIHPAARMGSGLFL
                     DHGTGIVIGETAVVGDEVS"
BASE COUNT          501 a         1090 c         1313 g          511 t
ORIGIN      
        1 aaaagtgatg cgtgtcgatc tcctgatcac cgactgcggc ctggccacca tggtcgaggg
       61 cggcgaggcc tatggcgcga tcgtggacgg ggcgatcctg atccgcgagg ggcgcattgt
      121 ctggatcggg gcacgctctg acttgccgga gcatacggcg gccgcgacgg accggctgga
      181 cgggcgctgg gtcacgccgg ggctgatcga ctgccatacc catctggtgt tcggcggcga
      241 ccggtcgggc gagttcgaac agcggctgaa cggcgcgaca tacgaggaga tcgcgcgggc
      301 cgggggcggc atcgtttcga ccatggcggc gacgcgggcg gcgtcggagg acgagctcta
      361 cgccagcgcg cgggcgcggc tggcggggct gaaggcgacc ggcgtcacca cggtcgagat
      421 caagtcgggc tatggcctgg accgcgacag cgagctgaag atgctacgtg tggcgcggcg
      481 gatcgggcgc gaggcggggg tgcgggtgcg gaccagctat ctgggcctgc atgcggtgcc
      541 gccggagcat cgggcggacc gggcggccta cgtcgatctg gccatcgacg acatcctgcc
      601 ggcggcccat gccgagggcc tggtcgacgc ggtcgacgcc tattgcgagc ccatcgcctt
      661 caccgtcgag gagacggcgc gcctgttcga caaggcgcgg gcgctgggcc tgccggtcaa
      721 gctgcatgcg gaccagctgt cggacggcgg cggggcggcg ttggcggggc ggtacggggc
      781 cctgtcggcg gaccatgtcg agcacacgac cgaggcgggc gtacgggcca tggccgaggg
      841 cggcgtggtc gcggtgctgc tgccgggggc ctatctgatg ctgcgcgaga ccacgccgcc
      901 gccgatcgat ctgttccggg cgcacggggt ggcgatggcg gtggcgacgg actgcaatcc
      961 gggcacctcg ccggtggcct cgatgacggc ggcgatcacc ctggcctgcg tgcagttcag
     1021 gctgacgccg gaggaggcgc tggtgggggc gacgcggcat gcggcgcggg cgctgggact
     1081 gggcgacgag ctcggcacgc tggaggccgg caaggcggcg gatctggcgg tctgggacat
     1141 cacccggccc gccgaactgg cctactggct gggcaagccg ctgctggagc ggacctatgt
     1201 cgcgggagag cggaggtagc cgaatccctg tatcctccgc ggaggagagc gaaatgagcc
     1261 gacgcccgga cgctatcgaa tgcctgctga tcgccgccct cgctgcgacg acgctgctga
     1321 tgggcgtggc gacggtgctg ttgaccgcca gcctgttccg ctagggtcac attccgccag
     1381 catccatctg attccgtcat tccggggacg cgcaggttct gctcccggct gcgccggtcc
     1441 cagccccgga atgacgggat gagggcagaa cagcgcccat gactggatgt cgatcgaatc
     1501 cagctctagg gccggtaggt gaccctgtcg gccagttcgg ggcgcggacg ttccagcggc
     1561 gtctcggcca atgtccctat gtggatgaag ccggcgacct gttcgccgcc ctcgacgtcg
     1621 aggatgtcca gcgcctgcgg gtcgtagctg taccagtcgg tgatccagct ggaggcgtag
     1681 cccagcgcgt tggcggcgtg ctcgaggttc atacagacgg cgccggcgga aagctgctgc
     1741 tcccattcgg ggaccttctg gctgggcaca ggggccgaga ccaccatgat ggtgaccggt
     1801 gcggcggtca gcttgcccag cacggccttc gccttgttcg gatcggtctg aacggcggcc
     1861 agcggcgtca gcgcctcggc cagtccggcg cgggtctggc ggcccagcac caccagacgc
     1921 caggggaaca gcttgccgtg atccgggctg cggacgccga gggtgaggat gtcctcgagc
     1981 tcttcggcgg atgggccggg cgcggtcaga gcctgggccg gggccgagcg gcgggtcgcc
     2041 agtctgaggc gcaattcgtc tgaagatgtc ggggcgggca gggggtcgcc gagggcgaaa
     2101 cgcggcaggg gagggcgtgt catgcggctc acatggcgtc gcccgggcgc ggcgtcaaac
     2161 ggccggcgcc gttgccggcg gcgcgcccgg tcgccatacg cacgccatga ccgattcccg
     2221 cactgccttc gacgccgacc gcccgctgcc cgtttgggac gacggaagcg accggccgcc
     2281 gccgtggagc gaccatggct ctagcatcct gttcgaaagc ccgtggatgc ggcttacgag
     2341 acacgatgcg acggcgccga cgggcctgaa ggcggactat acggtgatgc ggccgcggaa
     2401 catgtcggtg ggcgtgctgc ccgtgcacga cgacggcacg gtcaccctgg tcgggcagca
     2461 gcgcttcgcc ctgatgaact ggtcgtggga gatgccggag ggcggcgcgc cgttcgacga
     2521 ggatccgatc gagggcgcgc ggcgcgaact ggcggaggag gcggggctgg cggcggccac
     2581 ctggctgccc gcctacaagg ccgagatggc taactccatc accgacgaac gggccatggc
     2641 ctggatcgcc tgggggctga cgccggttcc ggtcgcgccc gatccgaccg agatcatccg
     2701 cgtcgtccgc gtgccgttcg gcgatctgct gcgcgagatc ggcacgggcg cgatccggga
     2761 catgttcacg ctggcgacgg cgctgagggc ctaccatatg gcgcgggaag gcgaactgcc
     2821 gcccgaactg gcgaaggcca tgctggcgag cgtatgatac acgcgaagga gccctcgatg
     2881 cccaagctgg aagtcgtcgc cgatcgccgt gaaccgtggg ccctgctgcg cgctgcggcc
     2941 gaggagacca tccgcgccga accgcatctg gcgtcgcagc tgaacgccgt gatcctgtcg
     3001 cacaacgacc tggcctcggc cctcagcttc cagatcgcgc gcaagctggg cgacggcgag
     3061 ctggggccca tgtcggtgcg cgagatctgc cgcgacgcct tcgacgccga tccgaagatc
     3121 gtcgcctcgg ccgaggccga cctgcaggcg gtggccgagc gcgatccggc catcaagacg
     3181 ctgttgcagc cgttcctcta cttcaagggc ttccaggcgc tgcaggcgca ccgggtggcg
     3241 cactggctgt gggggcaggg gcgcgaggcg ctggccttcc atttccagag ccggatttcg
     3301 gaactgttcc aggtcgacat ccacccggcc gcgcggatgg gctcgggtct gttcctcgat
     3361 catggaaccg gcatcgtcat cggcgagacg gcggtggtcg gcgacgaggt gtcga
//
LOCUS       DASVVN010000232         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_86238, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000232 DASVVN010000000
VERSION     DASVVN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86238"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF686_03645"
     CDS             <1..239
                     /locus_tag="VF686_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660937.1"
                     /translation="IVVIVRGGGGMIVMTLGVCSRMGGLIVMPVRVVPKCVGRGGGVV
                     VRTRMAVFVVRLVLVAHLGLFPSRCSAVFAAKSV"
     gene            complement(245..646)
                     /gene="acpS"
                     /locus_tag="VF686_03650"
     CDS             complement(245..646)
                     /gene="acpS"
                     /locus_tag="VF686_03650"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX8660938.1"
                     /translation="MIVGVGADLCDIRRIQRSLDRFGDRFRQRCFTELEQARSDTKPD
                     PAWSYAKRFAAKEACAKALGTGMRADVYWRDMGVVNQRSGQPTLALTGHAREHLLRLT
                     PPGHEPRIHLTLSDEHPYALAFVVIEALPVA"
     gene            complement(643..1392)
                     /locus_tag="VF686_03655"
     CDS             complement(643..1392)
                     /locus_tag="VF686_03655"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX8660939.1"
                     /translation="MVTPVRLGVNIDHVATVRNARGGVHPDPVRAAREALAAGADGIT
                     AHLREDRRHITDADIAALSDLCAVAGRPLNLEMAVTEEMLAIALRHRPHAACLVPERR
                     EEVTTEGGLAVAGHEARIRPVVKALADAGMRVSLFIEPSLEQVEAAHAVGAQVVELHT
                     GRYCHLSGDDRVVEFERLQAAATRAFDLGLEVHAGHGLDYSTAAHMLAIPEIRELNIG
                     HFLIGEAVFVGLTPAIHRMRAAMDHAGSGAT"
     gene            complement(1404..1988)
                     /gene="pyrE"
                     /locus_tag="VF686_03660"
     CDS             complement(1404..1988)
                     /gene="pyrE"
                     /locus_tag="VF686_03660"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX8660940.1"
                     /translation="MTSDDVLDEFRGAGALREGHFVLSSGLHSPVFLQKNLVFMDAER
                     CARLCKALAEKITAAVGPVDIAISPAVGGIIPGYETARWLRVPSMYVEREGGEFKLRR
                     GFHVEPGQKVVMVEDIVTTGLSSRECIAAIRAAGGDVVAAACIVDRSGGRADVGVPLV
                     ALATLDVPAYAADALPPELAALPVEDPGSRRLAK"
     gene            complement(2028..>3977)
                     /locus_tag="VF686_03665"
     CDS             complement(2028..>3977)
                     /locus_tag="VF686_03665"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX8660941.1"
                     /translation="LLHDVVEDTSATRDDIAERFGEEVASLVEGVTKLSRLELQAEHT
                     RQAENLRKFILASSKDVRVLLVKLADRLHNMRTLKHVKPEKRERISRETLEVYAPLGR
                     SIGIHSIASELEELAFEHLNPTARTAIERRLEALKLEHGRAIDGVSREVERVLSEAGV
                     KAKVFGRQKTPYSIWRKLQRKSVGFSSLSDIYGFRVLVEAEDDCYRALGVIHRAWPVV
                     PERFKDFISTPKSNNYRSLHTTVVGPSGLRIEMQIRTEAMDRVAEDGVAAHWAYKNKS
                     YGFDKEAMEKDGGRDPLQNLRHLVQVIEHGEGGEDWVEHAKLEMYLEQVFVFTPKGSL
                     ITLPRGGMPLDFAYAVHTEVGDTAVGAKINGELKPMRTQLQNGDVIEIVRGSKREIPV
                     DWRSLTVTGRARSAIRRHIRTSERGEFQKLGRATLEQTLSRAGKTLAEVSLKAMLETL
                     SVASEEDLFEAIGRGRLSAAKVGETLFPALKGRLKAGPDRKRIGDEQARLFVRGGGLT
                     PGVSVHFGHCCSPVPGDRIVGILEPEAGLTVHTIDCQRLADFADDDSVWQDLQWTPQA
                     EQGAVASARIRATIKNAPGVLGQVATIIGEAGGNILNLVMSHRQQDFFDVDIDMEVED
                     AKHATAIMAALRTNPSVDTVERARG"
BASE COUNT          575 a         1319 c         1412 g          671 t
ORIGIN      
        1 tgatcgtcgt gatcgtgcgc ggcgggggcg gcatgatcgt catgaccctc ggggtctgca
       61 gccggatggg cggcctgatc gtcatgcccg tgcgtgtcgt gcccaagtgc gtcgggcgcg
      121 gcgggggcgt ggtcgtcagg acccgcatgg ccgtgttcgt cgtgcggctt gtgctcgtcg
      181 ctcatctcgg tttgttcccc agccgctgtt ccgcggtctt tgcggcgaaa tcggtataac
      241 gtgattaagc cacgggcaag gcttcgatca ccacgaaggc gagcgcgtac gggtgctcgt
      301 cgctgagcgt cagatgtatc cgtggttcat gaccgggcgg tgtcagcctc aacaggtgct
      361 cgcgcgcatg gccggtcagg gccagggtcg gctgtcccga gcgctggttc accaccccca
      421 tgtcgcgcca gtagacgtcg gcccgcatgc ccgtacccag ggccttggcg caggcctcct
      481 tggccgcgaa ccgcttggcg tagctccagg ccggatcggg cttcgtgtcg gaccgggcct
      541 gctccagttc ggtgaagcag cgctgtctga accggtcgcc gaagcggtcg agcgagcgct
      601 ggatgcggcg aatgtcgcac aggtccgcgc cgacgccgac gatcatgttg cacccgaccc
      661 ggcgtggtcc atggcggcgc gcatgcggtg gatggcgggg gtcaggccga cgaagacggc
      721 ctcgccgatc aggaaatggc cgatgttgag ctcgcggatc tcgggaatgg ccagcatgtg
      781 ggccgcggtg gaatagtcga ggccgtggcc ggcgtggacc tcaagaccca ggtcgaaggc
      841 ccgtgtcgcc gcagcctgaa gccgttcgaa ctctacgacg cggtcgtccc ctgacaggtg
      901 gcagtagcgg ccggtgtgca gctccacgac ctgggccccg acggcgtggg cggcctcgac
      961 ctgttcgagg ctgggctcga tgaacagcga cacccgcatg cctgcgtcgg cgagggcctt
     1021 caccacgggc cggatgcgcg cctcatggcc ggcgacggcc aacccgccct cggtcgtcac
     1081 ctcctcgcgc cgctcgggca ccaggcaggc ggcgtgcgga cgatggcgca gggcgatggc
     1141 caacatctct tcggtgacgg ccatctcgag gttcagcggc cgccccgcaa ctgcgcacag
     1201 gtcgctgagg gcggcgatgt cggcgtcggt gatgtggcgg cggtcttcgc gcagatgggc
     1261 cgtgatgccg tcggcgccgg cggccagagc ctcccgcgcg gcgcgcaccg gatcgggatg
     1321 gacgccgccg cgggcgttcc gcacggtggc tacgtggtcg atattgacgc ccagcctgac
     1381 cggcgtgacc atcccgcccg ctcctatttc gccagccggc ggctgccggg atcctcgacc
     1441 ggcagggcgg cgagttcggg cggcagggcg tcggcggcgt atgcgggcac gtccagtgtg
     1501 gccagtgcga ccagcggcac gccgacgtcg gcgcggccgc ccgagcggtc gacgatacag
     1561 gcggccgcga ccacgtctcc ccccgccgcc ctgatcgccg cgatgcattc gcgcgagctc
     1621 aagcccgtgg tgacgatgtc ctcgaccatg acgaccttct ggccgggctc gacgtggaag
     1681 ccgcggcgca gcttgaattc gccgccctcg cgctcaacgt acatcgaggg gaccctgagc
     1741 cagcgggcgg tctcatagcc ggggatgatg ccgccgacgg cgggcgagat ggcgatatcc
     1801 accggaccga ccgccgccgt gatcttctcc gccagcgcct tgcacaggcg ggcgcagcgt
     1861 tcggcgtcca tgaagacgag gttcttctgc aggaagacgg ggctgtgcag tcccgaggac
     1921 agcacgaaat ggccttcgcg cagggcgccg gcgccgcgga attcgtcgag tacgtcgtct
     1981 gaggtcatgg cggctgtcat aacgagcgcc accgtcgccg caaggcccta gccccgcgcc
     2041 cgttcgaccg tatcgaccga gggattggtt cgcagcgccg ccatgatggc cgtggcgtgc
     2101 ttcgcgtcct cgacctccat gtcgatgtcg acgtcgaaga agtcctgttg ccggtggctc
     2161 atgaccaggt tcaggatgtt gccgccggcc tcgccgatga tggtggcgac ctgacccagc
     2221 acgccgggcg cgttcttgat ggtggcgcgg atgcgggcgg aggcgacggc cccctgctcc
     2281 gcctgaggcg tccattgcag gtcctgccag acggaatcgt cgtcggcgaa gtcggccaga
     2341 cgctggcagt cgatggtgtg gacggtcagc cctgcctcgg gctccagaat gccgacgatg
     2401 cggtcgccgg gcacgggcga gcaacagtgg ccgaaatgga cgctgacccc cggggtcagg
     2461 ccgccgccgc gcacgaacag acgggcctgt tcgtcgccga tgcgcttgcg gtctgggccg
     2521 gccttcagtc ggcccttgag cgccgggaac agggtctcgc cgaccttggc cgccgacagg
     2581 cgcccgcggc cgatggcctc gaacaggtct tcttccgagg cgaccgacag ggtttccagc
     2641 atcgccttca gcgagacctc ggccagggtc ttgccggcgc gggacagggt ctgctccagc
     2701 gtcgcgcggc ccagcttctg gaactcgccc cgctccgagg tgcggatgtg gcggcggatg
     2761 gcggagcggg cgcggccggt gacggtcagg gagcgccagt cgacgggaat ctcgcgcttg
     2821 gagccgcgca cgatctcgat gacgtcgccg ttctggagct gggtgcgcat cggcttcagt
     2881 tcgccgttga tcttggcgcc gacggccgta tcgccgacct cggtgtggac cgcatatgcg
     2941 aagtccagcg gcatgccgcc gcgcggcagg gtgatcagcg aacccttggg cgtgaagacg
     3001 aagacctgtt cgaggtacat ctccagcttg gcgtgttcga cccagtcctc gccgccctcg
     3061 ccatgctcga ttacctgcac gaggtggcga aggttctgca gcgggtcgcg gccgccgtcc
     3121 ttctccatgg cctccttgtc gaagccatag gacttgttct tgtaggccca gtgcgcggcc
     3181 acgccgtcct cggccacgcg atccatggcc tcggtgcgga tctgcatctc gatgcgcagg
     3241 ccagaggggc ccacaacagt cgtgtgcaag gaacggtagt tgttcgactt cggcgtcgag
     3301 atgaagtcct tgaaccgctc gggcaccacg ggccaggcgc ggtggatgac gccaagggcg
     3361 cggtagcagt cgtcctcggc ctcgaccagc acccggaagc cgtagatgtc ggacagcgac
     3421 gagaagccga ccgacttgcg ctgcagtttg cgccagatcg aatagggcgt cttctgacgg
     3481 ccgaagacct tggccttgac ccccgcttcc gacagaaccc gctcgacctc gcgcgacacg
     3541 ccgtcgatgg cgcggccgtg ctccagcttc agcgcctcca gccggcgctc gatggcggtg
     3601 cgggcggtgg ggttcaggtg ttcgaaggcc agttcttcca gctcggacgc gatcgaatgg
     3661 atgccgatcg accggcccag cggggcgtag acttccagag tctcgcggct gatgcgctcg
     3721 cgcttctcgg gtttgacgtg tttcagcgtc cgcatgttgt gcagacggtc ggccagcttc
     3781 accagcagga cgcgaacgtc cttggagctg gccaggatga atttgcgcag gttctcggcc
     3841 tgacgcgtgt gctcggcctg gagctccagc cgcgacagct tggtcacgcc ctcgaccaga
     3901 gaggccacct cctcgccgaa ccgctcggcg atgtcatcgc gggtcgccga ggtgtcctcg
     3961 accacgtcgt gcagcag
//
LOCUS       DASVVN010000233         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_33725, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000233 DASVVN010000000
VERSION     DASVVN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33725"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1381)
                     /locus_tag="VF686_03670"
     CDS             complement(128..1381)
                     /locus_tag="VF686_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEX8660942.1"
                     /translation="MSTTDRHDVIIAGAGMAGATFALAAAQGGLKVVLVDPQPFSLQL
                     APTFDGRSTAIAFSTFRMLDALGVGAALRPHACRMDRILVTDGRRPGAASRPAGSAFL
                     RFDADEIGDRTDGEPLGYMVENRRIRTALAEAVTAAGIKVRAPASVAGVEAGPGRTVV
                     TLADGVRLEAPLVVGAEGRASLVRQSAGIETVGWGYGQSGVVATVSLGRDHGNVAHEY
                     FLPAGPFAILPLTEQRASLVWTETTRRAEALKAAPDAAFQSHLMRRFGDFLEDVTVVG
                     PRFVYPLSLQLAEALTGPRAALIGDAAHAVHPVAGQGLNMGLKDAAALAEVLVEAMRL
                     GEDIGSETVLDRYARWRRFDNTALAAGFDGFVRLFSNDAPPVRLARGLGIAAVNRIPA
                     LRRAFMHEAGGATGDLPRLLRGEAV"
     gene            1518..3899
                     /locus_tag="VF686_03675"
     CDS             1518..3899
                     /locus_tag="VF686_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX8660943.1"
                     /translation="MSAALAIGQRAWFGARILWGAPIAIKFRGVLQALLAALLLAALI
                     SWNPADPSLNAASDMAPTNWLGLNGALFADLFMQSLGLAAFPAALLLIAFGLASAIGD
                     VIQQRLKPTTLKALSAAGGVLALSAALSALAPPAAWPLAAGLGGLWGDAIVGLIRMAC
                     TALRIPGAPIIAGVLFFPMALWAIGYAIGLRTSDLGDAFVWARSGRAMAQPPAKARRA
                     AVKAPPRPRPAPEPDDYASVSLDAPEETPPWEAPAYVPAPARGAPEPKVAAAKAPKAS
                     KRELDDSQAAFDFARPSGGFDLPPLGMLTKPAKRVASVDEEALKQNAKMLEGVLQEFG
                     VRGVIDQIRPGPVVTLYELVPAPGVKHGRVVALSDDIARSMSARACRISVVPGRNAIG
                     IELPNARRETVYLRDLLASTEYDKPAHLLPLALGETIGGEPYVADLARMPHLLIAGTT
                     GSGKSVGVNAMILSILYRLTPAECRFIMIDPKMLELSVYDGIPHLLAPVVTDPKKAVV
                     ALKWTVREMEDRYRRMSKLGVRNIASYNERAREAVAKGEHFERTVQTGFDEQGRPVYE
                     SEKIRPEPMPFLVVVMDEMADLMLVAGKDVEGAVQRLAQMARAAGIHLIMATQRPSVD
                     VITGTIKANFPTRISFQVTSKIDSRTILGEQGGEQLLGQGDMLYMAGGGRITRLHGPF
                     VTDQEVDEVCKHLRSQAEPDYLDLITDDPDGDGDGAFDDEGGGGSGDDLYDRAVAVVT
                     RDRKASTSYIQRRLQIGYNRAASLIERMEQEGVVSPANHAGKRDILAGPPPMG"
     gene            4217..>4551
                     /locus_tag="VF686_03680"
     CDS             4217..>4551
                     /locus_tag="VF686_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX8660944.1"
                     /translation="MTVSRRAFALGSAALAAVAALPAAAQSGLSAEDRAVVAQAQAYL
                     QNLTSARGNFVETSGAQRREGRFWIQRPGKMRFEYTNPAGLLVVSDGSNVKRYDPRLN
                     VFRQVPLGST"
BASE COUNT          681 a         1756 c         1460 g          654 t
ORIGIN      
        1 aaggcgggga cccagttctt tggatgatgt cgcatgcctg tgatccgcga tcgttgtcct
       61 gaagcgccgg cgtcaagctg gacaggactg ggtccccgcc ttcgcgggga tgcacggaac
      121 agatgggtta aaccgcctca ccccgcagca gccgcggcag atcccccgtc gccccgccgg
      181 cctcgtgcat aaaggctctc cgcagggcgg ggatccggtt cacagcggcg atgcccagtc
      241 cccgcgccag ccgcaccggc ggtgcgtcgt tcgagaacag ccgcacgaac ccgtcgaagc
      301 ccgccgccag cgccgtgttg tcgaaccgcc gccagcgggc gtagcggtcc agcaccgtct
      361 ccgagccgat gtcctcgccc agtcgcatgg cctcgaccag cacctcggcc agggccgcgg
      421 cgtccttcag ccccatgttc agcccctggc ctgccaccgg atgcacagca tgggcggcgt
      481 cgccgatcag ggcggcgcgg ggacccgtca gcgcctcggc cagctgcagc gacagcggat
      541 agacgaagcg cggcccgacc actgtcacat cctcgaggaa gtcgccgaac cttcgcatca
      601 gatgcgattg aaaggcggcg tccggcgcgg ccttcagggc ctcggcccgg cgcgtggtct
      661 ccgtccacac cagactggcc cgctgttcgg tcagcggcag gatggcgaac ggccccgcag
      721 gcaggaaata ctcatgcgcg acattgccgt ggtcccgccc gagggacacg gtggccacca
      781 ccccgctctg gccatagccc caacccacgg tctcgatccc ggcgctttgt ctcaccagtg
      841 aggcacgacc ttcggcgccc accaccagcg gcgcctccag ccggacgccg tccgccagcg
      901 tgacaaccgt ccggcccgga ccggcctcga ctccggcgac cgatgcgggg gcccggacct
      961 tgatcccggc ggccgtgacc gcctccgcca gcgccgtccg gatgcgccgg ttctcgacca
     1021 tgtagcccag cggctcgccg tcggtgcggt cgccgatctc gtcggcgtcg aagcgcagga
     1081 aggccgaacc ggcgggccgt gacgccgcgc ccggccgccg cccgtccgtg accagtatcc
     1141 ggtccatccg gcaggcgtgt gggcgcaggg ccgcgcccac gcccaaggcg tccagcatgc
     1201 ggaaggtcga gaaggcgatg gcggtcgacc gcccgtcgaa cgtcggcgcc agctgcagcg
     1261 agaacggttg cggatcgacc agcaccacct tcagcccgcc ctgcgcggcg gccagcgcga
     1321 acgtcgcccc ggccatgccg gccccggcga tgatgacgtc gtgtcggtcg gtcgtgctca
     1381 tgggccgttt gtcgcgccgg agggcgggcg cgtccagtgc cgcgaggtcg cggtttccgc
     1441 gggctggtaa acagcctctt aaccctgcgc aggcgacact ccccggtcag ttcgaccgtc
     1501 cggagttacg tcccgccatg tccgccgccc tcgccatcgg ccagcgcgcc tggttcggcg
     1561 cccgcatcct gtggggcgcg cccatcgcga tcaaattccg cggcgtgctc caagccctgc
     1621 tggcggcctt gctgctggcg gcgttgatct cgtggaatcc ggctgatccc agcctcaacg
     1681 ccgcctcaga tatggccccg accaactggc tgggcctgaa cggcgccctg ttcgccgacc
     1741 tgttcatgca gtcgctgggt ctggccgcct tcccggcggc actgctgctg atcgccttcg
     1801 gtctggcctc ggccatcggc gacgttatcc agcagcggct caagccgacg acgttgaagg
     1861 ccctttccgc tgcgggcggc gtgctcgccc tgtccgccgc cttgtcggcg ctggccccgc
     1921 ccgccgcatg gccgctggcc gccggcctcg gcggcctgtg gggcgacgcc atcgtcggcc
     1981 tgatccgcat ggcctgcacg gccctgcgca ttcccggcgc gccgatcatc gccggcgtcc
     2041 tgttcttccc gatggcgctg tgggccatcg gctacgccat cggcctgagg acctctgatc
     2101 tcggcgacgc cttcgtctgg gcccgcagcg gccgcgccat ggcccagcct ccggccaagg
     2161 cccgccgggc cgccgtcaag gcgccgcccc gtccgcgtcc ggcccccgag cccgacgact
     2221 acgcctccgt cagcctcgac gcgccggaag agaccccgcc gtgggaggcc cccgcctatg
     2281 taccggcccc cgcccgcggc gcccccgagc ccaaggtcgc cgccgccaag gcccccaagg
     2341 cctcgaagcg cgagctcgac gacagccagg cggcctttga tttcgcccgg ccctcgggcg
     2401 gcttcgacct gccgccgctc ggcatgctga ccaagcccgc caaacgcgtc gcctcggtcg
     2461 acgaggaggc gctgaagcag aacgccaaga tgctggaagg cgtgctgcag gagttcggcg
     2521 tgcgcggcgt catcgaccag atccgccccg gccccgtcgt caccctgtac gaactggtcc
     2581 ccgcacccgg cgtgaagcac ggccgcgtcg tggcgctcag cgacgacatc gcccgctcca
     2641 tgtccgcccg cgcctgccgc atcagcgtgg tccccggccg caacgccatc ggcatcgaac
     2701 tgcccaacgc ccggcgcgag accgtctatc tgcgcgacct gctggcctcg accgaatacg
     2761 acaagcccgc ccacctgctg ccgctcgccc tgggcgaaac tatcggcggc gagccctatg
     2821 tcgccgatct ggcgcgcatg ccgcacctgc tgatcgccgg caccaccggc tcgggcaagt
     2881 cggtcggggt caacgccatg atcctgtcga tcctctaccg gctgaccccg gccgagtgcc
     2941 gcttcatcat gatcgacccc aagatgctgg agctgtcggt ctatgacggc atcccgcacc
     3001 tgctggcccc ggttgtcacc gatcccaaga aggccgtcgt cgcgctgaaa tggaccgtgc
     3061 gcgagatgga ggaccgctac cgccgcatgt ccaagctcgg cgtgcgcaac atcgcctcct
     3121 acaacgagcg cgcccgcgag gccgtggcca agggcgagca tttcgaacgc accgtccaga
     3181 ccggcttcga cgagcagggc cgtccggtct acgagtccga gaagatccgt cccgaaccca
     3241 tgcccttcct cgtcgtcgtc atggacgaga tggccgacct gatgttggtc gccggcaagg
     3301 acgtggaggg cgccgtccaa cgtctggccc agatggcccg cgccgccggc attcacctga
     3361 tcatggccac ccagcgcccg tcggtcgacg tcatcaccgg caccatcaag gccaatttcc
     3421 cgacccggat cagcttccag gtcacctcca agatcgacag ccgcaccatc ctcggcgaac
     3481 agggcggcga acagctgctg ggccagggcg acatgctcta catggccggc ggcggccgca
     3541 tcacccgcct gcacgggccg ttcgtgaccg atcaggaggt cgacgaggtc tgcaaacacc
     3601 tgcgctcgca ggccgagccc gactatctcg acctgatcac cgacgatccg gacggcgacg
     3661 gcgacggggc cttcgacgac gaaggcggcg gcggttccgg tgacgacctc tacgaccgcg
     3721 ccgtggccgt ggtcacccgc gaccgcaagg cctcgaccag ctacatccag cgccgcctgc
     3781 agatcggcta caaccgcgcc gcctccctga tcgagcggat ggagcaggag ggcgtcgtca
     3841 gcccggccaa ccacgccggc aagcgcgaca tcctcgccgg cccgccgccg atgggttgag
     3901 gctcgcagcg ggcgctctcc ttcccgtgca tccccgcgaa ggcggggacc caggtttaag
     3961 cggcggcctc ggcccaaggc tggagcggct ggcagggctg ggactgcctg aacgacgctc
     4021 aaccaaacac ccgcggcggg gccaaagcct gggtccccgc cttcgcgggg atgcgcggaa
     4081 tgaaaaaccg gaaccaccgc ggatgaacgg cgcttcattc cgccgccgga atatggtcag
     4141 gctcgggcca cgcccacgcc ttttgcagtc catacgacgg catgatcgcc aaccatcccg
     4201 aacgggagac gaccctatga ctgtgtcacg ccgcgccttt gcgcttggat ccgccgccct
     4261 cgccgccgtc gcggccctgc ccgccgccgc ccagagcggc ctgtcggccg aggaccgcgc
     4321 cgtcgtggcc caggcccagg cctatctgca gaacctgacc tcggcccggg gcaatttcgt
     4381 cgagacctcg ggcgcccagc gccgcgaagg ccgcttctgg atccagcgtc ccggcaagat
     4441 gcgcttcgaa tacaccaacc cggccggcct gctcgtcgtc tcggacggct cgaacgtcaa
     4501 acgctacgac ccgcgcctga acgtcttccg tcaggtgccg ctgggctcca c
//
LOCUS       DASVVN010000234         6150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_244631,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000234 DASVVN010000000
VERSION     DASVVN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244631"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="dnaA"
                     /locus_tag="VF686_03685"
     CDS             <1..1110
                     /gene="dnaA"
                     /locus_tag="VF686_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX8660945.1"
                     /translation="QAAPAPMPMATVPPVADGARAVRAAGLQERLTFDSFVEGQGNAF
                     ALAIARQAAAWADGHFNPIFFCGPYGYGKTHLLNAIAWEAQRLRPDAKVVYLTAERFL
                     STFVRATQDRTMAAFKDSLRGADMLLIDDVQFVGGKAGTQEELLSTLTALIEDGKKIV
                     ISADRPPMALTEVEPRLRSHLASGLTCPVEPADRALKLAVARNRIQALAQLGVVSGEA
                     QAEVLEHLVDRTPGSMRELEGAVNTLAAVAGARLSSLTVDETQGLLGSALRGGPERRI
                     TVDEIQKTVADHFSLKQADLLSERRTRAVARPRQIAMWLCKQHTTRSYPDIGRRFGGR
                     DHTTVLHGVRKIEELMGLDDQIARDVETLTRKLRG"
     gene            1256..2374
                     /gene="dnaN"
                     /locus_tag="VF686_03690"
     CDS             1256..2374
                     /gene="dnaN"
                     /locus_tag="VF686_03690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8660946.1"
                     /translation="MQLTIERSALLKALGHVQSVVERRNTIPILSNVLLSAGRDKLAF
                     AATDLDMEMVDEAEAQVNVEGQITAPAHTLYEIVRKLPDGAEVSLSYNGDDPRLVVQA
                     GRSKFNLPVLPAGDFPVMSTDTTGTRFSLPKEDLARLIDKTRFAVSTEETRYYLNGLY
                     LHTVAEAGIPLLRAVATDGHRLALAETPAPEGAAGGPGVIVPRKTIDQVRRLLDDGSG
                     PVEVMVSPQKIRFEFGQASLTSKVIDGAFPDYMRVIPKGNDKQADIDNALFAQAVDRV
                     ATISAEKSRSVKLAFELDRVTLTVRNMEAGQGVEEVEIGYSEEPFEIGFNARYLLDVA
                     GQITGETAHFKFADPASPTLVLDPGDPGVQYVLMPLRV"
     gene            2400..3545
                     /gene="recF"
                     /locus_tag="VF686_03695"
     CDS             2400..3545
                     /gene="recF"
                     /locus_tag="VF686_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX8660947.1"
                     /translation="MPRAVLRRLSLTDFRSYAAATLPIAAGSVVLHGPNGAGKTNLLE
                     ALSLFTPGKGLRGATAPEMGRREPGEATGRAWAVAAVLTDGDDETKLGTGVQSAGAAR
                     RIVRIDGETAQPGRLLDHLRPVWATPEQDRLFSDARADRLKFFDRLVFAAEPSHAASV
                     SAYEKALRERLRLLTEGAEGRPADPLWLDALEIRLGEAGAAAAHARDAALTTLQSAID
                     ARADRPFPQADLGLTGEAETLAASGADEAAIAAVIRDGMVRSRARDAAAGRSLYGPHR
                     SDLTALHREKNRPAAEGSSGEQKALVLNLILAQIARLADQPAPPVLLLDEAPAHLDSA
                     RRAALFDEIEALGLQAFMTGTEAELFAPLRGRAQFVRVADGGFARED"
     gene            4342..>6150
                     /locus_tag="VF686_03700"
     CDS             4342..>6150
                     /locus_tag="VF686_03700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX8660948.1"
                     /translation="MTDQTPTARNVDTAEEAAYGADSIKVLKGLDAVRKRPGMYIGDT
                     DDGSGLHHMVYEVVDNAIDEALAGHADLVQVILNADGSVTVTDNGRGIPVDIHEGEGV
                     SAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLKLVIYRNGKRHEMKFA
                     RGDTVESLKVTGDAPMRESGERAGQPLSGTEVTFFPSVTTFAHIDFDLKTLEHRLREL
                     AFLNSGVVIKLRDDRHAEKFEEILHYEGGVEAFVRHLDKSKTPILKDVIVIRGKKEGI
                     EIDLALWWNDSYHETMLCFTNNIPQRDGGTHLSAFRASLTRVMGGYIDAAGAAKKEKV
                     SVSGEDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLCSEGLSTWFEEHPV
                     EAKAVVAKIIEAAAAREAARKARDLTRRKTSMDMASLPGKLADCQERDPAKSELFIVE
                     GDSAGGSAKQARNRENQAVLPLRGKILNVERARFDRMLSSELIGTLIMALGTGIGRDD
                     FNADKLRYHKIILMADADVDGAHIRTLLLTFFYRQMPELIERGHVYIAQPPLYKASKG
                     KSVRYLKDDAELEIYLIDEGVEGATLDLGSGERLTGK"
BASE COUNT         1014 a         2268 c         1981 g          887 t
ORIGIN      
        1 caggcggctc ccgcgcccat gcccatggcc accgtgccgc cggtcgccga cggcgcccgc
       61 gccgtgcgcg ccgccggcct gcaggagcgg ctgaccttcg acagcttcgt ggaagggcag
      121 ggcaacgcct tcgccttggc catcgcccgt caggccgcgg cctgggccga cggccatttc
      181 aacccgatct tcttctgcgg cccctatggc tacggcaaga cccacctgct gaacgccatc
      241 gcctgggagg cccagcggct gcgccccgat gcgaaggtcg tctatctgac cgccgaacgc
      301 ttcctgtcga ccttcgtgcg cgccacccag gaccgcacca tggccgcctt caaggacagc
      361 ctgcgcggcg ccgacatgct gctgatcgac gacgtccagt tcgtgggcgg caaggccggc
      421 acccaggaag aactgctgtc gacgctgacc gccctgatcg aggacggcaa gaagatcgtc
      481 atctcggccg accgcccgcc catggccctg accgaggtcg agccgcgcct gcgcagccat
      541 ctggcctcgg gcctgacctg cccggtcgag ccggccgacc gcgccctgaa actggccgtg
      601 gcccgcaacc gcatccaggc cctggcccag ctgggcgtcg tctcgggcga ggcccaggcc
      661 gaggtgctcg agcatctggt cgaccgcacc cccggctcga tgcgtgagct ggaaggcgcc
      721 gtgaacaccc tggccgccgt cgccggcgcc cgcctgtcgt ccctgaccgt cgacgagacg
      781 caggggttgc tgggttccgc cctgcgcggc ggcccggagc gccgcatcac cgtcgacgaa
      841 atccagaaga ccgtcgccga ccacttcagc ctcaaacagg ccgacctgct cagcgagcgc
      901 cgcacccgcg cggtggcccg tccgcgccag atcgccatgt ggctgtgcaa acagcacacg
      961 acccgctcct atcccgacat cggccgccgc ttcggggggc gcgaccacac cacggtcctg
     1021 cacggggtcc gcaagatcga ggagctgatg gggctggacg accagatcgc gcgggacgtc
     1081 gagacactga cgcgcaagct gcggggctga cgctgacgct ctccctcccc atcaagggga
     1141 gggagaagtc tgtggaccgc tttccagacc gttgctccgc ccggccaatc cgctagtgtc
     1201 cgtagcccta tctctttgca gcggcttgtg agtcgctgac ggcgggcgag acgacatgca
     1261 actgaccatc gaacgatccg cgcttctgaa ggccctcggc cacgttcaga gcgtcgtcga
     1321 acgccgcaac accattccga tcctgtccaa cgtcctgctc agcgccggcc gcgacaagct
     1381 ggcgttcgcc gcgacggatc tcgacatgga aatggtcgat gaggccgagg cccaggtgaa
     1441 tgtcgagggc cagatcaccg ccccggccca caccctgtac gaaatcgtcc gcaagctgcc
     1501 cgacggcgcc gaggtctcgc tcagctacaa cggcgacgac ccccgtctgg tggtccaggc
     1561 cgggcgttcg aaattcaacc tgccggtcct gccggccggc gacttcccgg tcatgtcgac
     1621 cgacaccacg ggcacgcgct tctccctgcc caaggaagat ctggcccggc tgatcgacaa
     1681 gacccgtttc gccgtctcga ccgaagagac gcgctactat ctgaacggcc tgtatctgca
     1741 tacggtggcc gaggccggca ttccgttgct gcgcgccgtc gccaccgacg gccaccgcct
     1801 ggccctggcc gagacccccg cgcccgaggg cgccgccggc ggcccgggcg tcatcgtccc
     1861 gcgcaagacc atcgatcagg tccgccgcct gctcgacgac ggctcgggcc cggtcgaggt
     1921 gatggtctcg ccgcagaaga tccgcttcga gttcggtcag gcctccctga cctccaaggt
     1981 catcgacggc gccttccccg actacatgcg cgtcatcccc aaggggaacg acaaacaggc
     2041 cgacatcgac aacgccctgt tcgcccaggc cgtcgatcgc gtcgccacca tttccgctga
     2101 aaagagccgc tcggtgaaac tggccttcga gctggaccgc gtgaccctga cggtgcgcaa
     2161 catggaggcc gggcaggggg tcgaggaggt cgagatcggc tattccgaag agcccttcga
     2221 gatcggcttc aacgcccgct atctgctgga cgtcgccggc cagatcaccg gcgagacggc
     2281 gcacttcaaa ttcgccgacc cggccagtcc aacgctggtc ctcgatccgg gcgatccggg
     2341 cgtacagtat gtgctgatgc cgctgagggt gtgaccctcc cccggttggg gaagagctga
     2401 tgccccgcgc cgtccttcgc cgcctcagcc tcaccgactt ccgctcctac gcggcggcca
     2461 ccctgcccat cgcggcgggg tcggtggtgc ttcacgggcc gaacggggcc ggcaagacca
     2521 atctgctgga ggcgctcagc ctgttcaccc ccggcaaggg gctgcgcggg gccacggcgc
     2581 ccgagatggg ccggcgcgaa ccgggcgagg cgaccggccg cgcctgggcc gtcgccgctg
     2641 tcctgaccga cggcgatgac gagacaaagc tgggcaccgg cgtccagagc gccggcgccg
     2701 cccgccgcat cgttcgcatc gacggcgaga cggcccagcc gggccgtctg ctcgaccatc
     2761 tgcgcccggt ctgggccacg cccgaacagg accggctgtt ctcggacgcc cgcgccgacc
     2821 gactgaaatt cttcgaccgg ctggtcttcg ccgccgagcc gtcgcacgcg gcctcagtct
     2881 cggcctatga gaaggccctg cgcgaacggc tgcggctgct gaccgagggg gcggaaggcc
     2941 gccccgccga cccgctgtgg ctcgacgcgc tggagatccg gctcggcgag gccggggccg
     3001 ccgccgccca cgcccgcgac gccgccctga ccaccctcca gtccgccatc gacgcccgcg
     3061 ccgatcgccc cttcccccag gccgacctcg gcctgaccgg cgaggccgaa accctggccg
     3121 cctcgggcgc cgacgaggcc gctatcgccg ccgtcatccg cgacggcatg gtccgctccc
     3181 gcgcccgcga cgccgccgcc ggccgatccc tctacggccc gcaccgctcg gacctgaccg
     3241 ccctgcaccg cgaaaagaac cgccccgccg ccgagggctc atcgggcgaa cagaaggcgc
     3301 tggtcctcaa cctgatcctc gcccagatcg cccggctggc cgatcagccc gccccgccgg
     3361 tgctgctgct cgacgaagcc ccggcccacc tcgactcggc ccgccgcgcc gccctgtttg
     3421 atgagatcga ggcgctgggc ctgcaggcct tcatgaccgg gacggaggcg gaactgttcg
     3481 cgccgctgcg gggacgggcg cagttcgtgc gggtggcgga cggcgggttc gcgagggagg
     3541 actgatccgc ccgtctcctc cctgtcgcgc agcgatgggg agggggaccg cgaagcggtg
     3601 gaggggcgcc gggtccgcgc actgcatata cccctccgtc acggcgcgaa gccgcgccgc
     3661 gccacctccc cattcgctat gcgaacaggg aggagacaat gctggcaatc cgccgccagc
     3721 catacaagtt gcaaacgcca atcccttgat ccgccacagg atcggggaag aagcagcgtc
     3781 atttcgtagc cgcatgtagg ccccgtgcga cactgttccc gtcccgtcgc aagggaggct
     3841 cgctcggccg gcgatccgaa gcggcggcgg gagcggaagg agcggggtcc gccggtttcg
     3901 gccggcggag ccgacgggcg catacacgtc cggcggtaag gaacgagggg gatactcgtt
     3961 cggcccgggg atggtcgcgc tggaccaccc gcccgccggg ggcgtttgcg gtaaggcgcc
     4021 aagaccgcca ccggacgcaa gctcccggaa aaccggccgc gcggagcgtt cgtctttcgc
     4081 cgaaacggta ttctctaccc ttatccgggc gaaggcccgg acgctccgcc cagcctcccg
     4141 taaccgccct gaatccagcg cggcgccgac gcctgcgcgc ggccttttcc agcgtcgctt
     4201 ttcctcgcaa ttccgcccgg ttttcctata tgttgcgggt atcggcgcgg atcggattcg
     4261 cgccctgaaa accgtccgtt caaggcgacc tcgccgccgc cttcagaggc cctccgagag
     4321 ggcgtttcac cgaccgaata tatgaccgat cagaccccga ccgctcgcaa cgtcgacacc
     4381 gccgaagagg ccgcctacgg cgccgattcc atcaaggtcc tcaaggggct ggacgcggtc
     4441 cgcaaacggc ccggcatgta tatcggcgac accgacgacg gctcgggcct gcaccacatg
     4501 gtctatgagg tggtcgacaa cgctatcgac gaggccctgg ccggtcacgc cgatttggtc
     4561 caggtcatcc tcaacgccga cggctcggtc accgtgaccg acaacggccg cggcatcccc
     4621 gtcgacatcc acgagggcga gggcgtctcg gcggccgagg tcatcatgac ccagctgcac
     4681 gcgggcggta agttcgacca gaattcctac aaggtctcgg gcggcctgca cggcgtcggc
     4741 gtctcggtcg tcaacgccct gtcggactgg ctcaagctgg tcatctaccg caacggcaaa
     4801 cgccacgaga tgaagttcgc ccgcggcgac acggtcgaaa gcctgaaggt caccggcgac
     4861 gcgccgatgc gcgagagcgg cgaacgcgcc ggccagcccc tgtcgggcac cgaggtcacc
     4921 ttcttcccct cggtcaccac cttcgctcac atcgacttcg acctgaagac gctggagcac
     4981 cggctgcgcg agctggcctt cctgaactcg ggcgtggtca tcaagctgcg cgacgatcgc
     5041 cacgccgaga agttcgagga aatcctgcac tacgagggcg gggtcgaggc cttcgtgcgc
     5101 catctggaca agtccaagac gccgatcctc aaggacgtca tcgtcatccg cggcaagaag
     5161 gaagggatcg agatcgatct cgctctgtgg tggaacgaca gctaccacga gaccatgctg
     5221 tgcttcacca acaacatccc ccagcgggat ggaggcaccc acctgtcggc cttccgcgcc
     5281 tcgctgacgc gcgtcatggg cggctatatc gacgccgccg gcgcggccaa gaaggagaag
     5341 gtttcggtct cgggcgagga cgcccgcgaa ggcctgacct gcgtcctgtc ggtcaaggtg
     5401 ccggacccca aattcagctc ccagaccaag gacaagctgg tctcgtcaga ggtccgcccc
     5461 gccgtcgagg gcctgtgctc ggaaggcctg tcgacctggt tcgaggagca tccggtcgag
     5521 gccaaggccg tcgtcgccaa gatcatcgag gccgccgccg cccgcgaggc cgcgcgcaag
     5581 gcccgcgacc tgacgcggcg caagacgtcg atggacatgg cctccctgcc cggcaagctg
     5641 gccgactgtc aggaacgcga cccggccaaa tccgaactgt tcatcgtcga gggcgattcc
     5701 gccggcggct cggccaaaca ggcccgcaac cgcgagaacc aggccgtcct gcccctgcgc
     5761 ggcaagatcc tgaacgtcga gcgcgcccgc ttcgaccgca tgctgtcgtc cgaactgatc
     5821 ggcaccctga tcatggcgct gggcaccggc atcggccgcg acgatttcaa cgccgacaag
     5881 ctgcgctatc acaagatcat cctgatggcc gacgccgacg tcgacggcgc ccacatccgg
     5941 accctgctgc tgaccttctt ctatcgccag atgcccgagc tgatcgagcg cgggcacgtc
     6001 tatatcgccc agccgccgct ctataaggcg tcgaagggca agtcggtccg ctacctgaag
     6061 gacgacgccg agctggagat ctacctgatc gacgagggcg tcgagggcgc gaccctggac
     6121 ctgggctcgg gcgagcggct gaccggcaag
//
LOCUS       DASVVN010000235         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_507836,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000235 DASVVN010000000
VERSION     DASVVN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507836"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VF686_03705"
     CDS             complement(<1..679)
                     /locus_tag="VF686_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEX8660949.1"
                     /translation="MSDREQQRLDFLKAAGLSDAARAPLPGDASTRRYERLTTPSGVN
                     LMLMDQPAAAESPPADPSWSPERRKAEGWNAVARLSAGRIEAFAAVAAHLTSLGLSAP
                     EIVAVDAAAGLAVIEDFGDDLFVHVIKAGEPEEPLYLAAVEALAALHDAPTPAVLTGA
                     AGDWPLLVYDVTALQGGADLFVEWLPKLDPTLAFDDAAIEAWRAAWAPVTARGAAGAT
                     VMAHRDYH"
     gene            complement(672..1142)
                     /gene="tsaE"
                     /locus_tag="VF686_03710"
     CDS             complement(672..1142)
                     /gene="tsaE"
                     /locus_tag="VF686_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX8660950.1"
                     /translation="MPDTHSLSLPDAAATARLGAAIAPRLKPGETVLLYGPLGMGKST
                     LARGLIRALTGPDEDVPSPTFTLVQFYESDPPVAHFDLYRLSRPGEAAEIGLDEALDD
                     GCAIIEWPERLGDDPARWLGPDILTVTLAEQGEGRVAALSGAGAWEMKLKDLHV"
     gene            complement(1135..1998)
                     /locus_tag="VF686_03715"
     CDS             complement(1135..1998)
                     /locus_tag="VF686_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8660951.1"
                     /translation="MTETVAFTPTRREPHVCDVLIAERAPRLTGSIAWPLVRPILYKL
                     LNYRAAVRMADAVKPLSGAGALDYMSDLLDLKVAVMNPERIPATGRCIVVANHPTGIA
                     DGIAVFDAICARRADAVFFANADALRVSPRLGEAVIPVEWVVDKRTREKTRATLTAAK
                     EAFEAERCVVMFPAGRLARVAKDGSVTDPEWAPTAASLARKYNAPIVPIHVAGPTSRL
                     FHFFDRISPELRDITLFHELLNKRRKAFQLKVGKPVPPTRLDIDAVKATYGLKAFTER
                     VLPSQPDADFA"
BASE COUNT          317 a          694 c          853 g          320 t
ORIGIN      
        1 cgtgatagtc gcggtgggcc atgacggtcg ccccggcggc gccccgcgcg gtgaccggcg
       61 cccaggccgc gcgccatgcc tcgatggcgg cgtcgtcgaa ggccagggtc gggtccagtt
      121 tcggcagcca ctcgacgaac aggtcggccc cgccctgaag cgcggtcacg tcatagacca
      181 gcagcggcca gtctccggcg gccccggtca ggaccgcggg cgtcggcgcg tcatgcaggg
      241 cggccagcgc ctcgacggcg gcgaggtaga gcggttcttc gggttccccg gctttgatga
      301 cgtggacgaa caggtcgtcg ccgaaatcct cgatcaccgc aaggccggcg gcggcgtcta
      361 cggcgacgat ctccggcgcc gagaggccca gcgacgtcag atgggcggct acggcggcga
      421 aggcctcgat acggccggcg gacaggcggg cgacggcgtt ccagccctcg gccttgcggc
      481 gttcagggga ccaagaggga tcggcgggcg ggctttccgc ggcggcaggt tgatccatca
      541 gcatcaggtt gacgccggac ggggtcgtca gccgctcgta gcggcgcgtc gaggcgtcgc
      601 cgggcaaggg cgcgcgcgcc gcgtcggaca ggccggcggc tttcagaaag tcgagacgct
      661 gctgttcgcg gtcagacatg aaggtccttc agtttcatct cccatgcgcc tgcgccggac
      721 agggccgcaa cccggccttc gccctgttcg gcgagggtga cggtcagaat atcgggaccc
      781 agccagcggg ccgggtcgtc gcccagccgc tcgggccatt cgatgatggc gcagccgtcg
      841 tccagcgcct cgtccagacc gatctcggcc gcctccccgg gacggctcag gcggtagaga
      901 tcgaaatggg ccaccggcgg atcgctctcg tagaactgca cgagggtgaa ggtcggcgag
      961 ggcacgtcct cgtcgggccc ggtcagggcg cggatcaggc cgcgcgcgag cgtcgacttg
     1021 cccatgccaa gcggcccgta cagcagcacc gtctcgccgg gcttcagccg cggcgcgatg
     1081 gccgcgccga ggcgggcggt ggcggcggcg tcgggcaggg acagggaatg cgtgtcaggc
     1141 aaaatcggcg tccggctggc tgggcaggac ccgctcggta aaggccttca ggccgtaggt
     1201 cgccttgacc gcgtcgatgt cgagccgggt cgggggcacc ggcttgccga ccttcagctg
     1261 gaaagccttg cggcgcttgt tcagcagctc gtggaacaag gtgatgtcgc gcagctcggg
     1321 cgaaatccgg tcgaagaaat ggaacaggcg cgaggtcggg ccggcgacgt ggatcggcac
     1381 gatcggcgca ttgtatttgc gcgccagcga ggcggcggtg ggggcccatt cgggatcggt
     1441 gaccgagccg tccttcgcca cccgggccag ccgtccggcc gggaacatga cgacacatcg
     1501 ttcggcctcg aacgcctcct tggcggcggt cagggtggcg cgggtctttt cgcgggtgcg
     1561 tttgtcgacg acccattcga ccgggatgac cgcctcgccc agccggggcg agacgcgcag
     1621 ggcgtcggcg ttggcgaaga agacggcgtc ggcccgccgg gcgcagatgg cgtcgaagac
     1681 ggcgatgccg tcggcgatgc cggtcggatg gttggcgacg acgatgcagc ggccggtggc
     1741 cggaatccgt tcggggttca tgaccgcgac cttcaggtcc agcaggtcgc tcatatagtc
     1801 cagggcgccc gcgcccgaga gcggcttgac cgcatcggcc atgcgtacgg cggcgcgata
     1861 gttgagcagt ttgtagagga tgggccggac cagcggccag gcgatcgagc cggtcagacg
     1921 cggcgcgcgc tcggcgatca gcacgtcgca gacgtgcggt tcccggcggg tcggcgtgaa
     1981 ggcgacggtc tcggtcattc gacgccggtt gtcgccgctc gcggcgcgca cggcaagcgg
     2041 cttggcggcg ggagcgggcg tgttaagtct ggcacacagg ttcaggggat gcggatgacg
     2101 atcaggtcgg ggcttcgggt cggcgcggcg gcgttgttgt ggatggcggg tgcggcggag
     2161 gcgatggccc aggcgccggc tcca
//
LOCUS       DASVVN010000236         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_8406, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000236 DASVVN010000000
VERSION     DASVVN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8406"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="lpxK"
                     /locus_tag="VF686_03720"
     CDS             complement(<1..684)
                     /gene="lpxK"
                     /locus_tag="VF686_03720"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX8660952.1"
                     /translation="MKLNTPRWWYARDRRHASVLRFVLKPVSWIWAAATARRIARGAP
                     VDPGAPVISIGNLTVGGSGKTPVAREVLRLLRAAGIEAHALSRGYGGKLEGPTRVDPA
                     VHTAADVGDEPLMLALDSPAWIARDRVAGARAAVSGGAKALVLDDAHQNPTLGKTLSL
                     IVVDGETRDGEWPFGDGSVFPSGPMREPLKAGLSRADAVVVLLPADLAEADPELVETF
                     GALPVFIARL"
     gene            complement(681..1967)
                     /locus_tag="VF686_03725"
     CDS             complement(681..1967)
                     /locus_tag="VF686_03725"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX8660953.1"
                     /translation="MSPALIAYRLLTRMLEPLAPRLLDARAKDGKEDPARVDERLGLA
                     SVARPAGDLVWLHGVSVGETLSLLPVVERLRKARPDLTVLVTSGTLTSATLLARRLPT
                     GVIHQFAPVDAPGAVAAFLDHWKPSLAVFVESELWPNLILAARDRGVKLVLASARITE
                     KTVQGWRRFPGAVRRILSAFDRILPQDETSAARLESLGARIDGHVNLKLAGEAPPHDP
                     AAFTRLSAAIGDRPVVVAASTHDGEEIAIVRALDKLADRLCLIIVPRHPDRSVEIAAA
                     LTRDGYGFAVRSQGRDLDRDTDLYVADTLGEMGLFLRLADVVVMGGSFSAALEKPPVG
                     GHNPLEPARLGKPAVTGPDMTNWAAVTDALVNAGGLTIVQAPWDLPAVIAPLLADDAC
                     AKQMGERGRRAAAEAGSGLDRLWDAIAPLLPPRARK"
     gene            complement(1964..2539)
                     /locus_tag="VF686_03730"
     CDS             complement(1964..2539)
                     /locus_tag="VF686_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8660954.1"
                     /translation="MTDIAFADAPMLETERLRLRPWRSGDPAAFAALNADPCVMRYFP
                     GLMATDESDALAASIQDRFRAWGFGFWAVETDALPFAGFIGLSRPAYEAPFLPAVEVG
                     WRLDPRCWGQGLATEGAHAALAFGFGQLALDQIIAVAPRANIPSIRVMERLGMTADPA
                     EDFDHPALTDHPAIRRCALHRIDHKTFEATT"
     gene            complement(2547..3233)
                     /locus_tag="VF686_03735"
     CDS             complement(2547..3233)
                     /locus_tag="VF686_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8660955.1"
                     /translation="MRPLRNPVIQAVLAWVLAQWMRFCFATIRWTRHNEGVAESVWSE
                     GGGVLCAFWHSRIALSPACWPLDRAQPAKALISLSPDGQFIAKAVGRLGFPAIRGSSS
                     NKNKADRAKGGSQALRDGLRQLKIGGLAITPDGPRGPTREMAEGLPLLAKLSKAPVLF
                     IGLSCNPTIRLRSWDRALLPLPFGRGAIVWDVDRFPEGADLTDVARDWAARLTAVEAA
                     ADALVGLERV"
     gene            complement(3230..>3720)
                     /locus_tag="VF686_03740"
     CDS             complement(3230..>3720)
                     /locus_tag="VF686_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8660956.1"
                     /translation="DDTIAANIAYARPQASMREIEAAAEAAAAADFIAALPKGYDTLV
                     GEAGLRLSGGQRQRIAIARAFLKDAPILLLDEATSALDTESEALVQAALERLMQGRAT
                     LMIAHRLSTVRNADRIHVIEAGRVVETGSHAALVKKGGLYARLAKQQSLDGEPVTVAT
                     VS"
BASE COUNT          546 a         1223 c         1464 g          487 t
ORIGIN      
        1 gagccgggcg atgaagaccg gcaaggcgcc gaaagtctcg accagttccg gatcggcttc
       61 tgcgaggtcg gcgggcagca ggacgaccac tgcgtcggcc cgggacaggc cggccttcaa
      121 cggctcgcgc atggggccag aggggaagac cgagccgtcg ccgaacggcc attcgccgtc
      181 gcgggtctcg ccgtcgacga cgatcaggct gagcgtcttt ccgagggtcg ggttctggtg
      241 ggcgtcgtcg aggacgaggg ccttcgcgcc ccccgacaca gccgcgcggg cgccggcgac
      301 gcggtcgcgg gcgatccagg cggggctgtc cagcgccagc atcaggggct cgtcgccgac
      361 gtcggcggcg gtgtgaaccg ccggatcgac gcgggtcggg ccttccagct tgccgccgta
      421 gccgcgcgac agggcgtggg cctcgatccc ggcggcgcgg agcaggcgca ggacctcgcg
      481 ggcgaccggg gtcttgcccg atccgccgac ggtaagattg ccgatggaga tgacgggcgc
      541 gccgggatcg acgggcgcgc cgcgcgcgat gcggcgggcg gtggcggcgg cccagatcca
      601 cgacaccggt ttgagcacga aacgcagcac cgaggcgtgg cgccggtcgc gggcgtacca
      661 ccagcggggc gtgttgagct tcatttgcgt gcccgcggcg gcagcagcgg cgcgatcgca
      721 tcccacagcc ggtccagccc cgatccggcc tcggcggcgg cgcggcgacc gcgttcgccc
      781 atctgtttgg cgcaggcgtc gtcggccagc aggggggcga tgacggcggg caggtcccaa
      841 ggcgcctgga cgatggtcag gccgccggcg ttgacgaggg cgtcggtgac ggcggcccag
      901 ttggtcatgt cggggccggt gaccgcgggc ttgccgaggc gggcgggctc cagcggatta
      961 tggccgccca ccgggggttt ctccagcgcg gcggagaagg aaccgcccat gaccacgaca
     1021 tccgccagcc gcagaaacag gcccatctcg ccgagggtgt cagcgacata gaggtcggtg
     1081 tcgcgatcga ggtcgcggcc ctgcgaacgg acggcgaagc cgtagccgtc gcgggtcagg
     1141 gcggcggcga tctccacgct gcggtcggga tggcgcggca cgatgatcag acacagccgg
     1201 tcggccagtt tgtccagggc ccggacgatg gcgatttcct cgccgtcgtg ggtgctggcg
     1261 gcgacgacca cgggccggtc gccgatggcg gcgctgaggc gggtgaaggc ggcgggatcg
     1321 tgcggcggcg cctcgcccgc cagcttcagg ttcacatggc cgtcgatgcg ggcgccgagg
     1381 ctttccagcc gggcggccga ggtctcgtcc tgcggcagga tgcggtcgaa ggcggacagg
     1441 atccggcgca ccgcgccggg gaaacggcgc catccctgga cggtcttctc ggtgatgcgg
     1501 gcgctggcca gcaccagttt cacgccccgg tcgcgggcgg cgaggatcag attgggccac
     1561 agctcgcttt cgacgaagac ggccagcgac ggcttccagt ggtcgaggaa ggcggcgacg
     1621 gcgccgggtg cgtcgaccgg ggcgaactgg tggatgacgc cggtcggcag gcggcgggcc
     1681 agcagggtgg ccgacgtcag ggtgcccgag gtgaccagca cggtcaggtc ggggcgcgct
     1741 tttcgcagac gctcgacgac cggcagcagg gacagggtct cgccgacgct gacaccgtgc
     1801 agccagacga ggtcgccggc ggggcgggcg accgaggcga ggccgaggcg ttcgtcgacc
     1861 cgggcggggt cttccttgcc gtccttggcg cgggcgtcca gcaggcgcgg ggccagaggc
     1921 tccagcatcc gcgtcagcag gcgataggcg atgagggccg ggctcacgtc gtcgcctcga
     1981 acgtcttgtg atcgatccgg tgcagggcgc agcggcggat ggcgggatga tcggtcaggg
     2041 ccgggtggtc gaaatcctcg gcggggtccg cggtcatgcc gaggcgctcc atcacccgga
     2101 tcgaggggat gttggcgcgc ggggccacgg cgatgatctg gtcgagggcc agctggccaa
     2161 agccgaaggc caaggcggcg tgcgcgccct cggtggccag accctgaccc cagcagcgcg
     2221 gatcgagccg ccagccgacc tcgacggcgg gcagaaaggg cgcctcatag gccgggcgcg
     2281 acaggccgat gaagccggcg aagggcaggg cgtcggtctc gaccgcccag aagccgaagc
     2341 cccaggcgcg gaagcgatct tggatactgg ccgcgagcgc gtcgctttcg tcggtcgcca
     2401 tcaggcccgg aaaataccgc atcacgcagg gatcggcgtt cagcgccgcg aaggcggcgg
     2461 ggtcgccgct gcgccaaggt cgcagacgca ggcgctccgt ttccagcatc ggagcatcgg
     2521 cgaaggcgat gtcggtcatc gccacgtcag acgcgctcca gaccgacaag ggcgtccgcg
     2581 gcggcctcga cggcggtcag ccgcgcggcc cagtcgcggg cgacatcggt caggtcggcg
     2641 ccctcgggaa agcggtcgac gtcccaaacg atggcgccgc ggccgaacgg caggggcaac
     2701 agggcgcggt cccaactcct gagccggatg gtcgggttgc aggacaggcc gatgaacagc
     2761 accggcgcct tcgacaattt ggccagcaac ggcaggccct cggccatctc ccgcgtcggg
     2821 ccgcgggggc cgtcgggggt gatcgcaagg cccccgatct tgagctggcg caggccgtcg
     2881 cgcagggcct gcgacccgcc cttggcccga tcggccttgt tcttgttgga cgacgagccg
     2941 cggatggcgg ggaagcccag ccggcccacg gccttggcga taaactggcc gtcggggctg
     3001 agcgagatca gggccttggc gggctgggcg cggtccagcg gccagcaggc tggggacagg
     3061 gcgatgcggg agtgccagaa ggcgcacagc acgccgcctc cttcggacca gacggactcg
     3121 gccacgcctt cgttgtgacg cgtccagcgg atggtggcga agcagaaccg catccactgc
     3181 gccagcaccc aggcgagaac ggcctggatg acgggattgc gcaggggcct catgagaccg
     3241 tcgcgacggt gacgggctcg ccgtccagcg actgctgttt cgcgagccgg gcgtagaggc
     3301 cgcccttctt caccagcgcc gcgtgcgagc cggtttcgac gacccggccc gcctcgatca
     3361 catggatgcg gtcggcgttg cgcacggtcg acaggcgatg ggcgatcatc aaggtggcgc
     3421 ggccctgcat cagccgttcg agggcggcct ggacaagggc ttcgctctcc gtgtcgagcg
     3481 cgctggtggc ctcgtcgagc agcaggatcg gcgcgtcctt caggaaggcg cgggcgatgg
     3541 ctatgcgctg gcgctggccg ccggacaggc gaaggccggc ctcgccgacc agcgtgtcgt
     3601 aacccttcgg caaggcggcg atgaagtcgg ccgccgccgc ggcctcggcc gccgcttcga
     3661 tctcgcgcat cgacgcctgc ggccgcgcgt aggcgatgtt ggcggcgatg gtgtcgtcga
//
LOCUS       DASVVN010000237         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_429306,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000237 DASVVN010000000
VERSION     DASVVN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429306"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF686_03745"
     CDS             <1..253
                     /locus_tag="VF686_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX8660957.1"
                     /translation="FMLLDEPFAGIDPLAIADIRQVIRYLAGEGIGVLITDHNVRETL
                     EIIDHASIISGGAVLFEGKAEDVIRDPEVRRVYLGDMYG"
     gene            complement(300..491)
                     /locus_tag="VF686_03750"
     CDS             complement(300..491)
                     /locus_tag="VF686_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660958.1"
                     /translation="MKKVLLGCAYILAITLSSTPTPTQAGGLNCWYEFIYASDENGMP
                     TDVILEVIPHCEVEQEEVE"
     gene            complement(662..1957)
                     /locus_tag="VF686_03755"
     CDS             complement(662..1957)
                     /locus_tag="VF686_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8660959.1"
                     /translation="MRLFLAALLLIAAPAAAHAEPTRAERAMMQTVTQEHDRHIALLE
                     RMVNQNSGSLNLAGVRAVGEMTRAELEPLGFEVRWVDMAETGRAGHLIATHPGRGRNI
                     LLIGHLDTVFEADNPFQRFERAGNRATGPGVGDDKGGVVVIVAALRAMQAAGTLADAN
                     ITIVLTGDEERTGAPLSISRRNLIEAGKAAAFALEFENLAVEDERDFGTIARRSSNNW
                     TLTTSGRTGHSSGVFNDTLGYGAIYEMARILDGFRRELPEPNLTYNVGVIAGGTPAAI
                     DAQGFSVTASGKTNIVASTAIARGDLRTLTAEQDARIRAGMQTIVDAHLPRTEAELVF
                     GETGYPPMAPTEGNRALLARLNAVNRDLGLPEMAEYDPARRGAADSGFVAADVDTLGG
                     LGVAGGAAHAEGEWADLDSLVRQSQRAAVLISRLSLGRD"
     gene            2111..>3026
                     /locus_tag="VF686_03760"
     CDS             2111..>3026
                     /locus_tag="VF686_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX8660960.1"
                     /translation="MLGQRLEIRQGQGLVITPQLQQAIKLLQLSNLELDAFVEAELER
                     NPLLQREDAEPAERGEAEAPAAESHDAAEMSFDEGRVADADRSLDARSDDVYGDASPG
                     ERTSDRMEDVGTGEAQVGLNDWSRAGSGGGLPDGEGREQPDTRDATLWEHLQGQASQA
                     GFSSSDHAIALTLIDGVDEGGYLRGDLNEVADRLGIPLERVEAVLTVCHGFEPTGVMA
                     RSVPECLKLQLIERNRFDPAMGCLLDNLDLLAKRDLTALKKACDVDAEDMAEMIAELK
                     ALTPRPGAGFGGEPAQTVIPDVHVRADPA"
BASE COUNT          494 a          978 c         1073 g          481 t
ORIGIN      
        1 cttcatgctg ctggacgaac ccttcgcggg catcgaccct ctggccatcg ccgacattcg
       61 tcaggtcatc cgctatctgg cgggcgaggg catcggtgtg ctgatcaccg accacaatgt
      121 ccgcgagacg ctggaaatca tcgaccacgc ctcgatcatc tcgggcggcg ccgtgctgtt
      181 cgagggcaag gccgaggacg tgatccgcga tcctgaagtg cgccgggtct atctgggcga
      241 catgtacggc tgaaccggca gggtcggaga ccttctttta aaggcctcca caggaagccc
      301 tactcgactt cttcctgttc aacttcgcaa tgcggaatga cctccagaat aacatccgta
      361 ggcataccgt tttcgtcact tgcgtagatg aactcgtacc agcagttcag accgcccgcc
      421 tgtgtaggtg taggcgtgga cgacagcgtg atcgcgagaa tgtaagcgca gcccaaaaga
      481 accttcttca ttgcaccctc cgtcatcggc ggatgacccg ccaacccgca agctgggcaa
      541 caaagcacct ccgagcaatt cacgtatgcg tgtatggcct gagatattct ttctaaggtt
      601 cagcaccgga aatcgcctga agccccgtta gcggcccgga cccctgtcct gacaaggcgg
      661 atcagtccct gccgaggctc agccggctga tcagcaccgc tgcccgctgg gactggcgca
      721 ccagactgtc cagatcggcc cattcgcctt ccgcatgggc ggctccgccg gcgacgccca
      781 gaccgccgag cgtgtcgaca tcggcggcca cgaagccgct gtcggcagcg ccgcggcggg
      841 cgggatcgta ttccgccatt tcgggcaggc cgaggtcgcg gttgacggcg ttcagacggg
      901 ccagcagggc gcgattgcct tccgtcgggg ccatgggcgg atagccggtt tcaccgaaga
      961 ccagttcggc ttcggtgcgg ggcagatggg cgtcgacgat ggtctgcatg ccggcccgga
     1021 tgcgggcgtc ctgttctgcg gtcagggtgc gcaggtcgcc gcgggcgatg gccgtcgagg
     1081 cgacgatatt ggtcttgccc gaggccgtga cgctgaagcc ttgggcgtcg atggcggcgg
     1141 gcgtgccgcc ggcgatgacg ccgacattgt aggtgaggtt cggttcgggc agctcgcggc
     1201 ggaagccgtc gaggatgcgg gccatctcgt agatggcgcc atagccgagc gtatcgttga
     1261 acacgccgct ggaatggccg gtgcggccgc tggtggtcag ggtccagttg ttggagctgc
     1321 ggcgggcgat ggtgccgaag tcccgctcat cctcgacggc aaggttctcg aactccaggg
     1381 cgaaggcggc ggccttgcca gcctcgatca gattgcggcg cgagatggag aggggggcgc
     1441 cggtccgctc ctcgtcgccg gtcaggacga tggtgatgtt ggcgtcggcc agcgtcccgg
     1501 ccgcctgcat ggcgcgcagg gccgcgacga tgacgaccac tccgcccttg tcgtcgccga
     1561 cgccggggcc ggtggcgcgg ttgcccgctc gctcgaagcg ctggaacgga ttgtcggcct
     1621 cgaacaccgt gtccaaatgg ccgatcagca ggatgttgcg gccccggccc gggtgggtgg
     1681 cgatcaggtg gcccgcgcgg ccggtctcgg ccatgtcgac ccagcggacc tcgaaaccca
     1741 gcggctccag ctcggcccgg gtcatctcgc ccacggcgcg gacgccggcg aggttcagcg
     1801 agccgctgtt ctggttcacc atccgctcca gcagggcgat gtggcggtcg tgctcctgcg
     1861 tcacggtctg catcatcgcc cgttcggccc gggtgggctc ggcgtgggcg gccgcggggg
     1921 cggcgataag cagcagggcg gcgagaaaca gacgcatggg aacctcgggg tggacgaggc
     1981 ctcagcgttt ccgatccggg gcgcagagta aaggtcgcgc ggtcgaaggc gtttagagcc
     2041 ccgttaaccg gttcggcgac acagtcgccc cagcaagttt cgggccactc gcccgaacag
     2101 ggggaatgaa gtgctcgggc agaggctgga gatacggcag gggcaggggc tggtcatcac
     2161 gccccagctg cagcaggcga tcaagctgct gcaactgtcg aacctcgagc tggacgcctt
     2221 cgtcgaggcc gagctggagc gcaatccgct gctccagcgc gaagacgccg aaccggccga
     2281 acgcggcgag gccgaggccc ctgccgccga gagccatgac gccgcggaga tgagttttga
     2341 cgagggccgt gtggccgacg ccgaccggtc gctggatgcg cgctcagacg acgtctacgg
     2401 cgacgccagc cccggcgaac gcacctccga ccggatggag gacgtcggca cgggcgaggc
     2461 ccaggtcggg ctgaacgact ggtcgcgcgc cgggtcgggc ggcggcctgc ccgacggcga
     2521 gggccgggaa cagcccgaca cgcgcgacgc caccctgtgg gagcatctgc agggccaggc
     2581 atcgcaggcg ggcttctcct cttcagacca cgccatcgcc ctgaccctga tcgacggggt
     2641 cgacgagggc ggctatctgc gcggcgacct gaacgaggtc gcggaccggc tgggcattcc
     2701 gctggagcgc gtcgaggcgg tgcttaccgt ctgccacggc ttcgaaccga ccggcgtcat
     2761 ggcgcgctcg gtccccgaat gcctgaagct gcagctgatc gagcgcaacc ggttcgaccc
     2821 ggccatgggc tgtctgctgg acaacctcga cctgctggcc aaacgcgacc tgacggccct
     2881 gaagaaggca tgcgacgtcg acgccgagga catggccgag atgatcgccg agctgaaggc
     2941 gctgacgcca cggccgggcg cgggcttcgg cggcgagccg gcccagacgg tgatcccgga
     3001 cgtgcatgtg cgggccgatc cggccg
//
LOCUS       DASVVN010000238         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_192291,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000238 DASVVN010000000
VERSION     DASVVN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192291"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VF686_03765"
     CDS             complement(<1..654)
                     /locus_tag="VF686_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX8660961.1"
                     /translation="MKLRTIGLAGALVLALLGPGCAPSQPPAPRPAQTLDMGRPRNPV
                     FAAADPHLAVAEGRWWVYATTPQEAGRPDWDRFYAWSSPDLRRWSRSGPIFSFDGVDW
                     IDDDGRPDHGLWAPAVAQANGRYYLYYSVGPQNPTPSRLGVAVGYGPTGPFTDSGRPL
                     YTGGDGFEAIDPMVFVDPRTGTAYLYAGGSAGSTLKVFELEPSMTAIRREVAIAQPPN
                     "
     gene            733..>1518
                     /locus_tag="VF686_03770"
     CDS             733..>1518
                     /locus_tag="VF686_03770"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX8660962.1"
                     /translation="MSIKTLAAALTLVAVLPAPAFAADVTRAPFGRTPQGEAVEAITL
                     TNGRVTARVITLGASLQSLTAPDRQGRSADVVLGYPTLEGYIAKPEYFGATVGRVANR
                     IARGQFTLDGRTYQTPVNNGPNALHGGTKGFDKVVWRVVGTERGPTASVTLAYDSPHM
                     DQGYPGELRVTATYSLNARNELTVDYRAITDRPTVVNISNHAYWNLGGEGSAAGVMGH
                     RLTIPADSFTPVDENLIPTGEFRPVAGTVFDFRRPMVIGERVRD"
BASE COUNT          235 a          547 c          530 g          206 t
ORIGIN      
        1 gttcggcggc tgcgcgatgg cgacctcgcg ccggatcgcc gtcatcgagg gctccagctc
       61 gaacaccttc agcgtgctgc ccgcgctgcc gccggcgtag aggtaggccg tcccggtgcg
      121 cggatcgacg aacaccatcg ggtcgatggc ctcgaagccg tcgccgccgg tgtagagcgg
      181 ccgcccgctg tcggtgaacg gaccggtggg gccgtagccc acggcgacgc cgagccggct
      241 gggcgtcggg ttctgcggac cgaccgaata gtagaggtag tagcggccgt tcgcctgggc
      301 gacagcaggc gcccacagcc catggtccgg ccggccgtca tcgtcgatcc agtcgacgcc
      361 gtcgaagctg aagatcgggc cgctgcgcga ccaccgccgc aggtcgggcg atgaccaggc
      421 gtagaaccgg tcccagtcgg gccggccggc ctcctgcggc gtggtggcgt agacccacca
      481 gcggccctca gccaccgcga gatgcgggtc ggcggccgcg aagaccggat tgcgcggccg
      541 ccccatgtcg agggtctggg cgggccgcgg cgccggaggc tgcgaaggtg cgcagccggg
      601 tcccagcagc gccaggacca gcgcgcccgc aaggccgatc gttcgcagct tcatccgccc
      661 tcccttgcat ttatcagaca atatcgctct atcgcgggca agcggacctt agatccgacc
      721 gaggaggata ccttgagcat caagacgttg gcggccgccc tgacgctggt cgccgtcctg
      781 ccggcgcccg cctttgccgc ggacgtcacc cgcgccccct tcggacgcac gccgcagggc
      841 gaggccgtgg aggccataac cctcaccaac ggccgcgtca cggcgcgcgt gatcacgctg
      901 ggcgcctcgc tgcagtcgct gaccgcgccc gaccgccagg gacgcagcgc cgacgtggtg
      961 ctcggctatc ccaccctcga gggctacatc gccaagccgg agtacttcgg cgccacggtt
     1021 ggtcgtgtgg ccaaccgcat cgcgcgcggc caattcacgc tcgacggccg cacctaccag
     1081 acgcccgtca acaacggccc taatgcgctg cacggcggca cgaagggctt cgacaaggtg
     1141 gtctggcgtg tggtcgggac cgagcgcggg ccgacggcga gcgtgacgct ggcctatgac
     1201 agcccgcaca tggaccaggg ctaccccggc gagcttcgcg tcacggccac ctattcgctg
     1261 aacgcccgca acgagctcac cgtggactac cgggcgatca ccgaccggcc gacggtggtg
     1321 aacatctcca accacgccta ctggaacctg ggcggggagg ggtccgccgc gggcgtgatg
     1381 ggccaccggc tgacgatccc ggcggacagc ttcacgccgg tcgacgagaa cctgatcccg
     1441 acgggcgaat tccgccccgt ggcgggcacc gtatttgact tccgccggcc gatggtgatc
     1501 ggcgagcgcg tgcgcgac
//
LOCUS       DASVVN010000239         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_140702,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000239 DASVVN010000000
VERSION     DASVVN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140702"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..467
                     /locus_tag="VF686_03775"
     CDS             201..467
                     /locus_tag="VF686_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660963.1"
                     /translation="MRITALIAASAAALALAACQPAAEDKAADASADAAAAADTATDA
                     AATADAAAADATAAAATATDAAAAPAADAAMAPAAPAAEEKAAH"
     gene            669..1061
                     /locus_tag="VF686_03780"
     CDS             669..1061
                     /locus_tag="VF686_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660964.1"
                     /translation="MRTAALIVAAAAAVTLSACNRPAEDATDATTEAAEASGAAADTA
                     AMSADGSTGGSAATGNTGGSGSGSTSGSGSASGSRSTSGPGGQTMAPSSGSTVSSGAT
                     APNAQPAAAGDNTTDTARRLNPPQNNQQ"
     gene            1151..>1725
                     /gene="mnmD"
                     /locus_tag="VF686_03785"
     CDS             1151..>1725
                     /gene="mnmD"
                     /locus_tag="VF686_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEX8660965.1"
                     /translation="MAEDRSPRLIWTEDGAPRSGRFDDVYFSREDGLAETQAVFLAGC
                     GLPGGWAGRRRFTVAELGFGTGLNIAALLDLWRREGPPGGRLHIFSVEGFPLDREEAA
                     RALSAWPELADAAEALLAVWPEPTPGFHRLDLPAFNAVLDLAVGEVDRALAQWTGRAD
                     AWFLDGFAPSTNPAMWSDAVMDGIAARSAPGA"
BASE COUNT          238 a          635 c          592 g          260 t
ORIGIN      
        1 cgacctcgcc gaccaccacg cctccggcgc agtagtcgac aacacggccg gaagggcggg
       61 cgcatcgtcc ttccggtcgg ttgcctcaca ctggttcacg aatgcgtgag aatttagctt
      121 gcgcttctcc tcacgatccc gttatctcgg gcattgcgag gattcatgac ctcgtgtgac
      181 ccttctgaag gaagaaaacc atgcgcatca ccgctctgat cgccgcttcg gccgccgctc
      241 tggccctggc tgcttgccaa cccgccgccg aagacaaggc cgctgacgct tcggccgacg
      301 ccgctgccgc cgccgacacc gccacggacg ccgctgcgac cgccgacgcc gccgccgccg
      361 acgccactgc cgccgccgcg accgccacgg acgccgctgc tgctccggcc gccgacgccg
      421 ccatggcccc ggctgccccg gctgctgaag aaaaagccgc tcactaagag cgacatcgac
      481 acgggccctc gggccttgtt gaacgaaagg gtcgtcctca cgggcggccc ttttttcttg
      541 cgcctgctcc ccggtcttgc gggttgcggg aacatcggtc gcccgacggg atttcaccct
      601 gtgcaacaag cttggcggtc ggcatcctga tggtcgggat ccgccgccgc agccacgagg
      661 atgaaccgat gagaaccgcc gccctgatcg tcgccgccgc cgcggccgtc accctgtccg
      721 cctgcaaccg gcccgccgag gatgcgaccg acgccaccac cgaagccgcc gaagcttcgg
      781 gcgccgccgc cgacacggcc gccatgagcg ccgatggttc gaccggcgga agcgccgcga
      841 ccggaaacac gggtggctcc ggctcgggtt cgacttcggg ttctggctcg gcttcgggct
      901 cgcgctcgac gtctggaccc ggcggccaga ccatggcgcc gtcgtccggc tcgacggtgt
      961 ccagcggcgc cacggctccg aacgcccagc cggccgccgc gggcgacaac accaccgaca
     1021 ccgcgcgccg cctgaacccg ccgcagaaca accagcaata gacgcctgca tctctctgaa
     1081 agggccgccc tgatgatcgg ggcggtcctt ttttcttgcc cggagccgcg acgcgtatag
     1141 gccgggggtc atggccgaag accgctcgcc ccgcctgatc tggaccgaag acggcgcgcc
     1201 gcgttcgggc cggttcgacg acgtctattt ctcgcgcgag gacgggctgg ccgagaccca
     1261 agcggtgttc ctggccggct gcggcctgcc cggcggctgg gcggggcgtc ggcgcttcac
     1321 cgtggccgag ctgggctttg gcacggggct caacatcgcc gccctgctgg acctgtggcg
     1381 gcgggagggg ccgccgggcg ggcggctaca cattttttcg gtcgagggtt tcccgctgga
     1441 tcgcgaggag gcggcgcgcg cgctgtcggc ctggcccgaa ctggcggatg cggcggaagc
     1501 cctgctggcc gtctggccgg agccgacgcc gggattccac cggctggatc tgccggcgtt
     1561 caacgccgtg ctggatctgg ccgtcggcga ggtcgaccgc gcgctggcgc aatggacggg
     1621 gcgggccgac gcctggttcc tcgacgggtt cgcgccctcg accaatccgg cgatgtggtc
     1681 cgacgcggtg atggacggga tcgcggcgcg gtcggcgccg ggggc
//
LOCUS       DASVVN010000240         4370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_351228,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000240 DASVVN010000000
VERSION     DASVVN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351228"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..828)
                     /locus_tag="VF686_03790"
     CDS             complement(112..828)
                     /locus_tag="VF686_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX8660966.1"
                     /translation="MTSAVGRARRLLMRPSGWIEAAPDGYALRVGRDRRARVLLTVDE
                     AAFRQLTGNPGLKIRPGGGWMARVAPAASPVSQEPGRPGVIEGVRAVMEEDGALTSRR
                     ANVGQSAIVWLARRRDVDGRAWLAPREIAAADRLTRDAEAALRGPSVTMRWDALPRSG
                     AGGDAASRTGPGASAMAAGARVETALTACGPSRGMVEAICIRASALQAAERDLGLGRR
                     CGKALLKTGLAALADHYRIG"
     gene            complement(825..1181)
                     /locus_tag="VF686_03795"
     CDS             complement(825..1181)
                     /locus_tag="VF686_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEX8660967.1"
                     /translation="MSGTYRVPVTEEDRIRAALAIQLVAAATGVAAERMRAQIRLGGV
                     ECRARRLAMYLAYVTFGWPLERVAHAFGLNRATAAAACRWAEDERDRPTLDAMLDRLE
                     RCVREVLDAPLCEVPA"
     gene            1481..2194
                     /locus_tag="VF686_03800"
     CDS             1481..2194
                     /locus_tag="VF686_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8660968.1"
                     /translation="MRVLLIEDDHATAQSIELMLKSEGFNVYTTDLGEEGIDLGKIYD
                     YDLILLDLNLPDMNGLEVLRQLRVGKVNTPVMILSGTSEIETKVKTFGGGADDYMTKP
                     FHKDELIARTHAVVRRSKGHAQAIIHTGEIAVNLDGKTVEVHGHRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLATAAGGKHYIETVWGRG
                     YVLRDPSETSAPAPVSAAA"
     gene            complement(2247..3170)
                     /locus_tag="VF686_03805"
     CDS             complement(2247..3170)
                     /locus_tag="VF686_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8660969.1"
                     /translation="MAAHRLPGPLQARIEAMAGDLMRADGISVDFSAPAGAPALAAPD
                     SVSWRVFKNPIALFIGGVAAVLLELAEPKVRAGVWDHTSFRTEPVTRLKRTGLAAMVT
                     VYAPRETAEAMIAGVGRRHARVRGVTEGGAAYAADDPDLLDWVQATASYGFLHGYHRF
                     VEPLPAADRDRFYAEAAPAARLYGALNSPTNETELEQAFAAMTPRLEPSPVIREFLDI
                     VRRTPALPSAMRLLQPMLVRAAVDILPDWARERLELGAADGLAVWERPIVRALGRAAD
                     RFVLETAPPAQACVRMGLPADWLYRPRAEAA"
     gene            complement(3251..3652)
                     /locus_tag="VF686_03810"
     CDS             complement(3251..3652)
                     /locus_tag="VF686_03810"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8660970.1"
                     /translation="MKVLPLALAALALTGGAAAAETVIITLTGVQARPGPIKASLNTR
                     EQFLRAAPAYEAVAEPAAGGVTLTFRNVAPGDYALMVMHDLNGNDRFDYGTDGWAFSN
                     SSLPMMGPPVFDERKFTVATAPVTLTETMQY"
     gene            complement(3777..>4370)
                     /locus_tag="VF686_03815"
     CDS             complement(3777..>4370)
                     /locus_tag="VF686_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX8660971.1"
                     /translation="QVPRPNPATAFQMEGPFYPYDDMPTDQDADLVRVTGVDAQAVGE
                     VTHLTGTVWDRFGGPLAGALLEVWQCDQTGRYIHRDDPNTDRARDAGFQGYGKVVADS
                     RGGFRFRTIKPVSYAAIIGGAEIWRAPHIHLTVSTRGIRRLTTQLYVAGAQQNEGDIV
                     LGPLTSAERAGLIRPFMPGSGAESGALTAHYDIVVAI"
BASE COUNT          730 a         1491 c         1479 g          670 t
ORIGIN      
        1 gcatgtgtga gacgccgcgt cgcccgcgcg gccgaaggat tacctttagg gccgaagcct
       61 tcggggcaat agttacgagc ctgtaacgtc cggcggcgcc cgctccgtcg gtcaaccaat
      121 gcggtagtgg tcggccaggg cggccaggcc ggttttcagc aaggccttgc cgcaccggcg
      181 acccaggccg aggtcccgtt ccgccgcctg cagggccgac gcccggatgc atatcgcctc
      241 gaccatcccg cgcgacggtc cgcaggccgt tagcgccgtc tccacgcggg cgcccgccgc
      301 catcgccgat gcgccgggac ctgtccggct ggccgcgtcg ccgcctgcgc ccgaacgcgg
      361 cagggcgtcc catcgcatgg tgacggacgg cccgcgcaac gcagcctcag cgtcccgggt
      421 caggcgatcg gccgcggcga tctcgcgggg agccagccag gcgcggccgt ccacatccct
      481 gcgccgcgcc agccagacga tggccgactg accgacgttg gcgcgccgcg acgtgagcgc
      541 gccgtcctcc tccataaccg cccggacacc ttcgatcaca cccggccggc ccggctcctg
      601 cgagacgggc gacgctgcgg gggcgacgcg cgccatccag cctccgcccg ggcggatttt
      661 cagtcccggg tttcccgtca gttgacggaa cgcggcctcg tcgaccgtca gaaggacccg
      721 cgcacgccgg tcccggccga ccctcagggc atagccgtcc ggcgcggcct cgatccagcc
      781 actgggccgc atcaggaggc gacgggcccg ccctacggca ctcgtcatgc cggcacctcg
      841 caaaggggcg cgtccagcac ctcgcgcacg cagcgctcca gccggtccag catggcgtcg
      901 agcgtcgggc ggtcacgttc gtcctccgcc caacggcagg ctgccgccgc ggtggcgcga
      961 ttcagcccga aggcgtgggc gacccgctcc agcggccagc cgaaggtgac ataggccaga
     1021 tacatcgcca gccggcgggc gcggcattcg acgccgccca gccggatctg cgcccgcatc
     1081 cgctccgccg ccacccccgt cgccgccgcc accagctgga tcgccagcgc ggcgcgaatg
     1141 cggtcctcct ccgtcaccgg cacgcgatac gtcccgctca tcgactgtcc tcccatcatc
     1201 attgaccgac accgtccaac cggcggcgtc ccgttgagat aggaatataa tcctgccata
     1261 cgtcgatttc ggacgtatgg tcgggcgggg ccgatccgcc gaaaagaaag tgcgttcggt
     1321 tgactcggga ctctcgacgc cgattcgcaa atcagcctac gattcgcctc agtggggtgc
     1381 tgttaacctt tcttaagttt ttactcggtt aactcccgaa caaggttacc cggcatcggg
     1441 tgtcgggaca tactgtcaag ctttgcctgg aggggcatca atgcgcgttc tgcttatcga
     1501 agacgatcac gcgaccgcgc agagcatcga gttgatgctg aagtcggaag gcttcaacgt
     1561 ctacacgacg gacctcggcg aagagggcat cgatctcggc aagatctatg actacgacct
     1621 gatcctgctc gacctcaacc tgccggacat gaacggcctt gaggttctgc gccagcttcg
     1681 cgtcggcaag gtcaacactc cggtgatgat cctctcgggc acctcggaga tcgagaccaa
     1741 ggtcaagacc ttcggcggcg gcgccgacga ctacatgacc aagcccttcc acaaggatga
     1801 gctgatcgcg cgcacccacg ccgtggtccg ccgctcgaag gggcatgccc aggccatcat
     1861 ccacaccggc gagatcgcgg tgaacctgga cggcaagacg gtcgaggtgc acggccaccg
     1921 tgtccacctg acgggcaagg aataccagat gctggagctc ctctcgctcc gcaagggcac
     1981 caccctgacc aaggaaatgt tcctgaacca cctgtacggc ggcatggacg agcccgagct
     2041 gaaaatcatc gacgtcttca tctgcaagct gcgcaagaag ctggcgacgg ccgcgggcgg
     2101 caaacactac atcgagaccg tctggggccg cggctatgtg ctgcgcgacc cgtcggaaac
     2161 ctcggctccg gctccggtca gcgccgcggc ctgatcgacc gacaacccga tgtgaaagcc
     2221 cgtcggatcg ctccggcggg ctttttctat gcggcctcgg cgcgcgggcg gtagagccag
     2281 tccgccggca gacccattcg aacacaggcc tgggctggcg gcgcggtttc cagcacgaag
     2341 cgatcggcgg cgcggccgag tgcccggacg atcggccgct cccagacggc cagaccgtcg
     2401 gccgcgccca gctccagccg ttcacgcgcc cagtcgggca ggatgtccac cgccgcccgg
     2461 accagcatcg gctgcaacag acgcatggcg gagggcaggg cgggggtccg gcggacgatg
     2521 tcgagaaact cgcggattac cggcgagggc tcaagacggg gcgtcatggc ggcgaaggcc
     2581 tgttccagct ccgtctcgtt cgtcgggctg ttcagggcgc cgtacagccg ggccgcggga
     2641 gccgcctctg catagaaccg gtcgcgatcg gccgcgggca ggggttcgac gaagcgatgg
     2701 tagccatgca ggaagccgta ggaggcggtg gcctggaccc agtccagcag atccggatcg
     2761 tcggccgcat aggcggcgcc gccttcggtc acgccgcgga ctcgcgcatg gcggcggccg
     2821 acgccggcga tcatcgcctc ggccgtctcg cgcggcgcat agacggtgac catggcggcc
     2881 aggccggtgc gcttcagccg cgtgaccggc tcggtccgga agctggtgtg gtcccagacc
     2941 cccgcgcgca cctttggctc ggccagctca agcaggacgg ccgccacgcc gccgatgaac
     3001 agggcgatcg gattcttgaa cacgcgccat gagacggagt ccggagcggc gagtgcgggc
     3061 gctccggcag gcgccgagaa gtccacgctg atcccgtcgg cccgcatcag atcgccggcc
     3121 atcgcctcaa tgcgagcctg cagcgggccg ggaagtcggt gggcagccat gcgaggataa
     3181 ggccaagcta gccgcggcag ttccaccggt gcgacaacgc cgccggattt ctccggcggg
     3241 cgcgccacgc ttaatactgc atcgtttcgg ttagcgtcac cggagcggtc gcgacggtga
     3301 atttgcgctc gtcgaagacg ggcgggccca tcatcggcag gctgctgttc gagaaggccc
     3361 agccgtcagt cccgtagtcg aaccggtcgt tgccgttcag gtcgtgcatg accatcagcg
     3421 catagtcgcc gggagccacg ttccggaacg taagcgtcac gccgccggcg gcgggttcgg
     3481 cgacggcctc ataggcgggg gcggcgcgca ggaattgctc gcgggtgttc aggctggcct
     3541 tgatcgggcc ggggcgggcc tggacgccgg tcagggtgat gatgacggtc tccgccgcgg
     3601 cggctccgcc agtcagggcc aaggcggcga gggcgagtgg gaggactttc acgggtttcg
     3661 ttccttcggt tatgaagaac tttataacgc aaagtgtatt agcgtgtcaa tcttcagaga
     3721 gggcgcgcgc agggcggtga gacggatcgg gtgtgggacc cgcaacgcct tcggtattaa
     3781 atcgccacca cgatatcgta gtgggcagtg agcgcgccgc tttcggcgcc ggatccgggc
     3841 atgaaggggc ggataagccc cgcgcgctcg gccgatgtga gcggtcccag cacgatgtcg
     3901 ccttcgttct gctgcgcgcc cgcaacgtat agctgggtcg tcaggcggcg gatgccgcgc
     3961 gtcgagacgg tcaagtgaat gtgcggcgcg cgccagatct ccgcaccgcc aatgatggcg
     4021 gcatacgaga ccggcttgat ggtgcggaac cggaagcctc cgcgggagtc ggcgaccacc
     4081 ttgccatagc cctggaagcc ggcgtcgcgg gccctgtccg tattaggatc gtcccggtgg
     4141 atgtaacgcc cggtctgatc gcactgccaa acctcgagga gagctccggc aagcggtccg
     4201 ccgaaccgat cccaaacggt tccggtcaaa tgggtgacct cgcctacggc ctgagcgtcc
     4261 acgcctgtga cgcgcaccag atcggcgtcc tggtcggtcg gcatatcgtc gtagggatag
     4321 aaggggcctt ccatctggaa cgccgtggcg gggttcgggc gcggcacctg
//
LOCUS       DASVVN010000241         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_456095,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000241 DASVVN010000000
VERSION     DASVVN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456095"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..>1677
                     /locus_tag="VF686_03820"
     CDS             191..>1677
                     /locus_tag="VF686_03820"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX8660972.1"
                     /translation="MTRRLRSRAWFDNPDNPDMTALYLERYLNYGLTLEELRSGKPII
                     GIAQTGSDLSPCNRHHLVLAERVREGIREAGGIALEFPVHPIQETGKRPTAGLDRNLA
                     YMGLVELLHGYPIDGVVLTIGCDKTTPACLMAAATVDIPAIALSVGPMLNGWHRGERV
                     GSGSIIWKAREMMAAGEIDYEGFIEMVARSAPSVGYCNTMGTATTMNSLAEALGMSLP
                     GSAAIPAPYRERPQVAYLTGKRIVDMVHEDLKPSKILTRQAFENAIVVNSAIGGSTNA
                     PVHLTALARHAHIDLPLKDWETIGHQVPLLVNLQPAGAYLGEDFHQAGGVPAVVAELM
                     RHGLIHEDALTVNGRTIGENCRDAEAVLPDVIRDFDTALVEDAGFLILNGNVFSSAVM
                     KTSVISAEFRARYLSDPSSPDAFEGVAVVFDGSEDYHRRIDDPAENITADSILVMRGA
                     GPVGHPGAAEVVNMRPPAYLIRQDILALPCIGDGRQSGTSGSPSIL"
BASE COUNT          284 a          602 c          526 g          265 t
ORIGIN      
        1 caacggtccc cgtaacgcga aagcaggggg ccacatcacg gttccgggct aaccgcccgc
       61 gctccgcctc ctccccgcac tttccggcag cgatgcccga aagcgcaaac ccatgcctgc
      121 cgacggcgcc attgccaagg ctcgggcgca gcgtctattt gtcggagtat tgacctccac
      181 gagctgttga atgacccgcc gactccgttc ccgcgcctgg ttcgacaacc ccgataatcc
      241 ggacatgacg gcgctctatc tggagcggta cctgaactat ggcctcacgc tggaggagct
      301 gcgctcgggc aaaccgatca tcggcatcgc gcagacgggc tcggacctca gcccctgtaa
      361 ccgccaccat ctggtgctgg ctgaacgggt gcgcgagggc attcgcgagg ccggcggcat
      421 cgccctggaa ttcccggtcc atccgatcca ggaaaccggc aaacgcccga ccgccggcct
      481 ggaccgcaac ctggcctata tgggtctggt ggaactgctg cacggctatc cgatcgacgg
      541 cgtggtcctg accatcggct gcgacaagac cacgccggcc tgcctgatgg cggccgcgac
      601 ggtggacatt cccgccatcg ccctctcggt cggtccgatg ctgaacggct ggcaccgggg
      661 cgagcgggtc ggctccggct cgatcatctg gaaagcccgc gagatgatgg ccgctggcga
      721 gatcgactac gagggcttta tcgagatggt cgcccgctcg gcgccctcgg tcggctactg
      781 caacaccatg ggcacggcga ccacgatgaa cagtctggcc gaggcgctgg gcatgtcgct
      841 gcccggctct gccgccattc cggccccgta ccgcgaacgc ccgcaagtcg cctatctgac
      901 cggcaaacgc atcgtcgaca tggtccacga ggacctgaag ccgtcgaaga tcctgacccg
      961 ccaggcgttc gagaacgcca tcgtcgtcaa ctccgccatc ggcggttcga ccaacgcccc
     1021 ggtccacctg accgccctgg cgcggcacgc ccacatcgac ctgccgctga aggattggga
     1081 gacgatcggc catcaggtgc cgctgctggt caatctgcag ccggcgggcg cctatctggg
     1141 ggaggacttc catcaggccg gcggcgtgcc cgccgtcgtg gccgaactga tgcggcacgg
     1201 cctgatccac gaggacgccc tgaccgtgaa cggccggacc atcggcgaga attgccggga
     1261 cgccgaggcc gttctgcccg atgtgatccg cgacttcgac acggccctgg tagaggacgc
     1321 cggcttcctg atcctgaacg gcaacgtctt cagcagcgcg gtcatgaaga ccagcgtcat
     1381 cagcgccgaa ttccgcgccc gctatctgtc ggacccctcc agccccgacg ccttcgaggg
     1441 cgtggcggtc gtgttcgacg ggtcggagga ctatcaccgc cgcatcgacg atccggccga
     1501 gaacatcacc gcggacagca ttctggtcat gcgcggagca ggccccgtcg gccacccggg
     1561 cgcggccgag gtcgtcaaca tgcggccgcc cgcctatctg atccgtcagg acatactggc
     1621 gctgccctgc atcggcgacg ggcgccagtc gggcacctcc ggttcgccct cgatcct
//
LOCUS       DASVVN010000242         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_8629, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000242 DASVVN010000000
VERSION     DASVVN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8629"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VF686_03825"
     CDS             complement(<1..1278)
                     /locus_tag="VF686_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEX8660973.1"
                     /translation="MIRSLNRRHALALMGGTAAAVSLPAFAQYEDWAALGADVKAEFK
                     WAWDHYVARAWGKDEINPVSGTSQSFFIEGHDLGLSLVEALDTLWIMELDEEFQAGVD
                     WVKTHLDFDIDGFAQVFETNIRLVGGLLSAHLASGDPVLLSKARDLADRLLKAFDASP
                     HGLPYRYVNLRTGAVRDPMTNLAEIGTYATEFGLLGQLTGEPKYYAAAKRAMKHALDM
                     RSDKGLMAANIHAETGQFTSRNASIDVYADSFYEYLWDSWEMFGDADMKRWAEECTGA
                     MIAHQGRRYEGRLWFPMVDYETAEVTGTSQTVLGGYLAGLLGQVGRKAEGDDFLASYT
                     DMQERYGIIPESIEVTTGTARGKRTGLRPEFADACLNLWLGDRDERYRALTAVHYRAM
                     KETSKAAYGYTSLADITTRPMTQGDNCPGYWWSE"
     gene            complement(1489..>2254)
                     /locus_tag="VF686_03830"
     CDS             complement(1489..>2254)
                     /locus_tag="VF686_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX8660974.1"
                     /translation="RMTYCVGMLVEEGLAMIADTRTNAGVDSISTYRKLHVVDRPGER
                     VLGICTAGNLSVTQTALAMAREGVHLNVGDEPETLETAPTLFRCAQVLGHALRSVRLS
                     IAPALEADSLNTSASMLLGGQIGGGQMGLFLIYSQGNFIECGRDTPYLQIGEAKYGKP
                     ILESGLAFDSPLAEAVKLGLISFSTTMRSNVGVGAPFDLITIRRGFLSGDQRRIEEDD
                     PYFADLHERWTRAQTTARRSMPEPPWMNGPRLAVSS"
BASE COUNT          360 a          789 c          721 g          384 t
ORIGIN      
        1 ctcggaccac cagtagcccg ggcagttgtc gccctgggtc atcggccggg tcgtgatatc
       61 cgccagcgac gtatagccgt acgccgcctt cgaggtctcc ttcatcgccc ggtagtgcac
      121 cgccgtcagc gcccggtacc gctcgtcgcg gtcgccgagc cacaggttca ggcaggcgtc
      181 ggcgaactcg gggcgcaggc cggtgcgctt gccgcgcgcc gtccccgtcg tgacctcgat
      241 cgattccggg atgatgccgt agcgctcctg catatctgtg tagctggcca ggaagtcgtc
      301 gccctcggcc ttgcgcccca cctgccccag cagcccggcc agatagccgc ccagcaccgt
      361 ctgcgacgtc cccgtcacct cggcggtctc atagtccacc atcgggaacc acagccggcc
      421 ctcatagcgc cggccctgat gggcgatcat cgcgcccgtg cattcctcgg cccagcgctt
      481 catgtcggcg tcgccgaaca tctcccagct gtcccacaaa tattcgtaga agctgtcggc
      541 atagacgtcg atcgaggcgt tgcggctggt gaactgcccc gtctccgcat gaatattggc
      601 cgccatcagc cccttgtcgg accgcatgtc gagcgcatgc ttcatcgccc gcttggccgc
      661 cgcatagtat ttcggctcgc ccgtcagttg gcccagcagg ccgaactcgg tggcgtaggt
      721 gccgatctcg gcgaggttgg tcatggggtc gcgcaccgcc cccgtcctca ggttcacata
      781 gcggtacggc aggccgtgcg gcgaggcgtc gaaggccttc aaaagacggt cggcgagatc
      841 acgcgccttg ctgagcagca ccggatcgcc gctggccaga tgcgccgaca gcaggccgcc
      901 gaccagccgg atattggttt cgaacacctg ggcgaagccg tcgatgtcga aatccagatg
      961 ggtcttgacc cagtcgaccc ccgcctggaa ctcctcgtcc agctccatga tccatagcgt
     1021 gtccagcgcc tcgaccagcg acagaccaag gtcatggccc tcgatgaaga aggactgcga
     1081 ggtcccgctg accggattga tctcgtcctt gccccatgcc cgggcgacat agtggtccca
     1141 cgcccatttg aactcggcct tcacgtcggc gccgagcgcg gcccagtcct catattgcgc
     1201 aaacgcgggc aggctgacgg cggcggcggt tccgcccatc agggcaaggg cgtggcggcg
     1261 gttcagggat cggatcatgc atggacccta gcgcgccctt ttcctttcgt cattccgggc
     1321 ggagaggcaa agcctcgcag acccggaacc cagcggcgcc gcgacggcgg cgaaacactg
     1381 ttgctgaagg gtcgccccgc cgccagcgct cccgacagat tcgcgctctc gcgcgccgct
     1441 gggttccggg tctccgctac gcttcgcccg gaatgacgaa agaaaagatc aggaactgac
     1501 cgccagccgc ggcccgttca tccacggcgg ctcgggcatg gaccggcgcg ccgtcgtctg
     1561 ggcgcgggtc cagcgttcgt gcaggtcggc gaaatagggg tcgtcttcct cgatccggcg
     1621 ctggtcgccg ctcaggaagc cgcgccggat ggtgatcagg tcgaacggcg ccccgacccc
     1681 gacattgctg cgcatcgtgg tcgagaagga gatcagcccc agcttcaccg cctcggccaa
     1741 cgggctgtcg aaggcaaggc cgctctcgag gatcggcttg ccgtatttgg cctcgccgat
     1801 ctgcagatag ggggtgtccc gcccgcactc gatgaaattg ccctgactgt agatcaggaa
     1861 cagccccatc tggccgcccc cgatctggcc gcccagcagc atggaggccg aggtgttcag
     1921 gctgtcggct tccagcgccg gcgcgatcga cagccgcacg ctgcgcaggg cgtggcccag
     1981 aacctgggcg cagcggaaca gcgtcggcgc tgtttccagc gtctcgggct cgtcgcccac
     2041 gttcagatgg acgccttcgc gggccatggc cagcgcggtc tgggtgacgg acagattgcc
     2101 ggcggtgcag atgcccagca cccgctcgcc cggccggtcg acgacgtgca gcttgcgata
     2161 agtcgagatg ctgtcgacgc ccgcattggt gcgggtgtcg gcgatcatcg ccagaccctc
     2221 ctcgaccagc atgcccacgc aataggtcat gcgg
//
LOCUS       DASVVN010000243         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_351836,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000243 DASVVN010000000
VERSION     DASVVN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351836"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VF686_03835"
     CDS             <1..621
                     /locus_tag="VF686_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8660975.1"
                     /translation="DGPKGISTFVVEKDAPGLSFGGQEKKMGWNSQPTAIVSFDDCRI
                     PAANLLGKEGDGFRYAMMGLDGGRLNIAACSLGGARLALETTLEYVNTRKQFGKSIAD
                     FQNTQFKLADMATQLDACRLMVLRGAWAMDTNHPEKTKWCAMAKRMATDVCFQIGDEA
                     LQLHGGYGYLKDYPLERIVRDLRVHRILEGTNEIMRVITAREMMRQ"
     gene            659..1708
                     /locus_tag="VF686_03840"
     CDS             659..1708
                     /locus_tag="VF686_03840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8660976.1"
                     /translation="MSESEVLTRVESGVGRITLNRPRALHALNRDMCEAMTAALLAWR
                     GDEAVKSVLIDHSGERGFCAGGDIRMIAESGAGDASEAKAFFLSEYRLNHLMFEYPKP
                     VTAIVDGIVMGGGVGISEPAEVRIATERTTYAMPETGIGLFPDVGGGWFLPRLPGQTG
                     VWLALTGARLKATDTVFLGIHSHYLPSDALEAFKAILAADPAHPADVADGLEADPGEA
                     PVEAHLEAIDRLFAFDTVEEIFTALEVDGSDWALAQLAILKTKSPQSMKVTLRQLRTG
                     ATLSTFADNMAMEYRLGGRVVRSHDFQEGVRAVIVDKDNTPNWSPAGLAGVSDTMVEA
                     LFAPLPADEEWSPLA"
BASE COUNT          307 a          523 c          648 g          267 t
ORIGIN      
        1 gacgggccga aaggcatctc caccttcgtg gtcgagaagg acgcgccggg cctgagcttc
       61 ggcgggcagg agaagaagat gggctggaac agccagccca cggccatcgt cagtttcgac
      121 gactgccgca tccctgccgc caatctgctg ggcaaggagg gcgacggctt ccgttatgcg
      181 atgatggggc tggacggcgg gcggctgaac atcgcggcct gttcgctggg cggggcgagg
      241 ctggcgctgg agacgacgct ggaatacgtc aacacccgca aacagttcgg caaatcgatc
      301 gccgacttcc agaacaccca gttcaaactg gccgacatgg cgacccagct ggacgcctgc
      361 cggctgatgg tgctgcgcgg cgcatgggcg atggacacca accatcccga gaagaccaaa
      421 tggtgcgcca tggccaagcg gatggcgacc gacgtctgtt tccagatcgg cgacgaggcc
      481 ctgcagctgc acggcggcta cggctatctg aaggactatc cgctggagcg gatcgtgcgc
      541 gacctgaggg tccaccggat tctggaaggc accaacgaga tcatgcgggt gatcacggcg
      601 cgggaaatga tgcggcagta gactggaccg tgacggcggc gcttcgtaga ggcagcgcat
      661 gagcgaatcc gaagtcctga cccgcgtcga atccggcgtg ggccgcatca ccctgaaccg
      721 gcccagggcg ctgcatgcgc tgaaccgcga catgtgcgag gcgatgacgg cggctttgct
      781 ggcgtggcgc ggggacgagg cggtcaaatc cgtcttgatc gaccattcgg gcgagcgcgg
      841 tttctgcgcc ggcggcgaca tccggatgat cgccgagagc ggcgcgggag atgcgtcgga
      901 ggccaaggcc ttcttcctca gcgaataccg gctgaaccat ctgatgttcg aatatccgaa
      961 gccggtcacg gcgatcgtcg acgggatcgt catgggcggc ggggtcggca tctcggagcc
     1021 ggccgaggtg cggatcgcca cggagcggac gacctacgcc atgcccgaga cggggatcgg
     1081 cctgtttccg gacgtgggcg gggggtggtt cctgccgcgg ctgccggggc agacgggcgt
     1141 ctggctggcg ctgacggggg cgcgactaaa agcgacggac acggtcttcc tgggcatcca
     1201 cagccactat ctgccgagcg acgcgctgga ggcgttcaag gccatactgg cggcggaccc
     1261 cgcccacccg gcggacgtgg ccgacgggct ggaggccgat ccgggcgagg cgccggtgga
     1321 ggcgcatctg gaggccattg accggttgtt cgccttcgat acggtcgagg agattttcac
     1381 ggcgctggag gttgacggat cggactgggc gctggcccag ctggcgatcc tgaagaccaa
     1441 gtcgccgcaa tcgatgaagg tcaccctgcg ccagctgcgg acgggggcga ccctgtcgac
     1501 cttcgccgac aacatggcca tggagtaccg gctgggcggc cgtgtggtgc gcagccacga
     1561 cttccaggag ggggttcgcg cggtcatcgt cgacaaggac aatacgccga actggtcgcc
     1621 ggccgggctg gcgggggtga gcgacacgat ggtggaggcg ctgttcgcgc cgctgccggc
     1681 ggacgaggag tggtcgccgc tggcctgaca gccgaggctt gctttcgtcg tgaaagccgc
     1741 gcgta
//
LOCUS       DASVVN010000244         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_456470,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000244 DASVVN010000000
VERSION     DASVVN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456470"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2860)
                     /locus_tag="VF686_03845"
     CDS             complement(38..2860)
                     /locus_tag="VF686_03845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8660977.1"
                     /translation="MKRTAALLGASLLALASPALAQAPISVPPLEYTHRELANGLDVY
                     AMPDATAGTVTVTLWYDVGGKDDPQGRSGFAHLFEHILSRKTVNLPYGQISTLVENAG
                     GSRNASTGQDFTNYYETVPPQYLETMLWTHAERMARPVVDDAVFQAERSIVQEELRQR
                     VLAPPYGRFGRFVIGDNSFDESIYRRSVIGSLEELNSAVIDDARAFHEAYYRPATATM
                     IVSGNFDPADLDRLVDQYFAGIRNPDRPVPVFERPVETPRTAPRRAEAYAPNVPLPAV
                     GVLYPGLAANHPDNAALEVLQAIMSRGDSSRLYQSLVYGKQLASNAGFGANSMEEDGS
                     ISVTATVARGKSMTDLEAALDVELARVRDEPVTAAELAEAKTELVASDLRQRETASGR
                     AFMLGQAIVSENNPRAPDERLAAIQAVTTADVQRVARTYLNDQTRVAVRYQDESARPA
                     GVPEDSWRNPAPVPTYLTVPPAILPANELLPEGQRMAPPAPGPVRAMATPRVVERTLS
                     NGLRVIVAKSTNLPIMNAQLVMAGGAAADSGQSGLASMTANLATQGAGDRSAPEIART
                     LEALGANLGGGAGADATTLFLSAPVASADQAGAVLADVVQRPTFDPAELERSRTQTVT
                     ALTVALRDPGSLASQVLNRLAFGGAAYGAPAAGTPASVGTLTRDQIVGFHDRWWRPDN
                     AALIITGGMDPEQAFAFAERTLGGWEKPSGAVPTVTNRASERPAPRIVVVDLPGAGQA
                     AVAAAVRGPRRSDPAFYPLAVVNSVIGGGQNGHLFQEVRAKRGLSYGANSQLSARVDG
                     GLLAAATQAKNESAAEVVALVLAEFDRLKSEAVTDQAVTDREAFITGGFSRAVETTGG
                     LGGVLSDAVTYGLPLTEVETYVGKINATTPEGVTAAAQAVGSDQAYVVVVGQSAMFID
                     ALRAAHPDLVVIPAAELDLNSATLGL"
     gene            complement(2929..3777)
                     /locus_tag="VF686_03850"
     CDS             complement(2929..3777)
                     /locus_tag="VF686_03850"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX8660978.1"
                     /translation="MRITVVGSINLDFVASGRFLPRPGETVTGATLARHPGGKGANQA
                     LAAQRLGADVSLTGCVGDDEMAGAALALLMAHGVELSEVVTDIAEPTGVALIAVDDAG
                     ENQIVVCAGANHRVTPEQLPARIDCPLIVQLELPIATVEAAVGRATEFVCVNLAPAAP
                     VSDQLLRRADLLVVNETEAAFYGDMLHHGGGRVVVTEGARGAVMYQRGQELARATPPR
                     ITALDATGAGDAFVGAICVALLEGMSDAEALRFACAAGAVAATRPGAQPSLPTLAEVE
                     AIITAS"
     gene            complement(3839..>4601)
                     /locus_tag="VF686_03855"
     CDS             complement(3839..>4601)
                     /locus_tag="VF686_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX8660979.1"
                     /translation="AGRTDVPVFMGADRPLRREPAGAGEFHGAEGLGDLTPFEPDAPC
                     TDGHAADAIIALVMERPEKSVAMAVLGPLTNLALALRKEPRLAERLGPVAIMGGARSE
                     GGNITASAEFNIWADPEAAAEVLATRCEAVLFGLDATHQVRATDARIAALEAVGTPMA
                     DTAASMMRFSQRVERKIVGWDAPPVHDPCPVAWLLRPNLFELKPCRIDVETQSDLTRG
                     HTAVEFRVDPATAPHRWAVSADADGVFALITEACS"
BASE COUNT          660 a         1753 c         1540 g          648 t
ORIGIN      
        1 ctccccatcg gcttcgccga cagggaggag agggagccta cagccccagc gtcgcgctgt
       61 tcaggtccag ctccgccgcc gggatcacca ccaggtcggg atgggcggcc cgcagggcgt
      121 cgatgaacat ggccgactgg cccaccacca cgacataggc ctgatccgac ccgacggcct
      181 gcgccgccgc cgtcacgccc tcgggcgtgg tggcgttgat cttgccgaca taggtctcga
      241 cctccgtcag cggcagaccg taggtgacgg cgtccgacag gaccccgccc agtccgcccg
      301 tggtctcgac cgcgcgcgag aagccgccgg tgatgaaggc ctcgcggtcg gtcaccgcct
      361 gatcggtcac cgcctcgctc ttcagccggt cgaactcggc cagcaccagg gcgaccacct
      421 cggccgcact ctcgttcttg gcctgggttg cggccgccag caggccgccg tcgacccgcg
      481 cgctcaactg ggagttggcg ccgtacgaca gaccgcgctt ggcccggacc tcctggaaca
      541 ggtgtccgtt ctggccgccg ccgatgacgc tgttgaccac cgccagcgga tagaaggccg
      601 gatccgaccg gcgcggtccg cgcacggccg ccgccaccgc cgcctgaccc gcgcccggca
      661 gatcgaccac gacgatccgt ggcgcgggcc gttcgctggc ccggttcgtg acggtcggaa
      721 ccgcgccgga cggcttctcc catccaccca gcgtccgctc ggcgaaggcg aaggcctgct
      781 ccggatccat gccgcccgtg atgatcagag cggcgttgtc gggccgccac cagcggtcgt
      841 ggaagcccac gatctggtcg cgcgtcagcg ttccgaccga ggccggcgtc ccggccgccg
      901 gcgcaccata ggccgcgccc ccgaaggcca gccggttcag cacctgcgag gccagcgagc
      961 ccgggtcacg cagggccacg gtcagggcgg tgaccgtctg cgtccggctg cgctccagct
     1021 ccgccggatc gaaggtggga cgctggacca cgtcggccag caccgcgccc gcctgatcgg
     1081 cggaggccac gggcgccgac aggaacaggg tggtcgcgtc cgcccccgcc ccgccgccga
     1141 gattggcgcc cagcgcctcc agcgtacgcg cgatctcggg ggccgagcgg tcgcccgcgc
     1201 cctgcgtcgc cagattggcg gtcatcgagg ccagacccga ctggccggaa tcagccgccg
     1261 ctccgcccgc catcaccagc tgggcgttca tgatcggcag attggtcgat ttggccacga
     1321 tgacgcgcag gccgttcgac agcgtccgct cgaccacgcg cggcgtggcc atggcccgga
     1381 ccgggcccgg cgccggcggc gccatccgct gcccctcggg cagcagttca ttggcaggca
     1441 ggatggccgg cggcacggtc agataggtcg ggaccggggc cgggttgcgc cagctgtcct
     1501 cgggcacgcc ggcgggccgg gcgctctcgt cctgatagcg gaccgccacc cgggtctggt
     1561 cgttcagata cgtccgcgcc acccgctgca cgtccgccgt ggtcacggcc tggatcgccg
     1621 ccagccgctc gtccggcgcg cgcggattgt tctccgacac gatggcctgg ccgagcatga
     1681 aggcccggcc cgaggctgtc tcgcgctggc gcaggtccga cgccaccagt tcggtcttgg
     1741 cctcggccag ttccgccgcc gtcaccggct cgtcgcgcac gcgggccagt tcgacatcca
     1801 gcgccgcttc caggtcggtc atcgacttgc cgcgcgccac cgtggcggtc acgctgatgg
     1861 agccgtcctc ctccatgctg ttcgcgccaa acccggcgtt ggacgcgagc tgctttccgt
     1921 agacgagtga ctgatagagg cgcgagctgt cgccgcgcga catgatggcc tgcagcacct
     1981 cgagggcggc gttgtccgga tggttcgccg ccagaccggg atagagcacg cccacagccg
     2041 gcagggggac gttgggcgca taggcctcgg ccctccgcgg cgccgtgcgc ggcgtctcga
     2101 ccggccgctc gaacaccggc acgggacggt ccgggttgcg gatgccggcg aaatactgat
     2161 cgaccagacg gtccagatcg gccggatcga aattgcccga cacgatcatc gttgccgtgg
     2221 ccgggcgata gtaggcctcg tggaacgccc gcgcgtcgtc gatcaccgcc gagttcagct
     2281 cctccagcga gccgatcacg ctgcggcgat agatgctctc gtcgaaactg ttgtcgccga
     2341 tcacgaaccg gccgaaccgg ccgtacggag gggccagcac ccgctggcgc agctcttcct
     2401 ggacaatcga gcgctcggcc tggaacacgg cgtcgtcgac caccggccgc gccatccgct
     2461 cggcgtgggt ccacagcatc gtctccagat actggggcgg caccgtctcg tagtagttgg
     2521 tgaagtcctg cccggtggag gcgttgcgcg acccgcccgc gttctcgacc agggtcgaaa
     2581 tctggccgta cggcaggttg accgtcttgc ggctcaggat gtgttcgaac aggtgggcga
     2641 agccgctgcg gccctgcgga tcgtccttgc cgccgacatc gtaccagagg gtgaccgtca
     2701 ccgtgcctgc ggtggcgtcc ggcatggcgt agacgtccag cccgttggcc agttcgcgat
     2761 gggtgtactc cagcggcggc accgagatcg gcgcctgggc cagggccggc gaagcgaggg
     2821 cgagcagcga ggctcccaga agggcggcgg tgcgtttcat gtcggcggtc cccggatcgg
     2881 cgtctgaaca gaggacggga ccctatcagc gcctcgcgcc gccccaagtt acgacgccgt
     2941 aatgatcgcc tcgacctcgg ccagcgtcgg cagcgagggc tgggcccccg gccgcgtcgc
     3001 cgcgacggcc cccgccgcac aggcgaagcg cagggcctct gcgtccgaca tgccttccag
     3061 cagggcgacg cagatcgcgc ccacgaaagc gtcccccgcc ccggtcgcat ccagcgccgt
     3121 gatccggggc ggcgtcgccc gcgccagttc ctgcccgcgc tggtacatga ccgccccgcg
     3181 ggctccctcg gtcaccacaa cccggccgcc gccatgatgc agcatgtctc cgtagaaggc
     3241 ggcctcggtc tcgttgacca ccagcaggtc cgcgcgccgc agcagctggt ccgagaccgg
     3301 cgcggccggg gccaggttga cgcagacgaa ctccgtcgcc cgccccaccg ccgcctccac
     3361 cgtggcgatc ggcagttcca gctgcacgat cagcgggcaa tcgatccgcg ccggcagttg
     3421 ttcgggcgtg acccggtggt tggctccggc gcaaaccacg atctggttct cgcccgcgtc
     3481 gtcgaccgcg atcagggcca cgccggtcgg ctcggcgatg tccgtgacga cttccgacag
     3541 ctccacgcca tgagccatca gcaaggccag ggccgcgccc gccatctcgt cgtcgccgac
     3601 acagccggtc aggctgacgt ccgccccaag ccgttgcgcc gccagcgcct gattggcgcc
     3661 cttgccgccc ggatgccgcg ccagggtcgc ccccgtcacc gtttcgcccg gccggggcag
     3721 gaaccggccg ctggccacga aatccagatt gatcgagccg accacggtaa ttctcactgt
     3781 cctctccccg tgtgggggag ggggaccgcg cgaagcgtgg gggagggggc cgcctgcctc
     3841 atgaacaggc ctccgtgatc agggcgaaca cgccgtccgc atccgccgaa accgcccagc
     3901 ggtgcggcgc cgtcgccgga tcgacgcgga actccaccgc cgtatgaccg cgcgtcaggt
     3961 cgctctgcgt ctccacatca atgcggcaag gcttcagctc gaacaggttg ggccgcaaca
     4021 gccaggccac cggacacggg tcatgcaccg gcggcgcgtc ccagccgacg atcttccgct
     4081 ccacccgctg cgagaaccgc atcatcgacg ccgccgtatc ggccatgggc gttccgaccg
     4141 cctccagcgc cgcgatccgc gcgtcggtcg cccgcacctg atgcgtcgca tccagcccga
     4201 acagcacggc ctcgcacctc gtggccagca cctcggccgc cgcttccgga tcggcccaga
     4261 tattaaactc agcggaggcg gtgatgttgc ccccctccga ccgggccccg cccatgatcg
     4321 ccaccggccc cagccgctcc gccagccgcg gctccttgcg cagcgccagc gccagattgg
     4381 tcagcggccc cagcacggcc atcgccacag acttctccgg ccgctccatg accagcgcga
     4441 taatggcgtc cgccgcatgg ccatccgtac acggagcatc cggttcgaac ggggtcaggt
     4501 cgcccagccc ctcggccccg tggaactccc ccgcccccgc cggctcgcgc cgcagcggcc
     4561 ggtccgcgcc catgaagacg ggcacatccg tccgccccgc g
//
LOCUS       DASVVN010000245         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_323682,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000245 DASVVN010000000
VERSION     DASVVN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323682"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF686_03860"
     CDS             <1..459
                     /locus_tag="VF686_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX8660980.1"
                     /translation="ESVDDEWHARFSATGRRYLYRILSRPGPPALDQGRVWHIRKPLD
                     AEAMDAAAQALVGLHDFTTFRDVNCQSKSPEKTLDVARVTRSGEEVHLVFEARSFLHR
                     QVRSMTGTLAEVGQGRWSVEDVAAALAARDRAACGPVAPSDGLYLTGVTY"
     gene            complement(516..653)
                     /locus_tag="VF686_03865"
     CDS             complement(516..653)
                     /locus_tag="VF686_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX8660981.1"
                     /translation="MKKIITLTLVAAALAVSACNTISGVGRDVSAAGSAVSSGAEEAK
                     N"
     gene            753..>2155
                     /locus_tag="VF686_03870"
     CDS             753..>2155
                     /locus_tag="VF686_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8660982.1"
                     /translation="MTQPANTAARRLVETLVMNGIDRVFCVPGESYLAVLDALADVRD
                     RIQVIACRHEAGAANMAEAYGKLTGKPGVCMVTRGPGATHASIGVHTAHQDSTPMILF
                     VGQIALTDRGRGAFQEVDYREVFGGLAKWATEIESPERTVEVVERAFATALQGRMGPV
                     VIALPEDTLHADGGPAPIRPVTPARSALDPAFVEQVREKLATSECPLLVLGGSGWSDA
                     ATTAIADWSERIGLPVALSFRRKDLISNDRSNYAGDLGLGPNPKLVQRVRDADLILTI
                     GARLGENPTQGYTMLDRPRTAETLIHIHPGPEELGRVWPTLLGAAADCSLAALALSTI
                     DPGRDWKAQAGTAHADFEAFIQPVQVTGAVNMSQVIRHLGEVLGDDAILTNGAGNFAA
                     WLHRFHKHRARRTQLAPTSGAMGYGYPAAIAAKSVHPDREVICIAGDGDYLMTGQEVA
                     TAVQYGINVVVVVVDNGT"
BASE COUNT          336 a          745 c          754 g          320 t
ORIGIN      
        1 gaatccgtcg acgacgaatg gcacgcgcgc ttttcggcga cagggcggcg ctatctgtac
       61 cgcatcctca gccggccggg tccgccggcg ctggatcagg gccgggtctg gcatatccgc
      121 aagccgctcg acgccgaggc catggatgcg gcggcgcagg cgctggtcgg cctgcacgac
      181 ttcaccacct tccgcgatgt gaactgccag tcgaaatcgc cggagaagac gctggacgtc
      241 gcccgcgtga cccgcagcgg cgaggaggtc catctggtgt tcgaggcgcg cagcttcctg
      301 caccggcagg tgcgctccat gaccggaacg ctggcggagg tgggtcaggg ccgctggtcg
      361 gtcgaggatg tggcggcggc gctggcggcg cgcgatcggg ccgcctgcgg acccgtggcg
      421 ccgtcggacg gcctgtatct gaccggcgtg acctactgac gcgaaaaggc ccgccggaga
      481 ccggcgggcc tttcgtctgg cgtaaaacga aatccttagt tcttggcttc ttccgcgccg
      541 gagctgacgg cgctgccggc ggccgagaca tcgcggccga cgcccgagat ggtgttgcag
      601 gccgagacgg ccagggcggc ggcgacgagg gtgagggtga tgatcttttt catgggtctt
      661 ccttgggagg cagtgtgaac cttcaccgta aacgcaagcg tggccgccgg gttgcatggc
      721 caagccgggg gtcgatctgc taaccgctgg acatgaccca gcccgcgaac accgccgccc
      781 gccgcctcgt cgaaaccctc gtcatgaacg gcatcgaccg cgtcttctgc gtgccgggcg
      841 aaagctatct ggccgtgctg gatgcactgg ccgacgtgcg cgacagaatc caggtcatcg
      901 cctgccggca tgaggcgggc gccgccaaca tggccgaggc ctacggcaag ctgaccggca
      961 aacccggagt ctgcatggtc acgcgcgggc cgggcgcgac ccacgcctcc atcggcgtgc
     1021 acacggcgca tcaggactcc acgccgatga tcctgttcgt cggccagatc gccctgaccg
     1081 accgtgggcg cggcgccttc caggaggtcg actaccgcga ggtgttcggc ggtctggcca
     1141 aatgggcgac cgagatcgag agcccggaac gcaccgtcga ggtggtcgag cgcgccttcg
     1201 ccaccgctct tcagggccgg atgggtcccg tggtcatcgc cctgccggaa gacaccctgc
     1261 acgccgacgg cggccccgcg ccgatccggc cggtgacgcc cgcgaggtcc gccctggacc
     1321 ccgccttcgt ggaacaggtg cgcgagaagc tggcgacgtc ggaatgcccg ctgctggtgc
     1381 tgggaggatc gggctggagc gacgcggcga ccaccgccat cgccgactgg tccgagcgga
     1441 tcggcctgcc cgtcgccctg tcgttccgcc gcaaggatct gatctcgaac gatcggtcca
     1501 actacgccgg cgaccttggc ctggggccca atccgaaact ggtccagcgg gtcagggacg
     1561 ccgatctgat cctgaccatc ggcgcgcggc tgggcgagaa cccgacccag ggctacacca
     1621 tgctggaccg cccccgcacg gccgaaaccc tgatccacat ccatccgggc cccgaggagc
     1681 tgggccgcgt ctggccgacg ctgctgggcg ccgcggccga ctgttcgctg gcggcgctgg
     1741 ccctgtcgac gatcgatccg ggccgcgact ggaaggcaca ggccgggacc gcccacgccg
     1801 atttcgaggc cttcatccag ccggttcagg tgaccggcgc cgtgaacatg agccaggtca
     1861 tccggcatct gggcgaggtc ctgggcgacg acgccatcct gaccaacggg gccggcaatt
     1921 tcgccgcctg gctgcaccgc ttccacaaac accgggcgcg gcgcacgcag ttggcgccga
     1981 cgtcgggggc catgggttac ggctaccccg ccgccatcgc cgccaagtcg gtccatcccg
     2041 accgcgaggt gatctgcatc gccggcgacg gcgattatct gatgaccggg caggaggtcg
     2101 ccaccgccgt ccagtacggg atcaacgtcg tggtcgtggt cgtcgacaac gggac
//
LOCUS       DASVVN010000246         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_86762, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000246 DASVVN010000000
VERSION     DASVVN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86762"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..>1550)
                     /locus_tag="VF686_03875"
     CDS             complement(45..>1550)
                     /locus_tag="VF686_03875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8660983.1"
                     /translation="IDPAICVIADAAGERPIGLGGVMGGESTGCSDDTVDVFLESAWF
                     DPIVTAQTGRTLAISSDAQYRFARGVDPAFMVPGLELATRLILDLCGGEPSEVVVAGQ
                     ALANHAPFAFDPARVGQLTGLTLDDDRIAEILTRLGFEITRGTSWTVTPPSWRRDIEG
                     PADLVEEVARIQGFDDLPSTPLPDQGAPSRGVLNPRQARVRRARRALAAMGYAEAVTW
                     SFTRQATAALFGGGDGRLLVENPIAADLDCMRPSALPNLIQAAARNAARGHADATLFE
                     IGPVYMDDSPTGQRTVIAGLVAPRSARHWGGGPEETLFGLKGDLMALLEELGAPVGSL
                     QLVQGQNRDWWHPGRSARLQLGPKTVIVEFGALHPRVLKALDADGPMLAFEIVLDAVP
                     EPRGAKSKARGLANLANLMPLTRDFAFVVEDGKAVGELTRAVAAADKALIAEVRVFDV
                     YRGAGVPEGMKSVALEVVIQPVEATLTEAEIEALSAKIVAAAARLGATLRT"
BASE COUNT          229 a          557 c          557 g          207 t
ORIGIN      
        1 ccttgcggcg gcgaggttaa cgggagattg ccgccggcgg cgactcaggt ccgcagggtc
       61 gcgcccaacc tggccgccgc cgccacgatc ttcgctgaca gcgcctcgat ctccgcctcg
      121 gtcagggtcg cttcgacggg ctggatcacg acctccagcg ccaccgattt catgccctcg
      181 ggcaccccgg cgccgcgata cacgtcgaac acccgcacct cggcgatcag ggccttgtcg
      241 gccgcggcca cagcgcgcgt cagctcgcca accgccttgc cgtcctcgac cacgaaggcg
      301 aagtcacggg tcaggggcat cagattggcg aggttggcga gaccgcgcgc cttggacttc
      361 gccccgcgcg gctcaggcac ggcgtccaga acgatctcga aggccagcat cggcccgtcg
      421 gcgtccagcg ccttgagcac gcgcggatgc agggcgccga attcgacgat gacggtcttg
      481 ggacccagct gcaggcgcgc cgagcggccg ggatgccacc agtcgcggtt ctggccctgc
      541 accagctgca gcgagccgac cggcgcgccc agctcttcca gaagcgccat caggtcgccc
      601 ttgagaccga acagggtctc ctcgggcccg ccgccccagt ggcgcgccga tcgcggcgcc
      661 accaggccgg ctatgacggt gcgctgaccg gtcgggctgt cgtccatata gacggggccg
      721 atctcgaaca gggtggcgtc ggcgtggccg cgcgcggcgt tgcgggcggc ggcctggatc
      781 agattgggca gggccgaggg ccgcatgcag tccagatcgg ccgctatggg gttctcgacc
      841 agcaaccggc cgtcgccgcc gccgaacagg gccgcggtcg cctgtctggt gaaggaccag
      901 gtcacggcct cggcatagcc catggcggcc agcgcgcggc gggcgcggcg gacccgggcc
      961 tgacgcgggt tcagcacgcc gcgtgacggc gcgccctgat cgggcagcgg ggtcgacggc
     1021 aggtcgtcga agccctggat gcgcgcgact tcctcgacca gatcggcggg cccctcgatg
     1081 tcgcggcgcc acgatggcgg cgtcacggtc cacgacgtcc cgcgcgtgat ctcgaagccg
     1141 agccgggtca ggatttcggc gatccggtcg tcatccagcg tcaggccggt caactggccg
     1201 acccgggccg ggtcgaaggc gaagggcgcg tgattggcca gcgcctgccc cgccacgacg
     1261 acctcggacg gctcgccgcc gcacaggtcg aggatcagac gcgtggccag ctccaggccc
     1321 gggaccatga aggccggatc cacgccgcgc gcgaaccggt actgggcgtc ggagctgatc
     1381 gccagcgtgc ggcccgtctg ggccgtgacg atcggatcga accaggcgct ttcgaggaag
     1441 acgtcgacgg tgtcgtccga acagccggtc gactccccgc ccatgacgcc gcccagaccg
     1501 atgggccgtt cgccggcggc gtcggcgatg acgcaaatgg ccgggtcgat
//
LOCUS       DASVVN010000247         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_456800,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000247 DASVVN010000000
VERSION     DASVVN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456800"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VF686_03880"
     CDS             <1..930
                     /locus_tag="VF686_03880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX8660984.1"
                     /translation="RVRLVTAGEDTEVDKTVVERLAEPITHMLRNAIDHGLESPDDRL
                     AAGKPAEGSVRLAALHRSGRIVIEISDDGRGINRERVKGIATSKGLIAEDVVLSDEEI
                     DNLIFLPGFSTAETISDVSGRGVGMDVVRRSIQALGGRISISSNPGKGSTFTLSLPLT
                     LAVLDGMVVTAADQTLVAPLTTIVESLTPRAQDVHHVGGHDAVIRFRDEFVPLIDVAL
                     TMGFRDAPVPSGGGVAVVIETEAGLRAALLFDAIQGQRQVVIKSLETNYQQVEGVAAA
                     TILGDGRVALILDVDAIVGNARRRARPDFKLAS"
     gene            933..1394
                     /locus_tag="VF686_03885"
     CDS             933..1394
                     /locus_tag="VF686_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX8660985.1"
                     /translation="MSDTPSTSASRELIAFRIGEQEFCVDIMSVREIRGWTPATPLPR
                     APAFMKGVINLRGAVLPIIDLGARLGLRTAEPTARHVIMVAHIGGRMVGLLVDAVSDI
                     IQLTDELVQPTPDVASDHVKSFVKGIFSVEGRMVSLLELEWILPETEAEAA"
     gene            1391..2257
                     /locus_tag="VF686_03890"
     CDS             1391..2257
                     /locus_tag="VF686_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HEX8660986.1"
                     /translation="MTHAAGRASLVEGEFVFTAEDFRQIAEILHGHAGIALNEGKAAL
                     VYSRLAKRLRILGLRSFRDYCALVQGVDGVDERQAMTAALTTNVTRFFRESHHFDHLR
                     DVVMPGLAERARRGGRVRLWSAACSNGQEPYSMAMTVLSVLPEAADLDVRILATDIDP
                     NMVAEGRAGLYSEEALAPAPPLLRHRWFDKAPAHGPNMLSANATLRRLVSFKELNLIG
                     DWPMRGKFDVIFCRNVVIYFDDATQERVWGRFLPLMTPDATLFIGHSERVTGAATARL
                     STTGLTTYRMAA"
     gene            2254..3300
                     /locus_tag="VF686_03895"
     CDS             2254..3300
                     /locus_tag="VF686_03895"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX8660987.1"
                     /translation="MTISVLVVDDSPTMRGLITAALRRDPEIEVVGSAADPFEAREAI
                     KRLNPDVITLDVEMPNMNGLEFLQKIMRLRPMPVVMVSTLTQAGAEVTLAALELGAVD
                     AVGKPGAGTTVQEAFADLAAKVKVAARSRVRAGSGAGAEARSAPGNWRIGSDHVLAIG
                     ASTGGVEALLTIISAFPANCPATVITQHMPATFTSSFAARLDKASAATVREAADGDVL
                     EQGCVYLAPGGEAHLEVGGITPRCRLVRSDTVSGHRPSVDVLFRSVAHLRRPITGVIL
                     TGMGRDGAAGLLEMRQAGASTLGQDEASSVVYGMPRAAFEMGAVQRQLPLGRMAPAIL
                     ELCGDASSAARPIA"
     gene            3311..3700
                     /locus_tag="VF686_03900"
     CDS             3311..3700
                     /locus_tag="VF686_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8660988.1"
                     /translation="MPAASAIHTLIVDDQASMRALIRTSMQALGFREFREAGDGEAGL
                     REMITRPAHLIISDFNMPNLDGLGLLRAVRAHPPTAGAAFIMLTGRADRELVQRAVQF
                     GVNNYLVKPFTVAQLKDKVEGVFGPLT"
     gene            3697..4239
                     /locus_tag="VF686_03905"
     CDS             3697..4239
                     /locus_tag="VF686_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505894.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX8660989.1"
                     /translation="MTRVETADAVRRIHVGQGEHFVTDDPGVMLTTILGSCVAMCLRD
                     SGSRIGGMNHFLLPEGAGSGADAGRRYGAYAMEVLINDCLKKGARRDRLEAKLFGGGR
                     MFDALRDVGLANADFAERFLRDEGIRVVGGSLRGSGGRRVQYWPATGRALQRVVTDRV
                     VPAVARVPQAAVEAGALELF"
     gene            4239..>4473
                     /locus_tag="VF686_03910"
     CDS             4239..>4473
                     /locus_tag="VF686_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660990.1"
                     /translation="MSAAPSCSLLSALSAELAAMMETVEPLCGMAANHVRNSQGEARA
                     RALVDAQAIDDLSQRLTALSDLTGAVARGEDVET"
BASE COUNT          668 a         1631 c         1467 g          707 t
ORIGIN      
        1 cgcgtgcgtc tggtcaccgc cggcgaggac accgaggtcg acaagacggt ggtcgaacgc
       61 ctcgccgagc ccatcaccca catgctccgc aacgccatcg atcacgggct ggaaagcccc
      121 gacgatcgcc tcgccgccgg caagcccgcc gagggctcgg tgcggctcgc cgccctgcac
      181 cgctccggcc ggatcgtgat cgagatcagc gacgacggac gcggcatcaa ccgcgaacgg
      241 gtcaagggca tcgccacgtc gaagggcctg atcgccgagg atgtcgtgct gtccgacgag
      301 gagatcgaca acctgatctt cctgccgggc ttctcgacgg ccgagaccat ctcggacgta
      361 tcgggaaggg gcgtgggcat ggatgtcgtg cgccgctcga tccaggcgct gggcggccgc
      421 atcagcatct cgtcgaaccc cggcaagggc tccaccttca ccctcagcct gccgctgacc
      481 ctggcggtgc tggacgggat ggtggtcacc gccgccgacc agaccctcgt tgcgcccctc
      541 acgactatcg tagaaagcct gacgccccgg gcgcaggacg tgcaccacgt cggcggccat
      601 gacgcagtga tccggttccg cgacgagttc gtgcctctca tcgacgtcgc cctgacgatg
      661 ggcttccgcg atgcgccggt cccgtccggc ggcggcgtgg cggtggtgat cgagaccgag
      721 gccggcttgc gcgccgccct gctgttcgac gccattcagg gtcagcggca ggtcgtcatc
      781 aaaagcctcg agaccaacta ccagcaggtc gagggcgtcg ccgccgcgac cattctgggc
      841 gacggccggg tcgccctgat cctcgacgta gacgccatcg tgggcaacgc ccgccgccgg
      901 gcgcgtcctg acttcaaact cgcgagctga tcatgtccga caccccgagc acctccgcct
      961 cgcgcgaact catcgccttc cgcatcggcg agcaggagtt ctgcgtggac atcatgtccg
     1021 tgcgggagat ccgcggctgg accccggcca cgcctctgcc ccgcgcgccc gccttcatga
     1081 agggcgtcat caacctgcgc ggcgccgtgc ttccgatcat cgacctgggc gcccgtctcg
     1141 gcctgcgcac cgccgagccc acggcccggc acgtcattat ggtggcgcac atcggcggcc
     1201 gcatggtggg actgctcgtc gacgccgtct ccgacatcat tcagctcacg gacgaactgg
     1261 tccagcccac gcccgacgtc gccagcgatc acgtgaaaag cttcgtcaag ggcatcttct
     1321 ccgtcgaggg ccggatggtc agcctgctcg agctggaatg gatcctgccc gaaaccgaag
     1381 ccgaagccgc atgacccacg ccgccggccg cgcgtccctc gtcgaaggcg aattcgtctt
     1441 cacggccgag gacttccgcc agatcgccga aatcctgcac ggccacgcgg gcatcgcgct
     1501 gaacgagggc aaggccgccc tggtctattc gcggctggcc aaacggctgc gtatcctcgg
     1561 cctgcgcagc ttccgcgact attgcgccct cgttcaaggc gtcgatggcg tggatgagcg
     1621 tcaggcgatg acggcggccc tgaccaccaa cgtcacgcgc ttcttccgcg agtcgcacca
     1681 tttcgatcac ctgcgtgacg tggtcatgcc cggtctcgcc gagcgcgctc gccgcggcgg
     1741 gcgggtgcgg ctgtggtccg cggcctgctc gaacgggcag gagccctatt cgatggccat
     1801 gacggtcctg tccgtgctcc ccgaggcggc cgatctggac gtgcggatcc tggccaccga
     1861 catcgatccc aacatggtcg cggaaggccg cgccggcctc tattccgaag aagccctcgc
     1921 gcccgcgccg cccctgctgc gccaccgttg gttcgacaag gcgccggctc acggcccgaa
     1981 catgctttcg gccaatgcga cccttcggcg gctggtgtcg ttcaaggaac tgaacctgat
     2041 cggcgactgg ccgatgcgcg gcaagttcga cgtcatcttc tgtcgcaacg tcgtgatcta
     2101 tttcgacgat gcgacccagg agcgggtgtg gggccggttc ctgcccctga tgacgccgga
     2161 cgccaccctc ttcatcggcc attccgagcg cgtcaccggc gcggccaccg cgcggctcag
     2221 cacgaccggc ctgacgacct accggatggc cgcatgacga tctccgtcct ggtcgtcgac
     2281 gactctccca ccatgcgcgg cctgatcact gccgctcttc gccgcgatcc cgagatcgag
     2341 gtggtcggct ccgcggccga cccgttcgag gcgcgcgagg cgatcaagcg gttgaacccc
     2401 gatgtcatca ccctcgacgt cgagatgccg aacatgaacg gcctcgaatt ccttcagaag
     2461 atcatgcggc tgcgcccgat gccggtcgtc atggtctcga ccctgacgca ggcgggggcg
     2521 gaggtgacgc tggccgcgct cgagctcggc gccgtcgacg cggtcggcaa gccgggggcc
     2581 gggacgacgg tgcaggaggc cttcgccgat ctcgccgcca aggtgaaggt cgccgcccgc
     2641 tcgcgcgtca gggccggatc gggcgcgggc gccgaagcgc ggtccgctcc cggaaactgg
     2701 aggatcggtt ccgaccatgt gctggccatc ggcgcctcca ccggcggagt cgaggccttg
     2761 ctgaccatca tctccgcgtt tccggccaac tgtcccgcga ccgtgatcac ccagcatatg
     2821 ccggcgacct tcaccagcag cttcgccgcg cgcctcgaca aggcctcggc ggcgacggtg
     2881 cgagaggcgg ctgacggcga cgtgctcgaa cagggctgcg tctatctggc gccgggcggc
     2941 gaagcccatc tcgaggtcgg cgggatcacg ccgcgctgcc gtctcgtccg ctccgacacc
     3001 gtcagcggtc atcggccctc ggtcgacgtc ctgttccgtt ctgtcgccca cctgcgccgt
     3061 ccgatcacgg gagtgatcct gaccggcatg ggccgtgacg gcgcggcggg tctgctcgag
     3121 atgaggcagg ccggcgccag caccctcggt caggatgagg cgagcagcgt cgtctacggc
     3181 atgccccgcg ccgctttcga aatgggcgcg gtccagcgcc agttgcccct cggccgcatg
     3241 gcccccgcca tccttgaact ctgcggcgac gcctcctccg ccgcccgtcc catcgcctga
     3301 aggtccagtc atgcctgccg cttccgccat tcacacgctc atcgtcgacg accaagcctc
     3361 catgcgcgcc ctgatccgga cgagcatgca agcgctcggc ttccgcgagt tccgcgaggc
     3421 gggcgacggc gaggcgggcc tgcgcgagat gatcacccgg cccgcccacc tgatcatctc
     3481 ggacttcaac atgccgaacc tggacgggct gggcctgctg cgcgccgtcc gcgcccatcc
     3541 gcctacggcc ggcgcggcct tcatcatgct caccggccgg gccgaccgcg aactggtcca
     3601 gcgcgccgtc cagttcggcg tcaacaacta cctcgtcaag ccgttcaccg tcgcccagct
     3661 gaaggacaag gtcgagggcg tcttcggtcc gttgacatga cccgggtgga gaccgccgac
     3721 gccgtccgcc gcatccacgt cggccagggc gagcatttcg tcaccgacga tccgggcgtg
     3781 atgctgacca ccattctggg cagctgcgtc gccatgtgcc tgcgcgattc cggcagccgg
     3841 atcggcggca tgaaccactt cctgctcccg gagggggccg gctcgggcgc cgacgcaggc
     3901 cggcgttacg gcgcgtatgc gatggaagtc ctgatcaacg actgcctcaa gaagggcgcg
     3961 cgccgcgacc ggctcgaggc caagctgttc ggcggcgggc ggatgttcga cgctctcagg
     4021 gacgtcggtc tggccaacgc cgatttcgcc gaacgcttcc ttcgcgacga gggaatccgc
     4081 gtggttggcg gaagtctgcg cggatccggc gggcgccgcg tccagtactg gccggcgacg
     4141 ggacgagccc tgcagcgggt cgtcaccgat cgcgtcgtcc ccgctgtcgc ccgtgtcccg
     4201 caggcggccg tcgaggccgg cgccctggag ctgttctgat gtccgccgcc ccgtcctgct
     4261 cgctcctttc ggcgctctcg gccgagctgg ccgcgatgat ggaaacggtt gaaccgctct
     4321 gcggaatggc cgcgaaccac gtccgcaaca gccagggcga ggcccgggcc cgcgccctcg
     4381 tggacgccca ggcgatcgac gatctgagcc agcgtctgac cgccctcagc gatctaaccg
     4441 gcgcggtcgc gcggggagaa gacgtcgaga ccg
//
LOCUS       DASVVN010000248         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_87060, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000248 DASVVN010000000
VERSION     DASVVN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87060"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1467)
                     /locus_tag="VF686_03915"
     CDS             complement(205..1467)
                     /locus_tag="VF686_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEX8660991.1"
                     /translation="MSALSRLRTHRAALAFIFVTAVLDIVAMGIIIPVLPRLIEDFVG
                     SNAQAGVINGVFVALWAGMQFVCSPIIGSLSDKYGRRPVILISCAGLALDYVLMAVAP
                     DLWWLALGRIIAGITSSSFTTVYAYMADITAPEKRARAYGLIGAAFSGGFVLGPVLGG
                     FLGEFGPRVPFWFAAGLSTVAFLYGLLVLPESLPVEKRMDFSWKRANPVGSLILLRRH
                     PELSGLAMVNFLLYFAHHVFSAVFVLYAAWRYGWGPREVGLLLALVGVLDMVVQGVLV
                     GPVVKRLGDRSTMVLGLVGGTIGIAAMGWAPDGLWFITAMLPNALWGLAMPTLQSLMT
                     RRVGEDEQGQLQGANMSVASIAGVMSPLFFGWVYSLSVGEFAVIQMPGLSFYLAAVVL
                     LLAAVIGWLVGRGAERRDAAPVEAPPTP"
     gene            1534..1701
                     /locus_tag="VF686_03920"
                     /pseudo
     CDS             1534..1701
                     /locus_tag="VF686_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          332 a          596 c          560 g          213 t
ORIGIN      
        1 cgaaactggc cttcatcgcc tgatcgtccc ccggcacgac ccggttatca gggaagaccc
       61 cgtcctgggc attggccgca ttgaactggc cgcaggcggc aagcaaaagg gcggcggcga
      121 tggcgagcgt atcggtcttc atggtcatcc ggttcggtca gaaacgcaca agcgacgcca
      181 ttccgcaccg gcttttggac cggatcaagg cgtcggcggg gcctcaacgg gcgcggcgtc
      241 gcgacgttcc gccccgcgcc cgaccagcca gccgatgacc gccgccagca gcaggacgac
      301 agccgccaga tagaaggaca ggcccggcat ctgaatgacc gcgaactcgc ccacggacag
      361 cgaatagacc cagccgaaga acaacggcga catgacgccg gcgatggagg cgacgctcat
      421 gttcgccccc tgaagctggc cctgctcgtc ctcgccgacg cggcgggtca tgagcgactg
      481 aagcgtcggc atggcgaggc cccacagggc gttgggcagc atggcggtga tgaaccacag
      541 cccgtccggc gcccagccca tggccgcgat cccgatcgtg ccgccgacca gacccagcac
      601 catggtcgac cggtccccca gccgcttgac gaccggcccg accagaacgc cctgcaccac
      661 catgtccagc acgccgacga gcgccagcaa caggccgacc tcgcgcgggc cccagccgta
      721 acgccaggcg gcgtacagca cgaagaccgc cgagaagacg tggtgggcga aatagagcag
      781 gaagttcacc atcgcgaggc cgctgagctc cggatgacgg cgcagcagga tcagggagcc
      841 gacgggattg gcccgcttcc agctgaaatc catccgcttc tcgaccggca ggctctcggg
      901 caggaccagc aggccgtaca ggaaggccac cgtcgacagg ccggcggcga accagaaggg
      961 cacccgcggg ccgaactcgc cgaggaagcc acccagcacc ggtcccagca cgaagccgcc
     1021 cgagaaggcc gcgccgatca ggccataggc ccgggcccgt ttctcgggcg cggtgatgtc
     1081 ggccatatag gcgtagacgg tggtgaagct ggacgaggtg atccccgcga tgatgcggcc
     1141 cagcgccagc caccacaggt cgggcgccac ggccatcagc acgtaatcca gcgccagacc
     1201 cgcacagctg atcaggatga ccggccggcg tccgtacttg tccgacagcg agccgatgat
     1261 cggcgaacag acgaactgca tcccggccca cagggcaacg aacacgccgt tgatcacccc
     1321 ggcctgtgcg ttcgagccga cgaagtcctc gatcagccgg ggcaggaccg ggatgatgat
     1381 ccccatggcc acgatatcca gcaccgccgt cacgaagatg aaggcgagcg cggcgcggtg
     1441 ggtgcggagg cggctgaggg cggacatggg aggccttgct ggggacgggc cttctagccg
     1501 acccggacgc gcccgtcagc cctgtgctac cgtcggggcg gaggccccat gaccaacttc
     1561 cttcaggtga cgcccttcct gcacgtcccg gacctgcaga aggcgctgga cttcttcacg
     1621 gacattctcg gcttcaccgt gccgttccgg cagccgggct atgcctacat ccaccgggag
     1681 acggtcggat tccggctgct g
//
LOCUS       DASVVN010000249         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_403728,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000249 DASVVN010000000
VERSION     DASVVN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403728"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..919
                     /locus_tag="VF686_03925"
     CDS             98..919
                     /locus_tag="VF686_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX8660992.1"
                     /translation="MKIGIVNASSRFNRARADAVEAWFAANIPDGSVKVVFHPASFGK
                     HGHFGGDDASRASAFVEYANDPRLDAVWFARGGYGSCRIAEAVLPRLEPAARKKRYLG
                     YSDAGSILALLYKAGFPHVAHGPMASDAVRNDATAWRAINWLKSGDRISWEPSLADDP
                     RPAAAFNLSILDALVGTALEPDLTGHVLMLEDVSEPLYRIDRMMFHLTGQASIRRVAG
                     IRMGRVSDIVENDPDFGLTAEEIARYWCQRSGIPYLGAADIGHDRENKVVPFGHR"
     gene            1150..>1687
                     /locus_tag="VF686_03930"
     CDS             1150..>1687
                     /locus_tag="VF686_03930"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX8660993.1"
                     /translation="MNRRSLLIRTAALASVLGGAWWARDHLLWPKPELAFAAGGATPW
                     MPYARRGSSPTVTVRLGGRQVVALIDSGAQYSVIDRALFAELPDTGRSLFDMPLVAYG
                     VGGGAQVGRGTALDLDLAGLAVRGLRAAILDLGPLASSQGLGTPLILGQDLLGEAVLA
                     LDPARRHSRLVAPEAFVRP"
BASE COUNT          229 a          575 c          603 g          280 t
ORIGIN      
        1 gccgcagaag aaatagtcgt cttgattcat gagggcgaaa ccggggaatg tccggcggcc
       61 tgactagcac gggcgacgcc tgaggccaga cacgtccatg aagatcggca tcgtcaacgc
      121 cagttcgcgc ttcaaccgcg cccgcgccga cgccgtcgag gcttggttcg ccgccaacat
      181 tcccgacggc tcggtcaagg tggtgttcca cccggccagt ttcggcaagc atggtcattt
      241 cggcggcgac gacgccagcc gggcctcggc cttcgtcgaa tacgccaacg atccgcggct
      301 ggacgcggtc tggttcgcgc gcggaggcta tggctcgtgc cggattgccg aggctgtgct
      361 gccccggctg gagccggcgg cgcgcaagaa gcggtatctg ggctattccg acgccggcag
      421 cattctggcc ctgctgtaca aggccggctt tccgcatgtg gcgcacggcc cgatggcctc
      481 cgacgcggtc cgcaacgacg cgacggcctg gcgggccatc aactggctga agtcgggcga
      541 ccggatttca tgggagccgt cgttggcgga cgatccgcgt ccggcggccg ccttcaacct
      601 cagcattctc gacgccctgg tcggcacggc tctggaaccc gacctgacag gccacgtcct
      661 gatgctggag gacgtgtcgg aaccgctcta ccggatcgac cggatgatgt tccatctgac
      721 cggtcaggcc tccatccggc gggtggcggg catccggatg gggcgggtat cggatatcgt
      781 cgagaacgat cccgacttcg gtctgacggc cgaggagatc gcccgctact ggtgccagcg
      841 gtcggggatt ccgtacctgg gcgccgccga catcggccat gaccgcgaga acaaggtggt
      901 tccgttcggc cacaggtagc cggccttcgg ttcgcgcagg cggggaaccg tatcgacacc
      961 ggtggcatcc caaggccaga tgcggccagc gagcgggtga gtccttcccc gcctgcaccg
     1021 cgcccttcgg gggatgggcg cgcggtggcg gtccggtctg cgtcgggggg cgcggatcgg
     1081 gccgtctccc accaccgcga caaaggcgcc gctggcgtca gcacgggccg cacgccatct
     1141 tcatcggcaa tgaaccgccg ctccctcctg atccgcacgg ctgcccttgc gtccgtcctc
     1201 ggcggcgcct ggtgggcgcg cgaccatctg ctgtggccga agccggaact ggccttcgcg
     1261 gcgggcggcg cgacgccctg gatgccctat gcgcggcggg gttcttcccc caccgtgacg
     1321 gtgcgtctgg gcggacggca ggtcgtggcc ctgatcgaca gcggcgccca gtattccgtc
     1381 atcgaccgcg ccctgttcgc cgaactgccc gacaccgggc ggtcgttgtt cgacatgccg
     1441 ctggtcgcct atggcgtcgg cggcggtgcg caggtcgggc gcggcacggc gctggacctc
     1501 gatttggccg ggctggcggt caggggcctg agggcggcga tcctcgatct cgggccattg
     1561 gcctcgtccc aggggttggg gacgccgctg atcctcgggc aggacctgct gggcgaggcc
     1621 gtgctggcgc tcgacccggc ccggcggcac agtcgtctgg tcgcgcctga ggccttcgtg
     1681 cgcccgc
//
LOCUS       DASVVN010000250         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_429767,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000250 DASVVN010000000
VERSION     DASVVN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429767"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VF686_03935"
     CDS             <1..919
                     /locus_tag="VF686_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8660994.1"
                     /translation="LGAADIVVGTPPIAFTFGLGGLVVFPAAVGAATAFPDRPGFDAL
                     AETVARHRATALFTAPTGYRALLKLAGAHDLSSLRTCVSAGEALPAATSDAWHEATDV
                     RIIDGIGSTEMIHVFISARGDAIRPGSTGKAVPGYQARIVDEAGLPLPPGETGRLAVR
                     GPTGCRYLDDERQAAYVRDGWNLTGDVYRLDEDGYFWFVARSDDMIISSGYNIGAPEV
                     EHALLRHPAVAEAAVIGAPDPERGQIVQAFVVLNEGRTPSDALRAELQTFVKGVIAPY
                     KYPRGLDFVDALPKTQTGKLQRFRLKEPT"
     gene            916..1338
                     /locus_tag="VF686_03940"
     CDS             916..1338
                     /locus_tag="VF686_03940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8660995.1"
                     /translation="MSGQIFTAERRVRFADCDAAGIVFFPRYFEMLNGVVEDWFAGPL
                     EASFRELHVNRHVSVPTAAVEARFIAPSRLEDELTFELTVTKLGGASCGLRHRISAGV
                     QLRFEATQTIVFVGASLKPEPWPEAMRARIAPFVETAP"
     gene            1335..1730
                     /locus_tag="VF686_03945"
     CDS             1335..1730
                     /locus_tag="VF686_03945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8660996.1"
                     /translation="MNRVLLPEGWPRPKGYSNGVEASGRMVFVAGQIGWTPEGVFAET
                     DFAGQFRQTIQNTLAVLTEAGAGPEHIVRMTWYVVDKAEYLSALREIGAVWRELIGPH
                     YPAMAVVEVKGLIEDAARIEIETTAVVPA"
     gene            1769..>2192
                     /gene="cysD"
                     /locus_tag="VF686_03950"
     CDS             1769..>2192
                     /gene="cysD"
                     /locus_tag="VF686_03950"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX8660997.1"
                     /translation="MRLHDDVLGALEAEAIHIFREVAAECARPVLLFSGGKDSAVLLH
                     LARKAFFPAPPPFPLLHVDTTWKFREMYAFRDRAAAEAGMELLVHRNPEAEAEGINPF
                     DHGASVHTDLWKTQGLKQALDLHRFDAAFGGARRDEEKS"
BASE COUNT          326 a          813 c          712 g          341 t
ORIGIN      
        1 gctcggcgcg gcggacatcg tcgtcggcac cccgcccatc gccttcacct tcggactggg
       61 aggcctggtg gtctttccgg ccgccgtcgg cgcggccacg gccttccccg atcggcccgg
      121 cttcgacgcc ctggccgaga ccgtcgcccg ccaccgcgcc acggccctgt tcaccgcccc
      181 gaccggctat cgcgccctgc tgaagctggc cggcgcccac gacctgtcgt ccctgcgcac
      241 ctgcgtctcg gccggagagg ccctgcccgc cgccacgtcc gacgcctggc acgaagccac
      301 tgacgtccgc atcatcgatg gcatcggctc gaccgagatg atccacgtct tcatctcggc
      361 ccgcggcgac gccatccgcc cgggctccac cggcaaggcg gtgccgggct atcaggcccg
      421 catcgtcgac gaggcgggcc tgcctctgcc cccaggggaa accggccgcc tcgccgtgcg
      481 cggtccgacc ggctgccgct atctcgatga cgaacggcag gccgcctatg tccgggacgg
      541 ctggaacctg accggcgacg tctaccggct ggatgaggac ggctatttct ggttcgtggc
      601 ccggtccgac gacatgatca tctcgtccgg ctacaacatc ggcgcgcccg aggtggaaca
      661 cgccctgctg cgccaccccg ccgtggccga ggccgccgtc atcggcgcgc ccgatcccga
      721 gcgcggccag atcgtccagg ccttcgtggt cctgaacgaa ggccgcaccc cgtccgacgc
      781 cctgcgcgca gagctccaga ccttcgtaaa gggggtcatc gccccctaca aatatccgcg
      841 cggcctggat ttcgtggacg ccctgcccaa gacccagacc ggcaagctgc aacgcttccg
      901 gctgaaggag ccgacatgag cggccagatc ttcacggccg aacgccgcgt ccgtttcgcc
      961 gactgcgacg cggcgggcat cgtgttcttt ccccgctatt tcgagatgct gaacggggtg
     1021 gtggaggact ggttcgccgg gccgcttgag gcctcgttcc gcgaactgca tgtgaaccgc
     1081 cacgtcagcg tcccgaccgc ggctgtcgaa gcccggttca tcgccccctc gcggctggag
     1141 gacgaactga ccttcgagct gaccgtcacg aagctgggcg gcgcctcctg cggcctgcgc
     1201 caccggatca gcgccggcgt ccagctgcgg ttcgaggcca cccagaccat cgtcttcgtc
     1261 ggagcctcgc tgaagcccga gccctggccc gaggccatgc gcgcccgcat cgcacccttc
     1321 gtggagaccg ccccgtgaac cgtgtcctgc tgcccgaagg ctggccccgg ccgaagggtt
     1381 attcgaacgg cgtcgaggcc agcggacgca tggtcttcgt cgccggccag atcggctgga
     1441 cgcccgaggg cgtgttcgca gaaacggact tcgctggcca gttccggcag acgatccaga
     1501 acaccctcgc cgtcctgacc gaggccggcg ccggtccgga acacatcgtc cgcatgacct
     1561 ggtacgtggt cgacaaggcc gaatacctgt ccgccttgcg cgagatcggt gcggtctggc
     1621 gcgagctgat cggtccccac tatcccgcca tggccgtggt cgaggtcaag ggactgatcg
     1681 aggacgcggc ccgtatcgag atcgagacca ccgccgtcgt ccccgcgtga gggttggcgc
     1741 cgcgcgattc cggggccaaa gctgaaccat gcggctgcac gacgacgtcc ttggcgccct
     1801 tgaagccgag gccattcaca tcttccggga ggtcgccgcc gaatgcgcgc ggcccgtgct
     1861 gctgttctcg ggcggcaagg attccgccgt cctgctgcat ctggcgcgca aggccttctt
     1921 ccccgccccg ccgcccttcc cgctgctgca cgtcgatacg acatggaaat tccgcgagat
     1981 gtacgccttc cgcgaccggg cggcggcgga ggccgggatg gagctgctgg tccaccgcaa
     2041 tccggaagcc gaggccgagg ggatcaatcc gttcgaccac ggggccagcg ttcacactga
     2101 tctatggaag acgcaggggc tgaagcaggc gctggacctt caccgcttcg acgcggcctt
     2161 cggcggcgcg cggcgcgacg aggaaaagag cc
//
LOCUS       DASVVN010000251         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_457000,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000251 DASVVN010000000
VERSION     DASVVN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457000"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..272)
                     /locus_tag="VF686_03955"
     CDS             complement(75..272)
                     /locus_tag="VF686_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8660998.1"
                     /translation="MAESDLDRLKARRVTALYRLDLIARGAQLSYEDGTPIDMTSEKD
                     RLESIVADLDRRIEILERPLN"
     gene            complement(272..1660)
                     /gene="trmFO"
                     /locus_tag="VF686_03960"
     CDS             complement(272..1660)
                     /gene="trmFO"
                     /locus_tag="VF686_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX8660999.1"
                     /translation="MSTIEPIHVVGGGLAGSEATWQIAQAGVPVILHEMRPVRGTDAH
                     QTDGLAELVCSNSFRSDDWEYNAVGLLHAEMRALDSIIMSCGDAHQVPAGGALAVDRD
                     GFSQAVTAKLEAHPLITIVREEIAGLPPEAWDNVIVATGPLTSPALAAAILETTGEES
                     LSFFDAIAPIVHADSIDFDIAWRQSRYDKEGPGGDAAAYVNCPMNREQYEAFIDALLN
                     GPKAEFKDWENVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPQVKSYAIAQL
                     RQDNALGTLFNMVGFQTKLKHGAQAEVFRMIPGLQDAQFARLGGLHRNTFLNSPKLLD
                     RQLRLKAMPRLRFAGQVTGVEGYVESAAMGLLTGRLAAAERLGRPLEAPPVHTAMGAL
                     VEHITGGHLAGPGGISKFQPMNINYGLLPPLEAPKVDEAGVKIPMKERGRAKKRLMSI
                     RALDALKAWRDA"
BASE COUNT          257 a          548 c          658 g          297 t
ORIGIN      
        1 ggatgagccc gcccacgatc cagaacatgt cggtcgcccc cttcatccgg ccatggccca
       61 ggcgttgtcc cccatcagtt cagggggcgc tcgaggattt cgatgcggcg gtcgagatcc
      121 gcgacgatgg attccagccg gtccttctcg gaggtcatgt cgatcggcgt cccgtcctcg
      181 taggacagct gggcgccgcg ggcgatcagg tcgagccggt acagggcggt gacgcgccgg
      241 gccttcaggc ggtcgaggtc gctctccgcc atcacgcgtc ccgccatgcc ttcagcgcat
      301 cgagcgcgcg gatgctcatc agccgtttct tcgcccgccc gcgctccttc atcggaatct
      361 tgaccccggc ctcgtcgact ttcggagcct cgagcggtgg cagcaggccg tagttgatgt
      421 tcatgggctg gaatttggag atgccgccgg ggcccgcaag gtggcccccg gtgatgtgtt
      481 cgacgagggc gcccatggcg gtgtggacgg gcggagcctc caacgggcga ccaagccgtt
      541 cggcggcggc gaggcgaccg gtgagcaggc ccatggcggc gctctcgaca taaccctcga
      601 cgcccgtaac ctgaccggcg aagcggaggc gcggcatcgc cttgaggcgc agctgccggt
      661 ccagcagctt cgggctgttc aggaaggtgt tgcggtgcag gccgccgaga cgggcgaact
      721 gggcgtcctg caggcccggg atcatgcgga agacctcggc ctgggcgccg tgcttcagct
      781 tggtctggaa gccgaccatg ttgaacaggg tgcccagcgc attgtcctga cgcagctggg
      841 cgatggcgta tgacttgacc tgcgggtcgc gggggttggt caggccaacg ggcttcatcg
      901 gaccgtggcg aagggtctcg cggccgcgtt cggccatgac ctcgatgggc aggcaaccgt
      961 cgaaataggg gacgttctcc cagtccttga actcggcctt ggggccgttc agcagggcgt
     1021 cgatgaaggc ctcgtactgt tcccggttca tggggcagtt gacgtaggcg gcggcgtcgc
     1081 cgccgggacc ttccttgtcg tagcgcgact ggcgccaggc gatgtcgaag tcgatgctgt
     1141 cggcgtggac gatgggggcg atggcgtcga agaagctgag gctctcctcg ccggtcgtct
     1201 cgaggatggc ggcggccagg gcgggggagg tcagggggcc ggtggcgacg atgacgttgt
     1261 cccacgcctc gggcggcagg ccggcgatct cctcgcgcac gatggtgatc agggggtggg
     1321 cttccagctt ggccgtcacg gcctgcgaga agccgtcgcg gtcgacggcc agggcgccgc
     1381 cggcgggcac ctgatgggcg tcgccgcaac tcatgatgat ggaatcaagc gcccgcatct
     1441 cggcgtgcag caggccgacg gcgttgtatt cccagtcgtc cgatcggaac gagttggagc
     1501 agaccagttc ggccaggccg tcggtctggt gggcgtcggt gccgcggacc ggccgcatct
     1561 cgtgcaggat cacgggcacg ccggcctggg cgatctgcca ggtggcttcc gatccggcga
     1621 ggccgccgcc gacgacatgg atgggttcga tggtgctcat gccctgtgag taggcatggg
     1681 acgggcggag atcaacccgt ggaccgcctg gggagagccc aggaccatgg cggggcccga
     1741 aaacgtcgaa cgcccgccgg
//
LOCUS       DASVVN010000252         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_379275,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000252 DASVVN010000000
VERSION     DASVVN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379275"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..361
                     /locus_tag="VF686_03965"
     CDS             56..361
                     /locus_tag="VF686_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX8661000.1"
                     /translation="MLARIYRPAKTAMQSGKASAKGWRLEFEPASARTIDPLMGWTSS
                     TDMNGQVRLTFDSAEEAVAYAERYGIPFRLQEPQESPVILKAYADNFATFRKVPWTH"
     gene            446..522
                     /locus_tag="VF686_03970"
     tRNA            446..522
                     /locus_tag="VF686_03970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Arg,seq:tct)
     gene            639..1409
                     /locus_tag="VF686_03975"
     CDS             639..1409
                     /locus_tag="VF686_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8661001.1"
                     /translation="MRIATFNINGVNTRLPNLLAWLEEARPDVACLQELKATDAQFPA
                     KAIRDAGYHAVWKGEHRWNGVAILSRSEAVVTRRGLPGDDGDAQSRYIEAAVDGVLVG
                     CLYLPNGNPQPGPKFDYKLAWFERLIAHAGDLLTSGAPVVLAGDYNVVPTEADIYNSR
                     SWKKDALLQPESREAYARLLEQGWTDAIRERFPHDPPPWTFWQYFRQAWERDAGLRID
                     HLLLSPGVAARLSDAGVDREVRGRERASDHAPTWIVLD"
     gene            1440..1904
                     /locus_tag="VF686_03980"
     CDS             1440..1904
                     /locus_tag="VF686_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661002.1"
                     /translation="MARALKVFSYSDGFHSWTVATTSRAKALEAWGVKRDLFKDGSAV
                     EAKEGPDKEAALKAPGELIERGLSVDIGKVEKTKAPKAAKKRGPSAKDVARVELLEEE
                     LADLDAAQSEETEALEARRAELEREAAALARSQSKAREALKAKLKAARAKLK"
     gene            complement(1897..3069)
                     /gene="metC"
                     /locus_tag="VF686_03985"
     CDS             complement(1897..3069)
                     /gene="metC"
                     /locus_tag="VF686_03985"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX8661003.1"
                     /translation="MSDRSDRTRLIASATRRGRGRRAVAPPVERASTLLNDAPADMRD
                     GTLGPVYGIEDLSAARELRAALADLEGGTDAFLVPSGLAAVTVALTALLRPNDEVVTT
                     DALYGPTRRFLMRHMKARGVTTVFHAPDASTEDILACLTDQTRVLLIESPGSLTFEML
                     DVRALAAACRARGVRTVIDNTWAAGLAFRPLAHGVDVSVQAVTKYVGGHSDLLMGSIS
                     VADASLGRAIHETIEDVGWRVSADDAWLALRGIRTLPLRYEEQARSAMAVATWLQSRP
                     EIARVLYPALPGAVGHDLWRRDYTGAASLFGVVMQGGDEAAAHALMSTLELFGLGYSW
                     GGFESLITHETGQLSGRRNPPALPGQLLRLHVGLEAPADLIADLEAGLAAWRAAVT"
BASE COUNT          537 a          992 c         1309 g          461 t
ORIGIN      
        1 acaagctatt tgagccggac gcggggccag gcccccatac ttccaggacg cccccatgct
       61 tgcccgcata taccgccccg caaagaccgc gatgcagtcc ggcaaggcct cggcgaaagg
      121 gtggcgtctg gagttcgagc cggcgtcggc gcggacgatc gatccgctga tgggctggac
      181 cagttcgacc gacatgaacg gccaggtgcg cctgaccttc gactcggccg aggaggcggt
      241 ggcctatgcg gagcgatacg gcatcccgtt ccgcctgcag gagccgcagg agtcgccggt
      301 gatcctcaag gcctatgccg acaatttcgc gacgttccgc aaggttccgt ggacgcattg
      361 attcttgttg cggctaccgc gcttcgcgct acttgagccg cgctgttgtt cggctaccgc
      421 gcttcgcgct acttgagccg tccgggcgcc catagctcaa ccggatagag cacccgcctt
      481 ctaagcggga tgttgcaggt tcgagtcctg ctgggcgcgc cattcctgaa cccgctcaac
      541 cccatgaggc gcccgcttgg aaggcgggat gttgcaggtt cgagtcctgc tgggcgcgcc
      601 attcctgaac cacgccgccg cccgcgcatt cagcggcgat gcggatcgcg accttcaaca
      661 tcaatggcgt caatacgcgg ctgcccaacc tgctcgcgtg gctggaggag gcgcggccgg
      721 atgtcgcctg tttgcaggag ctgaaggcga cggacgcgca gtttccggcc aaggccatcc
      781 gcgacgcggg atatcacgcc gtgtggaagg gcgagcatcg ctggaacgga gtggcgatcc
      841 tgtcgcggtc cgaggcggtg gtgacccggc gcggcctgcc gggcgacgac ggcgacgcgc
      901 agagccggta tatcgaggcg gcggtggacg gggtgctggt cggctgcctg tatctgccca
      961 acggcaatcc gcagccgggg ccgaagttcg actacaagct ggcgtggttc gaacggctga
     1021 tcgcccatgc cggggacctg ttgaccagcg gcgcgccggt ggtgctggcg ggcgactata
     1081 acgtcgtgcc gaccgaggcg gacatctaca acagccgttc gtggaagaag gacgccctgc
     1141 tgcagcccga gagccgggag gcctatgcgc gattgctgga gcagggctgg accgacgcca
     1201 tccgcgagcg gtttccgcat gatccgccgc cgtggacctt ctggcaatat ttccggcagg
     1261 cgtgggagcg ggacgcgggg ctgcggatcg atcacctgct gctgagcccg ggcgtggcgg
     1321 cgcggctcag cgacgcgggg gtcgatcgcg aggtgcgcgg gcgggagcgg gccagcgacc
     1381 atgcgccgac gtggatcgtg ctggactgaa cccgcgcgca gcgtggccgt tcaggaggca
     1441 tggcgcgcgc tttgaaggtc ttctcgtatt ccgacggctt ccattcgtgg accgtggcga
     1501 cgacgtcgcg ggccaaggcg ctggaagcct ggggcgtgaa gcgcgacctg ttcaaggacg
     1561 gctcggcggt cgaggcgaag gaggggccgg acaaggaggc cgcgctgaag gccccgggcg
     1621 agctgatcga acgcgggctg tcggtcgata tcggcaaggt ggagaaaacg aaggcgccga
     1681 aggcggcgaa gaagcgcggg ccgagcgcga aggatgtggc gcgggtggag ctgctggagg
     1741 aggagctggc ggacctggac gccgcgcagt cggaggagac ggaggcgctg gaggcgcggc
     1801 gggcggagct ggagcgagag gcggcggcgc tggcgagaag tcagtcgaag gcgcgcgagg
     1861 ccctgaaggc gaagctgaag gcggcgcggg cgaagctcaa gtgaccgcag cgcgccaggc
     1921 ggcgaggccg gcttcgaggt cggcgatcag gtcggcaggg gcttcgaggc cgacgtggag
     1981 gcgcagcaac tggccgggca gggcgggcgg attgcggcgg ccggacagct ggccggtctc
     2041 gtgggtgatc aggctttcga agccgcccca tgaatagccg agaccgaaca gttccagcgt
     2101 cgacatcagg gcgtgggcgg cggcctcgtc gccgccctgc atgacgacgc cgaacaggga
     2161 ggcggcgccc gtgtagtcgc ggcgccaaag gtcatggccg accgcgccgg gcagggcggg
     2221 atagaggacg cgggcgatct cggggcggga ctggagccag gtcgccacgg ccatcgccga
     2281 gcgggcctgt tcctcgtagc ggaggggaag ggtgcggatg ccgcgcaggg ccagccaggc
     2341 gtcgtcggcg gagacgcgcc agccgacatc ctcgatggtt tcgtggatgg cgcggccgag
     2401 gctcgcgtcc gccacggaga tggagcccat gagcagatcg gaatggccgc cgacgtattt
     2461 ggtcaccgcc tgaacgctga cgtcgacgcc atgggccagc gggcggaagg cgagaccggc
     2521 agcccaggtg ttgtcaatga cggtgcgcac gccccgggcg cggcaggcgg cggcgagggc
     2581 gcgaacgtcc agcatttcga acgtcagtga gccgggcgat tcgatcagca ggacgcgggt
     2641 ctggtcggtc aggcaggcga ggatgtcctc ggtcgaggcg tctggcgcat ggaagacggt
     2701 cgtgacgccc cgcgccttca tatggcgcat caggaagcgg cgggtcgggc cgtagagggc
     2761 gtcggtggtc accacctcgt cgttggggcg cagcagggcg gtcagggcga cggtgacggc
     2821 ggcgagaccc gaagggacga ggaaggcgtc ggtcccgcct tcgagatcgg ccagagccgc
     2881 gcggagctct cgagccgcgc tgaggtcctc gatgccatag acggggccga gcgttccatc
     2941 gcgcatgtct gcgggcgcgt cgttcagcag ggtggaggcc cgttcgacgg gaggggcgac
     3001 ggcccggcgg ccacggccgc gccgggtggc ggaggcgatc agtcgggtgc ggtcgctgcg
     3061 gtcggacatc gggggcgagg aaccggaggg ggggaagggt tgcagatcaa cttccaaagg
     3121 cctctaaagc gaggcggggc gcaaggtctg acaggcttcg gcgaggcggt atgagagcag
     3181 gttcgagggc gcggcccatg gcgatcgcgg cgatcgcgac ggcggttctg acgcttgccg
     3241 cctgcggccg gggcgaccgg caggccgcgg acaccgcaga cgccggggcc cagacgaac
//
LOCUS       DASVVN010000253         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_457145,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000253 DASVVN010000000
VERSION     DASVVN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457145"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..926
                     /locus_tag="VF686_03990"
     CDS             225..926
                     /locus_tag="VF686_03990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8661004.1"
                     /translation="MLVLHYTGMKTARDAIARLQDPEAKVSAHYVVDEDGAILRLVAE
                     ERRAWHAGKGVWQGETDCNAASIGIEIVNPGHEFGYRDFPEAQIDAVISLISDIRTRW
                     TIPDARIIGHSDLAPERKQDPGERFPWKRLASVGHGLWFEPAPERTAALGVPLGVGDE
                     GLGVVVLRAGLHRLGYGLTPGGDYDAATKLTVEAFQRHWRPSRVDGIADGETRATLMG
                     VLQLATAESVTGVLQ"
     gene            923..1441
                     /locus_tag="VF686_03995"
     CDS             923..1441
                     /locus_tag="VF686_03995"
                     /inference="COORDINATES: protein motif:HMM:NF042561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6678 family protein"
                     /protein_id="HEX8661005.1"
                     /translation="MTLRSLESGAELEKRRTANGEHYRNVVLPKLKVDIQAEMDRRGL
                     AAFMNDTRWTALVKAVHEELPFPPPFQIQDIRGPRQPLWASDDVDHWGGWSAESLEPV
                     LAIEWIRVVPRYLKHRGMLIDPEIVDCTDQFRNLVKRLRLPWREDDRGIWIYGYAPSD
                     PATLTWNLEAPT"
     gene            1448..1827
                     /gene="rnpB"
                     /locus_tag="VF686_04000"
     ncRNA           1448..1827
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF686_04000"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2175..>2558
                     /locus_tag="VF686_04005"
     CDS             2175..>2558
                     /locus_tag="VF686_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX8661006.1"
                     /translation="MFLSTYEKQLDGKRRLLIPQEFRTADNGAEHGVFCFFSMASDCL
                     EAGGDKLMAEYVARIEALPFGSDRRTALEETFYAGQRPLAYDGGGRITLPEHLCQDAG
                     LGEDVVIVGMGSRFQIWDRARWNARR"
BASE COUNT          414 a          831 c          889 g          424 t
ORIGIN      
        1 cgccctgttc gcggccgtgc tcgtcttctt cgccggtctc gccctgggcg cacgccgcaa
       61 ggtcaccgtc cgcgcccgtc gcaccgacga cgtcatcgag gcccgcaagg tcgacggcgc
      121 ctgggtcgcc tacggctggg agcggccggg ccggtaagcg cgccgtgctg accgtcaagg
      181 acgcgccgtc gcccagtttc gacgcccgcc gcggccctcc cgacatgctg gtgctgcact
      241 acaccggcat gaagacggcg cgtgacgcca ttgcgcggct gcaggatccc gaggcgaagg
      301 tctcggccca ctatgtggtc gacgaggacg gcgcgatcct gcgcctcgtg gccgaggaac
      361 gccgcgcctg gcatgcgggc aagggcgtct ggcagggcga gaccgactgc aacgccgcct
      421 ccatcggcat cgagatcgtc aatcccggcc acgagttcgg ctatcgcgac tttcccgagg
      481 cgcagatcga tgcggtgatc agcttgatca gcgacattcg cacccgctgg accatcccag
      541 acgcccgcat catcggccat tcggatctcg cgcccgagcg gaaacaggat ccgggcgaac
      601 gcttcccgtg gaagcggctg gcctcggttg gccacggcct gtggttcgag ccggcgccgg
      661 aacggacggc ggcgctgggc gtgccgctgg gcgtcggcga cgaggggctg ggcgtggtcg
      721 tgctgcgcgc cggtctgcat cggctgggct acggcctgac gccgggcgga gactatgacg
      781 cggcgacgaa gctgaccgtc gaggccttcc agcgccactg gcggccgtcg cgtgtggacg
      841 ggatcgccga cggcgagacg cgggcgacgc tgatgggggt gcttcagctg gcgacggcgg
      901 agagcgtgac cggggtgctg cagtgactct tcggtctctg gaaagcggag ccgagcttga
      961 gaagcgccgc accgccaatg gggagcacta ccgcaacgtt gtgcttccga aactcaaggt
     1021 ggacattcag gcagagatgg accggcgggg gctcgccgcc ttcatgaatg acacgcggtg
     1081 gacggcgctc gtcaaggcgg tgcacgagga attgcctttt ccaccgccgt tccagatcca
     1141 ggacatcagg gggccgcgac agccgctgtg ggccagtgat gacgttgacc actggggcgg
     1201 ctggagcgcc gagagcctgg aacccgttct cgccatcgag tggattcggg ttgttccccg
     1261 ctacctcaag caccggggca tgctgatcga cccggagatt gtcgactgca ctgaccagtt
     1321 ccgtaatctg gtgaagcgcc tgcgcctgcc ttggcgcgaa gatgatcgcg gcatctggat
     1381 ctatggttat gccccgtctg atcccgcgac attgacctgg aacctcgaag cgcccaccta
     1441 ggccccgtca gacggccggg cggtcgcggc gggggttatg ccctcgtcga ggaaagtccg
     1501 ggctccacgg tgaaaaggcg gcgggtaact cccgcccggg gcgacccgag ggatagcgcc
     1561 acagaaagca aaccgcctgc ctctcggggc agggaagggt gaaagggtgg ggtaagagcc
     1621 caccgcgtgc ctggcaacag ggacggcatg gcaagccccg cctggagcaa gaccgaatag
     1681 ggacgtcgcg cgggttcgcc cgcagggttc atcgcccgag gcgtccgggt aggtcgcgag
     1741 aaccgctcag caatgggtgg tccagaggaa tgatcgtcac cgtccttcgg ggcggtacag
     1801 aacccggctt acaggccgtc tgacacactc tttttggggg cctacgctcg cgacgcggtc
     1861 gctcgctgct tgaggccggc cccctccgtc acggcgcgaa gatgcgccgc gccacctccc
     1921 ccggtggggg aggagatcgc tgcttggtga ttaacccctt ggcccggatt gcggcgcagg
     1981 atcagtccac aggctgtcta tactttgcgt gttctgtgta tgttccgtcc gttacagggc
     2041 tttcggcgcc tgtggaagtt aacttccgtc aactaatccc attgaggccc agttgatccc
     2101 atgctatccc attctctctg agggtgttca cgggcagagg ggcggatcgc gaggtccgag
     2161 ttgggggcaa gggcgtgttt ctctcgacct atgagaaaca gttggacggc aagcgacgcc
     2221 tgctgatccc gcaggagttc cgcacagccg acaacggcgc cgagcacggc gtcttctgct
     2281 tcttctccat ggcgtccgac tgcctcgagg ccggcggcga caagctgatg gccgaatacg
     2341 tcgcccgcat cgaagccctg ccgttcggca gcgatcggcg cactgctctc gaggaaacct
     2401 tctacgccgg ccagcgcccg ctggcctacg acggcggcgg ccgcatcacc ctgccggaac
     2461 acctgtgcca ggacgccggc ctcggcgagg acgtggtcat cgtcgggatg ggatcgcgct
     2521 tccagatctg ggaccgggcg cgctggaacg cccgccgc
//
LOCUS       DASVVN010000254         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_352525,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000254 DASVVN010000000
VERSION     DASVVN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352525"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VF686_04010"
     CDS             <1..746
                     /locus_tag="VF686_04010"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8661007.1"
                     /translation="DGTRVEAVQVIFRASPTYDGTMHYGSSLAFHPDGKLFITLGERS
                     VTEMRPQAQDLGSHMGKTIRINADGSVPQDNPFVGREGALPEIWSLGHRNVQGVAIAP
                     NGDVWTVEHGTRGGDEINLDRAGLNYGWPEVAYGIEYRGGPINGGVTAREGIEQPVYY
                     WDPVIAPGGMTIYDGAMFPGWRGNLLVAGLKEKHIARLVLEGGRVVGEERLMTDLGER
                     VRDVAVAADGSVWAITDEQNGKLVRLAAS"
     gene            complement(791..1651)
                     /gene="dapD"
                     /locus_tag="VF686_04015"
     CDS             complement(791..1651)
                     /gene="dapD"
                     /locus_tag="VF686_04015"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX8661008.1"
                     /translation="MLDRAHLESVIEAAWEDRAGISVSTHGEVRKAVEQALAMLDSGE
                     ARVASRGDDGVWTTHQWLKKAVLLSFRLNPNTRMGAPVGATMGVTDPTPGPYWDKVPV
                     KWAHWTEADFERAGFRAVPGAVARQGAFIGRNVVLMPSFVNIGAYVDEGSMVDAWATV
                     GSCAQIGKNVHLSGGAGIGGVLEPLQANPTIIEDGCFIGARAEVAEGVIVREGAVLAM
                     GVYLSGSTKIVDRATREVFRGEVPAYSVVVPGSLPDPAGGPSLYCAVIVKRVDAQTRA
                     KTGVNELLRD"
     gene            complement(1651..2343)
                     /locus_tag="VF686_04020"
     CDS             complement(1651..2343)
                     /locus_tag="VF686_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX8661009.1"
                     /translation="MSAGAGEATETGADLRHIRSWIFDLDDTLYPPESRFMGLIQQRI
                     NDYVVRTSGLPADEALVIQKAYLNDYGTSLAGLMAHYRIDPHDFLAEVHDVPLDVLTP
                     DPGLRAALERLDGPRIVFTNGSKAHARRVIERLELGDLFDGLFSLEDADLVPKPDPRT
                     FARMTEQFGVAPASAAFFEDTVRNLAPAHALGMTTVLVGTRAHDADIPFVDHRAASLG
                     PFLATARLPGDA"
     gene            complement(2340..>2566)
                     /locus_tag="VF686_04025"
     CDS             complement(2340..>2566)
                     /locus_tag="VF686_04025"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168728.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX8661010.1"
                     /translation="GADGQILRQMTVGEASKLIDDGVATGGMIPKLQTAIAAVRDGVE
                     AVVILDGRRPHAMLVELFTEHGAGTLVKAE"
BASE COUNT          401 a          896 c          891 g          378 t
ORIGIN      
        1 acgacggaac gcgggtcgag gccgttcagg tcatcttccg ggcctccccg acctatgacg
       61 ggaccatgca ctacggctcc tccctcgcct tccatccgga cggcaagctg ttcatcaccc
      121 tcggcgagcg ctcggtgacg gagatgcggc cacaggcgca ggatctcggc tcgcacatgg
      181 gcaagaccat ccgcatcaac gccgacggct ctgtcccgca agacaatccc ttcgtcggcc
      241 gcgagggggc gttgccggaa atctggtcgc tggggcatcg caacgtgcag ggcgtcgcca
      301 tcgcccccaa cggcgacgtc tggacggtgg agcacggcac gcggggcggc gacgagatca
      361 atctggaccg cgcgggcctg aactacggct ggcccgaggt cgcctacgga atcgagtacc
      421 gcggcggtcc gatcaacggc ggcgtcacgg cgcgggaggg gatcgaacag ccggtctact
      481 actgggaccc cgtcatcgcg ccgggcggca tgaccatcta cgacggggcc atgttccccg
      541 gctggcgcgg caatctgctg gtcgccgggc tgaaggaaaa acacatcgcc cggctggtgc
      601 tcgagggcgg ccgggtcgtc ggcgaggagc ggctgatgac cgatctgggc gaacgggtcc
      661 gcgacgtggc cgtggccgcc gacggctcgg tctgggccat caccgacgag cagaacggca
      721 agctggtgcg gctcgccgcc tcttgagggc gctaccgccc ggcgcgcggc cccattgcct
      781 gagtgcggcg tcagtcccgc aacagctcgt tgacgcccgt cttggcccgg gtctgcgcgt
      841 cgacccgctt gacgatgacg gcgcaataga gcgacggccc gcccgcggga tcgggcaggg
      901 agcccggcac cacgacgctg tacgccggca cctcgccccg gaagacctcg cgggtcgccc
      961 gatcgacgat cttcgtcgag ccggacaggt agacgcccat ggccaggacc gcgccctcgc
     1021 gcacgatcac gccctcggcg acctcggcgc gggcgccgat gaaacagcca tcctcgatga
     1081 tggtcggatt ggcctgcagc ggctccagca cgccgccgat gcccgccccg cccgacaggt
     1141 gcacattctt gccgatctgg gcgcaggaac cgaccgtggc ccaggcgtcg accatcgaac
     1201 cctcgtcgac ataggccccg atgttcacga agctgggcat cagcacgacg ttgcggccga
     1261 tgaaggcgcc ctgacgcgcc acggcgcccg gtacggcgcg gaagccggcc cgctcgaaat
     1321 cggcctcggt ccagtgggcc catttgaccg gcaccttgtc ccagtacggc cccggcgtcg
     1381 ggtccgtgac gcccatggtc gccccgaccg gagcgcccat gcgggtgttg gggttgagcc
     1441 ggaacgacag cagcaccgcc ttcttcagcc actgatgcgt ggtccagacg ccgtcgtccc
     1501 cgcgcgacgc gacgcgggcc tcgcccgagt ccagcattgc cagcgcctgc tccacggcct
     1561 tgcggacctc gccgtgggtc gagaccgaaa tcccggcccg gtcctcccag gcggcttcga
     1621 tgacagattc aaggtgcgcg cgatccagca tcaggcgtct ccgggcaggc gggcggtggc
     1681 gaggaagggg ccgagcgaag cggcccggtg gtcgacgaac ggaatgtcgg cgtcgtgggc
     1741 ccgggtcccg accaggacgg tcgtcatgcc gagggcgtgg gccggcgcga ggttccgcac
     1801 cgtatcctcg aaaaaggcgg ccgaggccgg cgcgacgccg aactgttcgg tcatccgggc
     1861 gaaggtgcgc gggtcgggct tggggaccag gtcggcgtct tccagcgaga acaggccgtc
     1921 gaacaggtcg cccagctcca gccgctcgat cacccgccgg gcgtgtgcct tcgatccgtt
     1981 ggtgaagacg atgcgcggcc cgtccagccg ctccagcgcc gcgcgcaggc cggggtcggg
     2041 cgtcagcacg tccagaggca cgtcgtgcac ctcggccagg aagtcgtgcg gatcgatgcg
     2101 gtaatgggcc atcagcccgg ccagcgaggt gccgtagtcg ttcagatagg ctttctgaat
     2161 cacaagggct tcgtctgccg gcaggcccga ggtgcgcacc acatagtcgt tgatgcgctg
     2221 ctggatcagc cccatgaacc gggactcggg cggatacagg gtgtcgtcca gatcgaagat
     2281 ccagctacga atgtggcgca ggtctgcgcc cgtttccgtc gcctcgccgg ccccagcgct
     2341 cattccgcct tcaccagtgt gcctgcgccg tgttcagtga acagttcgac cagcatggcg
     2401 tgggggcggc gtccatcgag gatgaccacg gcctcgaccc cgtcgcgcac ggccgcgatg
     2461 gccgtctgca gcttggggat catgccgccg gtggccacgc cgtcatcgat cagcttcgag
     2521 gcctcgccca cggtcatctg gcggagaatc tggccgtccg cgccct
//
LOCUS       DASVVN010000255         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_379464,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000255 DASVVN010000000
VERSION     DASVVN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379464"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF686_04030"
     CDS             complement(<1..546)
                     /locus_tag="VF686_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661011.1"
                     /translation="MTGPVIESLAGSADLPAGSAESESFVVHQIVCPSPSARVLGLRR
                     RMGPGPAVHAPIADHRIIVHASAATWSACRASGASHLRRRGDIDLIPGGEEGGFDAQA
                     SWEELEIRIAPELVGDAGEAAAAAFEMKHMLRDERVLHLVRSLCDEDGDFALDDVLYA
                     ESVGAALLRRLAAPGVAARQDP"
     gene            576..1481
                     /locus_tag="VF686_04035"
     CDS             576..1481
                     /locus_tag="VF686_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8661012.1"
                     /translation="MVLDVVVGAGPVGRETARLLAASGSEVRLVSRTGNAPPAPGISG
                     VSLDGRDASALAEISRGARTIHMCAMAPYTSWPTDFPPIMEGVAGAAERVGARLIVLG
                     NVYGYGQAATSPLKASSPLAPTSVKGEVRTRMWERARNGPAPAIEVRGSDYLGRGAGS
                     LFTLMALPSILAGREVVLPADLDAVHAWTFTGDAARTLVAAAAHEGEWNRAFHVPSHH
                     LSIRDLTARFCDLAGVKRTTLRALTRAELEAQAAADPFMREIAEMAYLFYEPCVLDPS
                     DTERLLGVGRSPLDAIISDTLAGAG"
     gene            1661..2128
                     /locus_tag="VF686_04040"
     CDS             1661..2128
                     /locus_tag="VF686_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8661013.1"
                     /translation="MRVRPFKPDDAARLVALFSDPRVARFVGDGLPLSPDQAQAWVRT
                     SAANLERFGYGTGAVIDKAGGPLIGWAGVARPPGDEEEIIYGLEASHWGQGYGRELVE
                     ALVRFATAQGLTPVRATVDRLNAGSVRLLVVNGFRPAIRDYQGEAGTDLYLRP"
BASE COUNT          313 a          739 c          775 g          328 t
ORIGIN      
        1 cgggtcctgc cgggcggcta cgccgggggc ggcgagccgc ctcagcaggg cggcgccgac
       61 gctttccgca taaaggacgt cgtccagggc gaagtcgcca tcctcgtcgc acagcgatcg
      121 cacgaggtgg aggacccgtt catccctgag catgtgtttc atctcgaaag cggcggcggc
      181 cgcttccccc gcatctccga ccagttcagg ggcgatgcgg atttccagct cctcccacga
      241 cgcttgggcg tcgaaaccgc cctcctcgcc cccggggatg aggtcgatgt cgccccgccg
      301 ccgcagatgg ctcgcgccgg aggcccggca ggcggaccag gtcgcggcgc tcgcgtgcac
      361 gataatccga tgatcggcga tcggcgcgtg gacggccgga cccggcccca tcctgcggcg
      421 caggccgagc acacgcgccg acggcgaggg gcagacgatc tgatgcacga cgaagctttc
      481 cgattcagca gaacctgccg ggagatcggc gcttcctgcc agactttcaa tgaccggacc
      541 ggtcacgctc gggacttcaa ttcgggagaa ggttcatggt gctggatgtg gttgtcggcg
      601 cggggccggt aggacgtgag acggcgcggc tgctggcggc gtcagggagc gaggtgcggc
      661 tcgtcagccg gaccggcaat gcgccgccgg cgcccgggat cagcggcgtg agcctggatg
      721 gtcgggacgc ctcagcactg gccgagatca gccggggcgc ccgcaccatc catatgtgcg
      781 cgatggcgcc ctatacgagc tggccgaccg attttccccc gatcatggag ggtgtcgcgg
      841 gcgcggccga aagggtcgga gcccggttga tcgtgctcgg caatgtctac ggctacggac
      901 aggcggcgac ctcgcccctg aaggcctcct cgcccctggc gccgaccagc gtcaagggcg
      961 aggtccggac gaggatgtgg gaacgggccc ggaacggccc ggcgcccgcc atcgaagtcc
     1021 ggggcagcga ctacctgggc cgaggcgccg ggtctctgtt caccctaatg gcgctgccgt
     1081 cgatcctggc gggccgggag gtcgtcctgc cggccgacct ggatgccgtt cacgcgtgga
     1141 ccttcacggg ggacgcggcg cgaaccctcg tcgcggcggc ggcccatgaa ggcgaatgga
     1201 accgggcctt ccatgtgccc tcccaccatc tgtcgatccg cgatctcacc gcccgcttct
     1261 gcgatctcgc cggggtcaag cgcacgacgc tgcgcgccct gacgcgcgcc gaactcgaag
     1321 cgcaggcggc agccgatccg ttcatgcgcg agatcgcgga aatggcctac ctcttctacg
     1381 agccctgcgt cctggacccc tccgacaccg aacgtcttct cggcgtcggc cgcagtccgc
     1441 tcgacgcgat catctcggac accttggccg gcgccggctg acggcggatt cgaggatcgg
     1501 tcggaccctg cagcggacgc cagatgcggg agagcgccga tctggacacc ccgggagcct
     1561 cgtcctacgg gtcggccgcg cgaagcttcg cgagccggcg tgaccgctgg gagccgacgg
     1621 agttcgcatg atcctgaagg gcatcctgta aaccccgcgt ctgcgggtcc gcccgttcaa
     1681 accggacgac gcggcacgcc tggtcgccct gttctcggac ccgcgggtgg cgcgcttcgt
     1741 cggcgacggc ctgccccttt cgccggatca ggcgcaggcc tgggtccgga cgtcggcggc
     1801 gaacctggag cgcttcggct acggcacggg ggcggtgatc gacaaggccg gcggtccgct
     1861 gatcggctgg gccggcgtgg cgcgaccgcc gggagatgaa gaagagatca tctacggcct
     1921 ggaggcctcg cattgggggc agggctacgg gcgtgaactg gtcgaggccc tcgtccgctt
     1981 cgcgaccgcg cagggcctga ctcccgttcg cgcgaccgtg gacaggctga acgccggctc
     2041 tgtgcgcctg ctggtcgtga acggctttcg gccggcgatt cgggactatc agggtgaggc
     2101 ggggacggac ctttatcttc ggccctgacc ctggacgggc cgtgcggcga ccctc
//
LOCUS       DASVVN010000256         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_114034,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000256 DASVVN010000000
VERSION     DASVVN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114034"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VF686_04045"
     CDS             <1..461
                     /locus_tag="VF686_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847588.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX8661014.1"
                     /translation="RNLAGYPKASSTEFGPTIEPVVGLMTEWTQGNQRVLRQQGGDMG
                     GTMRLGAYDSTLKPGSKIAHMYGSTTISERHRHRYEVNINYREAIEQKGGLTFAGLSP
                     DGVLPETIEREDHPWFIGVQYHPELKSRPFAPHPLFAGFIEAAVVQSRLV"
     gene            complement(668..1567)
                     /locus_tag="VF686_04050"
     CDS             complement(668..1567)
                     /locus_tag="VF686_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8661015.1"
                     /translation="MADFNWNDLRAFLAVARTGRLTAAAARVAMDHTTVARRIAALEA
                     SLGARLFDRSPHGYALTPHGERLMPTAEKIESLTIRAADELGEADQALTGTVRIGAPE
                     GFGSYVLAPLMARLADRHPGLDIQLVAISGVLSLSKREADIAVTLSAPREGRLVSRKL
                     TDYGLSLYAAPSYLDARPPILTRADMAGHRFIGYIGDLLYAPELDYMQAPDVDIHVAL
                     QSSNLIAQLQAALAGAGLCVLPDFIASREAGLRRVLHDAVHLDRSLWLVTHADLRPLA
                     RIRAVTAMILEAVKADRIMFTGT"
BASE COUNT          327 a          594 c          597 g          297 t
ORIGIN      
        1 cgcgaaatct ggccgggtat ccgaaggcct catccaccga gttcgggcct acgatcgagc
       61 cggtggtcgg cctgatgacc gaatggaccc agggcaatca gcgcgtcctg cgccagcagg
      121 gcggcgacat gggcggcacc atgcggctgg gcgcctatga ttcgacgctg aagcccggat
      181 cgaagatcgc gcatatgtac ggctcgacca ccatcagcga gcggcaccgc caccgctatg
      241 aggtcaacat caactatcgc gaggcgatcg agcagaaggg cggcctgacg ttcgccggcc
      301 tgtcgcccga cggcgtcctg ccggaaacga tcgagcgcga ggaccatccg tggttcatcg
      361 gcgtgcagta tcatcccgag ctgaagagcc ggcccttcgc gccccatccg ctattcgcag
      421 ggtttattga agcggccgtt gtgcagagtc gattggtttg acgggaggga taacgccctc
      481 gaacggccgg cgatgccggc atcggacgca cacttcggta cccggctgac tcctgctgtc
      541 gacgtagatg tggcccttgc gggcgcattt cagtgactcg aacacagccg cttcccttcg
      601 cctgtttctt caaagaacga tgttctgttg attgttgcag tgcaggaatc gaacgggaaa
      661 accggtctca ggttcccgtg aacatgatcc ggtccgcctt gaccgcctca aggatcatgg
      721 cggtcacggc ccggatgcgg gccagcggcc gcagatccgc atgggtcacc agccacaggc
      781 tgcggtccag atgcacggcg tcgtgaagga cgcggcggag gccggcctca cgcgaggcga
      841 tgaagtccgg cagcacgcaa agtcccgccc ctgccagggc ggcctgcaac tgagcgatca
      901 gatttgagct ctgcagggcg acgtgaatgt cgacatcggg cgcctgcata tagtccagct
      961 cgggcgcata gagcagatcg ccgatgtagc cgatgaagcg gtgtcccgcc atgtcggccc
     1021 gggtcaggat cggcgggcgg gcgtccagat aggacggcgc ggcatacagg ctcaggccgt
     1081 agtcggtcag tttgcgcgac accagccggc cctcgcgcgg cgcgctgagc gtcaccgcga
     1141 tatcggcttc gcgcttggac aggctgagca cgccggagat ggccaccagc tggatatcca
     1201 gtcccgggtg ccggtcggcc aggcgggcca tcagcggggc cagcacgtag ctgccgaagc
     1261 cctcgggcgc gccgatccgg accgtgccgg tcagcgcctg gtcggcctcg cccaattcgt
     1321 cggcggcccg aatggtcagg ctctcgatct tctcggcggt cggcatcaga cgttcgccat
     1381 gaggcgtgag ggcgtagccg tggggactgc gatcgaacag ccgggcaccc agactggcct
     1441 ccagcgcagc gatgcgccgg gccaccgtgg tgtggtccat ggccacgcgc gccgccgcag
     1501 cggtcagtcg ccccgtccgg gctacggcca gaaaggcccg tagatcattc caattaaagt
     1561 ccgccatccg ggctcctgtg caaaaatgca caactattgc tcaaaccttg gcatcgtcag
     1621 aacagaaaag ccatgagata ccgccgctat cggacgcgac cggttcagag gactcccgcc
     1681 atggcattcg acgccgccgc ctacacttcc aactcgctga agccgcggac tccggtcccg
     1741 gcgccgatcg cggctacgga cgagctttgc ccggtctggg cggccgagaa cccggaccgc
     1801 gcccttgcgt tgctg
//
LOCUS       DASVVN010000257         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_298939,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000257 DASVVN010000000
VERSION     DASVVN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298939"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1689
                     /locus_tag="VF686_04055"
     CDS             340..1689
                     /locus_tag="VF686_04055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8661016.1"
                     /translation="MRTLILAAALIAVSPFAAFAQTPAPAAPSASVRAMAAGYKALTV
                     CSALKTAEAAGGTRTLESVEANELTGIYPELDALVREMPVEVSAGGTSVAWDEAMPPR
                     RAVWSPGRGCAIMPVGWRGDEIAIRPPGRPSDQTFATGEPAGNVALLNEAVEQAFEGG
                     YGEGSNTTAVLVLQNDRVVAEEYALGFGPDTPQRTWSVAKSLAGTVIGAAVRRGEADV
                     NAPAAIADWSRDGDPRAAITLDQLMRMASGLISDTAGNRTDALYFGGVGVDEQVTTWP
                     LIAPPGTRFRYANNDILLSILAIAPTFEAHPPAELFRRLGMNDTWAETDWRGNYMLSS
                     QVWSTARDLARFGRLYLNDGMVDGQRVLPEGWREHVSRPSGPQPPTGEQGYGATFWLF
                     NKSDGIPADAISANGNRGQYVIIVPSRNIVIVRRGEDPAGKPFNFIPFTRDVLAALD"
     gene            1774..2025
                     /locus_tag="VF686_04060"
     CDS             1774..2025
                     /locus_tag="VF686_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX8661017.1"
                     /translation="MADPHHADSHDAYVRGSQEIGEQSSTFHAFIKMSKWGSLWIAAL
                     LMFLVLWFQPGGSFIGGAIAAVAMLVIGYFALKSGKKAH"
     gene            2165..>2862
                     /locus_tag="VF686_04065"
     CDS             2165..>2862
                     /locus_tag="VF686_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX8661018.1"
                     /translation="MAVAIAVTRERRDGETRCAVTPETVKKLIAMGATVTVEAGTGAG
                     SSIPDDEYAAAGATVAKDTKAVLSGADIVLKVRGPTAQETSALKPGAIVVALLDAYRD
                     KETVTALSQAGATAFAMEFVPRISRAQVMDALSSQANLAGYRAVIEAAWAYGKGFPMM
                     MTAAGTVAPAKVFVMGVGVAGLQAIATARRLGAVVTATDVRPATKEQVESLGAKFLAV
                     EDEEFKNAQTAGGYA"
BASE COUNT          442 a          936 c         1051 g          433 t
ORIGIN      
        1 cgcgggcggc gggtcgcggg gctatgtcga ggccttggcg ccgttcgggc tgaacccgcg
       61 cgatccagcg ttctggacca tcgggaccaa gcggctggaa gggctgatcg accagttcga
      121 ggcgctggtc tgagtccacc cctgatcccg tcattccggg gccgcgcagc ggaacccgga
      181 acccgggaga ccgcgatcag aaggttgaag cgcggtcggt gatagcatcc ggactcctgg
      241 gttccgggtt ctgtccccgg ctacgccggg cccagccccg gaatgacatg gaggagggca
      301 ggcattgacg cgcaccctga gccgggcgag cgtcgggtca tgcgcaccct gatcctcgcc
      361 gcggccctga tcgccgtctc tccgttcgcc gccttcgccc agaccccggc tcctgccgcg
      421 ccgtcagcga gcgttcgcgc catggcggcg ggctacaagg ccctcaccgt ctgcagcgcg
      481 ctgaagacgg ccgaggcggc gggcggaacg cggacgctgg aaagtgtcga ggccaatgag
      541 ctgacgggga tctatccgga gctggacgcg ctggtgcgcg agatgcccgt cgaggtgtcc
      601 gctggcggga cttcggtcgc gtgggacgag gcgatgccgc cgcgccgggc cgtatggtcg
      661 ccgggccggg gctgcgcgat catgccggtg ggctggcggg gcgatgagat cgccatccgg
      721 cctccgggac gtccttcaga ccagacgttc gcgaccggcg agccggcggg caacgtggcc
      781 ctgctgaacg aagcggtcga gcaagccttc gaaggcggct acggcgaggg ctccaacacc
      841 accgccgtgc tggttttgca gaacgaccgg gtggtggcgg aggaatacgc actgggcttc
      901 gggccggaca cgccgcagcg gacgtggtca gtggccaaat cgctggccgg cacggtgatc
      961 ggggcggcgg tccggcgcgg cgaggccgat gtgaacgcgc cggcggcgat cgccgactgg
     1021 agccgcgacg gcgacccgcg cgcggcgatc acgctggacc agctgatgcg gatggcctcg
     1081 ggcctgatca gcgacacggc gggcaaccgg acggacgcgc tgtatttcgg tggcgtgggc
     1141 gtggacgagc aggtcacgac ctggcccctg atcgcgccgc cgggaacgcg gttccgctat
     1201 gccaacaacg acatcctgct aagcattctg gccatcgccc cgacgttcga ggcgcatccg
     1261 ccggccgaac tgttccggcg cctgggcatg aatgacacct gggccgagac cgactggcgc
     1321 ggcaactaca tgctgtccag ccaggtctgg tcgacggcgc gcgatctggc gcggttcggg
     1381 cggctgtatc tgaacgacgg gatggtggac gggcagcgcg tcctgcccga gggctggcgc
     1441 gagcatgtct cgcggccgtc agggccgcag ccgccaaccg gggagcaggg ctatggcgcg
     1501 accttctggc tgttcaacaa gtcggatggg attccggccg acgccatctc ggccaacggc
     1561 aaccgcgggc aatatgtgat catcgtgccg agccggaaca tcgtcatcgt gcggcgcggc
     1621 gaggacccgg ccggaaagcc gttcaacttc atccccttca cccgcgacgt cttggcggcc
     1681 ctcgactgag gggaaatgtc agccgccgct tgagcggacg gcaggccccg ctataagggc
     1741 gccaaccgcc gttccagatt cccggacacc gacatggccg acccgcacca cgccgattcc
     1801 cacgacgcct atgttcgcgg ctcccaggag atcggcgagc agtcctcgac cttccacgcc
     1861 ttcatcaaga tgtcgaagtg gggttcgttg tggatcgcgg ccctgctgat gttcctggtg
     1921 ctgtggttcc agccgggcgg cagcttcatc ggcggcgcca tcgcggccgt ggcgatgctg
     1981 gttatcggct atttcgccct gaagtcgggc aagaaggcgc actgagtttt cggggcgcta
     2041 acgctcccga cgcggtcggt cgcttcttga gcgccgatct gacgccgcgc ctgcttcggg
     2101 cgctttgccg caacctcttc gatcagccta acgtcgtcct ccgagagatg acggagggat
     2161 tcgcttggct gtcgccatcg ccgtgacccg cgaacgccga gacggcgaga cgcgctgcgc
     2221 cgtgactccg gaaacggtga agaagctgat cgccatgggc gcgaccgtga cggtcgaggc
     2281 cgggacgggc gccggctcct cgatccccga cgacgaatac gccgccgccg gcgcgaccgt
     2341 ggccaaggac accaaggcgg tgctgtcagg cgcggacatc gtgctgaagg tgcgcggccc
     2401 gacggcgcag gagaccagcg cgctgaagcc cggcgcgatc gttgtggccc tgctggacgc
     2461 gtaccgcgac aaggagacgg tgacggcgct gtcgcaggcc ggtgcgaccg ccttcgccat
     2521 ggagttcgtg ccgcgcatct cgcgggccca ggtgatggac gccctgtcgt cgcaggcgaa
     2581 tcttgcggga taccgggcgg tgatcgaggc ggcctgggcc tatggcaagg gcttcccgat
     2641 gatgatgacg gcggcaggga cggtggcccc ggccaaggtc ttcgtcatgg gcgtgggcgt
     2701 ggcggggctg caggccatcg ccacggcgcg gcggcttggg gcggtggtca cggccaccga
     2761 cgtgcggccg gcgaccaagg agcaggtgga gtcgcttgga gcgaaattcc tcgccgtcga
     2821 ggacgaggag ttcaaaaacg cccagaccgc cggcggctac gc
//
LOCUS       DASVVN010000258         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_457431,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000258 DASVVN010000000
VERSION     DASVVN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457431"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /gene="nrdR"
                     /locus_tag="VF686_04070"
     CDS             complement(<1..363)
                     /gene="nrdR"
                     /locus_tag="VF686_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8661019.1"
                     /translation="MKCPFCGHQDTQVKDSRPSDDGAAIRRRRSCPQCSGRFTTFERV
                     QLRELMILKRNGRRTPFDRDKLERSLNVALRKRPVQAEQVEQLVSRITRQLESLGETE
                     IPSSTVGDFIMKALKSVDE"
     gene            complement(371..1654)
                     /gene="glyA"
                     /locus_tag="VF686_04075"
     CDS             complement(371..1654)
                     /gene="glyA"
                     /locus_tag="VF686_04075"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8661020.1"
                     /translation="MTAFTHADYFSKSLAEADPDIFGGIQGELGRQKEQIELIASENI
                     ASKAVLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVTEDLARSRAKQLFGCAFANVQPH
                     SGAQANQAVFQALLTPGDTFLGMDLAAGGHLTHGSPANQSGKWFRPVTYKVREDDCLI
                     DYDHVAEMAEKEKPKLILAGASAYSRHIDFKRFREIADSVGAYLMVDMAHYAGLIAGG
                     VYPDPLPHAHVVTTTTHKTLRGPRGGMVLSNDIELGKKINSAVFPGLQGGPLEHVIAA
                     KAVAFGEALKPEFKLYAKQVVTNAQALAAVLVERGAAIVSGGTDSHLMLVDLRPKGVT
                     GKATEAALEHALMTCNKNGVPFDTAPFTVTSGVRLGTPAGTTRGFGVGEFQQVGHWIA
                     DVIDSMKGTDEADAAVSQRVAGEVRELTRRFPIYG"
     gene            complement(1759..2154)
                     /locus_tag="VF686_04080"
     CDS             complement(1759..2154)
                     /locus_tag="VF686_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8661021.1"
                     /translation="MTLTAACHCGSTKITLPGPPTEAKTCNCSYCQRTGAVWGYYAPE
                     DLSVTAKEDRVYSASGLNQHHFCGACGGNTHGFSPDWASKYSSDGTLKPGEAEGVPER
                     KVAAVNLNMIDNLELSGLTVQAMDGRNSW"
     gene            complement(2221..2376)
                     /locus_tag="VF686_04085"
     CDS             complement(2221..2376)
                     /locus_tag="VF686_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661022.1"
                     /translation="MIVETTRSGLYLKCCAVHVATGVEAVAIGPAHEPKAVERLAIAK
                     LRRALER"
     gene            complement(2393..>3231)
                     /gene="hemA"
                     /locus_tag="VF686_04090"
     CDS             complement(2393..>3231)
                     /gene="hemA"
                     /locus_tag="VF686_04090"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX8661023.1"
                     /translation="PGLIVFSDELNHNSMISGIRTGPREQRRVFRHNDLAHLEDLLAA
                     APADAPKLIAFESVYSMDGDIADLAGTIALAKKYGAMTYLDEVHAVGLYGATGAGVAE
                     RDGLLDGIDIVECTLGKAIGVMGGYIAADAVIVDAVRSWASGFIFTTSLPPALTAGAL
                     ASVRHLKAHPELREAHQERAATLKARFAAAGIPVMESVSHIVPVFVGDPVHTKMISDM
                     LLEEHGVYVQPINYPTVPKGTERLRFTPSPNHTDAMMDDLVRAMDALWTHCNVARLPA
                     VA"
BASE COUNT          496 a         1106 c         1048 g          581 t
ORIGIN      
        1 ctcatcgacc gacttcagcg ccttcatgat gaagtcgccg acggtcgagg acgggatttc
       61 cgtctcgccc aggctttcca gctggcgggt gatgcggctg accagctgtt cgacctgttc
      121 ggcctgcacc ggccgcttgc gcagggccac gttcagcgac cgctccagct tgtcccggtc
      181 gaagggcgtg cggcggccgt tgcgcttcag gatcatcagc tcgcgcagct gcacccgttc
      241 gaaggtggtg aaacgcccgc tgcactgcgg gcaggaccgc cggcgtcgga tggccgcgcc
      301 gtcatccgac gggcggctgt ccttcacctg ggtatcctga tgtccgcaga aagggcattt
      361 catcagacgg ttatccgtat atcgggaagc ggcgcgtgag ctcgcgcacc tcgcccgcca
      421 cgcgttgcga cacggcggcg tccgcctcgt ccgtgccctt catcgagtcg atcacgtcgg
      481 cgatccagtg gccgacctgc tggaactcgc cgacgccgaa accccgcgtc gtgcctgccg
      541 gcgtgcccag acggacgccc gaagtcaccg taaacggcgc ggtgtcgaac ggcacgccgt
      601 tcttgttaca ggtcatcagg gcgtgctcga gagccgcctc cgtagccttg cccgtcacgc
      661 ccttgggccg caggtcgacc agcatcaggt ggctgtccgt gccgcccgag acgatggccg
      721 cgccgcgctc gaccagcacg gcggccagcg cctgcgcgtt ggtcaccacc tgtttcgcat
      781 agagcttgaa ctcgggcttc agcgcctcgc cgaaggccac ggccttggcg gcgatgacat
      841 gctccagcgg cccgccctgc agacccggga acaccgccga attgatcttc ttgcccagtt
      901 cgatgtcgtt cgacagcacc atgccgccgc gcggcccgcg cagggtcttg tgcgtcgtcg
      961 tggtcaccac gtgggcgtgc ggcaacggat cgggatagac gccgccggcg atcaggcccg
     1021 cataatgggc catgtccacc atcaggtatg cgcccacgct gtcggcaatc tcgcggaacc
     1081 gcttgaaatc gatatggcgg ctgtaggcgc tggcgccggc caggatcagc ttcggcttct
     1141 ccttctcggc catttccgcg acgtggtcat agtcgatcag gcagtcgtcc tcgcgcacct
     1201 tgtaggtcac gggccggaac cacttgccgg actggttggc gggcgagccg tgggtcaggt
     1261 ggccgccggc cgccagatcc atacccagga aggtgtcgcc gggcgtcagc agcgcctgga
     1321 acaccgcctg gttcgcctgt gcgcccgagt gcggctggac gttggcgaag gcgcagccga
     1381 acagctgttt cgcccggctg cgagccagat cctcggtgac atcgacatat tcgcagccgc
     1441 catagtagcg gcggcccgga tagccctcgg cgtatttgtt ggtcaggacc gagccctgcg
     1501 cctccagcac cgccttcgag gcgatgttct cggacgcgat cagttcgatc tgttccttct
     1561 gccggcccag ttcgccctgg attccgccga agatgtcggg atcggcctcg gcgaggctct
     1621 tcgagaaata gtcggcgtgg gtgaaggcgg tcactcggtt ccccgatgca ggcgtggcgg
     1681 tatggaaggc gacctatcta ggcccctgtt ccgccgacca caacatctgg ggtccggtcg
     1741 ccgcctcgcc ccgtccgcct accagctgtt ccgcccgtcc atcgcctgta cggtgaggcc
     1801 cgacagttcg agattgtcga tcatgttcag gttcaccgct gcaacctttc gctcgggaac
     1861 gccctccgcc tcccccggct tcagcgtgcc gtcgctgctg tatttggacg cccagtccgg
     1921 cgaaaagccg tgggtgttgc cgccgcacgc gccgcagaag tggtgctggt tcagcccgct
     1981 ggccgaatag acgcgatctt ccttcgcggt gacggacagg tcctcgggcg cataatagcc
     2041 ccacaccgcg ccggtgcgct ggcagtagct gcagttgcag gtcttggcct cggtcggagg
     2101 ccccggcagg gtgatcttcg tggatccaca gtgacaggcg gcggtcaggg tcatctcgca
     2161 actccgttgg ctggtccggc tgcctatcaa ccaccgatgt caggagttgt cagcagcctg
     2221 tcagcgttcc agcgcgcgcc tcaacttggc gatcgccaac ctttcgaccg ccttgggctc
     2281 atgcgcaggg ccgatcgcca ccgcctcgac gcccgtagcc acatgaacgg cgcaacattt
     2341 caggtagagg ccgctgcggg tggtctcgac aatcacctcg ccgaggctgt cgtcaggcga
     2401 cggcaggaag gcgcgccacg ttgcagtggg tccacagggc gtccatggcg cggaccagat
     2461 cgtccatcat ggcgtcggtg tggttcggcg aaggcgtgaa gcgcagccgc tcggtgccct
     2521 tgggcacagt cggatagttg atcggctgga catagacgcc gtgctcttcc agcagcatgt
     2581 ccgagatcat cttcgtgtgc acgggatcgc ccacgaacac cggcacgata tggctgaccg
     2641 actccatcac cggaatgccg gccgcggcga accgcgcctt cagcgttgcg gcgcgttcct
     2701 gatgcgcctc cctcagctcc ggatgagcct tcaggtgccg cacggacgcc agcgcccccg
     2761 cggtcagagc gggcggcagg ctggtggtga agatgaagcc cgaggcccag ctgcgcaccg
     2821 cgtccacgat caccgcgtcg gcggcgatat agccgcccat cacgccgatg gccttgccca
     2881 gcgtgcattc gacgatgtcg atcccgtcca gcagcccgtc gcgctcggcc acgcccgcgc
     2941 cggtcgcgcc gtacaggccg accgcatgga cctcgtcgag ataggtcatg gcgccgtact
     3001 tcttcgccag ggcgatggtc cccgccaggt cggcgatgtc gccgtccatc gaatagacgc
     3061 tctcgaaggc gatcagcttg ggcgcatcgg ccggcgccgc ggccagcagg tcctccaagt
     3121 gcgccaggtc gttgtggcgg aacaccctgc gctgctcgcg cggaccggtg cggatgccgg
     3181 aaatcatcga gttatggttc agctcgtcgg agaagacgat cagccccggc a
//
LOCUS       DASVVN010000259         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_404287,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000259 DASVVN010000000
VERSION     DASVVN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404287"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1414)
                     /locus_tag="VF686_04095"
     CDS             complement(176..1414)
                     /locus_tag="VF686_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661024.1"
                     /translation="MSDAATPPAEANLSRKAWIMILAGALIVTLSMGVRQAFGLFLRP
                     IGLDLEIDRQTFGLVIAAQNLLFGLIQPFVGAWADKYGAGRVAVGGALVYGVGLLIAA
                     SAADALGLMLGFGVMTGLAMSGVTFVVVLGAVGRIVPPGKRTLAFGIVTAGGSLGQFL
                     VVPAAQGLLDTLDWRITLFVMAALVALIIPLALGVAGKPPASDARDGLPLGEALAEAG
                     RHPSFWLLNLGFFVCGFHIAFVGTHLPAYIRDEGLSAAVGAGALALIGLFNIAGSWLW
                     GAWRSKHSKKGLLALLYALRGVAIALFLIVPLSSASVLVFAAAFGFLWLGTVPLTTGL
                     VGQIYGVRHLSALGGIVFLSHQVGAFLGAWTAGLAFDATGSYAIIWMISIGLAAMAAL
                     ANLPIKEAPLVRPAALQPAE"
     gene            complement(1407..>1823)
                     /locus_tag="VF686_04100"
     CDS             complement(1407..>1823)
                     /locus_tag="VF686_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX8661025.1"
                     /translation="AMNDSTPCICGRLRRTSRALTRLYDEALAPVGITVTQFAVMRTL
                     GRLDRPGLVELAEATAHEKSALWRTLQPLVKKGWVAAEAEKGARGQKLSLTAAGREKL
                     SDALPHWRTAQARVADTLGPREAALIALLAEVEAHV"
BASE COUNT          312 a          617 c          681 g          213 t
ORIGIN      
        1 gcctgtcggc ccagaagggg ctggccgagt tctcggtcat gccgcgcgac gaatagacgt
       61 caggtgcaga aggcgatggc ggcgtcgacc cagaccgcca gcccgttgcg gctccacagc
      121 cagacgccca gcccgagcac ggcggccagt gcgacgccga tgaggatgcg cgtcctcatt
      181 cggcgggctg cagggcggcc gggcggacca gcggcgcctc cttgatcggc agattggcca
      241 gggcggccat ggcggccaga ccgatcgaga tcatccagat gatggcgtag gagccggtgg
      301 cgtcgaaggc cagaccggcc gtccaggcgc ccagaaacgc cccgacctga tggctcagga
      361 acacgatccc gcccagggcc gacaggtggc gaacgccgta gatctggcca accagtccgg
      421 tggtcagcgg cacggtgccc agccacagga agccgaaggc ggcggcgaag accagtacgc
      481 tggccgacga caggggcacg atcaggaaca gggcgatcgc gacgccgcgc agggcgtaga
      541 gcagggccag cagccccttc ttggaatgtt tcgatcgcca tgcgccccac agccacgagc
      601 cggcgatgtt gaacaggccg atcagggcca gggcgccggc cccgacggcg gccgacaggc
      661 cctcgtcgcg gatataggcg ggcaggtggg tgccgacgaa ggcgatgtgg aagccgcaga
      721 cgaagaagcc gaggttcagc agccagaagc tggggtggcg gccggcctcg gccagcgcct
      781 ccccgagagg caggccgtcg cgggcgtcgc tcgcgggcgg cttgccggcg acgccgagcg
      841 ccagcggaat gatcagggcg acgagagcgg ccatgacgaa cagggtgatc cgccagtcca
      901 gcgtgtccag cagtccctgg gccgccggga cgaccagaaa ctggcccagc gatccgccgg
      961 cggtgacgat gccgaaggcg agggtgcgct tgccgggcgg cacgatgcgt cccaccgctc
     1021 cgagcacgac gacgaaggtg acgcccgaca tggcgaggcc cgtcatgacg ccgaagccca
     1081 gcatcaggcc cagcgcgtcg gcggcgctgg ccgcgatcag caggcccacg ccatagacca
     1141 gcgccccgcc gacggcgacg cgaccggctc cgtacttatc cgcccacgcc ccgacgaagg
     1201 gctggatcaa gccgaacagc aggttctggg cggcgatgac gaggccgaag gtctggcggt
     1261 cgatctcgag gtcgaggccg atgggccgca ggaagaggcc gaacgcctgc cgcaccccca
     1321 tcgacagggt gacgatcagg gcgccggcca ggatcatgat ccaggccttg cgcgacaggt
     1381 tcgcctcggc gggcggcgtg gcggcgtcag acatgggctt ccacctcggc cagcagggcg
     1441 atcagggcgg cctcgcgcgg gccgagggta tcggcgaccc gggcctgggc cgtgcgccag
     1501 tggggcaggg cgtcggacag tttttcgcga ccggcggcgg tcagcgacag cttctgcccg
     1561 cgcgcgccct tttcagcctc ggcggcgacc cagccctttt tcaccagcgg ctgcagggtg
     1621 cgccacaggg cgctcttctc gtgggccgtg gcctcggcca gctccaccag accgggccgg
     1681 tccagacgtc cgagtgtgcg catgaccgcg aactgggtga ccgtgatgcc gaccggcgcc
     1741 agcgcctcgt cgtacagccg ggtcagggcg cgggaggtac ggcgcaggcg gccgcagatg
     1801 cagggcgtcg agtcgttcat tgc
//
LOCUS       DASVVN010000260         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_87792, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000260 DASVVN010000000
VERSION     DASVVN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87792"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VF686_04105"
     CDS             <1..470
                     /locus_tag="VF686_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX8661026.1"
                     /translation="RGARDEAYYALEKDACERLRLTLIDAPLDSRDPPQKERVHRARE
                     LFRTIEYPVLIHCKSGADRAGMMAVFYRHFHLGEPISVAIDQLSKKYLHHREGLTGVL
                     DYTLEKYLREVEPTGVSFIDWIDSDAYDPKAIRAEFKAGWWGTVLTERLLKRE"
     gene            complement(475..705)
                     /locus_tag="VF686_04110"
     CDS             complement(475..705)
                     /locus_tag="VF686_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661027.1"
                     /translation="MTLFGRTLGPEEITSLVFLLMVLVLWIGAWRSNRDWTNWFRSWE
                     AGRKERRDAERAAEEGGDQNSSPPGAPRGPWG"
     gene            complement(702..923)
                     /locus_tag="VF686_04115"
     CDS             complement(702..923)
                     /locus_tag="VF686_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661028.1"
                     /translation="MTVPPLIILALIAVGVAIVVWFVRRKPDTPGTGPRDADTAWNDP
                     VTPTPTTADPVRRNDTLSDAPPPSRDPLP"
     gene            complement(992..1240)
                     /locus_tag="VF686_04120"
     CDS             complement(992..1240)
                     /locus_tag="VF686_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX8661029.1"
                     /translation="MTTETDAPQLLHLVIGGELRHLDAPVFRDLSQVEFVGAFPNYEE
                     ARKAWKARAQATVDNAHMRYFILHAHRMIDPRGDDHSH"
     gene            complement(1243..>1555)
                     /locus_tag="VF686_04125"
     CDS             complement(1243..>1555)
                     /locus_tag="VF686_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8661030.1"
                     /translation="PEPWPAMRYEKRNALAYRMALVAAGAFDAAINLTPKWDWDVCAG
                     ALIVEEAGGRVSDHHGGAWRFNQPDPRQNSLICSAPALHPLIVRRTAPIPLAPRQTDR
                     T"
BASE COUNT          243 a          471 c          596 g          245 t
ORIGIN      
        1 tgcgcggggc gcgggacgag gcctattatg cgctggagaa ggacgcctgc gagcggctgc
       61 ggctgaccct gatcgacgcg ccgctggact cgcgcgatcc gccgcagaag gagcgggtgc
      121 accgggcgcg cgagctgttc cggacgatcg aatatccggt gctgatccac tgcaagtcgg
      181 gggcggaccg ggcggggatg atggcggtct tctatcgcca cttccatctg ggcgagccga
      241 tcagcgtggc catcgaccag ctgtcgaaga agtacctgca tcaccgcgag gggctgacgg
      301 gggtgctgga ctatacgctg gagaaatacc tgcgcgaggt cgagccgacg ggggtcagtt
      361 tcatcgactg gatcgacagc gacgcctacg accccaaggc catccgcgcc gagttcaagg
      421 ccggctggtg gggcacggtc ctgaccgaga gactgctgaa gcgggaatag gcgctcagcc
      481 ccacgggccg cgcggggcgc cggggggcga gctgttctga tcgccgcctt cttcggccgc
      541 gcgttcggcg tcgcggcgtt ccttgcggcc agcctcccag cttctgaacc agttggtcca
      601 gtcgcgattg ctgcgccagg cgccgatcca caggaccaac accatcagca gaaagaccag
      661 ggaggtgatt tcctcgggcc ccagggtgcg tccgaacagg gtcacggaag cgggtcgcgg
      721 gacggcggcg gagcgtcgga caaggtgtcg ttccggcgaa ctgggtcggc cgtggtcggc
      781 gtcggcgtaa ccggatcgtt ccaggcggtg tccgcgtccc tgggcccggt gcccggcgtg
      841 tccggcttgc gacggacaaa ccagacgacg atggcgacgc cgacggcgat gagcgccagg
      901 atgatcagag gggggacggt catgcgatct ctccgcagtc aggcgtggca gtatacgcct
      961 gaaagcgcgc cggaccgagg gagggttccg cctagtgcga gtggtcgtcg ccgcgcgggt
     1021 cgatcatccg gtgcgcatgc aggatgaaat agcgcatgtg ggcgttgtcg accgtggcct
     1081 gggcgcgggc cttccacgcc ttgcgggctt cctcatagtt ggggaaggcg ccgacgaact
     1141 cgacctggga caggtcgcgg aacaccgggg cgtcgaggtg gcgcagctct ccgccgatga
     1201 cgaggtggag cagctggggg gcgtcggttt cggtggtcat catcaggtcc ggtcggtctg
     1261 gcggggcgcc agcggaatag gcgctgtgcg gcgcacgatc aacgggtgaa gcgcgggggc
     1321 gctgcagatc aggctgttct gccgcggatc gggctggttg aaccgccatg cgccgccgtg
     1381 gtgatcgctg acccgaccgc ccgcctcctc gacgatcagg gcgccggcgc aaacatccca
     1441 gtcccatttc ggggtcaggt tgatcgcggc gtcgaaggct ccggcggcga cgagggccat
     1501 gcggtaggcg agggcgttgc gcttctcgta gcgcatggcg ggccagggct cgggc
//
LOCUS       DASVVN010000261         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_194370,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000261 DASVVN010000000
VERSION     DASVVN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194370"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VF686_04130"
     CDS             complement(<1..983)
                     /locus_tag="VF686_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8661031.1"
                     /translation="MKIAQVTPLYEAVPPKLYGGTERVVAHLTDALVDLGHDVTLFAS
                     ADAETRARLVPVRDQAIRLDPAPLKSDLAAHMSMLAEVLKRADDFDVIHFHTDMIHFP
                     FFSRCADKTVTTLHGRLDLKDLPGVYERWSEFGLVSISDDQRRPLPMANWKATVHHGM
                     PGDLYAFSPKSEGYLAFLGRISPEKRPDRAIEIATKLNKPLKMAAKVDPQDKVYWETV
                     IRPMVETNPLVEFVGEIGDHQKSAFLGGAEALLFPIDWPEPFGLVMIEAMACGTPVIA
                     FRCGSTPEIIEDGETGFLVDTLEQAVAATGRAHLLDREAIRARFELRFSATA"
     gene            complement(1108..2187)
                     /locus_tag="VF686_04135"
     CDS             complement(1108..2187)
                     /locus_tag="VF686_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX8661032.1"
                     /translation="MSELKTDVVMRGDCIEVLRSLPDACVDMVFADPPYNLQLGGDLL
                     RPDNSLVDAVDDDWDKFDSFAAYDAFTREWLAECRRVLKPEGSVWVIGSYHNVFRLGT
                     AIQDLGFWVLNDIIWRKSNPMPNFKGTRFTNAHETLIWAARSREQKRYTFNYDALKAF
                     NEDTQMRSDWTLALCTGEERIKDADGKKAHPTQKPEGLLHRVLLAATRPGDVVLDPFF
                     GTGTTGAAAKRLGRHFIGIERDETYAKVAEKRIKAVIPAAPEDLVVMGSKRAEVKVPF
                     GALVEAGLLAPGDKLYCPKGDREARVRADGSLVAGELSGSIHKMGALFENAPACNGWT
                     YWRFKTDQGLRSIDTLRAEVRAGMQ"
     gene            2323..3582
                     /locus_tag="VF686_04140"
     CDS             2323..3582
                     /locus_tag="VF686_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661033.1"
                     /translation="MILFAFLQRLFAIASVAVLVLAGWLIWEWYDAKHVAETLNLPDP
                     SPAPLWWGLGLLAFSLLGRWPVLLLLGRGEATGPARRAESLTVPGADGTALHVEVEGA
                     PEGPILLFTHGWGLSARIWAEARADLGKRFGLVFWDLPGLGRSGRPPVGYSIEAFAED
                     LHAVVDGLPTERPVILVGHSIGGMTVQTFCARHPELLNYRIAGIVLENTTHTNPLKTM
                     ILSRVFTALQPLIELACRLDTVLSPVIWLMNWQAYLSGSSHIAARLVGFGARPSREQL
                     DRTVRLITRNSPAVQASGNLAMLRWSVTERLAAIDVPAIVFVGGRDLITKDHAGETIV
                     EALPQGRLVRVEDAGHMGPVEKAGLYNEQIADFADFVRLLRSRAPAPSRKSFLSPDAD
                     LAPPPSTRDKDDQPAPEQGAPRRLPWA"
     gene            complement(3642..>4129)
                     /locus_tag="VF686_04145"
     CDS             complement(3642..>4129)
                     /locus_tag="VF686_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8661034.1"
                     /translation="IEARLEGPCPETVHGDWTRLCHERAGSVLTAVPGLAGFVEDLEC
                     PRCVASNATVEWIELGLARFDLTRGMGPVFSAAVHVTRGKPHPDIFLHAAAEMKADPS
                     RTLVIEDSTAGVLAGVAAGMRVIGLCAGGHIREGHAERLTAAGAHDIALSWDEVARLM
                     A"
BASE COUNT          646 a         1398 c         1356 g          729 t
ORIGIN      
        1 gccgtggccg agaagcgcag ctcgaaccgt gcgcggatcg cctcacggtc cagcagatgt
       61 gctcttccgg tcgcggcgac ggcctgttcc agggtgtcga caaggaagcc ggtttcgccg
      121 tcctcgatga tctcgggcgt cgagccgcag cggaaggcga tcaccggcgt gccgcaggcc
      181 atggcctcga tcatcaccag tccgaagggc tcgggccagt cgatggggaa cagcagggcc
      241 tcggctccgc cgaggaaggc ggacttctgg tgatcgccga tctcgccgac gaattcgacc
      301 agcggattgg tctcgaccat cggcctgatg accgtctccc aatagacctt gtcctgggga
      361 tcgaccttgg ccgccatctt cagcggcttg ttgagtttgg tggcgatttc gatggcccgg
      421 tccggtcgtt tctcgggcga gatgcggccg aggaaggcca gatagccctc ggacttcggc
      481 gagaaggcgt acaggtcgcc cggcatccca tggtgcacgg tggccttcca gttcgccatg
      541 ggcaggggcc ggcgctgatc gtccgagata gagaccaggc cgaactcgga ccagcgttcg
      601 tagacgccgg gcagatcctt cagatccagc cgcccgtgca gcgtggtcac cgtcttgtct
      661 gcgcagcgcg agaagaacgg gaagtggatc atgtccgtgt ggaaatggat cacgtcgaag
      721 tcgtcggcgc gcttcagcac ctcggccagc atgctcatgt gggcggccag atcggacttc
      781 agcggggcgg gatccagccg gatcgcctga tcgcgaacgg gaaccagacg cgcgcgggtc
      841 tcggcgtcgg ccgaggcgaa cagggtgacg tcatgaccaa ggtcgacgag ggcgtcggtc
      901 agatgggcga cgacgcgttc ggtgcccccg tagagcttcg gcggcacggc ttcatacaga
      961 ggagtgacct gcgcgatctt catagcgata tcggagcccc tgacggtgaa cccggtatca
     1021 actatctcgc aactgcgaag ttccccaaac gcaagtattt cccatgcaaa ctgcgcaggt
     1081 taccgcgtta catctgtcgg ctaccggtca ttgcatcccg gcgcggacct cggctcgcag
     1141 ggtgtcgatc gacctgagac cctgatccgt cttgaatcgc cagtaggtcc agccgttgca
     1201 ggccggggcg ttctcgaaca gggcccccat cttgtggatc gagccgctga gttcaccggc
     1261 gaccagcgat ccgtcggccc gtacccgcgc ctcgcgatcg cccttggggc agtacagctt
     1321 gtcgcccggg gccagcagac cggcctcgac cagcgccccg aacggcacct tcacctcggc
     1381 ccgtttggaa cccatgacca ccagatcctc gggcgctgcg ggaatgacgg ccttgatccg
     1441 cttctccgcg accttggcgt aggtttcgtc gcgctcgatg ccgatgaagt gacggcccag
     1501 ccgcttcgcc gccgcgccgg tcgtgccggt gccgaagaag ggatccagca ccacatcgcc
     1561 gggccgcgtc gccgccagca gcaccctgtg cagcaggccc tcgggcttct gcgtcggatg
     1621 ggccttcttg ccgtcggcgt ccttgatccg ctcctcgccc gtgcacagcg ccagggtcca
     1681 gtcggaccgc atctgcgtgt cctcgttgaa ggccttcagc gcgtcgtagt tgaaggtgta
     1741 gcgtttctgc tcgcggctgc gcgctgccca gatcagcgtc tcatgggcgt tggtgaagcg
     1801 cgtgcccttg aagttcggca tcgggttgga cttgcgccag atgatatcgt tcagcaccca
     1861 gaagcccagg tcctgaatcg ccgtgccgag ccggaagacg ttgtgatagc tgccgatcac
     1921 ccagaccgag ccttcgggct tcagcacccg gcggcactcg gccagccatt cgcgggtgaa
     1981 ggcgtcatag gcggcgaagc tgtcgaactt gtcccagtcg tcgtcgaccg cgtcgaccag
     2041 actgttgtcg ggccgcagca ggtcgccgcc cagctgcaga ttatacgggg ggtcggcgaa
     2101 gaccatgtcc acgcaggcgt cgggcaggga ccgcaacacc tcgatgcagt cgcccctcat
     2161 gacgacgtcg gtcttcaact cggacattgg tactcggccc cgctcagctt gaagaccgat
     2221 tggtgaatca tcgcggttaa gggcgcgtaa ccgcgctaaa aatcccagga acgatcagcg
     2281 atagacggtg ttggttcggc gaccccttct ccgagcaccg tgatgatcct gttcgctttt
     2341 ctccaacgcc tcttcgccat cgcgtcggtg gccgtcctcg ttttggccgg ctggctgatc
     2401 tgggagtggt acgatgcgaa acacgtcgcg gagacgctga acctccccga cccgtccccc
     2461 gcgccgctgt ggtggggcct cggtctgctg gcgttcagcc ttctcggccg ctggcccgtc
     2521 ctgttgttgc tgggccgggg cgaagctacg ggtccggccc ggcgtgccga gagcctgacc
     2581 gttccggggg ctgacgggac ggcgctgcat gtcgaggtcg aaggcgcgcc cgaaggcccg
     2641 atcctgctgt tcacgcatgg ctggggcttg tccgcccgca tctgggccga ggcgcgggcc
     2701 gatctgggca aacggttcgg gctggtgttc tgggacctgc ccggccttgg ccgttccggc
     2761 cgcccgcccg tcggctattc gatcgaagcc ttcgccgagg acctgcacgc ggtcgtcgac
     2821 ggtctgccga ccgagcggcc ggtcatcctc gtcggtcaca gcatcggcgg catgaccgtc
     2881 cagacctttt gcgcccggca tccggagctg ttgaactacc gtatcgccgg gatcgtgctg
     2941 gagaacacga cccatacgaa cccgctgaag acgatgatcc tgtctcgcgt cttcacggcg
     3001 cttcagccgc tcatcgagct ggcgtgccgg ctggacaccg tcctgtcgcc ggtgatctgg
     3061 ctgatgaact ggcaggcgta tctgagcggc tcaagccaca tcgcggcccg ccttgtcggc
     3121 tttggcgccc gcccgtcgcg cgagcagctg gatcgcaccg tacggctgat caccaggaac
     3181 tctccggccg ttcaggcgag cggcaatctg gccatgctgc gctggtccgt gaccgagcgg
     3241 ctggcggcga tcgacgtgcc cgcgatcgtg ttcgtcggcg gccgcgatct gatcaccaag
     3301 gaccacgccg gcgagaccat cgtcgaggcg ctgccgcagg gccggctggt gcgcgtcgag
     3361 gacgccggcc acatgggccc ggtcgagaag gccggcctct acaacgagca gatcgccgac
     3421 ttcgccgatt tcgtacgcct gttgcgcagc cgcgctcctg cgccgtcccg gaagtcgttc
     3481 ctgtcgccgg acgcggatct cgcaccgccg ccttccacac gcgacaagga cgatcagcct
     3541 gcgcctgaac agggcgcgcc ccgccgtctt ccgtgggcat agcctgtctg gccaaggttt
     3601 gcggccaacc ctgagccgca gccgttgaga cgaagaacgt ctcaggccat cagccgggcg
     3661 acctcgtccc atgacagagc gatgtcatgg gcgcccgccg ccgtcagccg ttcggcgtgg
     3721 ccctcgcgga tatggccgcc ggcgcacagg ccgatgaccc gcatccccgc cgccacgccc
     3781 gccagcaccc cggcggtgct gtcctcgatg acgagggtgc gcgacggatc ggccttcatc
     3841 tccgcggccg cgtgcaggaa gatgtccggg tgcggcttgc cgcgcgtcac gtgcacggcg
     3901 gcgctgaaca ccggacccat cccgcgcgtc agatcgaagc gcgccagccc aagttcgatc
     3961 cattccactg tcgcgttcga ggcgacgcag cgcggacatt caagatcttc cacgaacccg
     4021 gccagcccgg gtacggccgt cagcacgctg ccggcccgct cgtggcacag gcgagtccag
     4081 tcgccgtgaa ccgtttcggg gcaggggccc tccagacgcg cctcgatca
//
LOCUS       DASVVN010000262         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_509136,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000262 DASVVN010000000
VERSION     DASVVN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509136"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VF686_04150"
     CDS             complement(<1..682)
                     /locus_tag="VF686_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661035.1"
                     /translation="MLSRIFGDRRHAWAFWLGCLVVTAGVVLHLPMYWMGRDMGFHLA
                     GMPMDAGMMWGMALIIGGVGLTAFGLLPAHKPGMTDEVIILAPPEDAPLTRAHWLMCG
                     LLAVALIVDIMKPASLGFVVPGMREEYGLTKAAVAVLPFAALSGTVVGSFVWGWLADL
                     YGRRASILLSSVMFVGTSICGAMPDFWWNVGMCFLMGAAAGGMLPVAYALLAEIMPTR
                     HRGWALVLV"
     gene            822..1601
                     /locus_tag="VF686_04155"
     CDS             822..1601
                     /locus_tag="VF686_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8661036.1"
                     /translation="MTASADTWTVAGRTFNSRLIVGTGKYKTYQENADAAEAAGAEIV
                     TVAVRRVNLTDPNQPMLVDYVKPDRFTFLPNTAGCFTAEDAVRTLRLAREAGGWDLVK
                     LEVLSDTAHLYPDMIETLRALDLLIKDGFQVMVYCTDDVVMAKRLEEAGAAAIMPAAA
                     PIGSGLGIQNRVNIRLIVEQAKVPVLVDAGVGTPSDAVLAMELGCDAVLMNTAIAGAT
                     DPILMASAMKHAVIAGRQGYLAGRMPRRMYASASSPLSGLI"
BASE COUNT          313 a          552 c          604 g          230 t
ORIGIN      
        1 cgacgaggac caaggcccag cctcggtgcc gggtgggcat gatctccgcc agcagggcgt
       61 aggccacggg cagcatgccg ccggcggccg cgcccatcag gaaacacatg ccgacgttcc
      121 accagaagtc cggcatcgcc ccgcagatcg atgtgccgac gaacatcacc gacgacagca
      181 ggatcgaggc gcggcgtccg tagaggtccg caagccagcc ccagacgaag gagcccacca
      241 cggtgccgct gagggcggcg aagggcaaaa cggccacggc ggccttggtc agcccgtatt
      301 cctcgcgcat gcccgggacg acgaagccga ggctggcggg cttcatgatg tcgacgatca
      361 gggccacggc gagcaggccg cacatcagcc agtgggccct ggtgaggggc gcgtcttcgg
      421 gcggggcgag gatgatcacc tcgtccgtca tgccgggctt gtgggcgggc agcaggccga
      481 aggcggtcag gccgacgccg ccgatgatca gggccatgcc ccacatcatg ccggcgtcca
      541 tcggcatgcc ggcgagatgg aagcccatgt cgcggcccat ccagtacatc ggcagatgca
      601 ggacgacgcc cgcggtcacg accaggcagc cgagccagaa ggcccaggcg tggcggcggt
      661 ctccaaagat ccgtgacagc atcggcgacg accccctaaa gtcctacaaa tcccgtccta
      721 cagtcgcgct tgcgcctgcg tcgagcggga atcatccttc gaccggacga ttcgcgcgcc
      781 gggtcttcga aaccggcggc ggacgggcta aacccccgcc catgaccgca tccgcagaca
      841 cctggaccgt cgccggccgg accttcaaca gccggctgat cgtcggcacc ggcaagtaca
      901 agacctatca ggagaacgcc gacgcagccg aggcggcggg agccgagatc gtgaccgtgg
      961 cggtccggcg cgtgaacctg accgacccga accagccgat gctggtcgac tatgtgaagc
     1021 cggaccggtt caccttcctg cccaacaccg cgggctgctt caccgccgag gacgccgtgc
     1081 ggacgctgcg gctggcgcgc gaggcgggcg gctgggatct ggtcaagctg gaggtgctgt
     1141 cggacacggc gcacctgtat cccgacatga tcgagacgct gcgggcgctg gacctgctga
     1201 tcaaggacgg cttccaggtg atggtctatt gcaccgacga cgtggtgatg gcgaagcggc
     1261 tggaggaggc gggggccgcg gccatcatgc cggcggcggc gccgatcggc tcggggctgg
     1321 gcatccagaa ccgggtcaac atccggctga tcgtggagca ggccaaggtt ccggtgctgg
     1381 tcgacgccgg ggtagggacg ccgtcggacg cggtgctggc catggaactg ggctgcgacg
     1441 cggtgctgat gaacacggcc atcgccgggg cgaccgaccc gatcctgatg gcctcggcca
     1501 tgaaacacgc ggtgatcgcc gggcggcagg gctatctggc cgggcggatg ccgcggcgga
     1561 tgtacgccag cgccagttcg ccgctgagcg ggctgatctg aaaaacacag tgcaaggcgt
     1621 gcggtgagtg cggtgagtgc atggcgagaa acacagtcta cagcgtctat agcgtctact
     1681 gcgtctactt ccggaaatc
//
LOCUS       DASVVN010000263         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_353164,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000263 DASVVN010000000
VERSION     DASVVN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353164"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VF686_04160"
     CDS             complement(<1..1076)
                     /locus_tag="VF686_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661037.1"
                     /translation="MNVRSTLLSTVAVLGVLIGASAAYAQTPAAQVIPTEARSALSGG
                     MFDAGQYASGMVLEHSVRPAPGFFEQEALFSPPRTPAEGAALQERIRANPRFAPLALA
                     NSDMAMSNGRLFVGNFNGFNAYDVTKGGAPELVMSVVCPGGQGDVSVVGNLLFMSVEA
                     NRARLDCGTAVAEGDVNADRFRGVRIFDISDLNAPRQIAAVQTCRGSHTHTLVPDPAD
                     PRSLYIYNSGTADVRKTEELAICTDGQPDQNPETALFSIDVIKVNLDRPQEAAIVSRP
                     RIFADANGRVAGLWRGGRAGIASQNTAQTNQCHDITVYPEIGLAAGACSGNGILLDIS
                     NPEAPVRLSDIFDPDMAYWHSATF"
     gene            complement(1097..1768)
                     /locus_tag="VF686_04165"
     CDS             complement(1097..1768)
                     /locus_tag="VF686_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX8661038.1"
                     /translation="MTSLPAILFSGLIAVQAPQSPPIFQPGAPGAAARIITPGEAVAL
                     SRSTFIAADVAFMQHMIVHHAQAVEMVGLLETRGASPAVKLLGRRIALSQQSEMDLMR
                     DWLTQRGQPLEMAGMNHAGMDHSAHASHGAGHAMPASETPVMPGMLSPAQMQTLAAAS
                     GPAFDRLFLQGMIQHHQGALDMVDDLMANPGAAEDSLLSDFTASVVADQSTEILRMQS
                     ILSDL"
     gene            complement(1774..2139)
                     /locus_tag="VF686_04170"
     CDS             complement(1774..2139)
                     /locus_tag="VF686_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661039.1"
                     /translation="MRIQTMLAGLAAAALMAFPAMAQDGQYREPLTRIITEAADGVCL
                     EALMGPALLDACNGQIQGMAPALAAMGAIESMTFVKAEDTPEGPVETWAVKFAGGQTL
                     TWVIGQMQNGKFNTVGVSG"
     gene            2350..>2540
                     /locus_tag="VF686_04175"
     CDS             2350..>2540
                     /locus_tag="VF686_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661040.1"
                     /translation="MKRVLLTTAAVMALAFPAMAQTPEPPSPLAAPPTPAPTPAPTQG
                     PAPVQVQEGSAPAAAPAAAA"
BASE COUNT          368 a          792 c          981 g          399 t
ORIGIN      
        1 ttgaaggtgg ccgagtgcca gtaggccatg tcggggtcga agatgtcgga caggcggaca
       61 ggagcctcgg gattggagat gtccagcagg atgccgttgc cgctgcaggc gccggcggcc
      121 aggccgatct ccggatagac ggtgatgtcg tggcactggt tggtctgggc ggtgttctgg
      181 ctggcgatgc cggcgcggcc gccacgccac aggccggcga cgcggccgtt ggcgtcggcg
      241 aagatgcgtg ggcggctgac gatggcggct tcctgcgggc ggtcgaggtt gaccttgatc
      301 acgtcgatcg agaacagggc ggtctcgggg ttctggtccg gctggccgtc ggtgcagatg
      361 gccagttcct cggtcttgcg cacgtcggcg gtgcccgagt tgtagatgta caggctgcgc
      421 ggatcggcgg ggtcgggcac cagggtgtgg gtgtgcgaac cgcggcaggt ctggaccgct
      481 gcgatctggc gcggggcgtt caggtcgctg atgtcgaaga tgcgcacgcc gcggaagcgg
      541 tcggcgttga cgtcgccctc ggccacggcc gtgccgcagt cgaggcgggc gcgattggcc
      601 tcgaccgaca tgaacagcag gttgccgacg acggagacgt cgccctgacc gccggggcag
      661 accaccgaca tgaccagttc gggcgctccg cccttcgtca cgtcataggc gttgaagccg
      721 ttgaaattcc cgacgaacag acggccgttg gacatggcca tgtcgctgtt ggcgagggcc
      781 aggggggcga agcgcgggtt ggcgcggatc cgctcctgaa gcgcggcgcc ctcggccggt
      841 gtgcgcggcg gcgagaacag ggcctcctgc tcgaagaagc cgggggcggg ccggaccgaa
      901 tgttccagca ccatgccgct ggcgtactgg ccggcgtcga acatgccgcc ggacagggcg
      961 ctgcgggctt cggtcgggat gacctgggcg gcgggcgtct gggcgtatgc cgcgctggcg
     1021 ccgatcagga cgccgagaac ggcgacggtg gaaagcaggg tgctgcgaac gttcatggcc
     1081 gctccttggt gcgaggtcag aggtctgaga ggatggattg catgcggaga atttccgtcg
     1141 actgatcggc gacgacggag gcggtgaaat cggacaacag ggagtcttcg gcggcgccgg
     1201 gattggccat cagatcgtcg accatgtcca gcgcgccctg atgatgctgg atcatcccct
     1261 gcaggaacag gcggtcgaag gcggggccgc tggcggcggc gagggtctgc atctgcgcgg
     1321 gggacagcat gccgggcatc acgggcgttt cgctggcggg catggcgtgg ccggccccat
     1381 gactggcgtg ggccgagtgg tccatgccgg cgtgattcat gccggccatc tcgagcggct
     1441 ggccgcgctg ggtcagccag tcccgcatca ggtccatttc ggactgctgg ctgagggcga
     1501 tgcggcggcc cagaagtttg acggcggggc tggccccgcg ggtctccagc aggccgacca
     1561 tctccacggc ctgggcgtgg tgcacgatca tatgctgcat gaaggcgacg tcggcggcga
     1621 tgaaggtcga gcggctcagc gccaccgcct cgccgggcgt gatgatccgc gccgccgcgc
     1681 cgggggcgcc gggctggaag atcggcggcg attgcggcgc ttgcaccgcg atcagtccgg
     1741 agaagaggat ggccggcagg ctggtcaatc cggctagccc gagacgccga cggtattgaa
     1801 cttgccgttc tgcatctggc cgatgaccca ggtcagggtc tggccgccgg cgaatttaac
     1861 ggcccaggtc tcgacgggac cttcgggggt atcctcggcc ttgacgaaag tcatggattc
     1921 gatcgcgccc atggcggcga gcgccggggc catgccctgg atctgtccgt tgcaggcgtc
     1981 gagcagggcc ggccccatga gcgcctcgag gcagacgccg tcggcggcct cggtgatgat
     2041 ccgggtcagc ggctcgcggt actggccgtc ctgggccatg gccgggaagg ccatcagggc
     2101 ggcggcggcg aggccggcca acatggtctg gatacgcatt cgtctcttgt gtcctgtttg
     2161 tggcgggaac catgacgcag gcggcgcacg gtcacaacct gtccccgcat gacaaagaat
     2221 tcacgcaagg ccgcggcggc gcggcggtcg gacggctgga cttggcccgc cccgcaatgc
     2281 tctgttgccg gccgacaagg acgcagcgcc ccggggcggt tcaggcgtcc ggtatcgggg
     2341 catgaaacga tgaagcgcgt actcctgacg acggccgccg tgatggcgct cgccttcccc
     2401 gccatggccc agacgcccga gcccccgtcg ccgctggcgg ccccgccgac gcctgcgccg
     2461 acgcccgcgc cgacgcaggg tcccgctccg gttcaggtgc aggagggctc ggcccccgcc
     2521 gcggctccgg ccgccgccgc
//
LOCUS       DASVVN010000264         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_194502,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000264 DASVVN010000000
VERSION     DASVVN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194502"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VF686_04180"
     CDS             <1..1204
                     /locus_tag="VF686_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX8661041.1"
                     /translation="MLAATDEHIALHRELVRQADRLYGARHFNHYDFLVAVSDDLGGI
                     GLEHHRSSENSVDTRYFTDAQATIGDRDLLGHEYTHSWNGKWRRPADQLTPTLNEPLQ
                     NSLLWVYEGQTQYWGLILTARAGLMTKQQALDVFANTAATYAEDNPGRTWRAMQDTTN
                     DPIIAQRRPQPWGSFQRSEDYYREGAMIWLDADTLIREQTNGRKSLDDFARAFFGVQD
                     GNWDPAPYTFTDVTSTLSGVQANDWAEFLRTRLDAVGVNATTPLGGIQRGGYRLVWRE
                     TPNDYSKALNKDYSRVDFAYSLGLSLNPASRVTAVRWGSPAFEAGLTSGWEVVAVNGR
                     AASADAISEAITASKGNTTPIEMMLKKGDRFRTIAFDYHGGLRYPHLERIPGTPDRLG
                     DILAPRRR"
     gene            1222..>1535
                     /locus_tag="VF686_04185"
     CDS             1222..>1535
                     /locus_tag="VF686_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX8661042.1"
                     /translation="MIRTAVLALLLTSAALPAFAQVPASQAATPLPAPTSLPRPLPPI
                     PAPQDRDYPGVIGIQADLTDLDHKVIRVRQTVPVSAGHLVLQYPKFIPGNHADTGPIQ
                     LVS"
BASE COUNT          264 a          621 c          431 g          219 t
ORIGIN      
        1 catgctggcc gccaccgacg agcacatcgc cctgcaccgc gaactggtcc gtcaggccga
       61 ccgcctgtac ggcgcgcgcc acttcaacca ctacgacttc ctcgtcgccg tcagcgacga
      121 cctcggcggc atcgggctgg agcatcaccg ctcgtccgag aacagcgtcg acacccgcta
      181 tttcactgac gcccaggcaa ccatcggcga tcgtgacctt ctcggccacg aatacaccca
      241 ctcctggaac ggcaaatggc gccggcccgc cgaccagctg acccccacgc tgaacgagcc
      301 gctgcagaac tccctgctct gggtctatga gggccagacc cagtactggg gcctgatcct
      361 gaccgcccgc gccggcctga tgaccaaaca gcaggctctg gacgtcttcg ccaacaccgc
      421 cgcgacctat gccgaggaca accccggccg gacatggcgc gcgatgcagg acaccaccaa
      481 cgatccgatc atcgcccagc gccggcccca gccatggggc agcttccagc gcagcgagga
      541 ctactatcgc gaaggggcaa tgatctggct cgacgccgac accctgatcc gcgagcagac
      601 caatggccgc aagtcgctgg acgacttcgc ccgcgccttc ttcggcgttc aggacgggaa
      661 ttgggatccg gcgccctata ccttcaccga cgtgacctcg acgctgagcg gcgttcaggc
      721 caacgactgg gccgagttcc tgcgcacccg cctcgacgcc gtcggcgtca acgccacgac
      781 cccgctgggg ggcatccagc gcggcggcta ccgcctcgtc tggcgcgaga cgccgaacga
      841 ctattcgaag gcgctgaaca aggactacag ccgcgtcgac ttcgcctatt cactgggcct
      901 cagcctgaac ccggccagcc gcgtcaccgc cgtccgctgg ggctcgcccg ccttcgaggc
      961 cggcctgacc tccggttggg aggtcgtcgc cgtcaacggc cgcgccgcca gcgccgacgc
     1021 catctccgaa gccatcaccg cctcgaaggg caacacgaca cccatcgaga tgatgctgaa
     1081 gaagggcgac cgcttccgca ccatcgcctt cgactatcac ggcggcctcc gctatccgca
     1141 cctcgagcgg atccccggaa cgcccgaccg tctcggcgac atcctcgccc cccgccgccg
     1201 ttgatcgtga gggacaatca gatgatccgc accgccgtcc tcgcccttct gctgacctcg
     1261 gccgccctgc ccgctttcgc gcaggtcccg gcctcgcagg ccgccacccc cctgcccgcc
     1321 ccgaccagcc tgccgcgccc gttgccgccc atcccggcgc cgcaggaccg cgactatccc
     1381 ggcgtcatcg gcatccaggc cgacctgacc gatctcgacc acaaggtcat ccgcgtgcgc
     1441 cagaccgtcc cggtctcggc gggccatctg gtcctgcagt atccgaaatt catccccggc
     1501 aaccacgccg acaccggccc gatccagctg gtctc
//
LOCUS       DASVVN010000265         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_482309,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000265 DASVVN010000000
VERSION     DASVVN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482309"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VF686_04190"
     CDS             <1..509
                     /locus_tag="VF686_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506791.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX8661043.1"
                     /translation="IGARVTVVPASTAAEDILARKPDGVLLSNGPGDPAATGEYAVPE
                     IRKLVDSGTPVFGICLGHQMLALALGAKTIKMDQGHHGANHPVKDLTTGKVEIVSMNH
                     GFTVDRDSLPDAVTETHVSLFDGTNCGIALTDRPVFSVQHHPEASPGPTDSLYLFQRF
                     ADLMRPRP"
     gene            712..1260
                     /locus_tag="VF686_04195"
     CDS             712..1260
                     /locus_tag="VF686_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX8661044.1"
                     /translation="MSPGLRLYEPNPKMFAILDAARPANAVARNIALSDRPGIASLKV
                     PRSARGYSNQHASLETNWAVAVEFGVVEVATARLDDLDLEPIGFIKIDVEGHEQAVVA
                     GAVGLIARDRPTLLIELEERHSEQSIEASIAAVKALGYDAFVFKDGALRPIAAFDPDA
                     DHRAAVETPRYIFNFIFKPRLP"
     gene            1335..2582
                     /locus_tag="VF686_04200"
     CDS             1335..2582
                     /locus_tag="VF686_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX8661045.1"
                     /translation="MTAQPVETAHDAPLATPATPATSAGGLTRSGAVRIDSLDMLRGL
                     VIVLMVLDHVRDFFHAAAFTADPMALDSGNPALFLTRWATHLCAPTFVFLAGLSVWLQ
                     KANGKAPGDLSRFLVTRGVWLIVLEFTLIGLGFNFGWWLFAQVIWAIGFGMIVLAGLH
                     RLPRLVVLAVGVAIIVAHPALNLVSPSTFGPLEPLWRMLAVRGALPTEAGLLLVAYPA
                     IQWTGILLLGYGMAGFLISAGGLRRDRVMLAAIGFLALFAVGRGLNLPGFDPNPWAGQ
                     EGPLWTAFSIISVNKYPPSPAYVLLMIGLAFLILLAFERLRGPVMKPLLTFGRTPLFT
                     YLLHVWIAHALAVAVGVAVGVPPAVFVNMITDPSRVIEAGWGFNLGGVYLAWLAVLVI
                     LYPLSRWFEGVKRRRRDWWLGYL"
     gene            complement(2579..2845)
                     /locus_tag="VF686_04205"
     CDS             complement(2579..2845)
                     /locus_tag="VF686_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661046.1"
                     /translation="MFLSIARPAVEPRGPDAVAVPGSPAITDLSPRALHARLLQNAAL
                     RRMRGAERLRSNRLEDADYWLHAAPVAVRKASALREEKSFAPLP"
     gene            3071..>3291
                     /locus_tag="VF686_04210"
     CDS             3071..>3291
                     /locus_tag="VF686_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX8661047.1"
                     /translation="MRFDDRFLEELKARLRPSDIIGRTVKLKRQGREYVGLSPFTKEK
                     SPSFFVNDDKGFFHDFSSGKHGDVISFLQ"
BASE COUNT          500 a         1087 c         1081 g          623 t
ORIGIN      
        1 ccatcggcgc tcgcgtcacc gtggtgccgg cctcgacggc ggccgaggac atcctggccc
       61 ggaagccgga cggcgtcctg ctctccaacg gcccgggcga tcctgccgcc accggcgaat
      121 acgccgtgcc ggagatccgc aagctggtcg acagcggcac gccggtgttc ggcatctgcc
      181 tgggccacca gatgctggcg ctggcgctcg gggccaaaac catcaagatg gatcaggggc
      241 accacggcgc gaaccatccg gtgaaggacc tgaccaccgg caaggtcgag atcgtatcca
      301 tgaaccatgg cttcaccgtc gaccgcgaca gcctgcccga cgcggtcacc gagacccacg
      361 tcagcctgtt cgacggcacc aactgcggca tcgccctgac ggaccgcccg gtgttcagcg
      421 tccagcatca ccccgaggcc tcgcccgggc ccactgacag cctttatctg ttccagcggt
      481 tcgccgatct gatgcgcccg cgtccgtgaa ctggacccca ccgtcaacgc ttagggagaa
      541 actgaagcgt gcggtgttca gtccacggca ggaactgaag cggattgttg cgcgggagct
      601 gcggcgcggg gagcccgagc tgaggctgct gcccgaactg gtcgatccgg aacgcgcgtc
      661 tgtcgatgtg ggcgccaatc gcggcgtctg gacccaccag ctggcgacgc tgtgtccccg
      721 ggtctacgcc tctatgagcc caatcccaag atgttcgcca ttctggatgc ggcgcgtccc
      781 gcgaatgctg tggcgcggaa catcgccctg tcagatcggc cgggcatagc cagcctgaag
      841 gtgccgcggt cggcgcgggg ctattccaac cagcatgcct cgctggagac caactgggcc
      901 gtcgccgtgg agttcggcgt ggtcgaggtg gccacggcac gtctggatga tctggatctc
      961 gagcccatcg gcttcatcaa gatcgatgtc gagggccatg agcaggccgt agtcgccggc
     1021 gcggtcggcc tgatcgcccg cgaccggccc acgctgctga ttgagctgga ggaacggcac
     1081 tcggaacaat cgatcgaggc ttcgatcgcc gccgttaagg cgttgggcta cgacgcgttt
     1141 gtgttcaagg acggcgcttt gcggccgatc gctgccttcg atccggacgc cgatcatcgg
     1201 gccgccgtcg aaaccccccg ctatattttc aacttcatct tcaagcctcg ccttccctga
     1261 acgcgtccgc cggctctggc gtgtgcggcg agagcatgca caatgggcgc cccggacctc
     1321 aggggaggac agccgtgacc gcacaacccg tcgagacggc tcatgacgcg ccgctcgcca
     1381 cgcccgccac gcccgccacg agcgcaggcg gcctgacgcg ctccggcgcg gtgcggatcg
     1441 actcgctcga catgctgcgc ggcctggtca tcgtactgat ggtgctggac catgtgcgcg
     1501 acttcttcca cgccgcggcc ttcaccgccg atccaatggc gctggattca ggcaatcccg
     1561 cgctgttcct gacccggtgg gcaacccacc tgtgcgcgcc gaccttcgtc tttctcgccg
     1621 gcctttccgt ctggctgcaa aaggcgaacg gaaaggcgcc gggcgacctg tcgcgctttc
     1681 tggtgacgcg cggggtctgg ctgatcgtgc tggagttcac cctgatcgga ctcggcttca
     1741 atttcggatg gtggctgttc gcccaagtca tctgggccat cggcttcggc atgatcgtgc
     1801 tggccggatt gcaccgtctg ccgcgcctcg tggtgctggc cgtaggcgtc gccatcatcg
     1861 tcgctcaccc ggcgttgaac cttgtctccc cctcgacctt cgggccgctt gagccgctct
     1921 ggcggatgct cgcggtgcgc ggggcgctgc cgacggaggc cggactgctg ctcgtcgcct
     1981 atccggcgat ccagtggacc ggaatcctgc ttctcggcta cgggatggcg ggcttcctga
     2041 tcagcgccgg ggggctgagg cgggacaggg tgatgctggc ggcgatcggg tttctggcgc
     2101 tattcgcggt ggggcgaggg ctcaacctgc cgggcttcga tccaaatccc tgggcggggc
     2161 aggagggtcc gttgtggacg gcgttcagca tcatcagcgt caacaagtat ccgccgtcgc
     2221 cggcctatgt gctgctgatg atcggtctgg ccttcctcat cctgctggcg tttgagcgcc
     2281 tgcgcggacc ggtgatgaag ccgctgctga ccttcggccg cacgccgctg ttcacctatc
     2341 tgctgcacgt ctggatcgcg cacgccctgg cagtggcggt cggggtcgcg gttggcgtcc
     2401 cgcctgccgt tttcgtcaac atgatcaccg acccttcgcg cgtcatcgaa gccggatggg
     2461 gcttcaatct gggcggcgtt tacctcgcct ggctggccgt gctggtcatt ctctatccgc
     2521 tgtcgcgctg gttcgagggc gtgaaacgcc gtcgccgcga ctggtggctc ggctacctct
     2581 agggcagcgg cgcgaagctt ttctcttccc gcagggcgct ggccttgcgc accgcgaccg
     2641 gcgcggcgtg cagccagtag tcggcgtctt ccagccggtt cgagcgcagg cgttcggccc
     2701 cgcgcatgcg ccgcagggcc gcgttctgca gcaggcgggc gtgcagggcg cggggcgaaa
     2761 ggtcggtgat tgcgggcgag ccggggacgg cgacggcgtc gggccccctt ggttcgaccg
     2821 caggacgggc gatactcaag aacatggatg gtctctctca ctctcggcag gaaaacggtc
     2881 ttcgccgccg cccgcccgaa cctgtccttc ttcacggacg atcgtatggt taatccacag
     2941 gcctgtggat tgttccgatt tgcgcagaaa aatacgcgta cattcaggca gttatggtca
     3001 cgccttggtc tttttggtta acgaaaggta aagggcccca aagcggtgac tcgcgcgcta
     3061 taaggcgagg gtgcgcttcg acgaccgctt ccttgaagaa ctgaaggccc gccttcggcc
     3121 gtccgacatc atcggacgga cggtgaagct caagcgtcag ggacgcgaat atgtcggcct
     3181 gtcgcccttt accaaggaga agtcgccgtc cttcttcgtc aatgacgaca agggtttctt
     3241 ccacgacttc tcgtcgggca aacacggcga cgtcatttcg ttcctgcagg a
//
LOCUS       DASVVN010000266         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_324417,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000266 DASVVN010000000
VERSION     DASVVN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324417"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1564
                     /locus_tag="VF686_04215"
     CDS             <1..>1564
                     /locus_tag="VF686_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661048.1"
                     /translation="GSVLGFMDGAADAGDIYKVNLVAGQYYEFQVSGGWDAEAGVEGD
                     LRLSLYGPNGLGIFSNNDISSADQNSAMGFVATVTGTYFLQVRNDATPAAGSSPTSGY
                     ELNVITKDISALSPLDAINWGGDDNVVPGAADGEIKVYFAGVGETFWGVTSLGWNDYE
                     KQQAMLAFDQYEKVLPVNFVITDSAADADFKLVTKNTQPGETPGILGRMGPPGTGENA
                     GVAIFWRDGFGWNEEGGGGLQQGGYAGFNTLVHEFGHGMGLSHPHDNGGGSAVMPGVF
                     GPFDHVGASMLNQGIYTVMTYNPGWRQHPDGLFTVPVFTTAQGTFQETQYGYTGGLSA
                     LDIAVLQNKYGANESYNTGDNTYVLPSANQPGTFFTTIWDAGGTDTIQHDGAGPAQID
                     LTAATLDYSATGGGGMSFARTIYGGFTIANGVVIENATGGSGDDVLIGNSADNVLNGR
                     GGVDTVSYERAANGVTVSLAVLGAQATGGAGADTLIDVENLSGSKFDDTLTGNGGNNI
                     LAGGLGNDVLDGA"
BASE COUNT          278 a          535 c          533 g          218 t
ORIGIN      
        1 ggctccgtgc tcggcttcat ggacggcgcc gcggacgcgg gcgacatcta caaggtcaac
       61 ctggtcgccg gtcagtatta cgagttccag gtgtccggcg gctgggacgc cgaagcgggc
      121 gtcgaaggcg acctgcgcct gtcgctgtac ggcccgaacg gcttgggcat tttcagcaac
      181 aacgacatct cctcggccga ccagaactcc gcgatgggat tcgtggcgac ggtcaccggc
      241 acctacttcc tgcaggtgcg caacgacgcg acccccgccg ccggctcctc cccgacgagc
      301 ggctatgagc tgaacgtcat caccaaggac atcagcgcgc tcagccctct ggacgccata
      361 aactggggcg gggacgacaa cgtcgttccg ggcgcggctg acggcgagat caaggtctac
      421 ttcgccggcg tcggcgagac cttctggggc gtcacctcgc tcggctggaa cgactacgag
      481 aagcagcagg cgatgctggc cttcgaccag tacgagaagg tgctgccggt caacttcgtg
      541 atcaccgaca gcgcggcgga cgccgacttc aagctggtga ccaagaacac gcagccgggc
      601 gagacgccgg gcatcctcgg ccgcatgggc cctccgggca ccggcgagaa cgccggcgtc
      661 gccatcttct ggcgcgacgg tttcggctgg aacgaggaag gcggcggcgg cctgcagcag
      721 ggcggctacg ccggcttcaa cacgctggtg cacgaattcg gccacggcat gggcctgtcg
      781 cacccgcacg acaacggcgg cggctcggcg gtgatgccgg gcgtgttcgg cccgttcgac
      841 cacgtcggcg cctcgatgct gaaccagggc atctacacgg tgatgaccta caacccgggc
      901 tggcgtcagc acccggacgg tctgttcacg gtgccggtgt tcaccaccgc gcaggggacc
      961 ttccaggaaa cccagtacgg ctacaccggc ggcttgtcgg cgctcgacat cgcggtgctg
     1021 cagaacaagt acggcgccaa cgagagctac aacacgggcg acaacaccta cgtgctgccg
     1081 tcggcgaacc agccgggcac cttcttcacc accatctggg acgccggcgg gacggacacc
     1141 atccagcatg acggcgccgg cccggcgcag atcgacctga ccgcggcgac gctcgactac
     1201 tcggcgacgg gcggcggcgg catgtcgttc gcgcgcacca tctacggcgg cttcaccatc
     1261 gccaacggcg tggtgatcga gaacgccacc ggcggctcgg gcgacgacgt gctgatcggc
     1321 aacagcgcgg acaacgtgct gaacggccgc ggcggcgtgg acaccgtgtc ctacgagcgg
     1381 gcggcgaacg gcgtgacggt cagcctggcg gtactcggcg cgcaggccac cggcggcgcg
     1441 ggcgccgaca cgctgatcga cgttgagaac ctatcgggtt cgaagttcga cgacaccctc
     1501 accggcaacg gcgggaacaa cattctggcc ggcggcctgg gcaacgacgt cctcgacggc
     1561 gccg
//
LOCUS       DASVVN010000267         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_10083, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000267 DASVVN010000000
VERSION     DASVVN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10083"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF686_04220"
     CDS             complement(<1..767)
                     /locus_tag="VF686_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8661049.1"
                     /translation="MSLLARLAPHLPYVRRYARALTGDQTTGDNYVRVALEALAAGER
                     QLPADMTPRVALYHVFHAIWTTTGAQLEDKSGLESRNDDASRRLMRIAPRSRQAFLLT
                     ALEGFTPSEAAQILDADAFDVERLISEAQSDIDAELATDVLIIEDEAIISADIESLVK
                     ELGHRVTGTATTHDEAVDAVARHRPGLVLADIQLADGSSGIDAVKDILKRIDVPVIFI
                     TAFPERLLTGERPEPTFLITKPFDSDTLHISISQALA"
     gene            959..1159
                     /locus_tag="VF686_04225"
     CDS             959..1159
                     /locus_tag="VF686_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEX8661050.1"
                     /translation="MTDNSNPPGKGPPKGGASLEEARLRQQAIGVKLRHMFDEVVNEQ
                     VPDEFLDILRRADERSSGGSGS"
     gene            1156..1767
                     /locus_tag="VF686_04230"
     CDS             1156..1767
                     /locus_tag="VF686_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8661051.1"
                     /translation="MSGATNPPPKPPSADDDAFKRELVALIPHLRAFARTLTGDPTAA
                     DDLAQDAMMKAWDARASYQMGTNMKAWTFMILRNQFYSEKRRSWRQSQLDQEAAERTL
                     VAVDDPESPVALDELRLALATLPEEQREALILVGAGGFAYEEAAEICQCAVGTVKSRV
                     SRARKALQATLERGGYARDGRAAGDAMRSILAAADRLSSVRQD"
     gene            1790..>2276
                     /locus_tag="VF686_04235"
     CDS             1790..>2276
                     /locus_tag="VF686_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8661052.1"
                     /translation="MLTRGASRSLGPPQFHGIRFRLGWTMAVALLPILVLGAFQTQSA
                     FRAQDIERRSDLQLAAERTAANAKSHLDSTGVLLQALRPEALEVFCEPRLTDLVERLE
                     NLDGLARLTATGAAVCASRGLSQTPPAWLAEAPVSPWFLRLKAGDSMVLARAPTVAGR
                     SP"
BASE COUNT          369 a          737 c          795 g          375 t
ORIGIN      
        1 atggccagag cctgggagat cgagatgtgc agcgtgtcgg aatcgaaggg cttggtgatc
       61 aggaaggtgg gctcgggccg ctccccggta agcagccgct ccgggaaggc ggtgatgaag
      121 atcaccggca cgtcgatccg cttgaggatg tctttgaccg cgtcgatgcc cgacgaaccg
      181 tcggccagct ggatgtcggc caggaccaga cccggccggt ggcgcgcgac ggcgtcgacg
      241 gcttcgtcat gggtggtggc ggtgccggtg acgcgatggc cgagctcctt caccaggctc
      301 tcgatgtcgg ccgagatgat ggcctcgtcc tcgatgatca ggacatcggt ggccagttcg
      361 gcgtcgatgt ccgactgggc ttccgagatc agccgttcga cgtcgaaggc gtcggcgtcc
      421 aggatctggg cggcttcgga cggggtgaac ccttccagcg cggtcagcag gaaggcctga
      481 cgcgaacgcg gtgcgatacg catcagacgg cgcgaggcgt cgtcgttgcg gctttccagc
      541 ccgctcttgt cttccagctg ggcgccggtg gtggtccaga tggcatggaa cacgtgatag
      601 agggcgaccc gcggggtcat gtcggcggga agttgccgtt cgccggcggc gagcgcctcg
      661 agagcgacgc ggacatagtt gtccccggtg gtctgatcac cggtcagcgc ccgcgcgtaa
      721 cggcggacat agggcaggtg cggcgcaagg cgggccagca ggctcatata ataaatcccc
      781 gacggaaggc ggttccggac atggaacctg agcagccccg acaacgtatc aaatacgcca
      841 cggttcccgg tccgggaaca agcttgagca ttatttgtcc ggatcgtagg aaccggccct
      901 gcagagtggc gtttggccct cgcacaagtc tttcgtcagg gtggcggccg ttttttcaat
      961 gacagataat tccaatcctc cgggcaaggg tccgcccaag ggcggcgcga gtctggagga
     1021 agctcgactt cgccagcagg cgatcggcgt caagctgcgc catatgttcg atgaggtcgt
     1081 gaacgagcag gttccggacg agttcctgga catcctgcgg cgcgccgacg agcggtcgtc
     1141 gggcgggagc ggctcatgag cggtgcgaca aacccgccgc ccaagcctcc ttcagccgat
     1201 gacgatgctt tcaagcggga gctggtcgcg ctgattccgc atctgcgcgc cttcgcccgc
     1261 acgctgaccg gcgacccgac ggccgccgac gacctggcgc aggacgccat gatgaaggcg
     1321 tgggacgccc gcgccagcta tcaaatgggc accaatatga aggcctggac cttcatgatc
     1381 ctgcgcaacc agttctactc cgagaagcgc cgctcgtggc gccagtcgca gctggatcag
     1441 gaagcggccg aacgcaccct ggtcgcggtc gacgacccgg aatcgcctgt ggcgctggac
     1501 gaactgcgtc tggccctggc gaccctgccc gaagaacagc gcgaggcgct gatcctcgtc
     1561 ggcgccgggg gctttgccta tgaagaggcg gccgagatct gccaatgcgc ggtggggacg
     1621 gtgaaaagcc gcgtctcccg cgcccgcaag gccctgcagg ccaccctcga acgcggcggc
     1681 tacgcccgtg atggacgcgc cgccggcgac gccatgcgct ccatcctcgc cgccgcggat
     1741 cgcctgagca gcgtccggca ggactgaccg tgtgagccgg atcggcagag tgctgaccag
     1801 aggcgcgagc cgcagtctgg gcccgccgca attccatggc atccgcttcc gtctgggctg
     1861 gacgatggcg gtcgccctgt tgcccattct ggttctgggg gccttccaga cccagtccgc
     1921 cttccgggcc caggatatcg agcggcggtc tgacctgcag ttggccgccg aacgcaccgc
     1981 ggccaacgcc aaatcccatc tggacagcac cggcgtgctg cttcaggcgc ttcggcccga
     2041 ggcgctggag gtcttctgcg agccgcgtct caccgatctg gtcgaacggc tcgagaatct
     2101 ggacgggctc gcgcggctga ccgccaccgg cgcggcggtc tgcgcctcgc gcggtctcag
     2161 ccagaccccg cctgcttggc tggccgaggc gccggtcagt ccgtggtttc tgcggcttaa
     2221 ggcgggggac agcatggtcc tggcccgggc gcccaccgtc gccggccgct cgcccg
//
LOCUS       DASVVN010000268         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_509369,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000268 DASVVN010000000
VERSION     DASVVN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509369"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF686_04240"
     CDS             <1..246
                     /locus_tag="VF686_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8661053.1"
                     /translation="GNGGSHLRSWFKAATGMTVHRYLVVERVRRARDLLVEGRLSSDE
                     IAWATGFSHQSHMACWIRRELGCSPRDIRSGRRSGDR"
     gene            complement(483..1010)
                     /locus_tag="VF686_04245"
     CDS             complement(483..1010)
                     /locus_tag="VF686_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8661054.1"
                     /translation="MAWAVVGRDGADALTLARLAAEAGVAKPVVYSHFPHRNDLLLAL
                     YQEFDEDQSLRLEAALREAEVTLPARAEALSRCYVDCFVSQTRRIPGVSAALDAAPDL
                     SRHKAYCERRYGERFRAALAPFASHGTVSRATLRGILGAADGLSRGAAAGDISEDEAR
                     RELCRLIRSAIFSST"
     gene            1150..1752
                     /locus_tag="VF686_04250"
     CDS             1150..1752
                     /locus_tag="VF686_04250"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8661055.1"
                     /translation="MHVLIVTARPGEASLTQALGRAVADGVEAAGATAEIADLASEGF
                     DPRFTARDIEVHLATAVPDADVTAEQRRLDRADALVLVYPIYWWSFPGLLKGWVDRVF
                     CRGWAYEVNEADAVIEKRLTNLTVHLVAVGGAGLRTYARHGYFGAMKLQIDHGVFDYC
                     GATVATSELMADPVPEAALEHARAIGRRAALVRGALHRAA"
BASE COUNT          320 a          707 c          742 g          330 t
ORIGIN      
        1 ggaaacggcg gctcacatct tcgcagctgg ttcaaagccg cgaccggcat gacggtgcac
       61 cgctatctcg tcgtggagcg ggtccgacgc gcgcgagacc tgttggtcga gggacgcctg
      121 agctccgacg agatcgcctg ggcgaccggc ttctcgcatc aatcccatat ggcctgctgg
      181 atccggcggg aactgggctg ctctccgcgc gacatccgct ccgggcgccg tagcggagac
      241 cggtgaggcg cgcggagcgg acgcgggttc gatcgaacgc cggggcggcc gccccgttgg
      301 gccttctgcg acgcgccgac cggctttcgg cgccggcgcg ggccgctcgg gtcccgggcg
      361 atcgacggga cgaccttgcc gcggcgacgt gcgggcgaac ggcgtcgtga atatggggtg
      421 tgtccgacgc cccgcccgcg cccggtgtcg aacaccggcc gaagaccgac ccgcaaacgc
      481 cttcaggtgg aggaaaagat cgcggaacgg atcagccggc aaagctcccg gcgggcttcg
      541 tcttcggaaa tgtcgcccgc cgccgcgccc cgcgagagac cgtcggcggc ccctagtatt
      601 ccgcgaaggg tcgcccggga caccgtcccg tgggacgcga acggggcaag cgcggcccgg
      661 aatctctctc cgtagcggcg ttcacagtac gccttgtgtc tggacaggtc gggagccgcg
      721 tccagcgccg cgctgacgcc cggtatccgc cgggtctgcg agacaaaaca gtccacgtag
      781 caacggctga gcgcctccgc gcgcgccggg agcgtcactt ccgcctcccg cagcgctgct
      841 tccagacgca ggctctggtc ctcatcgaac tcctggtaca gcgcaagcag aagatcgttc
      901 ctgtggggga agtgactgta gacgacaggc ttggccacgc ccgcctcggc ggcgagccgg
      961 gcgagggtca acgcgtcggc gccgtccctg cccacgacgg cccaggccac gtccagcaac
     1021 tgacggcggc gatccccccg cgccattcgc cgtcggggag cctcggatgc ggctgttgac
     1081 atatcttatg taccaaaagt aatctactaa aagtatacag cggtttcggc gccagtgaca
     1141 ggagttttca tgcatgtcct tatcgtgacc gcccgaccgg gagaggcgtc gctgacacag
     1201 gcgctcggcc gggccgtggc cgacggcgtc gaggcggccg gtgcgaccgc cgagatcgcc
     1261 gatctcgctt ctgaaggttt cgacccccgc ttcaccgccc gggatatcga ggttcatctt
     1321 gccacggccg tgcccgatgc ggatgtgacg gcggagcagc ggcggctgga tcgcgccgat
     1381 gcgctcgttc tcgtctatcc gatctattgg tggtcctttc ccggactgct caagggctgg
     1441 gtcgaccggg tcttctgccg ggggtgggcc tatgaggtca acgaggccga cgccgtcatc
     1501 gaaaagcggc tcacgaacct gacggtccac ctcgtggctg tcggcggagc gggcctcaga
     1561 acctatgccc ggcatgggta tttcggagcc atgaagcttc agatcgatca cggcgtcttc
     1621 gactactgtg gtgcgacggt cgccacctcc gaactgatgg cggatcccgt tcccgaggcg
     1681 gctctcgagc acgcccgggc gatcgggcgc cgcgccgccc tggtcagggg cgccttgcac
     1741 agagccgcct gacgcgagcc cgcggccggc gggcgatcat ggatgttcgc gccggccacg
     1801 tctcgccgtc cgccgacggc gtcttgcgcg aagcgcgccg ttttcgcccc gtgcgccggg
     1861 accgattgcc agggaggggc tggacgacgg cgccggcggg agccgcggaa ggggcgcaag
     1921 gcggaccgga agcttcagtc gtcagggatt tgcggcggat tgaggccgcg ccggatcaga
     1981 cccaagtcgg ggggagccgg gggccgaacg ccggatcgtc gtcctggtgc tcgcggtgat
     2041 aggtcagcag tctgcccagt tcgtcccggc ccaggccgcc tgagcgatcg gaatccacg
//
LOCUS       DASVVN010000269         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_168090,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000269 DASVVN010000000
VERSION     DASVVN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168090"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..312)
                     /locus_tag="VF686_04255"
     CDS             complement(115..312)
                     /locus_tag="VF686_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661056.1"
                     /translation="MKIPPESRSFLDEEMEAAAEALKWQAASARAAGAKHRARLIDQL
                     DAQAPDTTPAEDDSSEISPAA"
     gene            472..>1609
                     /locus_tag="VF686_04260"
     CDS             472..>1609
                     /locus_tag="VF686_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661057.1"
                     /translation="MANRVLIIGAGHAGGTAAALLRQYGHEGPIVLAGEEPAAPYQRP
                     PLSKAWLKGEADLEALLLRPESFYEEHGIELRTGVTATAIDPAARTVSFADGTVEPWD
                     ALILATGSRARRLTIPGAERPELMELRTLADAERLKAALGPGRRLAIVGGGYVGLEAA
                     ASARALGAEAVVIERMDRVLARVASETLSAFFTARHRGEGVEVLTGAEVTAFEDAGVR
                     LADGRLIDADAVLIGVGALACDELARTAGLDCGNGVVVDEHARTSDPAIWAIGDATVR
                     PIPVHGGRRHRLESVPNALEQAKQAASAIVGRAPPTPEVPWFWSDQYDLKLQIAGLPD
                     GADRQIVRGDPRVTGEGASFAVFHLEGDRILCVEAVNAPAEFMAG"
BASE COUNT          228 a          501 c          627 g          253 t
ORIGIN      
        1 gtgcgcagcg acaggatcgc gtcatacaag gcgacgcgtt cgtctgcagc ctttgcggcg
       61 gccttctgcg aggggttgat catgacgttc cactaagctg taacactgga acagtcaagc
      121 ggcggggctg atctccgacg aatcgtcttc ggcaggggta gtatccgggg cttgggcgtc
      181 cagttggtcg ataaggcggg cgcggtgttt ggctccggcg gcgcgagcgg aggcggcctg
      241 ccatttcagc gcctcggccg cggcctccat ctcttcgtcg aggaaggagc gggactcggg
      301 agggatcttc atcgggactg actaactacg gcccgatcaa agtcgggtac cgttcacaac
      361 ccgggcctgc ctgattggtt tccgggagac tcggggtttt agggcgggtg tgtttcccct
      421 acctcacccg gggcggctgc ggccgtccgg aaaacgggat acagggcctg aatggcgaac
      481 agggtgctca tcatcggcgc gggacacgcg gggggaacgg cggcggcgct gctgcgccag
      541 tacggccatg agggcccgat cgtgctggcg ggcgaggagc cggcggcgcc ctatcagcgc
      601 ccgcccctgt ccaaggcctg gctgaaaggc gaggcggatc tggaggcgct gctgctgcgc
      661 cccgaaagct tctacgagga acacgggatc gagctgcgca cgggcgtgac ggcgaccgcg
      721 atcgatccgg cggcgcggac cgtcagcttc gccgacggga cggtcgagcc ttgggacgcg
      781 ctgatcctgg cgacgggttc gcgggcgcgg cggctgacga tcccgggcgc ggagaggccg
      841 gagctgatgg agctgcggac gctggccgac gccgagcggc tgaaggcggc gctggggccg
      901 ggccgccggc tggccatcgt cggcggcggc tatgtcgggt tggaggcggc ggcgtcggcg
      961 cgggcgctcg gagcggaggc agtcgtgatc gaacggatgg accgggtgct ggcgcgcgtg
     1021 gcttcggaga ccctgtcggc cttcttcacc gcccgtcatc gcggcgaggg cgtcgaggtc
     1081 ctgaccggcg ccgaggtcac ggcgttcgag gacgcgggcg tgcggctggc cgacggccgg
     1141 ctgatcgatg ccgatgcggt gctgatcggc gtaggcgcgc tcgcttgtga cgagctggcg
     1201 cgaacggcgg gtctggactg cggcaacggg gtggtcgtgg acgaacacgc gcggacgtcg
     1261 gatcccgcga tctgggctat cggtgacgcg accgtccggc cgatccctgt ccacggcggg
     1321 cggcggcatc gtctggagag cgtgcccaat gcgttggagc aggccaaaca ggcggcctcg
     1381 gccatcgtcg gccgcgcacc gccgacaccc gaggttccgt ggttctggtc ggaccagtac
     1441 gacctcaagc ttcagatcgc cggcctgccc gacggcgcgg atcgccagat cgtgcgtggc
     1501 gacccccgcg tcactggcga gggggcctcg ttcgcggtct tccacctcga gggcgaccgc
     1561 atcctgtgcg tcgaggccgt caacgcaccg gccgaattca tggccggac
//
LOCUS       DASVVN010000270         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_62237, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000270 DASVVN010000000
VERSION     DASVVN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62237"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VF686_04265"
     CDS             complement(<1..535)
                     /locus_tag="VF686_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8661058.1"
                     /translation="MFAIWMDVLLSVVARRTWTASNRCDDGRWISRGLFGDVSSIYSN
                     DQGAVLNLNDLQAFVQVVDHGGFTAAGRALGVPKSTLSKRLSDLETAIGVRLVQRTSR
                     SFAVTEIGRDFYRHAAAMLIEAEAAENVVKGRLAEPSGTVRITASLPIAQFRLASLLP
                     RLAASFPKVRIMLDVSDR"
     gene            525..1403
                     /locus_tag="VF686_04270"
     CDS             525..1403
                     /locus_tag="VF686_04270"
                     /inference="COORDINATES: protein motif:HMM:NF044865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8661059.1"
                     /translation="MANISFLGLGAMGQAMTARLLEQGHAVTVWNRTPGKAAALVAKG
                     AREVHSPAEAIAASALTIMCVLDYAASDSVIDSAVSDLSGRTLVNLTNGTPAQARAAA
                     ERAGALGAAYLDGGIMATPPMIGGEHALILYSGSQAAFDAHNPTLRALGEADYLGTDA
                     GLASLHDLALLSGMYGVFAGFFHATALVASEGVAAGAFLKMLAPWMTAMMGELPGYAQ
                     KIDSGRHDENVVSNLGMQTASLDNIVTATREQGVASDLLDPMLELFRARSAAGRGSED
                     ISGIFELIRRGAGANA"
     gene            1491..2177
                     /locus_tag="VF686_04275"
     CDS             1491..2177
                     /locus_tag="VF686_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8661060.1"
                     /translation="MPPLHLLHIDSSASLGAYGIDPHGSHTRRLTRHVIQRWRALRPE
                     DQVTYRDVSRQPPSPVTEDWIEAAFTPPERRAAAMIARLAESDRMIEELEAADVVVVG
                     APMYNYGIPSPLKAWIDNVVRVGVTFGFDRERSGGHYWPMLSAGKRLLILSSRGEGGY
                     DDGGPLAPLNLVERQIEAAMAFVGLGEAHRISIEHVDVGDESLLAASVARAEVDIDSL
                     VRRLASAPVP"
BASE COUNT          408 a          862 c          832 g          386 t
ORIGIN      
        1 acctgtcgga gacatccagc atgatgcgga ccttgggaaa gctcgcggca agccggggga
       61 ggagcgaagc caggcggaac tgggcgatcg gcagggatgc ggtgatccgc acggttccgc
      121 tcggctcggc gaggcgcccc ttgacgacat tctccgccgc ctccgcctcg atcagcatcg
      181 cggcggcgtg acggtagaag tcgcggccga tttcggtcac cgcgaaactg cgtgaggtcc
      241 gttgaaccag gcgcacgccg atggccgtct ccaggtcgga cagccgcttg ctgagcgtgg
      301 atttcggaac gcccagggcg cgcccggcgg ccgtgaagcc gccgtgatcg accacctgaa
      361 cgaaagcctg caagtcgttg agattcagca ctgcgccttg atcgttcgaa tatatggacg
      421 atacgtcccc aaatagccct ctactgatcc accgtccatc gtcgcatctg ttgctcgccg
      481 tccaggtgcg gcgcgcaaca acagacagaa ggacgtccat ccatatggcg aacatctcgt
      541 tcctcggcct gggggccatg ggccaggcca tgaccgcccg cctcctcgag cagggccacg
      601 ccgttacggt gtggaaccgc acgcccggca aggccgccgc ccttgtcgcc aaaggcgcgc
      661 gtgaggtgca ttcgcccgca gaggccatcg ccgccagcgc cctgaccatc atgtgcgttc
      721 tggactatgc cgcgtcggat tccgttatcg acagcgccgt ctcggacctt tccgggcgta
      781 cgctggtcaa tctcaccaac ggaacgccgg cgcaggcgcg cgccgccgcc gagcgggcgg
      841 gcgccttggg cgccgcctat ctcgacggcg ggatcatggc gacgccgccg atgatcggcg
      901 gtgagcatgc gctcatcctt tacagcggct cgcaggcggc cttcgacgcc cacaatccga
      961 cgctgagggc tctgggcgag gcggactatc tggggacgga cgccggcctg gcgtccttgc
     1021 acgatctggc gcttctcagc ggtatgtacg gcgtgttcgc gggcttcttc cacgcgacgg
     1081 cgctcgtcgc gtccgagggc gtcgccgccg gcgcgtttct caagatgctg gccccgtgga
     1141 tgaccgcgat gatgggcgag ctgccgggct atgcgcaaaa gatcgacagc ggaaggcacg
     1201 acgagaacgt cgtgtcgaac ctcgggatgc agaccgcttc gctggacaac atcgtcaccg
     1261 ccacccgcga gcagggggtc gccagcgacc tgctggatcc catgctggag ctgttccgcg
     1321 cgcgctccgc cgccggccgc ggcagcgagg acatctcggg catattcgag ctgatccggc
     1381 gcggggcagg cgcaaacgcc tgagccccgg ccccgccgtc cgcccgcccg cccgcggatc
     1441 tcacgcgccg caacgcccca ttgaatgatt ttcgacagga gagcctccgc ttgccccccc
     1501 tgcacctgct gcacatcgac tcgagcgcaa gcctgggcgc ctatgggatc gatccgcatg
     1561 gttcgcacac ccggcgcctg acgcggcacg tcatccaacg ctggcgcgcc ttgcggccgg
     1621 aggatcaggt gacatatcgc gatgtgagcc gccaaccgcc gtcgcccgtc accgaagact
     1681 ggattgaagc ggccttcacg cctccggaac gacgggcggc cgcgatgatc gcccgcctcg
     1741 ccgagagcga tcgcatgatc gaagaactcg aggcggccga tgtcgtggtg gtgggcgcgc
     1801 cgatgtacaa ctacggcatt ccctccccgc tcaaggcctg gatcgacaac gtcgtccggg
     1861 tcggcgtcac attcgggttc gatcgggagc ggagcggtgg ccactactgg ccgatgctgt
     1921 ccgccggcaa gcggcttctc attctctcgt cgcgtggcga aggcggctat gacgacggcg
     1981 ggccgctggc acccctgaac ctggtggaac gccagattga ggctgccatg gccttcgtcg
     2041 gtctgggcga ggcgcaccgg atctcgatcg agcatgtcga cgtcggcgac gagagcctgc
     2101 tggcggcctc tgtcgcaagg gctgaagtcg acatcgacag cctggtgcgc aggctcgcct
     2161 ccgcgccggt tccctgacgc atgtctcaag acgccgcagt tccgcctgcg cgcaaccgcc
     2221 agggccggcg gatcatagcg caggggcgcg cgcagcaagg ggacggcgga tcgccgcgcg
     2281 ccggggcgcg gctgacggcc gtgatcaggg agtcatcgca atcccgccaa acttgtcact
     2341 cccccggagc cggctgatgg ccccacgatc gcaatcccca ggaggggccg cccgccacgc
     2401 aggccgggcc gctcccagcc cgcacggaga tagcgatgca cgagatccag tcacagattc
     2461 aggcccggga cccggagctg gccgggct
//
LOCUS       DASVVN010000271         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_141859,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000271 DASVVN010000000
VERSION     DASVVN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141859"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF686_04280"
     CDS             complement(<1..237)
                     /locus_tag="VF686_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HEX8661061.1"
                     /translation="MIEVEIEDAAWTTDLPDADAVAERAARAALGRVEGDVVVLLTDD
                     EGVRDLNARFRDKDRPTNVLSFPAAESAAPHLGDL"
     gene            complement(234..1229)
                     /locus_tag="VF686_04285"
     CDS             complement(234..1229)
                     /locus_tag="VF686_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX8661062.1"
                     /translation="MARESEFLALGDEALRAVIGPGSRHLALIEDRFKVLVETPGGGV
                     SINGSSKDRSQAKRVIETLANRSETGAEITEADVRTALGAAATQPRAGQGSVAPDAIA
                     LPVGRRGAIAPKTAAQANYLALLSQCELTFGVGPAGTGKTFLAAAYGASLLRRGQVDR
                     LVITRPAVEAGEKLGFLPGDLNEKVDPYLAPIWEALNDILGPDDVRRRRDKGEIEAAP
                     IAFMRGRTLAHAYIIVDEAQNTSRLQMKMVLTRLGEGARMVVTGDPSQVDLLNPRDSG
                     LKHALRILEDVKGVGVQRFQAQDVVRHAMVERIVRAYDADAAARRPAADLEDPDA"
     gene            complement(1219..>1922)
                     /locus_tag="VF686_04290"
     CDS             complement(1219..>1922)
                     /locus_tag="VF686_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8661063.1"
                     /translation="YALAEIPGIDRIRYTTSHPNDMSDALIAAHGELDALMPWLHLPV
                     QAGSDRVLRLMNRKHGRAKYLDLVERIRDSRPDIAMAGDFIVGFPGETDRDFEDTMDL
                     VRRVNYASAFAFMYSPRPGTPAATMAGQVSTEASRERLHALQALLAEQQLAFNQDKAG
                     RTFDVLFEKKGRHGAQAVGRSPWLQSVHVDDADHLIGRIVPVTIEYGQQNSLKGRLIH
                     ERATPLQQRDQQIGA"
BASE COUNT          275 a          699 c          636 g          312 t
ORIGIN      
        1 cagatcgccc agatgcggcg cggcgctctc cgccgccggg aaggacagca catttgtggg
       61 ccgatccttg tcgcggaagc gggcgttcag atcacgcacg ccttcgtcgt cggtcagcag
      121 caccacgaca tcgccctcga cccggcccag tgcggccctc gccgcccgtt cggccacggc
      181 gtcggcgtcc ggcaggtcgg tcgtccaggc cgcgtcctcg atctcgacct cgatcatgcg
      241 tccggatctt ccagatcggc ggcggggcgg cgggcggccg cgtcggcgtc gtaggcgcgc
      301 acgatccgct cgaccatggc gtggcgcacc acgtcctggg cctggaagcg ctgcaccccg
      361 acgcccttca catcctcgag gatgcgcagg gcgtgcttca gccccgagtc gcgcgggttc
      421 agcagatcga cctgcgacgg gtcgcccgtc accaccatcc gcgcgccctc gcccagccgg
      481 gtcagaacca tcttcatctg cagccgcgag gtgttctggg cctcgtcgac gatgatgtag
      541 gcatgggcca gggtccggcc gcgcatgaag gcgatcggcg ccgcctcgat ctcgcccttg
      601 tcgcgccggc ggcgcacgtc gtcggggccg aggatgtcgt tcagcgcctc ccagatgggc
      661 gccagatagg ggtcgacctt ctcgttcagg tcgccgggca ggaagcccag tttctcgccc
      721 gcctcgaccg ccggccgggt gatgaccagc cggtccacct gcccccgccg cagcagcgat
      781 gcaccgtagg ccgccgccag aaatgtcttg cccgtgcccg cggggcccac gccgaacgtc
      841 agctcgcact gcgatagcag cgccagatag ttggcctggg ccgccgtctt gggcgcgatc
      901 gccccgcgcc gcccgaccgg cagggcgatg gcgtccgggg ccacgctccc ctgccctgcc
      961 cgcggctggg tcgccgccgc gcccagcgcc gtgcgcacgt cggcctcggt gatctcggcg
     1021 cccgtttccg agcggttggc caaggtctcg atgacccttt tcgcctgcga ccggtccttc
     1081 gaggacccat tgatcgacac gcctccgccg ggcgtctcga ccagaacctt gaaccggtcc
     1141 tcgatcaggg ccaggtggcg gctgccggga ccgatgacgg cgcgaagggc ttcgtcgccc
     1201 agggccagaa actcactctc acgcgccaat ctgttggtcc cgctgctgga ggggcgtcgc
     1261 ccgctcgtgg ataagccggc ccttcagcga attctgctgg ccgtattcga tcgtcaccgg
     1321 aacgatccgg ccgatcagat gatccgcgtc atcgacgtgg accgattgca gccacggcga
     1381 acgcccgacg gcctgggccc cgtgacggcc cttcttctcg aacaggacgt cgaaggtccg
     1441 gcctgccttg tcctgattga acgccagctg ctgctcggcc agcagcgcct gaagggcatg
     1501 cagccgctcg cgcgaggcct cggtcgaaac ctgccccgcc atggtcgcgg ccggcgtgcc
     1561 cgggcgcggc gaatacatga aggcgaaggc cgaggcgtag ttcacccgcc gcaccagatc
     1621 catcgtgtcc tcgaaatccc gatccgtctc tcccgggaaa ccgacgatga agtctcccgc
     1681 catggcgata tcgggccggc tatcgcggat gcgctcgacc agatcgaggt atttcgcacg
     1741 tccgtgcttg cggttcatca gccgtagcac ccggtccgat cccgcctgca caggcaggtg
     1801 caaccacggc atcagggcgt ccagttcgcc atgggcggcg atcagcgcgt cggacatgtc
     1861 gttggggtgg ctggtcgtat agcggatgcg gtcgatgccg gggatctccg ccagcgcata
     1921 gg
//
LOCUS       DASVVN010000272         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_457752,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000272 DASVVN010000000
VERSION     DASVVN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457752"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1562
                     /locus_tag="VF686_04295"
     CDS             150..1562
                     /locus_tag="VF686_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8661064.1"
                     /translation="MRLMTGAAVLSAAILSACTQNEKPQTASAQTPRTPPKTYSRDPF
                     PSTYRRYPGPVTAITGATVFDGEGARIDNGTVVLADGLVQAIGGPDTAIPDGATRIDA
                     AGKWVTPGIIDIHSHLGDYPSPSVEAHQDGNEVTSPVRPEVWAEHSVWPQDPGFSRAL
                     VNGGVTSLHVLPGSANLFGGRGVTLKNVPGRTMQQMKFPGAPYSLKMACGENPKRVYG
                     GRNQMPGSRMANVALMRQTWARAQEYRRKWDAYERNGGEMPGRDLAMDTLRGVLAGEI
                     TVQNHCYRADEMANIMDMAREFGYKVASFHHAVESYKIADLLKAHGACSAMWADWYGF
                     KMEAYDGIQENIPLVHNAGACAIVHSDDANGIQRLYQEAAKALANGRRMGIQISDETA
                     WTWLGINPARALGIGDRTGSLRPGKMADVVLWNGHPFSVYTRPERVWVDGALMYDSND
                     PRRRPVSDFELGQPGEGDVK"
     gene            1676..>1887
                     /locus_tag="VF686_04300"
     CDS             1676..>1887
                     /locus_tag="VF686_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX8661065.1"
                     /translation="MAALLAFATPALAQTIAITGGTVALGDGSEPIEGGTVVVQNGRI
                     LAAGRNVAVPAGAQRVDATGKWVTPG"
BASE COUNT          348 a          645 c          634 g          260 t
ORIGIN      
        1 tcatgcatct cgacacgctc cgcgacaacg aggacgagga gctcgccggc tacaaggagg
       61 tcggagggga ccggggccag gacgccggaa tgttccctca ccgcgaaacc aagcctgaaa
      121 ccaaagagac ctgacgaaaa aggacttcga tgcgccttat gaccggagcg gccgtcctct
      181 cggccgccat cctttccgcc tgtacccaga atgagaagcc gcagacggcg tccgcccaga
      241 cgccccggac tccgcccaag acctattcga gggatccctt cccctcgacc taccggcgct
      301 atccgggccc ggtgacggcg atcaccggcg cgacggtgtt cgacggcgag ggcgcccgga
      361 tcgacaatgg cacggtggtg ctggccgacg gcctcgtcca ggcgatcggc ggcccggaca
      421 cggccattcc cgacggcgcg acccggatcg acgcggccgg caaatgggtg accccgggga
      481 tcatcgacat ccacagccac ctcggcgact atccgagccc ctcggtcgag gcgcatcagg
      541 acggcaacga ggtgaccagc ccggtccggc cggaagtgtg ggcagagcac agcgtctggc
      601 cgcaggaccc gggcttcagc cgggcgctgg tcaacggcgg ggtgaccagc ctccacgtcc
      661 ttcccggctc ggccaacctg ttcggcggcc gcggcgtgac cttgaagaac gtccccggcc
      721 ggacgatgca gcagatgaag ttccccggag cgccttattc gctgaagatg gcctgcgggg
      781 agaaccccaa gcgcgtctat ggcggccgca accagatgcc gggcagccgg atggcgaacg
      841 tcgcactgat gcgtcagacc tgggcccggg ctcaggaata tcggcgcaaa tgggacgcct
      901 acgagcgcaa cggcggcgag atgcccggcc gcgacctcgc catggacacg ctgcgtggag
      961 tgctggccgg cgagatcacg gtccagaacc attgctaccg cgccgacgag atggccaata
     1021 tcatggatat ggcccgggag ttcggctaca aggtggcgag cttccaccat gccgtcgaga
     1081 gctacaagat cgccgatctg ctgaaggcgc acggcgcctg ctcggcgatg tgggccgact
     1141 ggtacggctt caagatggaa gcctatgacg gcatccagga gaacatcccg ctggtccaca
     1201 atgccggcgc ctgcgcgatc gtccattccg acgacgccaa cggcatccag cgcctctacc
     1261 aggaggcggc caaggcgctc gccaacgggc ggcggatggg aatccagatc tcggacgaga
     1321 ccgcctggac ctggctgggc atcaacccgg ccagggcgct cggcatcggc gacaggacgg
     1381 gcagcctgcg ccccggcaag atggccgacg tggtgctctg gaacggccac ccgttcagcg
     1441 tctacacgag gcccgaacgg gtctgggtgg acggagccct gatgtacgat tccaacgacc
     1501 cgaggcgccg cccggtgagc gatttcgagc tcggccagcc gggcgaaggg gatgtgaagt
     1561 gaagggcgtg ttccaaaccc gtcattccgg acttgatccg gaatccatga acaccgccgc
     1621 cgggcgttcc gtacgaccgc gttcatggat cccggccttc gccgggatga cgtgtgtggc
     1681 tgcgctcctc gccttcgcca cccccgccct tgcccagacc atcgccatca ccggcggcac
     1741 agtcgcgctc ggcgacggct ccgagccgat cgagggcggc accgtcgtcg ttcagaacgg
     1801 gaggatcctc gcagcgggcc gcaacgtcgc ggtgccggcg ggcgcccagc gggtcgacgc
     1861 gaccggcaaa tgggtcaccc ctgggat
//
LOCUS       DASVVN010000273         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_141943,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000273 DASVVN010000000
VERSION     DASVVN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141943"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF686_04305"
     CDS             complement(<1..243)
                     /locus_tag="VF686_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system subunit TolQ"
                     /protein_id="HEX8661066.1"
                     /translation="MTTAAEASMINPVTLFLTADIVVQAIMVGLVIASLWSWAVIIDK
                     AVRFASLNSQANNFEQAVGSGRSLEEVAAQAGPNPAH"
     gene            complement(383..829)
                     /gene="ybgC"
                     /locus_tag="VF686_04310"
     CDS             complement(383..829)
                     /gene="ybgC"
                     /locus_tag="VF686_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX8661067.1"
                     /translation="MTDLPATGRFEGREHRLPVRVYYEDTDFTGLVYHANYVRYFERG
                     RSDCLRLMGIGHADLLDGDQPMAFVVTKMQLNFLRPARIDDALIVRTHYDAVNGPRLL
                     ISQSVTRGEDVLCRADVEVVCIHMDGRPRRPTRTLVEKIGPWLREA"
     gene            complement(826..1278)
                     /locus_tag="VF686_04315"
     CDS             complement(826..1278)
                     /locus_tag="VF686_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661068.1"
                     /translation="MAANPQAGFTSGPNPGMVAAEGDGMARRAIRTERLIAVLGAVVL
                     LLGALILWKTIEASRPGFTEEAPKRRVLRQAREQLGRTAEIRLLDEGRGRVVCGYVGR
                     RGESGAWSFISRPNRIMMSDDPLATEFQAMLRADCPHLPKPPPLGAVP"
     gene            1320..2864
                     /locus_tag="VF686_04320"
     CDS             1320..2864
                     /locus_tag="VF686_04320"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8661069.1"
                     /translation="MRRNALVMTVLLMGAGAALPVQAQTPPAAAAAAATPASLHEAIP
                     VIDAAFDRWQANAHAPGLVYGVVQNGEIVHLRTTGVRTVDGAGVTPDTLFRIASMSKA
                     FTALAILKLRDEGKLSLDALAETYVPELADWTYPTADSPRIRVRDLLNHTGGFVTDDP
                     WGDRQQVLTEAEFTRMLEDGVPFTRAPQTAFEYSNFGYALLGRIVTNVSGQPYDQYIE
                     AEIMRPLGMTSSGYDVFAAPQDRRALGYRWENDAWSREPDMRHGVFGSMGGVQTSAQD
                     YARWIAFLLSAWPARDGPETGPVRRSTVREMAQGSNFAAPRGRTGADGQPCPGSSAYG
                     MGLQANVDCELSFYLAHGGGYPGYGSFMMLMPERGTGLFAFSNRTYAGPSAPLIEAAS
                     TLVRGGLAPVRTPEASAILTEHYADLGRIWTVGDVSVANDRLAMNFLMDRSVENWRTV
                     LGQLKGDAGACATEAPVTATGGLSGTFRWTCDKGVVEGRLLLAPTTPVTIQAINFRFT
                     PTPAPQ"
     gene            complement(3113..>3450)
                     /locus_tag="VF686_04325"
     CDS             complement(3113..>3450)
                     /locus_tag="VF686_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661070.1"
                     /translation="FAGDGKIDKLSAARALARLEVDEAGLDANDRRFLKALIENYGGG
                     PVGMDTLAAAIAEARDAVEDVIEPYLLQQGFIQRTPRGRMACAKAYAHLGLTEPPKPA
                     GTGQAALFE"
BASE COUNT          505 a         1197 c         1184 g          564 t
ORIGIN      
        1 atgggcgggg ttcggccccg cctgggcggc gacctcctcc agcgaccggc ccgagccgac
       61 ggcctgttcg aaattgttcg cctggctgtt cagcgaggcg aaccgcaccg ccttgtcgat
      121 gatcacggcc cacgaccaca gcgaggctat gaccaggccg accatgatcg cctgcacgac
      181 gatgtcggct gtcaggaaca gggtcacggg gttgatcatc gaggcttcgg cggcggtggt
      241 catcggcatt cctggcgtct gtccatccct gtgcaccgga cgggcgtggc ggttctgtgg
      301 cggtcggacc gggcgcgaat ccggttgtgc cgaggtgtag ccacgacgtc acgcgtctgc
      361 acgtgacagg ttcgcaatgt tgtcaggctt cgcgcagcca gggtccgatc ttttcgacca
      421 aggttctggt cggccgcctg ggccggccgt ccatatggat gcagaccacc tcgacatcgg
      481 cgcggcacag cacgtcctcg ccccgcgtga cggattgcga gatcagcagc cgcggcccgt
      541 tcacggcgtc atagtgggtc cgcacgatca gggcgtcgtc gatccgcgcc ggcctgagga
      601 agttcagctg catcttcgtg acgacgaagg ccatgggctg gtcgccgtcc agcaggtcgg
      661 cgtggccgat tcccatcagc cgcagacagt cggaccgccc ccgctcgaaa tagcggacgt
      721 agttggcgtg atagaccagt cccgtgaagt cggtgtcctc ataatagacc cggacgggca
      781 gacggtgttc gcggccttcg aagcggccgg tggcaggcag gtccgtcatg ggacggcgcc
      841 gagcggcggc ggcttgggca ggtgcggaca gtcggcgcgc agcatggcct ggaattcggt
      901 cgccagaggg tcgtcgctca tcatgatccg gttgggtcgc gagatgaaac tccaggcgcc
      961 gctctcgccg cgccgcccca catagccgca gacgacgcgg ccgcgcccct cgtccagcag
     1021 ccggatttcg gcggtgcggc ccagctgctc gcgggcctga cgcagcaccc ggcgtttggg
     1081 ggcctcttcg gtgaagccgg ggcgcgacgc ctcgatcgtc ttccacagga tcaaggcgcc
     1141 cagcaacagc accacggcgc ccagcaccgc gatcagccgc tcggttcgga tcgcccttcg
     1201 cgccatgccg tctccttcgg ctgcgaccat gccggggttc ggcccggagg tgaagcccgc
     1261 ctgcggattg gccgccacgc cgcccgatgc taggtctcga tcacaatcgg gaggggatca
     1321 tgcgacgcaa cgctctggtc atgacggttc tgctcatggg ggcgggggcc gcgctcccgg
     1381 ttcaggcgca gacgccgccc gccgccgccg ccgccgccgc gacgccggcc agccttcatg
     1441 aggcgatccc cgtcatcgac gccgccttcg accgctggca ggccaacgcc catgcgccgg
     1501 ggctggtgta tggcgtcgtc cagaacggcg agatcgtcca tctgcggaca accggcgtgc
     1561 ggaccgtcga cggcgccggg gtgacgcctg acaccctttt ccggatcgcc tccatgtcca
     1621 aggccttcac cgccctggcg atcctgaagc tgcgcgacga aggcaaactg tcgctggacg
     1681 ccctggccga gacctatgtg ccggagctgg cggactggac gtatccaacc gccgattccc
     1741 cgcgaatccg ggttcgcgac ctgctcaacc ataccggcgg tttcgtcacg gacgatccct
     1801 ggggcgaccg ccagcaggtc ctgaccgagg ccgagttcac tcgcatgctc gaggatggcg
     1861 tgcccttcac ccgcgcgccg cagacggcgt tcgaatattc caatttcggc tacgccctgc
     1921 tcggccggat cgtcaccaat gtctcgggcc agccctacga ccaatatatc gaggccgaga
     1981 tcatgcggcc gctggggatg acgtccagcg gctatgacgt cttcgccgcg ccgcaggatc
     2041 gccgcgcgct cggctatcgc tgggagaatg acgcctggtc gcgcgaaccg gacatgcggc
     2101 acggcgtgtt cggatcgatg ggcggggtgc agaccagcgc gcaggactac gcccgctgga
     2161 tcgccttcct gctgtcggcc tggcccgccc gggacggccc ggagacggga ccggtccgcc
     2221 gctccacggt gcgcgaaatg gcccagggct cgaatttcgc cgcgccgcgc gggcgcaccg
     2281 gcgccgacgg ccagccctgt ccggggtcga gcgcctacgg catgggcctg caagccaacg
     2341 tcgactgcga gctgagcttc tatctggcgc acggcggcgg ctaccccggc tacggctcct
     2401 tcatgatgct gatgccggag cgggggacgg gcctgttcgc cttctccaac cgcacctacg
     2461 ccggtccgtc ggccccgctg atcgaggccg cctcgacgct ggtgcgcggt gggctggccc
     2521 cggtccggac gccggaggcc agcgccatcc tgaccgaaca ctacgccgac ctcggccgca
     2581 tctggaccgt cggggacgtg agcgtcgcga acgaccggct ggcgatgaat ttcctcatgg
     2641 accgctcggt cgagaactgg cgcaccgtgc tgggccagtt gaagggcgac gccggcgcct
     2701 gcgccacgga ggcgccggtc accgcgacgg gcggcctgtc ggggactttc cgctggacct
     2761 gcgacaaggg cgtggtcgag gggcggctgc tgctggcgcc gacgacgccg gttacgatcc
     2821 aggcgatcaa tttccgcttc acgccgacgc cggccccgca gtagttcaga ggtctccaag
     2881 gcttggcgac ttgtcatccc ggacgccgcg cagcggtgat ccgggacgct gagtcgtccc
     2941 attttcatcc tcccggaagc tgcgccagca gctatccggg tgacgggtca ggatgctgcg
     3001 ggcggctgcc tgtttcccgg ataatcgcgt cgcgatttcc gggaggttga ggtcgatggt
     3061 caactccgtc ccggatcgcc gcgtcgcggc gtccgggatg acaaacacct ccctattcga
     3121 acagggccgc ctgtccggtc cctgccggtt tcggcggctc ggtcaggccg aggtgggcat
     3181 aggccttggc gcaggccatg cgtccgcgcg gcgtccgctg gatgaagccc tgctgcagca
     3241 gatagggctc gatcacgtcc tcgacggcgt cgcgcgcctc ggcgatggcg gccgccaggg
     3301 tgtccatgcc gacggggccg ccgccgtagt tctcgatcag ggccttgagg aagcggcggt
     3361 cattggcgtc cagaccggcc tcgtcgacct ccagccgcgc cagcgcccgc gccgccgaca
     3421 gcttgtcgat cttgccgtca ccggcgaaat
//
LOCUS       DASVVN010000274         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_273033,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000274 DASVVN010000000
VERSION     DASVVN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273033"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF686_04330"
     CDS             <1..206
                     /locus_tag="VF686_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661071.1"
                     /translation="AASGPATTGTPGEVGFGQLLQGYTEASNVDAVTEISSLIIAQRA
                     YEMNSKVITTADQMLSVAGQVKS"
     gene            203..808
                     /locus_tag="VF686_04335"
     CDS             203..808
                     /locus_tag="VF686_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEX8661072.1"
                     /translation="MRTLILAALAALAFAGPALAGPVTLKVNPMDSDGRVTLGDLFDG
                     AGAASSVVVGQRAGPSIVFEAGQLQSLARQSGLDWANPTGLRRVVVRNAAMAPGASAV
                     AAADAAVGTAGPAVSRPAPVRATHGERVISRNDIVEVAYEAGGVRLTISGRAEGNAAV
                     GQRLAVRNLQSNRVIDAVATAPGQAAAGPAARASTQQLAAR"
     gene            823..1563
                     /gene="flgH"
                     /locus_tag="VF686_04340"
     CDS             823..1563
                     /gene="flgH"
                     /locus_tag="VF686_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX8661073.1"
                     /translation="MRTAMILTAAVSCLTLGACSTAVETVMGPQLAPIGYPSALVPTD
                     QIVMASAAQQQADRAASANSLWRTGARTFFGDQRARSIGDILTVNIDIDDRAQTQNST
                     NRSRTNDISGGISNFFGLENSLGRAFPGGFDPQNLVGLEGESNATGSGSVNRSEKVSL
                     TIAAVVTGVMANGNLVIQGRQEVRTNREVRELTVAGIVRPEDISSGNTIAHTQIAEAR
                     ISYGGRGDISRVQATPAAQALVERFSPF"
     gene            1726..>2028
                     /locus_tag="VF686_04345"
     CDS             1726..>2028
                     /locus_tag="VF686_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661074.1"
                     /translation="MLKFAIPAVAALGLIAFAAQSQATNSDARTGAAAPMAWHLSHEG
                     DLAKLAYGVANSDQLALMLTCEPAQAQAVVYGDVKPAGPRVQKASMRAVAIDPLSGG"
BASE COUNT          321 a          763 c          655 g          289 t
ORIGIN      
        1 cggcggcctc gggccctgcc accaccggca cgcccggcga ggtcggcttc ggccagctgc
       61 tgcagggcta taccgaggcg tcgaacgtcg acgcagtgac cgagatcagc tcgctgatca
      121 tcgcccagcg cgcctacgaa atgaactcga aggtcatcac caccgccgac cagatgctgt
      181 cggtcgccgg gcaggtgaag tcgtgaggac cctgatcctc gccgccctcg cggccctggc
      241 gttcgcggga cccgccctcg ccggtccagt gacgcttaag gtcaatccga tggattcgga
      301 cggccgcgtg accctgggcg atctgttcga cggcgccggg gcggcgtcca gcgtcgtcgt
      361 gggccagcgg gccggcccct ccatcgtgtt cgaggccgga cagctgcaga gcttggcccg
      421 ccagtccggc ctggactggg caaatccgac cgggctgcgc cgggtcgtgg tgcgcaacgc
      481 cgccatggcg cccggcgctt ccgccgtcgc cgctgcggat gcagccgtcg gaaccgccgg
      541 ccccgccgtt tcgcgtcccg ccccggtgcg cgcgacccac ggtgaacgcg tcatcagccg
      601 caacgacatc gtcgaggtcg cctatgaggc cggcggcgtc cgcctgacca tcagcggccg
      661 ggccgagggc aacgccgccg tcggccagcg tctggcggtc cgcaacctgc aatccaaccg
      721 ggtcatcgac gccgtcgcca ccgcgcccgg tcaggccgcc gccggccccg ccgcccgcgc
      781 ctccacccaa caactcgccg cccgctgagg ggaaacagtc ttatgcgtac cgccatgatc
      841 ctgaccgccg ccgtgtcctg cctgacgctg ggcgcctgtt cgacggccgt cgaaaccgtc
      901 atggggcctc aactggcccc gataggctat ccctcggcgc tggtcccgac cgaccagatc
      961 gttatggctt ccgccgccca gcaacaggcg gaccgggccg ccagcgccaa cagcctgtgg
     1021 cgcacgggcg cccgcacctt cttcggtgac cagcgtgcgc gcagcatcgg cgacatcctg
     1081 acggtcaaca tcgacatcga cgaccgcgcc cagacccaga actcgaccaa ccggtcgcgc
     1141 accaacgaca tctcgggcgg gatctcgaac ttcttcggac tggagaacag cctcggccgc
     1201 gccttccccg gcggcttcga cccccagaat ctggtcgggc tggaaggcga gtccaacgcc
     1261 accggatccg ggtcggtcaa tcggtccgaa aaggtcagcc tgaccatcgc cgccgtcgtc
     1321 accggcgtca tggccaacgg caatctggtg atccagggcc ggcaggaggt gcgcacgaac
     1381 cgcgaagttc gcgaactgac tgtggccggc atcgtgcggc ccgaggacat cagctcgggc
     1441 aacaccatcg cccacaccca gatcgccgag gcccgcatca gctacggcgg tcgcggcgac
     1501 atcagccggg ttcaggccac gcccgcggcc caggcgctgg ttgagcgctt cagcccgttc
     1561 tgattgggcc gcggtcgtcg gcccctcgag acctcccgac ccgagcggcc tggccatccg
     1621 cacgcgccat tttcgccaca aggcgaaaac tgccgggcgc cggccaagcg gagccgtgaa
     1681 ccgttaatga agcacagcgt ttggccatca gacgcgagtg ttcccatgct gaagttcgcc
     1741 atccccgccg tcgccgccct cgggctgatc gcgtttgccg cccaatcaca ggcgacgaat
     1801 tcggacgccc ggacgggcgc tgcggcgccc atggcgtggc acctcagtca tgagggcgat
     1861 ctggccaaac tggcctatgg cgtcgcgaac tcggaccagc tggcgctgat gctgacctgc
     1921 gaaccggctc aggcccaggc ggtggtttac ggcgacgtca aaccggccgg gccccgtgtc
     1981 cagaaggcct cgatgcgcgc cgtcgccatc gatcccctga gcggcgga
//
LOCUS       DASVVN010000275         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_141981,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000275 DASVVN010000000
VERSION     DASVVN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141981"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /gene="lpdA"
                     /locus_tag="VF686_04350"
     CDS             complement(<1..1185)
                     /gene="lpdA"
                     /locus_tag="VF686_04350"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8661075.1"
                     /translation="MADSPSTYDVVIIGGGPGGYNAAIRAGQLGLKAACVEMRETLGG
                     TCLNVGCMPSKALLHASELFEAATKEFPTLGIEIGTPKLNLGQMMGQKADSVGALTKG
                     IEFLFKKNKVEWIRGRGRIVAKGQVEVTAADGSKSTLSAKSIVIATGSEPTPLPGVAF
                     EDGKVVDSTGALSLPAVPKHLIVVGAGIIGLELGSVWRRLGAQVTVVEFLDRITPGMD
                     KDVATAFQRALTKQGMTFRLGAKVTAAKTGKDGVELTVEPAAGGTAETLKGDVVLVAI
                     GRRPYTEGLGLETVGVETDKRGFVAGDHFRVADGVWVIGDVTHGPMLAHKAEEDAVAV
                     IELIAGKAGHVDYDLVPSVVYTAPEVAWVGKTEDQLKAEGVAYKAGKFPFTANSRAKI
                     NHE"
     gene            complement(1313..1795)
                     /locus_tag="VF686_04355"
     CDS             complement(1313..1795)
                     /locus_tag="VF686_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661076.1"
                     /translation="MKILRLIVMLVVLGYAGWLAWPFVSPLIEGAAPEAAARAGTAAS
                     SGAELFGFLPMWALWAGAVGLYVIAALMLGAGNAKASIAYFLAFAADVVLRLAIDRQG
                     DVSARSTGPTTMAAPEAIQGLPVEPVWIILGALFLLGVLVIVASRRIRRKRTAGQLNF
                     "
     gene            complement(1860..3665)
                     /locus_tag="VF686_04360"
     CDS             complement(1860..3665)
                     /locus_tag="VF686_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8661077.1"
                     /translation="MFLEGQINWMWVWAAAAAAGWLAALVAIFFALRRGAQHGSATDQ
                     LDLIQRVAEDSQKRLFETLNAIPVALVETDKQGKFVFANRAAHQLLGRKDAELIGLRF
                     HSATWGITFPDGRPVPPDLLPSARALRGQTVRGFQHLLANPAARRKMLVSVTAMPIEN
                     ELGQITGSTAAIVESEGLQTPEIIMPEPVAPAASPASHDGLTRRVFDAASSALLVIDP
                     DGQVREANHTALLLLGRDSAAGDFADQFLAEDERVEGRQTLRAALSAPAGEADPIVSR
                     NGGAGGVRWSILPLTAVDGTVDALLLAGERADAATPVEIAEPTPEGESPADPSPEIDA
                     LREAANAARRQADEALAAAAAAREEAQAARAAAVKAEQDAEARLQGGRRMENVGRLTG
                     GLAHDFNALLGVMTGALDMMLRQADDPLRVRRIGQAALTAGQKGELLTRRLTAFSQGD
                     DEPVLRVLDAGVLLRGMESRLRAMAGPGVDLMIEGPSVDAPVRLDPVAFEGAVSGLTR
                     NAVDAVDGQGSVAVRLEVMLEGGVRLSVRDSGPGMDRDLAARAVEPFFTTREGAAGLG
                     LSQAYAFSRQSGGVLSIDSAPGEGAEVSITLPNAG"
     gene            complement(3766..>4236)
                     /locus_tag="VF686_04365"
     CDS             complement(3766..>4236)
                     /locus_tag="VF686_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661078.1"
                     /translation="ALGPRRDGRATMLSLAIVFSVLVFVFPLRLLIETAAHFVSGGWL
                     PGAGLISNFGELGLTYLIYGLGFSVLSLLFALLFRQAEATIDPDDAVRRRAAFEWTRT
                     WALAAATGVVSGLIALTPALRAAPWLPGVTYWLIPAGIGVFAVIDRRRARTAAD"
BASE COUNT          655 a         1514 c         1462 g          605 t
ORIGIN      
        1 ctcgtggttg atcttggcgc ggctgttggc ggtgaagggg aatttcccgg ccttgtaggc
       61 gacgccctcg gccttcagct ggtcctccgt cttgccgacc caggccactt ccggcgcggt
      121 gtagaccacg ctggggacca gatcgtagtc gacgtggccc gccttgcctg cgatcagctc
      181 gattacggcc acggcgtctt cctcggcctt gtgcgccagc atcgggccgt gggtcacgtc
      241 gccgatgacc cagacgccat ccgccaccct gaagtgatcg ccggccacga agccgcgctt
      301 gtcggtctcg accccgacgg tctccagccc cagaccctcg gtgtagggac gacggccgat
      361 ggccaccagg accacgtcgc ccttcagcgt ctcggcggtt cctcccgcgg ccggctcgac
      421 ggtcagttcg acgccgtcct tgccggtctt cgcggccgtg accttggcgc ccagcctgaa
      481 ggtcatgccc tgtttggtca gcgcccgctg gaaggccgtc gccacgtcct tgtccatgcc
      541 gggggtgatg cggtcgagga attcgaccac cgtcacctgc gcccccaaac ggcgccagac
      601 cgagcccagc tccagaccga tgatgccggc gccgacgacg atcagatgtt tcggcaccgc
      661 gggcagcgac agggcgccgg tcgagtcgac gaccttgccg tcctcgaagg ccacgcccgg
      721 cagcggcgtc ggctcggagc cggtggcgat gacgatgctt ttggcgctca aggtcgactt
      781 cgacccgtcc gccgcggtca cctcgacctg acccttggcg acgatgcggc cgcggcccct
      841 gatccactcg accttgttct tcttgaacag gaattcgatg cccttggtca gggcaccgac
      901 gctgtcggcc ttctggccca tcatctggcc gaggttcagc ttcggcgtgc cgatctcgat
      961 gccgagcgtc ggaaactcct tcgtcgccgc ctcgaacagc tccgaggcgt gcagcaaggc
     1021 tttggagggc atgcagccga cattcaggca ggtcccgcca agcgtctcgc gcatctctac
     1081 gcaggcggcc ttgaggccca gctggccagc gcggatcgcc gcattatacc cgcccggacc
     1141 gccgccgatg atgacgacgt cataggtgct gggggaatcg gccatggggt ctctcgtctg
     1201 tgtgggcgcg gggaggcgcg cggaacctga cggcgccgct cgcgagcgtc aaccgcgccg
     1261 ccgcatatag gcgcggcgaa ccgcgtccga cagacgccgg aggtcagcga cgtcagaagt
     1321 tgagctggcc cgccgtccgc ttgcgccgga tgcgccggct ggcgacgatc accagcacgc
     1381 cgagcaggaa gagggcgccc agaatgatcc ataccggctc gaccggcagc ccctggatcg
     1441 cctcgggcgc cgccatggtc gtcgggcccg tggaacgggc gctgacgtcg ccctgacggt
     1501 cgatggccag acgcaggacc acgtccgcgg cgaaggcgag gaaataggct atcgacgcct
     1561 tcgcattccc cgcccccagc atcagggcgg cgatcacata caggccgacg gcgccggccc
     1621 acagggccca catcggcagg aagccgaaca gttcggcgcc ggaagacgcg gccgttcctg
     1681 cgcgcgccgc ggcctcgggc gccgcgccct cgatcaacgg agagacgaac ggccaggcca
     1741 gccatccggc atagccgagc accaccagca taacgatcag acgcaggatc ttcatttcga
     1801 gcctcccaac tcccgttaac cttataacgg tctgccgaaa gcgcaaaagg aggcgggcgt
     1861 cagccggcgt tgggcagggt gatcgagacc tcggcgccct cgcccggggc gctgtcgatc
     1921 gacaggacgc cacccgactg gcgcgagaag gcataggcct gcgacaggcc cagccccgcc
     1981 gccccctcgc gggtggtgaa gaagggttcg accgcgcggg cggccagatc ccgatccatg
     2041 cccggcccgc tgtcgcggac gctgagacgc accccgccct ccagcatgac ctccagccgc
     2101 acggcgaccg agccctgacc gtcgacggcg tcgacggcgt tgcgcgtcag tccgctgacc
     2161 gcgccctcga aggcgaccgg gtccagccgc accggagcgt cgacggacgg tccttcgatc
     2221 atcaggtcca cgcccggccc ggccatggcg cgcaaccggc tctccatgcc gcgcagcagc
     2281 acgccggcgt ccagcacccg cagaacgggc tcgtcgtcgc cttgcgagaa ggcggtcagg
     2341 cggcgggtca gcagttcgcc cttctggccg gcggtcagcg cggcctggcc gatgcgccgc
     2401 acgcggaggg ggtcgtcggc ctggcgcagc atcatgtcca gagcgccggt catgacgccc
     2461 agcagggcgt tgaaatcgtg cgccagaccg ccggtcaggc gcccgacatt ctccatccgg
     2521 cgcccgccct gaagccgggc ctcggcgtcc tgttcggcct tcacggcggc ggcgcgtgcg
     2581 gcctgcgcct cctcccgggc ggcggcggcg gcggcgaggg cctcgtcggc ctgacggcgg
     2641 gcggcgttcg ccgcctcgcg cagggcgtcg atctcgggcg agggatcagc aggggactcg
     2701 ccttcgggag tcggctcggc gatttcgacc ggcgtagcgg cgtccgcgcg ctcgccggcc
     2761 agcagcaggg cgtcgacggt cccgtcgact gcggtcaggg gcaggatgga ccagcgcacg
     2821 cccccggcgc cgccgttccg ggacacgatg ggatcggcct cgcccgccgg cgccgacagg
     2881 gcggctcgca gcgtctgccg gccctcgacc cgctcgtcct cggccaggaa ctggtcggcg
     2941 aagtcgccgg cggcgctgtc gcggcccagc agcagcaggg cggtgtggtt ggcctcgcgc
     3001 acctgcccgt ccggatcgat gacgagcaag gcgctggagg cggcgtcgaa cacgcgccgg
     3061 gtcaggccgt catgagaggc gggcgaagcg gcgggcgcga ccggctcggg catgatgatt
     3121 tcgggcgtct gcagaccttc gctctcgaca atggcggcgg tcgagccggt gatctggccc
     3181 agttcgttct cgatcggcat ggccgtgacc gagaccagca tcttgcggcg cgcggcgggg
     3241 ttggccagca gatgctggaa cccccgcacc gtctggccgc gcagcgcgcg ggcgctcggc
     3301 agcaggtcgg gcggcacggg acggccgtcc gggaaggtga tgccccaggt cgccgaatgg
     3361 aagcgcaggc cgatcagctc ggcgtccttg cggcccagca gctggtgggc ggcgcggttg
     3421 gcgaagacga atttgccctg tttatcggtc tcgaccagag ccacgggaat agcgttcagc
     3481 gtctcgaaca gccgcttctg cgaatcctcg gcgacccgct gtatcaaatc gagctggtcg
     3541 gtggcgctgc cgtgctgcgc gcctctacgc agggcgaaga agatcgcgac cagggccgca
     3601 agccagcccg ccgcagcggc ggcggcccaa acccacatcc agttgatctg gccttcgaga
     3661 aacatgcgac ccgactgtcc cgttctggcg cctctgcgcc tgttcgggag ataccttgca
     3721 aatggcgaac cggaccagcc cggtttcgcc cttgaggcga cctgttcagt cggccgcagt
     3781 ccgggcgcgg cgccggtcga tcacggcgaa tacgccgatg ccggccggga tgagccagta
     3841 ggtcacgccc ggaagccagg gcgcggccct cagagccggc gtgagcgcga tcaggcccga
     3901 gacgactccg gtcgccgcgg caagcgccca ggtgcgcgtc cattcgaacg ccgcgcgccg
     3961 tcggaccgcg tcatccgggt cgatggtggc ttcggcctgc cggaacagca gggcgaacag
     4021 caacgacagg accgagaagc ccaggccgta gatcagatag gtcagtccca gctcgccgaa
     4081 attactgatc agccccgccc ccggcagcca tccgcccgaa acgaaatgcg ccgccgtctc
     4141 gatcagcagc cgcaggggga agacgaaaac cagcaccgag aagacgatgg cgaggctgag
     4201 catcgtggcc cgtccatccc gccgcggccc cagcgc
//
LOCUS       DASVVN010000276         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_142150,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000276 DASVVN010000000
VERSION     DASVVN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142150"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VF686_04370"
     CDS             complement(<1..642)
                     /locus_tag="VF686_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661079.1"
                     /translation="MTDAYISDPIRHPLHTLISWPAVIAGGIVAVAVGAMLNLLGVAL
                     GASAFNPYEFGGGDAEGFTAAAGMWMAFANAIALFIGGAVATRAAKYADHHKGALHGL
                     AVWALSFLLAITIASAAGAGGAAALVGGEAADSDNRNVVSDIAPASRVIEGELVAPVV
                     VQPSAPAAAEEVADTTAMLALWGFLTMLIGAIAAIFGGLYGGRNHRWIDKAVDG"
     gene            complement(717..1253)
                     /locus_tag="VF686_04375"
     CDS             complement(717..1253)
                     /locus_tag="VF686_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX8661080.1"
                     /translation="MTHRIILFRGMNTGGVRAPVGEQRAMAEAMGLKNPRTLAASGNL
                     VVESGKATDALERDIEAAMEKTFGLKVAAMVRTPDQWAKLIAANPFSKEAAAHPAKVI
                     AMVMKDGIRDGALEACRALAEGGEAVEAVDGVLYFWFPHGQGESGIFKKATPRMLGMG
                     TGRNWNTVLKLADMAGAA"
     gene            complement(1250..1804)
                     /locus_tag="VF686_04380"
     CDS             complement(1250..1804)
                     /locus_tag="VF686_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX8661081.1"
                     /translation="MPIHIALMRSVVINGRRVKGEEARSLAEAAGAAGIRSVGATGNL
                     LFRSRKAPATLERDLEAACAALYGQPTEMIVKTADQWRALMKANPFAERSETSPARIL
                     VWAMRDPLPDSGLDQLRRRAAADEAVERTTSGDIYMWFGGHDPNASKLPAGFGLKSLG
                     AVGTNRNWNTARKITAALDEMESA"
     gene            complement(1818..2681)
                     /gene="mutM"
                     /locus_tag="VF686_04385"
     CDS             complement(1818..2681)
                     /gene="mutM"
                     /locus_tag="VF686_04385"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX8661082.1"
                     /translation="MPELPEVETVRRGLEPVLTGARLTRVRQNRPDLRFPFPDRFVDR
                     LEGATVERIDRRAKYLLMPLSTGETWVTHLGMTGRFTLDGEQLGEFEEAAPVAGKHEH
                     VSQSAVKGKATTRLGFADARRFGFMGLIPTGEVDAHPWFAGLGPEPLGNGFSGAHLAE
                     AFAGKKQNIKVSLLDQRIVAGLGNIYVCEALYRARISPLTAAGKLTKPRLETLAAVVR
                     DVLDDAIAAGGSTLKDFANADGGQGYFQHRFDVYGREGKPCLGDGCTGVVKRLVQAGR
                     STFWCPSCQKR"
     gene            complement(2681..>2926)
                     /locus_tag="VF686_04390"
     CDS             complement(2681..>2926)
                     /locus_tag="VF686_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661083.1"
                     /translation="AYLAGRIGRDEAQTLLGKYTLAEPARAAQRLRFIDTYRSYIINY
                     GLGRDVVQAWVERQGPNRWDGMETLLSSQILPVDLLP"
BASE COUNT          411 a          968 c         1082 g          465 t
ORIGIN      
        1 gccgtcgacc gccttgtcga tccagcggtg attgcggccg ccgtacagac cgccgaagat
       61 cgcggcgatg gcgccgatca gcatggtcag gaagccccac agcgccagca tggccgtggt
      121 gtcggcgact tcctcggcgg cggccggggc ggaaggctgg accaccaccg gcgcgaccaa
      181 ttcgccctcg atcacccggc tggcgggcgc gatgtcgctc accacattgc ggttatcgct
      241 gtcggcggcc tcgcctccaa ccagcgccgc cgcgccgccg gcgcccgcgg cgctggcgat
      301 ggtgatggcc agcaggaagg acagggccca gacggcgagg ccgtgcaggg cgcccttgtg
      361 atggtcggcg tatttggcgg cccgggtggc gacggcgccg ccgatgaaca gggcgatggc
      421 gttggcgaag gccatccaca tgccggcggc ggcggtgaag ccctcggcgt cgccgccgcc
      481 gaactcatag gggttgaagg ccgaggcgcc gagcgcgacg cccagcaggt tcagcatggc
      541 gccgaccgcg acggccacga tcccgccggc gatgacggcc ggccacgaga tcagggtgtg
      601 aagcgggtgg cggatggggt cggaaatata ggcgtcggtc atggctctcc ctccagggtg
      661 tttaccttgg ggccaaaacc gctgtgcagc gctgcggttc cggatgctgt ggcacgtcag
      721 gccgcgccgg ccatgtccgc cagctttagg accgtgttcc agttgcggcc ggtgcccatg
      781 cccagcatgc gcggcgtggc cttcttgaag atgccggact cgccctggcc gtgggggaac
      841 cagaaataga gcacgccgtc gacggcctcg accgcctcgc cgccttccgc cagcgcacgg
      901 caggcctcga gcgcgccgtc cctgatcccg tccttcatca ccatggcgat gaccttggcg
      961 gggtgggcgg cggcctcctt ggagaagggg ttggcggcga tcagtttcgc ccactggtcg
     1021 ggggtgcgga ccatggcggc gaccttgagg ccgaaggtct tttccatggc ggcttcgatg
     1081 tcgcgctcca gcgcatccgt ggccttgccg ctctcgacca ccagattgcc gctggccgcg
     1141 agggtgcggg ggttcttcag tcccatggcc tcggccatcg cccgctgctc gccgacgggc
     1201 gcgcggacgc cgccggtgtt catgccgcgg aacagaatga tgcggtgggt catgcggatt
     1261 ccatctcgtc gagggcggcg gtgatcttgc gcgccgtgtt ccagttgcgg ttggtgccga
     1321 cggcgccgag cgatttcagg ccgaagccgg cggggagctt cgaggcgttc gggtcatggc
     1381 cgccgaacca catatagatg tcgccggatg tcgtgcgctc gaccgcctcg tcggcggcgg
     1441 cgcgacggcg cagctggtcg aggccgctgt cgggcagggg atcgcgcatc gcccagacga
     1501 ggatgcgggc gggcgaggtc tccgaccgct ccgcgaaggg gttggccttc atcagggcgc
     1561 gccactggtc ggcggtcttg acgatcatct cggtgggctg gccgtacagg gcggcgcagg
     1621 ccgcctcgag atcgcgctcg agcgtggccg gcgccttgcg cgagcggaac agaagattgc
     1681 cggtggcgcc gaccgagcgg atcccggccg cgcccgccgc ctcggcgagg cttcgcgcct
     1741 cctcgccctt cacgcgacgg ccgttgatga cgacgctgcg catgagggcg atatggatcg
     1801 gcaaggcggc gggctcctca gcgcttctgg caggaggggc accagaaggt ggaccggcca
     1861 gcctgaacca gacgtttgac cacgccggtg cagccgtccc ccaggcaagg ttttccctcg
     1921 cggccgtaga cgtcgaagcg gtgctggaaa tagccctgtc cgccatcggc gttggcgaaa
     1981 tccttcagcg tcgagccgcc ggcggcgatg gcgtcgtcga gcacgtcgcg gactacggcg
     2041 gcgagggttt cgagacgcgg cttcgtgagt ttgcccgccg ccgtcagggg cgagatgcgt
     2101 gcgcggtaca gggcctcgca gacatagata ttgcccagac ccgcgacgat gcgctggtcg
     2161 agcaggctga ccttgatgtt ctgcttcttg ccggcgaagg cctcggccag atgggcgccg
     2221 gagaagccgt tgccgagcgg ttcggggccg aggccggcga accatgggtg ggcgtccacc
     2281 tcgccggtcg ggatcaggcc catgaagccg aagcggcggg cgtcggcgaa gccaaggcgg
     2341 gtcgtcgcct tccctttcac cgcgctctgg ctgacgtgct cgtgcttgcc ggcgacgggg
     2401 gccgcctcct cgaattcgcc cagctgttcg ccgtccaggg tgaagcggcc ggtcatgccg
     2461 aggtgggtga cccaggtctc gccggtcgac agcggcatca gcagatattt ggcgcggcgg
     2521 tcgatgcgct cgaccgtcgc cccctccagt cggtcgacga accggtcggg aaaggggaag
     2581 cgcaggtcgg gccggttctg gcgcacgcgc gtcagtcgcg ccccggtcag caccggttca
     2641 aggccgcggc ggacggtttc gacctcggga agctccggca tcagggcagc aggtcaaccg
     2701 gcaggatctg gctcgagaga agggtctcca tgccgtccca gcggttcggc ccctgccgct
     2761 cgacccaggc ctggaccacg tcgcggccga ggccgtagtt gatgatgtag ctgcggtagg
     2821 tgtcgatgaa gcgcagccgc tgggccgccc gcgccggttc ggcgagcgtg tatttcccga
     2881 gcagcgtctg cgcttcgtcg cgcccgatcc gccccgccag ataggc
//
LOCUS       DASVVN010000277         5608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_404897,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000277 DASVVN010000000
VERSION     DASVVN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404897"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1265
                     /locus_tag="VF686_04395"
     CDS             75..1265
                     /locus_tag="VF686_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8661084.1"
                     /translation="MADGSSAPQKTFRWEDPLDLSGRLTDDERMIQDAARAYAREKLL
                     PRVVEAFRDETFDRAIMTELGEMGFLGAMLPEQYGGSSVSHVAYGLIAREIEAVDSGY
                     RSAMSVQSSLAMYPIYAFGSEEQKMRFLPRMAAGELVGCFGLTEADGGSDPASMKTKA
                     VAVDGGYKLNGAKYWITNSPISDLAVVWAKLDDTIRGFIVERGMDGFTTDKIGDKLSL
                     RASITGDIGLNDVFVPEENLLPGVKGLRGPFSCLNKARYGIAWGAMGAAEACFHLTRD
                     YVGERMLFGRPLSSRQLVQKKLADMQTEIFLGFEGALALGRLLDQGAWVPEAISMMKR
                     NNCGKALAIAREARDMHGGAGITGEMHVMRHAMNLETVNTYEGAHDVHALILGRAITG
                     ESAF"
     gene            1328..2656
                     /locus_tag="VF686_04400"
     CDS             1328..2656
                     /locus_tag="VF686_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8661085.1"
                     /translation="MRRFNLRAGGLSMLALVAAVPLLTASPAAAQQSPVPFDAERGVF
                     TFATGLERALPAVVQVTTLGQSEGPSSGENEPKPVSSGSGAIIDAREGIVVTNNHVVE
                     GGRKFTVDMTDGRIFDATLIGTDKATDLAVLKIEPGDRTLGLSQIEVVNSDSLRTGDL
                     AFAVGYPLGLDQTLTMGVISGLGRSGMGDRIEDYIQTDAAVNSGNSGGPLLDSRGRLI
                     GINTSILSGGGGGNDGIAFAVPSRILMYVVAQLRESGEVKRGTTGVILGSLNAERSRD
                     FGLGIVRGAVVEDIAPGSSAERGGLRRGDVITRIQNRPVSNAGTVGATVGMAAPGTQV
                     EVVYLRDGREGRTTITVEAPSDRPVISGADAVIARGAAVRLAGDGLQVFSVEGGSVAA
                     AADLQAGDMIRSVDGQAIADLAALAAAIDGSGERILSVTRGEEAVEIRLP"
     gene            2994..3326
                     /locus_tag="VF686_04405"
     CDS             2994..3326
                     /locus_tag="VF686_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX8661086.1"
                     /translation="MSDAADLQSQPGPVFFDRKELDLILRVYGRMVAAAEWRDYAMVG
                     HKDVAEFAVFRRSGDAPLYRIEKRPALRLKQGQWAVIGEGGHVLRRGRELAQVLRVFD
                     SRKFTIVD"
     gene            complement(3382..4137)
                     /locus_tag="VF686_04410"
     CDS             complement(3382..4137)
                     /locus_tag="VF686_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX8661087.1"
                     /translation="MSDFRNGYTAPATADMSVDAGLRSFMLGVYNKVALGLLVSAVLA
                     YITGSYPPVRDLMYATAVFPDGLTRLTGLTVLGMIVAFSPLVILLGSNFVMKNPTSGG
                     ASALYWVIVALIGASMGTVVLMYTGASVVQTFLITAAAFGALSLFGYTTKKDLTGFGS
                     FLIMGVIGLIIASVVNIFLQNSMMQLIISVLGVLIFAGLTAYDTQRLKMTYYALGGNQ
                     NAMGVATSYGALSLYINFINMFQFLLALFGGRR"
     gene            4262..4801
                     /gene="thpR"
                     /locus_tag="VF686_04415"
     CDS             4262..4801
                     /gene="thpR"
                     /locus_tag="VF686_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8661088.1"
                     /translation="MIRLFTALSVPDDVTETLARRQTGLPGAKWRTAEQLHITLTFYG
                     AVDERRADDLAIELERAAMGGPFEIALQGVGAFGDGHRTQTLWAGIEPNERLSVLAGR
                     TRSAAERAEIAVERRAYHPHLTLAYLKPHANPDRIGAWIAGHNLLHSPPIRIDRFGLY
                     SSVLTHDGSHYELEREYLL"
     gene            4798..5337
                     /locus_tag="VF686_04420"
     CDS             4798..5337
                     /locus_tag="VF686_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8661089.1"
                     /translation="MTGPVLETERLTLRPIAMEDFPRWAEMMGDPEAAKFLGGAQPAA
                     TTWRGFMTMAGAWSLTGVSMFSLIERASGLWLGRIGPWQPLGWPGTEVGWGLHPDAQG
                     KGYGVEAATATIDYAFDVLGWTEVIHCIDPDNIPSQRLAERVGSRNLGPTRLPPPFHE
                     LTIDRWGQSREEWRARDRG"
BASE COUNT          944 a         1870 c         1902 g          892 t
ORIGIN      
        1 gggccgtccc tctacccgac cggtgcattt tggcctaaac cgcgcgcgac cgccacgctc
       61 aggaaagacc atcgatggcc gacggcagct ctgctcccca gaagaccttc cgctgggaag
      121 acccgctgga cctgtcaggc cggctgaccg acgacgagcg gatgatccag gacgcggccc
      181 gcgcctacgc ccgcgagaag ttgctgcccc gcgtggtcga ggctttccgc gacgagacct
      241 tcgaccgcgc catcatgacc gagcttggcg agatgggctt cctcggcgcc atgctgcccg
      301 aacagtacgg cggctcctcg gtcagccacg tcgcctatgg cctgatcgcc cgcgagatcg
      361 aggcggtaga cagcggctac cgctcggcca tgtcggtcca gtcgtcgctg gcgatgtacc
      421 cgatctacgc cttcggctcg gaagagcaga agatgcgctt cctgccccgc atggccgccg
      481 gcgaactggt cggctgtttc ggcctgaccg aggccgacgg cggctcggac cccgcctcga
      541 tgaagaccaa ggccgtggcg gtcgacggag gctacaaact caacggcgcc aaatactgga
      601 tcaccaactc gccgatcagc gatctggcgg tggtctgggc aaagctcgac gacaccatcc
      661 gcggcttcat cgtcgaacgc ggcatggacg gcttcaccac cgacaagatc ggcgacaagc
      721 tgagcctgcg cgcttcgatc accggcgaca tcggcctgaa cgacgtcttt gtgccggaag
      781 agaacctgct gcccggcgtg aaaggcctgc gcggcccctt ctcatgcctg aacaaggccc
      841 gctatggcat cgcctggggg gccatgggcg ccgccgaggc ctgtttccac cttacccgcg
      901 attatgtcgg ggaacgcatg ctgttcggcc gccccctgtc ctcgcgccag ctggtgcaga
      961 agaagctggc cgacatgcag accgagatct tcctcggctt cgagggcgcg ctggcgctgg
     1021 gccggctgct ggatcagggc gcctgggtcc ccgaagccat ctcgatgatg aagcgcaaca
     1081 actgcggcaa ggccctggcc atcgcccgcg aggcccgcga catgcatggc ggcgccggca
     1141 tcaccggcga aatgcatgtc atgcgccacg ccatgaacct cgagacggtc aacacctacg
     1201 agggcgccca tgacgtccac gcgctgatcc tcggccgggc catcacgggc gagagcgcct
     1261 tctaggtttg gcttcgacaa cccgtcgtgt cccgcgctag ggtcgggcga tgacggggaa
     1321 ttcagcgatg cggcgtttca atcttcgggc cggcggactg tccatgctgg cgctcgtcgc
     1381 cgcggttccg ctgctgacgg cctcgccggc cgcggcccag cagtcgccgg tgccgttcga
     1441 cgccgagcgc ggcgtcttca ccttcgccac cggcctcgag cgcgccctgc ccgcggtggt
     1501 ccaggtcacg accctgggcc agtcggaagg cccgagctcg ggcgagaacg agcccaagcc
     1561 ggtgtccagc ggatccgggg ccatcatcga tgcgcgcgaa ggcatcgtcg tcaccaataa
     1621 tcacgtggtc gagggcgggc ggaaattcac cgtcgacatg actgacggcc gcatcttcga
     1681 cgccaccctg atcggcaccg acaaggccac ggacctcgcc gtgctgaaga tcgagcccgg
     1741 agacaggacg ctgggcctgt cccagatcga ggtggtgaat tcggattcgc tgcgcaccgg
     1801 cgatctggcc ttcgcggtcg gctatccgct gggtctggac cagacgctga ccatgggcgt
     1861 gatctcgggt ctcggccgtt cggggatggg cgaccggatc gaggactata tccagaccga
     1921 cgcggccgtg aactcgggta actcgggcgg accgctgctg gacagccggg gccggctgat
     1981 cggcatcaac acctccatcc tgtcgggcgg cggcggcggc aacgacggca tcgccttcgc
     2041 cgtgccaagc cgcatcctga tgtatgtcgt ggcccagctg cgcgagagcg gagaggtcaa
     2101 acggggaacc accggcgtca ttctcggctc actgaacgcc gagcggtcgc gcgacttcgg
     2161 tcttggcatc gtgcgcggcg ccgtcgtcga ggacatcgcc cccggctcgt cggccgagcg
     2221 cggcggcctg cgccggggcg acgtcatcac ccgcatccag aaccggcccg tgtcgaacgc
     2281 gggcacggtc ggcgcgacgg tcggcatggc cgcgccgggg acccaggtcg aggtcgtcta
     2341 tctgcgcgat ggccgcgaag gccgcaccac catcaccgtc gaggccccga gcgaccgccc
     2401 ggtcatctcc ggcgccgacg ctgtaatcgc ccgcggggcg gccgttcgtc tggccggcga
     2461 cgggctgcag gtgttttcgg tggaaggcgg ctccgtcgcg gcggcggccg atctgcaggc
     2521 cggcgacatg atccgctcgg tcgacggtca ggcgatcgcg gacctggccg ccttggccgc
     2581 tgcgatcgac ggaagcggag agcgtatcct gtcggtgaca cggggcgagg aagcggtcga
     2641 gatccgcctg ccctgaccta cccgacgccg gacagcaaaa cgcccgctct gccggagggg
     2701 ggagggtggc ggagcgggcg tctcgctttg gaggaagcat cggcccgccg gagcccttgg
     2761 tggagaaatc caggaggggc tgggggggct gttcaggatc cggaactgct ccgaactcca
     2821 acgagccgat gacccagact tcaccttgca aggcggcggg cgatgggcgg aagcgaggcg
     2881 atttcatgtt cggtgcgatt tcctccggag gctgttcttg ccgacacagt gttcgctgaa
     2941 gatcgggcga cgggagtctg gcgtttcgcg agggtcggct taccttgatg ctgatgagcg
     3001 acgcggcgga tttgcagtca cagcccggcc cggtcttctt cgaccgcaag gagctggatc
     3061 tgattctgag ggtctacggc cggatggtcg cggccgccga gtggcgcgac tacgccatgg
     3121 tggggcacaa ggacgtggcc gagttcgccg tcttccgccg ctcgggcgac gccccgcttt
     3181 accggatcga gaagcgtccg gccctgcgcc tgaaacaggg ccagtgggcg gtcatcggag
     3241 aaggcggcca tgtgctgcgc cgggggcgcg aactggccca ggtgctgcgg gtcttcgaca
     3301 gccgcaaatt cacgatcgtg gattagaaaa aagccccggc gcttccgccg gggctttcca
     3361 tgtcgtgctg tcagcgaggc gtcagcgacg gccgccgaac agcgccagca ggaactggaa
     3421 catgttgatg aagttgatgt acaggctcag cgccccgtag ctggtggcca cgcccatcgc
     3481 gttctggtta ccgcccaggg cgtagtaggt catcttcaga cgctgggtgt cgtaggcggt
     3541 caggcccgcg aagatcagca cgcccagaac gctgatgatc agctgcatca tggagttctg
     3601 gaggaagatg ttgaccaccg aggcgatgat caggccgatc acgcccatga tcaggaagct
     3661 gccgaagccg gtcagatcct tcttggtcgt atagccgaac aggctcagcg cgccgaaggc
     3721 ggcggccgtg atcaggaagg tctggaccac cgaggcgccg gtgtacatca gcaccacggt
     3781 acccatcgaa gcgccgatca gcgcgacgat cacccagtac agcgcgctgg cgccgcccga
     3841 ggtcgggttc ttcatcacga agttggagcc cagcaggatg accagcggcg agaaggccac
     3901 gatcatgccc agcacggtca ggcccgtcag ccgggtcagg ccgtcgggga agaccgcggt
     3961 cgcgtacatc aggtcgcgga ccggcgggta ggacccggtg atgtaagcca gcacggccga
     4021 cacgagcagg cccagggcca ccttgttgta aacgcccagc ataaagctgc gcaggccggc
     4081 gtcgaccgac atgtcagctg tcgcgggcgc cgtatagccg tttctgaaat cgctcatggc
     4141 gagatcgatc ctccatctga ccgggcgatc ccggcctttg cacaaatatc gggccggtcc
     4201 gccccttccg caaggaaaac cggacttggg gcctgcgttc ccaaccgcta ccaagggtcg
     4261 catgatccgg ctgttcaccg ccctgtccgt tccagacgac gtgaccgaga cgctggcgcg
     4321 ccgccagacc ggcctgcccg gcgcgaaatg gcgcacggcc gaacagttgc acatcaccct
     4381 gaccttctat ggcgcggtgg acgaacgtcg tgccgatgat ctggccatcg agctggagcg
     4441 ggcggcgatg ggcggtccct tcgagatcgc gcttcagggc gtcggcgcct tcggagacgg
     4501 ccatcgcacc cagaccctgt gggccgggat cgagccgaac gaacgactgt cggtgctggc
     4561 cgggcgcact cgcagcgccg ccgaacgggc tgaaatcgcc gtcgagcgcc gcgcgtacca
     4621 cccgcatctg acgctggcct atctgaagcc gcacgccaat ccggaccgga tcggcgcctg
     4681 gatcgcgggc cataatctgc tgcattcgcc cccgatccgg atcgaccggt tcggcctcta
     4741 ctccagcgtc ctgacccacg atggcagcca ttacgaactc gaacgggagt atctgctttg
     4801 accggaccgg tgctggaaac cgaacgcctg accctgcggc ccatcgcgat ggaggatttt
     4861 ccgcgctggg ccgagatgat gggcgatccc gaggcggcga aattcctcgg aggcgcccaa
     4921 ccggccgcaa ccacgtggcg cggtttcatg accatggccg gcgcgtggag cctgaccggg
     4981 gtctccatgt tctcgctgat cgagcgggcc agcggcctct ggctcggccg catcggccct
     5041 tggcagccgc tcggctggcc ggggaccgag gtcggctggg gtctgcaccc ggacgcccag
     5101 ggcaagggct atggcgtcga ggccgcgacg gcgacgatcg actatgcctt cgacgtgctg
     5161 ggctggacag aggtcatcca ctgcatcgat ccggacaaca tcccgtccca gcggctggcc
     5221 gagcgggtcg ggtctcgcaa tctgggtccc acccgcctgc cccctccctt ccatgagctg
     5281 accatcgacc gctggggtca gagtcgcgaa gagtggcgag cccgcgaccg gggctgaacc
     5341 cgccacgccg cttcacgcgt tacaaacgtc aagctttgac cccacgccgc catccggtag
     5401 gcagggtccg ggcaatcacg agggatggtc gaatgcgggt tcttgttctg ggcggcgacg
     5461 gtttctgcgg ctggccgaca gccttgcacc tgtcggcgcg cggctgggac gtctgcgtcg
     5521 tcgacaactt gagccggcgc aatatcgaca acgagctgga ggtccagtcc ctgaccccga
     5581 tccggacgat gggcgaacgc atcgccgc
//
LOCUS       DASVVN010000278         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_62611, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000278 DASVVN010000000
VERSION     DASVVN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62611"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF686_04425"
     CDS             complement(<1..215)
                     /locus_tag="VF686_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX8661090.1"
                     /translation="MVKKFAAEFVGTLVLVLFGCGAAVLGGFDHVGQLGIALAFGFAI
                     IAMAYGIGPVSGCHVNPAVSLGAFVAGR"
     gene            complement(288..602)
                     /locus_tag="VF686_04430"
     CDS             complement(288..602)
                     /locus_tag="VF686_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HEX8661091.1"
                     /translation="MHLKTLQADVLRISDIYAAEHGIERSADWALLKIQEELGELTAE
                     HLRMSGRARGTADAGKLGDEAADVLGMLLIYCDRAGIDLEQAMQRKWLHWLEPKTAVP
                     SA"
     gene            complement(602..1459)
                     /locus_tag="VF686_04435"
     CDS             complement(602..1459)
                     /locus_tag="VF686_04435"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX8661092.1"
                     /translation="MNAQVLLADIHARAGAVDADHPEWQYLNLLRDILDNGAQRDDRT
                     GTGTLGVFGRQMRFDLAKGFPVLTTKKLHLRSIIVELLWFLRGETNIGWLKDNGVSIW
                     NEWADAEGELGPVYGKQWRSWAAPDGRVIDQITKLVEGLKTNPNSRRHIVSAWNPADV
                     DDMALPPCHCLFQFFVADGKLSCQLYQRSADVFLGVPFNIASYALLTMMVAQVCGLKP
                     GEFVHTFGDAHLYLNHVEQAELQLSREPRPAPTMTITTRNDLFAYRLEDFVLEGYEPW
                     PHIKAPVAV"
     gene            complement(1543..1716)
                     /locus_tag="VF686_04440"
     CDS             complement(1543..1716)
                     /locus_tag="VF686_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661093.1"
                     /translation="MDCARAYSRRRYGLIQPGVKRRKPVGVGSGKPITVKRDWAVKNR
                     AQLAQARRDRMRD"
BASE COUNT          304 a          621 c          614 g          315 t
ORIGIN      
        1 cgcccggcca cgaaggctcc cagactgacg gccggattga cgtggcagcc cgacaccgga
       61 ccgatgccat aggccatggc gataatggcg aaaccgaagg ccagggcgat gcccaactga
      121 ccgacatgat cgaacccgcc cagcacggcg gcgccgcacc cgaacaggac cagcaccaat
      181 gtgccgacga actccgctgc gaacttcttc accatggcaa cctccccgtt gctacggccc
      241 cgcttcacgc gggggcgagg gacggtgggc cgagtcttat ggtgacgcta ggcggaagga
      301 acggcggtct tcggctccag ccagtgcagc catttgcgct gcatcgcctg ttccaggtcg
      361 atgcccgccc ggtcgcaata gatcagcagc atgcccagca cgtctgcggc ctcgtcgccc
      421 agcttgccgg cgtcggccgt tccgcgggcc cggccgctca tgcgcagatg ttccgcggtc
      481 agctcgccca gctcttcctg gatcttcagc agggcccagt cggcggaccg ttcgatgccg
      541 tgctcggcgg cgtagatgtc ggagatgcgc aggacgtcgg cctgcagggt cttgaggtgc
      601 atcagaccgc gaccggagcc ttgatatgcg gccagggctc gtagccctcg agcacgaagt
      661 cctccagcct gtaggcgaac aggtcgttgc gcgtggtgat ggtcatggtc ggggcgggcc
      721 ggggctcgcg gctcagctgc agctcggcct gttcgacatg gttcagatac aggtgggcgt
      781 cgccgaaggt gtgcacgaat tcgccgggct tcaggccgca cacctgcgcc accatcatgg
      841 tcagcagggc gtaggaggcg atgttgaacg gcacgcccag gaacacgtcg gcgctacgct
      901 ggtagagctg gcagctcagc ttcccgtcgg cgacgaagaa ctggaacagg cagtggcagg
      961 gcggcagggc catgtcgtcg acgtcggccg ggttccacgc cgaaacgata tggcggcggc
     1021 tgttgggatt ggtcttcagc ccctcgacca gtttggtgat ctggtcgatc acccggccgt
     1081 ccggcgcggc ccacgatcgc cactgcttgc cgtagaccgg acccagctcg ccctcggcgt
     1141 cggcccattc gttccagatg ctgacgccgt tgtccttcag ccagccgata ttggtctcgc
     1201 cgcgcaggaa ccacagcagt tcgacgatga tcgaccgcag gtgcagcttc ttggtcgtca
     1261 ggaccgggaa acccttggcc agatcgaacc gcatctggcg cccgaagacg cccagcgtcc
     1321 cggtgcccgt ccggtcgtcg cgctgggccc cattgtcgag gatgtcgcgc agcaggttca
     1381 ggtactgcca ctcgggatga tcggcatcga ccgcgccggc gcgggcgtgg atgtcggcga
     1441 gaaggacctg ggcgttcatc ctgccagagt ggcgctgatt cggtcgcctt ggcaggcgcg
     1501 acctcggctt gctcacagaa acgtgccggg gcctgtgggc gatcagtccc gcatccggtc
     1561 ccgccgggcc tgcgccagtt gggcccggtt cttcaccgcc caatcccgct tgaccgtgat
     1621 cggcttgcca gagcccactc cgacgggctt tcggcgcttc actccgggct ggatcagccc
     1681 atagcggcgc cgcgaatagg cgcgcgcgca gtccatgcag tgacgatatg tgcgcgcggc
     1741 ataggaggcg tgccagttac ggccctcctg cagaacggtt tcgcactcac ggcaacggtg
     1801 caaaggcgat cagcccctga acccgtctgc gtccagaaac acttgctcct ccgg
//
LOCUS       DASVVN010000279         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_221267,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000279 DASVVN010000000
VERSION     DASVVN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221267"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VF686_04445"
     CDS             complement(<1..170)
                     /locus_tag="VF686_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX8661094.1"
                     /translation="MADGSPVTAFDPYAVRAQFPILSRSVNGRPLVYLDSAASAQKPR
                     AVIDAMTAAMEG"
     gene            complement(149..1177)
                     /gene="sufD"
                     /locus_tag="VF686_04450"
     CDS             complement(149..1177)
                     /gene="sufD"
                     /locus_tag="VF686_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX8661095.1"
                     /translation="MNAPVRIDLKDASSFPSKRVEAWKYSDLRRHLREAPAPSPSATV
                     PLVGGGPFETLGGEAMVFVNGRAVNETQLMVSGDRTVRLRLISRAEGTGHALTARISV
                     RAGAKLLLLETHEGEGSAYVAHNRLELDVARGAEVTRLVLVDEPEDAISITEARVRVE
                     AGGVYRQTILTTGAKLQRQETQVTHRAQGADVRLDGVYVLSDARQADLTSVVDHVAAD
                     GLTSQLTKGVVRDTARGVFQGKIIVERGADGTDARMGHHALILGERAEVDAKPELEIY
                     ADDVQCAHGNTVGNLDESALFYMQQRGLPADEARALLTEAFLIEVVDRIEHEGAREVV
                     REWLTDRL"
     gene            complement(1174..>1704)
                     /gene="sufC"
                     /locus_tag="VF686_04455"
     CDS             complement(1174..>1704)
                     /gene="sufC"
                     /locus_tag="VF686_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX8661096.1"
                     /translation="ERAAKGVFLSFQYPIEIPGVPALTFVRTALNAQKRARGEDEMSA
                     PAFLKMVKAASAALKMDFDMLKRPLNVGFSGGEKKRMEILQMALLEPSLLILDETDSG
                     LDIDALRIVAEGVNALRSPERAMLVITHYQRLLDYIKPDRVHVLAGGRIVASGGPELA
                     HQLEAEGYDKYLPEAA"
BASE COUNT          259 a          633 c          535 g          277 t
ORIGIN      
        1 ctgccctcca tcgccgccgt catggcgtcg atcaccgccc gcggcttctg cgccgaggcg
       61 gctgagtcca gatagaccag cggccggccg ttcaccgacc gcgacaggat cggaaactgc
      121 gcccggacgg cgtagggatc aaaggcggtc acaggcgatc cgtcagccat tcccgcacca
      181 cctctctcgc gccctcatgc tcgatccggt cgaccacctc gatcaggaag gcctctgtca
      241 gcaacgcccg cgcctcgtcg gccgggaggc cgcgctgctg catgtagaac agcgcgctct
      301 catccagatt gcccaccgta ttgccgtgcg cgcactgaac gtcgtcggcg tagatctcca
      361 gctcgggctt ggcgtcgacc tcggcgcgtt cacccaggat cagggcgtga tggcccatcc
      421 gcgcatcagt tccgtcggcg ccgcgctcga cgatgatctt cccctggaag acgccgcgcg
      481 ccgtgtcgcg caccacgccc ttggtcagct ggctggtgag cccatcggca gccacatgat
      541 cgaccaccga ggtcagatcg gcctgccgcg cgtccgacag cacatagacg ccgtccagcc
      601 gcacgtcggc gccctgcgcg cgatgcgtga cctgcgtctc ctgcctttgc agcttcgcgc
      661 cggtcgtcag gatggtctgg cgatagacgc cgcccgcctc gacccgcacc cgcgcttccg
      721 tgatcgagat ggcgtcctcg ggttcgtcga ccagcaccag ccgggtcacc tcggcgcctc
      781 gcgccacgtc cagctccagc cggttgtgcg ccacataggc cgagccctcg ccctcatgcg
      841 tctccaacag cagcagcttc gcgccggccc tcaccgagat gcgtgcggtc agggcgtgac
      901 ccgtcccctc cgcccgcgag atcaggcgca gccgcaccgt cctgtcgccc gagaccatca
      961 gctgggtctc gttcacggcc cggccgttga cgaagaccat ggcctcgccg cccagcgtct
     1021 cgaacggtcc gccgccgacc agcggcacgg tcgccgacgg cgaaggggcg ggcgcctcgc
     1081 gcagatgacg gcgcaggtcc gaatatttcc acgcctcgac ccgcttcgag gggaagctgg
     1141 aggcgtcctt cagatcgatg cggacaggcg cgttcacgcg gcttccggga ggtatttgtc
     1201 gtacccttcc gcttccagct gatgcgccag ttccgggccg cccgaggcca cgatccgccc
     1261 cccggccagc acatgcaccc ggtcgggttt gatgtagtcg agcagccgct gatagtgggt
     1321 gatcaccagc atggcgcgct cgggcgaacg cagggcgttg accccctcgg ccacgatgcg
     1381 cagggcgtcg atgtcgaggc cggaatccgt ctcatcgagg atcagcagcg acggttccag
     1441 cagggccatc tgcaggattt ccatccgctt cttctcgccg cccgagaagc cgacgttcag
     1501 cggccgcttg agcatgtcga agtccatctt cagcgccgcc gacgccgcct tgaccatctt
     1561 caggaaggcg ggcgcggaca tctcgtcctc gccgcgcgcg cgcttctgag cgttcagcgc
     1621 cgtgcgcacg aaggtcaggg ccgggacgcc gggaatctcg atcgggtact ggaacgacag
     1681 gaagacgccc ttggccgccc gttc
//
LOCUS       DASVVN010000280         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_404922,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000280 DASVVN010000000
VERSION     DASVVN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404922"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VF686_04460"
     CDS             complement(<1..1291)
                     /locus_tag="VF686_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169869.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8661097.1"
                     /translation="MKAVTLAGETDFDGWRWAARGFRQAGVAPGEAVFRVGDAGGGLF
                     DDVLHLPAVEPVFAVSKAFVDLARDVVLHRSADRFELLYRLLWRLRDEPDLMRVVSDV
                     DVADALERAKNVSRASHKMKAFVRFRQIEDGEGERWVAWFEPAHRVVEKTAPFFVRRF
                     TTMRWSILTPDGSAVWDGDALGFGPPATRDMAPAEDGIEEFWKTYYASTFNPARLKTK
                     TMQGEMPKRYWKNLPEASLIPELVARSAVRTDAMVATPAAQPNPRLAKTLSRMNRDHS
                     FDEGFVPSTLEVLGQAVQACRRCPLWRDATQGVCGEGPRRARLMIVGEQPGDQEDLAG
                     RPFVGPAGGVLDTALAEAGIDREDVFVTNAVKHFKHEPRGKRRIHKTPLVSEVSACRW
                     WLNAERRLVKPKVILTLGATAALGVLNRKVAVTKERGE"
     gene            complement(1291..>2138)
                     /locus_tag="VF686_04465"
     CDS             complement(1291..>2138)
                     /locus_tag="VF686_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8661098.1"
                     /translation="KLRTEHDFRGYIHLKLIPEADPRLVEEAGLYADRLSANIELPRD
                     EALQRLAPQKDVGVIKKAMGQVRLKVDAGRPQKKDRAKPPRFAPAGQSTQLIIGADGA
                     PDTEIIDRSASLYGGYGLRRVYYSAFSPIPDSSSALPLSKPPLMREHRLYQADWLMRF
                     YGFSPPEIGEATVDGMLDLAIDPKLAWALNSRASFPVDVNTGPREMLLRVPGLGVKSV
                     DKILQVRRWRTLRLEDIGRLCRGIDKVRPFITALDWTPGGLTDAANLRARIAPGPKPV
                     QLSLF"
BASE COUNT          341 a          794 c          651 g          352 t
ORIGIN      
        1 cctctccgcg ctcctttgtc accgcgacct tgcggttcaa cacgcccaac gccgcggtgg
       61 ctcccagcgt caaaatcacc ttcggcttca ccagccgccg ctcggcgttc agccaccagc
      121 ggcaggcgct gacctcgctg accaacggcg tcttgtggat gcgccgcttg ccgcgtggct
      181 cgtgtttgaa gtgtttcacg gcgttggtga cgaagacgtc ctcgcggtcg atcccggctt
      241 ccgccagcgc cgtatcgagc acgccgccgg cgggtcctac gaacggccgg cccgccagat
      301 cctcctgatc gcccggctgt tcgcccacga tcatcagccg cgcccgcctc ggtccttccc
      361 cgcagacgcc ttgtgtagca tcgcgccaca gggggcagcg acggcaggcc tgcaccgcct
      421 gccccagcac ttccaaagtc gagggaacaa agccctcgtc gaacgaatgg tcgcggttca
      481 tccgcgagag cgtcttcgcc agacgcgggt tcggctgggc ggcgggcgtg gcgaccatgg
      541 cgtctgttcg aaccgccgac cgcgccacca gttccgggat cagcgaggct tccggcaggt
      601 tcttccagta ccgcttgggc atttcgccct gcatggtctt cgtcttcagc cgcgccgggt
      661 tgaaggtcga ggcgtagtag gtcttccaga actcctcgat cccgtcctcc gccggcgcca
      721 tatcgcgcgt cgccggcggg ccaaagccca gcgcgtcccc gtcccagacc gcgctgccgt
      781 ccggcgtcag gatcgaccac cgcatggtgg tgaagcgccg gacaaagaac ggcgccgtct
      841 tctccaccac cctgtgcgcc ggctcgaacc aggcgaccca ccgctccccc tcgccatcct
      901 cgatttgccg gaaccggacg aaggccttca tcttgtggct ggcccggctg acgtttttgg
      961 cccgctcgag agcgtcggcc acgtcgacgt cggagacgac cctcatcaga tccggctcgt
     1021 cccgcagccg ccacagcagc cggtacaaca gttcgaaccg gtcggccgag cgatgcagca
     1081 ccacatcccg cgccagatcg acgaacgctt tcgacacggc gaataccggt tccacggcgg
     1141 gcagatgcag gacgtcgtcg aacaggccgc caccagcatc gcctaccctg aaaacggcct
     1201 cgcccggggc gacccccgcc tgccggaacc cccgcgccgc ccaacgccag ccgtcaaagt
     1261 ccgtttcgcc cgcaagcgtc acggccttca tcagaacagg ctcagctgga ccggctttgg
     1321 cccgggcgcg atacgcgccc gcaaattcgc cgcgtcggtc agcccgcccg gcgtccagtc
     1381 gagggcggtg atgaatggcc gtaccttgtc gatcccccgg cacagccggc cgatatcctc
     1441 cagccgcagg gtccgccagc gccgcacctg aaggattttg tcgaccgact tcacgccaag
     1501 gcccggcacc cggagcagca tctcgcgcgg ccccgtattc acatccaccg ggaacgacgc
     1561 gcggctgttc agcgcccagg ccagtttggg atcgatggcc agatccagca tgccgtccac
     1621 cgtcgcctcg ccgatctcgg gcggcgagaa gccgtagaac cgcatcagcc agtcggcctg
     1681 atacagccgg tgctcgcgca tcagcggcgg ctttgacagc ggcagggcgc tcgaggaatc
     1741 cggtatgggg ctgaaggccg agtaatagac ccggcgcagg ccgtagccgc cgtacagcga
     1801 cgccgatcgg tcgatgatct cggtgtccgg cgctccgtca gcgccgataa tcagttgggt
     1861 cgattgcccc gccggggcga acctcggcgg tttcgcccga tccttcttct gaggccgccc
     1921 ggcgtccacc ttcagacgaa cctgacccat ggccttcttg atgacgccca catccttttg
     1981 cggcgccagc cgctgcagcg cctcgtcgcg cggcagttcg atattggccg acagccggtc
     2041 ggcgtagaga cccgcctcct cgacgagacg gggatcagcc tccgggatca gcttcaggtg
     2101 gatgtagccc cggaagtcat gctcggtgcg cagcttct
//
LOCUS       DASVVN010000281         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_221286,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000281 DASVVN010000000
VERSION     DASVVN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221286"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF686_04470"
     CDS             complement(<1..215)
                     /locus_tag="VF686_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8661099.1"
                     /translation="MLTTLIVIAVIVAAVLFVIVGAYNKLVALDQRADQSFADIDVQL
                     KQRQDLIPNLVETVKGYATHERGTLDAV"
     gene            complement(448..2649)
                     /gene="parC"
                     /locus_tag="VF686_04475"
     CDS             complement(448..2649)
                     /gene="parC"
                     /locus_tag="VF686_04475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX8661100.1"
                     /translation="MTVHTPPPEGGRIVDEPMSEALSRRYLAYALSTITNRALPDVRD
                     GFKPVHRRIMYAMHQMRLNPQAAARKCAKVVGEVMGGYHPHGDASIYDALVRLAQDFS
                     QRYPLVDGQGNFGNIDGDNAAAMRYTECKLTPAAVLLMEGIDQDSVDFKATYDDQDEE
                     PIVLPAGFPNLLANGSSGIAVGMATSIPPHNVGELIDACQVLLERPDATTEELATIVP
                     GPDFPTGGVAVEGHASILDAYETGRGGVRLRARWETEDLGRGVWRIIVTEMPYQVQKS
                     KLIADLADLNEHKKAPLLGDVRDESAEDIRLVIEPKSRTVEPEVLMESLFKLSDLEVR
                     FPINMNVLDATGTPRVKGLKACLQAFLDHRRDVLVRRAHWRIERVEKRLHLLEGLRIV
                     FLNLDEVIRIVREEEQPKAVLIARFGLSEFQADFILDTRLRQLARIEEMTIENEYKAL
                     SDELAALQSLSSSPVKQWKQVGKDLETVRKALVSPRRTTIAEAVDSAAFAAVEAFIPR
                     EAITVILSERGWLRAAKGKVEDPSELKFKEGDSLAFLVPGYTTDKLLVAASDGRIFTV
                     GADKLASGRGHGEPLRLMIDLDEKAEIVNLLMHQPGGKLLIASKAGYGFIAPDDELLA
                     QKRGGKQVLNGDMRAALRVTGDHVAAIGDNIKALIFPVAELPEMGRGKGVKLQSYKQG
                     GLADVTVFDSAHGPEWVDGGARRRNWPDWKDWLGKRAGAGRQGPRGLRKFR"
     gene            2749..>2963
                     /locus_tag="VF686_04480"
     CDS             2749..>2963
                     /locus_tag="VF686_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8661101.1"
                     /translation="MQTAVTRRLGLDSPIIQAPMAGTSTPELAAAVSNAGALGSIALG
                     AIDAEAARRDLRRTRTLTARPFNVNLF"
BASE COUNT          449 a          964 c         1026 g          524 t
ORIGIN      
        1 acggcgtcca gggtgccgcg ctcgtgcgtg gcgtagccct ttacggtctc gaccagattg
       61 gggatcaggt cctgacgctg cttcagctgc acgtcgatgt cggcgaacga ctggtcggcg
      121 cgctggtcga gagcgaccag cttgttgtag gcgccgacga tgacgaacag caccgcggcg
      181 acaatgacgg cgatgacgat cagggtggtc agcattgcgg tctccaacgt cggtcgatga
      241 tcgaccgtca gcgattatcg ggcgccatgg tcacgctcga tagtgaaatc ggtgcaacct
      301 cgctgtcgtc atcccggcga aggccgggac ccagcggcgc cgaaggcgat ctgttcaccc
      361 acgatgggtc gagcagccgt gtccgcgaca ggttcgcgct ctcgcgcgcc actgggtccc
      421 ggcctacgcc gggatgacgg caggaaatca tctgaacttt ctcaaccccc gcgggccctg
      481 acgccctgcc ccggcgcgct tgccgagcca gtccttccag tcgggccagt tgcggcggcg
      541 ggcgccgcca tcgacccatt cggggccgtg ggcgctgtcg aagactgtca cgtcggccag
      601 tcctccctgt ttgtaggact gcagcttcac ccccttgccg cggcccatct cgggcagttc
      661 ggcgacaggg aagatcaggg ccttgatgtt gtcgccgatg gccgcgacat ggtcgcccgt
      721 gacacgcagg gcggcgcgca tgtcgccgtt cagcacctgt ttgccgcccc gcttctgggc
      781 cagcagctcg tcgtcggggg cgatgaagcc gtagccggcc ttcgaggcga tcagcagctt
      841 gccgccgggc tggtgcatca gcaggttcac gatctcggcc ttttcatcga ggtcgatcat
      901 caggcgcaac ggctcgccgt gaccgcggcc cgaggccagc ttgtcggcgc caacggtgaa
      961 gatgcggccg tccgatgccg ccaccagcag tttgtcggtc gtatagccgg gcacgaggaa
     1021 ggccaggctg tcgccctcct tgaacttcag ttcggacggg tcctccacct tgcccttggc
     1081 cgcccggagc cagccgcgtt cggacaggat gacggtgatg gcctcgcgcg ggatgaaggc
     1141 ctcgaccgcg gcgaaggcgg cgctgtcgac ggcctcggcg atggtggtgc ggcgcggcga
     1201 gaccagcgcc ttgcgcacgg tctcgaggtc cttgccgacc tgtttccact gcttgaccgg
     1261 cgaggacgac agcgactgca gcgccgcaag ttcgtcagat agagccttgt attcgttctc
     1321 gatcgtcatc tcctcgatcc gcgccagctg gcgcagtcgg gtgtcgagga tgaagtcggc
     1381 ctggaactcg gagaggccga agcgggcgat caggacggcc ttgggctgtt cctcctcgcg
     1441 aacgatgcga atgacctcgt ccaggttcag gaagacgatc cgcaggcctt ccagcaggtg
     1501 cagccgcttc tcgacccgtt cgatgcgcca gtgcgcgcgg cggaccagaa cgtcccggcg
     1561 gtgatcgagg aaggcctgaa ggcaggcctt cagccccttg acgcgtgggg tgccggtggc
     1621 gtccagcacg ttcatgttga tcgggaagcg gacctcgagg tccgagagct tgaacaggct
     1681 ctccatcagg acctcgggct cgaccgtgcg gctcttcggc tcgatgacca gccggatgtc
     1741 ctcggccgac tcgtcgcgca cgtcgcccag cagcggcgcc ttcttgtgct cgttcaggtc
     1801 ggcgaggtcg gcgatcagct tcgacttctg cacctggtac ggcatctcgg tgacgatgat
     1861 gcgccagacg ccgcggccga ggtcctcggt ctcccagcgg gcgcgcagac gcacgccgcc
     1921 gcggcccgtc tcataggcgt cgaggatgga ggcgtggcct tccacggcga cgccgccggt
     1981 cgggaagtcc gggcccggga cgatggtcgc cagttcttcc gtcgtggcgt caggccgctc
     2041 cagcagcacc tggcaggcgt cgatcagctc gccgacattg tgcggcggga tcgaggtggc
     2101 catgccgacg gcgatgccgg acgagccgtt ggccagcagg ttggggaagc cggcgggcag
     2161 gacgatcggc tcttcgtcct gatcgtcgta ggtggccttg aagtccaccg agtcctggtc
     2221 gatgccctcc atcagcagga cggcggccgg cgtcagcttg cactcggtgt agcgcatggc
     2281 cgcggcgtta tcgccgtcga tgttgccgaa attgccctgc ccgtcgacca agggataacg
     2341 ctgggagaag tcctgggcga ggcggaccaa ggcgtcgtag atcgaggcgt cgccgtgcgg
     2401 gtgatagccg cccatgacct cgccgacgac cttggcgcat ttgcgcgccg ccgcctgcgg
     2461 gttcagccgc atctggtgca tggcgtacat gatgcgccgg tgcacgggct tgaagccgtc
     2521 gcgcacgtcc ggcagggcgc ggttggtgat ggtcgacagc gcataggcca gatagcggcg
     2581 cgacagcgcc tcgctcatcg gctcgtctac gatgcgtccg ccttccggcg gcggggtgtg
     2641 aacggtcatg gggtccgaat cggcggtctg gagcgtgaag tctagcgcag ccttgcattg
     2701 gcggtgtgga tagacagacc tatctgcgga tcaggccgga gtatccccat gcaaaccgca
     2761 gtgacgcgcc gccttggcct cgacagcccg atcatccagg cgccgatggc tggcacgtcg
     2821 acgccggagc ttgcagccgc ggtttcgaac gccggagcgc tgggctccat tgccttgggc
     2881 gccatcgatg cggaggcggc ccgccgggac ctgcggcgca cccgaacgct cacagcccgg
     2941 cccttcaacg ttaatctgtt ctg
//
LOCUS       DASVVN010000282         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_299493,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000282 DASVVN010000000
VERSION     DASVVN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299493"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF686_04485"
     CDS             complement(<1..378)
                     /locus_tag="VF686_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX8661102.1"
                     /translation="MSAQTETQETEAQSDGPLLDLTDAGVKKFIKQAKARGYVTMEDL
                     NKVLPSEEVTPDAIEDTLAMLSEMGVNVVEAEEDAAETTEKEGGEVVERAETNVAETP
                     AKAAYDRTDDPVRMYLREMGSVEL"
     gene            complement(448..>2099)
                     /gene="dnaG"
                     /locus_tag="VF686_04490"
     CDS             complement(448..>2099)
                     /gene="dnaG"
                     /locus_tag="VF686_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX8661103.1"
                     /translation="QETERLSFVEAVQRLAGEAGMALPAEDPQAAEREAKKQGLTDWM
                     ELAQKWFAANLRRSPGKAAREYLEKRGLPEDQWERFGLGYAPNDREGLKNALVQRGAK
                     PGDLVEMGMLIAPEGGGAPYDRFRDRLMFPILDARGRIVSFGGRAMNPDDRAKYLNGP
                     ESPLFHKGATLYGLPEARRILGAESKSDQAIVVVEGYMDVIACHRAGIPAVAPMGTAL
                     TEEQMERLWRVSHEPVLCFDGDAAGRRAAYRAIERALPQLKSGRSFRFALLEGGQDPD
                     DILRDKGAPALRQAMAATRPFAEVLFQKEAEVEPLDSPERKAGLKGRLRQAASAIQDK
                     DLAEQYRRELFDRFDALFPARRAPAGQPATGPGGRWRPGPPPKLGQTAEGAQAMQSLS
                     RSIEPVAAALAHGAVDDPERMDNHLEAIAAHGFGDPALDGLAQEMVRLRFSGQDLDSA
                     ALRRHLAQSGHGVLMHEVEKAAAKSGAPFLATDKPLGEARIRWSQAFDALTRVAALED
                     ALTSARGVPGQDDAFRRLKAERDALRRAIKTGEIWEDSAGS"
BASE COUNT          289 a          762 c          677 g          371 t
ORIGIN      
        1 cagttcgacc gagcccatct cgcgcagata catgcgcacc gggtcatcgg tgcggtcata
       61 ggcggccttg gccggagtct cggcgacgtt ggtctcggcg cgctcgacga cttcgccgcc
      121 ttctttttca gtggtctcgg ccgcgtcttc ctcggcctcc acgacattga cgcccatctc
      181 ggacagcatc gccagggtgt cctcgatcgc gtcgggcgtc acctcttcgg acggcaggac
      241 cttgttcagg tcctccatcg tgacatagcc ccgcgccttg gcctgtttga tgaatttctt
      301 caccccggcg tcggtgaggt cgagcagcgg accgtcgctc tgggcttcgg tttcctgcgt
      361 ctcggtttga gcgctcatat agtctgttct ccgggtggag cggctgatca ggccgtccgc
      421 caatacaacg tgtggcgggg tcgttggtta cgaacccgcg ctgtcttccc aaatctctcc
      481 ggttttgatc gcacgacgca gcgcatcccg ctcggctttc agccggcgaa acgcgtcgtc
      541 ctgccccggc acgccacgcg ccgaagtcag ggcatcctcc agcgccgcca cgcgagtgag
      601 cgcgtcgaac gcctgcgacc atcggatccg cgcctcgccg aggggcttgt ctgtggccag
      661 gaagggcgcg ccggactttg ccgccgcctt ctcgacctcg tgcattaaaa cgccgtgacc
      721 cgattgcgcc agatggcgac gcagcgcagc ggagtcaagg tcctgacccg agaagcgcag
      781 acgaaccatc tcctgcgcca gcccgtccag ggccggatcg ccgaacccgt gagcggcgat
      841 ggcctccagg tgattgtcca ttcgctcggg atcgtcgacc gcgccatggg ccagggcggc
      901 ggcgacgggc tcgatcgatc gggacagcga ctgcatcgcc tgcgccccct ccgccgtctg
      961 tcccagcttg ggcggcggcc cgggacgcca gcgcccgccc ggccccgtcg cgggctggcc
     1021 ggccggggcg cggcgcgccg ggaacagggc gtcgaagcgg tcgaacagtt cgcggcgata
     1081 ctgttccgcc agatccttgt cctgaatggc cgatgcggcc tgacgcagcc ggcctttcag
     1141 gcccgccttg cgctcgggcg aatccagcgg ctcgacctcg gcctccttct ggaacagcac
     1201 ctcggcgaag ggccgggtcg cggccatggc ctgacgcagg gccggggcgc ccttgtcgcg
     1261 caggatgtcg tccgggtcct gaccgccctc caacagggcg aaccggaacg accggcccga
     1321 cttcagttgc ggcagagccc tttcgatcgc gcggtaggcc gcccgtcgtc ccgccgcatc
     1381 cccgtcgaaa cacagcaccg gctcgtgcga cacgcgccac agccgttcca tctgctcctc
     1441 ggtcagggct gtgcccatcg gtgcgacggc ggggatgccc gcccggtggc aggcgatcac
     1501 atccatatag ccctcgacca ccacaatggc ctgatcgctc ttcgactccg cgccgaggat
     1561 gcgtcgcgcc tcgggcaggc cgtacagggt cgcgcccttg tggaagagcg ggctctctgg
     1621 cccgttcaga tatttggccc ggtcgtccgg gttcatcgcc cggccgccga agctgacgat
     1681 gcggccgcgg gcgtcgagga tggggaacat cagccggtcg cggaaccggt catagggggc
     1741 gccgccgcct tccggggcga tcagcatccc catctcgacc agatcgcccg gtttggcgcc
     1801 gcgttggaca agggcgtttt tcaacccttc gcgatcgtta ggcgcatagc cgagaccgaa
     1861 ccgctcccac tggtcctcgg gcagtccgcg cttttccaga tactcgcgcg cggccttgcc
     1921 cggcgagcga cgcaggttgg ccgcgaacca tttctgggcc agctccatcc agtccgtcag
     1981 gccctgtttc ttcgcctcgc gctcggccgc ctgcgggtcc tcggccggca gggccattcc
     2041 cgcctccccc gccagccgct gcacggcttc gacgaaggac agccgctcgg tctcctgca
//
LOCUS       DASVVN010000283         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_324840,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000283 DASVVN010000000
VERSION     DASVVN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324840"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VF686_04495"
     CDS             <1..1019
                     /locus_tag="VF686_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX8661104.1"
                     /translation="ETFRTRELMSQQRMEAQLKPVADTLKLFQEHVAALEKARNEETG
                     GLKEQLALLMTASTATRDEARRLTEALRGNTGRRGRWGEQTCRNVLEAAGMAGRFDFE
                     EQTSSADDAGRQQRPDFVVRLPGGGMFVIDAKVSLAFGDATDGDEEAQARAASLRTAT
                     SMKTHVRQLSSKAYQDQFKPSPDFVAMFVPGDAFLAAALDHEPDLMTTAMASRVVIVT
                     PTTLFALCKAVAYGWRAEEQARNAEDVAKLGKELYKRLSVMGGHAAAVGRALDTAVGK
                     YNQFVGSLESQVMVSARRFEDLQVDHEGKDLPELTPVDQSPRTLSRPELVTGGNAAPA
                     LPDV"
     gene            1099..1560
                     /locus_tag="VF686_04500"
     CDS             1099..1560
                     /locus_tag="VF686_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661105.1"
                     /translation="MSKRLWPVIGKSILGLIALLALIFVVGIAVGFLSAHGTVSAERA
                     VVPVMTVFAVAMMIGGMAVTVAWMRAIDEAAREAHKAAWFWGGCSGMAVGGVGLVLAS
                     LPQAETLQFAALDGRTDPAAYMAAGAFAMMSLMTLGYIVVWVWWWLARTRG"
     gene            1563..>1716
                     /locus_tag="VF686_04505"
     CDS             1563..>1716
                     /locus_tag="VF686_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8661106.1"
                     /translation="MNNRLKVLRAERDWSQADLADALGVSRQTVNALETGRYDPSLPL
                     AFRIAKV"
BASE COUNT          272 a          627 c          555 g          262 t
ORIGIN      
        1 acgagacctt ccgcacccgc gaactgatgt cccagcagcg gatggaggcg cagctcaagc
       61 cggtcgcgga caccctcaaa ctgttccagg aacacgtcgc ggcgctcgaa aaggcccgta
      121 acgaggagac cggcggcctc aaggaacagc tggccctgct gatgaccgcc tccaccgcca
      181 cgcgcgacga ggcccgccgc ctgaccgagg ccctgcgcgg caacaccggc cggcgcggcc
      241 gctggggcga acagacctgc cgtaacgtgc tcgaggccgc aggcatggcc gggcggttcg
      301 acttcgagga acagacctcc tcggccgacg acgccggccg gcagcagcgc cccgatttcg
      361 tggtccgcct gccgggcggc ggcatgttcg tgatcgacgc caaggtgtcg ctggccttcg
      421 gcgacgcgac cgacggcgac gaggaggcgc aggccagggc cgcctccctg cgcaccgcga
      481 ccagcatgaa gacccacgtc cgccagctgt cgtccaaggc ctatcaggac cagttcaagc
      541 ccagccccga cttcgtggcc atgttcgtgc ccggcgacgc cttcctggcc gccgccctcg
      601 accacgagcc cgacctgatg accactgcca tggcctcgcg cgtggtcatc gtcaccccga
      661 ccaccctgtt cgccctctgc aaggccgtcg cctacggctg gcgcgccgag gagcaggccc
      721 gcaacgccga ggacgtcgcg aaactgggca aggaactcta caaacggctg tcggtcatgg
      781 gggggcacgc cgccgccgtc ggccgcgcgc tcgacaccgc cgtcggcaaa tacaaccagt
      841 tcgtcggctc gctggagagc caggtcatgg tctcggcccg ccgcttcgag gatttgcagg
      901 tcgaccacga gggcaaggac ctgcccgagc tgactccggt cgaccagtcg ccccggacac
      961 tcagtcggcc cgaactggtc accggcggaa acgctgcgcc ggccctgccg gatgtctgaa
     1021 ttccccgtga caaggacgct tgacatccgc ccgggccgat gtaaaacacc cttgtcttag
     1081 cgccgagggg aagctgtcat gtcgaaacgt ctctggcccg tgatcgggaa atccattctc
     1141 ggtctgatcg ccctgctggc tctgatattc gtcgtgggca tcgccgtcgg cttcctcagc
     1201 gcccacggta cggtgtcggc tgagcgggcg gtcgttcccg tgatgaccgt cttcgccgtc
     1261 gcgatgatga ttggcgggat ggctgtcacc gtcgcctgga tgcgtgccat cgacgaggcc
     1321 gcgcgcgagg ctcacaaggc ggcctggttc tggggcggct gcagcggcat ggccgtcggc
     1381 ggcgtgggcc ttgtcctcgc cagcctgccg caggccgaaa cccttcagtt cgccgccctg
     1441 gacggccgca ccgacccggc ggcctatatg gcggccggcg ccttcgccat gatgtcactc
     1501 atgacgctcg gctacatcgt ggtctgggtc tggtggtggc tggcccggac gcgaggctga
     1561 gcatgaacaa ccgcctcaag gttctccgcg ccgaacgcga ctggagccag gccgatctcg
     1621 ccgacgccct cggcgtctcg cgccagaccg tcaacgccct cgaaaccggc cgctacgacc
     1681 cctccctccc gctggccttc cgcatcgcca aggtgt
//
LOCUS       DASVVN010000284         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_88653, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000284 DASVVN010000000
VERSION     DASVVN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88653"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF686_04510"
     CDS             complement(<1..325)
                     /locus_tag="VF686_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8661107.1"
                     /translation="MSEANALPVNAIEVRGLEKTYAGTKKAPPKTALRGVDLVIPRGS
                     MFGLLGPNGAGKSTLINILAGVVNKTGGTASIWDRDIDRQPRDARAALGVVPQEIVAD
                     VFFTPR"
     gene            490..744
                     /locus_tag="VF686_04515"
     CDS             490..744
                     /locus_tag="VF686_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661108.1"
                     /translation="MTRTKMIGWTAFAACILGYGAFTAIVVLNRMGELEMRETLLFGG
                     PAAVIGEIGLWVAAGCLGWSLFKRRKALFDRVFRKGAPTV"
     gene            919..1158
                     /locus_tag="VF686_04520"
     CDS             919..1158
                     /locus_tag="VF686_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX8661109.1"
                     /translation="MPPRHPDAIIPPPEDIVVPTKRVACDGGGGALGHPLVYMDMGGD
                     DFVECAYCDRRFVLSAHPKPEDEYFSPAARGPEAH"
     gene            complement(1161..1820)
                     /locus_tag="VF686_04525"
     CDS             complement(1161..1820)
                     /locus_tag="VF686_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8661110.1"
                     /translation="MTLRLYSHPYSGYGQKAATALYELGLPFELRLIQHDDEAVMAEF
                     RAVSPFGKMPAVVDTEAGRTLFESSIVIEYLDRLTGGGRLIPTDPEAALEARFIDRII
                     DTYVGGPMNRIVLNKFRPEGQADDFGAAQDMEVVATAWDLLEARLADGRTWGAGESFT
                     LADCAAAPILTYARRLVPFGDRPLLKAWHGRLMARPSFVRALEDAAPFGDLMPAPAAE
                     D"
     gene            complement(1817..>2216)
                     /locus_tag="VF686_04530"
     CDS             complement(1817..>2216)
                     /locus_tag="VF686_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8661111.1"
                     /translation="AFSDREIKQRWMGCEEVSAPVIERLDFEVGGREISRGGGQDGAE
                     HLFDGRFLDIVPDRRIVFAFVMHVGGRKISASLGTAEFAPDGDGTLLTFNEQGVYLGE
                     DGWTEREEGTAAGLNQLDAWLASQHERASA"
BASE COUNT          363 a          755 c          711 g          387 t
ORIGIN      
        1 cgcgcggggt gaagaagacg tcggcgacga tctcctgcgg cacgacgccc agggcggcgc
       61 gggcgtcccg tggctgcctg tcgatatcgc ggtcccagat cgaggccgtg ccgccggtct
      121 tgttcacaac cccggcgagg atgttgatca aggtcgactt gcccgcgccg ttggggccca
      181 gaagcccgaa catcgaaccg cggggaatga ccaggtcgac gccgcgcaac gccgttttcg
      241 gcggagcctt cttcgtcccg gcgtaggtct tctccagtcc gcgcacctcg atggcgttga
      301 cgggcagggc gttcgcttcg gacatgcagg ctttccgttg gactagccgg cctaggtagg
      361 cttgccgacc gctgccgcca agaccgcggg ccatctcccc gggattgatg tcaacgacgc
      421 ttgacacaat acgcttagat gtctagcgtg cttgtcatta tgaacagctt gcttgacgga
      481 accaacacca tgacccgcac gaagatgatt ggctggacgg cattcgccgc ctgtatcctc
      541 ggctatggag ccttcacagc gattgtcgtc ctgaaccgga tgggcgagct ggagatgcgc
      601 gaaaccctgc tgtttggcgg accggccgcc gtgatcggcg agatcggtct gtgggtcgcc
      661 gccggctgcc tgggctggtc cctgttcaaa cggcgcaagg ccctgttcga ccgggtcttc
      721 cgcaaaggcg ccccgacggt ttgacgccgg acccccgtcc gccgaagcga aatcggcctt
      781 gatggacgcc agtccatcaa ggctcggatt tcgctccggt ttgttctgga ccattttttg
      841 gattcggatc gtttcgatcc gaagcaaaaa tggtctaggg aatcggtccc gccgcttctg
      901 cagttccgag atcgcgccat gccgccccgt caccccgacg ccatcatccc gccgccggaa
      961 gacatcgtgg tccccacgaa acgggtcgcc tgcgacggtg gcggcggagc cttgggccac
     1021 ccgctggtct atatggacat gggcggcgac gatttcgtcg aatgcgccta ttgcgaccgc
     1081 cgcttcgtgc tgagcgccca tccgaagccc gaagacgaat acttcagccc ggcagcccgc
     1141 ggtcccgagg cgcactgacc tcagtcctcg gcggccggcg caggcatcag gtcgccgaac
     1201 ggcgccgcat cctccagtgc gcggacgaac gacggccgcg ccatcagccg cccatgccag
     1261 gctttcagca gcggccggtc gccaaacggg accagccgcc gggcgtaggt caggatcggc
     1321 gccgcggcgc agtcggccag ggtgaagctc tcgcccgcgc cccaggtccg gccatcggcc
     1381 agccgcgctt ccagcaggtc ccaggcggtg gcgacgacct ccatgtcctg cgccgcacca
     1441 aagtcgtcgg cctggccctc gggacggaat ttgttgagca cgatccggtt catcggtccg
     1501 ccgacatagg tgtcgatgat ccggtcgatg aagcgcgcct caagggcggc ttccggatcg
     1561 gtcggaatca gccggcctcc gcccgtgagc cggtccagat attcgatcac tatgctggac
     1621 tcgaacaggg tccggcccgc ctcggtgtcg accacagccg gcatcttgcc gaagggcgag
     1681 accgcgcgga actcggccat gaccgcttcg tcgtcgtgct ggatcagcct cagctcgaac
     1741 ggcaggccca gttcgtacag cgccgtcgcc gccttctggc cgtagccgga gtagggatgg
     1801 gaatagagac gcagggtcat gcggaggccc tctcatgttg cgacgccagc caggcgtcca
     1861 gttgattcag accggccgcc gtaccctcct cgcgctcggt ccagccgtcc tcgccgagat
     1921 agacgccctg ctcgttgaag gtcagcagcg tcccgtcgcc gtccggcgcg aactcggcgg
     1981 tccccagcga ggccgaaatc ttccggccgc ccacgtgcat gacgaaggcg aagacgatcc
     2041 ggcggtcggg gacgatgtcc agaaaccggc cgtcgaacag atgttccgcg ccgtcctgac
     2101 cgccgccccg gctgatctcg cggccgccga cttcgaagtc gagccgttcg atcaccggag
     2161 cgctgacctc ttcgcagccc atccagcgtt gtttgatttc gcgatcggag aaggcg
//
LOCUS       DASVVN010000285         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_114947,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000285 DASVVN010000000
VERSION     DASVVN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114947"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1226
                     /locus_tag="VF686_04535"
     CDS             243..1226
                     /locus_tag="VF686_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8661112.1"
                     /translation="MDDRFEPRAEEIADQERKIQAGIDAKAGESGEQKEKPVQAGARK
                     YPEPPFPEQHQTKPGDEAALDPAPMYDAPFWKGSGKLDGMAAIITGADSGIGRAVAVL
                     FAREGCDVAICHLDEDTDAAGTKAAVEAEGRRAIVLQGDAADPAFSEAAVKATLDAFG
                     RLDVVVPNAAFQEHVEALEDLTLEHFDRTLKTNLYGYFNLVKAAVPHLKPGGSIVMTG
                     SVTGIMGNENLLDYSMTKGGIHAFARSLGTHLSQKGIRVNVVAPGPVWTPLNPADQQP
                     EKVAKFGSQTVMKRPAQPEEIAPAFVFLASPQMSSYITGEILPIIGGYGGG"
     gene            complement(1229..1516)
                     /locus_tag="VF686_04540"
     CDS             complement(1229..1516)
                     /locus_tag="VF686_04540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8661113.1"
                     /translation="MTEFRDVRDEQRFEQGFTDPEGQLRTVFADYATQGSTRIILHVE
                     ADPALRGSGAAGQFMQALAEHARAEELKLSPRCSYAVAWLKRHREYDDVVA"
     gene            complement(1632..>1814)
                     /locus_tag="VF686_04545"
     CDS             complement(1632..>1814)
                     /locus_tag="VF686_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661114.1"
                     /translation="TIGLWILIGVCVASIAAAPMLGLVLVPAAIVLGLGLLFLTVWFT
                     IKSIIGLINLVGDKAP"
BASE COUNT          328 a          650 c          568 g          268 t
ORIGIN      
        1 cgaaccgcgc cgccagcgcc agcatcagca gcggcaggaa ggtgcgcagc cccgtcgccg
       61 acgccagtcc cagccccagc agggcgggca gcacccaggt ctgcagcggc cccgcggcgg
      121 cgctcacggt ggacaggtcg aggtcgggca tggggcggct ccgtttcgtc gaacatgaca
      181 atgctcggga acggttgcgc gctccatcgg ctttttcctc tgtccagaga gaggagcccg
      241 ccatggatga ccgtttcgaa ccccgcgccg aagagatcgc ggatcaggag cgaaagattc
      301 aggccgggat cgacgccaag gctggcgaat cgggcgagca gaaagagaag cccgtccagg
      361 ccggtgcgcg caaatatccc gagccgccct tccccgaaca gcaccagacc aagcccggcg
      421 acgaggccgc tctcgaccct gccccgatgt atgacgcccc cttctggaag ggctcgggca
      481 agctggacgg catggccgcc atcatcaccg gcgcggacag cggcatcggc cgcgccgtag
      541 ccgtcctgtt cgcccgcgag ggctgcgacg tcgccatctg ccacctcgac gaggacacgg
      601 acgccgccgg gacgaaagcc gccgtcgagg ccgagggccg cagggccatc gtgcttcagg
      661 gcgacgccgc cgatccggcc ttctccgagg ccgcggtcaa ggcgaccctg gacgccttcg
      721 gccgcctcga tgtcgtcgtc cccaatgccg ccttccagga gcatgtcgag gcgctggagg
      781 atctgaccct cgagcatttc gaccgcacgc tgaaaaccaa tctctacggc tatttcaacc
      841 ttgtgaaggc ggcggttccg cacctgaagc ccggcggctc catcgtcatg accggctcgg
      901 tcaccggcat catgggcaat gagaacctgc tcgactattc gatgaccaag ggcggcattc
      961 acgccttcgc ccgctcgctg ggcacgcatc tgtcgcagaa gggcattcgc gtgaacgtcg
     1021 tggcgcccgg accggtctgg acgccgctca accccgccga ccagcagccg gaaaaggtcg
     1081 ccaaattcgg ctcgcagacg gtgatgaaac gcccggccca gccggaggaa atcgcccccg
     1141 ccttcgtctt cctcgcctca ccccagatgt cgagctacat caccggcgaa atcctgccga
     1201 tcatcggcgg ctatggcggc ggctgatcct acgccaccac gtcgtcgtac tcgcggtgac
     1261 gtttcagcca ggcgacggcg tagctgcagc gcgggctcag cttgagctct tccgcccgcg
     1321 catgctcggc cagcgcctgc atgaactgcc ccgcggcgcc ggagccgcgc aacgccgggt
     1381 ccgcctcgac gtgcaggatg atccgcgtcg agccttgcgt cgcatagtcg gcgaaaaccg
     1441 tccgcagctg gccttcggga tccgtaaagc cctgctcgaa ccgctgctcg tctcgcacgt
     1501 cccggaactc ggtcatctcg ccactctctg tctgacagcc gacacaaggc caacacggcg
     1561 acccgcgcct gcgttcccga aagatcaggg aaatacgtgt tttcggcgac tcgtgaccgc
     1621 agcgcctcgg tctagggcgc cttgtccccg accagattga tcaggccgat gatgctcttg
     1681 atcgtgaacc agacggtcag gaacagcagg cccaggccca gcacgatggc ggccgggacc
     1741 agcaccagac ccagcatcgg cgccgcggcg atgctggcga cacagacgcc gatcaggatc
     1801 cacaggccga tcgt
//
LOCUS       DASVVN010000286         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_35373, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000286 DASVVN010000000
VERSION     DASVVN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35373"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VF686_04550"
     CDS             complement(<1..654)
                     /locus_tag="VF686_04550"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8661115.1"
                     /translation="MQFRTAGVIGAGAWGTALAQVAGWAGLEVLLQAREPEVVESIRE
                     RRVNEAFLPGITLDDHVSVTAELADLAACDVILAVPPAQHMRSTLTAFAAHHRPGVPV
                     ILCSKGIERGSLKLMTDVLAETLPAAPAAVLSGPSFAGEVARGLPSAVTLACADEALG
                     EELMWTLSAPGFRPYLASDLIGAEVGGAIKNVLAIACGMAEGRGLGRSAHAALITRGF
                     "
     gene            complement(668..1861)
                     /gene="tsaD"
                     /locus_tag="VF686_04555"
     CDS             complement(668..1861)
                     /gene="tsaD"
                     /locus_tag="VF686_04555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX8661116.1"
                     /translation="MRVVAPGERGRYLDPMDRPADYSSEGSRATEARRGIPSGGLTVL
                     GIETSCDETAASVVRLTADGEATVLSSVVHSQIDDHAAFGGVVPEIAARSHVEMIDGV
                     VRRAMDEASLSYDALDGVAATAGPGLVGGVMVGLSFGKAVALARDLPLIAVNHLEGHA
                     VSARLGAMVDYPFLLLLVSGGHCQLLEVRGVGDVSRLGTTIDDAAGEAFDKIAKALGL
                     GYPGGPALEKLAASGDGARFNLPRALLGRKDCDFSFSGLKTAASRLAQSCETDQDRAD
                     LADAVQSAIARQLSERSDRALKAYAERHPHRLFVVAGGVAANKTVRATLEATAAANGF
                     GFLAPPMAYCTDNAAMIALAGAERLQKGLTSDIDTAARPRWPLDEQSALADPVHPPGR
                     KGAKA"
     gene            1860..>2133
                     /locus_tag="VF686_04560"
     CDS             1860..>2133
                     /locus_tag="VF686_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8661117.1"
                     /translation="MAHLVRIGTRRSKLAMTQSGMMQRAIGRALGVADADLAEAVPLV
                     EIVTTGDRIQDRRLMEIGGKALFTKEIEEALIAGRVDVAVHSMKDVP"
BASE COUNT          316 a          714 c          809 g          294 t
ORIGIN      
        1 gaagccgcgg gtgatcaggg cggcatgggc gctgcggccc agaccgcgcc cctcggccat
       61 gccgcaggcg atggccagaa cgttcttgat cgcgccgccg acctcggccc cgatcaggtc
      121 ggaggccaga tagggacgga agccgggggc ggagagggtc cacatcagtt cctcaccaag
      181 cgcctcgtcg gcgcaggcca gggtgacggc gctgggcagg ccgcgcgcga cctcgcccgc
      241 gaaactggga ccggacagga cggcggcggg ggcggcgggc aaggtctcgg ccaggacgtc
      301 ggtcatcagc ttgagcgagc cgcgctcgat gcccttggag cacaggatga cgggcacgcc
      361 cggcctgtgg tgcgcggcaa aggccgtcag ggtggagcgc atgtgctggg ccggcggcac
      421 ggcgaggatg acgtcacagg ccgcgaggtc cgccagctcg gcggtcaccg agacgtggtc
      481 atcgagggtg atcccgggta gaaaagcctc gttgacgcgg cgctcgcgga tgctttcgac
      541 gacctcgggt tcacgcgcct gaagcaggac ctcaaggccc gcccagccag ccacctgggc
      601 cagggcggtg ccccaggcgc cggcgccgat cactcccgcg gtcctgaact gcatgcgtat
      661 ctgtccctta ggccttggcg cccttgcggc ccggcggatg gaccgggtcg gcgagcgcgc
      721 tctgttcgtc cagcggccag cgcggccggg cggcggtatc gatgtcggag gtcaggccct
      781 tctgcaagcg ctctgcgccg gcgagggcga tcatcgccgc attgtcggtg caataggcca
      841 tgggcggtgc gaggaagccg aagccgttgg cggcggcggt cgcctccagc gtggcgcgga
      901 cggtcttgtt cgccgcgaca ccgcctgcga ccacaaacag ccggtgggga tggcgttcgg
      961 cataggcctt gagggcgcgg tcggagcgtt cgctgagctg gcgggcgatg gcgctctgga
     1021 cagcgtcggc gaggtccgcg cggtcctgat cggtctcgca ggactgggcc aggcgcgagg
     1081 cggcggtctt gaggcccgag aaggagaagt cgcagtcctt gcggcccagc agcgcccgcg
     1141 gaaggttgaa ccgggcgccg tcgccggacg cggccagttt ctccaaggcc gggccgcccg
     1201 ggtagccgag gcccagcgcc ttggcgatct tgtcgaaggc ctcgccggcc gcgtcgtcga
     1261 tggtggtgcc cagccggctc acgtcgccga cgccgcgcac ctcaagcagc tggcagtggc
     1321 cgcccgagac cagcagcagc aggaagggat agtcgaccat ggcccccaga cgggccgaga
     1381 cggcgtgacc ctcgaggtgg ttgacggcga tcagcggcag atcgcgggcc agggccacgg
     1441 ccttgccgaa gctgaggccg accatgacgc cgccgactag tccggggccg gcggtggcgg
     1501 cgacgccgtc gagcgcgtca taagaaaggc tggcctcgtc catggcgcgg cggacgacgc
     1561 cgtcgatcat ctcgacgtgg ctgcgtgcgg cgatttcggg aacgacgccg ccgaaggccg
     1621 cgtgatcgtc gatctggctg tgcaccacgg acgacagtac ggtcgcctcg ccgtccgcag
     1681 tcaggcgcac gaccgaggcg gcggtctcgt cgcagctggt ctcgatgccc aggacggtca
     1741 ggccgccgga cgggatgccg cggcgggcct cggttgcccg gctcccttcg ctgctatagt
     1801 cggcgggcct gtccatcggg tcgaggtagc gaccccgttc acccggcgca acgactctca
     1861 tggctcacct cgtccgcatc ggcacgcgcc gctcgaaact ggcgatgacc cagtcgggca
     1921 tgatgcagcg cgccatcggc cgggccctcg gcgtcgccga cgccgatctg gcggaggccg
     1981 tgccgctggt cgagatcgtc accacgggcg accgcatcca ggaccggcgg ctgatggaga
     2041 tcggcggcaa ggcgctgttc accaaggaga tcgaggaggc tctgatcgcc gggcgcgtcg
     2101 acgtcgccgt ccattcgatg aaggacgtgc ccg
//
LOCUS       DASVVN010000287         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_63032, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000287 DASVVN010000000
VERSION     DASVVN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63032"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..625
                     /locus_tag="VF686_04565"
     CDS             23..625
                     /locus_tag="VF686_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661118.1"
                     /translation="MRIAVVGLALIVGLYATMIVMVLIGGAVGFAVWGAGSPVNWLVI
                     TGLVVTGFVAVFVALARVSLIMPASVLYRTFAFAEGWRLGREQTGRLFGMMLLLLLLL
                     MVVQFVVYGIGIAIVVGAGISGGFDWASLQSENMDANPFDGLTAMFAANWPWFAVGAL
                     VVAMIYGALVTLHVGPYASACRQLAGSETPSPVDEPASVA"
     gene            656..1576
                     /locus_tag="VF686_04570"
     CDS             656..1576
                     /locus_tag="VF686_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661119.1"
                     /translation="MAFSATDAAFEGFRLVRRNPLALVAWTLLYAVLTLTALFSLSTM
                     VGSLEAWSVQAEAMESVDQPSLEQVMALMSGFGAIMAQVAWLLPVSLVVGAMLGAAVS
                     RGVLNPSSDRFGYLRLGMDEVRVLVVTFVLTLIMGFAAVIAFIALGVMIAAGKAAGDG
                     PAAIAGLIGVLAILCGFVWLSVRLSLAVPITVAEKRFAFFDSFALTKGRFWPLLGMAV
                     LAIVMVLLVEFLSSIVTLPLGMISGLESWSLNADQDSEAVVAALDVTNPWVIAHALVE
                     AVVAALTVGILYAPFAAAYRDIRGAATPTD"
     gene            complement(1573..>2596)
                     /locus_tag="VF686_04575"
     CDS             complement(1573..>2596)
                     /locus_tag="VF686_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX8661120.1"
                     /translation="PAGLFTCVTGVSGGGKSTFTIETLYKAAARRLHNASDAPAPFDR
                     IEGLEMFDKVIDIDQSPIGRTPRSNPATYTGAVQPIRDWFAALPEAKARGYGPGRFSF
                     NVKGGRCEACQGDGLIKIEMHFLPDVYVTCDVCKGKRYNRETLEIVFKGKSISDVLDM
                     TVEEAASFFKAVPSIRDKMLTLNRVGLGYVKVGQSATTLSGGEAQRVKLSKELSKRAT
                     GKTLYILDEPTTGLHFEDTRKLLEVLQELVENGNTIVVIEHNLDVIKVADWLLDFGPE
                     GGDGGGEIVAVGTPEQVAANPASWTGKYLKEVLDRHEERRKARVAALSGRAAADDKPA
                     RAKKRA"
BASE COUNT          331 a          832 c          903 g          530 t
ORIGIN      
        1 gctgcgtctc ggcatggacg agttgaggat cgcggtcgtc ggactggcgc tgatcgtggg
       61 cctgtacgcg accatgatcg tcatggtcct catcggaggc gcggtcggct tcgcggtgtg
      121 gggcgcaggc tcgccggtga actggctggt cataaccggt ctcgtcgtca ccggttttgt
      181 ggcggttttc gtcgcattgg cccgggtcag cctgatcatg cccgccagcg tgctgtatcg
      241 taccttcgca ttcgccgaag gctggcggct ggggcgggaa cagacgggcc gcctctttgg
      301 catgatgctc ctgctcctgc ttctgctcat ggtggtccag ttcgtcgtgt acggcatcgg
      361 catcgccatc gtcgttggcg ccgggatttc gggcggcttc gactgggcgt cgcttcagtc
      421 ggagaacatg gacgccaacc cgttcgacgg cctgaccgcc atgtttgcgg cgaactggcc
      481 gtggtttgct gtgggagcgc ttgtggttgc catgatctat ggagccttgg tgaccctgca
      541 cgtcggtccc tacgcttcgg cgtgccggca actggccgga agcgaaacgc cgtcccccgt
      601 ggacgagccc gcgtcggtcg cctaagccag catcggtcag agacaggaga ccaccatggc
      661 gttttcggcc accgacgcgg ccttcgaggg ctttcgcctc gtgcggcgca atccgctggc
      721 gctggtcgcc tggacgctgc tgtacgccgt cctgacgctg acggccctgt tctccctgtc
      781 caccatggtg ggctcgctgg aggcgtggag cgtccaggcc gaggcgatgg agtccgtgga
      841 ccagcccagc ctcgaacagg tcatggccct gatgagcggc ttcggcgcga tcatggccca
      901 ggtggcctgg ctgcttccgg tcagtctggt cgtcggcgcc atgctcgggg cggcggtgtc
      961 gcgcggcgtg ctgaatccgt ccagcgaccg gttcggctat ttgcgcctcg gcatggacga
     1021 ggtgcgggtg ctggtggtga ccttcgtgct gaccctgatc atgggcttcg ccgccgtgat
     1081 cgccttcata gccctcggcg tgatgatcgc ggccggcaag gccgcggggg acgggccggc
     1141 ggccatcgcc ggtctgatcg gcgttctggc catcctctgc ggcttcgtct ggctgtccgt
     1201 ccggctgagc ctcgcggtgc cgatcaccgt ggccgagaaa cgcttcgcct tcttcgactc
     1261 cttcgccctg accaagggcc gtttctggcc gctgctgggc atggcggttc tggccatcgt
     1321 gatggtgctg ctggtcgaat tcctgtcgtc gatcgtcacc ctgccgctgg gcatgatctc
     1381 gggtctcgag agctggtcgc tcaacgccga tcaggattca gaggccgtcg tcgccgccct
     1441 cgacgtcacc aatccgtggg tgatcgccca cgccctggtc gaggcggtcg tggccgcact
     1501 gactgtcggg atcctgtatg cgcccttcgc cgccgcctat cgcgatataa ggggcgcggc
     1561 gacgccgacc gactaggccc gcttcttcgc ccgcgccggc ttgtcgtcgg cggcagcgcg
     1621 gccggacagg gccgccacgc gcgccttgcg acgttcttca tggcggtcga ggacctcctt
     1681 caggtatttg ccggtccagc tcgcggggtt ggcggcgacc tgttcggggg tgccgaccgc
     1741 gacgatctcg ccgccgccgt cgccgccttc gggaccgaag tccagcagcc agtcggcgac
     1801 cttgatgacg tcgagattgt gctcgatgac cacgatggtg ttgccgttct cgaccagctc
     1861 ctgaagcacc tccagcagct tcctcgtgtc ctcgaaatgc aggccggtcg tcggctcgtc
     1921 gaggatgtag agggtcttgc cggtggcgcg tttcgacagc tctttcgaca gcttgacccg
     1981 ctgggcctcg ccgcctgaga gggtcgtcgc cgattgcccg accttgacgt agccgagccc
     2041 gacccggttg agggtcagca tcttgtcgcg gatcgagggt acggccttga agaagctcgc
     2101 cgcttcctcg accgtcatgt ccagaacgtc ggatatgctc ttgcccttga agacgatttc
     2161 cagcgtctcg cggttgtagc gtttgccctt gcagacgtcg caggtgacgt agacgtcggg
     2221 gaggaaatgc atctcgatct tgatcaggcc gtcgccctga caggcctcgc agcgcccgcc
     2281 cttcacgttg aacgagaacc gtcccgggcc atagccgcgc gccttggcct ccggcagcgc
     2341 ggcgaaccag tcgcggatcg gctggacggc gccggtgtag gtcgccgggt ttgagcgggg
     2401 cgtgcggccg atgggcgact ggtcgatgtc gatgaccttg tcgaacatct ccagcccctc
     2461 gatgcggtcg aagggggcgg gggcgtccga ggcgttgtgc agccggcggg cggcggcctt
     2521 gtagagggtc tcgatcgtga aggtcgactt gccgccgccg gacacgccgg tgacgcaggt
     2581 gaacaggccg gcgggg
//
LOCUS       DASVVN010000288         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_510294,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000288 DASVVN010000000
VERSION     DASVVN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510294"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VF686_04580"
     CDS             <1..1489
                     /locus_tag="VF686_04580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8661121.1"
                     /translation="DENTWTVIPSEAGVAGLPASNKAAAAAAARSRNVQGWAIVNTRS
                     SVDPFLSFADDRNLREVVWRKFVNRGDNGDRNDTNATIAEIVKLRQERARLLGFGNHA
                     EWRMQDTMAKTPRAAMDLMNRVWAPAKARVAEEVADMRAIAGHDIEPWDYLYYAEKVR
                     KAKYDLDQNELKPYFELNNVRAGSFEMARRLYGFTFDKLPAGAVPVFHPDVTVYEVKD
                     AGRHIGLYYADDYSRAGKRSGAWMTTYRSHSTYDGEKNTLGSNNNNFVKAEGGEPILI
                     SLDDAETLFHEFGHTLHAYSSKVTYPALGGTPRDYVEYPSQVHEHWVLSRPILDGYMK
                     HYQTGEPMPQALVDKVQAAQTFNQGYATVSYLSSALVDMDLHTQTTPPTDIDAFERES
                     LARYGMPKEIVMRHRLPQFNHLFTSDAYSAGYYSYLWSEVMDADTWAYFEESGDVFNP
                     DIAARYKAIILAEGNSTDRAEAYRRFRGRDPDVTALLKVRGFPTS"
     gene            complement(1598..1912)
                     /locus_tag="VF686_04585"
     CDS             complement(1598..1912)
                     /locus_tag="VF686_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX8661122.1"
                     /translation="MIKTALALAAATLLYAAPAAAQTVRVPFGDLNLSTAAGAEAFDA
                     RAAAAARAACAVSPRLVNDNCVRRVQQEAVRELPAARQDDYVRARRGDRILAMVAPAW
                     PV"
     gene            complement(2086..2385)
                     /locus_tag="VF686_04590"
     CDS             complement(2086..2385)
                     /locus_tag="VF686_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX8661123.1"
                     /translation="MKALASLAAIAALAVAGSASAQEARIAWGDLDMSSAAGANAFDA
                     RIEAAARSLCRGVKKPGSGVSDRAFCRAAVRAEAVRLLPGPVQVDYALSRLPLVV"
BASE COUNT          436 a          814 c          815 g          359 t
ORIGIN      
        1 cgacgaaaac acatggaccg tcatcccgag cgaagcgggc gtcgccggcc tgcccgcctc
       61 gaacaaggcc gccgcagccg ccgccgcgcg cagccgcaac gtccagggct gggccatcgt
      121 caacacccgc tccagcgtcg atcccttcct cagcttcgcc gacgaccgca atctgcgcga
      181 agtggtctgg cgcaagttcg tcaaccgcgg cgacaacggc gaccgcaacg acaccaatgc
      241 gaccatcgcc gagatcgtga aactgcgtca ggagcgcgcc cgcctgctgg gcttcggcaa
      301 ccacgccgaa tggcggatgc aggacaccat ggccaagacg ccccgtgcgg ccatggatct
      361 gatgaaccgc gtctgggccc cggccaaggc gcgcgtcgcc gaggaggtgg ccgacatgcg
      421 cgcgatcgcc ggccacgaca tcgagccgtg ggactatctg tactatgccg agaaggtccg
      481 gaaggcgaag tacgacctcg atcagaacga gctgaaaccc tatttcgaac tcaacaacgt
      541 ccgcgccggc tcgttcgaga tggcgcgccg cctgtacggc ttcaccttcg acaaactgcc
      601 cgccggcgcc gtgccggtct tccacccgga cgtgaccgtc tatgaggtca aggatgcggg
      661 ccgccacatc ggcctgtact acgccgacga ctattcgcgc gccggcaagc ggtcgggggc
      721 gtggatgacg acctaccggt cgcattcgac ctatgacggc gagaagaaca ccctcggttc
      781 gaacaacaac aatttcgtca aggcggaggg cggcgagccg atcctgatct cgctggacga
      841 cgccgagacc ctgttccacg agttcggcca caccctgcac gcctattcgt cgaaggtgac
      901 ctatccggcc ctgggcggca cgccgcgcga ctacgtcgag tatccgtcgc aagtgcacga
      961 gcactgggtg ctgagccggc cgatcctcga cggctatatg aaacactacc agaccggcga
     1021 gcccatgccg caggcgctgg tcgacaaggt gcaggcggcc cagaccttca atcagggcta
     1081 tgcgaccgtc agctatctgt cgtcggccct ggtcgacatg gacctgcata cgcagaccac
     1141 gccgccgacc gacattgacg ccttcgagcg cgagagcctg gcgcgctacg gcatgccgaa
     1201 ggagatcgtg atgcggcacc gcctgccgca gttcaatcac ctgttcacct cggacgccta
     1261 ttcggccggc tattacagct acctctggtc agaggtgatg gacgctgaca cctgggccta
     1321 tttcgaggag tcgggcgacg tcttcaatcc ggacatcgcc gctcgctaca aggccatcat
     1381 cctggccgag ggcaacagca ccgaccgcgc cgaggcctat cgccgcttcc gcggccgcga
     1441 cccggacgtg acggccctgc tcaaggtccg gggcttcccg acttcctagg agccctaccg
     1501 cccttcgggc tgcttgaggg cgccgcgcgg agcgcggatg tgtgaaagag gagaggccgg
     1561 tggaggcatc caccggcctc ttcgttttcc gctgacctca caccggccag gcgggggcca
     1621 ccatggcgag gatgcggtcg ccgcggcggg cgcggacgta gtcgtcctga cgcgcggccg
     1681 gaagttcgcg cacggcttcc tgctgcacgc gtcgcacgca gttgtcgttg acgaggcgcg
     1741 ggctgacggc gcacgccgca cgcgccgcgg cggcggcgcg tgcgtcgaag gcttcggcgc
     1801 cagctgcggt cgacaggttg aggtcgccga acggtacgcg gacggtctgg gccgccgccg
     1861 gggctgcata gagcagggtc gcggcggcga gggcgagggc ggtcttgatc atctgaaatc
     1921 ctcctgtcgc ggggggagtg cgacgacaag gtagatgata ggcttgcgcg acagtgacca
     1981 gaccagaagt gcgacaatta ctggtttaca ggccgagaac tgtcaaggct gacaaaagtt
     2041 tgacttatgt cagtccgtgc tgcccgaagc ggcgccgccc tgaagtcaga ccaccagggg
     2101 aaggcggctc agggcgtagt cgacctgcac cggccccggc agcagccgca cggcctcggc
     2161 ccgcacggcg gcgcggcaga aggcgcggtc gctgacgccg cttccgggtt tcttgacgcc
     2221 ccggcacagg gaccgggcgg cggcctcgat gcgggcgtcg aaggcgttgg cgccggcggc
     2281 gctggacatg tccagatcac cccaagcgat gcgcgcctcc tgggccgagg cggagccggc
     2341 gacggcgagg gccgcgatgg cggcgagaga agcgagagct ttcatgacga actcctgggg
     2401 agatcaacgt cgctcttggc ggcg
//
LOCUS       DASVVN010000289         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_381110,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000289 DASVVN010000000
VERSION     DASVVN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381110"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF686_04595"
     CDS             <1..341
                     /locus_tag="VF686_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8661124.1"
                     /translation="ELFGCTFDTAANGREAIRQVEAMPFDLVLMDIKMPVMDGVEATR
                     AIRALPGPVGRIPILALTANADPRDEIEYLAAGMNGVSQKPIEPDALLNAIRMVFSQI
                     ATRVPVAEAA"
     gene            375..2387
                     /locus_tag="VF686_04600"
     CDS             375..2387
                     /locus_tag="VF686_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661125.1"
                     /translation="MQIPPPPQLPDVVVTSARLPPSEGEAAFSVVRLGADDLRVQQRL
                     DEALATVPAVSLFRRNSSLAANPTTQGISLRAIAPSGAGRTLVTLDGVPLNDPFGGWV
                     IWSQVPTESLSGLDIVRGAGAGPYGAGALTGVIALRERDAGSVLDASIAEQGGARLAG
                     SGATALGPVRLVASGLYETSDGYVPVRGAAAGAADTPLDLEIRSAALRADVPVAVAVV
                     SLRAAAFEEDRGSGLAGARATASGNLLSATAAVQPRADRPGWRLQAWRRESDLANTSV
                     AVAADRATTTPANDQYETPATGWGVNAALRRVTVSGIARVEWELGADARFNEGETRER
                     FRYMAGAFTRDRVAGGETSVAGVYAEGAWTGGPWLVAGGLRYDGWRNENGRRLERDLA
                     SGLPTLDETDPDRSGEVVSARLAARRDLGGGQALRLAAYTGFRPATLNELHRPFRVGN
                     DLTEANAALEPERLTGVETGWAWTGDRASLTATAFWNRIEDAVVNVTIGQGPATFPRA
                     GFVPAGGVLRQRRNAGTIEATGVELEGLFELTDAVSLRAALSATDARVDGGAQAPQLT
                     GLRPAQAPIWSAVAGVDWRPVDRLSLAADLRWESRRFEDDLNSRVLDAAGTADLRAEW
                     EMTPSANLWLAADNLFDAEVETGETGTGVASFGPPRTVSIGLRLTR"
     gene            complement(2391..2900)
                     /locus_tag="VF686_04605"
     CDS             complement(2391..2900)
                     /locus_tag="VF686_04605"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX8661126.1"
                     /translation="MTHPASRPFPDPTELLPGADFGDRYALTVDGQDLDAAEGSRRMF
                     GHAPPWIRGLLGLRNLITRPFGLKPGRNRTAPATPQIGIFPVIRQSPGEVVMGLNDRH
                     LDFRVSVEIRNLDAGRQDISVSTAVRTHNLFGRGYLTVVKPFHRLIVPSMLKQAAFAP
                     NRSREIAEP"
     gene            3038..3688
                     /locus_tag="VF686_04610"
     CDS             3038..3688
                     /locus_tag="VF686_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX8661127.1"
                     /translation="MTEAAAPAKPKPALNAKRSFWLKQLHQWHWISAAMSLIGLLLFA
                     ITGITLNHAAQIPATPATVEQTGQLPAPLLARLADLPEETTEPVPPAVARWASETFKV
                     RVAGRATETTPDEVYISLPEPGGDGWLTIDRATGAALRERTTRGAIAWLNDLHKGRNT
                     GPVWYWFIDVFAAACIVFAVTGFGLTWLHARQRPSTWPLLGLGLLIPAVIALLFIH"
     gene            3696..4202
                     /locus_tag="VF686_04615"
     CDS             3696..4202
                     /locus_tag="VF686_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEX8661128.1"
                     /translation="MRQLPLILTATAAAAVAGPALAADITVSVEVPRLSVAAYHRPYV
                     AVWIERPDNTPVRTLAVWYEVSNVAEGKDWLKDMRTWWRRGGRSLSLPMDGISSATKA
                     PGRHVVTVPAARLRDLPAGNYVMVVEAARELGGRESVRVPFRWGAANTARASGSTELG
                     AVSVAVSR"
BASE COUNT          615 a         1602 c         1414 g          627 t
ORIGIN      
        1 tggagctgtt cggctgcacc ttcgacaccg ccgcgaacgg ccgcgaggcg atccggcagg
       61 tcgaggcgat gccgttcgat ctggtgctga tggacatcaa gatgccggtc atggacggcg
      121 tggaggcgac ccgggcgatc cgggccctgc cgggtccggt cggacgcatc cccatcctgg
      181 ccctgaccgc caacgccgat ccccgggacg agatcgaata tctggccgcc ggcatgaacg
      241 gggtgtcgca aaagccgatc gagcccgacg ccctcctcaa tgcgatccgg atggtgttca
      301 gccagatcgc aacgcgggtc ccggtagccg aagccgcttg aacccggcga ggccgtctgg
      361 cctaggtggg gtgtatgcag attccgccgc ccccccagct tcccgatgtc gtcgtcacaa
      421 gcgcgcggct cccaccctcc gagggcgagg cggcgttttc cgtcgttcgt ctgggtgcgg
      481 acgatctgcg tgtgcagcag cgactggacg aggcgctggc aaccgtgccc gccgtatccc
      541 tgttccggcg caactccagc cttgcggcca atccgaccac ccagggcata tccctgcgcg
      601 ccatcgcccc ctcgggcgcg ggccgcaccc tcgtcaccct cgacggcgtg ccgctgaacg
      661 atccgttcgg cggctgggtc atctggtccc aggtcccgac cgaaagcctg tccggcctcg
      721 acatcgtcag gggcgccggc gcgggtccgt acggcgcggg agccctgacc ggcgtgatcg
      781 ccctgcgcga acgcgacgcc ggttcggtgc tggacgcctc tatcgctgaa cagggtggag
      841 cccgcctcgc cggttcgggc gcgaccgcgc tcggccccgt ccgtctcgtc gcatccggcc
      901 tgtacgagac cagcgacggc tatgtccccg tacgcggagc cgccgcaggc gccgccgaca
      961 cgccgctgga cctcgagatt cgcagcgcgg ccctgcgcgc cgatgtgccc gtggccgttg
     1021 cggtcgtctc cctgcgcgcc gccgccttcg aggaggaccg cggttccggt ctcgccggag
     1081 cgcgggcgac cgccagcgga aacctgctct ccgccacggc cgccgtgcag ccccgcgccg
     1141 accgtcccgg ctggcggctt caggcctggc ggcgcgagag cgatctggcc aacacctccg
     1201 tcgccgtcgc cgccgaccgc gccacgacga cgcccgccaa cgaccagtat gagaccccgg
     1261 ccacgggctg gggcgtcaac gccgccctgc gccgggtcac tgtcagcggt atcgcccggg
     1321 tcgaatggga actgggcgcc gacgcccgct tcaacgaggg cgagacccgt gagcggttcc
     1381 gctacatggc cggcgccttc acccgcgacc gcgttgccgg gggcgagacc tcggtcgccg
     1441 gcgtctatgc cgaaggcgcc tggaccgggg ggccgtggct ggtggccggg ggcctgcgct
     1501 acgacggctg gcgcaatgag aacgggcgcc ggctggagcg cgatctcgcc agcggcctgc
     1561 cgacgctgga tgagaccgat cccgaccgct cgggcgaagt ggtcagcgcc cgcctcgccg
     1621 cgcgccgcga cctgggcggc ggacaggccc tgcgcctcgc cgcctacacc ggcttccggc
     1681 ccgccaccct gaatgagctg catcgcccct tccgcgtcgg caacgacctg accgaggcca
     1741 atgccgcgct ggagcccgaa cggctgaccg gcgtcgaaac gggctgggcc tggaccggcg
     1801 accgcgcctc cctgaccgcc acggccttct ggaaccggat cgaggacgcc gtggtcaacg
     1861 tcaccatcgg ccaaggcccc gcgaccttcc cgcgcgccgg cttcgtgccc gcgggtggcg
     1921 tgctgcgcca gcggcggaac gccggaacca tcgaggcaac gggggtggaa ctggaaggcc
     1981 tgttcgaact gaccgacgct gtgtccctgc gcgccgccct gtcggcgacc gacgcccgcg
     2041 tcgacggcgg agcccaggcg ccgcaactga ccggcttgcg tccggcgcag gcgccgatct
     2101 ggagcgccgt cgccggcgtc gactggcggc cggtcgatcg cctcagcctg gcggcggacc
     2161 tgcgctggga gagccgccgg ttcgaggatg acctgaacag ccgcgtcctc gacgccgccg
     2221 ggacggccga cctgcgcgcc gaatgggaga tgaccccatc cgccaacctg tggctcgccg
     2281 ccgacaatct gttcgacgcc gaggtcgaga ccggcgaaac cgggaccggc gtcgccagct
     2341 tcggcccacc ccgcaccgtc agcatcggct tgaggctgac gcgctgaagg ttacggctcc
     2401 gcgatctcgc gcgagcggtt cggagcgaac gcagcctgct tgagcatcga cggaacgatg
     2461 aggcggtgaa acggcttcac gaccgtcaga tagccgcgac cgaacaggtt gtgtgtcctg
     2521 accgccgtcg aaaccgagat gtcctgccgg ccggcgtcga ggtttcggat ctcgaccgag
     2581 actcggaaat cgagatgccg gtcattcagg cccatgacca cctcgccggg cgactggcgg
     2641 atgaccggga atattccgat ctgcggcgtg gcgggcgcgg tccggttccg tccgggtttc
     2701 agaccgaacg gcctcgttat caggttccgc aaccccagca gtcctctgat ccagggcggc
     2761 gcatggccga acatgcgccg ggacccttcc gcggcgtcca gatcctgccc gtccaccgtc
     2821 agcgcatatc ggtccccgaa gtccgcgccg ggcagcaact ccgtcggatc aggaaacggt
     2881 cgggatgcgg ggtgggtcat ggcggccacc gtcctcccgg ctgcaggcgc ggtcaacgca
     2941 tccggggtct ggcagactgc ggcatatggg agcgacgccg gctgttagcc cgacgggctc
     3001 tcttgcaaac ggttctcaac ttcggcacaa gccggacgtg accgaagccg ccgcccccgc
     3061 caagcccaag ccggccctga acgccaaacg gtcgttctgg ctgaagcaac tgcaccagtg
     3121 gcactggatc tcggcggcta tgagcctgat cggcctgctg ctgttcgcga taacggggat
     3181 caccctcaac cacgctgcac aaattccggc aaccccggcc acggtcgaac agaccggtca
     3241 actgcccgca cccctgctgg cgcgtctggc cgacctgccc gaggaaacca ccgagccggt
     3301 cccgcccgcc gtcgcgcgat gggcttcgga aaccttcaag gttcgggtcg ccggtcgcgc
     3361 caccgaaacc acccccgacg aggtctatat ctccctgccc gagcccggcg gcgacggctg
     3421 gctgaccatc gaccgcgcca ccggcgccgc cctgcgcgaa cgcaccacgc gcggcgcaat
     3481 cgcctggctg aacgacctgc acaagggccg caacaccgga ccggtctggt actggttcat
     3541 cgatgtcttc gccgccgcgt gcatcgtctt cgccgtcacc ggcttcggtc tgacctggct
     3601 gcacgcccgt cagcgtccct cgacctggcc gctgcttggc cttggtcttc tgatacccgc
     3661 cgtcatcgcc cttctgttca ttcactgagt tcccgatgcg ccagcttccc ctcatcctga
     3721 ccgccaccgc cgcggccgcc gtcgccgggc cggccctggc cgccgacatc acggtctccg
     3781 tcgaggtgcc gcgcctgtcg gtcgccgcct atcaccgtcc gtatgtcgcc gtctggatcg
     3841 agcggcccga caacaccccc gtgcgcaccc tcgccgtctg gtacgaggtc tccaacgtcg
     3901 ccgagggcaa ggactggctc aaggacatgc gcacgtggtg gcgccgcggc ggccgctcgc
     3961 tgagcctgcc catggacggg atctccagcg caaccaaggc cccgggccgt cacgtcgtca
     4021 ccgtgcccgc cgcgcgtctc cgtgatctgc ccgcgggcaa ctacgtcatg gtcgtcgagg
     4081 ccgcccgcga actgggcggc cgtgaatccg tgcgcgtgcc cttccgctgg ggcgccgcca
     4141 ataccgcccg cgcgtcgggc tctaccgaac tgggcgcggt cagcgtcgcc gtcagccgct
     4201 aaggaagcac accatgaaga agcgtatcgc cctgctggcc gctctcgccg ccttcgcc
//
LOCUS       DASVVN010000290         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_431443,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000290 DASVVN010000000
VERSION     DASVVN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431443"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            575..1444
                     /locus_tag="VF686_04620"
     CDS             575..1444
                     /locus_tag="VF686_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX8661129.1"
                     /translation="MTLPHALNGLSEIAADYDILLCDVWGVIHNGRESWPAACDALTR
                     FNRDHGQVVLISNSPRPSPGMILQMDGLGVPRSSWGAVVTSGDATRVELARRAPGPAW
                     IIGPDRDAPLYDRLGLKTAQGAGDAAFISVTGMIDDEVETPEDYRDRLSAGAARDLEM
                     ICANPDRVVQRGDKLIYCGGSLADLYESLGGRVTMAGKPYGPIYDLALKEAADLLGRP
                     VDRSRVLCIGDGVVTDVLGANKQALDCLFIAQGIHGDAARGPDGRLDPARAADLLKAE
                     TAYARWAMLDLTW"
     gene            1474..>1715
                     /locus_tag="VF686_04625"
     CDS             1474..>1715
                     /locus_tag="VF686_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8661130.1"
                     /translation="MVELVTKHPSGGYILEELHVGMTAVKPILVTEQRIQQFADASDD
                     FNPVHMDEAFASKTAYRGRIAHGLLSASFGSAVVGT"
BASE COUNT          273 a          634 c          532 g          276 t
ORIGIN      
        1 cttcgaacgc cccggaatga cggtgtggat gctgcaggtt cgcgctgaaa cagacgagtt
       61 gcagaagcca acctgctgac ctttcactgg atttcagaac gaccagcgtc atttcgtagc
      121 cttggttgag acccgtgcca cacttctccc atcccgtcgg tggggagggc gtcggatcca
      181 cgcttctcga tgtcggcggg ctgtggtcga gcgatgaagc ccgggggagg cagacctcgg
      241 gggtccttag gctcagcgat ggatgctggg ccctgctcgc cgcgcgcttc tcgtgcgggg
      301 aatctctggg gctatacgcc ttaatgtccc cctcccggtc cgctccgcaa ggcgcgaacc
      361 gggtgtcggc aaggccagac aacggccggc ggccggcggg cgcgtggaaa acgacgacgc
      421 ggggcggctg tctcgtcgaa acggtaaaac tacaaccagc accgggcgag cccggtcgcc
      481 ccgcaccctc cccgaacttt tccgcggaaa cgctgaagag cgatgacgca tgcctgtact
      541 tctcccctcc cctccgaaag gctatctccg ccccatgacc ctcccccacg ccctgaacgg
      601 cctgtccgag atcgccgcgg actacgacat cctgctctgc gacgtctggg gggtgatcca
      661 taacggccgc gaaagctggc ccgcggcctg cgacgccctg acccgcttca accgcgatca
      721 cggccaggtc gtcctgatct ccaactcccc gcgtccctcg ccgggcatga tcctgcagat
      781 ggacggcctc ggcgtcccgc gcagttcctg gggcgccgtc gtcacctcgg gcgacgcgac
      841 ccgcgtggag ctggcccgcc gcgcgcccgg cccggcgtgg atcatcggac ccgaccgcga
      901 cgcccccctc tacgaccgcc tcggccttaa gacggcgcag ggcgccgggg acgccgcctt
      961 catctctgtc accggcatga tcgacgacga ggtcgagacc cccgaggatt accgcgaccg
     1021 cctgtccgcc ggcgccgccc gcgatctgga gatgatctgc gccaacccgg accgcgtggt
     1081 ccagcggggc gacaagctca tctactgcgg cggttcgctg gccgacctct acgaaagcct
     1141 cggcggtcgc gtcaccatgg ctggcaagcc ctacggcccc atctacgatc tggccctcaa
     1201 ggaagccgcc gacctgctgg gccggcccgt cgatcgttcg cgcgtcctgt gcatcggcga
     1261 cggcgtcgtc accgacgtgc tcggcgccaa caaacaggcg ctggactgcc tgttcatcgc
     1321 ccagggcatc cacggcgacg ccgcccgcgg ccccgacggc cgcctcgatc ccgcccgcgc
     1381 cgccgacctc ctgaaggccg aaaccgctta cgccagatgg gcgatgctcg atctgacgtg
     1441 gtgagcgggg ccgattcagg ctaaggccgg gccatggttg aactcgtcac gaaacacccc
     1501 tccggcggct atatcctcga ggagctccac gtcggcatga ccgccgtgaa gcccatcctg
     1561 gtcaccgaac agcggatcca gcagttcgcc gacgcctcgg acgacttcaa tcccgtccat
     1621 atggacgagg ccttcgcctc gaagaccgcc tatcgcggcc gcatcgccca tggcctgctg
     1681 tcggcctcgt tcggttcggc cgtggtcggg accat
//
LOCUS       DASVVN010000291         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_35831, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000291 DASVVN010000000
VERSION     DASVVN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35831"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF686_04630"
     CDS             <1..231
                     /locus_tag="VF686_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517347.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX8661131.1"
                     /translation="PFTATRYHSLAVRRETLPEVLDVTAWTADGEIMGLAHRTRPIHG
                     VQFHPESIATEHGHDLLANFLDLANVRRRAMV"
     gene            232..1269
                     /gene="trpD"
                     /locus_tag="VF686_04635"
     CDS             232..1269
                     /gene="trpD"
                     /locus_tag="VF686_04635"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX8661132.1"
                     /translation="MADGFKPLLAKLVDGRVLSIDEAHAFFAACLRGEPTPAQVAAAV
                     TALRIRGETVEEIAAFATAMRDAALKLDHPYDVIDTCGTGGDGQHTFNISTAAALVLA
                     GAGLKVAKHGNRALSSKSGSSDVLAALGVNLAATPAQQRRSLDDAGIAFLFAPAYHGA
                     MRHVGPVRAEIGFRTVFNLLGPLSNPASAKRQVMGVYDPRLLEPLAEVLGRLGATRAW
                     TVHGQGLDELTTTGETEVAEWKAGAVRRFTVTPEDAGLPRADISALRGGDAEENALAL
                     QNLLAGEKSAYRDIVLLNAAAALVVADRAADLREGAAMAAAVIDEGRAARALTDLVVA
                     TNAPEPEEDPA"
     gene            1266..>1783
                     /locus_tag="VF686_04640"
     CDS             1266..>1783
                     /locus_tag="VF686_04640"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX8661133.1"
                     /translation="MNDVLTGIAAYKREEVAVRKAATSHDAVEALAQQASAPRGFRAA
                     LERHVEETGRPALIAEIKKASPSKGLIRADFDPPALARAYERGGAQCLSILTDGPSFQ
                     GDDAFLGQARDATALPCLRKDFLVEPWQVAESRALGADCILIILAMVDDTLAAELLTE
                     AGRFGMDALIET"
BASE COUNT          271 a          700 c          570 g          242 t
ORIGIN      
        1 cctttcaccg ccacccgcta tcacagcctc gccgtccgcc gcgaaaccct gcccgaggtg
       61 ctggacgtca ccgcctggac cgccgacggc gagatcatgg ggctggcgca cagaacccgg
      121 ccgatccacg gcgtccagtt ccaccccgag tccatcgcca ccgaacacgg ccacgacctg
      181 ctggccaatt tcctcgatct ggccaacgtc agacgccggg cgatggtctg agtggctgac
      241 ggtttcaaac ccctgctggc caagctggtc gacggccggg tgctgtcgat cgacgaggcg
      301 cacgcctttt tcgccgcctg cctgcgcggc gagcccaccc cggcacaggt cgccgcggcc
      361 gtcaccgccc tgcgcatccg cggcgagacg gtcgaggaga tcgccgcctt cgcgaccgcc
      421 atgcgagatg cggccctgaa actggaccac ccctacgacg tcatcgacac ctgcggcacg
      481 ggcggcgacg gccagcacac cttcaacatc tcgaccgccg cggcgctggt tctggccggc
      541 gcgggcctga aggtcgccaa acacggcaac cgggccctga gttcgaagtc cggctcgtcc
      601 gacgtgctcg cggcccttgg tgtcaacctc gccgccacgc cggcccagca gcgccgctcg
      661 ctggacgacg cgggcatcgc cttcctgttc gcacccgcct accacggcgc catgcgccac
      721 gtcgggccgg tccgggccga gatcggcttc cgcacggttt tcaatcttct agggccgctt
      781 tcaaacccgg cgtctgcgaa acgtcaggtc atgggcgtct atgatccccg cctgctggag
      841 cccctcgccg aggtgctggg ccggctgggc gccacccgcg cctggaccgt gcacggacag
      901 ggcctcgacg aactgaccac cacgggcgag accgaggtgg cggagtggaa ggccggcgcc
      961 gtccgccgct tcaccgtcac gcccgaggac gccggcctgc ctcgcgccga catctccgcc
     1021 ctgcgcggcg gcgatgcgga agagaacgcc ctggccctgc agaaccttct ggcgggcgag
     1081 aagagcgcct accgggacat cgtcctgttg aacgccgccg ccgccctggt cgtcgccgac
     1141 cgcgccgccg atctgaggga aggcgccgcc atggctgccg ctgtcatcga cgagggccga
     1201 gccgcccggg ccctaaccga tctggtcgtc gccaccaacg cgcccgagcc ggaagaagac
     1261 cccgcgtgaa cgacgtcctc accggcatcg ccgcctacaa gcgcgaagag gtggccgtcc
     1321 gcaaggcggc gacctcccat gacgccgtcg aggcgctggc gcagcaggcc tcggccccgc
     1381 gcggcttccg cgccgccctc gagcgccacg tcgaggaaac cggccgcccc gccctgatcg
     1441 ccgagatcaa gaaggccagc ccgtccaagg gcctgatccg cgccgacttc gacccgccgg
     1501 ccctcgcccg cgcctatgag cgcggcggcg cccagtgtct gtcgatcctg accgacggcc
     1561 ccagcttcca gggcgacgac gccttcctcg gccaagcgcg cgacgcgacg gccctgccct
     1621 gcctgcgcaa ggattttctc gtcgagccct ggcaggtggc cgagagccgg gccctgggcg
     1681 ccgactgcat cctgatcatc ctcgccatgg tcgacgacac gctggccgcc gaattgctga
     1741 ccgaggccgg acggttcggc atggacgccc tgatcgaaac cca
//
LOCUS       DASVVN010000292         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_458683,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000292 DASVVN010000000
VERSION     DASVVN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458683"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VF686_04645"
     CDS             complement(<1..871)
                     /locus_tag="VF686_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8661134.1"
                     /translation="MSLALAPAAHAEVIRRNVPAQSLTAARIARLPEPERDAWAAYLD
                     RSHAQALLDRAALALELPAGAVVPPPPMAVGGTQGNMPLDREDAWYATPEARAVADAI
                     VSFQTPAGGWSKNQNRQIARLRGQRFSNDAETMELNPANFDAPADRFWTFVGTLDNGA
                     TTTEMRFLAKVAAHAPGAEGDAWRASITKGVNYLLNAQYPNGGWPQIWPVEGGFHDAV
                     TFNDNAVAEAAMLLEDVVENQNGFAFVSEDLEARAAEAVRDAIEVILAAQVRVDGQLT
                     AWPQQVDPLTLAPS"
     gene            1031..1345
                     /locus_tag="VF686_04650"
     CDS             1031..1345
                     /locus_tag="VF686_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX8661135.1"
                     /translation="MEKIAFCMQLNPGAEAEYRARHDALWPDLRDALRLAGVRDYSIH
                     LEPGTGRLFATLWRSDDHQMDALPDLPVMKRWWRHMADLMSTDSSGAPLQTPLIEMFH
                     LE"
     gene            1514..>2079
                     /locus_tag="VF686_04655"
     CDS             1514..>2079
                     /locus_tag="VF686_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEX8661136.1"
                     /translation="MPANPFLGVIFHWLGGLAAASFYVPYRGVKRWSWEIYWITGGVF
                     SWVVAPWFFASIQTHDLFGVLSATPSSTIGYCILFGGLWGFGGLTYGLTMRYLGLSLG
                     MAVVLGLCTVFGTLIPPIFQGTFVSTLIDSPSGNIVLLGLLVTLAGIIVVALAGAKKG
                     EPVDNAAIAEFDFKKGIGVAVFSGVMSS"
BASE COUNT          313 a          686 c          688 g          392 t
ORIGIN      
        1 tggagggggc cagcgtcagc ggatcgacct gctgaggcca ggccgtcaac tggccgtcga
       61 cccggacctg cgcggcgagg atgacctcga tcgcgtcgcg aacggcctct gccgcgcgcg
      121 cctccagatc ttcggacaca aaggcgaagc cattctggtt ctctaccacg tcctccagca
      181 gcatggctgc ttcggccacg gcgttgtcgt tgaaggtgac ggcgtcgtgg aacccgccct
      241 ccaccggcca gatctgcggc cagccgccgt tcggatactg ggcgttgaga aggtagttca
      301 cacccttggt gatgctggcg cgccaggcgt cgccctcggc gccgggggcg tgggcggcca
      361 ccttggcgag gaagcgcatc tccgtcgtgg ttgcgccgtt gtccagcgta ccgacgaagg
      421 tccagaaacg atcggccggc gcgtcgaaat tcgccggatt gagctccatt gtctccgcat
      481 cattggagaa tcgctgaccg cgaaggcggg cgatctgacg gttctggttc ttgctccagc
      541 ccccggcggg ggtctggaag ctgacgatcg cgtcggcgac ggcgcgggcc tcgggcgtgg
      601 cgtaccacgc gtcctcgcgg tcgagcggca tgttgccttg ggtgccgccg acggccatcg
      661 gtggcggcgg gacgaccgca ccggccggca gttccagcgc cagggccgcg cggtcgagga
      721 gcgcctgggc atgggaacgg tcgagatagg cggcccaggc gtcccgctcc ggctccggca
      781 accgggcgat gcgcgccgcg gtcagcgatt gggcggggac gttgcgccgg atgacctcgg
      841 catgggcggc gggagctagg gcgaggctca tcagaagcgg aagcagagcg atggcccgcg
      901 tgcgagatgc tgacatggag gccgtccccg gttcgaagca gcagtcgcgc tgctttctca
      961 ttatgctgca tgatatgcta tatgatgaga tcaaacggtc aactcacccg gccgcgaggg
     1021 gacgcccgcg ttggaaaaga tcgcgttctg catgcagctg aatcccggcg ccgaggcgga
     1081 gtacagggcg cgtcacgacg ctctctggcc cgacctgcgg gacgccctgc gcctggccgg
     1141 ggttcgcgac tattcgatcc atctcgagcc cggcaccggt cgactgttcg caaccctctg
     1201 gcgtagcgac gatcaccaaa tggacgctct gcccgacctc ccggtcatga aacgctggtg
     1261 gaggcacatg gcggacctga tgagcaccga ctcctccggc gccccgctcc agacgccgct
     1321 gatcgagatg tttcatctgg agtagggagg cgtcgtgcct tgcttgacgg ccttgctcgc
     1381 cgccctctag ggtgaatcca aataatgaac cgcatggtgc aaatgtggga acgggacgga
     1441 acctcatcgt cagcctcggc tggcctccaa tcccttccga ccttcatcgc ctgttcgtag
     1501 tggaggtctg accgtgcccg ccaatccctt cctcggcgtg atcttccact ggctgggcgg
     1561 tctcgccgcc gccagcttct acgtccccta tcgcggcgtg aagcgctggt cctgggagat
     1621 ctactggatc accggcggcg tcttctcctg ggtcgtcgcg ccctggttct tcgcctctat
     1681 ccagacccat gatctgttcg gggtcctgtc ggcgacaccg tcttcgacca tcggctactg
     1741 catcctgttc ggcggcctgt ggggtttcgg cggtctgacc tacggcctga cgatgcgcta
     1801 cctcggcctg tcgctgggca tggccgtcgt gctgggcctc tgcaccgtgt tcgggaccct
     1861 gatcccgccg atcttccagg ggaccttcgt ctcgaccctg atcgacagcc cctcggggaa
     1921 catcgtcctt ctgggtcttc tggtgaccct cgcaggaatc atcgtcgtcg ccctggccgg
     1981 agcgaagaag ggcgagcccg tcgacaacgc cgccattgcc gaattcgact tcaaaaaagg
     2041 catcggcgtc gccgtcttct ccggcgtcat gtcgtcctg
//
LOCUS       DASVVN010000293         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_142972,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000293 DASVVN010000000
VERSION     DASVVN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142972"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VF686_04660"
     CDS             complement(<1..967)
                     /locus_tag="VF686_04660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8661137.1"
                     /translation="MSISALIRRGWLSLMLVAALALTPTTAPLVAQSSDNARYAAIVV
                     DAESGEVLFSRYADSRRYPASITKVMTLYLTFEALSEGKVKLDDVLTVSSHAASQPPS
                     KLGLGAGQTITLDDAMRATAVRSANDMAVAIAEHVGGSESRFAARMTAKARELGMGQT
                     QYVNANGLPDSRQITTARDLAVLARAVMRDYPQYYRYFGLHDWAYNGRDYRNTNGLLR
                     GGSGYDGLKTGYTNASGYNLAASSVRDGKRLITIVLGGRTTASRNAHVAALMDTGFDV
                     ERARARGETIQIAQSFFEQRGFGIGQELPSGIEYASAATEDAGEEG"
     gene            1260..1766
                     /gene="phaP"
                     /locus_tag="VF686_04665"
     CDS             1260..1766
                     /gene="phaP"
                     /locus_tag="VF686_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164441.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEX8661138.1"
                     /translation="MADNTAETVKKTVETAASNVKASAEKAQATFQANAEQAQAAGAK
                     AFRDVADKSAAGISELNAQGKQNLEALVASAAAAQKGVETLSAQSVAFTKKSWEDGVA
                     AAQSLSQARSIQELLELQTTWAKSATEAYLAEVTKATDVLTASVKDSFKPINERVTAS
                     VEKFQAAR"
     gene            complement(1868..2284)
                     /locus_tag="VF686_04670"
     CDS             complement(1868..2284)
                     /locus_tag="VF686_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8661139.1"
                     /translation="MTTTDAAPTIGRLAKAGGVGVETVRYYQRRGLLETPARDGAGPE
                     GGVRRYGPSDLRRLRFIRAAQAAGFTLEQIGELLALDSGTDRARARTLAEERIHALDV
                     RIAELESARTALRRLAADCGAGVAGPCPIIAAFEAG"
     gene            2357..>3061
                     /locus_tag="VF686_04675"
     CDS             2357..>3061
                     /locus_tag="VF686_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX8661140.1"
                     /translation="MNTRPVAVLYRMVMPDHICPYGQKSLWLLRRKGFEVEDHHLKTR
                     AGTDAFKAEHGVRTTPQTFIGGVRIGGHDDLRRHFGQKVPEAGATSYTPVLAVFAVAA
                     LIALAIDWLSMGRITAMAAPNFIAVAMCLLAMLKLQDVEKFSTMFLNYDLLARRWVPY
                     GYVYPFAELGAGVLMLAGALTWLSAPVALFIGGVGAVSVFKAVYLDKRELKCACVGGA
                     SNVPLGFVSLTENLMMV"
BASE COUNT          504 a         1030 c         1028 g          499 t
ORIGIN      
        1 cgccctcttc cccggcgtct tccgtggcgg ccgaggcgta ttcgattccg ctcgggagtt
       61 cctggccgat gccgaagccg cgctgttcga agaagctctg ggcgatctgg atggtctcgc
      121 cgcgcgcgcg ggcgcgttcg acatcgaagc cggtgtccat cagcgcggcg acatgggcgt
      181 tgcggctggc ggtcgtgcgg ccgcccagaa cgatggtgat cagccgtttc ccgtcccgaa
      241 cggacgaggc cgccagattg tagcccgagg cgttggtgta gcccgtcttc aggccgtcat
      301 agccggaccc gccgcggagc aggccgttgg tgttgcgata gtcgcggccg ttgtaggccc
      361 agtcgtgcag gccgaaatag cgatagtact gcggatagtc ccgcatcacg gcgcgggcca
      421 gaacggcgag gtcgcgcgct gtggtgatct gccggctgtc gggcaggccg ttggcgttga
      481 cgtactgggt ctggcccatg cccagttcgc gggctttcgc cgtcatgcgg gctgcgaagc
      541 gggattcgga gccgccgacg tgttcggcga tggcgacggc catgtcgttg gcgctgcgca
      601 cggcggtcgc gcgcatggcg tcgtccaggg tgatggtctg gccggcgccg aggcccagtt
      661 tggacggcgg ctggctggcg gcgtgggacg acacggtcag gacgtcgtcc agcttgacct
      721 tgccctcgct gagcgcctcg aaggtcaggt acagggtcat caccttggtg atggaggcgg
      781 ggtagcggcg gctgtcggca tagcgcgaga acagcacctc gcccgactcg gcgtcgacga
      841 cgatggcggc gtagcgtgcg ttgtccgagg actgggcgac caggggggcg gttgtcggcg
      901 tcagggccag ggcggccacc agcatcaacg acagccatcc acggcggatc agggcggaga
      961 tcgacaaagg caggcctcac aaagacatta gcgcgacggc ggccccacca cgcgacgctg
     1021 acgaaaggct aacatgcgtc atcggggttt cgtgagggta aacaagccgt caacggattt
     1081 gtgaagcggc ttcgcctgta aggcgctgcg aagccggtat gaagttattg tgcaccgcac
     1141 aaaatcctct tgaccttcct tcgcacttgc accatatagc cgtctgtcgc gatggatccg
     1201 ctccttcgcg aaaccaatca ggcggtcccc cgaccgcaaa aaacgatcag gagactatca
     1261 tggctgacaa caccgccgag accgtcaaga agaccgtcga aaccgccgcc agcaacgtca
     1321 aggcttcggc cgagaaggcc caggccacgt tccaggccaa tgccgaacag gcgcaagccg
     1381 ccggcgccaa ggccttccgc gacgtcgccg acaagtcggc cgccggcatc agcgagctga
     1441 acgcccaggg caagcagaac ctcgaagcgc tggtggcctc cgccgccgcc gcccagaagg
     1501 gcgtcgagac cctgtcggcc cagtcggtcg ccttcaccaa gaagtcttgg gaagacggcg
     1561 tcgccgccgc ccagtcgctg tcgcaggccc gttcgatcca ggaactgctc gagctgcaga
     1621 cgacctgggc caagtcggcc accgaagcct atctggctga agtcaccaag gcgacggacg
     1681 tcctgaccgc ctcggtgaag gacagcttca agccgatcaa cgagcgcgtc accgcctcgg
     1741 tcgaaaagtt ccaggccgcc cgctaaggcg cctcggagtt cagtaagcgt accgaagagc
     1801 ccccggcgga aacgccgggg gctttttgct gcgggcattg aaccatccgt taggcgcgga
     1861 ggcggcttca gcccgcctcg aacgccgcga tgatggggca ggggccggcc acgccagccc
     1921 cgcagtccgc cgccagccgc cgcagggcgg ttcgcgcgct ctccagttca gcgatcctga
     1981 cgtcgagggc gtggatccgc tcttcagcca gggtccgcgc ccgcgcccga tccgtcccgg
     2041 aatccagcgc cagcagttcg ccgatctgct cgagggtgaa gccggcggcc tgggccgcac
     2101 ggatgaagcg cagccgccgc aggtcggacg gaccgtaacg gcgcacgccc ccttcaggcc
     2161 ccgcgccgtc ccgcgccggc gtctccagaa ggccccgccg ctggtaatag cgcaccgtct
     2221 cgacgccgac gccgccagcc ttcgccagcc ggccgatagt gggggcggcg tccgtggtcg
     2281 tcatggcttg actccgtacc atggtacgga ccccagatgg ggctccagcc gttgatcgca
     2341 agctggagcc agtcccatga acacccgtcc cgtcgccgtc ctctatcgca tggtcatgcc
     2401 cgaccacatc tgcccctatg gccagaagtc cctctggctg ctgcgccgca agggcttcga
     2461 ggtcgaggat catcacctca agacccgcgc cgggactgac gccttcaagg ccgaacacgg
     2521 cgtcaggacc acgccccaga ccttcatcgg cggcgtccgc atcggcggtc acgacgacct
     2581 gcgccgccat ttcggccaga aggtccccga ggccggcgcg accagctata cgccggtgct
     2641 ggccgtcttc gccgtcgccg ccctgatcgc cctcgccatc gactggctgt ccatggggcg
     2701 gatcaccgcg atggccgcgc cgaacttcat cgccgtcgca atgtgtctgc tggcgatgct
     2761 gaagctgcag gatgtcgaga aattctcgac gatgttcctg aactacgacc tgctggcccg
     2821 gcgctgggtc ccgtacggtt atgtctatcc gttcgccgaa ctgggcgcgg gggtgctgat
     2881 gctggccggc gccctgacgt ggctgtcggc cccggtcgcg ctgttcatcg gcggcgtcgg
     2941 cgcggtctcg gtgttcaagg cggtctatct cgacaagcgc gagctgaaat gcgcctgtgt
     3001 cggcggcgcg tcgaatgtgc cgctgggctt cgtctccctg accgagaacc tgatgatggt
     3061 c
//
LOCUS       DASVVN010000294         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_35963, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000294 DASVVN010000000
VERSION     DASVVN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35963"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF686_04680"
     CDS             complement(<1..485)
                     /locus_tag="VF686_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX8661141.1"
                     /translation="MVESDGIGKSINGEPGAGEAAQALRAILQTQYLRYMIIGTWAVG
                     LMATVGLSTAALWFVGTVAAGALRGAVEKRISDRVGSGWGLVFPAVATATTASWATAP
                     VLAWFSGAEFGHALAMALLVSGYVLVFAQLRSSPRQAMVISSPYGVAALVIAASLWGT
                     PT"
     gene            complement(575..889)
                     /locus_tag="VF686_04685"
     CDS             complement(575..889)
                     /locus_tag="VF686_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX8661142.1"
                     /translation="MPEGPIATIIREKLTAGLSPERLVIEDDSARHAGHHHEGGMDGQ
                     PGGESHFNLTVVAAAFEGQGRVHRQRAINALLRAELAGPVHALSIRALTPAEAAATLG
                     DS"
     gene            935..1609
                     /locus_tag="VF686_04690"
     CDS             935..1609
                     /locus_tag="VF686_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8661143.1"
                     /translation="MSSEFEYKPRFKDMRIKPPRPEEEAAEADVLHLKPGEKSCQWPD
                     CRAAATARAPKSRERLNDFYDFCQRHAGEYNKGWNFYAGMSDGEVRAAKENEAMTGGR
                     PTWEMKAGKFSREAAAFAAKMGTANTKGAGSWQDSFGLFGRRMKEQTGPAGEDTLRLG
                     KLERAALATLDLDARTDKAGVKARYHEMLKRFHPDLNQGDRGAEAKLQSVIKAYKTLK
                     KAGLAG"
     gene            1681..1914
                     /locus_tag="VF686_04695"
     CDS             1681..1914
                     /locus_tag="VF686_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661144.1"
                     /translation="MRIAVLAAAICLSALAGCEQSYSSSRTVTESNGDVVERTVEVQI
                     GDRRTPPAAEKAGNPPAAPAAPAAADTPVAPEG"
     gene            1990..2418
                     /locus_tag="VF686_04700"
     CDS             1990..2418
                     /locus_tag="VF686_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX8661145.1"
                     /translation="MQVLYTAHAVSTGGGRDEGRSATDDGKIDVLLSLPAEMGGKGGA
                     GTNPEQLFATGYAACYMGALRLVSGKAGTPVGPDTKIHTAVGIGPNTRGEGFNLDIAI
                     KITDHGLDKAVIDDLIQKAQQVCPYSNATRGTVDVARTAE"
BASE COUNT          447 a          892 c          805 g          349 t
ORIGIN      
        1 gtcggcgtgc cccacaggct ggccgcgatg acgagggcgg cgacgccata gggtgacgaa
       61 atcaccatcg cctgccgcgg cgaactgcgc agctgggcga agaccagcac atagcccgac
      121 accaacaggg ccatggccag cgcatggccg aactcggctc ccgagaacca ggccagcacc
      181 ggcgccgtgg cccaggaagc cgtcgtcgcg gtcgccacgg cggggaacac cagtccccag
      241 cccgagccca cgcggtcgct gatgcgtttt tcgacggcgc cgcgcagggc gccggcggcc
      301 accgtgccca cgaaccacag ggcggcggtg ctcaggccga ccgtggccat caggccgacg
      361 gcccaggtgc cgatgatcat atagcggagg tattgcgtct gcaggatcgc acgaagcgct
      421 tgcgcggctt cgccagcgcc cggttcgccg ttgatcgact tgccgatgcc gtcggattcc
      481 accatgcgcg cgccgccccg ttacaatgtg ggccgaacgc tatggcgaag tcgctaagac
      541 cagatgaacg cagaccgatt ctgaaatcgg cgttttacga atccccgagg gtcgcggcgg
      601 cctcggccgg agtcagcgcc cgtatggaca gggcatggac cgggccggcc agttcggcgc
      661 gcagcagggc gttgatcgcg cgttgcctgt gcacccttcc ctgcccctcg aaggccgccg
      721 cgaccacggt cagattgaaa tggctctcgc ctccgggctg cccgtccatg ccgccttcgt
      781 ggtgatggcc ggcgtgccgg gcgctgtcgt cctcgatcac cagccgctcc ggagacaggc
      841 ccgccgtcag tttttcccgg ataatcgtcg caatcgggcc ttccggcatg ggtgtgtcct
      901 tgttggtgac caattcgacc ttacacttgc accgatgtca tccgagtttg aatacaagcc
      961 ccgtttcaag gacatgcgga tcaagccgcc ccgccccgaa gaggaggcgg cggaggccga
     1021 cgtcctgcac ctgaaaccgg gcgagaagtc ttgccagtgg cccgactgcc gcgccgccgc
     1081 cacggcccgg gcgcccaagt cgcgcgaacg gctcaacgac ttctacgatt tctgccagcg
     1141 ccacgccggc gagtacaaca agggctggaa tttctacgcc ggcatgagcg acggcgaagt
     1201 ccgcgcggcc aaggaaaacg aggccatgac cggcggccgc cccacctggg agatgaaggc
     1261 gggcaagttc agccgcgagg ccgccgcctt cgccgccaag atgggcacgg ccaacaccaa
     1321 gggcgcgggc agctggcagg acagcttcgg cctgttcggc cgtcgcatga aggagcagac
     1381 cggcccggcc ggcgaggaca cgctgcgcct cggcaagctg gaacgcgcgg ccctggccac
     1441 gctcgacctc gacgcccgca ccgacaaggc cggggtcaag gcgcgctatc acgagatgct
     1501 gaaacgcttc cacccggacc tgaaccaggg cgaccgcggc gcagaggcca agctgcagag
     1561 cgtcatcaag gcctacaaga cgttgaaaaa ggccggtctg gcgggctgac gccgccttgc
     1621 cggtgtcggc cccccgtgac aatcatggct tgcaggacgc tcggtcctca gggggtttca
     1681 atgcgcattg cggttctggc tgcggccatc tgtctgtccg cgctcgcggg gtgtgaacag
     1741 tcttacagtt cgtcccggac cgtcaccgaa tccaacggag atgtcgtcga gcgcaccgtc
     1801 gaggtccaga tcggcgaccg gcgtacgcct ccggcggctg aaaaagcggg gaacccgccc
     1861 gccgcgcccg ccgcacccgc cgctgccgat acgcccgtcg cgcccgaagg ctgacaaatc
     1921 ccgcgcggcc gcccttcaat acggagcggc tctgcctaac tgtcaggctt ccccgcacag
     1981 gagccgtcca tgcaagtcct ctacaccgcc cacgccgtct cgaccggagg cggccgggac
     2041 gaaggccgct cggccaccga cgacggcaag atcgacgtcc tgctctccct tcccgccgaa
     2101 atgggcggca agggcggcgc cggcaccaat cccgagcagt tgttcgcgac cggctacgcc
     2161 gcctgctaca tgggcgcgct gcggctggtc agcggcaagg ccggcacgcc ggtcggcccg
     2221 gacacgaaga tccacaccgc ggtcggcatc ggccctaaca cccgcggcga aggcttcaac
     2281 ctcgacatcg cgatcaagat caccgaccac ggcctcgaca aggccgtcat cgacgacctg
     2341 atccagaagg cccaacaggt ctgcccctac tccaacgcca cccgcggcac cgtcgacgtg
     2401 gcgcggaccg cggaatagtc ggcctcagga ctccatcccc gacaacccgg tatcgaggtg
     2461 ctgggcgatc agcgcctcga tatcgacggg ctc
//
LOCUS       DASVVN010000295         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_10806, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000295 DASVVN010000000
VERSION     DASVVN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10806"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF686_04705"
     CDS             <1..338
                     /locus_tag="VF686_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8661146.1"
                     /translation="GPILHVCRYEPSKLRETASKLARRGYGLTLGVHSRIEAFAEEVT
                     RLVPAGNVYINRSIIGAVVGVQPFGGEGLSGTGPKAGGPYSLIRYASEKAISNNISAQ
                     GGDPALLNL"
     gene            complement(342..638)
                     /locus_tag="VF686_04710"
     CDS             complement(342..638)
                     /locus_tag="VF686_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661147.1"
                     /translation="MKVTNEDLDQLWKALRMLGWGAVALIMTAPLVAMQFTSEVNWTV
                     SDFVFAGVMLIGGGAVIELVAWRVRNPVIRIGFSLFVIAIVALIWVEGAVGIFH"
     gene            complement(733..1650)
                     /locus_tag="VF686_04715"
     CDS             complement(733..1650)
                     /locus_tag="VF686_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX8661148.1"
                     /translation="MSRSITIVEVGPRDGLQNEKAILEPAVRVDLVRRLEAAGARRIE
                     AVSFVHPKYVPQMAGAEEVMAALPKEAGRSRIGLVLNGKGYDRALGTAVDEVNVAMSA
                     TDGFGLKNQGLTVDQQVQMLADIVAGRANAEGSPGAAPSLSATLSCVWGCPFDGEVST
                     DQVADLVGRVAALGVGEIALADTIGVGDPWSVTKKVEAARKAAPDAALRLHFHDTRNT
                     GLANAFAGVEAGVDVLDASVGGIGGCPFAPGATGNIATEDLVYMLERAGFSTGYDLDA
                     LIGTARWIGEKIGRPAPSALSRAGGWPKG"
     gene            complement(1647..3332)
                     /locus_tag="VF686_04720"
     CDS             complement(1647..3332)
                     /locus_tag="VF686_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8661149.1"
                     /translation="MTGADFDRYIAGWRGPVLAALIALFAGLPVLMLLPPLDRVESRY
                     AQATSQMLESGDYVDIRYQDEPRWKKPVGIYWMQAVAVAVTSDVENRSIGPYRIPSLL
                     GAMLAAAACAWAGAAMFGQRAGFLAGAIMGASFLLSTEAGIAKTDAMLCGAVTLAMAG
                     LARIYLAARAGEAPRRPWKLAFWLGLGLSILIKGPIGLLVIAPAILALSVWDRDVSWL
                     KRLGWGWGLPLVALIVGPWAIAITIATDGGFWREAIAGDLAPKVAGGDEGHGVLPGFY
                     LLIAPILFFPATLLLPAAVSTGWHRRAEPAIRFLICWVVPAWIIFELTPTKLAHYTLP
                     TFGGLALLAAAATARPIGKISRYAGVGLTLFVGGILAVGSVYLLTEYGKSTAQTWATL
                     TIVFAILASLVGAFLMMHRAAVTALIASLALGVMAHAALAGTLRQLRPLAVAPQLEKV
                     LERADLHPRQGRAGPVAITGFHEPSFVFLTGKDTELTDAAGAARALAEGRPAIVEEHD
                     ADAFREAAAQLGVTGRAVGVVNGHNYSKGDDVSLTVYAPPGVAAASSSADSGR"
BASE COUNT          539 a         1279 c         1091 g          468 t
ORIGIN      
        1 tcggccccat cctgcacgtc tgccgctacg agccatccaa gctccgggag acggcctcga
       61 agctggcccg gcgcggctac ggcctgaccc tcggcgtgca cagccgcatc gaggccttcg
      121 ccgaggaggt cacccgtctg gtcccggccg gcaacgtcta tatcaaccgc tcgatcatcg
      181 gcgcggtggt cggcgttcag cctttcggcg gcgaaggcct gtccggcacc gggccgaagg
      241 ccggcggccc gtacagcctg atccgctacg cctccgaaaa ggccatcagc aacaacatct
      301 ccgcccaggg cggagacccg gccctgctga acctctgacg cctagtggaa gatccccacc
      361 gcgccctcga cccagatcag ggcgacgatg gcgatgacga acagcgagaa gccgatccgg
      421 atcaccggat tgcgcacccg ccatgcgacc agttcgatca ccgccccgcc gccgatcagc
      481 atcaccccgg cgaagacgaa gtcgctgacg gtccagttga cctcgctggt gaactgcatg
      541 gcgaccagag gcgcggtcat gatcagggcc accgctcccc agccgagcat gcgcagggcc
      601 ttccacagct ggtccagatc ctcgttcgtc accttcatgt cgtctctccc gtgaacatcg
      661 acgaccggac catgcggcgg ccgccggctg agcggcatga gcgaaatgtg agcagttgct
      721 gaaaagcggc cgtcagccct tcggccaacc gccggcccgg ctcagggcgc tcggcgccgg
      781 gcggccgatc ttctcgccga tccagcgcgc ggttcctatc agggcgtcga ggtcgtagcc
      841 ggtggagaag cccgcccgtt ccagcatgta gacgaggtcc tcggtggcga tattgcccgt
      901 ggcccccggc gcgaacgggc agccgccgat cccgccgacc gaggcgtcca gcacgtccac
      961 ccccgcctcg accccggcga aggcgttggc cagccccgtg tttcgcgtgt cgtggaaatg
     1021 cagccgcagc gcagcgtccg gcgcggcctt gcgggcggcc tccaccttct tcgtcacgct
     1081 ccacggatcg ccgaccccga tggtgtcggc caaagcgatc tcgcccacgc ccagcgctgc
     1141 gacgcgcccg accagatcgg cgacctgatc cgtcgacacc tcgccgtcga acgggcagcc
     1201 ccagacgcag gacagggtcg ccgacagcga cggcgccgcc cccggcgagc cctccgcatt
     1261 ggcgcggccc gcgacgatgt ccgccagcat ctgcacctgc tgatcgaccg tcagcccctg
     1321 attcttcagc ccgaagccgt cggtggcgga catggccaca ttgacctcgt ccaccgccgt
     1381 gcccagcgcc cggtcatagc ccttgccgtt cagcaccaga ccgatgcggc tgcggcccgc
     1441 ttccttcggc agggcggcca tgacctcttc ggcccccgcc atctgcggca cgtatttcgg
     1501 atgcacgaag ctgaccgcct cgatccgccg cgcccccgcc gcctccagcc gccggaccag
     1561 atcgacgcgg acggcgggct ccaggatggc cttctcgttc tgcaggccgt cgcgcggacc
     1621 gacctcgacg atggtgatgc ttcggctcat cgaccggaat ccgcggagga actcgccgcc
     1681 gccacgcccg gcggggcgta gaccgtcagg ctgacgtcgt cgcccttgga atagttgtgg
     1741 ccgttgacca cgcccacggc gcggccggtg acgcccagct gcgcggcggc ctcgcggaag
     1801 gcgtcggcgt catgctcctc gacgatggcg ggccgcccct cggccagcgc gcgcgcggcc
     1861 cccgcggcgt ctgtcagttc cgtgtccttg ccggtcagga agacgaagct gggctcatgg
     1921 aagccggtga tggccacggg cccggcccgg ccctgacgcg gatgcaggtc ggcccgttcc
     1981 agcaccttct ccaactgcgg cgccacggcc agcggccgca gctgacgcag ggtgccggcc
     2041 agcgccgcat gggccatgac gcccagcgcc agcgatgcga tcagcgccgt caccgccgcc
     2101 cggtgcatca tcaggaaggc tccgaccaga ctggccagta tggcgaagac gatggtcagg
     2161 gtcgcccagg tctgggcggt cgacttgccg tattcggtca gcagatagac cgacccgacc
     2221 gccagaattc ctccgacgaa cagggtcagc ccgaccccgg cgtagcgcga tatctttccg
     2281 atcggccgcg ccgtggcggc ggccgccaga agcgccagtc cgccgaacgt cggcagggtg
     2341 tagtgggcca gcttggtcgg ggtcagctcg aagatgatcc aggccggaac cacccagcag
     2401 atcaggaacc ggatcgccgg ctcggcccgc ctgtgccagc cggtcgagac ggccgccggc
     2461 agcagcagcg tcgccgggaa gaacaggatc ggcgcgatca gcaggtagaa gccgggcagg
     2521 acgccatgcc cctcgtcgcc gcccgccacc ttgggcgcca gatcgcccgc tatggcctcg
     2581 cgccagaagc cgccgtcggt ggcgatggtg atcgcgatgg cccacggccc gacgatcagg
     2641 gccaccagcg gcaggcccca gccccagccc agccgcttca gccacgacac gtcccggtcc
     2701 cagaccgaca gcgccagtat ggcgggcgcg atgaccagca ggccgatcgg ccccttgatc
     2761 aggatggaca ggcccagccc cagccagaac gccagtttcc acggtcttcg cggcgcctct
     2821 ccggcccgcg ccgccagata gattcgcgcc agcccggcca tggccagggt caccgccccg
     2881 cacagcatgg cgtcggtctt ggcgatcccc gcctcggtcg acagcaggaa gctggccccc
     2941 atgatcgcgc cggcaaggaa ccccgccctc tggccgaaca tcgccgctcc cgcccaggcg
     3001 cacgcggccg cggccagcat ggcgcccagc agcgaaggga tgcggtaggg accgatgctc
     3061 cggttctcga cgtcggaggt caccgccacc gccacggcct gcatccagta gatgccgacc
     3121 ggcttcttcc accgcggctc gtcctgatag cggatgtcga catagtcgcc gctctcgagc
     3181 atctgcgacg tggcctgggc gtaccggctc tcgacacggt ccagcggcgg cagcagcatc
     3241 agcaccggca ggcccgcgaa cagggcgatc agcgccgcca gaacggggcc ccgccagccg
     3301 gcgatgtatc ggtcgaagtc ggcgcctgtc atgttcatct tcctaacacg gcgtttcggc
     3361 ccgcgtcagg cgtgatt
//
LOCUS       DASVVN010000296         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_195832,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000296 DASVVN010000000
VERSION     DASVVN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195832"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VF686_04725"
     CDS             complement(<1..678)
                     /locus_tag="VF686_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8661150.1"
                     /translation="MSITTVGLDADDTLWHNETIFRLTQDRFVALLAEHADRATLEAR
                     MAETEKRNLRLYGYGVKGFTLSMIETAMELTGGEAPASVVREILAAGREMLAHPVETL
                     PGVDEALATLSESYRLVLITKGDLLDQERKLAASGLGDLFAAVEIVSEKDRHTYDRIF
                     ARHGTGAQEAVMAGNSMRSDVLPALEAGAWAVHIPYSITWAHEVAEAPAIQTRYGALN
                     SIGELPGW"
     gene            811..1656
                     /locus_tag="VF686_04730"
     CDS             811..1656
                     /locus_tag="VF686_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661151.1"
                     /translation="MEDSDGQPKEAVMTFFPRYPTPPDHGNKREGDNAFSPLGERRAY
                     AAAYESVTFGLVTRAGRELIVTLAAQPVFDLDRSTPVSRRIRRIVRHRGGESALAALG
                     RRTLEPVDLKRIDLQTLNHGLDLLHLGANDIGVLPAFWRTVASSRGRFALLCTELQHG
                     SAPGNLMVEVMGGLEQAPPEAIDETISHFETESLGVILHIAPDAGLVRRLMGVRARCL
                     AIDFAGVAHDGPLEWQAAQDLIMAARQTCDQVMLLNLRPDRGLAAQAAGATHAVFAGM
                     ETITV"
     gene            1700..>1908
                     /locus_tag="VF686_04735"
     CDS             1700..>1908
                     /locus_tag="VF686_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX8661152.1"
                     /translation="MQATLPSRLYGCPEAWARERSAVFGRAWLFLGHEAEAPAAGDWI
                     ASDVAGHRLMALRGKDGVLRAFHNV"
BASE COUNT          310 a          638 c          618 g          342 t
ORIGIN      
        1 ccaccccggc agttcgccta tgctgttcaa agcgccatat cgggtctgga ttgcgggagc
       61 ctcggcgact tcatgagccc aggtgatcga atacgggatg tgtacggccc acgcgccggc
      121 ttcgagggca ggcaggacat ccgatctcat cgaattgcca gccatcacgg cttcctgggc
      181 tccggttcca tgacgggcga agatgcggtc gtacgtatgg cgatccttct cggatacaat
      241 ctcgaccgcg gcgaacaagt cgcccaagcc tgatgcggcc agcttacgtt cctggtccag
      301 caagtcgccc ttggtgatca gcaccagacg ataggattcc gacagcgtgg cgagggcttc
      361 gtccacgccg ggcagggtct cgaccggatg agccagcatt tcccgccccg cggccaggat
      421 ttcgcgcacg accgaagccg gggcctcacc gcccgtcagt tccatggcgg tctcgatcat
      481 cgacagggtg aaacccttga cgccgtagcc gtatagacga aggttccgct tctcggtttc
      541 cgccatccgc gcctccagcg tggcgcgatc ggcatgttcg gccagcaggg cgacgaaccg
      601 gtcttgcgtc agccggaaga tggtctcgtt gtgccagagg gtgtcgtcgg catcgaggcc
      661 gaccgtggtg atgctcatcc cgctccccta acatcggaac ccgcatccgg ggagggggcg
      721 gcgatggcct gagacgggga ccgtcatagt caacaatcct tgaccaagtt tatcagggtt
      781 acgttcttgt ctgcgcgtta tcctgacggc gtggaagact ccgacggcca gccgaaggaa
      841 gcggtcatga ccttctttcc aaggtatccg acgccccctg accacgggaa caaacgcgag
      901 ggagacaacg cgttctcccc gctgggcgag cgccgcgcct acgccgccgc ctatgaaagc
      961 gtgaccttcg gtctggttac ccgcgcaggc agggaattga tcgtgaccct cgccgcccag
     1021 cccgttttcg accttgatcg atcgacgccg gtcagccggc gtattcgccg tatcgtccgg
     1081 caccgcggcg gtgagtcggc cctggccgcc ctgggtcgca ggacgctgga acctgtcgac
     1141 ctgaagcgca tcgatcttca aaccctgaac cacggtctcg acctgctgca cctgggggcc
     1201 aacgacatcg gggtgctgcc ggctttctgg cgcaccgtgg cgtcgagccg gggacggttc
     1261 gccctgcttt gcacagaact tcaacacggc tccgctccgg ggaacctcat ggtcgaggtg
     1321 atgggcgggc tggaacaggc gccgcccgag gcgatcgacg agaccatctc gcatttcgaa
     1381 accgaatccc tcggcgtgat cctgcatatc gcgcccgatg caggccttgt ccgccggctg
     1441 atgggcgtcc gggcccgctg cctggccatc gacttcgcgg gggtcgccca cgacgggccg
     1501 cttgagtggc aagccgctca ggacctgatc atggcggcgc gccagacctg cgaccaggtc
     1561 atgctgctga acctgcgtcc cgaccggggc ctggccgccc aggctgccgg tgccacccac
     1621 gccgtattcg ccggcatgga gaccatcacc gtctagagag gcggttgacg ccgtcgccgc
     1681 agcggggcct tgatccggca tgcaggccac gcttccctcc cgactatacg gctgtcccga
     1741 agcctgggcg cgcgagcggt cggccgtatt cggccgcgcg tggctgtttc tggggcatga
     1801 ggccgaggcg cccgcggccg gggactggat cgcgtccgac gtcgccggtc atcgtctgat
     1861 ggccctgcgc ggcaaggacg gtgtgctgcg ggccttccac aatgtctg
//
LOCUS       DASVVN010000297         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_143185,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000297 DASVVN010000000
VERSION     DASVVN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143185"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="dgt"
                     /locus_tag="VF686_04740"
     CDS             complement(<1..470)
                     /gene="dgt"
                     /locus_tag="VF686_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX8661153.1"
                     /translation="MAERKGPQSNEQPTLIPERAPYAEYPEHSQGRLTPEPISRTRNA
                     YARDRDRIIHATAFRRLKEKTQVFVAHEGDHYRTRLTHSLEVAQIARSLAHALRLDTD
                     LAETVALAHDLGHPPFGHAGEDELQAQMEPWGGFDHNVQTFRVVTRLEHRYPQF"
     gene            492..869
                     /gene="erpA"
                     /locus_tag="VF686_04745"
     CDS             492..869
                     /gene="erpA"
                     /locus_tag="VF686_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX8661154.1"
                     /translation="MSTVQSAPAFQISPSAGGHGLTLSETAARRLETLGRDEGHPVML
                     RVAVDGGGCSGFQYRFELVERAEADDIRIEADGQAALVDPVSLPFLKGSEIAFVDDLA
                     GAQFVVRNPNAASSCGCGVSFSI"
     gene            948..1622
                     /locus_tag="VF686_04750"
     CDS             948..1622
                     /locus_tag="VF686_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661155.1"
                     /translation="MEALFTPTLILHITCGLAASLVGLAPIFTRKGSRLHRLSGRLFA
                     GLMATLLACAWLMTALHFSSYFLALSATATMTLFSGVRVLGRKRPDLRREDRAKPLDW
                     VVTLGVVGVGFWVLFLVISGQTGDLAAVSSALAYGALVYGGWDLWRFARPTDWPFSPN
                     LWNYEHLTKMLGAYGAVLAAFSGNFLTFIPTPWSQLWPSILFQSVTVIWIAVLIVRRR
                     RSPAFA"
     gene            1571..>2460
                     /locus_tag="VF686_04755"
     CDS             1571..>2460
                     /locus_tag="VF686_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8661156.1"
                     /translation="MDRRPDRAPSPLAGVRLSRVTTAEWVGIFTTYSYGMTTVCAAWF
                     MRRAVEAKGGHLGVADSLIWQGATYALWLPVAVAVWLIIRRFGAGLAALAALTVAGLA
                     IVPLAALGGAAVDLAFAAVPASELAGRALARTPVAILLYTAICAVGAAAAHRSRAVEA
                     QAHARTLKAALHAAREALAGNDAPVERLMVMVGARRAPVLLDEVEWFASAGNYVVVHW
                     DDREGLIREPLKALEQRLDRRVFARSHRSTVVNLARVRETRSLSDGSWKLTMASGTEL
                     VASRTYRDAVMARLGRQDASV"
BASE COUNT          303 a          843 c          871 g          443 t
ORIGIN      
        1 tcgaactggg gatagcggtg ctccagccgg gtcacgacgc ggaaggtctg gacgttgtgg
       61 tcgaacccgc cccacggctc catctgcgcc tgcagctcgt cctcgcccgc atggccgaac
      121 ggcggatggc cgaggtcgtg ggccagagcc acagtctcgg ccagatcggt gtccagccgc
      181 agggcgtggg ccagggatcg cgcgatctgg gccacctcga ggctgtgggt caggcgggtg
      241 cggtagtggt cgccctcgtg cgccacgaag acctgggtct tctccttcag gcgacggaag
      301 gcggtggcgt ggatgatccg gtcgcggtcg cgggcgtagg cgttgcgcgt gcggctgatc
      361 ggctcgggcg tcagccggcc ctggctgtgc tcgggatatt cggcgtaagg cgcgcgctcg
      421 gggatcaacg tcggctgctc attcgactgc gggcctttgc gttcggccac ccgcgcctca
      481 tataggcccc tgtgagcacc gtccaatccg cccccgcctt ccagatatcg ccgtccgccg
      541 gcggtcacgg cctgaccctg tccgaaaccg ccgcccggcg gctggagacg ctgggcaggg
      601 acgagggcca tccggtcatg ctgcgcgtcg ccgtcgacgg cggcggctgt tcgggcttcc
      661 agtaccggtt cgagctggtc gagcgcgcgg aagccgacga catccgcatc gaggccgatg
      721 gtcaggcggc gctggtggac ccggtttccc tgcccttcct caagggttcc gagatcgcct
      781 tcgtcgatga tctggccggg gcgcagttcg tggtgcgcaa cccgaacgcc gcctccagct
      841 gcggctgcgg cgtcagcttc tcgatctagc ccgcgcttca tcccggccag ccgccgttcc
      901 tcccgcgcgc cgtgacgggc cccggcagtc ggcctcagcg tcggggcatg gaagcgctct
      961 tcacccccac tctcatcctg cacatcacct gcggcctcgc ggcgtcgctg gtaggcttgg
     1021 ccccgatctt cacccgcaag ggctcacggc tacaccgcct gtccggccgg ctgttcgccg
     1081 gactgatggc aacgctgctg gcctgcgcct ggctgatgac cgccctgcat ttcagcagct
     1141 atttcctcgc cctctcggcg accgcgacca tgaccctgtt ctcgggcgtc cgggtgctgg
     1201 ggcgcaagcg accggatctt cggcgcgagg atcgtgcaaa gccgctggac tgggttgtga
     1261 ctctcggtgt cgtgggcgtc ggcttctggg ttctgttcct cgttatcagc ggccagaccg
     1321 gtgatctggc ggccgtgtcg tcggccctcg cctatggggc gctggtctat ggcggctggg
     1381 acctgtggcg gttcgcacgg ccgacggact ggcccttctc gcccaacctg tggaactatg
     1441 agcatctgac caagatgctg ggagcctatg gcgcggtgct cgcggccttc tccgggaatt
     1501 tcctcacttt catccccact ccatggagcc agctctggcc cagcatcctg ttccagtcgg
     1561 tgaccgtgat atggatcgcc gtcctgatcg tgcgccgtcg ccgctcgccg gcgttcgcct
     1621 gagccgggtc acgacggccg aatgggtcgg gattttcacg acctacagct acggcatgac
     1681 gacggtctgc gccgcgtggt tcatgcgacg ggctgtagag gcgaagggcg gtcatctggg
     1741 cgtcgccgac tccctgatct ggcagggcgc gacctacgcc ctgtggctgc ctgtcgcggt
     1801 cgccgtctgg ctgatcatcc gccggttcgg cgccggactg gcggcgctgg ccgccctgac
     1861 ggtcgccggc ctcgccatcg tgccgctggc ggcgctgggc ggggccgcgg tcgatctggc
     1921 cttcgccgcg gttccggcct cggaactggc aggtcgggcg ctggcccgta ctccggtggc
     1981 gatcctgctc tacaccgcca tctgcgccgt cggcgcggcg gcggcgcacc gcagccgggc
     2041 ggtcgaggcg caggcccatg cgcggacact gaaggccgcc ctgcacgcgg cccgggaggc
     2101 gctcgccgga aatgacgctc cggtcgagcg gctgatggtc atggttggcg cccgacgcgc
     2161 gccggtcctg ctggacgagg tcgaatggtt cgcatcggcg ggcaactatg tcgtcgtcca
     2221 ttgggacgac cgcgagggcc tgatccgcga accgctgaag gcgctggagc aacggctgga
     2281 ccgccgtgtg ttcgcgcgca gccaccgctc gacggtggtc aatctggcgc gggtgcggga
     2341 gacgcggtcc ctgtccgacg gctcatggaa gctgaccatg gccagtggga cggaactggt
     2401 cgccagccga acctatcgcg acgccgtgat ggcgcggctg ggccgtcagg acgccagcgt
//
LOCUS       DASVVN010000298         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_354981,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000298 DASVVN010000000
VERSION     DASVVN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354981"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF686_04760"
     CDS             <1..656
                     /locus_tag="VF686_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8661157.1"
                     /translation="DAEEAELTRKLEALQAASSRQLVVATVADLEGYPIEDYGYQLGR
                     AWGIGQAGKNNGVLLIIAPNERKVRIEVGYGLEGTLTDALSALIIQNDVLPSFKQGAY
                     ERGIEQGVDAIDRQLRLDPAEAQARAAAAERPKSSPPIGVAAVIAMIFIMLMLRMIGG
                     GGRGRRRPSGLGPVLIWAASEAMRDSSRGGGGGFGGGGGGFGGGGGSFGGGGASGGW"
     gene            650..1342
                     /locus_tag="VF686_04765"
     CDS             650..1342
                     /locus_tag="VF686_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8661158.1"
                     /translation="MVIKLTTDVHDRVAEAIRVAETRTSGEIFCVMARRVSAYRDISL
                     GWAAAAALILPLGLIPFGFEAAWIPGLGASWEAAHLAARDVTVGQALGAYALIQATVF
                     LIVFLLTSIPAVSRIVTPRSIRQARVRKAAMQQFLAHGLHVTEARTGVLIFAALGDHQ
                     VEVVADEGIHGKVDQAVWADAVEVLAKGLRRGDPAGGFVDAVALCGAVLAEHFPPAPL
                     NRNEVEDKLVVI"
     gene            1428..>2066
                     /locus_tag="VF686_04770"
     CDS             1428..>2066
                     /locus_tag="VF686_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8661159.1"
                     /translation="MPRLLTYNVHRCVGVDRRLDVARVAGVIAEHEPDIVCLQELDVG
                     RRRTGFVDQAQSIADQLAMTFHFHAAMRVEAEQYGDAILTARPERLVRSGPLPTIRGV
                     PGLEPRGALWAAIDFDGVTLNVINTHLGLVPREQRLQAAALTGPDWLGHPDCIGPTLL
                     TGDFNATSVSRPYQMLVARLADCQRTLGLRPSVKTFPSSFPAIRIDHCFTSPE"
BASE COUNT          309 a          704 c          711 g          342 t
ORIGIN      
        1 ccgacgcgga ggaagccgag ctcacccgca agctggaggc gctccaggcg gcgagcagcc
       61 gccagctggt ggtcgcgacc gtcgccgacc tcgaaggcta tccgatcgag gattacggct
      121 atcagctggg ccgtgcctgg ggcatcggcc aggccggcaa gaacaacggc gtgctgctga
      181 tcatcgcgcc gaacgaacgc aaggtgcgga tcgaggtcgg ctacggcctc gaggggaccc
      241 tgaccgacgc cctgtccgcc ctgatcatcc agaacgacgt cttgccctcg ttcaagcagg
      301 gggcctacga acgcggcatc gaacagggcg tggacgccat cgaccggcag cttcggcttg
      361 atcccgccga ggcccaggcg cgcgcggcgg cggccgagag gcccaaatcc tcgccgccca
      421 tcggcgtcgc cgccgtcatc gccatgatct tcatcatgct gatgctgcgg atgatcggcg
      481 gcggtggtcg gggacgtaga cggcccagcg gtctcggccc cgtgctgatc tgggcggcct
      541 ctgaagccat gcgcgacagt agtcggggcg gcggcggcgg gttcggcggc ggaggcggtg
      601 gatttggcgg cggcggaggc agtttcggcg gcgggggcgc atcgggcgga tggtgatcaa
      661 actgacgacc gatgtgcatg accgcgtggc agaggcgatc cgcgtcgcgg agacacgcac
      721 ctccggtgaa atcttctgcg tcatggcgcg gcgtgtctct gcataccggg atatctctct
      781 cggctgggcg gcggcggccg cgctgatcct gcccttgggc ctgatcccgt tcggcttcga
      841 ggccgcatgg attccgggcc tcggggccag ctgggaggcc gcccatctgg ccgcccgcga
      901 cgtgacggtg ggacaggcgc tgggcgccta cgcgctgatc caggcgacgg tcttcctgat
      961 cgtcttcctg ctgacgtcca ttcccgcggt gtcccggatc gtcacgccgc gcagcattcg
     1021 tcaggcgcgc gttcgcaagg cggcgatgca gcagttcctc gcccatggcc tgcatgtcac
     1081 ggaggcgcga accggggtgc tgatcttcgc cgccctcggc gatcatcagg tcgaggtcgt
     1141 cgccgatgaa ggcatccacg gcaaggtcga ccaggccgtc tgggcggacg ccgtcgaggt
     1201 tctggcgaag ggcctgcggc gcggcgaccc cgccggaggc ttcgtcgacg ccgtggcgct
     1261 ctgcggcgcg gtgctggccg agcatttccc gcccgctccg ctcaaccgga acgaggtcga
     1321 ggacaagctg gtggtgatct gatccaaacg cgcgccaccg ctttggtgtc gcactcaatg
     1381 tggtattggc tcacccccgc cactccgctg gacccgcttc agactccatg ccccgcctgc
     1441 tgacctacaa tgtccaccgt tgcgtcggcg tcgaccgcag gcttgacgtc gcgcgggtcg
     1501 cgggcgtcat cgccgagcat gagcccgata tcgtctgcct ccaggaactg gacgtcggcc
     1561 gaaggcggac gggcttcgtc gaccaggccc agtccatcgc cgatcagctg gcgatgacct
     1621 tccatttcca cgccgccatg cgcgtggaag cagagcaata tggcgacgcc atcctgaccg
     1681 cgcgtcctga acgtctggtg aggtcgggcc cgctgccgac gatccgcggt gtgcccggac
     1741 tggaaccgcg cggcgcgctg tgggcggcga tcgacttcga cggagtcacc ctgaatgtga
     1801 tcaacaccca tctggggctg gtcccacgcg aacagaggct tcaggcggcg gctctgacgg
     1861 gtcccgactg gctcggccat cccgactgca tcggcccgac gttgctgacc ggcgacttca
     1921 acgccacctc ggtgtcccgc ccgtatcaga tgctggtggc caggctggcg gactgtcagc
     1981 gcaccctggg cctgagaccg tcggtcaaga ccttcccgtc cagcttcccg gccatacgca
     2041 tcgaccactg tttcaccagc cctgaa
//
LOCUS       DASVVN010000299         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_10996, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000299 DASVVN010000000
VERSION     DASVVN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10996"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..517)
                     /locus_tag="VF686_04775"
     CDS             complement(20..517)
                     /locus_tag="VF686_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8661160.1"
                     /translation="MSTPSDPAFDIPAYRRALGGFATGVCVVTAHTGEGPLGITINSF
                     TSVSLNPPLVLWCLDRASDRHAAFASADRFAVHMLPAEDREMSDRFAWGVCRLSSDEF
                     DSSSVEPPRLKNALTRLDCVATDRIIMGDHLTIVGAVQSFETAPGPALVYYRGRYGVA
                     PEPGT"
     gene            634..1536
                     /locus_tag="VF686_04780"
     CDS             634..1536
                     /locus_tag="VF686_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX8661161.1"
                     /translation="MSMSDRPILIKKVKKVIGGGHHGGAWKVAYADFVTAMMAFFLLM
                     WLINTTDPEQKRGIAEYFAPASVSQTTSGSGGILGGTSLGDDGAKGSGSQSVISELAP
                     EAPADAPPEAGQSQNLAAASEEALREEITRREAFEFSSAAASLRQAMQSMPELAELSK
                     QLIIDQTPEGLRIQLVDQEGRSMFEQNSARPNPRAQLLLRTISRVITQLPNRISVTGH
                     TSAAPGSSRATGPNDWTLSSARANASRLILQAAGVDPDRVYQVSGKAGSDPLYPDDPT
                     LAGNRRIAIVLLRESPVLPRDTSL"
     gene            1660..2442
                     /locus_tag="VF686_04785"
     CDS             1660..2442
                     /locus_tag="VF686_04785"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX8661162.1"
                     /translation="MTFVPQRQLRFYMTSVAPCPYLPGQTERKVFANLPFSEGAHVND
                     ELTQAGFRRSQNIAYRPACEACDACVSVRLPVPEFAFSRSQRRVLKRNADLSRDLVEA
                     EATTEQFDLLRRYLNGRHPGGGMSAMTWLDYVAMVEDTAVRTHLIEYRMPSSDGGPGD
                     LVGVCLTDLLGDGLSMVYSFFEPEMEARSLGRFAILDHVRQAAAVNLPFVYLGYWVRG
                     SRKMDYKADFRPMEQLTRLGWERLDDQPAAGTEVSQGEGSGA"
     gene            complement(2391..>2619)
                     /locus_tag="VF686_04790"
     CDS             complement(2391..>2619)
                     /locus_tag="VF686_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661163.1"
                     /translation="EGRSSVKGPDELQQLFLDNQPDGVGLEGLFASHPPIQKRIDALV
                     KFGGGRDPGPWDGEAYAPEPSPWETSVPAAG"
BASE COUNT          429 a          897 c          886 g          407 t
ORIGIN      
        1 aggccggcga aaccgatttt catgtcccag gctccggagc gacgccatag cggccgcgat
       61 aatagacaag cgccgggccg ggtgcggtct caaagctctg caccgcgccg acgatggtca
      121 ggtgatcgcc catgatgatc cggtcggtcg cgacacagtc cagccgcgtc agggcgttct
      181 tcaggcgcgg cggctcgacg ctggaactgt cgaactcatc cgacgacagg cggcagacgc
      241 cccaggcgaa gcggtccgac atctcgcggt cctcggccgg cagcatatgg acggcgaacc
      301 ggtcggcgga tgcgaaggcc gcgtgcctgt cggaggcgcg atccagacac cacagcacca
      361 acggcgggtt cagcgacacc gaggtgaagg aattgatcgt gatcccgagg gggccctccc
      421 ccgtatgcgc ggtcaccacg cagacgccgg tcgcgaaacc gcccagcgcg cggcggtagg
      481 ccggaatgtc gaacgcggga tcggagggcg tgctcacacc ccgacgacta ggcgacggcc
      541 cggtccggag caagcccggc cggtatggga aacgcggtct taaccttgcc gcgtccatcg
      601 tcggcgtcag ttcgactgtc gaggccgcgt cccatgtcca tgagcgatcg tccgatcctc
      661 atcaagaagg tcaagaaggt catcggcggc ggccaccatg gcggcgcgtg gaaggtggcc
      721 tatgccgact tcgtcacggc gatgatggcc ttcttcctgc tgatgtggct gatcaatacg
      781 accgatccgg aacagaagcg cggcatcgcc gaatatttcg cgcctgccag cgtgtcgcag
      841 acgacgtcgg gttcgggcgg cattctgggc ggaacctcgc tgggtgacga cggcgccaag
      901 ggctcgggtt cgcagtcagt gatcagcgaa ctggcgccgg aggcgcccgc ggacgcgccg
      961 cccgaagccg ggcagagcca gaacctggcc gccgccagcg aagaggccct gcgcgaagag
     1021 atcacccggc gcgaggcctt cgaattctcg tcagccgctg catcgctccg tcaggccatg
     1081 caatcgatgc ccgagctggc cgagctgtcg aaacagctga tcatcgacca gacccccgaa
     1141 ggcctccgca tccagctggt cgatcaggaa ggccgttcga tgttcgaaca gaactcggcc
     1201 cggcccaatc cgcgcgccca gctgctgctg cgcaccatct ccagggtcat cacccagctg
     1261 ccgaaccgca tctcagtgac gggccacacc tcggccgcgc cgggatcgag ccgtgcgacc
     1321 ggcccgaacg actggaccct gtcttcggcc cgcgccaatg cctcgcggct catcctgcag
     1381 gccgcgggcg tcgatccgga ccgcgtctat caggtgtctg gcaaggcggg gtccgatccg
     1441 ctgtatccgg atgatccgac gctggccggc aaccgccgca tcgccatcgt gctgctgcgc
     1501 gagtccccgg tcctgccgcg ggacaccagc ctgtgacccg cggccccgca cgcctgtgtg
     1561 gggcgaaggg gcttgccccg cgaggcgacg cgggcgcaaa tcgtcccatg acgaaacggc
     1621 tgccctactg tcggcgaaat cggatgcgga tgtagagccg tgaccttcgt tccgcagcgc
     1681 cagcttcgct tctacatgac ctcggtggcc ccctgcccct atctgcccgg gcagaccgag
     1741 cggaaggtgt tcgccaacct gcccttcagc gagggcgcgc acgtcaacga cgagctgacc
     1801 caggccgggt tccgccgcag ccagaacatc gcctaccgcc cggcctgcga ggcgtgcgac
     1861 gcctgcgtct cggtgcggtt gcccgtgccg gagttcgcct tctcgcgctc ccagcggcgg
     1921 gtgctgaagc ggaacgccga cctgtcgcgc gatctggtcg aggccgaggc gacgacggag
     1981 cagttcgacc tgctgcgtcg ctatctgaac ggccgccatc ccggcggcgg aatgagcgcc
     2041 atgacatggc tcgactatgt cgccatggtc gaggatacgg cggtccgcac ccatctgatc
     2101 gagtaccgca tgccgtcgtc cgacggcggg cccggggatc tggtcggggt ctgcctgacc
     2161 gacctgctcg gggacgggtt gtcgatggtc tacagcttct tcgagccgga gatggaggcc
     2221 cgcagcctgg gccggttcgc gatactggac catgtccgtc aggcggcggc ggtgaacctg
     2281 cccttcgtct atctgggcta ctgggtccgg ggctcgcgca agatggacta caaggccgac
     2341 ttccggccga tggaacagct gactcggctg ggctgggagc ggctggacga tcagcctgcg
     2401 gccggcaccg aggtttccca aggcgagggc tccggcgcgt aagcttcgcc gtcccaaggg
     2461 ccggggtccc gccctccccc gaacttcacc agggcgtcga tccgcttctg gatcggcggg
     2521 tggctggcga acaggccctc gaggccgacg ccgtccggct ggttgtcgag gaacagctgc
     2581 tggagttcgt cgggcccctt caccgatgac cggccctcg
//
LOCUS       DASVVN010000300         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_222416,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000300 DASVVN010000000
VERSION     DASVVN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222416"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VF686_04795"
     CDS             complement(<1..1127)
                     /locus_tag="VF686_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8661164.1"
                     /translation="MKIDRRQALTGAAMLGLGSISGAGPALAQTQRVRRGAGTLSATS
                     DDVVAFGEGLALMKRRRDDRSWARQNTIHSRNGQHNNGLFLPWHRLQLLHLERIIAGL
                     TGHAGFAMPYWDAQEHRTLPSWITDEDALLYERQRARGVDVLDFNAARWARSPYMSRL
                     TSDGFETFAGKLPTGAGMVEGYGHNYIHELVGGLMKRLTTAASDPIFWLHHCNVDRVW
                     ATWHARQPASVYPDDWTARTLNGFVGGQGEDTGDWRVAGVLETRPLGYGYDQLYPFPV
                     FNVPEVGPPGATRRVPLGSVVYPMRAEGGAGALSAALPAEALARMRAADDTLMIAGTG
                     SVAYAATEALLDRSIEIRMSCAGRRISLGSSPTFLHLREGD"
     gene            complement(1170..1703)
                     /locus_tag="VF686_04800"
     CDS             complement(1170..1703)
                     /locus_tag="VF686_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661165.1"
                     /translation="MTDDTTGKDADQPEALQPFPVLGADSPFPPLYESLPPPPRQLAE
                     DPPSDWIESADAHDLPTRENALAALGRFAFPPAPPLDDEGIAARDRRWTSRAIFTVAA
                     FLLVFNAVSPLNWSRQQAPGWVQGTVEQLSEVWVAQLAVFGVDMPRQGLRDMWTAARQ
                     ARFIGQAPARDTGSTPG"
BASE COUNT          257 a          655 c          580 g          249 t
ORIGIN      
        1 tggtctccct cccgcaggtg caggaaggtc ggcgacgagc ccagcgatat tcgtcgcccg
       61 gcgcagctca tgcggatctc gatcgaccga tccagcagcg cctcggtcgc ggcgtacgcc
      121 accgaccccg tgcccgcgat catcagcgtg tcgtccgccg cccgcatccg cgccaacgcc
      181 tcggcgggca gggctgccga cagggcgccc gccccaccct cggcccgcat tggatagacg
      241 acgctcccca gcggtacgcg ccgcgtcgcg cccggcggcc ccacctcggg cacattgaac
      301 accgggaacg gatacagctg gtcatagcca tagccgagcg gccgggtctc cagcacgccg
      361 gccacccgcc agtcgcccgt atcctcgccc tgcccgccga cgaagccgtt cagcgtccgc
      421 gccgtccagt cgtcgggata gacgctggcc ggctgacgcg cgtgccaggt cgcccagacc
      481 cggtcgacgt tgcagtggtg cagccagaag atgggatcgc tcgctgccgt cgtcagccgc
      541 ttcatcagcc cgccgaccag ctcgtggatg tagttgtggc catagccctc gaccatgccc
      601 gccccggtcg gcagctttcc cgcgaaggtc tcgaagccgt ccgaagtcag ccgggacata
      661 tagggggacc gggcccagcg ggcggcgttg aagtccagca catcaacacc gcgcgcccgc
      721 tgccgctcgt acagcagcgc gtcctcgtcc gtgatccacg aaggcagggt tcggtgctcc
      781 tgcgcgtccc agtagggcat ggcgaaaccg gcgtggcccg tcaggcccgc gatgatccgc
      841 tccagatgca gcagttgcag ccggtgccat ggcaggaaca ggccgttgtt gtgctggccg
      901 ttgcgcgaat ggatcgtgtt ctgccgggcc caggaccggt cgtcccgccg ccgcttcatc
      961 agggccagcc cctcgccgaa ggccacgacg tcgtcgctgg tcgccgacag ggtcccggcg
     1021 cctcggcgca cccgctgcgt ctgggccaat gccggtccgg cgcccgaaat cgagccgagg
     1081 cccagcatgg cggcgccggt cagcgcctgt cgtctgtcga ttttcaccca tgcccccgca
     1141 cgtcttcgga aggcgaaggt tcaccaatct cagcccggtg tcgaccccgt gtcgcgggcc
     1201 ggcgcctggc cgatgaaccg cgcctgccgc gcggccgtcc acatatcgcg caggccctga
     1261 cgcggcatgt cgaccccgaa cacggccagc tgcgcgaccc agacctcgga cagctgctcc
     1321 accgtcccct gcacccagcc cggcgcctgc tgccgcgacc agttcagcgg cgacacggcg
     1381 ttgaacacca gcaggaaggc cgcgacggtg aagatcgccc ggctggtcca gcgccggtcc
     1441 cgcgcggcga tgccctcgtc gtccagcggc ggcgcgggcg ggaaggcgaa ccggcccagc
     1501 gcggccagcg cattctcgcg cgtcggcagg tcgtgggcgt cggcgctctc gatccagtcg
     1561 ctgggcggat cctcggccag ctgccggggc ggcggcggca gactctcgta cagcggcggg
     1621 aacggcgagt ccgcacccag aaccgggaac ggctgcagcg cctcgggctg gtctgcgtcc
     1681 ttgcctgtcg tatcgtcggt catcgcgggt ccaacgcctc agaactggaa atagatgaag
     1741 g
//
LOCUS       DASVVN010000301         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_483503,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000301 DASVVN010000000
VERSION     DASVVN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483503"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1512)
                     /locus_tag="VF686_04805"
     CDS             complement(106..1512)
                     /locus_tag="VF686_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8661166.1"
                     /translation="MTKATLKPLNQQAIVITGATSGIGLATARRAARAGACVFLIARG
                     EKDLKALCEELQGAGARAAWAVADVADHAALAEAAEKCVRLFGGFDTWVNNAGVSIFG
                     SIGTTKLEDQRRLFETNYWGVVNGSMIAADHLRDRPGGGAIVNVGSILSDAPIPIQGV
                     YSASKHAVKGFTNALRMELLREQAPIALSLVKPSAIDTPYNKHARNLTGQAMQNPQPV
                     YATHVVADTILYCASHPIREITVGGGGRLIASFYSALPGLAEPLFARFAPSLMRDRGS
                     AYQPFDDGLYDPTDDGLDEEVHYPMVRQFSALAEVRKHPGITGGVVAILAGVGLAALL
                     LNQRSGPTRYERIRGRIDPRGWVDTHALRDRFGGLAETVRHGFADATERASDMSDEAR
                     HRGERILHGARHSSRKALKKHGKTARRYAHDAGAYAKDHAREGGAALALLTVAAAIGA
                     AAMDARRPDSRLRSLGKF"
     gene            1808..>1963
                     /locus_tag="VF686_04810"
     CDS             1808..>1963
                     /locus_tag="VF686_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Hsp33"
                     /protein_id="HEX8661167.1"
                     /translation="MTDHAHTPANPADDLAAAFQIEGWPVRGRLVRLGDTIDAILSAH
                     DYPEPVAA"
BASE COUNT          301 a          639 c          695 g          328 t
ORIGIN      
        1 aaacatagtc cacagagtcc actccgttca cattgaaagc cggccgcccc gcaagggacg
       61 accggcgctc agtgtgtcag gcctctacgt cagaatcgga cgtagtcaga attttccgag
      121 gctgcgcagg cggctgtcgg gacggcgggc gtccatggcg gccgcgccga tggcggcggc
      181 gacggtcagg agggccaagg cggcgccgcc ttcgcgggcg tgatccttgg cgtaggcgcc
      241 ggcgtcatgg gcgtatcggc gcgccgtctt gccgtgcttc ttcaacgcct tgcgcgagga
      301 atgtcgggct ccgtgcagga tccgctcacc gcgatggcgg gcctcgtcgc tcatgtcgga
      361 ggcgcgttcg gtggcgtcgg cgaagccgtg acggaccgtc tcggccaaac ccccgaagcg
      421 gtcgcgcagg gcgtgcgtat cgacccagcc gcgcgggtcg atgcggccgc ggatgcgctc
      481 atagcgggtc gggcccgagc gctggttcag cagcagggcg gcaaggccga cgccggcgag
      541 aatggcgacg acgccgcccg tgatgccagg atgtttgcga acttcggcga gggcggagaa
      601 ctggcgaacc atgggatagt gaacctcttc atcgaggccg tcgtcggtgg ggtcgtagag
      661 cccgtcgtca aacggctgat aagcggaacc tctgtcccgc atgaggctag gagcgaaccg
      721 ggcgaaaaga ggttccgcca gaccgggcag ggcggaatag aagctggcga tcagccgccc
      781 gccgccgccg acggtgatct cgcggatcgg atgactggcg caatacagga tggtgtcggc
      841 cacgacgtgg gtcgcataga ccggctgggg gttctgcatc gcctggccgg tcaggttgcg
      901 ggcgtgcttg ttgtacggcg tgtcgatggc cgacggcttg accagcgaca gagcgatcgg
      961 cgcctgttcg cgcagaagct ccatgcgcag ggcgttggtg aatcccttca ccgcatgttt
     1021 ggaggcggaa tagaccccct ggatcgggat cggcgcgtcg gacaggatcg atccgacatt
     1081 gacgatggcc ccgcccccgg ggcggtcgcg caggtggtcg gcggcgatca tcgagccgtt
     1141 gaccacgccc cagtagttgg tctcgaagag tcggcgctga tcctcaagct tcgtcgtgcc
     1201 gatactgccg aagatcgaga cgccggcgtt gttgacccag gtgtcgaagc cgccgaacag
     1261 ccgcacgcac ttttccgcgg cctcggcgag ggcggcgtga tcggccacgt cggcgacggc
     1321 ccaggccgcc cgggcgccgg ccccctgaag ctcctcgcac agggccttca ggtctttctc
     1381 gccgcgcgcg atcaggaaga cgcaggcccc ggcccgggcc gcccgccgcg ccgtggccag
     1441 accgatgccc gaggtggcgc cagtgatgac gatggcttgc tggttcaggg gcttgagggt
     1501 cgctttggtc atggtcgcgg actcatcctt ggggcgtctg acctgtatcc ccgtcattcc
     1561 cggggcgggc aagctcggct cctgcaattc agtcttgtcg tcatcccggc caatgccggg
     1621 acctagcggc gccgagcata gcgaaggcga tgttcaacgc ttccgcgctc ccgacagttt
     1681 cacgctgtcg cgtgccgctg ggtcccgggg tcgctgcgcg ccccggcaag acaggagcgg
     1741 cgccttatca tcgctgcatc ggttcactat cttggcgggc tcccgtgttt ctgaccaagg
     1801 atcgtccgtg accgatcacg cgcatacgcc cgccaatccc gccgacgatc tggccgccgc
     1861 cttccagatc gagggctggc ccgtgcgcgg gcggctggtg cggctgggcg acaccatcga
     1921 cgccatcctg tcggcccatg actacccgga accggtcgcc gcc
//
LOCUS       DASVVN010000302         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_11205, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000302 DASVVN010000000
VERSION     DASVVN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11205"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF686_04815"
     CDS             <1..455
                     /locus_tag="VF686_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661168.1"
                     /translation="IELARLLAPGMVDRGYGRILNVASLAGLMPATGGDTLYGPIKSF
                     LIKASQGLHLELRGTGVNVTALCPGYTLTEFHDANGTREQVSSAYPAWMWQTAEHVAR
                     VGYAACEANRPKVTPGVMNNLLAGLAKHLPDRVALGMVGGHAKRLKRI"
     gene            complement(452..799)
                     /locus_tag="VF686_04820"
     CDS             complement(452..799)
                     /locus_tag="VF686_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661169.1"
                     /translation="MRNVMIVDGADNATFSVFQTTEEEFAILFPASGQDIAFIEEVVE
                     RLGDAATGAALNPMWERPILRRDAMGLHGLLIYDGEGRRPFYPASGREMDFEQSAVNT
                     AQRELFARKRSEA"
     gene            complement(802..1500)
                     /locus_tag="VF686_04825"
     CDS             complement(802..1500)
                     /locus_tag="VF686_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX8661170.1"
                     /translation="MDAVDLGVNLFVALFALVDPIGNIPIFAAATAGASVRQRLSVSF
                     LICLFAAAFLAFFFFTGLGLLQFFGISLAAFRIAGGILLLLLGLDMTRGDFLKSFADN
                     DTDSDPLDVRGYARRRFQRLVVPFAMPLMIGPGAISTIIIQAGEASKIGYQGQVAGMV
                     AIVAACIATFLCFALTGPISRILGEIGMSIIVRVLGLILCALAIQFILLGLSEAIPGM
                     ISGAVTTPYPTGGH"
     gene            1596..>2190
                     /gene="pyrF"
                     /locus_tag="VF686_04830"
     CDS             1596..>2190
                     /gene="pyrF"
                     /locus_tag="VF686_04830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX8661171.1"
                     /translation="MTATRFDPRLIVGLDLPSVDAARAMVDRLGDSVSFYKIGLTLLA
                     RPGGVAFAHTLRAAGKQVFQDWKLHDIGAQVEGAARAVAEGGCDLLTVHAEPPAMRGA
                     VRGRDATGAGTKLLAVTVLTSLSDEDLREIGYGSGAAELVERRVRQALDCGVDGVVSS
                     PLEAARVREIANAAGRPDFLIVTPGVRPEGADKGDQQR"
BASE COUNT          382 a          763 c          735 g          310 t
ORIGIN      
        1 cgattgagct ggcccgactg ctggcgcccg gcatggtcga tcgcggctac ggccgcatcc
       61 tgaacgtcgc gtctctggcg ggcctgatgc cggccaccgg cggcgacacc ctgtacggcc
      121 cgatcaagag cttcctgatc aaggcctcgc aggggctgca cctcgagctg cgcgggaccg
      181 gcgtcaacgt caccgccctt tgccccggct acaccctgac cgaattccac gacgccaacg
      241 gcacgcgtga acaggtctcg tcggcctatc ccgcctggat gtggcagacg gcggagcatg
      301 tggcgcgcgt cggctacgcc gcctgcgagg ccaaccggcc gaaggtgacg ccgggggtga
      361 tgaacaatct gctggccggg ctcgccaaac acctgccgga tcgggtggcg ctgggcatgg
      421 tcggcggcca tgcgaaacgg ctgaagcgga tctaagcctc gctccgcttc cgggcaaaga
      481 gctcgcgctg cgctgtattc accgcgctct gctcgaagtc catctccctg ccgcttgccg
      541 gatagaaagg ccgtctccct tcaccgtcgt agatgagcag accgtgtagg cccatggcgt
      601 cgcgtctaag gatcggccgc tcccacatgg gattcagggc agccccggtc gctgcatcac
      661 caagcctttc aaccacctct tcgatgaacg caatgtcttg gccggacgcc ggaaagagga
      721 tggcgaactc ctcttcggtg gtctgaaaca cgctgaatgt tgcgttgtca gcgccatcaa
      781 cgatcatgac attgcgcacg atcagtgccc gcccgtcggg tacggcgtcg tcaccgcccc
      841 cgagatcatg ccgggaatgg cttccgacag gcccagcagg atgaactgga tcgcaagggc
      901 gcacaggatc aggcccagaa cgcgcacgat gatcgacatg ccgatctcgc cgaggatgcg
      961 gctgatgggt cccgtcaggg cgaagcacag gaaggtggcg atacaggcgg cgacgatggc
     1021 gaccatgccc gcgacctggc cctgatagcc gatcttcgag gcctcgccgg cctggatgat
     1081 gatggtcgag atggcgcccg gtccgatcat caggggcatg gcgaagggca cgaccagccg
     1141 ctggaaccgc cgccgcgcat agccgcgcac gtccagcgga tcgctgtccg tgtcgttgtc
     1201 cgcgaacgac ttgaggaagt ccccgcgcgt catgtcgagg cccagcagca gcagcaggat
     1261 gccgcccgcg atccggaacg cggccagcga gatgccgaag aactgcagca gccccagtcc
     1321 ggtgaagaag aagaatgcga gaaaggcggc cgcgaacagg cagatcagga acgacacaga
     1381 cagccgctgg cgcacgctgg cccccgccgt cgcggcggcg aagatcggga tgttgccgat
     1441 cgggtccacg agcgcgaaca gggccacgaa caggttgacg cccagatcga ccgcgtccat
     1501 gtgctgacct cgccctgcaa ttcacccaac cgcggcccat caaggctgac gcccgcgcgc
     1561 gcgtccagcc ctaacgggtc cagaggagcg acgccatgac tgcgacccga ttcgaccccc
     1621 gcctgatcgt cggcctggac ctgccctccg tcgacgcggc ccgcgccatg gtggaccggc
     1681 tgggcgactc ggtcagcttc tacaagatcg gcctgaccct tctggcccgc cccggcggcg
     1741 tcgccttcgc ccacaccctc cgcgccgcgg gcaagcaggt gttccaggac tggaagctgc
     1801 acgacatcgg cgcccaggtc gaaggcgcgg cccgcgcggt ggccgagggc ggctgcgacc
     1861 tgctgaccgt ccacgccgaa cccccggcga tgcgcggcgc ggtcagggga cgcgacgcga
     1921 ccggagcggg cacgaagctg ctggccgtca ccgtcctgac cagcctgtcg gacgaggatc
     1981 tgcgcgagat cggctacggc tccggcgcgg ccgagctggt cgagcggcgg gtgcgccagg
     2041 ctctggactg cggcgtcgac ggcgtggtct caagccccct ggaagccgcc cgggtgcgcg
     2101 agatcgccaa cgccgccggc cggcccgact tcctgatcgt gacccccggc gtccggcccg
     2161 agggcgcgga caagggcgat cagcagcgcg
//
LOCUS       DASVVN010000303         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_11209, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000303 DASVVN010000000
VERSION     DASVVN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11209"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF686_04835"
     CDS             <1..477
                     /locus_tag="VF686_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8661172.1"
                     /translation="AFEESNPLARAWFEGNAMDGQVPADPWTLVKDSDRPDYRMAARP
                     DWAEAATETASEAEAAVLARTGAGPVRDAVDGRIVAGVRARSLRIIDSPSQVGGWPEL
                     AAGKPWVDGDGDGMPDDWETANGLNPAEAADGTADRNGDGWTNLEDWLDSLARPAD"
     gene            complement(653..1396)
                     /gene="kduD"
                     /locus_tag="VF686_04840"
     CDS             complement(653..1396)
                     /gene="kduD"
                     /locus_tag="VF686_04840"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEX8661173.1"
                     /translation="MNPFDLSGRTALVTGANTGLGQAIAVGLAGAGADIVAVGRSAPT
                     ETEALVAGLGRSFKAISADLGSTAPIADVVAQAGPVDILVNNAGIIRRADSLDFSEAD
                     WDAVMDTNLKTAFFLSQAVARTWVADGRGGKIINICSMLSFQGGIRVPSYTASKSGLA
                     GLTRLLACEWASKGINVNGIAPGYFATNNTEALRADETRNRDILARIPAGHWGQPSDL
                     AGAAVFLSSDAASYVHGTVLAVDGGWLAR"
     gene            complement(1393..2226)
                     /gene="kduI"
                     /locus_tag="VF686_04845"
     CDS             complement(1393..2226)
                     /gene="kduI"
                     /locus_tag="VF686_04845"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX8661174.1"
                     /translation="MYSKTYHATHPDMMDGVTNDQLRERYLIDGLFEDGQIRLNYTHY
                     ERMVIGGVQPGSTTVRLPDQTEPASAAGHPFLERRELGVVNVGAATGTVTVDGQAYTL
                     EPNDGLYIAMGSKDVTFAGDTRFYLASTPAHVRYETKHILRGQAVALERGALETSNER
                     TIYQYIVPATCKSCQLLLGVTVLKTGSVWNTMPPHLHDRRSEAYFYFGLSENDRVFHF
                     MGEPDAQRHIVVADGEAVVSPPWSIHMGAGTSNYTFIWVMGGENLDYTDMNVLDICQL
                     K"
     gene            2397..>2759
                     /locus_tag="VF686_04850"
     CDS             2397..>2759
                     /locus_tag="VF686_04850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8661175.1"
                     /translation="MDLKNRSVVITGGSRGLGLGVVEALAGQGALVTVVARASEALDA
                     VAALPGVTAVAADIRDEEAAGRIVTDVRPDILILNAGAKPPMARMDEISWADFTATWE
                     HDVRAGLYWLQAALNLPLR"
BASE COUNT          448 a          871 c          990 g          450 t
ORIGIN      
        1 gcgtttgagg agtccaatcc cctcgcccgg gcgtggttcg aaggcaatgc gatggacggg
       61 caggtcccgg cggacccgtg gacgctggtc aaggactccg accgtccgga ctaccggatg
      121 gcggcgcggc ccgactgggc ggaagccgcg accgaaacgg cgtcggaagc cgaggcggcg
      181 gtgctggccc ggaccggggc gggtccggtc cgcgacgccg tggacgggcg gattgtcgcc
      241 ggagtccggg cgcggtcgct gcgcatcatc gacagcccgt cacaggtcgg aggctggccc
      301 gagctggcgg ccggaaagcc gtgggtcgac ggcgacggcg acggcatgcc cgacgactgg
      361 gagacggcga acggcctgaa cccggccgag gccgccgacg gaacggccga ccggaacggc
      421 gacggctgga ccaatctgga agactggttg gacagtttgg cccggcccgc agactgaccg
      481 cagccctgct tcagggccgt ttcgaaatgt caagtgtccg cgaaaattcg tgggacagtt
      541 tcggaggaca attcgcccgc caaacgtttt gatagacctg tcaaagaccg ggccgcacgc
      601 gaccctcgac aatgcaacac gcgcctgcgt caaaaacggt cgtagggcgg cgctaccgcg
      661 ccagccagcc gccgtcgacg gccagcaccg tgccgtggac atagctggcg gcgtccgacg
      721 acaggaagac cgccgcgccc gccagatccg agggctggcc ccagtgtccg gccggaatgc
      781 gggcgaggat gtcgcggttg cgcgtctcgt cggcgcgcag ggcctcggtg ttgttggtcg
      841 cgaaatagcc gggtgcgatg ccgttgacgt tgatgccctt cgacgcccac tcgcaggcca
      901 gcagccgcgt caggccggcg aggccgcttt tggaggcggt gtaggacggc acgcggatgc
      961 cgccctggaa ggacagcatc gaacagatgt tgatgatctt gccgccccga ccgtccgcca
     1021 cccaggtccg ggccacggcc tggctgagga agaaggcggt cttcagattg gtgtccatga
     1081 ccgcgtccca gtcggcctcg ctgaagtcga gactgtcggc gcggcggatg atgccggcgt
     1141 tgttgaccag aatgtcgacg ggacccgcct gggcgaccac gtccgcgatg ggcgcggtcg
     1201 agccgaggtc ggcggagatc gccttgaacg atcgacccag acccgcgacc agcgcctcgg
     1261 tctcggtcgg cgccgaacgg ccgacggcga cgatgtcggc ccccgctccg gccaggccga
     1321 ccgcgatggc ctggcccagt ccggtgttgg cgccggtgac gagggccgtg cggccgctga
     1381 ggtcgaaggg gttcatttca gctggcagat gtcgaggacg ttcatgtcgg tgtagtcgag
     1441 gttctcgccg cccatgaccc agatgaaggt gtagttggac gtgcccgcgc ccatgtggat
     1501 cgaccatggc ggcgacacca ccgcctcgcc gtcggcgacc acgatgtgcc gctgcgcgtc
     1561 cggctcgccc atgaagtgga agacgcggtc gttttcggac aggccgaagt agaaataggc
     1621 ctcggagcgg cggtcgtgca ggtgcggcgg catggtgttc cagacgctgc ccgtcttgag
     1681 gacggtcacg cccagcagca gctggcagga cttgcaggtg gcgggaacga tgtattggta
     1741 gatggtccgt tcgttcgagg tctccagcgc gccccgctcc agcgccacgg cctgcccgcg
     1801 caggatgtgt ttggtctcgt agcggacgtg cgcgggcgtc gaggccagat agaaacgggt
     1861 gtcgccggcg aaggtgacat ccttcgaacc catggcgatg tacaggccgt cgttgggctc
     1921 cagcgtatag gcctggccgt ccacggtgac cgtgcccgtg gcggcaccga cgttgaccac
     1981 gcccagctcg cgccgctcga ggaacggatg acccgcggcc gaggcgggct cggtctggtc
     2041 gggcagtctg acggtcgtcg accccggctg gacgccgccg atgaccatgc gctcatagtg
     2101 ggtgtagttc aggcggatct gtccgtcctc gaacagcccg tcgatcagat agcgctcgcg
     2161 caactggtcg ttggtgaccc cgtccatcat gtccgggtgg gtggcgtgat aggtcttgct
     2221 gtacatgggg aacaccttgt ccgggtcgga gcgtggcgtc taggtaaccg gtgtcataga
     2281 tggctgcaag gttcctgaga cccggaagga ttgttcgtcg gcacgccgcc tccatcttgt
     2341 gactcttttt tggcccgccg ttcgtcttgc ctccatacga acatggagcc ggacagatgg
     2401 acctcaaaaa cagaagcgtg gtgatcactg gcggaagccg tggcctcgga ctcggagtgg
     2461 ttgaagcctt ggctggccaa ggcgccttgg tgaccgtcgt cgcgcgcgcc tccgaggcgc
     2521 tggacgcagt tgctgcgctg ccgggcgtca ccgcggtcgc cgccgacatc cgggatgaag
     2581 aggccgccgg ccgcatcgtg actgatgtcc ggcctgacat cctgatcctg aatgcggggg
     2641 ccaaaccgcc catggcgcga atggacgaga tcagctgggc cgatttcacc gcgacctggg
     2701 agcacgacgt tcgcgcagga ctgtactggc tacaggccgc cctgaacctg cccctccgt
//
LOCUS       DASVVN010000304         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_11226, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000304 DASVVN010000000
VERSION     DASVVN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11226"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2262)
                     /locus_tag="VF686_04855"
     CDS             complement(<1..2262)
                     /locus_tag="VF686_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8661176.1"
                     /translation="MSDRGAGQAPSRVVAVLGPTNTGKTHLAVERMLGHASGMIGLPL
                     RLLAREIYDRIVKRRGAAAVALVTGEEKIVPPRAHYFVCTVEAMPMERSVEFLAVDEI
                     QLVADPERGHVFTQRLLHARGRFETMFLGAGTMAPLVRSLVPDVEIVSRERLSTLSYA
                     GSKKLTRLPARSAIVAFSTEQVYAIAELIRRQRGGAAVVMGSLSPRTRNAQVELFQSG
                     EVDFLVATDAIGMGLNMEVDHVAFAGLRKFDGRRTRWLHAHEIGQIAGRAGRHLRDGT
                     FGVTGESLELDPDLVEQVVEHRFDPVEAAEWRNARLDFDTLPDLLRSLVVSPGRAGLS
                     LTQPALDETLLRRLIKDEEVARIGRSRGAIMRLWEACQLPDFQKTTLDEHGRLSKDIF
                     QALTGKRGRLTEDWIAPRFAELDRDDGQIDQLSARLSGVRTLSYIANRPDWLDQAQGW
                     RDRTKALEERLSDVLHERLTARFVDRRTTALMRALNVREDAVADVDEEGAVIVEGHAV
                     GRLTGVDFQIDKGSSALENRALRHAARQAVTPEIARRLGRLAADADEAFGVMSDGAVL
                     WRGVQTGQVVGGDWFAPRVRLLGELGPVAARERAQRRLEAWLAAEAGRALKPLKRLKT
                     AVESGALKGLPRGLAFRLLEAGGIVDRREVDRDIAALSQVERRTLRTFGVRMGAHSVW
                     LPALLKARPRALAQAFTAGESFRPGGQTLTLLPDPAPTSRHLSALGLRAVGRFAASVD
                     LLEGMTERRAKGKG"
     gene            2561..2637
                     /locus_tag="VF686_04860"
     tRNA            2561..2637
                     /locus_tag="VF686_04860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2595..2597,aa:Arg,seq:cct)
     gene            2836..3588
                     /locus_tag="VF686_04865"
     CDS             2836..3588
                     /locus_tag="VF686_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661177.1"
                     /translation="MTDLKISSRVFTAWRSRETLVLAAELTAGVTAIAFAASAGLNLF
                     LFSHWGLNYLQIASPADVVMGGIEVFLRLIPFLALAAGTSWLGRMLPMRGIGQALLGA
                     FCLVAILGSFLLVARDLPNEGSSFGWFRPWRDYVVFVAVAAFGGLAAPLAERDGVAVS
                     FNLLLGFTCFFVALVGVEIGVSRGYWPNEAVPRWAAGVTVEPCDEPQLLWSGSNAVVL
                     ECPGYRVVVRDAGALSIRVPYRPRWERSYPRP"
     gene            3691..3876
                     /locus_tag="VF686_04870"
     CDS             3691..3876
                     /locus_tag="VF686_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661178.1"
                     /translation="MQDNNRRFADDMAQLDAELGRAIEQRASLSAAEQDRFLGEDSSL
                     LRGLLAGMTFAGAPGRR"
BASE COUNT          599 a         1292 c         1333 g          702 t
ORIGIN      
        1 gcctttcccc ttcgcgcgcc gctcggtcat tccctcaagc agatccaccg atgcggcgaa
       61 ccggccgacc gcacgcaggc cgagggcgga caggtggcgc gaggtcggag cggggtcggg
      121 cagcagggtc agggtctgac cgccgggccg gaaggattcg ccggccgtga aagcctgcgc
      181 caatgcgcgg ggccgcgcct tcagcagggc cggcagccag accgaatggg cgcccatgcg
      241 gacgccgaag gttctgagcg tgcggcgttc cacctgactg agggcggcaa tgtcgcggtc
      301 gacctcgcgc cggtcgacga ttccgccggc ctccagcagg cggaaggcca ggccgcgcgg
      361 aagacctttc agggcgccgc tctcgaccgc cgtcttgagc cgcttcagcg gtttaagcgc
      421 ccggcccgcc tcggccgcca gccaggcttc gagccgccgc tgcgcccgct cgcgcgcggc
      481 gacgggcccc agctcgccca gcaaccggac gcgcggcgcg aaccagtcgc cgccgacgac
      541 ctgaccggtc tggacgccac gccacagcac cgcgccgtcg gacatgacgc cgaaggcctc
      601 gtccgcgtcc gccgccagcc gtcccagccg gcgcgcgatc tcgggcgtca ccgcctgccg
      661 cgccgcgtgc cgcagggccc ggttctccag cgcggacgag cccttgtcga tctggaagtc
      721 gacgcccgtc agccgaccga cagcatggcc ctcaacgatc acggcgccct cttcgtcgac
      781 atccgcgacc gcgtcctccc gcacgttcag ggcccgcatc agggcggtgg tgcgccggtc
      841 gacgaaccgc gcggtcagcc gctcgtgcag cacgtcgctg agccgttcct ccagcgcctt
      901 ggtgcggtcg cgccagccct gggcctgatc cagccagtcg gggcggttgg cgatatagct
      961 gagggtgcgc acgccgctga gccgggccga cagctggtcg atctggccgt cgtcccggtc
     1021 gagttcggcg aagcggggcg cgatccagtc ctcggtcagc cgtccacgct tgccggtcag
     1081 ggcctggaag atgtccttgg acagccggcc gtgctcgtcc agcgtggtct tctggaagtc
     1141 gggcagctgg caagcctccc aaagccgcat gatggccccg cgcgagcggc cgatgcgcgc
     1201 cacctcctcg tccttgatca ggcggcgcag cagggtttcg tccagcgccg gctgggtcag
     1261 gctcaggcca gcgcggccgg gcgacaccac cagtgaacgc agcaggtcgg gcagggtgtc
     1321 gaaatccagc cgggcgttac gccactcggc cgcctcgacc gggtcgaagc gatgctcgac
     1381 cacctgttcg accaggtcgg ggtccagctc cagcgactcg cccgtgacgc cgaaggtgcc
     1441 gtcgcgcagg tggcggccgg cgcggccggc gatctggcct atctcgtggg cgtgcagcca
     1501 acgggtgcgg cggccgtcga acttccgcag gccggcaaag gcgacgtggt cgacctccat
     1561 gttcaggccc atgccgatgg cgtccgtggc gaccagaaag tcgacctcgc ccgactggaa
     1621 tagctcgacc tgggcgttgc gggtgcgggg gctcagactg cccatgacca cggcggcgcc
     1681 gccgcgctga cgccggatca gttcggctat ggcgtagacc tgttccgtgc tgaaggcgac
     1741 gatggcgctg cgggcgggca ggcgggtcag cttcttcgat cccgcatacg acagggtaga
     1801 cagccgctcg cgcgagacga tctccacatc ggggaccagc gaccggacca gcggggccat
     1861 ggtccccgcg cccaggaaca tggtctcgaa ccggccacgg gcgtgcaaca gccgctgggt
     1921 gaagacgtgg ccgcgctcgg ggtcggcgac cagttggatc tcgtcaacgg cgaggaattc
     1981 gaccgaccgc tccatcggca tggcctcgac ggtgcagacg aaatagtggg cccgcggcgg
     2041 gacgatcttc tcctcgccgg tgaccagcgc cacagccgcc gcgccgcgcc gtttgacaat
     2101 ccggtcgtag atctcgcgcg ccagcagccg cagcggcagg ccgatcatgc ctgaggcgtg
     2161 acccagcatc cgctcgaccg ccagatgggt ctttccggta ttcgtggggc ccagcaccgc
     2221 aacgacgcgc gatggggcct gtccggcgcc gcgatcactc atgatatgga aaaggtggcg
     2281 tctcccgtcc ggttcctcaa gcggcgccgt gacggcggga cgagttgtaa acagccaagg
     2341 ttcgaacggc ggaacagggg cgaaacgaat caggaccgaa tcgccgacac ggcccgattc
     2401 ggggtttgtt ccctacaaga tattgtatct taacgcatct taatcacaag acggaacgcg
     2461 gttctggtgg agcgaggccg gccagtccgg cgcgcggccg cgggaaaacg catcggaccg
     2521 cttccccgag gccgtcccag acgctaggtc agcgctgcgt ggccccgtag ctcagccgga
     2581 tagagcaagg ctttcctaaa gcagaggtcg ggggttcgag tccctccggg gtcgccaacg
     2641 ttttcaatca gttatgtagt acggttttgt ccgatgtgtt ctccgggaca cgacagggac
     2701 acattcggca catgttagcc gacgtggaga ccagcgttct ccgacctgtt tttcgtggcg
     2761 tggctgtgct ccattgcaac tcccaagtga cgcaggccat cggcgcttgg ttgcgaggtg
     2821 ctttgggggg gtgctgtgac ggatctgaag atcagttccc gtgtcttcac ggcttggcgc
     2881 tctcgcgaga cgcttgtctt ggcggccgaa ctcacagcgg gtgttacggc tatcgccttt
     2941 gcggcctcag caggcctgaa cctgtttttg ttctcccact ggggtctgaa ttacctgcag
     3001 atcgcatccc ccgccgatgt cgtgatgggc ggtatcgaag tctttcttcg cttgattccg
     3061 tttttggctc tcgctgccgg caccagctgg cttggtcgaa tgttgcccat gaggggaatt
     3121 ggacaagcgc tgctgggagc gttctgcctc gtggcgatcc tcggctcttt tctgcttgtg
     3181 gcgcgggact tgccgaatga gggttcttca ttcggctggt ttcggccgtg gcgcgactac
     3241 gttgtctttg tcgccgttgc cgcgttcggg ggcttggcgg ccccgttggc agaacgcgac
     3301 ggagtagccg tgtcgttcaa cttgcttcta ggtttcacct gcttcttcgt tgctcttgta
     3361 ggcgttgaga ttggcgtttc acggggctac tggcccaacg aagccgtccc tcggtgggcg
     3421 gcgggcgtga ccgtcgagcc ttgtgatgaa ccacagttgc tttggagcgg tagcaacgcc
     3481 gttgtcttgg agtgcccagg ctatcgcgtt gtggtccgtg atgcgggggc actatccatc
     3541 agggtaccct accgtccgag gtgggaacga tcttatccga ggccgtgatc gcaatgcgcc
     3601 cacaagagtg tttgtgtttt gttcttgtgg agtcgtccgg cgcgggcgta ggagtctagc
     3661 cccggtcgga tggctccacg ggagacgaac atgcaggaca ataaccgacg cttcgccgac
     3721 gacatggcgc agctcgacgc tgagctaggc cgcgctatcg agcagagggc ttccctatcg
     3781 gcagcagagc aggaccgctt ccttggcgaa gacagttccc tcctccgtgg actgctggcc
     3841 ggcatgacct tcgcgggcgc tcccggccgc cgctgatcca atgagtgccg agttcccttg
     3901 gacgccttcc aacctgccgg agggct
//
LOCUS       DASVVN010000305         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_222538,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000305 DASVVN010000000
VERSION     DASVVN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222538"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VF686_04875"
     CDS             <1..1280
                     /locus_tag="VF686_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661179.1"
                     /translation="AVMDGIAARSAPGARVATFTVAGSVRGALAERGFSVAKRPGHGR
                     KRERLEAWLPEAAEEAAAPRVSIIGAGIAGAALARAFADLGLRATVVEAERPGAGASG
                     FPAALVTPRLDAGDAGIAALFAQALERARALYASVPGAILAEGVLQLEQQPRDASRFG
                     KIAGQPIWPDGAIAAVDAGTATGRVGEVVGGGLFMAEALALRPAPVLERWLEAADRIS
                     AGVAGVEAHDDRWRLVDGAGGTIIEADVVVIAAGWGAAGLWLEGPLSPVRGQADWVGG
                     VEASPVAWGGYAVPTGEGLLFGATHDRGETAIDVRPTDSARNLAVLAARLPELAARVE
                     AAGRGQARAAVRATTPDRLPLAGAVPGRPGLFVLGGLGSRGFCAAPLLAEHVAGLAVR
                     GASPLPRDLAARVDPARSSKTPGLAQPIGKADG"
     gene            1315..1737
                     /locus_tag="VF686_04880"
     CDS             1315..1737
                     /locus_tag="VF686_04880"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEX8661180.1"
                     /translation="MRALAASAVVSALALAACAPTDAGGSGPGMAANDPSSGRQCFSV
                     SQVNNYTKGGPGQVFLRVGRSAVYELQAAGGCQDLDFAQRVALVPDGGMAGSRLCTDD
                     WARVIVPGSTSAATVCRVRISRQLTEAQVAALPPRHRP"
     gene            complement(1734..>2437)
                     /locus_tag="VF686_04885"
     CDS             complement(1734..>2437)
                     /locus_tag="VF686_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8661181.1"
                     /translation="RLHTIRATRDEAACKAALEALTASATSGQGNLLDLAVQATRVRA
                     TVGEISDALEAAFGRHVASVKTVSGVYAKEAGPDPKISRARDMVAAFVENDGGPPRIL
                     IAKLGQDGHDRGQKVIATAYGDLGLEATAGSLFQTPSEAAKEAVERNVHAVGASSLAA
                     GHLTLVPELKAELEKLGRPDIMIVVGGVIPPSDVQTLIDMGAAAVYPPGSVIADTAID
                     LIEKLNQRLGYAQKP"
BASE COUNT          282 a          839 c          965 g          351 t
ORIGIN      
        1 acgcggtgat ggacgggatc gcggcgcggt cggcgccggg ggcgcgggtc gcgaccttca
       61 ccgtggcggg atcggtgcgg ggggcgcttg ccgagcgcgg cttttccgtg gccaaacggc
      121 ccggccacgg gcgcaagcgc gagcggctgg aggcctggct gcccgaggcg gcggaggagg
      181 ctgcggcacc gcgcgtgtcc atcatcggag ccgggatcgc cggcgcggcg ctggcgcggg
      241 ccttcgccga tctggggctc agggcgacgg tggtcgaggc ggaacgtccc ggcgcggggg
      301 cttcggggtt tccggccgcg ctggtcacgc cccggctgga cgcgggagac gccggcatcg
      361 ccgccctgtt cgcccaggcg ctggagcggg cgcgggcgct gtatgcgtcc gttccgggcg
      421 cgatcctggc cgaaggggtc ctgcaactgg agcagcagcc gcgcgacgcc tcacggttcg
      481 gaaagatcgc cggccagccg atctggccgg acggggcgat agcggcggtc gacgccggga
      541 ccgcgacggg gcgggtgggc gaggtcgtcg gcggcggcct gttcatggcc gaggcgctcg
      601 cgctgcggcc cgcgccggtg ctggagcgat ggctggaagc cgccgaccgg atcagcgccg
      661 gtgtcgccgg cgtcgaagcc catgacgacc gctggcggct ggtcgacggc gccggaggga
      721 cgatcatcga ggccgatgtc gtcgtcatcg ccgcgggctg gggcgcggcc ggtctgtggc
      781 tcgaggggcc gctgtcgccg gtgcgggggc aggccgactg ggtggggggc gtggaggctt
      841 ctccggtcgc ctggggcggc tatgcggtcc caaccggcga gggtctgctg ttcggggcga
      901 cccacgaccg gggcgagacg gcgatagatg tccggcccac cgacagcgcc cgcaatctgg
      961 cggtgctggc cgcccgcctg ccggagctgg ccgcgcgggt cgaggcggcg gggcgggggc
     1021 aggcgcgcgc cgccgtccgc gccacgacgc ccgatcgcct gccgctggcg ggcgccgttc
     1081 cgggaaggcc gggtctgttc gtgctggggg ggctgggctc gcgaggcttc tgcgccgcgc
     1141 ccctgctggc cgagcatgtc gccggcctcg ccgtccgggg cgcgtcgccg ctgccgcgcg
     1201 atctggcggc gcgggtcgat ccggcgcgat catcgaagac gccgggtctt gcgcaaccga
     1261 tcggcaaggc ggacggttga accgtcgtcg aagccccatc atccggagtt cgccatgcgc
     1321 gcccttgccg ccagcgccgt cgtttccgcc ctcgccctcg cagcctgcgc ccccacggat
     1381 gcgggcggtt caggcccggg catggccgcc aacgatccgt cgagcggccg ccagtgtttc
     1441 agcgtcagcc aggtcaacaa ctacaccaag ggcggcccgg ggcaggtctt ccttcgggtc
     1501 ggccgcagcg ccgtctacga actgcaggcg gccggcggct gtcaggatct ggatttcgcc
     1561 caacgggtgg cgctggtccc cgacgggggt atggccggtt cgcggctgtg caccgacgac
     1621 tgggcgcgcg tgatcgttcc gggctcgacc tcggcggcga ccgtctgccg cgttcgcatc
     1681 agccgccagc tgaccgaggc gcaggtcgcg gccctgccgc cgcgccacag accctaaggc
     1741 ttctgggcgt agcccagccg ctggttcagt ttttcgatca ggtcgatggc agtgtcggcg
     1801 atgaccgagc cgggcggata gacggcggcg gcgcccatgt cgatcagggt ctggacatcc
     1861 gacggcggga tgacgccgcc gaccacgatc atgatgtcgg gccgccccag cttttccagc
     1921 tcggccttga gctcggggac cagcgtcagg tggccagccg ccagagagga ggcgccgacg
     1981 gcgtggacgt tgcgctcgac cgcctccttc gccgcctcgg acggggtctg gaacagcgaa
     2041 ccggccgtgg cctccaggcc cagatcgcca taggcggtgg cgatgacctt ctggccgcgg
     2101 tcgtggccgt cctgaccgag tttggcgatc aggatgcgcg gcgggccgcc gtcgttctcg
     2161 acgaaggcgg cgaccatgtc gcgggcgcgg ctgatcttcg gatcgggccc ggcctccttc
     2221 gcatagacgc cggagaccgt cttgacgctg gccacgtgac gcccgaaggc ggcttccagc
     2281 gcgtcggaga tttcgccgac ggtcgcccgc acccgcgtcg cctgcaccgc gagatccagc
     2341 aggttgccct gcccgctcgt cgccgaggcg gtcagtgctt caagagcggc cttgcacgcc
     2401 gcctcgtcgc gcgtggcgcg gatggtgtgc agacgcg
//
LOCUS       DASVVN010000306         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_11258, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000306 DASVVN010000000
VERSION     DASVVN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11258"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF686_04890"
     CDS             complement(<1..212)
                     /locus_tag="VF686_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8661182.1"
                     /translation="MDGTDALLILVFAGLGLIVGSFTGLVSVRLPLGEGIVRGRSRCR
                     GCGRVLAPWNLVPVVSYVAARGRCAG"
     gene            503..2020
                     /locus_tag="VF686_04895"
     CDS             503..2020
                     /locus_tag="VF686_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661183.1"
                     /translation="MTGFRLTTLLAVSASALTLAACGSSEVASPGEGDFGNGGGGGGG
                     GGVTPPAPGTPAADCPAGLANVGVVANGTLRACQLPATITGALTLSVRAGTIYAISGR
                     TEVGTDRGADPAAPVAGSVQGVLTIEPGVKIFASGGTDYLLVNRGSQIFAEGTATNPI
                     IFTSRQNVEGTTTATSQGQWGGIVIAGRAPTAVCPVGVTAPNAACVGQVEGTSANYGG
                     NSPADNSGRLRYVQVNYSGFELSPNNELQALTLAGVGSGTTVDHFQSYNSSDDGIEIF
                     GGTVNLSHIIINGADDDGLDTDTGWRGGAQFVIVTQKPTAVTNGSRGFEWSAAPSGTA
                     FFAQPKVSNVTIVGRNAAVAADALITLNTGTDSSIINSVFTNPSTSAAACLDIDDANT
                     VANGQVFNSVYFACNRPFENDANIDAAATGAIFALGANNTSAGTSTMTAPAGATISNQ
                     GLAFINGANETAVTPANAPALHSFFVPVTYIGAVRNAADAWYAGWTCGLQAGSNC"
BASE COUNT          352 a          786 c          626 g          285 t
ORIGIN      
        1 cagccggcgc atcggccccg ggccgcgaca tagctcacga ccgggacgag gttccagggc
       61 gccagcaccc gtccgcaccc ccggcagcgc gatcgccccc gcacgatccc ttcgcccagc
      121 ggaagccgca cgctgaccaa acccgtgaag ctgccgacga tcagacccag accggcgaac
      181 acgaggatca ggagagcgtc cgtgccatcc atcaagacct gtccctccct gtttccaacg
      241 tccgttattg gccgccgcac gtccgtacaa gcgcgccata ccggcgctct gtgacagttc
      301 agccgtaccg ggatgggttt tcaccgaacc gcgaagacaa cttcacacaa gcgcaccgga
      361 accgtcacgg tacggcgtcg gccccgtcat atctgacccc taaccacagc ggtgggcagg
      421 gggcggtgca gtcatcgcgt cggggagcaa atccctgacg acccctgtcg tgaaattctt
      481 ttgcacagac acggtgaaaa cgatgaccgg cttccgactg acgaccctgc tcgcggtttc
      541 cgccagcgcc ctgaccctgg ccgcctgcgg ctcgtccgaa gtcgcgtcgc cgggcgaagg
      601 cgatttcggc aacggcggcg gtggcggcgg cggcggcggc gtgacgccgc cggcccccgg
      661 cacgccggcc gctgactgcc ccgccggtct ggccaacgtc ggcgtcgtcg ccaacggcac
      721 gctgcgggcc tgccaactgc ccgcgaccat caccggcgcc ctgaccctgt cggtccgcgc
      781 cggcaccatc tacgcgatca gcggccgcac cgaagtcggc accgaccgcg gcgccgatcc
      841 ggccgccccc gtcgccggca gcgtccaagg cgttctgacc atcgagccgg gcgtgaagat
      901 cttcgcctcg ggcggcaccg actatcttct ggtcaaccgc ggctcgcaga ttttcgctga
      961 aggcacggcg accaatccga tcatcttcac cagccgccag aacgtcgaag gcacgaccac
     1021 cgcgacctcg cagggccagt ggggcggcat cgtcatcgcc ggccgcgccc cgacggcggt
     1081 ctgcccggtc ggcgtcacgg cgccgaacgc ggcctgcgtc ggtcaggtcg aaggcaccag
     1141 cgccaactac ggcggcaact cgccggccga taactccggc cgtctgcgct acgtgcaggt
     1201 caactattcg ggcttcgaac tgtcgccgaa caacgagctg caggccctga ccctggccgg
     1261 cgtcggctcg ggcaccaccg tcgatcactt ccagtcgtac aacagctcgg acgacggcat
     1321 cgaaatcttc ggcggcacgg tgaacctgtc gcacatcatc atcaacggcg cggacgacga
     1381 cgggctggac accgacacgg gctggcgcgg cggcgcccag ttcgtgatcg tgacccagaa
     1441 gccgacggcc gtcaccaacg gcagccgcgg gttcgaatgg tcggccgccc cctcgggcac
     1501 cgccttcttc gcccagccca aggtctccaa cgtgaccatc gtcggccgca acgccgcagt
     1561 ggccgccgac gccctcatca ccctgaacac cggcacggac tcgtcgatca tcaactcggt
     1621 cttcaccaac ccgtcgacct cggccgcggc ctgcctggac atcgacgacg ccaacaccgt
     1681 cgccaacggc caggtgttca actcggtcta cttcgcctgc aaccgtccgt tcgagaacga
     1741 cgccaacatc gacgccgcgg ccacgggcgc catcttcgcc ctgggcgcca acaacacctc
     1801 ggcgggaacc tcgacgatga cggctccggc cggcgcgacg atctccaacc agggtctggc
     1861 cttcatcaac ggcgccaacg agacggcggt gaccccggcc aacgccccgg ccctgcacag
     1921 cttcttcgtg ccggtgacct acatcggcgc cgtgcgcaac gcggcggacg cctggtacgc
     1981 gggctggacc tgcggcctgc aggccggctc gaactgctga cgacggaaga cgggcggcgg
     2041 ctccttttc
//
LOCUS       DASVVN010000307         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_381734,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000307 DASVVN010000000
VERSION     DASVVN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381734"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..706)
                     /gene="ribD"
                     /locus_tag="VF686_04900"
     CDS             complement(68..706)
                     /gene="ribD"
                     /locus_tag="VF686_04900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX8661184.1"
                     /translation="MTTAKQASDDIRFMGRAIELAKAGMGQTWPNPSVGCVIVRDGEV
                     VSEAATAPGGRPHAEEQAVPAAGDRARGATAYVTMEPCGARSSGRTSCSNFLMDAGVA
                     RVVVAAVDPSPFAAGRGVERLKKAGLEVETGLLAQEAAVLYEGYLHRVETGRPMVRIS
                     EDGAGYDARFAASAKADLTNELQRLGEAGYTRVWVSPGELADALEAQGLLTA"
     gene            complement(703..1419)
                     /gene="gpmA"
                     /locus_tag="VF686_04905"
     CDS             complement(703..1419)
                     /gene="gpmA"
                     /locus_tag="VF686_04905"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX8661185.1"
                     /translation="MPRLILLRHGQSQWNLENRFTGWVDVDLTAEGEAQARRGGELIR
                     DAGFHPAVMFTSVLTRAKRTGALALDSAGLKDVPVIEDWRLNERHYGGLTGLDKAETA
                     ALHGDAQVKIWRRSYDIPPPPLAPGGEWDFSADPRYAGQTIPDTESLKTTLDRVLPYW
                     DAAIAPRLRAGDDVLIAAHGNSLRAIVKHLFAVPDDQIVGVEIPTGNPLEIDLDANLK
                     PVAARYLDAGRATELPKVSE"
     gene            1579..>3007
                     /locus_tag="VF686_04910"
     CDS             1579..>3007
                     /locus_tag="VF686_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8661186.1"
                     /translation="MIKPRQDLRPNTADYETVPLVKPTGFREYDARWILEKEINLLGI
                     QALGLGLATYVHEIGQTPRIVVGHDYRSYSLSVKQALTLGLLEGGMEVLDIGLALSPM
                     AYFAQFELEAPCVAMVTASHNENGWTGVKMGAQPPLTFGPDEIGRLKDIVLNGEGVGR
                     KGGSLTRVEGFREAYLADIVSKVKLTRPIKVLAACGNGTAGAFAPEALRRMGAEVIEM
                     DTELDYTFPKYNPNPEDHAMLMQMAQRVRDTGADLALGFDGDGDRCGVVDDTGEEIFA
                     DKIGLMLGRDLSALHPDATFVVDVKSTGLYKTDEVLKANGATTVYWKTGHSYIKRKTA
                     ELGALAGFEKSGHFFFNAPLGHGYDDGIVAAGAVLAMLDRNPGKKLSELKAALPDAWT
                     SLTMSPHCDDELKYEVLERIVAEYQALADAGGSILGRKIVEAITVNGVRVHLEDGSWV
                     LVRASSNKPELVVVVESMRSEADMKA"
BASE COUNT          470 a          974 c         1089 g          474 t
ORIGIN      
        1 ggaaggctga aatggttacc gcgagaacct tgtttccgcc ttacgcgaca tgctgaaccc
       61 gtcggggtca ggcggtaagc aaaccctgcg cttccagcgc gtcggccagt tctccggggc
      121 tgacccagac ccgggtgtag ccggcctcgc ccagacgctg cagttcgttg gtcaggtcgg
      181 ccttggccga ggcggcgaag cgggcgtcgt agccggcgcc gtcctcgctg atgcggacca
      241 tcggccggcc ggtctcgacg cggtgcagat agccttcgta gagaaccgcc gcttcctggg
      301 ccaacaggcc ggtctcgacc tcgagccccg ccttcttcag ccgctcgacg ccccggcccg
      361 cggcgaaggg cgagggatcg acggcggcga cgacgacgcg ggcgacgcca gcgtccatca
      421 ggaagttgga gcaggaggtg cggcccgatg agcgcgcgcc gcagggctcc atggtcacat
      481 aggcggtggc gccacgcgcc ctgtcgccgg cggcgggaac ggcctgttcc tcggcgtggg
      541 gccggccgcc aggggcggtc gcggcttcgg agacgacctc gccgtccctg acgatgacgc
      601 agccgaccga ggggttgggc caggtctgcc ccatgccggc cttggccagt tcgatggccc
      661 ggcccatgaa gcggatgtcg tccgaggcct gtttggcggt ggtcattcgc tcaccttcgg
      721 cagttcggtc gcccggccgg cgtccagata gcgggcggcc acgggcttga ggttcgcatc
      781 cagatcgatc tcgagcgggt tgccggtcgg aatctcgacg ccgacgatct ggtcgtccgg
      841 cacggcgaac aggtgtttga cgatggcgcg cagggagttg ccgtgggcgg cgatcagcac
      901 gtcgtctccg gcccgcagtc gcggcgcgat ggcggcgtcc cagtacggca ggacccggtc
      961 cagcgtggtc ttcaggcttt cggtgtcggg aatggtctga ccggcgtagc gcgggtcggc
     1021 gctgaagtcc cattcgccgc ccggggccag cggcggcggc ggaatgtcgt agctgcggcg
     1081 ccagatcttc acctgggcgt cgccgtgcag ggcggcggtc tcggccttgt ccagaccggt
     1141 caggccgccg tagtggcgct cgttcagccg ccagtcctcg atgaccggaa cgtccttcag
     1201 gccggcggag tccagcgcca gggcgccggt gcgtttggcc cgcgtcagca ccgaggtgaa
     1261 catgacggcg ggatggaagc ccgcgtcgcg gatcagttcg ccgccgcgcc tcgcctgggc
     1321 ctcgccctcg gcggtcaggt caacgtcgac ccagccggtg aatcggttct cgaggttcca
     1381 ctggctctgg ccatggcgca gcaggatcag gcggggcatt caggtctccg gtcgtcgagg
     1441 ctttgggtag gcccagggtc gggaccggtc aaggttcggc tccctgacgc caacgtggcg
     1501 ccgaaaccgg cgttcccgcg ttgttgtgac cccggaagcg cctgctatac cgggcgccgc
     1561 ttctacagga ccgcgaccat gatcaaaccc cgccaggacc ttcgccccaa cacggccgac
     1621 tatgagaccg tgccgctggt gaagccgacg ggctttcgcg aatacgacgc gcgctggatc
     1681 ctggagaagg agatcaacct gctgggcatc caggcgctcg gcctcgggct ggcgacctat
     1741 gtgcacgaga tcggtcagac tccgcggatc gtggtcggtc acgactaccg gtcctactcc
     1801 ctgtcagtga aacaggcgct gacactgggg ttgctggaag gcgggatgga ggtgctggac
     1861 atcggtctgg ccctgtcgcc gatggcctac ttcgcccaat tcgagctgga ggcgccgtgc
     1921 gtggccatgg tcacggccag ccacaacgag aacggctgga ccggggtcaa gatgggggcg
     1981 cagccgccgc tgaccttcgg ccccgacgag atcgggcggc tgaaggacat cgtcctgaac
     2041 ggcgaaggcg tgggccgcaa gggcggctcg ctgacccgcg tcgagggctt ccgcgaggcc
     2101 tatctggccg acatcgtgtc caaggtgaag ctgacccggc cgatcaaggt cctggcggcc
     2161 tgcggcaacg gcacggccgg ggccttcgcc cccgaggccc tgcggcggat gggggccgag
     2221 gtgatcgaga tggataccga gctcgactac accttcccca aatacaatcc gaacccggaa
     2281 gaccacgcca tgctgatgca gatggcgcag cgggtgcgcg acacgggcgc cgatctggcg
     2341 ctgggtttcg acggcgatgg cgaccgctgc ggtgtggtgg acgatacggg cgaggagatc
     2401 ttcgccgaca agatcggcct gatgctgggc cgcgacctgt cggccctgca ccccgacgcc
     2461 accttcgtcg tcgatgtgaa atcgaccggc ctgtacaaga ccgacgaggt gctgaaggcc
     2521 aatggcgcca ccaccgtcta ctggaagacg ggccacagct acatcaagcg caagaccgcc
     2581 gaactggggg cgctggccgg gttcgagaag tcgggccact tcttcttcaa cgccccgctg
     2641 ggtcacggct acgacgacgg catcgtcgcg gcgggggcgg tgctggccat gctggaccgg
     2701 aaccccggca agaagctgag cgagctgaag gccgccctgc cggacgcctg gacgtcgctg
     2761 acgatgtcgc cgcactgcga cgacgagctg aaatacgagg tgctggagcg gatcgtggcc
     2821 gagtatcagg ccctggcgga cgccggcgga tcgatcctgg ggcggaagat cgtcgaggcg
     2881 atcaccgtca acggcgtgcg ggtgcatctc gaggacgggt cctgggtgct tgtccgcgcc
     2941 tcgtccaaca agccggagct ggtggtggtg gtcgagagca tgcgctccga ggccgacatg
     3001 aaggcgc
//
LOCUS       DASVVN010000308         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_64109, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000308 DASVVN010000000
VERSION     DASVVN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64109"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /gene="mltG"
                     /locus_tag="VF686_04915"
     CDS             <1..909
                     /gene="mltG"
                     /locus_tag="VF686_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8661187.1"
                     /translation="DRKLRAGEYEVPSKASLRSVIVLLVEGRVVRHYVTLPEGWSSAQ
                     AVDILNRQPILIGTVDVTPDEGALWPDTYEVSRGETRNSVVARMRRARDATLARLWAA
                     RSPSTVVRSPEEAVILASIVEKETGIAFERPRVAAIFTNRLRAGMRLESDPTIVYGIT
                     RGRPLGRGIRRSELDRATAWNTYQIDGLPPTPIANPGRDAIAAVLNPPRTDELFFVAD
                     GTGGHVFARTYDEHLANVARWREIERRKAGLPPEAAPPAVPGTVPGVPNAPTDAASGA
                     AAPPGVTVPPGARVISIPPAAPAPTR"
     gene            906..1790
                     /locus_tag="VF686_04920"
     CDS             906..1790
                     /locus_tag="VF686_04920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX8661188.1"
                     /translation="MSQISGMTGFGRADGSHGDWTWSVEARSVNGRNLEVRFRGPPGF
                     DTLERHAKASAQTRLNRGQVTLGVQAKRADAGAASLKVNQDILARYLALANELAEEGA
                     TPPSADGLLSLRGVLEAPEEADDADARAAVEAAMAITIDQALDALKLSRDREGGQLLP
                     VVTDFIDRIDTLVAQAETEATAQTDAIRDRFTRRMTELAPDAPGLQDRIFLEAAALAT
                     KADVREELDRLTAHVASARTLLQQPPAGRKLDFLMQEFMREANTLCSKSATTALTGIG
                     LELKAVIEQLREQVQNVE"
     gene            1780..2421
                     /gene="gmk"
                     /locus_tag="VF686_04925"
     CDS             1780..2421
                     /gene="gmk"
                     /locus_tag="VF686_04925"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8661189.1"
                     /translation="MSNERTPRRGVLLIVASPSGAGKTSLCRRLMADHGGLELSISMT
                     TRGIRPGEVADRDYHFVDDAEFQRLIDADAFLEWADVHGARYGSPRAPIDLALAEGRD
                     VLFDIDWQGARDIAVKCPEDAVRVFILPPSLEELRRRLVTRSQDADDVIERRIANAKG
                     EIEHCGDFDYIFVNDDFDRSYAELAHIYHAERSKRFRNVWVDAYRNALLNEAV"
     gene            complement(2635..>2737)
                     /locus_tag="VF686_04930"
     CDS             complement(2635..>2737)
                     /locus_tag="VF686_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEX8661190.1"
                     /translation="GGGALCEAAGIEPDARAEVIDGAGFLRLAQALG"
BASE COUNT          429 a         1090 c          841 g          377 t
ORIGIN      
        1 gaccgcaagc tgcgcgccgg cgagtacgag gtgcccagca aggcgtccct gcggtccgtc
       61 atcgtcctgc tggtcgaggg ccgggtggtc cgccactacg tcaccctgcc ggagggctgg
      121 tcctcggcgc aggcagtcga catcctcaac cgccagccga tcctgatcgg cacggtcgac
      181 gtcacccccg acgaaggcgc cctctggccc gacacctatg aggtcagtcg cggcgagacg
      241 cgcaactcgg tcgtcgcccg catgcgccgc gcccgcgacg cgaccctggc ccgcctctgg
      301 gccgcccgca gcccgtccac ggtcgtccgg tcgcccgagg aagccgtcat cctcgcctcc
      361 atcgtcgaga aggaaaccgg catcgcgttc gaacgcccgc gcgtcgccgc catcttcacc
      421 aaccgtctgc gcgccggcat gcgtctggag agcgatccga ccatcgtcta cggcatcacc
      481 cgcggccgcc cgctgggccg gggcatccgg cgctccgagc tggaccgggc cacggcctgg
      541 aacacctacc agatcgacgg cctgcccccg acgccgatcg ccaatcccgg ccgcgacgcc
      601 atcgccgccg ttctcaaccc gccccgcacc gacgagctgt tcttcgtcgc cgacggcacc
      661 ggcggccacg tcttcgcccg cacctacgac gagcatctgg cgaatgtcgc ccgctggcgc
      721 gagatcgagc gccgcaaggc cggcctgccg cccgaggctg ccccgcccgc cgtgcccggc
      781 acggtcccgg gcgtgcccaa tgcgccgacg gacgccgcat caggcgccgc cgcgcctccc
      841 ggcgtgaccg tcccgcccgg cgcgcgcgtc atcagcattc cgccagccgc ccccgcgccg
      901 acccgatgag ccagatctcc ggcatgaccg gcttcggccg cgcggacggt tcccacggcg
      961 actggacgtg gtcggtcgag gcgcgctcgg tcaacggccg caatctggag gtccgcttcc
     1021 gcggcccgcc cggtttcgac accctcgagc gtcacgccaa ggcttcggcc cagacgcgcc
     1081 tcaaccgcgg gcaggtcacg ctcggcgtcc aggccaaacg cgccgacgcg ggcgccgcct
     1141 ccctgaaggt caatcaggac atcctcgccc gctacctcgc cctcgccaac gagctggcgg
     1201 aggagggggc gaccccgccc tccgccgacg gcctgctgtc cctgcgcggc gtgctggaag
     1261 cgcccgagga ggccgacgac gccgacgccc gcgccgccgt cgaagccgcc atggccatca
     1321 ccatcgatca ggccctcgac gccctgaagc tgtcgcgcga ccgcgagggc ggccagcttc
     1381 tacccgtcgt caccgacttc atcgaccgca tcgacacgct cgtcgcccag gccgaaaccg
     1441 aagcgaccgc ccagaccgac gccatccgcg accgcttcac ccgccgcatg accgaactgg
     1501 cgcccgatgc gcccggcctt caggaccgca tcttcctcga ggcggcggcg ctggcgacca
     1561 aggccgacgt gcgcgaggaa ctggaccggc tgaccgccca cgtcgcctcg gcccgcaccc
     1621 tgttgcagca accccccgcc ggccggaaac tcgacttcct gatgcaggaa ttcatgaggg
     1681 aggcgaacac cctctgctcg aaatcggcta cgaccgcgct caccggaatc ggccttgagc
     1741 tgaaggccgt catcgaacag ttgcgcgaac aggtccagaa tgtcgaatga acgcacgccc
     1801 cgccgcggcg tcctcctgat cgtcgccagc ccgtcgggcg cgggcaagac cagcctgtgc
     1861 cgccgcctga tggccgacca cggcggactg gagttgtcga tctccatgac cacccgcggc
     1921 atccgccccg gcgaggtcgc cgaccgcgac taccacttcg tcgacgacgc ggaattccag
     1981 cgcctgatcg acgccgacgc cttccttgaa tgggccgacg tccacggcgc ccgctacggc
     2041 tcgccgcgcg ccccgatcga tctggccctg gccgagggcc gcgacgtcct gttcgacatc
     2101 gactggcagg gcgcccgcga catcgccgtg aaatgcccgg aggacgccgt ccgcgtcttc
     2161 atcctgccgc ccagcctcga ggagctgcgc cgccgtctgg tgacccgctc gcaggacgcc
     2221 gacgacgtca tcgaacgccg catcgccaac gccaaaggcg agatcgagca ctgcggggac
     2281 ttcgactaca tcttcgtcaa cgacgacttc gaccgcagct acgccgaact ggcccacatc
     2341 taccacgccg aacggtcgaa gcggttccgc aatgtctggg tcgacgccta ccggaacgcg
     2401 ctgctgaacg aggcggtcta ggccggtctt gaagacagtt ccgaagggcc tacatccaac
     2461 ctgttgttcc aacattgaaa ggaggtggtc gaatgtctca ttgtctcacg ccgctcgagg
     2521 cccatcggat gagcctgacc tcccctctgg cggggttctg cgcctgatct gaagccgtga
     2581 tcggctgaca atgtaagggc cgctccatcg gggcggccct ttgcaattct gccgtcagcc
     2641 aagcgcctgc gccaatcgca ggaagccggc cccgtcgatc acctccgctc gcgcgtccgg
     2701 ctcgatcccc gccgcttcgc agagcgcccc gccgcca
//
LOCUS       DASVVN010000309         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_64241, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000309 DASVVN010000000
VERSION     DASVVN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64241"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..777
                     /locus_tag="VF686_04935"
     CDS             109..777
                     /locus_tag="VF686_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX8661191.1"
                     /translation="MSESILKDYLATAGQEPSSARIIDLPTPDEADAQRELVRIPARA
                     MGVATAADLRDYFRLPLADARDRIAELVEAGELTPVAVKGWRQQAYLAAGARSPRKIA
                     GAALLSPFDNLIWARDRTERLFDVRVRLEIYTPAHKRAHGYYVLPFLEDEAITARVDL
                     KSDRKAGTLLVQAAWREPDATPETAARLAAELKRMAGWLGLEGVEVVGKGDLAGKLKS
                     AISS"
     gene            complement(986..2494)
                     /locus_tag="VF686_04940"
     CDS             complement(986..2494)
                     /locus_tag="VF686_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8661192.1"
                     /translation="MTAANEAKTILQRLGVDDAVWGGALPIRSPVDGSSAGPLVETGD
                     LDGVAARSVAAFHAWKRVPAPRRGELVRLLGEELRASKDDLARLVTLEAGKIVSEGLG
                     EVQEMIDICDFAVGLSRQLYGLTLASERPGHHMRETWQPLGPVLVISAFNFPVAVWAW
                     NACLALVCGDPVIWKPSEKTPLTALATQAIFDRAIARFAKETGNGAPAGLSQLVIGGR
                     DAGETLTADPRIALVSATGSTRMGRAVAQTVAVRLGRSLLELGGNNAMIVTPSADLDM
                     AVRAIAFSAVGTCGQRCTTLRRLLVHESIADGLIARLKAAYETLPIGDPRQDGILVGP
                     LIDDASAHGFETALAQAVEQGGQIVVGGDRVDREGVYVRPAIVRMPAQTAVVQHETFA
                     PILYVLTWSDFDDAVAMQNAVPQGLSSCVFTDSVREAEQFLSAWGSDCGIANVNIGPS
                     GAEIGGAFGGEKDTGGGRESGSDAWKAYMRRQTQTVNFSTSLPLAQGVKFEV"
     gene            complement(2592..2981)
                     /locus_tag="VF686_04945"
     CDS             complement(2592..2981)
                     /locus_tag="VF686_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661193.1"
                     /translation="MKTAMIRKALLSTLAATAVMTATPALAQNVTFSIGVGSPAYGQG
                     YGYNRGYGADLQYRAQRVAQQINRVAYNGRISRGEAQRLSWEMQQYRNLEWRYARDGL
                     SRAEYAALSNRLTRIEYMLRQERRDRW"
     gene            3165..>3311
                     /locus_tag="VF686_04950"
     CDS             3165..>3311
                     /locus_tag="VF686_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8661194.1"
                     /translation="MSKDVQVARPGDTVQEVASRMAAGDFGFMPVADGDTLIGTITDR
                     DLTIR"
BASE COUNT          508 a         1077 c         1217 g          509 t
ORIGIN      
        1 ccggcgctgg cacagactta cagcggcctg tggcacaggg ctccggcagg atacgctgca
       61 gaaaacggtc atcctgtgct gcgccccgag cggttttcag cattcaccgt gtctgagtcg
      121 atcctgaagg attatctggc aacggcaggg caggagccct cgtcagcccg gatcatcgac
      181 ctgccgacgc cggacgaggc ggacgcccag cgcgagctgg tgcgcatccc ggcgcgggcc
      241 atgggggtgg cgacggcggc ggacctgagg gattatttcc ggctgccgct ggccgatgcg
      301 cgcgatcgga tcgcggaact ggtcgaggcc ggggagctga cgccggtggc ggtgaagggg
      361 tggcggcagc aggcgtatct ggccgccggg gcgcggtcgc cgcgcaagat cgccggcgcg
      421 gcgctgctgt cgccgttcga caatctgatc tgggcccgcg accggaccga gcggctgttc
      481 gacgtcaggg tgcggctgga gatctacacc cccgcgcaca agcgggcgca cggctattac
      541 gtcctgccgt ttctcgagga cgaggcgatc acggcgcgcg tcgacctgaa gtcggaccgc
      601 aaggccggga cgttgctggt gcaggcggcg tggcgcgagc cggacgcgac gccggaaacg
      661 gccgcgcggc tggcggcgga gctgaagcgg atggccgggt ggctggggct ggagggggtg
      721 gaggtggtcg gcaaggggga tctggccggg aaactgaaat cggcgatcag ttcgtgacga
      781 tttcggctac gcaatcgcgg tgatttcgat cagtcggctg tgaaggaaac tgacggtttg
      841 cgacgttctc cctccccttc atggggaggg acgatggcga agccatccgg gtggggtccg
      901 aaagagctcg agcgcggacc ggcgtgcccc acctgtcgtc gcttcgcgcc gacatccctc
      961 cccacaaggg ggagggagaa gcagatcaca cctcgaattt cacgccctgc gccagcggca
     1021 ggctggtgga gaaattcacc gtctgggtct ggcggcgcat ataggccttc cacgcgtccg
     1081 acccggattc gcgcccgccg ccggtgtcct tctctccccc gaaggctccg ccgatctcgg
     1141 cgcccgaggg gccgatgttg acgttggcga tgccacagtc cgagccccag gccgacagga
     1201 attgctcggc ttcgcggacg ctgtcggtga agacgcagga gctcaggccc tgcggcacgg
     1261 cgttctgcat ggccacggcg tcatcgaagt cggaccatgt caggacgtag aggatagggg
     1321 cgaaggtctc gtgctggacc accgctgtct gcgccggcat gcggacgatg gcggggcgga
     1381 cgtagacgcc ctcacggtcg acgcgatcgc cgccgacgac gatctggccg ccctgctcga
     1441 ccgcctgcgc cagggccgtt tcgaagccgt gcgccgaggc gtcgtcgatc agcggaccga
     1501 cgaggattcc gtcctgacgc gggtcgccga tcggcagggt ctcataggcc gccttgagcc
     1561 gcgcgatcag gccgtcggcg atgctttcgt gcaccagcag gcggcgcagg gtagtgcagc
     1621 gctggccaca ggtgccaacg gccgagaagg cgatggcgcg gacggccatg tcgaggtcgg
     1681 cgctgggcgt gacgatcatg gcgttgttgc cgccgagctc gagcagcgag cggccgaggc
     1741 ggacggcgac cgtctgcgcg acggccctgc ccatccgcgt cgagccggtg gcggagacga
     1801 gggcgatgcg cgggtcggcg gtcagggtct cgcccgcgtc gcggccgccg atgaccagct
     1861 gggacaggcc tgcgggcgcc ccatttcccg tttctttggc gaaccgggcg atggcgcggt
     1921 cgaagatcgc ctgcgtcgcc agcgcggtca gcggcgtctt ctccgacggc ttccagatca
     1981 ccggatcgcc gcagaccagc gccagacagg cgttccacgc ccagaccgcg accgggaagt
     2041 tgaaggccga gatgaccagc accggcccca gcggctgcca ggtctcgcgc atgtggtggc
     2101 cggggcgttc cgaggcgagc gtcaggccgt agagctggcg cgacaggccg acggcgaaat
     2161 cgcagatgtc gatcatctcc tgcacctcgc cgaggccctc ggagacgatc ttgccggctt
     2221 cgagggtgac gagacgggcg aggtcgtcct tcgaggcgcg cagctcctcg ccgagcaggc
     2281 ggaccagttc gccgcgccgg ggcgccggca cgcgcttcca ggcgtggaag gcggcgacgg
     2341 agcgggcggc gacgccatcc agatcgccgg tctcgaccag cggacccgcg ctggacccgt
     2401 cgacgggcga ccggatcggc agggcgccgc cccagacggc gtcgtccacg cccagccgtt
     2461 gcaggatggt cttcgcctcg ttcgccgccg tcatcttcgc tctcccgttc gagcgtgcgg
     2521 cttagccgca gagccggaca aacgaaaggc cgccccgtcg ccggggcggc cctccattca
     2581 cgtcgccgga cttaccagcg gtcgcggcgc tcctgacgga gcatgtattc gatgcgggtc
     2641 agacggttgc tgagcgctgc atattccgca cggctcaggc cgtcgcgggc gtagcgccac
     2701 tcgaggttcc ggtactgctg catctcccag ctcaggcgct gggcctcgcc gcggctgata
     2761 cggccgttgt aggccacccg gttgatctgc tgggccacgc gctgggcgcg gtattgcagg
     2821 tcggcgccat agccgcggtt gtagccgtaa ccctggccgt aggcgggcga gccgacgccg
     2881 atggagaagg tgacgttctg ggccagcgcc ggcgtggccg tcatgaccgc cgtcgccgcc
     2941 agcgtggaaa gcagggcttt ccggatcatg gcggttttca ttgtcgtatt ccttgttccc
     3001 gatccgcagc ggaccgtgga cacaggaaac cacgccgagg ctgacacgac cctgagggcc
     3061 ctgttgtccg ccagtcatct cgtccgggcc ggaaccggcg aggcggggga gggttttgac
     3121 ggcagcccct ttcaaggaga gacccgatga aaatccgcga cgtgatgagc aaggacgtcc
     3181 aggtcgcccg gcccggcgac accgtgcagg aggtcgcctc gcgtatggcg gccggagatt
     3241 tcggcttcat gcccgtggcg gacggcgaca ccctgatcgg caccattacg gatcgggacc
     3301 tgacgatccg g
//
LOCUS       DASVVN010000310         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_325656,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000310 DASVVN010000000
VERSION     DASVVN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325656"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1113
                     /gene="prfA"
                     /locus_tag="VF686_04955"
     CDS             46..1113
                     /gene="prfA"
                     /locus_tag="VF686_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX8661195.1"
                     /translation="MRLPQARLDQVLDRFHQVEARMGAASDGAEIVRLSKEHAEMKPV
                     ADAVMALAKTRAEMADLEAMAADPEMAEMAAEELQTLKETLPDMERDVALLLAPRDAD
                     ENASAVLEVRAGAGGDEAALFAGDLFRMYSRYAANRGWKVELDSATEGDAGGYKEIIA
                     TVTGDGVFGRLKFESGVHRVQRVPSTEAQGRIHTSAATVAVLPEVEDVEIDIQDKDIR
                     IDTFRASGSGGQHVNKTDSAVRITHFPSGIVVTSSEKSQHVNRDKAMKNLRVRLYDMQ
                     RQMKDNARSDARKSQVGSGDRSERIRTYNFPQGRVTDHRIGLTLHSLPQILEGDIDPL
                     LNALIAEDQAARLADLEAEFG"
     gene            complement(1158..>2301)
                     /locus_tag="VF686_04960"
     CDS             complement(1158..>2301)
                     /locus_tag="VF686_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8661196.1"
                     /translation="GAGAAHLLHTRGGKLALTLSPEPAEPASTGVLTPEDFDTKEPGR
                     GRLAQRPEHIPHKGWVDIFWRLGMSYFGDRIGFLSGGVTFFVMLSLFPTLAAFVTVYG
                     LFADPTDAAARVSFLYSVMPSGVASFLSGEMQRLAENSNTQLTFTLVWTLSLSLWTAN
                     GAIKVLFYGLNVAYHEVEKRNIVKYNLMCMAFTVGGLASILITAAIIVGVPLVLGVFG
                     WAAEWSYLAPFRWPVLLLVYIAALTLIYRMGPCRQKARWRWLTPGAIFAALLSLTLSL
                     IFSWYLQTFVRTASYGPLAAMMGFLLWTWLSVQIILMGAELNAEIEHQTAMDTTTGHP
                     LAIGERGAVVADTVGPKRGSPAALAFTLKHAEALSDRLLRRKIRPK"
BASE COUNT          423 a          809 c          757 g          312 t
ORIGIN      
        1 ggcggggtgg gccggatagg agatcggccc ctcccggaga aacccttgcg actgccccag
       61 gcccggctcg atcaggtgct cgaccgcttc catcaggtgg aggcgcggat gggggctgcg
      121 tccgacgggg ccgagatcgt gcgcctgtcg aaagagcatg cggagatgaa gcccgtggcc
      181 gatgcggtca tggcgctggc gaagacgcgc gccgaaatgg ccgacctcga ggccatggcc
      241 gccgatcccg agatggccga aatggccgcc gaggagttgc agaccctcaa ggagaccctg
      301 ccggacatgg agcgggacgt cgccctgctg ctggccccgc gcgacgccga cgagaacgcc
      361 tcggccgtac tggaagtgcg cgccggcgcc ggcggcgacg aggccgccct gttcgccggc
      421 gacctgttcc gcatgtattc gcgctacgcc gccaaccgcg gctggaaggt ggagctggac
      481 tcggcgaccg agggcgacgc cggcggctac aaggagatca tcgccaccgt caccggcgac
      541 ggcgtcttcg gccggctgaa gttcgaaagc ggcgtccacc gggtgcaacg cgtgccctcg
      601 accgaggcgc aagggcgcat ccacacctcg gccgccaccg tcgccgtcct gcccgaggtc
      661 gaggatgtcg agatcgacat ccaggacaag gacatccgca tcgacacctt ccgcgcctcc
      721 ggctcgggcg gtcagcacgt caacaagacc gattccgccg tgcgcatcac ccacttcccc
      781 tcggggatcg tggtcacctc gtccgagaag tcgcagcatg tgaaccgcga caaggcgatg
      841 aagaacctgc gcgtccgcct ctacgacatg cagcgccaga tgaaggacaa cgcccgttcc
      901 gacgcccgca aatcgcaggt cggctcgggc gaccggtcag agcgcatccg cacctacaac
      961 ttcccgcagg ggcgggtgac cgaccaccgc atcggcctga ccctgcacag cctgccgcag
     1021 atcctcgagg gcgacatcga ccccctgctg aacgccctca tcgccgagga tcaggcggcg
     1081 cgtctggccg atctggaggc ggagttcggg taagcatcgc gttctccctc cccttcatgg
     1141 ggagggagaa gccctcatca cttcggccgg atcttccgcc gcagcagccg gtccgacagc
     1201 gcctcggcgt gtttcagggt gaacgccagc gccgccggcg aaccgcgttt cggcccgacc
     1261 gtatcggcca ccaccgcgcc ccgttcgccg atcgccagcg gatggcccgt cgtggtgtcc
     1321 atcgccgtct ggtgctcgat ctcggcgttc agctcggcgc ccatcaggat gatctgcacg
     1381 ctcagccagg tccacagcag gaagcccatc atcgcggcca gcggaccata ggacgccgtg
     1441 cgcacgaagg tctgcaaata ccagctgaag atcagcgaca gggtcaggct cagcagggcg
     1501 gcgaagatcg cgcccggcgt cagccagcgc cagcgcgcct tctggcggca cggccccatg
     1561 cggtagatca gcgtcagggc ggcgatatag accagcagca gcaccggcca ccggaacgga
     1621 gccagatagc tccactcggc cgcccagcca aacaccccca gcaccagcgg cacgcccacg
     1681 atgatggccg ccgtgatcag gatcgaggcc agcccgccga ccgtgaaggc catgcacatc
     1741 aggttgtact tgacgatgtt gcgcttctcg acctcgtgat aggcgacgtt cagcccgtag
     1801 aagagcacct tgatcgcccc gttggcggtc cacagcgaca gcgacagggt ccagaccagg
     1861 gtgaaggtca gctgggtgtt cgaattctcg gccagccgct gcatctcgcc ggacaggaag
     1921 ctggccaccc ccgagggcat caccgaatag aggaaggaca cgcgcgcggc ggcgtccgtc
     1981 ggatcggcga acaggccgta gaccgtcacg aaggccgcca gcgtcgggaa cagcgacagc
     2041 atgacgaaaa aggtcacgcc gccggacagg aagccgatcc ggtcgccgaa ataggacatt
     2101 cccagccgcc agaagatgtc gacccagccc ttgtgcggaa tatgctccgg ccgctgcgcc
     2161 agacgcccgc gccccggttc tttcgtatcg aagtcctccg gcgtcagcac cccggtggac
     2221 gccggctcgg ccggctcggg cgacagggtc aacgcgagct tgccgcctcg cgtgtgaagc
     2281 agatgcgccg cgccggcgcc g
//
LOCUS       DASVVN010000311         5426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_222735,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000311 DASVVN010000000
VERSION     DASVVN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222735"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF686_04965"
     CDS             <1..497
                     /locus_tag="VF686_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1453 domain-containing protein"
                     /protein_id="HEX8661197.1"
                     /translation="AVIVILLRNRQKRTLRPELMWILPAIFIPLIAFGLWGMRQTPGA
                     DLTPYGPADWGLLAGCAVLGALGGFWRGSMVTIEKDPGGVLKAQASPLGIILIIVLLA
                     VRSFARPWLEGHAESLGLHIVAVEQGFLLLAGGLIVAQRIEMFIRARRIMAGAPDPHV
                     EVVA"
     gene            494..1261
                     /locus_tag="VF686_04970"
     CDS             494..1261
                     /locus_tag="VF686_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661198.1"
                     /translation="MRAIIFAFLAAIVAGPVLAQGAEEDWDLREDPQQQLTLATLNFG
                     DNQIALRCRAGVLDLLLTGVPPTDAATRTVRVTAGAIRNESQVWFTRAGQPITSTGEP
                     ERLARQLRAGRELDVLLEATGPGERPRRYRLPVPASAVSVDRVLTACSVPLTDDWDLR
                     PRAEPGLIVWDSQPRPDFPSLAIERGFEQAEVRLACLVAAAGALDECRILSETPAGAG
                     FGQSAQRSAARARLGLPADDATVIGTVISFTIRFRAS"
     gene            1331..2158
                     /locus_tag="VF686_04975"
     CDS             1331..2158
                     /locus_tag="VF686_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661199.1"
                     /translation="MKRILSALLACAVALPALAQDASADWDLMTDQRRRLTVAYTRFD
                     NGVTVATRCADGGFETIVTGLPPAGAATRRTLRIAFGDDDLFDSQWSVATDDTVAVAD
                     LPAPFARRMRAGGRLRILVPNGATEGRNLLYDLQLPASSGAIDQTLTSCGRPLVDPRD
                     AELNALPDDGVPGTIAWAVRPRPQYPETRYARGFAVITCLTNPDGSLRDCAIESEHPH
                     DGRFGRPTLASARRARLQNIAEPGAPVPAAQVLYKVNYVMDGYQTREEERARRPARG"
     gene            complement(2155..3195)
                     /locus_tag="VF686_04980"
     CDS             complement(2155..3195)
                     /locus_tag="VF686_04980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8661200.1"
                     /translation="MRYGYWAPVFGGWLRNVDDERAATWENLSRLVRRSEEIGWDLTL
                     IAELNLNDIKGIEAPALDAWSSAGALAAVTKTIELMVAVRPNFHQPALFAKAAANIDQ
                     ISGGRLALNVVSSWWAKEAESYGLQFDQHDDRYARTTEWLQVVDRLWTQRRTDFEGRY
                     YSLKDAIVEPKPTSTPARPRPVIYAGGESEAAKTLIAKYCDAYVMHGDEPEHIAAKIS
                     DMKARRESFGLPPMSFGMAGYAVVRDSQAEADRELERITTIPGLADGTPPPGFANFDQ
                     WLAGTELERELKIREYSVSNRGLRPGFVGTPEAVRGRIAEFEAAGLDLLLLQMSPQEE
                     EMERFAAQIIRR"
     gene            complement(3192..4130)
                     /locus_tag="VF686_04985"
     CDS             complement(3192..4130)
                     /locus_tag="VF686_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEX8661201.1"
                     /translation="MTSPLPDLAVVYEHPQWFEPLFEALDRRGVSYVKIPLAGHVFDP
                     AGSSAPARVVFSRVAMSSFLREPEHPIFYAQSLFEHWQGQGTRVINASALPIDTSKAR
                     QLSLIARLGFKGPETRAAHRQADLPKAAEGLRFPVLVKADIGGSGSGIVRYDTAEELA
                     QAAREGTAPAGINGISLVQEYAPREGGRIIRVETLAGRYLYALDVESSGDAFDLCPAD
                     VCLIGPGSGVMGMRRFEPPAEVIAAVEAIVAAGEIEIGSVEYLIDARDGSVRFYDINA
                     LSNFVARPREVLGFDPHDNLVDWLVGIIEEEKAKRT"
     gene            4275..4359
                     /locus_tag="VF686_04990"
     tRNA            4275..4359
                     /locus_tag="VF686_04990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4309..4311,aa:Leu,seq:caa)
     gene            complement(4376..4930)
                     /locus_tag="VF686_04995"
     CDS             complement(4376..4930)
                     /locus_tag="VF686_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661202.1"
                     /translation="MRLSILAAALALACAAPAAAQTPPPPPVPVSSAAPADVATPEAI
                     VAALYDVISGDAGVARDWARFRSLFHPTARLMPSGMNREGQGVVRSVTPDEYITRSEP
                     LLVGEGFHEREIARRSERFGQIVHVWSSYESLHSLSDPRPFARGINSIQLFNDGSRWW
                     IVSVYWQAETPAAPIPAEYLPPAA"
     gene            complement(4993..5403)
                     /gene="arsC"
                     /locus_tag="VF686_05000"
     CDS             complement(4993..5403)
                     /gene="arsC"
                     /locus_tag="VF686_05000"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521993.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX8661203.1"
                     /translation="MTDFPVTVFHNPACGTSRNVLAMVEAAGYRPEVVEYLQTGWTAD
                     QLRDLFAEAGLTPRDALREKEAAATVPALLDPATPDDAILEAMLEHPVLVNRPIVRTP
                     RGAAMCRPSEKVFGLLDRRPDRFVKEDGEVVELA"
BASE COUNT          860 a         1857 c         1822 g          887 t
ORIGIN      
        1 tcgcggtgat cgtgatcctg ctgcgcaatc ggcagaagcg gaccctgaga cccgaactga
       61 tgtggatcct gcccgcgatc ttcatacccc tgatcgcatt cggcctgtgg ggcatgcgtc
      121 agacgccggg cgcggatctg acgccctatg gtcccgccga ttggggcctg ctggccggct
      181 gcgccgtgct gggcgcgctc gggggtttct ggcggggcag catggtgacc atcgagaagg
      241 acccgggcgg cgtgttgaag gcccaggcct cgccgttggg gataatcctg atcatcgttc
      301 ttctggccgt ccgctcgttc gcccgtccct ggctggaggg ccacgccgaa agcctcggcc
      361 tgcacatcgt cgcggtggaa cagggcttct tgctgctggc cggtggcctg atcgtcgccc
      421 agcgaatcga gatgttcatc cgggctcgcc ggattatggc aggcgcaccc gacccccatg
      481 tggaggtcgt ggcgtgaggg cgatcatctt cgcttttctc gccgccattg tcgccggtcc
      541 tgtcctcgcc cagggcgcag aggaggactg ggatctgagg gaagatccgc aacagcagct
      601 gacgctggcc acgctgaact tcggggacaa ccagatcgct ctgcgctgcc gggccggcgt
      661 gctggacctt ctgctcaccg gcgtgccgcc gacagacgcc gccacgcgga cggtcagggt
      721 cactgcgggc gcgatccgga acgagagcca ggtctggttc accagggcag gccaaccgat
      781 tacctcaacg ggcgaaccgg agcggctggc gcgtcaactc cgggctgggc gagaactgga
      841 tgttcttctg gaggccaccg gacccggaga acggcctcgt cgctaccgcc tgcctgttcc
      901 cgcctcggcg gtgtcggtcg accgggttct gaccgcctgc tctgttcctc tgacggacga
      961 ttgggatttg cggccgaggg cggaaccagg actgattgtc tgggatagcc agccccgacc
     1021 tgactttccg tcgcttgcta tcgagcgagg tttcgaacaa gcggaggtca ggctggcctg
     1081 ccttgtcgct gcagcaggcg ccctcgacga gtgccgcatc ctgtcggaga cgcccgctgg
     1141 agcggggttt ggacaaagcg ctcagcgatc cgccgcgcgc gcgcggctgg ggctgcccgc
     1201 agacgacgca acggtgatcg gcacggtgat cagcttcacg atccggttcc gggcttcctg
     1261 aaacgccgtc ttgacgcgcg accttcgatc cccgcagggt tgcagcacaa aacccgttcg
     1321 gattgttccc atgaagcgta tcctgtccgc ccttctggcc tgcgccgtcg cgcttccggc
     1381 gctcgcccag gatgcctccg ccgactggga tcttatgacc gatcagcgca ggaggctgac
     1441 ggtcgcctac acccgcttcg acaacggcgt gaccgtcgct acgcgctgcg ccgacggagg
     1501 gttcgaaacc atcgtcactg gcctgccgcc tgcgggcgca gcgacccgca gaacgctgcg
     1561 gatcgcgttc ggcgatgatg atctcttcga tagccagtgg agcgtcgcga ccgatgacac
     1621 tgtcgcggtg gccgatctac cggcgccgtt cgctcgccga atgcgcgctg gcggacgtct
     1681 gcgcatcctc gtccccaatg gagccacgga ggggcgcaac ctgctgtatg acctgcagct
     1741 gccggcttcg tcgggcgcca tcgaccagac gctgacatct tgcggacgcc ccctcgtcga
     1801 tccgcgcgac gccgaactga acgcgttgcc ggacgacggc gtgccaggaa ccatcgcctg
     1861 ggccgttcgg ccgagaccgc agtatcccga gacccggtat gcgcgcgggt tcgcggtcat
     1921 cacctgcctg accaacccgg acggttcatt gcgggactgc gcgatcgaat ccgaacaccc
     1981 gcacgatggg cggttcggca ggccgaccct ggcgtccgcc cgacgtgcgc gcttgcaaaa
     2041 tatcgctgag cccggcgcac cggtgcctgc ggcccaggtt ctgtataagg tcaactacgt
     2101 catggacggc taccagaccc gggaagagga gcgcgctcgc cgcccggcgc gcggctaacg
     2161 gcggatgatc tgggcggcga accgctccat ctcctcttcc tgcggcgaca tctgaagcag
     2221 cagcaggtcc agcccggcgg cttcgaactc ggcaatccgg ccccggacag cttcgggcgt
     2281 gccgacgaac cccggacgca ggccgcggtt ggagacgctg tactcccgga ttttgagctc
     2341 ccgctccagc tccgtgcccg ccagccactg gtcgaaattg gcgaagcccg gaggcggcgt
     2401 tccgtcggcc agacccggga tcgtcgtgat ccgttccagc tcgcggtccg cctcggcctg
     2461 actgtcgcgc accaccgcat agccggccat gccgaacgac atcggcggca ggccgaagct
     2521 ctcgcgccgc gccttcatgt ccgaaatctt ggcggcgata tgctcgggct cgtcgccgtg
     2581 catgacatag gcgtcgcagt acttcgcgat cagcgtcttg gccgcctcgg actcgccgcc
     2641 tgcgtatatg accggccgcg gccgcgccgg ggtcgaggtc ggcttgggct caacgatggc
     2701 gtccttcagg gagtagtagc ggccctcgaa atcggtccgg cgctgcgtcc acaggcgatc
     2761 gacgacctga agccattccg tggtccgcgc gtaacggtcg tcgtgctgat cgaactgcag
     2821 gccgtagctt tcggcctctt tcgcccacca ggacgagacg acgttcagcg ccagccggcc
     2881 gccggaaatc tgatcgatat tggccgccgc cttggcgaac agggcgggct ggtggaagtt
     2941 cggccgaacc gcgaccatca gctcgatggt cttcgtcacc gcagccagcg ccccggcgct
     3001 ggaccaggcg tccagcgccg gggcctcgat ccccttgatg tcgttcaggt tcagttcggc
     3061 gatcagggtc agatcccagc cgatctcctc cgaccgccgg accagccgcg acaggttctc
     3121 ccaggtcgcc gcacgctcgt cgtcgacgtt ccgcagccag ccgccgaaga ccggggccca
     3181 gtagccgtac ctcatgtgcg cttcgccttc tcttcctcga tgatgccgac cagccaatcg
     3241 accagattgt cgtgcggatc gaagcccagc acctcgcgcg gcctggccac gaagttcgac
     3301 agggcgttaa tgtcgtagaa ccgcaccgag ccgtcgcgcg catcgatcag atattcgacc
     3361 gagccgatct cgatctcgcc ggccgcgacg atggcctcga ccgcggcgat gacctcggcc
     3421 ggcggctcga accgcctcat acccatgacc cccgatcccg gcccgatcag gcaaacgtcc
     3481 gccggacaca ggtcgaaagc gtcgccggag ctttcgacgt ccagcgcata gagataccgg
     3541 ccggccagag tctccacccg aatgatccgc ccgccttcgc gcggtgcgta ttcctgaacc
     3601 agcgatatgc cgttgattcc cgccggcgcg gttccttcgc gcgccgcctg cgccagctcc
     3661 tcagctgtgt cgtagcggac gatgccggag cccgaaccgc cgatgtcggc cttgaccagc
     3721 acggggaagc gcaagccctc ggcggccttc ggcaggtcgg cctgccggtg cgcggcccgc
     3781 gtctcagggc ccttgaaacc gaggcgggcg atcaggctca gctgccgcgc cttcgaggtg
     3841 tcgatcggca gggccgaggc gttgatgacc cgcgtcccct gcccctgcca atgctcgaac
     3901 aggctctggg cgtagaagat cgggtgttcg ggctcgcgca ggaaggacga catcgccacc
     3961 cggctgaaca ccacccgcgc cggcgccgac gaccccgccg gatcgaacac gtggccggcc
     4021 agcgggatct tcacatagga aacgccgcgc cggtccagcg cctcgaacag gggttcgaac
     4081 cactgtggat gttcatagac gacggccagg tcggggagcg gagaagtcat gcatcggcaa
     4141 atgcgttaca cctcgatgaa acgcaagagg ggcggttgcg attcatcgaa cgaagcggac
     4201 cgcgggacgc ttgccgacga cctgccgagc ggataggtca cgtcaaccac ggcgctccgg
     4261 cgtcggcacg acacgcgggt gtggtggaat tggtagacgc gccggactca aaatccggtt
     4321 ccgaaaggag tgccggttcg accccggcca cccgcaccac cttcaaactt gagcatcacg
     4381 ccgccggcgg cagatactcc gccgggatcg gcgcagccgg ggtttcggcc tgccagtaga
     4441 cgctgacgat ccaccagcgg gagccgtcgt tgaacagctg gatgctgttg atgccgcgcg
     4501 cgaacggtcg cgggtccgac aggctgtgca ggctttcgta gctggaccag acgtggacga
     4561 tctggccgaa ccgctcggag cggcgggcga tctcgcgctc atggaagccc tcgccgacca
     4621 gcagaggctc gctgcgggtg atgtattcgt ccggcgtgac cgagcggacc acgccctgac
     4681 cctcgcggtt catccctgac ggcatcagcc gggcggtcgg gtggaacagc gaccggaagc
     4741 gggcccagtc ccgggcgacc ccggcgtcgc cggagatcac gtcgtaaagg gccgcgacga
     4801 tggcctcggg cgtggcgaca tccgccgggg ccgcgcttga caccgggacc ggcggcggcg
     4861 gcggcgtctg ggccgcggcg ggggcggcgc aggcgagggc cagcgctgcc gcgagtatcg
     4921 aaagacgcat ccgtgatcct ccctgattgg ggggactatg ccgggacgat cggcggtgcg
     4981 ccagcggcgg cgtcaggcga gctcgacgac ttccccgtct tccttgacga aacggtcggg
     5041 ccggcggtcc agcaggccaa agaccttttc ggacgggcgg cacatagccg cgccgcgcgg
     5101 ggtgcggaca atggggcggt tgaccagcac cggatgctcc agcatcgcct ccaggatcgc
     5161 gtcgtcgggc gtcgccgggt ccagcagggc cggaacggtc gccgccgcct ccttctcgcg
     5221 cagggcgtcg cgcggcgtca ggccggcctc agcgaacagg tcgcgaagct ggtccgcggt
     5281 ccagcccgtc tggagatact cgaccacctc gggccggtat cccgcggctt cgaccatggc
     5341 caggacattg cgcgacgtgc cgcaggcggg gttgtggaag acggtcacgg ggaagtcggt
     5401 catgtcccgc cgattagcat ttcctg
//
LOCUS       DASVVN010000312         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_432705,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000312 DASVVN010000000
VERSION     DASVVN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432705"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..468
                     /locus_tag="VF686_05005"
     CDS             202..468
                     /locus_tag="VF686_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEX8661204.1"
                     /translation="MIRTFNRLFVDHPREVEESYLHHMAASSRFGFRLLKLAGCAFMH
                     AMVPGVHKATVSRAVCCMAEELDGRAKEARECRMRDAGVWDPGL"
     gene            479..1660
                     /locus_tag="VF686_05010"
     CDS             479..1660
                     /locus_tag="VF686_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX8661205.1"
                     /translation="MTQGPLSGVRVLDLSRVLAGPWATQMLADLGAEVIKIERPGVGD
                     DTRHWGPPFTTKADGSKGDAAYFLCANRGKKSVELDLATPEGAAVIRELAKTCDVVVE
                     NFKTGGLKKYGLDHAALSAVNPKLVYCSITGFGQDGPDAHRAGYDYMIQAMGGLMSIT
                     GQPDGALGAEPMKVGVAVVDLFTGLYASNAILAALMHARATGQGQHVDIALFDVQAAM
                     LANQATNFFVSGKAPTRMGNAHPNLAPYQPFPCTDGMVVIAVGNDGQFRALCGALGVE
                     GMGADPRFLTNALRIEHRAVMTAELSALTAGLSMHRLMAALEAAGVPCGPVNTIDQVF
                     DEPQAKHRGLEVMQTRADLAGPVRTVASPIRMSVTPVAYDRAPPALGQDTDEVLGGLQ
                     R"
BASE COUNT          287 a          575 c          670 g          306 t
ORIGIN      
        1 gggccgcgtc ctggatcatc cgctcgtcgt cggtcaggcg gccggacagg tccagcgggt
       61 cgtcccagcg gaaggtcttg tgcgaagcgg gggcgccgtc ggccatggag agtctttcgt
      121 aagcgtggcg gaggcgcgcg gtttaggcca aaatgcaccg gtcgggtaga gggacggccc
      181 ggcgcaggtt aggctgtcgc catgatccgc actttcaatc gcctgttcgt cgaccatccg
      241 cgcgaggtgg aggagagcta tctccatcac atggcggcct cgtcgcgatt cggcttccgc
      301 ctgctgaagc tggccggctg cgccttcatg catgcgatgg tgccgggggt tcacaaggcg
      361 acggtgtccc gggcggtctg ctgcatggcc gaggagctgg acggtcgggc gaaggaggcg
      421 cgcgagtgcc ggatgcgcga cgcgggcgtc tgggacccgg gtctctgacc cagcctgcat
      481 gacccaaggc cctctttccg gcgttcgcgt cctcgatctg tcccgcgtgc tggccgggcc
      541 gtgggcgacg cagatgctgg ccgacctcgg ggccgaggtg atcaagatcg agcggcccgg
      601 cgtcggcgac gacacgcgcc actggggtcc gcccttcacg accaaggccg acgggtccaa
      661 gggagacgcc gcctattttc tgtgcgccaa caggggcaag aagtcggtcg agctggacct
      721 ggccacgccc gagggggcgg cggtcatccg cgaactggcg aagacctgcg atgtcgtggt
      781 cgaaaacttc aagaccggcg ggctgaagaa gtacggcctc gaccacgcgg cgctgtcggc
      841 ggtcaatccg aagctggtct attgctcgat caccggcttc ggtcaggacg gtcctgacgc
      901 gcaccgggcc ggctacgact acatgatcca ggccatgggc gggctgatgt cgatcacggg
      961 ccagcccgac ggcgctctcg gggccgagcc gatgaaggtc ggggtggcgg tcgtggatct
     1021 gttcaccggc ctgtacgcct ccaacgctat tctggccgct ctgatgcatg cacgggcgac
     1081 ggggcagggg cagcatgtcg acatcgccct gttcgacgtt caggcggcca tgttggccaa
     1141 tcaggcgacc aacttcttcg tctcgggcaa ggcgccgacg cggatgggca atgcgcatcc
     1201 gaacctcgcc ccctatcagc ccttcccctg caccgacggc atggtggtga tcgccgttgg
     1261 caatgacggg cagttccggg cgctgtgcgg ggcgctgggc gtcgagggca tgggcgccga
     1321 tccgcgcttc ctgaccaatg ccctgcggat cgagcatcgg gcggtgatga ccgccgagtt
     1381 gtcggccctg acggcgggcc tgagcatgca ccggctgatg gcggcgttgg aggcggcggg
     1441 ggtgccctgc gggccggtca acacgatcga tcaggtgttc gacgagccgc aggcgaaaca
     1501 tcgcgggctg gaggtcatgc agacgcgggc cgatctggcc gggccggtgc ggacggtggc
     1561 ctctccgatc cggatgtcgg tgacgccggt ggcctatgac cgggctccgc cggccttggg
     1621 gcaggatacg gatgaagtgc tgggcgggct ccagcgctag ggtcgcccgt ccgggccagt
     1681 gcaaaaacac cgtccgaagc gtccgaagcg tccactccgt ccaatcagtc cgcaccgttc
     1741 gctgcaaaaa cagagtccaa tgagtccgaa gagtccattg agtccacttg gggccggtgc
     1801 ggctgcgcag gattcagttg tcaaagaccg ggcccacg
//
LOCUS       DASVVN010000313         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_196872,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000313 DASVVN010000000
VERSION     DASVVN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196872"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1006)
                     /locus_tag="VF686_05015"
     CDS             complement(11..1006)
                     /locus_tag="VF686_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX8661206.1"
                     /translation="MAAVPETIAAVPDPEPSAAQGLEAMLDLLGEGVAVFDADDRFVL
                     WNRRYAQIYSLSQACLRAGGSYRNFLQHGAEMGQYLSGQAGKPDWLAERLRPGPKPLQ
                     EHLPGDRWVRVKDHAWNGGRVRVVTDTTGVRRNESSFKLLFEASPIPLSVVDAKTLQI
                     LAVNDAGVRYYGYSREAFLAMRTPDLAPPEGRQSMDAIMTSDIGSYQGEEVWTHVSAD
                     GRLLRIRPYVRPLVYEGRPCLLAAAVDVGAAMDAEAELRHGLDRATSASRVQAETIGL
                     IGDSLRPPLANLLQLSSALLAADLGPEVRAIAEQIRAAAGAMEGEVLQLPTGNRD"
     gene            1121..1360
                     /locus_tag="VF686_05020"
     CDS             1121..1360
                     /locus_tag="VF686_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8661207.1"
                     /translation="MPMSADEIRAHLREAFPDAEIVIQDLAGDGDHYRARVVSRAFAG
                     VARVKAHQMVYAALKGKMGGELHALALETSAPEEA"
     gene            1364..>1732
                     /locus_tag="VF686_05025"
     CDS             1364..>1732
                     /locus_tag="VF686_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661208.1"
                     /translation="MRYRPFGGGGLVVSAVSLRLKTGTSPDAVAPLMHGALEHGINYF
                     EIPAGDPEILTAAGQALRPVDRNLLVVTLRIGGALTGASRDFSPQTLANQIVDAQRRT
                     GIREFDGLVLDDPAAEELSPK"
BASE COUNT          244 a          629 c          604 g          255 t
ORIGIN      
        1 cgtcggcgcg ctagtcgcgg ttgcccgtcg gcagctgcag cacttcgcct tccatcgcgc
       61 ccgcggcggc gcggatctgc tcggcgatcg cacgcacttc ggggccgagg tcggcggcca
      121 gcaacgcgct cgacagctgc agcaggttgg cgagcggcgg gcgcaggctg tcgccgatca
      181 gcccgatggt ttcggcctgc acgcggctcg cggacgtcgc ccggtcgagg ccgtggcgga
      241 gctcggcctc ggcgtccatg gcggcgccga cgtccacggc ggcggcgagc aggcacggcc
      301 gcccttcgta gaccagcggc cgcacgtagg ggcggatgcg cagcagccgc ccgtcggcgc
      361 tgacgtgcgt ccaaacttcc tcgccttggt aggagccgat gtcggaggtc atgatcgcgt
      421 ccatgctctg gcgaccctcc ggcggcgcca ggtccggcgt gcgcatggcc aggaacgcct
      481 cgcggctgta gccgtagtag cggacgcccg cgtcgttcac ggccaggatc tgcagcgtct
      541 tggcgtcgac caccgacagc gggatggggc tcgcctcgaa cagcagcttg aacgagctct
      601 cgttacggcg gacgccggtg gtgtcggtca ccacccgcac cctgcccccg ttccacgcgt
      661 ggtccttcac ccgcacccag cggtcgcccg gcaggtgttc ctgcagcggc ttcgggccgg
      721 ggcgcaggcg ctcggccagc cagtcgggct tgcccgcctg gccgctcagg tactgtccca
      781 tctccgcgcc gtgctgcagg aagttccggt aggacccgcc tgccctgagg caggcctggc
      841 tcagcgaata gatttgcgcg taccggcgat tccacagcac gaaccggtcg tcggcgtcga
      901 agaccgcgac gccttcgccc agcaagtcga gcatggcctc gaggccctgc gccgccgagg
      961 gctccgggtc aggaacagca gcaatcgttt cgggaacggc tgccatggga ccagaatagc
     1021 gccagagggt aaacccaacg ttgccccggg gcgcctccgg cgcagggtcg taagccggat
     1081 gttaagcttg ccgggctacc cgttccgagg ggagttccgc atgccgatgt ccgccgacga
     1141 gatccgcgcg cacctgcgcg aagcgttccc ggacgctgag atcgtcatcc aggacctcgc
     1201 cggcgacggc gaccactacc gggcccgggt cgtctcccgc gccttcgccg gcgttgcacg
     1261 ggtcaaggcg caccagatgg tttatgccgc cctcaagggc aaaatggggg gcgagttgca
     1321 cgccctggcg ctggaaacca gcgcgcccga ggaggcctga gccgtgcggt accgtccctt
     1381 cggcggcggc ggcctggtcg tttccgccgt gtcgctccgg ctgaagaccg gcacctcccc
     1441 agacgccgtc gccccgctga tgcacggcgc cctcgagcac gggatcaact acttcgagat
     1501 tccggccggc gaccccgaga tcctgaccgc ggcgggccaa gccctgcgcc cggtcgaccg
     1561 caacctgctg gtggtcacgc tccgcatcgg cggcgcgctg acgggcgcgt cgcgggactt
     1621 cagcccgcag accctggcca accagatcgt cgacgcgcag cggcgcaccg gcattcgcga
     1681 gttcgacggc ctggtgctgg acgatccggc cgccgaggag ctctcgccca ag
//
LOCUS       DASVVN010000314         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_116168,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000314 DASVVN010000000
VERSION     DASVVN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116168"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF686_05030"
     CDS             complement(<1..612)
                     /locus_tag="VF686_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8661209.1"
                     /translation="MTSRVSRPLPDQIFTEARERILSGRLPADSPIRQDALAIELGVS
                     KIPLREALARLESDGLVVSHPNRGFIVRPLSRADAEDVFDLRLRIEPNACVLGALGAD
                     QAAHDAARAAFAALDAALRNGLADIGRRNREFHLALVRPSGRGVTIETVDRLLALSER
                     YVRVHLGEHRRLDRASHEHETVLCAWLERRTEVLRRETQAHIRA"
     gene            complement(615..1616)
                     /locus_tag="VF686_05035"
     CDS             complement(615..1616)
                     /locus_tag="VF686_05035"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEX8661210.1"
                     /translation="MARHVFRCIDGHTAGNPVRLVIEGAPALTGDTMSARRQDFLARY
                     DWIRTGLCFEPRGHDMMSGGFLYPPTTPEADVGILFIETSGCLPMCGHGTIGIVTFAL
                     ENGLITPRTPGKVIAEVPAGVIELDYVQDGGRVRSVRIRNVASWLDCQGIEIEVPDFG
                     PLAVDVAYGGNFYAIIEPQGAYAGLDDLGAARILQLSPVIRSLVREAFTPVHPLDPTI
                     RGVSHVLWADKPRGKGADGRNAVFYGDKAIDRSPCGTGTSARLAHLHATGRLPVDQAF
                     VHESYIGSRFIGRVEAESEVGGRPAIIPSVEGSAIATGLNEIWIDDEDVFAHGFQVV"
     gene            complement(1619..>1977)
                     /locus_tag="VF686_05040"
     CDS             complement(1619..>1977)
                     /locus_tag="VF686_05040"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX8661211.1"
                     /translation="GDRYVLFAGLDDVALEGLMLGARGWVSGLTNAFPKESLALWDAF
                     KRGDMEKALAIYRWFMPLLHLDADHDLVQSIKLAEQIMGRGHERVRPPRHPLVGARRA
                     EVVAMVEKAAATRPVL"
BASE COUNT          316 a          648 c          724 g          289 t
ORIGIN      
        1 ggcgcggatg tgggcctgag tctcgcggcg aagaacctcg gtccggcgct cgagccaggc
       61 gcagagcacc gtttcgtgct cgtggctggc gcggtccagc cgccggtgct ccccgaggtg
      121 cacgcgcaca taacgttcgg acagggccag cagccggtcg accgtttcga tggtgacgcc
      181 ccgacccgac ggccggacca gcgccagatg gaattcgcgg ttgcgtcgtc cgatgtcggc
      241 aagcccgttg cgaagggcgg cgtcgagggc cgcgaaggcc gcgcgggcgg cgtcatgcgc
      301 ggcctgatcc gccccgagcg cgcccagcac gcaggcgttc ggctcgatgc gcaggcgcag
      361 gtcgaagacg tcctcggcgt cggcgcgtga cagcggacgg acgatgaaac cccggttggg
      421 atgggagacg accagtccgt cgctctccag ccgggccaga gcctcgcgca gcggaatctt
      481 gctgacgccc agttcgatcg cgagggcgtc ctgacggatc ggcgagtcgg cgggcaggcg
      541 gccggacagg atgcgttcgc gggcctctgt gaagatctga tcgggcaggg ggcgcgagac
      601 gcggctggtc atcctcagac cacctggaag ccgtgggcga agacgtcctc gtcatcgatc
      661 cagatctcgt tcagaccggt ggcgatggcc gacccctcga ccgaggggat gatggccggc
      721 cggccgccga cctcggactc ggcctccacg cgaccgatga agcggctgcc gatatagctt
      781 tcgtggacga aggcctgatc gaccggcagg cggccggtgg cgtgcagatg ggccagccgc
      841 gcagaggtgc cggtgccgca ggggctgcga tcgatggcct tgtcgccata gaagacggcg
      901 ttgcgtccgt cggcgccttt tcccctcggt ttgtcggccc agagtacatg ggagactccg
      961 cggatggtcg gatcgagcgg atggaccggg gtgaaggcct cgcgcaccag tgagcggatg
     1021 acgggcgaca gctgcaggat gcgggcggcg ccgaggtcat ccagccccgc ataggcgcct
     1081 tgcggctcga tgatggcgta gaaattgccg ccataggcga cgtcgaccgc cagcggcccg
     1141 aagtcgggaa cctcgatctc gatcccttgg cagtccagcc acgaggcgac attgcggatg
     1201 cgaaccgacc ggacgcggcc gccatcctgc acatagtcga gttcgatcac gcccgcgggg
     1261 acctccgcga tgacctttcc gggcgtgcgc ggcgtgatca gaccgttttc cagcgcaaag
     1321 gtgacgatgc cgatggtccc gtggccgcac atcggcaggc agccgctggt ctcgatgaac
     1381 aggatgccga cgtcggcctc aggcgtggtc ggcggataga ggaagccgcc gctcatcatg
     1441 tcgtggccgc gcggctcgaa gcagaggccg gtgcggatcc agtcgtagcg ggcgaggaag
     1501 tcctgccgcc gggcactcat ggtgtcgccg gtcagcgccg gcgcgccctc gatcaccagc
     1561 cgcaccggat tgcccgcggt gtggccgtcg atgcagcgga agacgtggcg cgccatggct
     1621 aaaggaccgg ccgcgtcgcg gcggccttct cgaccatggc cacgacctcg gcccggcggg
     1681 cgccgaccag cgggtggcgc ggcgggcgga cccgctcatg gccccggccc atgatctgtt
     1741 cagccagctt gatcgactgg accagatcgt ggtcggcgtc gaggtggagc aggggcatga
     1801 accagcggta gatggcgagc gccttctcca tgtcgccgcg cttgaaggcg tcccacaggg
     1861 cgagggactc tttcgggaag gcgttggtca ggcccgagac ccatccgcgg gccccgagca
     1921 tcaatccctc aagcgccacg tcgtccagcc ccgcgaacag cacatagcgg tcgccga
//
LOCUS       DASVVN010000315         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_483924,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000315 DASVVN010000000
VERSION     DASVVN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483924"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1075)
                     /gene="hemB"
                     /locus_tag="VF686_05045"
     CDS             complement(74..1075)
                     /gene="hemB"
                     /locus_tag="VF686_05045"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8661212.1"
                     /translation="MTQPFAPAAFPYARPRRLRSSPWVRRLVAETTLTPADLIWPLIV
                     HDGAEERIPVASMPGVFRLSPKAAAAAAVEARDLGIPVVALFPNVDGAIKDAVGTGAT
                     DPDGLIPACIKAIKDAAPEIGVMTDVALDCYTDHGHDGVVENGRILNDPSLERLAEQA
                     FIHAHAGADIVGPSDMMDGRVQAIREALEANGFHDTLILSYAAKFASAFYGPYRDAVG
                     SAKQLTGDKKTYQMDYANSDEALREVAMDIAEGADAVMVKPGMPYLDIIQRVVETFKV
                     PTFAYQVSGEYAMMQASIANGWLDEDRAILETLHGFKRAGCAGVLSYFAPQAARLLG"
     gene            1347..>1614
                     /locus_tag="VF686_05050"
     CDS             1347..>1614
                     /locus_tag="VF686_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8661213.1"
                     /translation="MDFALNDDQRAIQDAARQFAEAELAPHSAEWDETKHFPVDVMRH
                     AAEMGFCGIYTGEEHGGMALGRVEAAVIFEELSRGDVSTAAFISI"
BASE COUNT          269 a          497 c          594 g          254 t
ORIGIN      
        1 acaacgccct gaaatcggcg gtcccggaca atgtagacgt cgcctacgac ggctggacgg
       61 tgcgactgcc gctctagccc agcaatcgcg ccgcctgcgg ggcgaaatag ctgagcacgc
      121 cggcgcagcc cgcgcgcttg aagccgtgga gggtttcgag gatggcgcgg tcctcgtcga
      181 gccagccatt ggcgatggaa gcctgcatca tcgcgtattc gccggacacc tgataggcga
      241 aggtcgggac tttgaatgtc tcgacaaccc gttgaatgat atcgagatat ggcatgccgg
      301 gcttgaccat gacggcgtcg gcgccctcag cgatgtccat ggcgacctcg cgcagggcct
      361 cgtccgaatt ggcgtagtcc atctggtagg tcttcttgtc gccggtcagc tgtttcgccg
      421 agccgacggc gtcgcggtag gggccgtaga aggccgaggc gaacttcgcc gcgtaggaca
      481 ggatcagggt gtcgtggaag ccgttggcct ccagcgcctc gcggatggcc tggacgcggc
      541 cgtccatcat gtcggagggg cccacgatat cggcgccggc gtgggcgtgg atgaaggcct
      601 gttcggcgag gcgttcgaga ctggggtcgt tgaggatgcg gccgttctcg accacgccgt
      661 cgtggccgtg gtcggtgtag cagtccagcg ccacgtcggt catgacgccg atctccggcg
      721 ccgcgtcctt gatggccttg atgcaggccg ggatcaggcc gtcggggtcg gtggcgcccg
      781 tgccgaccgc gtccttgatg gcgccgtcga cgttggggaa gagggcgacg acggggatgc
      841 cgagatcgcg ggcctcaacg gcggcagccg cggcggcctt gggcgacagg cggaagacgc
      901 cgggcatgga ggcgaccgga atgcgctcct cggcgccgtc atggacgatc aggggccaga
      961 tcaggtcggc gggcgtcagc gtcgtctcgg ccaccagccg gcgcacccac gggctgctgc
     1021 gcaggcggcg ggggcgggcg taggggaagg cggcgggggc gaacggctgg gtcatgcagg
     1081 gctcataggc ggcttggccg tccgggaaaa cagggtgcga agcgtttgaa gagtccggaa
     1141 cggtcggttc caaggcgaaa aacacagtct acagcgtcta cagcgtctac tccgtccact
     1201 tctccttccg tcgacagcgg cgatcggcaa tcttgcatgc acctacgtca gaaacggacg
     1261 tagcctcgag ggtgacgccg cgcgaggggt tggatagaag caccgcgagc ggcccggcag
     1321 aggccgacca cgacccagga gcttccatgg acttcgccct caacgacgac cagcgcgcca
     1381 tccaggacgc cgcccggcag ttcgcggagg cggaactggc tccgcacagc gccgagtggg
     1441 atgagaccaa gcatttcccc gtcgacgtca tgcgccacgc ggccgagatg ggcttctgcg
     1501 gcatctacac cggcgaagag cacggcggca tggcgctggg ccgcgtcgag gcggcggtga
     1561 tcttcgagga gctgtcgcgc ggcgacgtgt cgacggcggc cttcatcagc atcc
//
LOCUS       DASVVN010000316         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_11598, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000316 DASVVN010000000
VERSION     DASVVN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11598"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF686_05055"
     CDS             <1..214
                     /locus_tag="VF686_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169036.1"
                     /note="YbhB; similar to rat and human kinase inhibitory
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HEX8661214.1"
                     /translation="EGRTDFGEPGFGGAAPPPGHGPHRYIFTVFAVDMDKLDVTGDDS
                     GAKYGFNLHFHTLAKASITGIYENRG"
     gene            266..928
                     /gene="purQ"
                     /locus_tag="VF686_05060"
     CDS             266..928
                     /gene="purQ"
                     /locus_tag="VF686_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX8661215.1"
                     /translation="MSAAVIVFPGSNCDRDCKVAVERSTGETVQMVWHQETELPSGLD
                     LIVLPGGFSYGDYLRCGAMAVQSPIMQAVKQAADKGVAVLGICNGFQILCEAGMLPGA
                     LLRNAGLKYVCKPVSLTVTNGQTRFTAGYQGQREVIMTQGNGDGNFFADAETLNRIEG
                     EGQVVFRYVDNPNGSVNDIAGLQNARGNVLGMMPHPDRAFEEELGSADGATLFRSIYA
                     AA"
     gene            944..1489
                     /locus_tag="VF686_05065"
     CDS             944..1489
                     /locus_tag="VF686_05065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX8661216.1"
                     /translation="MTERQKMVAGEPYDPGDSELQADQAAAKQWMVRYNAALGSSPSD
                     RLVLLRERLGAVGDGAVIRPPFHCDYGYNIRLGRGVFLNFNCVILDVCEVVIGDQTQI
                     GPGVQMLTADHPRAPEQRAAGIEFGRPIRIGRNVWIGGGALILPGVTVADDAIIGAGS
                     VVTRDVPAGATVMGNPARVKA"
     gene            complement(1486..>2130)
                     /locus_tag="VF686_05070"
     CDS             complement(1486..>2130)
                     /locus_tag="VF686_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8661217.1"
                     /translation="PGLPQIQFQPRETLPWMAAVEEAAADIGIELDALLAEPDLFRPY
                     VEPRADRPNTDSKGMASNADWSALFLWKDGEEQPDMARRCPRTIKALAEVPLCRVPGR
                     TPSILFSRLKAGARIPPHHGLINTRLICHLPLIAPPGSRFRVGNDVREWKEGQGWAFD
                     DTIEHEAANDSGHDRTILIFDVWKPEVTEEERALVSAMFQAIDGYGAGGKAWGV"
BASE COUNT          365 a          712 c          716 g          337 t
ORIGIN      
        1 cgagggccgg accgacttcg gcgaacccgg cttcggcggg gccgcgccgc cgcccggcca
       61 cggcccgcac cggtacatct tcaccgtctt cgccgtggat atggacaagc ttgacgtcac
      121 gggcgatgac agcggcgcca aatacggctt caacctgcat ttccacacgc tggccaaggc
      181 gtcgatcacg ggaatctacg agaacagggg ctgaggcgac ggccacgcct gacaaggcga
      241 ccgaacgctg ctaaagggcg ccgccatgag cgcagccgtc atcgtcttcc ccggttccaa
      301 ctgcgaccgc gactgcaagg tcgccgtcga acgctcgacg ggcgagaccg ttcagatggt
      361 ctggcaccag gagacggagc tcccctccgg cctggacctg atcgtgctgc cgggcggttt
      421 ctcctacggc gactatctgc gctgcggggc catggcggtg cagtcgccga tcatgcaggc
      481 ggtcaagcaa gccgctgaca agggcgtggc cgttctgggc atctgcaacg gcttccagat
      541 tctgtgcgag gccggaatgc tgccgggcgc cctgctgcgc aacgccggcc tgaaatacgt
      601 ctgcaagccc gtcagcctga ccgtgaccaa cggccagacc cgtttcaccg ccggttatca
      661 gggtcagcgc gaggtgatca tgacccaggg caatggcgac ggaaacttct ttgccgacgc
      721 agagaccctg aaccggatcg agggcgaggg gcaggtggtg ttccgatatg tcgacaatcc
      781 caacggttcg gtgaacgaca tcgccggcct gcagaacgcg cgcggcaacg tgctggggat
      841 gatgccgcac ccggaccggg ccttcgagga agagctgggc tcggccgacg gcgcgaccct
      901 gttccggtcg atctacgccg ccgcctgagc cgagggctcg gcgatgaccg aacgtcagaa
      961 gatggtcgcc ggcgagccct atgatccggg cgattccgaa ttgcaggccg atcaggccgc
     1021 cgccaaacaa tggatggtcc gctacaatgc ggccctcgga tccagcccct ccgatcggct
     1081 tgtcctgcta cgcgaacgat tgggcgcggt cggcgacggc gcggtgatcc gcccaccgtt
     1141 ccattgcgac tacggctaca acatccggct ggggcgcggc gtctttctga acttcaactg
     1201 cgtgattctg gacgtctgcg aggtcgtcat cggcgaccag acccagatcg ggccgggcgt
     1261 acagatgctg acggcggatc atccgcgcgc accggaacag cgggcagcgg ggatcgaatt
     1321 cggcaggccg atccggatcg gccggaatgt ctggatcggc ggcggcgccc taatcctgcc
     1381 gggcgtcacg gtggccgacg acgccatcat cggcgcgggg tccgtggtaa cccgcgacgt
     1441 tcccgccggg gcgacggtca tgggcaaccc cgcccgcgtg aaggcctaga ccccccaagc
     1501 cttgccgccc gcgccataac cgtcaatcgc ctgaaacatg gcggacacga gggcgcgctc
     1561 ctcttccgtc acctcgggtt tccagacatc gaagatgagg atggtgcggt cgtgaccact
     1621 gtcgttggcg gcctcgtgct cgatggtgtc atcgaaagcc cagccctggc cctccttcca
     1681 ttcgcggaca tcgttgccga cccggaagcg cgaccccggc ggcgcgatca gcggaaggtg
     1741 acagatcagg cgggtgttga tcaggccgtg atgcggcggt atccgcgcgc cggccttcag
     1801 acgcgagaac agaatggacg gcgtccggcc cgggacgcgg cacagcggaa cctcggccaa
     1861 ggccttgatc gtgcgcggac accggcgggc catgtccggc tgttcctcgc cgtccttcca
     1921 cagaaacagg gcgctccagt cggcattgga ggccatgcct tttgaatccg tgttcggccg
     1981 gtcggcgcgc ggttcgacat agggacggaa caggtccggc tcggccagca gggcgtccag
     2041 ttcgatcccg atgtccgccg ccgcctcctc gaccgccgcc atccagggca gggtctcgcg
     2101 cggctggaac tggatctgcg gcaggccggg
//
LOCUS       DASVVN010000317         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_247747,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000317 DASVVN010000000
VERSION     DASVVN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247747"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VF686_05075"
     CDS             complement(<1..626)
                     /locus_tag="VF686_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEX8661218.1"
                     /translation="MRDLPEELTARIESGAATLCHAWLLRRTDGIETGFTDHDRDLTV
                     EGVSCRAASGWVAGAADSAVGLSAGSAALAGALDDATITGEDVAAGLYDGAGVTLWRV
                     DWTRPELGVRLWTATLARIRREGASFVAELEGPLAELERVVGRTYGRVCDALLGDARC
                     GVDLTAFPGLECDKRWATCVDSFANGINFQGFPDIPGDDFLTAAPIEGG"
     gene            complement(629..1258)
                     /locus_tag="VF686_05080"
     CDS             complement(629..1258)
                     /locus_tag="VF686_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEX8661219.1"
                     /translation="MSFHEVRLPARLAFGSTGGVERRTEIVTLGSGFERRSTPWAQGR
                     RRYLIGANLRSLDDMAVLTSFFEARRGRLYGFRFRDFADFKSCAPGASMSPTDQALGQ
                     GDGARTVFALTKRYGDDETAAERRIAKPVEDTVRVAVSGIEWPEGTFTVDPVTGMVTL
                     GEAPGAGAVITAGFEFDTPVRFDTDRIEVTLENFAAGRMAAAPLIEVRV"
     gene            1370..1855
                     /locus_tag="VF686_05085"
     CDS             1370..1855
                     /locus_tag="VF686_05085"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX8661220.1"
                     /translation="MSFENSSQIQSFDWQKLLLSFEGRTRRSHFWIGWLICLGAGVVA
                     GWIPLIGGLISLALIWPNLAITVKRLHDMGKSGWFAAIPWVVSIVGVFVGIAMVGVSA
                     FTNHAALDREDPAAMFALIGPAFGVLGLVLLVNLAFLLWIGLTDSQPGDNQFGPNPKG
                     L"
     gene            complement(1871..2401)
                     /locus_tag="VF686_05090"
     CDS             complement(1871..2401)
                     /locus_tag="VF686_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEX8661221.1"
                     /translation="MTDSLEPSGLDAVPLKAAEAAAALEGLREPAERAAASIEDAFGR
                     AGESLTRSLARAAADGEVTLAELARAMLAAVNAASGSGRGGGLSDAIAQAVQTIFSGS
                     RADGGPVAGGGAYLVGERGPEVFRPSSGGVVEPVGGAGVIVNVRVDGGAQGLLRSEGQ
                     IAQMLARAVSLGARKF"
     gene            complement(2394..2570)
                     /locus_tag="VF686_05095"
     CDS             complement(2394..2570)
                     /locus_tag="VF686_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEX8661222.1"
                     /translation="MKTPWSAMMRTAAILGTPPTGFWRLSLKEWRMLTGGPANAAPMG
                     RRELMRMAEAWPDD"
BASE COUNT          433 a         1055 c          790 g          457 t
ORIGIN      
        1 ccgccctcta tcggggcggc tgtcaggaag tcgtcgcccg ggatgtccgg aaatccctga
       61 aaattgatcc cgttggcgaa gctgtcgaca caggtcgccc agcgcttgtc gcactccaaa
      121 cccggaaagg cggtcagatc gaccccgcag cgagcatcgc ccagcagcgc gtcgcacacg
      181 cgtccatacg tccgcccgac gacccgctcc agttccgcca gcggaccctc cagttcggcg
      241 acgaagctcg caccctctct ccggattcgc gccaaggtcg ccgtccacag gcgcaccccc
      301 aattcgggcc gggtccagtc cacccgccac agcgtgacac cggcgccgtc gtataagccc
      361 gccgccacgt cctcgcccgt gatcgtcgcg tcatccaagg ctccggctaa cgccgcggat
      421 ccggcgctca gtcccacggc gctgtctgcc gcgcccgcga cccagccgct tgccgcccgg
      481 cacgaaactc cctcgacggt caggtcgcgg tcgtggtccg tgaatcccgt ttcgatcccg
      541 tccgtccgcc gcaagagcca ggcgtggcac agcgtcgctg caccgctttc gattcgggcg
      601 gtcagttcct ccggtaagtc gcgcatggct agactcgcac ctcgatgagc ggtgcggcgg
      661 ccatccttcc cgccgcaaag ttctccaggg tgacctcgat ccggtcggtg tcgaaccgca
      721 caggcgtatc aaactcgaag cccgccgtga tgacggcgcc agcacccgga gcttcgccca
      781 gcgtgaccat tccagtgacg ggatcgaccg tgaacgttcc ttcaggccac tcgattccgc
      841 tgaccgccac tcgcaccgta tcctcgacgg gcttggcgat ccgccgctcc gccgcggtct
      901 cgtcgtcgcc ataccgcttc gtcaaggcga acacggtccg tgcgccgtcc ccctgcccca
      961 gagcctgatc cgtcgggctc atcgacgccc ccggcgcaca ggatttgaag tcggcgaaat
     1021 cccggaacct gaagccatag agccttcccc gacgcgcctc gaagaagctg gtcaggaccg
     1081 ccatatcgtc cagggatcgc agattggccc cgatcaggta gcgccgccgg ccttgggccc
     1141 aaggcgtcga acgccgctcg aaccctgatc cgagcgtcac gatctcggtg cgccgttcca
     1201 cgccgccggt cgatccgaac gccagccgcg cgggcaacct tacctcgtga aaggacattc
     1261 ccactctcct gtccggtttc tgttcaagct cgctttcgct tgtcgagcga acgccgttga
     1321 gccatcctct ctccgtcgga ccaaggtccg gatcgccggg gagggcgcca tgagcttcga
     1381 aaattcatcc cagattcaaa gcttcgactg gcaaaagctg ctgctttcgt ttgagggccg
     1441 aacgcgccga tcacacttct ggatcggttg gctgatctgt ctgggcgccg gcgtggtcgc
     1501 cggctggata ccgcttatcg gcggcctgat ttccctggcc ctcatctggc cgaatctcgc
     1561 catcacggtg aagcggcttc acgacatggg caaaagcggc tggttcgcgg ccattccctg
     1621 ggtcgtcagc atcgttggcg ttttcgtcgg catcgccatg gtcggcgtct cggccttcac
     1681 caatcacgct gcactcgatc gcgaagatcc ggccgccatg ttcgcgctga tcggtcccgc
     1741 ctttggcgtg ctgggactgg tgttgctggt caaccttgcc ttcctgctgt ggatcggtct
     1801 caccgacagc cagccgggcg acaaccagtt cggtcccaac cccaagggtc tctgatcgtg
     1861 aaggcctcgt tcagaacttg cgcgcgccca gcgacacggc gcgcgcaagc atctgggcaa
     1921 tctgtccttc cgaccgcaac agtccctggg cgccgccgtc gacgcgaaca ttgacgatca
     1981 cgcccgcccc gccgaccggc tcgaccacgc ccccgcttga aggccgaaac acctccggcc
     2041 cgcgctcgcc gaccagatag gcgccgccgc ccgcgacggg cccgccatcc gcgcgcgatc
     2101 ccgaaaagat cgtctgcacc gcctgcgcga tggcgtccga cagcccgccc ccgcggcccg
     2161 atccggacgc cgcattcacc gccgccagca tggcccgcgc cagctcggcc agggttacct
     2221 ctccgtccgc cgccgcccgc gccagcgacc gcgtcagact ctccccggcc cggccgaacg
     2281 cgtcctcgat tgatgcggcc gcccgttcgg cgggctcgcg caatccttcc agcgccgcgg
     2341 ccgcttcagc tgccttcagc ggcacagcgt ccagaccgga cggttccaaa ctgtcagtca
     2401 tccggccacg cctccgccat tcgcatcaat tcgcggcgtc ccatcggcgc cgcgttcgca
     2461 ggcccgccgg tcagcatccg ccattccttc agcgagagcc gccagaatcc tgtcggcggg
     2521 gtgcccagga tcgcagccgt tcgcatcatc gcgctccagg gcgtcttcac gacgccgccg
     2581 cgaaggcctt ggccaccgcc tctgccgcct cacggggatc gatcgcggca cgaccgatat
     2641 ccgccgccag ctcaaactct ccacccccgc gcagcagagc cgccagaaca gccataaggt
     2701 cgcggccgga gagcgcccgc atccgctccg gcaag
//
LOCUS       DASVVN010000318         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_196932,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000318 DASVVN010000000
VERSION     DASVVN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196932"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF686_05100"
     CDS             complement(<1..664)
                     /locus_tag="VF686_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8661223.1"
                     /translation="MLRIARTEIAAVSALFIVALGVMTFVEVADDMTEADGQAFDHAV
                     LAWVQPAAGEPRGPWWLREAAADLTSLGGISVLALFAVIAFAFLLIQRKQLSALLLVV
                     GLAGGVALSEGLKAMFGRERPPADFQAVDTLNASFPSGHALLSTVFYLTLGVMLTRAF
                     PRAHLKAFVLGAALLIALLIGLTRVYLGAHWATDVFAGWCAGAAWAMALWLVAYAIGR
                     RQA"
     gene            969..>1700
                     /locus_tag="VF686_05105"
     CDS             969..>1700
                     /locus_tag="VF686_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX8661224.1"
                     /translation="MAGGEALKTVDFVSPTEGDAAARPHLSVVRSIQLDRSRDDLLTD
                     FGKKTLEDRYLLAGESYQDMFARVATAYADDADHAQRVYDYMSKLWFMPSTPVLSNGG
                     ADRGLPISCFLNAVADSLDGIQSVWNENVALASNGGGIGTYWGGVRSIGEKVKGAGQT
                     SGIIPFIRVMDSLTLAISQGSLRRGSAAVYLDVHHPEIEEFLEIRKPSGDFNRKSLNL
                     HHGISVTDEFMEAVRDGVPFGLRSPK"
BASE COUNT          302 a          560 c          562 g          276 t
ORIGIN      
        1 cggcctgccg tcgcccgatc gcataggcca caagccacag cgccatggcc caggccgcgc
       61 cggcgcacca gcccgcgaac acatccgtcg cccagtgggc gccgagatag acccgcgtca
      121 gtccgatcag cagggcgatc agaagcgccg cccctagcac gaacgctttc agatgggcgc
      181 gggggaaggc ccgggtcagc atgacgccaa gggtcagata gaagacggtc gacagcaggg
      241 cgtggccgga cggaaagctg gcgttcagcg tgtccaccgc ctgaaaatcg gcgggaggcc
      301 gttcccggcc gaacatggcc ttcagcccct cgctgagcgc gacgcccccg gccagaccca
      361 cgaccagaag cagggcggac agctgtttgc gctggatcag caggaaggcg aaggcgatca
      421 ccgcgaacag ggccagcacc gatatgccgc ccagcgaggt caggtcggcc gccgcctccc
      481 tgagccacca cggcccccgg ggctcgcccg cagccggctg cacccaggcc agcacggcgt
      541 gatcgaaggc ctggccgtcg gcttcggtca tgtcgtcagc cacctcgacg aaggtcatga
      601 cgccgagggc gacgatgaac agggccgaga ccgccgcgat ttccgtccgg gcgatacgca
      661 gcatgcgacg gaaaaacgtg atcgtctggt cggtggtcat cggtcgctaa cggtcccgct
      721 tgtgtgacgt tccactcacg cgttcgtgaa cggaatacgg tgaggcttgt cacgccgtcg
      781 tcgcggcctg tccacaggct catcccccgt cttgaggccg attcgggcag aaagtgatcg
      841 tcaaccccgt tgacggttgg ttagccatct gcgccccata tgtgggcttg gggagcgctt
      901 cagccactag gtcttgtggt tcgaagcgcg gggacaccca ttcgattttg ttcagggcgg
      961 cggatatcat ggctggcggc gaagcgttga agacggttga tttcgtgtcc ccgacggagg
     1021 gtgatgcggc ggcgcgtccc catctgtccg tggtgagatc cattcaactc gaccggtccc
     1081 gtgacgatct gctgaccgac ttcggcaaga agacgctgga ggatcgctat ctgctggccg
     1141 gcgagagcta tcaggacatg ttcgcccgcg tcgccacggc ctatgccgat gacgcagacc
     1201 acgcccagcg cgtctacgac tacatgtcga agctgtggtt catgccgtcg acgccggtgc
     1261 tgtcgaacgg cggcgccgat cgcggcctgc cgatcagctg cttcctcaac gctgtggccg
     1321 acagcctgga cggcatccag tcggtgtgga acgagaacgt cgccctggcc tcgaacggcg
     1381 gcggcatcgg cacctactgg ggcggcgtcc ggtcgatcgg cgagaaggtc aagggcgcgg
     1441 ggcagacgtc gggcatcatc cccttcatcc gggtgatgga ctccctgacg ctggcgatca
     1501 gccaggggtc gctgcgccgt ggttcggccg ccgtgtacct cgatgtacac catccggaaa
     1561 tcgaggagtt cctcgagatc cgcaaaccct cgggcgactt caaccgcaag tccctgaacc
     1621 tgcaccacgg catttccgtc accgacgagt tcatggaggc ggtgcgcgac ggcgtgccct
     1681 tcggcctgcg ttcgcccaag
//
LOCUS       DASVVN010000319         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_356114,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000319 DASVVN010000000
VERSION     DASVVN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356114"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..473)
                     /locus_tag="VF686_05110"
     CDS             complement(261..473)
                     /locus_tag="VF686_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8661225.1"
                     /translation="MATGTVKWFNSTKGYGFIQPDDGGKDVFVHISAVEQAGLRGLDE
                     NQKVSYEIERDKRSGKESAGQLKTEG"
     gene            complement(637..849)
                     /locus_tag="VF686_05115"
     CDS             complement(637..849)
                     /locus_tag="VF686_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661226.1"
                     /translation="MSNHRPTTLERAYELARAGGCRTVGDIKTALQAEGFERIQDSLY
                     GPTLTAALRKLCQEHYVAAPEGEAAE"
     gene            complement(888..1595)
                     /locus_tag="VF686_05120"
     CDS             complement(888..1595)
                     /locus_tag="VF686_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX8661227.1"
                     /translation="MIRTLTTVAAAATAFLAFAGVAQAQDAAPAAGSWSFAVGAATDN
                     RSKEASKSNGEAYVWGEAEWESASGLFYAGTGLETIKSSTGSELEGKIGAGFTPDVAG
                     FDLNLNATFKQQFEANPGADDDAWEFTADVSRSIGPASGRIRLQHSPDGTGSTEAWTW
                     YEARIGWDFTNKLEATASIGRREQDNSIDYTGWNAGFTYALTDKLEADVRYHATDADV
                     PGEQYADAFVVGLSIAF"
     gene            complement(1627..2553)
                     /locus_tag="VF686_05125"
     CDS             complement(1627..2553)
                     /locus_tag="VF686_05125"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX8661228.1"
                     /translation="MAIPDSLRKGLKLPVIAAPMFLVSGPDLVVEACNAGVIGTFPSL
                     NQRTTEGYRDWIHEIKARLTPDAAHWGVNHIVHPTNPRLMADMMVTVEEKAPLVITSL
                     GAVRDVVEAVHGYGGVVFHDIANVRHARKAAQAGVDGLILVANGAGGHAGVVNPFALV
                     DEVRSFFDGTIILSGCLSTGSDVAAALMMGADFAYMGTRFISTTESGAQPEYKQMIVD
                     AGASDITYTPAVSGIPANFLTPSLIANGIDPKSLPEHKLDMGDEARAWKTVWSAGQGA
                     GAVHDILPTAQLVRRLTDEFSEACNKFDNNRL"
     gene            2766..3227
                     /gene="queF"
                     /locus_tag="VF686_05130"
     CDS             2766..3227
                     /gene="queF"
                     /locus_tag="VF686_05130"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX8661229.1"
                     /translation="MTTDTSGLHQLGQHTVQPASPETAELERVPNPHAGTLYLTRFTA
                     PEFTSLCPVTGQPDFAHIVIDYAPGDWLVESKSLKLYLTSFRNHGAFHEDCTVAIGKK
                     IADLLQPKWLRIGGYWYPRGGIPIDVFWQTGPAPEGLWLPDQGVPTYRGRG"
     gene            3339..>3700
                     /locus_tag="VF686_05135"
     CDS             3339..>3700
                     /locus_tag="VF686_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661230.1"
                     /translation="MVSKLRRGVLIILWFWFASVLATAQTISPPQTIEPAASNEQVLR
                     EDSGFSPATKRTATRGAAAFRAPSLETVIQGLGVFVAAAAAVFSAISARGSVAAANEM
                     RQARRDATKPRLSYRVLDR"
BASE COUNT          612 a         1149 c         1263 g          676 t
ORIGIN      
        1 cggcgagggc cgccatcagc gcggtgcggg tctggatcag tttcattggc ttttctcctg
       61 agcggccgtc gatacggctc gccccagaga cggcggcagg ggcgaggcgg atgcggggcc
      121 cgggcgaact ggcgtctggt cacggcgtga aactggaaga caccgtgaaa cgtagcgggc
      181 ccgtaaacga aaaacggcgg cgcgggcttt cgcctgcgcc gccgtcgaaa tcatgcgtcg
      241 gtaaagcccg acgcgtgaac tcagccttcg gtcttcagct gaccagccga ttccttgccc
      301 gaacgcttgt cgcgctcgat ttcgtaggaa accttctggt tttcatccag gccgcgcagg
      361 cccgcttgct cgacggccga gatgtggacg aagacgtcct tgccgccgtc gtcaggctgg
      421 atgaagccgt agcccttggt ggagttgaac catttaacag tgccggtagc cattttcagg
      481 acctccgtta gcgttggctt cagggaagag cccctggagc ctgtcgggtc tgaatggagc
      541 ggctagacct cagtgcgaaa gcagagggtg gcgtttcgca gagagatcga caaaacgctt
      601 ttgacgcgtc gtcgcggcga aatcaagccg caaggcttac tcggccgctt cgccctcggg
      661 ggcggcgaca tagtgttcct ggcacagctt gcgcagggcg gccgtcagcg tggggccgta
      721 cagggagtcc tggatgcgct cgaaaccttc ggcctgaagc gcggtcttga tgtcgccgac
      781 ggtgcggcag cccccggcgc gcgccagctc ataggcgcgt tcgagggtcg tgggacggtg
      841 gttgctcatt gctcctagat aggagctttg ccgcaaaaag cgaggtttta gaacgcgatg
      901 ctcagtccga cgacgaaggc gtccgcatat tgctcgcccg gcacgtcggc gtcggtggcg
      961 tgatagcgca cgtcggcttc cagtttgtcg gtcagggcgt aggtgaagcc cgcgttccag
     1021 ccggtgtagt cgatgctgtt gtcctgctcg cggcggccga tggaggcggt ggcttccagc
     1081 ttgttggtga agtcccagcc gatacgggcc tcgtaccagg tccaggcttc tgtcgagccc
     1141 gtgccgtccg gcgagtgctg caggcgaatg cggccgctgg cgggtccgat ggaccgcgag
     1201 acgtcggcgg tgaactccca ggcgtcgtcg tccgcgcccg gattggcctc gaactgctgt
     1261 ttgaaggtgg cgttcagatt caggtcgaag ccggcgacgt cgggcgtgaa gcccgcgccg
     1321 atcttgcctt ccagttcgga gccggtggag ctcttgatgg tttcgaggcc ggtcccggcg
     1381 taaaacagcc ccgaggcgct ctcccattcg gcctcgcccc agacataggc ctcgccgttc
     1441 gacttggagg cctctttcga gcggttgtcg gtcgcggcgc cgacggcgaa cgaccacgat
     1501 ccggcggcgg gcgcggcgtc ctgggcctga gccacaccgg cgaaagcgag gaaggcggtc
     1561 gccgcggcgg cgacggtcgt cagggtgcgg atcatgggat tcggcctcgt gggaacgggc
     1621 cggggtctag aggcggttgt tgtcgaattt gttacaggct tcggaaaact cgtccgtcag
     1681 ccgacgaaca agctgcgccg tgggaagaat gtcatgtacc gcccccgcgc cctgtcccgc
     1741 cgaccagacc gtcttccagg cccgcgcctc gtcgcccatg tcgagcttgt gctcgggcag
     1801 ggatttcggg tcgatgccgt tggcgatcag ggacggcgtg aggaagttcg ccggaatgcc
     1861 cgagaccgcg ggggtgtagg tgatgtcgga tgcgcccgcg tcgacgatca tctgcttgta
     1921 ctcgggttgc gccccggatt cggtcgtcga gatgaagcgg gtgcccatat aggcgaagtc
     1981 ggcgcccatc atcagggccg ccgcgacgtc cgaccccgtg gacaggcagc cggacaggat
     2041 gatggtcccg tcgaagaagg atcggacctc gtccacgaga gcgaacggat tgaccacgcc
     2101 cgcgtggccg ccggcgccgt tggccaccag gatcaggccg tcgacgcccg cctgcgccgc
     2161 cttgcgggcg tggcggacat tggcgatgtc gtggaagacc acgccgccgt agccgtgaac
     2221 ggcctcgacc acgtcgcgca cggccccgag ggaggtgatg accagcggcg ccttctcctc
     2281 gaccgtcacc atcatgtcgg ccatcagccg cggattggtc gggtggacga tgtggttgac
     2341 gccccagtgc gccgcgtcgg gcgtcagccg agccttgatc tcgtggatcc agtcccgata
     2401 gccctcggtg gtccgctggt tcagcgacgg gaaggtgcct atgacgccgg cgttgcaggc
     2461 ctcgaccacc agatccggtc ccgagacgag gaacatcggc gcggcgatga ccggcagttt
     2521 caggcctttg cgcagtgagt ccggaatggc catggcggtt ccccgagttg tctttcgcta
     2581 cggatagcgg ggtgtcgccg gggaaggcaa atccatgacg cggcctcggc gggtttgtcc
     2641 cccgccgttt tgtatccctc gcggggacag gtcggggaca aacggagacg aaggcgggac
     2701 agcgggttca gcacaagtgc atatgggcgg atgacgctgg gcctccgaac cgctaaatcg
     2761 cctccatgac caccgacacc tccggcctgc accagctggg ccagcacacc gttcagccgg
     2821 ccagcccgga aaccgccgaa ctggagcgcg tgcccaatcc ccacgccggg accctgtatc
     2881 tgacgcggtt cacggcgccg gagttcacca gcctgtgccc ggtgacgggc cagccggact
     2941 tcgctcacat cgtcatcgac tatgcgccgg gcgactggct ggtcgagagc aagagcctga
     3001 agctgtacct gaccagtttc cgcaatcacg gcgccttcca tgaggactgc accgtggcca
     3061 tcggcaagaa gatcgccgac ctgctgcagc cgaaatggct gaggatcggc ggctactggt
     3121 atccgcgcgg cggcatcccg atcgacgtct tctggcagac gggaccggcg ccggaggggc
     3181 tgtggctgcc ggaccagggc gtgccgacct accgcgggcg ggggtagctc aacgcggcgg
     3241 tctggcgaga tcagcaagct ctatcgcgac ttcgatgtcg tcctcacccc tctatggcat
     3301 atagtatcgg cagcggattg ctgaaggacg ttcgagagat ggtctccaag ctccgccgcg
     3361 gtgttttgat cattttatgg ttttggttcg cttcggtcct cgcgaccgcc cagacgatat
     3421 cgccccctca gaccattgag ccggctgcca gtaacgaaca ggttcttagg gaagattcgg
     3481 gtttctcgcc ggccacaaaa cgtacagcga cgcgcggggc ggcagccttt cgcgcccctt
     3541 cgctagaaac cgtgatccaa ggtcttggcg tcttcgtagc tgccgctgcc gcggtctttt
     3601 cggctatcag cgctcgtggt tccgtggccg cagcaaatga gatgcgccaa gctaggagag
     3661 acgccacaaa accgcgcctg agctaccgcg ttctcgatcg
//
LOCUS       DASVVN010000320         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_433233,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000320 DASVVN010000000
VERSION     DASVVN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433233"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VF686_05140"
     CDS             <1..388
                     /locus_tag="VF686_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8661231.1"
                     /translation="AALWSGLMILLLVVLGARVAMARRGHKVVLGDGGNASVMLASRI
                     FGNASEYIPAGIAALAVLVLLGMPAYVLHGLGGALFLGRLIHAVTLSDQRPTMGRIIG
                     MGLTWLTLILAGLMLIVHAFVGSPHG"
     gene            465..>1580
                     /locus_tag="VF686_05145"
     CDS             465..>1580
                     /locus_tag="VF686_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8661232.1"
                     /translation="MPDRTASASPDILPDLVAAALRAGADAAEAVSAERAALSIGVRN
                     GALEDVEREESRDLGLRVFIGTRQATVSASDLSDATRARLVERAVAMARLAPEDPYAG
                     FAPQDRLARGPFPDLDLYDPADRSAGELEAVAAEAEAAALAVPGVAKSEGGHASASSS
                     RWRLVTSHGFDGAYHGSAFSLGVGVIAEKDGAMERGGENRAVRHLSDLPAADLIGLEA
                     GRRAVARTGPRKIASTTAPVIFENRVAGQVLSPLLGAISGPSVARGTSFLKDRLGQRV
                     LPAGVDLIDDPFRPRGLGSTPFDDEGVAVSRQSIIDDGVLTTWLLNSAAARQLGMAST
                     GHASRGLAGPPGVSTHNLHLEPGEPNLAGLMGGAKIGL"
BASE COUNT          184 a          614 c          541 g          241 t
ORIGIN      
        1 cgccgccctg tggagcggcc tgatgatcct gctgctggtc gtgctcggcg cccgcgtggc
       61 gatggcgcgg cggggccaca aggtggtgct gggcgacggc ggcaacgcca gtgtgatgct
      121 ggcctcgcgg attttcggca atgcctccga atacattccg gcgggaatcg ccgccctggc
      181 cgtcctcgtc ctgctgggga tgccggcgta cgtgttgcat ggtctgggcg gcgcgctgtt
      241 cctgggccgc ctgatccacg ccgtgaccct gagcgaccag cgcccgacca tgggccggat
      301 catcggcatg ggcctgacct ggctgacgct gatcctcgcc ggcctgatgc tgatcgttca
      361 tgccttcgtc ggttcgccgc acggctaggc ggtggtcggc ggtcgcgttc ggcgcgcgtc
      421 gctgagccgg ttgcgcgacg cggccgctcc ggccatatcg gggcatgcct gaccgtaccg
      481 cgtccgcctc ccccgacatc ctgccagatc tggtcgccgc cgccctgagg gccggcgccg
      541 acgccgccga ggccgtctcg gccgagcgcg ccgccctgtc catcggcgtc cgcaatggcg
      601 ccctggagga tgtcgagcgc gaggagagcc gcgacctcgg tctgcgcgtc ttcatcggca
      661 cgcgacaggc gaccgtctcg gcctccgacc tgtccgacgc cacccgcgcc cggctggtcg
      721 agcgcgccgt ggccatggcc cgcctggcgc ctgaagaccc gtacgccggc ttcgccccgc
      781 aggaccggct ggcccgcggc cccttcccgg atctggacct ctacgacccc gccgaccgca
      841 gcgccggcga actcgaggcc gtcgccgcgg aagcggaggc cgccgccctc gccgttcctg
      901 gcgtcgcgaa atccgaaggc ggccacgcct cggcctcgtc cagccgctgg cggctggtga
      961 cctcgcacgg cttcgacggc gcctatcatg gctcggcctt ctcgctcggc gtcggcgtca
     1021 tcgccgagaa ggacggggcc atggagcgcg gcggcgagaa ccgcgccgtc cgccacctgt
     1081 ccgacctccc cgccgccgac ctcatcggcc tcgaggccgg ccgccgcgcc gtcgcccgca
     1141 ccggcccgcg caagatcgcc tcgaccaccg ccccggtgat cttcgagaac cgcgtcgccg
     1201 gacaggtcct gtccccgctg ctgggcgcca tctcgggccc ctcggtggcg cgcggcacct
     1261 cctttctcaa ggaccggttg ggccagcgag tcctgcccgc cggcgtcgac ctgatcgacg
     1321 accccttccg cccccgcggc ctcggctcga ccccgtttga cgatgaaggc gtggccgtaa
     1381 gccgccaatc aatcattgat gatggtgttc tgacgacgtg gctgctgaac agcgccgccg
     1441 cgaggcagct ggggatggcc tcgaccggcc acgcctcgcg cggcctcgcc ggcccgcccg
     1501 gggtctcgac ccacaacctg catctggagc cgggcgagcc caatctggcc ggcctgatgg
     1561 gcggcgcgaa gatcggccta
//
LOCUS       DASVVN010000321         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_143748,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000321 DASVVN010000000
VERSION     DASVVN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143748"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1509)
                     /locus_tag="VF686_05150"
     CDS             complement(61..1509)
                     /locus_tag="VF686_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8661233.1"
                     /translation="MLAGSLALTACATTGGDDTPAADGSSDAGAREPLARGLDYAANP
                     DPFPSTYRGLPRENMAIVGGTVFDGAGRKIENGVVIVTDGKVAAVGDASTAVPGGHRV
                     VDARGRFVTPGIIDVHSHLGVYPSPGVQGMSDGNEATNPNTAQVWAEHSLWPQDPGFN
                     AARAGGVTTLQILPGSANLFGGRGVTVRNVPSVTMQGMKMPGAPYGLKMACGENPSRV
                     YGGRNTSPATGMGNVAGYRAAFIAAREYKAKWDKWREDGEGSPPTRNLQLETLAGVLD
                     GSVLIQNHCYRADEMAVMIDIAKEFGYRITAFHHAIEAYKVAPLLAREGICADMWTGW
                     WGFKMEALDGVEANAALVDAPQGSCAVIHSDDAELTQRLNQEAAAGLAAGRRIGMNIS
                     EERAMSWITLNPARSLGIADQTGSLEAGKRADVVIWSANPFSVYARADQVFIDGGLSF
                     DRMNPAYQPLSDYELGQPGFGLSVANVPQGAR"
BASE COUNT          216 a          520 c          541 g          234 t
ORIGIN      
        1 aggccggcgt ggtcagggct gcggcgcaga cggcgccgag gatgaggttc ctgaggcgca
       61 tcagcgggct ccctgcggga cgttggcgac ggagagaccg aagccgggct ggcccagctc
      121 atagtcggac agcggctgat aggccgggtt catccggtcg aagctcaggc cgccgtcgat
      181 gaagacctga tcggcgcggg cgtagacgct gaacggattg gcgctccaga tcaccacatc
      241 ggcgcgcttg ccggcttcca gcgatccggt ctggtcggcg atgcccagcg agcgggccgg
      301 gttcagggtg atccagctca tcgcccgctc ctcggagata ttcatcccga tgcggcgccc
      361 cgcggccagg ccggcggcgg cttcctgatt gaggcgctgg gtcagttcgg cgtcgtccga
      421 atggatgacc gcgcacgatc cctgcggcgc atcgaccagc gccgcattgg cctcgacccc
      481 gtccagagcc tccatcttga agccccacca gccggtccac atgtcggcgc agatgccctc
      541 gcgggccagc agtggcgcga ccttgtaggc ctcgatggcg tggtggaagg cggtgatccg
      601 gtagccgaac tccttggcga tatcgatcat caccgccatc tcgtcggcgc ggtagcagtg
      661 gttctggatc agcaccgacc catccagcac cccggccagg gtctcgagct gcaggttgcg
      721 ggtcgggggc gagccctcgc cgtcctcgcg ccacttgtcc catttggcct tgtattcgcg
      781 ggctgcgatg aaggcggcgc gatagccggc gacattgccc atgccggtgg ccggcgaggt
      841 gttgcgcccg ccatagacgc gcgacgggtt ctcgccgcag gccatcttca ggccataggg
      901 ggcgccgggc atcttcatgc cctgcatggt caccgacggc acattgcgca ccgtcacgcc
      961 gcggccgccg aacagattgg ccgagccggg caggatctgg agcgtggtca ccccgccggc
     1021 gcgcgccgca ttgaagccgg ggtcctgggg ccacagcgaa tgctcggccc agacctgggc
     1081 ggtgttcgga ttggtcgcct cgttgccgtc gctcatcccc tgcacgcccg gcgagggata
     1141 gacgccgagg tgcgagtgca cgtcgatgat gcccggggtc acgaaccggc cgcgcgcatc
     1201 cacgactcgg tggccgccgg gaaccgccgt cgaggcgtcg ccgaccgcgg ccaccttgcc
     1261 gtcggtgacg atgaccacgc cgttctcgat cttgcggccg gccccgtcga agaccgtgcc
     1321 gccgacgatg gccatgttct cgcgcggcag gccgcgatag gtcgaaggga aggggtcggg
     1381 gttggccgca tagtccaggc cccgggccag cggctcgcgc gccccggcgt ctgacgaacc
     1441 gtccgcggcg ggcgtgtcgt cgcccccggt ggtggcgcag gccgtcaggg ccagagagcc
     1501 cgccagcacg c
//
LOCUS       DASVVN010000322         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_433419,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000322 DASVVN010000000
VERSION     DASVVN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433419"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VF686_05155"
     CDS             complement(<1..528)
                     /locus_tag="VF686_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8661234.1"
                     /translation="MTPAARLASAASVLDSIAQGRAPADVVLKAWGAANRYAGSKDRR
                     AVADRVYEVLRARGRLVRAMGGREDGRALVIGSLAFIDGLSLEEIEALHSGDGYGPRP
                     LSKQERARIEAGEADLPDWAAAGLPEFVAEDLKTTFGDRWNEEAAALMARAPVDLRVN
                     TAKTTVDAARRDLIAG"
     gene            complement(538..936)
                     /locus_tag="VF686_05160"
     CDS             complement(538..936)
                     /locus_tag="VF686_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8661235.1"
                     /translation="MDMTPEFIMLAFVLILAIVQILAAGAARTAELGGKWNAGPRDGD
                     VPPPGKMAGRLMRAQANLFETLPIFAAAVIMAHIAGKDGQLTFIGTHLYFFGRLIYLP
                     LYAFGVPYIRSLVWLVATAGLVMVIAALFV"
     gene            complement(957..2417)
                     /gene="guaB"
                     /locus_tag="VF686_05165"
     CDS             complement(957..2417)
                     /gene="guaB"
                     /locus_tag="VF686_05165"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX8661236.1"
                     /translation="MEIREGLTFDDVLLEPGPSEIMPADADVSTQLTRDIRLNIPLTS
                     SAMDTVTESRLAIAMAQAGGLGILHRNMTVEEQADQVREVKRYESGMVINPVTITPQT
                     TLGEIRAIVAKKKISGFPVVDPVSGKLVGILTNRDMRFDTDPTRKAIELMTTGDLVTV
                     REGAGRDEARALLRDRKIERVIVVDEDYRATGLITMKDIEKAQAHPHAAKDDQGRLLV
                     GAASTVGDAGYERAMALAEAGVDVVVIDTAHGHSAQVSRVVERIKRESNRIQIIAGNV
                     ATHDAARALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIMEAVRAAQASGAPVI
                     ADGGIKYSGDLAKAIAAGASCAMMGSMFAGTDESPGEVFLYQGRSYKSYRGMGSVGAM
                     GAGSADRYFQKEVEDTQKLVPEGIEGQTPYKGPIAPVLHQMVGGLRAAMGYVGAATIA
                     DLQERARFVRITGAGLRESHVHDVMITREAPNYRQG"
     gene            2549..3097
                     /locus_tag="VF686_05170"
     CDS             2549..3097
                     /locus_tag="VF686_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEX8661237.1"
                     /translation="MVRTLISTARRLALKIASYGVMHLVVAILVAFAITRDWRMALAV
                     GVVEPFFQTIAYSIHDRVWHRIEQRKRLSGLEEASEAFTARLQIMAPEEQTRAHGHHG
                     HSHALPRSFKQIALKSVTYGVMHFVVAVTVAFALTRDWRVALAIGIIEPLVQTVFFTV
                     HDRVWTRIEARKAAKAAEMASA"
     gene            complement(3102..3824)
                     /locus_tag="VF686_05175"
     CDS             complement(3102..3824)
                     /locus_tag="VF686_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX8661238.1"
                     /translation="MSDEEKPAAEEPARRRMVRPPSGGTAAGRGMGTKMKTADTKSMS
                     SQQWIKRQLSDKWSERARAEGWRSRAAFKLLEIDDRFHLIKHGSRVIDLGAAPGGWAQ
                     VAVKRGATSVVGVDLLPVEPVEGAIMLQADFTHPGVDQQMIDLLGGPPDLVLSDMAHN
                     TVGHRQTDHLKIIALIEIAADFALRTLRPGGHFVSKNFQGGDAGGVLARLRAGFETVK
                     YVKPASSRKDSAEVFLVALNKR"
     gene            3893..4360
                     /locus_tag="VF686_05180"
     CDS             3893..4360
                     /locus_tag="VF686_05180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8661239.1"
                     /translation="MADETLTFTLDTGDGVDRDVVIKLRPDLAPGHVARITELANEGF
                     YDGVVFHRVIPGFMAQGGDPTGTGTSGSKKPNLKAEFSKERHARGVCSMARTSDPNSA
                     NSQFFICLDDASFLDGQYTVWGEVTEGMEHVDALPKGEPPRNPGKIVKASLRA"
BASE COUNT          683 a         1531 c         1433 g          715 t
ORIGIN      
        1 accggcgatc aggtcgcgac gggccgcatc gaccgtcgtc ttcgccgtgt tgacgcgtag
       61 atcgacgggg gcgcgggcca tcagggcggc ggcctcctcg ttccagcgat cgccgaaggt
      121 ggtcttcagg tcctcggcca cgaactcggg caggccggcg gcggcccagt cgggcagatc
      181 ggcctccccg gcctcgatgc gggcgcgctc ctgtttcgac agcgggcgcg ggccgtagcc
      241 gtcgcccgag tgcagggcct cgatctcttc cagcgacaag ccgtcgatga aggccagcga
      301 gccgatcacc agcgcccgcc cgtcctcgcg cccgcccatg gcgcggacca gacggccgcg
      361 cgcgcgcagc acctcataga cccgatcggc gacggcgcgg cggtccttgg accccgcata
      421 gcggttggcg gcgccccagg ccttgaggac cacatcggcg ggcgcgcggc cctgggcgat
      481 gctgtcgagg acggaggcgg cggaggccag acgagctgcg ggggtcaaat caaactttca
      541 gacaaaaagg gcggcgatca ccatcaccag tccggcggtg gcgaccagcc agaccagcga
      601 gcggatgtag ggaacgccga aggcgtacag cggcagatag atcagccgcc cgaagaaata
      661 gagatgcgta ccgatgaagg tgagctggcc gtccttaccg gcgatatgcg ccatgatcac
      721 ggcggccgcg aaaatcggca gggtctcgaa cagattggcc tgcgcccgca tcagccggcc
      781 ggccatcttg cctggaggcg gaacgtcgcc gtcgcggggt ccggcgttcc atttgcctcc
      841 cagctcagcg gtccgggccg cacccgccgc caagatctgg acgatggcca ggatcagcac
      901 gaaggccagc atgatgaact cgggcgtcat atccatggcg tcagtcccct ctccggtcag
      961 ccctgcctgt agttcggcgc ttcccgcgtg atcatcacgt catggacgtg gctctcgcgc
     1021 agaccggcgc cggtgatgcg gacgaagcgg gcccgctcct gcagatcggc gatggtggcg
     1081 gcgccgacat agcccatggc ggcccgcagc cccccgacca tctggtgcag gaccggggcg
     1141 atcggcccct tgtacggcgt ttggccttcg atgccctcgg gcaccagttt ctgggtgtcc
     1201 tccacctcct tctggaagta gcggtcggcc gatccggcgc ccatggcccc gaccgagccc
     1261 atgccgcggt acgacttgta ggaccggccc tggtacagga agacctcgcc ggggctttcg
     1321 tcggtgcccg cgaacatcga gcccatcatg gcgcaggacg ccccggccgc gatggccttg
     1381 gccagatcgc ccgaatattt gatgccgccg tcggcgatga cgggggcgcc ggatgcctgg
     1441 gcggcgcgga cggcctccat gatggcggtc agctggggga cgcccacgcc cgcgacgatg
     1501 cgggtggtgc agatgctgcc cgggccgatc ccgaccttca cggcgtcggc gccggcgtcg
     1561 atcagggctc gcgcggcgtc atgggtggcg acgttgccgg cgatgatctg gatgcggttc
     1621 gattcccgct tgatgcgctc gaccacgcgc gagacctggg ccgagtggcc gtgggcggtg
     1681 tcgatcacca ccacgtccac gccagcctcc gccagcgcca tggcccgctc atagcccgcg
     1741 tcgccgacgg tcgaggcggc cccgaccagc agccggccct gatcgtcctt ggcggcgtgc
     1801 ggatgggcct gggccttctc gatgtccttc atcgtgatca ggccggtggc gcgatagtcc
     1861 tcgtcgacca cgatgacgcg ctcgatcttg cggtcccgca gcagggcccg cgcctcgtcg
     1921 cgtccggcgc cctcgcgcac ggtgaccaga tcgcccgtgg tcatcagctc gatggccttg
     1981 cgggtcggat cggtgtcgaa acgcatgtcc cgattagtaa ggatgccgac cagcttgccg
     2041 ctgaccggat cgacgaccgg gaagcccgag attttcttct tggccacgat ggcgcggatt
     2101 tcgcccagcg tcgtctgcgg cgtgatggtc accggattga tgaccatgcc gctttcgtag
     2161 cgtttgacct cgcggacctg atcggcctgt tcctcgaccg tcatattgcg gtgaagaatg
     2221 cccagaccgc cggcctgagc catggcgatc gccagccggc tttcggtgac cgtgtccatc
     2281 gcggacgagg tcagcgggat gttcagcctg atgtcgcggg tcagctgcgt cgagacatcc
     2341 gcgtctgcgg gcatgatctc tgacgggccg ggttccagca aaacatcgtc gaaggtgagc
     2401 ccttcgcgta tctccatgag gagtctccgt tttgcgggcc gcgtatagcg gtgaccggcg
     2461 gggaaccgca agggccgctc gcccttatcg acgcgataag aacaattggc ttgccggatg
     2521 cggcgccggt cccacattgg ggagacatat ggtccgcacg ctcatcagca ccgcccgccg
     2581 cctcgcgctc aagatcgcga gctatggcgt gatgcacctg gtggtcgcca tcctggtggc
     2641 cttcgccatc acccgcgact ggcggatggc tctggccgtc ggcgtcgtcg agcccttctt
     2701 ccagaccatc gcctattcga tccacgaccg cgtctggcac cggatcgagc agcgcaagag
     2761 gctctccggc ctcgaggagg cgtccgaggc cttcaccgcc cgcctgcaga tcatggcgcc
     2821 ggaggaacag acccgcgccc acggccacca cggccacagc cacgccctgc cccgctcgtt
     2881 caaacagatc gcgctgaagt cggtgaccta tggcgtcatg catttcgtcg tcgcggtgac
     2941 ggtggccttc gccctgaccc gcgactggcg ggtggccctg gccatcggca tcatcgagcc
     3001 gctggtccag acggtcttct tcaccgtgca cgaccgcgtc tggacccgga tcgaggcgcg
     3061 caaggcggcc aaggcggcgg aaatggcctc ggcctgaccg cctaccgctt gttcagcgcc
     3121 accagaaaca cctcggcgct gtccttccgg ctcgaggccg gcttgacgta tttcaccgtc
     3181 tcgaaccccg cacgcagccg cgccagaacg cccccggcgt cgccgccctg gaaattcttg
     3241 gacacgaaat gcccgcccgg ccgcaaggtg cgcagggcga agtcggcggc gatctcgatc
     3301 agggcgatga tcttcaggtg gtcggtctgg cggtggccga cggtgttatg ggccatgtcg
     3361 gacaggacca gatcgggcgg accgcccagc agatcgatca tctgctggtc cacgcccgga
     3421 tgggtgaaat cggcctggag catgatagcg ccctccaccg gctcgaccgg cagcagatcc
     3481 accccgacca ccgatgtcgc gccgcgcttg accgcgacct gggcccagcc gcccggcgcg
     3541 gcccccaggt cgatgacccg gctgccgtgc ttgatcagat gaaaccggtc gtcgatctcc
     3601 agcagtttga acgccgcccg gctgcgccag ccctcggccc ttgcccgctc ggaccatttg
     3661 tccgagagct ggcgcttgat ccactgctgg ctggacatcg acttggtgtc cgccgtcttc
     3721 atcttggtgc ccatgccgcg gccggcggcg gtcccgcccg agggcgggcg caccatccgg
     3781 cggcgtgcag gttcttcggc tgcgggcttt tcttcgtcac tcatcgcatc cacatagccc
     3841 ccgacgggtt tgcgggctat gcacccgcga taattcacaa ggagcgcgcc ccatggccga
     3901 cgaaaccctg accttcaccc tggacaccgg cgacggcgtc gaccgcgatg tcgtcatcaa
     3961 gctgcgcccc gacctggccc ccggccacgt cgcccgcatc accgagttgg ccaacgaagg
     4021 cttctacgac ggcgtggtct tccaccgcgt cattcccggc ttcatggccc agggcggcga
     4081 tccgaccggc accggcacct cgggctcgaa gaagcccaac ctgaaggccg agttctccaa
     4141 agagcgccac gcccgcggcg tctgctccat ggcccgcacc tcggatccca acagcgccaa
     4201 cagccagttc ttcatctgcc tggacgacgc ctccttcctc gacggtcagt ataccgtgtg
     4261 gggcgaagtg accgaaggca tggaacatgt cgacgcgctg cccaagggtg agccgccgcg
     4321 gaacccgggc aagatcgtca aggcgagcct gcgcgcctga ac
//
LOCUS       DASVVN010000323         6164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_382047,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000323 DASVVN010000000
VERSION     DASVVN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382047"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /gene="nusA"
                     /locus_tag="VF686_05185"
     CDS             complement(<1..1700)
                     /gene="nusA"
                     /locus_tag="VF686_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8661240.1"
                     /translation="MAVAGVSANRLELLQIADAVAREKNIDKEIVVEAIEEAIQKGAK
                     SRYGAHHDIRAKIDIKSGELALTRHVTIVADDWQPEDELEEFNDSAMVRLTDASKRDP
                     EAFVGKEYVETLPPFEFGRVQTQMARQVVTGKVREAERANQYEEFKDRVGEIVNGTVK
                     RVEYGNTVVDLGRGEGIMRRNDSIPREVFNIGDRIRTYIYDVRAEAKGPQVMLSRSHP
                     GFMAKLFAQEVPEVYDGVIEIRAAARDAGSRAKMAVLSNDSSIDPVGACVGMRGSRVQ
                     AVVAELQGEKIDIIQWNNDEPTFIVNALAPAEVSKVVLDEEADRVEVVVPDEQLSLAI
                     GRRGQNVRLASQLTGWQIDIITESQDSERRQREFSERTTLFQEALDVDEVIAQLLVTE
                     GFATVEDLAFVEPYEISDIEGFDEDTAEELQARARDFLDRQAAILDAKRVELGVEDGV
                     MAVPGVTGAIAVALGEGGVKTVEDLADLATDEIRGGYEVKNGERVKVPGVLESFNLAQ
                     EEAEMLILQARVAAGWIDASELPQPPEPEYEEELPEGEYDAEAVFAAAPEGDEAEAVD
                     "
     gene            complement(1703..2245)
                     /gene="rimP"
                     /locus_tag="VF686_05190"
     CDS             complement(1703..2245)
                     /gene="rimP"
                     /locus_tag="VF686_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX8661241.1"
                     /translation="MKAKTAEDRQLLELIEPVAESLGLDIVRVRLMGGTLRRRLQIMA
                     ERPSDHDIAVEECARLSRAVSEVFDAADPINGEYLLEVSSPGVDRPLTRQSDFDLFEG
                     YEARLETDRMIEGRKRFKGVIAGTDGENIAMDLEGEEDTALIPFEWLVDAKLVLTDEL
                     MKAGAEKRAARNDNSTETED"
     gene            complement(2383..2826)
                     /locus_tag="VF686_05195"
     CDS             complement(2383..2826)
                     /locus_tag="VF686_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661242.1"
                     /translation="MRFAFGTLSAGAVISTAALLGACAADPVMYRLEATNLAFAGLAG
                     GTTATAVRTSSGWSVTRQGFYRGSRPKRVRLTQAEALALDTALSDPALYVSPQPLPGG
                     CIDPDLTTLDITANGATRRLSQECATAPGMSSVLRILFGWPRGGD"
     gene            complement(2890..3393)
                     /locus_tag="VF686_05200"
     CDS             complement(2890..3393)
                     /locus_tag="VF686_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX8661243.1"
                     /translation="MSLTLKDASAPIFLRGLRVLSGLIEKAEASGLGEAAIMDARLAP
                     DMKPFPDQIRMASFSARSCVARLTGQAWPKTDDSEATLADLKATIDLSIAFIEATDPA
                     AFEGGETRRIEIRFPGVELDFEGAGYLTSFAIPNFYFHVSMAYAILRNAGVELGKRDY
                     LGQLALI"
     gene            3624..4079
                     /locus_tag="VF686_05205"
     CDS             3624..4079
                     /locus_tag="VF686_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX8661244.1"
                     /translation="MPMTFQTWAVLLAGAATGPVRGMSAVALYLVAGLAGLPVLADGA
                     SGPEPFTGPTAGYLAAFLAAAGLAGWLSVTGRLARPLSAIVLMIGLHLLILAVGSAWL
                     ALSIGAAPALEHGVLPFLPGAVIKSILVVAAAFGLVRVARFTPARFRRA"
     gene            complement(4066..4761)
                     /gene="trmB"
                     /locus_tag="VF686_05210"
     CDS             complement(4066..4761)
                     /gene="trmB"
                     /locus_tag="VF686_05210"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX8661245.1"
                     /translation="MSSSDDPAHRPLRSFGRIKSRAIKPRQAALFDTLLPAIAVPDPK
                     AGPVDPVALMPDAKGVWLEIGFGGGEHLAEQASRHPEVLMIGCEPFLNGVGSALRHID
                     ERGLKNVRLHADDARDVMTALPDASLARVMILFPDPWHKARHNKRRLVQKETAAEIRR
                     LLKPGGVVRFVTDWADYAEWALEKFQQTPDLVRVGSPGDDWTAPWADHVVTRYEEKRL
                     GDTTPLFMEFRRA"
     gene            4851..5096
                     /locus_tag="VF686_05215"
     CDS             4851..5096
                     /locus_tag="VF686_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661246.1"
                     /translation="MADIIGWSMGLALIGLVVWATVAMAGGGGRKAARRRGGAQMMAA
                     MLLGFGAALDPPQRHVAEAKDETPKSSPETGEPELDD"
     gene            complement(5093..>6164)
                     /gene="metK"
                     /locus_tag="VF686_05220"
     CDS             complement(5093..>6164)
                     /gene="metK"
                     /locus_tag="VF686_05220"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX8661247.1"
                     /translation="LTADPYSRVGCETLVTTNRIVLAGEVRGPTGVLESLEDKVRAAV
                     KDIGYDQEGFSWHKSVFENHLHGQSADIAVGVDAAGNKDEGAGDQGIMFGYASNETPE
                     LMPATLQYSHNILKRLAEVRHAGDTRLEPDAKSQVTIQYEDGKPVRATSIVLSTQHAA
                     GLSSADVAAIVKPYILEVLPEGFTDENTVWHINPTGIFEIGGPDGDAGVTGRKIIVDT
                     YGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLAERCTIQISYAIGVAKPQ
                     SIHVDLHGTGRGVVTEAVLEANILGLIGGATPRAIREHLGLNKPIYARTAAYGHFGRA
                     PDADGGFSWEKTDLVDQLKALA"
BASE COUNT          899 a         2103 c         2064 g         1098 t
ORIGIN      
        1 tcatcgacgg cttcggcctc gtcgccttcc ggcgcggcgg cgaagacggc ttcggcgtca
       61 tattcgccct cgggcagttc ttcctcgtat tccggctcgg gcggctgcgg cagttcggag
      121 gcgtcgatcc agccggcggc gacccgcgcc tgcaggatca gcatttcggc ctcttcctgg
      181 gcgaggttga agctttccag cacgcccggg accttgaccc gttcgccgtt cttcacttcg
      241 tagccgccgc ggatttcgtc ggtggccaga tcggccagat cctcgacggt cttcaccccg
      301 ccttcgccca gggcgacggc gatggcgccg gtcacgcccg gaacggccat gacgccgtcc
      361 tcgacgccca gctcgacgcg tttcgcatcg aggatggccg cctgacgatc caggaagtcg
      421 cgggcgcgag cctgcagttc ctcggccgtg tcctcgtcga agccctcgat gtccgagatt
      481 tcgtacggct cgacgaaggc caggtcctcg accgtggcga agccttcggt gaccagcagc
      541 tgggcgatga cctcgtccac gtccagggct tcctggaaca gggtggtgcg ttcgctgaac
      601 tcgcgctggc ggcgctcgga gtcctggctc tcggtgatga tgtcgatctg ccagccggtc
      661 agctgcgagg cgaggcggac gttctggccg cggcggccga tggccagcga cagctgctcg
      721 tccggcacca ccacctcgac gcggtcggct tcctcgtcga ggacgacctt ggacacctcc
      781 gccggggcca gggcgttgac gatgaacgtc ggctcgtcgt tgttccactg gatgatgtcg
      841 atcttctcgc cctgcagttc ggcgacgacc gcctgaaccc gcgagccgcg catgccgacg
      901 caggcgccga ccggatcgat ggaggaatcg ttcgacagca cggccatctt ggcgcgcgaa
      961 ccggcgtcgc gggcggcggc gcggatctcg atcacgccgt cgtagacttc gggcacttcc
     1021 tgggcgaaca gcttggccat gaagccgggg tgcgagcgcg acagcatcac ctgcgggccc
     1081 ttggcctcgg cgcggacgtc gtagatgtag gtgcggatcc ggtcgccgat gttgaacacc
     1141 tcgcgcggga tcgagtcgtt gcggcgcatg atgccctcgc cccggcccag atcgacgacg
     1201 gtgttgccgt attcgacgcg cttgacggtg ccgttgacga tctcgccgac gcgatccttg
     1261 aactcttcgt actggttggc gcgctcggcc tcgcggacct tcccggtcac gacctgacgg
     1321 gccatctgcg tctggacccg gccgaactcg aacggcggca gggtctcgac gtattccttg
     1381 ccgacgaagg cttccggatc gcgcttggag gcgtcggtca agcggaccat ggccgagtcg
     1441 ttgaactctt ccagctcgtc ttccggctgc cagtcgtccg cgacgatggt gacgtgacgc
     1501 gtcagggcca gctcgcccga cttgatgtcg atcttggcgc ggatgtcgtg atgggcgccg
     1561 taacgcgatt tggcgccctt ctggatcgcc tcctcgatcg cctcgacgac gatctccttg
     1621 tcgatgttct tctcgcgcgc gaccgcgtca gcgatctgaa gcagttcgag tcggttcgcg
     1681 gaaacgccgg caacagccat gatcagtcct cggtctcggt agagttgtcg ttacgggcgg
     1741 cgcgcttctc tgcgcccgcc ttcatcagtt cgtcggtcag caccagtttg gcgtcgacca
     1801 gccattcgaa gggaatgagg gcggtgtcct cctccccttc cagatccatg gcgatgttct
     1861 cgccgtccgt gccggcgatc acgcccttga agcgtttgcg gccctcgatc atgcgatcgg
     1921 tttccagtcg cgcctcatag ccctcgaaca gatcgaagtc cgactgacgg gtcagggggc
     1981 ggtccacgcc cggcgacgac acttccagca gatactcgcc gttgatcgga tcggcggcgt
     2041 cgaacacttc cgacaccgcg cggctcagac gggcgcattc ctcgaccgcg atgtcgtggt
     2101 cgctcggccg ctcggccatg atctgcagcc gccggcgcaa ggtgccgccc atcagccgca
     2161 cgcgcacgat gtcgagaccc aggctctcgg ccacgggttc gatcagctcc agcagctggc
     2221 ggtcttcggc ggtcttggcc ttcaaaggct ttggtccaaa caaaaagcgg cgggcccgac
     2281 ggccgccgct tcaaacggcg ccgttaggac gccggtctaa cgatgtggga gggcgtatag
     2341 ctcccccgac ggggtttgtc agcccccgtt cgcggcgcag cttcaatcgc cgccccgtgg
     2401 ccaaccgaac agtatccgca ggacggacga cataccgggg gccgtcgcgc actcctggct
     2461 caatcgccgt gtggctccgt tcgcggtgat gtccagcgtc gtcaggtcgg gatcgatgca
     2521 ccctccgggc aaaggctgcg gcgaaacgta caaggcggga tcagacagcg ctgtatccag
     2581 cgccagcgcc tcggcttgcg tgagtcgaac ccgcttcggc cttgagccgc gataaaaccc
     2641 ctgcctcgtc acggaccagc ccgatgaggt tcggactgcg gtggccgtcg tgccgcccgc
     2701 caaacccgcg aaggccagat tggtggcttc aaggcgatac atcaccgggt cagcggcgca
     2761 cgcgcccagc agcgccgccg tgctgatgac cgcccccgcg ctcaacgtcc cgaaggcaaa
     2821 cctcacgacg aatctccgac tcgaaacagg ctccggaaag aactgcggcc tgccagctcg
     2881 ttcccgccct cagatcagcg ccagctggcc cagataatcg cgcttgccca gctcgacccc
     2941 ggcgttgcgc agtatcgcgt aggccatgct gacgtggaaa tagaagttcg gaatggcgaa
     3001 gctggtcaga tagccggcgc cttcgaaatc gagttcgacg cccgggaagc ggatttcgat
     3061 ccggcgcgtc tcgccgccct cgaacgccgc cgggtccgtc gcctcgatga aggcgatcga
     3121 caggtcgatg gtcgccttca ggtcggcgag cgtcgcttcg gaatcgtccg tcttcggcca
     3181 cgcctgaccc gtcagccgcg ccacgcaact gcgcgccgaa aacgaagcca tgcggatctg
     3241 gtcgggaaac ggcttcatgt ccggggccag ccgggcgtcc atgatcgccg cctcgccgag
     3301 accggaggcc tcggctttct cgatcaggcc ggacaatacg cgcagcccgc gcaggaagat
     3361 cggcgcggag gcgtctttca gggtcaggct catggcgatt ctccagttct cgttgctccg
     3421 ctcgtgcact ggttccgccc gggaatccag cggccgctcc cgacttgcat gccgcgggac
     3481 cacatggtct tgcacaggcc atgcctctcg ccccgccgcc acatccaata cagccgccgg
     3541 ccgccctcgt ctggcttgtg tctgtggcgg gcttcgcgct cctgatggcc gtgagcgccc
     3601 gcatcgctgt tcccatgatt ccggtgccga tgacctttca gacctgggcc gtgctgctgg
     3661 cgggcgcggc gacgggaccc gtccggggga tgtccgccgt ggcgctgtat ctcgtcgcag
     3721 gcctcgccgg tctgcccgtg ctggccgacg gcgcgtcagg cccggagccc ttcaccggac
     3781 cgaccgccgg ctatctggcc gccttcctgg ccgccgcggg tctggcgggc tggctgtccg
     3841 tgaccggtcg tctggcgcgg cctctttccg ctatcgtctt gatgatcggc ctgcacctgc
     3901 tgatcctcgc cgtgggcagc gcatggctgg ccctgagcat cggcgccgca ccggcgctgg
     3961 agcacggcgt actgcccttc ctgccagggg cggtcatcaa gtccatcctg gtcgtcgcgg
     4021 ccgcctttgg gctcgtccgc gtggcgcggt tcacgccggc gaggttcagg cgcgcctgaa
     4081 ctccatgaac agcggcgtcg tgtcgcccag ccgcttttcc tcatagcggg tcacgacatg
     4141 gtccgcccat ggcgcggtcc agtcgtcgcc gggcgagccg acgcgcacga ggtccggggt
     4201 ctgctgaaac ttctccagcg cccattcggc atagtccgcc cagtctgtga cgaaccggac
     4261 gacgccgccg ggtttgagca gccgcctgat ctccgccgcc gtctctttct ggaccagccg
     4321 ccgtttgttg tgccgcgcct tgtgccaggg atcggggaac aggatcatca cccgagccag
     4381 actggcgtcc ggcagggccg tcatcacgtc gcgcgcgtca tccgcgtgca ggcggacgtt
     4441 cttcaggccg cgctcgtcga tatgccgcag ggccgacccc accccgttca gaaacggttc
     4501 gcagccgatc atcagcacct caggatgccg gctcgcctgt tcggccagat gctccccgcc
     4561 gccaaaaccg atctccagcc agacgccctt cgcatccggc ataagggcga cgggatcgac
     4621 cgggcccgcc ttcgggtcag gcacggcgat ggccggcagc aaagtgtcga acagcgccgc
     4681 ctgccgcggc ttgatggcgc gcgacttgat gcgaccgaac gagcggagcg ggcggtgagc
     4741 gggatcgtcg gaagaagaca tgggtggcct tataggcggt cgggcgcggg gcgcgagtcc
     4801 ttcacagcgc tcttcgccgc cccatgttca tggagcatcg agggcggacc atggcggata
     4861 tcatcggctg gagcatgggc ctcgccctga tcggcctggt cgtctgggcg accgtcgcca
     4921 tggcgggcgg cggcgggcgg aaggccgcga gacggcgtgg cggcgcgcag atgatggcgg
     4981 ccatgctgtt gggtttcggg gcggccctgg acccgccgca gcgccatgtg gccgaagcga
     5041 aggatgaaac gccgaagagc tcgccggaga ccggcgagcc cgaactcgac gactaggcca
     5101 gcgccttcag ctggtcgacc agatcggtct tctcccagct gaacccgccg tcggcgtccg
     5161 gcgcccggcc gaaatggcca taggcggcgg tgcgggcgta gatcggcttg ttgaggccca
     5221 ggtgctcgcg gatggcgcgc ggggtggcgc cgccgatcag gccaagaatg ttcgcctcca
     5281 gcaccgcctc cgtgacgacg ccccggcccg tgccatgcag gtcgacgtgg atcgactggg
     5341 gcttggccac gccgatggcg tagctgatct ggatggtgca gcgttcggcc aggccggcgg
     5401 cgacgacgtt cttggccaga tagcggcagg cataggcggc cgaacgatcg accttggtcg
     5461 gatccttgcc cgagaaggcg ccgccgccgt gcggagccgc gccgccgtag gtgtcgacga
     5521 tgatcttgcg accggtcacg cccgcgtctc cgtccggtcc gccgatctcg aagatgccgg
     5581 tcggattgat gtgccagacg gtgttctcgt cggtgaagcc ttccggcagc acctcgagga
     5641 tgtagggctt cacgatcgcg gcgacgtcgg cggacgacag gccggcggcg tgctgggtcg
     5701 acaggacgat cgaggtggcg cgcaccggct tgccgtcctc gtactggatc gtgacctggc
     5761 tcttggcgtc tggctccagc cgcgtgtcgc cggcgtggcg cacctcggcc agacgcttca
     5821 ggatgttatg gctgtactgc agggtggccg gcatcagctc gggcgtctcg ttcgaggcgt
     5881 aaccgaacat gatgccctga tcgcccgcgc cctcatcctt gttgccggcg gcgtcgacgc
     5941 ccaccgcgat gtcggccgac tggccgtgca ggtggttctc gaagacggat ttgtgccagg
     6001 agaagccttc ctggtcgtag ccgatatcct tcaccgccgc ccggaccttg tcctccaggc
     6061 tctcgagcac gcccgtgggg ccgcggacct cgccggcgag gacgatgcgg ttggtggtga
     6121 ccagggtctc gcagccgacg cgggaatagg gatccgcggt cagg
//
LOCUS       DASVVN010000324         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_484193,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000324 DASVVN010000000
VERSION     DASVVN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484193"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..771)
                     /gene="hisA"
                     /locus_tag="VF686_05225"
     CDS             complement(40..771)
                     /gene="hisA"
                     /locus_tag="VF686_05225"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX8661248.1"
                     /translation="MIVYPAIDLRQGVCVRLMHGKFDEVTRYDDQPAARLAAFAADGA
                     IWAHIVDLDGAEAGRAVQHALIGELAAAIDIRIQSGGGVRSADDVQALLDAGVARVVV
                     GSLAVSQSWAVADWLARFGADRITLAIDVKADGDRWIPALKGWTEAAEVDLWAALDRY
                     PPGLARHLLVTDVGRDGALTGPNIDLLGEIARRRPDLRVQASGGVSQLSDLTAARDAG
                     CDGVIVGRALYENRFTVAEALAATA"
     gene            complement(782..1375)
                     /gene="hisH"
                     /locus_tag="VF686_05230"
     CDS             complement(782..1375)
                     /gene="hisH"
                     /locus_tag="VF686_05230"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX8661249.1"
                     /translation="MTVLAYGAGNTASVLFALERLGATVRLTADPAEVADARRLILPG
                     VGAAAYAMARLRELGLVEPIRAFQRPLLGVCLGQQLLFEASDEGGGTAMLGLIPGRVT
                     ALAPGPGLPVPHMGWSRLSQIKDDPLLERLEEGAYAYFVHGFVCPDGPATLACADYGG
                     PVPAIVRAGNRWGCQFHPERSAAAGACILKNFLELPQ"
     gene            complement(1381..1992)
                     /gene="hisB"
                     /locus_tag="VF686_05235"
     CDS             complement(1381..1992)
                     /gene="hisB"
                     /locus_tag="VF686_05235"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX8661250.1"
                     /translation="MAALGLGAGSGRAHRTGQAVRDTKETRIVCAVDLDATGPVSIAT
                     GVGFFDHMLEQVATHGGFSLRLACEGDLHTDPHHTIEDSAIALGQALKQALGDRKGIA
                     RYGFTLPMDEANATVSIDLSGRPYPVFEGTFATPFIGDYRTDLTAHVFRSLAEAMGAA
                     VHVSVTGDDDHHKTEAVYKAFGRALRQAIRIEGDAVPSTKGVL"
     gene            complement(2092..2817)
                     /locus_tag="VF686_05240"
                     /pseudo
     CDS             complement(2092..2817)
                     /locus_tag="VF686_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(2814..4121)
                     /gene="hisD"
                     /locus_tag="VF686_05245"
     CDS             complement(2814..4121)
                     /gene="hisD"
                     /locus_tag="VF686_05245"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX8661251.1"
                     /translation="MKRFDWSALDAAGRAAALARPRQRTEARVTDTVRDILDDVAARG
                     GQAVTDWSVKLDGSPPRRIALTPDVVAAARDQLRPADTRSIRIAAENVRIFHEATRPE
                     DSDWVEITPGVRSRIVWRPLRSAGLYVPGGSAPLFSSLLMMAIPAAVAGVGRRVVVTP
                     PSKSGETHPAMVYAAAEAGLEALWLIGGAQAIAALTFGAELEDGVIEPYDKLFGPGNA
                     WVAEAKKQAAARPGGPAVDMPAGPSELLVIADRRADPVLAAADLLSQAEHDADAQVLL
                     VCDSERMINEILAEVDEQLPSLPRADIARASLAEGRAIRVLDLDMACTVANAYAPEHL
                     ALQTEDADDRVAKITAAGAVFLGAFAAETLGDYAAGPSHVLPTDGGARTLGGLTTASF
                     MTSMSVQSVTEAGAAELAPIAARLARLEGLEAHARAADLRTGE"
     gene            complement(4121..5002)
                     /gene="hisG"
                     /locus_tag="VF686_05250"
     CDS             complement(4121..5002)
                     /gene="hisG"
                     /locus_tag="VF686_05250"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX8661252.1"
                     /translation="MSTAQGRLRIAVQKSGRLSERSLDLVRDAGLRVVKGNNDLLYRV
                     ENYPIDLLRVRDDDIPTFVADGVCDLGIVGENVLEEVRNGGPVAEVLMPLGFGRCTLK
                     IATPPTLTYEGPASLAGLRIATSYPKILRRYLDAQGVQADIVTMRGAVEVAPRLKLAS
                     AICDLVSTGATLEANGLGAKETVLESQAVLIKSPVAPEPGLEHLLDSIVERMAGVVSS
                     QGAKYVMLNAPRSALDQITAILPGAGAPTVMPLSGRDDAVAVHAVCQEAVFWETLERL
                     KAAGASAILVLPIEKMM"
     gene            complement(5013..5327)
                     /locus_tag="VF686_05255"
     CDS             complement(5013..5327)
                     /locus_tag="VF686_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX8661253.1"
                     /translation="MVNPVSSKVLAKAADDRIALYDAILSLRTREELDAFLADLCTPS
                     ELRAFAERWAVARLLDAGGKSYREIAVEADASPTTVVRVARFLREMPHQGYRLVLDRI
                     KS"
BASE COUNT          797 a         1680 c         2124 g          752 t
ORIGIN      
        1 cgtcgcggac gtcgagacag gggatgatgc ggcgggcggt catgccgtcg ccgccagggc
       61 ctcggccacg gtgaagcggt tctcgtagag ggcgcggccg acgatgacgc cgtcgcatcc
      121 ggcgtcgcgg gcggcggtca ggtcggagag ttgggacacg ccgccggagg cctgaaccct
      181 caggtcgggg cggcggcggg cgatttcgcc cagcaggtcg atattgggac cggtcagggc
      241 gccgtcgcgg ccgacgtctg tgaccagcag gtggcgggcg aggccgggcg gatagcggtc
      301 cagagcggcc cacagatcga cctcggccgc ctcggtccag cccttgaggg cggggatcca
      361 gcggtcgccg tcggccttga cgtcgatggc cagcgtaatg cggtcggcgc cgaagcgggc
      421 cagccagtcg gccacggccc aagactgcga tacggccaga gagccgacga cgacgcgggc
      481 gacaccggcg tccagcaggg cctgcacgtc gtcggcggag cgcactccgc cgccggactg
      541 gatgcggatg tcgatcgccg cggccagttc gccgatcagg gcgtgctgga cggctcgtcc
      601 ggcctcggcg ccatccagat cgacgatgtg cgcccagatc gcgccgtcgg cggcgaaggc
      661 ggcgagacgc gcggcgggct ggtcgtcgta gcgggtcact tcgtcgaatt tgccgtgcat
      721 caggcgcacg caaacgcctt gtcgcaggtc gatggccggg tagacgatca tgacgtcacg
      781 ctcactgggg cagctcgaga aagttcttca ggatgcaggc gccggccgcc gccgaccgtt
      841 cggggtggaa ctggcaaccc cagcggttgc cggcgcggac gatcgccggc accggccctc
      901 cataatcggc gcaggcgagg gtcgccggtc cgtcgggaca gacgaagcca tgcacgaagt
      961 aagcgtaggc gccctcctca aggcgttcca gcagcgggtc gtccttgatc tgcgaaaggc
     1021 gcgaccagcc catgtggggc acgggcagac cggggccggg cgcaagggct gtgacgcggc
     1081 ccgggatcag gccgagcatg gccgttccgc cgccctcgtc gctggcttcg aacaacagct
     1141 gctggccgag gcagacgccg aggaggggac gctggaaggc ccggatgggc tcgaccaacc
     1201 ccagttcacg aaggcgcgcc atggcgtagg cggcggctcc cacgccgggc aggatcagac
     1261 gtcgggcgtc ggcgacttcg gcgggatcgg cggtcagccg cacggtggcg cccagccgtt
     1321 cgagcgcgaa caggaccgaa gcggtattgc cggcgccgta ggcgagaacc gtcacttccg
     1381 tcacagcacg cccttggtcg atggcacggc gtcgccttcg atgcgaatgg cctgacggag
     1441 ggcccggccg aacgccttgt agaccgcctc ggtcttgtgg tggtcgtcgt cgccggtgac
     1501 ggagacatgc accgccgcgc ccatggcttc ggccagcgag cggaagacgt gagcggtcag
     1561 gtcggtgcga tagtcaccga tgaagggggt ggcgaaggtt ccctcgaaga cagggtaggg
     1621 tcggccggac aggtcgatgg aaacggtggc gttggcttcg tccatgggca gggtgaagcc
     1681 gtagcgggcg atgcccttgc ggtcgccgag cgcctgtttc agcgcctgac cgagggcgat
     1741 ggccgagtcc tcgatggtgt ggtgcgggtc ggtgtgcagg tcgccctcgc aggcgagccg
     1801 cagcgagaag cctccgtggg tcgcgacctg ttccagcatg tggtcgaaga agccgacgcc
     1861 ggtggcgatg gagacgggac ccgtggcgtc gagatcgacg gcgcagacga tgcgggtctc
     1921 tttcgtgtcg cgaacagcct ggccggtgcg gtgcgcccgg cccgagccgg cgccgaggcc
     1981 gagggcggcc agcagccggt cgttcagcgc ggcgttggcg gagacgggca gacgaacgcg
     2041 gtcgccggcg cggtccgagg cggctccgag ccgggccagc gccgcagcga gagcgtgcgg
     2101 gtcggcaggg cgcgccatga tgacggggcc gaatccgggt tcgacggcca tgtcccggga
     2161 cagcgccttg accagccgtt cgcgctcgcg ccggacggcg gcgaggcgtt cgccgctctc
     2221 catcatccgc gacggatcga gcgcctgaag ggcgaggcgc accgagactt cgggcagggc
     2281 gtacggttcg agcaccgcgg caagacgggt gagagtttcg ggctgaccga gtgcggcccc
     2341 aacgcgggcg ccggcgagcc cgtaggccag cgaaaggctg cgcagcacga tcagattggc
     2401 gtggtcgccg atgacgctcg cggcggagag tgtgtcggcg aactcggcca gaccctcgtc
     2461 gacaatcagc agggcggggg cgacgcgttc ggccatctcg gccacggcct cgggcgagcc
     2521 gagagcgcgc aggatgaccg cggcgattgg tgcgccggca ggcggcgcgg cgtagagggc
     2581 gcgcagggtg cgatagggtt cggcgtcggg ggcctcgacc gcctgaccga ctcgcgcggc
     2641 gaggcggaag accagttcga ggccgtgggt caggccgcgc accggcagga cgctgtgggc
     2701 gggcacgcca tagacctcag ccatgcgggc gacgagggcg cggggctcgt ccggatagcg
     2761 gtcgaggccg tcgccgcccg cgaccagcgg ggcgaatgga gcggggagat tgttcattcg
     2821 cccgtcctca gatcggcggc gcgggcgtgg gcctccaggc cctccagccg ggcgagacgg
     2881 gcggcgatcg gggccagttc ggcggctccg gcctcggtga cgctctggac cgacatggag
     2941 gtcatgaagc tggcggtggt caggccgccg agcgtgcggg cgccgccgtc agtgggcagg
     3001 acgtggctgg gtccggcggc gtagtcgcca agcgtctcgg ccgcgaaggc gccgaggaag
     3061 accgctccgg cggcggtgat cttcgccacc cggtcatcgg cgtcctcggt ctggagggcg
     3121 aggtgttcgg gggcgtaggc gttggcgacc gtgcaggcca tgtcgaggtc gaggacacgg
     3181 atagcgcggc cttcggcgag tgaggcgcga gcgatgtcgg cgcggggcag ggacggcagc
     3241 tgctcgtcta cctcggcgag gatttcgttg atcatccgct cgctgtcgca gaccagcaga
     3301 acctgcgcgt ccgcgtcgtg ttcggcctgg ctgagcaggt cggcggcggc aaggaccgga
     3361 tcggcgcggc ggtcggcgat gaccagcagt tcggaggggc ccgcgggcat gtcgacggcg
     3421 gggccgccgg gacgggcggc ggcctgtttc ttcgcctcgg cgacccaggc gttgccgggg
     3481 ccgaacagct tgtcgtaagg ctcgatgacg ccgtcttcga gctcggcgcc gaaggtcagg
     3541 gcggcgatgg cctgggcccc gccaatgagc cacagcgcct cgagcccggc ctcggcggcg
     3601 gcgtagacca tggccgggtg cgtttcgccg gacttcgagg gcggggtgac gacgacccgg
     3661 cggccgacgc ccgcgacggc ggcgggaatt gccatcatca gcagggacga gaacagcggg
     3721 gcggaaccgc cggggacgta gaggcccgcc gatcgcagcg gacgccagac gatccgtgaa
     3781 cggactccgg gcgtgatctc gacccagtcg gagtcctcgg ggcgggtggc ctcgtggaag
     3841 atgcggacgt tctcggcggc gatacggatg gagcgggtgt cggccgggcg cagttgatcg
     3901 cgggccgccg cgacaacatc gggagtcagg gcgatgcggc ggggcgggga gccgtccagc
     3961 ttgacgctcc agtcggtgac ggcctgaccg ccgcgcgcgg cgacgtcgtc gaggatgtcg
     4021 cgcaccgtgt cggtgacgcg ggcctcggtg cgttggcgtg gccgggcgag ggcggcggcg
     4081 cggccggcgg cgtccagcgc gctccagtcg aagcgtttca tcacatcatc ttttcgatgg
     4141 ggaggacgag gatggccgag gcgccggcgg ccttgagccg ttccagcgtc tcccagaaga
     4201 cggcctcctg acagacggcg tgcacggcta cggcgtcgtc gcgacccgac aggggcatga
     4261 ccgtcggggc gcccgcgccg ggcaggatgg cggtgatctg atccagcgcc gagcgggggg
     4321 cgttgagcat cacgtatttg gccccttggg aggacacgac gccggccatg cgctcgacga
     4381 tgctgtccag caggtgttca aggccgggct ccggcgccac gggggacttg atcagcaccg
     4441 cctggctttc gaggactgtc tccttcgcgc cgaggccgtt ggcctcgagg gtcgcgccgg
     4501 tagagacgag gtcgcagatg gccgaggcca gcttcaggcg cggggcgacc tcgacggcgc
     4561 cgcgcatggt gacgatgtcg gcctgcacgc cctgtgcatc gagatagcgg cgcagaatct
     4621 tcggatagga ggtcgcgatg cgcaggccgg ccagcgaggc cgggccttcg tacgtcagcg
     4681 tgggtggcgt ggcgatcttc agcgtgcagc ggccgaaacc gagcggcatc aggacctcgg
     4741 cgacgggacc gccgttgcgg acctcctcca gcacgttttc gccgactatg ccgaggtcgc
     4801 agacgccgtc ggcgacgaag gtcgggatgt cgtcgtcgcg cacgcgcagc aggtcaatgg
     4861 gatagttctc gacccggtac agcaggtcgt tgttcccctt gacgacgcgc aggcccgcgt
     4921 cgcggacgag gtcgaggctg cgttcggaca ggcggccgga tttctgcacg gcgatgcgca
     4981 gccggccctg ggcggtactc atgctttttt actcaggatt tgatgcggtc gaggacgagg
     5041 cgatagccct gatgcggcat ctcccggagg aaccgggcga cgcggacgac cgtagtgggg
     5101 ctggcgtcgg cctcgacggc gatctcgcga taggatttgc cgccggcgtc gagcaggcgc
     5161 gcgacggccc agcgctcggc gaaggcgcga agttcggatg gggtgcagag gtcggccaga
     5221 aacgcgtcca gctcctcgcg cgtgcgcagc gacaggatcg cgtcatagag ggcgatgcga
     5281 tcgtcggcgg ccttggcgag gaccttgctg gagacggggt tgaccattca cgttccacta
     5341 agctgtaacg ctg
//
LOCUS       DASVVN010000325         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_356815,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000325 DASVVN010000000
VERSION     DASVVN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356815"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..>3104
                     /locus_tag="VF686_05260"
     CDS             39..>3104
                     /locus_tag="VF686_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8661254.1"
                     /translation="MCALAFPIATAGAQRGGGGAGPRADTANPIQEGLPLKPERTITF
                     TTDVGSWMSLDISPDGQTIVFDLLGDIYVMPVAGGEARAIASGVAWEMQPKWSPDGRH
                     IAFTSDRGGGDNIWIMDADGSNPTQVSKETFRLVNQPEWTPDGQFVVGRKHFTSSRSL
                     GAGEMWMYHRSGGGGVQMTERRTQQKDTGEPAFSPDGRYLYFSDDATPGTTFEYSKDV
                     NDQIYVIRRLDRETGDIDAYVTGPGGSIRPTPSPDGKSLAFIRRVRYQSTLYVMDIES
                     GRETPVFSALDRDMQETWAIHGVYPAISWTPDNRSIVFWADGRIQRVDVASKEVANIP
                     FHVADTRRVQDAVRFPIEVAPDQFDVKMLRWARPSPDGTKVVFEALGNLWIRDLPTGT
                     ARRLTPQSDHFELYPSWSRDGRSIVYTTWDDQELGSIRVVPASGGAGRVVTQNPGHYV
                     EPTFSPDGRTIVYRTTSDGFLTTALWSRDPGVYRIPTAGGEPVHITDEGESPQFGADG
                     DRLFLTGRDGDKRALISVDLDGDDKRTHLTSEWAAEFAVSPDGNWVGWTERFQAYVAP
                     FVAAGRPQAIAPDGKALPQARVTRDSGEWLSWSGDSRRLQWSQGPELFTRDLNQSFTF
                     VEGAPAELPKPAEQGINIGFTAEADKPEGRIALTGARIITMNGAEVIENGVIVIDGNR
                     IETIGSLASTTIPAGVPTMDMSGKTIMPGLVDAHWHGAMGSDEIIPEQSWVDYASLAF
                     GVTTIHNPSSDTSEIFAHSELARAGRVVAPRIFSTGSILYGATTAGTVKVESLDDALS
                     TLRRMQAAGAWSVKSYNQPRRDQRQQIIEAARQLNMMVVPEGGSLFQHNMSMIVDGHT
                     TIEHSIPVAVLYDDVHQLWRQTNVAYNPTLVVAYGGSYGENYWYQESEVWNHPILTQY
                     VPRRILDSRARRPETLPANEWNHISIAREARRLSDQGVSVSIGAHGQREGLAAHWELW
                     SLAQGGMAPLDIIRAGTLNGARSLGLDRDIGSLEPGKLADMVVLNANPLE"
BASE COUNT          514 a         1227 c          926 g          437 t
ORIGIN      
        1 cctgcatgac gtttcatcgt ccacttcggc tggccgcgat gtgcgcgctc gcctttccga
       61 tcgcgaccgc cggcgcccag cgcggcggtg gcggcgccgg cccgcgcgcc gacaccgcga
      121 atccgatcca ggagggcctg ccgctcaagc ccgagcgcac catcaccttc acgaccgacg
      181 tgggcagctg gatgtcgctc gacatcagcc ccgacggcca gaccatcgtc ttcgacctgc
      241 tgggcgacat ctatgtcatg cccgtcgccg gcggcgaggc tcgcgccatc gcctccggcg
      301 tggcctggga gatgcagccg aaatggtcgc ccgacgggcg ccacatcgcc ttcacctcgg
      361 accggggcgg cggcgacaac atctggatca tggacgccga cggctcgaac ccgacgcagg
      421 tgtccaagga gaccttccgg ctggtgaacc agcccgagtg gacgcccgac ggccagttcg
      481 tcgtcggccg caagcatttc acctcgtccc gctcgctcgg ggccggcgaa atgtggatgt
      541 accatcgtag cggtggcggc ggcgtgcaga tgaccgagcg ccgcacccag cagaaggaca
      601 cgggcgagcc cgccttctcg cccgacggcc gctatctgta cttcagcgac gacgccacgc
      661 cgggaacgac cttcgaatac tccaaggacg tcaacgacca gatctacgtc atccgccgcc
      721 tcgaccgcga gaccggcgac atcgacgcct atgtcaccgg ccccggcggt tcgatccgcc
      781 cgacgccctc gcccgacggc aagtccctgg ccttcatccg gcgcgtccgc taccagtcga
      841 ccctctatgt catggatatc gagtccggcc gcgagacccc ggtcttctcg gccctcgacc
      901 gcgacatgca ggagacctgg gccatccacg gcgtctatcc ggcgatcagc tggacgcccg
      961 acaaccgctc catcgtcttc tgggccgacg gccgcatcca gcgggtcgac gtggcctcga
     1021 aagaggtcgc caacatcccc ttccacgtcg ccgacacccg ccgggtgcag gacgccgtcc
     1081 gcttcccgat cgaggtcgcg cccgaccagt tcgatgtgaa gatgctgcgc tgggcccgcc
     1141 cctcgcccga cggcaccaag gtcgtcttcg aggccctcgg caatctgtgg atccgcgacc
     1201 tgccgaccgg cacggcgcgc cgcctgaccc cccagagcga ccatttcgaa ctctatccgt
     1261 cgtggtcgcg cgacggccgc tcgatcgtct acacgacctg ggacgatcag gagctgggct
     1321 ccatccgcgt ggtccccgcc tcgggcggcg cgggccgcgt cgtgacgcag aacccgggcc
     1381 actacgtcga gccgaccttc agccccgacg gccgcaccat cgtctatcgc accaccagcg
     1441 acggcttcct gaccaccgcc ctgtggagcc gcgaccccgg cgtctaccgc atccccacgg
     1501 cgggcggcga acctgtccac atcaccgacg agggcgagag cccgcagttc ggcgccgacg
     1561 gcgaccgcct gttcctcacc ggccgcgacg gcgacaagcg cgccctgatc tcggtcgatc
     1621 tcgacggcga cgacaaacgc acccacctga ccagcgaatg ggccgccgag ttcgccgtct
     1681 cgcccgacgg caactgggtc ggctggaccg agcgcttcca ggcctatgtc gctcccttcg
     1741 tggccgccgg tcgcccccaa gccatcgccc ccgacggcaa ggccctgccg caggcccgcg
     1801 tcacccgcga ctccggcgaa tggctcagtt ggtccggcga cagccgccgc ctgcaatggt
     1861 cgcagggtcc cgagctcttc acccgcgacc tgaaccagtc cttcaccttc gtggaggggg
     1921 cccccgccga acttcccaag ccggccgaac agggcatcaa catcggcttc accgccgagg
     1981 ccgacaaacc cgagggccgc atcgccctta ccggcgcccg catcatcacc atgaacggcg
     2041 ccgaggtgat cgagaacggc gtcatcgtca tcgacggcaa ccgcatcgag accatcggct
     2101 ccctcgcctc gaccaccatc ccggcgggcg ttccgaccat ggacatgtcg ggcaagacca
     2161 tcatgcccgg cctcgtcgac gcccactggc acggcgccat gggctcggac gagatcatcc
     2221 cggaacagag ctgggtcgac tacgcctcgc tggccttcgg cgtgaccacg atccacaacc
     2281 cgtccagcga caccagcgag atcttcgccc acagcgagct ggcccgcgcc ggccgcgtcg
     2341 tcgccccgcg catcttctcg accggctcca tcctgtacgg cgccaccacc gccggcacgg
     2401 tcaaggtcga gagcctggac gacgccctgt ccaccctgcg ccgcatgcag gccgccggcg
     2461 cctggagcgt caagagctac aaccagcccc gccgcgatca gcgccagcag atcatcgagg
     2521 ccgcccgcca gctgaacatg atggtcgtcc ccgagggcgg ctccctgttc cagcacaata
     2581 tgtcgatgat cgtcgacggc cacaccacca tcgagcattc gatccccgtc gccgtcctct
     2641 acgacgacgt ccaccagctg tggcgccaga ccaacgtcgc ctacaacccg accctcgtcg
     2701 tcgcctatgg cggcagctat ggcgaaaact actggtatca ggagagcgag gtctggaacc
     2761 acccgatcct gacccagtac gtcccgcgcc gcatcctcga cagccgcgcc cgccggcccg
     2821 aaaccctgcc ggccaacgaa tggaaccaca tctccatcgc ccgcgaggcc cgccgcctgt
     2881 ccgatcaggg cgtcagcgtc tccatcggcg cccacggcca gcgcgagggc ctcgccgccc
     2941 actgggagct gtggagcctg gcccagggcg gtatggcccc gctcgacatc atccgcgccg
     3001 gcaccctgaa cggcgcccgc tcgctgggcc tcgaccgcga catcggctcg cttgagcccg
     3061 gcaagctggc cgacatggtg gtgctcaacg ccaacccgct ggag
//
LOCUS       DASVVN010000326         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_356914,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000326 DASVVN010000000
VERSION     DASVVN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356914"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VF686_05265"
     CDS             <1..775
                     /locus_tag="VF686_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661255.1"
                     /translation="VWEPSFLPRFNLSIDYFNIKVEGAIQTLGALNTLDLCYNANDAA
                     ACSRINRNPGNGSLWVGDGNVEDLNINIGGFETSGVDFNANYSVDLEDWGAAGMGSLS
                     GSFVGTLLNELVTDTGAGTTESVYDCTGFYANQCGVPNPEWRHRARLTWSTPWDLDVS
                     GTWRHYGEVELAVLGAGGSLNNGGTRLDRYFDAENYFDLAATWQVMDTVTLRAGVNNV
                     FDNDPQLSYSVGTTGNGNTYPQLYDALGRYFFFGVTANF"
     gene            complement(772..930)
                     /locus_tag="VF686_05270"
     CDS             complement(772..930)
                     /locus_tag="VF686_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661256.1"
                     /translation="MISMKGAWNGERECRPATLEQGKWFLCGERADNKKGGGPEDPPP
                     QFVFDIRP"
     gene            920..1456
                     /locus_tag="VF686_05275"
     CDS             920..1456
                     /locus_tag="VF686_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661257.1"
                     /translation="MLITLVSALMLSTQTPPPAQDGGVVSTAPGAEAVETERFREAVA
                     YANPMPRGAPEGDYPLVAWCEALVNGHVALGETLTNRDPLDLDIIRLGKLEAADFRAA
                     LNAAEPRQTAASRAAARAAAAEATAKWAPLAGQEEAVRSQAFGLFFGLPGRCEHAARR
                     IRAGITTAPATPADVGLE"
     gene            complement(1754..>2886)
                     /locus_tag="VF686_05280"
     CDS             complement(1754..>2886)
                     /locus_tag="VF686_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX8661258.1"
                     /translation="DASRAVGVVSGLLSKTERAKNEAATRDEYVRIREQYARGQEVKA
                     RASLPAARQNRFRPDPATPLPPAPSFLGVRAFDSWDLGDLADHIDWTPFFASWELIGR
                     YPLILEDEIVGSAARDLFKDAQAMLKRIVDEKWFTARGVVGFWPANTVGDDIAVYADE
                     NRTEEIARFHTLRQQIRKSNGKPNLAMSDFIAETGRDYIGAFAVTAGHGELEIAKRFK
                     DAGDDYSSIMATALADRLAEAFAEALHKKVRTELWGHAPDEQTTVEDLIAERYQGIRP
                     APGYPAQPDHTEKATLFRLLQAGDNAGMALTESFAMTPPASVSGLYFGHAESHYFGVG
                     KIDPDQVEDYARRKGWDVATAERWLSPILNYDPGAPARSDAA"
BASE COUNT          458 a          995 c          946 g          487 t
ORIGIN      
        1 cgtctgggag cccagcttcc tgccgcgctt caacctctcg atcgactact tcaacatcaa
       61 ggtcgaaggc gccattcaga cgctgggcgc cctgaacacg ctggacctgt gctacaacgc
      121 caatgacgcg gccgcctgca gccgcatcaa ccgtaacccg ggcaacggtt cgctctgggt
      181 cggtgatggc aacgtcgagg acctgaacat caacatcggc ggcttcgaaa cctccggcgt
      241 cgatttcaac gccaactaca gtgtcgacct cgaggactgg ggcgccgccg gcatgggctc
      301 gctgtcgggt tcgttcgtcg gcaccctgct gaacgaactg gtcaccgaca cgggcgccgg
      361 cactacggag tccgtctatg actgcaccgg cttctacgcc aaccagtgcg gcgttccgaa
      421 cccggaatgg cgtcaccgcg cccgtctgac ctggtccacc ccgtgggacc tggacgtgtc
      481 gggtacgtgg cgtcactacg gcgaagttga actcgccgtc ctgggcgcgg gtggttcgct
      541 gaacaatggc ggcacccgtc tggaccgtta cttcgacgcc gagaactact tcgacctggc
      601 ggcgacctgg caggtgatgg ataccgtcac cctccgcgcc ggcgtcaaca acgtgttcga
      661 taacgatccg caactgtcct acagcgtggg tacgaccggc aacggcaaca cctatccgca
      721 gctgtatgat gcgctgggcc gttacttctt cttcggtgtc accgccaact tctagggcct
      781 gatatcaaag acaaactggg gcggcggatc ttcgggtccg ccgccctttt tgttgtccgc
      841 gcgctcgccg cacaaaaacc atttgccctg ttcaagcgtt gcaggccgac actcccgttc
      901 accgttccag gcgcccttca tgctgatcac cctcgtctcc gccctgatgc tctcgaccca
      961 gaccccgccg ccggcccagg acgggggcgt cgtctccacc gctccgggcg cggaggccgt
     1021 ggaaaccgag cgattccggg aggcggtggc ctacgccaac ccgatgccgc gcggcgcgcc
     1081 ggaaggcgat tacccgctgg tggcctggtg cgaggcgctg gtgaacggcc acgtggccct
     1141 gggcgagacc ctgaccaacc gcgatccgct ggatctggac atcatccgtc tgggcaagct
     1201 tgaagccgcc gacttccgcg cggcgctgaa cgccgccgag ccgcgccaga ccgccgccag
     1261 ccgcgccgcc gcgcgcgccg ccgccgccga agccacggcc aaatgggcgc cgctggccgg
     1321 tcaggaggag gcggtgcgca gtcaggcctt cggcctgttc ttcggcctgc ccggccgctg
     1381 cgaacacgcc gcgcgccgca tccgcgccgg catcacgaca gcgccggcga cgccggccga
     1441 cgtcggtctg gaataggcga ggaattggac gacggattcg cggagaattc gaagactcgc
     1501 cggagtgact tcggtgactt cagagaccgc ttgtcatccc ggacgccgcg agcggcgatc
     1561 cgggacggcg caacgctggt ttcgggctcc cggaaatcgc agcgcgattg tccgggagaa
     1621 agcctgccgc cacgctgcgt cggaacacat ctcccggaca gctgctgacg cagcttccgg
     1681 gaggttcaac ctgcgcactc catcgtcccg gatcgtcgct tcgcaacgtc cgggatgaca
     1741 aggcgcacgg acgtcaggcc gcgtcgcttc gcgcgggcgc gcccggatcg tagttgagga
     1801 tcggcgacag ccagcgctcg gccgtcgcca cgtcccaccc cttgcggcgg gcgtagtcct
     1861 cgacctgatc cggatcgatc ttgccgacgc cgaaatagtg actttcggca tgcccgaaat
     1921 acaggcccga caccgacgcc ggcggcgtca tggcgaagct ctcggtcagg gccatgccgg
     1981 cgttgtcccc ggcctgcagc aggcggaaca gcgtcgcctt ctccgtatgg tcgggctggg
     2041 ctggatagcc gggcgcgggg cggatgccct ggtaccgctc ggcgatcagg tcctcgaccg
     2101 tcgtctgctc gtccggtgca tggccccaca gctcggtccg gaccttcttg tgcagggcct
     2161 cggcgaaagc ctcggccaga cggtcggcca aggctgtcgc catgatcgag gaatagtcgt
     2221 ccccggcgtc cttgaaccgt ttggcgatct ccagctcgcc gtgaccggcg gtgacggcga
     2281 aggcgccgat atagtcccgg cccgtttcgg cgatgaagtc cgacatggcc agattgggct
     2341 tgccgttcga cttcctgatc tgctggcgca gggtgtggaa gcgcgcgatc tcttcggtgc
     2401 ggttctcgtc ggcatagacg gcgatgtcgt cgccgactgt gttggcgggc cagaagccga
     2461 ccacgccgcg cgccgtgaac catttctcgt cgacgatccg cttcagcatc gcctgggcgt
     2521 ccttgaacag gtcgcgcgcg gccgagccga cgatctcgtc ctcgaggatc agcggatagc
     2581 ggccgatcag ctcccagctg gcgaagaagg gcgtccagtc gatgtggtcg gccagatcgc
     2641 ccagatccca gctgtcgaaa gcgcgcacgc cgaggaatga cggggcaggg ggcaggggcg
     2701 tcgccggatc gggccggaag cggttctgcc gcgcggcggg cagggacgcc cgcgccttga
     2761 cctcttggcc gcgcgcatac tgttcgcgga tgcgcacgta ttcgtcgcgc gtggcggcct
     2821 cgttcttcgc ccgctcggtc ttcgacagca gtcccgagac cacgccgacg gcgcgcgagg
     2881 cgtcga
//
LOCUS       DASVVN010000327         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_326284,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000327 DASVVN010000000
VERSION     DASVVN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326284"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..159)
                     /locus_tag="VF686_05285"
     tRNA            complement(85..159)
                     /locus_tag="VF686_05285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(125..127),aa:Thr,seq:ggt)
     gene            259..441
                     /locus_tag="VF686_05290"
     CDS             259..441
                     /locus_tag="VF686_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661259.1"
                     /translation="MDESKTTGARPPLPTSLPDPLAPANTPAMARAQRLSTALRDNLR
                     RRKAAGRAVKPATPRN"
     gene            539..>1560
                     /gene="murA"
                     /locus_tag="VF686_05295"
     CDS             539..>1560
                     /gene="murA"
                     /locus_tag="VF686_05295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8661260.1"
                     /translation="MDSIAITGGARLNGSIPVSGAKNSALKLMAASILTDQPLLLTNM
                     PRLADTKFLGRLLRELGVEVTERDGADGQETLFHAPRITSTFAPYDLVRQMRASFNVL
                     GPLLARTGEAKVSLPGGCTIGARPVDLHLQALTALGAEIELDEGYVTARAPNGLKGAE
                     IEFPFVSVGATEHALLAAVLADGVTVLKRAAREPEIGDLAHCLTAMGAKISGIDTDEL
                     VITGVSSLGGASWSVIPDRIEMGSYAVAAAMAGGEVRLTKARPELMAALGEKMIEAGV
                     EVTPTADGVIVKRDPAVRLKAVDVATEVYPGFATDLQAQFMALMTTAEGDSVIHENIF
                     ENRFMHA"
BASE COUNT          270 a          530 c          534 g          226 t
ORIGIN      
        1 tttctttgag tcgcgcgccc tggccggacg ccgaaacgca aacggggcct gaccgcgaag
       61 tcaggccccg taagggtttc agagtggtgc cgccgggggg aatcgaaccc acgacctcag
      121 ccttaccaag gatgcgctct accactgagc tacggcggcc tgatctgagg aagcggccct
      181 atagccaacc gtggacaacg ggggaagccc cgatttcgaa tgcttgcgga aaagttcgac
      241 gcgggctctc ctcgacccat ggatgagtcg aagaccacag gggcccggcc cccgctgccg
      301 acaagcctgc cggacccgct ggcgccggcg aacacgcccg ccatggcgcg cgcccagcgg
      361 ctgtcgacgg cgctgcggga caatctgaga cgccgcaagg cggcgggcag ggcggtcaag
      421 ccggcaacgc cgcgcaactg aatgtcatcg ccgacacagc cgtcggcctt tcgcaaccgc
      481 aaccgtccgc gctagatagc caaccccctg caccccgcac ggggtcgcaa gagacgacat
      541 ggacagcatc gccatcaccg ggggcgcccg cctgaacgga tcgattccgg tcagcggcgc
      601 caagaactcc gccctcaaat tgatggccgc ctcgatcctg accgatcagc cgctgctgct
      661 gaccaacatg ccgcgtctgg cggacacgaa atttcttggc cggttgctgc gcgagctggg
      721 cgtggaggtg accgaacgcg acggcgccga cgggcaggag acgctgtttc acgcgccgcg
      781 gatcacctcg accttcgccc cctatgatct ggtccgccag atgcgcgcct cgttcaacgt
      841 tctgggcccg ctgctggcgc gcacgggcga ggccaaggtg tcgctgcccg gcggctgcac
      901 catcggcgcg cggcccgtcg atctgcacct gcaggcgctg acggcgctgg gcgcggagat
      961 cgagctggac gagggctatg tcacggcccg cgcgcccaac ggcctgaagg gcgcggagat
     1021 cgagtttccc ttcgtctcgg tcggcgcgac cgaacacgcc ctgctggccg ccgtgctggc
     1081 tgacggggtg acggtgctga aacgcgccgc gcgcgagccc gagatcggcg atctggccca
     1141 ctgcctgacc gccatggggg cgaagatcag cggcatcgac accgacgaac tggtcatcac
     1201 gggagtttcg tcgctgggcg gcgccagctg gtcggtgatt cccgaccgga tcgaaatggg
     1261 ctcctacgcc gtggccgccg ccatggccgg gggcgaggtg cggctgacca aggcccgtcc
     1321 cgagctgatg gcggcgctgg gcgagaagat gatcgaggcc ggggtcgagg tgacgccgac
     1381 cgccgacggg gtgatcgtca agcgcgaccc ggccgtgcgg ttgaaggcgg tcgacgtggc
     1441 gaccgaggtt tatccgggct tcgccaccga tctgcaggcc cagttcatgg ccctgatgac
     1501 gaccgccgag ggcgacagcg tcattcacga gaacatcttc gaaaaccgct tcatgcatgc
//
LOCUS       DASVVN010000328         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_511975,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000328 DASVVN010000000
VERSION     DASVVN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511975"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VF686_05300"
     CDS             complement(<1..477)
                     /locus_tag="VF686_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8661261.1"
                     /translation="MRHRSLLAGASAALALFAAVPALAEAPPETAAPAAAQPADAFDY
                     KDMISANRLADPQVSPDGRWVVYSVTTTDVAANRRTGSLYVMDLRDGGEGRRLAISEG
                     GANTARWGGDGKLYFLSGRSGSSQVWRAESDGARPVQVTDLPVDVNAYRLSRDASKV"
     gene            complement(554..>1603)
                     /locus_tag="VF686_05305"
     CDS             complement(554..>1603)
                     /locus_tag="VF686_05305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8661262.1"
                     /translation="ALRWRSDGKALYAVAAENGQQKLFEIDARTGAVRAVTDEGTVAG
                     FDETGGRLVLSQEGFGGPAQIVDASGEAPRALTQHNAAALAGTDLPEGESFTFPGWNG
                     EAVQGWVFKPAGYVEGRRYPAVYLIHGGPKSPWTHGWSYRWNPQIYTGAGYAVVMVNF
                     HGSPGFGQAFTDAINDHWGDRPLEDLQKGWAAALSNNAFIDGDRACALGASYGGYMVN
                     LIAGKWNGPWRCLVNHAGVFDVGQLMNAMDISTFVAEFGGPSWERADLYRDFSPNTFV
                     GDWSKPMLVLHGSRDFRVPVEQGLGTFSALQRRGIESRFVHVPDENHWVLKPRNWVDW
                     QQEILDWTADHTAAP"
BASE COUNT          244 a          571 c          545 g          243 t
ORIGIN      
        1 gaccttcgag gcgtcgcggc tcaggcgata ggcgttcacg tcgaccggca ggtcggtgac
       61 ctgcaccggc cgcgcgccgt ccgactcggc gcgccagacc tggctgctgc ccgagcggcc
      121 ggacaagaag tacaacttgc cgtcgccgcc ccagcgcgcg gtgttggcgc cgccctcgct
      181 gatcgcgagc cgccggcctt cgccgccgtc gcgcagatcc atgacgtaga gagaacccgt
      241 acggcggttc gccgccacgt cggtcgtggt caccgaatag accacccaac gcccgtccgg
      301 cgacacctgc ggatcggcca gccggttggc cgagatcatg tccttgtagt cgaaggcgtc
      361 ggccggctga gccgcggcgg gggccgccgt ttcgggcggc gcttcggcaa gggcgggaac
      421 ggccgcgaac agggccaggg cggcgctggc gccggcgagc agggaacggt gacgcatgga
      481 cggtttcctc ggtgactttg tcgtcaagtc tcggccgctg gcgtagcacc acgcggcgga
      541 gggcggaagc ggatcagggc gcggcggtgt gatccgccgt ccagtccagg atctcctgct
      601 gccagtcgac ccagttgcgc ggcttcagca cccagtggtt ctcgtccggc acatggacga
      661 accggctctc gatgccgcgc ctctgcaggg ccgaaaaggt ccccagcccc tgctccaccg
      721 gcaccctgaa atcgcgcgac ccatgcagca ccagcatcgg cttcgaccag tcgccgacaa
      781 aggtgttggg gctgaagtcg cggtacaggt ccgcccgctc ccaggacggg ccgccgaact
      841 cggcgacgaa ggtcgagatg tccatggcgt tcatcagttg gccgacgtcg aacaccccgg
      901 cgtggttgac cagacagcgc cacggtccgt tccacttgcc ggcgatcaga ttgaccatat
      961 agccgccgta cgaagcgccg agcgcgcagg cccggtcgcc gtcgatgaag gcgttgttcg
     1021 acagcgcggc cgcccagccc ttctgcagat cctccagcgg ccggtcgccc cagtggtcgt
     1081 tgatggcgtc ggtgaaggcc tgtccgaagc ccggcgagcc gtggaaattg accatcacca
     1141 ccgcatagcc ggcgcccgtg tagatctgcg ggttccagcg atagctccag ccgtgggtcc
     1201 aaggcgattt gggcccgccg tggatcagat agacggccgg atagcgccgc ccctcgacat
     1261 agcccgccgg cttgaacacc cagccctgca ccgcctcgcc gttccagccg gggaaggtga
     1321 agctttcgcc ttccggcagg tcggttccgg ccagcgcagc ggcgttgtgt tgcgtcaggg
     1381 cgcgcggcgc ctcgcccgag gcgtcgacga tctgcgccgg gccgccgaaa ccttcctgag
     1441 atagcaccag ccgcccgccg gtctcgtcga agcccgcaac cgtgccctcg tccgtcaccg
     1501 cccgcacggc gccggtgcgg gcgtcgattt cgaacagctt ctgctggccg ttctcggccg
     1561 cgacggcgta gagcgccttc ccgtcagagc gccagcgcag ggc
//
LOCUS       DASVVN010000329         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_433868,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000329 DASVVN010000000
VERSION     DASVVN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433868"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF686_05310"
     CDS             <1..380
                     /locus_tag="VF686_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661263.1"
                     /translation="KVMSRRHFATRFAQTAPRDQIDALYDRYVVPTAGKVYWDGILKP
                     EPITWRNPRRPPMLLIGGEVDLIADASMTRAIYEKQKQAPSATELKIFPGRSHWTGID
                     AGWEEVADHALDWALAHAPKQPA"
     gene            complement(377..778)
                     /locus_tag="VF686_05315"
     CDS             complement(377..778)
                     /locus_tag="VF686_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661264.1"
                     /translation="MTDNLLDEDGGGFDAEDWTRLKPFTGAVATLDDVQARRAVFALG
                     DTDDPRPIAMELPQPVIWWDDDGEQAAVAVQAETHLGPDGEEMEVLGLILPDGGGAVA
                     LMEDVDLVDASDPTWRDLVTSAIDPTAADED"
     gene            complement(788..2032)
                     /locus_tag="VF686_05320"
     CDS             complement(788..2032)
                     /locus_tag="VF686_05320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX8661265.1"
                     /translation="MTETLTIARVGGQGDGIADTADGPVFAPLTLPGEVVRGDLRDGR
                     LEGVEVLTASPERVAPVSPHYGDCGGCSLQHWAAGPYLDWKREQVRLALARERIETEI
                     EATIAVPPGTRRRLALHARRGEDGRVMLGFKARKSWRLVEVTACPVADPRLVAAFPAL
                     ARIAGAFLEHPKSAPTLHVTWTLTGLDVDVTGVERRSGGLSGDAQMRAIQAAAAADLA
                     RLSLAGETLMMERQPKVMFGAATVPLPAGGFLQAVPEAEAAMVERAVTATRGAKKIAD
                     LFCGAGTFTFPLAALAPVLAADGSAPGIAALKAGMGAAKGLKPITAEARDLFRRPLAP
                     YDLRGCDAVVLDPPRAGAIEQTKQLPGTKAGVVVYVSCNPVTFARDARVLIDAGFRLE
                     RVTPVDQFLWSAHVELVGVFRR"
     gene            2185..2835
                     /locus_tag="VF686_05325"
     CDS             2185..2835
                     /locus_tag="VF686_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661266.1"
                     /translation="MLARLTTKTAAAATALAAAGALLVPGAASAQTYSYQGPQNYGSG
                     AYDQRYDYDQSRYGDQRDYRGSYECRADRSSRQGGGLAIGATLGAVAGSQLAARGRRT
                     EGSILGGVLGAVIGAGVGGSSARDCGDSSGYGYRYDQSRYGDQARYGDGYGYQSYDRG
                     YDRDYDRYDRSYEDRYQSGYGDRSGYQTGCRTVEVRSRDYNGRYVTRYEQSCPDRY"
     gene            complement(3019..>3739)
                     /locus_tag="VF686_05330"
     CDS             complement(3019..>3739)
                     /locus_tag="VF686_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX8661267.1"
                     /translation="RLDQLLVARGLAESRSRAKAAIEAGGVTVDGEAAKAASQAVAED
                     AVVTYAGAHHWVGRGALKLDHALTLWPVAVEGRTVLDVGASTGGFTEVCLARGAARVF
                     AVDVGSGQMHARVAADPRVVNVEQTDARDLTPDLIPHPPELIVCDASFIGLAKVLPAA
                     LDLAAPGADLVTLVKPQFEASGPGGGKKGVVKDPAARQAALASVSEWLEAQGWSVQAT
                     AESPITGGDGNVEFLLWAKRR"
BASE COUNT          582 a         1401 c         1227 g          529 t
ORIGIN      
        1 tgaaggtaat gtcgcgccgg catttcgcga cccgcttcgc ccagaccgcc ccgcgcgatc
       61 agatcgacgc cctgtacgat cgctacgtcg tgcccacggc gggcaaggtc tactgggacg
      121 gcatcctgaa gccggagccg attacctggc gcaatccgag acggcccccg atgctgctga
      181 ttggcggcga ggtcgacctg atcgccgacg ccagcatgac tcgcgccatc tatgaaaagc
      241 agaagcaggc gccatcggcg accgagctga agatctttcc cggccggtca cactggaccg
      301 gcatcgacgc gggctgggaa gaggtggccg atcatgcgct cgactgggcc ctcgcccacg
      361 cgccgaaaca gccggcctag tcctcgtcag cggcggtcgg gtcgatggcg ctggtgacca
      421 gatcgcgcca ggttggatca gaggcgtcga ccagatcgac gtcttccatc agggccaccg
      481 ccccgccgcc gtcgggcagg atgaggccca ggacctccat ctcctcgccg tcgggaccca
      541 ggtgggtctc ggcctgcacc gccaccgccg cctgctcgcc gtcgtcgtcc caccagatca
      601 ccggctgcgg cagctccatg gcgatcgggc gcgggtcgtc ggtgtcgccc agagcgaaga
      661 cggcccggcg cgcctgaacg tcatccagcg tcgcaaccgc ccccgtgaaa ggcttgagcc
      721 gcgtccagtc ctcggcgtcg aagccgccgc cgtcctcgtc aagcagattg tcagtcatgg
      781 tcattgccta tcgtctgaaa acgcccacca gctcgacgtg ggctgaccac aggaactggt
      841 cgaccggcgt gacccgctcc agccggaaac cggcgtcgat caggacgcgc gcatcgcggg
      901 cgaaggtcac cggattgcag gacacataga cgacgacgcc cgccttcgtc cccggcagct
      961 gtttcgtctg ctcgatcgcc ccggcgcgcg gcggatccag cactacggcg tcgcagccgc
     1021 gcaggtcgta gggcgccagc ggccgccgga acaggtcgcg cgcctcggcg gtaatcggct
     1081 tcaatccctt ggccgcaccc atgccggcct tcagcgccgc aatgcccggc gccgatccat
     1141 ctgccgccag cacgggggcc agcgcggcca gcgggaaggt gaaggtcccg gccccgcaga
     1201 acaggtccgc gatcttcttc gcccccctcg tcgccgtgac ggcgcgctcg accatggcgg
     1261 cctcggcctc gggcacggcc tgcaggaagc cgcccgccgg caggggcacg gtcgcggccc
     1321 cgaacatgac cttcggctgg cgctccatca tcagggtctc gccggccagg ctgagccggg
     1381 cgaggtcggc cgccgccgcc gcctggatgg cgcgcatctg cgcatcgccg gacaggccgc
     1441 ccgagcgccg ctcgacaccg gtcacatcga cgtcgagccc ggtcagggtc caggtcacat
     1501 gcagggtcgg cgccgatttg ggatgctcca gaaaggcccc agcgatccgc gccagcgccg
     1561 ggaaggccgc gaccagccgg gggtcggcca ccggacaggc cgtcacctcg accagccgcc
     1621 agctcttgcg cgccttgaag ccgagcatga cgcggccgtc ctcgccgcgc cgcgcgtgca
     1681 gggccagccg cctccgggtc cccggtggga ccgctatggt cgcctcgatc tccgtctcga
     1741 tccgctcgcg cgccagggcg agccgcacct gttcgcgctt ccagtccaga tagggacccg
     1801 ccgcccagtg ctgcagcgaa cacccgccgc agtcgccgta gtggggtgaa accggggcga
     1861 cccgttcggg actggcggtc aggacttcga cgccctcaag ccgaccgtcg cgcaaatctc
     1921 cacgcaccac ctcgcccggc agggtcaggg gcgcgaacac aggcccgtcg gccgtgtcgg
     1981 cgatcccgtc gccctgcccg cctacgcgcg cgatcgtcag cgtttccgtc atggaccgtt
     2041 cctagaggct ccgaacggcg ggggaaacct gaacgaagat gaacggcctg ctcaaaagcc
     2101 gttcagcttc acccgcctat ttctcgatgc aacagacagg gacgccagcc gttggcctcg
     2161 tccctgcaac cgaggaccaa gccgatgctc gcccgcctga ccacgaagac cgccgcggcc
     2221 gccaccgcgc tcgccgccgc cggcgccctg ctggtccctg gcgccgcctc ggcccagacc
     2281 tacagctatc agggaccgca gaactatggt tcgggcgcct atgaccagcg gtacgactac
     2341 gaccagtccc gctacggcga ccagcgcgac tatcgcggca gctacgaatg ccgcgccgac
     2401 cgcagcagcc gtcagggcgg cggcctcgcc atcggcgcca cgctcggcgc cgtggccggc
     2461 tcgcaactgg cggcccgcgg ccgtcgcacc gaaggcagca ttctcggcgg cgtcctgggc
     2521 gcggtgatcg gcgccggcgt cggcggcagc tcggcccgcg attgcggcga cagcagcggc
     2581 tacggctacc gctacgacca gtcgcgctat ggcgatcagg ctcggtacgg cgacggctac
     2641 ggctatcagt cctacgaccg cggttatgac cgggactacg accgctacga tcgttcgtac
     2701 gaggaccgct atcagtccgg ctacggcgat cggtccggct atcagaccgg ctgccgcacg
     2761 gtcgaggtcc gcagtcgcga ctacaacggc cgctatgtca cccgctatga gcagtcctgc
     2821 ccggaccgct actaaatcac ggaattgcgt cgtcggttat ttttcagccc ccctgtcgat
     2881 cggcgacgta acggagaccc ccggtggagc aatccaccgg gggtttttcc tttttggggc
     2941 gctatcgcga gcgacgcggt cgctcgctgc ttgagcgtgg cgcgaagcgc cagaccgcgt
     3001 cgggtcgaaa aggatgggct agcgccgctt cgcccacagc aggaattcga cgttgccgtc
     3061 gccgccggtg atcgggcttt cggccgtggc ctgcactgac catccctgcg cctccagcca
     3121 ttcggagacc gaggccagcg ccgcctgacg ggccgcgggg tccttgacca cgcccttctt
     3181 gccgccgccg ggcccagacg cctcgaactg gggcttgacc agggtgacca ggtccgcgcc
     3241 cggcgccgcc agatcgagcg ccgccggcag caccttggcc aggccgatga agctggcgtc
     3301 acagacgatc agctccggcg gatgcggaat cagatcgggc gtcaggtcgc gggcgtccgt
     3361 ctgctccaca ttgacgacgc ggggatcggc cgcgacccgc gcatgcatct ggcccgagcc
     3421 gacatcgacg gcgaagacgc gcgccgcgcc gcgcgccaga cacacctccg tgaagccgcc
     3481 ggtcgaggcg ccgacatcca gcaccgtccg cccctcgacg gcgacgggcc aaagcgtcag
     3541 ggcgtgatcc agcttgagcg cgccgcgccc gacccagtga tgagccccgg cgtaggtaac
     3601 gaccgcgtct tccgccacgg cctgcgaggc ggccttcgcg gcttctccgt ccacggtcac
     3661 cccgccggct tcgatcgccg ccttcgcccg cgaccggctc tcggccagac cgcgcgcgac
     3721 cagcagctgg tcgagccgg
//
LOCUS       DASVVN010000330         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_326337,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000330 DASVVN010000000
VERSION     DASVVN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326337"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="gspL"
                     /locus_tag="VF686_05335"
     CDS             complement(<1..733)
                     /gene="gspL"
                     /locus_tag="VF686_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX8661268.1"
                     /translation="MKPTRLILIPALGSEPAPCLVIADGRVRERGVLELDALERPEPM
                     RTVAVAPGADVTIRWLDLPPGGPAQQRAAALWMLKVSLAAPTDRLAVALGAVPAAGQP
                     RLVAVVGLSLLQAWTDYLDALGVQADAIVPDALTVPEPDEDERLNAVAFGPAMALRGR
                     GFAASVQPDLVDLLAGLRRVTPIEDGDAVERSLIDAALSPPINLLSTQDRARGTSRRG
                     WALASALAGLLVISPLILIAAAAARD"
     gene            complement(730..>1620)
                     /gene="gspK"
                     /locus_tag="VF686_05340"
     CDS             complement(730..>1620)
                     /gene="gspK"
                     /locus_tag="VF686_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX8661269.1"
                     /translation="DVRFSVRRATNLETQSQAQWYAAGAETFARGLIDRLLTAGPART
                     PLEPAWNGRTLSFPIDGGSITAAVTDGQACFNLNSLVFGFGEDLTARPLGAAQFRALG
                     RAVGAPDSRMQAIANALTDWLDADAAALPLGAEDGAYAGLANPYRTGGVMLAEVSELR
                     AVKGVDAETYRRLRPYVCALPTTRLSPLNVNTLTPEQAPLLTMLTGGALGPERARAVI
                     AARPRDGWPDAAAFWAHPSLAAVQVPQEAREQITVLTRFFDLRVDVELGGSHAVRTAL
                     LEAGADGRVRTVIQRWTPEE"
BASE COUNT          220 a          561 c          635 g          204 t
ORIGIN      
        1 cgtcgcgggc ggcggcggcg gcgatcagga tcagcggcga gatcaccagc agtccggcga
       61 gggccgaggc caaggcccag ccccggcgcg aggtcccgcg ggcgcgatcc tgcgtcgaca
      121 ggaggttgat cggcggcgag agggccgcgt cgatcagcga ccgttcgacg gcgtcgccgt
      181 cctcgatcgg cgtgacgcgg cgcagacccg ccagcaggtc gaccaggtcg ggctggacgc
      241 tggcggcgaa gcccctgccc cgcaaggcca tggccgggcc gaaggcgacg gcgttgagcc
      301 gctcgtcctc gtcgggttcg ggcacggtca gggcgtcggg cacgatggcg tccgcctgta
      361 cgccaagggc gtccagatag tcggtccagg cctgaagcag ggacaggccg accacggcga
      421 cgagtcgggg ctggcctgcg gcgggcacgg cgcccagcgc cacagccagc cggtcggtcg
      481 gggcggccag cgacaccttg agcatccaca gggcggcggc gcgctgctgg gccggcccgc
      541 cgggcggcag atcgagccag cggatggtca cgtcggctcc gggcgcgacc gcgacggtgc
      601 gcatcggctc ggggcgttcg agggcgtcca gctccagcac gccgcgctcg cgcaccctgc
      661 cgtccgcgat caccagacag ggcgcgggtt cgcttccgag ggccggaatc aggatcagac
      721 gggtcggttt cattcttcag gggtccagcg ttggatcacg gtgcgcacgc ggccgtcggc
      781 tccggcctcc agcagcgcgg tgcggacggc gtgggagccg cccagttcga cgtcgacccg
      841 caggtcgaag aagcgggtca ggacggtgat ctgctcgcgc gcctcctgcg ggacctgaac
      901 ggcggccaga gacggatgcg cccagaaggc ggcggcgtcc ggccagccgt cgcgggggcg
      961 ggcggcgatc acagccctcg cccgttcggg cccgagcgcg ccgccggtca gcatggtcag
     1021 cagcggcgcc tgctccggcg tcagggtgtt cacattcagc ggcgacaggc gcgtcgtcgg
     1081 cagggcgcag acatacggcc gcaggcggcg gtaggtttcg gcgtcgaccc cctttacggc
     1141 gcgcagctcg ctgacctcgg ccagcattac gccgccggtc cgatacggat tcgccagccc
     1201 ggcataggcc ccgtcctcgg cgcccagcgg cagggccgcg gcgtcggcgt ccagccagtc
     1261 ggtcagggcg ttggcgatgg cctgcatgcg gctgtcaggc gcgcccaccg cgcggccgag
     1321 cgcgcggaac tgggctgccc ccagcggacg ggcggtcaga tcctcgccga agccgaacac
     1381 gaggctgttc agattgaaac aggcctgtcc gtcggtgacg gcggcggtga tgctgccccc
     1441 gtcgatgggg aagctcaggg tccggccgtt ccatgccggc tccagcgggg tgcgggccgg
     1501 gccggcggtc agcagccggt cgatcaggcc gcgcgcaaag gtttcggcgc cggcggcgta
     1561 ccactgggcc tggctttgcg tctccagatt ggtggcgcgg cgcaccgaga agcggacgtc
//
LOCUS       DASVVN010000331         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_433882,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000331 DASVVN010000000
VERSION     DASVVN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433882"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF686_05345"
     CDS             <1..208
                     /locus_tag="VF686_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX8661270.1"
                     /translation="ISSVIVVGGLIAAAAASADGGWIAKGAGVAAVTLASVNIFGGFM
                     VTRRMLAMYKKKERAAKPAEAAGS"
     gene            205..507
                     /locus_tag="VF686_05350"
     CDS             205..507
                     /locus_tag="VF686_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661271.1"
                     /translation="MTSAILFTAMVPLALLLVLGFVLLSGRMSQSLRKRVELGLILIV
                     YPALVVTWAWQAWDDQRAGDWAGFGLNLVLVVLFAIQFVIALRARTLFPRYGQPKE"
     gene            504..791
                     /locus_tag="VF686_05355"
     CDS             504..791
                     /locus_tag="VF686_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661272.1"
                     /translation="MTLDVHQFAVGASWVATAIGVGLWLWSWFGEKDAIRKLRFLDCG
                     VVLLFASVLLRVVAQDRPMSVWDWAMVFVAPLFIVAALWRLARTACPPGGR"
     gene            793..1179
                     /locus_tag="VF686_05360"
     CDS             793..1179
                     /locus_tag="VF686_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661273.1"
                     /translation="MRIVRIGALALLVAVAAPAAGAQTRLGADVESGLPWHVAALQGA
                     SWSLECRFKPVVYEASTYNREAWANRIAEIGRGSKGGRLPSDNGSCTVTKTGGRGSVA
                     IAMIKNGESQVAVTTDPARPAAVGFF"
     gene            1202..2620
                     /locus_tag="VF686_05365"
     CDS             1202..2620
                     /locus_tag="VF686_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8661274.1"
                     /translation="MNASFAALAYLVSGVLFILSLRGLSSPETSRQGNTFGMVGMALA
                     VGVTLLTLWSTGTLDPLTLALIAGGVIVGGGAGALIAARVQMTQMPQLVAAFHSLVGM
                     AACLVAVGAVYAPESFGIADGTGGIKIQSIIELALGVAIGAITFTGSVIAFAKLDGRM
                     SGAPILLPARHLINIGLALGLMLLIGIMIGTGGTATWAFWGVFLLALILGATLIIPIG
                     GADMPVVVSMLNSYSGWAAAALGFTLENIALIITGALVGSSGAILSYIMCKAMNRSFI
                     SVILGGFGGDTATGAAGAVETRPVKQGSADDAAFIMKNASKVIIVPGYGMAVAQAQHA
                     LREMADKLKEEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFSSCD
                     VAFVIGANDVTNPAAKTDPTSAIYGMPSLDVEKARTVLFVKRGMGSGYAGVENELFFR
                     DNTMMLFGDAKKVVDDILKAFD"
BASE COUNT          395 a          893 c          969 g          483 t
ORIGIN      
        1 catttcgtcg gtgatcgtgg tcggcggcct gatcgctgcg gcggcggcca gcgccgacgg
       61 cggctggatc gccaagggcg cgggcgtcgc ggccgtgacc ctcgccagcg tcaacatctt
      121 cggcggcttc atggtcaccc gaaggatgct ggccatgtac aagaagaagg aacgggcggc
      181 gaagcccgct gaggcggccg gatcgtgact tccgccatcc tcttcacggc catggtgccg
      241 ctggcgcttc tgctggtgct cggcttcgtt ctgctcagcg gacggatgag ccagagcctg
      301 cgaaagcggg tcgagctcgg tctgatcctg atcgtctatc ccgcccttgt cgtgacctgg
      361 gcctggcagg cgtgggacga tcagagagct ggtgactggg ccgggttcgg attgaatctg
      421 gtcctggtcg ttttgttcgc catccagttc gtcatcgccc tgcgcgcacg gacgctgttt
      481 ccccgctacg gacagccgaa ggaatgacct tggacgtcca tcagttcgcg gtcggcgcgt
      541 cctgggtcgc cacggcgatc ggggtcggcc tgtggctgtg gagctggttt ggcgagaagg
      601 acgcgatccg caagctgcgc ttcctcgact gcggcgtggt gctgctgttc gcctcagtgc
      661 tgctgcgcgt cgtggcgcag gacaggccga tgagcgtctg ggactgggcc atggtcttcg
      721 tggcgcccct gttcatcgtc gccgccctgt ggcggctggc gcggaccgcc tgtccgccgg
      781 gtgggcgttg agatgcggat cgtgaggatc ggagcgctgg cgctgctggt cgccgtcgcc
      841 gcgccggcgg cgggcgccca gacccggctc ggcgcagatg tcgagagcgg cctgccgtgg
      901 catgtcgcgg ccctgcaggg cgccagctgg tcgctggagt gccggttcaa gccggtggtc
      961 tacgaggcct ccacctacaa cagggaagcc tgggcgaacc ggatcgccga gatcggacgc
     1021 ggctcgaagg gcggccggct gccgtccgac aacggcagct gcaccgtgac taagactggc
     1081 gggcgcggct cagtcgccat cgccatgatc aagaacggcg agtcgcaggt ggcggtcacg
     1141 accgacccgg ccaggcccgc cgccgtggga tttttctgat gctgacactc ggggacacgg
     1201 catgaacgca tccttcgcgg ccctggccta tctggtctcc ggcgtgctgt tcatcctcag
     1261 cctgcgcggc ctgtcgagcc cggagacgag ccggcagggc aataccttcg gcatggtcgg
     1321 catggcgctg gcggtcgggg tgaccctgct gacgctgtgg agcacgggga cgctggatcc
     1381 gctgaccctg gcgctgatcg ccggcggcgt gatcgtcggc ggcggggcgg gggcgctgat
     1441 cgccgccagg gtgcagatga cccagatgcc ccagctcgtg gcggccttcc acagcctggt
     1501 cggcatggcg gcctgtctgg tcgccgtggg cgccgtctat gcgcccgaga gcttcggcat
     1561 tgccgacgga acgggcggga tcaagatcca gtcgatcatc gagctggcct tgggcgtggc
     1621 catcggcgcc atcaccttca ccggttcggt catcgccttc gccaagctgg acgggcggat
     1681 gtcgggcgcg ccgatcctgc tgccggcgcg gcacctgatc aacatcggcc tggcgctggg
     1741 gctgatgctg ctgatcggca tcatgatcgg cacgggcggg acggcgacct gggcgttctg
     1801 gggcgtgttc ctgctggccc tgatcctcgg cgcgaccctg atcatcccca tcggcggggc
     1861 ggacatgccg gtggtggtgt cgatgctcaa ctcctactcc ggctgggccg cggcggcgct
     1921 gggcttcacg ctggagaaca tcgccctgat catcaccggg gcgctggtcg gaagctcggg
     1981 cgcgatcctt agctacatca tgtgcaaggc gatgaatcgc agcttcatct cggtcatcct
     2041 tggcggtttc ggcggcgaca cggcgaccgg cgcggcgggc gcggtcgaga cccggccggt
     2101 caaacagggc agcgccgacg acgccgcctt catcatgaag aacgcctcga aggtcatcat
     2161 cgtgcccggc tatggcatgg ccgtggccca ggcccagcac gccctgcgcg agatggccga
     2221 caagctgaag gaggaggggg tcgaggtgaa atacgccatc caccccgtcg ccggccgcat
     2281 gcccggccac atgaacgtgc tgctggccga ggccaacgtc ccctatgacg aggtgttcga
     2341 gctggaggac atcaactcgg agttctcgag ttgcgacgtg gccttcgtca tcggcgccaa
     2401 cgacgtgacc aacccggccg ccaagaccga cccgacgagc gccatctatg gcatgccaag
     2461 tctcgacgtc gaaaaggccc gcaccgtgct gttcgtcaag cgcggcatgg gctccggcta
     2521 cgccggcgtc gagaacgagc tgttcttccg cgacaacacc atgatgctct tcggcgacgc
     2581 caagaaggtc gtcgacgaca tcctcaaggc cttcgactga ccgcgcgacg aggggcggcg
     2641 tgcgcaccgc cctgacgctc ctcgcattcc tggtcgccgc gtacgcggcc gtagtcctgc
     2701 tcttctacct gaagcagggc gcgttgctct atccggcgtc
//
LOCUS       DASVVN010000332         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_433904,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000332 DASVVN010000000
VERSION     DASVVN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433904"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VF686_05370"
     CDS             <1..463
                     /locus_tag="VF686_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX8661275.1"
                     /translation="FLRTRLDAVGPEAEAPLDGIARAGYRLTYVDDLTPVEKRIQSGW
                     ATDFQYSLGFNLNAANRITGVRWGSLAFDKGLGAGWDLIAVGDRTASAEVLRDAVTAA
                     RGGSGPIVLVVKRGDEFRTLSFDYHDGLRYPRLIRTEGRDRLADILAPRRR"
     gene            complement(829..1215)
                     /gene="rplL"
                     /locus_tag="VF686_05375"
     CDS             complement(829..1215)
                     /gene="rplL"
                     /locus_tag="VF686_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX8661276.1"
                     /translation="MADLAKIVEDLSKLTVLEAAELSKLLEEKWGVSAAAPVAMAMPA
                     GGGAGAPAEAAEEQTEFTVVLLDGGDKKINVIKEVRGVRSDLGLKEAKDLVEGAPQNV
                     VENVSKQAADEVAKKLTEAGAKVQVK"
     gene            complement(1282..1797)
                     /gene="rplJ"
                     /locus_tag="VF686_05380"
     CDS             complement(1282..1797)
                     /gene="rplJ"
                     /locus_tag="VF686_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX8661277.1"
                     /translation="MDRAQKAESIETLKGVFADAGAVVVTHNLGLTVADMEDLRGRLR
                     KEGGTFKVVKNRLALKALDAAEGSDYHGLFKGPVGIAYADDAVTAAKITAEYAKANDK
                     FVVLGGFMGEKILDAKGVEVLSKLPSLDQIRASLLGLLNAPATKIAGVVQAPASQLAR
                     VFSAYASKDAA"
BASE COUNT          287 a          623 c          567 g          343 t
ORIGIN      
        1 cttcctgcgc acgcgtctgg acgccgtcgg ccccgaggcc gaggccccgc tcgacggcat
       61 cgcccgcgcc ggctaccgcc tgacgtacgt tgacgatctg accccggtcg agaaacgcat
      121 ccagtccggc tgggccaccg acttccagta ttcgctcggc ttcaacctca acgccgccaa
      181 ccgcatcact ggcgtccgtt ggggtagtct cgccttcgac aaggggctgg gcgccggctg
      241 ggatctgatc gccgttggcg accgcactgc ctcggccgag gtcctgcgcg acgcggtcac
      301 cgccgcgagg ggcggctccg gacccatcgt cctcgtcgtc aaacgcggcg acgagttccg
      361 caccctgtcc ttcgactacc acgacggcct gcgctacccg cggctgatcc ggaccgaagg
      421 ccgcgaccgc ctcgccgaca tcctcgcacc gcggcggcgg tagtcctttt gtcatcccgg
      481 ccgccgcaaa gcggcgagcc gggacgaaca gatccacccc atactcctcc cggaaatcgc
      541 atcgcgatta tccgggagac agggggcccc accctgtctc ccggatcgcc gctgcgcagc
      601 gtccgggagc gccggatcgc gagtgcaaag cgtcccggat caccgctgcg cggcgtccgg
      661 gatgacaagg gtagtcatcg ccttttctga ccgaacagca gccggtccag tgccgtccat
      721 gcaccccccc gaattggagt tcacgcccaa agaaaaaacg ggccggagat cgctccccgg
      781 cccgttcatt ttcagcgtac cgcccgaggg cggcagagcc cggtctgctt acttgacctg
      841 gaccttggcg ccggcttccg tcagcttctt ggcgacttcg tcggcggctt gcttggaaac
      901 gttctcgacg acgttctgcg gagcgccttc gaccaggtcc ttggcttcct tcagacccag
      961 gtccgaacgc acgccacgga cttccttgat gacgttgatc ttcttgtcgc cgccgtccag
     1021 caggacgacg gtgaactcgg tctgctcttc agcagcttcg gcgggagcgc cggcgccgcc
     1081 gccggcgggc atggccatgg cgaccggagc agcggcgctg acgccccact tttcttccag
     1141 cagcttggac agttcggcgg cttccagaac ggtcagcttc gacaggtctt caacgatttt
     1201 ggcgagatcg gccatttggg ttttccttcg gaggatgggt tttcagagag agttttgcag
     1261 agatgaaaga ggcggttgcg cttacgcggc gtccttcgag gcgtacgcgc tgaacacgcg
     1321 ggccagttgg gaagccgggg cttgcacgac gccggcgatc ttggtcgcgg gagcgttgag
     1381 caggccgagc agcgaagcgc ggatctggtc cagcgacggc agtttggaga gaacctccac
     1441 gcccttcgcg tccagaatct tctcacccat gaagccgccc agaacgacga acttgtcgtt
     1501 ggccttggcg tattcagcgg tgatcttggc ggccgtcacc gcgtcgtcgg cgtaggcgat
     1561 gcccacggga cccttgaaca ggccgtggta gtcgctgcct tcggcggcat ccagcgcctt
     1621 gagcgccagg cggttcttga ccaccttgaa cgtgccgcct tctttacgaa ggcgtccacg
     1681 cagatcttcc atgtccgcaa cggtcagacc caggttgtgg gtcacgacca cggcgcccgc
     1741 gtcggcgaaa acgcccttca gcgtttcgat cgactcggct ttttgtgcgc ggtccattgc
     1801 ggtctccagt ctggtattcg
//
LOCUS       DASVVN010000333         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_248189,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000333 DASVVN010000000
VERSION     DASVVN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248189"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="lspA"
                     /locus_tag="VF686_05385"
     CDS             <1..489
                     /gene="lspA"
                     /locus_tag="VF686_05385"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8661278.1"
                     /translation="AVTAFVFALLVVVLDQLTKAWVLSGLDLREVGRVNILPPILNFT
                     WVENRGVSFGLFGDGSARWMLSLFSIIVSGILGWWALRADRRLLITAIGLVMGGAIGN
                     VIDRIRFGFVVDFIDFSGTGVFPWVFNVADSAITIGVILLILDSLLSERAAKVGLAAE
                     KS"
     gene            553..1116
                     /locus_tag="VF686_05390"
     CDS             553..1116
                     /locus_tag="VF686_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEX8661279.1"
                     /translation="MRLRTVAVLTLATSMLALGACSSVRQGIGLTKITPDEFLTVSTA
                     PLTVPPEYGLRPPAPGQPRPQELAPESAARQILLGQRQAVTRSPGEQQLVAQAGGDRA
                     DPLARYVVDDEFGDLAHKEEGWANRLLFWRREDPASQIATTTQTSEGAVTVDAATEYA
                     RLQELTGGRTGIVIAQRRDTGFKLPGL"
     gene            complement(1176..>1556)
                     /locus_tag="VF686_05395"
     CDS             complement(1176..>1556)
                     /locus_tag="VF686_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8661280.1"
                     /translation="IPPLKMQGMLALVALPTLAFGSAMFERGQWQAVQQADALIWTCV
                     VWAGLVSSVFATTLVFWLVQKREAGRVTPYLLATPVVSILIGWGFMGDVLTPQILTGA
                     ALTMGGVAIVALAERGLRAGAAKA"
BASE COUNT          246 a          581 c          480 g          249 t
ORIGIN      
        1 gccgtcacgg ccttcgtctt cgcccttctc gtcgtggtgc tcgaccagct gaccaaggcc
       61 tgggttctgt ccggcctcga tctgcgcgag gtgggacggg tcaacatcct gccgccgatc
      121 ctgaacttca catgggtgga aaaccgcggc gtcagcttcg gcctgttcgg cgacggctcg
      181 gctcgctgga tgctgagcct cttctccatc atcgtttccg gcatcctggg ctggtgggcg
      241 ctgcgggccg accgccggct gctgatcacc gccatcggcc tggtcatggg cggcgcgatc
      301 ggcaacgtca tcgaccgcat ccgcttcggc ttcgtcgtcg acttcatcga tttttcgggc
      361 accggggtct ttccctgggt gttcaacgtc gccgacagcg ccatcaccat cggcgtcatc
      421 ctgctgattc tggacagcct gctgtccgaa cgggcggcca aggttggcct cgccgccgaa
      481 aagtcgtaat cagccgcatc attcaaggct acgcgcgcgc ccgctccggc gcttcgcccc
      541 gagatcacat acatgcgcct tcgtactgtc gccgtcctga ccctcgccac ctcgatgctc
      601 gcgctcggcg cctgctcgag cgtccgtcag gggatcggcc tgaccaagat tacgcccgac
      661 gagttcctga cggtgtcgac cgccccgctg accgtgccgc cggaatacgg cctgcgtccg
      721 cccgcgccgg gccagccgcg cccgcaggag ctggctcccg agtccgccgc ccgccagatc
      781 ctgctgggtc agcgtcaggc cgtgacccgc tcgcccggcg agcaacagct ggtggcccag
      841 gccggcggcg accgcgccga tcccctcgcc cgctacgtcg tcgacgacga gttcggcgat
      901 cttgcacaca aggaagaggg ctgggccaac cgcctgctgt tctggcgccg tgaagacccc
      961 gccagccaga tcgcgaccac gacccagacc tcggaaggcg ccgtcaccgt cgacgccgcc
     1021 accgaatatg cgcgcctgca ggaactgacc ggcggccgca ccggcatcgt catcgcgcaa
     1081 cgccgcgata cgggcttcaa gcttccgggc ctctaggaca caccgccaga tcccgaaaaa
     1141 ggcccggatg cgaaagcgtc cgggcctttt gcttgtcagg ccttcgccgc cccggcccgg
     1201 aggccgcgct cggccagggc cacgatggcg acgccgccca tggtcagggc cgcgccggtc
     1261 aggatctggg gcgtcagcac atcgcccatg aaaccccatc cgataaggat cgacacgacg
     1321 ggtgtggcca acagataggg cgtcacccgg cccgcctccc gcttctgcac cagccagaac
     1381 accagggtcg tggcgaacac cgacgagacc agccctgccc aaacgacaca ggtccagatc
     1441 agggcgtcgg cctgctggac cgcctgccac tgaccgcgtt cgaacatcgc ggagccgaac
     1501 gccagcgtcg gcaaggccac gagcgccagc atgccctgca tcttcagcgg cgggat
//
LOCUS       DASVVN010000334         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_275995,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000334 DASVVN010000000
VERSION     DASVVN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275995"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF686_05400"
     CDS             <1..886
                     /locus_tag="VF686_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEX8661281.1"
                     /translation="FIATPHLMLGGLETGAGVEIGITALWAVAPFFGVVMLATILGGV
                     GGNVAQSGLIFTAEKLKPDWSKVNPMAGFKRIFGPDGLVQFLKTFIKLLAVGFICWLV
                     LKPHLRDLENMAAMPPAMILPAARDLILALVTSTLILLGFTAGADFLWQKFRFAERMK
                     MTKEEIKEDYKQSEGDPHVKAKLKQIRAQRSRQRMMQNVPKATVIVTNPTHYSVALRY
                     EPGEGDPAPVCVAKGVDALALRIREMGREHGVPIVENVPLARALYAAVDVDETIPREH
                     FEAAAKVIGFVMQKRKKR"
     gene            982..2631
                     /gene="fliF"
                     /locus_tag="VF686_05405"
     CDS             982..2631
                     /gene="fliF"
                     /locus_tag="VF686_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX8661282.1"
                     /translation="MGGFTAALQRFGIGRLTVVLGVAAGITIALILIMMRVGQAPDAL
                     LYSNLDLQEASEVTTALDSAGIKYSSKGDGSTIFVSRDQVGEARMLVAGKGLVTSGSV
                     GYEIFDNQSVLGQTEFQQNLNEKRALEGELSRTIMSMRGINSARVMIALPRRELFQSD
                     AADPTASIVVGVTGGQLSGAQVQAIRNVVASSVPNLKPEKVTLTDTSNRTLAAGSDAE
                     GFSAASAQDAQETTESRLQARIKDIVEGVVGVGGARVQVTADIDHSRSTTQELRYDPD
                     GQVVRSTSTNGTESSDTTPTSDGGATATNNIPGGAAPETGTAGSRDTANTETTNYEIS
                     NTTTTTVKEAGEIKRLAVSVAVDGKWTPGANGAAPTYAARTPAEIEQIKALVSAAVGI
                     DAARGDRIEVLNTRFNRDLAVVGGEEAKSSLFDFTKNDLMRGVELLVLLITGVLLIFF
                     VLRPLLKSASGGAPGGAMQLAGALPGGGGSVTSLQTTTVTGGGGPMHQLTGPASDMEH
                     RLDIARIEGQVKASSVKKVADFVDKHPDESTSILRSWVHEG"
     gene            2643..3668
                     /gene="fliG"
                     /locus_tag="VF686_05410"
     CDS             2643..3668
                     /gene="fliG"
                     /locus_tag="VF686_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX8661283.1"
                     /translation="MVSKKAAVEDARKLSGPEKAAVILLSLGEDHTRLWQSLDDEEIK
                     EISQAMASLGTVTSHVVEELMVEFVSGMSGSGAVMGSFEQTQRLLASFMPSERVEGLM
                     EEIRGPAGRTMWDKLGNVNEAVLANYLKNEYPQTVAVVLSKIKPDHAARVLTSLPEDF
                     ALECVQRMLRMEPVQRDILDKIEQTLRTEFMSNLARTSKRDSHEMMADIFNSFDRQTE
                     ARFIGALEERNREAAERIRALMFVFEDLAKLDPGGIQTLLRGVEKDSLALALKGSSES
                     LRELFFSNMSERASKIMREDMESMGPVRLKDVDQAQMAMVQVAKDLAAKGEIMLVGQG
                     GDDELIY"
     gene            3670..>3957
                     /locus_tag="VF686_05415"
     CDS             3670..>3957
                     /locus_tag="VF686_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FlbE"
                     /protein_id="HEX8661284.1"
                     /translation="MTHQPLTTPFAFDTEFDASGVVVHASAFRPTKRSYAPLEVDALI
                     AQARTEAREEALAESANVQALALSAIAQALDIAIPALTQVAHAHREQSAELA"
BASE COUNT          705 a         1404 c         1262 g          586 t
ORIGIN      
        1 cttcatcgcg acgccccatc tgatgctggg cgggctggag acgggggccg gcgtcgagat
       61 cggcatcacc gccctgtggg ccgtggcgcc cttcttcggc gtggtcatgc tggccaccat
      121 cctcggcggc gtcggcggca atgtcgccca gtccggcctg atcttcaccg ctgaaaagct
      181 gaagccggac tggtccaagg tgaatcccat ggccgggttc aagcgcatct tcggccccga
      241 cggcctggtg cagttcctca agaccttcat caagctgctg gccgtcggct tcatctgctg
      301 gctggtgctg aagccgcatc tgcgcgacct cgagaacatg gccgccatgc cgccggcgat
      361 gatcctgccc gccgcgcgcg acctgatcct ggcgctggtg acgtcgaccc tgatcctgct
      421 cggcttcacc gccggggccg atttcctgtg gcagaaattc cgcttcgccg agcggatgaa
      481 gatgaccaag gaagagatca aggaagacta caaacagtcc gagggcgacc cgcacgtcaa
      541 ggcgaagctg aagcagatcc gggcccagcg cagccgccag cggatgatgc agaacgtgcc
      601 caaggccacg gtcatcgtca ccaacccgac ccactattca gtcgccctgc gctacgagcc
      661 gggcgagggc gatccggccc cggtctgcgt cgccaagggc gtcgacgccc tggccctgcg
      721 catccgcgag atggggcgcg agcacggcgt cccgatcgtc gagaacgtgc ctctggcccg
      781 cgccctctac gccgccgtcg atgtggacga gaccatcccc cgcgagcatt tcgaggcggc
      841 cgccaaggtc atcggcttcg tcatgcaaaa gcggaagaag cgttgatcgt tcaagactct
      901 taacccggca tttaccatgc gcccggcatt ttctgcctgg cgggcgtcag tcagtacccg
      961 cgagtattcg gggcgggacg cgtgggcggc ttcacggcgg cgttgcagag attcgggatc
     1021 ggtcggctga ccgtggtgct gggcgtcgcc gccggcatca cgatcgcgct catcctgatc
     1081 atgatgcggg tgggccaggc ccccgacgcc ctgctgtact ccaacctcga cctgcaggaa
     1141 gcgtccgaag tcaccaccgc gctcgacagc gccggcatca aatattcctc gaagggcgac
     1201 ggttcgacca tcttcgtcag ccgcgatcag gtgggcgagg cccgcatgct ggtcgccggc
     1261 aaggggctcg tgacctcggg cagcgtcggc tacgagatct tcgacaatca gtcggtcctc
     1321 ggccagaccg agttccagca gaacctcaac gaaaagcgcg ccctcgaggg cgagctgtcg
     1381 cgcaccatca tgagcatgcg cggcatcaat tcggcccgcg tcatgatcgc cctgccgcgc
     1441 cgcgaactgt tccagtccga cgccgccgat cccaccgcct cgatcgtcgt cggcgtcacc
     1501 ggcggccagc tgtcgggcgc ccaggttcag gccatccgca atgtcgtcgc ttcgtcggtc
     1561 ccgaacctga agcccgagaa ggtcaccctc accgacacct ccaaccgcac cctcgccgcc
     1621 ggctcggacg ccgagggctt cagcgccgcc tcggcgcagg acgcgcagga gacgacggaa
     1681 tcccgcctgc aggcgcgcat caaggacatc gttgagggcg tcgtcggcgt cggcggcgcg
     1741 cgggttcagg tcacggccga catcgaccac agccgctcga ccacccagga actgcgctac
     1801 gaccccgacg gccaggtcgt ccgctcgacc tcgacgaacg gcacggagtc cagcgacacc
     1861 accccgacct cggacggcgg cgcgaccgcg accaacaaca tccccggcgg cgcggcgccc
     1921 gaaaccggaa cggccggatc gcgcgacacc gccaacaccg aaacgaccaa ctacgaaatc
     1981 tccaacacca ccaccacgac ggtgaaggaa gccggcgaga tcaaacgcct ggccgtctcg
     2041 gtggccgtcg acggcaaatg gacgccgggc gccaacggcg cagccccgac ctacgccgcc
     2101 cgcacccccg ccgagatcga acagatcaag gcgctggtct cggccgccgt cggcatcgac
     2161 gcggcgcgcg gcgacaggat cgaggtgctg aacacccgct tcaaccgcga tctggccgtg
     2221 gtcgggggcg aggaggccaa gtcctcgctg ttcgacttca cgaagaacga cctgatgcgc
     2281 ggcgtggagc tgctggtcct gctgatcacc ggcgtcctgc tgatcttctt cgtcctgcgt
     2341 ccgctgctga agtcggcgtc gggcggcgct ccgggcgggg cgatgcagct ggcgggcgcc
     2401 ctgccgggcg gcggcggttc ggtcacctcg ttgcagacca ccacggtcac gggcggcggc
     2461 gggccgatgc accagctgac cggcccggcc tcggacatgg agcaccgcct ggacatcgcc
     2521 cggatcgagg gtcaggtgaa ggcgtcctcc gtcaagaagg tcgcggactt cgtcgacaag
     2581 caccccgatg aatcgacctc gatcctgcgc agctgggtcc acgaaggctg atggtcgccc
     2641 gcatggtcag caagaaggcg gcggtcgagg acgcaagaaa gctgagcggc cccgagaagg
     2701 ccgccgtcat cctgctgtcc ctcggcgagg accacacccg cctgtggcag agcctggacg
     2761 acgaggagat caaggaaatc tcccaggcga tggccagcct ggggaccgtc acgtcgcacg
     2821 tcgtcgagga gctgatggtc gagttcgtct cgggcatgag cggctcgggc gcggtgatgg
     2881 gcagcttcga acagacccag cgcctgctgg cctccttcat gccgtccgag cgggtcgaag
     2941 ggctgatgga ggagatccgc ggtcccgccg gccggaccat gtgggacaag ctcggcaatg
     3001 tgaacgaggc cgtgctcgcc aactatctga agaacgaata cccccagacc gtcgcggtgg
     3061 tcctgtccaa gatcaagccg gaccatgcgg cccgcgtcct gaccagcctg cccgaggatt
     3121 tcgccctcga atgcgtccag cggatgctgc gcatggagcc cgtccagcgc gacatcctcg
     3181 acaagatcga acagacgctg cgcaccgaat tcatgtcgaa cctggcccgc acctcaaaac
     3241 gcgacagcca cgagatgatg gccgacatct tcaacagctt cgaccgccag accgaggctc
     3301 gcttcatcgg cgcgctggag gaacgcaacc gcgaggcggc cgagcgcatc cgcgccctga
     3361 tgttcgtctt cgaggacctg gccaagctgg accccggcgg catccaaacc ctgctgcgcg
     3421 gcgtcgagaa ggattccctg gccctggccc tcaagggctc ctcggagtcc ctgcgcgaac
     3481 tgttcttctc caacatgtcc gaacgcgcgt ccaagatcat gcgcgaggac atggaatcga
     3541 tggggcccgt ccgcctcaag gacgtcgacc aggcccagat ggccatggtc caggtcgcca
     3601 aggatctcgc cgccaagggc gagatcatgc tggtgggcca gggcggcgac gacgagctga
     3661 tctactgaca tgacccatca gccgctcacc acccccttcg ctttcgacac cgagttcgac
     3721 gcctccggcg tggtcgtcca cgcctcggcc ttccgtccga ccaaacgctc ctacgccccg
     3781 ctcgaggtcg acgccctgat cgcccaggcc cgcaccgagg cccgcgagga agccttggcc
     3841 gagagcgcga acgttcaggc cctggccctg tccgccatcg cacaggcgct ggacatcgcc
     3901 atccccgccc tgacgcaggt ggcgcacgcc caccgcgaac agtccgccga gctggcg
//
LOCUS       DASVVN010000335         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_171084,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000335 DASVVN010000000
VERSION     DASVVN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171084"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VF686_05420"
     CDS             <1..274
                     /locus_tag="VF686_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX8661285.1"
                     /translation="QAAAGVLGEGVIGASLDELAAARDAMSAMLKAGGEGPTGRFEGL
                     RALKQVADYPARHASTMVAIEATLDAMRQAHARKGPDTRTSLAGAA"
     gene            363..614
                     /gene="yidD"
                     /locus_tag="VF686_05425"
     CDS             363..614
                     /gene="yidD"
                     /locus_tag="VF686_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX8661286.1"
                     /translation="MASGGTLYERGVRLAHRGYKLTLSPLIGQSCRFRPTCSDYGRDA
                     LIQHGPLRGGWLTVRRLCKCHPLSEGWKYDPVPPAKTKR"
     gene            611..>1907
                     /gene="thrS"
                     /locus_tag="VF686_05430"
     CDS             611..>1907
                     /gene="thrS"
                     /locus_tag="VF686_05430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX8661287.1"
                     /translation="MIELKFPDGAARQYPDDATGRDVAASISPSLAKRAALVALNGEQ
                     RDLDRPLGRSGDFKLIMRDDPEALETIRHDAAHVLAQAVQELFPGTQVTIGPAIEDGF
                     YYDFHREEPFSTDDFAAIEKKMAEIVDRDEKLVREVWEREDAIRHFESVGETFKAELI
                     RDLPGTEEITVYRSGGWLDLCRGPHFPTTKFVGKAFKLTKLAGAYWRGDHRNPQLQRI
                     YATAWASKEDLDAYLLRVEEAEKRDHRKIGKAMGLFHMQEEGRGMVFWHPKGWELWRV
                     LEAYMRRRLDAAGYVEVKTPQVLDRTFWEKSGHWEKYRPNMFVCETVEGETLSLKPMN
                     CPGHVQIFDQGQRSYRELPLRMAEFGACHRYEPSGALHGLMRVRGFTQDDAHIFCRED
                     QIVDETRRFIELTKVVHADLGMETAYINLATRPEVRAGSD"
BASE COUNT          329 a          643 c          653 g          282 t
ORIGIN      
        1 ccaggccgcg gccggagtgc tgggggaagg cgtgatcggc gcctcgcttg acgaactggc
       61 tgcggcgcgc gacgccatgt cggccatgct caaggctggc ggcgagggcc cgacgggtcg
      121 gttcgagggc ctgcgcgccc tcaaacaggt ggccgactac cccgcgcgcc acgcctcgac
      181 catggtcgcg atcgaggcga cgctggacgc gatgcggcag gcccacgccc gcaaggggcc
      241 ggatacgcga actagcctcg ccggcgctgc atagcgccgg cttttcttcc tggcctaccg
      301 cgcttcgcgc tacttgaggc caggttgctc ctgcgcccat tgcaggggat aagcgcggcg
      361 acatggcttc cggcggaact ctctatgaac gcggcgtgcg gctggcccat cgcggctaca
      421 agctgacgct gtcgcccctg atcgggcagt cgtgccggtt ccggccgacc tgctccgact
      481 acgggcggga cgcgctgatc cagcacggac cgctcagggg aggatggctc acggtcagac
      541 gcctctgcaa atgccatcct ctcagcgagg gctggaaata cgatccggtc ccgcccgcaa
      601 agacgaaacg atgatcgaac tcaaattccc cgacggcgcc gcccgccagt atcccgacga
      661 cgccacgggc cgcgacgtcg ccgcctccat ctcgccgtcg ctggccaagc gcgcggcgct
      721 ggtcgccctg aacggcgagc agcgcgatct ggaccggccg ctgggcaggt ccggcgactt
      781 caaactgatc atgcgtgacg atcccgaggc gctggagacc atccgccacg acgccgccca
      841 cgtactggcc caggcggtgc aggagctgtt ccccggcacg caggtgacca tcggccccgc
      901 gatcgaggac ggcttctatt acgacttcca ccgcgaggag cccttctcga cggacgactt
      961 cgccgccatc gagaagaaga tggccgagat cgtggaccgc gacgagaagc tggtgcgcga
     1021 ggtctgggag cgcgaggacg cgatcagaca tttcgagagc gtcggcgaga cgttcaaggc
     1081 cgagctgatc cgcgatcttc cggggacgga ggaaatcacc gtctatcgct cgggcggctg
     1141 gctggacctg tgccgcggcc cgcatttccc gacgacgaaa ttcgtcggca aggccttcaa
     1201 gctgaccaag ctggcgggcg cctactggcg cggcgaccac cgcaatccgc agctgcagcg
     1261 catctacgcc acggcctggg cctccaagga ggatctggac gcctatctgc tgcgcgtcga
     1321 ggaggccgag aagcgggacc accgcaagat cggcaaggcc atgggcctgt tccacatgca
     1381 ggaggaaggc cggggcatgg tcttctggca tcccaagggc tgggagctgt ggcgcgtgct
     1441 ggaggcctac atgcgccgcc ggctggacgc cgccggctat gtcgaggtca agacgccgca
     1501 ggtgctggac cgcaccttct gggagaagtc ggggcactgg gagaagtatc gccccaacat
     1561 gttcgtctgc gagacggtcg agggcgagac gctcagccta aagccgatga actgcccggg
     1621 gcatgtgcag atcttcgacc agggccaacg gtcgtaccgc gaactgccgc tgcgcatggc
     1681 cgagttcggg gcctgccacc gctatgagcc gtcgggcgcc ctgcacggtc tgatgcgggt
     1741 gcgcggcttc acccaggacg acgcccacat cttctgccgc gaggaccaga tcgtcgacga
     1801 gacgcgccgg ttcatcgagc tgaccaaggt ggtccacgcc gacctcggca tggagacggc
     1861 gtacatcaat ctggccaccc ggcccgaggt gcgcgccggt tcggacg
//
LOCUS       DASVVN010000336         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_37556, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000336 DASVVN010000000
VERSION     DASVVN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37556"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..690)
                     /locus_tag="VF686_05435"
     CDS             complement(115..690)
                     /locus_tag="VF686_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX8661288.1"
                     /translation="MRLARLSALAALPLIAALGACVSVTANDYSDAPERATIGDFGEA
                     TLMNTSGARVGRAILTQGATGLLIRIEAEGLTPGWHGVHIHATGQCAAPFTSAGGHIN
                     HGEPKAPHGLLNAGGPDDGDLPNVFADARGRVNAEVFTTRARIAREGPGQWLWDADGS
                     ALVIHANMDDQTSQPIGGAGDRVVCGVMAAG"
     gene            816..>2109
                     /locus_tag="VF686_05440"
     CDS             816..>2109
                     /locus_tag="VF686_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8661289.1"
                     /translation="MTDDSYNDALPPVSGGGGGDISTITIEDELRRSYLDYAMSVIVS
                     RALPDARDGLKPVHRRILYSMHDLKMTPDRAYSKCARVVGDVLGRFHPHGDASVYMAL
                     VRMAQPFSMGLMLVDGQGNFGSVDGDMPASMRYTEARMAPAASALLTDIDKDTVDFQP
                     NYDEKELEPVVLPARIPNLLVNGAGGIAVGMATNIPPHNLDEIIDACVALLDDPNVSD
                     DALLDIVPGPDFPTGGEIMGRTAPRNALRDGRGSVMVRGVATIETIRKDREAIVVTQL
                     PFQVNKQTLIERIVEMVREKRIEGISDVRDESDRDGMRMVIELKRDASGDVVLNQLWR
                     FTAMQSSFGVNMLALNHGRPQQMGLRQLLEAFLEFREEVVVRRIKFELAKARDRGHVL
                     VGLAVAVANIDEVIHIIRSSADPAEARERLQAKSWPVGD"
BASE COUNT          338 a          713 c          721 g          337 t
ORIGIN      
        1 gaacaccaat gtctgggccg tcgaggtccg gcctgccgaa atcttcgcct acgaactgcg
       61 gcggccgaat cgctatctgc gcgtcgaatt cgacctgacg cggccggttg cggactagcc
      121 cgccgccatc acgccgcaga ccacgcggtc gccggcgccg ccgatgggct ggctggtctg
      181 gtcgtccata ttggcgtgga tgaccagcgc cgagccgtcg gcgtcccaaa gccactggcc
      241 gggcccctcg cgggcgattc gggcgcgggt ggtgaagacc tcggcgttca cccggccccg
      301 ggcgtcggcg aagacgttgg gcaggtcgcc gtcgtcggga ccgccggcgt tgagcagacc
      361 gtgtggcgcc ttgggctcgc cgtggttgat gtggccgccc gccgaggtga agggcgcggc
      421 gcactggccc gtggcgtgga tgtggacgcc gtgccagccg ggggtcaggc cctcggcctc
      481 gatacggatc agcaggcccg tcgccccttg cgtcaggatg gcccggccga cccgggcgcc
      541 gctcgtgttc atcagcgtgg cctcgccgaa atcaccgatc gtggcccgct ccggagcgtc
      601 tgaatagtca tttgcggtga cgctgacgca ggcccccagg gcggcgatca ggggcagggc
      661 ggcgagggcg gagaggcggg cgaggcgcat ggtctggaat ccttgtctgg acggagcgtt
      721 tccgggcggc ccggctttgc cacggagggc tctggatgct agaaaacccg ttcgcggatc
      781 acgttccgat tccggccatt taaagcctga tttccttgac tgacgacagc tataacgacg
      841 cgcttccccc ggtctcgggc ggcggcggcg gcgacatttc caccatcacc atcgaagacg
      901 agctgaggcg ctcgtatctc gactacgcca tgagcgtgat cgtgagccgc gccctgccgg
      961 acgcgcgtga cgggctgaag cccgtgcacc gccgcatcct gtattcgatg cacgacctga
     1021 agatgacgcc ggaccgcgcc tattcgaaat gcgcccgcgt cgtcggcgac gtgctgggcc
     1081 gtttccaccc gcacggcgac gcctcggtct atatggcgct ggtgcgcatg gcccagccgt
     1141 tctcgatggg tctgatgctg gtcgacggcc aaggcaattt cggctcggtc gacggcgata
     1201 tgccggcctc gatgcgatac accgaggccc gaatggcccc ggccgcctcg gccctgctga
     1261 ccgacatcga caaggacacc gtcgatttcc agccgaacta tgacgagaag gagctggagc
     1321 ccgtcgtcct gccggcgcgg attccgaacc tgctggtcaa tggcgcgggc ggcatcgccg
     1381 tcggcatggc gaccaacatc ccgccgcata acctcgacga gatcattgac gcctgcgtgg
     1441 ccctgctgga cgatccgaac gtcagcgacg acgccctgct ggacatcgtg cccggtcccg
     1501 acttcccgac cggcggcgag atcatgggcc gcaccgcgcc ccgcaacgcc ctgcgcgacg
     1561 gccgcggctc ggtcatggtg cgcggcgtcg ccaccatcga gacgatccgc aaggaccgcg
     1621 aagccatcgt ggtcacccag ctgccgttcc aggtcaacaa acagaccctg atcgagcgca
     1681 tcgtcgaaat ggtccgcgag aaacggatcg agggcatcag cgatgtccgc gacgagtccg
     1741 accgcgacgg catgcggatg gtcatcgagc tgaagcgcga cgcgtccggc gacgtggtgc
     1801 tgaaccagct gtggcgattt accgccatgc agagctcgtt cggcgtcaac atgctggcgc
     1861 tgaaccacgg ccgcccgcag cagatgggcc tgcgccagct gctggaagcc ttcctggagt
     1921 tccgggaaga ggtggtggtc cgccgcatca agttcgaact ggccaaggcc cgcgaccgcg
     1981 gccacgtgct ggtcggtctg gccgtggccg tggccaacat cgacgaggtc atccacatca
     2041 tccgcagttc ggccgatccg gccgaggcgc gcgagcggct tcaggccaag agctggcccg
     2101 tcggcgaca
//
LOCUS       DASVVN010000337         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_357246,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000337 DASVVN010000000
VERSION     DASVVN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357246"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF686_05445"
     CDS             <1..253
                     /locus_tag="VF686_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661290.1"
                     /translation="LHRNTIVWEGPVGPGQRTAEPPARWLYLVANPAVVLEISDLISD
                     PASRRTFSVPAERTVRSSRRNSFIGFTDNLNTPLPIQRR"
     gene            complement(260..>1843)
                     /locus_tag="VF686_05450"
     CDS             complement(260..>1843)
                     /locus_tag="VF686_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX8661291.1"
                     /translation="CVADATNLRLVLRLVLELKAVGRPMVLALNMYDIARRQGLRIDL
                     ERLSAELGVPVVPTTATRKRGIDDLIATVSSVLDAAPQTAASAWAAPDAAALRTAARE
                     AERIMKACVRPPERPDTLTGRIDSILLHPVGGLVILLGLLFVMFQAVFTWATPLMDGI
                     EGLLAALGVAVATVMPDGVWESLIVDGLISGVGSVLVFLPQILILFLFIILLEDFGYM
                     ARAAFLMDRVMGGAGLHGRAFIPLLSSFACAIPGVMAARVIDSKRDRLTTIMVAPLMT
                     CSARIPVYTLIIAAFIPNQQVWGFASLQGLVMFGLYASGIVSALAVSFVIRKVFWRGA
                     VEPFMMELPAYKTPDMKSVLFNLWIRARIFLERAGRIILPLMIIVWALATFPYPPEGA
                     TLPAIDYSFAGMIGHALEPLFAPIGFNWQMVIALVPGMAAREVAVAALGTVYAVADPE
                     SATSLLGATLAAQWSLATGLAFLAWYVFAPQCVATLGVVKRELNSWKWMWVMVVYMFG
                     LAYLAAFVVYRIAVALGGG"
BASE COUNT          345 a          591 c          659 g          248 t
ORIGIN      
        1 tctgcatcgc aacaccatcg tgtgggaggg gccggtggga ccggggcaac ggaccgcaga
       61 accgccggcg aggtggctgt atctggtggc caatccagcc gtcgttctcg aaatttccga
      121 cctgatcagc gatccggcgt cacgccgaac tttctccgtg ccggccgagc gaaccgtcag
      181 atcatcccga cggaacagtt ttatcgggtt caccgataac ttgaacacgc cgctcccgat
      241 ccagcggcgt tagcgtccct cagccgcccc cgagagcgac cgcgatgcgg tagacgacga
      301 aggccgccag ataggccagg ccgaacatgt agacgaccat cacccacatc catttccacg
      361 agttcagctc gcgcttgacc acgcccaggg tcgccacgca ctggggcgcg aagacgtacc
      421 aggcgaggaa ggcgaggccc gtcgccagcg accactgggc cgccagggtc gcgcccagca
      481 ggctggtggc gctctcggga tcggcgacgg cgtagacggt gcccagggcc gccacggcga
      541 cctcgcgggc ggccatgccg gggacgaggg cgatgaccat ctgccagttg aagccgatgg
      601 gggcgaacag cggctccagc gcgtggccga tcatgcccgc gaagctgtag tcgatggccg
      661 gcagggtcgc gccctcgggc ggatagggga aggtggcgag ggcccacacg atgatcatca
      721 gcggcaggat gatccggcct gcccgctcga ggaagatgcg ggcccggatc cacagattga
      781 acaggacgct cttcatatcg ggcgtcttgt aggccggcag ttccatcatg aagggctcga
      841 ccgcgcccct ccagaagacc ttgcggatga cgaaggagac cgcgagggcg ctgacgatgc
      901 cggaggcgta gaggccgaac atcaccagcc cctgcaggct ggcgaagccc cagacctgct
      961 ggttgggaat gaaggcggcg attatgaggg tgtagaccgg gatacgcgct gaacaggtca
     1021 tcagcggggc gaccatgatg gtggtcagcc ggtcgcgttt gctgtcgatc acccgcgccg
     1081 ccatgacgcc gggaatggcg caggcgaagc tcgagagcag gggaatgaag gcgcggccgt
     1141 gcagtcccgc cccgcccatg acccggtcca tcaggaaggc ggcgcgggcc atgtagccga
     1201 agtcttccag caggatgatg aacaggaaca ggatcaggat ctgcggcagg aagaccagca
     1261 cgctgccgac gcccgagatc aggccgtcga cgatcaggct ttcccagact ccgtcgggca
     1321 tgaccgtggc caccgcgacg cccagcgccg ccagcaggcc ttcgatgccg tccatcaggg
     1381 gcgtggccca ggtgaagacg gcctggaaca tcacgaacag caggcccagc aggatgacca
     1441 gcccgccgac cggatgcagc aggatggagt cgatccggcc ggtcagggtg tcgggccgct
     1501 cgggcgggcg gacgcaagcc ttcatgatcc gctcggcctc gcgggcggcg gtgcgcaggg
     1561 cggcggcgtc gggagcggcc caggcgctgg cggcggtctg cggagcggcg tccagaacgc
     1621 tggagacggt cgcgatgagg tcgtcgatgc cgcgcttgcg ggtcgccgtg gtgggcacaa
     1681 cgggcacgcc cagttcggcc gacagccgct cgagatcgat gcgcaggccc tgacgccggg
     1741 cgatgtcgta catgttgagc gccagcacca tcggccggcc gacggccttc agctccagca
     1801 ccagacgcag aacgaggcgc aggttggtgg cgtcggcgac gca
//
LOCUS       DASVVN010000338         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_406467,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000338 DASVVN010000000
VERSION     DASVVN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406467"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1350
                     /locus_tag="VF686_05455"
     CDS             310..1350
                     /locus_tag="VF686_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8661292.1"
                     /translation="MTLRGNRRFRPDRRAVIAGAGALAAVAAVSACQRSEAPVDEEGR
                     VRLRFATDWRAQAEHGGFYQALASGAYARRGLNVQIIQGGPGVNVPQLLASGAVELGM
                     GSNSFIPLNLVAEGAPVKAVAAFFQKDPQVLIAHPDPALRSIADLQGRPFLLADASVT
                     AFWVWLKAKYGFTDDQVRKYTFNLAPFLADERAVQQGYLTSEPYTIEQEAGFTPKVFL
                     LADEGYPSYATMVLAPDAFARDNAAALRSFIAGSAEGWRDYIQGDGKAADALIRKDNP
                     EVTQAILDQARAKLKANGIVDGGDAALYGLGAMTAERWQAFFDVTSGAGVYPAGLDWR
                     KAFTTQYLPGRG"
     gene            1343..>1573
                     /locus_tag="VF686_05460"
     CDS             1343..>1573
                     /locus_tag="VF686_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8661293.1"
                     /translation="MAEPTAALAGAVVRYPGRPPLGPIDLSISPGEIVALVGASGAGK
                     STVLRLLAGLERPSEGQDDPAAGAGRTGFVFQA"
BASE COUNT          237 a          588 c          508 g          240 t
ORIGIN      
        1 agaagaccgg agggaaccgg atcgggatcg atggacggcg cggggctcga cttcgtggaa
       61 cgccggagac caggagcgcg acaggataca gctgaccatc gccggggttc gcctcgctga
      121 tgctgaagcc agggccctga acctcaatgg ccaagtccaa cccggacttg gcgaggggac
      181 gaggaaaacc ggttcgccgg ggatcttcgt cccctcgctc aattcgggcg ggccgcaagg
      241 ttccgccctc tggcgcgcac cagttgaacg ccccttcccc ttctcccggc cgagccgtaa
      301 accaaggtca tgaccttgcg tgggaatcgc cgtttccggc cagaccgccg cgccgtgatc
      361 gcgggcgccg gcgcgctggc ggccgtggct gcggtctcgg cctgccagcg gtcggaggct
      421 cccgtcgacg aggagggccg cgtccgcctg cgtttcgcca ccgactggcg cgcccaggcc
      481 gagcatggcg gcttctacca ggccctcgcc tcgggggcct acgcccgccg cggcctgaac
      541 gtccagatca tccagggcgg gcccggcgtg aacgtgcccc agttgctcgc ctcgggcgcc
      601 gtcgaactgg gcatgggctc caacagcttc atccccctga accttgtcgc cgagggcgcg
      661 ccggtgaagg ccgtcgccgc cttcttccag aaggaccccc aggtcctgat cgcccatccc
      721 gatccggcgc tgagatccat agccgatctt cagggccgcc cgttccttct ggccgacgcc
      781 tcggtcacgg ccttctgggt ctggctgaag gccaaatacg gcttcaccga cgatcaggtg
      841 cgcaaataca ccttcaacct cgcccccttc ctggccgacg agcgggcggt gcagcagggc
      901 tatctgacct ccgagcccta tacgatcgag caggaggcgg gcttcacgcc caaggtcttc
      961 ctgctggccg acgagggcta tccctcctac gccaccatgg tcctggcgcc cgacgccttc
     1021 gcccgcgaca acgccgccgc cctgcgcagc ttcatcgccg gatcggctga gggctggcgc
     1081 gactacatcc agggcgacgg caaggcagcc gacgccctga tccgcaagga caatccggaa
     1141 gtgacccagg ctatactgga tcaagcccgg gccaagctga aggccaatgg catcgtcgac
     1201 ggcggcgacg cagccctcta cggcctcggc gccatgaccg ccgaacgctg gcaggccttc
     1261 ttcgacgtca cctcgggcgc cggcgtctac ccggcgggcc tggactggcg caaggccttc
     1321 accacccagt acctacccgg ccgtggctga gccgacggcc gcgcttgcgg gcgccgtcgt
     1381 tcgctacccc ggccgtccgc cgctcggccc catcgacctg tctatctcgc ccggagagat
     1441 cgtcgccctg gtcggtgcct cgggcgcggg caaatccacc gtcctgcggt tgctggccgg
     1501 gctggaacgg ccctccgaag gccaggatga ccccgccgcc ggcgccggcc ggaccggctt
     1561 cgtcttccag gcc
//
LOCUS       DASVVN010000339         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_171393,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000339 DASVVN010000000
VERSION     DASVVN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171393"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..802
                     /locus_tag="VF686_05465"
     CDS             356..802
                     /locus_tag="VF686_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8661294.1"
                     /translation="MNPALFSMIAVVLAGGATALQAPTNAKLMGAVGSPVNAAFVSFA
                     VGTAALGIVAMVMQTRPDISAARALPWYAWVGGLYGAIFVVAAAWGVPRLGVAMTITL
                     MVAGQLLVGLILDHFGAFGMPQQPISLGRAAGVLLVLAGVLMVRRF"
     gene            819..>1643
                     /locus_tag="VF686_05470"
     CDS             819..>1643
                     /locus_tag="VF686_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX8661295.1"
                     /translation="MTEETLPADSPPTPLVAADLIRDEARRAPDKPGVYRMYGEDGTC
                     LYVGKARSIKKRILQYAQGRFHTQRIGLMVSLTRSMELVITASETEALLLESSFIKQL
                     KPRFNVVLRDDKSFAELMIRRDHRAPQVRKHRGAHTTPGDYFGPFASTWAVNRTLNTL
                     QKAFLLRSCSDSVYETRTRPCMLHQIKRCSAPCTGLISLEDYGVLVDEASAFLKGKSR
                     AVIARLSAEMSAAAEAMDYEQAARVRDRIRALSAISMENSVSAEGVAEADVFALFSE"
BASE COUNT          253 a          597 c          507 g          286 t
ORIGIN      
        1 tgtcccttcg tgtccttcgt gacgaaccag ctctcgccga cacaccacag agcccggtga
       61 ttatcctcag aaccgccgca ccatcagcac cccggcgatc accagcgcga cgccggcggc
      121 gcgaccgagg ctgatcagca tacacgccaa tgagcgcttt tttcgcgggg atcacgtgct
      181 gctctaccgt atcgaccgct tttcgacggc ggaacggacc catcgcaacg aaatcgcgga
      241 tatcggttgg tttcatccgg aggccctgcc tgccgatact gaccctggaa cgcgagcgcg
      301 gatatcagag gtcttcgccg gtgcagcgtc tgcgacaagc tggtaacggg aaacaatgaa
      361 ccccgccctc ttctccatga tcgccgtcgt cctcgcgggc ggcgccaccg cccttcaggc
      421 tcccaccaat gcgaaattga tgggcgccgt cggctccccg gtgaacgccg ccttcgtctc
      481 cttcgccgtc ggcacggcgg cgttgggcat tgtcgccatg gtcatgcaga cccggccgga
      541 catctctgcg gcaagggcgt tgccttggta cgcctgggtc gggggcctgt acggcgcgat
      601 cttcgtcgtc gccgcagcct ggggcgtgcc gcggctcggc gtcgccatga ccatcaccct
      661 gatggtcgcc ggccagctgc tggtcggcct gatcctcgac catttcggcg ccttcggcat
      721 gccgcagcag ccgatcagcc tcggccgcgc cgccggtgtt ctgctggttc tggccggcgt
      781 ccttatggtc cgccggttct aggcctatat cggtcccgat gaccgaggag accctccctg
      841 ccgattcgcc gccgaccccg ctggtcgccg ccgacctgat ccgcgacgag gcgcggcggg
      901 cgccggacaa gccgggcgtc taccgcatgt acggcgagga cgggacctgc ctctatgtgg
      961 gcaaggcgcg gtcgatcaag aagcgcatcc tccagtacgc ccagggccgg ttccacaccc
     1021 agcgcattgg cctgatggtg tccctgaccc gctctatgga actggtcatc accgcctccg
     1081 agaccgaggc cctgctgctg gaatccagct tcatcaaaca gctgaagccg cgcttcaacg
     1141 tggtgctgcg cgacgacaag tcgttcgccg agctgatgat ccgcagggat caccgcgccc
     1201 cgcaggtgcg caagcatcgc ggggcccaca ccacgcccgg cgactatttc ggccccttcg
     1261 cctcgacctg ggcggtgaac cggaccctga acaccctgca gaaggccttt ctgctgcggt
     1321 cctgctcgga cagcgtctac gagacccgca cccggccgtg catgctgcac cagatcaagc
     1381 gctgctcggc gccctgcacc ggcctgatct cgctcgagga ctacggcgtc ctcgtcgacg
     1441 aggcctcggc ctttctgaag ggcaagtcgc gcgccgtcat cgcccgcctg tcggccgaga
     1501 tgagcgccgc cgccgaggcc atggactacg aacaggccgc ccgggtccgc gaccgcatcc
     1561 gcgccctgtc ggcgatctcg atggagaact ccgtcagcgc cgagggcgtg gccgaggccg
     1621 acgtcttcgc cctgttctcg gag
//
LOCUS       DASVVN010000340         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_327001,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000340 DASVVN010000000
VERSION     DASVVN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327001"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VF686_05475"
     CDS             <1..771
                     /locus_tag="VF686_05475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8661296.1"
                     /translation="NVRKGDRVIAVPGWGGMVERLAVPAATVMKIPDAMDFNTAAALV
                     MTYGTSYYALKDRAQLKAGESLLVLGAAGGVGVAAVELGKAMGAHVIAAASTNDKVQF
                     ALEAGADNGLIYPSGKMDKAAQKELSGELKLASGRDGPDVVYDAVGGDYCEPAIRSMD
                     WNGRYLVVGFPAGIPALPLNLTLLKSVSVIGVFWGAAVMRDPAAHAANMADLLKMWTE
                     GKIRPRVSQTFPLERAHEAIKALGDRSVMGKVVVTVEN"
     gene            complement(768..1166)
                     /locus_tag="VF686_05480"
     CDS             complement(768..1166)
                     /locus_tag="VF686_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX8661297.1"
                     /translation="MSAKAKPITPDEALHPARALVPSIQRVNEVRVQKGFWPKIRRSA
                     SRIPFAGQALSVWFAARDPETPTAAKGIMVGALAYFVLPFDAIPDIFAGIGFTDDAAV
                     IAALIATLGANIKRRHKEQADAALERLRQD"
     gene            1221..2003
                     /locus_tag="VF686_05485"
     CDS             1221..2003
                     /locus_tag="VF686_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661298.1"
                     /translation="MKLDGSIAAVVTGGASGLGEGTARAIAATGAKVALFDMNAERGE
                     AVAKEIGGVFCHVDVTDDASVAAGFEKARAAHGQERLTVNCAGIATGQKTVSRKKDTG
                     EIRAHDMAQFERTVRVNLFGTFRVLSQSAAGMVTLEPMADGERGLIVNTASVAAQDGQ
                     IGQAAYSASKGGVYAMTLPVARDLAQEGVRCNTILPGIMWTPMMAGMDQKIQDALAAA
                     IPFPSRLGTPADYASLVLELARNVYINGECIRLDGAIRLAPR"
     gene            2124..2198
                     /locus_tag="VF686_05490"
     tRNA            2124..2198
                     /locus_tag="VF686_05490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2156..2158,aa:Gly,seq:gcc)
BASE COUNT          380 a          778 c          857 g          369 t
ORIGIN      
        1 aacgtccgca agggcgaccg tgtgatcgcc gtgcccggct ggggcggcat ggtggagcgg
       61 ctggcggtcc cggccgccac ggtgatgaag attccggacg ccatggattt caacacggcc
      121 gccgcgctcg tgatgaccta cggcacctcc tactacgcgc tgaaggaccg ggcgcagctg
      181 aaggcgggcg agagcctgct ggtgctgggc gccgcgggcg gcgtcggcgt cgccgcggtc
      241 gagctgggca aggccatggg ggcgcacgtc atcgccgccg cctcgaccaa cgacaaggtg
      301 cagttcgcgc tggaggccgg ggcggacaac ggcctgatct atccgtccgg caagatggac
      361 aaggcggcgc agaaggagct gtcgggcgag ctgaaactgg cctcgggccg cgacgggccg
      421 gacgtggtct atgacgccgt cgggggcgac tattgcgagc cggcgatccg gtcgatggac
      481 tggaacggcc gctatctggt ggtcggtttc ccggccggca ttccggccct gccgctgaac
      541 ctgacgctgc tgaagtcggt gtcggtgatc ggggtgttct ggggcgcggc ggtgatgcgc
      601 gacccggccg cacacgccgc caacatggcc gatctgctga agatgtggac cgagggcaag
      661 atccgtcccc gcgtcagcca gaccttcccg ctggaacgcg cccacgaggc catcaaggcg
      721 ctgggcgacc gctcggtgat gggcaaggtg gtggtgacgg tcgagaacta gtcctgccgc
      781 agccgctcca gcgcggcgtc ggcctgttcc ttgtgccggc gcttgatatt ggccccgagg
      841 gtggcgatca gggcggcgat gaccgcagcg tcgtcggtga agccgatgcc ggcgaagatg
      901 tcgggaatgg cgtcgaaggg caggacgaaa taggccagcg ctccgaccat gatccccttg
      961 gccgccgtcg gcgtctcggg atcgcgggcg gcgaaccaga ccgagagggc ctgtccggcg
     1021 aacgggatgc gcgaggcgga gcgccggatc ttgggccaga accccttctg cacccgcacc
     1081 tcgttgacgc gctggatgga cggcacgagc gcgcgcgccg ggtggagcgc ctcgtcgggg
     1141 gtgatcggtt tggctttggc ggacatggcg gctaaacccc gcggcaatct caaggttcaa
     1201 acaacatagg ggcttcggcc atgaaactcg acggttcgat cgcggcggtg gtgacgggcg
     1261 gggcctcggg tctgggcgag ggcacggcgc gggccatcgc ggccacgggc gcgaaggtcg
     1321 ccctgttcga catgaacgcc gagcgaggcg aggccgtcgc gaaggaaatc ggcggggtct
     1381 tctgccatgt cgacgtcacc gacgacgcct cggtcgccgc cggctttgaa aaggcccgcg
     1441 ccgctcacgg gcaggagcgt ctgaccgtca actgcgccgg catcgccacg ggccagaaga
     1501 ccgtcagccg caagaaggac acgggcgaga tccgcgccca cgacatggcc cagttcgaac
     1561 gcactgttcg ggtcaatctg ttcggcacct tccgcgtcct gtcgcagtcg gcggcgggga
     1621 tggtgacgct ggagcccatg gccgacggcg agcgcggcct gatcgtcaac accgcctcgg
     1681 tggcggccca ggacggtcag atcggccagg ccgcctattc cgcgtccaag ggaggcgtct
     1741 acgccatgac cctgccggtc gcccgcgacc tcgcgcagga gggcgtccgc tgcaacacca
     1801 tcctgcccgg catcatgtgg acgccgatga tggccggcat ggaccagaag atccaggacg
     1861 cactggccgc agccattccc ttccccagcc gtctcggcac gccggcggac tacgcctcgc
     1921 tggtgctgga gctggcccgc aacgtctaca tcaacggcga atgcatccgg ctggacgggg
     1981 ctattcgtct cgccccgcgc tgacgcgcgg ccagggcgct atcgctcggc ttgcgaagcc
     2041 tcgctgcttg agcgctagga aatgtgtgtt ggggacttgc gaggggccgg agccctaggt
     2101 atacgcccgc ctccgactga agtgcgggcg tagctcagtg gtagagcaca accttgccaa
     2161 ggttggggtc gagagttcga atctcttcgc ccgctccaat ttctcgcaaa ctcagacttc
     2221 agcctagccc cgcaggggcc ctgccgcgtg caggctgcgg cgtttgccga accgtgctat
     2281 cctcccgttc cgatgctgcc gagcgccgcg ccatcacgga aacgacgggg cccggcgccg
     2341 acgggtaagg gctttcctgt cctcgtccgg cttcaacccc atca
//
LOCUS       DASVVN010000341         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_144798,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000341 DASVVN010000000
VERSION     DASVVN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144798"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF686_05495"
     CDS             complement(<1..185)
                     /locus_tag="VF686_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8661299.1"
                     /translation="MSRPMNIVVLGLSLSSSWGNGHAVTWRALLKALAARGHRILFLE
                     RETPWYAAHRDLESPDW"
     gene            complement(182..1273)
                     /locus_tag="VF686_05500"
     CDS             complement(182..1273)
                     /locus_tag="VF686_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8661300.1"
                     /translation="MKIAFYGSSLLSAYWNGAATYYRGMLRELAARGWEVTFYEPDAF
                     DRQQHRDIDPPDWARVVVWSNTPDACREVVASAADADVVVKASGVGVFDNELLSVFDH
                     ARPDALTVYWDVDAPATLAELRDQPGHPLRRALPDIDMVLTYGGGPPVVSAFGALGAR
                     LCRPIYNALDPTTHHRVEPQARFAADLSLLANRLPDREARIERFFIEPAASLPGRSFL
                     LGGNGWSDKPLPANVRTLGHVGTADHNAFNCSSLAVLNVSRDSMAENGFSPATRVFEA
                     AGAGACLITDHWEGIDQFLKPDEEVLVARDGREVADHLAALTPGRARAIGEAARRRVL
                     AGHTYARRADEADALFRHALAAHRERSAA"
     gene            complement(1273..2388)
                     /locus_tag="VF686_05505"
     CDS             complement(1273..2388)
                     /locus_tag="VF686_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8661301.1"
                     /translation="MKVVYFTHSLESCWNHGNAHFLRGVLRDLIARGNEVAVHEPRDA
                     WSRANLIADHGEAGLEPYRRAYPELASRRFDEPKLEQALDGADLVIVHEWNQPALIAA
                     IGRARKRGARFTLLFHDTHHRAVSEPDGIKAFDLSGYDGVLAFGETLAEVYRRWGWGD
                     RVFVWHEAADVRLFHPPAQETPREGLIWIGNWGDGERTKELETFLFAPARDAALGLDI
                     HGVRYPPDALATLRRYGVRYHGWLANAAAPAAFARHMATVHVPRRFYVRQLPGIPTIR
                     VFEALACGIPLVSAPWEDSEGLFTIGEDFLMARSGPEMTQTLRRLKADPELRAALAER
                     GLATIRARHTCTHRVDELLAIVARLQSASMTATMETA"
BASE COUNT          379 a          845 c          919 g          368 t
ORIGIN      
        1 caccagtcgg gggactcaag gtcgcggtgg gccgcgtacc aaggggtttc tcgctcgaga
       61 aacaggatgc ggtgaccccg cgccgccagc gccttcagca gggcccgcca tgtgaccgca
      121 tggccgttgc cccatgagga cgacaggctc agccccagca cgacgatatt catgggccgg
      181 ctcatgcggc gctcctttcg cggtgggcgg ccagggcgtg gcggaacagg gcgtcggcct
      241 cgtcggcgcg ccgggcatag gtgtgcccgg ccagcacccg gcggcgggcc gcttcgccga
      301 tggcgcgggc ccggccgggc gtaagggcgg cgagatggtc cgcgacctcc cgcccgtcac
      361 gggcgaccag cacctcctcg tcgggtttga ggaactgatc gatgccttcc cagtggtcgg
      421 tgatcagaca ggcgccggcg ccggctgcct cgaacacgcg cgtggccggg gagaagccgt
      481 tctcggccat gctgtcgcgc gacacgttga ggaccgccag cgaggagcaa ttgaaggcgt
      541 tgtggtccgc cgtgccgaca tggccgaggg tgcggacgtt ggccggcagc ggcttgtcgc
      601 tccagccatt gccgccgagc aggaaactgc gccccggcag gctcgcagcc ggctcgatga
      661 agaaacgctc gatccgcgcc tcccggtccg gcaggcggtt ggccagcagc gacaggtccg
      721 ctgcgaaccg ggcctgcggc tcgacgcggt ggtgggtggt cgggtccagg gcgttgtaga
      781 tcggccggca cagcctcgcg cccagcgccc cgaacgcgga tacgacgggc gggccgccgc
      841 cataggtgag caccatgtcg atgtccggca gcgcgcgccg cagcggatgg cccggctgat
      901 cccgcagctc cgccagggtc gccggcgcgt cgacatccca gtagaccgtc agggcgtcgg
      961 gccgggcgtg gtcgaagacc gacagcagtt cattgtcgaa aaccccgacg cccgaggcct
     1021 tgaccacgac atcggcgtcg gcggcgctgg cgaccacttc gcggcaggcg tcgggcgtat
     1081 tgctccagac caccacccgc gcccagtccg gcggatcgat gtcgcggtgc tgctggcgat
     1141 cgaaggcgtc gggttcgtag aaggtcacct cccagccgcg ggcggccagt tctcgcagca
     1201 tgccgcggta ataggtcgcc gcgccgttcc agtaggctga cagcaggctg gacccgtaga
     1261 aggcgatctt catcaggcgg tctccatcgt ggcggtcatg gacgcggatt gtaggcgcgc
     1321 gacgatagcc agcagctcgt cgacgcggtg agtgcaggtg tgcctggcgc ggatcgtggc
     1381 gaggccgcgc tcggccagcg ccgcgcgcag ctcgggatcg gctttcagcc tgcgaagtgt
     1441 ttgggtcatc tccgggcccg atcgggccat gaggaaatcc tcgccgatgg tgaacaggcc
     1501 ttcgctgtcc tcccagggcg cgctgaccaa agggatgccg caggccagcg cctcgaacac
     1561 gcggatcgtg gggatgcccg gcagctggcg cacatagaag cggcgaggca catgcacggt
     1621 ggccatgtgg cgcgcgaagg cggcgggggc ggcggcgttg gccagccagc cgtgatagcg
     1681 gacgccatag cgccttagcg tggccagcgc atcgggcgga tagcggacgc cgtggatgtc
     1741 gagccccagt gcggcgtcgc gggcaggggc gaacaggaag gtttcgagtt ccttcgtccg
     1801 ctctccgtcg ccccagttgc cgatccagat cagcccttcc cgcggcgtct cctgcgcggg
     1861 cggatggaac agccgcacgt cggcggcctc atgccagacg aaaacccggt cgccccagcc
     1921 ccagcggcga tagacctcgg ccagtgtctc gccgaaggcc agaacgccat catagccgga
     1981 gagatcgaac gccttgatcc cgtccggctc gctgacggcc cggtggtggg tgtcgtggaa
     2041 cagcagggtg aaccgcgcgc cccgcttgcg ggcccggccg atggcggcga tcagggccgg
     2101 ctggttccac tcgtggacga tgaccagatc ggcgccgtcc agcgcctgct ccagtttcgg
     2161 ctcatcgaaa cgccggctgg ccagttccgg ataggcgcgg cggtagggct ccagccccgc
     2221 ctcgccgtgg tctgcgatca ggttggcgcg gctccaggcg tcgcgcggtt catggaccgc
     2281 gacctcgttc ccccgggcga tcaggtcgcg caggacgccg cgcaggaaat gcgcattgcc
     2341 gtggttccag cacgactcca gcgaatgggt gaaatagacg accttcacgc cgccacttcc
     2401 gggaagggcg cgcgcgcggc gagggcttcg ccgtagacgg cgagcgtgcc ctcgaccatg
     2461 gcgtcgacgg tgaagcgggc gctgcgttcg gccgcgcatc gtcccagttc g
//
LOCUS       DASVVN010000342         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_223888,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000342 DASVVN010000000
VERSION     DASVVN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223888"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VF686_05510"
     CDS             <1..750
                     /locus_tag="VF686_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8661302.1"
                     /translation="MGAIMNGIALHGGTRVYGGTFLTFSDYMRGAVRLAALMQLPVTY
                     VWTHDSIGLGEDGPTHQPVEHLAALRAIPNLLVFRPADTVEAMECWQLALEHKAAPSA
                     MALSRQKTAAVRTVAASENLSAKGAYELRAATGAAKATLFATGTEVPLALAAAEQLEA
                     EGTPTRVVSVPCFELFFQQPKAYQDGVIGRGTVRVAVEAAIQQGWERFIGEDGGFVGM
                     TGFGASAPAEVLYAKFGITTEAVVAAVKARL"
     gene            750..1532
                     /locus_tag="VF686_05515"
     CDS             750..1532
                     /locus_tag="VF686_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661303.1"
                     /translation="MRIAAGLACLGLLTGCGFTEPSGAAVHSASPLRWGPDIADDFHA
                     LPRVAADTRQARAINGFLDSLDVRDREERSTCLAESPENVEWGRFIEAPMTGPRFVSL
                     IVTIGYYCGGAHPYWTQTHYTFDLHTGQQVDWSVLLPDDMSRPLQEPRYSWPALLASP
                     SLTAWFAERALAGMDERGRDDCGYVYGLEGRKYWSLAVWPDAKEGGLGLQPVGLAHAE
                     TGCLVSATIPLAELRRRGAAPELTDAIEAAQAAESWHGPRPE"
     gene            1532..1924
                     /locus_tag="VF686_05520"
     CDS             1532..1924
                     /locus_tag="VF686_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8661304.1"
                     /translation="MNLSAVSLLVRDYDEAIAFFVGKLGFELSEDTDMGGGKRWVRVT
                     PKDGQTSLLLARATTDAQRTAVGKQAGDRVWLFLETDDLIRDHAAWTAAGVEFRETPR
                     QESYGKVVVFRDLYGNAWDLIEPARETR"
     gene            1954..>2417
                     /locus_tag="VF686_05525"
     CDS             1954..>2417
                     /locus_tag="VF686_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8661305.1"
                     /translation="MSGALLTAAPVTAQSPTPVVIGQSYALPSAVMGAARELNVWLPP
                     GYEGGAARYPVLYVLDGGQGQDFHHISGLAQLGTVNGTTRDVIVVGIASVDRRNELAL
                     ATGNAELIARYPTQGHSDRFRRFIAEEVQPFIDERFRTNGETALMGESLAGL"
BASE COUNT          381 a          814 c          827 g          395 t
ORIGIN      
        1 atgggcgcga tcatgaacgg gatcgccctg cacggcggca cccgcgtcta cggcgggacg
       61 ttcctcacct tctccgacta catgcgcggc gccgtccgcc tggccgcgct gatgcagctg
      121 ccggtcacct acgtctggac acacgactcg atcggcctcg gtgaggacgg tcccacccac
      181 cagccggtcg agcacctcgc cgccctgcgc gccattccca acctgctggt cttccgcccc
      241 gccgacacgg tcgaggccat ggagtgctgg cagctggcgc tggagcacaa ggccgcgccg
      301 tcggccatgg ccctgtcgcg ccagaagacc gccgccgtac gcacggtcgc tgcgtcggag
      361 aacctgtcag ccaagggcgc ctatgagctg cgcgccgcca ccggcgcggc gaaggcgacc
      421 ctgttcgcga ccggcaccga ggttccgctg gcgctggccg ccgccgagca gctggaggcc
      481 gagggcacgc ccacgcgggt ggtttcggtc ccctgtttcg aactcttctt ccagcagccg
      541 aaggcgtatc aggacggcgt catcggccgc ggcaccgtcc gcgtcgccgt cgaggccgcc
      601 atccagcagg gctgggagcg gttcatcggc gaggacggcg gcttcgtcgg catgaccggc
      661 ttcggcgcgt cggcgccggc cgaggttctg tacgcgaagt tcgggatcac caccgaggcc
      721 gtggtcgcgg cggtcaaggc gcggctctga tgcggattgc ggccggtctc gcatgccttg
      781 ggctgctgac cggttgcggt ttcaccgaac cgtcgggagc agcggttcac agcgcgtcac
      841 ccctgcgttg ggggccggat attgctgacg acttccacgc cttgccgcgg gtcgctgcgg
      901 acacgcggca agcgcgcgcg atcaacggtt ttctggacag tctcgacgtc cgcgatcgag
      961 aggaacgctc cacctgcctt gcggagtccc ctgaaaatgt cgagtgggga cgcttcatcg
     1021 aagcaccgat gaccggtccg cgtttcgtga gcctgatcgt caccatcggc tactattgcg
     1081 gcggcgccca tccgtattgg acccagaccc actacacgtt cgaccttcac accgggcaac
     1141 aggtggattg gtcggtgctg cttcccgacg acatgagccg cccactccag gagccgcgat
     1201 acagctggcc agcactgctg gcctcgcctt cccttacggc gtggttcgcc gaacgcgccc
     1261 tcgccggcat ggacgagcgg gggcgggacg attgcggcta cgtctacggc ctggagggtc
     1321 gcaaatattg gtcattagct gtctggccag acgcgaagga aggcggattg ggtttgcagc
     1381 ctgtcggact ggcgcatgca gaaacgggct gccttgtttc ggcgaccatc ccattggccg
     1441 agctcaggcg acgcggagca gccccggaac tgaccgacgc catcgaggcc gcgcaggccg
     1501 ccgaaagctg gcacggaccc cggcccgaat aatgaacctc tccgccgtat ccctgctggt
     1561 ccgcgattac gacgaggcca tcgccttctt cgtcggcaag ctgggtttcg agctcagcga
     1621 ggacacggac atgggcggcg gcaagcggtg ggtgcgggtc acgcccaagg acgggcagac
     1681 ctccctgctg ctggcccggg ccacgacgga cgcccagcgc acggcagtcg gcaaacaggc
     1741 gggcgaccgc gtctggctgt ttctggagac ggacgatctg atccgcgatc acgccgcatg
     1801 gacagccgcg ggcgtggaat ttcgagagac accgcggcag gaatcctatg ggaaggtagt
     1861 cgtattccgg gacctttacg gcaacgcctg ggacctgatc gaacctgccc gggagacccg
     1921 atgatgttgc gaacagtgat gagggccctg ctgatgtccg gcgcgcttct gaccgcagcc
     1981 cccgtcacgg cccagtcgcc cacgccggtg gtcattggcc aatcgtacgc gctgccttcg
     2041 gcagtgatgg gcgcggcgcg cgagctcaac gtctggctgc cgccggggta tgaaggcggc
     2101 gcggcgcggt atccggtgct gtatgtgctg gacggcggtc aggggcagga cttccaccac
     2161 atctccggcc tggcccagtt ggggacggtg aacggaacga cgcgcgacgt gatcgtggtc
     2221 gggatcgcct cggtggaccg ccgcaacgag ctggcgctgg ccacgggcaa cgccgaactg
     2281 atcgcccgct acccgacgca gggccactcc gaccggtttc gccggttcat cgcagaggag
     2341 gtccagccgt tcatcgacga acgcttccgc accaatggcg agacggcgct tatgggcgag
     2401 tcgctggccg ggctgtt
//
LOCUS       DASVVN010000343         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_383283,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000343 DASVVN010000000
VERSION     DASVVN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383283"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF686_05530"
     CDS             <1..591
                     /locus_tag="VF686_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HEX8661306.1"
                     /translation="ILDRSGQRPLLRFDRRDETWVLRPTAAPRGDVIYRNDAGAQLLR
                     VTPGGGMTVYTPRAPGGSPASLAGPAASLTPPTLGPAQMFQLMTRRSAMLSQALGRLI
                     EINVDTDGAAESLTVEALILSTDAALRIARSPSARSSLDRLRSITIVDGPRSSVTYAR
                     GDLRIVVSPTQGVAGRPSSARVIDAFLTPAAAPRRR"
     gene            complement(592..1320)
                     /gene="gloB"
                     /locus_tag="VF686_05535"
     CDS             complement(592..1320)
                     /gene="gloB"
                     /locus_tag="VF686_05535"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX8661307.1"
                     /translation="MTLDVRIFPCLNDNYGLLVRDAASGVVAAIDTPDAAAILADLER
                     SGWGRLDLVLNTHWHPDHTQGNALLKDETGCEIVGPEEVRKAAPIDRVVAGGDTVMLG
                     ETVLEVTHTPGHTLGHIVYRSAADAQAFVGDVLFNLGCGRLIEGAPEQMHASLQTLAA
                     WPDETVVWCAHEYTAANARFALGLDDRPEMRARADEIFAMRERGEATVPTTIGAEKAF
                     NPFLRAKDAAEFTVRRAAKDVFAG"
     gene            1366..2115
                     /locus_tag="VF686_05540"
     CDS             1366..2115
                     /locus_tag="VF686_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8661308.1"
                     /translation="MRRSIEDLRIFYGEPAGALARRLLARRLEDAWGEAANSDVLGLG
                     YATPWLDAFVGARRVVAAMPGGQGVELWPSVGKNRTLLVDDRRLPFAAGSFDRILLVH
                     ALEEADDAQALMAEAVRALSPAGRIILVAAARGGLWARAETTPFGHGRPFTRGQLERL
                     VRAAGLEPTAWSQTLYVPPWGPLLPLADGFEQVGKRIMPGAAGVILLEATRREYARIR
                     PGGAVATVPVMTPALQPQPASRSGRETLAKN"
     gene            2164..2667
                     /locus_tag="VF686_05545"
     CDS             2164..2667
                     /locus_tag="VF686_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8661309.1"
                     /translation="MQRLILMRHARAEKSSASGHDRDRPLSDTGRADAAAMGRALAAR
                     GMKPDLAFVSPSTRTHQTWDLLHDAFGDVQVREPEPLYNGGSDTLRRYIVGAEDEAGC
                     LMVVGHNPGVHVLAVEYLIESAASPSVLDRMSGGFPPGAAAIFIVDIAGRCTFEGFLQ
                     PRDLAAE"
     gene            2664..3020
                     /locus_tag="VF686_05550"
     CDS             2664..3020
                     /locus_tag="VF686_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX8661310.1"
                     /translation="MTDVAYKLVYRAEWAAARAGGAYAGSVVDLLDGYIHLSGPDQLA
                     ETARRHYAGRADLMLVTVNLRPLGEDLKWEESRGGVLFPHLYAPLPIHAAIGERPLSV
                     DADGVMRFDDGAVGWP"
     gene            3011..4048
                     /locus_tag="VF686_05555"
     CDS             3011..4048
                     /locus_tag="VF686_05555"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX8661311.1"
                     /translation="MALNDLGAALLRQLDPEVAHGLAIHALQTVPLPKAPADDPVLQT
                     TIAGLDLPNPVGLAAGLDKNGEALAGLARLGFGFVECGSVTPVAQAGNPKPRLFRLSE
                     DRAIINRMGFNNLGLEAFAERLADRPRTVVVGANLGANKDTEDKAADYVAGLTRLRGL
                     SDYVTVNVSSPNTPGLRALQGRAALDDLLGRLNEARTGDPTPVFLKVAPDLTGDEIAL
                     IVEASLDHRIDALIVSNTTLDRPASLRSRDRVEAGGLSGAPLKSRAMQALRAAADASA
                     GRLPLIAVGGIDSGAEAYARIRAGAGAVQLYSALIYEGPGLVGRIKRDLAARLRADGF
                     STVSEAARSAR"
     gene            4113..>4579
                     /locus_tag="VF686_05560"
     CDS             4113..>4579
                     /locus_tag="VF686_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661312.1"
                     /translation="MAIATVATQAHGAPLEGGWAIAIRQRRKALLQRLGMAAATAMIF
                     SPLLGWPLSVAWVLGYFTIQALDVWVFSPINSGRAERLTGFRNLAGWLMLIGNAGYFG
                     SLSIPMWLLGGPMGGICAAMLLSAGCIYSVINAPRSTSVMLMTASVQFLYLAAT"
BASE COUNT          631 a         1639 c         1603 g          706 t
ORIGIN      
        1 atcctcgacc gttcaggcca gcggcccctg ctgcgtttcg atcggcgcga cgagacctgg
       61 gtgctgcgcc cgaccgcggc cccgcgaggc gacgtcatct atcgcaatga cgccggcgcc
      121 cagctgctgc gggtcacgcc cggcggcggc atgaccgtct atacgccccg cgcgcccggc
      181 ggatcgcccg cctcgctggc gggcccggcc gccagtctga cgccgccgac gctgggcccg
      241 gcccagatgt tccagctgat gacccgccgc agcgcgatgc tgagccaggc attgggtcgg
      301 ctgatagaaa tcaacgtcga cacggacggc gccgccgaga gcctgaccgt ggaggccctg
      361 atcctgtcta cggacgccgc cctgcgcatc gcgcgctcgc cctccgcccg ttcttcgctg
      421 gaccggctcc gcagcatcac catcgtcgac ggcccccgct catcggtgac ctacgcccgc
      481 ggcgacctgc gcatcgtggt gtcgcccaca cagggcgtcg cgggccggcc ctcgtcggcc
      541 cgagtcatcg acgccttcct gacccccgcg gccgcgccgc gcaggcggta gtcagccggc
      601 gaagacgtcc ttggcggccc gccggacggt gaactcggca gcatccttcg cccggaggaa
      661 tgggttgaag gccttctccg ccccgatggt cgtcggcacc gtcgcctccc cacgctcccg
      721 catggcgaag atctcgtcgg cccgcgcccg catttcgggc cggtcatcga ggccgagggc
      781 gaagcgggcg ttggcggcgg tgtattcgtg ggcgcaccag accaccgtct cgtccggcca
      841 ggccgccagc gtctgcaggc tggcgtgcat ctgctccggc gcgccctcga tcagccggcc
      901 gcagccgagg ttgaacagca cgtccccgac gaaggcctgg gcgtccgctg ccgagcggta
      961 gacgatgtga cccagggtgt ggccgggcgt gtgggttacc tccagcacgg tctcgcccag
     1021 catcaccgtg tcgccgccgg ccacgactcg gtcgatgggc gcggccttgc gcacctcttc
     1081 ggggccgacg atttcgcaac ccgtctcatc cttcagcagg gcgttgccct gggtgtgatc
     1141 ggggtgccag tgggtgttca ggacaagatc cagccgcccc cagcccgagc gttccagatc
     1201 ggccagaatg gcggcggcgt ccggcgtgtc gatcgccgcg accacgcccg aggccgcgtc
     1261 gcggaccagc aggccatagt tgtcgttcag acaggggaag atgcggacgt cgagggtcat
     1321 gcgaccatcc tagcagcccg ccccgacccg gcctatgttc ctcccatgcg gcgcagtatc
     1381 gaggaccttc gcatcttcta cggcgagccg gccggggccc ttgcgcggcg tctgctggcg
     1441 cggcgtctgg aggacgcctg gggcgaggcc gccaacagcg atgtcctggg tctgggctac
     1501 gccacgccgt ggctggacgc cttcgtcggc gcgcgccgcg tcgtcgcagc catgccgggc
     1561 ggacaaggag tcgagctgtg gccctcggtc ggcaagaacc ggacgctgct ggtcgacgac
     1621 cgccgcctgc ccttcgccgc cggatcgttc gaccgcatcc tgctggtcca cgccctcgaa
     1681 gaagccgacg acgcccaggc cctgatggcc gaggccgtgc gcgccctgtc gcccgcggga
     1741 cggatcatac tggttgcggc ggcgcgcggc gggctgtggg cgcgggccga gaccacgccg
     1801 ttcggtcacg gcaggccctt cacccgcggc cagctcgagc ggctggtgcg cgccgccgga
     1861 ctggagccga cggcatggtc ccagacgctg tatgtgccgc cgtggggacc actgctgccg
     1921 ttggcggacg ggttcgaaca ggtcggcaaa cggatcatgc cgggcgcggc gggggtcatc
     1981 ctgctggagg cgacgcgccg cgaatacgcc cgcatcaggc cgggcggagc cgtggcgacc
     2041 gtgccggtga tgaccccggc cctgcagccc cagcccgcga gccggtcggg ccgcgagacg
     2101 ctcgccaaaa actgaccggc ggcgatctgg cgggaatccg cgaacgccct taaagccacg
     2161 gccatgcagc gactgatcct catgcgccac gccagggcgg agaagagttc cgcctcgggc
     2221 catgaccgcg accgcccgct gtcggacacg ggacgggccg acgccgccgc catgggccgg
     2281 gccctggccg cgcggggcat gaagccggat ctggccttcg tctcaccctc gacccggacg
     2341 catcagacct gggacctgct gcacgacgct ttcggcgatg tgcaggtgcg cgagcccgag
     2401 ccgctgtaca atggcggatc cgacacactg cgccgctaca tcgtgggggc cgaggacgag
     2461 gccggctgcc tgatggtggt gggccacaat ccgggtgtcc atgtgctggc cgtggagtat
     2521 ctgatcgaaa gcgccgcctc gccgtcggtg ctggaccgga tgagcggcgg cttcccgccg
     2581 ggcgcggcgg ccatctttat cgtcgacatc gccgggcgct gcaccttcga gggcttcctg
     2641 cagccccgcg acctcgccgc cgaatgaccg acgtcgcgta caagctcgtc taccgggccg
     2701 aatgggccgc cgctcgcgcc gggggcgcct atgccgggtc ggtagtcgat ctgctggacg
     2761 gctacatcca cctgtcgggc cccgaccagc tggccgagac ggcgcggcgg cactatgctg
     2821 gccgcgcgga tctgatgctg gtgaccgtga acctgcggcc gcttggcgag gacctgaagt
     2881 gggaggagtc gcggggcggc gtactgtttc cgcatctcta cgcgcccttg ccgatccatg
     2941 ccgcgatcgg ggaacggccg ctgtcggtgg acgccgacgg cgtgatgcgt ttcgacgacg
     3001 gagcggtcgg atggccctga acgacctcgg cgccgccttg ctgcgccaac tggaccccga
     3061 ggtcgcccac ggtctggcca tccacgccct gcagaccgtt cctctgccaa aggcgcccgc
     3121 ggacgacccc gttctgcaaa ccacgatcgc gggactggat ctgccgaacc ccgtcggtct
     3181 ggcggccggg ctggacaaga acggcgaggc tctggcgggt ttggcgcggc tcggcttcgg
     3241 cttcgtggaa tgcggctcgg tcacgcccgt cgcgcaagcc ggcaacccga agccgcgtct
     3301 cttccggctg tcggaagacc gcgccatcat caaccggatg gggttcaaca atctgggcct
     3361 cgaggccttc gccgaacgct tggcggaccg gccccggacg gtcgtggtcg gggccaatct
     3421 gggggccaac aaggacacgg aggacaaggc cgccgactat gtggcggggc tgacgcgtct
     3481 gcgcgggctc agcgactatg tcaccgtcaa tgtgtcgtct cccaacacgc cggggcttcg
     3541 cgccctgcag gggcgggcgg cgctggacga tttgctgggg cggctgaacg aggcccgcac
     3601 cggcgatccg accccggtct tcctcaaggt cgcacccgat ctgaccgggg acgaaatcgc
     3661 cctgatcgtc gaggccagcc tggatcaccg gatcgacgcc ctgatcgtct ccaacaccac
     3721 cctggaccgg cccgccagcc tgcgctcgcg ggaccgcgtt gaagcgggag gcctgtccgg
     3781 cgcgccgctg aagtcccgcg ccatgcaggc gttgcgcgcc gccgcggacg cctcggcggg
     3841 ccgcctgccc ctgatcgcgg tcgggggaat cgattcgggc gccgaggcct atgcccgcat
     3901 ccgggccggg gcgggggccg tccagctgta ttcggccttg atatacgagg gcccggggct
     3961 ggtcgggcgg atcaagcgcg atctggccgc ccggttgcgc gcggacggct tctccacagt
     4021 gtccgaggcc gcccgatccg cccgttgacg gagcgttagg gcaaggcggg catagtgcgc
     4081 gcgaggcgaa aacgccttgc ccggggaacc gcatggcgat cgcgaccgtc gcaacgcaag
     4141 ctcacggcgc ccctctcgag ggcggctggg cgattgcaat tcgccagcga cgcaaggcgt
     4201 tgctgcaacg gctcggcatg gccgcagcca ccgccatgat tttcagcccc ctgctgggct
     4261 ggccgctgag cgtcgcctgg gtgctgggct atttcaccat ccaggccctg gacgtctggg
     4321 tcttttcacc gatcaacagc ggccgcgccg agcgtctgac cggcttccgc aatctcgcgg
     4381 gctggctgat gctgatcggc aacgcgggct atttcggcag tctgtccatc ccgatgtggc
     4441 ttctgggcgg tcccatgggc ggcatctgcg ccgccatgct gctgtccgcg ggctgcatct
     4501 attcggtgat caatgcgccg cggtcgacgt ccgtcatgct gatgacggcc tcggtccaat
     4561 tcctctatct ggccgccac
//
LOCUS       DASVVN010000344         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_117816,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000344 DASVVN010000000
VERSION     DASVVN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117816"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1505
                     /locus_tag="VF686_05565"
     CDS             135..1505
                     /locus_tag="VF686_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8661313.1"
                     /translation="MRKLLLIGAAAVAMVGVGAATAAGQTPRNEAFRMLGLFGDVLGI
                     VEQAYVVPVDDKKLIEAALQGMMTALDPHSNYLPPDGYGDLQERTSGAYSGVGLTIQS
                     EAGLVKVISPMDGGPAARAGVNAGDVISSIDGQNASGLTVSQVSEKLRGAVGTSVKVT
                     FLRDGEEPKEVVLTREVIKIESVTGRVEGEFGYLRVSTFNENTGRELSETIAKLKAEN
                     PNIKGYVLDLRNNGGGLLDAAISVSDAFLERGEIVSQRGRKADQIQRYAARPGDQTGG
                     LPLVVLINYGSASASEIVAGALKDHQRATLVGLTSFGKGSVQSVIPLRGGADGALSIT
                     TARYYTPSGASIQKIGIEPDLEVSRSAAEAKIVSRSNFIYSEAAYATALDSSIGAERK
                     GPHIPREAPGEAYDEDKDYQLERAFDVLRAGGNLAQLAAAPEGVVVSEPGATLAAVQP
                     DPAPEN"
     gene            1623..2822
                     /locus_tag="VF686_05570"
     CDS             1623..2822
                     /locus_tag="VF686_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX8661314.1"
                     /translation="MFAKRQSVLAATAGIPPVRRSMRENLAPVVRFLSKPPVAVAGAG
                     LMLMGAAALFITVLGDPRAGAPSARAALKREAPAAAPVPTGLEAFSIGAYGSWQDMAA
                     EGGAIDPALAGEAVITLPNGATVSGSGAPVTAPRVAASPLPAAPIAGLSQPGPNGPLP
                     AIASDGRVPAQAYARPFRSNGKPRVALIVGGLGLNAVTTRAAIERLPADVTLSFVPYA
                     EGLQTWIDLARAQGHEVMLEMPMEPTGYPDNDPGPYTLLASGSPADLQSKMNWLLGRA
                     TGYFGVTNYMGDRFAASDAGMNAFLGVLRQRGVAFLDDGSMSRRPGAWARASANRVID
                     EQQTPAAIVGQLNALEALAKGRGQAMGTGFSYPVTVEAVARWTAELDARGLQLAPASA
                     MTQRPGR"
     gene            2954..>3032
                     /locus_tag="VF686_05575"
     CDS             2954..>3032
                     /locus_tag="VF686_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX8661315.1"
                     /translation="MTDLSRYRPNVGVVLFHPDGRVWYGR"
BASE COUNT          462 a         1104 c         1010 g          456 t
ORIGIN      
        1 ttgcgttaag ggcgaaaccg cttcccaagc cgcgatgacg ctgattttat cgacaaaccg
       61 ccgctcggcc acgatttcgt cgcctcgcgg ttcgtatctg atccaagact tcgcctgacc
      121 ctgaaagaaa ccccatgcgt aaactgcttc tgattggcgc cgccgccgtg gccatggtcg
      181 gcgtaggcgc cgccaccgcg gccggccaga cgccgcgcaa cgaggccttc cgcatgctgg
      241 ggctgttcgg cgacgtgctg ggcatcgtcg aacaggccta tgtcgtgccg gtcgacgaca
      301 agaagctgat cgaggcggcg ctgcagggca tgatgacggc cctggatccc cactccaact
      361 atctgccgcc cgacggctat ggcgatctgc aggagcgcac ctcgggcgca tactcgggcg
      421 tcggcctgac tatccagtcc gaggccgggc tggtgaaggt catctcgccc atggacggcg
      481 ggccggcggc gcgcgcgggc gtcaacgccg gcgacgtcat ctcctccatc gacggccaga
      541 acgcctcggg cctgacggtc agccaggtgt ccgaaaagct gcgcggcgcc gtcggcacct
      601 cggtcaaggt caccttcctg cgcgacgggg aggagcccaa ggaggtcgtc ctgacccgcg
      661 aggtcatcaa gatcgaatcc gtcaccggcc gcgtcgaggg cgagttcggc tatctgcgcg
      721 tctcgacctt caacgagaac accggccgcg agctgagcga gacgatcgcg aaactgaaag
      781 ccgagaaccc gaacatcaag ggctatgtcc tcgacctgcg caacaacggc ggcggtctgc
      841 tggatgcggc gatctcggtg tccgacgcct tcctcgagcg gggcgagatc gtcagccagc
      901 gcgggcgcaa ggcggatcag atccagcgtt acgccgcccg cccgggcgac cagaccggcg
      961 gcctgccgct ggtggtgctg atcaactatg gatcggcctc tgcctcggag atcgtggccg
     1021 gcgcgctgaa ggaccaccag cgggccacgc tggtcggcct gaccagcttc ggcaaggggt
     1081 cggtgcagag cgtcatcccg ctgcgcggcg gggccgacgg cgccctgtcg atcaccacgg
     1141 cgcgctacta caccccgtcc ggcgcctcga tccagaagat cggcatcgaa ccggatctgg
     1201 aggtgtcgcg ttcggcggcc gaggcgaaga tcgtgtcgcg gtccaacttc atctattccg
     1261 aggccgccta cgccaccgcc ctggactcgt cgatcggcgc cgagcgcaag gggccgcaca
     1321 tcccccgcga agccccgggc gaggcctatg acgaggacaa ggactaccag ctggagcggg
     1381 ccttcgacgt gctgcgcgcc gggggcaatc tggcccagct ggccgccgcg ccggagggcg
     1441 tggtggtgtc cgagccgggc gcgaccctgg ccgccgtcca gccggatccc gcgccggaaa
     1501 actgaggcgg gtgcgagcgt tagacgccta acgccgcgtt aggctttctt aaccgacgcc
     1561 cgccactgtg gccgctgaag gagtccgctt cgcggacccc gcatcaagcg tccagtcctg
     1621 ccatgttcgc caagcgccag tctgttctag ccgccaccgc cgggattcct ccggtgcgcc
     1681 gctcgatgcg cgaaaacctc gcgcccgtgg tccgttttct cagcaagccg cccgtcgccg
     1741 tcgccggggc cggactcatg ctgatgggcg ccgccgccct gttcatcacc gtgctgggcg
     1801 atccccgcgc cggagccccg tcggcccgcg cggcgctgaa gcgcgaagcc cccgccgccg
     1861 cgcctgtccc gaccggtctg gaagccttct ccataggcgc ctacggctcg tggcaggata
     1921 tggcggccga aggcggcgcc atcgatccgg cgctcgccgg ggaggcggtc atcaccctgc
     1981 ccaacggcgc gacggtttcg ggtagcggcg ctccggtcac cgcgccgcgc gtcgcggcct
     2041 cgcccctgcc cgccgccccc atcgccggac tgagccagcc gggcccgaac ggccccctgc
     2101 cggccatcgc cagcgacggc cgcgtccccg cccaggccta tgcccggccg ttccgctcga
     2161 acggcaagcc gcgcgtggcg ctgatcgtcg gcggtctggg cctgaacgcc gtgaccaccc
     2221 gcgccgccat cgaacgcctg cccgccgacg tgacgctgag cttcgtcccc tatgccgagg
     2281 gtctgcagac ctggatcgat ctggcccggg cgcagggtca cgaggtgatg ctggaaatgc
     2341 cgatggagcc caccggctat cccgacaacg atcccggccc ctacaccctg ctggcctcgg
     2401 gctcgcccgc cgatctgcag tccaagatga actggctgct gggccgggcc accggctatt
     2461 tcggcgtcac caactatatg ggcgaccggt tcgcggcctc ggatgcgggt atgaacgcct
     2521 tcctcggggt cctgcgccag cgcggcgtgg ccttcctcga cgacggttcg atgagccgcc
     2581 ggccgggcgc ctgggcccgc gccagcgcca accgggtcat cgacgaacaa cagacccccg
     2641 ccgccatcgt cggccagctg aacgcgctcg aggccctcgc caagggccgc ggtcaggcca
     2701 tgggcacggg cttcagctat ccggtcacgg tcgaggccgt ggcgcgctgg acggccgagc
     2761 tcgacgcacg tggcctgcag ctggctccgg cgagcgcgat gacgcagcgg ccggggcggt
     2821 aggaaaccaa gtgattagtg gctagtggct agtggctggc cgccaactct gcgggcggta
     2881 tcgacagcgc cgcccttccc ccgccagtga tgctgcaatc actagccact agccaccagt
     2941 cactagccac tccatgaccg acctttcccg ctatcgcccc aacgtcggcg tcgtgctgtt
     3001 ccatccggac gggcgcgtct ggtacgggcg gc
//
LOCUS       DASVVN010000345         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_460395,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000345 DASVVN010000000
VERSION     DASVVN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460395"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1584
                     /locus_tag="VF686_05580"
     CDS             268..1584
                     /locus_tag="VF686_05580"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX8661316.1"
                     /translation="MRVSRCFLPVLKESPSDAQIVSHKLMLQAGLVRQTAAGIYAWLP
                     IGFRVLKKIEQIVREEQDRAGAIEMLMPTIQSADLWRTSGRYDAYGPEMLRIKDRHDR
                     DMLYGPTNEEMITDIFRQGVQSYKDMPRNLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDIDEASARKAYNRMFVAYLNTFARMGLKAVPMRADTGPIGGDLSHEFIVLADTGE
                     SQVFCDRKLVEMPAPGNAVDWDDLQAIVDERISMYAATEEMHDAGQFETQTAEADRLT
                     ARGIEVGHIFYFGTKYSAPMKARVAGPDGKDTEVHMGSYGVGVSRLLGAIIEASHDDS
                     GIIWPDSVAPFDVVVINLRANDEAVCAACEEAVAKLEALGKDVLYDDTDERPGGKFAT
                     ADLIGIPWQLTIGPKGLADGVVELKRRATGEKLSLTLAEALLKVRG"
     gene            1581..>2000
                     /locus_tag="VF686_05585"
     CDS             1581..>2000
                     /locus_tag="VF686_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8661317.1"
                     /translation="MTDVAATKPTKPAGPFSAWEIELALRYLRARRKEGGIALIAIIS
                     YVAISLAVMALIVVMSIMAGFRSELLSRMLSFNGHMYVQGQILNDPNREAAVTRIGRV
                     PGVVSVSPLTENQSLVRAAGQTTGAFVRGLRPQDLASM"
BASE COUNT          374 a          699 c          613 g          314 t
ORIGIN      
        1 agcaaacgcc cggagcagcc tatgctccgg gcgtcctgcg tgacgaattg aaaacccgtc
       61 agccccctga cgatgggcgg agctaatcgc agttttgcgc acctgtcacc tatcctcacg
      121 caccaagcga gcttttgagc accgaatgtt gcggaccaga cacagtggtt tcgggccaga
      181 aacgccgctc ccccgcattg ccttggccac tctgcagccc tagtggagcg gccaagccac
      241 cagccacagc cggaaggcac ttcaactttg cgcgtttccc gctgtttcct gccggtcctc
      301 aaggaaagcc cgtccgacgc ccagatcgtc agccacaagc tgatgctgca ggcggggctg
      361 gtgcgtcaga ccgcggccgg catctacgcc tggctgccga tcggcttccg ggtactcaag
      421 aagatcgagc agatcgtccg cgaggagcag gaccgcgccg gcgcaatcga aatgctcatg
      481 ccgaccatcc agtcggcgga cttgtggcgg acaagcgggc gctacgacgc ctacgggccg
      541 gaaatgctgc ggatcaagga ccgccacgac cgcgacatgc tctatggccc gaccaatgag
      601 gagatgatca ccgacatctt tcgccaaggc gtccaaagct acaaggacat gccgcgcaac
      661 ctctatcaca tccagtggaa gttcagggac gaggtgcgcc cgcgcttcgg cgtcatgcgc
      721 ggccgcgaat tcctgatgaa ggacgcctat tcgttcgaca tcgacgaggc ctcggcccgc
      781 aaggcctaca accgcatgtt cgtggcctat ctgaacacct tcgcccgtat ggggctgaag
      841 gccgtgccga tgcgcgccga caccggcccc atcggcggcg acctgagcca tgagttcatc
      901 gtcctggccg acacgggcga aagccaagtc ttctgcgacc gcaaactggt cgagatgccc
      961 gcgcccggaa acgccgtcga ctgggacgac ctgcaggcca tcgtcgacga gcgcatctcg
     1021 atgtacgccg cgaccgagga aatgcacgac gccggccagt tcgagaccca gaccgccgaa
     1081 gccgaccgtc tgaccgcgcg cggcatcgag gtcggccaca tcttctattt cggaaccaaa
     1141 tactccgccc cgatgaaggc ccgcgtcgcc gggcccgacg gcaaggacac cgaggtccac
     1201 atgggcagct acggcgtcgg cgtgtcgcgc ctgctgggcg ccatcatcga ggccagccac
     1261 gacgacagcg gcatcatctg gcccgactcc gtcgcgccct ttgatgtcgt ggtcatcaac
     1321 ctgcgcgcca atgacgaggc cgtctgcgcc gcctgcgagg aggctgtcgc caagctggag
     1381 gccctcggca aggacgttct ctacgacgac accgacgaac gcccgggcgg caaattcgcc
     1441 accgccgacc tgatcggcat tccgtggcag ttgaccatcg ggcccaaggg tctcgccgac
     1501 ggcgtggtcg aactgaagcg ccgcgccacc ggcgagaagc tctcgctcac gctggccgaa
     1561 gccctcctca aggtgcgcgg atgacggacg tcgcggcgac gaaaccgacg aagccggcgg
     1621 gcccgttctc ggcctgggag atcgaactgg cgctgcgtta tctgcgcgcc cggcggaagg
     1681 agggcggcat cgccctgatc gccatcatct cctacgtcgc catctcgctg gcggtcatgg
     1741 ccctgatcgt ggtcatgagc atcatggccg gcttccgctc cgaactgctc agccggatgc
     1801 tgtccttcaa cggccatatg tacgtccagg gccagatcct gaacgacccg aaccgcgagg
     1861 ccgccgtcac ccgcatcggc cgcgtcccgg gggtggtcag cgtatcgcct ctcaccgaga
     1921 accagagcct ggtccgcgcc gccggccaga cgaccggcgc cttcgtgcgc ggtctgcgcc
     1981 cgcaagatct ggcttccatg
//
LOCUS       DASVVN010000346         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_145097,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000346 DASVVN010000000
VERSION     DASVVN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145097"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF686_05590"
     CDS             complement(<1..306)
                     /locus_tag="VF686_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8661318.1"
                     /translation="MRIAILTFDGFNELDSFIAAGILNRMRAKGWEARIAAPTPEVTS
                     MNGVAIQRQEPLEFAAEADAVLIGSGIRTREVAADPDLLSRIRLDPSRQLIGAQCSGT
                     "
     gene            495..1187
                     /gene="lexA"
                     /locus_tag="VF686_05595"
     CDS             495..1187
                     /gene="lexA"
                     /locus_tag="VF686_05595"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX8661319.1"
                     /translation="MLTRKQHELLMFIHERIKETGVSPSFDEMKEALDLASKSGIHRL
                     ITALEERGFIRRLAHRARALEVTKLPEQATTSQPRGRQPFRPDVIQGGGRPIAADPAN
                     DTRELSLMGKIAAGTPIAAIEHETARYPVPEAMLGAGEHYMLEIEGDSMIEAGILNGD
                     LVVIKSTGTATSGDIVVALVEGEEATLKRLRKKGGSIALEPANRNYETRIFGPDQVEV
                     QGKLVGLIRRYH"
     gene            complement(1250..>1932)
                     /locus_tag="VF686_05600"
     CDS             complement(1250..>1932)
                     /locus_tag="VF686_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein ComEC"
                     /protein_id="HEX8661320.1"
                     /translation="FLWLAGQGIGLMLAIGEWTAGLPGAVRTVASAPDIALPISFMGV
                     LFCCLWRGPLRWLGLPLAAAVMLWPREPTPDIWIGDGGTNAAWVHAGQTVVMRPGVRQ
                     FAVDVWSRRRGVAPVERPVEGWACTRFACLPEAPAAGPLAMWWGNKPPSPEQIDALCR
                     SAPVVSVRAGLTALPASCRGRLVLDGVDYARGGAAELWRDGPASAGGWRAVWSADVRG
                     DRPWSRPG"
BASE COUNT          347 a          711 c          615 g          259 t
ORIGIN      
        1 cgtgccggaa cactgggcgc cgatcagctg tcgcgaaggg tctagcctga tgcgtgacag
       61 gaggtccgga tcggcggcga cctcgcgcgt gcgaatcccg ctgcctatca gcactgcatc
      121 ggcctcggcg gcgaactcca gcggctcctg gcgctgtatc gcgaccccgt tcatcgaggt
      181 cacctcgggc gtgggtgctg cgatgcgcgc ctcccagccc ttggcgcgca tgcgattcag
      241 aatgccggcg gcgatgaagg aatccagctc gttgaagccg tcgaaggtga gaatggcgat
      301 gcgcacggcg cactccgtaa tggcgcggtc tcccgacccg gggacgctcg cgcccctcat
      361 aagaagccca tcaccacgga aacgcacgcc gaaactcgcc gcgtttcggc gctttgccgt
      421 taagttgaca ttaggcaaga acacttacag aacatccgca acggttcacg ccttgttctg
      481 gacgattcga cgccatgctc acgcgcaaac agcacgaact cctgatgttc atccacgagc
      541 gcatcaagga aacgggggtc tcgccgtcct tcgacgagat gaaggaggcg ctggatctgg
      601 cgtccaagtc cggcatccac cgcctgatca cggcgctgga agaacgcggc ttcatccgcc
      661 gtctggccca ccgggcgcgg gcgctggaag tcaccaaact gccggaacag gccacgacct
      721 cgcagccgcg cggccgccag cccttccgac ctgatgtcat ccagggcggc ggacggccga
      781 tagccgccga tccggccaat gacacgcgcg aactgtcgct gatgggcaag atcgccgccg
      841 gcacgcccat cgccgccatc gaacacgaga ccgcccgcta tccggttccc gaggccatgc
      901 tgggcgcggg cgagcactac atgctcgaga tcgagggcga ctccatgatc gaggccggca
      961 tcctgaacgg cgatctggtg gtcatcaaat cgaccggcac cgccacttcg ggcgacatcg
     1021 tcgtggccct ggtcgagggc gaggaagcca cgctgaagcg gctgcgcaag aagggcggct
     1081 cgatcgccct tgagcccgcc aaccgcaact acgagacccg catcttcggc cccgatcagg
     1141 tcgaggttca gggcaagctg gtcgggctca tccgccgcta tcactgaccc cggatcaggt
     1201 ccggggtgac aaatccggcg cccttggcat cccggaaacg gcgcagccgt tatccgggac
     1261 ggctccacgg ccggtcgccg cgcacgtccg cggaccagac cgcgcgccag cctccggctg
     1321 aggccgggcc gtctcgccac agctcggccg ctccgccgcg agcatagtcg acgccgtcca
     1381 gcaccagccg tcccctgcat gacgctggca gggccgtcag gcccgcacga acgctgacga
     1441 ccggcgcgga tcggcacagg gcgtcgatct gttccggcga cggcggcttg ttgccccacc
     1501 acatcgccaa cggcccggcc gccggagcct cgggtaaaca ggcgaagcgc gtgcaggccc
     1561 agccttcgac gggccgctcg accggcgcta cgccgcgccg ccgggaccag acatcgaccg
     1621 cgaactgccg cacgcccggc cgcatcacca ccgtctgacc ggcgtgcacc caggccgcgt
     1681 tggtcccgcc gtcgccgatc cagatgtcgg gcgtcggctc gcgcggccac agcatcaccg
     1741 ccgccgccag cggcagcccc agccagcgca ggggcccgcg ccacaggcag cagaacaaca
     1801 cgcccatgaa agagatcggc agggcgatgt ccggcgcgct ggccaccgtc cgcaccgcgc
     1861 ccggaagacc agcggtccac tcgccgatgg ccagcatcag gccgattccc tgccccgcca
     1921 gccacaggaa ag
//
LOCUS       DASVVN010000347         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_12833, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000347 DASVVN010000000
VERSION     DASVVN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12833"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VF686_05605"
     CDS             <1..427
                     /locus_tag="VF686_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8661321.1"
                     /translation="RISEPEWEYMKAGAAKAGEAALLAAVTPKVQPFAPGAQVTPSIK
                     AVPLTGHTPGHSGYEIVSGTERLLYVGDALHSSVISVQRPDWVNAWDTDSAAGVATRQ
                     GLLERGATGSQRIYGVHFPFPGLGRFERKDDGFVWVPER"
     gene            450..1580
                     /locus_tag="VF686_05610"
     CDS             450..1580
                     /locus_tag="VF686_05610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8661322.1"
                     /translation="MKAVSTALGGLAALVLMAGPALGQTQALRQGFDIRVGAAPAPVR
                     IGGQTRLVYELQLSNFASTALEVSRIEALDAATGGVIADLQGGALDAALGGSRTVPAN
                     GFVLAYLEAPVEGAAPGRLRHRLTFAGPEGRSITVENGETGIEAAPLPVLGPPLRGGP
                     WVAVYQPDLERGHRRVAYAVDGRVRVPGRFAMDWIKVDETGAQGPSGETRLDSNLSFG
                     AEVLAVADGVVAATRDGVPDPADLNGVDRVALEDATGNYVAIDIGGGRYAFYEHLSPG
                     LRVRPGDRVRRGQVIGALGFTGQASGPHLHFHVSEAPEPLPAEGRPYVLEGFRVVGRY
                     PSIQAFGRGERWGPATEADGEAPSFPAHNVVVWFEDSPTVDR"
     gene            1718..2335
                     /gene="rpsD"
                     /locus_tag="VF686_05615"
     CDS             1718..2335
                     /gene="rpsD"
                     /locus_tag="VF686_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX8661323.1"
                     /translation="MSKRHSAKYKLDRVMGENLWGRAKSPVNKRSYGPGQHGQRRKSK
                     VSDFGLQLKAKQKLKGYYANITEKQFAATYEEASRRKGNSSENLIGLLESRLDAMVYR
                     AKFVPTPWAARQFVSHGHVTVNGKKVDIASYRLKVGDVVEVKEKSRSMALVLEAQQSS
                     ERDIPDYLELGDRGFSVRFVRVPELADVPFPVKMEPNLVVEYYSS"
     gene            2484..2873
                     /locus_tag="VF686_05620"
     CDS             2484..2873
                     /locus_tag="VF686_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661324.1"
                     /translation="MRLIATAAAVMALVPLLSACVVVRQFTNQPRMPESAALPGHVEP
                     VHAAVIAQNQAAFWVSSNGCTAKADIQPVVRQSSDGPIITLRRIKEDRCRQVQPEGVE
                     MRWSFEELGLADGSRLSVENPYQLPPG"
BASE COUNT          476 a          968 c         1050 g          497 t
ORIGIN      
        1 tcgcatcagc gagccggagt gggagtatat gaaggccggc gccgccaagg ccggcgaggc
       61 cgccttgctg gccgcggtga cgcccaaggt ccagccgttc gcaccgggcg cccaggtcac
      121 gccttcgatc aaggccgtgc cgctgaccgg gcacacgccg gggcattcgg gctatgagat
      181 cgtctcggga acggagcggc tgctctatgt cggcgacgcc ctgcacagct cggtcatctc
      241 ggtgcagcgg cccgactggg tcaatgcctg ggatacggac agcgccgccg gcgtggcgac
      301 gcgtcagggc ctgctggagc gcggggcgac cgggtcgcag cgcatctacg gcgtccactt
      361 cccgttcccg ggcctgggcc ggttcgagcg caaggacgac ggcttcgtat gggtaccgga
      421 acgttgagac cgatccgggg gccggggaca tgaaggcagt ttcaaccgcc ttgggcgggc
      481 tggcggcgct ggtcctgatg gccggtccgg ccctcggaca gacgcaggcg ttgcggcagg
      541 ggttcgatat ccgggtgggc gcggcgcccg cgccggttcg catcggcggc cagacgcggc
      601 tggtctatga gcttcagctg agtaatttcg cttccacggc gctggaggtg tcgcggatcg
      661 aggcgctgga cgccgcgacc gggggggtca tcgccgatct gcagggaggc gctctggacg
      721 ccgctctggg cggatcgcga accgtgcccg ccaacggctt tgtgctggcc tatctggagg
      781 cgcccgtaga gggggctgcg cccggccgtc tgcgccatcg tttgaccttt gccggtcccg
      841 agggccgctc gataaccgtc gaaaacggcg agaccgggat cgaggccgcg cccctgccgg
      901 tgctggggcc gcccctgcgc ggcgggccat gggtcgccgt ctatcaaccc gatttggagc
      961 gcggtcaccg ccgggtcgcc tatgccgtcg acggcagggt gcgcgtgccg ggccggttcg
     1021 ccatggactg gatcaaggtc gatgagaccg gggcgcaggg cccttccggc gaaacccggc
     1081 tggacagcaa tctgtcgttc ggcgccgagg ttctggccgt ggctgacggc gtggtcgcgg
     1141 cgacgcggga cggggttccg gatcccgccg acctcaacgg cgtcgaccgc gtggctctgg
     1201 aggacgcgac cgggaactat gtcgcgatcg acatcggcgg cgggcgctac gccttctacg
     1261 aacacctgag ccccggcctg cgggtccggc cgggcgatcg ggtgcggcgc ggtcaggtga
     1321 tcggcgcgct cggtttcacc gggcaggcct caggccctca tctgcatttc cacgtcagcg
     1381 aggcgccgga gcccttgccc gccgagggcc ggccctatgt gctggagggc ttccgggtgg
     1441 tcggacgcta tccctcgatc caggctttcg ggcgcggcga gcggtggggg ccggcgaccg
     1501 aagccgatgg cgaggccccg tccttccccg cccacaacgt cgtagtctgg ttcgaggact
     1561 ctccgacggt tgaccgttga ggcctgatcc gtcataagcc cgccctcacg catccggctt
     1621 gccgggcgcg cgaaccacta cggacgatgc gtggaccgcc gttgagatcg ttccttcatc
     1681 tgggaaggga ccgggccgca tcattagaga gtgacacatg tccaagcgcc atagcgccaa
     1741 gtacaaactc gaccgggtca tgggtgaaaa cctgtggggt cgcgccaagt cccccgtcaa
     1801 caaacgctcg tacggccccg gccagcacgg tcagcgccgc aagtcgaaag tctctgactt
     1861 cggcctgcag ctgaaggcca agcagaagct gaagggctac tacgccaaca tcaccgagaa
     1921 gcagttcgcc gcgacgtacg aggaagcctc gcgccgcaag ggcaacagct cggagaacct
     1981 gatcggcctg ctggagtccc gcctggacgc catggtctat cgcgccaaat tcgttccaac
     2041 cccgtgggcc gcgcgtcagt tcgtcagcca cggccacgtg accgtcaacg gcaagaaggt
     2101 cgacatcgcg tcgtaccgtc tgaaggtcgg tgatgtcgtc gaggtcaagg aaaagtcccg
     2161 cagcatggcg ctggtgctcg aagcccagca gtcgtcggaa cgcgacatcc cggactacct
     2221 cgaactgggc gaccgcggct tctcggtccg cttcgtccgc gtgccggaac tggccgacgt
     2281 gccgttcccg gtgaagatgg agccgaacct ggtcgtggaa tactattcct cctaagtgcg
     2341 gctatcgcgc ggtcgcgcta cttgagccgc gcagtatctg gaaaggcccc ggagaaatcc
     2401 ggggcctttt ctgttccagc cggttccctt acggcgcgaa cgcgcgcatt ttgtcggctg
     2461 agggttcggc gaggaggccg gggatgagac tgatcgcgac agccgccgcc gttatggcgc
     2521 ttgttccgct gctgtcggcc tgcgtggtcg tgcggcagtt cacgaaccag ccgcggatgc
     2581 cggaaagcgc tgccctgccg ggccatgtcg agccggtgca cgccgcggtc attgcccaga
     2641 accaggccgc cttctgggtc agctcgaacg gctgcacggc caaggcggat atccagccgg
     2701 tggtgcgcca gtccagtgat ggaccgatca tcaccctgcg ccggatcaag gaagaccgct
     2761 gccgccaggt ccagccggaa ggcgtcgaga tgcgctggtc gttcgaggag cttggtctgg
     2821 cggacggctc gcggctgtcg gtcgagaatc cctaccaact gccgccgggt tgagtattcg
     2881 agcgaacttc gctgttcgat tgcaaccaat accgtccagc ttacctttaa tggatatgaa
     2941 aactggaagg caatgccgga tcatggccgc ggaagcagcc gggcgcgcct a
//
LOCUS       DASVVN010000348         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_38204, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000348 DASVVN010000000
VERSION     DASVVN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38204"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF686_05625"
     CDS             <1..347
                     /locus_tag="VF686_05625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8661325.1"
                     /translation="DLVGDPETGVLSGGLVTTLLDHIGGLACWVALGRFETLATLDLR
                     VDYMRAAAPRRDLIAEARCYRLTRSVAFVRAWAFEDTVEDPVAAAQSAYMLGSGEGRG
                     AGVNLRAGGSAA"
     gene            344..745
                     /locus_tag="VF686_05630"
     CDS             344..745
                     /locus_tag="VF686_05630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8661326.1"
                     /translation="MTDLLDRLPYARFLGLLTEAAGDSLTVTMPFADKLIGNPILPAL
                     HGGATAALLELTAVAQVAALYPRLRLPRPINVNVAYLRSGRPVDVHASARISRAGRRV
                     AHVLAEAWQDDRTQPIAALTAHFLLAVDELE"
     gene            806..1219
                     /locus_tag="VF686_05635"
     CDS             806..1219
                     /locus_tag="VF686_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661327.1"
                     /translation="MTRQTIGENLAARLYAAEAAIDLALTETAMLAAMLPAARADAYL
                     SAVTGQRAFDGTAGALSALSAARSRMVETHTALAAIARKLGLDVLAVGPIDKPDDTPP
                     IGGGGGDGPGAHPDMVNIIVPNTVNKTLPYTAELC"
     gene            1258..1587
                     /locus_tag="VF686_05640"
     CDS             1258..1587
                     /locus_tag="VF686_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661328.1"
                     /translation="MTKTEVIASVAGDLTATEKAVDEAIARATTLVQAMIGGRTALNI
                     SPVVGAEAQAKAMEAIANLSAARSAVVACHQELAKDHRRLGYGIFAEIAEKGRPEATG
                     EHRLRAV"
     gene            1675..2106
                     /locus_tag="VF686_05645"
     CDS             1675..2106
                     /locus_tag="VF686_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661329.1"
                     /translation="MFETIYAQLVLAVALIVVTFVFLKGDEPERIGGAAYVLLVLATT
                     MIRDGTSLSVPRWGVMGLEIVFLLVFVGLAWHSRRSWPVWAAGFQSLIVTGHVMVALN
                     LRPPTDAWAAVINMANYGILGSMAVGTFWAWQERRAAGLER"
BASE COUNT          332 a          734 c          678 g          375 t
ORIGIN      
        1 aggatctggt cggcgatccg gaaaccggcg tcctgtcggg cggtctggtc acaaccctgc
       61 tggatcacat cggcgggctg gcctgctggg tggcgctggg ccggttcgaa acgctcgcga
      121 ccctcgatct gcgcgtcgac tacatgcgcg ccgccgcccc gcgccgggac ctgatcgccg
      181 aggcgcgctg ctatcgcctg acccgatccg tggccttcgt ccgcgcctgg gccttcgagg
      241 atacggtcga ggatcccgtc gctgcggccc agagcgccta tatgctgggc tccggcgaag
      301 gccgcggcgc gggcgtcaat ctgcgcgccg gagggtccgc ggcgtgaccg accttctgga
      361 tcgcctgccc tacgcccgtt ttctgggtct cctgaccgag gccgctgggg acagcctgac
      421 cgtcaccatg cccttcgcag acaagctgat cggcaacccg atcctgcccg cgcttcacgg
      481 cggcgccacc gcggccctgc tggagctgac cgccgtggcc caggtggcgg ccctgtatcc
      541 acgcctgcgt ctgccccggc cgattaacgt caatgtcgcc tatctgcgct cgggccggcc
      601 tgtggacgtc cacgccagcg cccgcatcag ccgggctgga cgccgcgtcg cccacgtcct
      661 cgccgaggcc tggcaggacg accgcaccca gcccatcgcg gccctgaccg cacacttcct
      721 acttgcagta gacgagctgg agtagcgttc tagtagcgcg catgctcggc ttctccggtg
      781 acgttcggtc accggagaga cgatcatgac cagacaaacc atcggcgaaa acctcgccgc
      841 ccggctctac gcagccgaag ccgccatcga cctcgccctg acagagacag cgatgctggc
      901 ggccatgctg cccgccgcgc gggcggacgc ctacctctcc gccgtcaccg gccagcgcgc
      961 cttcgacggc accgcggggg ctctcagcgc cctgtcggcg gccagatccc ggatggttga
     1021 gacccacacc gccctcgccg ccatcgcgcg caagctgggc ctcgacgtcc tggccgtggg
     1081 ccccatcgac aagcccgacg acacccctcc catcggcggc ggcggcggcg acggtccggg
     1141 ggcgcacccc gatatggtaa atattatcgt accaaacacg gtcaataaaa ccctaccgta
     1201 cacagccgag ttgtgttaaa gagttgttca cctagtattt cctgtgggag gcttttcgtg
     1261 accaagaccg aggtaatcgc cagcgttgcc ggcgacctga ccgcgactga gaaggccgtt
     1321 gacgaggcta tcgcgcgcgc caccaccttg gtgcaggcga tgatcggtgg gcgcacggcg
     1381 ctcaacatct cgcccgtcgt cggcgccgaa gcccaggcca aggccatgga ggcgatcgcc
     1441 aacctgagcg ccgctcgctc tgccgtggtg gcctgccatc aggaactggc aaaggatcac
     1501 cggcgtctgg gttacggtat cttcgccgag atcgccgaaa aaggtcggcc ggaagccacg
     1561 ggcgagcacc gtctccgcgc tgtttaattc gatgtaatgt ccgccagcgg tcatttccgc
     1621 gaaggctgat ctaaggcaat atcgcccccg tgctcagcgc atgggggcga ttctgtgttt
     1681 gaaacgattt acgctcaact cgtcctggcc gtcgcgctta tcgtcgtcac cttcgttttc
     1741 ttgaaggggg acgaaccgga gcggataggc ggcgcggcct atgtgctgtt ggttctcgcc
     1801 accacgatga tccgggatgg caccagcctg agcgtcccgc ggtggggcgt gatgggactg
     1861 gaaatcgtgt ttctgctcgt cttcgtcggc ctggcctggc atagccgccg gtcatggcca
     1921 gtctgggctg ccggctttca aagtttgatc gtcaccggcc acgtcatggt ggcgttgaac
     1981 cttcgcccgc cgaccgatgc ctgggccgct gtcataaaca tggccaacta tggcattctg
     2041 ggatcaatgg ccgtgggcac cttctgggcc tggcaggaac gccgggcggc cggtctggag
     2101 cgctgagccg caccgggcg
//
LOCUS       DASVVN010000349         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_66141, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000349 DASVVN010000000
VERSION     DASVVN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66141"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VF686_05650"
     CDS             <1..678
                     /locus_tag="VF686_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8661330.1"
                     /translation="RRFQTPVQNLIDLNALGPRATIAPGQRLILPATAVDIGGDPYAT
                     GPSPSGVYVPEAGTPPPPPPPPPSGNAALPASRYDPVPTAEPAGPPPAPAAATVSLDW
                     PVRGDILRRFGPVGVGERNNGVNIGAAAGAEVRAAAAGRVGYVGDDLAGQGLTVLVVH
                     RDGWRSVYGHLGSAVVRDGDEVRAGQQIGTVGMSAGDGRPSVHFETWRMRGDQPNAVD
                     PLTVLPR"
     gene            807..1112
                     /gene="yajC"
                     /locus_tag="VF686_05655"
     CDS             807..1112
                     /gene="yajC"
                     /locus_tag="VF686_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX8661331.1"
                     /translation="MAGPFGTLIFFVPVIILFYFMLIRPQQKQMKAHQALVAGLKRGD
                     TVVLSNGMIGKVTRVEADQAMVEIAQGVNVAVVKQMITQVRDRTAIAANDTKTIAKS"
     gene            1143..>1665
                     /locus_tag="VF686_05660"
     CDS             1143..>1665
                     /locus_tag="VF686_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8661332.1"
                     /translation="MIHLSRWKVILLVISLLFGLLFSYPNLLSPAQREALPGWLPKSG
                     LNLGLDLQGGSYLLLEVDVPAMREKRLTNLGEDARTVLAEAKVAVNSIAREDAGVVVT
                     LADPLQMDLALQSMRALVSPGSSGVADRAVQRLGDNRIRYAFTEAALNSMASDAVTQS
                     IEVVRRRIDSLGTR"
BASE COUNT          242 a          590 c          572 g          261 t
ORIGIN      
        1 cggcgcttcc agacccccgt gcagaatctg atcgacctga acgccttggg accgcgcgcg
       61 acgatcgcgc cgggtcagcg gctgatcctg ccggcgacgg cggttgatat tggcggcgat
      121 ccctacgcca ccggcccttc gccgagcggc gtctatgtgc ccgaggccgg aacgccgcct
      181 ccgcctccgc cgccgcctcc gtcgggcaac gccgccctgc ctgcgtcccg ttacgacccg
      241 gtcccgacgg ccgagcccgc gggaccgccc ccggccccgg ccgccgcaac ggtcagtctg
      301 gactggcccg tgcgcggcga catcctgcgg cgcttcggcc ccgtcggcgt gggcgagcgc
      361 aacaacggcg tcaacatcgg ggcggccgcg ggcgccgagg tgcgcgccgc cgccgccggg
      421 cgcgtcggct atgtcggcga cgatctggcg gggcaggggc ttaccgtcct cgtcgtgcac
      481 cgcgacggct ggcgttcggt ctatggccat ctgggttcgg ccgtggtgcg cgacggcgac
      541 gaggtgcgcg cggggcagca gatcggcacg gtcggcatga gcgccggcga cggccggccc
      601 tcggtgcatt tcgagacctg gcgcatgcgc ggggaccagc cgaacgccgt cgatccgctg
      661 accgtgttgc cgcgataacg caaagcggcg ccgtgaacac aggtcgcgtt gcaatgtgtg
      721 acggcgcacc ctagtttccg cgcctcattt tcaagggccg cgtccagcgg ctccagacgc
      781 tcgcggagac catcgtgcag gaacttatgg cggggccgtt cggcaccctg atcttcttcg
      841 tccccgtgat catcctgttc tacttcatgc tgatccggcc gcagcagaag cagatgaagg
      901 cccatcaggc gctggtcgcc ggcctgaagc gcggcgacac ggtcgtcctg tcgaacggca
      961 tgatcggcaa ggtcacccgc gtcgaggccg atcaggccat ggtcgagatc gcccagggcg
     1021 tgaacgtcgc ggtggtcaag cagatgatca cccaggtgcg cgaccgcacc gcgatcgccg
     1081 ccaacgacac caagaccatc gccaagtcct gacgccgttc tgaaaaggtc ggggacgccc
     1141 gcatgatcca tctgtcgcgc tggaaggtca tccttcttgt catctcgctg ctgttcggcc
     1201 tgctgttcag ctatccgaac ctgctgagcc ccgcccagcg cgaggccctg ccgggctggc
     1261 tgccgaaaag cggcctgaac ctcggtctgg accttcaggg cgggtcgtat ctgctgctgg
     1321 aagtcgacgt gccggccatg cgcgagaagc gcctgaccaa cctgggcgag gacgcccgca
     1381 cggttctggc cgaggcgaag gtcgccgtga acagcatcgc ccgggaggac gccggcgtgg
     1441 tcgtcaccct ggccgatccg ctgcagatgg atctggcgct tcagtccatg cgcgccctgg
     1501 tcagcccggg ctccagcggc gtggccgatc gtgcggtgca gcgtctgggc gacaaccgca
     1561 tccgctacgc cttcaccgag gcggcgctga actccatggc ttcggacgcc gtgacccagt
     1621 cgatcgaggt ggtgcgccgc cggatcgaca gcctgggcac gcgcg
//
LOCUS       DASVVN010000350         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_435426,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000350 DASVVN010000000
VERSION     DASVVN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435426"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VF686_05665"
     CDS             complement(<1..733)
                     /locus_tag="VF686_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX8661333.1"
                     /translation="MNAHVSPPVALSQDWDTLDQGKFANETATVRGLLANMPLDDAER
                     AAVLDSAIGLVEHARASVKKQGVVESFLQEFSLGTREGLALMCLAEALLRTPDEDTRD
                     RLIAEKIGSADWSSHLGQSDSLFVNASTWGLMLTGKLVDVDEEARTDLPGFLKRIVGR
                     LGEPVIREAVAAAVRIMGEQFVVGRTIEAALKRANREGWLCSFDMLGEGARTAHDAER
                     YEKIYADAIEAVGKTAKGQGPEAGHG"
     gene            848..1933
                     /locus_tag="VF686_05670"
     CDS             848..1933
                     /locus_tag="VF686_05670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8661334.1"
                     /translation="MRIVLATDAWEPQVNGVVRTLTRTVAECRGMGHEVDVIEPSQFK
                     TIPCPTYPEIRLALGAEEEIRERLRAFEPEAVHIATEGPIGIATRRICVEWKLPFTTS
                     YHTKFPEYVSARFPIPVQVGYAYMKWFHKPSGRLMVATPTLRDELVAHGFRNVSPWTR
                     GVDTEQFNPDLPRIYDELGGKDWPRPFFLNVGRVAVEKNIEAFLETDLPGTKIVVGDG
                     PARAELELKYPDAKFLGARFGAELARCFRDADVFVFPSWTDTFGLVILEAMATGTPVA
                     AYPAHGPIDIIPGSNAGAIDADLRTACLKALECDRTAVRAYAEKFSWRASAEEFVENL
                     QPYPEPERGRFWRRLRRIARLRRKVAA"
     gene            complement(1979..>2792)
                     /locus_tag="VF686_05675"
     CDS             complement(1979..>2792)
                     /locus_tag="VF686_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8661335.1"
                     /translation="PDIANASRPMKASEFELCRANGVTDIVEIKIGFDGIVIATARTG
                     NSFNFRLQDLYTGLAKEVPGAGGAFVANPATTWNQVNPALPNQRIQVYGPPPTSGTRD
                     AFLELGMAPGAELIPALKAIKDADKDRFETLAHTLREDNAWVDSGENDNAIVQTLSRT
                     PGSLGVFGFSFLEQNLDTVKPESIDGVAPSAATIADGTYPLARSLYIYVKKQHVGVTP
                     GLEQFVAEFMSEASAGRGGYLQDRGLVPLPADQLTAQRAVAAAMTPMTAPAK"
BASE COUNT          419 a          919 c          989 g          465 t
ORIGIN      
        1 cgccgtggcc ggcctcgggc ccctgccctt tcgcggtctt gccgaccgcc tcgatggcgt
       61 cggcgtagat cttttcatac cgctcggcgt cgtgggcggt gcgggcgcct tcgcccagca
      121 tgtcgaagct gcacagccag ccttcgcggt tggcgcgctt cagggccgcc tcgatggtgc
      181 ggccgaccac gaactgttcg cccatgatgc ggacggcggc ggcgacggct tcgcggatga
      241 cgggttcgcc cagacggccg acgatgcgtt tgaggaagcc gggcaggtcg gtgcgggcct
      301 cctcgtcgac gtcgaccagc ttgccggtca gcatcaggcc ccaggtcgag gcgttgacga
      361 agaggctgtc cgactggccg aggtgcgacg accagtcggc cgaaccgatc ttttcggcga
      421 tcagccggtc gcgcgtgtcc tcgtccggcg tgcgcagcag ggcctcggcg aggcacatca
      481 gggccagacc ctcgcgggtg ccgagcgaga attcctgcag gaagctttcg accacgccct
      541 gtttcttgac gctggcgcgg gcgtgttcga ccaggccgat ggcgctgtcg aggacggcgg
      601 cgcgttcggc gtcgtccagt ggcatgttgg ccagcaggcc ccgcaccgtg gcggtctcat
      661 tggcgaactt gccctgatcc agcgtatccc agtcctggga gagggcgacc ggcggcgaga
      721 cgtgtgcgtt catggcgcgc gtcctagacg cttttgagcc cgaacgggag ggggcgcggc
      781 atctcgccaa cgcccgtcgg aaccgctaga ccgtttcccc gatcaacggc ggagcctccc
      841 ggcccacatg cgcatcgttc tggccaccga cgcctgggag ccccaggtca acggcgtcgt
      901 ccgcaccctg acccgcaccg tggcggaatg ccggggcatg gggcatgagg tcgacgtcat
      961 cgaacccagc cagttcaaga ccatcccctg ccccacctat ccggagatcc ggctggcgct
     1021 gggagccgag gaagagattc gcgaacggct gcgcgccttc gagcccgagg ccgtccacat
     1081 cgccacggaa ggcccgatcg gcatcgccac gcggcgtatc tgcgtggagt ggaagctgcc
     1141 gttcacgacc agctaccaca ccaaattccc cgaatatgtc tcggcccggt tcccgatccc
     1201 ggtccaggtc ggctacgcct atatgaagtg gttccacaag ccgtcgggcc ggctgatggt
     1261 ggccacgccg acgctgcgcg acgagctggt ggcgcacggc ttccgcaatg tctcgccgtg
     1321 gacgcgcggt gtggacaccg aacagttcaa cccggacctg ccgcgcatct atgacgaact
     1381 gggcgggaag gattggcccc ggccgttctt cctcaacgtc ggccgcgtgg ccgtggagaa
     1441 aaatatcgag gccttcctcg agaccgatct gccggggacc aagatcgtcg tcggcgacgg
     1501 accggcgcgg gccgagctgg aactgaaata tccggacgcc aaattcctcg gcgcgcgctt
     1561 cggcgcggag ctggcgcggt gtttccgcga cgcggacgtt ttcgtcttcc ccagctggac
     1621 cgacaccttc ggcctggtga tcctggaggc catggccacg gggacgccgg tcgcggccta
     1681 tccggcgcat ggtccgatcg acatcattcc gggatcgaac gccggagcca tcgacgcgga
     1741 cctgcgcaca gcctgtctga aggcgctcga gtgcgaccgg accgccgtgc gcgcctatgc
     1801 cgagaaattc agctggcgcg cctcggcgga agagttcgtg gagaatctgc agccctatcc
     1861 cgagcccgaa cggggccggt tctggcgccg gctgcgcaga atcgcccggt tgcgccgcaa
     1921 ggtagcggcg tagcgctcac attctccctc cccatgaagg ggagggagag agcgtttcct
     1981 acttcgccgg agccgtcatc ggcgtcatgg ccgcggcgac ggcgcgctgg gccgtcagct
     2041 gatccgccgg cagcggcacc aggccgcggt cctgcagata gccgccccgg ccggcggagg
     2101 cctcggacat gaactccgcg acgaactgct caaggcccgg cgtcacgccg acgtgctgtt
     2161 tcttgacgta gatgtagagg ctgcgagcca gcggataggt gccgtcggcg atggtggcgg
     2221 ccgacggggc gacgccgtcg atgctctcgg gcttcaccgt gtcgaggttc tgctcgagga
     2281 aggagaagcc gaacacgccc agcgagccgg gggtgcggct gagggtctgg acgatggcgt
     2341 tgtcgttctc gcccgagtcc acccaggcgt tgtcctcgcg cagggtgtgg gccagcgtct
     2401 cgaagcggtc cttgtcggcg tccttgatgg ccttcagggc cgggatcagt tcggcgccgg
     2461 gggccatgcc cagctcgagg aaggcgtcgc gggtgcccga ggtgggcggc gggccataga
     2521 cctggatgcg ctggttcggc agggccggat tgacctggtt ccaggtggtc gccgggttgg
     2581 cgacgaacgc gccgccggcg ccgggcactt ccttggcgag gcccgtgtag aggtcctgaa
     2641 ggcggaagtt gaagctgttc cccgtgcggg ccgtggcgat gacgatgccg tcgaagccga
     2701 tcttgatctc gacgatgtcg gtgacgccgt tggcgcggca cagctcgaac tccgacgcct
     2761 tcatcgggcg cgaggcgttg gcgatgtcag gc
//
LOCUS       DASVVN010000351         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_249313,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000351 DASVVN010000000
VERSION     DASVVN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249313"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..995
                     /locus_tag="VF686_05680"
     CDS             108..995
                     /locus_tag="VF686_05680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8661336.1"
                     /translation="MKAAAALAVAALIGCRPAQAPAPRTALQAEPAPLPVAMPLPGIR
                     VEGRFIQGGLATGTAPDATAALLVDGQAIPLAEGGRFLLGFDRDHGPKAVVEARLADG
                     RTLRREIAVAARTWRIEHVNVAQRPSTASADYLRRRERELARIGAARAQGAQSSGWSQ
                     RFIWPASGRISGVFGSQRVYRGVPAAYHSGVDIAAASGATVVAPADGVVVLAGPPAFS
                     LEGNLVILDHGMGLNSAFLHLSGTVVREGQSVRQGEAIGSVGATGRATGPHLHWSMKW
                     TEARIDPAILTATPAPQGR"
     gene            1276..>2947
                     /locus_tag="VF686_05685"
     CDS             1276..>2947
                     /locus_tag="VF686_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8661337.1"
                     /translation="MIATPAMAQDATNSPGAQSSSPDAEEADETQTPAPTPNTNIPAD
                     DEEIVVTGTIFKTTTSATVSPITVVTAEAMEQRGNNTVQEAIQQLASNNGPALTNSFT
                     ANGAFAAGASAVSLRGLSTNSTLVLFDGQRAAYYPLADDGSRNFVDLNTIPDDVVDRI
                     EVLRDGASSTYGADAIAGVVNIITKREFNGVTARVEAGISEDGNAANQRVSLTAGTGD
                     LDENGMNAYISGFYFKSEGVYNRDLQSPFNTSDERSVCFEDHCGPNNITNVRDDNGVF
                     SGGFLVPFDAYVQPRTGDTNAAIPGARFQLLNPGADCQYGQGFTLTGAEAALPVNATA
                     PFRVCSVDVVNRFQMATPNIERFGATARVTARVGDSNEAYFQVNFQQSTVDYTGFPPV
                     LRGQANAGILFRPFSTSGGPAANLAPGSAELRLPVYVCPTGVGSANGLATGCTAATPG
                     AVLNPNNPFAAQGQLARLLGRPFQTPTQNETQSRVYRAAAGISGTLMEDWDYRVDVTA
                     MHNDLKRTQNGYIYIANLLTAIAQGTINLRQPELNTQAQLDFVMPDNLN"
BASE COUNT          489 a         1072 c          941 g          445 t
ORIGIN      
        1 gccgtcaccg gccagcctat cccgctggag gagctgcgct attggagcgt cgctcggcag
       61 gagccctatg cgagcgccca agcctcggtc caggccgagg cgcggggatg aaggcggcgg
      121 ccgcgctggc cgtggccgcc ctcatcggat gccgaccggc ccaggcgccg gcgcctcgaa
      181 cggcgttgca ggccgagccg gcgccgctac ccgtggcgat gcccctccca gggatccggg
      241 tggaagggcg gttcatccag ggcgggctgg ccaccggcac cgcgcccgac gcgaccgcgg
      301 ccctcctggt cgacggccag gcgatcccgc tggccgaagg cggccgcttc ctcctcggct
      361 tcgaccggga ccacggcccg aaggcagtgg tcgaagcgcg cctcgccgat ggccgaaccc
      421 tccgccgcga gatcgccgtc gccgcccgga cgtggcgcat cgagcatgtg aacgtcgccc
      481 agcggccttc gacggcgagc gccgactatc tgcggcggcg cgagcgcgag ctcgcgcgga
      541 tcggcgccgc tcgggcgcag ggcgcccaaa gctcgggctg gagccagcgc tttatctggc
      601 cggcgagcgg aaggatcagc ggcgtattcg gctcccagcg cgtttaccgt ggcgttccgg
      661 cggcctatca cagcggggtg gatatcgccg ccgcctccgg cgcgaccgtt gtcgctcccg
      721 ccgacggggt ggtagtgctg gccgggccgc cggccttcag cctggagggc aatctggtca
      781 tcctcgacca cggcatgggg ctgaacagcg ccttccttca cctctcgggc accgtcgttc
      841 gggagggtca aagcgttcgc cagggcgaag cgatcggcag cgtcggcgcc accggccggg
      901 ccaccggacc gcatctccac tggagcatga agtggaccga ggccaggatc gacccggcga
      961 ttctgaccgc gactccggcc ccccaagggc gttgatcgcc tcgcacaggg cttgtggcat
     1021 ttttgctaca gcactgcccg aaaccggcca aacaccttaa cccatgcttt acaagctgct
     1081 gaggttcggc gatctgtccc cgatgccatt cggcgccaaa agcgtcggct ggcggggcag
     1141 gcgagcgctg ggggaccgtg tccttcacct cgacaacttc atgagggatc aacggtgact
     1201 cagaaaatca actttacgcg cggcatcctc cggggcggca cagcgctttc cgcactcgca
     1261 ctgctcggcg caggcgtgat cgcaacgccc gccatggcgc aggacgcgac caacagcccg
     1321 ggcgcccagt cgtccagccc cgatgccgag gaagcggacg agacgcagac tccggcgccg
     1381 acgcccaaca cgaacatccc ggccgacgac gaagagatcg tcgttaccgg caccatcttc
     1441 aagacgacga cgtctgccac ggtctcgccg atcacggtgg tcaccgccga agcgatggag
     1501 cagcgcggca acaacaccgt gcaggaagcc atccagcagc tggcctcgaa caacggcccg
     1561 gcgctcacca actccttcac ggcgaacggc gcgttcgcgg cgggcgcctc ggcggtctcg
     1621 cttcgcggcc tgtcgaccaa ctccaccctg gtcctgttcg acggccagcg cgccgcttat
     1681 tatccgctcg cggatgacgg ctcgcgcaac ttcgtcgatc tgaacacgat ccccgacgac
     1741 gtcgtcgacc ggatcgaggt tcttcgcgac ggcgcctcgt cgacctacgg cgcggacgcg
     1801 atcgcgggcg tggtcaacat catcaccaag cgggaattca acggcgtcac ggcgcgcgtc
     1861 gaggccggca tctccgagga cggcaacgcg gccaaccagc gcgtcagcct tactgcgggt
     1921 accggcgatc tcgatgagaa cgggatgaac gcctatatca gcggcttcta tttcaagagc
     1981 gagggcgtct acaaccgcga cctccagtcg ccgttcaaca cctccgacga gcgttcggtc
     2041 tgcttcgagg atcactgcgg cccgaacaac atcaccaacg tccgcgacga caacggtgtg
     2101 ttcagcggcg gcttcctggt tccgttcgac gcttatgtgc agccgcggac gggcgacacc
     2161 aacgccgcca tcccgggcgc tcggttccag ctgctcaacc cgggcgcgga ctgccagtac
     2221 ggccagggct tcaccctgac cggcgctgag gccgcgcttc cggtcaacgc cacggcgcct
     2281 ttccgcgtct gctcggtcga cgtcgtcaac cgcttccaga tggccactcc gaacatcgag
     2341 cgtttcggcg ccacggcccg cgtcacggcg cgcgtcggcg acagcaacga ggcctatttc
     2401 caggtcaact tccagcagag cacggtcgac tataccggct tcccgccggt ccttcgcggc
     2461 caggccaatg ccggcatcct gttccggccg ttctccacct cgggtggtcc tgccgccaac
     2521 ctggcgccgg gttccgccga gcttcgtctc ccggtctacg tctgcccgac gggggtcggc
     2581 tccgccaacg gtcttgccac cggttgtacg gcggccaccc ccggagccgt cctgaacccg
     2641 aacaacccgt tcgcggcgca gggccagctc gcccgtctcc tcggccgtcc gttccagacg
     2701 cccacccaga acgagaccca gagccgcgtc tatcgcgccg ccgcgggcat cagcggcacg
     2761 ctgatggaag actgggatta ccgggtcgac gttacggcga tgcacaacga tctgaagcgc
     2821 acgcagaatg gctatatcta catcgccaat ctgctgacgg cgatcgctca gggcacgatc
     2881 aacctgcggc agccggagct gaacactcag gctcagctcg acttcgtgat gccggacaac
     2941 ctcaaca
//
LOCUS       DASVVN010000352         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_66211, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000352 DASVVN010000000
VERSION     DASVVN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66211"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..2648
                     /locus_tag="VF686_05690"
     CDS             195..2648
                     /locus_tag="VF686_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8661338.1"
                     /translation="MRRHLLARALPAFVLLAAVAGPALAQDGLTYQQPPSPIAEILDT
                     KPTPTPSLSPDDQTLALYDRANLPPIAELAEPMLRLGGYRINPRNNGAANSRVTWLTG
                     LSFQPIAGGARRTVELPAGARFLSPSWSPDGRSLALMMDAPTGLELWTVDVASGRARK
                     LTDARVNAAGGGAFDWLPDSSGLLVRMTPEGRGAAPDVTRPPAGPTVAESMGRAAPAR
                     TYQDLLTDPGDEALFDHYFTSQLTLVPVNGPARTLGAPAVYLGASVSPDGRHVLQTTA
                     KRPYSYVVPAGLFPADVVVTDMNGRVVHRVADLPLRDNIPTPFDAVAPGPRSVQWRSD
                     APATLVWTEAQDGGDPRTAAEVRDRVFTLAAPFNGPPVPLVDLKERYAGVTWGDADTA
                     LVTSRWFNNRHETRYVVDPSNPGSGRVLLERNYQDRYNDPGFPVTEPNDNGFPVIRFA
                     SDGRVLMTGPGATREGEYPFLAAMNLADGQSERLWTSASGDYESVVGFLDEEGRRVVT
                     RRETRNDPPNLFVRDLTAGQPNALTHFPDPAPQLAGVTKQTISYARADGVQLSGELYL
                     PAGYDKDRDGPLPLVMWAYPAEFTDAAVAGQVVDTANRFTRPGGISHLFLLTQGYAVL
                     DNPSMPIVGVNGAEPNDTYIEQLKASAQAAVDAVVGMGVADRDRIAVGGHSYGAFMTA
                     NLLAHTDLFRTGVARSGAYNRTLTPFGFQAEQRTYWEAGDTYTEMSPFTYANKVNEPI
                     LLIHGEADDNSGTFPIQSERFYAALKGQGATVRYVVLPLEAHGYRARESVGHTLWEMT
                     RWLDQYVKNAPPRAAAGAQ"
     gene            complement(2707..3501)
                     /locus_tag="VF686_05695"
     CDS             complement(2707..3501)
                     /locus_tag="VF686_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661339.1"
                     /translation="MQDHQKHRPQDDFGRSSQDDGRWEAGDRRADERVSWSEGDGGRT
                     AEAYRNQGQSDRSYAGSRGMGYGGDQSRYGEGMSTGWTGDRAGGSQGGYGGGSMYGQN
                     HGQDYGRNQQHGFARGGYDPGYSQGGQFGGSSMGGANYGQSYGGGQSQQGQSQQSQGY
                     SRHSYAPGAQIWEGSGPGMGGQTHEHHDFEPDYLHWRQQQLSAFDKDYTDWRNERRQK
                     FSSDFDSWRQTRPRSEASGEHRTPAENPIVGDVSDGGVGTQDAKKR"
     gene            3680..>4309
                     /locus_tag="VF686_05700"
     CDS             3680..>4309
                     /locus_tag="VF686_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX8661340.1"
                     /translation="MAFDEMSGGIPGYGGDVRDSYRGLARWLAEAPDGLLQARSRQAE
                     LFFRRMGITFAVYGDAESSERLIPFDVVPRIIGASEWAGLEAGLIQRVMAMNLFLADV
                     YGPQECLKAGILPPELVLTNPHYVPEMQGRRPPRGVWVHIAGVDLVRTGEDGFYVLED
                     NCRTPSGVSYMLENREMMMRLFPDLFADYPVRPVETYTDMLLASLRACAP"
BASE COUNT          626 a         1505 c         1478 g          700 t
ORIGIN      
        1 ccccgccgat gtctgctcga tcagcgcggc ctatggcgga ccgcacggcg accggcccag
       61 ccgggccagc tgctacgcca tgaccggcgc gaccatcggc ggcagggaag gcggccggcg
      121 gccttgaccc gcgttgagtt cggcccctag cgtgcggcgc cccgggaggg ggcggtccac
      181 accgggaaat caatatgcgt cgtcatctgc tggctcgggc cctgcccgcc ttcgtcctgc
      241 tggccgccgt ggccggaccc gccctggcgc aggacggtct gacgtatcag cagccgccgt
      301 cgccgatcgc ggaaatcctc gacaccaaac ccacgccgac gcccagcctg tcgcccgacg
      361 accagacgct ggccctctac gaccgggcca atctgccgcc gatcgccgaa ctggccgagc
      421 cgatgctgcg gctgggcgga taccggatca atccgcgcaa caacggcgcg gcgaacagcc
      481 gggtgacctg gctgacgggg ctgagcttcc agccgatcgc gggcggcgcc aggcgcacgg
      541 tcgaacttcc ggccggggcc cggttcctgt cgcccagctg gtcgccggac ggccggtcgc
      601 tggctttgat gatggatgcg ccgaccgggc tggagctgtg gaccgtcgac gtggccagcg
      661 gccgcgcgcg caagctgacg gatgcgcggg tcaacgccgc gggcggcggc gccttcgact
      721 ggctgccgga cagctcgggc ctgctggtgc ggatgacgcc cgagggccgc ggcgccgcgc
      781 ccgatgtgac ccgcccgccc gctggcccga cggtggccga gagcatgggc cgggcggccc
      841 ccgcgcggac ctatcaggat ctgctgaccg atccgggcga cgaggccctg ttcgaccact
      901 atttcacctc gcagctgacg ctggttccgg tgaacggccc ggcccggacg ctgggcgcgc
      961 cggccgtcta tctcggcgcc agcgtgtcgc cggacggccg ccatgtgctg cagacgacgg
     1021 ccaaacggcc ctacagctat gtagtgccgg cgggcctgtt cccggcggac gtcgtggtca
     1081 ccgacatgaa cggccgggtg gttcaccgcg tcgccgacct gccgctgagg gacaacattc
     1141 ccacgccgtt cgacgccgtg gcgcccggcc cgcgctcggt gcagtggcgt tctgacgcgc
     1201 ccgcgaccct ggtctggacc gaggcccagg acggcggcga tccgcgcacg gccgccgagg
     1261 tgcgcgaccg cgtcttcacc ctggccgcgc cgttcaacgg tccgccggtc ccgctggtcg
     1321 atctgaagga acgctacgcc ggggtgacct ggggcgatgc ggacaccgcc ctggtcacca
     1381 gccgctggtt caacaaccgg cacgagaccc gatacgtcgt cgatccgtcg aacccggggt
     1441 cgggccgcgt cctgctggag cgcaattatc aggaccggta caatgacccc ggcttcccgg
     1501 tcaccgaacc caatgacaac ggcttccccg tcatccgctt cgcgagcgac ggccgggtgc
     1561 tgatgacggg tccgggcgcg acgcgcgagg gcgaatatcc gttcctggcc gccatgaatc
     1621 tggccgacgg ccagtcggag cggctgtgga cctcggccag cggcgactat gagagcgtcg
     1681 tcggcttcct ggacgaagaa ggccgccggg tggtgacgcg gcgcgagacg cgcaacgatc
     1741 cgcccaatct gttcgtccgc gacctgacgg ccggccagcc caatgccctg acccatttcc
     1801 ccgatccggc gccgcagctg gcaggggtga ccaaacagac gatcagttat gcccgcgccg
     1861 acggggtgca gctgtcgggc gagctttatc tgcccgctgg ctacgacaag gaccgcgacg
     1921 gtccgctgcc gctggtgatg tgggcctatc cggccgagtt caccgacgcc gccgtggccg
     1981 gtcaggtggt cgacaccgcc aaccgcttca cgcgtcccgg cgggatcagc catctgttcc
     2041 tgctgacgca gggatacgcc gtgctggaca atccctcgat gccgatcgtc ggcgtcaacg
     2101 gggccgagcc gaacgacaca tatatcgaac agctgaaggc cagcgcccag gcggcggtcg
     2161 acgccgtcgt cggcatgggc gtggccgacc gcgaccggat cgcggttggc gggcacagct
     2221 acggcgcctt catgaccgcc aacctgctgg ctcacaccga tctgttccgc accggcgtcg
     2281 cccgctcggg ggcctataac cggaccctga cgccgttcgg tttccaggcc gagcaacgca
     2341 cctattggga ggccggggac acctatacgg agatgtcgcc gttcacctac gccaacaagg
     2401 tcaatgagcc gatcctgctg atccacggcg aggccgacga caactccggc accttcccga
     2461 tccagtcgga gcggttctac gccgcgctga aggggcaggg ggcgacggtg cgttacgtcg
     2521 tcctgccgct ggaggcgcat ggctatcgcg cccgggaatc cgtcggtcac accctgtggg
     2581 agatgacgcg ctggctggac cagtacgtca agaacgcgcc gccccgggcg gcggccggcg
     2641 ctcagtaggc gacaaaaaag ggcccggtcg aaaccgggct ctttcgtcac gcgaggttca
     2701 gcgccgtcag cgcttcttcg cgtcctgtgt gccgacgccg ccgtcggaca cgtcgccgac
     2761 gatcgggttt tccgccggtg ttctgtgctc gccagacgct tccgagcgcg gccgcgtctg
     2821 gcgccaactg tcgaagtcgg acgagaattt ctgacgccgc tcgttgcgcc agtccgtgta
     2881 gtccttgtcg aaggcggaca actgctgttg gcgccagtgc aggtagtcgg gctcgaagtc
     2941 gtgatgctcg tgcgtctggc cgcccatgcc gggccccgag ccctcccaga tctgagcgcc
     3001 gggggcgtag ctgtgtcggc tgtagccctg actctgctga ctctgaccct gctgactttg
     3061 gccgccgccg tatgactggc catagttcgc tccgcccatg ctggagccgc cgaactgacc
     3121 gccctggctg tacccggggt cgtagccgcc gcgggcgaag ccgtgctgct ggttgcggcc
     3181 gtagtcctga ccgtggttct ggccgtacat cgagccgccg ccatatccgc cttgcgaccc
     3241 gccagcacgg tcgccggtcc agccggtcga catgccctcg ccatagcggc tctggtcgcc
     3301 gccgtaaccc atgccgcgtg aaccggcgta cgaccggtcc gactgaccct gattgcggta
     3361 tgcttccgcc gtgcgtccgc cgtcgccttc ggaccaggag acgcgttcgt cggcgcgccg
     3421 gtcgccggcc tcccagcggc cgtcatcctg actgctgcgg ccgaagtcgt cctgcggccg
     3481 gtgtttctga tggtcttgca tcggtttcct tccttccaga ggttgggaga cctgaaaacc
     3541 ggacaggcgg cgaggacgtt ccggccatca cgaggacctg acagacttgg ccgtttgaac
     3601 ctttgccgcg tcgcaacatt cctgttgcct cagcgacgct tgtggggcca cgctgaaatc
     3661 tggaagcagc gggagcggca tggccttcga cgagatgtca ggtggaatac ccgggtatgg
     3721 gggggatgtc cgcgacagct atcgcggact ggcccggtgg ctggccgagg cgcccgacgg
     3781 cctgctgcag gcccggtcgc gtcaggcgga gctgttcttc cgccgcatgg ggatcacctt
     3841 cgccgtctat ggcgacgccg aatccagcga gcggctgatc ccgttcgatg tcgtgccgcg
     3901 catcatcggc gcgtcggaat gggccgggct cgaggcgggt ctgatccagc gggtgatggc
     3961 gatgaacctg ttcctcgccg acgtctatgg cccacaggag tgtctgaagg cggggatcct
     4021 gccgcccgaa ctggtgctga ccaatcccca ctatgtgccc gagatgcagg gccgccggcc
     4081 gccgcgcggg gtgtgggtgc atatcgccgg cgtcgatctg gtgcggacgg gcgaggacgg
     4141 cttctacgtg ctcgaagaca actgccgcac cccgtcgggg gtctcctaca tgctggagaa
     4201 ccgcgagatg atgatgcggc tcttccccga cctgttcgcc gactatccgg tgcggccggt
     4261 cgagacctat acggacatgc tgctggcgtc gttgcgggcc tgcgcgccc
//
LOCUS       DASVVN010000353         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_13279, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000353 DASVVN010000000
VERSION     DASVVN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13279"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..952
                     /locus_tag="VF686_05705"
     CDS             173..952
                     /locus_tag="VF686_05705"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX8661341.1"
                     /translation="MLLAIEQGNTNTLFAVHDGTEWVAQWRTATHANRTADEYAVWLS
                     QLLAMRGLAISQLDGCIISSVVPQSIFNLRNLSRRYLSVEPLVIGENVELGMTARITK
                     PSEAGSDRLVNAVGAHMVYPGDLIIIDSGTATTFDVVAADGAFEGGLIAPGINLSLQA
                     LHEAAAMLPRIAIQKPERVIGKDTVSNMQSGVFWGYVALIEGLVARIKAEWGKPMTVI
                     GTGGVASLFEGATESIDRFDPDLTIRGLLEIWRRNTHLKDV"
     gene            949..>2518
                     /locus_tag="VF686_05710"
     CDS             949..>2518
                     /locus_tag="VF686_05710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX8661342.1"
                     /translation="MKKNPNDELVFVPLGGSGEIGMNLNCYGYGPEANRQWLIVDVGV
                     TFGDLSTPGVDVIVPDPAFLDGENIRGIVLTHAHEDHIGALGWLWHRIKAPIYATPFT
                     AFLIREKLREAGILNDVKIIEVPLSGTIDLGPFQVTMITMTHSIAEPNALAIKTPLGT
                     VFHTGDWKLDPTPVIGKPTDIAAITKLGDEGLLAMVCDSTNVFVEGQAGSETDAQAGL
                     IELIGSLKTGRIAVGCFASNVARMVSVMKAAQLAGRRVALAGRSMHRITAAAKHVGLF
                     GDSQRILSEEEARVWPADEILYLCTGSQGEDRAALSRASHGTHPVIKLGSGDHCIFSS
                     RVIPGNELAIAALQDRLADRGVRLYTEKDHPHVHVSGHPCRDEMKQMYLWARPRIAVP
                     THGMRRHLAEHANMAKDLGVAETVTPRNGDMVRLAPGPAAIIDEVPSGRLYVDGGRLV
                     TEQGEALHERRHASTNGVLIVSFAMDKRGKIVSDIDVRAIGLPGDAESPLGDALDDLA
                     ERAEQAVMKLKGEAL"
BASE COUNT          448 a          838 c          840 g          392 t
ORIGIN      
        1 cgatctctcc tcattccgtc accctcgggc ttgtcccgag ggcccatttc ggcaacctcg
       61 ccgtcgttcg ccgggtcggt cggacgtgcg acatgcccga acatccagcc catggaacga
      121 taggccctcg ggacaagccc gagggtgacg ggccaagagg ggaggccgcg taatgctgct
      181 ggcgatcgag cagggcaaca ccaacacgct gttcgcggtg cacgacggga ccgaatgggt
      241 cgcccagtgg cggaccgcga cccacgccaa ccgcacggcc gacgaatacg cggtctggct
      301 gagccaactg ctggccatgc gcgggctggc gatcagccaa ctggacggct gcatcatttc
      361 cagcgtggtg ccgcagtcga tcttcaacct gcgcaacctg tcgcgccgct atctgtcggt
      421 cgaaccgctc gtgatcggcg agaatgtcga actgggcatg acggcgcgga tcaccaagcc
      481 gagcgaggcg ggctctgacc ggctggtcaa cgccgtcggc gcgcacatgg tctatcccgg
      541 cgacctgatc atcatcgaca gcggcacggc cacgaccttc gacgtggtgg ccgccgacgg
      601 ggccttcgag ggcgggctga tcgcgcccgg catcaacctg tccctgcagg ccctgcacga
      661 ggccgccgca atgttgcccc gcatcgccat ccagaagccc gaacgggtga tcggcaagga
      721 caccgtatcc aacatgcaat caggcgtttt ctggggctat gtcgccctga tcgaaggcct
      781 ggtggcgcgc atcaaggcgg aatggggcaa gcccatgact gtcatcggaa ccggcggggt
      841 cgcctcgctg ttcgaaggcg caacagaatc aatcgaccgg tttgatccgg acctcactat
      901 ccgcggcctg ctcgaaatct ggcggcggaa cacccatcta aaggacgtat gaaaaagaac
      961 cccaacgacg aactcgtctt cgttccgctc ggcggatcgg gcgagatcgg tatgaacctg
     1021 aactgctacg gctacgggcc ggaagcgaac cgccagtggc tgatcgtcga cgtcggcgtg
     1081 accttcggcg acctgtcgac gcccggcgtc gatgtgatcg tgcccgatcc ggccttcctc
     1141 gacggcgaga atatccgcgg catcgtcctg acccacgcgc acgaggacca tatcggcgcg
     1201 ctgggatggc tgtggcaccg catcaaggcg ccgatctacg ccacgccgtt caccgccttc
     1261 ctgatccgcg agaagctgcg tgaggctggc attctgaacg atgtgaagat catcgaggtg
     1321 ccgctgagcg gcacgatcga tctggggccg ttccaggtga cgatgatcac catgacccac
     1381 tcgatcgccg agcctaacgc cctggcgatc aagacgccgc tgggcacggt cttccacacc
     1441 ggcgactgga agctggatcc gacgccggtt atcggcaagc ccaccgacat tgcggcgatc
     1501 accaagctgg gcgacgaggg cctgctggcc atggtctgcg actcgaccaa cgtcttcgtg
     1561 gaggggcagg cgggttcgga gacggacgcg caggcggggc tgatcgaact gatcggcagc
     1621 ctgaagaccg gccgtatcgc ggtcggctgt ttcgcttcga acgtggcccg gatggtcagt
     1681 gtgatgaagg ccgcgcagct ggccggacgg cgcgtggcgc tggccgggcg gtcgatgcac
     1741 cggatcacgg cggccgcgaa acacgtcggc ctgttcggcg acagccagcg catcctgtcc
     1801 gaggaggagg cccgcgtctg gccggcggac gagattctgt acctgtgcac cgggagccag
     1861 ggtgaggacc gcgcggccct gtcgcgcgcc tcgcacggca cccatccggt catcaagctg
     1921 gggtcgggcg accactgcat cttctcttct agggtcattc cgggcaacga gctggccatc
     1981 gcggcgcttc aggaccggct cgcggaccgc ggggtgcgcc tctacaccga gaaggaccat
     2041 ccgcacgtcc acgtctccgg ccacccgtgc cgggacgaga tgaagcagat gtacctctgg
     2101 gcccgtccgc gcatcgccgt gccgacccac ggcatgcgcc ggcatctggc cgagcacgcc
     2161 aatatggcca aggacctcgg cgtcgccgaa accgtcacgc cccgcaacgg ggatatggtc
     2221 cgtctggctc ccggccccgc ggccatcatc gacgaggttc ccagcggccg gctgtacgtc
     2281 gacggcgggc gtctggtcac cgaacagggc gaggcgctgc acgagcggcg tcacgcctcg
     2341 accaacggcg tgctgatcgt cagcttcgcc atggacaagc gcggcaagat cgtcagcgac
     2401 atcgacgtgc gcgccatcgg cctgccgggt gacgcggaat cgccgctggg cgatgcactg
     2461 gacgatctgg ccgagcgcgc cgaacaggcg gtgatgaagc tgaagggcga ggcgctgc
//
LOCUS       DASVVN010000354         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_302258,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000354 DASVVN010000000
VERSION     DASVVN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302258"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF686_05715"
     CDS             <1..393
                     /locus_tag="VF686_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8661343.1"
                     /translation="RHELWSPFARPDGKTVGEYGGAVGQGQSYSFSGGRSGQGRIRID
                     AVGPERVVMTLAMLKPIKATNTVEFTLAPRRDGVEVTWAMSGPQTLFGRVLGLFINCD
                     AMCGAQFERGLTDLKTLVETGRQTAIAA"
     gene            473..820
                     /locus_tag="VF686_05720"
     CDS             473..820
                     /locus_tag="VF686_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX8661344.1"
                     /translation="MSYITGFLIPVRNENKDRYVASARKGWEFFKEFGATAQVEGWGE
                     DVPDGKLTSFPMAVKLEEGETVVFSWLVWPDKATADAAFKKMMDDPRMDNMDMPFDGK
                     RMMWGGFTPVFES"
     gene            complement(834..>1609)
                     /locus_tag="VF686_05725"
     CDS             complement(834..>1609)
                     /locus_tag="VF686_05725"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8661345.1"
                     /translation="DGEVTIAVVGKYTGLKDAYKSLIEALHHGGVANDGAVKLDWVES
                     EVFENGYDGAIERLASAHGILVPGGFGQRGSEGKINAVRFAKDRDIPFFGICFGMQMA
                     VIEAARASGVKDASSTEFGPTPDPVVGLLTEWVRGNQLERRSEETEKGGSMRLGAYDA
                     VLAPGSKVAEAYGATSIQERHRHRYEVNIRYKDAIEGAGLKMTGMSPDGLLPEIVERP
                     EHPWFIGVQYHPELKSRPFAPHPLFASFIAAAKEQSRLV"
BASE COUNT          259 a          544 c          555 g          251 t
ORIGIN      
        1 cgccacgagc tgtggtcacc gttcgccagg cccgacggca agacggtcgg tgagtacggc
       61 ggcgccgtcg gccaagggca gagctacagc ttctccggcg gccgctcggg ccagggtcgc
      121 atccgcatcg acgccgtcgg gcccgagcgg gtggtgatga ccctggccat gctgaagccg
      181 atcaaagcca ccaacacggt ggagttcacc ctcgccccac gccgcgatgg cgtggaggtg
      241 acctgggcga tgagcggacc gcagacgctg ttcggccgcg tgctgggcct gttcatcaac
      301 tgcgacgcca tgtgcggcgc ccagttcgaa cgcgggctca cggacctgaa gacgctggtg
      361 gagaccggcc gccagacggc catcgcggcc taggcgaggg ctgtcgcccg gcccactcag
      421 ggaaatgcta gacgtcgccc gcgcagctgg gcacgacccg aaggagaaga gcatgagcta
      481 catcacgggc ttcctgatcc cggtcaggaa cgagaacaag gaccgctacg tcgcctcggc
      541 ccgcaagggc tgggagttct tcaaggagtt cggcgccacc gcacaggtag agggctgggg
      601 cgaggacgtt cccgacggaa agctcaccag ctttcccatg gccgtgaagc tggaggaggg
      661 cgagacggtg gtcttctcct ggctggtctg gccggacaag gcgacggccg acgccgcctt
      721 caagaagatg atggacgacc cccgcatgga caacatggac atgcccttcg acggcaagcg
      781 catgatgtgg ggcggcttca cgcctgtgtt cgagagctga agcggcctgg ccgctagacc
      841 agccggctct gctccttcgc cgcggcgatg aagctggcga acagggggtg cggcgcgaac
      901 gggcggctct tcagctcggg gtggtattgg acgccgatga accacgggtg ctccgggcgc
      961 tcaacgatct cgggcagcag gccgtcgggg gacatgccgg tcatcttcag cccggcgccc
     1021 tcgatggcgt ccttatagcg gatgttgacc tcgtagcggt ggcggtggcg ctcctggatg
     1081 ctggtggcgc cgtaggcctc cgcaaccttg gagccaggcg ccagcaccgc gtcgtaggcg
     1141 cccagccgca tgctgccgcc cttctccgtc tcctcgctgc gccgctccag ctggttgccg
     1201 cgcacccact cggtcagcag ccccaccacc gggtcgggcg tcgggccgaa ctcggtcgac
     1261 gacgcgtcct tcacgcctga ggcgcgcgcc gcctcgatca ccgccatctg catgccgaag
     1321 cagatgccga agaacgggat gtcgcgatcc ttggcgaagc ggacggcatt gatcttgccc
     1381 tccgacccgc gctggccgaa gccgccgggc accaggatgc cgtgcgcgct ggcgagtcgc
     1441 tcgatggcgc cgtcgtagcc gttctcgaac acctcggact cgacccagtc gagcttcacc
     1501 gccccgtcgt tggccacacc gccgtggtgc agggcctcga tgagcgattt gtaggcgtcc
     1561 ttcaggccgg tgtacttgcc gaccaccgcg atggtcacct cgccgtcgg
//
LOCUS       DASVVN010000355         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_407519,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000355 DASVVN010000000
VERSION     DASVVN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407519"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="VF686_05730"
     CDS             complement(<1..1863)
                     /locus_tag="VF686_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8661346.1"
                     /translation="MENFKIDVDADGIALITFDVPGRSMNTLTSGVMAEIPEWVERVK
                     TDDAIKGAVLTSGKASGFCAGADLGDMAGGMLAGGSLQEAFDAGWRLNGALRALETCG
                     KPIAAAINGLALGGGLELTLACHYRVVGDDNKIQLGLPEIKVGLFPGGGGTQRLTRLI
                     GVQAAMMAMTEGKSFRPNDAKGAGFVHEVVARGGEVEAAKAWIKGGGKAVQPWDDKSF
                     KLPGGGPYHPAGIQNFLVGNAMIRKQTYGNYPAATNLMKAVYEGTQVPMDAALRIETR
                     YFIKTLMTPQAQAMIRSLFLSKQELDKGAVRPTGVPKSDPKKVTVLGAGMMGAGIAYV
                     QVMAGIETVLIDQTQEAADKGKAHVADLLKKRLSRGQMTQEKFDATLAMVTATTDYAH
                     VKGSDLVVEAVFESREIKADVTKKAEAQLEPGAIFGSNTSTLPITGLAEASVRPADFI
                     GIHFFSPVDKMMLVEIIMGEKTSQEALARSLDYVLKIKKTPIVVNDSRGFYTSRCFST
                     FLTEGMAMLKEGYAPALIDNVGRMTGMPRGPLEMHDDVALDLSYKIGKQTRADLGDKY
                     VPNPGEDIVATMVEGGRFGRKNGMGFYDYPAEKGSPKTRWKGLAELAPTTKTEGF"
     gene            complement(1875..3080)
                     /locus_tag="VF686_05735"
     CDS             complement(1875..3080)
                     /locus_tag="VF686_05735"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8661347.1"
                     /translation="MADAYIYDAVRTPRGKGKKDGSLHEITGLSLATQVLGALRDRND
                     LDTSKVDDVILGCVTPVGELGADIARTAVLSAGWAQTTAGVQINRFCASGLEAVNMAT
                     AKVKSGEADFAVGGGVEAMSRVPMGSDGGAWPVDPSSAFPTYFVPQGVSADMIASKYG
                     FSRDDVDAYSMESHKRAAAAWADGRFSKSVVPVKDQLGLTILDRDETIRPNTDMQTLG
                     ALNPSFAMMGEMAFDAVIGQRYPEVERITHVHTPGNSSGIVDGSAGVLIGTLEAGKAL
                     GLKPRAKILGGASIGSEPSIMLTGPEFVTRKLLGKLGMTPADIDLWELNEAFAAVVLR
                     YMQALDIDHSKMNVNGGGISMGHPLGATGAMITGIALDELERSNRSTALITLCIGGGM
                     GTATVIERV"
     gene            complement(3348..3722)
                     /locus_tag="VF686_05740"
     CDS             complement(3348..3722)
                     /locus_tag="VF686_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8661348.1"
                     /translation="MIDHLALDVTDIARSRAFYAAALAPLGFRIVAEEKDGEATVVMF
                     GIEGPEFVIADKDRPGEANHVAFRAETRADVDAFHAAALSAGGRDNGPPGIRENYGPT
                     YYAAFVLDPDGFNIEAVCHAPS"
BASE COUNT          626 a         1246 c         1392 g          726 t
ORIGIN      
        1 gaagccttcg gtcttggtgg taggcgccag ttcggcgagg cccttccacc gcgtcttggg
       61 cgaacccttt tcggccgggt agtcgtagaa gcccatgccg ttcttgcggc cgaaccgccc
      121 gccctcgacc atggtcgcga cgatgtcctc gcccgggttg ggcacgtatt tgtcgcccag
      181 atcggcgcgg gtctgtttgc cgatcttgta ggacaggtcc aaggcgacgt cgtcgtgcat
      241 ctccagcggg ccgcggggca tgccggtcat ccggccgacg ttgtcgatca gggcaggagc
      301 atagccctcc ttcagcatcg ccatgccttc ggtcaggaag gtggagaagc agcgcgaggt
      361 gtagaagccg cggctgtcgt tgaccacgat cggggtcttt ttgatcttga gaacgtagtc
      421 gagcgacctg gccaaggctt cctgcgaggt cttctcgccc atgatgatct cgaccagcat
      481 catcttgtcg accggcgaga agaagtggat gccgatgaag tccgcggggc gcaccgaggc
      541 ctcggccagg ccggtgatcg gcagggtcga ggtgttggag ccgaagatgg cgccgggctc
      601 cagctgggcc tcggccttct tcgtcacatc ggccttgatc tcgcggcttt cgaacaccgc
      661 ctcgacgacc agatccgagc ccttcacatg ggcgtagtcg gtggtggcgg tgaccatggc
      721 cagggtggcg tcgaacttct cctgggtcat ctggccgcgt gacagacgct tcttcagcag
      781 gtcggcgacg tgggccttgc ccttgtcggc ggcctcctgg gtctggtcga tcaggacggt
      841 ctcgatgccg gccatgacct gcacataggc gatgcccgcg cccatcatgc cggcgccgag
      901 aacggtgacc ttcttgggat cagacttcgg cacgccggtg gggcggacgg cgcccttgtc
      961 gagctcctgt ttggacagga acaggctgcg gatcatggcc tgggcctgcg gcgtcatcag
     1021 ggtcttgatg aagtagcggg tctcgatgcg cagggcggcg tccatgggca cctgagtgcc
     1081 ttcgtagacg gccttcatca ggttcgtcgc ggccggatag ttgccgtagg tctgcttgcg
     1141 gatcatcgca ttgccgacga ggaaattctg gatgccggcc gggtggtagg ggccgccgcc
     1201 gggcagcttg aaggacttgt cgtcccaagg ctggacggcc ttgccgccgc ccttgatcca
     1261 cgccttggca gcctcgacct cgccgcccct ggccacgacc tcatggacga agccggcgcc
     1321 cttggcgtcg tttggacgga aggacttgcc ctcggtcatg gccatcatgg cggcctgcac
     1381 gccaatcagg cgcgtcagcc gctgggtgcc gccgccgccg gggaagaggc ccaccttgat
     1441 ctcgggcagg ccgagctgga tcttgttgtc gtcacccacg acccggtaat ggcaggccag
     1501 cgtcagctcg agaccgccgc cgagggccag gccgttgatc gccgcggcga tgggtttgcc
     1561 gcaggtctcc agcgctcgca gggccccgtt cagacgccag ccggcgtcga aggcctcctg
     1621 cagcgaaccg ccggccagca tgccgccggc catgtcgccc aggtcggcgc cggcgcagaa
     1681 gccgctcgcc ttgcccgagg tcaggaccgc gcccttgatg gcgtcgtcgg tcttcacccg
     1741 ttcgacccac tcggggatct cggccatcac gcccgaggtg agggtgttca tcgatcgtcc
     1801 ggggacgtcg aaggtgatca gggcgatgcc gtcggcgtcg acgtcgatct tgaagttttc
     1861 catggtcaca gctctcagac gcgttcgatg acggtggcgg tgcccatgcc gccgccgatg
     1921 caaagggtga tcagggcggt cgagcggttg gaccgctcca gctcgtccag ggcgatgccg
     1981 gtgatcatgg cgccggtggc tcccagcggg tggcccatgg agatgccgcc gccgttcacg
     2041 ttcatcttcg aatggtcgat gtcgagcgcc tgcatgtagc gcagcacgac ggcggcgaag
     2101 gcctcgttca actcccacag gtcgatgtcg gcgggagtca tgcccagctt gcccagcagc
     2161 ttgcgggtca cgaactcggg gccggtcagc atgatcgacg gttctgaacc gatggaggcg
     2221 ccgccgagga tcttcgcgcg cggcttgagg cccagcgcct tgccggcttc cagcgtgccg
     2281 atcagcacgc cggccgaacc gtcgacgatg cctgacgagt tgccgggggt gtggacgtgg
     2341 gtgatgcgct cgacctcggg gtagcgctgg ccgatcacgg cgtcgaaggc catctcgccc
     2401 atcatggcga aggacgggtt cagggcgccc aaggtctgca tgtcggtgtt gggacggatg
     2461 gtctcgtcgc ggtcgaggat ggtcaggccc agctggtcct tgaccggcac caccgatttc
     2521 gagaaccggc cgtcggccca ggcggccgcg gcgcgcttgt ggctttccat cgaataggcg
     2581 tcgacgtcgt cgcggctgaa gccgtatttc gaggcgatca tgtcggccga gacgccctgc
     2641 ggcacgaaat aggtcgggaa ggccgaggag gggtcgacgg gccaggcgcc gccgtcggaa
     2701 cccatgggca cgcggctcat cgcctcgacg ccgccgccga cggcgaagtc ggcctcgccg
     2761 gatttcacct tggccgtggc catgttcacg gcttccagac ccgaggcgca gaagcggttg
     2821 atctggacgc cggcggtggt ctgggcccag ccggccgaga ggacggcggt gcgagcgatg
     2881 tcggcgccga gttcgcccac gggcgtgacg cagccgagga tgacgtcatc gaccttcgag
     2941 gtgtccagat cgttgcggtc gcgcagggct cccagcacct gggtggccag gctgaggccg
     3001 gtgatctcgt gcagggaccc gtccttcttg cccttgccgc gcggggtgcg aaccgcgtcg
     3061 tagatatagg cgtcggccat gggagtgcgt ctccaataag gacggattca agggtctccg
     3121 ccacaaaccc tacgcttacg tcaacgtcaa gttacaagcg tggatttggc agggttttga
     3181 gaattcgggg cgccaaccgt gccaaaccct gccacgccgt cgcccgggcg atcccgtcca
     3241 aaccatcgcc gccattgtag aggccgtgcg ggtagtgctc atcaaaatcg ctgcggattt
     3301 ttccaacacc ctgatttgat acgtgttccg ttcgcgcgaa atgtacatca ggacggggca
     3361 tgacagacgg cctcgatgtt gaatccatcc ggatcgagga cgaaggcggc gtaataggtc
     3421 gggccgtaat tctcgcggat tccgggcggg ccgttgtcgc ggccgccggc ggagagcgcg
     3481 gccgcgtgaa aggcatcgac gtcggcgcgg gtttcggcgc ggaaggcgac gtggttggcc
     3541 tcgcccggcc ggtccttgtc ggcgatgacg aactcggggc cttcgattcc gaacatgacg
     3601 acggtcgcct caccgtcctt ctcctcggcg acgattcgaa agccgagcgg ggcgagggcg
     3661 gcggcgtaga aggcgcggga ccgggcgatg tcggtgacgt cgagggcgag gtggtcgatc
     3721 atggcggact ttcctgctcg cggttctgat ccggctgaac gccgaaacgg gccaaacgag
     3781 ccctttgtgt tcgctggcgg acgctgcagg acgctgtttt gcgagaacga ctctcagctg
     3841 ggccgtcggc gggaacgcgg gccttctccg cgccttaagc cgccagacac ctatgtcggc
     3901 aggaggacac accatggccc agacctacga agaaatggac gcgatcgcga tgctgaaggc
     3961 cgatcaccgc aaggtggagg agatcttcgc
//
LOCUS       DASVVN010000356         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_249608,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000356 DASVVN010000000
VERSION     DASVVN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249608"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VF686_05745"
     CDS             complement(<1..113)
                     /locus_tag="VF686_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8661349.1"
                     /translation="MPMLWGRLAVKAFTRSWGRDVMLYTGGVSFFALLAVFP"
     gene            325..>1578
                     /locus_tag="VF686_05750"
     CDS             325..>1578
                     /locus_tag="VF686_05750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8661350.1"
                     /translation="MGSDPRILVVAPDDDLIGPLCQGLDALGWRTVTAHSLAGAIQTL
                     IDWPLEAVILDARLADAGGGVPALRQTVNPRRLPVLAIGADAASWEPGAADIVMSAPP
                     HAAQAALRLENLIRSAIAEEEVSLREATFAARGQPLPLSDLDATPLRVLAAGKPDRHF
                     LALSNALTDRGCEVVAAPTPYTAFDYLHEKPFDAAVLWGAEDHTPALSIASGMKRNTR
                     LYHIPAVLYLRGAGEINLAELYNRGFADVAAADTPEEETAERIVALARAHRRHVAIRK
                     ALESARGSGLTDPATGLFTPELFASHLARIAEGARLRRRPLSVAVLRVSETEAVAEAR
                     KGGWLDRALPQIGAMVSRLIRTEDTAARLSPDVFALALPATHGPSGRIAADRIAAVIG
                     CTAFDAGPDRSPFVVEFEVGVAEVSS"
BASE COUNT          218 a          634 c          495 g          231 t
ORIGIN      
        1 gggaacaccg ccagcagggc gaagaacgac accccgcccg tgtacagcat cacatcgcgg
       61 ccccagctgc gcgtgaacgc cttgaccgcc agccggcccc acaacatggg catggcccat
      121 cgcgccgcga cttcaatatc cgatttggcc tgcaagccgg tccccacaac cgttaacgag
      181 ccgcacttct aacgcaattc ggtcccgtgg aaagcgctgc tggggatagc cggcgccagc
      241 ccgttgctta cagccgccga ccgtcgctat ggtccgcgcc catgaagcag cgcccggggc
      301 tgggcgcggc tggagattcc gatcttgggt tccgatccac gcattctggt cgtcgccccc
      361 gacgacgacc tgatcggccc gctgtgccag gggctggacg ccctcggctg gcgcaccgtc
      421 accgcccatt ccctcgccgg cgccatccag accctgatcg actggccgct ggaagctgtg
      481 atcctcgacg cccgcctcgc cgacgcgggg ggcggggtcc cggcgctccg tcaaaccgtc
      541 aatccgcgcc gcctgcccgt cctcgccatc ggcgccgacg ccgccagctg ggagcccggc
      601 gcggccgaca tcgtcatgtc cgcccccccg cacgccgccc aggccgcgct gcgtctggaa
      661 aacctgatcc gctccgccat cgccgaggaa gaggtcagcc tgcgcgaggc caccttcgcc
      721 gcgcgcggcc agcccctgcc actctccgat ctggacgcca cgcccttacg cgtgctggct
      781 gcgggcaagc cggaccggca tttcctcgcc ctgtccaacg ccctgaccga tcgcggctgc
      841 gaggtcgtgg ccgcgccgac gccctacacc gccttcgact acctgcacga aaagccgttt
      901 gacgccgccg tcctgtgggg cgcggaggat cacaccccgg ccctgtcgat cgcctcgggc
      961 atgaagcgga acacgcgcct gtatcacatt cctgcggtgc tctatctgcg cggcgccggc
     1021 gagatcaatc tggccgaact ctacaaccgc ggcttcgccg acgtcgccgc ggccgatacg
     1081 cccgaggaag agaccgccga gcgcatcgtg gccctggccc gcgcccatcg ccgccacgtc
     1141 gccattcgca aggcgctgga aagcgcgcgc ggttccggcc tgaccgaccc cgccaccggc
     1201 ctgttcacgc ccgaactgtt cgccagtcat ctggcccgca tcgccgaggg ggcccgcctg
     1261 cgccgccgcc cgctgtcggt cgccgtgctg cgcgtctcgg agaccgaggc ggtcgccgag
     1321 gcccgcaagg gcggctggct ggaccgcgcc ctgccccaga tcggggccat ggtctcgcgc
     1381 ctgatccgca ccgaggacac ggcggcgcgt ctgtcgccgg acgttttcgc cctcgccctg
     1441 cccgccaccc acggaccgtc tggccggatc gccgccgacc gcatcgccgc cgtcatcggc
     1501 tgcacggcct tcgacgccgg tcccgaccgc tcgccgttcg tggtcgagtt cgaggtcggc
     1561 gtggccgagg tttcgtcg
//
LOCUS       DASVVN010000357         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_435775,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000357 DASVVN010000000
VERSION     DASVVN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435775"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VF686_05755"
     CDS             <1..1540
                     /locus_tag="VF686_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8661351.1"
                     /translation="FLDHGAKWHGRALVITAAEGGDETIGYPALRDRANRLSGALLAL
                     GLAPGDRIATLAWNSQHHVEIWYAAMGAGLVCHTLNPRLTIAHLAAMIIEAGDRVLAV
                     GAGLAVMAEALVASCPCLERLILLDGDRPMAARGRAPLTDLESLLSDHGAPTAWGQFD
                     ERSLAGLCFTSGTTGAPKGVAYTHRSNYLHTIRSLQADAMALTAEDVVLVAVPMFHAN
                     AWGLPFAAPAVGASMVLPGRVTDGRSLGELINRHGVTVAVGVPTVWLGLLDHLDAIGG
                     ETPTLERIIIGGSSCPDTLLNRMETRLGVTVQTSWGMTELSPLGAISPRSDKGGAARG
                     AGRPPMGLDMLLTDAGGTPLPDQRNALGYLKVRGQSVVQSYFGSSESALDAEGWFDTG
                     DLAIIDEAGNLTLAGRSKDLIKSGGEWINPVEIEEIVGALPSVSLVAVIGSADAKWGE
                     RPLMVIEPAQGQEIEDKALKASLKGRVADWWIPERIVRVAAMPLAATGKINKTQLRAT
                     YGGA"
BASE COUNT          246 a          535 c          570 g          251 t
ORIGIN      
        1 cttcctcgat cacggcgcca aatggcatgg ccgggcgctg gtcatcaccg ccgcggaggg
       61 cggcgacgaa accatcggct atccggccct gcgcgatcgg gcgaaccgcc tgtccggcgc
      121 gcttctagcg ctgggcctcg cgcccggcga ccgcatcgcc accctggcct ggaacagcca
      181 gcaccatgtc gagatctggt acgcggccat gggtgcgggc ctggtctgcc acaccctgaa
      241 tccccggctg acgatcgccc acctcgcggc catgatcatc gaggccggag accgcgttct
      301 ggccgtcggg gctggtctcg ctgtcatggc cgaagcgctg gtggcgtcct gcccgtgcct
      361 cgagcggctg atcctgctgg acggagaccg gccgatggcg gcgcgtggaa gagcgccgct
      421 caccgacctc gaatccttgc tgtcggacca tggtgcgcca acggcttggg ggcagttcga
      481 cgagcggtcg ctggcggggc tgtgcttcac ctccggcacc accggcgcgc cgaagggcgt
      541 ggcctatacg caccggtcga actacctcca caccatccgg tcgcttcagg ccgacgccat
      601 ggccctgacg gccgaggacg tcgttctggt ggcggtgccc atgttccacg ccaacgcatg
      661 gggactgccg ttcgccgcgc cggcggtggg cgccagcatg gtgctgccgg gccgtgtcac
      721 ggacggccga agcctcgggg agttgatcaa ccggcacggc gtcactgtgg ccgtcggcgt
      781 gccgacggtg tggctgggtc tgctggatca tctcgacgcc atcggcggcg aaacgccgac
      841 gctcgagcgc atcatcatcg gcggctccag ctgccccgac actctgctga acaggatgga
      901 aacccgcctc ggcgtcaccg tccagaccag ctggggcatg accgagctgt cgccgctggg
      961 tgccatctcg ccgcgcagcg acaagggtgg cgcggcgcgt ggggcgggcc ggccgccaat
     1021 ggggctggac atgctcctga ccgacgcggg cgggactccg ctgccggacc aacgcaacgc
     1081 gctgggttat ctgaaggtgc gcggccagag cgtggtgcag agctatttcg gatcgtcgga
     1141 atccgcgctg gacgccgagg gctggttcga caccggcgat ctggcgatca ttgacgaggc
     1201 cggcaacctg accctggcgg gccgctcgaa ggacctgatc aagtcgggcg gcgaatggat
     1261 caatccggtc gagatcgagg aaatcgtcgg cgccctgccg tccgtcagcc tcgtcgcggt
     1321 catcggcagt gcggacgcca aatggggcga gcgtccgttg atggtcatcg agccggccca
     1381 gggacaggaa atcgaggaca aggccctgaa ggcgagcctg aaggggcgcg tggccgactg
     1441 gtggattccc gagcggatcg tccgcgtcgc cgccatgccg ctggccgcga cgggcaagat
     1501 caacaagacg cagcttcgcg ccacctatgg gggcgcttag gatgaccgat gtttccgtca
     1561 tcgagtcggc gcgtgcgccg gaccgtcttg aggttcaggt ca
//
LOCUS       DASVVN010000358         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_66741, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000358 DASVVN010000000
VERSION     DASVVN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66741"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF686_05760"
     CDS             <1..262
                     /locus_tag="VF686_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX8661352.1"
                     /translation="VDLAGHKMGDTIRWTDLKGTEDVEPTITDRDFVIASIKTSSAAI
                     SAAADEAHDAEVAAEAAEVEATEQAASGEDAAEAAEGESSEG"
     gene            340..960
                     /gene="pth"
                     /locus_tag="VF686_05765"
     CDS             340..960
                     /gene="pth"
                     /locus_tag="VF686_05765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8661353.1"
                     /translation="MIIIAGLGNPGPKYQGNRHNIGFMAADEIARRWSFGPERAKFQS
                     VIREGEVQTTDGPVKVLLMKPQTYMTESGRAVGEAARFYKVTPAQVIVFHDEIDLAPG
                     RFRMKTGGGAAGQNGVRSLISQLGADFRRARMGVGHPGEPQLVHGHVLSDFHKADQVW
                     LEPMLNAVADALPFAMAGNDERYQAEVLRLAPAPKFSPRQAARGGD"
     gene            1025..1501
                     /locus_tag="VF686_05770"
     CDS             1025..1501
                     /locus_tag="VF686_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX8661354.1"
                     /translation="MTRALVSAATLLMLAACNSGEPAPAPGAPTVTVADALCRPTPKG
                     RQTTGCYMTLTASGEDRLVSVESPEAKYVQIHESRMESNMMMMQQLRDGLPLAAGQAT
                     PLAPGANHLMMLGVEEPLVTGDTVTLKLTFATAAPVEVTARVAQPAAGEYDHVATH"
     gene            1628..>2487
                     /gene="ychF"
                     /locus_tag="VF686_05775"
     CDS             1628..>2487
                     /gene="ychF"
                     /locus_tag="VF686_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX8661355.1"
                     /translation="MALKVAIVGLPNVGKSTLFNALTKTAAAQAANYPFCTIEPNTGD
                     VAVPEPRLEQLAAIAGSKEIIPSRITFVDIAGLVRGASKGEGLGNQFLANIRDCDAVA
                     FVARCFVDDDITHVENRIDPISDLEIIETELMLADLESLEKRVVNVEKKAKGGDKDAQ
                     QTLRLINMALTPLRAGKPARVVEVSKEDEKAWHMLQLLTSLPALYVANVDEGSADKGN
                     ELSDLVAQRAAADNAKSVVISAKIDSELAVLDPEEQAEFLESLGLAEPGLNRLIREAY
                     ALLGLQSYFTV"
BASE COUNT          443 a          887 c          801 g          356 t
ORIGIN      
        1 cgtcgatctg gccggccaca agatgggcga caccatccgc tggaccgacc tgaagggcac
       61 ggaagacgtc gagccgacga tcaccgaccg cgacttcgtg atcgcctcga tcaagacgtc
      121 ttcggccgcc atctcggccg ccgctgatga agcccacgac gccgaggtcg ccgccgaggc
      181 cgccgaggtc gaagccaccg aacaggccgc atcgggcgaa gacgccgccg aagccgccga
      241 gggcgaatcg tcggaaggct gatcgccttc tttcgactgt tttgcgaagc ggccccttcc
      301 ggttacggat ggggccgttt ccttgtctgg acctgagcca tgatcatcat cgccggcctg
      361 ggcaatccgg gtcccaaata ccagggcaac cgccacaaca tcggcttcat ggccgccgac
      421 gagatcgcgc ggcgctggtc gttcgggcct gaacgggcca aattccagtc ggtgatccgc
      481 gagggcgagg tccagacgac tgacggcccc gtcaaggtcc tgctgatgaa gccccagacc
      541 tatatgaccg agagcggccg cgccgtcggc gaggccgcgc gcttctacaa ggtcacgccc
      601 gcgcaggtca tcgtcttcca cgacgagatc gatctggcgc ccgggcggtt ccgcatgaag
      661 accggcggcg gggcggcggg ccagaacggc gtgcggtcgc tgataagcca gctgggcgcc
      721 gacttccgtc gcgcccgcat gggcgtcggc catccgggcg aaccgcagct ggtgcacggc
      781 catgtgctga gcgacttcca caaggccgat caggtctggc tggagccgat gctgaacgcc
      841 gtcgcggacg ccctgccctt cgccatggcc ggaaatgacg aacgctatca ggccgaggtc
      901 ctgcgtctgg cccccgcgcc caaattcagc ccgcgtcagg ccgcacgggg cggcgactag
      961 acgcacccgc cttgtccgcg gtcagcggcg ccgatagacc gcgtccagcc ctgaaggaaa
     1021 cgccatgacc cgcgcgcttg tctccgccgc caccctgctg atgctggccg cctgtaattc
     1081 cggggagccg gcgcccgcgc cgggcgcgcc gacggtcacg gtcgccgacg ccctgtgccg
     1141 gccgacaccc aagggccgcc agaccaccgg ctgctacatg accctgacgg ccagcggcga
     1201 ggaccggctg gtctcggtcg aaagccccga ggccaaatac gtgcagatcc atgagagccg
     1261 catggagagc aatatgatga tgatgcagca gctgcgggac ggcctgccgc tggccgccgg
     1321 tcaggccacg ccgctggcgc cgggcgccaa tcacctgatg atgctgggcg tcgaggagcc
     1381 gctggtcacg ggcgacaccg tgacgctgaa actgaccttc gccaccgcgg cgccggtcga
     1441 ggtcacggcc cgcgtcgccc agccggcggc cggcgaatac gaccacgtcg cgacccactg
     1501 agccgccgaa cctgacaaac aggacgcgac ccgctaaagg ctcgccttca tccctccgct
     1561 ttaccgcctc tcggggaccg gaccgtgcgt ccgcccactg aagaggcttc tggaaccgcc
     1621 caagcccatg gctcttaaag tcgcgatcgt cggcctgccc aacgtcggca agtccaccct
     1681 gttcaacgcc ctgaccaaga cggcggcggc gcaggcggcc aactatccgt tctgcaccat
     1741 cgagccgaac accggcgacg tggctgtgcc ggagccgcgg ctggagcagc tggcggccat
     1801 cgccggctcg aaagagatca tcccctcgcg gatcaccttc gtcgacatcg ccggcttggt
     1861 gcgcggcgcg tccaagggcg agggtctggg caaccagttc ctggcgaata tccgcgactg
     1921 cgacgccgtg gccttcgtgg cccgctgctt cgtggacgac gacatcaccc acgtcgagaa
     1981 ccgcatcgat ccgatctcgg atctcgagat cattgagacc gaactgatgc tggccgacct
     2041 cgaaagcctc gagaagcggg tcgtcaacgt cgagaagaag gccaagggcg gcgacaagga
     2101 cgcccagcag accctgcgcc tgatcaacat ggccctgacc ccgctgcgcg ccggcaagcc
     2161 cgcccgcgtg gtcgaggtgt cgaaggagga cgagaaggcg tggcacatgc tgcagctgct
     2221 gacctccctg cccgccctct acgtcgccaa cgtcgacgag ggctcggccg acaagggcaa
     2281 tgagctgtcc gatctggtcg cccaacgcgc cgctgccgac aacgccaagt ccgtcgtcat
     2341 ctccgccaag atcgattccg aactggccgt gctggacccc gaggaacagg ccgaattcct
     2401 cgaaagcctc ggactggccg agccgggcct gaaccggctg atccgcgagg cctacgccct
     2461 gctggggctc cagtcgtact tcaccgt
//
LOCUS       DASVVN010000359         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_384297,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000359 DASVVN010000000
VERSION     DASVVN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384297"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VF686_05780"
     CDS             <1..761
                     /locus_tag="VF686_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661356.1"
                     /translation="GLALAAGVRSFGLAKELYVLAGLLAGFGLLQLTVALMKGRGVSN
                     GLTEILEDIFAMPKTMRGLAVVQFFSWFALFSMWIYMTPAVTAVHYASPDAASAGYAE
                     GADWVGVLMGIYNGVAALAAFLIPALAARTGLRGAHAVNLTLGAAGFFGLFLIRDPAL
                     LWIPMIGVGFAWASIVSLPYAILANAVPGRKMGVYMGVFNIFIVVPQLVAATLLGATL
                     RTLFADQAIWALVIGGVSFLIAAGASLMVPREDA"
     gene            761..1735
                     /locus_tag="VF686_05785"
     CDS             761..1735
                     /locus_tag="VF686_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8661357.1"
                     /translation="MSPPPQGEGDREAVEGIRRRTLLAGLAALPLATAARADSPTEGR
                     LVEHPQMTSAHVQPRDVTVWLPPGYDASDVRYPVLYMHDGQNLFDGSRAAYGKEWGVD
                     EHVARLSANGQIRTPIVVGVHNTPLRLREYVPADLIRALPEDMRADIQAIYGGEPLSD
                     GYLRFMVEALKPFIDRTYRTRPGRDDTVVMGSSMGGLISLYAMMKHPGVFGAAGCVST
                     HWPIRIDQIKDEAALPPWRERLVAAWTGVIRDGLPDPRGHRLYFDRGDETLDAFYAVF
                     QSRIDQTIRAAGWGPDRFRSLVFPGAEHNEASWNQRLDAPLTFLLPPA"
BASE COUNT          238 a          686 c          531 g          308 t
ORIGIN      
        1 ccggcctcgc cctcgccgcg ggcgtccgca gcttcggcct tgcaaaagaa ctctacgtcc
       61 tcgccggcct gctggccggc ttcggcctgc tgcagctgac cgtcgccctg atgaagggcc
      121 gcggcgtctc caatggactt accgagattc tggaggacat cttcgccatg ccgaagacca
      181 tgcgcggcct tgcggtcgtg cagttcttca gctggttcgc cctgttttcg atgtggatct
      241 acatgacccc cgcagtcacg gccgtgcact acgcctcgcc cgacgccgcc tccgccggct
      301 acgccgaggg cgccgactgg gtcggggtgc tgatgggaat ctacaacggc gtcgcggcgc
      361 tggccgcctt cctcatcccc gcgctggccg cgcggaccgg cctgcgcggc gctcacgccg
      421 tcaatctgac gctgggagcc gccggcttct tcggcctgtt cctgatccga gatccggccc
      481 tgctgtggat tccaatgatc ggcgtcggct tcgcctgggc ctcgatcgtc tccctgccct
      541 acgccatcct cgccaacgcc gtgccgggcc ggaaaatggg cgtctacatg ggcgtcttca
      601 acattttcat cgtcgtgccc cagctggtcg ccgccactct tctcggggcg acgctcagga
      661 ccctgttcgc cgatcaggcc atctgggccc tcgtcatcgg cggcgtcagc ttcctgatcg
      721 ccgccggcgc cagcctgatg gtcccccggg aggacgcgta atgtctcctc cccctcaggg
      781 ggagggggac cgcgaagcgg tggaggggat ccgccgccgt acgcttctcg ccggcctcgc
      841 cgcccttccg ctggcgaccg ccgcccgcgc cgactccccg accgaaggcc gtctggtcga
      901 acacccgcag atgacctcgg cccacgttca gccgcgcgac gtcaccgtct ggctgccgcc
      961 gggatacgac gcctcggacg tccgctaccc cgtcctctac atgcacgacg gccagaacct
     1021 gttcgatggt tcgcgcgccg cctatggcaa ggagtggggc gtcgatgagc acgtcgcccg
     1081 cctgtcagcc aacggtcaga tccgcacgcc catcgttgtc ggcgtccaca acacgccgct
     1141 gcgcctgcgc gagtacgttc ccgccgacct gatccgcgcc ctgcccgaag acatgcgcgc
     1201 cgacattcag gccatctatg gcggcgagcc cctgtccgac ggttatctgc gcttcatggt
     1261 cgaggcgctg aagcccttca tcgaccggac ttaccgcacc cgcccgggcc gcgacgatac
     1321 ggtcgtcatg ggctccagca tgggcgggct gatctcgctc tatgcgatga tgaaacaccc
     1381 cggcgtcttc ggcgccgccg gctgcgtctc gacccactgg cccatccgga tcgatcagat
     1441 caaggacgag gcggcgctgc ccccttggcg cgaacggctg gtcgcggcat ggaccggcgt
     1501 gatccgcgat ggccttccgg acccgcgcgg ccaccgcctg tatttcgacc gcggcgacga
     1561 gaccctggac gccttctacg ccgtcttcca gtcgcgcatc gaccagacca tccgcgccgc
     1621 cggctggggc cccgaccgct tccgcagcct cgtcttcccc ggggccgaac acaacgaagc
     1681 ctcgtggaac cagcgcctcg acgcccccct caccttcctg cttccccccg cctgacgagt
     1741 cccccatgcg catgatcccc cgc
//
LOCUS       DASVVN010000360         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_302511,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000360 DASVVN010000000
VERSION     DASVVN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302511"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1783)
                     /gene="dnaE"
                     /locus_tag="VF686_05790"
     CDS             complement(<1..>1783)
                     /gene="dnaE"
                     /locus_tag="VF686_05790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8661358.1"
                     /translation="RSAYSLLEGAIKAGKVPTLAADAGMPAVGVADRANLFGALEFSE
                     AAKGAGVQPVIACALPVTGIGGKINERWAKVPTVVLLVQNEAGWLNLSALSSSAFLDA
                     GSMDEPSVPWTMVAERSEGLILLSGGPDGPVDPLFVQSRPDEAAAALTEMARVFGDRF
                     YVELQRHGLPSEAQAERGLVEWAYANDAPLVATNDAHYAKAAQARSHDALLCIADGAF
                     TGQEDRRRITGQHWFKPAAAMRELFADLPEACAATIEIARRCAFMAPTRAPILPRFDA
                     GAGRSEPDELMHQAREGLKARLLEVTPAAPEEEYWTRLEWEVGIIQQMGFPGYFLIVS
                     DFIKWAKSHGIPVGPGRGSGAGSLVAWALTITDLDPLRFGLLFERFLNPERVSMPDFD
                     IDFCQERREEVIDYVQDRYGKDRVAQIITFGTLQARAVLRDVGRVLQMPLGQVDRLAK
                     MVPANPANPVTLAQAIEIEPRLREARDNDKAVATLLETALELEGLYRNASTHAAGIVI
                     GDRPLVELAPLYQDPRSTIPASQFNMKWVEAAGLVKFDFLGLKTLTVIDRALKHMAKR
                     GAEVDIGKLPLDDQKSYDLMSAGLTVGV"
BASE COUNT          270 a          592 c          646 g          275 t
ORIGIN      
        1 acacgccgac ggtgagaccg gcgctcatca ggtcgtagga cttctgatcg tcgagcggca
       61 gcttgccgat gtcgacctcg gcgccgcgct tggccatgtg cttgagcgcc cggtcgatga
      121 cggtcagcgt cttgaggccg aggaagtcga acttcaccag accggcggcc tcgacccatt
      181 tcatgttgaa ctggctggcc gggatggtcg agcgcgggtc ctgatacagg ggcgccagct
      241 cgaccagggg gcggtcgccg atgacgatgc cggcggcgtg ggtcgaggcg ttgcggtaca
      301 ggccctccag ctccagcgcc gtctccagaa gggtcgccac ggccttgtcg ttgtcgcggg
      361 cctcgcgcag gcgcggctcg atctcgatgg cctgggccag ggtcacgggg ttggcggggt
      421 tggccggcac catcttggcg aggcggtcga cctggcccag cggcatctgc agcacccggc
      481 cgacgtcgcg cagcacggcc cgcgcctgca gggtgccgaa agtgatgatc tgggccaccc
      541 ggtccttgcc gtagcggtcc tgaacgtagt cgatcacctc ctcccgccgc tcctggcaga
      601 agtcgatgtc gaagtcgggc atggagaccc gttcggggtt caggaagcgt tcgaacagca
      661 ggccgaagcg taagggatcc agatcagtga tggtcagggc ccaggcgacc agcgaaccgg
      721 cgcccgagcc ccggcccgga cccacgggga tgccgtggct cttggcccat ttgatgaagt
      781 ccgacacgat caggaaatag ccggggaagc ccatctgctg gatgatgccg acctcccatt
      841 cgaggcgggt ccagtactct tcctctggcg cggcgggagt gacctccagc aggcgcgcct
      901 tcagtccctc gcgggcctga tgcatcagtt cgtcgggctc ggatcggccc gcgccggcgt
      961 cgaaacgcgg caggatcggc gcccgggtcg gggccatgaa ggcgcagcgc cgggcgatct
     1021 cgatcgtcgc agcacaggcc tcgggcaggt cggcgaacag ctcgcgcatg gccgcggcgg
     1081 gcttgaacca gtgctggccg gtgatgcggc ggcggtcttc ctgaccggtg aaggcgccgt
     1141 cggcgataca cagcagggcg tcgtgggagc gcgcctgcgc cgccttggcg tagtgcgcgt
     1201 cgttggtggc gaccaggggc gcgtcgttgg cgtacgccca ttccacgagg ccgcgttcgg
     1261 cctgagcctc gctcggcagg ccgtggcgct gcagttcgac atagaagcgg tcgccgaaga
     1321 cccgcgccat ctcggtcagg gccgcggcgg cctcgtcggg ccgggactgg acgaacaggg
     1381 gatcgacggg gccgtcgggc ccgcccgaca gcaggatcag cccctcggag cgttccgcca
     1441 ccatggtcca gggcacgctg ggctcatcca tcgagccggc gtcgaggaag gccgaggacg
     1501 acagggcgct gaggttcagc cagcccgcct cgttctggac cagaagcact acggtcggca
     1561 ccttggccca gcgttcgttg atcttgccgc cgatgccggt gacaggcagc gcgcaggcga
     1621 tgacgggttg aaccccggcg cccttcgcgg cctcgctgaa ctccagcgcg ccgaacagat
     1681 tggcccggtc ggcgacgccc accgcgggca tgcccgcatc ggccgccagt gtgggaacct
     1741 tgcccgcctt gatcgcgcct tcgagcagcg aataggccga acg
//
LOCUS       DASVVN010000361         3905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_513374,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000361 DASVVN010000000
VERSION     DASVVN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513374"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="sufB"
                     /locus_tag="VF686_05795"
     CDS             complement(<1..1191)
                     /gene="sufB"
                     /locus_tag="VF686_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8661359.1"
                     /translation="MAAVQQTIDAVAALEKYEHGFTSEIDTEFAPKGLSADIVRFISE
                     KKGEPEWMLEWRLAAYERWLAMEEPTWASVKYTPVDYQDLHYYAAPKEKAGLKSLDEV
                     DPELLAIYAKLGIPLKEQEVLAGVEGAPRYAVDAVFDSVSVVTTFKAELAKVGVIFMP
                     ISEALREHPELVRKYLGSVVPVSDNYFAALNSAVFSDGSFVYIPPGVRCPMELSTYFR
                     INASNTGQFERTLIIADKGSYCSYLEGCTAPMRDENQLHAAVVELVALDDAEIKYSTV
                     QNWYPGDAEGRGGIYNFVTKRADCRGDRSKVSWTQVETGSAVTWKYPSCILKGEESSG
                     EFYSIAITNGHQQADTGTKMIHLGKNSKSRIIAKAVSAGKSDSTYRGLVSVHPKATGV
                     RNFTQ"
     gene            complement(1191..2330)
                     /locus_tag="VF686_05800"
     CDS             complement(1191..2330)
                     /locus_tag="VF686_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX8661360.1"
                     /translation="MNPVYLDYNASGLVRPEVQDAMAKALADNGNPSAVHAAGRRARA
                     RIETARAQVAELVGADAQSVVFSSGGTESNAQAIISAIAAGCERLIVGATEHPCVAEA
                     ATASGKPVKMLPVDARGVVDLNRLRQLLGQPGKAVVAIHHANNESGVIQPIAEAARLV
                     RSAGGWLHVDAIQSAGKIPVDIRELMADSLTLSAHKLGGPQGVGALVLGEGREAVRIL
                     HGAGQERGLRAGTENVPGITGFGMSAVCAARDLPTAQSHAAWRDAAEAVVKKAGATII
                     GEGAERLPNTLFMAVSDWESPQQLITLDLMGLMVSAGSACSSGKTKPSRAILATGLID
                     KATGGIRISGGWGTAEDDWKRFAKAWVEAYAKQRQRAAARSKEDA"
     gene            complement(2327..2800)
                     /locus_tag="VF686_05805"
     CDS             complement(2327..2800)
                     /locus_tag="VF686_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8661361.1"
                     /translation="MRLSTKGRYAVMAMADLAKNGHADGVVRAVSLAEIATRQEISLS
                     YLEQLFARLRKSGLVQSVRGPGGGYRLAKAAQETVIAEIVLAVDEPIRATRCIGHSSP
                     KGCMMAGERCITHNLWEDLGDEIHRYLAGVSLEDVVLNRTGARRRATEAEVGVAA"
     gene            2959..3609
                     /locus_tag="VF686_05810"
     CDS             2959..3609
                     /locus_tag="VF686_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661362.1"
                     /translation="MPEVILPGASGRIEGRYSPGKRPNAPIALILHPHPKANGHMNNP
                     VTVTLYQLFQKRGFATLRYNSRGVGKSQGEFDSGIGELADAATALDWLQAQNPAASQT
                     WVGGYQFGAYIGMQLLMRRPETDGFISVSPPSNMYDFSFLAPCPASGLFLHGTADTVV
                     PPVEVERVVNKLRTQKGIVIDYELEEGATHFWQDHIDAVDRRVGAYLDKRLAEKPA"
     gene            3643..>3905
                     /locus_tag="VF686_05815"
     CDS             3643..>3905
                     /locus_tag="VF686_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661363.1"
                     /translation="MQQDIVIVGGGAGGLELAARLGRRFGPVEGRRRVLLIDRSIFHL
                     WKPTLHEVAAGSLDSHQEGLSYPVLARRNHFSFAFGELAGLDAP"
BASE COUNT          615 a         1433 c         1191 g          666 t
ORIGIN      
        1 ctgggtgaag ttgcgaacgc cggtcgcctt cggatgcacc gagaccagac cgcgataggt
       61 ggagtccgat ttccccgccg agaccgcctt ggcgatgatc cgcgacttcg aattcttgcc
      121 cagatggatc atcttggtgc cggtgtcggc ctgctgatgc ccgttggtga tggcgatcga
      181 atagaattcc cccgagctct cctcgccctt gaggatgcag gatggatatt tccacgtcac
      241 cgccgagccg gtctcgacct gggtccacga caccttcgac cggtcgccgc ggcagtcggc
      301 gcgcttggtg acgaagttgt agataccgcc cctgccctcg gcgtcgccgg ggtaccagtt
      361 ctgcaccgtc gaatatttga tctccgcgtc gtccagcgcg accagttcga ccaccgccgc
      421 atgcagctgg ttctcgtccc gcatcggggc cgtgcagccc tccagatacg agcagtagct
      481 gcccttgtcg gcgatgatca gcgtccgctc gaactggccg gtgttcgacg cattgatgcg
      541 gaaataggtc gacagctcca tcgggcagcg cacgcccggc ggaatgtaga cgaacgaccc
      601 gtccgaaaac accgcgctgt tcagcgccgc gaaatagttg tccgagaccg gcaccaccga
      661 tcccagatat ttccgcacca actcgggatg ctcccgcagg gcctccgaga tgggcatgaa
      721 gatcaccccg accttggcca gctcggcctt gaaggtggtg accaccgaga cgctgtcgaa
      781 caccgcgtcc acggcatacc gtggcgcgcc ctcgacaccc gccagcacct cctgctcctt
      841 cagcgggatg cccagcttgg cgtagatggc cagcagctcg ggatcgacct cgtccagcga
      901 cttcagtccc gccttctcct tgggcgcggc gtagtagtgc aggtcctgat agtcgaccgg
      961 cgtatacttc accgacgccc acgtcggctc ctccatcgcc agccaccgct cataggcggc
     1021 caggcgccac tccagcatcc attccggctc gcccttcttc tcggagatga agcgcacgat
     1081 gtcggcgctc agccccttgg gggcgaactc tgtgtcgatc tcggacgtga agccgtgctc
     1141 gtatttctcg agggccgcga ccgcgtcgat ggtctgttgg acggcggcca tcaggcgtct
     1201 tccttcgacc gcgcggccgc ccgctggcgc tgtttggcat aggcctcgac ccacgccttg
     1261 gcgaaccgct tccagtcgtc ttcggccgtg ccccagccgc cggagatgcg gatgccgccc
     1321 gtggccttgt cgatcagtcc cgtggcgagg atagcccgcg acggcttggt cttgcccgac
     1381 gaacaggccg agccggccga gaccatcagc cccatcagat ccagggtgat cagctgctgc
     1441 gggctctccc agtccgacac ggccatgaac agggtattgg gcagccgctc ggcgccctcg
     1501 ccaataatgg tcgccccggc cttcttgacc accgcctcgg ccgcgtcgcg ccatgcggcg
     1561 tgcgattgtg cggtcggcag gtcacgcgcg gcgcagaccg cgctcattcc gaagcccgtg
     1621 atccccggca cattctcggt cccggcccgc aggccccgtt cctgccccgc gccatgcagg
     1681 atgcgcacgg cctcgcggcc ctcgcccagc accagcgccc cgacaccctg cggcccgccc
     1741 agcttgtgcg cggacagggt caggctgtcg gccatcagct cgcggatatc gaccgggatc
     1801 ttgcccgccg actgaatggc gtcgacgtgc agccagccgc ccgccgaacg caccagccgc
     1861 gccgcctccg cgatcggctg gatgacgccg ctctcgttgt ttgcatggtg tatggccacc
     1921 accgccttgc ccggctggcc cagcaactgc cgcagcctgt tcaggtcgac cacgccgcgc
     1981 gcatcgaccg gcagcatctt gaccggcttg cccgacgccg tcgccgcttc ggccacgcac
     2041 ggatgctcgg tcgcgccgac gatcagccgc tcgcagcccg ccgcgatggc gctgatgata
     2101 gcctgcgcat tggactcggt cccgcccgac gagaacacca ccgactgcgc atcggcgccg
     2161 accagttcgg cgacctgcgc ccgcgctgtc tcgatccgcg cccgcgccct gcgcccggcc
     2221 gcatggaccg ctgacggatt gccgttgtcc gcgagcgcct tggccatcgc gtcctgcacc
     2281 tcgggacgca ccagccccga ggcgttgtaa tcaaggtaca cggggttcac gcggctactc
     2341 caacctcggc ttcggtcgcc cggcggcgcg cgccagtgcg gttcagcacc acgtcttcca
     2401 gcgacacgcc ggccagatag cgatggatct cgtcgccgag gtcctcccac agattgtggg
     2461 tgatgcagcg ctcgccggcc atcatgcagc ccttgggcga cgaatggccg atgcagcggg
     2521 tcgcccggat gggttcgtcg accgccagaa cgatctcggc gatcaccgtc tcctgcgccg
     2581 ccttggccag ccgatagccg ccgccggggc cgcgcacgct ctgcaccagg ccgcttttgc
     2641 gcagcctcgc gaacagctgc tccagatagc tcagcgatat ctcctggcgc gtcgcgattt
     2701 cagccagcga cacggcccgg accacgccgt cggcatgccc gttcttggcc agatcggcca
     2761 tcgccatcac ggcgtatcgt cccttggtcg acagacgcat cgagcacctt caaaaatcgc
     2821 gcgcttttcg agggtcctgt gctagaaccc gcgccttcgc aaaggcgtcg gcgcgcaagc
     2881 tggacttaga aagtcctacc gctgcggtca agtattacgc aggtgcgaaa tgacagttga
     2941 acacggaatt cgccgcctat gcctgaagtc atcctgcccg gcgcctctgg ccgtatcgaa
     3001 ggccgctact cgccgggcaa gcgcccgaac gcgccgatcg ccctgatcct tcatccgcac
     3061 ccgaaggcga acgggcatat gaacaacccg gtcaccgtga ccctgtacca gctgttccag
     3121 aagcgcggct tcgcgacgct gcgctacaac agccgcggcg tgggcaagtc gcagggcgag
     3181 ttcgacagcg gcatcggcga actggccgac gcggccacgg cgctcgactg gctccaggcc
     3241 cagaaccccg ccgccagcca gacctgggtc ggcggctacc agttcggcgc ctacatcggc
     3301 atgcagctgc tgatgcgccg ccccgagacc gacggcttca tctcggtctc gccgccctcg
     3361 aacatgtacg acttcagctt cctggctccc tgcccggcct cgggcctgtt cctgcacggc
     3421 accgccgaca ccgtcgtccc gccggtcgag gtcgagcgcg tggtcaacaa gctgcgcacc
     3481 cagaagggca tcgtcatcga ctacgagctg gaagagggcg cgacccactt ctggcaggac
     3541 catatcgacg ccgtcgaccg ccgtgtcggc gcctatctgg acaagcggct ggccgaaaag
     3601 cccgcctgac cccacatccg gatccgacgc atggagatcc ggatgcaaca ggacatcgtc
     3661 atcgtcggcg gcggcgccgg ggggcttgaa ctggccgccc gtctcggccg ccggttcggc
     3721 cccgtcgagg gccggcggcg cgtgctcctg atcgatcgct ccatcttcca tctgtggaag
     3781 ccgaccctgc acgaggtcgc ggccgggtcg ctggattcac atcaggaggg cctgtcctat
     3841 ccggtgctgg cccgccgcaa tcacttcagc ttcgcgttcg gcgaactggc cggtctcgac
     3901 gcccc
//
LOCUS       DASVVN010000362         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_250056,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000362 DASVVN010000000
VERSION     DASVVN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250056"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VF686_05820"
     CDS             complement(<1..1634)
                     /locus_tag="VF686_05820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX8661364.1"
                     /translation="MNAPFKTPADPLDAPLWDLSDLYASRDDPRIEADLEKARGLVAE
                     LNALQGQLVAARTDAATLGQKLDRAVTLYEQASDVLGGLGAYAFLAASTNRNDAGATG
                     FEATVREKMTAIATPTVWVTLEVNQLEEAEIEAALTAHPAGARWRPWLRRVRAMKPHE
                     LSQELETFLAERGPISAQWPRLFHETLAAMKVRAGKDELTLAEALNRLSDPKAARRKS
                     AAEGLNEALGARTATMAMVMNTVAADKALEDRWRGFKRAADGRHLSNEVDRESVDAMA
                     QAVAAAYPRLSHRYYALKARAMGKATLDQWDRNAPIETSAPRGYDWAAGKQLVLESFA
                     DLGPEFAERASGFFDRPWIDGKARPGKQSGAYAHPVTSDRHPYVFLNWMGERRDVLTL
                     AHELGHGVHQTLAAGQGSLLADTPLTLAETASIFAEGLTFDRLLKTAPKDEQRGLLAG
                     RIEDGLNTVVRQIAFHRFETRFHDERATGEVPVERIGEIWMEEMGASLGPAVTLNAGY
                     EHWWSYVSHFVHSPFYVYAYAFGDLLVAALMETRAK"
     gene            1735..2205
                     /locus_tag="VF686_05825"
     CDS             1735..2205
                     /locus_tag="VF686_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX8661365.1"
                     /translation="MSFRILPLPIEPFRPLFALDDAALEAMGARRMVADTPNSAPCRV
                     SLTDADPGDRLILLNHRHLDHASSPYRSEGPIFVRETAAETAPAPGVVPDMLSSRLLS
                     ARVYDADWMMTDADVVEGRDLARRLEGWFADGTAAHIHLHTARRGCFMAAAERA"
     gene            complement(2202..2636)
                     /locus_tag="VF686_05830"
     CDS             complement(2202..2636)
                     /locus_tag="VF686_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8661366.1"
                     /translation="MTGPAIRTGQAERRQVAALPWRRTGAGLEIMLVTSRETRRWVTP
                     KGGRMPGKTDAEAAAQEALEEAGIEGVTRSEPLGTFRYLKILKRRAPRWCVVAVYALE
                     VQVEHDDWQERGERERVWVSRDEAVRRVDETDLKALIAAFEG"
     gene            complement(2636..>3029)
                     /locus_tag="VF686_05835"
     CDS             complement(2636..>3029)
                     /locus_tag="VF686_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX8661367.1"
                     /translation="LYSRAPPGSEFVVPFWVVITCQAAIALGTLFGGWRIVHTMGSRI
                     TRLSPQQGFCAETGGAITLFLATGLGIPVSTTHTITGAIVGVGAARRASAVRWGVARS
                     IVTAWFITMPASAAIAALFFWIGGAFLT"
BASE COUNT          441 a         1177 c          950 g          461 t
ORIGIN      
        1 tccttcgccc gcgtctccat cagcgcggcc accaacaggt cgccgaaggc gtaggcgtag
       61 acgtagaagg gcgagtggac gaagtggctg acatagctcc accagtgctc gtaacccgcg
      121 ttcagggtca ccgccggtcc cagcgacgcc cccatctcct ccatccagat ctcgccgatc
      181 ctttcgaccg ggacctcgcc cgtcgcacgc tcgtcgtgga accgcgtctc gaaccggtga
      241 aaggcgatct ggcgcaccac ggtgttcagc ccgtcctcga ttcgtccggc cagcaggccg
      301 cgctgctcgt ctttcggcgc ggtcttcagc agccggtcga aggtcagccc ttcagcgaag
      361 atcgaggcgg tctcggccag ggtcagcggc gtatcggcca atagcgaccc ctgccccgcc
      421 gccagcgtct ggtgcacccc atgccccagc tcatgcgcca gcgtcagcac gtcgcgccgc
      481 tcgcccatcc agttcaggaa gacataggga tgccggtccg acgtcaccgg atgggcatag
      541 gcgcccgact gtttccccgg ccgcgccttg ccgtcgatcc atggcctgtc gaagaagccg
      601 ctcgcccgct ccgcgaactc cgggcccaga tcggcgaaac tctcaagcac cagctgcttt
      661 cccgccgccc agtcatagcc acgcggggcg ctcgtctcga tcggcgcgtt ccggtcccac
      721 tggtccagcg tggccttacc catcgcccgg gccttgagcg cgtaatagcg gtgcgacagc
      781 ctcggatagg cggcggccac cgcctgcgcc atcgcatcga ccgactcgcg gtcgacctca
      841 ttggacaggt ggcgcccatc ggcggcccgc ttgaagccgc gccagcggtc ctccagcgcc
      901 ttgtcggccg cgaccgtgtt catcaccatc gccatggtcg ccgtccgcgc gcccagcgcc
      961 tcgttcagtc cctcggccgc cgacttgcgc cgcgccgcct tcgggtccga cagccggttc
     1021 aacgcctcgg ccagcgtcag ttcgtccttg cccgccctga ccttcatcgc cgccaaagtc
     1081 tcgtggaaca ggcgcggcca ctgggcgctg atcggtcccc gctcggcgag gaaggtctcc
     1141 agttcctggg acaactcatg cggcttcatc gccctcaccc gccgcagcca cggccgccag
     1201 cgcgccccgg ccggatgcgc ggtcagggcg gcctcgatct cggcctcctc cagttggttc
     1261 acctccagcg tcacccagac cgtcggcgtg gcgatggcgg tcatcttctc gcgcaccgtg
     1321 gcctcgaatc ccgtcgcgcc ggcgtcgttc cggttcgtcg acgccgccag gaaggcatag
     1381 gcccccagcc cgcccagcac gtccgacgcc tgttcgtaca gggtcacggc ccggtccagc
     1441 ttctggccca gcgtcgccgc atccgtccgc gccgcgacca gctggccctg cagggcgttc
     1501 agctcggcca ccagccccct cgccttctcc agatcggcct cgatccgggg gtcatcgcgc
     1561 gaggcgtaga ggtcggacag gtcccacagc ggggcgtcca gcggatcggc gggggtcttg
     1621 aagggtgcgt tcatgctctc cgttgtgggg atgccgcgcg ccccgcgcaa cgtccgaaaa
     1681 ccgccagacg catgatctgc cgcccctcat accggcggcg aaggagatca ccccatgagc
     1741 ttccgcatcc tgcccctgcc gatcgaaccg ttccggccgc tgttcgccct cgacgatgcc
     1801 gctctggaag cgatgggcgc ccgccgcatg gtcgccgaca cgccaaacag cgcaccgtgc
     1861 cgcgtcagcc tgaccgacgc cgatccgggc gaccggctga tcctgctgaa ccaccgccat
     1921 ctggaccacg cctccagccc ctaccgatcc gaaggcccga tcttcgtccg cgagacggcc
     1981 gccgaaacgg cgcccgcgcc gggggtcgtg cccgacatgc tgtccagccg ccttctgtcc
     2041 gcccgcgtct atgacgccga ctggatgatg accgacgccg acgtggtcga gggccgcgat
     2101 ctggcgaggc ggctggaggg gtggttcgcc gacgggaccg ccgcccatat ccacctgcac
     2161 accgcccggc gcggctgctt catggccgcc gctgagcgcg cctagccttc gaatgcggcg
     2221 atcagggcct tcagatcggt ttcgtcgacc cggcgcacgg cctcgtcccg gctgacccag
     2281 acccgctcac gctcgccgcg ctcctgccag tcgtcgtgct cgacctggac ctcgagcgcg
     2341 tagaccgcga ccacacacca gcgcggcgcc cggcgtttca gaatcttgag atagcggaac
     2401 gttcccagcg gctcggaacg ggtcacgccc tcgatccccg cctcctccag cgcctcctgc
     2461 gccgcggcct ccgcgtcggt cttgcccggc atacggccgc ccttgggcgt gacccagcgc
     2521 cgggtctcgc gcgaggtgac cagcatgatc tccagccccg ccccggtcct ccgccatggc
     2581 aaggccgcga cctgacgtcg ctcggcctgc ccggttcgga tcgcgggacc ggtcatcagg
     2641 tgaggaaggc tccgccgatc cagaagaaca gcgccgcgat ggccgccgag gccggcatgg
     2701 tgatgaacca ggccgtgacg atgctgcgcg ccacgcccca gcgcaccgcg gaggcccgtc
     2761 gcgccgcgcc gacgccgacg atggcgccgg tgatggtgtg ggtggtcgag acgggaatgc
     2821 ccagaccggt ggccaggaac agggtgatcg ccccacccgt ctcggcgcag aatccctgct
     2881 gcgggctcag ccgcgtgatg cgcgagccca tggtgtggac gatccgccag ccgccgaaca
     2941 gcgtgcccag cgcgatggcc gcctgacagg tgatcaccac ccagaacggc accacgaact
     3001 cggaccccgg aggcgcccgc gaatacagc
//
LOCUS       DASVVN010000363         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_92904, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000363 DASVVN010000000
VERSION     DASVVN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92904"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="VF686_05840"
     CDS             <1..1674
                     /locus_tag="VF686_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661368.1"
                     /translation="ADYSFEDGWIRSLRFGGRFAKREQTTRFSTYNWGVLSEQWGDNG
                     PVWLSDLVDGVPNAGGAPGAVGFNPMDYNETFSFDNFMRGEVQSPTGDQPRLFFNRNI
                     IDEYAAYAAYGLAVGDEWRARLNGAGCPQNWVPLALRCNADADGFLRQEINPMQEETA
                     SGYAMLRFGNEFSNGWNLTGNVGLRYVTTDRAAQGFQAFNLVSFPDEATCAVPVPPPA
                     QPVPFCTLAPSVRADARLFANGAVTAADTETSFDYWLPSFNAKLEVGNGWQFRFGFSQ
                     AITPPDVGLTRNYYNIALSANQQDIVDGRPTGRFAVGNPYLKPIRSTNFDASAEWYFA
                     AVGQLTLSLFYKELDDVITNGTERLSFTNNGATFDSVVTTPVNSEDTGTVKGFELAYQ
                     QTYDFLPDPFDGLGISANYTYIDSSGVAQSTLSSTDPDVAAGRVANIDTSLLPLQNLS
                     EHTVNFAGFYEKGRLSARLAYTWRSEFLITPRDVIVPFAPIMNEDTGQLDGSIFYTLN
                     DEWKIGLQAVNLTNEITRTSQVLNNDLLRAPRSWFMNDRRFTLIARATF"
     gene            1727..2866
                     /locus_tag="VF686_05845"
     CDS             1727..2866
                     /locus_tag="VF686_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX8661369.1"
                     /translation="MLTRRQTLATAAGSLVAACSGLPARAETESLQQLAARSGRRFGS
                     TVGAGRALTGSLGDSGYRDLLIRECGLLVPENELKWYALRPDAETFNYAPADRIADFA
                     REHGMGMRGHTLLWHHPQWFPAWAEGHDYGPRPGSTVERLLVGHVDAVVDRYGALIDS
                     WDVVNEAVDANTGALRETAFSRRMGGPEAVMDLAFHTARAAAPGVQLVYNDYMSWEAG
                     HETHQAGVLRLLEGFKARGVPVDALGVQSHIGSGNTDGSVGFDTARENNWRRFLDTVV
                     AMGYDLVITEFDVHDKNLPVDPAVRDAAVAGLAHRYLDLMLSYPQMGDVMCWGICDRY
                     SWLQNIWPRADRLPKRPTPYDADYRAKPLRAAIADALRNAPPRAA"
     gene            2952..>3362
                     /locus_tag="VF686_05850"
     CDS             2952..>3362
                     /locus_tag="VF686_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-D-glucan glucohydrolase"
                     /protein_id="HEX8661370.1"
                     /translation="MIRTSFRISALALAVALGGSVAWAQSAAPSVPEAARAHPALWPQ
                     AASPAAMTDARTEAFVAGLMARMTLEEKVGQLIQADISTITPEDMLTYPLGSILAGGS
                     SGPWGDDTAPPERWLALAHAFREANARRGGTVIPL"
BASE COUNT          531 a         1186 c         1104 g          541 t
ORIGIN      
        1 gcggactaca gtttcgagga cggctggatc cggtcgctgc ggttcggcgg acgcttcgcc
       61 aaacgggagc agacgacgcg gttcagcacc tacaactggg gcgtcctgtc cgaacagtgg
      121 ggcgacaacg gtccggtgtg gttgagcgac ctggtcgacg gcgtgcccaa cgccggcggc
      181 gcgcccggcg cggtcggatt caatccgatg gactacaacg agaccttctc gttcgacaac
      241 ttcatgcgcg gcgaggttca gtcgccgacc ggagatcagc cccgcctgtt cttcaaccgc
      301 aacatcatcg acgaatacgc cgcctacgcc gcctacggcc tggccgtcgg cgacgagtgg
      361 cgggcgcgtc tgaacggggc cggctgcccg cagaactggg tgcctctggc gctgcgctgc
      421 aatgcggatg ctgacggttt cctgcggcag gaaatcaatc cgatgcagga ggagaccgcc
      481 tccggctatg caatgctgcg gttcggaaac gagttctcga acggctggaa cctgaccggc
      541 aacgtcggcc tgcggtacgt caccacggac cgcgcggcac aagggttcca ggccttcaat
      601 ctcgtcagtt tcccggacga ggcgacctgc gccgtacccg tgccgccgcc ggcgcagccg
      661 gtgccgttct gcaccctggc gcccagcgtc agggccgacg cccggctttt cgcaaacgga
      721 gcggtcaccg cggccgacac cgaaacctcg ttcgattact ggctgcccag cttcaatgcg
      781 aagctggagg tcggcaacgg ctggcagttc cgcttcggct tctcgcaggc catcacgccg
      841 cccgacgttg gcctgacccg gaactactac aacatcgccc tttcggcgaa ccagcaggac
      901 atcgtcgatg ggcgtccgac gggacggttc gcggtcggca atccctatct gaagccgatc
      961 cggtcgacca acttcgacgc ctcggcggag tggtatttcg ccgccgtggg ccagctgacc
     1021 ctctcgctct tctacaagga gctggacgac gtcatcacca acggcaccga gcgactgtcc
     1081 ttcaccaaca acggcgccac cttcgattcg gtggtgacga cgccggtcaa ctcggaagac
     1141 accgggaccg tgaagggctt cgaactcgcc tatcagcaga cctacgactt cctgccggat
     1201 ccgttcgacg gtctggggat cagcgccaac tacacctaca ttgactcgtc cggcgtggcg
     1261 cagagcaccc tgtcgtcgac cgatcctgac gtggccgccg ggcgtgtcgc caacatcgac
     1321 accagcctgc tgccgctcca gaacctgtcc gagcacacgg tcaacttcgc cggcttctac
     1381 gagaagggac ggctttcggc ccgcctcgcc tacacctggc ggtccgagtt cctgatcacg
     1441 ccgcgggacg tcatcgtgcc gttcgcgccg atcatgaacg aagacaccgg gcagctggac
     1501 ggttcgatct tctacacgct caacgacgag tggaagatcg gcctgcaggc ggtcaatctg
     1561 accaacgaga tcacccgcac gtcacaggtc ctgaacaacg acctgcttcg ggcgccgcga
     1621 tcctggttca tgaacgatcg ccggttcacc ctgatcgccc gggcgacgtt ctaggtctcc
     1681 accctggggc gccgcggtcg gcgcgcccct gctttttcga ggaccgatgc ttactcgccg
     1741 acagaccctg gccaccgccg ccggcagtct ggtcgccgca tgctccggcc tgcccgcgcg
     1801 ggcggagacg gaaagcctgc agcagctggc cgcccggtcc ggccgccgct tcggcagcac
     1861 ggtcggggcg ggccgggcgc tgacgggctc gctcggggac tcgggctacc gcgacctcct
     1921 catccgggaa tgcggcctgc tggtgcccga gaacgagctg aaatggtacg cgctccgccc
     1981 cgacgcggag accttcaact acgcgcccgc cgaccgcatc gccgacttcg cgcgggagca
     2041 cgggatgggc atgcgggggc ataccctgct gtggcaccat ccgcagtggt tccccgcctg
     2101 ggccgagggt catgactacg gcccgcgccc gggctcgacc gtcgagcggc tgctggtcgg
     2161 gcacgtcgac gccgtggtgg accggtacgg cgccctgatc gattcatggg atgtcgtgaa
     2221 cgaggcggtc gacgccaata cgggcgccct gcgcgaaacc gccttctcgc ggcgcatggg
     2281 gggacccgag gcggtcatgg acctggcctt ccacaccgcc cgcgccgcgg cgcccggggt
     2341 ccagctggtc tataacgact acatgagctg ggaggccggg cacgagacgc atcaggccgg
     2401 cgtgcttcgc cttctcgagg gcttcaaggc gcgcggcgtg cccgtcgacg cgctgggggt
     2461 ccagagccac atcggctcgg gcaataccga cgggtccgtc ggtttcgaca cggcgcgcga
     2521 gaacaactgg cgccgcttcc tcgatacggt cgtggccatg ggctacgatc tggtcatcac
     2581 cgagttcgac gtccacgaca agaatctgcc ggtcgatccc gctgtccgcg acgcggccgt
     2641 ggccgggctg gcccaccgct atctggatct gatgctgtcg tatccgcaga tgggggacgt
     2701 gatgtgctgg ggcatttgcg accgctattc ctggctgcag aacatctggc cgcgcgcgga
     2761 tcgcctgccc aagcggccga ccccctacga cgccgactac cgggccaagc cgctgcgcgc
     2821 cgcgatcgcc gacgccttgc gcaacgcccc gccgagagcc gcctgagccg gcggccttgc
     2881 cgggtcccgc agctcaacgg aaagatatcg ccttgccggg gccccggtcc cgcagccgcc
     2941 tcgaggaccg catgatccgc acgtcgttcc gtatttcagc cctggccctg gctgtcgccc
     3001 tcggcggatc cgtcgcctgg gcccagtccg cggcgccctc ggttccggag gccgcccggg
     3061 ctcatccggc cctgtggcca caggccgctt cgcctgcggc gatgacggac gcccggactg
     3121 aggccttcgt ggccgggctg atggcgcgga tgacgctgga ggagaaggtg ggccagctga
     3181 tccaggccga catttccacg atcaccccgg aagacatgct gacatatccg ctgggttcga
     3241 tcctggcggg cggcagttcg ggaccgtggg gcgacgacac ggcgccgccc gagcgctggc
     3301 tggcgctggc ccacgccttc cgcgaggcca acgcccgccg cggcgggacg gtgatccctc
     3361 tg
//
LOCUS       DASVVN010000364         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_278520,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000364 DASVVN010000000
VERSION     DASVVN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278520"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF686_05855"
     CDS             complement(<1..219)
                     /locus_tag="VF686_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661371.1"
                     /translation="MTDAAETRAGPGLKTVVTASAAGTAFEWYDFFVFGSLTTIIARH
                     FFADAGETVAYIMALLTFGLGFVVRPLGA"
     gene            complement(388..525)
                     /locus_tag="VF686_05860"
     CDS             complement(388..525)
                     /locus_tag="VF686_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin EcnA/B family protein"
                     /protein_id="HEX8661372.1"
                     /translation="MRKLFVLFAAAAALTTAACNTVSGVGRDASAAGSAVTTAAEDAK
                     N"
     gene            complement(566..>1887)
                     /locus_tag="VF686_05865"
     CDS             complement(566..>1887)
                     /locus_tag="VF686_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX8661373.1"
                     /translation="VLARAPTVAGRSPGLIVAIRLERPLGAFDGAMVALLPLASLQPD
                     VDDPALPEGSQAALTDGDGRLLTATEADAFVLSGGQSLTGWVGRARSDASAIFEADDA
                     RGRHRVYAGAALAGRDVYALLSAPAPGLLSWARLNPVGALLMPLLAWFIAFGAVMLVS
                     ERIVIRWLNYLERIAAIYARGRFNVRPLQAMNAPAEIRVLARTLDELAESIVTRDAAL
                     TASLAEKDALMREIHHRVKNNLQIISSLLSMQQRALTDPASKAAVGDTRQRIAALALI
                     YRTLYQSDDLRYADACIFLTELVGQLVASEAGRGIVVTSSVEADSLVVDPDKLAPLAL
                     WLVEAVTNAQKHAFAGRGGDLQVRFQVRGDKSVLEVQDDGPGVSETFRAGVGRTLMGA
                     FAKQLRGEVEMVPAPGGGTIARLTFATPEAVVPTDPADVGTSSGPQR"
BASE COUNT          297 a          652 c          682 g          256 t
ORIGIN      
        1 cgcgcccagc ggccggacca cgaagcccag gccgaaggtc agcagggcca tgatgtaggc
       61 gaccgtctcg ccggcgtcgg cgaagaaatg ccgcgcgatg atggtggtca gggagccgaa
      121 gacgaagaaa tcataccatt cgaacgcggt ccccgccgcc gaggcggtga ccacagtctt
      181 caggccgggt cccgcccggg tctccgccgc atccgtcatc gatcgctccc cgcgtgtcgg
      241 tcccgcgtct ctgcggacgc ctgcgccgcg atgctagcgg gcgggacggg gcccagacaa
      301 gcgaaagccc gtcagcgggg ggatgctgac gggccttctt caaccgatcc gcgtgtcggg
      361 gggaagcgcg gtcgggatcg gggaaaccta gttcttggcg tcttccgcgg ccgtggtcac
      421 ggcgctgccg gcggccgagg cgtcgcgacc cacgcccgaa acggtattgc acgccgcggt
      481 cgtcagagcg gcggcggctg cgaacagtac aaacagtttg cgcatcagat gaactccata
      541 cgccggggag gcggcgcctg cagtatcaac gttgcggccc ggacgaagtt ccaacatcgg
      601 ccggatccgt cggcacgacg gcctcggggg tggcgaaagt caggcgcgcg atcgtgcctc
      661 cccccggagc aggaaccatc tcgacctcgc ccctgagctg tttggcgaac gcccccatca
      721 gggtgcgccc caccccggcg cggaaggtct cgctgacccc cggtccgtcg tcctggacct
      781 cgagcacgct cttgtcgccc cgcacctgga agcggacttg caggtcgccg ccgcggccgg
      841 cgaaggcgtg cttctgggcg ttggtcacgg cttcgaccag ccacagggcc agcggcgcca
      901 gcttgtcggg atcgaccacc agagagtccg cctcgaccga cgacgtcacc acgattccgc
      961 ggcccgcctc gctggcgacc aactggccga ccagttcggt caggaagatg catgcgtcgg
     1021 cgtagcgcag gtcgtcgctc tggtagaggg tgcggtagat cagcgccagg gccgcgatcc
     1081 gctggcgcgt gtcgcccacg gccgccttgg aggccggatc ggtcagggct cgctgctgca
     1141 tggacagcag cgaggagatg atctgcagat tgttcttcac ccggtgatgg atctcgcgca
     1201 tcagcgcgtc cttttccgcc agcgaggcgg tcagggccgc gtcgcgggtg acgatggact
     1261 cggccagttc gtccagcgtc cgggcaagaa cccggatttc cgcgggcgca ttcatggcct
     1321 gcagcgggcg cacattgaac cgcccgcggg cgtagatggc cgcgatccgc tccagatagt
     1381 tcagccagcg gatgacgatc cgctcgctga ccagcatcac ggcgccgaag gcgatgaacc
     1441 aggccagcag gggcatgagc agggccccga ccggattcag ccgcgcccag gacagcaggc
     1501 cgggcgcggg cgcggacagc agggcgtaga cgtcgcgccc ggccagggcg gcgccggcgt
     1561 agacgcggtg ccggccccgg gcgtcatccg cctcgaagat cgccgaggcg tcggagcggg
     1621 cgcggccgac ccagcccgtc aggctctggc ccccggacag gacgaaggcg tcggcctcgg
     1681 tcgccgtcag cagccgcccg tcgccgtcgg tgagggccgc ctgcgatcct tccggcaggg
     1741 cgggatcgtc cacgtcgggc tgcaggctgg cgaggggcag cagggccacc atggcgccgt
     1801 cgaaggcgcc cagcggtcgt tccagccgga tggccacgat caggccaggc gagcggcctg
     1861 cgacggtcgg cgcccgggcg aggacca
//
LOCUS       DASVVN010000365         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_119110,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000365 DASVVN010000000
VERSION     DASVVN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119110"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF686_05870"
     CDS             <1..261
                     /locus_tag="VF686_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8661374.1"
                     /translation="AIARALAKDPKILFADEPTSALDGENGQIVIRLLRRAATEHGAA
                     VICVTHDPRLEEWADRVIKIEDGVIIDDQRRIPNPDATLASH"
     gene            282..1307
                     /locus_tag="VF686_05875"
     CDS             282..1307
                     /locus_tag="VF686_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8661375.1"
                     /translation="MPAFLKNKWLWIGLVLIIVLVVGFTFMQSAGKAKKAEEEKANAQ
                     AKVESPYAAIANGKVDIEGGIIQIAARRGGVVRQVLVQEGDRVVAGQILARQEDDEPR
                     LALQSASADVAQAESQLRLIQVDIRTAQREHDRLQQLVASNFVAASRMDAARDAIAQA
                     QARLANQQAAVQTARARRDQAAYNVELTVIRAPQSGRIVRRYANPGAGASTLNVSNMF
                     DLEPDAPRIARAEIVESDIPNITPGQAVEITPEGDPSKVYVGAVLRRAAVFGGRKLQS
                     DDPTERTDERVVEVVVTADGAPLLIGQRVLVKFMKAGQRAGVKRVPAGPPAPERRAQL
                     TAARAAA"
     gene            1337..>1900
                     /locus_tag="VF686_05880"
     CDS             1337..>1900
                     /locus_tag="VF686_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661376.1"
                     /translation="MAGIRFDGRVVIVTGAGGGLGRAHALEFARRGAAVVVNDLGVHV
                     DGNGRSSAAADAVVAEITAAGGRAIADGASVTDDEGVQRLVAHTLAAFGRIDVLVANA
                     GILRDKTFAKMELADFAAVLDVHLLGTVKPLKAVWPLMRQQGYGRVVVTTSSSGLYGN
                     FGQANYGAAKLALVGLMNTLRLEGARDN"
BASE COUNT          297 a          723 c          629 g          251 t
ORIGIN      
        1 gcgatcgccc gcgccctcgc caaggatccc aagattctgt tcgccgacga gccgacctcg
       61 gcgctggacg gcgagaacgg ccagatcgtc atccgcctgc tgcgccgcgc cgccaccgaa
      121 cacggcgccg ccgtcatctg cgtgacccac gacccccgcc tcgaggaatg ggccgaccgg
      181 gtcatcaaga tcgaggacgg cgtgatcatc gacgaccaac gccgcattcc caatcccgac
      241 gccacgctgg cctcgcactg acgccttcaa ggactttcga catgcccgcc tttctcaaga
      301 acaaatggct ctggatcggt ctggtcctga tcatcgtgct cgttgtcggc ttcacgttca
      361 tgcagagcgc cggcaaggcg aagaaggccg aggaagagaa ggccaacgcc caggcgaagg
      421 tcgaaagccc ctatgcggcc atcgccaacg gcaaggtcga catcgagggc ggcatcatcc
      481 agatcgcggc ccgccgcggc ggcgtggtcc gtcaggtgct ggtgcaggaa ggcgaccgcg
      541 tcgtcgccgg ccagatcctc gcccgtcagg aggacgacga gccccgtctg gccctgcaga
      601 gcgccagcgc ggacgtcgcc caggccgaaa gccagctgcg cctgatccag gtcgacatcc
      661 gcaccgccca gcgcgagcac gaccgtctgc agcagctggt ggcctccaac ttcgtcgccg
      721 cctcccgcat ggacgccgcc cgcgacgcca tcgcccaggc ccaggcccgc ctcgccaacc
      781 agcaggccgc cgtccagacc gcccgcgccc gccgcgatca ggccgcctac aatgtcgagc
      841 tgacggtgat ccgtgcgccg cagtcgggcc ggatcgtgcg ccgctacgcc aaccccggcg
      901 ccggcgcctc gaccctgaac gtctccaaca tgttcgacct cgagcccgac gccccgcgca
      961 tcgcccgcgc cgagatcgtc gaaagcgaca tccccaacat cactcccgga caggccgtcg
     1021 agatcacccc cgagggcgat ccgtccaagg tctatgtcgg ggccgtgctg cgccgcgccg
     1081 ccgtcttcgg cggccgcaag ctgcagtcgg acgatcccac cgagcgcacc gacgagcggg
     1141 tggtcgaagt ggtggtcacc gccgacgggg ccccgctgct catcggccag cgtgtgctgg
     1201 tcaagttcat gaaggccggt cagcgcgcgg gcgtgaagcg cgtcccggcg ggcccgcctg
     1261 cccccgaacg gcgagcccag ttgaccgcag cccgcgccgc cgcctaaggc cgcccctcca
     1321 cgaggagggc gcgcgcttgg ccgggattcg ctttgacgga cgggtggtca tcgtcaccgg
     1381 ggccggcggg ggcctcggcc gcgcccatgc gctcgagttc gcccggcggg gcgccgcggt
     1441 ggtggtcaac gacctcggcg tgcacgtgga cggcaacggt cgttcctccg ccgccgccga
     1501 cgcggtggtc gccgagatca ccgccgccgg cggccgcgcc atcgccgacg gcgcctcggt
     1561 gaccgacgac gaaggcgtcc agcggctggt ggcgcacacg ctcgccgcct tcggccgcat
     1621 cgacgtgctg gtcgccaacg ccggcatcct gcgcgacaag accttcgcca agatggagct
     1681 ggcggacttc gccgcggtcc tcgacgtcca tctgctcggc acggtgaagc cgctcaaggc
     1741 ggtgtggccc ctgatgcgcc agcagggcta cggccgggtg gtggtcacca cctcctcgtc
     1801 cggcctctac ggcaacttcg gccaggcgaa ctacggcgcg gccaagctgg ccctggtcgg
     1861 gctgatgaac acgctgcggc tggagggcgc gcgcgacaac
//
LOCUS       DASVVN010000366         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_39402, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000366 DASVVN010000000
VERSION     DASVVN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39402"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF686_05885"
     CDS             complement(<1..242)
                     /locus_tag="VF686_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661377.1"
                     /translation="MTETYTGPTNVEDIEDDADTATATLDRQADEILAENRSFGSRPL
                     RQAIREDALQARDWGRTRTERLRHAVEEEPVKASLYA"
     gene            380..562
                     /locus_tag="VF686_05890"
     CDS             380..562
                     /locus_tag="VF686_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661378.1"
                     /translation="MTYIEPDSPGGQPEILPPDTPGPDYEPGGAPPEDPGGMPEEMPQ
                     QDIPGGDPGDSRPYGG"
     gene            676..900
                     /locus_tag="VF686_05895"
     CDS             676..900
                     /locus_tag="VF686_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX8661379.1"
                     /translation="MAQGQPTRASGVSKRGFASMDPERQREIARKGGASVPSEKRSFS
                     QDRSLAAQAGRKGGEASHGARVREPGTTAD"
     gene            complement(958..>2664)
                     /gene="egtD"
                     /locus_tag="VF686_05900"
     CDS             complement(958..>2664)
                     /gene="egtD"
                     /locus_tag="VF686_05900"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX8661380.1"
                     /translation="MDVLNLMSRSPLEPATYEAAPRNGGLEPPRGGWSGFDGGLVEVG
                     HGGAGFAFDNEGPAHRVWLEPYALANDLVTNGDWLAFIEDGGYARPELWLADGWAVVK
                     AEHWTAPLYWRADDGRWTRMSLTGRVDIDPAEPVRHVSFYEADAFARWSGKRLPTEGE
                     WEHAVRCRPDAFSNAFGEVWQWTASAYAPYAGFKPTEGTASEYNGKFMANQMVLRGSS
                     FATASGHARLTYRNFFYPHQRWAFLGLRLADDASAPLTRETEPGETARFRRDLIAGLS
                     RSPRVASPKWFYDAEGSRLFEEITRLPEYYPTRQEAALLRRAAPEWAARFGPGAVLVE
                     FGSGASEKTRIVLDAAPGLAAYVPIDISADALDAAARRIADAYPELKVAPLVGDFLHL
                     GTLPAGTGQGRRVGFFPGSTIGNLERDEAVAFLKAARSLLGEDALFILGVDLVKEPEV
                     LIAAYDDAAGVTAAFNRNLLVRANRELGADFDVDAFAHRAVWNPADSRMEMHLEATRD
                     MAVSLDGGTITFARGETIHTENSRKFTEEAVRDLAKAAGWSVAAFETGDAPAVALALL
                     EA"
BASE COUNT          434 a          950 c          886 g          394 t
ORIGIN      
        1 gcgtaaagtg acgccttgac tggctcttcc tcgaccgcgt gacgcaaccg ctccgtccgg
       61 gtccttcccc agtctcgcgc ctgcagggcg tcttcgcgga tggcctgccg caacggccgg
      121 gagccgaagc tgcggttctc ggccaggatt tcgtcggcct gacggtccag agtggccgtc
      181 gcggtgtcgg cgtcgtcctc gatatcctcg acgttggtcg ggccggtgta ggtctcggtc
      241 atgagggact cctgcggtgt tgggttgagc cggtcaaacc aatgtcgggc gacgcggttc
      301 cgggcaaaac gtcgcgctcc cgaaacgtga ttgaacagcg ctgcggtacg ggacgtttca
      361 ctctccgaag gagaccgcga tgacctatat cgagcccgac agccccggtg gccaacccga
      421 gatcttgccg cccgacactc cgggtccgga ctacgaaccg ggcggcgccc cgcccgagga
      481 tcccggcggc atgccagagg aaatgccgca gcaggatatc cccggcggcg accccggcga
      541 ctcccgtccc tacggcggct gaccacgcaa actctgtgat tcgttcgcaa ttctgtgacc
      601 ggacaaccgg aaccggaccg gggtggaagg gttcagttca gcaaccgcca ccgtcgccac
      661 gaaaggaacc ccccgatggc tcaaggtcag cccacccgcg cctccggcgt ctccaaacgg
      721 ggcttcgcct ctatggaccc cgaacgccag cgtgagatcg cccgcaaggg cggagccagc
      781 gtgcccagcg agaagcgaag cttctcccag gatcgcagcc tcgccgcaca ggcgggccgc
      841 aagggcggtg aagcgtctca tggggcgcgc gttcgagagc cgggcacgac cgcagattaa
      901 ggtccgccac cgcagaaatc gaagggcggc gtccgcgtgg acgccgccct tttcgtgtca
      961 ggcctcgagc agggccaagg cgacggccgg cgcatcgccc gtctcgaagg cggcgaccga
     1021 ccaacccgcg gccttggcca gatcgcgcac cgcctcttcg gtgaatttcc gcgagttctc
     1081 ggtgtggatg gtctcgcccc gggcgaaggt gatcgtcccg ccgtcgaggc tgaccgccat
     1141 gtcccgcgtc gcctccagat gcatttccat ccgggagtca gccgggttcc agaccgcgcg
     1201 atgggcgaag gcgtcgacat cgaagtccgc tcccagctca cgattcgccc ggaccagcag
     1261 attgcggttg aaggcggccg tcacccccgc cgcgtcgtca taggcggcga tcaggacttc
     1321 cggctccttg accagatcca cgccgaggat gaacagggcg tcttcgccaa gcagcgagcg
     1381 cgccgccttc aggaaggcga cggcctcgtc ccgctccaga ttgccgatgg tcgaacccgg
     1441 gaagaagccg acccggcggc cctgaccggt ccccgccggc aaggtcccca gatgcaggaa
     1501 gtcgccgacc agcggcgcca ccttcagttc gggataggcg tcggcgatcc ggcgcgcggc
     1561 cgcgtccaag gcgtcggcgc tgatgtcgat cggcacatag gcggcgaggc ccggcgccgc
     1621 atccagcacg atccgcgtct tctcgctggc cccggatccg aactcgacca gcacggcgcc
     1681 gggcccgaac cgcgccgccc actccggcgc cgcccggcgc agcagggccg cctcctgccg
     1741 ggtcggatag tattcgggca gccgggtgat ctcctcgaag aggcgcgagc cctcggcgtc
     1801 atagaaccac ttcggcgagg cgaccctggg cgaccgcgac aacccggcga tcaggtcgcg
     1861 gcggaagcgg gcggtctcgc ccggctcggt ctcccgcgtc aggggcgccg aagcgtcgtc
     1921 ggccagtcgc agtcccaaga acgcccacct ttgatgcgga tagaagaagt ttcgatacgt
     1981 gagccgcgcg tgtccggatg cagtggcgaa ggacgaaccc cgcagcacca tctgattggc
     2041 catgaacttg ccattgtact cggaggccgt gccctcggtc ggcttgaacc cggcgtaggg
     2101 ggcgtaggcg cttgcggtcc actgccacac ctcgccgaag gcgtttgaga aggcatcggg
     2161 gcggcatcgc accgcatgct cccactcccc ctcggtcggg agccgtttgc ccgaccagcg
     2221 ggcgaaggcg tcggcctcgt agaagctgac gtgccggacc ggttcggccg gatcgatgtc
     2281 gacgcggccg gtcaggctca tacgcgtcca gcggccgtcg tcggcccgcc agtacagggg
     2341 cgcggtccag tgttccgcct tcaccaccgc ccagccgtcg gccagccaca gctcgggccg
     2401 ggcgtagccg ccgtcctcga tgaaggcgag ccagtcgcca ttggtgacca gatcgttggc
     2461 cagggcgtag ggctcaagcc acacgcggtg cgcaggacct tcattgtcga aggcgaagcc
     2521 ggccccgccg tgtccgacct cgaccagacc gccgtcaaac ccggaccagc cgccgcgcgg
     2581 cggttccagc cccccgttcc ggggcgccgc ctcataggtc gccggctcca gcggcgaccg
     2641 cgacatcagg ttcagcacgt ccat
//
LOCUS       DASVVN010000367         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_487026,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000367 DASVVN010000000
VERSION     DASVVN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487026"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..2000
                     /gene="typA"
                     /locus_tag="VF686_05905"
     CDS             171..2000
                     /gene="typA"
                     /locus_tag="VF686_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX8661381.1"
                     /translation="MNLRNVAIIAHVDHGKTTLVDQLLAQSGVFRANEAQSERAMDSN
                     DQEKERGITILAKCTSVLWNGKAGETRVNIIDTPGHADFGGEVERILGMVDGCVLLVD
                     AEEGVMPQTKFVLTKALKMGLRPILCINKVDRAHADPDKVHNAAFDLFAAIGATDEQL
                     DFPHIYASGRAGWATLDLNQPSDNLAPLFDLIVDHVPPPKQVEAADKPFQILNVLIES
                     DPFLGRLLTGRIESGKAVPGMSIKALSRDGKVIEQGRITKVLAFRGLKRQPVDSAEAG
                     DIVAIAGMSKATVADTLCALEVTEALPAQPIDPPTISMTVSVNDSPLAGREGDKVQSR
                     VIRDRLLKEAEANVAIRVTETGGKDAFEVAGRGELQLGVLIENMRREGFELSISRPRV
                     VYQTGENGERLEPIEDVVIDVDDEYTGVVIEKLSARKAELKDMGPSGAGKTRLTLKSP
                     SRSLIGYQGEFLTDTRGSGVLNRVFSHYEPHKGAIEGRLKGVLVSNGDGETAAFALWN
                     LEDRGVMFVGGGEKTYQGMIIGENARWDDLDVNPMKAKQLNNIRAAGKDEAVRLTPPR
                     RPSLEQAIAYIEEDELVEVTPKSIRLRKAVLNPSFRKKRVREE"
     gene            complement(2046..2522)
                     /locus_tag="VF686_05910"
     CDS             complement(2046..2522)
                     /locus_tag="VF686_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661382.1"
                     /translation="MSCGVALAVSLLLSDPNVALAAAQPPAPVAAPASVAGEPLFADI
                     VARSGALKGVVQGWIDAGVMNQADFAAGPAFTGFKVQAAELAASDMQGHLILKERGTD
                     GDLKCILRGISEDMPKKIAAVEAAATPAERRVALDELAYLLNDNVEVITAPPQPEV"
     gene            2626..>3021
                     /locus_tag="VF686_05915"
     CDS             2626..>3021
                     /locus_tag="VF686_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX8661383.1"
                     /translation="MTARIQALNMLRAVARKDASGRIAHRFATDPGRMERMGVEAAGL
                     YLDLSKQSWSAEGFEAALDLARTCDVEGRRDALFGGEAVNNPAGRAVLHPALRASEGA
                     DFKALGEAVSAEVDETRQAMAAFAADIGSG"
BASE COUNT          530 a         1035 c         1014 g          442 t
ORIGIN      
        1 gccctctgat cgccgctttc cgggcgcata agcccggatg aacgccagtg cgtgcttccc
       61 gcgcactggc gtttccgcgt tcgcccctgt atgaggcgcg cgctccccgc accgtcgcca
      121 aaatctcgcg gcccgctctc gcacggccgc cttgcacgtc tgatagtcaa atgaacctgc
      181 gcaacgtcgc catcatcgcc cacgtcgacc acggcaagac cacgctggtg gaccagcttc
      241 tggcccagtc gggggtcttc cgagccaatg aggcccagtc cgaacgggcg atggactcca
      301 acgaccagga gaaggaacgc ggcatcacca tcctggccaa gtgcacgagc gtgctctgga
      361 acggtaaggc gggcgagacg cgcgtcaaca tcatcgacac cccgggccac gccgacttcg
      421 ggggcgaggt cgagcgcatc ctgggcatgg tggacggctg cgtcctgctg gtcgacgccg
      481 aagagggcgt catgccccag accaaattcg tgctgaccaa ggcgctgaag atgggcctgc
      541 gcccgatcct gtgcatcaac aaggtagacc gcgcccacgc cgacccggac aaggtccaca
      601 acgccgcctt cgacctgttc gccgccatcg gcgcgacgga cgagcagctg gacttcccgc
      661 acatctacgc ctcgggccgc gccggctggg ccacgctgga tctgaaccag ccgtccgaca
      721 acctggcgcc gctgttcgat ctgatcgtgg accacgtgcc gcctcccaaa caggtcgaag
      781 ccgccgacaa gccgttccag atcctgaacg tgctgatcga gagcgacccc ttcctgggcc
      841 gcctactgac cggccgcatc gagagcggca aggccgttcc cggcatgtcg atcaaggccc
      901 tgtcgcggga cggcaaggtc atcgaacagg gccgcatcac caaggtgctg gccttccgcg
      961 gcctgaagcg tcagccggtg gacagcgccg aggcgggcga catcgtcgcc atcgccggca
     1021 tgtccaaggc caccgtggcc gacaccctgt gcgcgctgga agtgaccgag gcgctgccgg
     1081 cccagccgat cgatcccccg accatctcca tgaccgtctc ggtcaacgac agcccgctgg
     1141 ccggccgcga aggcgacaag gtccagtcgc gcgtcatccg cgaccgcctg ctcaaggagg
     1201 ccgaagccaa cgtcgccatc cgcgtcaccg agacgggcgg caaggacgcc ttcgaggtcg
     1261 ccggccgcgg cgaactgcag ctgggcgtgc tgatcgagaa catgcgccgc gaaggctttg
     1321 agctgtcgat cagccgtccg cgcgtggttt atcagaccgg cgagaacggc gagcgtctgg
     1381 agccgatcga ggacgtcgtc atcgacgtcg acgacgaata caccggcgtg gtcatcgaga
     1441 agctgtcggc ccgcaaggcc gagctgaagg acatgggccc gtcgggcgcc ggcaagacgc
     1501 gtctgacgct gaagtcgccg tcgcgctcgc tgatcggtta ccaaggcgaa ttcctcaccg
     1561 acacccgcgg ttcgggcgtg ctgaaccgcg tgttcagcca ctatgagccg cacaagggcg
     1621 ccatcgaagg ccgtctgaag ggcgtgctgg tctcgaacgg cgacggcgaa accgccgcct
     1681 tcgccctgtg gaacctcgag gaccgcggcg tcatgttcgt cggcggcggc gagaagacct
     1741 atcagggcat gatcatcggc gagaacgccc gttgggacga cctggacgtc aacccgatga
     1801 aggccaagca gctgaacaac atccgcgccg ccggcaagga cgaggctgtt cgcctgaccc
     1861 cgccgcgccg tccttcgttg gagcaggcca tcgcctacat cgaggaagat gagctggtgg
     1921 aagtgacgcc caagtcgatc cgcctgcgca aggccgtgct gaacccctcg ttccgcaaga
     1981 agcgcgtccg cgaggaatag gacgggcggg gcttcggccc cgtctcccag cctcggtccg
     2041 ggtcgtcaga cctcgggctg cggcggagcg gtgatgacct cgacgttgtc gttcagcaga
     2101 taggccagtt cgtccagcgc cacgcggcgc tcggcaggcg tggcggcggc ttcgacggcc
     2161 gcgatcttct tcggcatgtc ttccgagatg ccgcgcagga tgcatttcag atcgccgtcg
     2221 gtgccgcgtt ccttgaggat caggtggccc tgcatgtcgc tggcggcgag ttcggcggcc
     2281 tgaaccttga aaccagtgaa ggctggcccg gcggcgaagt cggcctggtt catcacgcca
     2341 gcgtcgatcc agccctgaac cacgcccttc aacgcgcccg agcgggccac gatgtcggcg
     2401 aacaggggct cgccggcgac cgaggcgggg gcggcgaccg gagccggcgg ctgggccgcg
     2461 gccagcgcca cattgggatc ggagagaagc agtgaaaccg ccagggcgac gccgcaggac
     2521 atggcgaacc tcttaaatct gggccgaatc acggattcag cgttgaaccg gcttacgcca
     2581 cacccgtaaa cggggactta ccccgccgct ctgcgagtcc cgcccatgac cgcccgcatc
     2641 caggctttga acatgctccg cgccgtggcg cgcaaggacg cgagcgggcg catcgcgcac
     2701 cggttcgcca ccgatcccgg ccgtatggag cgtatgggag tcgaggcggc gggcctgtat
     2761 ctggacctgt cgaagcaatc ctggagcgcc gaagggttcg aggccgcgct cgatctggcg
     2821 cgcacctgcg acgtcgaagg ccgccgcgac gccctgtttg gcggcgaggc ggtcaacaac
     2881 cccgcggggc gcgccgtgct gcacccggcc ctgcgggcga gcgagggcgc ggacttcaag
     2941 gcgctgggcg aggcggtttc ggccgaagtc gacgagacgc ggcaggcgat ggccgcgttc
     3001 gccgccgata tcggttcagg g
//
LOCUS       DASVVN010000368         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_487041,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000368 DASVVN010000000
VERSION     DASVVN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487041"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..751
                     /locus_tag="VF686_05920"
     CDS             101..751
                     /locus_tag="VF686_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX8661384.1"
                     /translation="MTPIVDPAIANPETVLGALDFAGVAVFAATGALAAARQRHDLVT
                     FAFFGAITGVGGGTLRDLLIGAPVFWVQDWRYIAVCLFASVVVWMLGARTWRFRALLW
                     LDALGLAAYGVMGAAKAEAYEVAPLICIVMGALTACFGGVVRDLLAGEPSILLRREIT
                     VSAALLAATVFVVARFAGLDAWPAALIAAPCGFALRAGALVWGWSLPAFPGTPSRG"
     gene            885..1445
                     /locus_tag="VF686_05925"
     CDS             885..1445
                     /locus_tag="VF686_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8661385.1"
                     /translation="MQVLSRPPSGWPALVLNADFRPLSYYPLSIWPWEEVVKAVYQDR
                     VDVVSTYDKVVRSPSMEIVLPSVIALKNYVDQDRQPAFTRFNVFLRDGFTCQYCHATE
                     QLTFDHVVPRSRGGRTTWENIVAACSPCNLRKGGRTPQQAGMPLRRAPHRPNAWQLQE
                     FGRRFPPHYLHQSWLDYLYWDIELEP"
BASE COUNT          193 a          518 c          530 g          283 t
ORIGIN      
        1 agatgtggaa gccgcaccgc agcgttgcgg cgcatctgct ttggggatgg tatggggcgg
       61 tcaagcgcgg cgaggtcgtg ctggaggaag gccctgtttc gtgactccca tcgtcgaccc
      121 cgccatcgcc aatcccgaga ccgttctggg cgcgctggat ttcgcgggcg tggcggtgtt
      181 tgcggccacc ggcgcgctgg cggcggcgcg gcagcggcat gacctggtca ccttcgcctt
      241 cttcggcgcg atcaccggcg tcggcggcgg cacgctgagg gatctgctga tcggcgcgcc
      301 ggtcttctgg gtgcaggact ggcgctacat cgccgtctgt ctgttcgcct cggtcgtggt
      361 gtggatgctg ggcgcgcgga cgtggcggtt ccgggcgctg ttgtggctcg atgcgctcgg
      421 ccttgccgcc tatggcgtga tgggggccgc caaggccgag gcctatgagg tcgcgccgct
      481 gatctgcatc gtcatgggcg cgctgacggc ctgttttggc ggggtggtgc gcgacctgct
      541 ggccggcgag ccctccatcc tgctgaggcg cgaaatcacc gtctccgccg ccctgctggc
      601 cgcgacggtc ttcgtcgtcg cacgcttcgc cgggctggac gcctggccgg cggccctgat
      661 cgccgcgccc tgcggcttcg ccctgcgcgc cggcgccctg gtctggggct ggagtctgcc
      721 cgccttcccc gggaccccct cgcggggctg agcgttacgg aaacgtcgcc gtgcggtcat
      781 cgccaagccg tcgaatcacg gctaggcttc aagcacagac aaggaaggag accggtcttc
      841 agtccgttcg cgtccatgtt cctctgctca aaggcgaggg cctgatgcag gtcctgagcc
      901 gccctccgtc cggctggccc gcactggtgc tgaacgccga cttccgcccc ctgtcctact
      961 atcccctgtc gatctggccg tgggaggaag tggtcaaggc ggtctaccag gaccgggtcg
     1021 acgtggtctc cacctacgac aaggtggtgc gcagcccgtc gatggagatc gtcctgccca
     1081 gcgtgatcgc gctgaagaac tatgtcgatc aggaccggca gccggccttc acccgtttca
     1141 acgtcttcct gcgcgatggc ttcacctgcc agtactgcca tgccaccgag cagctgacgt
     1201 tcgatcacgt cgtgccgcgg tcgcggggcg ggcgcaccac ctgggaaaac atcgtcgccg
     1261 cctgttcgcc gtgcaatctg cgcaagggcg gccgcacgcc gcagcaggcg ggcatgcctc
     1321 tgcgccgcgc gccgcatcgg ccgaatgcgt ggcagctgca ggagttcgga cggcgcttcc
     1381 cgccccacta tctgcaccag agctggctcg actatctgta ctgggacatc gagctggagc
     1441 cctgacgcgc gaaagcccgc gccgatggcg cgggcctcac gtcttccggt gaccctagcg
     1501 gcggcggcgg ttgaactggc tttc
//
LOCUS       DASVVN010000369         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_198771,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000369 DASVVN010000000
VERSION     DASVVN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198771"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VF686_05930"
     CDS             complement(<1..422)
                     /locus_tag="VF686_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661386.1"
                     /translation="MLIEMLAAMAVVSGGAGPAGQDHGTRVAPRGDYRESCSGEYTNR
                     GRLYADCRTNRGQIRGSSIELNRCSDYEIRNNDGLLVCGPNRGQYEDRPGGGGGGGNG
                     PGWPGGGGGRGSAMVYRDADFRGASFRFDDEVPNLANTG"
     gene            639..1310
                     /locus_tag="VF686_05935"
     CDS             639..1310
                     /locus_tag="VF686_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX8661387.1"
                     /translation="MTDILMPKATAVWLVDNTSLTFEQIADFCGLHPLEVRGIADGDV
                     ARDIRGADPLVNGQLTREELDKAQNDENYRMQAVVSRHAELMKGGKPAPKYTPVSRRQ
                     DRPDAIAWFVRNHPEVTDAQIAKMLGTTKSTIESVRNRTHWNSANIKPVDPVTLGLVG
                     QLALDELVRKAADKKAKDDAKKGGATLQPVDAPAEPAEEEFVPEARERRGAEPTAASV
                     FGTKD"
     gene            complement(1372..1545)
                     /locus_tag="VF686_05940"
     CDS             complement(1372..1545)
                     /locus_tag="VF686_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8661388.1"
                     /translation="MTIEARIRELGNRHRNLDETIRREMIHPATDSLHVRELKQQKLR
                     LKEQITSLEARAG"
     gene            1674..2288
                     /locus_tag="VF686_05945"
     CDS             1674..2288
                     /locus_tag="VF686_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX8661389.1"
                     /translation="MNGAVRNLSGATPPRLIVGISGASGVTYGVRTLDALRELGVESH
                     LVVTRAALLTLSQETDISADDLMSRADVTHRLNDVGASIASGSFRTMGMIVAPCSVRT
                     MSEIATGVTSTLLTRAADVVLKERRPLVLMVRETPLHLGHLRTMTALAEMGAAIAPPL
                     PAFYAKPTSIADIVDQSVGRALDVFGLDWSAVRRWDGLKSAAAE"
     gene            2285..2749
                     /locus_tag="VF686_05950"
     CDS             2285..2749
                     /locus_tag="VF686_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HEX8661390.1"
                     /translation="MSLWDWALTAYAAEGVAEACLELQDSAGQNIPLLLWAAWCAAEG
                     RTPDEDALEAAGDTARAWQETAIAPLRAVRRALKPRAPDLDDDAREAVRAQVKAVELE
                     AERRLLTALEALAPPPTGHAQPPLASMVAAARVWSPMTPRAGLVRLAERLPA"
BASE COUNT          446 a         1003 c          880 g          433 t
ORIGIN      
        1 ccggtgttgg ccaggttcgg aacctcgtcg tcgaaccgga agctcgcgcc gcggaagtcg
       61 gcgtcgcggt agaccatggc cgagccacgc cctccgccgc cgccaggcca gccgggaccg
      121 ttgccaccgc cgccgccgcc accgggccgg tcctcgtact ggccccgatt cggcccgcac
      181 accagcaggc cgtcattgtt gcggatttcg tagtcagaac accggttcag ctcgatggac
      241 gagccacgga tctggccgcg attcgtgcgg cagtcggcgt acagccgccc gcgattggtg
      301 tattcgccgc tgcagctctc gcgatagtcg ccgcgcggcg cgacccgcgt tccatggtcc
      361 tgaccggccg gaccagcccc gcctgaaacg accgccatcg cggccagcat ctcgatcaac
      421 atggtcgtcc ctccacaagg tttgcatgac gcacaacgcg tccgaggccc cactgttcag
      481 cttcccggat gaaccttggc tgtacgaggg cgccagcatc cgttttcttt tctgccgaat
      541 aggcctatat cgccatcatc aacgcccggt cgaaaggccg ggcgccgtcg tatccagagc
      601 ccggtttccg ggcctgaaaa ccaaaccgga atgcgttcat gaccgacatc ctcatgccca
      661 aggccaccgc ggtctggctg gtcgacaaca cctccctgac gttcgagcag atcgccgact
      721 tctgcggcct gcacccgctg gaagtgcgcg gtatcgccga cggcgacgtg gcccgcgaca
      781 tccgcggcgc cgatccgctg gtcaacggcc agctgacgcg cgaggaactg gacaaggccc
      841 agaacgacga gaactaccgc atgcaggccg tggtcagccg ccacgccgaa ctgatgaagg
      901 gcggcaagcc ggctccgaaa tacacccccg tctcgcgccg tcaggaccgc ccggacgcca
      961 tcgcctggtt cgtgcgcaac caccccgagg tgaccgacgc ccagatcgcc aagatgctcg
     1021 gcaccaccaa gtcgaccatc gagagcgtgc gcaaccgcac tcactggaac agcgccaaca
     1081 tcaagccggt cgacccggtg accctgggcc tcgtcggcca gctggccctg gacgaactgg
     1141 ttcgcaaggc cgccgacaag aaggcgaaag acgacgccaa gaagggcggc gccacgctcc
     1201 agcccgtcga cgcccccgcc gaaccggccg aggaggagtt cgtccccgag gcccgcgagc
     1261 gccgcggcgc ggagcccacc gccgcttcgg tgttcggcac caaggactag gataaaaacg
     1321 gccccggaga tcgctctccg gggccgatgt ctttctcggg aggcggcctc gctatcctgc
     1381 gcgggcctcc aggctggtga tctgttcctt aagcctgagc ttctgttgct tgagctctct
     1441 gacgtgaagc gagtccgtgg ccgggtggat catctctctc cgaatggtct catccagatt
     1501 gcggtgacgg tttcccagtt cgcggatacg agcttcgatg gtcatggctt gcgcctccat
     1561 gtctaaggga ccagtacagt cagcgccggg tctgacccgc tgtagaatca cattccggtg
     1621 aaacgccgac cgtcctgata cggaccaccc tggagcccga attggccgaa gatgtgaacg
     1681 gagccgtacg gaacctttcc ggcgcgaccc cgcccagatt gatcgtggga atttccggag
     1741 cgtccggcgt gacttacggc gtccggacgc ttgacgccct gcgcgaactg ggggtcgaaa
     1801 gccatctggt cgtgacccgc gccgccctgc tgaccctgtc gcaggagacc gatatttccg
     1861 ccgacgattt gatgagccgc gccgacgtga cgcaccggct caacgatgtc ggcgcctcca
     1921 tcgcctcggg ctcgttccgg accatgggga tgatcgtcgc cccctgctcg gtccgcacca
     1981 tgagcgagat cgccaccggc gtgacctcga ccctgctgac ccgcgccgcc gacgtggtgc
     2041 tgaaggagcg ccggccactg gtgctgatgg tgcgcgagac cccgctgcat ctcggccacc
     2101 tgcgcaccat gaccgccttg gccgagatgg gcgcggccat cgccccgccc ctgcccgcct
     2161 tctatgcgaa gccgaccagt atcgccgaca tcgtcgatca gtctgtcggg cgggcgctgg
     2221 acgtgttcgg cctcgactgg tcggccgtcc gccgctggga tggactgaag agcgcggccg
     2281 ccgaatgagt ctctgggact gggcgctcac ggcctatgcc gcggagggcg tggctgaggc
     2341 ctgtctggag cttcaggact ccgccggcca gaacatcccc ctgctgctct gggccgcatg
     2401 gtgcgccgcc gagggacgca cgccggacga ggacgccctc gaagccgccg gcgacaccgc
     2461 ccgcgcctgg caggaaaccg ctatcgcccc cctgcgcgcc gtgcgccggg ccctgaagcc
     2521 ccgcgcccct gatctggacg acgacgcccg cgaagccgtc cgcgcccagg tcaaggcggt
     2581 ggagctggag gcggagcgcc ggcttctgac agcgcttgag gcgctggccc cgccgccgac
     2641 tggccacgcg cagcctccgc tcgcctccat ggtcgccgcc gcccgggtgt ggtccccgat
     2701 gacgccgcgc gccgggctgg ttcgcctcgc cgagcgcctt ccggcctgat gcggcgcacg
     2761 gt
//
LOCUS       DASVVN010000370         6064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_14462, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000370 DASVVN010000000
VERSION     DASVVN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14462"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VF686_05955"
     CDS             <1..643
                     /locus_tag="VF686_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661391.1"
                     /translation="RFVEDEIRPLVALSLGHSLEAVGSTRRLAGKILSNDPRDRMEAL
                     RETLRLGEDEYEEGVFCWKGFLYYKWVLSSLTADIAAVSDAVSAVRPVGKLDQASREY
                     LDRGRSVLRTRIHKTCHDVALTLKIYDDAYAGLTVRGRPTAFRDFLLDAPQMFTRLGD
                     QLGAIQHIVSFWRFRFSPGASNVSPEELIDIFMDFETGLADRDQEPGQLVLAA"
     gene            complement(706..2097)
                     /gene="ahcY"
                     /locus_tag="VF686_05960"
     CDS             complement(706..2097)
                     /gene="ahcY"
                     /locus_tag="VF686_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8661392.1"
                     /translation="MTDYIVRDISLAPFGNKEIAIAETEMPGLMALREEFGGKQILKG
                     ARIAGSLHMTIQTAVLIQTLETLGAEVRWASCNIFSTQDHAAAAIAANGTPVFATKGE
                     TLEEYWDYAHKIFEWADGGYPNLILDDGGDATLLCVLGPKAEKDLSLLANPQNEEEEA
                     LFKVMKRYIAEKPGFYSAIRDAIGGVSEETTTGVHRLYEMAKKGDLPFPAINVNDSVT
                     KSKFDNLYGCRESLVDAIRRGTDVMLSGKVAVVCGYGDVGKGSAASLRQGGARVKVTE
                     VDPICALQAAMEGYEVVTVEDVAGEADIFVTATGNKDVLTVEHMRAMKNNAIVCNIGH
                     FDSEIQVAGLKNFKWDEIKPQVHHVEFPDGKKIILLSEGRLVNLGNATGHPSFVMSAS
                     FTNQTLAQIELWTNAKSYENQVYTLPKHLDEKVAMLHLGKLGAKLTTLSKEQADYISV
                     PTEGPFKPDHYRY"
     gene            2241..3164
                     /locus_tag="VF686_05965"
     CDS             2241..3164
                     /locus_tag="VF686_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX8661393.1"
                     /translation="MSGLKLTRRTMAAAGLFSLAAPTLLRAQDPPSFSTGDQRQGLLT
                     VPVTINDRGPYTFAIDSAANTSVIARDLATSLELSDAGEVTMHTLVAGEQAPTVRADR
                     VRTGALNASDVRLVVTGRPGLLGLDGLLGTDLLAGLRLVLHFGRRTRIDITRPRRWGD
                     NAGDGQRLSARSASAGEQRFNGLLMVDATARTVPCKVIIDTGARMTVINTALARLAGA
                     QPLILPDGTTVSRVLSPTGRGAVAEAMLLPELRLGGISLTRVPVLVGDFHTFAIWGLA
                     DQPAALLGVDLLGLFQTVIIDLKRGELVLDP"
     gene            3475..3663
                     /locus_tag="VF686_05970"
     CDS             3475..3663
                     /locus_tag="VF686_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8661394.1"
                     /translation="MYIWSATVNAADLLGLSTEIGTLEPGKRAAIVAVDGDPLTDVTV
                     LTSMDFVMRDGRVYRDGE"
     gene            3706..4701
                     /gene="bioB"
                     /locus_tag="VF686_05975"
     CDS             3706..4701
                     /gene="bioB"
                     /locus_tag="VF686_05975"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX8661395.1"
                     /translation="MTQDLATPRHDWTLNEVEALFARPFMDLVYEAATVHRTWFDPSE
                     VQKSQLLSIKTGGCAENCGYCSQSASFQTGLKAEKLMDTSAVLAEAMAAKAGGASRFC
                     MGAAWRELKDRDTPKLATMIAGVKALGLETCATLGMLTADQARQLKNAGLDYYNHNLD
                     TGPEYYAQVVTTRTYQDRLDTLQHVRDAGMATCCGGIVGMGEARRDRASLLHALATLP
                     EHPDSLPVNALVPVTGTPLGERMLAEGQIDPIEFVHTVAVARIVCPKAMVRLSAGRET
                     MSAEMQALCFLAGANSIFVGGKLLTTPNPEQDEDVALFALLDLKPMEPRAVEAAA"
     gene            4736..5110
                     /locus_tag="VF686_05980"
     CDS             4736..5110
                     /locus_tag="VF686_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HEX8661396.1"
                     /translation="MERRALLGLKSQSLKTSGGSSAPQASYLRLPTITANVIRPGGRR
                     GVMTVETGIDTADAALRTRVAQSAPRLRAAYAVVVQQAAAALLPGAPPDVERLVAQLQ
                     AATNATMGRAGARLLIGTVMVV"
     gene            complement(5412..>6064)
                     /locus_tag="VF686_05985"
     CDS             complement(5412..>6064)
                     /locus_tag="VF686_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8661397.1"
                     /translation="ESLEMQAGFYGVPKGERRTDELLAALGLSDKSDAYVRSLSGGMK
                     RRLMVAKALVHNPPILILDEPTAGVDVELRRQLWDYVRRINAEGVTVLLTTHYLEEAQ
                     ELCDTIAIMNRGEVVACEPTPQLLRRLDTRNVVVTPEGELNGLPELRGFETVARGNGA
                     FVVTYRKGQSSVEQVLAAVRAAGVTIADITTEDPDLEDVFLALTYGDANQVSPTKD"
BASE COUNT          962 a         1994 c         2034 g         1074 t
ORIGIN      
        1 ccgtttcgtc gaggacgaga tccgtccgct ggtcgccctg agcctggggc acagcctcga
       61 ggccgtgggt tcgacccgcc gtctggcggg gaaaatcctg tcgaacgatc caagggaccg
      121 gatggaggcc ctgcgcgaaa ccctgcgtct gggcgaggac gaatacgagg aaggcgtctt
      181 ctgctggaag ggtttcctct attacaaatg ggtgctcagc agcctgacgg cggacatcgc
      241 cgccgtctcg gacgcggtct ccgcggtccg gcccgtgggc aagctggatc aggcctcgcg
      301 cgaatatctc gaccgcggcc gctccgtgct ccggacccgc atccacaaga cctgtcacga
      361 cgtcgccctg acgctgaaga tctacgatga cgcctacgcc ggcctgaccg tcaggggccg
      421 tcccaccgcc ttccgcgact tcctgctgga cgcaccgcag atgttcaccc ggctgggcga
      481 tcagctcggg gccatccagc atatcgtcag cttctggcgc ttccgcttca gccccggcgc
      541 ctcgaacgtc agccccgaag agctgatcga catcttcatg gatttcgaaa ccggtctcgc
      601 cgaccgcgat caggaaccgg gccagctcgt cctcgcggcc tgaggtgtga aaaaggccgg
      661 gaagcaaact ccccggcctc cttcagtttc gatcgtcgct gccgatcaat agcggtagtg
      721 gtccggcttg aacggccctt cggtcgggac cgagatatag tcggcctgtt ccttcgacag
      781 cgtcgtcagc ttcgcgccca gcttgcccag gtgcagcatg gcgaccttct cgtcgaggtg
      841 tttgggcagg gtgtagacct ggttctcgta ggacttggcg ttggtccaca gctcgatctg
      901 ggccagcgtc tgattggtaa agctggcgct catgacgaac gaggggtggc ccgtggcgtt
      961 gcccaggttc actaggcggc cttccgacag caggatgatc ttcttgccgt ccgggaattc
     1021 gacgtgatgc acctgcggct tgatctcgtc ccacttgaag ttcttcaggc ccgcgacctg
     1081 aatctcggaa tcgaagtggc cgatgttgca gacgatggcg ttgttcttca tcgcccgcat
     1141 gtgctcgacg gtcaggacgt ccttgttgcc cgtggcggtg acgaagatgt cggcctcgcc
     1201 cgccacgtcc tcgacggtga cgacctcata gccttccatc gcggcctgca gggcgcagat
     1261 cggatcgacc tcggtgacct tcacccgggc gccgccctgg cgcagcgaag cagccgagcc
     1321 cttgcccacg tcgccatagc cgcagaccac ggcgaccttg cccgacagca tgacgtcggt
     1381 gccgcggcgg atcgcgtcga ccagcgattc acggcagccg tacaggttgt cgaacttgga
     1441 cttggtgacg ctgtcgttga cgttgatggc ggggaacggc aggtcgccct tcttggccat
     1501 ttcgtacagg cggtgaacgc cagtggtggt ctcttccgac acgccgccaa tggcgtcgcg
     1561 gatggccgaa tagaagcccg gcttctcggc gatgtagcgc ttcatgacct tgaagagggc
     1621 ttcctcttcc tcgttctgcg gattggccag cagcgacagg tccttctcgg ccttggggcc
     1681 gagtacgcac agcagggtgg cgtcgccgcc gtcgtcgagg atcaggttcg gatagccgcc
     1741 gtcggcccat tcgaagatct tgtgggcgta gtcccagtac tcttccagcg tctcgccctt
     1801 ggtggcgaac accggggtgc cgttggcggc gattgccgcc gcggcgtggt cctgggtcga
     1861 gaagatgttg cacgacgccc agcggacctc ggcgcccagc gtttccagcg tctggatcag
     1921 cacggcggtc tggatcgtca tgtgcaggct gccggcgatg cgcgcgccct tcaggatctg
     1981 cttgccgccg aactcttcgc gcagggccat caggccgggc atttcggttt cagcgatggc
     2041 gatttccttg ttgccgaacg gggcaagcga gatgtcgcgg acgatatagt cggtcatggg
     2101 ggccttccgg ggagactgtt cgggcggcct atagaccggt agcggcccgg gagcaagaaa
     2161 cacataagga tgtctttatg tgtttccggt gaaagcccgg ccccgacgtt tcgtgccacc
     2221 gtggtggaaa ggagcgaaac atgtctggcc tgaagctgac gcgccgcacg atggccgccg
     2281 ccggactgtt tagcctggcc gcgccaacgc tgttgcgggc gcaggacccg ccgtcgttct
     2341 cgaccggcga ccagcgacag ggactgctga ccgtccccgt gaccatcaat gatcgcggtc
     2401 cctacacctt cgccatcgac agcgccgcca acacctcggt catcgcccgg gacctcgcca
     2461 ccagcctcga actgtccgat gcgggagagg tgacgatgca taccctggtc gctggcgaac
     2521 aagcgccgac ggtgcgggcg gaccgggtgc ggaccggggc gctgaacgcc agcgacgtcc
     2581 ggctggtggt cacgggccgg ccgggcctgc tgggattgga tggcctgctg ggcacggacc
     2641 tgctggccgg tctcagactt gtgctccatt tcgggagacg gacccggatc gacatcacgc
     2701 gtccgcgacg atggggcgac aatgccggcg acggtcagcg actgagtgct cggagcgctt
     2761 cggcgggcga gcagcgcttc aacggcctgc tgatggtcga cgccacggcc cggacggttc
     2821 cgtgcaaggt gatcatcgac accggggcca ggatgaccgt catcaacacc gcgctcgccc
     2881 gtctggccgg cgcgcagcct ctgatcctgc ccgacggcac gaccgtaagc cgggtcctgt
     2941 cgccgaccgg ccggggcgcg gtggcggagg cgatgctgct gcccgagctt cggctgggcg
     3001 ggatcagtct gacccgcgtt ccggttcttg tgggggattt ccacaccttc gcgatctggg
     3061 gtctggccga tcagccggcc gcccttctgg gggtggatct gctggggctg ttccagaccg
     3121 tgatcatcga cctgaagcgg ggcgagctgg ttctggaccc gtgaggcgcg ctggtgtcta
     3181 tctggcggtc gtgttggagg ggtttttatg caggcgcctt ggcggcgctg acgatgggcg
     3241 gcgcaagcgc ggcccaggaa gtagtttcgg cgccggctgg tccggtcacc ctgatccacg
     3301 ccggccgact gctggaccgc cccggtcagg ccccgcgagg cccctcgacc gtggtggtgc
     3361 gcgacgggcg gatggtcgag atccgcgacg gcttcgtcga cgcgtcggcc ttccccggcg
     3421 ccacggtcat cgaccagcgc gaccgtttcg tcctgcccgg cctgatcgac agccatgtac
     3481 atctggtccg cgaccgtcaa cgccgccgac ctgctgggcc tgtcgaccga gatcggcacg
     3541 ctggagccgg gcaagcgggc ggccatcgtc gccgtcgacg gcgatccgct gacggacgtg
     3601 accgtcctga cctcgatgga tttcgtcatg cgcgacggcc gcgtctaccg cgacggggag
     3661 taaggcgcct tggcgagggc gtggggcgga gctaagcagg cgcgcatgac ccaggacctc
     3721 gccacccctc gccacgactg gacgctgaat gaggtcgagg ccctgttcgc ccggccgttc
     3781 atggatctgg tctatgaggc cgccaccgtg caccggacat ggttcgatcc gtccgaggtg
     3841 cagaagagcc agctgttgtc gatcaagacc ggcggctgcg ccgagaactg cggctactgc
     3901 tcccagtcgg ccagtttcca gaccggtctg aaggccgaaa agctgatgga tacgagcgcc
     3961 gtcctggccg aggccatggc ggccaaggcc ggcggggcca gcaggttctg catgggcgcg
     4021 gcctggcggg aactgaagga tcgggacacg ccgaaactgg cgaccatgat cgcgggggtg
     4081 aaggctctgg gtctggagac ctgcgccacc ctaggcatgc tgaccgccga tcaggcgcgc
     4141 cagctcaaga acgccgggct ggattattac aatcacaatc tggatacggg cccggagtac
     4201 tacgcccagg tggtgaccac ccggacctat caggatcggt tggacacgct gcagcacgtc
     4261 cgcgacgcgg gtatggcgac ctgctgcggc ggcatcgtcg gcatgggcga ggcgcggcgc
     4321 gaccgggcca gcctgctgca tgcgctggcg actctgccgg agcatcccga cagcctgccg
     4381 gtgaatgcgc tggtgccggt cacgggcacg ccgctgggcg agcggatgct ggccgagggc
     4441 cagatcgatc cgatcgaatt cgtccacacc gtcgccgtgg cccgcatcgt ctgcccgaag
     4501 gcgatggtgc gtctgtccgc cggccgcgag acgatgagcg ccgagatgca ggcgctgtgc
     4561 ttcctcgccg gcgccaattc catctttgtc ggcggcaagc tgctgaccac gcccaatccc
     4621 gaacaggacg aggatgtggc cctgttcgcc ctgctggatc tgaagccgat ggagcccagg
     4681 gcggtagagg cggcggcgta accacgcctt aagcagcgtg tgtcagggtc agcgcatgga
     4741 acgccgcgcc ctcctcggac tgaagtcaca gagcctgaag accagtggcg gctcgagcgc
     4801 gccccaggcc agctatctgc gcctgccgac tatcaccgcc aacgtcatcc gccccggcgg
     4861 tcggcgcggc gtgatgacgg tcgaaaccgg aatcgacaca gccgacgccg cgctgcggac
     4921 ccgcgtcgcc cagtcagccc cccgtctgcg cgccgcctac gccgtcgtgg tgcaacaggc
     4981 cgccgcggcc ctgctgcccg gcgctccgcc tgatgtcgaa cgtctggtcg cccagcttca
     5041 ggcggcgacc aacgccacca tgggccgtgc cggggcgcgc ctgctcatcg gcacggtgat
     5101 ggtggtctag gttcagcctg accgtcgtcc gtggcacggt tttggaattc ggggcgccaa
     5161 ccgtgccaat cgcgccattc atgggcgtgg aaatcgtttc aaaatcaatc ggctggcaat
     5221 tcagccgaag ttgagccgcg ccagattatt agaaattttc gcagcaaacc gccgctgaag
     5281 ccgccgcgtt cgtcgttcga ttgtcaaaga ccgcacgcta tcgaaatggg accaggctgg
     5341 cggcggtcaa gatgggtgag gaataaattc ctgcccgact cctcgcgcag ggcgcctgaa
     5401 tggcgatgac ctcagtcttt cgtcggactg acctgattgg cgtctccata ggtcagcgcg
     5461 aggaagacgt cttccagatc cggatcctcg gtggtgatgt cggcgatcgt cacgcccgcc
     5521 gcacgcacgg ccgccagcac ctgttccacc gacgactggc ccttgcggta ggtgaccacg
     5581 aaggcgccgt ttccacgcgc cacggtctcg aaccccctga gttcgggcag gccgttcaac
     5641 tcgccctcgg gcgtgaccac gacgttgcga gtgtccagcc ggcgcagcag ctgcggcgtc
     5701 ggctcgcagg ccaccacctc gccccggttc atgatggcga tggtgtcgca gagctcctgc
     5761 gcctcctcga gatagtgggt ggtcagcagc accgtcacgc cctcggcgtt gatgcgccgg
     5821 acgtagtccc acagctggcg gcgcagctcg acgtccacgc cggcggtcgg ctcgtcgagg
     5881 atcaggatcg gcggattgtg aaccagcgcc ttggcgacca tcagccggcg cttcatgccc
     5941 ccggacagcg aacggacgta ggcgtcggac ttgtcgctga gccccagcgc cgccagcagc
     6001 tcgtccgtgc ggcgctcgcc cttgggaacg ccgtagaagc ccgcctgcat ctccagactt
     6061 tcgc
//
LOCUS       DASVVN010000371         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_119198,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000371 DASVVN010000000
VERSION     DASVVN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119198"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VF686_05990"
     CDS             complement(<1..886)
                     /locus_tag="VF686_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8661398.1"
                     /translation="MTAFASAVGQAASAQTRDPAAVAFYDRFNDQLATLPAGMQPDVR
                     AFYELNGWRPVWTAERLRALQAAAGRAERHGLAQADYFNFETLAADRDSAEMQTTAAA
                     MGYARVLAEGRVRPETVEDLWEMQKNRVDLPVGLNDALAQNRLVDWFEGLAPTDIGYS
                     NLSAGYVRYRRLIREGGWPVFRQGATIEPGMSDARIPALIQRLVAEGDLSPADGARLT
                     QQGIVYGPELQAAVRGFQVRHGLSADGRIGTGSQRSLSASAEDRARQIALNLERRRWL
                     KREVNPERIEVNTAAASMV"
     gene            complement(1094..1318)
                     /gene="rpmE"
                     /locus_tag="VF686_05995"
     CDS             complement(1094..1318)
                     /gene="rpmE"
                     /locus_tag="VF686_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8661399.1"
                     /translation="MKADTHPDYHFITVTMTDGTTYQTRSTYQKEGAVLNLDIDPLTH
                     PAWTGGNQHMLDRGGRVSRFNNKFAGFIKK"
     gene            1452..>2175
                     /locus_tag="VF686_06000"
     CDS             1452..>2175
                     /locus_tag="VF686_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8661400.1"
                     /translation="MTDATATADAADIRGRPGAGAVLVEQLGEAGKRREKRRDIRPLG
                     RLLPYALRHKGHMAMAGVWLILSTAASLALTASARGAIDNGFDDGGSRLNLWFLLLGA
                     NALFLGLATAVRYYFVTKTGERVIADLRKGLFQRILTLDPAFYASMRTGEVLSRLTTD
                     IALVETLLTTSVSYALRNFLTLIGGTVLLFVVSPKLTGMVLLIVPLLLGPIFLFGRRV
                     RKLTVASQDRFANAVGFAGESVD"
BASE COUNT          334 a          696 c          791 g          354 t
ORIGIN      
        1 agaccatgct ggcggcggcg gtattgacct cgatccgctc gggattgacc tcgcgcttca
       61 gccagcggcg gcgttcgagg ttcagggcga tctggcgggc gcggtcctcg gccgaggcgg
      121 agagcgagcg ctggctgccc gtgccgatcc ggccgtcggc cgaaagacca tgccggacct
      181 ggaagccgcg cacggcggcc tgaagttcgg ggccgtagac gatgccctgc tgggtcaggc
      241 gagcgccgtc ggcgggcgac aggtcgccct cggcgacgag gcgctggatc agggcgggaa
      301 tgcgggcgtc gctcataccc ggctcgatcg tcgcgccctg acggaagacc ggccagccgc
      361 cctcgcggat gagacggcgg tagcggacat agccggcgga caggttggaa tagccgatgt
      421 cagtgggagc cagaccttcg aaccagtcga ccaggcggtt ctgggcgagg gcgtcgttca
      481 ggccgacagg caggtcgacg cgattcttct gcatctccca aaggtcttcg accgtctcgg
      541 gacgcacacg gccctcagcc agaacgcggg catagcccat ggcggcggcg gtggtctgca
      601 tctcggccga gtcgcgatcg gcggcgaggg tctcgaagtt gaaatagtcg gcctgggcca
      661 gaccgtggcg ttctgcgcgc ccggcggcgg cctgcagcgc gcgcaggcgt tcggcggtcc
      721 agaccggacg ccatccgttc agctcgtaga aggcccggac atccggctgc atgccagcgg
      781 ggagggtcgc cagctgatcg ttgaaccggt catagaaggc cacggcggcg ggatcgcggg
      841 tctgggccga agccgcctga cccaccgccg acgcaaacgc cgtcacggcc gcgcccaatc
      901 cgagttgccg tcgattcata gccaaaatct ctacgccggt tcgatgcggg acaccgtgtc
      961 ccgccccccg aaagggccaa cgccgccatg ttatcggcgt tccggccaaa cagaaaggcg
     1021 ccggtccggg gaccgacgcc tttgtggtcg acttgccgca gggtgtggcc cgaaagcccg
     1081 ccctgacagc ggcctacttc ttgatgaagc cggcgaactt gttgttgaag cgcgaaacgc
     1141 ggccgccgcg gtccagcatg tgctggttgc cgccggtcca ggccgggtgg gtcagcggat
     1201 cgatgtccag gttcaggacg gcgccttcct tctggtaggt cgagcgcgtc tggtaggtcg
     1261 taccatcggt catcgtcacg gtgatgaagt ggtagtcggg atgcgtatcc gccttcatgg
     1321 tcctggtccg gtctctgcgc gatgacgatg ccgaccgtcg gagcgcgaaa atgagaaacc
     1381 cgccgggatg acggcgggaa tgagcggcgg cgtttacacc gggggccgtt ccggggcaag
     1441 aggcgcgcgc catgacagat gcaaccgcca ccgcagacgc cgctgacatc cggggacgcc
     1501 ccggcgcggg ggccgttctg gtcgaacagc tgggcgaggc gggaaagcgg cgcgagaagc
     1561 gccgcgacat ccgcccgctg ggccggctgc tgccctatgc gctgcgtcac aagggccata
     1621 tggccatggc gggggtgtgg ctgatcctgt cgacagcggc ctcgctggcc ctgacggcgt
     1681 cggcgcgggg ggccatcgac aatggattcg acgacggcgg gtcgcggctg aacctgtggt
     1741 tcctgctgct gggcgccaac gccctgttcc tcgggctggc gaccgccgtc cgctattatt
     1801 tcgtgaccaa gacgggcgag cgcgtcatcg ccgacctgcg caagggactg ttccagcgca
     1861 tcctgacgct ggaccccgcc ttctacgcct ccatgcggac aggggaggtg ctgtcgcggc
     1921 tgacgaccga catcgccctg gtcgagacgc tgctgaccac ctcggtgtcc tacgccctgc
     1981 gcaatttcct gaccctgatc ggcgggacag tgctgctgtt cgtggtcagt ccgaagctga
     2041 cgggcatggt gttgctgatc gtgccgttgc tgctggggcc gatcttcctg ttcggccggc
     2101 gggtgcggaa gctgacggtg gcgtcgcagg accggttcgc gaacgcggtc ggcttcgccg
     2161 gcgagagcgt cgacg
//
LOCUS       DASVVN010000372         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_514394,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000372 DASVVN010000000
VERSION     DASVVN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514394"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VF686_06005"
     CDS             complement(<1..877)
                     /locus_tag="VF686_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8661401.1"
                     /translation="MIPVPGFEGRRIAVFGLGRSGLTAARALKAGGALSVLWDDSVSS
                     RMQAEAEGFVVEDLTAADWSGFAALVLSPGAPLTHPKPHWTVDKAKAAGVEVIGDIEL
                     FARAIAALPERERPKVVAITGTNGKSTTTALIGWVLKQAGLTVHVGGNIGIGVLALPE
                     PTPGAVYVIEVSSYQLDLTTSFAPGVGILTNVSPDHLDRHGGMEGYVAAKARIFRAQG
                     PDGLALVGVDESWGRAVAADLAAANRHLTTVSTSVHPREGGDPGLSGGGGVPVFQGRP
                     PRGAEAKAWVPAFAGM"
     gene            complement(882..>1712)
                     /gene="mraY"
                     /locus_tag="VF686_06010"
     CDS             complement(882..>1712)
                     /gene="mraY"
                     /locus_tag="VF686_06010"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX8661402.1"
                     /translation="WGDLTNPYIWIVSFVTAAFGVLGFIDDYAKVSKQTSAGLTSKQK
                     LVAQVIVSVIAGVMTVLWMTASPTTPNLETSIAFPFFKAVLLNVGWFYVVFAAFTLVG
                     FSNAVNLTDGLDGLAIVPVMMASAAFGIIAYLVGNFIFSDYLGVHHVPGAGELAVFCS
                     AIIGAGTGFLWYNAPPAKIFMGDTGSLALGGALGAIAVATKHELVLGIVGGLFVIEAA
                     SVMIQVAYFKATKKRIFLMAPIHHHFEKMGWPESTVVIRFWIVSAMLALAGLATLKLR
                     "
BASE COUNT          306 a          623 c          537 g          246 t
ORIGIN      
        1 tcatccccgc gaaggcgggg acccaggctt tagcctctgc acccctcggt gggcgcccct
       61 ggaacacggg gacgccgccg ccgccgctca atcctgggtc cccgccttcg cggggatgca
      121 cggatgtgga gacagtcgtg agatggcgat tggcggcggc caggtcagcg gcgacggccc
      181 gcccccagct ctcgtccacg cccaccagcg ccaacccatc cggcccctgc gcccggaaaa
      241 tccgcgcctt ggccgcgaca tacccctcca tcccgccatg ccggtccaga tggtccgggc
      301 tgacattggt caggatcccc acgcccggcg cgaagctcgt cgtcaggtcc agctgatagc
      361 tcgacacctc gatcacatag accgcgcccg gtgtcggctc cggcagggcc agcacgccga
      421 tgccgatgtt gccgccgacg tggaccgtca gccctgcctg tttcaacacc cagccgatca
      481 gggccgtggt cgtcgacttg ccgttggtgc cggtgatggc gaccaccttc ggccgctccc
      541 tctccggcag ggccgcgatg gcgcgggcga acagctcgat gtcgccgatc acctcgacgc
      601 ccgccgcctt cgccttgtcc acggtccagt gcggcttggg atgcgtcagc ggcgcccccg
      661 gcgacagcac cagcgccgcg aatcccgacc agtcggcggc ggtcagatcc tcgaccacga
      721 acccttcggc ctcggcctgc atccggctgg agacgctgtc atcccacagc accgacaggg
      781 ccccgccggc cttcagcgcg cgcgcggccg tcagccccga ccgccccagt ccgaagaccg
      841 cgatgcgccg cccttcaaag ccgggaacgg ggatcatgcg gctagcgcag cttcagggtg
      901 gcgagacccg ccagcgccag catggccgag acgatccaga accggatcac caccgtcgat
      961 tccggccagc ccatcttttc gaaatggtgg tggatcgggg ccatcaggaa gatgcgcttc
     1021 ttcgtggcct tgaaataagc gacctggatc atgaccgagg ccgcctcgat cacgaacaga
     1081 ccgccgacga tgcccagcac cagctcatgc ttggtcgcca cggcgatggc gcccagcgcg
     1141 ccgccgagcg ccagcgaacc cgtgtcgccc atgaagatct tggccggcgg agcattgtac
     1201 cagaggaagc cggtgcccgc cccgatgatg gccgagcaga agacggccag ttcgcccgcg
     1261 cccggcacgt ggtggacgcc cagatagtcc gagaagatga agttgccgac cagataggcg
     1321 atgatgccga acgccgccga cgccatcatc acgggcacga tggccagccc gtccagcccg
     1381 tccgtcaggt tcaccgcgtt cgagaagcca acgagagtga aggcggcgaa cacgacgtag
     1441 aaccagccca cgttcagcag cacggccttg aagaagggga aggcgatcga ggtctcgagg
     1501 ttcggcgtgg tcggcgaggc cgtcatccac agcacggtca tgaccccggc gatcaccgag
     1561 acgatcacct gggccaccag cttctgcttc gacgtcagcc cggccgaggt ctgtttcgac
     1621 accttggcgt agtcgtcgat gaagcccagc acgccgaacg ccgccgtcac gaagctgacg
     1681 atccagatat aggggttggt cagatcgccc ca
//
LOCUS       DASVVN010000373         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_461894,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000373 DASVVN010000000
VERSION     DASVVN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461894"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF686_06015"
     CDS             <1..591
                     /locus_tag="VF686_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8661403.1"
                     /translation="RICVQRASTSELNVADWFRSRGLEYTPVVTANEEAARAAYGRED
                     CDAFSADISALAAARTTLSNPEQHAILPDVISKEPLGPAVKRGDEAWTSIVRWTLNAL
                     ILAEELGVTQDNAGELAEDSRDPRVRRLLGTEGDFGAMMGLRRDWAKSAIEAEGNYGE
                     IFTRNVGTDSSLDLTRGLNAQWNATPGGLIYALPIR"
     gene            620..>1625
                     /locus_tag="VF686_06020"
     CDS             620..>1625
                     /locus_tag="VF686_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8661404.1"
                     /translation="METTKKGGVPLYLWMLLGFAVGLGGGLYVNLTGQTVIPEVMSII
                     QAVGQIFLRLLFMLVLPLLFAALAVGVAEMGDLKSLGRVGFKTLLFTVIVSAIAVGIG
                     LGMVNWFRPGDGVDPVLAQQLLAQGADGASAIVGNAPKSIEAGQFFLDMIPSNVFTAA
                     AENQILPVMIFALIFGIGMVMAKSPATDALQNALQGLLEVMMKLINLVIKLAPFAIAA
                     LMFNLSAVFGWDLLVRLGAYAGVAIGAMAIHMFIVYPLLVWIFGGMSPLKFANGVREP
                     FVVAFSTASSNATLPIAIKAAEEKLKLPRRISRFVLTVGATANQNGTALFEGVTVLFL
                     A"
BASE COUNT          262 a          516 c          557 g          290 t
ORIGIN      
        1 cggatctgcg tgcagcgggc ctcgacctcg gaactgaacg tcgccgactg gttccggtcc
       61 cgggggctgg aatacacgcc cgtggtcacc gccaacgagg aggcggcgcg cgcggcctac
      121 gggcgcgagg actgcgacgc cttcagcgcg gacatttcgg cgctggctgc ggcccgcacc
      181 accctgtcga atcccgaaca gcacgccatc ctgcccgacg tcatttccaa ggagccgctg
      241 gggccggcgg tcaaacgcgg ggacgaggcc tggacctcga tcgtgcgctg gacactgaac
      301 gcgctgatcc tcgccgagga actgggcgtg acgcaggaca acgccggcga gctggcggag
      361 gattcgcgcg atccccgggt gcgccgtctg ctcgggacag aaggcgactt cggcgccatg
      421 atgggactgc gcagagattg ggcgaagtca gcgatcgaag ccgagggaaa ctatggggaa
      481 attttcacgc ggaatgtcgg gacggattca tcgctcgacc tgacgcgcgg gcttaacgca
      541 caatggaacg cgacgccggg cggactgatc tacgccctgc ccatcagata acgacatcgg
      601 ccgggagccg gggggacgca tggaaacgac gaaaaagggc ggcgtgccgc tctatctgtg
      661 gatgctgctg ggctttgccg tcgggctggg aggcgggctt tacgtcaacc tgaccggcca
      721 gaccgtgatc cccgaggtga tgtcgatcat ccaagccgtg ggccagatct tcctgcgcct
      781 gctgttcatg ctggttctgc cgctgctgtt cgcggcgctg gcggtcggcg tggccgagat
      841 gggcgatctg aagtcgctgg gccgggtcgg cttcaagacc ctgctgttca ccgtcatcgt
      901 ctcggcgatc gccgtgggca tcggcctggg gatggtcaac tggttccgtc cgggcgacgg
      961 cgtcgatccg gtgctggccc agcagctgct ggcgcagggc gcggacggcg cgtccgccat
     1021 cgtcggcaat gcgcccaagt cgatcgaggc gggccagttc ttcctcgaca tgattccgtc
     1081 caacgtcttc acggcggcgg ccgagaacca gatcctgccg gtgatgatct tcgcccttat
     1141 cttcggcatc ggcatggtca tggccaagag cccggccacc gatgcgctcc agaacgccct
     1201 gcagggcctg ctggaagtga tgatgaagct gatcaatctg gtcatcaaac tggccccgtt
     1261 cgcgatcgcg gccctgatgt tcaacctgtc ggcggtgttc ggatgggatc tgctggtgcg
     1321 gctgggcgcc tatgccggcg tcgccatcgg cgcgatggcg atccacatgt tcatcgtcta
     1381 cccgctgctg gtctggatct ttggcggcat gagcccgctg aagttcgcca acggcgttcg
     1441 ggagccgttc gtggtggcct tctcgacggc atcgtccaac gccaccctgc cgatcgccat
     1501 caaggccgcg gaagagaagc tgaagctgcc gcgccgcatc tcgcgcttcg tactgacggt
     1561 gggcgccacg gccaaccaga acggcaccgc cctgttcgag ggtgtgacgg tcctgttcct
     1621 ggccc
//
LOCUS       DASVVN010000374         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_279245,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000374 DASVVN010000000
VERSION     DASVVN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279245"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..346)
                     /locus_tag="VF686_06025"
     CDS             complement(122..346)
                     /locus_tag="VF686_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661405.1"
                     /translation="MSSIRPDLPSALFPTHPAGQSPVRASRSDFFKAALEKAAGPAAE
                     PSLRAAAPQPTPAAFEERPTRPGALLDIRV"
     gene            complement(391..960)
                     /locus_tag="VF686_06030"
     CDS             complement(391..960)
                     /locus_tag="VF686_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX8661406.1"
                     /translation="MNRWFSVIPLVVLAALAALFIGWSLKRDPAVKPDALVGQSVPET
                     VLPILNGVTPGPGHVDLKTAGVGKPMLINVFASWCLPCRAEHPKLMALKARGVAVVGV
                     AWKDDPADTRRFLDELGDPYSMVLVDREGRAGLDLGITGAPETFAVNAMGQVVAKFSG
                     PMVDDAEIERMIAAIQAPPRPLPTATSRD"
     gene            complement(957..1154)
                     /gene="ccmD"
                     /locus_tag="VF686_06035"
     CDS             complement(957..1154)
                     /gene="ccmD"
                     /locus_tag="VF686_06035"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX8661407.1"
                     /translation="MIDLDMGAYAAFVWPAWGISAVVLAALAMRAIVAARRWNAALRQ
                     VDNDDSRSRPQVARSAVGQPE"
     gene            complement(1157..1876)
                     /gene="ccmC"
                     /locus_tag="VF686_06040"
     CDS             complement(1157..1876)
                     /gene="ccmC"
                     /locus_tag="VF686_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX8661408.1"
                     /translation="MFLANPDRFLRFTRPLTPVLWALAGVLLMVGTWLSFAAPEDYQQ
                     GDTARIMFVHVPAASVGLMIYAALGVSSFFALVFRHPLADAAARAAALPGAAYTLLAL
                     VTGSLWGKPMWGAWWAWGDARLMSVLVLFLFYLGYMALRASIDDEAKAARAAAVLGLV
                     GLVNLPIVKFSVDWWNTLHQPASLIRAEGPSMPAVFLAPLLTMMAAYGVFFLAVWITS
                     IRTEIVRRRVLSLRARRAREA"
     gene            2000..2224
                     /locus_tag="VF686_06045"
     CDS             2000..2224
                     /locus_tag="VF686_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEX8661409.1"
                     /translation="MTDDVTKDSNGNVLADGDSVTLIKDLKVKGSGGVTLKRGTLVKN
                     IRLTGDTDEIEANVEKVRGLVLRTEFVKKA"
     gene            complement(2279..>2722)
                     /locus_tag="VF686_06050"
     CDS             complement(2279..>2722)
                     /locus_tag="VF686_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8661410.1"
                     /translation="LEDGALDLIALGPIPLELIVLTKAKAQWLAVGAPLALTAPVAAI
                     TLGLPPALAPLVALSALIGALGFALTGALGAALALGAKRGGLLIAVVVLPLFIPPVVF
                     GAGALERAANGLSPLPALAFLFAYVLFASVVTPLAAAAAIRNAQG"
BASE COUNT          485 a          864 c         1029 g          344 t
ORIGIN      
        1 agcaatgcgg ccgcgaaagc cgccgcagga atgatccaac ccctcgccat gggcgggagg
       61 ttaatcatgg cgagcgaaaa cgcgccgctc attttcgcgt gaaggccgag cgagcagacc
      121 gctagacgcg gatgtccagc agggcgcccg ggcgggtcgg gcgctcctcg aaggcggccg
      181 gggtcggttg cggcgcggcg gcgcgaaggc tcggctcggc ggcgggacct gcggcttttt
      241 ccagcgcggc cttgaagaag tccgagcgcg aggcgcgcac gggagattgg ccggcggggt
      301 gcgtcgggaa cagggccgag ggcaggtcgg gacgaatcga actcatgccg acagggttaa
      361 ccaaacccgt cgaaaatggg ttaacgcgcc ctagtcgcgg gatgttgcgg tgggcagggg
      421 gcgaggcggc gcctggatgg cggcgatcat ccgctcgatt tcggcgtcgt cgaccatggg
      481 gccgctgaac ttggccacga cctggcccat ggcgttgacg gcgaaggtct cgggcgcgcc
      541 ggtgatgccc agatccagcc cggcccggcc ctcccggtcg accaggacca tggaataggg
      601 gtcgcccagc tcgtcgagga aacggcgggt gtcggcgggg tcatccttcc aggccacccc
      661 gaccacggcg acgccgcgcg ccttcagggc catcagtttc ggatgctcgg cgcggcaggg
      721 caggcaccag ctggcgaaga cattaatcag catcggcttg ccgacgccgg cggtcttgag
      781 atcgacatgg ccggggccgg gggtgacgcc gttcaggatc ggcagcacgg tctcggggac
      841 ggactggccg acgagggcgt cgggcttcac cgcgggatcg cgcttcagcg accagccgat
      901 gaacagggcg gcgagggcgg ccagaacgac cagcgggata acggagaacc agcggttcat
      961 tctggttgcc ctaccgcgct tcgcgctact tgagggcggg atcgggagtc gtcattgtcg
     1021 acctgcctga gcgccgcgtt ccagcgccgc gcggcgacaa tggcgcgcat cgccagtgcg
     1081 gcgaggacga cggcgctgat cccccaggcg ggccagacga aggcggcgta ggcgcccatg
     1141 tcgaggtcga tcatcttcag gcctcccggg ctctgcgggc gcgcaggctc agcacccggc
     1201 ggcggacgat ctcggtgcgg atcgaggtga tccagacggc gaggaagaag acgccatagg
     1261 ccgccatcat ggtcagcagc ggcgcgagga agacggcggg catcgacggt ccctcggcgc
     1321 ggatcagtga agcgggctgg tgcagggtgt tccaccagtc gaccgagaat ttgacgatcg
     1381 gcagattgac cagaccgacc agccccagca cggcggcggc gcgggccgcc ttggcctcgt
     1441 cgtcgatcga ggcgcgcaag gccatgtagc cgaggtagaa caggaacagc accagcaccg
     1501 acatcagccg cgcatcgccc cacgcccacc aagcgcccca catgggcttg ccccacaggc
     1561 tgccggtgac cagcgccagc agggtgtagg ccgcgccggg cagggcggcg gcgcgcgcgg
     1621 cggcgtcggc cagcggatgg cggaagacga gggcgaagaa gctggagacg cccagcgccg
     1681 catagatcat cagcccgacc gaggcggccg ggacgtgcac gaacatgatc cgcgccgtgt
     1741 cgccctgctg atagtcctcg ggcgcggcga aggagagcca ggtcccgacc atcagcagca
     1801 ccccggccaa cgcccacagc accggcgtca gcggccgggt gaagcgcagg aagcggtcgg
     1861 ggttggcgag gaacatcggc gctctttagt gcgctaccga gcttcgcgct acttgagcgc
     1921 ggcccggccg tggcggtttg cgctatttgg acgcggatgc tgtgctagag cccccgctcg
     1981 caaccctgaa agccccgcca tgaccgacga cgtgaccaag gattccaatg gcaatgttct
     2041 ggccgacggc gacagcgtga ccctgatcaa ggacctgaag gtcaagggct cgggcggggt
     2101 gacgctgaag cgcggcacgc tggtgaagaa catccgcctg accggcgaca ccgacgagat
     2161 cgaggccaat gtggaaaagg tgcgcgggtt ggtgctgcgg acggagttcg tcaagaaggc
     2221 ctgaggttct tgaatccggg catgcctgcc ttcgcggaga tgcacggatg atttgggttc
     2281 agccctgcgc attccggatc gccgccgccg ccgcgagggg cgtgaccacg gacgcgaaca
     2341 ggacgtaggc gaacaggaag gcgagcgcgg gcaaggggct gagcccgttc gccgcccgct
     2401 ccagagcgcc cgcgccgaac accacgggcg ggatgaacag cggcaggacc acaaccgcga
     2461 tcagcaggcc gccgcgtttg gccccgaggg cgagggcggc gcccagcgct ccggtcaggg
     2521 cgaagccgag ggcgccgatg agggcggaca gggccaccag cggcgccagc gcggggggca
     2581 ggccgagggt gatcgccgcg accggcgcgg tgagggccag aggggcgcca acggcgagcc
     2641 actgggcctt ggccttggtc aggacgatca gctccagcgg gatcgggccg agcgcgatca
     2701 gatcgagggc tccgtcctcg ag
//
LOCUS       DASVVN010000375         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_487928,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000375 DASVVN010000000
VERSION     DASVVN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487928"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VF686_06055"
     CDS             complement(<1..790)
                     /locus_tag="VF686_06055"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX8661411.1"
                     /translation="MNLVVVESPAKAKTINKYLGPDFTVLASYGHVRDLPSKDGSVLP
                     DDDFAMSWEIDAKASKRLSEIAEVAKKSDRVILATDPDREGEAISWHVLEILTKKKVL
                     KDTHVERVTFNAITKSAVLEAMANPRQIDMELVEAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRIVVDREMEIEKFRAQEYWSVEADLNADSPPFTTRLVKHAGKRV
                     QRLDITSEAMATAARDAINAGTFTIASVEKKPVKRAPAPPFMTST"
     gene            complement(881..1975)
                     /gene="dprA"
                     /locus_tag="VF686_06060"
     CDS             complement(881..1975)
                     /gene="dprA"
                     /locus_tag="VF686_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX8661412.1"
                     /translation="MSPLPPLSEAERFARLRLARTDRVGPVTFRQLLDRYETAERALE
                     ALPELVRRAGGRGEGPPPIGQIENELAVGERIGARLIVLGDAAYSEPLAATDPPPPVL
                     WTLGDAALMSRPCIAVVGARIASAGGQRIARGLAQQLGEAGHVVVSGLARGIDGAAHA
                     GALPTGTVAVLGGGVDDVYPPDNADLYRQIVERGCIVSESPVGARAQAKDFPRRNRII
                     SGLSRGVIVVEAELRSGSLITARLAGEQGRDVFAVPGSPLDPRSKGPNELLRQGAILC
                     EGLEDVERAFSTLRTLREPGPDNPFRDAPGEVDGALIERVAALLSPTPTPRDELARAT
                     GAPVGAVAAALLELSLAGRATLLPGGLASI"
     gene            complement(1972..2625)
                     /gene="plsY"
                     /locus_tag="VF686_06065"
     CDS             complement(1972..2625)
                     /gene="plsY"
                     /locus_tag="VF686_06065"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX8661413.1"
                     /translation="MQDLVGPALGTLALVAVGGYLLGSIPFGVILTRLMTGEDVRSIG
                     SGNIGATNVLRTGRKDLAFATLILDAGKGAAALLLARYMLGGVSHSAEVAGAIAGGAA
                     FMGHLFPVWLKFKGGKGVATFFGLLLAACWPLGLLAGATWLLMAAVFRMSSLAALIAA
                     VAAPIYAILPLAALGLPAPTPVAALAVFTGVLIWIRHHENIARLLKGTEPRIGASKG"
BASE COUNT          424 a          992 c          928 g          393 t
ORIGIN      
        1 gggtcgaggt catgaaaggc ggggccgggg cgcgcttgac cggcttcttt tccaccgagg
       61 cgatggtgaa ggttcccgcg ttgatggcgt cccgcgccgc ggtcgccatg gcttccgagg
      121 tgatgtccag ccgctgcacc cgcttgccgg cgtgcttgac cagacgcgtg gtgaagggcg
      181 ggctgtcggc gttcaggtcg gcctcgacgg accagtattc ctgcgccctg aacttctcga
      241 tctccatctc gcgatcgacg acgatgcgca gggcgaccga ctgcacccgc ccggccgagc
      301 gcgcgcccgg cagcttgcgc cacagcaccg gcgacagggt gaagcccacc agatagtcca
      361 gcgcgcggcg ggccagatag gcctcgacca gctccatgtc gatctggcgc ggattggcca
      421 tcgcctcgag cacggcgctc ttcgtaatgg cgttgaaggt gacccgctcg acatgcgtgt
      481 ccttcagcac cttcttcttc gtcagaatct cgagcacgtg ccagctgatc gcctctccct
      541 cgcggtcggg gtcggtggcc aggatgacgc ggtcggattt cttggcgacc tcggcgatct
      601 cgctcagccg cttggaggcc ttggcgtcga tctcccacga catggcgaaa tcgtcgtcag
      661 gcagcacgga cccgtccttg gacggcagat cgcggacatg cccgtaggac gccaggacgg
      721 tgaagtccgg gcccagatat ttgttgatgg tcttggcctt tgcggggctc tcgacgacga
      781 cgaggttcat gcgggaaggg ggccttgaaa cggcgcggaa agggggcgga acgtggttgg
      841 agcgagcgtc gctgtcaatc ggcgctgaaa ttcggatcgt ctagatcgag gcaaggccgc
      901 ccggcagaag ggtcgcgcgg cccgccaggc tgagctccag cagcgccgcc gccaccgctc
      961 ccaccggcgc gcccgtcgcc cgggccagct catcccgtgg cgtcggcgtc ggcgacagaa
     1021 gggccgcgac ccgctcgatc agtgcgccat ccacctcgcc aggcgcgtcc cggaacggat
     1081 tgtctggtcc gggctcgcgc agcgtgcgca gcgtcgagaa ggcgcgctcc acgtcctcca
     1141 gcccctcgca caggatcgcc ccttgccgca gcagttcgtt gggccccttc gagcgaggat
     1201 cgagcggcga gcccggcacg gcgaagacgt cgcgtccctg ttccccggcc agccgggccg
     1261 tgatcagcga tccggacctc aactcggcct cgaccacgat cacgccgcgc gacaggcccg
     1321 agatgatgcg gttgcggcgc ggaaagtcct tcgcctgcgc ccgcgccccc accgggcttt
     1381 cggagacgat gcagccccgt tcgacgatct ggcgatagag gtcggcgttg tccggcggat
     1441 agacgtcgtc cacgccgccg cccagcaccg ccaccgttcc ggtcggcaga gcccccgcat
     1501 gcgccgcccc gtcgatgccg cgcgccagtc ccgacacgac cacatggccc gcctcgccca
     1561 gctgctgcgc cagccctcgc gcgatccgct ggcccccggc ggaggcgatc cgggccccca
     1621 ccacggcgat gcagggccgg ctcatcagcg cggcgtcgcc cagagtccac aggacgggcg
     1681 gcggcgggtc cgtcgccgcc agcggttcgg aataggcggc gtcgcccagc acgatcagcc
     1741 gggccccgat ccgctcgccc acggcgagtt cattctcgat ctgtccgatc ggcggcgggc
     1801 cctcgccgcg gcctccggcg cgccgcacca gctccggcaa agcctccagc gcgcgttcgg
     1861 ccgtctcgta ccggtccaga agctgccgga aggtcacggg accgacccgg tccgtgcgcg
     1921 ccagcctcag ccgcgcgaat cgctccgcct ccgacagggg cgggagcggg ctcacccctt
     1981 gctggcgccg atgcggggtt ccgtcccctt cagcaggcgg gcgatattct cgtggtggcg
     2041 gatccagatc aggacgccgg tgaacaccgc cagggctgcg accggcgtcg gcgcgggcag
     2101 acccagggcc gcgaggggca gaatggcgta gatcggcgcg gcgacggcgg cgatcagcgc
     2161 cgccagtgaa gacatccgga acacggccgc catcagcagc caggtcgcgc cggccagcag
     2221 gcctaagggc cagcaggcgg ccagcagcag gccgaagaag gtcgccaccc ccttgccgcc
     2281 cttgaacttg agccagacgg ggaacaggtg acccatgaag gccgcgccgc cggcgatggc
     2341 gcccgcgacc tcggcggaat ggctcacgcc gcccaacatg tatctggcca gcaacagcgc
     2401 cgccgcgccc ttgccggcgt ccaggatcag ggtggcgaag gccagatcct tgcggcccgt
     2461 ccgcaggacg ttggtcgcgc cgatattgcc cgaaccgatg ctgcgcacgt cctcgccggt
     2521 catcaggcgc gtcaagatca ccccgaacgg aatggaaccc agcaggtagc cgccgaccgc
     2581 cacaagggcg agcgtcccga gagcgggtcc gaccagatcc tgcacttcaa ccccctacag
     2641 ccggagcgtg tatatcacgg cttcacccgt cgtgaagcgc cgcagtttcc gatgattcgc
     2701 ggcgcgccgg tcagcgaccc tcgtaaaccc ggcggcc
//
LOCUS       DASVVN010000376         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_409190,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000376 DASVVN010000000
VERSION     DASVVN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409190"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..739)
                     /locus_tag="VF686_06070"
     CDS             complement(119..739)
                     /locus_tag="VF686_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8661414.1"
                     /translation="MKLYTSHRAPNPRRVRWVMAEKGIEDVELVEIDIMVGEHKTAEY
                     RSRVGVPHVPALELDDGTTISESIAIGRYLEALYPEPNLFGADAREQAVIEMWTRRCE
                     FYLANPIMLSVRHSHPALAALEAAQMPQLADYNRVAAEKFMKTLDRRLAENEYLAEGR
                     FSIADIVAVVGLDFARLIKYRPPEELTHLARWLETCRARPGAKAGA"
     gene            complement(770..2041)
                     /locus_tag="VF686_06075"
     CDS             complement(770..2041)
                     /locus_tag="VF686_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661415.1"
                     /translation="MFSLLNLQSLLGLVVIVGACWALSENRRAFPWRLTLGAIAVQAA
                     LVLALFAIPGSQNVLAAITGAVDGLATATNRGTQFVFGYLAGGDQPYAVQNDGALFTF
                     AFQVLPLILVISALSALLWHWKILKWITHGFGILFQRTLGLGGASALAVAANIFLGMI
                     ESPIVIRAYLDKLTRSELFLMMVVGLATVAGSTMVAYAAILSPVMTNAAGHVLVASIV
                     SAPAGVLLARIMIPETAGEGGAAADYDSALKYDSAIDAIVKGTADGLMVVLNISAVLI
                     VFVALVALVNVMLGGFWLFGDHVTVERVLGWLFMPVAWLTGVEWSEAGKAGWLLGVKL
                     TLTEFVAFIELGKVPAGEMSERTRMLMTYALCGFANIGSVGITVTGLSVLMPERREEV
                     LGLVWKGLFAGFLATLMTAAIVGAMPAAIFR"
     gene            complement(2220..>2471)
                     /locus_tag="VF686_06080"
     CDS             complement(2220..>2471)
                     /locus_tag="VF686_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661416.1"
                     /translation="AGRTCNFGGGGVSTRCSAAGATALGRGVGSMMLTSRTSRAAVSF
                     EAFSCLASWQADRTIALAAASIRAFRIIILPVAVVFTVT"
BASE COUNT          449 a          887 c          771 g          364 t
ORIGIN      
        1 catgctttcc tgcctccgac gggtctttga caactgaatc agcagcgaag agcctccgcc
       61 cgcgtgcgcc gagtagacgc tgtagacggt gtagacgctg tagactgtgt ttttcggcct
      121 aggccccggc cttcgccccc ggccgcgccc ggcaggtctc cagccaccgc gccagatgcg
      181 tcagttcctc cggcggccgg tatttgatca gccgcgcgaa atccaggccg accacggcga
      241 cgatgtcggc gatgctgaac cggccctcgg cgaggtactc gttttcagcc agacgccggt
      301 ccagggtctt catgaatttc tcggccgcca cccggttgta gtccgccagc tggggcatct
      361 gcgccgcctc cagtgcggcc agcgccgggt gcgagtgccg cacgctcagc atgatcggat
      421 tggccagata gaattcgcag cggcgggtcc acatctcgat caccgcctgc tcgcgggcgt
      481 cggcgccgaa caggttcggc tcgggataca gcgcctccag ataccggccg atggcgatgg
      541 attccgagat cgtcgtgccg tcgtccagct ccagcgccgg cacatgcggc acgcccacgc
      601 ggctccggta ttcggcggtc ttgtgctcgc caaccatgat gtcgatctcg accagttcga
      661 cgtcctcgat gcccttctcg gccatgaccc agcgcacgcg gcggggatta ggggcgcggt
      721 gcgaggtgta gagcttcatc ggggaagtcc ttggggaggg agcttggcgt cagcggaata
      781 tggccgccgg catcgcgccc acaatggccg cggtcatcag cgtggccaga aaaccggcga
      841 acagcccctt ccacaccaga cccagcacct cttcccgccg ctcgggcatc agcacgctga
      901 gccccgtcac cgtgatgccg accgagccga tgttggcgaa gccgcacagg gcgtaggtca
      961 tcagcatccg cgtccgctcg ctcatctcgc cggccgggac cttgcccagt tcgatgaagg
     1021 cgacgaactc ggtcagggtc agcttgaccc ccagcagcca gccggccttg cccgcctcgg
     1081 accactcgac cccggtcagc caggccaccg gcatgaacag ccaccccagc acccgctcga
     1141 cggtgacatg gtcgccgaac agccagaacc cgcccagcat gacattgacc agcgcgacca
     1201 gcgccacgaa cacgatcagc accgccgaga tgttcagcac caccatcagg ccgtcggccg
     1261 tgcctttgac gatggcgtcg atggcgctgt cgtacttcag cgcggaatcg tagtcggccg
     1321 ccgccccgcc ctcgcccgcc gtctccggga tcatgatccg ggccagcagc acccctgccg
     1381 gcgccgaaac gatcgaggcg accagcacat ggcccgccgc attggtcatc accggcgaca
     1441 ggatggccgc ataggccacc atggtcgacc cggccacggt ggccaagccg accaccatca
     1501 tcaggaacag ctcggaccgg gtcagcttgt ccagataggc gcggatcacg atcgggctct
     1561 cgatcatgcc gagaaagatg ttggccgcca ccgccagcgc cgaggcgccg cccagcccca
     1621 gggtccgctg gaacaggatg ccgaaaccat gggtgatcca cttcaggatc ttccagtgcc
     1681 acagcagggc cgacagggcc gagatcacga ggatcagcgg caggacctga aaggcgaagg
     1741 tgaacagggc gccgtcgttc tgcaccgcat agggctgatc gcccccggcc agataaccga
     1801 acacgaactg ggtgccccga ttggtcgccg tcgccagtcc gtccaccgcc cccgtgatcg
     1861 cggccagcac gttctgcgag cccgggatgg cgaacagggc cagcaccagc gccgcctgca
     1921 ccgctatggc gcccagcgtc agccgccacg gaaacgcccg ccggttctcg gacagggccc
     1981 agcaggcgcc gacgatgacg acgagcccca gcaggctctg gagattcagc aggctgaaca
     2041 tgacggcgta ccccaccggc tccccgccgg tcccgtcccg ttgaacgcca gacggcgctg
     2101 tctggcaagc cgccgattgc ccgtgatcaa cgccgggaaa ccatgccgtg atttcgacgc
     2161 ctcacggcca aggttcggct gcgcgcgagc ccacacttcg cccgcatccg ggcgcgccat
     2221 cacgtcaccg tgaacaccac ggcgacaggg agaatgatga tgcgaaaggc tctgatgctg
     2281 gctgccgcca gcgcaatggt tctgtccgcc tgccaagacg cgaggcagga aaatgcttca
     2341 aacgacaccg cggcgcggga ggtgcgagag gtcaacatca tcgagcccac gcctcttccc
     2401 agagcggtcg ctccggctgc ggagcagcgc gtggaaacgc cgccgccgcc gaagttgcag
     2461 gtgcggcccg c
//
LOCUS       DASVVN010000377         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_199349,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000377 DASVVN010000000
VERSION     DASVVN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199349"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VF686_06085"
     CDS             <1..959
                     /locus_tag="VF686_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8661417.1"
                     /translation="TLHLLEDTGVTARILAEAQVMRTLFLHHNAKPLTTLRPDWKALG
                     ADHPMVILPQARTEALLTEALAPLGVVPERGKALAAVSQTDDLVTATLADGEVIQAPL
                     LFAADGAHSTVRKALDLDFPGDGWEEPWQLADVDIDGLQPDQGWIDLHDEGALIVLPF
                     SGKTFRLIGFGRPLMEGLPSQWTLGTVHWRSDFRISHRMVERMTVGRFCLAGDAAHIH
                     SPLGARGMNLGIEDAFVYAACAADFLNGEPGRLEDYNRIRHPVDAAVVDRVRGLTGMV
                     RNTSPLADWLKGVVPPLLSHLPFALNAGLRVGMGLDHPVTVR"
     gene            970..>1535
                     /locus_tag="VF686_06090"
     CDS             970..>1535
                     /locus_tag="VF686_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8661418.1"
                     /translation="MTSPLEATPDPLLTLEPHRRATVREIFGVDSDMTVPVFTERDSH
                     VPEIDEAYRFDPQTTLAICAGFAFDRRVMVQGYHGTGKSTHIEQVAARLNWPMVRVNL
                     DSHVSRIDLVGKDAIVLKDGKQITEFREGMLPWAIQRPIALVFDEYDAGRPDVMFVIQ
                     RVLETEGKLTLLDQNRVIRPHPYFRLFAT"
BASE COUNT          232 a          592 c          466 g          245 t
ORIGIN      
        1 gcaccctgca cctgttggag gacaccggcg tcaccgcccg tatcctcgcc gaggcgcagg
       61 tgatgcggac cctgtttctg caccacaacg ccaagccgct cacgaccctc aggcccgact
      121 ggaaggcgct gggcgccgac catcccatgg tcatcctgcc ccaggcccgc accgaggccc
      181 tgctgaccga ggccctggcc ccgctcggcg tggtcccgga gcgcggcaag gcgctggccg
      241 ccgtgtcgca gaccgacgat ctcgtcaccg ccaccctcgc cgacggcgag gtgatccagg
      301 cgcccctgtt gttcgccgcc gacggcgccc actccacggt gcgcaaggcg ctggatctcg
      361 acttccccgg cgacggctgg gaggagccct ggcagctggc cgacgtcgac atcgacggcc
      421 tgcagcccga tcagggctgg atcgacctgc atgacgaggg cgccctgatc gtcctgccct
      481 tctcgggcaa gaccttccgc ctgatcggct tcggccgtcc gttgatggag ggcctgccgt
      541 cccaatggac cctcgggacc gtccactggc ggagcgactt ccgcatttcc catcgcatgg
      601 tcgagcggat gaccgtcggc cgcttctgcc tcgccgggga cgccgcccac atccattcgc
      661 cgttgggcgc gcgcggcatg aacctcggca tcgaggacgc cttcgtctac gccgcctgcg
      721 ccgccgattt cctgaacggc gagcccggcc ggctcgagga ctacaaccgc atccgccacc
      781 cggtggacgc cgccgtcgtc gaccgcgtgc gcggcctgac cggcatggtc cgcaacacct
      841 cgccgctggc cgactggctg aagggcgtgg tcccgcctct gctctcgcat ctgcccttcg
      901 cgctcaatgc gggccttcgt gtcggcatgg gcctcgacca cccggtgacg gtgcggtaat
      961 cgcaccacca tgacctcccc gctcgaagcc acccccgatc ccctgctgac gctggaaccg
     1021 catcgccgcg ccaccgtgcg cgagatcttc ggcgtcgact ccgacatgac cgtgccggtc
     1081 ttcaccgagc gcgacagcca tgtgcccgag atcgacgagg cctaccgttt cgacccgcag
     1141 acgaccctgg ccatctgcgc cggcttcgcc ttcgaccgcc gcgtcatggt ccagggctat
     1201 cacggcaccg gaaagtcgac ccatatcgaa caggtcgccg cccgcctgaa ctggccgatg
     1261 gtgcgcgtga acctcgacag ccacgtcagc cgcatcgatc tggtcggcaa ggacgccatc
     1321 gtcctcaagg acgggaaaca gatcaccgag ttccgcgagg gcatgctgcc ctgggccatc
     1381 cagcgcccga tcgccctggt cttcgacgaa tacgacgccg gccgtcccga tgtgatgttc
     1441 gtcatccagc gcgtcctcga gaccgagggc aaactgaccc tgctcgacca gaaccgggtg
     1501 attcggccgc atccctattt ccgcctgttc gcgac
//
LOCUS       DASVVN010000378         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_199360,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000378 DASVVN010000000
VERSION     DASVVN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199360"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..>1803
                     /locus_tag="VF686_06095"
     CDS             192..>1803
                     /locus_tag="VF686_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX8661419.1"
                     /translation="MSKRTVHRTAPALRRRPVLMATAAVVALMGLPYGSVRAQEPGVA
                     SQEGGASAVAGGLGRPSGADVVRAPHTSEGTARLPRGETVVREPHTPALPRIGRNALP
                     RDPNVVAGGATFAEDGSRLDINQSTDRVVIDWRSFDIGQDAEVNFYQPGTSSIAVNRI
                     SQGGVPSQIQGILRANGTVVILNPNGVIFSATSRVDVGGLVASTGRMDVNQFMAGSNR
                     LDFTDATSGEIVMGGSINIAAAGLAAFVAPHVRNDGVINARLGRVALAAGETFTLDLA
                     GDRLIELGLGSNSPLVENFGSILVDGGRVQLSAVAAGAVVDQVINAGGLISVASARAE
                     GGTIVLEGANGGVTVSGELNASGAAGGGDIALSGATIETTAGAVLRADATDNGDGGSI
                     VSMAAGRGDYAGAFSARGGASGGDGGFVETSGKHVRIDGGISIDTRAPVGETGSWTID
                     PDDLTVVAGVAGTIGSEDSEIGADTIEAQLATTGVTLAARNSITVNADIDTTAGPASS
                     STLALTDQDGGGLTINLNAAIRLRAGQTL"
BASE COUNT          271 a          671 c          603 g          258 t
ORIGIN      
        1 gcgtcggcgt gcgcatcgcc gccaacgaca actgggccgc gaccctggaa tacgcccaac
       61 ccttcgaacg ggacatcgca tcccgcggcg accgggatgg ccgcctcttc ttctcgatct
      121 cagccgcctt ttaaggctcg acacaaaatc cgcgccccgc cccgggcgaa aacggggctc
      181 tggagacgac catgagcaag cgcaccgtcc accgcaccgc accggccctc cgccgccgtc
      241 ccgtcctgat ggccacggcg gcggtcgtcg ccctgatggg cctgccgtac ggatccgtcc
      301 gggcccagga accgggcgtg gcctctcagg aaggcggcgc gtcggccgtt gcgggcggat
      361 tgggccgtcc cagcggcgcg gatgtcgtgc gcgcgcctca cacttcggaa ggaacggcgc
      421 ggctgccacg cggcgagacc gtcgtgcgcg agccccatac cccggccctc ccccggatcg
      481 ggcgcaacgc cctgccccgg gaccccaatg tggtcgccgg cggagccacc ttcgccgagg
      541 acggttcacg cctcgatatc aatcagtcca cggaccgtgt ggtcatcgac tggcgcagct
      601 tcgacatcgg ccaggacgcc gaggtcaact tctaccaacc gggcacgagc tccatcgccg
      661 tcaaccgcat ctcgcagggc ggtgtgccca gtcagatcca gggcattctg cgcgccaacg
      721 gcacggtggt gatcctcaac cccaacggcg tcatcttctc ggcgacctcg cgtgtcgacg
      781 tgggcggtct tgtcgcctcg accggccgca tggacgtcaa ccagttcatg gccggttcga
      841 accgcctcga tttcaccgac gcgacctcgg gcgaaatcgt gatggggggc tcgatcaata
      901 tcgccgccgc gggcctggct gccttcgtcg cgccgcatgt gcgcaacgac ggcgtcatca
      961 acgcccgtct cggccgtgta gctctggccg cgggcgaaac cttcacgctc gacctggccg
     1021 gcgatcggct gatcgagctg gggctcggct ccaactcgcc gctggtcgag aatttcggct
     1081 ccatcctggt ggacggcggg cgggtgcagc tcagcgccgt ggccgcgggc gccgtggtcg
     1141 atcaggtcat caacgccgga ggtctcatct cggtcgcttc ggcccgtgcc gagggcggaa
     1201 ccatcgtgct ggagggcgcc aacggcggcg tgaccgtcag cggcgagctg aacgcctcgg
     1261 gcgcggccgg cggcggcgac atcgccctct ccggcgcgac catcgaaacc acggcgggcg
     1321 cggtcctgcg cgccgacgcc acggataacg gggacggcgg ctccatcgtc tcgatggcgg
     1381 ccggccgcgg cgactacgcc ggcgccttct cggcccgcgg cggcgccagc ggcggcgacg
     1441 gcggcttcgt ggaaacctcg ggcaagcatg tccggatcga cggcggcatc agcatcgaca
     1501 cccgggctcc ggtcggtgag accggctcct ggaccatcga ccccgatgac ctgaccgtgg
     1561 tggccggcgt ggcgggcacg atcggttccg aggattccga gatcggtgcc gacacgatcg
     1621 aggcccagct ggccacgacc ggcgtgacgc tcgccgctcg caactcgatc accgtcaacg
     1681 cggacatcga caccaccgcg ggtccggcct ccagctcgac cctcgccctg accgaccagg
     1741 acggcggcgg cctgacgatc aacctgaacg cggccatccg cctgcgcgcc ggccagacgc
     1801 tca
//
LOCUS       DASVVN010000379         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_199391,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000379 DASVVN010000000
VERSION     DASVVN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199391"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF686_06100"
     CDS             <1..327
                     /locus_tag="VF686_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX8661420.1"
                     /translation="TFHKHVHQLCNGVQIHVDDPAYDHAAFRPWRLQALGFKAIRRLY
                     PDYALWRDFAYEYVFDKLAIDVINGGSGLREWVDDPASTPADLEALTGPDEAAWAELR
                     KPYLLY"
     gene            complement(330..1151)
                     /locus_tag="VF686_06105"
     CDS             complement(330..1151)
                     /locus_tag="VF686_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8661421.1"
                     /translation="MMWSLAASAAVLALAPAAPAAAQTADAPLRPFCPDRPGKGDSTC
                     VMDQGHVQVEVGLFDTARQDDDATRAESWAAGTVVVRYGVGPSTEVQAGFSTWQRDKV
                     TDRSTGLTQRAEGLGDLFLAVRHGFVRPDGGGTGLAVTAFATLPTGGEEVRVSGVEGG
                     LALPVAVDLGGDWGLGVSPIVEYVRDGDGEGSHGAWSLVVGLGRPVGDWSLGAEVWVS
                     RDDDPLGASTQATFDLLAAWSPPFMTDAQLDFGLNLGLNDDSPDLEFGIGIARRF"
     gene            1274..>1756
                     /locus_tag="VF686_06110"
     CDS             1274..>1756
                     /locus_tag="VF686_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661422.1"
                     /translation="MIALLFAALLTFQEPAPLSADARALIAPVAEAIAAEEARQAALP
                     PPANDREKLERMGVLDQVGRRALTPIDLTVLPEAERAAANTAMWAPLTAMDERLMAEL
                     LTMVPPEGWFLKSVYGDQAASAAFLIVQHSDLENWRRFVPVLEPLVAAGEVNGQSYGL
                     M"
BASE COUNT          280 a          571 c          637 g          268 t
ORIGIN      
        1 accttccaca agcacgtcca ccagctgtgc aacggggtgc agattcacgt cgacgacccg
       61 gcctacgacc atgcggcgtt ccggccgtgg cggctgcagg cgctggggtt caaggcgatc
      121 cggcggctct atcccgacta cgcgctgtgg cgcgacttcg cgtatgaata cgtgttcgac
      181 aagctggcta tcgacgtgat caacggcggg tcggggctgc gcgaatgggt cgacgacccg
      241 gcctcgacgc cggccgatct ggaggcgctg accggaccgg acgaggcggc gtgggcggag
      301 cttcggaagc cgtacctgct gtactgaggc tagaaccggc gggcgatgcc gataccgaat
      361 tcgaggtcgg ggctgtcgtc gttgaggccg aggttgaggc cgaaatccag ctgtgcgtcg
      421 gtcatgaacg gcgggctcca ggcggcgagc aggtcgaagg tggcctgggt ggaggcgccc
      481 agcggatcgt cgtcgcggct gacccagact tcggccccca gcgaccagtc tccaacggga
      541 cggcccaagc cgacgacgag cgaccaggcg ccgtgcgagc cctcgccgtc gccgtcgcgg
      601 acgtattcga cgatgggcga cacgcccaga ccccagtcgc cgcccagatc gacagccacc
      661 ggcagggcga ggccgccttc gactccggag acgcggacct cttccccgcc cgtgggcagg
      721 gtggcgaagg cggtgacggc gaggccagtc ccgccgccgt ccgggcgcac gaacccgtgg
      781 cggacggcga ggaacagatc tccgaggcct tcggcgcgct gcgtcagccc ggtcgagcgg
      841 tctgtgacct tgtcgcgctg ccatgtggag aagcctgcct ggacttcggt cgaggggccg
      901 acgccgtagc ggacgacgac cgtcccggcg gcccagcttt cggcgcgggt cgcgtcgtcg
      961 tcctgccggg ccgtgtcgaa caggccgacc tcgacctgga catgaccctg atccatcaca
     1021 caggtggaat cgcccttgcc gggccggtcc ggacagaagg ggcgcaaagg cgcgtccgcg
     1081 gtctgggcgg ccgccggcgc cgccggcgcc agcgccagaa ccgcggccga agccgccaaa
     1141 gaccacatca ttcgcatcgc cccccgattg gcgtccgttg tagctacgcc tggatcagga
     1201 ggaaagtcgt cgctctactg agggtcgtac gttagccgta atcgccggct gttctccgca
     1261 aacgggggac gccatgatcg cactgttgtt tgcagctcta ctgacttttc aggagcctgc
     1321 gccgctgagc gcggacgctc gcgcgctgat cgcgccagtg gccgaggcga tcgccgcgga
     1381 ggaggcgagg caggctgcat tgccgccgcc ggccaatgat cgcgaaaagc tcgagcggat
     1441 gggcgtgctg gaccaggtcg ggcggcgggc gctgaccccg atcgatctga ctgtgttgcc
     1501 ggaggcggaa cgtgcagcag ccaacaccgc catgtgggcc ccgttgacgg caatggacga
     1561 gcggctgatg gcggagctcc tgaccatggt tccgcccgag ggctggttcc tgaagagcgt
     1621 ctacggtgat caggccgcca gcgccgcctt cctgatcgtg cagcattcgg atctggaaaa
     1681 ttggcggcgg ttcgtgccgg tgctggagcc gctggtggcg gctggcgagg tcaacgggca
     1741 gtcgtacgga ctgatg
//
LOCUS       DASVVN010000380         4459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_173887,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000380 DASVVN010000000
VERSION     DASVVN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173887"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..531)
                     /gene="ndk"
                     /locus_tag="VF686_06115"
     CDS             complement(112..531)
                     /gene="ndk"
                     /locus_tag="VF686_06115"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8661423.1"
                     /translation="MTERTFSIIKPDATRRNLTGAINAVIEGAGLRIVAQRRVKLTDA
                     QARKFYEVHAERPFYGELVGQMTAEPVVVQVLEGENAVARYREVMGATNPEQAAEGTI
                     RKQFALSIGENSVHGSDSIENAGIEIAQFFTDDQIVG"
     gene            676..2559
                     /locus_tag="VF686_06120"
     CDS             676..2559
                     /locus_tag="VF686_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX8661424.1"
                     /translation="MLQITDLTFNAWGRKFLEDASVSLPPGAKVGLVGRNGIGKSTLF
                     KLVLGELAAGGDEISLPKTARIGSVDQEHPATPVSVLETILEADEERHSLLGRLETAE
                     PEEMGEIWTRLIEIDADAAPARAAEILVGLGFSQENQQRPMSEYSGGWRMRVALAAAL
                     FAEPDMLLLDEPTNYLDLEGALWLEARLKKYPHTALIISHDREMLNEVCTHILHLANR
                     TLELYTGNYDQFEKARAEKARLQMSAKAKQDAERAHLQAFVDRFKAKASKAAQAQSRM
                     KRLAKMQPVATTIEERVAPFILPSPPRPLAPPLIRLERANVGYEPGRPILRNLNLRMD
                     LDDRIGLLGVNGAGKSTFAKMIAGALNVETGELHRDRKMRVGWFHQHQIEAMDPTDTP
                     LEIIRRAMPDAPESARRSKLAQWGLGYEKQETTVASLSGGERARLLLNQVAMDAPHIL
                     ILDEPTNHLDIDSRRALLDALNDYSGAVILITHDRSLMEMVADRLWLAADGTVKPYEG
                     DMDDYAKFVLDRAKMAAVKPSQIRDETAAAAPAQNGKKKNEKKSGPSPSTLRHAVKKA
                     EERMTQLTAEIARIDDDMANASVNNPKALEGLTRARAKTQADLDAAEAAWVAAEEALA
                     EVS"
     gene            3303..3545
                     /locus_tag="VF686_06125"
     CDS             3303..3545
                     /locus_tag="VF686_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661425.1"
                     /translation="MPKSRPPRPPAPPRADPELIRLVREAWPIIQWKKAISREINAMC
                     TGKGPWHITSDYYIRAGHNLIEKGILPPPGFPESQY"
     gene            complement(3606..>4459)
                     /locus_tag="VF686_06130"
     CDS             complement(3606..>4459)
                     /locus_tag="VF686_06130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8661426.1"
                     /translation="EIERRFLLPPGDGIAHYRYVIDLKATGRAPARVAARPAPRTPTR
                     SAERPLIYIDAGHGGRDPGARGANTNESAVTLAAALALKQELERTGRYRVRLTRESDA
                     YVDLYRRVAIARQADADLFISLHADAGSDPATRGASVYTLSEQGAGRAVREFTRGDNW
                     QRSLNLPGRDPSVDRILLDMTQRATQNRSAQLARTLLGELEGADHPMLRRSHRDAGLA
                     VLLAPDVPAVLLEMGFITNPEDERLLNDSRTRRRLMRAVAEGIDRYFREPAGGLQMAS
                     NGASGQP"
BASE COUNT          738 a         1580 c         1478 g          663 t
ORIGIN      
        1 gttgtgcgct atgcgcggct cgcggagcct cgcggcttga gcgcgcggcg aagccgccag
       61 gccgcgtcgg gtcgggaggt cccgaagggc cgacgaccgc ggcccaacaa attagccgac
      121 gatctggtcg tcggtgaaga actgcgcgat ctcgatgccg gcgttctcga tggagtccga
      181 accgtggacc gagttctcgc cgatcgacag ggcgaactgt ttgcggatcg tgccttcggc
      241 ggcctgttcc gggttggtgg cgcccatgac ctcgcgatag cgggccacgg cgttctcgcc
      301 ttccagcacc tgaaccacga ccggctcggc ggtcatctgg ccgaccaact cgccgtagaa
      361 cgggcgttcg gcgtgaactt catagaattt cctggcttgc gcgtcggtca gcttgacgcg
      421 gcgctgggcc acgatgcgca ggccggcgcc ctcgatcacg gcgttgatgg cgcccgtcag
      481 gttgcggcgc gtggcgtcgg gcttgatgat cgagaaggtg cgttcggtca tggaggtgac
      541 ttcttgttgg gagattgagg gtcgcgggct tatagcggcg ccctccccgg tttccaaccg
      601 ccacacaatt ttgcctcttt cgcgcgccgc aaggcggttc gatgcgtcag cggccgcccg
      661 agatcacgac cgaccatgct ccagatcacc gacctgacct tcaacgcctg ggggcgaaag
      721 ttccttgagg acgcttcggt cagcctgccg cccggcgcca aggtcggact ggtcggccgc
      781 aacggcatcg gcaagtcgac cctgttcaag ctggtcctcg gcgaactggc cgccggcggc
      841 gacgagatca gcctgcctaa gaccgcccgc atcggttcgg tcgatcagga acacccggcc
      901 acccccgtca gcgtgctcga aaccatcctg gaggccgacg aggagcgcca cagcctgctg
      961 ggccgcctcg aaaccgccga gcccgaggag atgggcgaga tctggacccg gctgatcgag
     1021 atcgacgccg acgccgcccc cgcccgcgcc gcagaaatcc tcgtcggcct cggcttcagc
     1081 caggagaacc agcagcggcc gatgtcggaa tactccggcg gctggcgcat gcgcgtcgcc
     1141 ctggccgcag cccttttcgc cgagccggac atgctgctcc tcgacgaacc gaccaactac
     1201 ctcgatctgg aaggcgccct gtggctcgag gcgcggctca agaaataccc gcacaccgcc
     1261 ctgatcatct cccacgaccg cgagatgctg aacgaggtct gcacccatat cctgcacctc
     1321 gcgaaccgga cgcttgagct ctacacgggt aactacgacc agttcgagaa ggcccgcgcc
     1381 gaaaaggccc gcctgcagat gtccgccaag gccaagcagg acgccgagcg cgcgcacctg
     1441 caggccttcg tcgaccgctt caaggccaag gcctccaagg ccgcccaggc ccagtcgcgt
     1501 atgaagcggc tggccaagat gcagcccgtg gccaccacca tcgaggaacg cgtcgccccc
     1561 ttcatcctgc cgtcgccgcc gcgtcctctg gcgccgccgc tgatccggct ggaacgggcc
     1621 aacgtcggct acgagccggg ccgcccgatc ctgcgcaatc tgaacctgcg catggatctg
     1681 gacgaccgca tcggcctgct gggcgtcaac ggggcgggca agtcgacctt cgccaagatg
     1741 atcgccggcg cgctgaacgt cgaaaccggc gaactgcacc gcgaccgcaa gatgcgcgtg
     1801 ggctggttcc accagcacca gatcgaggcc atggacccga cggacacccc gctggagatc
     1861 atccgccgcg ccatgcccga cgcgcccgag agcgcgcgcc gctcgaagct ggcccaatgg
     1921 ggtctgggct atgagaagca ggagaccacc gtcgccagcc tgtcgggcgg cgaacgcgcc
     1981 cgcctgctgc tcaatcaggt ggccatggac gcgccgcaca tcctgatcct cgacgaaccg
     2041 accaaccacc tggacatcga cagccgccgg gccctgctgg atgcgctgaa cgactattcc
     2101 ggcgccgtca tcctgatcac ccacgaccgc tcgctcatgg agatggtcgc cgaccggctg
     2161 tggctggccg ccgacggcac ggtgaagccc tatgagggcg acatggacga ctatgcgaaa
     2221 ttcgtcctcg accgcgcgaa gatggccgcg gtcaagccga gccagatcag ggacgagacg
     2281 gccgccgccg ctcccgccca gaacggcaag aagaagaacg aaaagaaatc aggcccttcc
     2341 ccctcgaccc tgcgccacgc agtgaagaag gccgaggagc gtatgaccca gttgaccgcc
     2401 gagatcgccc gcatcgacga cgacatggcc aacgcctcgg tcaacaaccc caaggccctc
     2461 gaaggcctga cccgcgcccg ggcgaagaca caggccgatc tggacgccgc cgaagccgcc
     2521 tgggtcgcgg cggaagaggc ccttgcagag gtgagctgag acaaagccgc ggaggcctct
     2581 cctccccact ccgtggaggg gcgcatccga agggatggag gggcgcatcc gccccctcat
     2641 ccgtgcatcc ccgcgaaggc ggggacccag gctttggcct caccgccggt gtcaggcgag
     2701 cgccggttcg ttcagacagc cttgtcgtcg ctcaaacctg ggtccccgcc ttcgcgggga
     2761 tgcacggtgt ttccgggcgc cggcaataaa cttccagcca tacagattac gaagcccaac
     2821 ccgttgatcc cgcacaggat caaacgataa ccagcgtcat ttcgtagcct tggtatcgcc
     2881 ccgtgcgaca ctcctccccg tcccgtcgcg ggggaggcgc aaaggccttg cgttctttgg
     2941 cggcgggatg tggagaagcg gggcccggtt cccggctgtc agagcaaggc ggccaacgcc
     3001 cggggccggt cggtcgcaca gtcgccgatc gggtttcagg gacgaggggg atactcgtcc
     3061 ggtccgggga ccgtgtcgca agcacggccc gcccgccgga ggcgctgcgg taaggcgata
     3121 agaccgccac cggacgcaag cttccgccaa atctccgcgc ggagcgtccg gcccgtggcc
     3181 gaaacggttc gtctcatctg atctccgggc ttcggtccgg cgctccgcac gccttcccgc
     3241 ccctcgcgcc gtccggcgcg gggccgtttc gcatgcctgc atgtcctgaa gaggagtccg
     3301 tcttgcccaa atcccgccct ccccggccgc cggccccgcc gcgcgccgat cccgaactga
     3361 tccgtctggt gcgcgaggcg tggccgatca tccagtggaa gaaggccatc agccgggaaa
     3421 tcaacgccat gtgcacgggc aagggccctt ggcacatcac ctcggactac tacatccggg
     3481 cggggcacaa tctgatcgag aaaggcatcc tgccgccgcc cggttttccg gagtcgcagt
     3541 attgagcgcc tccttgaatc cgtgcacccc cgcgaaggcg gggacgcacg ggagttctgg
     3601 gggccctagg gctgccccga cgccccgttc gaggccatct gcagcccgcc cgccggttcg
     3661 cggaaatagc ggtcgattcc ctcggccacg gcccgcatca ggcggcggcg ggtgcggctg
     3721 tcgttcagca gccgctcgtc ctcgggattg gtgatgaagc ccatctccag cagcacggcg
     3781 ggcacgtccg gggccagcag cacggccaga ccggcgtcgc gatggctgcg gcgcagcatc
     3841 gggtgatcgg cgccctccag ctcgcccagc agcgtgcggg ccagctgggc cgagcggttc
     3901 tgggtggcgc gctgggtcat gtcgagcagg atgcggtcga ccgacgggtc ccggcccggc
     3961 aggttcaggc tgcgctgcca gttgtcgccg cgcgtgaact cgcgcaccgc ccgccccgca
     4021 ccctgttcgg acagggtgta gacgctggcg ccgcgcgtcg ccggatcgga tccggcgtcg
     4081 gcgtgcaggg aaatgaacag atcggcgtcc gcctgacggg cgatggccac gcggcggtac
     4141 aggtcgacat aggcgtcgct ctcgcgcgtc agccgcacgc gatatcggcc agtccgctcc
     4201 agttcctgct tcagcgccag cgccgccgcc agcgtcacgg cgctctcgtt ggtgttcgcg
     4261 ccccgggccc cggggtcgcg gccgccatgt ccggcgtcga tgtagatcag cggccgttcg
     4321 gccgaccgcg tcggagtccg cggcgcgggc cgcgccgcca cacgggccgg cgctcggccg
     4381 gtggccttca ggtcgatcac atagcggtag tgggcgatgc cgtcgccggg cggcagcagg
     4441 aagcggcgct cgatctcgg
//
LOCUS       DASVVN010000381         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_199410,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000381 DASVVN010000000
VERSION     DASVVN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199410"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VF686_06135"
     CDS             complement(<1..621)
                     /locus_tag="VF686_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX8661427.1"
                     /translation="MSDTTGDTAIDARAPDRLTPLLFAVAIFTSASLVFVVQPMVTKL
                     VLPMLGGSPAVWNTAMVFFQTALLAGYGYAHGLQKIGSIKTQVAIHLALLVVAALFLP
                     LRVNGWLGDPDPSAPVMWLLATLALSVGAPFAVLSATAPLLQAWFARVRAGYPDGKNP
                     YVLYAASNLGSFLALLSYPFVIEPLTNLFGQRMGWTAGYWTFLLMVA"
     gene            705..1196
                     /locus_tag="VF686_06140"
     CDS             705..1196
                     /locus_tag="VF686_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8661428.1"
                     /translation="MFPDDIDALARQVVEAAVARGLMIATAESCTGGLVAGALTAVAG
                     SSAALDRGFVTYSNAAKTGMLGVAAGLIETHGAVSEPVARAMAEGAVSRSAATVSVAV
                     TGIAGPGGGSDEKPVGLVHFAASGPAGLIHAEHRFGDPGRETIRLESVRVALRLLLDR
                     IDA"
     gene            1193..1423
                     /locus_tag="VF686_06145"
     CDS             1193..1423
                     /locus_tag="VF686_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX8661429.1"
                     /translation="MIVDARGHRCPTPSLKLQKAMRDASAGAHLTLLATDPMARIDVP
                     HLMNGLGGRVVSVDEADGVLTIVVETPGVPTD"
     gene            complement(1386..>2004)
                     /locus_tag="VF686_06150"
     CDS             complement(1386..>2004)
                     /locus_tag="VF686_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX8661430.1"
                     /translation="VIGGYAAHRGFKALLAGLNLIHDEKEPHGFFKFNLLAFFVALAA
                     FLLFTVVSGAVVTSRLLEHAGPGEAPAASGLPLDGLWPALGLAIGLTMLYRYAMSHRT
                     PVAWAPSIAGGLVATVMSTISSWLCAIYVEQIAPLGATYGSVGAVVVLLIWLSWNVNA
                     IFYGGAFTTEMEIAARSIGAPEIEPDAPLPESVVNLSERRASRPR"
BASE COUNT          337 a          714 c          683 g          270 t
ORIGIN      
        1 cgccaccatc agcaggaagg tccagtagcc cgccgtccag cccatccgct gaccgaacag
       61 attggtcagc ggctcgatca cgaacggata ggacagcagc gccaggaagc tgccgaggtt
      121 ggacgcggca tagaggacgt aggggttctt gccgtcggga tagccggccc gcacccgcgc
      181 gaaccaggcc tgaagcaggg gcgcggtcgc cgacagcacg gcgaacggcg cgccgaccga
      241 cagggccagt gtcgccagca gccacatgac cggcgccgag ggatcgggat ctcccagcca
      301 gccgttgacc cgcagcggca gaaacagggc ggcgacgacc agcagcgcca gatggatcgc
      361 cacctgcgtc ttgatcgatc cgatcttctg cagcccgtgg gcatagccgt agcccgccag
      421 cagcgcggtc tggaagaaga ccatcgccgt gttccacacc gccggcgatc cgcccagcat
      481 cggcaggacc agcttggtca ccatcggctg gaccacgaac accagcgagg ccgaggtgaa
      541 gatggccacg gcgaacagca gcggcgtcag cctgtccggc gcgcgcgcgt cgatagccgt
      601 gtccccggtg gtgtcgctca tgcccgcccc gtatgttggc gccgttccgg gcgctttgcc
      661 ccaccctgaa ccgactcgca gccccttcgg aaggcccttg cccgatgttc cccgacgata
      721 ttgacgcact ggcccggcag gtcgtcgaag cggccgtcgc gcgcggcctg atgatcgcca
      781 cggccgagag ctgcaccggc ggcctggtgg cgggcgccct gacggccgtc gcgggctcgt
      841 cggcggcgct ggaccggggc ttcgtgacct acagcaacgc cgcgaagacc gggatgctgg
      901 gcgttgccgc cggcctgatc gagacccacg gggccgtgtc cgagccggtc gcccgggcca
      961 tggccgaggg cgccgtcagc cggtccgcag ccacggtgtc ggtcgccgtc acggggatcg
     1021 cgggtccggg aggtggatcc gacgaaaagc ccgtgggcct cgtccatttc gccgcctcgg
     1081 gccctgcggg cctgatccac gccgagcatc gcttcggcga tccgggccgc gagacgatcc
     1141 ggctggaaag cgtgcgcgtc gccctgcgcc tgttgctgga ccggatcgac gcatgatcgt
     1201 cgacgcccgc ggccaccgct gcccgacgcc cagtctgaag cttcagaagg cgatgcgtga
     1261 cgcttccgct ggcgcacatc tgaccctgct ggccaccgac cccatggccc ggatcgacgt
     1321 gccgcatctc atgaacggtc tgggaggccg cgtcgtttcg gtcgacgagg cggacggcgt
     1381 cctgactatc gtggtcgaga cgcccggcgt tccgacagat tgaccacgct ttccggcagg
     1441 ggcgcatcgg gctcgatctc gggcgcgccg atcgaccggg ccgcgatctc catctccgtg
     1501 gtgaaggcgc cgccgtagaa gatggcgttg acgttccacg acagccagat cagcagcacc
     1561 accaccgcgc ccaccgaacc gtatgtcgcc cccaggggcg cgatctgctc gacatagatg
     1621 gcgcacagcc atgacgagat cgtcgacatc accgtcgcca ccagcccgcc cgcgatggac
     1681 ggcgcccagg ccaccggcgt ccggtgggac atggcgtagc ggtagagcat ggtcaggccg
     1741 atcgcgaggc ccagcgcggg ccacagcccg tccagcggca ggccgctggc tgccggcgcc
     1801 tcgcccggac cggcatgctc cagcagccgc gaggtcacca ccgcacccga gaccacggtg
     1861 aacagcagga aggccgccag ggcgacgaag aaggccagca gattgaactt gaagaagcca
     1921 tgcggctcct tctcgtcatg gatcaggttc agcccggcca gcagggcctt gaagccgcga
     1981 tgggccgcgt atccgccgat gacc
//
LOCUS       DASVVN010000382         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_67952, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000382 DASVVN010000000
VERSION     DASVVN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67952"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VF686_06155"
     CDS             complement(<1..915)
                     /locus_tag="VF686_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8661431.1"
                     /translation="MASDPIRNIVVVGGGTAGWMAAAALSRLRANGVTQVTLIESDAI
                     GRVGVGEATIPPIRTFNAMLGIDEADFVKQTQGAFKLGIDFVGWTSPTHRYMHPFGAF
                     GADVEGVKFHQIWRMLHDQGKAGAFGEYNLCETAAWLNRFGPGDPRSPFTRMVWAYHF
                     DAALYARYLRNYSEARGVTRREGKVTGVEQDPETGFIKSVVMERGDPVEGDLFIDCTG
                     FRALLIEQTLKAGFEDWNHWLPCDRAVAVQCAHGDEPLTPYTRATARKAGWQWRIPLQ
                     HRIGTGYVYSSAHISDDEAAATLMANLDG"
     gene            1097..>2783
                     /locus_tag="VF686_06160"
     CDS             1097..>2783
                     /locus_tag="VF686_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661432.1"
                     /translation="MRFTTPLVRPEIARSATLKGGASLIAVAAMMAATSAFAQDPTPQ
                     TEATEVEEVVVTGIRGSLRSSQTIKRDAEVFVDSITSEDIGALPDRSVTEALQRVPGV
                     AIDRFAAGVDPDHFSVEGSGVVVRGLTFVRSELNGRDTFTANNGRALSFADVPPELMA
                     GVDVFKNPSADMIEGGIAGTVNLRTRVPFDSPGRVIAFSAEASYGDFAKKWTPTYSGL
                     ISDRWETEAGEFGLMLNAVYSQLISRGDGQQISNYGPRTLYSNGDVVTSPGATAVGPV
                     WFPRGAAFRSQSFDRERRGYAAAGQWRSNDRTMVATAQFLRSDSSQAWTEHAMEIATD
                     NVTNNGDSQAQQGTTLTFGPDGLFTNGIITGTTGWRADQNGPDARTPINGLQSNNIRR
                     DVNQNAVTTDYGLNFKWRATDRLGLTFDYQHVDSTVDVLDAGMWNSTYQNVSIDINGS
                     DIPLVTFLPPFINGTPCAGPPTAACPGYYRGANNNFSSPYNSFPRSAMDHKEASEGQL
                     DAFRVDADYSFEDGWIRSLRFGGRFAKREQTTRFSTYNWGVLSEQWGDGGPVWL"
BASE COUNT          489 a          934 c          901 g          459 t
ORIGIN      
        1 cccgtcgagg ttcgccatca gggtcgcggc cgcctcgtca tccgatatgt gggcgctgga
       61 atagacatag cccgtgccga tccggtgctg cagcgggatg cgccactgcc agcccgcctt
      121 gcgcgcggtg gcgcgggtgt agggggtcag gggctcgtcg ccgtgggcgc actggaccgc
      181 cacggcgcgg tcgcacggca gccagtggtt ccagtcctcg aacccggcct tcagcgtctg
      241 ttcgatcagc agtgcgcgga agccggtgca gtcgatgaac aggtcgccct cgacgggatc
      301 gccgcgctcc atgacgacag acttgatgaa gccggtctcg gggtcctgtt cgacgccggt
      361 taccttgcct tcgcgccggg tgacgccgcg cgcttcggaa tagttgcgca gatatctggc
      421 gtagagggcg gcgtcgaaat gataggccca gaccatgcgg gtgaacggcg accgcggatc
      481 ccccgggccg aagcggttca gccaggccgc ggtttcgcac agattgtatt caccgaaggc
      541 cccggccttc ccttggtcgt gcagcatgcg ccagatctgg tggaatttga cgccctcgac
      601 atcggccccg aacgcgccga acgggtgcat gtagcgatgg gtcggagagg tccagccgac
      661 gaagtcgatg cccagtttga aggcgccctg ggtctgtttg acgaagtccg cctcgtcgat
      721 gccgagcatg gcgttgaagg tgcggatcgg cgggatggtg gcctcgccca cgcccacccg
      781 tccgatcgca tcggactcga tcagggtgac ttgggtcacg ccgttggcgc gcagtcgcga
      841 gagggccgcg gcggccatcc agccggccgt tccacctccc acaacaacga tatttcgaat
      901 cggatccgac gccatttcac ttccccccgg agcgcgatct tcgccgcgtc tcatcgaaca
      961 atcctcttgc agaacaacga aatcaacggg cttgcgcact tccgaatgct gggatacgct
     1021 gattactcag acatttcacc gctgcacaat ggtagcgcta acatcaagaa atgctgagca
     1081 ggggaggttc aaggccatgc gttttaccac gccactcgtc cgtcctgaga tcgccaggag
     1141 cgccacgctc aaagggggcg catcgcttat cgccgtcgcc gccatgatgg cggcgacctc
     1201 ggcgttcgca caggatccga cgccgcagac ggaagccacc gaggtcgagg aagtcgtcgt
     1261 caccggcatc cgcggaagcc tgcgcagcag ccagacgata aagcgcgacg ccgaggtctt
     1321 cgtcgactcg atcacatccg aggatatcgg cgccctgccg gaccgctcgg tgaccgaggc
     1381 gctgcagcgc gtgcccggcg tggccatcga ccgtttcgcc gccggcgtcg atcccgatca
     1441 cttctcggtt gagggctcgg gggtggtcgt gcgcggcctg accttcgtcc gttccgagct
     1501 gaacggacgc gacaccttca ccgccaacaa cggccgggcc ctgagctttg cggacgttcc
     1561 gcccgagctg atggccggcg tcgacgtctt caagaaccct tcggccgaca tgatcgaggg
     1621 cggcatcgcc ggcacggtca atcttcggac gcgggtgccg ttcgactcgc ctggccgggt
     1681 catcgccttc tccgccgagg ccagctacgg cgatttcgcg aagaaatgga ccccgaccta
     1741 ttcgggcctg atcagcgacc gctgggagac cgaggccggc gagttcggcc tgatgctcaa
     1801 cgctgtctat tcgcagctga tctcgcgcgg cgacggacag cagatttcca actacggtcc
     1861 gcgcaccctg tattcgaacg gcgacgtggt cacgagcccc ggcgcgacgg cggtgggccc
     1921 ggtgtggttc ccgcgcggcg cggccttccg gtcgcagtcc ttcgaccgcg agcgccgcgg
     1981 ctatgcggcc gccggccaat ggcgcagcaa cgaccggacc atggtggcga cggcgcagtt
     2041 cctgcgctcc gactccagcc aggcctggac cgagcacgcg atggaaatcg cgaccgacaa
     2101 cgtgaccaac aacggcgact cccaggcgca gcagggcacg accctgacct tcggcccgga
     2161 cggcctgttc accaacggca tcatcaccgg caccaccggc tggcgggcgg accagaacgg
     2221 cccggatgcc cggacgccga tcaacggtct gcagagcaac aacatccgcc gcgacgtgaa
     2281 ccagaacgcg gtcaccaccg actatggcct gaacttcaag tggcgggcga ccgatcggct
     2341 ggggctgacg ttcgactacc agcatgtgga ttcgacggtc gacgtcctcg acgccgggat
     2401 gtggaacagc acttaccaga acgtctcgat cgacattaac ggcagcgata ttccgctggt
     2461 caccttcctg ccgccgttca tcaacggcac accctgcgcc ggaccgccga cggcggcctg
     2521 ccccggctac taccgcggcg ccaacaacaa cttctcgtcg ccgtacaaca gcttcccgcg
     2581 ctcggccatg gaccacaagg aggcgagcga gggtcagctc gacgccttcc gggtggatgc
     2641 ggactacagt ttcgaggatg gctggatccg ctcgctgcgg ttcggcggac gcttcgccaa
     2701 acgggaacag acgacgcggt tcagcaccta taactggggc gtcctgtccg aacagtgggg
     2761 cgacggcggt ccggtgtggc tga
//
LOCUS       DASVVN010000383         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_119948,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000383 DASVVN010000000
VERSION     DASVVN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119948"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VF686_06165"
     CDS             complement(<1..1046)
                     /locus_tag="VF686_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661433.1"
                     /translation="MNQTFVVTPRTGSAKAGAAERVRGFDEIHADFTPVAGEGQASRC
                     AQCGVPFCQTGCPLQNNIPDWLKLAAEGRAEEAWRQAEATSTLPEICGRICPQDRLCE
                     GNCTLEQAGWETVTIGSVERWLGDMAFENGWVDPIRPRVERSASVGIIGAGPAGMAAA
                     DRLRSAGYAVTIYDRHDRAGGLLTYGIPGFKLEKHVVQRRLDRLAEGGVEFRLGVDVG
                     GDLPFAEVRAAHDAVLVATGVYQARRLTAPGCGSEGVVAALDYLIASNRKGFGDAVPA
                     FDVGALDASGKRVVVIGGGDTAMDCVRTAVRQGAASVTCLYRRDRDNMPGSAREVVNA
                     EEEGVVFEWLAAPRA"
     gene            complement(1211..1888)
                     /locus_tag="VF686_06170"
     CDS             complement(1211..1888)
                     /locus_tag="VF686_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX8661434.1"
                     /translation="MRSAFACAVAAAALLTAGAASAQSAPEVAWNVGVVSDYVFRGYS
                     QTDEEPAIQGGVDATIGAFYVGAWASNVDFGDDTDAEVDVYGGFRGEAGGFAFDVGAV
                     GYGYVNAPDGADYDYLELKAAASRAVGPATFGGAVYWSPDFFGLDEEATYAEANVAFA
                     PADKWTVSGAIGHQWLDVNDDYATWNIGVGYAVTEHVAIDVRYHDADVDGPLSDGRAV
                     AGIKFLF"
     gene            complement(1981..3342)
                     /locus_tag="VF686_06175"
     CDS             complement(1981..3342)
                     /locus_tag="VF686_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8661435.1"
                     /translation="MTRLRIGLAGALTLGGISLAGTASAAPSAAPDPALLAHQTTLAL
                     DGAATGWILTSTALVLLMTLPGLALFYGGMVRRKNVIATITQSLGVASVVTLAWFIAG
                     YGLSFGVGQPIGAYTGDEVQSYIGSFQALFLNGVTPTTAHSLAPGLPEYLWIAYQLTF
                     AIITPALIAGAFAERIKYSGLLLFTGLWTLLVYAPICHWVWGGGFLGAAGVLDFAGGA
                     VVHVNAGVAGLVCAIFLGARKGYGKESIVAHNPVLTMIGASLLLVGWIGFNAGSAWTA
                     DAIASVAILNTILAAMAGSLTWKIVEIMEKRKPSLIGVLSGLIAGLVAITPAAGLVDP
                     KGAVIIGLASGPVCYAASVWIKKLLRYDDSLDAFGIHGAGGILGAVLTGVFASAAVNG
                     VAEGATVTKQIWGLGVTIGWSAVVTFLILIVCKFTTGLRVTAAQEAEGLDGHLHGEVM
                     EGA"
BASE COUNT          623 a         1225 c         1145 g          524 t
ORIGIN      
        1 gcgcggggcg cagcgagcca ttcgaagacc acgccctcct cctcggcgtt tacgacctcg
       61 cgagcgctgc ccggcatatt gtcccggtcg cggcggtaca ggcaggtcac cgacgccgcg
      121 ccctgccgca cggccgtgcg gacgcagtcc atcgccgtgt cgccgccgcc gatcacgacc
      181 acccgctttc ccgatgcgtc cagggccccg acgtcgaagg ccggcacggc gtcgccgaag
      241 cccttgcggt tcgaggcgat cagataatcc agcgccgcga cgacgccttc ggagccgcag
      301 cccggcgcgg tcagccgccg cgcctgatag accccggtcg ccaccagaac tgcgtcatgc
      361 gccgcacgaa cttcggcgaa cggcagatca ccaccgacat cgacgccaag tcggaactcg
      421 accccgccct cggccagccg gtccagccgg cgctggacca catgcttctc gagcttgaag
      481 ccggggatgc cataggtcag cagaccgccc gcgcggtcgt gccggtcgta tatcgtcacc
      541 gcatagcccg cagaccgcag ccggtccgcc gccgccattc ccgccggtcc ggcgccgatg
      601 attccgaccg aggcgctgcg ctcgacccgc ggccggatcg gatcgaccca gccgttctcg
      661 aacgccatgt cgcccagcca gcgctcgacc gagccgatgg tcaccgtctc ccagcccgcc
      721 tgctccaggg tgcagttgcc ttcgcacagc cggtcctgcg gacagatgcg gccgcagatt
      781 tcgggcaggg tcgaggtcgc ctcggcctgc cgccacgcct cctcggctcg gccttcggcg
      841 gccagcttca gccagtcggg gatgttgttc tgcagcgggc aaccggtctg gcagaagggc
      901 accccgcact gggcgcagcg gctggcctgt ccctcccccg cgacaggcgt gaagtccgca
      961 tggatttcgt cgaacccgcg cacgcgctcg gccgcgccgg ccttggccga gccggtccgc
     1021 ggagtgacga caaaagtctg gttcaccgac gccacatcga tcctttcgct aacgcacagg
     1081 acttagaggc gggagtgaat gctggcaact tccgtgcaac atatgcattg aaagacgacc
     1141 agcacgcaaa aactcaccgg tcagacgcaa tcaacgacaa cgtccgaccg gtctggtttg
     1201 tgcaacgcat tcagaacagg aacttgattc cggctacagc gcggccgtcc gacaggggtc
     1261 cgtccacgtc cgcgtcgtga tagcggacgt cgatcgccac atgttcggtg acggcgtaac
     1321 cgaccccgat attccaggtc gcatagtcgt cgttcacgtc cagccactga tggccgatcg
     1381 cgcctgatac ggtccatttg tcggccggcg caaaggcgac gttggcctcg gcgtaggtcg
     1441 cctcctcgtc cagcccgaag aagtcgggcg accagtagac ggcgccgccg aacgtcgccg
     1501 ggccgaccgc gcgcgacgcc gccgccttga gctcgagata gtcgtagtcg gccccgtctg
     1561 gcgcgttgac atagccatag ccgaccgccc cgacgtcaaa ggcgaaaccg ccggcttcgc
     1621 cgcggaagcc gccgtagacg tccacctcgg catcggtgtc atcgccgaaa tcgacgttgg
     1681 aggcccaggc cccgacgtag aaggccccga tcgtggcgtc gacgccgccc tggatggcag
     1741 gctcctcgtc ggtctggctg tagccgcgga agacatagtc gctgaccacg cccacgttcc
     1801 aggccacctc gggcgcggac tgggcgctgg cggccccggc ggtcagcagg gcggccgcag
     1861 cgaccgcaca ggcgaaagct gatctcatca tcgtatcccc ttgttatcga tggttgttcg
     1921 agactcacgc ctactgggcg gcgacgcgga ggggaggctc cgcgccgcct gtggttcgga
     1981 tcaggcgccc tccatcacct cgccgtgcag atggccgtcc agcccctcgg cctcctgcgc
     2041 cgcggtgacg cgcaggccgg tggtgaactt gcagacgatc aggatcagga aggtgaccac
     2101 ggcgctccag ccgatggtga cgcccaggcc ccagatctgt ttggtgacgg tcgcgccttc
     2161 cgccacgccg ttgacggcgg ccgaggcgaa gacaccggtc agcacggcgc cgaggatgcc
     2221 gcccgccccg tggatgccga aggcgtccag gctgtcgtca tagcgcagca gcttcttgat
     2281 ccagaccgag gccgcatagc agaccggacc cgaggccagg ccgatgatca cagctccctt
     2341 gggatcgacc agaccagccg ccggcgtgat ggcgaccagg ccggcgatca ggccggacag
     2401 cacgccgatc agcgacggtt tccgcttctc catgatctcc acgatcttcc aggtcagcga
     2461 accggccatg gcggccagga tggtgttcag gatggcgacg gaggcgatcg cgtccgcagt
     2521 ccaggccgag ccggcgttga agccgatcca gccgaccagc agcagcgagg cgccgatcat
     2581 cgtcagcacc ggattgtggg cgacgatgct ctccttgccg tagcccttcc gcgcgccaag
     2641 gaagatggcg cagaccagac cggccacgcc cgcgttgacg tgcacgacgg cgccgcccgc
     2701 gaagtccagg acgcccgccg cgcccaggaa gccgccgccc cagacccagt ggcagatcgg
     2761 ggcgtagacc agcagcgtcc acagcccggt gaacagcagc aggcccgaat atttgatccg
     2821 ttccgcgaac gcccccgcga tcagcgccgg cgtgatgatg gcgaaggtca gctggtaggc
     2881 gatccagaga tattcgggca gaccgggagc caggctgtgc gccgtggtcg gcgtcacgcc
     2941 gttcaggaac agcgcctgga acgagccgat gtagctctgc acctcgtcgc cggtataggc
     3001 gccgatcggc tggccgacgc cgaacgacag gccatagccc gcaatgaacc acgccagggt
     3061 gaccacggag gccacgccca gcgactgggt gatggtggcg atgacgtttt tgcgccggac
     3121 catgccgccg tagaacaggg cgagacccgg cagggtcatc agcaggacca aggcggtcga
     3181 tgtcaggatc cagcccgtcg ccgcgccgtc gagggcgagt gtcgtctggt gcgccagaag
     3241 cgcggggtcc ggagcggcgg acggggcggc ggaagccgtc cccgccagac tgatcccccc
     3301 aagggtcaga gcccccgcaa gtccgatacg aagtcgtgtc atttgatccc ccaggacagc
     3361 gttacgcatg tgcgaaatgt ttaccgcatt tgcgagatag acaagtgggc ggacggtgtt
     3421 ttgcgggtaa tttgtgcgca tagaacgaga acgtctacga tttcggcaag ttacttgctc
     3481 aaaagcatga gccaacggta actggcgatg aggggtc
//
LOCUS       DASVVN010000384         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_360425,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000384 DASVVN010000000
VERSION     DASVVN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360425"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="VF686_06180"
     CDS             <1..158
                     /locus_tag="VF686_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661436.1"
                     /translation="GLIGASQLEASTVDLSAEFTGLITTQRAYSASSKIITTADEMLA
                     ELISIKR"
     gene            322..609
                     /locus_tag="VF686_06185"
     CDS             322..609
                     /locus_tag="VF686_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX8661437.1"
                     /translation="MLQERRLNSRGEQYVVGPTGTPLTMHDLPPANTDRWVIRRKAEV
                     VAAVRGGLISLDDALARYRLTAEEFLAWQKAIDKWGMQGLRTTRIQHYGRP"
     gene            727..>1673
                     /gene="mnmA"
                     /locus_tag="VF686_06190"
     CDS             727..>1673
                     /gene="mnmA"
                     /locus_tag="VF686_06190"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX8661438.1"
                     /translation="MTLAEDICPVPNIAPMTGQADMDAAIESARAAVGLPVGSRVVAA
                     MSGGVDSTVVAGLLHKAGYDVVGVTLQLYDHGAALKKKGACCAGQDIHDARLAAETLG
                     IPHYVLDYESRFRDAVIDQFADSYLAGQTPVPCIRCNQTVKFRDLLDVARDLGAEAMA
                     TGHYVKRAVVDGRSQMRKAIDHSRDQSYFLFATTAQQLDYLRFPLADLEKPQVRGVAL
                     ELGLRIAAKPDSQDICFVPNGDYRTLIDKLRPQGREAGDIVHMDGRVLGAHSGITDYT
                     IGQRRGLNVAVGEPLFVTKLDPAKRHVIVGPREALLTASL"
BASE COUNT          291 a          570 c          533 g          279 t
ORIGIN      
        1 ccggcctgat cggagcgtcg cagctggaag cctcaaccgt cgatctctcg gccgagttca
       61 ccggcctcat caccacccaa cgcgcctatt cggcatcgtc caagatcatc accacggccg
      121 acgagatgct ggccgaactg atcagcatca aacgctgatc tctgagtaac gggccgtaaa
      181 cctcaatgaa tcctttcttg aatattgagc ctgtggacgg agggcatttt aggctttcct
      241 tcaccacgac gcttaaacgg cccttagtcg gcgaccgcta ccgttcactc atagtggtgg
      301 tggtcaatga ggcataagcc catgttgcaa gagcgacgtc tgaatagccg aggcgaacaa
      361 tatgtggtgg gcccgacggg cacgccgctg acgatgcacg atcttccccc ggccaatacc
      421 gaccgctggg tgatccgtcg caaggccgag gtcgtggccg ccgtgcgtgg cggtctgatc
      481 agcctggatg acgccctggc gcgttaccgt cttacggccg aggagttcct ggcgtggcag
      541 aaggccatcg acaaatgggg catgcagggc ctgcgcacga cgcggatcca gcactacggc
      601 cgcccgtaaa tcttctcccc gagccgaccg cggccgcttc cggaaacgga ggcggccgtt
      661 gtcgtttccg gccctctttt cgccccggcc tcgaaatcga acgggtttcg cactagatag
      721 cgcgccatga cgctcgccga ggacatctgc cccgtcccga acatcgcccc catgaccggc
      781 caggccgaca tggacgcggc gatcgagtcc gcgcgcgccg ccgtcggcct gccggtcgga
      841 tcccgggttg tcgccgccat gtcgggcggg gtggactcca ccgtggtcgc cggcctgctg
      901 cacaaggcgg gttatgacgt cgtcggcgtg accctgcagc tgtacgacca cggcgcggcg
      961 ctgaagaaga agggcgcctg ctgcgccggt caggatatcc acgacgcccg gctggcggcg
     1021 gagacgctgg gcatccccca ctacgtcctc gactacgaaa gccggttccg ggacgcggtc
     1081 atcgaccagt tcgccgacag ctatctggcc ggccagacgc cggtgccctg catccgctgc
     1141 aaccagacgg tcaaattccg cgacctgctg gacgtcgccc gcgatctggg cgccgaggcc
     1201 atggcgaccg gccattacgt caagcgcgcg gtcgtcgacg gccgctcgca gatgcgcaag
     1261 gcgatcgacc attcgcgcga tcagtcctac ttcctgttcg ccactacggc gcagcagctg
     1321 gactatctgc gcttcccgct ggccgatctg gagaagccgc aggtgcgggg cgtggcgctg
     1381 gaactgggcc tgcggatcgc cgccaagccg gacagccagg acatctgctt cgtccccaac
     1441 ggcgactacc gcaccctgat cgacaagctg cgtccgcagg gccgcgaggc cggcgacatc
     1501 gtgcacatgg acgggcgggt gctgggcgcg cactcgggca tcaccgacta caccatcggc
     1561 cagcgccggg gactgaacgt cgccgtgggc gagccgctgt tcgtgacgaa gctggatccg
     1621 gcgaaacgcc acgtcatcgt cggtccgcgg gaggcattgc tgaccgcgtc gct
//
LOCUS       DASVVN010000385         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_385594,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000385 DASVVN010000000
VERSION     DASVVN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385594"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VF686_06195"
     CDS             complement(<1..1154)
                     /locus_tag="VF686_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8661439.1"
                     /translation="MGTSAHIDRFVLDHLPPADQQPELVFDLPELAYPERLNAGVELL
                     RRAVEAAGPDARALIDHDHEFSWSRVDAVSGRMARVLVEEMGLVPGNRVLLHGPNSSW
                     TVMAWFAILKAGGVVVATMPMLRAAELATVIDKAQISHALVYAALADAVTEAQGATTT
                     LTQVMDSAALQKGAQALPAGFRFDAVDTAADDPALIAFTSGTTGKPKGCVHFHRDILA
                     MADTFARRTLDLAPGDIVVGTPPIAFTFGLGGLVVFPAAVGAATAFANRPGFDALAET
                     VARHGATAMFTAPTGYRALLKLAGDHDLSSLRTCVSAGEALPAATSDAWHEATGVRII
                     DGIGSTEMIHIFISAAGEDIRPGATGKPVPGYRARIQDEAGNPLPLGETGR"
     gene            complement(1154..>2249)
                     /locus_tag="VF686_06200"
     CDS             complement(1154..>2249)
                     /locus_tag="VF686_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8661440.1"
                     /translation="ANEDVLHLHGDHDLDGDCRTILKALAAGGWLKNAVPDAEGKLDV
                     RTLCLTRETLARHSGLADFVFAMQGLGSGPISLFGTDEQKARWLPGVAAGEIIAAFAL
                     SEPDAGSDVAALSTSARPDGDARNGGWVLDGVKTFISNGGLADRYTVFARTGDGSGAK
                     GISAFVVDADTPGFEIVERIPLMAAHPLATIRFNGCRIDGDRILGAPGDGFRIAMATL
                     DVFRSTVAAAALGFSRRAFEEAASRTVARKLFGAPMSDLQLVQASLADMALKIDASAL
                     LIYRAAWLKDQGAPRVTREAAMAKLYATDEAQQVIDQAVQLFGGLGVVSGHPVERLYR
                     EIRALRIYEGASEVQKIVIARQVLAGLGGL"
BASE COUNT          328 a          757 c          847 g          317 t
ORIGIN      
        1 cggccggtct cgccgagcgg cagcggattg cccgcctcgt cctggatgcg ggcgcggtag
       61 ccgggcacgg gcttgccggt ggcgccgggg cggatgtcct cgcctgccgc cgagatgaag
      121 atgtggatca tctcggtcga gccgatgccg tcgatgatgc gcacgccggt ggcctcatgc
      181 catgcgtcgg acgttgccgc gggcagggcc tcgccggccg agacgcaggt gcgcagggag
      241 gacaggtcgt ggtcgccggc cagtttcagc agggcgcggt agccggtcgg ggcggtaaac
      301 atggcggtgg cgccatggcg ggcgacggtc tcggccaagg cgtcgaaacc cgggcggttg
      361 gcgaaggccg tcgcggcgcc gacggcggcc gggaagacca cgaggcctcc gaggccgaag
      421 gtgaaggcga tcggcggggt gccgacgacg atgtctccgg gcgcgaggtc cagtgtgcgc
      481 cgggcgaagg tgtcggccat ggcgaggatg tcgcgatgga aatggacgca gcccttgggc
      541 ttgcccgtcg tacccgaggt gaaggcgatc agggcgggat cgtcggcggc cgtgtccacg
      601 gcgtcgaaac ggaagccggc gggcagggcc tgcgcgccct tctgtagcgc ggccgaatcc
      661 atcacctgcg tcagcgtggt ggtcgccccc tgggcctcgg tcaccgcatc ggcgagggcc
      721 gcatagacca gcgcgtggct gatctgcgcc ttgtcgatga ccgtcgccag ttcggcggcc
      781 cggagcatgg gcatggtggc gacgaccacg ccgccggcct tcaggatcgc gaaccacgcc
      841 atcaccgtcc aggatgagtt cggtccgtgc agcagcacgc ggttgccggg gaccagcccc
      901 atctcctcga ccagcacccg cgccatccgg cccgagacgg cgtcgacgcg gctccacgag
      961 aactcatggt cgtgatcgat cagggcgcgg gcgtcgggac cggccgcctc gacggcccgg
     1021 cgcagcagtt cgaccccggc gttcagccgc tcggggtagg ccagttccgg caggtcgaag
     1081 accagctccg gctgctggtc cgccggcggc aggtggtcga ggacgaaccg gtcgatatgg
     1141 gcgctggtcc ccatcagagg cctcccagac ccgccagcac ctgccgggcg atgacgatct
     1201 tctggacctc cgacgcgccc tcgtagatgc gcagcgcccg gatctcgcgg tacagccgct
     1261 cgaccggatg gcccgagacg acgccgaggc cgccgaacag ctggacagcc tgatcgatca
     1321 cctgctgcgc ctcgtcggtg gcgtagagtt tggccatggc ggcctcgcgg gtgacacgcg
     1381 gcgcgccctg atccttcagc caggcggcgc ggtagatcag cagggccgag gcgtcgatct
     1441 tcagcgccat gtcggccagc gaggcctgca ccagctgcag gtcgctcatc ggcgcgccga
     1501 acagcttgcg ggcgacggtg cgtgacgcag cctcttcaaa ggcccggcgg gagaagccga
     1561 gagcggcggc ggcgaccgtg gagcggaaga cgtccagcgt ggccatggcg atgcggaaac
     1621 cgtcgcccgg cgcgcccagg atccggtcgc cgtcgatgcg gcagccgttg aaccggatcg
     1681 tcgccagcgg gtgggcggcc atcagcggga tgcgttcgac gatctcgaag ccgggcgtgt
     1741 cggcgtcgac gacgaaggcg ctgatgccct tggcgccgct cccgtcgccg gtgcgggcga
     1801 agacggtgta gcggtcagcg aggccgccgt tggagatgaa ggtcttcacg ccgtccagca
     1861 cccagccccc gtttctggcg tcgccatccg ggcgcgcgct ggtcgacagg gcggcgacgt
     1921 cggagccggc gtcgggctcc gacaaggcga aggcggcgat aatctcgccc gcggccacgc
     1981 cgggcagcca gcgggccttc tgctcatccg tcccgaacag gctgatcggg ccggaaccca
     2041 gcccctgcat ggcgaagacg aagtcggcga ggcccgaatg gcgggcgagg gtctcgcgcg
     2101 tcaggcacag ggtgcgcacg tccagctttc cctcggcgtc gggcaccgcg tttttcagcc
     2161 agccgcccgc cgccagcgcc ttcaggatcg tgcggcagtc gccgtccaga tcgtggtcgc
     2221 cgtggagatg cagcacgtct tcgttcgct
//
LOCUS       DASVVN010000386         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_68066, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000386 DASVVN010000000
VERSION     DASVVN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68066"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1817)
                     /locus_tag="VF686_06205"
     CDS             complement(<1..>1817)
                     /locus_tag="VF686_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX8661441.1"
                     /translation="VGMIFLPRIDLAAQERARTIVEREVMAAGFWLYGWRQVPTHVSV
                     LGQRAAATRPEIEQIMLSPPAGLDGEELERALYLLRRRIEARATAAGLQHFYVCSLSA
                     RSVIYKGMMLAQALADFYPDLNDPRVEANVALFHQRYSTNTFPEWRLAQPFRMLAHNG
                     EINTLRGNAGWMRSHEIRMTAEAFGADDATVKPVIQAGGSDSAALDNVFEVLVRAGRP
                     APKVKALLITEAPIEGVISDDHRALYAYCNAVMEPWDGPAAICATDGRWVVAGKDRNG
                     LRPLRVAETVDGLLICGSEVGMTGIADDRIKRRLHIGGGRMISVDLEAGVLYDEIQTV
                     DRLAAALPYRQWLSNMVELEPLVGPGPEPRRLSGEALTRFQAAVGWTREDIDTLLDPM
                     AGEGKEAVASMGDDAPPAVLSHQDRPFAHYFRQAFAQVTNPPIDSLREAGAMSLRTRF
                     KNLGNILAQDETQTQVFVLDSPVLTTGMYERMLDVVGAGATTVIDCTFPAGEGALKHA
                     LDRIRDEAVAAAKGGAGLIVLTDERMGEGRPLISMILATAAVHGRLVDEGLRTFCSLV
                     VRAAEAIDAHTFAVLVGCGATAVNGWLAQETFLERLTGG"
BASE COUNT          288 a          664 c          589 g          276 t
ORIGIN      
        1 gcccgcccgt cagccgctcg agaaaggttt cctgcgccag ccagccgttg accgccgtgg
       61 ccccgcatcc gaccaggacc gcgaacgtat gggcgtcgat cgcctcggcc gcgcgcacca
      121 ccagcgagca gaaggtgcgc agcccctcgt cgaccagccg gccgtgcacg gcggccgtgg
      181 ccagaatcat ggaaattagc ggccggccct cgcccatccg ttcgtcggtc agcacgatca
      241 agcccgcgcc gcccttcgcc gccgccacgg cctcgtcgcg gatgcgatcc agcgcgtgtt
      301 tcagcgcgcc ctccccggcc gggaaggtgc agtcgatgac cgtagtcgcc ccggccccga
      361 ccacgtccag catccgctcg tacatgccgg tcgtcagaac cggactgtcc agcacgaaga
      421 cctgcgtctg ggtctcgtcc tgggcgagga tgttgcccag gttcttgaac cgcgtccgca
      481 ggctcatcgc ccccgcttca cgcagggagt cgatcggcgg gttggtgacc tgggcgaagg
      541 cctggcggaa atagtgggcg aagggccgat cctgatgcga cagcaccgcc ggcggcgcgt
      601 cgtcgcccat ggaggcgacg gcctccttgc cctccccggc catcggatcc agcagtgtgt
      661 cgatgtcctc ccgggtccag ccgacggcgg cctgaaaacg ggtcagcgcc tcgcccgaca
      721 ggcggcgcgg ttcgggcccg gggccgacca gcggctccag ctcgaccatg ttcgacaacc
      781 attggcgata gggcagcgcg gcggccaacc ggtcgacggt ctggatctcg tcgtagagaa
      841 cgcccgcctc cagatcgacc gagatcatcc gtccgccgcc gatgtgcagg cggcgcttga
      901 tccggtcgtc cgcgatcccg gtcattccga cttccgaacc gcagatcagc aggccgtcga
      961 ccgtttcagc cactcgcaat ggacgcaggc cgttgcggtc cttgccagcc accacccagc
     1021 ggccgtccgt ggcgcagatc gccgcgggac catcccatgg ctccatgacc gcgttgcagt
     1081 aggcgtagag ggcgcggtgg tcgtcgctga tcacaccctc gatcggcgcc tcggtaatga
     1141 gcagggcctt gaccttgggc gcgggccggc ccgcgcggac cagaacctcg aacacattgt
     1201 ccagcgccgc cgagtccgaa ccgccggcct ggatcaccgg cttgaccgtc gcgtcgtcgg
     1261 cgccgaaggc ctcggcggtc atgcggattt cgtgactgcg catccacccg gcgttcccgc
     1321 ggagggtgtt gatctcgccg ttgtgggcca gcatccggaa cggctgggcc agccgccatt
     1381 ccggaaaggt gttggtcgaa tagcgttgat ggaacaaggc cacattggcc tcgacccgcg
     1441 ggtcgttcag atccggatag aagtccgcca gcgcctgggc cagcatcatc cccttgtaga
     1501 tcaccgaccg cgccgacagc gagcagacgt agaaatgctg cagtcccgcc gccgtcgccc
     1561 gcgcctcgat ccgtcgccgc agcaggtaca acgcccgctc cagttcctcg ccgtccaagc
     1621 ccgccggcgg gctgagcatg atctgctcga tctcaggccg ggtcgcggcc gcgcgctgac
     1681 ccagcaccga cacatgggtc ggcacctgac gccagccgta aagccagaag ccggccgcca
     1741 tgacctcgcg ctcgacgatg gtgcgggccc gttcctgcgc cgccagatcg atgcgcggca
     1801 ggaagatcat gccgacg
//
LOCUS       DASVVN010000387         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_120152,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000387 DASVVN010000000
VERSION     DASVVN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120152"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="coxB"
                     /locus_tag="VF686_06210"
     CDS             <1..907
                     /gene="coxB"
                     /locus_tag="VF686_06210"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8661442.1"
                     /translation="SPLKVQAHFFHDAVLMPVITFITLLVLGLSIWIVVRYNKKSNPV
                     PAKWSHNTLVEVIWTVLPVLILVFISLFSFRLLFAYHDMPTPDLTVKATGNQWNWAYE
                     YPDQGIAEYVSNMLPEDELAERGMPHSLYRLAADEPIVVPVGKTVRVLVTAADVIHAF
                     ALPAFGLKTDAVPGRVNETWFRADRVGTFYGQCSELCGVDHAFMPIQINVVTQAEFEA
                     WVASKGGSMTPKTPEQAPGGTAAQVAAGTTGAIASRNVSSEAMRPAATAPAGQPPADT
                     GAVDGTPSARSAPAIPDTAAAPAAQ"
     gene            931..2601
                     /gene="ctaD"
                     /locus_tag="VF686_06215"
     CDS             931..2601
                     /gene="ctaD"
                     /locus_tag="VF686_06215"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX8661443.1"
                     /translation="MATAAENLAYDHDGHDDHDHKPGFFTRWFLSTNHKDIGTLYLLF
                     AIMAGLVGGILSGFIRLELAEPGIQYFMEGGLIDKLGLVEASKHGYNVTVTAHALIMI
                     FFMVMPAMIGGFGNWFVPIMIGAPDMAFPRMNNVSFWLLVAAWVLLCLSMFVDGGPGK
                     GFGGGWTIYPPLSTTGHTGPAMDLAIFSLHVAGASSILGAINFITTIFNMRAPGMTLH
                     RMPLFAWSVLITAFLLLLSLPVLAGAITMLLADRNFGTSFFDPAGGGDPVMFQHLFWF
                     FGHPEVYILILPGFGIVSHIVSTFSKKPIFGYLAMAYAMVAIGFVGFIVWAHHMYTVG
                     MSVNLRAYFIAATMIIAVPTGVKIFSWIATMWGGSISFKTPMLWAIGFIFLFTVGGVT
                     GVVLSNAGIDYSLHDTYYVVAHFHYVLSLGAVFAIFGGFYYWYEKMFGVKYNEFLGCA
                     HFWIMFVGVNLVFFPQHFLGLQGMPRRYVDYADGYAYWNMISSIGYAITAVGVLVFLV
                     MLAESAIRRRKGVANPWGEGATTLEWTLSSPPPHHQFNELPVVKSDDH"
     gene            2681..3649
                     /gene="cyoE"
                     /locus_tag="VF686_06220"
     CDS             2681..3649
                     /gene="cyoE"
                     /locus_tag="VF686_06220"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX8661444.1"
                     /translation="MTETTPQPAISMAQPEDFFQLLKPRVMSLVVFTGATGLVVAKGG
                     IDWISAAIAILCIAVGAGAAGALNMALEGETDALMRRTRGRPVAAGRVRKNDAMAFGV
                     ILSIFSVMLLGMTTNWLAGGLLLLTIVYYAAFYTLLLKRRTPQNIVIGGAAGAFPPVI
                     GWAAATGTAPWQAWLLFLIIFLWTPPHSWALALYSAGDYAKAGIPMMPVARGAKSTRL
                     QILLYTLIFVPVAIAPGLTGLGGLLYLAVSILGGLGFLFLAVRLYRSRAGDQPDKAEA
                     VGREAALYDVRAEAKPARNLFAFSILYLMALFAALLVESVAAIPGL"
BASE COUNT          567 a         1306 c         1158 g          688 t
ORIGIN      
        1 gtcgccgctg aaggtccagg cgcacttctt ccatgacgcc gtgctgatgc cggtgatcac
       61 cttcatcacc ctgctggtgc tgggcctgag tatctggatc gtcgtccgct acaacaagaa
      121 gtccaacccg gttccggcca agtggagcca caacaccctg gtcgaggtga tctggaccgt
      181 ccttcccgtc ctgatcctgg tgttcatctc gctgttctcg ttccgcctgc tgttcgccta
      241 tcacgacatg ccgacgccgg acctgacggt gaaggccacg ggcaaccagt ggaactgggc
      301 ctatgagtat cccgaccagg gcatcgccga atacgtctcg aacatgctgc cggaagacga
      361 gctggccgag cgcggcatgc cgcacagcct gtaccgcctg gccgccgacg agccgatcgt
      421 ggtgccggtc ggcaagaccg tccgcgtgtt ggtcaccgcc gccgacgtca tccacgcctt
      481 cgccctgccg gccttcggtc tgaagaccga cgccgtcccc ggccgcgtca acgagacctg
      541 gttccgcgcc gaccgcgtcg gcaccttcta cggccagtgc tcggaactgt gcggcgtcga
      601 ccacgccttc atgccgatcc agatcaatgt cgtcacccag gccgagttcg aagcctgggt
      661 cgcctccaag ggcggctcga tgacgccgaa gactccggaa caggctccgg gcggcaccgc
      721 cgcccaggtc gccgccggca cgacgggcgc gatcgcctcg cgcaacgtct cgtcggaggc
      781 gatgcggccc gctgcgaccg cccccgctgg acagcccccg gcagacaccg gcgccgtcga
      841 cggcacgccg tcggcccgct ccgcccccgc cattcccgac accgccgccg caccggcggc
      901 ccagtaaaga tccgcgagag cccccagatc atggccaccg cagccgaaaa cctcgcttat
      961 gaccacgacg gtcatgacga ccacgaccac aagccgggct tcttcacccg ctggttcctg
     1021 tcaaccaacc acaaggacat cgggacgctg tacctgctgt tcgccatcat ggcgggcctg
     1081 gtcggcggca tcctgtccgg cttcatccgc ctcgagctgg cggaaccggg catccagtac
     1141 ttcatggaag gcgggctgat cgacaagctc ggcctcgtcg aggcgtccaa gcacggctac
     1201 aacgtcaccg tcacggccca cgccctgatc atgatcttct tcatggtcat gccggcgatg
     1261 atcggcggct tcggcaactg gttcgttccg atcatgatcg gcgcgccgga catggccttc
     1321 ccgcgcatga acaacgtctc gttctggctg ctggtcgcgg cctgggtgct gctgtgcctg
     1381 tcgatgttcg tcgacggcgg cccgggcaag ggcttcggcg gcggctggac catctatccg
     1441 ccgctgtcca cgaccggcca caccggcccg gccatggatc tggcgatctt ctcgctgcac
     1501 gtcgcgggcg ccagctcgat cctcggcgcg atcaacttca tcaccaccat cttcaacatg
     1561 cgcgcgccgg gcatgaccct gcaccggatg ccgctgttcg cctggtcggt gctgatcacc
     1621 gccttcctgc tgctgctgtc gctgcccgtg ctggccgggg ccatcaccat gctgctggcc
     1681 gaccgcaact tcggcaccag cttcttcgac ccggccggcg gcggcgaccc cgtcatgttc
     1741 cagcacctgt tctggttctt cggccacccg gaggtctaca tcctgatcct gccgggcttc
     1801 ggcatcgtca gccacatcgt ctcgaccttc tcgaaaaagc cgatcttcgg ctacctcgcg
     1861 atggcctacg ccatggtcgc catcggcttc gtcggcttca tcgtgtgggc gcaccacatg
     1921 tacacggtcg gcatgagcgt gaacctgcgc gcctatttca tcgccgcgac gatgatcatc
     1981 gcggtgccga cgggcgtgaa gatcttcagc tggatcgcca cgatgtgggg cggctcgatc
     2041 agcttcaaga cgcccatgct gtgggcgatc ggcttcatct tcctgttcac tgtcggcggc
     2101 gtgaccggcg tggtcctgtc caacgcaggc atcgattaca gcctgcacga cacctattac
     2161 gtcgtggccc acttccacta cgtgctgtcg ctgggcgccg tcttcgcgat cttcggcggc
     2221 ttctactact ggtacgaaaa aatgttcggg gtgaagtaca acgagttcct cggctgtgcg
     2281 cacttctgga tcatgttcgt gggcgtgaac ctggtgttct tcccgcagca cttcctgggt
     2341 ctgcagggga tgccgcgccg gtatgtcgat tacgcggacg gctatgccta ctggaacatg
     2401 atctcgtcga tcggctacgc gatcacggcg gtgggcgtgc tggtcttcct ggtcatgctg
     2461 gccgagtcgg cgatccgccg gcgcaagggc gtcgccaacc cgtggggcga aggcgccacg
     2521 acgctggaat ggaccctgtc ctcgccgccg ccgcaccacc agttcaacga actgccggtg
     2581 gtaaagagcg acgaccacta gagcccggtt gatccggaac tgacacacgg ggggccgctc
     2641 tttcagtcga aagggcggcc tctgggtttt tgaaaagcaa atgaccgaaa ccacccccca
     2701 gcccgcgatc tcgatggccc agccggaaga cttcttccag ctgctgaagc cgcgcgtgat
     2761 gtcgctggtg gtgttcaccg gcgcgaccgg gctggttgtg gccaagggcg gcatcgactg
     2821 gatctcggcc gccatcgcca tcctgtgcat cgccgtgggg gccggggcgg cgggggcgct
     2881 gaacatggcg ctggagggcg agaccgacgc cctgatgcgg cgcacgcgcg gacggcccgt
     2941 ggcggcgggg cgcgtccgca agaacgacgc catggccttc ggcgtcatcc tgtcgatctt
     3001 ctcggtcatg ctgctgggca tgacgaccaa ctggctggcc ggcggcctgc tgctgctgac
     3061 catcgtctat tacgccgcct tctacaccct gctgctgaag cggcggacgc cccagaacat
     3121 cgtcatcggg ggcgccgccg gagccttccc gcccgtcatc ggctgggccg ccgcgaccgg
     3181 gacggcgccg tggcaggcct ggctcctgtt cctgatcatc ttcctgtgga ccccgccgca
     3241 cagctgggcg ctggccctgt attcggccgg cgactacgcc aaggcgggca ttccgatgat
     3301 gccggtggcg cggggggcga agagcacccg gttgcagatc ctgctctaca ccctgatctt
     3361 cgtgccggtg gcgatcgcgc cgggtctgac cggtctgggg ggtctgctgt atctggccgt
     3421 gtcgatcctc ggcggcctcg gcttcctgtt cctggccgtg cgcctgtatc gcagccgcgc
     3481 cggcgatcag cccgacaagg ccgaggccgt gggacgagag gccgcattgt atgacgtccg
     3541 cgccgaggcc aaaccggcgc gcaacctgtt cgccttctcg atcctgtacc tgatggcgct
     3601 gttcgcggcc ttgttggtcg agagcgtcgc cgccatccct ggactgtaac gaccatgacc
     3661 cgcctgaccc cggaacagct gcaagcccgc aacaagcgca acctggccat cgccggggc
//
LOCUS       DASVVN010000388         4894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_68149, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000388 DASVVN010000000
VERSION     DASVVN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68149"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VF686_06225"
     CDS             complement(<1..793)
                     /locus_tag="VF686_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661445.1"
                     /translation="MAVVSHRRIDIGGVDISYREAGPADGPVVLLPHGYPCSSFEFRN
                     LMPRLGDRWRLLAPDFPGCGYSATPDGFAYDFDGFADLLEAFLERLGVDRFALYLHDF
                     GSQIGLRLAMKAPGRIAALIIQNGDIYEDQLGPDYEPLRAYWDDPTPERKAELAKAIS
                     LDGYRHEFLNGSEAVADRIAPDLWTLHWALTDARRKAIYLEIIAGLKENRNWFPRYQA
                     WLREHRPPTLIVWGPQDGYMPEGAARAYLRDLPDAELHLVEGGHWL"
     gene            complement(856..1737)
                     /locus_tag="VF686_06230"
     CDS             complement(856..1737)
                     /locus_tag="VF686_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8661446.1"
                     /translation="MFENLTARTALPSLKALRAFEAMGRTGSATRAAAELNVTHSAIS
                     RQVKALETALGVRLFEGPKHQLRLTARGEALRTGLGAGFDALTEAVRAVRGAADVHLA
                     IHQSLAVKWLIPRLAAFERDCPGIVLHLTDLAPTATRQRGADLMVRYLDGSDLNDPGV
                     EQLAENRIGLVCTPTLAAGDLSSAPRLVARTRMGGWAEWSAVTGHAEPRGPTRTLTHL
                     HFVLDGALAGLGAAVLPWTIVAEDVVRGRLVAPLGFAPDGGALVAIRSGGETGQAARA
                     VIRWLREQAKANPPPAA"
     gene            1793..2941
                     /locus_tag="VF686_06235"
     CDS             1793..2941
                     /locus_tag="VF686_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661447.1"
                     /translation="MSLRARLIVPVLGLGQIVAFASSYYLLGVLADPMAKAMRLAPSV
                     LFTALSAAFLISAVLSPAAGRWIERSGGRRVQTWAHLAFAAALLTMALAPSAVVLIAG
                     VAALGIGMSIGLYGTAFAILVELHGAAARRSITAVSLIGALGGGLGWPVSRLLVEAWD
                     WRMACAFWALAHLAVCLPLTLAVLPKRRLAATAKAALEPIRWDRRMAQIAAIFAGGWM
                     VSTAMGAHLPRLLGQLELGAEQAAWAAGLMAASAIAARLFDLMVLHRSHPVATVRLAC
                     LFHPLGAGLAVWGGARLAPLLSVGQGAGNGLLSVASGVLPLHVFGPDRYAVRQAMILT
                     PARYLQSLAPAAYAVALDASLFLALALSSAVCLLMFALTFGLRPVSGR"
     gene            complement(2928..3725)
                     /locus_tag="VF686_06240"
     CDS             complement(2928..3725)
                     /locus_tag="VF686_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8661448.1"
                     /translation="MTLIRRERRAHIEIWTLDRPDRLNALPDLQDGETFAAACEAVNA
                     DPDVRCVVLTGAGRAFSAGGDLKAMKDRRDMFAGSGADIRERYRRVVHRIVRSLYGLE
                     VPLVAAVNGPAMGLGCDIAGLADIRIASETASFGVPFLKLGILPGDGGAWLMPRNVGY
                     ARAAEMLFTARVLDARTAMEWGLVSRVVAPDRLMDEALEIAEEIAARAPQALRMTKAL
                     LRQGRDATFDQILEMTASMQALAHLGDDHLEGVTAALEKRPADFSGR"
     gene            complement(3763..4089)
                     /locus_tag="VF686_06245"
     CDS             complement(3763..4089)
                     /locus_tag="VF686_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661449.1"
                     /translation="MLKLEATLVATMAARSRRSGWSPDGGVDFVLWAPKPQASIVAPV
                     EARRTVADEPASFLDADEILTPGVAVVRRYVSLAAQVAEVAAREPVERPLTRAWSSVA
                     RKVPGS"
     gene            complement(4220..4375)
                     /locus_tag="VF686_06250"
     CDS             complement(4220..4375)
                     /locus_tag="VF686_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661450.1"
                     /translation="MNDRDRNLQHAMMSFALWGGMLVNTKNTNGAGDRVVSHPSNFVP
                     IKIEKRR"
     gene            4531..4761
                     /locus_tag="VF686_06255"
     CDS             4531..4761
                     /locus_tag="VF686_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX8661451.1"
                     /translation="MSSTVAQNPQAPPRAIATPCIKVCVVDGASSLCLGCFRTLSEIG
                     GWSGLTDEQRAAVMADLPARRSRVDPALLGSA"
BASE COUNT          703 a         1793 c         1577 g          821 t
ORIGIN      
        1 gcagccaatg gccaccttcg accaaatgca gctccgcgtc gggaagatcg cgcagatagg
       61 cccgagccgc cccttccggc atgtatccgt cctgcggtcc ccagacgatc agcgtcggcg
      121 gccggtgctc gcgcagccag gcctgatagc gggggaacca gttccggttc tccttcaggc
      181 cagcgatgat ctcgaggtaa atcgccttgc gccgggcgtc agtcagcgcc cagtgcaggg
      241 tccatagatc gggcgcgatc cggtcggcga ccgcttcgct gccgttcagg aattcgtggc
      301 gatagccgtc caggctgatg gcctttgcca gctccgcctt gcgctcgggc gtggggtcgt
      361 cccagtaggc cctgagcggc tcatagtccg gccccagttg atcttcgtag atgtcgccgt
      421 tctggatgat cagcgcggcg atgcggcccg gcgccttcat cgccagccgc aagccgatct
      481 gcgagccgaa atcatggagg tagagcgcga accggtcgac gccaagccgc tccagaaacg
      541 cctccagcag gtcggcgaac ccgtcgaagt cataggcgaa gccgtccggc gtcgcgctgt
      601 agccgcaccc cggaaaatcg ggcgccagca accgccagcg gtcgccgagc cgcggcatca
      661 ggttgcggaa ctcgaaagac gagcagggat agccatgcgg cagcagaacc acgggcccgt
      721 ccgcgggacc ggcctcgcgg taggagatat cgacgccgcc gatgtcgatg cggcggtggg
      781 atacgacggc catccggacc tccgggcgcg gggacgtcct gtgcaacgcc ctggccgggc
      841 cgcgggtttc ggtcgtcagg ccgcgggcgg cggatttgcc ttcgcctgtt cccgcagcca
      901 gcggatcacg gcccgtgccg cctgtccggt ttccccaccg ctccggatcg cgaccagcgc
      961 cccgccatcc ggggcgaaac cgagcggcgc gaccagccgt ccccgcacga catcctcggc
     1021 cacgatcgtc cacggcagga cagccgctcc caggcctgcc aaggccccgt ccagcacgaa
     1081 atgcaggtgg gtcagggtgc gcgtcggacc ccgaggctcg gcgtggcccg tcacagccga
     1141 ccactccgcc cagccgccca tccgcgtgcg cgccaccaga cggggggccg aggacaggtc
     1201 cccggccgcg agcgtcggcg tgcagaccag gccgatgcgg ttctccgcca gctgttcgac
     1261 tcccggatcg ttcaggtcgg agccatccag atagcggacc atcagatcgg cgccccgctg
     1321 ccgggtggcc gtcggcgcca gatccgtcag atgcagcacg atgccgggac aatcccgctc
     1381 gaaggcggcc agccgcggga tcagccattt gaccgccaga ctctggtgga tcgccagatg
     1441 aacatccgcc gcgccgcgca ccgcgcgcac cgcctcggtc agggcgtcga acccggcgcc
     1501 cagtcccgtc cgcagcgcct cgccgcgggc agtgagccgc agctgatgtt tcggtccctc
     1561 gaacagccgg acgcccagcg ccgtctccag cgccttcacc tgccggctga tcgcgctgtg
     1621 ggtgacgttc aattcggccg ccgcgcgcgt ggccgaaccg gtccgtccca tcgcctcgaa
     1681 cgcccgcaag gccttgaggg agggcagcgc cgttcgagct gtgagatttt cgaacataca
     1741 gcgttcgata tgtcgctttc cgcgcgggcg caatgcggcc atccggggac ccatgtccct
     1801 gcgcgcccgt ctcatcgttc ccgttcttgg tctgggacag atcgtggcct tcgcctccag
     1861 ctactatctg ctgggtgtcc tcgccgaccc gatggcgaag gcgatgcggc tggcgccctc
     1921 cgtcctgttc acggccctgt ccgccgcctt cctgatctcg gcggtgcttt cgccagcggc
     1981 ggggcgctgg atcgaacgca gcggcgggcg gcgcgtacag acctgggcgc acctcgcctt
     2041 cgccgccgcc ctgctgacca tggccctggc gccgtccgcc gtggtcctga tcgcgggcgt
     2101 tgcggccttg gggatcggca tgtcgatcgg cctctacggc acggccttcg ccattctggt
     2161 ggaactgcac ggcgcggccg cccgacgctc gatcaccgcc gtttcgctga tcggcgccct
     2221 gggtggcgga ttgggctggc cggtcagccg cctgctcgtc gaggcatggg actggcgcat
     2281 ggcctgcgcc ttctgggcgc tggctcatct ggcggtctgc ctgccgctca cgctggcggt
     2341 cctgccgaaa cggcgcctcg cagccacggc gaaggccgcg ctcgagccga tccggtggga
     2401 ccggcgcatg gcgcagatcg ccgccatatt cgccggagga tggatggtct ccaccgccat
     2461 gggcgcacac ctgccccgcc tgctcggcca gcttgagctc ggcgccgaac aagctgcgtg
     2521 ggccgcaggc ctgatggcgg cgagcgccat cgccgcacgt ctgttcgacc tgatggtcct
     2581 gcatcggtcg catcccgtgg ccacagtgcg gctggcctgc ctgttccatc cgctcggcgc
     2641 cggactggct gtctggggcg gcgcacggct ggctccgttg ctctccgtgg ggcagggcgc
     2701 gggtaacggc ctgttgtcgg tggcctctgg cgtgctgccg ctgcacgtct ttggtcccga
     2761 ccggtacgcc gtgcgccagg ccatgatcct gacgcccgcg cgctatctcc agtcactggc
     2821 gcccgccgcc tatgctgtgg ctctggacgc ctcgcttttt ctggcgctcg cgctttccag
     2881 cgccgtctgc ctgctgatgt ttgctctgac cttcggcctg cgccccgtca gcggccgctg
     2941 aaatccgccg gccgcttctc cagcgccgcc gtcacgcctt ccagatggtc gtcgcccaga
     3001 tgcgccagcg cctgcatcga cgccgtcatc tccaggatct ggtcgaaggt cgcgtcgcgg
     3061 ccctgacgca gcagggcctt ggtcatgcgc agggcctggg gcgcgcgggc ggcgatctcc
     3121 tccgcgatct ccagcgcctc gtccatcagc cggtcgggcg cgaccacacg gctgacgaga
     3181 ccccactcca tcgccgtccg cgcgtccagc acccgggcgg tgaacagcat ctcggccgcc
     3241 cgcgcatagc cgacgttgcg cggcatgagc caggccccgc catcgcccgg caatatgccc
     3301 agcttcagaa agggcacgcc gaagctggcc gtctccgagg cgatgcggat gtcggccagc
     3361 ccggcgatgt cgcagcccag ccccatggcc gggccgttca ccgccgccac cagcggaacc
     3421 tccagcccgt acagcgaccg cacgatccgg tgcaccacgc ggcgatagcg ctcgcggata
     3481 tccgcgcccg aaccggcgaa catgtcgcgc cgatccttca tcgccttcag atcgcccccc
     3541 gccgaaaacg ctcgtccggc gccggtcagc accacgcacc gcacgtccgg atccgcgttc
     3601 accgcctcgc aggccgcggc gaaggtttcg ccgtcctgca gatcgggcag ggcgttcaac
     3661 cggtcgggcc ggtccagcgt ccagatttcg atgtgtgcgc ggcgctcgcg gcggatcagg
     3721 gtcatatgtc cgctgtgccg caagcgcggc cccgccgcaa cgtcaggagc cggggacctt
     3781 ccgcgccacg gacgaccacg cccgcgtcag gggacgttcg accggctcgc gggccgctac
     3841 ctcggccacc tgcgcggcaa gactgacgta tcgccgaacg accgccactc cgggagtcag
     3901 aatctcatcg gcgtcgagga agcttgccgg ttcgtcggcc acggtccgcc tcgcctcgac
     3961 cggcgccacg atgctcgcct gcggtttcgg agcccagagg acgaagtcga ccccgccgtc
     4021 cggcgaccag ccgcttcgcc tcgaccgcgc ggccattgtc gcgaccagcg tcgcctcaag
     4081 cttcagcata tacgcgcccc tccggcagaa gactggcacg gatttcatcc cgacgcctgt
     4141 atcccgcgcg cgacagaaga attgaaaaag ccccggacct tgcgggccgg ggcttttgtc
     4201 agggttcgag cccagcgccc tagcggcgct tctcgatctt tatcggcacg aagttcgacg
     4261 ggtggctgac aacacggtcg cccgccccgt ttgtattctt cgtattcacc agcatcccgc
     4321 cccagagggc gaatgacatc atggcgtgct ggaggttgcg gtctctgtcg ttcatccttg
     4381 gcgtctcctt tcgatggaac gatgaaaaga gacgcgtcgt gcttttcggg aatctcaccg
     4441 tcatagtcgt ttcgcgtcag catccgtgta acctcggctg ctgaaaattg caacgatttt
     4501 tactccgcta gcggaccata ttttgcactg atgtcatcga ctgtcgccca aaacccacag
     4561 gctccgccgc gggctatcgc cacgccgtgc atcaaggtgt gcgtcgtcga cggcgcctcg
     4621 agtctgtgtc tgggctgctt ccgcaccctg tccgagatcg gcggctggag cggtctgacc
     4681 gacgaacagc gcgccgccgt gatggccgat cttccggccc ggcggtcccg cgtcgatccg
     4741 gcgctgctgg gctccgcctg actgccccgt cacgtccgcc gttaagcctg cgcaaaccat
     4801 cccccttcac cgcatccaaa ctcgcctgtg ggataccgga gccgtcgcag aagcggcgct
     4861 tcaggacgag taacgcatgc gtttcgacct ttcc
//
LOCUS       DASVVN010000389         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_462349,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000389 DASVVN010000000
VERSION     DASVVN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462349"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..888)
                     /locus_tag="VF686_06260"
     CDS             complement(142..888)
                     /locus_tag="VF686_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX8661452.1"
                     /translation="MIRTLLIITGASLVLCIATLSGAAAIGGNDLARQGWTWTFRDDG
                     EDHTVHFERGDDSPAVTRNLAWTGGDTLAVEIPGDVTYVQGDTPSVVVTGKKSMVDRV
                     RLVDGRLTWADGDADHVVFGWHNNSRLRVVVTAPSVKRFDLQSSADLSIRGYDQPTFD
                     LNVSGSGDVDASGETQTATIDTTGSGDSDLSALELTDADIDTSGSGDLRVGPTGTARI
                     DISGSGDVDLTRRPANLTQSVSGSGDIDQN"
     gene            complement(902..1486)
                     /locus_tag="VF686_06265"
     CDS             complement(902..1486)
                     /locus_tag="VF686_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEX8661453.1"
                     /translation="MTRAEFMGRLRRGLVGMPTAAAADIASDYETHFEDGAAAGRSEA
                     EVAAALGDPDRLARELRAEAGAQRWHQEKNPSAAAGAVFAVLGLGAIDLLILLPIFMS
                     VVGVLFGFFMAAVGLFIGGGVVMIAGPVAGFPGGVATAILGGIGLMAGATALAALTAI
                     VTILLVNATVWYARLHYRLLKPALGEQYPADGKF"
     gene            complement(1483..1854)
                     /locus_tag="VF686_06270"
     CDS             complement(1483..1854)
                     /locus_tag="VF686_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8661454.1"
                     /translation="MPETIEIQLKKGVLTLCVLALLNRHDSYAYEIAATLSDAIDMGE
                     GTIYPLMRRMQSDGQVETYMVESASGPSRKYYRLTDAGRSALEAQTAEWRAFARAVES
                     VLDGAAPAAAPAKKADKGAEL"
     gene            2108..>2427
                     /locus_tag="VF686_06275"
     CDS             2108..>2427
                     /locus_tag="VF686_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEX8661455.1"
                     /translation="MGMGTRARASIGGGFTALCLAVFSATPTWAKELVGQPTDGALGL
                     QPAASPLKVQAHFFHDIILMPIITFITLLVLGLILWICVRYNKKANPVPAKWSHNTLV
                     EVIWT"
BASE COUNT          391 a          782 c          839 g          415 t
ORIGIN      
        1 gtcgtcggcc ccttcgaggg cgccttgcag aatggagagg gcttcgtcgg cgccgacgtc
       61 ggcgctgtcg agaatggcgg gaggcgatac gaggacggtc atgagcccta agtaggggct
      121 gaccgccctc gcgtcaccgg gtcagttctg gtcgatgtcg cccgagcccg acacggactg
      181 ggtaagattg gccggccggc gggtcagatc gacgtcgccc gagcccgaga tgtcgatccg
      241 ggccgtgccg gtcgggccga cccgaaggtc gcccgaaccg gaggtgtcga tgtcggcgtc
      301 ggtcaactcc agcgccgaca ggtcggagtc gccggaccct gtggtgtcga tcgtggcggt
      361 ctgtgtctcg ccggaggcgt ctacgtctcc cgaacccgaa acgttgagat cgaaggtcgg
      421 ctggtcgtat ccgcggatcg acaggtcggc cgagctctgc agatcgaagc gcttcacgga
      481 cggcgcggtg accaccaccc gaaggcggct gttgttatgc cagccgaaga ccacgtgatc
      541 ggcgtcgccg tcggcccagg tcaggcggcc gtcgaccaga cggaccctgt cgaccatcga
      601 cttcttgccc gtgaccacaa ccgagggcgt gtcgccctgg acataggtca cgtcgcccgg
      661 gatttcgacg gccagggtgt cgccgccggt ccaggcgagg ttgcgggtga cggccgggct
      721 gtcatcgccc cgctcgaagt ggaccgtgtg gtcctcgccg tcgtcgcgga aggtccacgt
      781 ccagccttgg cgggccaggt cgttgccgcc gatggcggcc gctccggaca gggtggcgat
      841 gcagagcacg aggctggcgc cggtgatgat gagaagcgtg cggatcatgt tcgttcctcc
      901 ctcagaactt gccgtcggcc gggtattgtt ccccgagcgc aggcttcagc aggcgatagt
      961 gcaggcgcgc gtaccagacg gtcgcgttga ccagcaggat ggtgacgatg gcggtcaggg
     1021 cggccagggc ggtggctccg gccatcaggc cgataccgcc caggatcgcc gtggccacgc
     1081 ctccggggaa gcccgcgacg ggcccggcga tcatcaccac gcccccgccg atgaacagtc
     1141 cgaccgcggc catgaagaag ccgaacagga cgccgaccac gctcatgaag atcggcagca
     1201 ggatcagcag gtcgatcgcg cccagtccca gcaccgcgaa cacggctccg gcggcggccg
     1261 acgggttctt ctcctgatgc cagcgctggg cgccggcctc ggcgcgcagt tcgcgggcga
     1321 gacggtcggg atcgcccagg gcggccgcga cctcggcttc ggagcggccg gcggcggctc
     1381 cgtcctcgaa atgggtctca tagtccgagg cgatgtcggc ggcggcggcg gtcggcatgc
     1441 cgaccagtcc gcggcgcaaa cggcccatga attcggcgcg ggtcatagtt cagctccctt
     1501 gtcggctttt ttcgcaggcg ctgcggcagg cgccgcgccg tcgagaacgg attccaccgc
     1561 gcgggcgaag gcgcgccatt cggccgtctg ggcctccagg gcgctgcggc cggcgtcggt
     1621 cagacgatag tatttgcgtg acggcccgga cgccgattcg accatatagg tctcgacctg
     1681 tccgtcggac tgcatccgcc gcatcagcgg gtagatcgtc ccttcgccca tgtcgatcgc
     1741 gtcggacagg gtcgcggcga tctcataggc atagctgtca tgccggttca gaagcgccag
     1801 aacgcatagc gtcagcaccc ccttctttag ctgtatctcg attgtttcgg gcacgtggcg
     1861 tcctccttcg atgcccaaga cgtatcgcaa ggtatctggt gacacaaggt agctgtcata
     1921 acatgacgag gaattaatcc gggggccgat ggccgcagtc gcgcgcaatc tcttgctcat
     1981 cgcttggcaa gcgtcagacg gggcgatatg gaccgcccga agaaacgctg cgggcgtcag
     2041 tccgggcgcc tgcttgcgat tgtctcgcaa gcgtttgcga cgaaacggta atatgaggat
     2101 gtctcgcatg gggatgggca cgcgggcccg ggcgtcgatc ggcggtggtt tcaccgccct
     2161 gtgcctggcc gtattctccg ccacgccgac gtgggcgaag gaactggtgg gtcagccgac
     2221 cgatggagcc ctcgggctgc agccggccgc ctcgccgctg aaggttcagg cgcacttctt
     2281 ccacgacatc atcctgatgc cgatcatcac cttcatcacc ctgctggtgc tgggcctgat
     2341 cctgtggatc tgcgtccgct acaacaagaa ggccaaccct gttccggcca agtggagcca
     2401 caacaccctg gtcgaggtga tctggac
//
LOCUS       DASVVN010000390         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_462366,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000390 DASVVN010000000
VERSION     DASVVN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462366"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1257)
                     /locus_tag="VF686_06280"
     CDS             complement(103..1257)
                     /locus_tag="VF686_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8661456.1"
                     /translation="MKVKLLASVATAGLLAAGAAAAEPNGWYGAIDAGYHTLGDVETR
                     SQTTSALFDIGTEGDWAAFARLGYRFDQNWRVELEGGYRQNDIEMIDRPGPGGVPSGV
                     CNISTGACVTPDGELKAATLMMNVLFDFGSEGDALRPFIGLGAGVNRVDTGFIGTFQN
                     ARTVGVGADDSSTKLAAQGIAGLAWAISDRAHLDLTYRYLVSNMEFNTFTSTAASAIG
                     PFQGRYDESHTVTLGLRYAFGADEPAYVPPPPPPPPPPPPPPPPPPPPPVATVQPARE
                     FVVYFDWDRSDLTAEASSVVTQAANYAKSGRPTRVLVVGHADTSGSAAYNVGLSNRRA
                     RTVADALVAQGVNGGVISLDGKGETALARPTADGVREPLNRRATIGINFR"
     gene            1454..>2255
                     /locus_tag="VF686_06285"
     CDS             1454..>2255
                     /locus_tag="VF686_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661457.1"
                     /translation="MAIGFEGRVAIVTGAGGGLGREHALALAKRGAKVVVNDLGGARD
                     GSGGSATATESVVAEIRALGGEAIANAASVTDFAAVEAMVRAAMAEWGRVDILVNNAG
                     VLRDKTFAKMDLDDFRFVMDVHLMGAVNCTKAVWDIMRERNYGRIVMTSSSSGLYGNF
                     GQSNYGAAKMALVGLMQTLSIEGAKNDIRVNCLAPTAHTRMTEDLGARLPLELLGPET
                     VSPGLLYLVSEEAPSRCILMAGAGGFERAYVTLTQGIRAVGDDAPEQVA"
BASE COUNT          360 a          721 c          815 g          359 t
ORIGIN      
        1 agcaaaaacc gccggccctc tttcgaggac cggcggctga tgttgtcttg cctggcgtcc
       61 ttcggcttga gggccgaagg ggggccgatc ggccttccgg ggttagcgga agttgatgcc
      121 gatagtggcg cgacggttga gcggctcgcg cacgccgtcg gcggtcgggc gggccagggc
      181 ggtttcgccc ttgccgtcca gcgagatcac gccgccgttg acgccttgag caaccagggc
      241 gtccgcaacg gtgcgggcgc ggcggttgga caggccgacg ttataggcgg ccgagcccga
      301 ggtgtcggcg tggccgacga ccagcacgcg ggtcggacgg cccgacttgg cgtagttggc
      361 cgcttgcgtg acgaccgagg aggcttcggc cgtgaggtcc gaacgatccc agtcgaagta
      421 gaccacgaac tcacgcgccg gctgaaccgt cgccaccgga ggcggcggcg ggggaggcgg
      481 cggcgggggc ggcgggggcg gaggcggcgg gggcggcggc acataggccg gctcgtccgc
      541 gccgaacgca tagcgcaggc ccagggtcac ggtgtggctc tcgtcgtaac ggccctggaa
      601 gggaccgatg gccgaggccg ccgtcgaggt gaaggtgttg aactccatgt tcgacaccag
      661 gtagcgatag gtcaggtcca gatgggcccg gtcgctgatc gcccaagcca ggccggcgat
      721 gccttgcgcg gccagtttgg tcgagctgtc gtcagcgccg acgccgacgg tgcgggcgtt
      781 ctggaaggtg ccgatgaagc cggtgtcgac gcggttcacg ccggcgccca gaccgatgaa
      841 cggacgcagg gcgtcgccct cgctgccgaa gtcgaacagc acgttcatca tcagggtggc
      901 ggccttgagc tcgccgtccg gagtcacgca ggcgccggtc gagatgttgc acacgccgct
      961 cggaacgccg cccggacccg gacgatcgat catctcgatg tcgttctggc ggtagccgcc
     1021 ttccagctcc acgcgccagt tctggtcgaa ccggtaaccc agacgcgcaa acgcggccca
     1081 gtcgccttcg gtgccgatgt caaacagagc gctcgtcgtc tgcgaccgcg tctctacgtc
     1141 gcccaacgtg tggtagcccg cgtcaatcgc gccataccag ccgttcggct cagccgccgc
     1201 cgcgccggct gcaagcagcc ccgccgtcgc gacgctggcg agaagtttca ccttcatcat
     1261 atccctcatg ctcgttgttt gcccgaagtc ccaatgtata cgggacgctc acggtgccga
     1321 acgacaatgt acggtcgtgg ttcccgattt tggctacgaa tgcggcgact gtggcgccgt
     1381 ttcaacacat tgacgcaggc tttcccgaaa cgagacgcct gccaggcagt tatccgggca
     1441 cgaggagcgc gatatggcga ttggatttga gggccgggtg gccattgtca cgggagccgg
     1501 aggcgggctg gggcgcgagc atgccctcgc cctggcgaaa cgcggcgcaa aagtggtggt
     1561 caacgacctg ggcggcgcgc gcgacgggtc gggcggctcg gcgacggcga ccgaatcggt
     1621 cgtggccgag atccgggcgc ttggcggcga ggcgatcgcc aacgcggcct cggtcaccga
     1681 cttcgccgcc gtcgaggcga tggtccgggc ggcgatggcc gaatggggcc gggtcgacat
     1741 cctggtcaac aacgccggcg tcctgcgcga caagaccttc gccaagatgg atctggacga
     1801 tttccgcttc gtcatggacg tgcacctgat gggcgcggtc aactgcacca aggcggtgtg
     1861 ggacatcatg cgcgagcgga actacggccg catcgtcatg acctcctcct cctcgggcct
     1921 gtacgggaat ttcggccagt ccaactatgg tgcggccaag atggcgctgg tcggcctgat
     1981 gcagaccctg tcgatcgagg gggcgaagaa cgacatccgc gtcaactgtc tggccccgac
     2041 cgcccatacg cgcatgaccg aggatctggg cgcccgcctg ccgctggagc tgctggggcc
     2101 cgaaacggtg tctccgggcc tgctgtatct ggtcagcgag gaggcgccga gccgctgcat
     2161 actgatggcc ggggccggcg gtttcgaacg cgcctatgtg accctgacac agggcatccg
     2221 ggcggtcggc gacgacgccc ccgaacaggt ggccg
//
LOCUS       DASVVN010000391         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_225699,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000391 DASVVN010000000
VERSION     DASVVN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225699"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF686_06290"
     CDS             complement(<1..251)
                     /locus_tag="VF686_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX8661458.1"
                     /translation="MTLQTNIRAMKKTLVLIAAAAGLAFAANPAAAEGGAHALRDGGF
                     AFDGPFGAFHQGQLQRGYKVYREVCASCHSMNLMHFRTLA"
     gene            complement(260..1591)
                     /locus_tag="VF686_06295"
     CDS             complement(260..1591)
                     /locus_tag="VF686_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX8661459.1"
                     /translation="MSDHESTYVPKTGIEKWFDSRLPIIRFGADYMSLPTPKNLNYWW
                     TFGGILALCLVVQIATGVILAMHYVPNTELAFASVERIMRDVNGGDLIRYTHANGASM
                     FFVAVYLHMLRGLYYGSYKAPREMIWIVGCLIFFLMIATAFLGYVLPWGQMSYWGAEV
                     ITNLIGAIPLVGEPILIWLRGGPAIDNATLNRFFSLHYLLPFVIAGCVVLHLWALHHA
                     GQNNPVGILIPKDRMAKDTVPFHPYFTVKDGFAIVLFLILFAVFVFFMPNALGHADNY
                     IPANPLVTPAHIVPEWYMLPFYAILRAVPDKFGGVVAMFGAIGVLFVLPWLDTSKVRS
                     MRYRPAMRTFFVIFVLVGLILGWCGAQLPDAPVIGDFKTFVLIDGDLNSYLWLTRLAT
                     LYYFAFFLVIMPLVGLRETPRPIPATISEPVLPGGGDAMTAAAPASAEKKG"
     gene            complement(1603..2145)
                     /gene="petA"
                     /locus_tag="VF686_06300"
     CDS             complement(1603..2145)
                     /gene="petA"
                     /locus_tag="VF686_06300"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX8661460.1"
                     /translation="MAESVVTENGTGGDASRRDFIHIAAGAAAVGAGAMLAWPLINSM
                     NPAADTLALASIEFDMSKVQEGQQVVIKWQGKPVFVRYRTPREIQEAVATPLSALKDP
                     QTDADRIKAGHQQYLVLLGNCTHLGCIPTFGAGDFGGWFCPCHGSHYDTSGRIRKGPA
                     PKNLVVPEYDYVSDTRIKIG"
     gene            2342..2794
                     /locus_tag="VF686_06305"
     CDS             2342..2794
                     /locus_tag="VF686_06305"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX8661461.1"
                     /translation="MRLALFQPAIPQNVGACIRLGACFGVELHVIEPTGFRFDDRAMK
                     RAALDYGPLGHMRRHADWDAFQRDRGQNTPMGRLVLFTTKGATPLNDFVFRPDDTLLF
                     GSEPSGAPDFVHEAADARVVIPIQPEARSLNLSVSAGIALWEGLRQTL"
     gene            complement(2796..>3092)
                     /locus_tag="VF686_06310"
     CDS             complement(2796..>3092)
                     /locus_tag="VF686_06310"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8661462.1"
                     /translation="IADVVAHIQHVRDTAGIDHVGLGGDFDGVDALPVGLEGVDGYPR
                     LLAALMANGWTEADIRKLAGENALRVMRAVEAVAESRKGERPNLAVIPAEGTPE"
BASE COUNT          546 a          996 c         1059 g          491 t
ORIGIN      
        1 gccagggtgc ggaaatgcat gaggttcatg ctgtgacacg aggcgcagac ctcgcgatac
       61 accttgtagc cgcgttgcag ctggccctga tggaaggcgc cgaacgggcc gtcgaaggcg
      121 aagccgccgt cacgcagcgc atgggcgccg ccctcggccg cggccgggtt ggctgcgaac
      181 gccaggccgg cggcggctgc gatcaggacc agggtcttct tcatggcgcg gatattcgtc
      241 tggagggtca tggtcgcgat cagcccttct tctcggccga ggccggagcc gccgccgtca
      301 tggcgtcgcc gccgccgggt aggaccggtt ccgagatcgt cgccgggatg ggccgcggcg
      361 tctcgcgcag gccgaccagc ggcatgatga cgaggaagaa ggcgaagtag tagagcgtcg
      421 ccagccgggt cagccacagg tagctgttca gatcgccgtc gatcaggacg aaggtcttga
      481 agtcgccgat caccggcgcg tccggcagct gcgcaccgca ccagcccagg atcaggccga
      541 ccagaacgaa gatgacgaag aaggtccgca tcgccgggcg atagcgcatc gaccgcacct
      601 tggacgtatc cagccacggc aggacgaaca gcacgccgat ggcgccgaac atcgccacca
      661 cgccgccgaa cttgtccgga acggcccgca ggatcgcgta gaagggcagc atgtaccact
      721 cgggcacgat gtgcgccggg gtcaccagcg ggttggccgg aatgtagttg tcggcgtggc
      781 ccagggcgtt cggcatgaag aacacgaaca cggcgaacag gatcaggaac aggacgatgg
      841 cgaagccgtc cttgaccgtg aaatacgggt ggaagggcac ggtgtccttg gccatccggt
      901 ccttcgggat caggatcccg accgggttgt tctgaccggc gtggtgcagg gcccaaaggt
      961 gcagcaccac gcagccggcg atcacgaacg gcagcagata gtgcagcgag aagaagcggt
     1021 tcagggtggc gttgtcgatc gccgggccgc cacgcagcca gatcaggatc ggttcgccga
     1081 ccagcgggat tgccccgatc agattggtga taacctcggc gccccagtag gacatctggc
     1141 cccacggcag gacgtagccg aggaaggcgg tggcgatcat caggaagaag atcaggcagc
     1201 cgacgatcca gatcatctcg cgcggcgctt tatacgagcc gtagtacagc ccgcgcagca
     1261 tgtgcagata gaccgcgacg aagaacatcg aggcgccgtt ggcgtgggtg tagcggatca
     1321 ggtcgccgcc gttgacgtcg cgcatgatgc gttcgaccga ggcgaaggcc agttcggtgt
     1381 tgggcacata gtgcatggcc aggatcacgc cggtcgcgat ctgcacgacc aggcagagag
     1441 ccaggatgcc gccgaaggtc caccagtagt tgaggttctt cggggtcggc agcgacatgt
     1501 agtcggcgcc gaagcggatg atcggcaggc ggctgtcaaa ccacttttca atgccggtct
     1561 tggggacgta ggtggattcg tgatcgctca tcggtctcgc cctcagccga tcttgatgcg
     1621 ggtgtcggag acgtaatcgt actccggcac gacgaggttc ttgggcgcgg gacccttacg
     1681 gatgcggccc gaggtgtcgt agtgcgaacc gtggcagggg cagaaccagc cgccgaaatc
     1741 accggcgccg aacgtcggga tgcagcccag gtgggtgcag ttgccgagca gcaccagata
     1801 ctgctgatga ccggccttga tgcggtcggc gtcggtctgc gggtccttca gggccgacag
     1861 cggggtcgcc acggcttcct ggatttcccg cggcgtgcgg tagcggacga agacgggctt
     1921 gccctgccac ttgatgacga cctgctggcc ttcctggacc ttgctcatgt cgaactcgat
     1981 cgacgccagc gccagggtgt cggcggcggg gttcatcgaa ttgatcagcg gccaggccag
     2041 catggccccg gcgccgacgg cggccgcgcc ggccgcgata tggatgaagt cccggcggga
     2101 agcgtcgccg cccgttccgt tctcagtcac gaccgattcg gccacgtctt actcctcgtg
     2161 cgccgcgatc cgcgcccgac agggggtgcg gcgaaaaacg gcatggatgc tttagcggct
     2221 tcccgtcggt ctggcccggc ccggtgttgc ggctcatcgc cgcaaactgc gccgctcctt
     2281 gagaaacagt cgcaattcga gccgcgacag acccccaggc cgcgcgtatg agggccttag
     2341 aatgcgtctc gccctttttc aaccagccat tccgcagaac gtgggcgcat gcatccgcct
     2401 gggcgcctgt ttcggcgtgg aactgcacgt catcgagccg accggcttcc gcttcgacga
     2461 ccgcgcgatg aagcgcgcgg cgctggacta cggccccttg ggccacatga ggcggcacgc
     2521 ggactgggat gccttccagc gcgaccgcgg ccaaaacacg cccatgggcc ggctggtcct
     2581 gttcacgacg aagggcgcga cgcccctgaa cgatttcgtc ttccggcccg acgacaccct
     2641 gctattcggc agcgaacctt cgggcgcccc ggacttcgtc cacgaagccg cggacgcgcg
     2701 ggtggtgatc ccgatccaac cggaagcacg ctcactgaac ctgtcggtct cggccgggat
     2761 cgcgctgtgg gaagggctgc ggcagactct ctgacctatt ccggcgtgcc ctcggccggg
     2821 atcacggcca gattcggccg ctcgcccttc cggctctccg ccaccgcctc gaccgcgcgc
     2881 atgacccgca gcgcgttctc gccggccagc ttgcggatat ccgcctcggt ccagccgttg
     2941 gccatcaggg cggccagcag acgcggatag ccgtcgacgc cctccagccc gaccggcagg
     3001 gcgtcgaccc cgtcgaagtc gccgcccaga ccgacgtgat cgatccctgc tgtgtcgcgc
     3061 acatgctgga tgtgggcgac cacgtcggcg at
//
LOCUS       DASVVN010000392         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_147319,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000392 DASVVN010000000
VERSION     DASVVN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147319"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /gene="dnaX"
                     /locus_tag="VF686_06315"
     CDS             <1..1492
                     /gene="dnaX"
                     /locus_tag="VF686_06315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8661463.1"
                     /translation="ALNTETDTIDRPSLALTDQGRHDASIKAGQHMDVVEMDAASHTG
                     IDGIRDIIESVRYAPVEARYKVYVLDEVHMLSNQAFNALLKTLEEPPPHAKFIFATTE
                     IRKVPVTILSRCQRFDLRRVEPEILVEHLARIADREGMKIEQDALALIARAAEGSVRD
                     GLSLLDQALVQGERGETVKTEIVRDMLGLADRSQTIALFESIMAGRTPEALELFRTLY
                     GYGADPVQVTNDLLEHCHAASVAKMLGPQATRLPNDQAQKLTALGAVISAGTLARVWQ
                     MLLKALDEVRRAPNPADAVEMAIVRLAYAADLPGPEEALKALRDGEPMPGGAAPRGPS
                     GGGGGGGAQASLGAQLAARPVAAPSLPDPQSFEAVVALIGEKREVGLQMDVQRYVRPV
                     AFKPGAITYESLDGAPIDLARKLASRLKDWTGRTWLIAANGQGGGETMIEIDRRKRSE
                     ERAQIEADPFVMAVMEAFPGAKIGDIKTLAPAVELPAIPDEAMDED"
     gene            1621..1860
                     /locus_tag="VF686_06320"
     CDS             1621..1860
                     /locus_tag="VF686_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661464.1"
                     /translation="MKKIVLASMLAASAIVTMPTAALADTDDACMLRNGCVWASGNDS
                     NPGYWQCRSPAVYARCVSPEGGPEDPSLTFKPIKD"
BASE COUNT          323 a          676 c          624 g          262 t
ORIGIN      
        1 cgccctgaac accgaaaccg atacgatcga ccgcccgtcg ctggccctga ccgatcaggg
       61 ccgccacgac gcctccatca aggccggcca gcatatggac gtggtcgaga tggacgccgc
      121 ctcccacacc ggcatcgacg gcatccgcga catcatcgag agcgtccgct acgccccggt
      181 cgaggcgcgc tacaaggtct acgtcctcga cgaagtccac atgctgtcca accaggcctt
      241 caacgccctg ctcaagacgc tggaagagcc gccgccccac gccaaattca tcttcgccac
      301 caccgagatc cgcaaggtgc cggtgaccat cctgtcccgc tgccagcgtt tcgacctgcg
      361 ccgcgtcgag ccggaaatcc tcgtcgaaca tctcgcccgc atcgccgacc gcgaggggat
      421 gaagatcgag caggacgctc tggccctgat cgcccgcgcc gccgagggct cggtccgcga
      481 cggcctcagc ctgctggatc aggccctcgt ccagggcgag cgcggcgaga cggtgaagac
      541 cgagatcgtg cgcgacatgc tcggcctcgc cgaccgcagc cagaccatcg ccctgttcga
      601 atcgatcatg gctggccgca cgcccgaggc gctggagctg ttccgcaccc tctacggcta
      661 cggcgccgat ccggtgcagg tgaccaacga cctgctggaa cactgccacg ccgcctcggt
      721 ggccaagatg ctgggcccgc aggcgacgcg cctgccaaac gatcaggctc agaagctgac
      781 ggcgctgggc gcggtcatct cggccggcac cctggcccgt gtctggcaga tgctgctcaa
      841 ggcgctggac gaggtccgcc gcgcgcccaa tcccgccgac gccgtggaga tggccatcgt
      901 ccgcctcgcc tacgccgccg acctgccggg tccggaggag gcgctgaagg ccctgcgcga
      961 cggcgagccc atgccgggcg gcgcggcccc tcgcggtcct tccggcggag gcggaggcgg
     1021 cggcgcgcag gcgagccttg gggcccagtt ggccgcccgc ccggtcgccg cgcccagcct
     1081 gcccgacccg cagtcgttcg aggctgtcgt ggccctgatc ggcgagaagc gcgaggtcgg
     1141 cctgcagatg gacgtccagc gctacgtccg ccccgtggcc ttcaagcccg gcgccatcac
     1201 ctatgaaagc ctcgacggcg ccccgatcga tctggcccgc aaactggcgt cccggctcaa
     1261 ggactggacc ggtcgcacct ggctgatcgc cgccaacggc cagggcggcg gcgagaccat
     1321 gatcgagatc gaccgccgca aacgctccga ggagcgggcg cagatcgaag ccgacccctt
     1381 cgtcatggcg gtgatggaag ccttcccggg cgcgaagatc ggcgacatca agacgctggc
     1441 cccggcggtg gaactgccgg ccatcccgga cgaggcgatg gacgaggact gacggcgccc
     1501 acgcccgagg acatctcgtt atggttgacg cgttaacaac acgctcttgt gagttgtggc
     1561 cggcgcgacg ccatgaggtc atccagtggt ggcgatcaag ccacctggga gcaagctaaa
     1621 gtgaaaaaga ttgttttggc ctcgatgttg gccgcgtccg ctattgtcac gatgccgacc
     1681 gccgccctcg ccgacacgga tgatgcgtgc atgctgcgca acggctgcgt ctgggcgagc
     1741 ggcaacgact ccaacccggg ctactggcag tgcagaagcc ccgccgtcta cgcgcggtgc
     1801 gtcagtcccg aaggagggcc tgaagacccc tcgctgacgt tcaagccgat caaggactga
     1861 agaccgggcg cggggggggg ggggg
//
LOCUS       DASVVN010000393         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_174209,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000393 DASVVN010000000
VERSION     DASVVN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174209"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF686_06325"
     CDS             <1..322
                     /locus_tag="VF686_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEX8661465.1"
                     /translation="CDGRASLVVGTHTHVPTADCQILPNGTAYQTDAGACADYDSVIG
                     NDKEEPLRRFTTRISGGRYTPASGPATICGVYVETDDRTGLATRVEPIRVGGRLSQAI
                     PVPV"
     gene            complement(324..524)
                     /locus_tag="VF686_06330"
     CDS             complement(324..524)
                     /locus_tag="VF686_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661466.1"
                     /translation="MGAVEGVHLSRTERSRRRRVRAEKIEAVLIVGLAALVLFKGAPT
                     IVGIALAIGEMAMDLIRLSGLV"
     gene            700..1764
                     /locus_tag="VF686_06335"
     CDS             700..1764
                     /locus_tag="VF686_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8661467.1"
                     /translation="MPAPGLAVAQDLTVIEEGPAVQPRAVQFIVRSRHADRHYLIQVT
                     PPARPPVFPGQKAAVVYALDGGWGVAGAAGWVLGGGGAMSPAYMVTIGYPPGQPSSRE
                     ADLLPRPVARQDGSMARGGGSDRFTRFLLEELKPFIEARYPVDPSRGVLFGHSLGGIF
                     TANVLADHPDAFAGYLIASPSVWADTTAPDRLRTISANGAGPRVFVGWGEREEPYMLE
                     GGRQVAAALAGRGDQLATHVFPGDLHITYYPAALGEALPFLLPRQAPLEFPLPVDVPE
                     DGLARLAGRYALQNGVEIGVAPTAQGLRATIPGMPPVDLKPRSPTSFFIPEMDVRVDF
                     EPNAMILFINGDRIDAVRIP"
     gene            1806..2276
                     /locus_tag="VF686_06340"
     CDS             1806..2276
                     /locus_tag="VF686_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8661468.1"
                     /translation="MFDPRVSRTGAPILLTALAVPGVASAAVRGITPANVFGDAAPSV
                     KLIMLLLVVGALAAVIVTVLKLAKGRRLSGGSAYVSALRLGGPLLGLMGAMFVLLMGF
                     IAMANVGEPVPMAVLAPGYAEAALLFLLGLLAGVIGVICHWIIEARIDRTVLGS"
     gene            2276..2599
                     /locus_tag="VF686_06345"
     CDS             2276..2599
                     /locus_tag="VF686_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8661469.1"
                     /translation="MTGRLHITEAESAVLAALWRRGPLSFASLIEEVKAANPWGDATI
                     KTLLHRLMQKGAVHSRKDDGRQRYHAVIDRQTYIEGEVQDLLDRLFDGKPDRLVALLA
                     DRPDL"
     gene            complement(2725..>3021)
                     /locus_tag="VF686_06350"
     CDS             complement(2725..>3021)
                     /locus_tag="VF686_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8661470.1"
                     /translation="HLDGRDPQGAPIGAEFVTTSISSSGDGQDQRADGRRYLADNAFL
                     KFNNAQRGYVVCDVTPERWQTEFKVLDKISDRNGVLTTRAKLVVASGDARIVAA"
BASE COUNT          454 a         1038 c          984 g          545 t
ORIGIN      
        1 ctgcgacggc cgcgcttcgc tggtggtggg cactcacacc cacgtgccga ccgccgactg
       61 ccagatcctg ccgaacggca ccgcctacca gaccgacgcg ggggcctgcg ccgactacga
      121 cagcgtcatc ggcaacgaca aggaagagcc gctgcgacgg ttcaccaccc gcatctcggg
      181 cgggcgctac acccccgctt cggggccggc cacgatctgc ggcgtctatg tcgagacgga
      241 cgacaggacc gggctggcca cgcgggtaga gccgatccgc gtcggaggcc ggctgagtca
      301 ggcaatcccg gttccggtct gatttatacg agaccgctca ggcggatcag gtccatcgcc
      361 atctccccga tagcgagcgc gatcccaact atggttggtg cacccttgaa aagcaccaga
      421 gcggccagcc cgacgatcag cacggcctcg atcttttcgg cgcgtacgcg ccgccgtctc
      481 gaccgctcgg tccggctcag gtgaacgcct tcaacggccc ccatgaagag tcctttcgct
      541 gcagccgtca caatgccgct tgacacgaaa gtttacaatc gtaatctaaa aagattacag
      601 acgtagaccg gccgtaaccc gtcctccggg ggctgtttca tcaggtggac catgcgcgtc
      661 ttcgccgttc ttaccatact cgcggcttcg atcgcagtga tgccggcgcc ggggctcgca
      721 gtagcccagg acctgaccgt gatcgaggaa ggtccagccg tacagccccg ggcggtgcag
      781 ttcatcgtcc gatcccggca cgcggatcga cactatctga tccaggtgac gccgcccgcg
      841 cggccgccgg tcttcccggg ccagaaggcc gcggtcgtct atgcgctgga tgggggttgg
      901 ggcgtcgccg gcgcggcggg ctgggtgctg ggcggtgggg gcgccatgtc gccggcctat
      961 atggtcacca tcggctatcc gccgggccag ccgagcagcc gggaagcaga ccttttgccc
     1021 cgccccgtcg ccagacagga tggaagcatg gcccgggggg gcggttcgga caggttcacc
     1081 cgcttcctgc tggaggaact gaaacccttc atcgaggcgc gctatccggt cgatccgtct
     1141 cgcggcgtgc tgttcggcca ttcgctgggg ggcatcttca cggcgaacgt tctggccgac
     1201 cacccggacg cgtttgcggg atacctgatc gccagtccct cggtctgggc cgacacgacg
     1261 gctccggacc ggctgcgcac catcagcgcc aatggcgccg gaccgcgcgt tttcgtcggc
     1321 tggggcgagc gtgaggagcc ctacatgctc gagggcgggc gtcaggtcgc ggccgcactg
     1381 gccgggagag gggaccaact ggctacgcac gtcttccccg gcgacttgca tatcacctac
     1441 tatccggcgg cccttggcga ggctctgccg ttcctgctgc cccgccaggc gccgctggag
     1501 tttccgctgc cggtcgatgt gccggaggac gggctggcca ggctcgccgg tcggtacgca
     1561 ctgcagaacg gcgtcgagat cggggtcgca ccgacggcgc aaggcctgcg ggccaccatt
     1621 cccggcatgc cacccgtcga tctgaaaccc cgctctccga ccagcttctt cattccggaa
     1681 atggacgtcc gcgtggactt cgaaccgaat gcgatgatcc tcttcatcaa cggcgaccgg
     1741 atcgacgcgg ttcggatccc ctgaccgctt gagcctgccc cgaattttca acccaaggag
     1801 tctccatgtt tgatccccga gtttcccgca ccggtgcgcc catccttttg acggcgcttg
     1861 cggtgcccgg cgtcgcctcc gctgcggttc gcggcatcac gcccgccaac gtgtttggcg
     1921 acgccgcgcc gagcgtgaag ctgatcatgc ttttgctggt cgtcggcgca ttggcggccg
     1981 tgatcgtgac cgtgctcaag ctggcgaaag ggcggcggct gtcgggcggg tccgcctatg
     2041 tctcggcctt gaggctgggc ggtccgctgc tggggctgat gggcgcgatg ttcgtgctgt
     2101 tgatgggctt catcgccatg gccaacgtcg gagagcctgt ccctatggcg gtgctggccc
     2161 cgggctacgc cgaggcggcc ctgctgttcc tgttgggcct gctcgcgggc gtcatcggcg
     2221 tgatctgcca ctggatcatc gaggcacgca tcgaccgcac cgtcctgggg tcctgatgac
     2281 cgggcgcctt cacatcacgg aggccgaaag tgcggttctg gccgcgttgt ggcgacgcgg
     2341 cccgctgtcc ttcgcctcgc tgatcgagga ggtgaaggcg gccaatccgt ggggcgacgc
     2401 gaccatcaag acccttcttc atcgcctgat gcagaagggg gccgtgcact cccggaagga
     2461 cgacggccgc cagcgctacc acgcggtcat cgaccgccag acctatatcg agggcgaggt
     2521 ccaggacctg ctggatcgcc tgttcgacgg caagcctgac cgcctcgtcg ccctgctggc
     2581 cgaccggccg gacctctgac gaaggctgcc ctgctgctgc ctccgtagcg tcgccgaagc
     2641 cgtagtcttc gtcgctgaat tcctcgaacc gggtcgtcga aatcccgccg aatccgtcgt
     2701 cgaattcctt gttgttgaaa tgcgtcaggc tgccacgatc cgcgcgtcgc cgctggccac
     2761 taccagcttc gcccgcgtcg tcagcacgcc gttcctgtcc gagatcttgt ccagcacctt
     2821 gaactcggtc tgccaccgct ccggcgtcac gtcgcagacg acatagccgc gctgggcgtt
     2881 gttgaacttc aggaacgcgt tgtcggccag atagcgccgg ccgtcggcgc gctggtcctg
     2941 gccatcgccc gacgagctga tcgaggtcgt cacgaactcg gccccgatcg gcgcgccttg
     3001 cgggtcgcgc ccgtccagat g
//
LOCUS       DASVVN010000394         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_409870,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000394 DASVVN010000000
VERSION     DASVVN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409870"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF686_06355"
     CDS             complement(<1..552)
                     /locus_tag="VF686_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEX8661471.1"
                     /translation="MTAQPRHSRRGLVVPFVIAGVLLAAWTGWWFYLTSQIETRLDAQ
                     VAALREDGWRISYASRTTTGWPFRARVSFPHAEIMAPSGHGIAAPELVAEANAYNPDR
                     WVVIAPDGLTLSRADKGKVGIAGDGLRLSVSHLRQRFPDLRVELIRPTFTAHQDAEPF
                     PIASAERILLNVRPHLTDGRAATD"
     gene            784..1218
                     /locus_tag="VF686_06360"
     CDS             784..1218
                     /locus_tag="VF686_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8661472.1"
                     /translation="METQSLHRGRLIDHIQLVVRDLDATRRFYDAVFEVLGMPLGGEG
                     DNYFWVDELFISTADSEAAAGKLTGRHHLAFQAQDRAMVDAFHAAGCAAGGKDNGAPG
                     ERPYHPGYYAAFLLDPDGNNIEAVFHGEAERSAKSVEITFEQ"
     gene            complement(1366..>3398)
                     /locus_tag="VF686_06365"
     CDS             complement(1366..>3398)
                     /locus_tag="VF686_06365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX8661473.1"
                     /translation="NQGAAWVSTYTRALQRQIDRESAALWPDPVERKRKTVVRKGREN
                     YLCLLNLQEMVQAAQLGNGDLIGMALASRWAMHSRDGDMTGGDYPGWLPGLFATGPSH
                     QASAANLVDRRGECVHAACPHYRTCFVEKTIRASRRADLVVANHALVMTQAAFDGARS
                     ARGLKQDGETAALKRIVFDEGHHLFDAADSAFSACLSGQEAAELRRWLRGPEGRGRRG
                     RGLEQRLGDLCADNEAATKALQDAVRAAAQLPGEGVSGRIAPASGEVNPVGPIERFLL
                     AALEQLRARTSENASGSGVEFGMECSLRPVTEPVLEAAREAAQALAAVEAPLLALSRH
                     LEDILDDEAADLDGASRARIEGALRGLDRRARMTLPGWRSMLAALEVSSDEPDPDFLD
                     WLSAEAAFGRIMDVALRRHWIDPTVPLEAAVIMPAHGVLVTSATLSDPNSDDPFDMAR
                     LRTGSARLIEPARTLKVESPFDYAANSRVIVVNDLMKDDTRQTAAAMRELFLAAGGGG
                     LGLFTAIRRLKAVYERLGPDLAKQGIPLYAQHVDPLEVGALVDVFRAEQDSCLLGTDA
                     VRDGVDVPGRSLRILAFDRVPWPRPDLLHKARRLRFGGEGAGGRNFDDALARARMAQA
                     FGRLIRRADDKGVFVMLDAAAPTRLFASLPPGTEVQRMGLAEAVETVAAFLA"
BASE COUNT          512 a         1119 c         1275 g          492 t
ORIGIN      
        1 gtcggtcgcg gcgcggccgt cagtcaggtg cggccggacg ttgagcagga tccgctccgc
       61 cgaggcgatg gggaagggtt cggcgtcctg atgggccgtg aaggtcggtc ggatcagctc
      121 gacgcgcaga tcggggaagc gttggcgcag atggctgacg ctgaggcgca ggccgtcgcc
      181 ggcgatgccg accttgccct tgtcggcgcg gctcagtgtc aggccgtccg gggcgatgac
      241 cacccagcgg tcagggttgt aggcgttggc ctcagcgacc agttccggcg cggcgatccc
      301 atggccggag ggggccatga tctcggcgtg ggggaaggac acgcgggcgc ggaagggcca
      361 gccggtggtg gtccgcgagg cgtagctgat gcgccagccg tcctcgcgca gggcggcgac
      421 ctgggcgtcg agccgcgtct cgatctgact ggtcagatag aaccaccagc ccgtccatgc
      481 ggccagcagc accccggcga tcacgaaagg aacgacaagg ccgcgtcggc tgtggcgagg
      541 ttgggcggtc atcgaggctc cgggaagagg ggtctgaaca gttcgaacgt ctgaagcccg
      601 gaaccgggcc gaaaaaacag tctacagcgt ctacagagtc tactgcgtcc attcctttcg
      661 ggggccgcgg cgtcttcgcc gggggtccga tatggtgaag cctcggcact ccggcaagcc
      721 tggaagcgga acatcggatc gggtcaatcc gttcgatccg gattcacaac gcgggagccc
      781 gtgatggaaa cgcagtcgct gcaccggggt cgactgatcg accacatcca gctggtggtg
      841 agggatctgg acgccacgcg ccgcttttac gacgcggtgt tcgaggtgct gggcatgccg
      901 ctcggtggcg agggcgacaa ctatttctgg gtcgacgagc tgttcatctc gaccgcggac
      961 agcgaggcgg cggcgggcaa gctcactggc cggcatcatc tggcgttcca ggcgcaggac
     1021 cgggcgatgg tggacgcctt ccatgcggcg ggatgcgccg cgggcggcaa ggacaacggg
     1081 gcgccgggcg agcggcctta tcacccgggc tactatgcgg cgtttctgct ggaccccgac
     1141 ggcaacaata tcgaggcggt gtttcacggc gaggccgagc gctcggcgaa gagcgtggag
     1201 atcacgttcg aacaataagt ctcctccctg ttcgcgcagc gaatgggagg tggcgcggcg
     1261 cggcttcgcg ccgtgacgga ggggtagaag cagggacgcg gtctggcgcc cctccaccgc
     1321 ttcgcggtcc ccctccccat cgcctgcggc gacagggagg agacttcagg cgaggaacgc
     1381 cgccaccgtc tccaccgcct cggccagacc catgcgctgg acctccgtgc ccggggggag
     1441 gctggcgaac aggcgggtgg gggccgcggc gtccagcatg acgaagaccc ccttgtcgtc
     1501 ggcgcggcgg atcagccggc cgaacgcctg ggccatgcgg gcacgggcga gggcgtcgtc
     1561 gaaattgcgg cccccggcgc cctcgccgcc gaagcgcagg cggcgcgcct tgtgcagcag
     1621 atcgggacgc ggccacggca cgcggtcgaa ggcgagaatg cgcagggacc ggcccggcac
     1681 gtcgacgccg tcgcgcacgg catcggtgcc cagcaggcag gagtcctgtt cggcgcgaaa
     1741 gacgtcgacg agggcgccga cttccaatgg gtcgacgtgc tgggcgtaga gggggatgcc
     1801 ctgtttcgcc agatcgggac ccagccgctc atagacggcc ttcagccggc ggatcgcggt
     1861 gaacaggccg aggcccccgc cgcccgcggc gaggaagagt tcgcgcatgg ccgcggcggt
     1921 ctggcgcgtg tcgtccttca tcaggtcgtt gacgacgatg acccggctgt tggcggcgta
     1981 gtcgaacggg ctttcgacct tcagcgtgcg cgcaggctcg atcagccgcg ccgatccggt
     2041 tcgcaggcgg gccatgtcga acggatcgtc cgagttcgga tcggacaggg tggcgctggt
     2101 caccagcacg ccgtgggcgg gcatgatgac ggcggcttcc agcggcacgg tcggatcgat
     2161 ccagtggcgg cgcagggcca cgtccatgat gcggccgaag gcggcctcgg cggacagcca
     2221 gtcgaggaaa tcggggtccg gttcgtcgga cgacacctcc agcgccgcca gcatggagcg
     2281 ccatcccggc agggtcatgc gggcgcggcg gtcgagaccg cgcagggcgc cctcgatacg
     2341 ggcgcgcgac gccccatcca gatccgccgc ctcgtcgtcg aggatgtcct ccagatgccg
     2401 cgacagggcc agcagcggcg cctcgacggc ggcgagggcc tgggcggcct cgcgggcagc
     2461 ttccagcacc ggctcggtca ccgggcgcag cgagcactcc atgccgaact cgacgccgct
     2521 tccggaggcg ttctcgctgg tccgtgcacg gagctgttcc agcgcggcca gcagaaagcg
     2581 ttcgatcggc ccgaccggat tgacctcgcc cgaggccgga gcgatgcggc ctgagacgcc
     2641 ctcgccgggc agctgggcgg cggcgcggac ggcgtcctga agcgccttgg tcgcggcctc
     2701 gttgtcggcg cagaggtcgc ccagccgctg ttccaatccg cggccgcgac ggccgcggcc
     2761 ctcgggcccg cgcagccagc ggcgcagctc ggcggcctcc tgaccggaca gacaggccga
     2821 gaaggcgctg tcggcggcgt caaacaggtg gtggccctcg tcgaagacga tccgtttcag
     2881 ggccgccgtc tcgccgtcct gtttcaggcc gcgcgccgag cgggcgccgt cgaaggcggc
     2941 ctgggtcatg acgagggcgt ggttggccac gaccagatcg gcgcggcggc tggcgcggat
     3001 ggtcttttcc acgaaacagg tgcggtagtg cggacaggcg gcgtggacgc attcgccgcg
     3061 ccggtcgacc agattggcgg cgctggcctg atggctgggc cccgtcgcga acaggccggg
     3121 cagccagccc ggatagtcgc cgccggtcat gtcgccgtcg cggctgtgca tggcccagcg
     3181 gctggccaag gccatgccga tcaggtcgcc gttccccagc tgggccgcct gaaccatctc
     3241 ctgcagattc agcaggcaca ggtagttctc ccgccccttg cggacgaccg tcttgcgctt
     3301 gcgctcgacc ggatcgggcc acagcgctgc gctctcgcgg tcgatctgcc gctgcagggc
     3361 gcgggtatag gtcgacaccc aggccgcccc ctgattgc
//
LOCUS       DASVVN010000395         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_251429,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000395 DASVVN010000000
VERSION     DASVVN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251429"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..779)
                     /locus_tag="VF686_06370"
     CDS             complement(150..779)
                     /locus_tag="VF686_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8661474.1"
                     /translation="MSGTVNVLQEGVRLLFADDDPILREFASVHLGAEQVSTAEDGED
                     AWRMVQAAPFDLLLIDLEMPRLDGFGLVQRLRADPRFRDIPVIVVTGRDDVNAIDRAF
                     QAGATSFLVKPINWRLLTYQVRFVLRAQRAMASLHAASQAPAAAAAAPSGPAPGAMLR
                     AVLTESTPLLTLGMQGDPAMQAATERFVALLDRLSREEHAAYLGSDRAA"
     gene            893..>2791
                     /locus_tag="VF686_06375"
     CDS             893..>2791
                     /locus_tag="VF686_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8661475.1"
                     /translation="MSLEAKKSLSGGPVGGAPRLRRTIRSKLIVLVITPLALAVALIT
                     AMSTWRAAAGEAALESDRLNYAAATLAAFASDAVQAQDPEQAFAALRAIAQIDDVSYA
                     RLETVDGRVLAETGAGARLVRDASLDAGRGLSFSDLFGTQTIRVTAPVLHGGRPVGAV
                     VMLSQTESAVSRARAGLAVTGFGAGAAAILGLLVAFRLQRGISAPIVGLTQAMSSVRA
                     SHDYALEAELKSDDEIGDLVEGFNQMLHEIRDRDARLAGYTQTLEREVADRTAEIRVA
                     KDIAEAANASKSEFLATMSHEIRTPMNGIMVMAEMLASADIPQRQRRYAQVIAKSGQS
                     LLSIINDILDFSKIEAGKMELESEPVDLADLVDDVTSLFWERARSKSIDLAAYVDPNT
                     PKKVLGDSVRLRQVIGNLVNNAIKFTETGGVLIRVEAAKDNVIRVAVQDTGIGIPQEK
                     IAGLFEAFTQADQSTTRRFGGTGLGLAICKRLIDAMGGRIGASSEPGKGSTFVFVIPA
                     ETVEAADPWPDMSGAELNRAAVVCAGLSTRWALVSYLQAAGVELDAGAPDFFIGEPHG
                     YGALPRPAICLAEYGDPEPFDRLRARALDAVLVQPFRRKELAAILVRLRDGQSLEDLQ
                     VDDGPAGAV"
BASE COUNT          438 a         1006 c          960 g          387 t
ORIGIN      
        1 acatcccacg gttgccgctc tcacggcgtt cggcagtgcg gttggattcg gtattgctca
       61 tctcgttccc agccaaggtt gcccacgcca aacgaggcgt aacagcaaag gttccagacg
      121 gggcccggcc cgtcaatggc caagcttggt caggccgccc ggtccgaacc gaggtaggcg
      181 gcgtgctcct cgcgggagag gcggtcgagc agggcgacga agcgctcggt cgccgcctgc
      241 atggcgggat cgccctgcat gcccagggtg aggagcggcg tgctctcggt cagcaccgcg
      301 cgcagcatgg cccccggggc aggtccggac ggagctgcgg cggcggccgc cggcgcctgg
      361 ctggcggcgt gcagcgacgc catcgcccgc tgggcgcgga ggacgaagcg gacctggtag
      421 gtcagcaacc tccagttgat cggcttgacc aggaaggagg tggcgccggc ctggaaggcg
      481 cggtcgatgg cgttcacgtc atcgcggccg gtgaccacga tgacgggtat gtcgcggaag
      541 cggggatcgg cgcgcagccg ctggaccagg ccgaagccgt ccaggcgggg catctcgagg
      601 tcgatcagca gcaggtcgaa gggcgcggcc tggaccatcc gccaggcgtc ctcgccgtcc
      661 tcggcggtgc tcacctgctc ggccccgagg tgcacggagg cgaattcgcg caggatgggg
      721 tcatcgtcgg cgaacaggag ccgaaccccc tcctgcagga cgttaaccgt cccgctcacc
      781 tctgtggggg cagccgacat gcacgctccg ggcctcgaat cccccagcat taagctggag
      841 gtcattaatc ccccgtaact ggaaggggtt ggcccccgcc tgagaagccc gtgtgagcct
      901 ggaagccaag aagagcttga gtgggggacc ggtgggaggc gcgccgcgcc tgcgccgcac
      961 gatccgctcc aagctcatcg tgctggtgat cacgcccctg gccctggccg tggccctgat
     1021 caccgccatg tcgacctggc gcgccgccgc cggcgaagcc gcgctggaaa gcgaccggct
     1081 gaactatgcg gccgccaccc ttgccgcctt cgcctccgac gcggttcagg cccaggatcc
     1141 cgagcaggcc ttcgcggccc tgcgggccat cgcccagatc gacgacgtca gctacgcccg
     1201 gctggagacg gtggacggcc gcgtcctggc cgagaccggc gccggcgccc ggctggtccg
     1261 cgacgccagc ctggacgctg ggcgaggcct gtccttctcc gacctgttcg ggacccagac
     1321 catccgcgtc accgccccgg tgctgcacgg cggccggccc gtcggcgcgg tggtcatgct
     1381 cagccagacc gagagcgccg tcagccgcgc ccgggcaggc ctcgccgtca ccggcttcgg
     1441 cgccggcgcc gccgccatcc tcggcctgct ggtggccttc cggctgcagc gcggcatctc
     1501 cgcgccgatc gtgggcctga cccaggccat gtcgagcgtg cgggcctcgc acgactatgc
     1561 gctcgaagcc gagctgaaga gcgacgacga aatcggcgac ctggtcgaag gcttcaacca
     1621 gatgctgcac gagatccgcg accgtgacgc gcggctcgcc ggctacaccc agacgctgga
     1681 gcgcgaggtc gccgaccgca ccgccgagat ccgcgtcgcc aaggacatcg cggaagccgc
     1741 gaacgcatcc aagtccgagt tcctggccac catgagccac gagatccgga cgccgatgaa
     1801 cggcatcatg gtgatggccg agatgctggc ctcggccgac atcccgcagc gccagcgccg
     1861 ctacgcccag gtgatcgcca agtcgggcca gagcctgctg tccatcatca acgacatcct
     1921 cgacttctcc aagatcgaag ccggcaagat ggagctggag tccgagccgg tcgacctggc
     1981 ggacctggtc gacgacgtca ccagcctgtt ctgggagcgc gcacgctcca agtcgatcga
     2041 cctcgcggcc tacgtcgacc cgaacacccc gaagaaggtg ctgggcgact ccgtgcgcct
     2101 gcgccaggtg atcggcaacc tggtcaacaa cgccatcaag ttcaccgaga ccggcggcgt
     2161 gctgatccga gtcgaggcgg ccaaggacaa cgtcatccgc gtggccgtgc aggacaccgg
     2221 catcggcatc ccgcaggaga agatcgccgg cctgttcgaa gccttcaccc aggccgacca
     2281 atcgaccacg cgccgcttcg gcggcaccgg cctgggcctg gccatctgca agcggctgat
     2341 cgacgccatg ggaggtcgca tcggcgcctc cagcgaaccc ggcaagggct cgaccttcgt
     2401 cttcgtcatt ccggcggaga ccgtcgaggc ggccgatccg tggcccgaca tgtccggcgc
     2461 cgagctcaac agggcggcgg tggtgtgcgc gggcctgtcg acccgctggg cgctggtcag
     2521 ctacctccag gccgccggcg tcgagctcga cgcgggcgcg cccgacttct tcatcggcga
     2581 gccgcacggc tacggcgccc tgccccgccc cgcgatctgc ctcgccgagt acggcgatcc
     2641 cgagccgttc gaccggctgc gcgcacgcgc gctcgacgcc gtgctggtgc agcccttccg
     2701 gcgcaaggag ctcgcagcca tcctcgtgcg cctgcgcgac ggccagtcgc tggaagacct
     2761 gcaggtcgac gatggacccg ccggcgcagt c
//
LOCUS       DASVVN010000396         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_462683,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000396 DASVVN010000000
VERSION     DASVVN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462683"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1254
                     /gene="lpxB"
                     /locus_tag="VF686_06380"
     CDS             88..1254
                     /gene="lpxB"
                     /locus_tag="VF686_06380"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX8661476.1"
                     /translation="MSRPLKIMLVAAEASGDALGAGLAEALKARLGPDVVFVGVGGPR
                     MAEQGVQSPFDIAELSILGWLEGLKAYGVVKRRVADTAALAVAEKPDAVVLIDSWGFT
                     IRVAKALKAALPGLPLIKYVGPQVWASRPGRAKTLAASVDHLLALYAFDAPWFEREGL
                     PTTVVGSQALHVDFGDADPAAFRASRGMASDPNLLLVLPGSRPSEIRLMTPVYEAAVA
                     RLKAEDPALEVAVVAAGTVADDVAARVAGWPFRAHLVAESEKYAAMRAATVALATSGT
                     VSTELALAGAPMVISYRFQPLSYAIMKPFFTGTYATLINHAADAEIARELIQNDATPE
                     KLATEVGRLLADPAARRAQAERQTAALDLMGREGRDPSEIAADAVLRVIASKAA"
     gene            complement(1251..2336)
                     /locus_tag="VF686_06385"
     CDS             complement(1251..2336)
                     /locus_tag="VF686_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8661477.1"
                     /translation="MPHGRFRFERFQLDTGDRLLTRDEVRVELNARYFDALTLLVREN
                     GKLVSKDRFLDEVWRGVPVTDEALTQCIRTIRRQLGDDASRPRFIDTVPKHGYRFIAP
                     VTRDDSEPGPMAAVPGQTPTATPHGRWPLFLRLGAAGMIGGGIAGVFGGLFYGLIAAS
                     QPQQSGMGAVSVILVLICLTAGVGLLGGAGVGFGVAAAGFASGRAWLWNTIGGAVGGM
                     IVGAVVKLIGIDAFSLLFGQSPGAITGAAEGAILGGAIGFGAWLGPRIGGPSSPAHSV
                     AAAGLAGGTAGALIPLLGGRLMGGSLQQLADGFPNSRLGIDRMGVLFGESSFGLTSQI
                     ITAGLEGAMFGAWVAVALRLARRRPGV"
     gene            complement(2495..>3636)
                     /gene="gltA"
                     /locus_tag="VF686_06390"
     CDS             complement(2495..>3636)
                     /gene="gltA"
                     /locus_tag="VF686_06390"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8661478.1"
                     /translation="TAACESALTFIDGDVGTLLHRGYPIDQLASQSNFIEVCHLLLHG
                     ELPTASEYEAFERNITMHTMLHAQFDRFFEGFRRDAHPMAIMVGTVGALSAFYHDSLD
                     IHDPVQRNISAIRLIAKMPTIAARAYKYHIGQPFVSPRNDLTYAENFLRMCFAVPAED
                     YEVNPVLARAMDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPSHGG
                     ANEEALNMLKEIGTPDKIPEFIEGVKAKKYKLMGFGHRVYKNYDPRAKVMKESADEVL
                     AAVGDPNDPLFQVAKELERIALNDEYFIERKLFPNVDFYSGITLSAMGFPTSMFTVLF
                     SLARTVGWIAQWQEMMADPGQKIGRPRQLYTGPTERDYVPIAKRG"
BASE COUNT          571 a         1181 c         1330 g          554 t
ORIGIN      
        1 ggccgggaaa ctgatcctga tcgaccacgc cgccatcgtc gaggccgccg atcgcctggg
       61 tttgttcgtg tggggcgact gaagcgaatg agccggccgc tgaagatcat gctggtggcg
      121 gccgaggcct cgggcgacgc cctgggcgca ggcctggccg aggcgctgaa ggcgcggctg
      181 ggccccgacg tggtctttgt cggcgtgggc ggtccgcgga tggccgaaca gggcgtgcag
      241 agcccgttcg atatcgccga actgtccatt ctcggctggc tcgagggcct gaaggcctat
      301 ggcgtggtca agcgccgggt cgcggatacc gcagccctgg ccgtcgcgga gaagccggac
      361 gccgtggtcc tgatcgacag ctggggcttc accatccgcg tggccaaggc gctgaaggcg
      421 gcgctgccgg gcctgccact gatcaaatac gtcgggccgc aggtctgggc ctcgcggccg
      481 ggccgggcga agacgctggc ggcgtcggtc gatcacctgc tggccctcta tgccttcgac
      541 gcgccatggt tcgagcgcga ggggctgccg accacggtgg tgggttcgca ggcgctgcat
      601 gtcgatttcg gcgatgcgga cccggccgcc ttccgggctt cgcggggcat ggcgtccgat
      661 ccgaacctgc tgctggtcct gccgggtagc cgtccctctg aaatccggct gatgacgccc
      721 gtgtacgaag ccgccgtagc acggctgaag gcggaggatc cggcgttgga ggtcgcggtc
      781 gtggcggcgg gcacggtggc cgacgacgtc gccgcacgcg tggccggctg gccgttccgg
      841 gcgcatctgg tcgccgagag cgagaaatac gccgccatgc gcgccgccac cgtggccttg
      901 gccaccagcg gcacggtctc gaccgaactg gcgctggccg gggcgccgat ggtgatctcc
      961 taccggttcc agccgctcag ctatgcgatc atgaagccgt tcttcaccgg cacgtacgcg
     1021 acccttatca accacgccgc tgacgccgag atcgcccgcg agctgatcca gaacgacgcc
     1081 acgccggaaa agctggcgac ggaggtcggc cggttgctgg cggacccggc ggcgcggcgt
     1141 gcgcaggcgg agcggcagac ggcggcgctg gacctgatgg gccgggaggg gcgcgatccg
     1201 tccgagatcg ccgccgacgc cgtgctgcgg gtcatcgcga gcaaggcggc ctagacgccg
     1261 gggcgtcgcc gcgccagccg cagggcgacc gccacccagg cgccgaacat ggcgccttcc
     1321 agaccggcgg tgatgatctg gctggtcagg ccgaaactgc tctcgccgaa caggacgccc
     1381 atcctgtcga tgccgagccg ggagttggga aagccgtcgg ccagctgctg caggctgccg
     1441 cccatcagcc ggcctcccag cagggggatc agcgcgccgg ccgttccacc ggccagaccg
     1501 gcggcggcga cgctgtgggc cggcgaggag ggacctccga tccggggccc cagccaagcg
     1561 ccgaaaccga tcgcgccgcc cagtatggcg ccctcggccg cgcctgtgat cgcgccgggg
     1621 ctttgaccga acaggaggct gaaggcgtcg atgccgatca gcttgacgac cgccccgacg
     1681 atcatgcccc cgacggctcc gccgatcgtg ttccacagcc aggcccggcc cgaggcgaaa
     1741 cccgcggcgg cgacgccgaa gccgacgccg gctccgccca gcaggccgac gccggccgtg
     1801 agacagatca gaacgaggat gaccgacacc gcgcccatgc cggactgctg gggctgggag
     1861 gcggcgatca ggccgtagaa caggccgccg aagacgccgg cgatcccgcc gccgatcatc
     1921 cccgccgcgc cgagccggag gaagagcggc cagcggccgt gcggcgtcgc tgtcggcgtc
     1981 tgacccggga cggccgccat cgggccgggt tcggagtcat cgcgcgtgac gggcgcgatg
     2041 aagcggtagc cgtgcttggg aaccgtatcg atgaaacgcg ggcgggatgc gtcgtcgccc
     2101 agctgccggc ggatcgtgcg gatgcactgg gtcagggcct cgtcggtgac gggcacgccg
     2161 cgccagacct cgtcgaggaa gcgatccttg gaaaccagct tgccgttctc gcgaaccagc
     2221 agggtgaggg cgtcgaaata gcgggcgttc agctcgaccc ggacctcgtc ccgcgtcagc
     2281 agccggtcgc cagtgtcgag ctggaagcgt tcgaagcgaa agcggccatg cggcatgtgt
     2341 tcagaaggac cccggattcg cgcagcgtcc cccatcaggg tcggcaaggc aatgggctga
     2401 caacccacga aaaacgccgg ggcgcgggcc ccggcgtttg cattcctctg ggccgcggtc
     2461 agaaggctcc gccttctgga cccgacgcgg cctgtcagcc ccgcttggcg atcggcacgt
     2521 agtcccgttc ggtcgggccg gtgtagagct ggcgcgggcg gccgatcttc tggccggggt
     2581 cggccatcat ttcctgccac tgggcgatcc agccgacggt gcgggccagc gagaacagca
     2641 cggtgaacat cgaggtcggg aagcccatcg ccgacagggt gatgcccgaa tagaaatcga
     2701 cgttcgggaa cagtttccgc tcgatgaaat actcgtcgtt cagcgcgatg cgctccagct
     2761 ccttggcgac ctggaacagc gggtcgttgg ggtcgccgac ggcggccagc acctcgtcgg
     2821 cgctctcctt catcaccttg gcgcgcgggt cgtagttttt gtacacgcgg tgaccgaagc
     2881 ccatcagctt gtatttcttg gccttcacgc cctcgatgaa ctccgggatc ttgtccgggg
     2941 tgccgatctc cttgagcatg ttcagcgcct cttcattggc gcccccatgg ctggggcccc
     3001 acaggcaggc gatgccggcg gcgatacagg cgaagggatt ggcgccggac gaaccggcca
     3061 gacggacagt cgaggtcgag gcgttctgct cgtggtcggc gtgcaggatg aagatgcggt
     3121 ccatggcgcg ggccagaacc ggattgacct catagtcctc ggccggaacg gcgaagcaca
     3181 tgcgcaggaa attctcggcg taggtcaggt cgttgcgcgg cgacacgaag ggctggccga
     3241 tgtggtattt gtaggcgcgc gccgcgatgg tcggcatctt ggcgatcagg cggatggccg
     3301 agatgttgcg ctggaccgga tcatggatgt ccaggctgtc gtgatagaag gccgacaggg
     3361 cgccgaccgt gccgaccatg atcgccattg ggtgggcgtc gcggcggaag ccctcgaaga
     3421 agcggtcgaa ctgcgcatgc agcatggtgt gcatggtgat gttgcgctcg aacgcttcgt
     3481 attccgaggc cgtcggcagt tcgccgtgca gcagcaggtg gcagacctcg atgaagttgg
     3541 actgcgacgc cagctggtcg atcggatagc cgcggtgcag cagggtgccg acatcgccgt
     3601 cgatgaaggt cagggcgctt tcgcaggcgg cggtcg
//
LOCUS       DASVVN010000397         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_462691,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000397 DASVVN010000000
VERSION     DASVVN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462691"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VF686_06395"
     CDS             complement(<1..598)
                     /locus_tag="VF686_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661479.1"
                     /translation="MFEFGRDLRKLFDKARESDDLGWLELIGADLVEAEARRETIDAG
                     RVSCTQPFTGWMRASALWREHARRTGRRTSLDRATAAASDAARHTTTSDQTASAAIEA
                     GEIHLLHFDLFGGPEKLTAALSDIQSVCAERPAVQSGAAALHARLTARRSRLSGEPSV
                     MLDAAALLDAAVHGARGLPQTMGDELMLDRAALSLEAGV"
     gene            complement(685..1941)
                     /locus_tag="VF686_06400"
     CDS             complement(685..1941)
                     /locus_tag="VF686_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX8661480.1"
                     /translation="MPPVVRYLVLALALSALSPAAALADWRRAESAHFIVYSDGSERS
                     LRDYTAKLERFDALLTARFGVGDAAEARRLPVYLVSDARALRVAIPGLPPGIAGYYSS
                     SDIDTFAVLVRGESDDILLHEYSHHVMARAGDMRYPGWFREGFAEYFATATVSEGGAA
                     SFGLPDRGRLYALQQGRWLPMDAVLRADSPLAIDNQTGMNMYYAQSWALTHWLLSDTA
                     RIRNLSAYVAAVNGGRDPVEAWQAIYSITPEQLTAQLRAHVRGRLSYSKLDMPPISPV
                     ITVTTLSPAADAVMLPAINARSWDPGQIDGPALLQTLRTAAARFPDDPLALVAAGRAE
                     KRGGDEAAAETALTRALERQGDNVEGLILMADLATERGADATDPAERTRQWDLARDFL
                     RRALEADPTDGRVYSAQNRFRRYARD"
     gene            complement(2040..>3113)
                     /locus_tag="VF686_06405"
     CDS             complement(2040..>3113)
                     /locus_tag="VF686_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661481.1"
                     /translation="AWFREGFAEFFMTATVTPNGRASVGKSSLMRIRTLNNIPWLPME
                     QVLTGQYERGESGKHAAFYAQSWLLTHWILTDPERRRRMQAWLGDTARGVAPEAALRT
                     HFDMDVRQLERTLRAYLDAGPRYAELEVGGAEPTLTVTALPDSADDVLLLDLNARDGG
                     PADQGPDMLASAREAAADHPGDALAQMMLARVELRWGDPAAAERAVQPLLDANPANTD
                     LLLLMADARITAGDKLTDPEERERLYRQARGFLARAYAADPGDNRIYQNIAYSRRFAP
                     DYPTENDVELWRRAVAIAPEVASNRAVTAEAMLRRGLVDEAEQVILPVANDPHGGERP
                     ERLQRILDRIAELRAGAAPAPAN"
BASE COUNT          470 a         1066 c         1108 g          469 t
ORIGIN      
        1 cgacgccggc ttcaagcgac aatgcggcgc ggtccagcat cagctcgtcg cccatggtct
       61 gaggcaggcc ccgggcgccg tggacggcgg cgtccagcag ggccgccgca tccagcatga
      121 ccgacggctc gccggacagc cgcgaccggc gcgccgtcag tcgcgcgtgc agcgccgcgg
      181 cgcctgactg gactgcgggg cgttcggcgc agacggattg aatatcggac agggccgccg
      241 tcagcttctc gggtccgccg aacaggtcga aatgcagcag atgaatttcg ccggcctcga
      301 tcgccgccga ggcggtctgg tcagacgtgg tcgtatgccg ggcggcgtcg gatgcggcgg
      361 cggtcgcgcg gtccaggctg gtccgccggc cggtgcgccg ggcgtgctcg cgccacaggg
      421 ccgaggcgcg catccagccg gtgaaaggct gggtgcagga gacgcggccg gcgtcgatgg
      481 tttcacgccg ggcctcggcc tcgacgaggt cggcgccaat gagttccagc cagccgaggt
      541 cgtcgctctc gcgcgccttg tcgaacagct tcctcaaatc ccggccgaat tcgaacatga
      601 gatcaggtcg tcccgcttcg ggaccgaaaa ggtgtcccga ggtacaggat gcaaacgcag
      661 cgccgccgtc agggttctat ccgctcaatc gcgcgcgtac cggcggaacc ggttctgcgc
      721 ggaatagacc cggccgtcgg tgggatcggc ttccagggcg cggcgcagga aatcccgcgc
      781 aaggtcccac tgtcttgtcc gctcggccgg gtccgtcgca tcggccccgc gctccgtggc
      841 cagatccgcc atcaggataa gcccctcgac attgtcgccc tgacgctcca gcgcacgggt
      901 cagggccgtc tccgccgcgg cctcgtctcc cccgcgcttt tcggcgcggc ctgctgcgac
      961 cagagccaag gggtcgtcgg ggaagcgggc ggcggccgtc cgcaaggttt gcagaagcgc
     1021 ggggccgtcg atctggccgg gatcccacga gcgggcgttg atcgccggca gcatgactgc
     1081 atcggccgcg ggcgaaaggg tcgtcacggt gatgaccggg ctgatggggg gcatgtcgag
     1141 cttgctgtag gagagccgcc cgcgcacatg ggcccgaagc tgggccgtca gctgctccgg
     1201 agtgatggaa tagatggcct gccaggcctc gaccgggtcc cggccgccgt tcaccgcggc
     1261 gacataggcg gacaggttcc ggatccgggc cgtgtccgac agcagccagt gagtcagcgc
     1321 ccaggactgg gcgtagtaca tattcatgcc cgtctgattg tcgatcgcca gaggactgtc
     1381 ggcccgcagc accgcgtcca tcggcagcca gcggccctgt tggagggcat acagccgccc
     1441 ccggtcgggc aggccgaagg acgccgcccc gccctcgctc accgtcgccg tggcgaaata
     1501 ttcggcaaag ccttcgcgga accatcccgg ataccgcatg tcgccggccc gggccatgac
     1561 atgatggctg tactcgtgca gcaggatgtc gtcgctctcg ccccgaacca gcacggcgaa
     1621 ggtgtcgata tcgctgctcg aatagtagcc tgcgatcccg ggcggcaggc cgggaatggc
     1681 cacgcgcaac gcccgggcgt cggagaccag atagaccggc agcctgcggg cctcggccgc
     1741 atccccgacg ccgaagcggg ccgtcagcag ggcgtcgaaa cgctccagct tggcggtgta
     1801 gtcgcgcagc gaccgttcgg agccgtcgct gtagacgatg aagtgcgcac tttcggcgcg
     1861 ccgccagtcg gcaagagccg cagccggcga cagggcgctc agcgcaagcg cgagaacaag
     1921 ataacggaca actggcggca acacggaact ccccccggaa tgtccatcat tctaagcccg
     1981 gtttgcgccg cctggtccag ccacagattg cccggcgcgc ctttaccggc gcgggcggat
     2041 cagtttgccg gcgcgggcgc cgcaccggcg cggagctccg cgatccggtc gaggatgcgc
     2101 tggagccgtt ccggtctttc gccgccgtgg ggatcgttgg cgacaggcag gatgacctgt
     2161 tccgcctcat cgacgaggcc gcgccgcagc atggcctcgg cggtcacggc gcggttgctg
     2221 gcgacctcgg gcgcgatggc gacggcgcgg cgccacagtt cgacgtcgtt ttcggtcgga
     2281 tagtccgggg cgaaacgccg gctgtaagcg atgttctgat agatgcggtt gtcgccgggg
     2341 tccgccgcat aggcgcgcgc caggaagccc cgcgcctgac gatacaggcg ctcgcgttct
     2401 tccggatcgg tcagcttgtc gccagcggtg atccgcgcgt cggccatcag caacagcagg
     2461 tccgtattcg ccggattggc gtccagcagg ggctggacgg cccgttccgc cgcggccggg
     2521 tcgccccagc gcagttcgac ccgcgccagc atcatctgcg ccagggcgtc gcccggatga
     2581 tccgccgccg cctcgcgggc gctggccagc atgtccggcc cctgatccgc ggggccgccg
     2641 tcccgggcgt tgagatcgag cagcagcacg tcgtcggcgc tgtcggggag ggcggtgacg
     2701 gtcagggtcg gctcagcacc gccgacctcc agttcggcgt atcgcgggcc ggcgtccaga
     2761 taggccctga gcgtccgctc cagctgccgg acatccatat cgaaatgcgt ccgcagggcc
     2821 gcctcaggag ccacgccccg cgctgtatcg ccgagccagg cctgcatgcg ccggcggcgc
     2881 tcggggtcgg tcaggatcca gtgcgtcagc agccacgact gggcgtagaa ggccgcgtgt
     2941 ttgccggact cgccgcgctc atactggccg gtcagcacct gctccatcgg cagccaggga
     3001 atgttgttga gggtccggat ccgcatcagg ctgctcttac cgaccgaggc gcggccgttg
     3061 ggggtcacgg tcgcggtcat gaagaactcg gcgaaccctt cgcggaacca ggc
//
LOCUS       DASVVN010000398         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_251447,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000398 DASVVN010000000
VERSION     DASVVN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251447"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>1557
                     /gene="glnD"
                     /locus_tag="VF686_06410"
     CDS             31..>1557
                     /gene="glnD"
                     /locus_tag="VF686_06410"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX8661482.1"
                     /translation="MTPAPDDRLTEAASAPDVRKAVAEILRESFDAARAKAARRLAAG
                     GDGVEVARLSSAAADDLLTALWRFTTETLYPAYNPTEAERLSLIAVGGYGRGVLAPFS
                     DLDLLFLRPWKSIPRTEQVIEFMLYVLWDLGVKVGQSARSVDECLSLAKSDMVIRTTL
                     LEARPLAGDDRLAEEFIARFRTFVVKADPRPFIAAKLVERDLRHQKSGAVRYRVEPNV
                     KDGKGGLRDLNTLFWIARSLAPESPLGARVLDGLLTARERRTFEESFDFLWRTRAHLH
                     LTAGRAEEKLTFDLQPEVARRMGWRGRGDEPAVERFMRRYFLVARDVGALTRAMSAKL
                     EARQQKPTMSLSRLIPGRKRKLGVDGFTEDAGRLSVKGPEVFAAAPEKLLMLFRTADE
                     HDLDLHPDAFSAVSRSLSLVTPSLRRDPEATRAFLDILAHGQRPYRVLTLMNETGLLG
                     RFLPEWGRIVGQTQFNMYHAYTVDEHTLQAIGIINDIWRGKLKADHPASSEIVHRIDD
                     F"
BASE COUNT          237 a          582 c          497 g          241 t
ORIGIN      
        1 cctgtaccgg ctcaagggtc tgggccgccg gtgacacccg cccccgacga ccggctgaca
       61 gaggccgcgt ccgcccccga cgtgcgcaag gcggtcgcag aaatcctgcg agaaagcttc
      121 gacgcagccc gtgcgaaagc cgcccgccgt ctcgcggccg gcggcgacgg ggtcgaggtg
      181 gcccggctgt cttccgccgc cgccgacgat ctgctgaccg ccctgtggcg cttcaccacc
      241 gaaaccctct accccgccta caatccgacc gaagccgagc ggctcagcct gatcgccgtc
      301 ggcggctacg gccgcggcgt gctggcgccg ttctccgatc tcgacctgct gttcctgcgt
      361 ccgtggaagt cgatcccgcg caccgaacag gtcatcgaat tcatgctcta cgtcctgtgg
      421 gatctgggcg tgaaggtcgg ccagtcggcg cgctcggtcg atgaatgcct gtcgctggcg
      481 aagtccgaca tggtcatccg caccaccctg ctggaggcgc gcccgctggc gggcgacgac
      541 cggctggccg aagaattcat cgcccgcttc cgcaccttcg tggtcaaggc cgacccccgc
      601 cccttcatcg ccgccaaact ggtcgagcgc gacctgcgcc atcagaaatc cggcgccgtc
      661 cgctatcgcg tcgagcccaa cgtcaaggac gggaagggcg ggctgcgcga cctcaacacc
      721 ctgttctgga tcgcccgctc cctggcccct gaaagcccgc ttggcgcccg tgttctcgac
      781 ggcctgctga ccgcgcgcga gcggcggacg ttcgaggaat ccttcgactt tctgtggcgc
      841 acccgggcgc atctgcacct gacagcgggg cgggccgaag agaagctgac cttcgatctc
      901 cagcccgagg tggcgcgccg catgggctgg cgcggacgcg gcgacgagcc ggcggtcgag
      961 cggttcatgc gccgctactt cctcgtcgcc cgcgacgtcg gggccctgac ccgcgccatg
     1021 tcggccaagc tggaggcgcg ccagcagaag ccgaccatga gcctgtcgcg gcttatcccc
     1081 ggccgcaaac gcaagctggg cgtcgacggc ttcaccgagg acgcgggccg cctgtcggtg
     1141 aagggtcccg aggtcttcgc cgccgcgccc gaaaagctgc tgatgctgtt ccgcacggcc
     1201 gacgagcacg atctggacct tcatcccgac gccttctcgg ccgtcagccg gtcgctgtcg
     1261 ctggtcaccc cgtcgttgcg ccgcgacccg gaggcgaccc gcgccttcct cgacattctg
     1321 gcccacggcc agcggcccta ccgcgtcctg accctgatga acgagaccgg cctgctgggc
     1381 cggttcctgc ccgaatgggg gcggatcgtc ggccagaccc agttcaacat gtaccacgcc
     1441 tatacggtcg acgagcacac gctgcaggcc atcggcatca tcaacgacat ctggcgcggc
     1501 aagctgaagg ccgaccatcc ggcctcgagc gagatcgtcc accgcatcga cgatttc
//
LOCUS       DASVVN010000399         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_120518,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000399 DASVVN010000000
VERSION     DASVVN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120518"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VF686_06415"
     CDS             complement(<1..823)
                     /locus_tag="VF686_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEX8661483.1"
                     /translation="MSDATTPRLKVSREGVVLIKSFEGFRPHAVRTDDGRWVIGYGHT
                     ASAREGLSVGEQDAELLLQYDLLPVVKALNERAPASLNQHQFDALASFAISVGVERFL
                     ASDVLQRLNEGQAGRAADALIGWPEEPSPDTRLRRRAAERALFIADPRSAVTLADLLA
                     APLPPPPVEAQAEAPAASDARAAAVAALLGETIEPETSSATPSDAAEPEQGPSAGTVP
                     PPAPTFGSALNRYSPYAATIVGPLPGFEPAAPSEAAVEAAGPAAAPVETEAPSQPA"
     gene            complement(889..>1779)
                     /locus_tag="VF686_06420"
     CDS             complement(889..>1779)
                     /locus_tag="VF686_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8661484.1"
                     /translation="VGDTIDHIRKQGEDLPELFFDIYVVDPVNRPVGGIAISRLLRAP
                     RAAALADLMEPVNEIAVDQDQEEVAYIFEKYHLISAPVIDAEGRLVGQITVDDIVNII
                     QEENREDILRLAGVSDEDRGSSVSEIVRGRVPWLAINLATAVLGASVIAFFGATIEQI
                     VALAVLMPIVSAIGGNAGTQALTVTVRALATRELNSSNAPRTFRRELAVGLANGLVLA
                     PLIGGAAALWSGDWRIGAVIALSMVLNLIVAATVGVLTPLTLSRLKFDPAVSSAVFVT
                     ATTDFFGFLIFLGLATLVLI"
BASE COUNT          302 a          581 c          650 g          246 t
ORIGIN      
        1 atgcgggctg cgaaggcgcc tccgtctcga ccggagcggc ggcgggcccg gcggcttcca
       61 cagccgcttc ggacggcgcc gccggctcga agccgggcag gggaccgacg atggtcgcgg
      121 cgtagggcga atagcggttc agagccgaac cgaaggttgg cgcgggaggc gggacggttc
      181 cggccgaagg gccctgttcc ggctcggcgg cgtcggacgg cgtcgcgctt gaagtctccg
      241 gctcgatcgt ttccccgaga agggcggcga ccgcggcggc gcgcgcatcg gacgcggcgg
      301 gggcttcggc ctgcgcctcg accggcggcg gcggcagggg ggcggccagc agatctgcga
      361 gggtcacggc cgagcgcggg tcggcgatga acagggcgcg ctcggcggcg cggcggcgca
      421 ggcgggtatc cggcgaaggc tcttccggcc agccgatcag ggcgtcggcg gcccgaccgg
      481 cctgaccctc attgagacgc tggaggacgt cggaggcgag gaaccgctcg acccccacgg
      541 aaatggcgaa actggcgagg gcgtcgaact ggtgctggtt caggctggcc ggagcccgtt
      601 cgttcagagc cttcacgacc ggaagcagat cgtactgcag cagcagttcg gcgtcctgct
      661 cgcccacgct cagcccttcg cgggccgagg cggtgtggcc atagccgatg acccagcgtc
      721 cgtcgtcggt ccgcacggcg tgcggccgga agccctcgaa actcttgatg aggacgactc
      781 cctcgcggga gaccttcaga cgtggcgtgg tcgcgtcgga cactgaatga cccaaatcga
      841 gacgggctgg ctcgcccgat gggagaacca gcaaaccgtg ggccgcaatc agatcaacac
      901 aagcgtggcg agaccaagga agatcaggaa tccaaagaag tccgtcgtcg cggtcacgaa
      961 caccgccgag gacacggcag ggtcgaactt cagccgcgac agggtcaggg gcgtcagaac
     1021 ccccaccgtc gccgccacga tcagattcag caccatcgac agggcgatta ccgcgccgat
     1081 ccgccagtcg cctgaccaca gggcagcggc gccgccgatc aggggggcca gcaccagacc
     1141 attggccaga ccgactgcaa gctcgcggcg gaaggtgcgc ggggcgttgg agctgttcag
     1201 ctcgcgcgtc gcgagggcgc gcacggtcac ggtcagggcc tgcgtcccgg cattgccgcc
     1261 gatcgccgag acgataggca tcagcaccgc cagcgcaacg atctgttcga tggtcgcgcc
     1321 gaagaaggcg atgacgctgg cgcccagcac cgccgtggcc agattgatgg ccagccacgg
     1381 cacccggccg cgcacgatct cggacacgga cgagccgcgg tcctcgtcgc tgacgccggc
     1441 gaggcgcagg atgtcctcgc ggttctcttc ctgaatgatg ttgacgatgt catcgacggt
     1501 gatctgaccc accagccgac cttcggcgtc gatgaccggg gccgagatca ggtggtattt
     1561 ctcgaagatg taggcgacct cttcctgatc ctggtcgacg gcgatctcgt tgaccggctc
     1621 catcaggtcc gcgagggcgg cggcgcgagg cgcgcgcagc aggcggctga tggcgatgcc
     1681 gccgaccggc cggttcacgg ggtcgaccac atagatgtcg aagaacagct ccggcaggtc
     1741 ctcgccctgc ttgcggatgt gatcgatggt gtcgccgac
//
LOCUS       DASVVN010000400         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_386192,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000400 DASVVN010000000
VERSION     DASVVN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386192"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VF686_06425"
     CDS             complement(<1..836)
                     /locus_tag="VF686_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEX8661485.1"
                     /translation="MILTCPSCATSYFTPDEAIGPGGRTVRCKDCKHTWRATLDEPLE
                     LSVSTAAAEAASFHSREEAEPESLAETPAPELPRAFRARAEQQRRLRRAATHGVVWAG
                     IASGFAALLASAWLFRVEVVEMAPRAAQAYAAVGLTVNPTGLDFEAITAKPLPSDPGK
                     VLVSGALRNVRDNERTAPPVRVALLDAQGAEIGHAVVRIDAAPVLPGKVQGFAAVIAD
                     PGARGQGIGVDFVLDAPAARPAEPHAPAPVRADTRRAAPPAVHEPSSAPLHEAAAAGL
                     RPA"
     gene            905..1615
                     /locus_tag="VF686_06430"
     CDS             905..1615
                     /locus_tag="VF686_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8661486.1"
                     /translation="MSVQPRRATRSAASTDTVRLSGVGFGYGATHRALRDVNLTLPSG
                     SFHFLTGPSGAGKSTLLKLLTLQARPDAGTLHLFGENATMAPRKALPDFRRRIGVVFQ
                     DFRLLDHLSVFENVALPLRLAGGKQIDYAADVEEMLDWVGLGDRMNDLPPSLSGGEQQ
                     RLAIARAVVAGPDLILADEPTGSVDAKMGERLLKLFQSLNRLGATVLIASHDEALAER
                     SGATILRLDGGRLSGGEA"
BASE COUNT          217 a          586 c          615 g          239 t
ORIGIN      
        1 gcggggcgca ggccggcggc ggcggcctcg tgcagcggcg cggaactggg ttcgtggacg
       61 gcgggcggcg cggcgcggcg ggtgtccgcc cggaccggcg ccggggcgtg cggttcggcg
      121 ggacgggcgg ccggcgcatc aagcacgaag tcgacgccga tgccctggcc gcgggcgccc
      181 ggatcggcga tgacggcggc gaagccctgg accttgccgg gcaggacggg ggcggcgtca
      241 atacggacca cggcgtgtcc gatctcggcg ccctgggcgt ccagcagggc cacgcgcacc
      301 ggcggcgcgg tccgctcatt gtcgcgcaca ttcctgagcg cgcccgaaac cagcaccttg
      361 cccggatcgc tgggcagggg tttggcggtg atggcctcga aatccagacc cgtcgggttc
      421 accgtcagcc cgacggcggc gtaggcctgg gccgcgcgcg gggccatttc gaccacttcg
      481 acgcggaaca gccaggcgct ggccagcagg gcggcgaagc ccgaggcgat gccggcccag
      541 accacgccat gggtcgcggc gcggcgcagg cggcgctgct gttcggcgcg ggcgcggaag
      601 gcgcggggca gttcaggcgc gggcgtctcg gccagtgatt cgggttcggc ctcctcgcgc
      661 gagtggaaac tggcggcctc ggcggcggcg gtcgagaccg acagctccag cggctcgtcc
      721 agcgtggccc gccacgtatg tttgcagtcc ttgcagcgca cggtgcggcc gccgggcccg
      781 atcgcctcat cgggggtgaa atagctggtg gcgcaggacg ggcaggtcag tatcatgtcg
      841 gctgactgcg aggctcttcg ccccctcccg tcgcggttct gaaccgcgtc ctgacccaaa
      901 atcgatgtcc gtccagcccc gccgcgccac acgatccgcc gcctcgaccg ataccgtccg
      961 cctgtccggc gtggggttcg gctatggagc gacgcaccgc gcgctgcggg acgttaacct
     1021 caccttgcca tcgggctctt tccacttcct taccgggccc tccggggcgg ggaagtcgac
     1081 cctgctgaaa ctgctgacgt tgcaggcccg tcccgacgca gggaccctgc acctgttcgg
     1141 cgagaacgcc accatggcgc cgcgcaaggc cctgcccgat ttccgccggc gcatcggcgt
     1201 cgtgttccag gacttccgcc tgctggacca tctgtcggtg ttcgagaacg tggccctgcc
     1261 cctgcgcctc gcgggcggaa agcagatcga ctacgccgcc gacgtcgagg agatgcttga
     1321 ctgggtcggt ctgggcgacc ggatgaacga cctgccgccc tcgctgtcgg gcggcgagca
     1381 gcagcggctg gccatcgccc gcgccgtggt ggcgggcccc gacctgatcc tggccgacga
     1441 accgaccggc agcgtcgatg cgaagatggg cgagcggctg ctcaaactgt tccagtcgct
     1501 gaaccggctg ggcgccaccg tcctgatcgc cagccatgac gaagccttgg ccgagcggtc
     1561 gggggcgacg atcctgcgtc tcgacggcgg ccgattgagc ggaggcgagg cgtgatccgc
     1621 cgcctgttct cccgccgcgc cgccctgctg ccccgcg
//
LOCUS       DASVVN010000401         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_174315,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000401 DASVVN010000000
VERSION     DASVVN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174315"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /locus_tag="VF686_06435"
     CDS             <1..2184
                     /locus_tag="VF686_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase TIM-barrel-like
                     domain-containing protein"
                     /protein_id="HEX8661487.1"
                     /translation="FAGPDDPAYLAAQVAGGEGFDWFYASDADRAAQARTTITDTAHG
                     EHWVFRPKDLKGWWGHAHHDRPGGARSVTPTAWVPGMKPIRLTEFGCAAVDRGGNAPN
                     LFQDPKSAESSLPPFSTGARDDRMQRRALEAVLAHYVGPQNNPVSEVYGGPMLAGADA
                     WCWDARPWPTFPGRGDVWADAGGWRTGHWLNGRLGGDTKDLIAAVLKRGGLAANDFEV
                     GSVSGTVHGYVIDRPMRTRDALEPLLAALGMVAAERNGRVAVIGEATVEAGLMLDALA
                     LPDQGTSVRAERVLEPSPGAARVRFIDGDADYQTGTSVVRSNGAGGGVDLDLPAVCSS
                     SLARAAAQRALEGAADERLTVALGPLETIRLEPGDRVSIEDHVGVWRVMRLNMDETPS
                     AVLERVSSVVAGTDEAGPTTRDEDAGVGAPFFRMIELPPLVRNEADSRPIAVAAAEPW
                     RPMRVFAGASADALTARGDVTRPATVGVLTEPLAAGPRHRWDEVNTLVVRIEGRPPQS
                     RAPVAVLAGANAAAVQTADGWELVQFRTADLVGEDVWRLGGLLRGQQGTEGAMAAGAS
                     VGATVVLLDAEPARADSPPAERGLPLIWRAGPTGQPAGGPFVSEVAHTASGVHDRPWS
                     PAHLRAEGWADGGFNLTWMARLRIDGDRWDGEPAFANPLRFRIRILAGPTALRIFEAE
                     SAWALYSAANAAVDFPGGPGPEAVVAVAQWGEGYGWGTEAMAPLA"
     gene            2309..3247
                     /locus_tag="VF686_06440"
     CDS             2309..3247
                     /locus_tag="VF686_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX8661488.1"
                     /translation="MAGDPYKELGVSRGASADEIKKAFRKLAKELHPDKNPGNTVADE
                     RFKRITAAFDLLGDKEKRAKFDRGEIDADGREQFRGFGGGGQTRGAGGNPFGQGGARQ
                     QGFENIDLDELFGGMFGGGARPGGARSGGFANAGLGRGADVKATLEISLEDSISGATR
                     RIQFSDGRTLDVTIPKGAADGQTIRLRGQGAPGASGRSEAGDALITLKIARHPVFTRD
                     GADLTMDLPVSLPDAVLGGKIPVRTPEGTVSMTIPKGSSSGKVLRLKGRGAFADGKRG
                     DLLAKLMVMLPEEDNAALTKVAQAMRDAGPYKPWRD"
     gene            3247..4398
                     /locus_tag="VF686_06445"
     CDS             3247..4398
                     /locus_tag="VF686_06445"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX8661489.1"
                     /translation="MASRPNVKPARPWFSSGPTAKRPGWSSQALSHELLGRGIRAPEV
                     VERFAHGLRLTRELLQVPADWVLVYVPGSDTAAVEAAMWSMLGERPVQVLAFENFGKV
                     WATDAVDHLRLEPEVLTAPWGGWPDTSKVDPAKDLVFPWNGTTSGVCAPDGDFIGDDR
                     EGVVICDATSAAFAMPLPWSKLDVVTFSFQKALGGEAGLGVAALSPRAVERLDRYEPP
                     RSVPKVLRLRDANGFDRMLANGSMLNTFSVWTIEDWIDAVEWGLAEGGLDALIGRTKA
                     NAAALDAWVLRTDWVEYLAEDKATRSTTSVCLKIVDARVTAMNDDGRRALVTRMKALV
                     EKEGAAFDIESHRNAPAGLRIWCGCTVDVADIEALTPWLDWAFETALSE"
     gene            4481..>5327
                     /locus_tag="VF686_06450"
     CDS             4481..>5327
                     /locus_tag="VF686_06450"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX8661490.1"
                     /translation="MANVAVVGAQWGDEGKGKIVDWLSNRADMVVRFQGGHNAGHTLV
                     VDGKVYKLALLPSGVVQGKPSIIGNGVVVDPWHLVGEIEKIEAQGIRISPDVLIVADN
                     ACLILPIHPALDVAREAAASAPGARIGTTGRGIGPAYEDKVGRRAIRVCDLASAEDLT
                     VKIDRLRAHHDPLRAGLGLEPIDPEALLRQLLEIAPRILPYVQPAWRVLDKARRDGKR
                     VLFEGAQGAFLDVDHGTYPYVTSSNTVAGQAAAGSGIGPRGVGYVLGIVKAYTTRVGE
                     GPFACE"
BASE COUNT          852 a         1576 c         2054 g          845 t
ORIGIN      
        1 ttcgcggggc cggacgatcc ggcctatctg gcggcgcaag tggccggcgg ggaggggttc
       61 gactggttct atgcgtctga tgcggaccgg gccgcgcagg cgcgcaccac gatcaccgac
      121 acggcgcatg gcgagcactg ggtgttccgg cccaaggatc tgaagggctg gtggggccat
      181 gcgcatcatg accggccggg cggcgcgaga agcgtaacgc cgacagcctg ggttccgggt
      241 atgaagccga tccggctgac ggaattcggt tgcgcggccg tggaccgcgg cggcaatgcg
      301 ccgaacctgt tccaggatcc gaagagcgcc gagagcagcc tgccgccgtt ctcgaccggc
      361 gcgcgcgacg accgcatgca gcgccgggcg ctggaggcgg tgctggcgca ctatgtcggg
      421 ccacagaaca atcctgtgtc ggaggtctat ggcggaccaa tgttggcggg cgcggacgcc
      481 tggtgctggg atgcgcggcc ttggcccacg tttccgggac gaggcgatgt gtgggccgac
      541 gccggcggat ggcggaccgg gcattggctg aacggccgac tgggcggcga caccaaagac
      601 ctgatcgcgg cggttttgaa acgaggcgga ctggccgcaa acgatttcga agtcgggtcc
      661 gtaagcggga ccgtgcacgg ctatgtcata gaccggccga tgcggacccg ggacgcgctg
      721 gagccgttgc tggccgcgct gggcatggtc gcggcggaaa ggaacggcag ggttgcggtg
      781 attggagaag cgacggtcga ggccggattg atgctcgacg ccctcgccct gccggatcaa
      841 gggacgagcg tcagggctga gcgggtgttg gagccgtcgc ccggggcggc gcgcgtgagg
      901 ttcatagacg gcgacgccga ctaccagact gggacgtcag tcgttcggtc gaacggcgcg
      961 ggcggcggcg ttgatctgga tttgccggca gtgtgttcgt catcgctggc gcgagccgcg
     1021 gcgcagcggg cgctggaagg tgcagcggac gagcgattga ccgtggcttt gggccccttg
     1081 gaaacgatca ggctggagcc cggcgacagg gtttcgatcg aggaccacgt cggtgtttgg
     1141 cgggtcatgc ggctgaatat ggacgagact ccgtcggcgg tactggagcg ggtgtcatcg
     1201 gtggttgcgg ggactgacga ggctggcccg acgacacgcg acgaggacgc cggcgtcggc
     1261 gcgccgttct tccggatgat cgaactgccg ccgctggtgc ggaacgaggc ggacagccgt
     1321 ccgatcgcgg tcgccgcagc cgaaccgtgg cggcctatgc gggtgtttgc cggggcgtcc
     1381 gcggacgcct tgaccgcgcg gggggacgtc acgcgaccgg cgactgtggg cgtgctgacg
     1441 gagccgctgg cggcgggtcc gaggcatcgc tgggacgagg tgaacacgct ggtggtccgg
     1501 atcgaggggc ggcctccgca gagccgtgca ccggtggcgg tgctcgcggg cgccaacgcc
     1561 gcggcggtcc agacggcgga cggttgggaa ctggtgcagt tccggacagc ggacctggtc
     1621 ggcgaagacg tctggcgcct gggcggcttg ctgcgcggcc agcagggaac cgagggtgcc
     1681 atggctgccg gtgcgtcggt tggggccacc gtggttctgc tggatgcgga accggcgcgg
     1741 gcggactccc cgccggccga gcggggtctg ccgctgatct ggcgagcagg accgacgggc
     1801 caacccgccg gtgggccttt tgtgagcgag gtcgcccata ctgccagcgg cgtacacgac
     1861 cggccctggt cgcccgcaca tttgcgtgcg gagggatggg ccgatggcgg gttcaatctt
     1921 acgtggatgg ctcggttgcg gatcgacggg gaccgctggg acggcgagcc ggcgttcgcg
     1981 aatccactcc ggttccggat cagaattctg gcgggaccaa cagcgctgag gatattcgag
     2041 gccgagagcg cctgggccct gtattccgcg gcgaacgcgg cggtcgattt tcctggcggg
     2101 ccgggccccg aagcggtggt cgcggtggcc caatggggcg agggatatgg ttggggaacc
     2161 gaggcgatgg cgccgctggc ctgactgcga gagagttttc ggcggcgcgg ctttgcggaa
     2221 tccccacgat ggcctaactg atcctttcgg cattcagtgg gctgatgcgc tttgcgctgc
     2281 ttgagcccgg cgcaggagcg atttctaagt ggcgggcgat ccctacaagg aactgggcgt
     2341 ttcgaggggc gcgagcgcgg acgagatcaa gaaggcgttc cgcaagctcg ccaaggaact
     2401 gcatcccgac aaaaaccccg gcaacacggt cgccgacgaa cggttcaagc ggattacggc
     2461 tgcctttgat ctgctgggtg acaaggaaaa gcgcgccaaa ttcgaccgtg gcgagatcga
     2521 cgccgacggc cgtgaacagt tccgcggttt tggtgggggt ggacagacac gcggcgccgg
     2581 cggcaatccg ttcggtcagg gcggcgcgcg acagcagggc ttcgagaaca tcgatctgga
     2641 cgaactgttc ggggggatgt tcgggggcgg agcccggccc ggaggcgcac ggagcggtgg
     2701 cttcgccaac gcgggtctcg gacgcggggc ggacgtcaag gcgacgctgg agatcagcct
     2761 ggaggattcc atctcgggcg cgacccggcg gatccagttc tcggacgggc gaacgctgga
     2821 tgtgactatt cccaagggtg cggccgacgg ccagacgatc cggctgcggg gccagggcgc
     2881 gcccggcgcc tccggccgca gcgaggctgg cgacgccttg attaccctga agatcgcgcg
     2941 gcatccggta ttcacgcggg atggtgcgga tctgacgatg gacctgcccg tgtcgctgcc
     3001 cgacgccgtt ctgggcggca agattccggt gcggactccg gaggggacgg tctcgatgac
     3061 cattcccaag ggatcgagct ccggcaaggt cttgcggctg aaagggcggg gcgcattcgc
     3121 cgacggcaag cggggcgacc tgctggccaa gctgatggtc atgctgcccg aggaggacaa
     3181 tgcggctctg acaaaggtcg cccaagccat gcgggacgcc ggtccctaca agccgtggcg
     3241 cgattaatgg ccagccggcc gaatgtgaaa ccggcgcggc catggttctc gtcgggaccc
     3301 acggcgaagc ggccgggatg gtcgtctcag gcgctctcgc atgagctgct gggccgcggc
     3361 atccgggcgc ccgaggtggt ggagcggttc gcacatggcc tgaggctgac acgcgagctg
     3421 ctgcaggttc ccgcggattg ggtgctggtc tatgtgcccg gatccgacac ggcggcggtc
     3481 gaggccgcca tgtggtcgat gctgggagag cggccggttc aggtgcttgc gttcgagaat
     3541 ttcggcaagg tctgggcgac agacgccgtc gatcatctga gactggaacc cgaggtgctc
     3601 accgcgccat ggggcggatg gcccgacacc tcgaaggtgg atccggcgaa ggacctggtg
     3661 tttccatgga atggcaccac ctcgggcgtc tgcgcgccgg acggggactt catcggcgac
     3721 gatcgcgaag gcgtggtgat ctgcgacgca acgtcggcgg cgttcgccat gcccctgccg
     3781 tggagcaagc tggatgtcgt taccttcagc ttccagaagg cgctcggcgg tgaagcaggc
     3841 ttgggcgtgg cggcgctttc gccgcgggcg gtcgaacggc tggaccggta tgaaccgccg
     3901 cgcagcgtgc ccaaggtgct gcggctgcgc gatgcgaacg gcttcgaccg gatgctcgcc
     3961 aatgggtcga tgctgaacac cttctcggtc tggacgatcg aggactggat cgatgcggtc
     4021 gaatggggac tggccgaggg cggcctggac gccctgatcg ggcggacgaa ggccaatgcg
     4081 gcggcgctgg acgcctgggt gctgcggact gactgggtcg agtatctggc cgaggacaag
     4141 gcgacgcggt ccaccacgtc ggtctgcctg aagatcgtgg atgcccgggt aacggcgatg
     4201 aacgatgacg gccgtcgtgc gctggtcacc cgcatgaagg cgttggtcga gaaagaaggc
     4261 gcagccttcg atatcgagag ccaccgcaac gcgccggccg ggttgagaat ctggtgcgga
     4321 tgcacggtcg atgtcgcgga tatcgaggcg ttgacgcctt ggctggactg ggcgttcgag
     4381 acggcgctgt cggaataatc gtccgacgag aggacacccg ccgattcccc cgctataggc
     4441 tgcgcccgca ttccgaaatc ccggacagcg gagaacagtc ttggcgaacg tcgcggtggt
     4501 cggcgcccag tggggcgacg agggcaaggg caagatcgtc gactggctgt cgaaccgcgc
     4561 cgacatggtc gtcaggttcc agggcggtca caacgccggc catacgctgg tcgtcgacgg
     4621 caaggtctac aagctggcgc tgctgccgtc gggcgtggtg cagggcaagc cctcgatcat
     4681 cggcaacggc gtggtcgtcg atccctggca tctggtcggg gagatcgaga aaatcgaggc
     4741 gcagggcatt cgcatcagtc cggacgtgct gatcgtcgcc gacaacgcct gcctgatcct
     4801 gcccatacat ccggccctgg acgtggcgcg tgaggcggcc gccagtgcgc cgggcgcccg
     4861 gatcggcacg accgggcgcg gcatcggtcc agcctatgag gacaaggtcg gacggcgagc
     4921 gatccgggtc tgcgacctag cgagcgccga ggacctgacg gtcaagatcg accgcctgcg
     4981 cgcgcaccat gatccgctgc gggccggatt gggtctcgaa ccgatcgatc cggaggcgct
     5041 gcttcgccag ctgctggaga ttgcgcccag aatccttccc tatgtccagc cggcatggcg
     5101 ggtgctcgac aaggcgcggc gggacggcaa gcgggtgctg ttcgaggggg cgcagggcgc
     5161 cttcctggac gttgaccacg gcacctatcc ctatgtgacc agctccaaca cagtggccgg
     5221 acaggcggcg gcggggtcgg gcatcgggcc gcgcggggtc ggctatgtgc tgggtatcgt
     5281 caaggcctac acgacccgtg tgggtgaagg ccccttcgcc tgcgaac
//
LOCUS       DASVVN010000402         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_15478, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000402 DASVVN010000000
VERSION     DASVVN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15478"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..627
                     /locus_tag="VF686_06455"
     CDS             205..627
                     /locus_tag="VF686_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661491.1"
                     /translation="MPSWLLFAIVGAVVLLALVALVRFVGLRQRGEGGLDVTGSWRTN
                     YGELSLTQSGRHIAGTYGGNGGLTGEVDGNTVTGYWERSGPTVLCPTARDGTRWWGRF
                     VWTVNETNTAFTGANSACEAEPRPPVNAWSGARVGPPP"
     gene            840..1208
                     /gene="rpsM"
                     /locus_tag="VF686_06460"
     CDS             840..1208
                     /gene="rpsM"
                     /locus_tag="VF686_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX8661492.1"
                     /translation="MARIAGVNIPTGKRVVVALQYIHGVGEKTAKDIMQKVGIDEARR
                     VNELTDQEVLHIREAIDRDHTVEGDLRRETAVNIKRLMDLASYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKPIAGKKK"
     gene            1227..1616
                     /gene="rpsK"
                     /locus_tag="VF686_06465"
     CDS             1227..1616
                     /gene="rpsK"
                     /locus_tag="VF686_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX8661493.1"
                     /translation="MAKEPARVKKRERKNITSGVAHVNASFNNTMITITDAQGNTISW
                     SSAGMMGFKGSRKSTPYAAQRAAEDAGRKAMEHGVRTLEVNVAGPGSGRESALRALQA
                     TGFTITTIRDVTPIPHNGCRPPKRRRV"
BASE COUNT          315 a          666 c          590 g          223 t
ORIGIN      
        1 ctggtcgcgc ggctgccgct cgccgcgcgg gtcgtcgcgc gcccggccag gtccccgccc
       61 agccgatcgt cgcggtaagt tcaccgtccc ctgccacgtc aaccttgacg gcccaagggc
      121 cgaggaggac aacatcaggg agaccccgaa gggccccatg gtcgcttcat ccgtcctcag
      181 tcaggcacgc ctgtgaagcg gaaggtgccg tcctggttgc tgttcgccat cgtgggcgca
      241 gtggtgctgc tggccctcgt cgccctggtc cgcttcgtcg gcctgcgaca gaggggggag
      301 ggcggcctcg acgtcaccgg ttcctggcgc accaactacg gggagctgtc cctgacccag
      361 agcggccgcc acatcgccgg cacctatggc ggcaacggcg ggctgaccgg cgaggtcgac
      421 ggcaatacgg tcaccggcta ctgggagcgc agcggcccga ccgtcctctg ccccacggcc
      481 agggacggca cccgctggtg gggccgcttc gtgtggaccg tcaacgagac caacaccgcc
      541 ttcaccggcg ccaattcggc ctgcgaggcc gagccccggc cgccggtcaa cgcctggtcc
      601 ggcgcccggg tcggcccgcc cccctgaggc cgcgcttgac gcgagccggc gaacgctgcg
      661 atcccctgtc gggcgacccc gggcggccaa aacgtcacgg gcgccgcagg ccgtccgtcg
      721 ccttgacggg caggttttcg tccctataac aggcccttcc gcgaaatgac gcgacagcgg
      781 cgtcggctct ggcgagccgg ggccgtgctt tgcgttcatc gcctgaggag atcccgcgcg
      841 tggcccgtat cgctggcgtc aacatcccaa ccggaaagcg cgtcgtcgtc gcgctccagt
      901 acatccatgg ggtcggtgag aagaccgcca aggacatcat gcagaaggtc ggcatcgacg
      961 aggcccgccg ggtcaacgag ctgaccgacc aggaggtcct gcatatccgc gaggcgatcg
     1021 accgcgacca caccgtggaa ggcgacctcc gccgcgagac cgccgtcaac atcaagcggc
     1081 tgatggacct ggcctcctac cggggcctgc gtcaccgcaa ggggctgccc gtccgcggcc
     1141 agcgcaccca caccaacgcc cggacccgca agggcccggc caagccgatc gccggcaaga
     1201 agaagtaagc ggggcaagag accgacatgg ccaaggagcc ggcccgcgtc aaaaagcgcg
     1261 agcgcaagaa catcaccagc ggcgtcgccc acgtgaacgc cagcttcaac aacaccatga
     1321 tcaccatcac cgacgcccag gggaacacca tctcctggtc cagcgccggg atgatggggt
     1381 tcaaggggtc gcggaagtcg acgccctacg ccgcccagag ggcggccgag gacgccggcc
     1441 ggaaggcgat ggagcacggc gtgcgcaccc tcgaggtgaa cgtcgccggg cccggctcgg
     1501 gccgtgaatc ggcgctccgc gcgctgcagg ccacgggctt caccatcacc accatccgcg
     1561 acgtaacgcc catcccgcac aacggctgcc gtccgcccaa gcggcggcgg gtctagccgc
     1621 ctatatacgc cccgacccga gccggccctg tctcccacat ggccggcgaa ccccgttcag
     1681 gggaccgccc ggactaggac cgcatcgtga tcgagaacgt caatcaaaga aactggcagg
     1741 agctcacccg cccggagaag cccctcatcg agcagggcgc cgatccgcag cgca
//
LOCUS       DASVVN010000403         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_280290,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000403 DASVVN010000000
VERSION     DASVVN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280290"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1768)
                     /gene="leuS"
                     /locus_tag="VF686_06470"
                     /pseudo
     CDS             complement(1..1768)
                     /gene="leuS"
                     /locus_tag="VF686_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
BASE COUNT          284 a          585 c          656 g          320 t
ORIGIN      
        1 cccaggcgcc gtccgcgcga cggtaggtct cgtggatcac catgccttgc gtgaacaggc
       61 cggcgaacgg ctccttgacc gacatcagac cggcgtcgct gagggcccgg ctgatgaagc
      121 gggcgtagag caggtgcagg acggcgtgtt cgaccccgcc gatatactgg tcgaccggca
      181 gccatttgtc ggcggcggct ttcgagatgg gttcggccgc cgtcggatcg gtgaagcggg
      241 cgaaatacca gctggagtcc acgaaggtgt cgagggtgtc ggtttcgcgc acggcctcgc
      301 cgccgcacgt cggacaggac acgtgcttcc aggtcgggtg ccggtcaagc ggattgccgg
      361 gcgtgtcgaa tgtcacgtcc ttgggcagct cgacaggcag ctggtcggca ggcacgccga
      421 cggggccgca tttcgcgcag tggatcacgg ggatgggaca tccccagtag cgctgacggc
      481 tgacgcccca gtcgcgcagg cgatagatgg tcgcgccctg accctgaccg gcggcttcaa
      541 tacgggcgat ggccgcggcc ttggccgctt cgatctccat cccgtccatg aaatcggagc
      601 ggaacaggcg gccggggccg acgtaagctt ctgttccgac tgcgaaatcg tcgccagcgc
      661 cgtcaggtcg cacgaccggg atcacgggca gggcgtattt gcgcgcgaag tcgagatccc
      721 gctggtcgtg ggccgggcag ccgaagatgg ctcccgtccc gtattccgac aggatgaagt
      781 tggcgatcca gaccgacacc tcgcggccgt cgaacggatg gacgacctta agacccgtat
      841 cgaagccgat cttctcggcc tgttcgatct cggcctgggt cgagccgccc ttgcggcatt
      901 cggcgacgaa ggcggcggcg gccgggtcag cctcggcgat ggcgcgggcg atcgggtggt
      961 cgggggccac gcccatgaag ctggcgccga acagggtgtc ggggcgggtg gtgtagattt
     1021 ccagtccgtc cggcgcggcg gcgggcgcgt cgccggccca tttccacgtc atctgcaggc
     1081 ccttggagcg gccgatccag ttctcctgca tcagccggac cttgtcgggc cagcggcctt
     1141 ccagctcctt cagcccctcg atcaggtcgt cggcgtagtc ggtgatgcgc aggaaccact
     1201 ggttcagttt gcgtttctcg accaccgcgc ccgaacgcca gccgcggccg tcgacgacct
     1261 gctcattggc caggacggtg ttgtcgaccg ggtcccagtt gacgacagag tccttgcgat
     1321 agaccaggcc gcgtttgaac agctcgagga accacgcctg ctgcttgccg taataggccg
     1381 ggtcgcaggt ggcgaactcg cgcgaccagt ccagcgacag gcccagcagc ttcagctggt
     1441 cgcgcatggc ggcgatgttg gaccaggtcc agtcaccggg gtggacgccg cgctcgatgg
     1501 cggcgttctc ggccggcagg ccgaaggcgt cccagcccat ggggtgcagg acgtcaaagc
     1561 cctgcgcccg cttcgaccgc gccacgacat cgcccatgac gtagttgcgg gcgtggccca
     1621 tgtggatggc cccggacgga taggggaaca tctccagcac ctagtatttg ggcctgcctg
     1681 ttccctcggt gggggtgcgg aaggcgtcgg cggccgccca gcgggcctgc tggcggggtt
     1741 cggcggtctt gggctcgtat cgggccacgg gattccttca gccgtcggga ttgttgcgat
     1801 gcaccaatcc tagaacaagg gcctgcccga tggggaggtt ttatt
//
LOCUS       DASVVN010000404         5923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_386224,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000404 DASVVN010000000
VERSION     DASVVN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386224"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VF686_06475"
     CDS             <1..133
                     /locus_tag="VF686_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8661494.1"
                     /translation="KEIARRLALSPGTVRNYLSEAASKLGARNRTEAGRIARGQGWL"
     gene            229..1164
                     /locus_tag="VF686_06480"
     CDS             229..1164
                     /locus_tag="VF686_06480"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661495.1"
                     /translation="MTCSRRFVLTAAALGAVIGATGVKAQGLERRPLNQDGLVGEFHF
                     DPAVRSGTAVILLSGSDGGLPSARDARDLASAGFPTLSLAYFKDWNGQPEGVPAGLSE
                     IPLEYFFRAIDWLKSQAQVDPRRIVLAGLSRGAELALLLASLRPDIAGVAAFSPSSVV
                     WRGVPQGAEAASRPAWTLGGQGVPYQIDIPDPTAPIRRGFENAVANPAARIAVEKITG
                     PVLLVSSPADRIWPSDVYANEIAARLATRSPSAPIENLRLEDASHLLMGTGPGVTRFE
                     VPGSSFTVEFGGTPEGTLNGRARAWRALKALLSSL"
     gene            1252..2244
                     /locus_tag="VF686_06485"
     CDS             1252..2244
                     /locus_tag="VF686_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8661496.1"
                     /translation="MAPSYVLRLFDRLLGRRPPADTASDDLKLLVENSRDVLCRLGPD
                     FIIRYCSPSSTAVLGRPPEEMVGQRPSSIVLEEDRPVIAAAAARMVQGEDIKPLILRT
                     LRPDGVMAWVETSSRALRDPQTDALVEIVLVMRDVSERQALQQRLEFEAATDGLTGLA
                     NRRAFDDTLEREWARAARDDTELSLILLDVDHFKAFNDRYGHHAGDACLRDVALAVQE
                     VVRRPGDLAARYGGEEIAVILPGTPASGAAKVADALRLAILDLDVAHADSACGVLTAS
                     VGAATATGAVGLTIAMPAGLLIGADGALYRSKHKGRNRVETVTLLPPKRGAHAA"
     gene            2437..2826
                     /gene="rpsF"
                     /locus_tag="VF686_06490"
     CDS             2437..2826
                     /gene="rpsF"
                     /locus_tag="VF686_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX8661497.1"
                     /translation="MALYEHTVMSRQDISAQQAEALNDTIKGLIEAGGGTVAKIEYWG
                     LRNLTYRVKKNRKAHYSLLAVDCPPAAMAEVERQLSINEDVLRWLTVRVEELDLELSP
                     LLARRERERERERERTPRDDAAAEATF"
     gene            2840..3115
                     /gene="rpsR"
                     /locus_tag="VF686_06495"
     CDS             2840..3115
                     /gene="rpsR"
                     /locus_tag="VF686_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX8661498.1"
                     /translation="MTDTTAPSPGAPAGSGPARRPFYRRRKVCPFTGPQAPKIDYKDV
                     KLLQRYISERGKIVPSRITAVSQIKQRELAKAIKRARYLALLPYVVK"
     gene            3117..3698
                     /gene="rplI"
                     /locus_tag="VF686_06500"
                     /pseudo
     CDS             3117..3698
                     /gene="rplI"
                     /locus_tag="VF686_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
     regulatory      3811..3922
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4201..>5923
                     /locus_tag="VF686_06505"
     CDS             4201..>5923
                     /locus_tag="VF686_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661499.1"
                     /translation="MTRTSFRLALSAAVSASALLAGAAAAQTAPQAPTRDEATVIDEV
                     VVTGTRAPNRSRLDTLSPVDVVTSEDLQNRGTTELGAALAQAVPSLTFQRPAVVDGTD
                     SIRPATLRGLSPDQTLVLVNGTRRHASALVNVNGSVGRGSAAVDLNTIPTGALDRIEV
                     LRDGASAQYGSDAIAGVVNLRLREAASGGGASFTAGQYYTTVDTARTGGRDEEDGGTL
                     TASGWQGFGLGADGFLTLSAEYLQRDPTNRADLDPRVTPNAVTARFGDPEVEQWTVFA
                     NAGLPLGNGWEAYGWAGYQDRDSESAAFPRLANNANNVAAIYPNGFLPKIAINSQDLS
                     LAGGLRGEVGGWAADVNLVYGRNALDFRTEESLNATYGAASPTSFDSGSLIYDQLVLG
                     LDVARQYDIGLYGPLNVAAGLEARRETYEIQAGQPESYNRGPLGGNTALAGGAQGFVG
                     FQPSNEVDEDRTAIGVYLDVEAPVTEQLTVAGAVRFEDYSDFGTSTTGKISARYDFTP
                     AFALRGSVSTGFRAPSLQQSYFTSTASVIQNGAVVETGTFPATSPVAGVLGAEPLDAE
                     TSTNYTLG"
BASE COUNT          953 a         2130 c         1947 g          893 t
ORIGIN      
        1 caaggagatc gcccggcggc tcgccctgtc gccgggcacg gtgaggaatt atctgtccga
       61 agccgcctcg aagctgggcg cgcgcaaccg gacggaggcc ggccgtatcg caagaggcca
      121 gggttggctc tgagcccacc ccggacgaag tcgggatgac atttgtcact gccggagccg
      181 gggcggcggt ctgaaactgc aactcccctg acgaacctgg agtttcggat gacctgttcc
      241 cggcgatttg tgctcaccgc ggccgccctc ggcgccgtca tcggggcgac gggcgtgaag
      301 gcgcagggcc tcgaacggcg gccgctcaat caggacggtc tggtcggtga gtttcatttc
      361 gaccccgccg tccggagcgg aacggccgtc atcctgctgt cggggtccga tgggggcttg
      421 ccgtcggccc gcgacgcccg cgacctggcg tccgcaggct tcccgacgct ttcgctcgcc
      481 tatttcaagg actggaacgg ccagcctgaa ggcgtcccgg ccggcctgag cgaaattccg
      541 ctggagtatt tcttccgggc catcgactgg ctgaagtcgc aggcgcaggt cgatccgcgc
      601 cggatcgtac tggccggcct gtcccgcggc gccgagctgg ccctgctgct cgcgtcgctc
      661 cgtccagaca tcgccggcgt cgccgccttc tctcccagca gcgtcgtctg gcgcggcgtg
      721 ccgcagggcg ccgaggcggc ttcgcgtccg gcatggaccc tgggcggaca gggcgtgccg
      781 taccagatcg acatcccgga ccccaccgcg ccgatccgga gaggattcga gaacgccgtc
      841 gcaaatccgg ccgcgcgcat cgccgtcgag aagatcaccg gcccggtgct gctggtttcc
      901 agcccggcgg acaggatctg gccgtcggat gtctatgcca atgagatcgc cgcgcgtctg
      961 gcgacgcgat cgccgtccgc cccgatcgaa aacctgcggc tcgaggacgc cagccatctg
     1021 ttgatgggga ccggcccggg cgtcacccgg ttcgaagttc cgggctcgtc gttcacggtg
     1081 gagttcggag gcacgcccga gggaaccctg aacggccggg cccgggcatg gcgagccttg
     1141 aaggcgttgc tttcgtcgtt atgacgaccc tccgttcacc atagccgtaa gccacccctt
     1201 aagccggcga gcgcaccctt cgggagttcg ggggatggcc tggaggcggc aatggctccg
     1261 tcgtacgtcc ttcgcctgtt cgatcgcctt ctggggcgga ggccgcctgc cgatacggcg
     1321 tccgacgatc tgaagctgct ggtcgagaac agccgcgacg tcctgtgccg gctggggccc
     1381 gatttcatca tccgctactg ctcgccgtcg tcgacggcgg tgctgggccg cccgccggag
     1441 gagatggtcg gccagcggcc ttcgtccatc gtcctcgagg aagaccggcc cgtcatcgcc
     1501 gccgcggccg cgcgcatggt ccagggcgag gacatcaagc ccctgatcct gcggacgctc
     1561 aggccggacg gggtcatggc ctgggtcgag acgagctcgc gggccctgcg cgatccacag
     1621 accgacgccc tggtcgaaat cgttctggtg atgcgcgacg tatccgagcg gcaggcgctg
     1681 caacagcggc tggagttcga ggccgccacg gacggcctga ccggcctggc caatcggcgg
     1741 gcgttcgacg acacgctcga gcgggagtgg gctcgcgccg cgcgcgacga caccgagctg
     1801 tcgctgatcc tgctggatgt cgaccacttc aaggcgttca acgaccgcta tggccatcat
     1861 gcgggcgacg cctgccttcg cgacgtggct ctggcggtgc aggaggtggt gcgccgcccc
     1921 ggcgatctgg cggcgcgcta cggcggcgag gagatcgccg tcattctgcc ggggaccccg
     1981 gcctcgggcg ccgcgaaggt ggcggacgcg ctgcgtctgg ccatcctcga tctggacgtc
     2041 gcccacgccg acagcgcctg cggcgtcctg accgccagcg tcggcgccgc cacggcgacc
     2101 ggcgcagtgg gcctgaccat cgccatgccc gccggcctgt tgatcggcgc ggacggcgcc
     2161 ctgtaccggt ccaagcacaa gggacggaac cgggtcgaga cggtcaccct gctgccgccg
     2221 aagcgcgggg cgcacgcggc ctgatcgatc gccgcgcaag cgcgccttgc ccgacgggct
     2281 cttttcccct atacgcgccg cctttccagg gcttcggtcc cggctgcgga acaccaaagg
     2341 gttcgggaag tcatcccggc cgccgcgcca ggatccatcg tgggagagga cttacccggt
     2401 cccctcgcgc cgacgcccta caagggagac taccgcatgg ctctttacga gcacacggtc
     2461 atgtcgcgcc aggatatcag cgcgcaacag gccgaagcgc tgaacgacac catcaagggc
     2521 ttgatcgaag ccggcggcgg caccgtcgcc aagatcgagt actggggtct gcgcaacctg
     2581 acctatcggg tcaagaagaa ccgcaaggcg cactattcgc tgctcgccgt cgactgcccg
     2641 cccgccgcca tggccgaagt ggaacgtcag ctgtcgatca acgaggacgt ccttcgctgg
     2701 ctgacggtgc gcgtcgagga actcgacctg gaactgtcgc cgctgctggc ccgccgcgag
     2761 cgcgagcgcg aacgtgaacg cgagcgtacg ccgcgtgacg acgccgccgc cgaagccacc
     2821 ttctaaggga acccggaaca tgaccgacac caccgccccg tcgccgggcg cgccggccgg
     2881 ctccggcccg gcccgccgtc ccttctatcg ccgccgcaag gtgtgcccgt tcacgggccc
     2941 gcaagcgccg aagatcgact acaaggacgt caaactgctg caacgctaca tcagcgaacg
     3001 cggcaagatc gtcccttcgc gcatcacggc cgtttcccag atcaagcagc gcgaactggc
     3061 caaggccatc aagcgcgccc gctatctggc cctcctgccc tatgtggtga agtaagatga
     3121 aggtcgttct gcttgagcgc gtcgataacc tcggcgccat cggcgatgtc gtttccgtca
     3181 aggacggctt cgcccgcaac ttccttctgc cgcgcgacaa ggcccgtcgg gccacgtcgg
     3241 cgaacctgaa ggcgttcgaa ctcgaccgcg ccgccatcga ggcccgcaac gagaagaaca
     3301 aggccgacgc cggcacgatc ggctccaaga tcgacggcca gacctatgtc atgatccggc
     3361 aagccggtga gacgggtcag ctgtacggtt cggttgccgc tcgcgacatc gtcgaggccg
     3421 ttgtggccga aggcggcaag gtcgagcgca gccagatcgt gctcaacacc ccgatcaaga
     3481 ccctcggcgt tcacgagatc accgtgcgtc tgcacgcgga agtctcggcg accgtcaaga
     3541 tcaacatcgc ccgctcggcc gacgaagccg aacgtcaggc gaagggcgag gacgtgatca
     3601 aggccgccta cgacgaggac cgcgaagcgg ccctcgaaca ggccaaggac atggtcgccg
     3661 gcggcgccgg ccagcaggaa ggcctgggtt cggaagccgg aacctctgac ggatgcgaga
     3721 aagtcacggc ggcgcggggg caaccctgcg ccgccgtact tttttccgcc acggacttga
     3781 accgtttatg ccgcatcgca atatcgcatc tgctcatcga gaccggctga gggataggcc
     3841 ctttgacgcc ggggcaacct gcggatttcc gtaaggtgcc aactccttcg ggacccctct
     3901 ttcgggtcgc ggcagatgag aggacgggcc aggcccggac tcccgtcatt tgtcaccgga
     3961 gtggttcccg atcgagcggg agaccaccat gcgtgagcgc acgccctgcc ccgtgactgt
     4021 cctcgccgcc ggcctgcgcg cgcgttgcac cggtccgatg cgatgtcgac gttcgctgta
     4081 accggctgac cgtcccccgg ccctcagacc ggacctgaaa cctcacaatc gcaagaccga
     4141 caccggcgat cgctgcgcgc gaagccgtgc ggaaacctgc gtctcatcga gaacccgaac
     4201 atgacccgaa cctccttccg cctcgccctt tcggcggctg tttccgcctc cgccctgctc
     4261 gccggcgccg cagcggccca gaccgccccg caggccccga cccgcgacga agccaccgtc
     4321 atcgacgagg tcgtcgtcac cggcacccgg gcgccaaacc gctcgcggct ggacacgctg
     4381 tcgccggtcg acgtcgtgac gtccgaagac ttgcagaacc gcggaaccac cgagctgggc
     4441 gccgccctcg cccaggcggt gccgtcgctg accttccagc gacccgccgt ggtcgacggg
     4501 accgactcca tccgccccgc caccctgcgc ggcctgtcgc ccgaccagac gctggtgctg
     4561 gtcaacggaa cgcgtcggca cgcctcggcc ctggtcaacg tcaacggctc ggtgggccgc
     4621 ggctcggccg ccgtagacct gaacaccatt ccgaccggcg cgctggatcg tatcgaggtg
     4681 ctgcgcgacg gcgcctcggc ccagtacggt tcggacgcga tcgccggcgt ggtgaacctg
     4741 cgtctgcgcg aggccgccag cggcggcggc gcgagcttca ccgccggcca atactacacc
     4801 accgtcgaca ccgcccggac cggaggccgc gacgaggagg atggggggac cctgaccgcc
     4861 tcgggctggc agggcttcgg ccttggcgcg gacggcttcc tgacgctgag cgcagaatat
     4921 ctgcagcgcg atccgaccaa ccgcgccgac cttgatccgc gcgtcacccc caacgcggtc
     4981 acggcccgct tcggcgatcc cgaggtcgag cagtggaccg tcttcgccaa cgccggcctg
     5041 ccgctcggca acggctggga ggcctatggc tgggccggct atcaggaccg cgacagcgaa
     5101 agcgccgcct tcccgcgtct ggccaacaac gccaacaacg tcgccgccat ctacccgaac
     5161 ggcttcctgc cgaagatcgc catcaactcg caggacctgt cgctggccgg cggcctgcgc
     5221 ggcgaggtcg gcggatgggc ggccgacgtc aatctggtct acggccgcaa cgccctggat
     5281 ttccgcacgg aggagtccct gaacgccacc tatggcgcgg cgtccccgac cagcttcgac
     5341 tccggctcgc tcatttatga ccagctggtg ctcggcctcg atgtcgcccg ccaatacgac
     5401 atcggcctgt acggcccgct caacgtggcg gcgggccttg aggcgcgccg cgaaacctac
     5461 gagatccagg ccggccagcc ggaatcctac aaccgcggcc ccttgggcgg gaacacggcc
     5521 ctggccggcg gcgcgcaggg cttcgtcggc ttccagccct ccaacgaggt ggacgaagac
     5581 cgcaccgcca tcggcgtcta tctggatgtc gaggcgccgg tgaccgaaca gttgaccgtc
     5641 gcgggagccg tccgcttcga ggactattcc gactttggaa cctcgaccac cggcaagatc
     5701 tcggcccgct acgacttcac cccggccttc gccttgcgcg gctcggtctc gacgggcttc
     5761 agggcgccgt cgctgcagca gtcgtacttc acctcgaccg catcggtgat ccagaacggc
     5821 gcagtggtcg aaaccggcac cttcccggcc accagccccg tcgccggcgt gctgggcgca
     5881 gagccgctgg atgccgagac ctcgaccaac tacaccctcg gcg
//
LOCUS       DASVVN010000405         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_120675,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000405 DASVVN010000000
VERSION     DASVVN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120675"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF686_06510"
     CDS             <1..313
                     /locus_tag="VF686_06510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515721.2"
                     /note="Catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8661500.1"
                     /translation="EDQLKAEGVAYKAGKFPFTANSRAKINHETDGFVKVLADAATDR
                     ILGVHILGPQAGEMIGEACVAMAFGGSSEDVARICHPHPTRSEAVKQAAMGVEGWTMQ
                     A"
     gene            complement(333..1316)
                     /locus_tag="VF686_06515"
     CDS             complement(333..1316)
                     /locus_tag="VF686_06515"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX8661501.1"
                     /translation="MTGLDRRVLLAGLGALGLPAGPAAAQDVLEPLVEARPIQLLSNL
                     FTRVGAAVTVNGHGPFTFVIDTGAGTTSIADTLADRLQLSAQQSVIVHGITEARVTRS
                     VAINRLLLSGLGFRNLTCPVFPRDQLGADGLIGLDVLDRFRLKFNVARRTASLNVRGL
                     SVIMGGDLVTGSRLRREGMRSVRGRFGQMIMTQVSVDGQPTAAFIDSGAQYSIGNMAL
                     RQAAAARRRDGGRVARQVPLYGVTGQSLSADLAMVDDLRFGPQRLGPTPMLFADLHCF
                     DTLELSGRPALLIGADLLGRFREVTLDFPGNLVVFEGLRRQTTSRLEIPGV"
     gene            complement(1400..>2034)
                     /locus_tag="VF686_06520"
     CDS             complement(1400..>2034)
                     /locus_tag="VF686_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX8661502.1"
                     /translation="RRCGTPSPQLALVRGPRIKASLPRPVSEDQARGMMQEPDADPDA
                     EPWEAARDRAVLTLLYGCGLRISEGLSLTRADAPVPETLRIIGKGGKTRLAPVLPAVR
                     TAVDAYLALQPFPLEPGDALFRARRGGPLTPRHVQAMVQRLRGRLGLPASATPHALRH
                     SFATHLLGAGADLRAIQELLGHASLSTTQKYTAVDAKHLLEAYSAAHPRR"
BASE COUNT          324 a          699 c          734 g          277 t
ORIGIN      
        1 ggaggaccag ctgaaggccg agggcgtcgc ctacaaggcc gggaaattcc ccttcaccgc
       61 caacagccgc gccaagatca accacgagac cgacggcttc gtgaaggtgc tggccgacgc
      121 cgccaccgac cggattctgg gcgtccacat cctcggcccg caggccgggg agatgatcgg
      181 cgaggcctgc gtagccatgg ccttcggcgg atcgtcggaa gacgtcgccc gcatctgcca
      241 ccctcacccg acccgttcgg aggccgtcaa acaggcggcc atgggcgtcg aaggctggac
      301 gatgcaggcc tgatcgatcg ggctccgccc cgtcagacgc cggggatctc gagccggctg
      361 gtcgtctgac ggcgcaggcc ctcgaacacg accagattgc ccgggaaatc cagggtgacc
      421 tcgcggaacc ggcccagcag gtcggcgccg atcagcaggg cgggccggcc ggacagttcc
      481 agggtgtcga aacagtgcag gtccgcgaac agcatgggcg tcgggccgag ccgctggggc
      541 ccgaaccgca ggtcgtcgac catggccaga tcggcgctca gactctggcc cgttacgccg
      601 tagagcggca cctggcgggc cacgcggccg ccgtccctgc gccgcgccgc ggcggcctgc
      661 cgcaacgcca tattgccgat cgaatactgg gccccgctgt cgatgaaggc cgccgtcggc
      721 tgaccgtcca cgctgacctg ggtcatgatc atctggccga agcggccgcg gaccgaacgc
      781 atgccctcgc ggcgcaggcg ggagcctgtc accaggtctc cccccatgat gaccgagagg
      841 ccgcgcacat tcaggctggc cgtgcggcgc gcgacgttga acttcagccg gaagcgatcc
      901 agcacgtcca ggccgatcag gccgtccgcg cccagctggt cgcgcgggaa gaccgggcag
      961 gtgaggttgc ggaaccccag tccgctgagc agcagccggt tgatggccac gctgcgcgtc
     1021 acccgggcct cggtgatccc gtgaacgatg acggactgct gcgccgacag ctgcagccgg
     1081 tccgccaggg tgtcggcgat ggaggtcgta cccgcgcccg tatcgatcac gaaggtgaag
     1141 gggccgtgcc cattgacggt gaccgccgcg ccgacgcggg tgaacagatt gctcagcagc
     1201 tggatgggcc gagcctcgac cagcggttcc agtacatcct gtgcggcggc gggtccggca
     1261 ggcaggccca aagcccccag tccggccaac aggacgcgtc ggtccaggcc tgtcatgcag
     1321 ttcacgctcc gcgccgcctc cccagcggat ggaccgtcac tgtaggctgg cttgacggac
     1381 cctgaccagt ccgtcgtttt cagcgccgcg gatgagcagc ggaatatgct tccaacaggt
     1441 gttttgcgtc gacggcggtg tatttctggg tcgtcgacag gctggcgtga cccagcagtt
     1501 cctgaatggc ccgcaggtcc gccccggcgc ccagcaggtg ggtggcgaag ctgtgtcgca
     1561 gggcgtgggg cgtggccgag gcgggcaggc ccagacggcc gcggagcctt tgcaccatgg
     1621 cctggacatg gcgaggcgtc agaggaccgc cgcgcctggc ccggaacagg gcgtcgcccg
     1681 gttccagcgg aaagggctgc agcgccagat acgcgtccac ggccgtgcgc acggccggca
     1741 ggaccggggc cagccgggtc ttgccgccct tgccgatgat gcgcagcgtc tcgggcacgg
     1801 gcgcatcggc gcgggtcagg gacagaccct cggaaatgcg aaggccgcag ccgtacagca
     1861 gggtcaggac ggcgcggtcg cgcgcggcct cccacggttc ggcgtcggga tcggcgtccg
     1921 gctcctgcat catgccgcgg gcctgatcct cgctgacggg ccgcggcagg gaggccttga
     1981 tcctggggcc acgcaccagc gccagctgcg gggacggggt gccgcagcgg cgat
//
LOCUS       DASVVN010000406         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_40976, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000406 DASVVN010000000
VERSION     DASVVN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40976"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2353)
                     /gene="uvrA"
                     /locus_tag="VF686_06525"
     CDS             complement(<1..>2353)
                     /gene="uvrA"
                     /locus_tag="VF686_06525"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8661503.1"
                     /translation="DVDLIEGLSPAISIEQKTTSRNPRSTVGTVTEIHDYMRLLWARV
                     GVPYSPATGLPIESQTISMMVDKLTALPDGERLMLLAPVVRGRKGEYRKEMAEWQRAG
                     FQRLKIDGEYYAIEDAPTLDKKFKHDIDIVVDRIVTKEGLEGRYADSLQTALGLADGI
                     AVAEWANVAEGEKEPRRLTFSEKFACPVSGFTISEIEPRLFSFNNPFGACPACDGLGV
                     KLAFDADLVIPDRDKTLHKGAVAPWSRGPSPLYTQTLQSLSRHYGFSMDVAWRELPEK
                     AQEVILRGSGGDKIKFTYDDNARKYEVSKAFEGVLPNLERRWRETDSSWVREELGRFQ
                     SETPCEVCHGKRLKPEALAVKVAGTDIAEVSWLSIKHAHEWFTALNDKLTDKQMEIGR
                     RVLKEITDRLRFLNNVGLDYLSLSRASGTLSGGESQRIRLASQIGSGLTGVLYVLDEP
                     SIGLHQRDNSRLLESLRGLRDLGNSVLVVEHDEEAILTADYVIDMGPAAGIHGGEVCA
                     QGTPAEVMANPKSLTAKYLTGEREIELPPEGRRPVDRKKMLKISGATGNNLKNVTGEI
                     PAGLFTCVTGVSGGGKSTFTIETLYKAAARRLHNASDAPAPFDRIEGLEMFDKVIDID
                     QSPIGRTPRSNPATYTGAFGPIRDWYAGLPESKARGYGPGRFSFNVKGGRCEACQGDG
                     LIKIEMHFLPDVYVTCDVCKGKRYNRETLEIVFKGKSISDVLDMTVEEAASFFKAVPT
                     IRDKMETLKRVGLGYIHVGQSATTLSGGEAQRVKLSKELSKRAT"
BASE COUNT          355 a          736 c          818 g          444 t
ORIGIN      
        1 cgtcgcccgt tttgagagct ctttcgagag cttgacgcgc tgcgcctccc cgccggacag
       61 ggttgtcgcg gactggccga cgtggatata gccgaggccg acccgcttga gggtctccat
      121 cttgtcgcgg atcgtcggca cggccttgaa gaagctcgcc gcttcctcga ccgtcatgtc
      181 cagaacgtcg gatatgctct tgcccttgaa gacgatttcc agcgtctcgc ggttgtaacg
      241 cttgcccttg cagacgtcgc aggtgacgta gacgtcgggc aggaaatgca tctcgatctt
      301 gatcaggccg tcgccctggc aggcctcgca gcggccgccc ttgacgttga acgagaagcg
      361 gccggggccg tagccgcgag ccttggactc cggcaggccg gcgtaccagt cgcgaatggg
      421 gccgaaggcg ccggtgtagg tggccgggtt cgagcgcggg gtgcggccga tgggcgactg
      481 gtcgatgtcg atgaccttgt cgaacatctc cagcccctcg atccggtcga agggggccgg
      541 ggcgtccgag gcgttgtgca gccggcgggc ggcggccttg tagagggtct cgatggtgaa
      601 ggtcgacttg ccgccgccgg acacgccggt gacgcaggtg aagaggcccg cgggaatctc
      661 gccggtgacg ttcttcagat tgttgccggt cgcgccgctg atcttcagca tcttcttgcg
      721 gtcgacggga cggcggcctt ccggcggcag ttcgatctcg cgctcgccag tcaggtattt
      781 ggcggtcagc gacttcggat tggccatcac ctcggccggc gtgccctggg cgcagacctc
      841 gccgccgtgg atgccggcgg ccggacccat gtcgatgacg tagtcggcgg tcaggatcgc
      901 ctcctcgtca tgttcgacga cgaggacgga gttgccgagg tcgcgcaggc cgcgcaggct
      961 ttccagcagg cgggagttgt cgcgctggtg caggccgatg gacggttcgt ccagcacata
     1021 gaggacgccc gtaaggcccg agccgatctg cgacgccaga cggatgcgct ggctctcgcc
     1081 gccggacagg gtgcccgagg cgcgcgacag gctgaggtag tcgaggccga cgttgttgag
     1141 gaagcgcagc cggtcggtga tctccttaag gacgcggcgg ccgatctcca tctgcttgtc
     1201 ggtcagcttg tcgttcaggg cggtgaacca ttcgtgggcg tgtttgatcg acagccagga
     1261 cacctcggcg atgtccgtgc cggcgacctt gaccgccaag gcctcgggct tgagccgttt
     1321 gccgtggcag acctcgcaag gggtctcgga ctggaaccgg cccagctcct ctcggaccca
     1381 tgaggaatcc gtctcgcgcc agcgccgttc gaggttcggc agcacgccct cgaaggcctt
     1441 ggagacctcg tatttgcggg cgttgtcgtc gtaggtgaat ttgatcttgt cgccgcccga
     1501 gccgcgcagg atcacctcct gggccttttc gggcagttcg cgccacgcca catccatcga
     1561 gaatccgtag tggcggctca gggactgcag ggtctgggtg tagaggggcg aggggccgcg
     1621 cgaccacggc gcgacagcgc ccttgtgcag ggtcttgtcg cggtcgggga tgaccagatc
     1681 ggcgtcgaag gccagcttga ctcccagacc gtcgcaggcc gggcaggcgc cgaacgggtt
     1741 gttgaacgag aacagtcgcg gctcgatctc ggagatcgtg aaaccggaca ccggacaggc
     1801 gaatttctcc gagaaggtca gccggcgcgg ctccttctcg ccctccgcga cgttggccca
     1861 ttcggccacg gcgatgccgt ctgccagacc gagggcggtc tgcaggctgt cggcgtagcg
     1921 gccctcaagg ccttccttgg tgacgatccg atccacgacg atgtcgatgt cgtgcttgaa
     1981 cttcttgtcc agcgtcgggg cgtcctcgat ggcgtagtat tcgccgtcga tcttcaggcg
     2041 ctggaagccg gcgcgctgcc actcggccat ttccttgcgg tattcgccct tgcggccacg
     2101 caccacgggc gccagcagca tcagccgctc gccatcgggc aaggcggtca gcttgtcgac
     2161 catcatggag atggtctggc tctcgatcgg caggccggtc gcgggtgaat agggcacgcc
     2221 cacgcgcgcc cacagcaggc gcatatagtc gtggatttcg gtgacggtgc ccacggtcga
     2281 ccgcgggttt cgactggtgg tcttctgctc gatcgaaatc gccggcgaca atccctcgat
     2341 cagatcgaca tcg
//
LOCUS       DASVVN010000407         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_251575,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000407 DASVVN010000000
VERSION     DASVVN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251575"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF686_06530"
     CDS             <1..383
                     /locus_tag="VF686_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8661504.1"
                     /translation="NIRFNPNHTGDDLIAWLNAMAGEAQAESGLRVELEHLCSGNAFL
                     TEPGPFVEGVQDAVQAATGRRPEASTTGGTSDARFIRAMCPVLELGLVGQTMHQIDER
                     APVAEIEALTEVYRRVIGTWFQRA"
     gene            complement(380..517)
                     /locus_tag="VF686_06535"
     CDS             complement(380..517)
                     /locus_tag="VF686_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661505.1"
                     /translation="MRELPVQMPPMRLQRRGGPLREIFYFLVAFGAMFGAALLGLDLL
                     V"
     gene            complement(590..1912)
                     /locus_tag="VF686_06540"
     CDS             complement(590..1912)
                     /locus_tag="VF686_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8661506.1"
                     /translation="MTIRRLFAASSLALVLSAGLAHAQTAPIALERVEAAVAAETPKV
                     VAWRRDIHANPELGFAETRTAALVAEHLRALGMEVRTGVGKTGVVGVLRGGRPGRTVG
                     LRTDMDALPVLEATGLPFASTATGTYQGNTVPVAHACGHDAHIAMLMGAAEVLAGMKA
                     DIAGTVVFIFQPAEEGAPPGEPKGGAALMIEEGALENPRPDAIFGLHVVPGRPGTVFY
                     RPQGFMAASDRIDIVLHGRQTHGAWPWKGVDVIAVSAQIIQTINTLTARTIDPTTTPT
                     VFTIATIDAGVRYNIIPEEATLSGTLRTFDIAQRDDLVRRAGVAIDNVAETFGATAEF
                     SVRQNAALVYNNEELSAWLAPVLTEAAGAGNVNAAAPPTTVAEDFSYLSQVVPGVFYH
                     LGGSKDGVDPTASPPNHSPGFDVNESILPLGVKTHVLSALRFLERPTP"
     gene            1942..2349
                     /locus_tag="VF686_06545"
     CDS             1942..2349
                     /locus_tag="VF686_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661507.1"
                     /translation="MRKAAALAQGRAAGDLDSMAIKIAKTRLRDVVLVCRKCSKRLDG
                     KGFGPDGDRTLKKALRKYLKAGKGKKAELVVKETDCFDICPKNAVIAVNAARPKALLI
                     VRPGADLFEVKAKLGLDDGRRLKPSLRLVSDED"
     gene            complement(2353..>2679)
                     /locus_tag="VF686_06550"
     CDS             complement(2353..>2679)
                     /locus_tag="VF686_06550"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8661508.1"
                     /translation="HTRLVPGFVVDTVMEDGARRVTFGNGMTVREVVVDIDDARRRFA
                     YSVRAEGFEHHNASNEVIGDGEGQCRFIWIADFLPDDLEDTVGQMMGQGADILKATME
                     KAAAEV"
BASE COUNT          433 a          918 c          928 g          400 t
ORIGIN      
        1 tgaacatccg cttcaacccc aaccacaccg gcgacgacct gattgcctgg ctgaacgcca
       61 tggcgggcga ggcacaggcc gaaagcggcc tgcgcgtcga gctggagcac ctgtgctcgg
      121 gcaacgcctt cctcaccgag cccggcccct tcgtcgaagg cgtgcaggac gcggttcagg
      181 ccgcgaccgg acggcgaccg gaggcctcga ccaccggcgg cacctcggac gcccgcttca
      241 tccgcgccat gtgcccggtg ctggagctag gcctcgtcgg ccagaccatg caccagatcg
      301 acgaacgcgc cccggtggcc gagatcgagg cgctgaccga ggtctatcgc cgcgtgatcg
      361 ggacgtggtt ccagcgggcc tagaccagca ggtccaatcc cagcagcgcc gcgccgaaca
      421 tcgccccgaa ggcgaccaga aagtagaaga tctcgcgcag cggcccgccg cgccgctgaa
      481 gccgcatcgg cggcatctgg actggcaatt ccctcaccac accctcctcg aaggacgccc
      541 gaggatgcgc cggcgtcctt gccggcgcgt ttagaaacgt ggttgccgat cagggagttg
      601 ggcgttccag gaaccgcagc gcgctgagca catgggtctt cacgcccagc ggcaggatcg
      661 attcattgac gtcgaagccg ggcgaatggt tcggcggcga ggcggtcgga tcgaccccgt
      721 ccttcgaccc gccgaggtga taaaagacgc cggggaccac ctgcgacaga tagctgaagt
      781 cctcggccac ggtggtcggc ggagcggccg cgttgacgtt gcccgcaccc gccgcctcgg
      841 tcagcactgg agccagccag gccgacagtt cctcgttgtt gtagaccagc gccgcgttct
      901 gacggaccga gaattcggcc gtcgcgccga aggtctccgc gacattgtcg atggccaccc
      961 cggcccgccg caccagatcg tcgcgctggg cgatgtcgaa ggtccgcagc gtgcccgaca
     1021 gcgtcgcctc ttccgggatg atgttgtagc gcaccccggc gtcgatggtg gcgatggtga
     1081 agaccgtcgg cgtcgtggtc gggtcgatgg tgcgggccgt gagggtgttg atcgtctgga
     1141 tgatctgggc cgagacggcg atgacgtcga cgcccttcca cggccaggcg ccgtgcgtct
     1201 ggcggccgtg caggacgatg tcgatccggt cagacgcggc catgaagccc tgcggccggt
     1261 agaagaccgt gccgggccgt ccgggcacca catgcaggcc gaagatggcg tcgggccggg
     1321 gattctccag cgccccctcc tcgatcatca gggccgcgcc gcccttgggc tctcccggag
     1381 gcgcgccttc ctcggcgggc tggaagatga agacgacggt tccggcgatg tcggccttca
     1441 tgcccgccag cacctcggcg gcgcccatca gcatggcgat gtgggcgtcg tgaccgcagg
     1501 catgggccac cggcaccgta ttgccctgat aggttcccgt ggcggtcgag gcgaacggca
     1561 ggcccgtcgc ctccagcacc ggcagggcgt ccatgtcggt gcgcaggccc acggtccggc
     1621 ccggccgccc gccgcgcagc acgccgacca cgccggtctt gcccacgccc gtgcggacct
     1681 ccatccccag cgcgcgcaga tgctcggcca ccaaggccgc cgtgcgggtc tcggcgaagc
     1741 ccaattcggg gttcgcgtgg atgtcgcgtc gccaggccac gactttcggc gtttcggccg
     1801 ccaccgccgc ctcgacccgc tccagcgcga tcggcgcggt ctgggcatgc gcgagccctg
     1861 cggacagaac cagcgccagg ctggaggcgg cgaacaggcg gcggatggtc atgggcgtcc
     1921 ccgatggcgt gagggcgcaa ggtgcgcaaa gccgccgccc ttgcgcaagg gcgcgcggcg
     1981 ggcgatctgg actccatggc catcaagata gcgaagaccc ggttgcgcga cgtcgtgctg
     2041 gtctgccgca aatgctcgaa acggctggac ggcaagggct tcggccctga cggcgacagg
     2101 acgctgaaga aggcgctgcg caaatatctg aaggccggaa aaggcaagaa ggccgagctg
     2161 gtggtcaagg agaccgactg tttcgacatc tgtccgaaga acgcggtgat cgcggtcaac
     2221 gcggcccggc ccaaggcgct gctgatcgtg aggcccggcg ccgatctgtt cgaggtcaag
     2281 gcgaagctgg gcctcgacga cggtcgccgc ttgaagccct cgctcaggct ggtcagcgac
     2341 gaggactgaa cgtcagactt cggcggccgc cttctccatc gtcgccttca ggatgtccgc
     2401 gccctgtccc atcatctggc cgacggtgtc ctctaggtcg tccggcagga agtccgcgat
     2461 ccagatgaac cggcactgtc cctcgccgtc gccgatgacc tcgttcgacg cattgtggtg
     2521 ttcaaaaccc tcggcgcgga cgctgtaggc gaagcggcgg cgggcgtcgt cgatgtcgac
     2581 caccacctcg cgcacggtca tgccgttgcc gaaggtgacc cggcgggcgc cgtcctccat
     2641 cacggtgtcg acgacgaagc cgggcacgag gcgggtgtg
//
LOCUS       DASVVN010000408         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_462879,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000408 DASVVN010000000
VERSION     DASVVN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462879"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF686_06555"
     CDS             complement(<1..392)
                     /locus_tag="VF686_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661509.1"
                     /translation="MKTLVSISVATAALLIAGGASAQIAGGIGGQVGGSVGVGIPQTA
                     PITQGVGSIARDGVNTTRDTVRETRETVRDARPDVDVNANANVRADARANRDGAAVDA
                     AIQTGAMVRSSDGATLGSVVDVTRNTAGR"
     gene            519..1295
                     /locus_tag="VF686_06560"
     CDS             519..1295
                     /locus_tag="VF686_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX8661510.1"
                     /translation="MKIRVRADLVYRFDPPTDAIYKIQVAHWPGQTVLEESLTTTPQI
                     DFIQDEDEEFGARTLRAHLSGEVALVYEAVVDNGVLKGLPPVTVQHDWNDLPAEVLTY
                     LNPSRYCPSDQFGRFAERTFGGTVGGDRVLKILEWIKAEIDYEPGSSDSETTAARTFI
                     DRAGVCRDFTHMGITLCRASGIPARAVSAFAHQLTPPDFHAIFEVWLSNGWWLVDPTG
                     LAPVEGLVRIACGRDAADIAFLTTQGSCQLVRQSVTAEAV"
     gene            complement(1292..>1524)
                     /locus_tag="VF686_06565"
     CDS             complement(1292..>1524)
                     /locus_tag="VF686_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEX8661511.1"
                     /translation="TRDGDHLVLEWVTGDGGVFPMPVEIEIDGRRQTLAMTGGRGRIA
                     APGSAHVLIDPENKVLRRLDFIEAWQAAGSPG"
BASE COUNT          224 a          499 c          550 g          251 t
ORIGIN      
        1 cggccagccg tattgcgggt cacgtccacg accgagccga gcgtggcccc gtcggaactg
       61 cggaccatgg cgccggtctg gatggcggcg tcgaccgcgg cgccgtcgcg gttggcgcgg
      121 gcgtcggcgc ggacattggc gttggcgttc acgtcgacgt ccggacgcgc atcgcggacg
      181 gtctcacgcg tctcgcggac ggtgtcgcgg gtggtgttga cgccgtcgcg ggcgatggag
      241 ccgacgccct gggtgatcgg ggcggtctgg ggaatgccga cgccgacaga accgccgacc
      301 tgaccgccga tgccgccggc gatctgggcg gaagcgccgc ccgcgatcag cagggcggcg
      361 gtggctacgg agatcgatac aagggttttc atgatactcg tccttggaat caggtgcagc
      421 cccctgcgcg tcgagaacga ttggctggta tgggggttcc tgttcaggga gccggttcgc
      481 cgtcccctct ttcatcccga caacgcttca gagcccccat gaaaatccgt gtccgcgccg
      541 atctggtcta ccgtttcgac ccgccgacgg acgccatcta taaaattcag gtcgcccact
      601 ggccgggcca gacggtgctt gaggagagcc tgacgacgac gccgcagatc gacttcatcc
      661 aggacgagga cgaggagttc ggggcccgca ccctgcgcgc ccacctgtcg ggcgaggtgg
      721 cgctggtcta tgaggcggtg gtcgacaatg gcgtgctcaa ggggctgccg ccggtgactg
      781 tgcagcacga ctggaatgac ctgccggccg aggtcctgac ctatctgaac cccagccgct
      841 actgcccctc ggaccagttc ggacggttcg ccgagcgcac cttcggcggc acggtgggcg
      901 gcgaccgggt gctgaagatt ctcgaatgga tcaaggccga gatcgactat gagccggggt
      961 cttcggacag cgagacgacg gcggcgcgga ccttcatcga ccgggccggg gtgtgccgcg
     1021 acttcaccca tatgggcatc accctgtgcc gggcctcggg catacccgcg cgcgcggtca
     1081 gcgctttcgc gcaccagctg accccgccgg acttccacgc catcttcgag gtctggctgt
     1141 cgaacggctg gtggctggtc gatccgacgg ggctggcgcc ggtcgagggt ctggtgcgca
     1201 tcgcctgcgg gcgcgacgcg gccgacatcg ccttcctgac cacccagggg tcgtgtcagc
     1261 tggtgcggca gtcggtgacg gcggaagcgg tctagccggg ggatcccgcc gcctgccagg
     1321 cctcgatgaa gtcgaggcgg cgcaggacct tgttctccgg gtcgatcagg acgtgggcgc
     1381 ttccaggcgc ggcgatgcgg ccgcggccac cggtcatggc cagcgtctgg cggcggccat
     1441 cgatctcgat ctcgaccggc atggggaaga cgccgccgtc gccggtgacc cattccagaa
     1501 ccagatgatc gccgtcgcgg gtct
//
LOCUS       DASVVN010000409         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_147645,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000409 DASVVN010000000
VERSION     DASVVN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147645"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF686_06570"
     CDS             <1..663
                     /locus_tag="VF686_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX8661512.1"
                     /translation="DLVSARSDKTSRALTLGLSGDSRDGLGGGGRNSFSAGWAFGDLH
                     LKSPADRAADALTARTEGGFSKLQISAARLQTVSGPLSLYAAVRGQLAFGNLDSSEKT
                     ELGGAYGVRAYPEGEAYGDEGYLATLEARLMLNRWTGRLPGELQLFGFADFGQVKFAD
                     DPWFAGSNSASRSGIGAGLSWAAPGGALVRLSYARKLGDAVATSGPDKDGRFWFQTSK
                     LF"
     gene            713..>3128
                     /locus_tag="VF686_06575"
     CDS             713..>3128
                     /locus_tag="VF686_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX8661513.1"
                     /translation="MNGSNQSPRASALFGSTALVRLAGLALLGWAAALPNGAAAYAQS
                     VPTGGSVSSGSASIATGSNSVTVSQSSQNVAINWQSFSIDKGSAVVFVQPNSTSVALN
                     RVTGADASVILGSLTANGKVFLVNPNGILFGKGSNVNVGGLVASSLNMSDADFMAGRY
                     TFSGTGGAVLNQGTIVADGGFVALLGANVSNEGTIQANLGTVALAAGEAITLDVAGDG
                     LLNVAIDKGAVNALVNNGGLLRANGGQVLMTAQAAGQLLRTAVNNSGIIEARTLENRN
                     GVIKLLGDMESGTMSVAGVLDASAPDGGDGGFIETSAATVTIADDVMVTTAAPLGRTG
                     TWLIDPQDFTIAATGGNISGATLSKQLVTSSVVISTIVGPGAALPGEGHIHVDDAVAW
                     TSSGPSGTATTLTLNAVGDVNINAAITATKGNLVVCCGGDVNVKAPITTTNGSVLLNA
                     GQNVNVFHAITTTDGNIALCAGHDVHIDAKVTLTRGSTIAAQSLGLPVGLTLIAGADG
                     TGPGAGGGTVVFAPLAPPITVTKAPVSIAYNPVSYAAPTDFSTKFVLTEGAALTQRML
                     LFPHADKVADGTTGAVLSGFNTTAASGEPTGVTLVAGPAASAVFDSAAAGSNIGIAYS
                     GYSLAGANADRYALAGSCCVSTFRTSGAITAAASPPPPAPPPPAPPPPAPPPPAPPPP
                     APPPPAPPPPAPPPPVPPPPAPPPPTPPPPAPPPPAPPPPPVPPPPPPVVVVTPPVVT
                     PPLVTAPPLAGPAPPPVVPPVLIASPILTPLLPAWPGGPQVAVVDGGVRMPAIEAARN
                     DVPDEED"
BASE COUNT          482 a         1175 c         1025 g          446 t
ORIGIN      
        1 gacctggtct cggccaggtc cgacaagacc agccgggctc tgaccctcgg cctgagcggc
       61 gattcccgcg acggcctggg cggcggcggg cggaacagct tctcggccgg ctgggcgttc
      121 ggcgacctcc acctgaagag ccccgccgac cgcgccgccg atgcgctgac ggcgcggacc
      181 gagggcgggt tcagcaagct ccagatcagc gcggcccgac tgcagacggt cagcggccct
      241 ctctcgctct acgccgcggt gcgcgggcag ctggcgttcg gcaatctcga cagctccgag
      301 aagacggagc tcggcggcgc ctatggagtc cgcgcctatc ccgagggcga ggcctatggc
      361 gacgaaggct atctggcgac gctcgaggct cggctgatgc tcaaccggtg gacgggcagg
      421 ctgcccggcg agctccagct tttcggattt gccgacttcg gccaggtcaa attcgccgac
      481 gatccctggt tcgccggatc gaacagcgcc agccgaagcg ggatcggggc aggcctgagc
      541 tgggccgctc cgggaggcgc cctcgtccgg ctgtcctacg cccgcaagct gggcgacgcc
      601 gtggcgacct ccggccccga caaggacggc cgcttctggt tccagacttc gaagctgttc
      661 tgatgcccgg acagcaaaat acgcggaagc caattcaccg aaggaccatc ccatgaacgg
      721 cagcaatcaa tcgccccgag cgagcgccct gttcggatcg accgctctgg tgcgcctcgc
      781 cggcctggcg ctgctgggct gggcggccgc ccttcccaac ggggcggcgg cttatgcgca
      841 aagcgttccc acgggcggat ccgtctccag cggcagcgcc agcatcgcca ccggctccaa
      901 ctcggtcacg gtcagccaat cgagccagaa cgtcgcgata aactggcagt ccttttcgat
      961 cgacaagggc agcgccgtcg tcttcgtgca gccgaacagc acgtcggtcg cactcaaccg
     1021 ggtgacgggg gcagacgctt cggtgatcct cggaagcctc acggccaacg gcaaggtctt
     1081 cctcgtcaac ccgaacggga tcctgttcgg caagggctcc aacgtcaacg tgggcggcct
     1141 cgtcgcctcg agcctgaaca tgtccgatgc ggacttcatg gccggccgct atacattttc
     1201 cggaacgggc ggcgccgtgc tcaaccaggg cacgatcgtc gcggacggcg gcttcgtcgc
     1261 actgctcggc gccaatgtca gcaacgaggg gacgatccag gccaatctcg gcaccgtcgc
     1321 cctggcggcg ggcgaggcga tcactctcga cgtcgccggc gacgggctgc tcaacgtcgc
     1381 catcgacaag ggggcggtga acgctctggt gaacaatggc ggcctgcttc gcgccaatgg
     1441 cggccaggtg ctgatgacgg cccaggcggc cggccagctg ctcaggaccg cggtcaacaa
     1501 cagcggaatc atcgaagccc ggaccctgga gaaccgcaac ggagtcatca agctgctggg
     1561 cgacatggag agcggcacga tgagcgtcgc cggagtgctc gacgccagcg ctccggacgg
     1621 cggcgacggc ggcttcatcg aaacctccgc ggcgacggtg accatagccg acgacgtcat
     1681 ggtcaccacc gccgccccgc tcggccggac cggcacatgg ctgatcgacc cgcaggactt
     1741 caccatcgcg gcgaccggcg ggaacatctc gggcgccacc ctctcgaaac agctggtgac
     1801 cagcagcgtc gtcatcagca cgatcgtcgg gccgggcgcc gctcttccgg gcgaaggcca
     1861 tatccatgtc gacgacgcgg tcgcctggac ctcctcgggg ccctcgggga ccgcgacgac
     1921 cttgaccctg aacgcggtcg gcgacgtcaa catcaatgcc gcgatcaccg ccaccaaggg
     1981 caatctggtc gtgtgctgcg ggggcgacgt gaacgtcaag gcgccgatca cgaccaccaa
     2041 cggaagcgtc ctgctcaatg ccgggcagaa cgtgaacgtc ttccacgcga tcaccaccac
     2101 cgacggcaac atcgcccttt gcgccggcca tgacgtccac atcgacgcca aggtcacgtt
     2161 gacccggggc agcacgatcg ccgctcaaag cctcggcctg ccggtcgggc tgaccctgat
     2221 cgcgggcgcc gacggcacgg gcccgggcgc gggcggcggt acggtggtct tcgcgccgct
     2281 tgcgccgccc atcacggtca cgaaagcgcc cgtctcgatc gcctacaatc cggtctccta
     2341 tgcggcgccg accgacttct cgaccaagtt cgtcctcacc gagggtgcgg cgctgaccca
     2401 gcgcatgctg ctcttcccgc acgccgataa agtggcggac gggactaccg gcgccgtcct
     2461 gagcgggttc aacacgaccg cggcctccgg cgaaccgacc ggggtgacgc tggtcgccgg
     2521 accagccgct tcggccgttt tcgacagcgc cgccgcgggg tcgaatatcg ggatcgccta
     2581 ttccggctac agcctggccg gggcgaatgc ggaccgatac gcgcttgccg gatcgtgctg
     2641 cgtgtcgacg ttcaggacca gcggggcgat tactgccgcc gcttcgccgc cgcccccggc
     2701 gccaccccct cccgcgcctc cgcctcccgc gcccccgccg cccgcgccgc cgccgcctgc
     2761 accgccgccc cctgcgccgc cacctccggc gccgccgccg cccgtgccgc cacctccggc
     2821 gccaccgccg cccacgcctc ctcctccggc gccgccgccg cctgcgccac cgccgcctcc
     2881 tgtgccgccg cctccgccgc cggtcgtggt cgtgactccg ccggtggtaa cgccgccttt
     2941 ggtcacagcc cctcccctgg ccggtcccgc gccgccgccg gtagtgccgc ccgtcctcat
     3001 cgcctctccg atcctgaccc cgctgctgcc ggcctggccc ggtggcccgc aggtcgcggt
     3061 ggtggacggg ggcgtcagga tgccggcgat cgaggccgcc cggaacgatg tccccgacga
     3121 ggaggaca
//
LOCUS       DASVVN010000410         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_251690,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000410 DASVVN010000000
VERSION     DASVVN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251690"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="VF686_06580"
     CDS             complement(<1..1945)
                     /locus_tag="VF686_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661514.1"
                     /translation="MTKTILLATTALLLTAGAASAQTPSEQQAAPQADASQQGVLIFE
                     PAFFADQRPNSALDMVNRVPGFAVVDGDGSRGFEGAVGNILINGSRPASKNDTGSAVL
                     SRTLAGQVERIELIRGGAPGIDMQGFAVVVNVITKAESSRQHIVTLNANIVAVNQDIW
                     GGSYQFTAKEGETTWGVTLSDGISMSDANGVGPVIRRNGSGAIIRQETYTNDQYGGGT
                     SARGNFATPLYGGKIDLTARYGVNDYYSINLQTSPTVRRENLFEEDGSGGEFGAVYTR
                     PLSTRLNIESRFIHQFNDFENLSTSRTRDNGVDSPEQRFSSDGKSSETIARALARFEK
                     SPALTFEGGGELAYNMLDIEQAFTVGGVAVPLPSASVMVEELRGELFGKATWRVSPTL
                     TVEGGLRVEASTITQSGDAEQEKSFFFPKPRILATWTPMPNNQFRFRFERELGQLDFG
                     DFAASSDLDDENVLGGNADLEPEQRWITEATYERRFWTDGIISIGLRHDEIVDAIDVI
                     PLDGGLSAVGNIGDGTLDQLAVNILVPMDRFGFTGGRLGFRNTWNHTEVTDPTTGETR
                     AISGLRPSQPVFTIAQDINSWKLQWGGAYIATLHQFSYDPDQTSGFRGADYFELWAEY
                     KPTATLSIRAQINVWDDFEMQRTV"
BASE COUNT          320 a          612 c          728 g          388 t
ORIGIN      
        1 agaccgtgcg ctgcatctcg aaatcgtccc agacgttgat ctgggcgcga atcgacaggg
       61 tcgcggtcgg cttgtactcg gcccagagct cgaaatagtc cgcgccgcgg aagcccgagg
      121 tctggtcggg atcgtagctg aactggtgca gggtggcgat ataggcgccg ccccactgca
      181 gtttccagga gttgatgtcc tgggcgatgg tgaagaccgg ctgcgaaggc cgaagtcccg
      241 agatggcgcg cgtctcgccg gtggtcgggt cggtcacttc ggtgtggttc cacgtgttgc
      301 ggaagccgag ccgcccgccc gtgaagccga acctgtccat cggcaccagg atgttgaccg
      361 ccagctgatc cagcgtgccg tcgccgatgt tgccgaccgc cgacaggccg ccgtccagtg
      421 ggatgacgtc aatggcgtcg acgatctcgt cgtggcgcag gccgatgctg atgatgccgt
      481 cggtccagaa acggcgctca taggtggctt cggtgatcca gcgctgttcg ggttccagat
      541 cggcgttgcc gcccaggacg ttctcgtcgt ccagatccga ggatgcggcg aagtcgccga
      601 agtccagctg gccgagttcg cgttcgaagc ggaaccggaa ctggttgttc ggcatcggag
      661 tccaggtcgc caggatgcgg ggtttgggga agaagaagct cttctcctgc tcggcgtcgc
      721 cggactgggt gatggtcgag gcttccacgc gcaggccgcc ttcgacggtc agcgtcgggc
      781 tgacacgcca tgtcgccttg ccgaagagtt ccccccgcag ctcctcgacc atcacggagg
      841 ccgaaggcag gggcaccgcg acgccgccca cagtgaaggc ctgctcgatg tccagcatat
      901 tgtaggcgag ctctccgccg ccctcgaagg tcagcgcggg cgacttttcg aaacgggcga
      961 gggcgcgggc gatcgtctcg gacgatttgc cgtcgctgct gaatcgttgc tccggactgt
     1021 cgacgccgtt gtcgcgggtg cgggaggtcg agaggttctc gaaatcgttg aactggtgaa
     1081 tgaaccggct ctcgatattg aggcgcgtgc tcagggggcg ggtgtagacc gccccgaatt
     1141 cgccgcccga cccatcctcc tcgaacaggt tttcccgccg caccgtgggc gaggtctgca
     1201 ggttgatgct gtagtagtca ttgacgccgt agcgggcggt caggtcgatc ttgccgccgt
     1261 acagcggcgt ggcgaagttg ccgcgggccg aggtgccgcc gccgtactgg tcgttggtat
     1321 aggtttcctg ccggatgatg gcgccgctgc cattgcggcg gatgaccggg cccacgccgt
     1381 tggcgtcgct catggagatg ccgtcgctga gggtcacgcc ccaggtcgtt tcgccctcct
     1441 tggcggtgaa ctggtaggag ccgccccaga tgtcctgatt gacggctacg atgttggcgt
     1501 tgagcgtgac gatatgctgg cggctggatt ccgccttggt gatcacgttc acgacgacgg
     1561 cgaagccctg catgtcgatg ccgggagcgc cgccccggat caactcgatg cgctcgacct
     1621 ggccggccag ggtgcgcgac aggacggccg agccggtgtc gttcttggat gcaggccgcg
     1681 agccgttgat caggatgttg ccgaccgcgc cctcgaaccc gcggctgccg tcgccgtcga
     1741 cgacggcgaa gcccggaacc cggttgacca tgtccagcgc ggagttgggg cgctggtcgg
     1801 cgaagaaggc gggctcgaat atgaggacgc cctgttgcga ggcgtccgcc tggggagcgg
     1861 cctgctgttc actgggcgtc tgggcggagg cggcgcccgc ggtcagcaag agcgccgtcg
     1921 tcgccagcag gatggtcttg gtcattgcag gattcccctc gtcatgtgac gtgagatgtg
     1981 gctgtgtgtc gcggctcgct ccagttacaa gacagacagg tggattaaat cggagacagg
     2041 attacagc
//
LOCUS       DASVVN010000411         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_41213, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000411 DASVVN010000000
VERSION     DASVVN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41213"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF686_06585"
     CDS             <1..508
                     /locus_tag="VF686_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8661515.1"
                     /translation="AAAAKSELARFARGSIASLETPAALERAPAYVFRTRNGAEARLA
                     DFRGKVVVVNVWAMWCLPCRTEMPTLARLAEAYEGRDLVVLPINVDNDADRIADAKSF
                     IDVHEPLPLYSDPKFQLPFELPGKGKMPQTVLLDRQGRIRAHFSGEADWASPEVRALI
                     DALLAEGR"
     gene            complement(545..835)
                     /locus_tag="VF686_06590"
     CDS             complement(545..835)
                     /locus_tag="VF686_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661516.1"
                     /translation="MRIQTVTLAALAALSLAACSQAEQDNAQADANEAAADTGQAAGE
                     AGSALKEEAAQVGSAIAAGAGEVAQEVDEATDTLAEKADDQKAETATDTKGN"
     gene            complement(899..1534)
                     /gene="msrA"
                     /locus_tag="VF686_06595"
     CDS             complement(899..1534)
                     /gene="msrA"
                     /locus_tag="VF686_06595"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8661517.1"
                     /translation="MSRRALACLILLAIAACSPGEPAESAPSASASQARDSGRREVAV
                     FAGGCFWSTEASFERMPGVVSAVSGFAGGAGPAPTYEQVVRGGTGHLEAVQVTFDPAR
                     ITYRQLVDRFWRTIDPTDPDGQFCDQGPSYATAVFATAAQKPAADASRRAAAARIGAA
                     RFVTPVRDAARFWPAEAYHQDFARLHPERYAGYTRFCGRAARLRAVWGEAA"
BASE COUNT          247 a          584 c          662 g          261 t
ORIGIN      
        1 ggccgccgcc gcgaaaagcg aactggcgcg cttcgcccgc ggctcgatcg cgtcgctgga
       61 gaccccggcc gcgctggagc gagcgccggc ctatgtgttc aggacacgca acggcgccga
      121 ggcccgactg gcggatttcc ggggcaaggt ggtggtggtc aacgtatggg ccatgtggtg
      181 cctgccctgc cgtactgaaa tgccgaccct ggcccgtctg gccgaggcct atgaaggccg
      241 ggatctcgtc gtcctgccca taaatgtcga caacgacgcc gacaggatcg ccgacgcgaa
      301 gagcttcatc gacgtgcacg agccgttgcc gctgtacagc gacccgaaat tccagctgcc
      361 gttcgaactg cccggcaagg gcaagatgcc ccagacggtg ctgctggacc ggcagggccg
      421 gatccgcgcg catttctcgg gcgaggccga ctgggccagc cccgaagtcc gcgccctgat
      481 cgacgccttg ctggctgagg ggcgctaacg gccgatacgc ggtcgcggcc ttgagcgctg
      541 ggcgtcagtt gcccttggtg tcggtggcgg tctccgcctt ctggtcgtcg gccttttccg
      601 cgagggtgtc ggtggcctcg tcgacctcct gcgcgacctc gccggcgccg gcggcgatgg
      661 ccgaaccgac ctgggccgct tcttccttca gcgcggagcc ggcttccccg gccgcctgac
      721 ccgtgtcggc ggcggcctcg ttggcgtcgg cctgggcgtt gtcctgttcg gcctggctgc
      781 aggcggcgag ggacagggcg gcgagggcgg cgagggtgac ggtctggatg cgcatgggaa
      841 atcccccttc agcgtggaac cttgacggta aacgcggcaa ggaggcgccg gttgcgcttc
      901 acgccgcctc gccccagacc gcccgcagac gcgccgcccg gccgcagaac cgggtatagc
      961 ccgcatagcg ctcggggtgc agccgggcga aatcctggtg ataggcctcc gcaggccaga
     1021 aacgggcggc gtcgcgcacc ggcgtgacga agcgggcggc cccgatccgc gcggcggcgg
     1081 cgcggcgcga ggcgtcggcg gcgggcttct gggctgcggt ggcgaagacg gcggtggcgt
     1141 aggacgggcc ctggtcgcag aactggccgt cgggatccgt cgggtcgatg gtccgccaga
     1201 accggtcgac cagctgtcga taggtgatgc gggcgggatc gaaggtgacc tggaccgcct
     1261 ccagatgtcc ggttccgccg cgcaccacct gctcataggt cggggccggc ccggcgcccc
     1321 cggcgaagcc ggatacggcc gagacgaccc ccggcatgcg ttcgaaactg gcctcggtcg
     1381 accagaagca gccgcccgcg aagacggcga cctcgcgccg gccgctgtcg cgggcctggg
     1441 acgcggacgc cgagggtgcg ctttccgcgg gctcgccggg cgagcaggcg gcgatggcga
     1501 gcaggatcag acaggcgagg gcgcggcgcg acatgggacg gtctccggcg gacgcgtcgg
     1561 gcgtccgcct gaacatacga tccctcgcgg cgcgaggttt caaggcctct caaagcaaaa
     1621 accgccggcc ctctttcgag gaccggcggc tgatgttgtc ttgcctggcg tccttcggct
     1681 tgagggccga aggggggccg atcggccttc cggggttagc ggaagttgat gccgatagtg
     1741 gcgcgacggt tgag
//
LOCUS       DASVVN010000412         6459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_386515,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000412 DASVVN010000000
VERSION     DASVVN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386515"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF686_06600"
     CDS             <1..886
                     /locus_tag="VF686_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661518.1"
                     /translation="GLAAGACSGNGILLDISDPVKPVRLDAAADKNFAYWHSATFNDA
                     GTKVVFTDEWGGGIGARCRAEDPSTWGANMVATIENRTLRGRAFHKLPSAQGADENCV
                     AHNGMIIPVPGRDLMVQAWYQGGVSVMDFTDPTKPVEIAAFDRGPIEDGRLFVAGSWS
                     AYWLNGRIYSSEIARGLDVLKLVPGEHLSAAEIAAAEAVRSDQINPQTQTRISWADSP
                     DVAQSYLDQLMRAPAADAVLTAQVQAGIDQWRRSARNRSGAAEIAAALSAAAEKETGT
                     IAKGRLTALAGLFTRRAG"
     gene            991..1974
                     /locus_tag="VF686_06605"
     CDS             991..1974
                     /locus_tag="VF686_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661519.1"
                     /translation="MYKFVPITSALVLALAAPTAVLADPGNGKGGDKQERGNRGGDDH
                     GRGNDGRGNGDRGNRGNGNGNGRGGDDRRDVRVAVTPDGRAIAVVRDDDRGRGNGNGR
                     GGDDIREVRREVARQVFQTRDGRPVVYFSRQDDRGLIAGCPPGLAKKNNGCLPPGQAR
                     QIARAAEAARYDWLWRTGDDAFNYRYDDGYLYRTDRQGGLLGWLPVLGGALSPGAVWP
                     AQYQYQPAPDYYSSYFGLPQNQQYRYADGALYGVDPQTQMISQVAALLTGQNIGVGQP
                     LPAGYDVYNVPYAYRNQYADGPNAMYRYNDGYVYQADPKTQLVTAVIQLLT"
     gene            2000..2554
                     /locus_tag="VF686_06610"
     CDS             2000..2554
                     /locus_tag="VF686_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8661520.1"
                     /translation="MKTMMRWTLAPLAVMALAACGAKEESGEAQAAATPSNRTITATL
                     KDDRSFGTLERVLDNAALGDVLAGKGPYTVFAPSDAAFTASAGDLGDEAMKAQGAAML
                     RAHIVPGALTRADIVAAMERGGSGTVEMRTMANSLLTFSKEGEAIVVTGDNGARARLT
                     GDESLASNGVVQPVDALLVKPAAG"
     gene            complement(2551..3258)
                     /locus_tag="VF686_06615"
     CDS             complement(2551..3258)
                     /locus_tag="VF686_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661521.1"
                     /translation="MKILAPEPEPRAVVVTLTRPPPPPPPPPAPVIAEPPPGPAQAAP
                     AAAPSPAPPRPVPARAAPRPRPVRTPPPPDVPTLPANPTPVVAAMAVIGEAQLAGALK
                     AGTGQGAGSGAGGGGGSGGANGGSCDMVGRLQDALRADPEVRDAVTRAHRAMPASGRA
                     ILVWDGDWIQDPGQSGKGLAGVRQAIALEVAFAPRECRTQTMRGLAVIAFAEGPAAPK
                     LALGEASWRWSDLLGAR"
     gene            3700..4242
                     /locus_tag="VF686_06620"
     CDS             3700..4242
                     /locus_tag="VF686_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8661522.1"
                     /translation="MTGVVLALGLAASGCASHKFVRENLAVVDERVTTVDTRLTEVDG
                     TARQALDRATAAHKLAEGKFLYEVVLSDDSVKFPVNRDALSPEAEQRLAELAQRLKGE
                     NRNVYLEIQGYTDASGDAEYNEQLGAARAEAVRRSLSRQGIALNRMATISYGEEAPVA
                     PNETREGRAQNRRVAIVVLS"
     gene            4383..5135
                     /locus_tag="VF686_06625"
     CDS             4383..5135
                     /locus_tag="VF686_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX8661523.1"
                     /translation="MTDAQPLVFTDNPMNPLEEALYEGLADASQMGLFERLMLEADLY
                     AAPEPDSPGGTPGDDGNKVLRQGEQLILRGVVLDDGRNSVTLFTDPRRATKMFGENSR
                     IIAMQGRALLDILKDAVVLLNPEGGKGLLLNPDQIKAVLGHEPIRPLRPVSGRVELHD
                     IEPENEPVMLTLAIQKSFKGAMVKDVWLARALWPDVGQIGWFLDMRSDGPADEIKALV
                     VRSVRGLKFGTETLDVSVGKPGGKPGVGMKII"
     gene            complement(5132..6256)
                     /locus_tag="VF686_06630"
     CDS             complement(5132..6256)
                     /locus_tag="VF686_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX8661524.1"
                     /translation="MQTTAAVLRTMGAARPYADSRPLSIETVTLDAPGQGEVMVAIKA
                     AGLCHSDLSVINGDRPRPLPIALGHEAAGVVEALGDGVTDLAVGDHVVMVFMPSCGHC
                     NPCAEGRPALCEPGAAANGKGELLTGGRRIHDDAGDLNHHLGCSAFASHAVVSRRSLV
                     KVDADLPFEHAALFGCAVLTGVGAVVNTAGVRAGQSVVVVGLGGVGLSSVLGAVAAGA
                     SPVVAVDLSEDKLALARTLGAVQTVNAADADAVEQVRALTSGGADFVFEMAGSVRALD
                     AAWRMTCRGGTTVTAGLPPPEAALAVNVVALVAEERTLKGSYIGTCVPGRDIPRYVAL
                     FRQGRLPVDRLLTGVVPLSDINAAFDRLADGQAVRTVVRF"
BASE COUNT         1019 a         2285 c         2319 g          836 t
ORIGIN      
        1 cggccttgcg gcgggcgcgt gctcgggcaa cggcatcctt ctggatatct ccgatcccgt
       61 gaagccggtt cgactcgatg cggcggccga caagaacttc gcctactggc actcggccac
      121 cttcaacgac gccggcacca aggtcgtctt caccgacgag tggggcggcg gcatcggcgc
      181 gcgctgccgg gctgaggatc cgtcgacctg gggcgccaac atggtcgcca ccatcgagaa
      241 ccgcaccctg cgcggccgcg ccttccacaa gctgccttcg gcccaggggg ccgacgagaa
      301 ttgcgtcgcc cacaacggca tgatcatccc cgtccccggc cgcgacctga tggtccaggc
      361 ctggtatcag ggcggcgtct cggtcatgga cttcacggac ccgacgaagc ccgtcgagat
      421 cgccgccttc gaccgcggcc cgatcgagga cggcaggctg ttcgtcgccg gctcgtggtc
      481 ggcctactgg ctcaacggcc ggatctactc cagcgagatc gcccgcggcc tcgacgtgct
      541 gaaactggtc ccgggcgagc atctgtcggc ggccgagatc gcggcggccg aggccgtccg
      601 ctccgaccag atcaatccgc agacccagac gcggatcagc tgggccgaca gccccgacgt
      661 ggcccagtcc tatctcgacc agctgatgcg cgcccccgcc gccgacgccg tcctgaccgc
      721 acaggttcag gccggcatcg accagtggcg ccggtcggcc cgcaaccgca gcggcgccgc
      781 cgagatcgcc gccgccctga gcgccgcggc ggagaaggag acgggcacga tcgccaaggg
      841 ccggctgacg gcgctggcgg ggctgttcac gcgacgggcg gggtaggtac ggcggctccg
      901 cgtccctgag ggcgcggggc ctgcctgggc tttcttcccg gaacacccgg cgaaggcagg
      961 cgttgtgtcg ggaacccgat agaggctccc atgtacaaat tcgtcccgat cacctccgcc
     1021 ctcgtcctcg ccctcgcggc gccgacggcg gttctcgccg accccggcaa cggcaagggg
     1081 ggcgacaaac aggaacgcgg aaaccgcggc ggcgacgatc acggccgcgg taacgacggg
     1141 cgcggcaacg gcgatcgtgg aaaccgcggc aacgggaacg gcaatggacg gggcggcgac
     1201 gaccgtcggg acgtccgcgt cgccgtcacc cccgacggcc gcgccatcgc cgtggtccgc
     1261 gacgacgacc gcggccgcgg caacgggaat ggccgcggcg gggacgacat ccgcgaggtc
     1321 cgccgcgagg tcgcgcgtca ggtgttccag acccgcgacg ggcgccccgt ggtctatttc
     1381 agccgtcagg acgaccgcgg cctgatcgcc ggttgcccgc ccggactggc gaagaagaac
     1441 aacggctgcc tgccgcccgg tcaggcgcgc cagatcgccc gcgccgcgga ggccgcccgc
     1501 tatgactggc tgtggcggac cggcgacgac gccttcaact accgctacga cgacggctat
     1561 ctgtaccgga cggaccggca gggcggtctg ctgggctggc tgcccgtgct gggcggggcc
     1621 ctgtcgcccg gggccgtctg gccggcgcag tatcagtacc agccggcgcc cgactactat
     1681 tccagctatt tcggcctgcc gcagaaccag cagtaccgct acgccgacgg cgccctgtac
     1741 ggcgtcgatc cgcagaccca gatgatcagc caggtcgcgg ccctgctgac cggccagaac
     1801 atcggcgtcg gccagcctct gcctgcgggc tacgacgtct acaatgtgcc gtacgcctac
     1861 cggaaccagt atgcggacgg gccgaacgcc atgtaccgct acaacgacgg ctatgtgtac
     1921 caggccgatc ccaagaccca gctggtgacg gccgtcatcc agctgctgac ctgatcccga
     1981 ccgcgacaga ggcatgacca tgaagacgat gatgcgctgg acgctggccc ctctcgccgt
     2041 gatggccctg gccgcctgcg gcgcgaagga agagtccggc gaggcgcagg ccgccgccac
     2101 gccctcgaac aggacgatca cggcgacgct gaaggacgac cggtcgttcg gcaccctcga
     2161 gcgggtgctg gacaacgccg ccctcggcga cgtgctggcg ggcaaggggc cctatacggt
     2221 gttcgcgccg tcggacgcgg ccttcaccgc ctcggcgggc gacctcggcg acgaggcgat
     2281 gaaagcgcag ggggcggcca tgctgcgggc ccatatcgtg cccggggcgc tgacccgcgc
     2341 cgacatcgtc gccgccatgg agcggggcgg ctcgggcacg gtcgagatgc gcaccatggc
     2401 caacagcctg ctgaccttct cgaaggaggg cgaggccatc gtggtcaccg gcgacaacgg
     2461 cgcccgcgcg cgcctgaccg gcgacgagag cctggcctcc aacggcgtcg tccagccggt
     2521 cgacgccctg ctggtcaagc cggccgccgg ctagcgcgcc cccagcaggt cggaccagcg
     2581 ccagctggcc tcgcccagcg ccagtttcgg ggccgccggc ccttccgcga aggcgatgac
     2641 ggccagaccg cgcatggtct gggtccggca ttcgcgcggg gcgaaggcga cttccagggc
     2701 gatggcctga cgcacgccgg ccagaccctt gccggactgt ccgggatcct ggatccagtc
     2761 gccgtcccag accaggatgg cgcggcccga ggcgggcatg gcgcggtggg cgcgggttac
     2821 ggcgtcccgc acctcggggt cggcccgcag ggcgtcctgc agccgcccga ccatgtcgca
     2881 cgacccgccg tttgcgccgc ccgagccgcc gcctccgccc gcgcctgagc cggcgccttg
     2941 accggtcccg gccttgagcg cgccggccag ctgggcctcg ccgatgacgg ccatggcggc
     3001 gaccacgggg gtgggattgg ccggcagggt cgggacgtcc ggcggcggcg gcgtgcggac
     3061 cggccgggga cgcggcgcgg ccctggcggg aaccggccgg ggcggagcgg gcgagggggc
     3121 cgcggccggc gcagcctgcg ccggtcccgg cggcggctcg gcgatgaccg gcgcgggcgg
     3181 aggcggcggt gggggcggcg gacgggtcag ggtgacgacg acggcgcgcg gttcgggctc
     3241 gggcgcgaga attttcatag gcggcgaggg cgaggaaacg agaaccacaa acaggaccag
     3301 atgcgccgac aggctgagcg cggcggccgg cgtccagacc cgcagccagc gccgcgaccg
     3361 cgccggacgg cgcgccggcg ggaggcctcc cgaagaggga ttttccgttt tcgtcccgga
     3421 gcttggctgc acgttggcgt cccgaccggc gggcccgcct gcgcccgcgt cgtcctgaaa
     3481 gaccagccgt ccgcgtcaac aacgtggcgc ccgggcggca tctgcagacg ggctccatac
     3541 ggctcagcta atggtcaagg tgacgtcttt cggccttatg cgggtcatca ctccgccccg
     3601 cggattacgc caggttcaga cccgcgcacc tatgcgggag ccgcgcaccc gccaatcagg
     3661 agacgacact tgaccagatc gaaactcagg acggcgacca tgaccggcgt ggttctcgcc
     3721 cttggcctcg cggccagcgg ctgcgccagc cacaaattcg tgcgcgagaa tctggccgtg
     3781 gtcgacgagc gggtgaccac ggtggatacc cggctgaccg aggtcgacgg cacggcccgc
     3841 caggcgctgg accgcgccac cgcggcccac aaactggccg aaggaaagtt cctctacgag
     3901 gtggtgctgt ccgacgactc ggtgaaattc cccgtcaacc gcgacgccct ctcgcccgag
     3961 gccgaacagc gtctggccga actggcccag cgtctgaagg gcgagaaccg caacgtctat
     4021 ctggaaatcc agggctatac ggacgccagc ggcgacgccg aatacaacga gcagctgggc
     4081 gccgcccggg ccgaggccgt gcgccgctcg ctgagccgcc agggcatcgc cctgaaccgg
     4141 atggcgacca tctcctatgg cgaggaagcg ccggtggccc cgaacgaaac gcgggaaggc
     4201 cgcgcccaga accggcgcgt cgccatcgtc gtcctcagct aggacgaccg ctcctgcggg
     4261 cggccgggcc ttcgggaccg gccgcctgtc cgggtgctgg atggacgcgg gcgattgacg
     4321 ccgcgattga cggggcgcgt ccgctcgcct cttgtgtccg ccctttcgtt ccagagttga
     4381 ccatgaccga cgcccagccc ctcgtcttca ccgacaatcc gatgaacccg ctcgaggagg
     4441 ccctgtacga ggggttggcc gatgcgtcgc agatgggtct gttcgagcgg ctgatgctcg
     4501 aagccgacct gtacgcggcg cccgagcccg atagtccggg cggcacgccg ggcgacgacg
     4561 ggaacaaggt cctgcgtcag ggcgagcagt tgatcctgcg cggtgtcgtg ctggacgacg
     4621 gccgcaacag cgtgaccctg ttcaccgatc cgcgccgggc cacgaagatg ttcggcgaga
     4681 attcgcgcat tatcgccatg cagggccggg ccctgctgga catcctgaag gacgccgtgg
     4741 tgctgctgaa tccggagggc ggcaagggcc tgctgttgaa cccggatcag atcaaggccg
     4801 tgctcggtca cgagccgatc cggccgctgc gcccggtttc cggccgcgtc gaactgcacg
     4861 acatcgagcc cgagaacgag ccggtcatgc tgaccctcgc catccagaaa tccttcaagg
     4921 gcgcgatggt caaggacgtc tggctggcgc gcgccctgtg gcccgacgtc ggtcagatcg
     4981 gctggttcct ggacatgcgt tcagacggtc ccgccgacga gatcaaggcc ctggtggtcc
     5041 gctcggtgcg tgggctgaag ttcggtacag agaccctgga cgtgtcggtc ggcaagccag
     5101 gcggcaagcc gggcgtgggc atgaagatca tctagaaccg caccacggtc cgcaccgcct
     5161 gcccgtcggc gagacggtcg aaggcggcgt tgatatccga cagggggacg acgccggtca
     5221 gcaggcggtc gaccggcagc cgaccttgcc ggaacagggc gacgtagcgc ggaatatcgc
     5281 ggcccggcac gcaggttcca atatagctgc ccttcagggt gcgctcctcg gcgaccagcg
     5341 cgacgacatt gacggccagc gccgcctcgg gcgggggcag gccggcggtg acggtggtcc
     5401 cgccgcggca ggtcatgcgc caggcggcgt cgagggcgcg gaccgagccg gccatctcga
     5461 atacgaaatc ggctccgccg ctggtcaggg cccggacctg ttcgacggcg tcggcgtcag
     5521 cggcgttgac cgtctggacg gcccccaggg tgcgggccag cgccagcttg tcctctgaca
     5581 gatcgaccgc gacgaccggc gaggcgccgg cggcgacggc gcccagcacc gacgacaggc
     5641 cgactccgcc cagaccgacc acgacgacgc tctggcccgc ccggacgccg gcggtgttca
     5701 cgaccgcgcc gacgcccgtc aggacggcgc agccgaacag ggcggcgtgt tcgaacggca
     5761 ggtccgcgtc gaccttcacc agcgaccggc gcgagacgac ggcatggctc gcgaacgccg
     5821 agcagccgag gtggtggttc aggtcgccag cgtcgtcatg gatgcgccgc ccgccggtca
     5881 gcagctcacc cttgccgttg gcggcggcgc cgggctcgca cagggcggga cggccctcgg
     5941 cgcacgggtt gcagtggccg caggagggca tgaagaccat gacgacatgg tcgccgacgg
     6001 ccagatcggt caccccgtcg cccagcgcct cgaccacccc ggcggcctcg tggcccaggg
     6061 ctatgggcag ggggcggggc cggtcgccgt tgatgacgct gaggtccgaa tggcacaggc
     6121 cggcggcctt gatcgccacc ataacctcgc cttggccggg cgcgtccagg gtcacggtct
     6181 cgatcgagag gggacgactg tccgcatagg gacgggcggc gcccatggtg cggagaacgg
     6241 cggcggtggt ctgcatgacg acacgctagg cgcttgacgc cgcgtccgcc aagcccaagg
     6301 ctgccctcaa gcgcgcacag acgcgccgga aacatcgcgc tcaagcagcg aggcgccgcg
     6361 cgccgagcga tagcgcacaa agggaagatt catgaccggt acgatcgagc ggacgaagcg
     6421 gggcgactat cgccggttcc aggccatctc cacacggtg
//
LOCUS       DASVVN010000413         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_251869,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000413 DASVVN010000000
VERSION     DASVVN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251869"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF686_06635"
     CDS             complement(<1..222)
                     /locus_tag="VF686_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX8661525.1"
                     /translation="MRSKLLLTGASIAVLLGGAACTTTDPYSSTPRRNNTATGALAGA
                     LGGAVLGYLTNTSNSEEGRKNALIGAGIGA"
     gene            439..1350
                     /locus_tag="VF686_06640"
     CDS             439..1350
                     /locus_tag="VF686_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX8661526.1"
                     /translation="MKLYDTDFQAFADKLGASFKRYGFAVIADHGLDEGVIEAAIADT
                     RTFFALPEETKRKYHQPGTGGARGLTPFGVEAAKGAATVDLKEFWHVGRELPEGHPYR
                     KYMRDNVWPTEVEGFRRHLYGMFAAMDELGAKILRAIARYLKLGDDFFADKVEMGNSI
                     LRMLHYPPVPAGASGVRAGAHEDINVITLLLGAEEAGLQLKDADGEWLDIAPPPGALV
                     VNIGDMLQRLTNHVLPSTSHRVINPAPERASFARYSTPFFLHFNPDFVIETLPSMISD
                     ENPDRYAGKAIMAEDFLTERLKEIRLI"
     gene            complement(1575..>3294)
                     /locus_tag="VF686_06645"
     CDS             complement(1575..>3294)
                     /locus_tag="VF686_06645"
                     /inference="COORDINATES: protein motif:HMM:NF027516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquitin-activating E1 FCCH domain-containing
                     protein"
                     /protein_id="HEX8661527.1"
                     /translation="LDAAALAAARSPYTANADLQRVGLASLRANLKAYPNITLREADT
                     SFVLNSDDVVIASSKVDVKTLVANIFLPPYGKFMDDYLPVGAHSEVDRSSKNIEVALV
                     LDITGSMAGQRIIDLKAAAKELVDLIVQPIQTPYYSKVALVPYSNSVNPGTHVTTARG
                     AIRGPVNVTGAVLNLIGTPMTITGATKGEPIVITSAAHGFSDGDVVWISGATGMTQLN
                     NKAYIIGGKTADTFKLYRLSGNRTNGENYGSYTGSAGRVQKCQNNDCSITVTAAAHGL
                     QNNDYVRIDEIVGMVNGNNDSMVNDRTFLAGNVTTNTFTIDPDASTMTPYISSGRAWR
                     AMGGGDTFFAFENMDGDLQTHRISGCVTERTGAQRYTEVAPTGASSRVAPQYPNLSGT
                     CIGDTIMPLSSNAATIKSRIDGFDVEGGTAGQIGIGWGWYMVSPDWNSLWPSGAAGAY
                     NTGETLKAVVIMTDGEFNTPYCSGVIARNAGSGSGGSASKIDCDADNGDPFTQSRALC
                     TAMKARGILVYTVGFQIAAGGDADNLLRACASTSSNYYNAGSGTALSQAFAAIGRDIT
                     QLRISR"
BASE COUNT          553 a         1012 c         1146 g          583 t
ORIGIN      
        1 cgcgccgatg ccggcgccga tcagggcgtt cttgcggcct tcctcagagt tggaggtgtt
       61 ggtcagatag cccagcaccg cgccgcccag ggcgccggcg agggcgccgg tggcggtgtt
      121 gttgcggcgc ggcgtggagc tgtaggggtc cgtggtcgtg caggcggcgc cgccgaggag
      181 gacggcgatg ctggcgccgg tgagaagaag cttgctgcgc atgtggaata tccctgaccg
      241 tgaattggct tgccgccgaa acgtgggctt acggtgaagg ttcccacctg tacggcttat
      301 gaacggcgcg ccgcggcttt gccaaggtct cgggttaggg cgtaaggaac cgggataccg
      361 aacctgcgag gccctgccgt gactgcgacc accaacagcg aaaccccgac cgcgaaacgc
      421 gccatcgagc cggtgtcgat gaagctgtac gacaccgatt tccaggcctt cgccgacaag
      481 ctgggagcct cgttcaaacg ctatggcttc gcggtcatcg ccgatcacgg tctggacgaa
      541 ggcgtgatcg aggcggccat cgccgacacc aggaccttct tcgccctgcc ggaggagacc
      601 aagcgcaaat accaccagcc gggcaccggc ggcgcgcgcg ggctgacccc gttcggcgtc
      661 gaggcggcga agggcgcggc cacggtcgat ctgaaggaat tctggcacgt cgggcgcgag
      721 ctgcccgagg gccatccgta ccgcaaatac atgcgcgaca acgtctggcc gacggaggtc
      781 gaaggtttcc ggcggcacct gtacggcatg ttcgccgcca tggacgaact gggcgcgaag
      841 atcctgcgcg ctatcgcccg ctatctgaag ctgggcgacg acttcttcgc ggacaaggtc
      901 gagatgggca acagcatcct gaggatgctg cactatccgc cggtgccggc gggcgcgtcg
      961 ggcgtgcggg ccggggcgca cgaggacatc aatgtcatca ccctgctgct gggcgccgag
     1021 gaggcgggcc tgcagctgaa ggacgccgac ggcgagtggc tggacatcgc gccgccgccg
     1081 ggcgcgctgg tggtcaatat cggcgacatg ctgcagcggc tgaccaacca cgtcctgccg
     1141 tcgaccagcc accgggtcat caatccggcg ccggagcggg ccagtttcgc ccgctactcc
     1201 acgcccttct tcctgcattt caacccggac ttcgtgatcg agaccctgcc cagcatgatc
     1261 tcggacgaga accccgaccg ctacgccggc aaggcgatca tggccgagga tttcctgacc
     1321 gaacggctga aggaaatccg gctgatctga gggcggcggg gcaaacgcaa aacacggtgc
     1381 acagggtgcg ctcggtgcac ccgccgacga gggcgccttt cgtcggcggg aaaaaacacc
     1441 gtccgaagcg tccgaagcgt ccgctcagtc cactgcgtcc gaacccgcgg acctttgcca
     1501 gcgcccaaaa accggaaccc gaaggttcgg gcagtgaagc atgcgcctgc gtcaaatttg
     1561 gtcgtgcggg cgttctatcg ggatatccgc agctgggtga tgtcgcgacc gatggcggcg
     1621 aaggcctggg acagagccgt gccgctgccg gcgttgtaat agttgctgga cgtcgaggcg
     1681 caggcccgga ggagattgtc agcgtcgccg cccgcagcga tctggaagcc taccgtgtag
     1741 acgagaatgc ccctcgcctt catggcggtg catagagcgc ggctctgggt gaagggatcg
     1801 ccgttgtccg cgtcgcagtc gatcttcgag gcgctgccgc cggacccgga acccgcattg
     1861 cgggcgatga cgcccgagca gtagggggtg ttgaactcgc catcggtcat gatgaccacg
     1921 gctttcaggg tctcgccggt attgtaggcg cctgccgcgc cgctgggcca caggctgttc
     1981 cagtcggggg agaccatgta ccagccccag ccgatgccga tctggcccgc ggtcccgcct
     2041 tcgacgtcaa agccgtcgat gcgtgacttg atcgtggccg cattgctgga caggggcatg
     2101 atcgtatccc cgatgcatgt gccgctgagg ttcggatatt gcggcgccac ccgggatgac
     2161 gcgcctgtcg gcgcaacctc ggtatagcgt tgcgcgcccg tccgctcggt gacgcaaccg
     2221 ctgatgcggt gggtctgcag atccccgtcc atgttctcga aggcgaagaa ggtgtcgccg
     2281 ccgcccatgg cgcgccaggc ccggccgctg gagatgtagg gcgtcatcgt cgacgcgtcg
     2341 ggatcgatgg tgaaggtgtt ggtcgtgacg ttgcccgcca ggaaggtcct gtcgttgacc
     2401 atgctgtcgt tgtttccatt gaccatcccg acaatctcgt cgatccggac atagtcattg
     2461 ttctgcaggc cgtgcgccgc ggcggtgacg gtgatggaac agtcgttgtt ctggcatttc
     2521 tgaacccggc cggcgctgcc cgtataggag ccgtagttct cgccgttggt cctgttgccg
     2581 ctgaggcggt agagcttgaa ggtgtcggcc gtcttgccgc cgatgatata ggccttgttg
     2641 ttcagctggg tcatgccggt cgccccgctg atccagacca cgtctccgtc cgaaaagccg
     2701 tgcgcggcgg aggtgatgac gatcggctcg cccttggtgg cgccggtaat cgtcatcgga
     2761 gtgccgatca ggttcagcac agcgccggtg acgttcacag ggccgcgaat cgcgccgcgc
     2821 gccgtcgtga catgggtccc cggattgacc gagttcgaat agggcaccag ggcgaccttg
     2881 gaatagtagg gggtctggat cggctggacg atcaggtcga ccagttcctt ggcggccgcc
     2941 ttcaggtcga tgatccgctg tccggccatc gaaccggtga tgtcgagcac gagggcgacc
     3001 tcgatattct tggacgaccg gtcgacctcg gagtgggcgc ccacgggcag gtagtcgtcc
     3061 atgaacttgc cgtacggcgg caggaagatg ttggcgacca gggtcttcac atcgaccttt
     3121 gaactggcga tcacgacgtc gtcgctgttc aggacgaagc tggtgtcggc ctcacgcagg
     3181 gtgatgttgg gataggcctt caggttggcc ctcagcgaag caagcccgac ccgctgaagg
     3241 tcggcgttgg ccgtgtaggg cgaacgggcg gcggcgaggg cggcggcgtc gagc
//
LOCUS       DASVVN010000414         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_174578,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000414 DASVVN010000000
VERSION     DASVVN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174578"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF686_06650"
     CDS             <1..337
                     /locus_tag="VF686_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HEX8661528.1"
                     /translation="CDAVNAAKAARGGDQAWDPAPYRGEQGDGIWVCPAALETPFSLA
                     PGDTAGKAGGLVFLIDPYVVGPYAEGVYTAVVPAAAFSALLWPEWADQFGGAPSPEYL
                     DRLRGEQAP"
     gene            361..1230
                     /locus_tag="VF686_06655"
     CDS             361..1230
                     /locus_tag="VF686_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEX8661529.1"
                     /translation="MTSIARPPLRLLLLTAALAAALTGCNRDREPAAPAPAAPGTAAV
                     VTPADAAAPMAFESKTPWADVRLTLPVGIKPQTDLHARLYAEEVRRLRQFTEGAQGEL
                     TEAGAETDRPKYEKTITITVAAETAKLFSLKRVDFDYSGGAHPNTLTDGLLWDKALKR
                     RVGVAELFGKGADLTILDQALCSAINAAKRARVPDSAGVTLDGGKTFSCPRAADTPFI
                     LTAGDTPGKAAGLTFLIGPYQVGPYVEGGYEIAIPATAFRSLLAVAYSSEFGGRLTKA
                     GDVTPVVAAVATT"
     gene            1317..1832
                     /locus_tag="VF686_06660"
     CDS             1317..1832
                     /locus_tag="VF686_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661530.1"
                     /translation="MRTFTMIAVCAASLIAMPALAQQGAGVNVWHEGNLNDGSGVTAM
                     GPNSGGLLATTPSRISSSRREMNRGRESNWARRPTPAQLRRSTELAIERGGLHCTIAE
                     MDMVGQLTDGTPLVEVACQENGGVVIANSTPVQATDCFDLVGSTGVLAPCRIPANIAL
                     VEAALQSASKN"
     gene            complement(1811..>2282)
                     /locus_tag="VF686_06665"
     CDS             complement(1811..>2282)
                     /locus_tag="VF686_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661531.1"
                     /translation="DSLPFFSALFGPTATVESARPFAQQAAAGILAADAASYRAGQEQ
                     AAVGYSRTPATRAAMVDWSMTSDRRAMASAISEVMTTDLRPGLAAMTTPVWAVYASDA
                     DGGAPAAMADALWAREYAGLPGVRLIRIDGSRHFIMADQPERFAGLVDQFLLAD"
BASE COUNT          364 a          882 c          725 g          311 t
ORIGIN      
        1 ctgcgacgcc gtcaacgccg ccaaggcagc ccggggcggc gaccaggcct gggatcccgc
       61 gccatatcgc ggcgaacagg gcgacggcat ctgggtctgc cccgcagcgc tcgaaactcc
      121 gttctccctc gcccccggcg acacggcggg caaggccggg ggtctggtct tcctgatcga
      181 cccctatgtc gtcggcccct atgccgaggg cgtctacacc gccgtcgtgc cggcggccgc
      241 cttctccgcc ctgctgtggc ccgaatgggc cgatcagttc gggggcgcgc cctcgccgga
      301 ataccttgac cggcttcgcg gtgagcaagc accatgacgg cgacgcatca ggagacaccc
      361 atgacctcga tcgcccggcc gcccctccgg ctgttgctcc tgaccgccgc tctcgccgcg
      421 gctctgaccg gctgcaaccg ggaccgcgaa cccgccgccc ctgcgcccgc cgcgccggga
      481 accgccgccg tcgtcacccc cgccgacgcc gccgccccga tggccttcga gagcaagacc
      541 ccctgggccg acgtccgcct gaccctgccc gtcggcatca agccgcagac cgacctgcac
      601 gcccgcctct acgccgagga ggtgcgccgc ctgcgccagt tcaccgaggg cgcccaaggc
      661 gagctgaccg aggccggggc cgagaccgac cggcccaaat acgaaaagac catcaccatc
      721 acggtcgccg ccgagaccgc caaactgttc agcctcaagc gcgtcgactt cgactattcg
      781 ggcggcgccc atcccaacac cctcaccgac ggcctgctgt gggacaaggc gctgaagcgc
      841 cgggtcggcg tcgccgagtt gttcggcaag ggcgcggacc tcaccatcct ggatcaggca
      901 ctgtgctcgg cgatcaacgc cgccaaacgc gcccgcgtgc ccgacagcgc cggcgtgacc
      961 ctggacggcg gcaagacctt ctcctgcccc cgcgccgccg acacgccgtt catcctgacg
     1021 gcgggcgata cgccgggcaa ggcggctggc ctgacctttc tgatcggtcc gtatcaggtc
     1081 ggtccctatg tcgagggcgg ctatgagatc gccattcccg ccacggcctt ccgctccctg
     1141 ctggccgtcg cctacagcag cgagttcgga ggccgtctga ccaaggccgg agacgtcacc
     1201 ccggtggtcg ccgccgtcgc cacaacctga cgggcggccg gcaagacagt acaagcccga
     1261 tttgcataac cgcgattttc gcgagaaggt gcgcgaacaa ctcagggaga ccctcgatgc
     1321 gcaccttcac catgatcgcc gtctgcgccg cttcactgat cgccatgccg gctctcgccc
     1381 agcagggcgc cggagtgaac gtctggcacg agggcaatct caacgacggc agcggcgtca
     1441 ccgccatggg tccgaacagc ggcggactgc tcgccaccac gcctagccgg atctccagct
     1501 cgcgccgcga gatgaaccgg ggccgcgaga gcaactgggc ccgccgcccg acgccggccc
     1561 aactgcgacg gtccactgaa ctggccatcg agcgcggcgg acttcactgc acgatcgccg
     1621 aaatggacat ggtcggccag ctcacggacg gcaccccgct ggtggaggtc gcctgccagg
     1681 agaacggcgg cgtcgtcatc gccaactcga cgcccgtcca ggcgaccgac tgcttcgatc
     1741 tggtcggatc caccggcgtc ctcgcgccgt gccggattcc cgccaacatc gccctggtcg
     1801 aggctgcgct tcagtccgcc agcaagaact gatcgaccag gccggcgaac cgctccggct
     1861 gatcagccat gatgaaatga cggctgccgt cgatgcggat caagcgcacg ccgggcagac
     1921 ctgcatactc tctcgcccac agtgcgtcgg ccattgcggc cggcgcgccg ccatccgcgt
     1981 ctgaagcgta gacggcccag accggggtcg tcatcgccgc cagcccggga cgcaggtcgg
     2041 tcgtcatcac ctcgctgatc gccgaggcca tggcgcgccg gtccgaggtc atcgaccagt
     2101 cgaccatggc ggcgcgggtc gccggcgtcc gcgaatagcc gaccgcggcc tgctcctgac
     2161 cggcgcggta gctcgccgca tccgccgcca ggatccccgc cgccgcctgc tgcgcgaacg
     2221 gccgggcgct ctcgaccgtc gccgtgggtc cgaacagggc cgaaaagaac ggcaggctgt
     2281 cg
//
LOCUS       DASVVN010000415         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_174650,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000415 DASVVN010000000
VERSION     DASVVN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174650"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="rlmN"
                     /locus_tag="VF686_06670"
     CDS             <1..670
                     /gene="rlmN"
                     /locus_tag="VF686_06670"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX8661532.1"
                     /translation="LEEWPSPKEDRRLSNIVFMGMGEPLYNLDHVADAIDIISDNEGI
                     ALSRRRITVSTSGVVPQLDALGTRTQAMLAISLHATNDALRDVLVPLNKKYDLAQLMA
                     GIRAYPGISNARRVTFEYVMLKGVNDSPEEARALLKLIEGIPAKVNLIPFNPWPGTDY
                     ECSDWKTIERFAAILNKAGYASPIRTPRGRDILAACGQLKSESEKVRASTLRKAEQAS
                     VEVA"
     gene            779..1222
                     /locus_tag="VF686_06675"
     CDS             779..1222
                     /locus_tag="VF686_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX8661533.1"
                     /translation="MDSPSLSTLLDSAEVQAFATGFPTTMAHLGVTLALMLGGAVIYA
                     LFTPWKEITLIRQGNAAAAVAFSGVLIGLAIPLAVSLSVSTSIKDIALWGVATVVIQL
                     LAFRLVDMILTGLPQRIREGEVSAAVLLVGAKISTALILAAALTG"
     gene            1227..>1818
                     /locus_tag="VF686_06680"
     CDS             1227..>1818
                     /locus_tag="VF686_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8661534.1"
                     /translation="MGFPRPPDWLIYTAVLGGLLMLSLGRRENADAPPAPPPPDEAEG
                     ALLGPITPFDPAVTVNAPDIPFQPASGTAFSISREGRWVTARHVVEGCRRPAILVGGG
                     RAVAADVRLAPRADIAVLLTQGGADAVPVSALQSLRTGQRAFHPGYPQARAGEVTSRL
                     LGRETLRVRGRGQRDEPVLAWAEVGRTDGLGGTLAGL"
BASE COUNT          260 a          699 c          583 g          276 t
ORIGIN      
        1 cctcgaggaa tggccgtcgc ccaaggaaga ccgccgcctg tccaacatcg tgttcatggg
       61 catgggcgag ccgctctaca atctggacca cgtcgccgac gccatcgaca tcatctcgga
      121 caacgagggc atcgccctgt cgcggcgccg gatcacggtc tcgacctcgg gcgtggtgcc
      181 gcagctcgac gcgctcggaa cccgcaccca ggccatgctg gcgatcagcc tgcacgccac
      241 caacgacgcc ctgcgcgacg tgctggtgcc gctgaacaag aagtacgatc tggcccagct
      301 gatggccggc atccgcgcct atccgggcat ttccaacgca cgtcgggtga ccttcgaata
      361 cgtgatgctc aagggcgtca acgacagccc ggaagaagcc agggcgttgc tgaaactgat
      421 cgagggcatt ccggccaagg tgaacctgat cccgttcaat ccctggcccg ggaccgacta
      481 cgaatgctcg gactggaaga cgatcgagcg gttcgcggcc atcctgaaca aggccggcta
      541 cgcctcgccc atccgcaccc cccgcggccg cgacatcctc gccgcctgcg gccagctgaa
      601 gtccgagagc gagaaggtgc gggcgtccac cctgcgcaag gccgaacagg cttccgtcga
      661 ggtcgcctaa cctccggtta caaggcgaca agattcgtga ccgggtgtgg cgccgtcgcg
      721 cttgcggatt ggggcgaaaa ccgggcaatc gtcggggacc ttctgacccc ggatcgccat
      781 ggactccccg tccctctcca ccctgctcga cagcgccgag gtccaggcct ttgcgaccgg
      841 ctttcccacc accatggcgc acctcggggt gaccctggcg ctgatgctgg gcggagccgt
      901 gatctacgcc ctgttcacgc cgtggaagga gatcaccctg atccgtcagg gcaacgccgc
      961 cgcggccgtg gccttctcgg gcgtgctgat cggtctcgcc atcccgctgg cggtcagcct
     1021 cagcgtctcg acctcgatca aggacatcgc cctgtggggc gtggccaccg tggtgatcca
     1081 gctgctggcc ttccgcctcg tcgacatgat cctgaccggc ctgccccagc gcatccgcga
     1141 gggcgaggtc tcggccgccg tcctgctggt cggggccaag atctcgaccg ccctgatcct
     1201 cgccgccgcc ctgacgggct gacgccatgg gttttccccg cccgcccgac tggctgatct
     1261 atacggcggt cctcggcggc ctgctcatgc tgtcgctggg ccgccgcgag aacgccgacg
     1321 ccccgcccgc cccgccgccg cccgacgagg ccgaaggagc cctgctgggc ccgatcacgc
     1381 ccttcgatcc ggccgtcacc gtcaacgccc ccgacatccc cttccagccc gcctcgggca
     1441 ccgccttctc gatctcgcgc gagggccgct gggtcaccgc ccgtcacgtg gtcgagggct
     1501 gccgccggcc cgccatcctc gtcggcggcg gccgcgccgt ggccgccgac gtgcgtctgg
     1561 ccccccgcgc cgacatcgcc gtgctgctga cccagggcgg ggccgacgcc gtgccggtct
     1621 ccgccctcca gagcctgcgc accggccagc gcgccttcca ccccggctat ccgcaggccc
     1681 gcgccggcga ggtcacctcc cgccttctcg gccgcgaaac gctgagggtg cgcgggcgcg
     1741 gccagcggga cgagccggtc ctggcctggg ccgaggtcgg ccgcaccgac ggtctgggcg
     1801 gcacgctggc gggtctgt
//
LOCUS       DASVVN010000416         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_174655,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000416 DASVVN010000000
VERSION     DASVVN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174655"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VF686_06685"
     CDS             complement(<1..1560)
                     /locus_tag="VF686_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HEX8661535.1"
                     /translation="MIRTFGLVSALALATALGGCASMPGFGPSSGSDMPPLPASAAAP
                     AEYARDTHSYARPEIAHVRHVALDLTADFEAHTLSGTAGLDIQGVQGADEIVLDVKNL
                     DIRNVTDAAGAPLQYETGPNDAIKGQPLTVRFPALTAGETRRIVVAYSTRPDAAALQW
                     LTPSQTAGGQKPYLFSQGQAILTRTWVPTQDSPGIRQTYDARIVAREDLRVVMSAQQL
                     TPEGEAAGPGMKAWRFRMTNPVPPYLIAIGVGDLAFAAIDERTGVFTEPSRLEAARAE
                     LAPTADMVDAAERLYGPYRWGRYDLLVLPPSFPFGGMENPRLTFATPTIIAGDQSLVS
                     LVAHELAHSWSGNLVTNATWNDMWLNEGFTVYFENRIMEALYGEDRALMLRALGWGDL
                     QTEMAGLPPADTRLHLDLEGRDPDEGLTDVAYEKGAAFLRTIERIVGRERFDAWLNGY
                     FERNAFRPMTTDLFLQDIRTHLVAGDAGLERQLMMDAWIYQPGMPSNWQPPVSGAFGP
                     VDAAARAFFADK"
BASE COUNT          242 a          518 c          618 g          254 t
ORIGIN      
        1 cttgtccgcg aagaaggcgc gggcggccgc atcgactgga ccgaaggcgc ccgacaccgg
       61 cggctgccag ttcgacggca tgccgggctg atagatccag gcgtccatca tcagctggcg
      121 ttccagaccg gcgtcgccgg cgaccagatg cgtgcggatg tcctgcagga acaggtcggt
      181 ggtcatgggg cggaaggcgt tgcgctcgaa atagccgttc agccaggcgt cgaaccgctc
      241 gcgcccgacg atccgctcga tggtgcgcag gaacgcggcg cccttctcat aggcgacgtc
      301 ggtcaggccc tcgtcgggat cgcggccctc gaggtccagg tgcaggcggg tgtcggcggg
      361 cggcaggccg gccatctcgg tctgcagatc gccccacccc aaggcgcgca gcatcagggc
      421 gcggtcctcg ccgtagaggg cctccatgat gcggttctcg aaatagacgg tgaacccctc
      481 gttcagccac atgtcgttcc aggtggcgtt ggtgacgagg ttgcccgacc agctgtgcgc
      541 cagctcatgc gccaccagcg agaccagcga ctggtcgccg gcgatgatgg tcggggtggc
      601 gaaggtcagg cgcgggttct ccattccccc gaacgggaag gagggtggca ggaccagcag
      661 gtcgtaccgg ccccagcgat aggggccgta caggcgctcg gcggcgtcga ccatgtcggc
      721 ggtcggggcc agttcggcgc gggccgcctc cagacgtgat ggctcggtaa agacgcctgt
      781 gcgctcgtcg atggcggcga aggccaggtc gcccacgccg atggcgatca gatagggcgg
      841 gaccggattg gtcatgcgga accgccaggc cttcatgccc ggaccggcgg cctcgccctc
      901 gggcgtcagc tgctgggcgc tcatgacgac gcgcaagtcc tcgcgggcga cgatgcgggc
      961 gtcataggtc tgccggatgc cggggctgtc ctgcgtcggc acccatgtgc gggtcaggat
     1021 cgcctgaccc tgactgaaca gatagggctt ctggccgccc gcggtctggg agggcgtcag
     1081 ccattgcaag gcggcggcgt ccgggcgggt cgaataggcg accacgatgc ggcgcgtctc
     1141 gcccgctgtc agggccggga agcgcacggt caggggctgg cccttgatgg cgtcgttcgg
     1201 gccggtctcg tactgcagcg gcgccccggc ggcgtcggtg acgtttcgga tgtcgagatt
     1261 ttttacatcc agcacgatct cgtcggcgcc ctgaacgccc tggatgtcga gcccggcggt
     1321 tcccgacagg gtgtgggcct cgaagtcggc ggtcaggtcc agggcgacgt ggcgcacatg
     1381 ggcgatctcg ggccgggcgt agctgtgggt gtcgcgcgcg tattcggcgg gcgcggcggc
     1441 cgaggccggc aggggcggca tgtccgagcc ggacgagggg ccaaagccgg gcatcgaggc
     1501 gcatcctccc agagccgtcg ccagagcgag ggcggagacc agtccaaacg tgcggatcat
     1561 cgagagtcct tgaccagaag gcccgccatc cttgttcggg cggtcgggcc gggttcgttt
     1621 cctgccggga ca
//
LOCUS       DASVVN010000417         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_226287,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000417 DASVVN010000000
VERSION     DASVVN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226287"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF686_06690"
     CDS             complement(<1..142)
                     /locus_tag="VF686_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX8661536.1"
                     /translation="MATVTVEINGRPYAVGCADGQEERVRTLASQFDGHVRQVAGEVG
                     HVG"
     gene            complement(142..399)
                     /locus_tag="VF686_06695"
     CDS             complement(142..399)
                     /locus_tag="VF686_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661537.1"
                     /translation="MDAASAAKDRVDRALALLERRLLDLKGRAAGSSRVPDDDLFAPE
                     PSTETDRLRIHELEAAGRDAAEVLSRAADAIRESLAEQEAR"
     gene            543..>2025
                     /locus_tag="VF686_06700"
     CDS             543..>2025
                     /locus_tag="VF686_06700"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8661538.1"
                     /translation="MADAIRVLSMDGVEKAKSGHPGMPMGMADVATVLFSKFLKFDAS
                     RPDWADRDRFILSAGHGSMLIYSLLHLTGYKAATKEELSNFRQWGSKTAGHPEFGHMP
                     GVETTTGPLGQGLANSIGFAMAERHLAARFGDDLVDHRTWVIAGDGCLMEGVSQEAIT
                     LAGRYRLNKLTVLWDDNAITIDGAVSLSDATDQKARFKAAGWAVKAIDGHDMKAIKSA
                     LQWATRQDKPTLIACKTKIGRGAATMEGSHKTHGAALGAAEIAATRLGLSWTHEPFEL
                     PEAVDKAWKKVGRRGAKERKAWETRLNASSQAADFTRAMKGDLPAGAFDKLQAKIAEL
                     VETKPAQATRQSSGAALDELFAAIPELIGGSADLTGSNNTFVKGTPIFDAPTYEGRYV
                     NWGIREFGMAAAMNGMALHGGVIPYGGTFMVFSDYSRPAIRLGALMGVRAIHVLTHDS
                     IGLGEDGPTHQPVEQLVSLRCIPNLVVLRPADATETAEAWRTAL"
BASE COUNT          348 a          682 c          702 g          293 t
ORIGIN      
        1 cgccgacatg gccgacctcg cccgccactt gacgcacatg gccgtcgaac tggctggcga
       61 gcgtgcggac gcgctcttcc tggccgtcgg cgcaaccgac ggcgtagggg cggccgttga
      121 tctcgacggt gaccgtcgcc atcagcgggc ctcctgttcg gccaagctct cgcggatggc
      181 gtcggcggcg cgggacagga cctcggcggc gtcgcggccg gcggcctcaa gctcgtggat
      241 gcgcaggcgg tcggtttcgg tcgagggctc aggcgcgaac agatcgtcgt ccggcacccg
      301 gctggatccg gccgcgcgtc ccttgaggtc cagcaggcgg cgctcgagca gggcgagcgc
      361 gcggtcgaca cggtccttgg cggccgaagc agcatccatt cgggtcacat cctccagaat
      421 ccgtatagaa cccgcgcgca cgccggggta aagcggcgcg ctttcttttt tccgcccccc
      481 gacgaaaggt cctccccgtg accgacgcca aaaccgcatc cgaaaaggcc acccccagac
      541 agatggccga cgccatccgg gtgctgtcaa tggacggggt cgagaaggcg aagagcggcc
      601 accccggcat gccgatgggc atggccgacg tggcgacggt actgttttcg aaatttctga
      661 agttcgacgc cagccgcccc gactgggccg accgcgaccg cttcattctg tcagcgggcc
      721 acgggtcgat gctgatctac agcctgttgc acctgaccgg ttacaaggcg gcgacgaagg
      781 aagagctgtc gaatttccgc cagtggggat cgaagacggc cggccacccc gagttcggcc
      841 atatgccggg cgtggaaacc accacgggac cgctgggcca gggcctggcc aacagcatcg
      901 gcttcgccat ggccgagcgc catctggcgg cgcggtttgg tgatgacctt gtcgaccacc
      961 ggacctgggt gatcgcgggc gacggctgtc tgatggaagg cgtgtcgcag gaggcgatca
     1021 cgctggccgg ccgctatcgg ctgaacaagc tgacggtgct gtgggacgat aacgccatca
     1081 ccatcgacgg cgcggtgtcg ctgtcggacg ccacggacca gaaggcgcgc ttcaaggccg
     1141 cgggctgggc cgtgaaggcg atcgacggcc atgacatgaa ggcgatcaag tccgcgctgc
     1201 agtgggcgac ccgtcaggac aagccgacgc tgatcgcctg caagaccaag atcggccgcg
     1261 gcgccgccac catggaaggc agccacaaga cgcacggcgc ggcccttggc gctgccgaga
     1321 tcgccgccac ccgtctgggc ctgtcctgga cgcatgaacc gttcgaactg cccgaagccg
     1381 tcgacaaggc gtggaagaag gtcggacgcc ggggcgccaa ggagcgcaag gcatgggaaa
     1441 cccgcctgaa cgcctcgtcc caggccgccg acttcacccg cgcgatgaag ggcgacctgc
     1501 ctgccggcgc cttcgacaag ctgcaggcga agatcgccga gctggtcgag acgaagccgg
     1561 cacaagccac gcgccagtcg tcgggcgcgg cgctggacga gctgttcgcc gccattcccg
     1621 aactgatcgg agggtcggcc gatctgaccg gttcgaacaa caccttcgtc aagggcacgc
     1681 cgatcttcga cgcgccgacc tatgagggcc gctacgtcaa ctggggcatc cgcgagttcg
     1741 gcatggccgc ggcgatgaac ggcatggcgc tgcacggcgg ggtcatcccc tacggcggca
     1801 ccttcatggt gttttcggac tacagccgtc cggcgatccg gctgggcgcc ctgatggggg
     1861 tgcgggcgat ccacgtgctg acccacgact cgatcggtct gggcgaagac ggcccgacgc
     1921 accagccggt cgagcagctc gtgtcgttgc gctgcatccc gaacctcgtg gtgctgcgcc
     1981 ccgccgacgc gacggagacg gccgaagcgt ggcgcaccgc gctca
//
LOCUS       DASVVN010000418         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_361475,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000418 DASVVN010000000
VERSION     DASVVN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361475"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1777)
                     /locus_tag="VF686_06705"
     CDS             complement(32..1777)
                     /locus_tag="VF686_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661539.1"
                     /translation="MTDANTPAKPVRGLGGLAVYGERRVFVMLLLGFSAGLPNLLIFD
                     TLSTWLRDAGLTLEVIGFFALATLTYALKFVWAPLIDRTSVPWLTKLLGHRRSWMLVT
                     QAVIILGLWLIAGTDPTKALGAMAAFAVMVGFFGASQDIVIDAWRIESADDSRQGAMA
                     AAYQWGYRIALIIAGAVPLILADLYNWNFSYAVMAALMGLGVLGVLLAPREQAHTPRA
                     IPVADIPSRPLIEKIEWVLRLAVLLIAAIVIGTGLTDKPDALLWAFSPVLDESATGMI
                     TAGLKDNELSGVLLQFAYVIGGFAMLVAACWPLPRYRTRPGAYLAGSFGQPLKDFFQR
                     FGHLAMPILALICCYRLADFVLNIMPPFYLDLGFTLTEIAEVRKVFGVIMTSLGVFVG
                     GWSVARLGLIRTMVIGAFASPASNLIFAWLATQGHDLSALFVAIGVDNLATGYAGTAL
                     IAYMSSLTSLGFTATQYALFSSLYALPGKIVASQSGKIVEASARAADAGGPLAVLKPL
                     FSGMTPESLIGGAAKSGVTPTGLGAGYVTFFLYSTVIGLFAILLAFYVAKRQPAVMAA
                     QKEREAEASTEAQPS"
BASE COUNT          370 a          602 c          589 g          269 t
ORIGIN      
        1 tgatctatcg ggtctttgac attgtgaacg gtcaggacgg ctgggcctcg gtggacgcct
       61 cggcctcacg ctccttttgc gccgccatca ccgccggctg gcgtttggcg acatagaagg
      121 ccagcaggat ggcgaacaag ccgatgacgg tcgagtagag gaagaaggtc acatagcccg
      181 cccccagccc ggtcggcgtg acgccgctct tggccgcgcc gccgatcagc gactcaggcg
      241 tcatacctga gaacagcggc ttcagcaccg ccaagggtcc gcccgcgtcg gccgcccttg
      301 cggacgcctc gacgatcttg cccgactggc tcgccacgat ttttccgggc agggcgtaca
      361 gcgagctgaa cagggcgtac tgcgtcgccg tgaatcccaa cgaggtcagg ctggacatat
      421 aggcgatcag agctgtgccg gcgtagccgg tggcgagatt gtcgacgccg atcgccacga
      481 acagggcgct caggtcgtgc ccctgtgtcg ccagccaggc gaagatcaga ttcgacgccg
      541 ggctggcgaa tgcgccgatg accatcgtcc ggatcagccc cagccgcgcc accgaccaac
      601 ccccgacgaa gacgcccagc gaggtcatga tcaccccgaa caccttccgc acctcggcga
      661 tctcggtgag ggtgaagccg aggtcgagat agaagggcgg catgatattc agcacgaagt
      721 ccgccaaccg gtagcagcag atcagcgcca gaatcggcat ggccagatgg ccgaaccgct
      781 ggaagaaatc tttcagcggc tgaccgaacg accccgccag ataggccccc ggccgggtgc
      841 gatatcgcgg cagcggccag caggcggcga ccaacatggc gaaaccgcca atcacatagg
      901 cgaattgcag cagaacgccg gacagctcgt tgtccttcag cccggctgtg atcatgcccg
      961 tggcgctctc gtccaataca gggctgaacg cccacaggag cgcgtccggc ttgtcggtca
     1021 gtccggttcc gataacgatg gcggcgatca gaagcaccgc cagccgcaag acccattcga
     1081 tcttttcgat gaggggccgt gacggaatgt cggcgaccgg gatggccctc ggggtatggg
     1141 cctgctcccg cggagccagc aggacgccca gaacgcccag acccataagg gcggccatga
     1201 cggcgtacga gaaattccag ttgtacagat ctgcgaggat cagcggcacg gcgccggcga
     1261 tgatcagggc gatgcgatat ccccactgat aggcggcggc cattgcgccc tgccggctgt
     1321 catcggcgga ctcgatccgc caggcgtcga tgacgatatc ctgactggcc ccgaagaagc
     1381 ccaccatgac ggcgaaagcc gccatggctc ccagcgcctt cgtcgggtcg gtgccggcga
     1441 tcagccacag accgagaatg atcacggcct gggtcacaag catccatgag cggcgatgcc
     1501 ccagcagctt tgtcagccac ggcacgctgg tgcggtcgat caacggcgcc cagacgaact
     1561 tcagggcata ggtcagggtg gcgagggcga agaagccgat aacctcgagc gtcagacccg
     1621 cgtcacgcaa ccacgtcgac agggtgtcga agatgagcag attcggtaac cccgccgaga
     1681 agccgagcag cagcatgacg aacacgcgcc gctcgccata gaccgccaga cccccgaggc
     1741 cgcgcacggg tttggcgggc gtattggcgt cggtcatcgg tgcgtccctc gaagcgacgc
     1801 ccccgcgtcc cctatgcgac ggcgaccttg
//
LOCUS       DASVVN010000419         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_361487,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000419 DASVVN010000000
VERSION     DASVVN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361487"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF686_06710"
     CDS             complement(<1..299)
                     /locus_tag="VF686_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX8661540.1"
                     /translation="MAVAPTPVQITAARDALIARDPALARAHAQTPVFDWRWRTGGFE
                     GLFHMIVDQQVSVASAAAIWARVEAGLGGDVTPEKVLATDIETLRSFGLSIQKAT"
     gene            387..1235
                     /gene="gluQRS"
                     /locus_tag="VF686_06715"
     CDS             387..1235
                     /gene="gluQRS"
                     /locus_tag="VF686_06715"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX8661541.1"
                     /translation="MFTTRFAPSPTGRLHKGHAFSALMAWTAAREAGGRFRLRIEDID
                     PVRCRPEFEAAILDDLAWLGLDWEQPVRRQSDHLTDYASVVDVLDRRGLLYRCFRTRK
                     DILDAIADAPHGPVEAARPGPHPAEEEAAFLAEGRPFAWRLSLDRARDLLGQRAWEAL
                     AFIEEGAGPDGERGLIRARPETAGDVVLARKDAGTAYHVAVTHDDALQGVTHVIRGHD
                     LFEATHIQVLIQTLMGWPTPVYRHHALLVGEDGRRYAKRDRSVTLDELRAAGLSPETL
                     RAELGF"
     gene            complement(1236..1985)
                     /gene="ppk2"
                     /locus_tag="VF686_06720"
     CDS             complement(1236..1985)
                     /gene="ppk2"
                     /locus_tag="VF686_06720"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX8661542.1"
                     /translation="MGKNTDDYQTELERLQLKLVEAQAWAIEKGQKTVMVFEGRDAAG
                     KDGSITRLTEYMSPRQTRVVALPKPTERETGQWYFQRYVPHLPTEGETSIFNRSWYNR
                     AGVEPVMGFCTPEQHEQFLKDAPRFERLLTDSGILLIKFWFDISKEEQARRLDDRAAE
                     PLKRFKRSPLDAEAQKRWDAYSAARDRMLAETHSDHAPWTVVATDHKKTARLNVIRHV
                     LNRLDAPGCAEEKPDKDVIFPASKADGRLTP"
BASE COUNT          324 a          760 c          750 g          375 t
ORIGIN      
        1 taggtcgcct tctggatcga cagtccgaaa ctgcgcaggg tttcgatatc ggtggccaac
       61 accttctccg gcgtcacgtc gccgccgagg cccgcctcga cgcgggccca gatcgcagcg
      121 gccgaggcga ccgaaacctg ctggtccacg atcatgtgga acagaccctc gaatccgccg
      181 gtgcgccagc gccagtcgaa gaccggagtc tgggcgtggg cgcgcgcgag ggccggatcc
      241 cgtgcgatga gggcgtcgcg cgcggcggtg atctgaacgg gggtgggagc aacagccatg
      301 gccgaccgtg acgatccctg aggctggcga caagccgtgc ggttcaaccc ctgacgatca
      361 ggtccgcttc ccgctatcat cggtccatgt tcacgacgcg cttcgcccct tcgccgaccg
      421 gtcgccttca caagggccac gccttctcgg ccctgatggc ctggacggcg gcgcgagagg
      481 cgggcggacg attccggctg cggatcgagg acatcgatcc cgtgcgatgc cgacccgagt
      541 tcgaggccgc gattctggac gatctggcct ggctgggtct ggactgggaa cagcccgtgc
      601 ggcggcagtc ggatcatctg accgactacg cttccgtcgt cgacgtgctg gaccggcgcg
      661 gtctgctcta ccgctgcttc cgcacccgca aggatatcct ggacgccatc gccgatgcgc
      721 ctcacgggcc cgtcgaggcg gcgcggcccg gtccgcatcc ggcggaggag gaggccgcct
      781 tcctcgccga gggacgcccc ttcgcctggc ggctatcgct ggaccgggct cgcgacctgt
      841 tgggccaacg cgcatgggag gcgctggcct tcatcgagga gggcgcgggt cccgacggcg
      901 aacgcggcct gatccgggcg cggccggaga cggccggcga cgtggtcctg gcccgcaagg
      961 acgccgggac cgcctaccat gtcgcggtca cccacgacga cgccctgcaa ggcgtgaccc
     1021 atgtcatccg cggtcacgac ctgttcgagg cgacccatat ccaggtgctg atccagacgc
     1081 tgatgggctg gcccacgccg gtctaccggc accacgccct gctggtcggc gaagacgggc
     1141 ggcgctacgc caagcgcgac cggtccgtca cgctcgacga gctgcgcgcc gcgggcctga
     1201 gcccggaaac cctgcgggcc gaactgggct tctgatcagg gggtcagccg accatcggcc
     1261 ttgcttgccg ggaagatcac atccttgtct ggcttctcct cggcgcagcc cggcgcatcc
     1321 agccggttca ggacatgccg gatgacgttc aggcgagcgg tcttcttgtg gtcggtggcg
     1381 acgaccgtcc acggcgcatg gtcactgtgg gtctcggcca gcatccggtc gcgcgccgcc
     1441 gaataggcgt cccatcgctt ctgcgcctcg gcgtccagcg gcgaccgctt gaaccgcttc
     1501 agcggctccg ccgcccggtc gtccagacgc cgggcctgtt cctctttcga gatatcgaac
     1561 cagaatttga tcagcaggat tccggaatcc gtcagcagcc gttcgaaccg cggcgcgtcc
     1621 ttcaggaact gttcgtgctg ctccggcgtg cagaagccca tcaccggctc gacacctgcg
     1681 cggttgtacc aggaccggtt gaagatcgac gtctcgccct ccgtcggcag gtgcgggaca
     1741 tagcgttgga aataccattg gccggtctcg cgctcggtcg gcttgggcag ggcgacgacc
     1801 cgggtctgac gcggcgacat atattccgtc agccgggtga tggacccgtc cttgcccgcg
     1861 gcgtcgcggc cttcgaacac catcacggtt ttctgaccct tctcgatggc ccaggcctgg
     1921 gcctcgacca gcttcagctg caggcgttcg agctcggtct ggtagtcgtc ggtgtttttt
     1981 cccatgggcc gaccttggcc cccgggctac cgcaggcaag atgaccgaat gttcagatgc
     2041 cgccgaacgg cgtccggagt aggagtcgcc gaattccagg aggcggcatg agcatcatcc
     2101 ggcgcacctt gctgagctgg gccgcgggcc tgccggcctt ggcggcgatg cccgccgtgg
     2161 cccgggccga aaccttcgat gaggcgctgg acgcggcctt tcgtgtcgg
//
LOCUS       DASVVN010000420         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_361493,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000420 DASVVN010000000
VERSION     DASVVN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361493"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF686_06725"
     CDS             complement(<1..498)
                     /locus_tag="VF686_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661543.1"
                     /translation="MASTLVADGPETRRSVERFSTHELGPTLERFRSSGMLEEGRINL
                     IGLDAIAERLGDKWAGRQEQIYDHVERTLTRHLEGGGFFQRVSPTDYLVVQPALAKFS
                     GQVSCLLYLRELLTHFLGGAAPDAVDVLEVTSLSAGRILAAEIDRAHAVKAAKTEPPV
                     VPPEDD"
     gene            complement(555..929)
                     /locus_tag="VF686_06730"
     CDS             complement(555..929)
                     /locus_tag="VF686_06730"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8661544.1"
                     /translation="MAKTDFRTVEDYFATRSEEERAVLLRMQDAILSAVPEAEPVISY
                     QIPAYKADGWLFYISAYTNHYSLASPPPFFEGFEDALSEYSVSKSAVQFPKDRPIPYA
                     LISEMAKTRARQNRERAARKKG"
     gene            complement(993..>1997)
                     /locus_tag="VF686_06735"
     CDS             complement(993..>1997)
                     /locus_tag="VF686_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX8661545.1"
                     /translation="GELACPFQRIEHLKHFVSRRAFDIEGLGEKQLIAFHERGWINQP
                     ADIFRLARDNEKLDALRAEDGYGETSVRNLVAGIDARRTIALDRFIYGLGVRDIGEQT
                     SIVLARAFETWSVFEAACLAAAEGIPSEDWVRLNEAHAVSPRVVATLAEAAPPAADPW
                     PEAPIDQKIALAFPGLAAPARRGLAALADDWAGLVRLAAVARNEGPSDGLGQIAGVSG
                     VGPVAARALALFFRETHNRAMVDALVAEMDRIEDAEKPRSDTAVAGKTVVFTGALERF
                     TRDEAKARAESLGAKVSGSVSKKTDYLVAGPGAGSKMADALKHGVKVLTEDEWLALIG
                     "
BASE COUNT          329 a          641 c          701 g          326 t
ORIGIN      
        1 gtcatcctcc ggtgggacga caggcggttc cgtcttcgca gccttgaccg catgggcgcg
       61 gtcgatctcg gcggcgagga tgcggcccgc gctcaggctg gtcacctcca gcacgtcgac
      121 ggcgtcgggc gcggcgccgc ccagaaaatg ggtcagcagc tcgcgtagat acagcagaca
      181 ggagacctgg ccgctgaact tggccaacgc cggctgcacc acgagatagt cggtcggcga
      241 cactcgctgg aagaagccgc cgccctcgag gtgacgggtc agggtgcgtt cgacgtgatc
      301 gtagatctgt tcctgacggc cggcccactt gtcgccgagg cgctcggcga tggcgtccag
      361 accgatcaga ttgatgcggc cttcctcgag catgcccgag gaccggaaac gctccagcgt
      421 gggtcccagc tcgtgggtgg agaaccgctc gacgctgcgg cgggtctccg gcccgtcagc
      481 gacaagagta ctggccatga agccattgaa acgcgacagg attatttatt ggtgaaatcg
      541 gcgaaaggcg gctcctagcc ctttttcctc gccgcccgct cgcggttctg ccgcgcccgg
      601 gtcttcgcca tctccgagat cagggcgtag gggatcgggc ggtccttcgg gaactgcacc
      661 gccgatttcg agaccgaata ttccgagagc gcatcctcga acccctcgaa gaagggcggc
      721 ggggaggcca ggctgtagtg attggtgtag gccgagatgt agaatagcca gccgtccgcc
      781 ttgtaggccg gtatctggta gctgatgacc ggctccgcct ccggcacagc ggagagaatg
      841 gcgtcctgca tcctcagcag cacggcccgc tcctcttccg agcgggtggc gaaatagtcc
      901 tcgacggtgc ggaaatcggt cttcgccatg acggcctcgc gatctgcatt taaccagaat
      961 gttaaataga ccgatccggc gccgcgatca agtcagccga tcagggccag ccattcgtcc
     1021 tcggtaagca ccttcacgcc gtgtttcagg gcgtcggcca tcttggagcc ggcaccgggg
     1081 ccggcgacca gatagtcggt ctttttcgac accgagccgg acaccttggc gccgaggctc
     1141 tcggcccggg ccttggcctc gtcgcgggtg aagcgttcca gggcgccggt gaagaccacg
     1201 gtcttgccgg cgacggcagt gtcggacctg ggcttttcgg cgtcctcgat gcggtccatt
     1261 tcggcgacca gggcgtcgac catggcgcgg ttgtgggtct cgcggaaaaa cagcgccagg
     1321 gcgcgggccg cgaccggacc gacgcccgag acgccggcga tctggcccag accatcggac
     1381 gggccctcgt tgcgggccac ggccgccagc cgcaccagac cggcccagtc gtcggccaag
     1441 gcagccagac cccggcgggc gggggcggcg aggccgggga aggcgagggc gatcttctgg
     1501 tcgatcgggg cctcgggcca gggatcggcg gcgggcggcg cggcctcggc cagggtcgcc
     1561 accacgcgcg gcgagacggc atgggcctcg ttcagccgga cccagtcctc gctcggaatg
     1621 ccctcggcgg cggccaggca ggcggcctcg aagacggacc aggtctcgaa ggcgcgggcc
     1681 aggacaatgg aggtctgttc gccgatgtcg cgcacgccga ggccgtagat gaaccggtcc
     1741 agcgcgatgg tccggcgggc gtcgatgccg gccacgaggt tgcgcacgct ggtctcgcca
     1801 tagccgtcct cggcgcgcag ggcgtccagc ttctcgttgt cgcgggcgag gcggaagatg
     1861 tcggcgggct ggttgatcca gccgcgctca tggaaagcga tcagctgttt ttcaccaaga
     1921 ccttcaatgt cgaaggcgcg acggctgacg aaatgcttca gatgttcgat gcgctggaac
     1981 gggcaggcca actctcc
//
LOCUS       DASVVN010000421         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_463207,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000421 DASVVN010000000
VERSION     DASVVN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463207"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2288
                     /locus_tag="VF686_06740"
     CDS             <1..2288
                     /locus_tag="VF686_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8661546.1"
                     /translation="IMLGVVMRERWNGTVLGEALAHEVAEIRAELPAGMTLTQTADQS
                     KNIAYAYNEFMLKFVVALGVVMVVSLVALGFRVGLVVAAAVPLTLSAVFVIMLMTGRD
                     FDRITLGALILALGLLVDDAIIAIEMMVVKMQEGMERTAAATFAWGATAAPMLTGTLV
                     TVIGFLPVGFAQSTAGEYAGNIFWIVAFALLVSWVVAVYFTPYLGVKLLPRIKVEEGG
                     HDALYATKRYERFRRWVRSAVANRGKVSIAVVAALMVAGGLMAVVVPKQFFPNSDRPE
                     LLIEVAMPKGTSIRATEALVARIETDVRRHEEAVYTDSYVGAGAPRFFLSANPELPDP
                     SFAKIVILTKGHEHRDELRQIIADRIASGAYPEARVRVSTLLFGPPVPYPVAFRISGP
                     DPDKLRQIAARTADIVRADPRTVGVNTDWGERAPSIRLEFDEARLRLLGLDPASVSQQ
                     VGALMSGATVSQMRTEDRTVNVVVRADPEDRRDLDGLDNLVISTADGRPVPLSGVARL
                     VPAFEDPILMRRDRQPTITVRADIVGHVQPPVVTAAILPRLEDLKASLPSGYAITAAG
                     SVEESAKANAALAPLFPLMIVLMLTVIMLQTRSFKMTGLVFATGPLGLIGAVPALLLT
                     GTPFGFNAILGLIGLAGILMRNTLILVDQIEAERARGLNQIDAVVEATVRRARPVLLT
                     ALAAVLAFLPLTLSTFWGALAVVLIGGTLVGTGLTLFFLPALYALWFTSRLPRPRAGR
                     GAASRRGPGAPDQAPAIEQGN"
BASE COUNT          344 a          820 c          786 g          434 t
ORIGIN      
        1 cgatcatgct gggcgtcgtc atgcgcgaac gctggaacgg caccgttctg ggcgaggcgc
       61 tggcccatga ggtcgccgaa atacgcgccg aactgccggc cggcatgacc ctgacgcaga
      121 cggccgacca gtcgaagaac atcgcctacg cctacaacga gttcatgctg aagttcgtgg
      181 tggccctggg cgtcgtcatg gtcgtcagcc tggtcgcgct cggcttccgg gtcggcctcg
      241 tcgtcgcggc cgccgtgccc ctgaccctgt cggccgtctt cgtcatcatg ttgatgacgg
      301 gccgggattt cgatcgcatc acgttgggcg cgttgatcct ggcgctcggc ctgctcgtcg
      361 acgacgccat catcgccatc gagatgatgg tggtgaagat gcaggagggg atggagcgca
      421 cggccgccgc aaccttcgcc tggggcgcga ccgcggcccc tatgctgacg ggcacgcttg
      481 tcaccgtgat cggcttcctg cccgtgggtt tcgctcagtc cacagcgggc gaatacgcgg
      541 gcaacatctt ctggatcgtc gccttcgccc tgctcgtgtc ctgggttgtg gctgtctatt
      601 tcaccccgta tctcggcgtg aaactgctgc ccaggatcaa ggtggaggag ggggggcacg
      661 acgccctcta cgccaccaaa cgctatgagc ggttccgccg ctgggtccgg agcgcagtcg
      721 ccaaccgggg caaggtctcg atcgctgtcg tcgccgcact gatggtcgca ggggggctga
      781 tggccgtggt cgtgcccaag cagttcttcc ccaactccga ccggcccgaa ctgctgatcg
      841 aggtggcgat gcccaagggc accagcatcc gcgcgacgga ggctctggtc gcccggatcg
      901 agacggatgt ccgccgtcac gaggaggctg tctataccga cagctacgtc ggcgcggggg
      961 caccccggtt cttcctgtcg gccaacccgg aactgccgga tccctccttc gccaagatcg
     1021 tcatactgac caaaggtcac gaacaccgcg acgaactgcg ccagatcatc gccgaccgga
     1081 tcgcgtccgg cgcctatccc gaggctcggg ttcgcgtctc gaccctgctg ttcggtcccc
     1141 ccgtgccata tccggtcgcc ttccggatct ccggtcccga tccggacaag ctgcgtcaga
     1201 tcgcggcccg gactgctgat atcgtgcgag cagacccgcg cactgtgggg gtgaatacgg
     1261 actggggcga gcgcgctccg agcatccgtc tggagtttga cgaggcgcgt ctgaggcttc
     1321 tgggcctcga ccccgccagc gtctcgcagc aggtcggcgc cctgatgtcc ggcgccaccg
     1381 tgtcccagat gcggaccgag gatcgcacgg tcaatgtcgt cgtccgggcc gatccggagg
     1441 atcgccgcga cctcgacggg ctcgacaacc tggtcatcag cacggccgac ggtcgtccgg
     1501 tcccgctcag cggcgtcgcc cgcctggtcc cggcgttcga ggatcccatc ctgatgcggc
     1561 gtgaccgtca gccgacgatc accgtgcgcg ctgacatcgt gggccacgtc cagccgccgg
     1621 tcgtgaccgc cgcgatcctg cccaggctcg aggacctcaa ggccagtctg ccgagcggct
     1681 atgcgatcac ggcggcgggc tcggtcgagg aatcggccaa ggccaacgcc gccctcgccc
     1741 cgctcttccc cttgatgatc gtgctgatgc tgaccgtcat catgttgcag acgcggtcgt
     1801 tcaagatgac ggggctggtg ttcgccacgg gaccgctcgg cctgatcggc gccgtgcccg
     1861 ccctgctgct caccggcacg ccgttcggct tcaacgccat cctgggtctg atcggtctgg
     1921 cgggcatcct gatgcgcaac accctgatcc tcgtcgatca gatcgaggcg gaacgagcgc
     1981 ggggcctcaa tcagatcgac gccgtggtcg aggcgacggt ccggcgcgcc cggccggtgc
     2041 tgctgaccgc cctggcggcg gtgctggcct tcctgccgct gacgctgtcc accttctggg
     2101 gcgcgctcgc cgtcgtgctg atcggcggaa cgctggtggg aacggggctc acgctgttct
     2161 tcctgccggc gctctacgcc ttgtggttca ccagccgttt gccccggccg agggccggca
     2221 gaggcgccgc ctcgaggcgg gggcccggtg cgcccgatca ggctcccgcc atcgaacaag
     2281 ggaattagtc catgcctgtg tccccactct cagagatcgg tcgaaacagc gagtgcccct
     2341 ggacgcgctt cctgaggttt cttcatgagc ttgatgacgc gttg
//
LOCUS       DASVVN010000422         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_200511,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000422 DASVVN010000000
VERSION     DASVVN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200511"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VF686_06745"
     CDS             <1..397
                     /locus_tag="VF686_06745"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8661547.1"
                     /translation="VIETLDRPRLADALAAAGGKAGKPVRVLVQVNTGAEPQKAGALP
                     GDADALIAHARSLGLIVEGLMCIPPADEAPDPHFTLMGEMARRNGLAVLSMGMSGDYE
                     TAIRLGATHVRVGSALFGERNTPQTPSMD"
     gene            460..1146
                     /locus_tag="VF686_06750"
     CDS             460..1146
                     /locus_tag="VF686_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8661548.1"
                     /translation="MPTPKTILIIDDDDDLREALAEQLALHEAFRPVQASTAAEGVRM
                     GREVRADLILLDVDLPDMDGREACRLLRKDGVSTPIIMLTGQSSDSDTVLGLESGAND
                     YVTKPFRFAVLLARIRAHLRSHEQSEDAVFRIGPYEFRPAAKVLVDDKARKVRLTEKE
                     TSILKYLYRAGAKAVSREELLTEVWGYNAGVTTHTLETHIYRLRQKIEPEPGQARLLL
                     TDAGGYRLQP"
     gene            complement(1302..2096)
                     /locus_tag="VF686_06755"
     CDS             complement(1302..2096)
                     /locus_tag="VF686_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8661549.1"
                     /translation="MLRIATWNINSVRLRIDQVARFVEESGTDVLCLQEIKCLEDQFP
                     RAAFEAMGMPHLKIGGQKGWHGVAIASRKPITAAPALDVCRERHARCCGVVIDGVEIQ
                     NFYIPAGGDTPDRALNPKFDHKMDFYERLTAEMAGRDKQRPLVLAGDFNIAPGENDVW
                     NHRYMSKIVSHTPLEVETLRRLQAAGGFADVVRDQIPEPQKLASWWSYRAADFRKSNR
                     GLRLDHIWTSPGLTPAVTPGTARIHDTVREWTQPSDHAPVTVDLAV"
BASE COUNT          390 a          822 c          851 g          388 t
ORIGIN      
        1 cgtcatcgag accctggatc ggccgaggct ggcggacgcc ctggccgcgg cgggcggaaa
       61 ggcgggcaaa ccggtgcggg tgctggtgca ggtcaacacc ggggccgaac cgcagaaggc
      121 cggagcgctg cccggggacg ccgacgccct gatcgcccat gcccggtcgc tgggactgat
      181 cgtcgagggc ctgatgtgca ttccgcccgc cgacgaagcg cccgatcccc atttcaccct
      241 gatgggcgag atggcccgcc gcaacggtct ggcggtgctg tcgatgggga tgagcggcga
      301 ctacgagacg gcgattcgcc tcggcgcgac ccatgtgcgc gtcggatcgg cgctattcgg
      361 ggaacgaaac accccccaaa cgccctctat ggactgaaaa ccgctccgct tgcggtccaa
      421 cccttggcgg aaccgccgac gctcgccatt ctggtatcca tgcccacgcc caagaccatc
      481 ctgatcatcg acgacgacga cgacctgcgc gaggcgctcg ccgagcagct ggccctgcac
      541 gaggccttcc ggccggtgca ggcctcgacc gccgccgagg gcgtgcggat ggggcgcgag
      601 gtgcgggccg acctgatcct gctggacgtc gacctgcccg acatggacgg ccgcgaggcc
      661 tgccgcctgc tgcgcaagga cggcgtctcg accccgatca tcatgctgac cggccagtcg
      721 tcggattccg acaccgtgct ggggctggag tcgggggcca acgactatgt caccaagccc
      781 ttccgcttcg ccgtcctgct ggcccgcatc cgggcccacc tgcgcagcca cgaacagtcc
      841 gaggacgcgg tcttccgcat cggcccctat gagttccggc ccgccgccaa ggtcctggtc
      901 gacgacaagg cgcgcaaggt gcggctgacc gagaaggaaa ccagcatcct gaaatacctc
      961 tatcgcgccg gggccaaggc cgtgtcgcgc gaggagctgc tgaccgaggt ctggggctac
     1021 aacgccgggg tcacgaccca tacgctggaa acccacatct atcgcctgcg ccagaagatc
     1081 gagcccgagc ccggtcaggc gcggctgctg ctgaccgacg cgggcggata ccggctgcag
     1141 ccgtagtcag tccggcgaaa aacaccgtcc gaagagtcca ctgagtccac ttcgggccgc
     1201 cgctgtttct gttgtcaaag accgccgccg ctgagtttga gcaccaaagc acgcgcacgc
     1261 gtcaaaaatg gtcgtgggcc gcgagacggg tccgattgta ttcagaccgc cagatcgacc
     1321 gtcaccggcg cgtggtcgct gggctgggtc cattcgcgga ccgtgtcgtg gatgcgggcc
     1381 gtgccgggcg tcaccgccgg ggtcaggccg ggcgacgtcc agatgtggtc gaggcgcagg
     1441 ccgcggttgg acttgcggaa gtcggcggcg cggtagctcc accagctggc cagtttctgc
     1501 ggctcgggga tctggtcgcg gaccacgtcg gcgaaaccgc cggcggcctg aaggcggcgc
     1561 agggtctcga cctccagcgg cgtgtggctg acaatcttcg acatgtagcg gtggttccag
     1621 acgtcgttct cgcccggcgc gatgttgaaa tcgccggcca gcaccagcgg gcgctgtttg
     1681 tcgcggcccg ccatctcggc ggtgagccgc tcgtagaagt ccatcttgtg atcgaatttc
     1741 gggttcagcg cccggtccgg cgtatcgccg ccggcgggga tgtagaaatt ctggatctcg
     1801 accccgtcga tcacgacccc gcagcagcgc gcgtggcgtt cacggcagac gtcgagcgcc
     1861 ggcgccgccg tgatcggctt gcggctggcg atggcgacgc cgtgccagcc cttctgaccg
     1921 ccgatcttca ggtggggcat gcccatggcc tcgaaagcgg cgcgcgggaa ctggtcctcg
     1981 aggcatttga tctcctgcag gcacaggacg tcggtcccgc tctcctcgac gaagcgggcg
     2041 acctgatcga tgcgcaggcg gacggagttg atgttccagg tggcgatgcg aagcatctgc
     2101 cggctctagc acctaagccg ggatcgttga tcccctgctg tcgtcattcc gggcaagccg
     2161 cccttgcggc gcgacccgga acccggcggc gcgcgagagc gcgaatctgt cgggagcgtc
     2221 gtcgcctgcg gagcgtaaag gcgtcggcgg tgtttcgccg ccgtcgcggc gccgggttcc
     2281 gggtctccgc tgcgcttcgc ccggaatgac gagtgagagg ggccggctct tcagggatcc
     2341 tctccaaaca aaaaagcgcc cgatcggggg gatcgggcgc tggaaggttc gtctctcgcc
     2401 gccgggaagg ggatgaaatc catagcggtc gggtcggctc ccgccgacct g
//
LOCUS       DASVVN010000423         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_68870, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000423 DASVVN010000000
VERSION     DASVVN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68870"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VF686_06760"
     CDS             <1..481
                     /locus_tag="VF686_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661550.1"
                     /translation="IAALAILVAAGKGPAEASIPPSYRVLVVSSDREALALSEALVQY
                     MSAAEAFFREPPAVAQSGVRACLQAPDFGACARPLIPRPEHWREAHHVIIRASQNGPT
                     GLAWTCVGSGAHRPPTAEQTTVIDVQPALFGEDEEQSSQLRVAMNCIQSAAAESVAP"
     gene            complement(516..851)
                     /locus_tag="VF686_06765"
     CDS             complement(516..851)
                     /locus_tag="VF686_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661551.1"
                     /translation="MIILGLTLLAFGAQALPGTPCPEVGYPQDYWGSIRNNITSGDFT
                     LQTQEGITDCRFTSVGIGRCFVYGPARFLVKSKGRNTYFAIPDRLGADIQISEGSFTC
                     RARPFVGTD"
     gene            complement(1011..1310)
                     /gene="rpmB"
                     /locus_tag="VF686_06770"
     CDS             complement(1011..1310)
                     /gene="rpmB"
                     /locus_tag="VF686_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8661552.1"
                     /translation="MSRRCELTGIGPMVGHSVSHSNVKTKRRFLPSLKTVKVASEALG
                     QSFSLRISNAALRTLDFKGGLDPFIVKAKDEQLSTAAQRIKRQVKAKLAEQAAAA"
     gene            1529..1999
                     /locus_tag="VF686_06775"
     CDS             1529..1999
                     /locus_tag="VF686_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX8661553.1"
                     /translation="MKMSVLLRTAPVLIGALLMAGAPMAGPAAPVQSATPPVLPGYWQ
                     YTTSAVGQRDTETKCVRPSEINRFFGGLSTRHYRCTYPTREVGNGNARFEGNCVSRSG
                     RQVRVRLNGTYQPEQFSFRGGASIKAAGVWSPYIPATITARRLAAQCPANAEYF"
     gene            complement(2018..2311)
                     /locus_tag="VF686_06780"
     CDS             complement(2018..2311)
                     /locus_tag="VF686_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661554.1"
                     /translation="MSRPLQDKDDQWRGERPNGAVPIFDPGMSPLGTDDEAGGAPSTV
                     RTPDPDSWRREPLPTSPDRNGAGLRLTPRVWYAAAVAVVILLAAAGWLSLQAG"
     gene            complement(2308..>2524)
                     /locus_tag="VF686_06785"
     CDS             complement(2308..>2524)
                     /locus_tag="VF686_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661555.1"
                     /translation="EPGEVVAAVAAVDGRLVRVVGEGSIVVLRLPESARPWTLYRHGA
                     WLVSGTAPAGCLNWSRTVETSGKVQNT"
BASE COUNT          420 a          877 c          811 g          416 t
ORIGIN      
        1 catcgccgcc ctggcgatcc tcgtcgccgc ggggaagggc cccgcagagg cttccatacc
       61 tccgtcgtat cgcgtgctcg tcgtttcgag cgaccgggag gcgctggccc tgtcggaggc
      121 cctcgtccag tacatgagcg cggccgaggc cttcttccgc gagccgcctg cggtggctca
      181 atccggagtc cgggcgtgtt tgcaagcccc cgatttcggc gcttgcgcgc ggcccctcat
      241 accccggccc gagcactgga gagaggcgca ccatgtgatc atccgggcgt cgcagaacgg
      301 tccaacaggc ctggcctgga catgcgtcgg ctcaggcgca catcgccccc cgacggcgga
      361 gcagacgacc gtgatcgacg tccagccggc gctattcggc gaggatgagg aacagagcag
      421 ccagctgaga gtcgcgatga actgcataca gtcggccgct gcggagtccg tcgcgccctg
      481 aaccaccccg cggtcgtaca tcgagccagg cgaagctagt cagttcccac aaagggcctt
      541 gcacgacagg tgaacgatcc ttcgctgatc tggatatccg cgccgagacg atcgggaatc
      601 gcgaagtagg tgttcctgcc tttcgacttc accagaaatc tcgcaggccc atacacgaag
      661 caccgtccaa tcccgacact ggtgaaccgg cagtcggtga tgccttcctg cgtctgcagc
      721 gtgaagtctc cggacgtgat gttattccgg atcgagcccc agtaatcctg cggatagcca
      781 acctccgggc agggcgtgcc gggtagcgcc tgcgctccga acgccaacaa cgtaagccca
      841 aggatgatca tgcctgtgcc ctcagttcgc tggagagtac ccgctggcgg atagtgcgca
      901 acgggtcggg ggagcggtgc ttgatgcaag accggtcggc cgggtacccg ccaaaagaaa
      961 aaggccggtg tttccaccgg cctcttcgaa tcttcagcgg cgtcgccgga tcaggcggcg
     1021 gcggcttgct cggccagctt ggccttcacc tggcgcttga tgcgctgggc ggcggtcgag
     1081 agctgctcgt ccttggcctt gacgatgaac gggtccagcc cgcccttgaa gtccagggtg
     1141 cgcagggcgg cgttcgagat gcgcaggctg aacgactggc ccagcgcttc cgaggcgacc
     1201 ttgaccgtct tcagcgacgg caggaagcgg cgcttggtct tgacgttcga gtggctcacg
     1261 ctgtggccga ccatggggcc gataccggtg agttcgcagc gacgcgacat ctgactatcc
     1321 ttgacggagc ggtccacgaa acggcgcgga cgcatgaaac aaaggcgcgc agggatcggg
     1381 tccccgcgcg agagggggcc gtatagtgga agtcaccgcg ccgcgtcaag gcttgtagcg
     1441 gtcttcgacg cgttcagccg ccgttcaatt ccggccatat atcgcttcta tccattgcag
     1501 cgaagctccg ccgccgaagg tccgttcgat gaagatgtct gtcctgctcc gcaccgcccc
     1561 cgtcctgatc ggcgccctgc tgatggccgg cgcccccatg gccggcccgg ccgcgccggt
     1621 tcaatcggcc acgcctcccg tcctgcccgg ctattggcag tacaccacca gcgcggtggg
     1681 ccaacgggac accgagacca aatgcgtacg gcccagcgag atcaaccgct tcttcggcgg
     1741 cctgtcgacg cgccactacc gctgcaccta tccgacccgc gaggtcggca atggcaacgc
     1801 ccgcttcgag ggcaactgcg tctcgcgcag cggccgtcag gtgcgcgtgc gtctgaacgg
     1861 cacctatcag cccgagcagt tcagcttccg cggcggcgcc tcgatcaagg ccgccggcgt
     1921 ctggagccct tacattccgg cgacgatcac cgcccgccgt ctggcggcgc agtgcccggc
     1981 gaacgcggaa tatttctagg cgcacggcgc cggccgtcta gccggcctgc agtgagagcc
     2041 agcccgcggc cgcgagcagg atcacgacgg caacagccgc cgcgtaccag accctggggg
     2101 tcaacctcag cccggcgccg ttgcgatcgg gcgaagtcgg gagcggttcc cgcctccagc
     2161 tgtccggatc cggcgttcgg accgtggacg gcgctccccc cgcttcgtca tcggtcccca
     2221 gcggcgacat gcccggatcg aagatcggga ccgcgccgtt agggcgctcg ccgcgccact
     2281 gatcgtcctt gtcctgcagt ggacggctca tgtgttttgc accttcccgg aggtttcaac
     2341 cgttcgcgac cagttcagac atcctgcggg ggccgtcccg ctgaccagcc aggcgccgtg
     2401 tcgatacagg gtccacggcc gcgcggattc aggcaggcgc aggaccacga tcgatccttc
     2461 cccgacaacc ctgaccagac ggccatcaac cgccgctacg gcggccacca cctcgcccgg
     2521 ctcg
//
LOCUS       DASVVN010000424         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_410602,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000424 DASVVN010000000
VERSION     DASVVN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410602"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /gene="recQ"
                     /locus_tag="VF686_06790"
     CDS             complement(<1..1625)
                     /gene="recQ"
                     /locus_tag="VF686_06790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX8661556.1"
                     /translation="MLADSTLTSIAPPSLADARATLERVWGHGDFRGLQADVVGEILA
                     GRDVLAVLPTGGGKSVCYQIPAMLRPGLGLVVSPLIALMTDQVEALKQQGVAAARLDS
                     GVSMDDRSAIWRQARAGELDLLYVSPEGLAQPHLIDRLHDLPLSLIAIDEAHCVSQWG
                     HDFRPDYRTLGRLAELFPGVPRIAVTATADARTREDIVRSLHLEKAKVLVDSFARPNL
                     QLSAVRKENASRAKTDLAVIDLVRQRRGKSGVVYCGSRDGCERVADALKAAGTNAIAY
                     HAGMDARDRDRRLERFLAEDGAVMVATIAFGMGVDKADVRFVIHADPPGSLEAYWQEI
                     GRAGRDGEPAEGITLYGPSDIAWTLKRLDGRPMAEEVKAAQVRKARQLFAMLDGAACR
                     PQAVRRYFGEAEAQPCGVCDLCADPPAVFDATVPAQKALAAVQRLGERFGKTRVIDHL
                     LGRTKDVQPWETALSTWGIGADTAVHTWRDVLDHLMFDGLLAEDPNDGKPLVRLGDPE
                     AVRAVYRGERSVEVRSLPARVGSGVAKERVRTGR"
     gene            2370..3269
                     /locus_tag="VF686_06795"
     CDS             2370..3269
                     /locus_tag="VF686_06795"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8661557.1"
                     /translation="MTTLLYGRPPAVFDPPGSATQVSPLIPGSTPLEDVAEGSADEVM
                     IYAPPGVLERRHTLALALRALKPGGRLDAMAPKDKGGSRLKKELEGFGVKVGESAKAH
                     HRRCVVIRPETLTGIDEAIAAGAPRLVEGLEAWSQPGIFAWDRIDAGSLLLAHTAPAL
                     KGAGADLGCGYGALATVVLGSPAVTSLRLIDLDRRAVTAARKNVTDPRASFEWADARN
                     VEAAGDLDFVVTNPPFHDGGAEDKRLGQAFIRQAAGMLGKGGVLWLVANRHLPYEAEL
                     NAAFKRVRMAVDAGGYKVFEAVK"
     gene            3308..3997
                     /locus_tag="VF686_06800"
     CDS             3308..3997
                     /locus_tag="VF686_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8661558.1"
                     /translation="MGYGSRTEMARLGKAGGIVLDGADLTDVSKRIAVTPDLPSRMEI
                     DGEPLDPPPGLVMMLNKPLGMTCSRKEDGALVYDVLPERWRRRDPAISTIGRLDKQTT
                     GLLLLTDDGDLLHRVISPKRHVAKVYRATLARPLDGTEGDVFAAGGLVLEGEDKPLSP
                     AVLEMLSPTEARLTVTEGRYHMVRRMFAAVGNHVEGLHRERVGGLALPDDLAPGDWRL
                     LDQAQIDLIFA"
BASE COUNT          613 a         1371 c         1580 g          634 t
ORIGIN      
        1 cggccggtgc ggacgcgctc tttcgccact cccgagccga cgcgcgcggg aaggctgcgg
       61 acctcgacgg accgttcgcc gcgatagacg gcgcgcacgg cctcgggatc gcccagccgc
      121 accagcggct tgccgtcgtt gggatcctcg gccagcaggc cgtcgaacat caggtggtcg
      181 agcacgtcgc gccatgtgtg caccgccgtg tcggcgccga tgccccaggt ggacagggcc
      241 gtctcccacg gctggacgtc cttcgtccgg ccgagcaggt ggtcgatgac ccgggtcttg
      301 ccgaagcgct cgccgagacg ctgcaccgcc gccagcgcct tctgggccgg gacggtggcg
      361 tcgaacacgg ccgggggatc ggcgcacagg tcgcagaccc cgcacggctg cgcctcggcc
      421 tcgccgaaat agcggcgcac ggcctggggc cggcaggccg cgccgtccag catggcgaac
      481 aactgccgcg ccttgcggac ctgcgccgcc ttgacctcct cggccatggg tcgtccgtcg
      541 agccgcttca gcgtccaggc gatgtctgac ggaccataga gagtgatgcc ctcggccggt
      601 tcgccgtcgc ggccggcgcg gccgatctcc tgccagtagg cttccagcga gcccggcgga
      661 tcggcgtgga tgacgaaacg gacgtcggcc ttgtcgaccc ccatgccgaa ggcgatggtc
      721 gcgaccatga cggcgccgtc ctcggcgagg aagcgctcga gccggcggtc cctgtcccgg
      781 gcgtccatgc cggcgtgata ggcgatggcg ttggtcccgg cggccttcag ggcgtcggcg
      841 acgcgctcgc agccgtcgcg cgagccgcaa tagacgacgc cggacttgcc gcgccgctga
      901 cgcaccagat cgatgacggc gaggtccgtc ttcgcccgcg aggcgttctc cttgcgcacg
      961 gccgagagct gcaggttcgg gcgggcgaaa ctgtcgacga ggaccttggc cttctccaga
     1021 tgcagggagc ggacgatgtc ctcgcgggtg cgggcgtcgg cggtggcggt gacggcgatg
     1081 cgcggcacgc cggggaagag ttcggccagg cggccgaggg tgcggtagtc ggggcggaag
     1141 tcgtggcccc attgcgaaac gcagtgcgcc tcgtcgatgg cgatcaggct caggggcagg
     1201 tcgtgcaacc ggtcgatcag gtgcggctgg gccagccctt cgggcgagac gtacagcagg
     1261 tccagttcgc cggcgcgggc ttgccgccag atggccgagc ggtcgtccat ggagacgccg
     1321 gagtcgagac gggcggcggc gacgccctgc tgtttcagcg cctcgacctg gtcggtcatc
     1381 agggcgatca ggggcgagac gacgaggccg aggccgggcc gcagcatggc cgggatctgg
     1441 taacagaccg acttgccgcc gccggtcggc agcaccgcca gcacgtcgcg gccggcgagg
     1501 atttcgccga caacgtcggc ctgcagcccc cggaaatcgc cgtggcccca gacccgctcc
     1561 agcgtcgcgc gggcgtcggc gagcgacggc ggcgcaattg acgttagagt tgaatcggcg
     1621 agcatcgtgc gcttgtgcct catttgttct cgtttgtcag tgaggaattg ggcatgcgtc
     1681 atcgctcttc ggcgtttccg cggaaaagtt cggggagggc gcggggcgac cgggctcgcc
     1741 cggtgctgtt ttggttttac cgtttcgacg agacagccgc cccgcgtgtt cgttttccac
     1801 gcgcgtgccg gccgccggcc gttctctggc cttaccggcg cccggggagg tccttgcgaa
     1861 ccccgtccgg ggggagactt gcgggcctgt agcccacaga ggtcccgcac aagaagcgcg
     1921 cggcgagcag gaagacgcca tccatcgacg tcctcaagga cccccggcgt aagcgccccc
     1981 gggcttcatc gctcgaccac agcccgccga catcgagaag cgtggatccg acgccctccc
     2041 caccgatggg atgggaggaa tgataaggcg gctggagcgg ctgctatgaa gagagttcgg
     2101 ggaaaacggg agtgtttgaa gaggcgggat attttctcgc caatgcgact atagaaacgc
     2161 cccactgatc cttctcccgt tgggagaagg tggcgcgcag cgccggatga gggtcgggcg
     2221 gcggacgcgg cgaagccgcc ccgggacagg cgaccctcac cctttcgcct tgccgatcgc
     2281 ttcgctcccg gaggctcaag ccctctccca gcgggagagg ggcaggcttg agtttcccgc
     2341 cgcgccgcat tcccgctata gcccgcgcca tgaccaccct tctgtatggc cgcccgccgg
     2401 cggtgttcga tccgcccggc tcggcgacgc aggtttcgcc gctgattccc ggctcgacgc
     2461 cactcgagga cgtggccgag ggctcggccg acgaggtcat gatctacgcc ccgcccggcg
     2521 tgctggagcg gcgccatacg ctggcgctgg cgctgcgggc gctgaagcca ggcggccggc
     2581 tggacgccat ggcccccaag gacaagggcg ggtcgcggct gaagaaggag ctggaaggct
     2641 tcggcgtcaa ggtcggcgaa agcgccaagg cgcaccaccg gcgctgcgtg gtcatccggc
     2701 cggagacgct gaccgggatc gacgaggcca tcgccgcggg cgcgccgcgt ctggtcgagg
     2761 ggctggaggc ctggtcgcag ccggggatat tcgcctggga ccggatcgac gcgggttcgc
     2821 tgctgctggc gcatacagcg ccggcgctga agggggcggg ggccgatctg ggctgcggct
     2881 acggcgcgct ggcgacggtg gtgctgggct cgccggcggt gacctcgctg cggctgatcg
     2941 atctggaccg gcgcgccgtc acggcggcga ggaagaatgt caccgatccg cgcgcgagct
     3001 tcgaatgggc ggatgcgcgg aacgtcgagg cggcgggcga tctggacttc gtggtgacca
     3061 atccgccgtt ccacgacggc ggggccgagg acaagcggct gggccaggcc ttcatccgtc
     3121 aggcggcggg catgctgggc aagggcgggg tgctttggct cgtggccaac cgccacctgc
     3181 cctatgaggc cgagttgaac gcggccttca agcgcgtccg gatggccgtc gacgcgggcg
     3241 ggtacaaggt gttcgaggcg gtgaagtgaa gacccccacc tcgcgcgtcg acaagctgct
     3301 gggctcgatg ggctatggct ctcgcacgga gatggcgcgg ctgggcaagg cgggcgggat
     3361 cgtgctggac ggggccgatc tgaccgatgt gtcgaagcgg atcgcggtga cgccggacct
     3421 gccgtcgcgg atggagatcg acggggagcc gctggatccg ccgccgggtc tggtgatgat
     3481 gctgaacaag ccgctgggca tgacctgctc gcgcaaggag gacggggccc tggtctatga
     3541 cgtcctgccc gaacgctggc ggcggcgcga cccggccatc tcgaccatag ggcggctgga
     3601 caagcagacc acgggcctgc tgctgctgac ggacgacggc gatctgctgc accgggtgat
     3661 ctcgcccaaa cggcacgtgg ccaaggtcta tcgcgcgacc ctggcgcggc cgctggacgg
     3721 gaccgagggc gacgtcttcg ccgccggcgg tctggtgctc gagggcgagg acaagccgct
     3781 gtcgccggcg gtgctggaga tgctgtcgcc gacagaggcg cggctgacgg ttaccgaagg
     3841 gcgctatcat atggtgcggc ggatgttcgc ggcggtgggc aatcacgtcg agggcctgca
     3901 ccgggaacgg gtcggcggcc tggccttgcc cgacgatctg gcgccgggcg actggcggct
     3961 gctggatcag gcgcagatcg atctgatttt cgcctgagct cctttcaccg tgcacctcgg
     4021 cgaatgccgg ggtccagatg acagtcagac tgccggaatg caggcctccg ggtccgcgcg
     4081 ccgtgtgtgg atctggatcc cggcattcgc cggcatgcac ggagagtggg agctgccggc
     4141 cttgacgctg gcggccgacg ggggcacctg cggcgcatga gcgcccacgc caagatct
//
LOCUS       DASVVN010000425         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_304189,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000425 DASVVN010000000
VERSION     DASVVN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304189"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF686_06805"
     CDS             <1..223
                     /locus_tag="VF686_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX8661559.1"
                     /translation="PKTYSVYVDETDKVWLTDFAANAIVRFDPTTETFQSFPSDRPGA
                     NVRQMLGRPGEVWGAESGTDRLVVVRHGD"
     gene            227..601
                     /locus_tag="VF686_06810"
     CDS             227..601
                     /locus_tag="VF686_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8661560.1"
                     /translation="MTTLWLLLAVLAAPPPPQADAGARAFQRCAACHALRSDDEAAPR
                     LGDVFGRRAGAIPGFAYSDAMKAAGRRGVVWNAPTLERFLADPDAVVPGTTMPYQGGS
                     AAERTALIDWLRRSNEPSRNRL"
     gene            672..>2365
                     /locus_tag="VF686_06815"
     CDS             672..>2365
                     /locus_tag="VF686_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8661561.1"
                     /translation="MRHAPFPAPTRRALLAGGAMAAGMVMAPRIAAAQAAADLDRVNA
                     LIARMTIEEKAGQLNLLNDPFRWRPEGVNPGDFNPDQASIAADIRAGRIGALFNGVGA
                     ATTRMVQDMAVRDSRLGIPLLFAADIVHGLRTTFPVPLGETASWDTDLAMRTARAAAV
                     EGGAAGVHQTYAPMVDIGRDQRWGRVVEGAGEDVLLGNLFAAARTRGFQGDDLKTWSS
                     LLACPKHFAAYGAAEAGMDYNSTDMSELELRSVYLPPFKAAFDAGALSTMSAFNDLNG
                     VPASGNRWLLTDILRGEWGFAGFVVSDYTSEQELIAHGYAADGRDAARLALMAGVDMS
                     MKSGLYLKHIPELVQSGDVPLARVDEAVRRVLMTKAALGLFENPYRGSDVGRERRVHG
                     SREHLALAREAGRKSIVLLKNEGDLLPLPKTGRRYALIGPFGADTTDLFGPWSIFKDP
                     KLAVSLEQGLRAALGEGSSLTVVKGSDVEAPIAGGIDAAVLAASQADVVLLAVGESGE
                     MSAESKSRVDIGVPEPQLALARAVAATGKPVAVLLRNGRGMELAGAIKDAPGILVT"
BASE COUNT          343 a          813 c          856 g          353 t
ORIGIN      
        1 cccgaaaacc tattcggtct atgtcgacga gacggacaag gtctggctga ccgatttcgc
       61 ggccaacgcc attgtccgct tcgacccgac gaccgagaca ttccagagct tcccgagcga
      121 tcggccgggg gccaatgtgc gccagatgct gggccgtccc ggagaggtct ggggcgcgga
      181 gtccggcacg gaccggctgg tcgtcgtccg gcacggcgac tgaggcgtga cgacactctg
      241 gctgctgctc gcggtcctcg ccgccccgcc cccgccacag gccgacgcag gcgcacgcgc
      301 cttccagcga tgtgcggcct gtcatgccct gcggtccgac gatgaggcgg cgcctcggct
      361 gggcgatgtg ttcggacggc gcgccggcgc catcccgggt ttcgcctatt cggacgccat
      421 gaaggcggcg ggccgacggg gtgtggtgtg gaacgccccc accctcgaac gctttctggc
      481 ggatcctgac gccgtcgtgc ccggcaccac catgccctat cagggcggtt cggccgcaga
      541 gcgaacggcg ctgatcgact ggttgcgccg atcgaacgag ccctcgcgca accggttgtg
      601 atcgccgcag gctaggcgcg gaccggcccg gccgttaggt ctggccttcc agccagtgga
      661 accggttaca catgcgtcac gcccccttcc ccgccccgac ccgacgcgcg cttctggccg
      721 gaggcgcgat ggcggccgga atggtgatgg cgccgcgcat cgcggcggcg caggcggcgg
      781 cggatctgga ccgggtcaac gccctgatcg cgcgcatgac gatcgaggag aaggccggcc
      841 agctgaacct gctgaacgac ccgttccggt ggcggcccga gggcgtgaac cccggcgact
      901 tcaatcccga tcaggcgagc atcgccgccg acatccgcgc cggccggatc ggcgccctgt
      961 tcaatggcgt gggcgcggcg acaacccgca tggtgcagga catggccgtc cgcgacagcc
     1021 ggctgggcat tcctctgctg ttcgccgccg acatcgtcca cggcctgcgc accaccttcc
     1081 ccgtgccgct gggcgagacc gccagctggg atacggatct ggccatgcgc accgcccggg
     1141 cggcggccgt cgagggcggg gccgcggggg tgcaccagac ctatgccccc atggtcgaca
     1201 tcggccgcga ccagcgctgg ggccgggtgg tcgagggcgc gggcgaggac gtgctgctgg
     1261 gcaatctgtt cgccgccgcc cgcacgcgcg gcttccaggg cgacgatctg aagacctggt
     1321 cgtcgctgtt ggcctgtccc aagcatttcg ccgcctatgg agccgccgag gcgggcatgg
     1381 actacaattc gaccgacatg agcgagctgg agctgcggtc ggtctatctg ccgccgttca
     1441 aggccgcctt cgacgccggc gccctctcga cgatgagcgc tttcaacgac ctgaacggcg
     1501 tgccggccag cggcaacaga tggctgctca ccgacatcct gcgcggcgag tggggcttcg
     1561 ccggcttcgt ggtctcggac tacacctcgg agcaggagct gatcgcccac ggctacgccg
     1621 ccgacggacg cgacgcggcg cggctggccc tcatggccgg cgtcgacatg tcgatgaaga
     1681 gcggcctgta cctcaaacac atccccgaac tggtccagtc cggcgacgtg cccttggcgc
     1741 gcgttgacga ggcggtgcgg cgggtgctga tgaccaaggc ggcgctgggt ctgttcgaaa
     1801 acccctaccg cggctcggac gtgggtaggg aacgccgcgt ccacggcagc cgcgaacatc
     1861 tggcgctggc gcgcgaggcc ggccgcaagt cgatcgtgct gctgaagaac gagggcgacc
     1921 tgctgcccct gccgaagacg ggcagacgct acgccctgat cggaccgttc ggggcggata
     1981 cgaccgacct gttcggccca tggtcgatct tcaaggatcc gaagctggcg gtgtcgctgg
     2041 aacaggggct gcgcgcggcg ctgggcgagg ggtccagtct gacggtggtc aagggttcgg
     2101 acgtcgaggc gccgatcgcc ggcgggatcg acgccgccgt gctcgccgcc tcgcaggccg
     2161 atgtggtgct gctggccgtc ggcgagtccg gcgagatgtc ggcggagtcc aagtcccgcg
     2221 tcgacatcgg cgtgccggaa ccgcaactgg cgctggcccg ggcggtggcc gcgacgggca
     2281 agccggtggc cgttctgctg cgcaacgggc gcggcatgga gctggccggc gcgatcaagg
     2341 acgcgccagg cattctggtc acgtg
//
LOCUS       DASVVN010000426         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_437590,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000426 DASVVN010000000
VERSION     DASVVN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437590"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VF686_06820"
     CDS             <1..150
                     /locus_tag="VF686_06820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505300.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEX8661562.1"
                     /translation="EAQATAEVKAAAADLAARAAEQILAARLAGQKSDPLLDAAIAQI
                     GSRLN"
     gene            complement(209..694)
                     /locus_tag="VF686_06825"
     CDS             complement(209..694)
                     /locus_tag="VF686_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX8661563.1"
                     /translation="MSRVQKGVVAGLAATVAVSVLEIANMMLGPWAASFPRLLSVMLQ
                     MPDTIAVGWAAHFFVGALVLGPAFGALCPRLPTDTPESKGIIFAIGAFILMGLTVAPL
                     VGALGGRPVGLFFMQGGFGTFAWMIATHAVYGIVLGNVYGRLVERDKRVRAMPGAAPA
                     H"
     gene            complement(811..>1806)
                     /gene="gcvPB"
                     /locus_tag="VF686_06830"
     CDS             complement(811..>1806)
                     /gene="gcvPB"
                     /locus_tag="VF686_06830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8661564.1"
                     /translation="TSAHGTNPATAAFVGYTVVEVAQTEDGRVDVADLAAKLGDDVAA
                     IMVTNPNTCGLFERDILEISRLTHEAGAYFYCDGANFNAIVGRVRPGDLGVDAMHINL
                     HKTFSTPHGGGGPGAGPVVLSEALAPFAPAPWVVHDADGYRLIEREEDEAAQAFGRLC
                     AFQGQMGMYTRALSYMMSHGSDGLRQVAEDAVLNANYIKARLSDLMSPAFPDGPCMHE
                     ALFDDEWLKGTEVTTLDFAKAMIDEGFHPMTMYFPLVVHGAMLIEPTETESKQELDRF
                     IEAMRALATAAKAGDVDRFKGAPFHAPLKRLDETRAARSPVLRWTAPEGSNIAAE"
BASE COUNT          304 a          633 c          590 g          279 t
ORIGIN      
        1 gaggcccagg ctaccgccga ggtgaaggcc gccgccgccg acctggccgc ccgcgccgcc
       61 gaacagatcc tggccgcgcg tctggccggt cagaaatccg acccgctgct cgacgcggcc
      121 atcgcccaga tcggctcgcg cctcaactag gcgtcgctgc agcccaaacg aacagcggcc
      181 ccgcctgtcc ggcggggccg ttttcgtttc agtgcgccgg cgccgccccg ggcatcgccc
      241 ggacccgctt gtcgcgctcg accagccgtc cgtaaacgtt gccgagcacg atgccgtaga
      301 ccgcatgggt cgcgatcatc caggcgaagg ttccgaaccc gccctgcatg aagaacaacc
      361 cgaccggccg tccgcccagg gctccgacca gcggcgccac cgtcagcccc atcaggatga
      421 aggccccgat ggcgaagatg atgcccttgg actccggcgt atccgtcggc agccgcggac
      481 acagcgcccc gaacgccggc cccagaacca gggctccgac gaagaagtgc gccgcccagc
      541 cgaccgcgat cgtgtcgggc atctgcagca tgacggaaag aagtctcggg aagctggcgg
      601 cccaggggcc cagcatcatg ttcgcgattt cgagcactga aaccgcgacg gtggccgcga
      661 gaccggcgac tacacccttc tgtacgcgtg acatcataac cgttagcgcc tcccaaacgc
      721 ttgttgtggc tcagactacg catgggagag catacgcctg ctgtgacagg cggcgttcac
      781 attgccgtgc ttcgcaaatg cgaaggacga ttactctgcg gcgatgttcg aaccttcggg
      841 ggcggtccag cgcagcaccg gcgagcgggc ggcgcgggtc tcgtccagcc gcttcagcgg
      901 cgcatggaac ggggcgccct tgaagcggtc gacgtcgccg gccttggccg ccgtcgccag
      961 cgcccgcatc gcctcgatga agcgatccag ctcctgcttc gactcggtct cggtcggctc
     1021 gatcagcatg gctccatgga cgaccagcgg gaaatacatg gtcatcgggt ggaagccctc
     1081 gtcgatcatc gccttggcga agtcgagcgt ggtcacctcc gtgcccttca gccattcatc
     1141 gtcgaacaga gcctcgtgca tgcacggacc gtcggggaag gcggggctca tcaggtccga
     1201 cagccgggcc ttgatatagt tcgcgttcag caccgcatcc tcggccacct gccgcaggcc
     1261 gtccgaaccg tggctcatca tgtagctgag ggcgcgggtg tacatcccca tctggccttg
     1321 gaaggcgcac aggcggccga aggcctgggc ggcctcgtct tcctcgcgct cgatcaggcg
     1381 gtagccgtcg gcgtcgtgca ccacccacgg ggctggcgcg aacggcgcca aggcctccga
     1441 cagcaccacc ggacccgcgc ctggcccgcc gccgccatgc ggcgtcgaga aggtcttgtg
     1501 caggttgata tgcatcgcat cgacgcccag atcgcccgga cgcacccggc cgacgatggc
     1561 gttgaaatta gcgccgtcgc agtagaaata ggcgccggcc tcatgcgtca ggcggctgat
     1621 ctcgaggatg tcgcgctcga acaggccgca ggtgttcgga ttggtgacca tgatggcggc
     1681 gacgtcgtcg cccagcttgg cggccagatc cgccacatcg acgcggccgt cctcggtctg
     1741 ggcgacttcc acgaccgtat agccgacgaa ggcggcggtg gccgggttcg tcccgtgggc
     1801 cgaggt
//
LOCUS       DASVVN010000427         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_386751,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000427 DASVVN010000000
VERSION     DASVVN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386751"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VF686_06835"
     CDS             <1..1318
                     /locus_tag="VF686_06835"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX8661565.1"
                     /translation="TDYPDMAALGRVEDELRALPPLVFAGEARHLTAKLAQVEAGNAF
                     LLQGGDCAESFKEFSTDNIRDTFRLILQMAVVLTFAGGKPVVKVGRIAGQFAKPRSSS
                     LETIGDETLPSYRGDIINGMAFTPEERLPDPQRLLRAYNQSASTLNLLRAFAGGGYAD
                     LYNIHRWTMGFAADSAYGHKYRELADKITEALTFMEAVGVTPESHPDLSRVEVFTSHE
                     ALLLNLESALTRLDGATGEWFDTSAHMVWIGERTRQLDGAHIEFARGIKNPVGLKCGP
                     TMEPDDLLPLIDALNPDNTPGRLTLIGRFGADKVGDRLPRLMKAVKAEGRRVVWSIDP
                     MHGNTLKAGNGYKTRPFERILAEVRGFIDVAEAEGVHPGGVHLEMTGQNVTECLGGAQ
                     ALTEDDLSSRYHTHCDPRLNADQSLELAFLVAERLKSGRRSQSEAA"
     gene            1320..1901
                     /locus_tag="VF686_06840"
     CDS             1320..1901
                     /locus_tag="VF686_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX8661566.1"
                     /translation="MDGVGRPAGDDVGGRCAYQGAPGAFSHEACLDLRPWDEAVAYDS
                     FEDAIAALKTGEVGCALIPVENTTIGRVEPAASLVEAAGLTVVSEIWRPIRLALMAPP
                     GATLRSVKTAESHPVALRQCPESLAALGLTPVEAFDTAGAARAVAEAADPTRAAVAPT
                     RAAEVYELSILRNDIQDSEDNRTRFVLLSANPG"
     gene            2132..>2752
                     /locus_tag="VF686_06845"
     CDS             2132..>2752
                     /locus_tag="VF686_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX8661567.1"
                     /translation="MPHSSTKPALQQVWPSPTGAPMTDISPEHMALAALRHEIDHLDD
                     ELLALFERRLAIAARVGQAKDAPAGPHVKLRPDREQAVLARVLAKAGPDNREAVESLW
                     RQIVGWGLARQGRIKVQVWSPIEPTRAFDGARLRFGAAADMRAVRDPEAALAWAAEGH
                     GVAVLAVNAESAWWIGLRRDWSMLSVFDGFGGATPTALAVGKIDPAA"
BASE COUNT          436 a         1008 c          901 g          407 t
ORIGIN      
        1 gacggactat cccgacatgg ccgctctggg ccgggtcgag gacgagcttc gggcgcttcc
       61 gccgctggtc ttcgcgggcg aggcccggca cctgaccgcc aaactggccc aggtcgaggc
      121 cggaaacgcc ttcctgctgc aggggggcga ctgcgccgag agcttcaagg agttctcgac
      181 cgacaacatc cgcgacacat tccgcctgat cctgcagatg gccgtggtcc tgaccttcgc
      241 cggcggcaag ccggtggtga aggtaggccg catcgccggc cagttcgcca agccgcgctc
      301 ctcctcgctg gaaaccatcg gcgacgagac cctgcccagc taccgcggcg acatcatcaa
      361 cggcatggcc ttcacgcctg aagagcggct gccggatccg cagcgcctgc tgcgcgccta
      421 caatcagtcg gcctccacgt tgaacctgct gcgcgccttc gccggaggcg gctatgccga
      481 cctgtacaac atccaccgct ggaccatggg gttcgcggcc gacagcgcct acggccacaa
      541 ataccgcgag ctggccgaca agatcaccga ggccctgacc ttcatggagg ccgtcggcgt
      601 cacgcccgaa agccatccgg acctcagccg ggtcgaggtc ttcaccagcc acgaagccct
      661 gctgctgaac ctcgaaagcg ccctgacccg cctcgacggc gcgacgggcg agtggttcga
      721 cacctcggcc cacatggtct ggatcggcga gcgcacccgc cagctggacg gcgcccatat
      781 cgagttcgcg cgcggcatca aaaatcccgt cggcctgaaa tgcgggccga ccatggagcc
      841 ggacgacctg ctgccgctga tcgacgccct gaaccccgac aatacgcccg gacgcctgac
      901 gctgatcggc cgcttcggcg cggacaaggt cggcgaccgc ctgccgcgtc tgatgaaggc
      961 ggtgaaggcc gagggccgcc gcgtggtctg gtcgatcgac cccatgcacg gcaacaccct
     1021 gaaggccggc aacggctaca agacccggcc gttcgaacgc atccttgcgg aggtccgggg
     1081 cttcatcgac gtggccgagg ccgaaggggt ccaccccggc ggcgtgcacc tcgagatgac
     1141 cggccagaac gtcaccgaat gcctcggcgg ggcccaggcc ctgaccgaag acgacctgtc
     1201 gagccgctac cacacccatt gcgacccccg cctgaacgcc gaccagtcgt tggagctagc
     1261 cttcctcgtc gccgaacggc tgaagtccgg acgccgatcg cagtcggagg cggcgtagaa
     1321 tggacggcgt cggcagacct gcaggcgacg acgtcggcgg ccgctgcgcc tatcagggcg
     1381 cgcccggcgc cttcagccac gaagcctgtc tggacctgcg cccctgggac gaggccgtcg
     1441 cctatgactc gttcgaagac gctatagcgg ctctgaagac gggcgaggtc ggttgcgccc
     1501 tgatcccggt cgagaacacg acgatcggtc gggtcgagcc ggcggcctcc ctcgtcgaag
     1561 cggccggcct gaccgtcgtt tccgaaatct ggcgtccgat tcgtctcgcc ttgatggccc
     1621 ctccgggcgc gaccctgcgc agcgtgaaaa cggcggaaag ccatcccgtc gccctgcggc
     1681 agtgccccga atccctggcg gccctgggcc tgacgccggt cgaggccttc gacaccgccg
     1741 gcgcggctcg cgccgtcgcc gaagccgctg acccgacccg cgccgccgtc gcccccacgc
     1801 gcgccgccga ggtctatgaa ctgtcgatcc tgcgcaacga tatccaggat tccgaggata
     1861 atcggacgcg atttgtcctg ctgagcgcca atccgggttg acgccgcacg gtccatgcgg
     1921 cctttgaggg ccatggtccg ctcgcaagcc accgctgcga ctgcattcgc ccgcgttttc
     1981 gcggccgcgg acctgtattt ctacggcttt cgatacgccg gctgaggcgt cgggaacatc
     2041 gtcctgacgg cctcgccggc gaccgccccg caacggggcg gcggctcgca aaacccctca
     2101 tcgccgtaat cgaaattgaa agccctttcc cgtgccccat tccagtacca agcccgccct
     2161 ccagcaagtc tggccttcgc cgaccggcgc gcccatgacc gacatctcgc ccgaacacat
     2221 ggccctggcg gccctgcgcc acgagatcga ccacctcgac gacgaactgc tggccctgtt
     2281 cgagcgccgt ctggccatcg ccgcccgcgt cggtcaggcc aaggacgccc ccgccggccc
     2341 tcatgtgaag ctgcgtcccg accgcgaaca ggcggtgctg gcgcgggtgc tggccaaggc
     2401 cggccccgac aaccgtgagg cggtggaatc cctgtggcgt cagatcgtcg gctgggggct
     2461 ggcgcgtcag ggccggatca aggtccaggt ctggtcgccc atcgagccga cccgcgcctt
     2521 cgacggcgcc cgcctgcgct tcggcgcggc cgccgacatg cgcgccgtcc gcgatccgga
     2581 ggccgccctg gcttgggctg cggaagggca tggcgtcgcc gtgctggcgg tcaacgccga
     2641 gagcgcttgg tggattggcc tgcgccgtga ctggtcgatg ctgagcgtgt tcgacggctt
     2701 cggcggcgcg acgccgacgg cgctggcggt cggcaagatc gatccggcgg cg
//
LOCUS       DASVVN010000428         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_175040,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000428 DASVVN010000000
VERSION     DASVVN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175040"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF686_06850"
     CDS             complement(<1..313)
                     /locus_tag="VF686_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661568.1"
                     /translation="MARASRAVSAVHAYPSPVRDPAGFRRAVLDLTARLDPVLIIPTC
                     EEVFHLAGLALGPVLFQPSAATLRSLHDKAAFAALCGSASLPAPETHAIDQAADLLPF
                     RD"
     gene            complement(420..1649)
                     /locus_tag="VF686_06855"
     CDS             complement(420..1649)
                     /locus_tag="VF686_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX8661569.1"
                     /translation="MNAAAIARSWLTTRLAMAQDAGGLAERRARLWRAFQPVMRRTPA
                     LAAHAEASLDRVPITEVAELRADYGRWNSRGLSHAELHAAAADAERGGPGEVRPGVVC
                     GYSTGSGGARGLFVADRAERADYIGQISARLLPVRTLLKPVRVALMLRANSRLYSDSA
                     GAGRAFLHLPLETSPDEAAVALERFAPTVLIAPGSRLTGLLDHGSRLPTLQRLFYGAD
                     PMSGAERAWIRDRFGIRADPIYQATEGFIGAACVEGRLHLNEHSLSIELEPVAGTQGY
                     RPIVTDLRRRTQPNVRVRTDDYLELDPAPCGCGYAGRAVRPIMGRVTDIWRYGNVTVT
                     PPRLASCMDAIIPLPVAWQAVARRDHVELRLGTGVRTATVADALRRRLDLPVPVRLSD
                     TPPEQDGPKRRWMVGHG"
     gene            complement(1646..2431)
                     /locus_tag="VF686_06860"
     CDS             complement(1646..2431)
                     /locus_tag="VF686_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8661570.1"
                     /translation="MTLQGGSLCAATFPAMVGVIRHPEAGTLLFDTGYDAAFMRATRQ
                     WPERLYRLATPVDLGGDGDRDSWLASQGVAASEITGVIVSHFHGDHVAGLPGFPGAPL
                     YCARAGLERVRSGNRLQRVFQGLLGDLVPPDVEARARYFEDAAAAALPAAFAPITEGR
                     DLLGDGSLLAVELPGHCPGHWGLALRTHEDRHVLLAADAAWSGGAIATCTPPPRLTTA
                     FLGDTGPYRQTLALLHQTSLRNPGLAILPSHCAASAAAFRQDP"
     gene            complement(2485..>3012)
                     /locus_tag="VF686_06865"
     CDS             complement(2485..>3012)
                     /locus_tag="VF686_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8661571.1"
                     /translation="AADSGRMATTVVRPRAIVGPDDRVILPRLAELARRRRIPLIRNG
                     RALIELTDVRDAAEAILCAEAEIASVHGRAVNVSGGHPLSVREVALALAASLGYAPEL
                     TRVPLSLAWVAAVAAELTASGGRRPEPILTRYTLATLACSQTFDMEETARLIGFHPRH
                     DGLAALLSAAASLKR"
BASE COUNT          468 a         1020 c         1118 g          406 t
ORIGIN      
        1 cgtcccggaa ggggagaagg tctgccgctt ggtcgatggc gtgcgtctcc ggggccggca
       61 gactcgccga tccgcagagg gcggcgaacg cggccttgtc atgcaggctg cgcagcgtgg
      121 cggcggacgg ctggaacagc accgggccaa gcgcgaggcc ggcgaggtgg aacacttcct
      181 cgcaggtcgg aatgatcagc acagggtcca gacgcgcggt gagatcgagg acggcccgcc
      241 ggaacccggc cgggtcccgg accggggagg gataggcatg cacggcgctg acggcgcgag
      301 aggcgcgcgc cattctggac cgaacacagt cggccatatg gacctcgaag cctagcctac
      361 ggaaatccct cgccatatcg acggcggcgg gtgatcgggc gccggtgatg aggacgcgcc
      421 tagccatggc cgaccatcca tcgccgcttc ggcccgtcct gttcgggcgg cgtgtcggac
      481 agccggaccg ggaccggcag gtcgagccgg cggcgcaggg cgtccgcgac cgtcgccgtc
      541 ctgacgcccg ttcccaaccg caactcgaca tggtcgcgcc gcgcgaccgc ctgccatgcg
      601 acggggagcg ggatgatggc gtccatgcag gacgcgaggc gcgggggcgt gaccgtgacg
      661 ttcccatagc gccagatatc cgtcacccgc cccatgatcg gccggactgc ccgaccggcg
      721 tagccgcacc cgcagggggc tggatcgagc tcgaggtagt cgtcggtccg gacacgaacg
      781 ttgggctgcg tgcgccgtct gaggtctgtc acgatcggac gatagccctg cgtgcccgcc
      841 acgggttcga gctcgatcga cagactgtgt tcgttcaggt gcagccgtcc ctcgacgcac
      901 gccgcgccga tgaacccttc ggtcgcctga tagatcggat ccgcgcggat gccgaaccgg
      961 tcacgaatcc acgcccgctc agcgccgctc atcgggtccg cgccgtagaa cagtcgctgg
     1021 agcgtgggca gccgggaacc gtggtccagc aaccccgtca gccgcgaacc gggtgcgatg
     1081 agaaccgtcg gcgcgaaacg ctccagcgca accgccgcct catccgggct ggtctcgaga
     1141 ggcagatgca gaaaggcgcg gccggcgcct gcgctgtcgc tgtagagccg gctgttggct
     1201 ctcagcatca gggcgacccg cacgggcttc aacagcgtcc tgaccggcag gaggcgtgcg
     1261 gatatctggc cgatgtagtc ggcgcgttcg gccctgtcgg cgacgaacag tccgcgcgcg
     1321 ccgccgctgc cggtggaata accgcacacc acccccgggc gaacctcccc cgggcctccg
     1381 cgctcggcgt cggcggcggc tgcgtgcagt tctgcgtggc tcagaccccg gctgttccag
     1441 cgtccgtagt cggcgcgaag ctcggcgacc tcggtgatcg ggacacggtc aagagaggcc
     1501 tccgcgtggg cggcaagcgc gggcgtccgg cgcatgacgg gctgaaacgc gcgccagagt
     1561 ctcgcccttc gttcggccag gcccccggcg tcctgggcca tagcgagacg agtggtcagc
     1621 caggagcggg cgatggcggc ggcgttcacg ggtcttgccg gaaggcggcg gcggaggcgg
     1681 cgcagtgcga cggcaggatg gcgaggccgg ggttccgcag gctcgtctga tgcagcagcg
     1741 ccagcgtctg gcggtacggc ccggtgtcgc cgaggaaggc cgtggtcagg cgaggcgggg
     1801 gcgtgcaggt cgcgatggct cctccggacc aggcggcgtc cgccgccaac agcacgtgcc
     1861 ggtcctcgtg ggtgcgcagg gcgaggcccc agtgtcccgg acagtggccc ggcagctcca
     1921 ccgccagcag ggagccgtcg cccagaaggt cgcgtccctc ggtgatgggt gcgaacgccg
     1981 cgggcagggc cgcggcggcc gcatcctcga aatagcgggc ccgcgcctcg acgtcgggtg
     2041 gaacgaggtc gccgagcaga ccctggaaga cgcgttgcaa gcgatttccc gacctgaccc
     2101 gctcgagtcc ggcgcgggcg cagtagagcg gcgcccccgg aaagcccggc aggcccgcga
     2161 catggtctcc atggaaatga gacacgatca cgccggtgat ctcgctggcg gcgacgccct
     2221 gcgaagccag ccagctgtcc cgatcgccgt cgccgccgag gtcgaccggg gttgcgagac
     2281 gatagagccg ctcgggccat tgacgcgtcg cccgcatgaa cgcggcgtcg tagccggtgt
     2341 cgaacagcag cgttccggcc tcgggatgcc ggatgacgcc gaccatggcc ggaaaggtcg
     2401 cggcgcagag ggacccgccc tgcagggtca tcgcctctgg atgccggcag ctgcctcgcg
     2461 cgatccaccg gaagccgacg cgcttcatcg cttcaggctc gcggccgcgg agaggagcgc
     2521 cgccagtcca tcgtgacggg ggtgaaagcc gatcaaccgg gcggtttcct ccatgtcaaa
     2581 ggtctgggaa caggcgaggg tggccagggt gtagcgggtc aatatcggtt cgggcctcct
     2641 tccgcctgac gccgtgagtt cggccgccac agccgccacc caggcaaggg acaggggcac
     2701 tcgcgtcagc tcgggcgcgt atccaaggct ggcggccaag gcgagggcga cctcgcgaac
     2761 actcagggga tggcctcccg agacgttgac cgcccgcccg tgcacggatg cgatttcggc
     2821 ctcggcgcag aggatggctt ctgcggcgtc gcgcacgtcg gtgagttcga tcaaggcgcg
     2881 cccgttgcgg atcagcggta tccgacggcg ccgtgcgagt tcggcgagac gcggcaggat
     2941 gacgcgatca tcgggcccga cgatcgcgcg cggccgcacc acggtcgtcg ccatcctgcc
     3001 ggagtcggcg gc
//
LOCUS       DASVVN010000429         5961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_148279,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000429 DASVVN010000000
VERSION     DASVVN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148279"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..457)
                     /locus_tag="VF686_06870"
     CDS             complement(224..457)
                     /locus_tag="VF686_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661572.1"
                     /translation="MTPADPPAEDRAARLLAVRLLEIVGGLDLTSADGRAGIGALLSE
                     IERLSPGAIHQQAAGMDLRRLGWDAQAASGSAQ"
     gene            1043..2230
                     /locus_tag="VF686_06875"
     CDS             1043..2230
                     /locus_tag="VF686_06875"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8661573.1"
                     /translation="MSSQPIDVLIVGAGAAGMMCAIEAGRRGRSVRILDHARAPGEKI
                     RISGGGRCNFTNLGTGPANFLGENPRFATSALKRYSQHDFVKRVDRAGIAWHEKTLGQ
                     LFCDDSAKQIIRMLTDDMRAAGVVLSLGTSVERVERAADGYEAALSDGSVVKARSLVL
                     ATGGKSIPKMGATGWAYDAARAFGLRVTETRPALVPLTFEAGLLEQLTPLAGVAVDAV
                     VRGGGTSFAEAMLFTHRGLSGPAILQISSYWREGEAITVAMAPGEDVWARLKAAKEEN
                     GKQMVHTALGHIVPRRLAESVCEREGVKGKLAEVGDKTLRRLDAAVNAWTVKPVGSEG
                     YRTAEVTLGGVATDQLDQQTMEAKSVPGLYVIGEAADVTGWLGGYNFQWAWSSGWAAG
                     QVC"
     gene            2349..3101
                     /gene="trmD"
                     /locus_tag="VF686_06880"
     CDS             2349..3101
                     /gene="trmD"
                     /locus_tag="VF686_06880"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8661574.1"
                     /translation="MPFTATVLTMFPEAFPGPLGVSLIGTAWRERGLWSLETVDIRAF
                     STDTRGFLDDTPAGGGPGAVLKADVVARAVDSLSGPQRPLLYMSARGRPLTQARVKEW
                     AKADGITVLCGRFEGVDQRVLDARGFEEVSVGDAVLAGGEAAAMVVIEACVRLIPGVL
                     GQAESLSSESFEDGLLEHPQYTRPRTFEGLEIPEVLLSGDHKKVAAWREEQRRETTRE
                     RRPDLWTAHLATTPAGPDPAKSQVKSKKARGE"
     gene            3103..3531
                     /gene="rplS"
                     /locus_tag="VF686_06885"
     CDS             3103..3531
                     /gene="rplS"
                     /locus_tag="VF686_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX8661575.1"
                     /translation="MNILQTIAAEEKARLTAVRAIPDFQPGDTLRVMVKIKEGERERI
                     QAFEGVCIARAGGGINENFTVRKISFGEGVERVFPLMSPNIDAIEVKRRGVVRRAKLY
                     YLRDRRGKSARIAEKQTVRPVKGVVVPETGKAETAADTEA"
     gene            complement(3799..4200)
                     /locus_tag="VF686_06890"
     CDS             complement(3799..4200)
                     /locus_tag="VF686_06890"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEX8661576.1"
                     /translation="MKKYAYGWITLGFFLISIGLHWAFGWFAFVDEAREHGQVAELGP
                     YLTEMGRDTFENWQSEFLQLLWQVVGLAYFLYVGSPSSKENDDRMEAKIDALLELVGK
                     ERGLQLIETIDESHLRHHGHAEVHQGQVRPE"
     gene            complement(4417..5514)
                     /locus_tag="VF686_06895"
     CDS             complement(4417..5514)
                     /locus_tag="VF686_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HEX8661577.1"
                     /translation="MNFRPLMAAAAVAALLAGCATTEVDYQGEGAGFVGEGVPVQARL
                     ETPSVGTGGGEDAADDPAIWASAAPVTVNGHASPGFVAGTDKKAGLYIYGFDGQVLQF
                     LPEGLLNNVDVTEGLDIDGGPRVVLGASDRTPGRTGISLYAFDPAGTGANGVRPWGYI
                     PTDVVEPYGFCFARRGAEVHAVLVGHEGELRQYVLTVDAAGRPAAREIRRAAIGTISE
                     GCAADEATDALYIAEENVGLWRYGLDPASGDRRTLVQPVAPDRLVADAEGLTILADGA
                     ARYLISSSQGDSTFPVWRIDGAAPVWVGRFKVMDGTVDAVTGTDGLAALGGAVGAFPE
                     GVVVVQDDVNDVGTQNFKYIDWRDVKAALGL"
     gene            5755..>5961
                     /locus_tag="VF686_06900"
     CDS             5755..>5961
                     /locus_tag="VF686_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661578.1"
                     /translation="MKTMLLLGAALAPLAFAMPALAQSGPNADPQGPVSSLEEVIVTG
                     EPAMRNRTDDVVPTLSYDLEYFQRF"
BASE COUNT          959 a         1867 c         2215 g          920 t
ORIGIN      
        1 cgccccgggc agcccatccg gcggcaggtc gtggcgcggc cccacaggac gaagctcgag
       61 ccgcgctcgc gggcgatgcg tttcaggtcg gcgcggatgc gcaactcgca caggtcgcac
      121 tcggtataga ccagccagcg gttggcgatg atcgcgccga ccgtggggcc ggcgaacttc
      181 cagtcctgga ccgtgtagcg gtccggattg cgtcgggtct gagttattgg gcgctaccgc
      241 tcgctgcctg agcgtcccac cccaaccgtc gcaggtccat ccccgcagcc tgctgatgga
      301 tggcgccggg ggagaggcgt tcgatctcgg acagcagggc gccgatgccg gcgcggccgt
      361 cggcgctggt caggtcgagg ccccccacga tctcaagcag gcggacggcg aggaggcggg
      421 cggccctgtc ttcggcgggg ggatctgcgg gggtcatggc ggggactctc tgactgactg
      481 actgactgtg aagaccgacc cggggagagg aagagcattc cggcgagagg ggcgcaacgg
      541 gaatgttgcc ggtttgttct tttttgtatg gaggggtgga gcgctgagcc ttgtcatccc
      601 ggacgccgcg cagcggtgat ccgggacgct gcgccgatgg ggcggcctcc cggaaatcgc
      661 gcgcgattgt ccgggaggcg ggcgggggtt gcgcgctggc ctgacctgtg tcccggatcg
      721 ctgctgacgc agcgtccggg cggatgggtt tgtggggatg gccgtcccgg atcgcggctt
      781 cgccacgtcc gggatgacaa ggggtcgggc tcctccggcg atgggcagaa cttttttgct
      841 gacgccggaa ttcctgcggg cgtggaggtt tgcggggcgc gaagggtgtt ttgcgcgcct
      901 gaggaagcgg cggcgccata ctgatccgga tgcgcgaccg aaacgcccac cgaccgacgc
      961 ccggccccgg gcccgaatag acggagtaga ctctgtagac gctgtagact gtgtttttcg
     1021 ccgcccgtgc taaggcgcgc ggatgagctc gcaacccatc gatgtcctga tcgtcggcgc
     1081 cggcgcggcc gggatgatgt gcgccatcga ggccgggcgg cgggggcgca gcgtgcgcat
     1141 cctcgaccat gcgcgggcgc cgggcgagaa gatccgcatc tcgggcgggg ggcggtgcaa
     1201 cttcaccaac ctggggaccg ggccggcgaa ctttctgggc gagaacccgc ggttcgcgac
     1261 ctcggccctg aagcggtaca gccagcacga cttcgtcaaa cgcgtggatc gggcggggat
     1321 cgcctggcac gagaagaccc tgggccagct gttctgcgac gacagcgcca agcagatcat
     1381 ccgcatgctg accgacgaca tgcgggcggc gggcgtggtg ctgtcgctgg ggacgtcggt
     1441 ggagcgggtc gaacgtgcgg ccgacggcta tgaggcggcg ctgtcggacg ggtcggtggt
     1501 gaaggcgcgg tcgctggtgc tggccaccgg tggcaagtcg atccccaaga tgggggcgac
     1561 gggctgggcc tatgacgccg cgcgggcgtt cgggctgagg gtcacggaga cgcggccggc
     1621 gctggttccg ctgacgttcg aggcggggct gctggagcag ctgacgccgc tggcgggggt
     1681 ggcggtggat gcggtggtca ggggcggggg gacgtcgttc gcggaagcca tgctgtttac
     1741 gcaccggggg ctgagcgggc cggccatcct gcagatcagc tcatactggc gcgagggcga
     1801 ggcgatcacc gtggccatgg cgccgggcga ggacgtctgg gcgcggctga aggcggccaa
     1861 ggaggagaac gggaagcaga tggtgcatac ggcgctgggc cacatcgtgc cgcgccggct
     1921 ggccgagagc gtctgcgaac gcgagggcgt caagggcaag ctggccgagg tcggggacaa
     1981 gaccctgcgc cggctggacg cggcggtgaa cgcctggacc gtcaagccgg tggggtcgga
     2041 gggctatcgg acggccgagg tgacgctggg tggcgtggcc accgaccagc tggaccagca
     2101 gacgatggag gcgaagtcgg tgccgggcct gtatgtcatc ggcgaggccg ccgacgtgac
     2161 gggctggctg ggcggctaca atttccagtg ggcctggtcg tcgggttggg cggcggggca
     2221 ggtttgctga gtggctagtg gctagtggct aatggctagt gattggtggt tcgggatcgc
     2281 ggttagggct agtaggtccg gcgtccgctt caactagcca ctagccacta gccaccagcc
     2341 actccttcat gcccttcacc gccaccgtcc tgaccatgtt ccccgaggcg tttccggggc
     2401 cgctcggcgt gtcgctgatc ggcacggcgt ggcgcgagcg cgggctctgg agccttgaaa
     2461 cggttgacat tcgggccttt tccacagata cgcgcggctt cctggacgac acccccgcgg
     2521 gtggcggccc gggagccgtg cttaaggcgg acgtggtggc ccgggcagtc gatagcctgt
     2581 caggaccgca acggccgctt ttgtacatga gcgcccgggg tcggcccctg acccaggcgc
     2641 gcgtcaagga atgggccaag gcggacggga tcaccgtcct gtgcggccgg ttcgaggggg
     2701 tggatcagcg cgtgctggac gcgcgcgggt tcgaggaggt ctccgtcgga gacgcggttc
     2761 tcgccggcgg cgaggcggcg gccatggtgg tgatcgaggc gtgcgtacga ctgatccccg
     2821 gagtgctggg ccaggcggaa agcctgagtt cggaaagctt cgaggacggg cttctggagc
     2881 atccgcagta cacgcgaccg cggacgttcg aggggctgga gatacccgag gttctgctgt
     2941 ccggagacca taaaaaggtc gccgcatggc gcgaagagca gcggcgagag acgacccggg
     3001 agcggcggcc agacctctgg acggcgcatc tggcgactac ccccgcgggg cctgaccccg
     3061 ccaaatcaca ggtaaagagc aaaaaagctc gaggagaatg acatgaacat cctgcagacc
     3121 atcgcagccg aagaaaaagc ccgcctgacc gcggtccgcg ccatccccga cttccagccc
     3181 ggcgacacgc tgcgcgtcat ggtcaagatc aaggaaggcg agcgcgaacg catccaggcc
     3241 ttcgagggcg tctgcatcgc ccgcgccggc ggcgggatca acgagaattt caccgtgcgc
     3301 aagatcagct tcggcgaggg cgtcgagcgc gtcttcccgc tgatgtcgcc gaacatcgac
     3361 gccatcgagg tcaagcgccg cggcgtcgtc cggcgcgcca agctctatta cctgcgcgat
     3421 cgccgcggaa agtcggcccg gatcgccgag aagcagaccg tgcgtccggt caagggcgtg
     3481 gtggtgccgg aaaccggcaa ggccgaaacg gccgccgaca ccgaagcata agccgcttta
     3541 cagcgaccag aatgacgaag gccccggcgg aaccgccggg gcctttttcg tcgcggtcac
     3601 gccgatacgg ctaaggacgg tccggagcag caggatcggg aacggtgtcc ggcgatcgcg
     3661 tcgggtgatt gtctgacgac ggctcgccat caggatcgcg ccacacctcc ccgatgacat
     3721 agaacccccg gctcgcgagc tgggctatca ggttggcgcg gtcctgcgcg ctctggaatc
     3781 ccggctttcc ggcgctgttc attcaggccg cacctgcccc tgatgcacct cggcgtggcc
     3841 gtggtggcgc agatggcttt catcgatggt ttcgatcagt tgcagaccgc gttccttgcc
     3901 gaccagctca agcaaggcgt cgatcttggc ctccatccgg tcgtcattct cctttgagga
     3961 gggcgaaccg acgtagagaa aataggccag cccgacgacc tgccagagca gttgcaggaa
     4021 ttcggattgc cagttctcga acgtgtcccg gcccatttcg gtcaggtagg ggcccagttc
     4081 cgcaacctgg ccatgctcgc gcgcttcatc gacgaatgcg aaccagccga aggcccagtg
     4141 caggccgatc gagatcagga agaagccaag ggtgatccag ccataagcgt atttcttcat
     4201 cacggtcctc cgtcgccaga caacgagcgc gggagcccgg ttccgcgcgt cagccctgcg
     4261 ggcctgtgtc gtaaaacggc agaggttcag ggcccgttcc tatgtcggga gaccgacata
     4321 tggcgcgtag cgaggagaca acgcctcgga tctggctatg gcgacgcgcg cacgtccacg
     4381 caccggcgag gccggcgctg aggaacgccg ggcgttttag aggccgagcg ccgccttcac
     4441 atcgcgccag tcgatgtact tgaaattctg cgtgccgacg tcgttgacgt catcctgaac
     4501 cacgacgacg ccctcgggga aggcgccgac agcgccgccc agggccgcga ggccgtcggt
     4561 gccggtgacg gcgtcgaccg tcccgtccat gaccttgaaa cggccgaccc agacgggggc
     4621 cgcgccgtcg atgcgccaga ccgggaaggt cgagtcgcct tggctggagg agatcagata
     4681 gcgggcggcg ccgtcggcga gaatggtcag gccttccgcg tcggccacga gccggtcggg
     4741 ggcgacaggc tgaacaaggg tgcggcggtc gccagaggcc gggtcgaggc catagcgcca
     4801 caggccgacg ttttcctcgg cgatataaag ggcgtcggtc gcctcatcgg cggcgcagcc
     4861 ttcggagatc gtcccaatgg cggcccggcg aatctcgcgc gcggcgggac ggcccgcggc
     4921 gtcgacggtc aggacgtact ggcgcagttc gccctcgtgc ccgaccagaa cggcgtggac
     4981 ctcggcgccc cggcgggcga agcagaagcc gtagggttca accacgtcgg tggggatgta
     5041 gccccaaggc ctgacgccgt tggcgccggt tccggcgggg tcgaaggcgt agagggaaat
     5101 gcccgtgcgg cccggcgtgc ggtcgctggc gccgagcacg acccgcgggc cgccgtcgat
     5161 gtccaggcct tcggtgacgt cgacattgtt cagcaggccc tcgggcagga actgcagcac
     5221 ctgtccgtcg aagccataga tatacagccc ggccttcttg tcggtgccgg cgacgaagcc
     5281 cggactggcg tggccgttca ccgtcaccgg agccgcggag gcccagatgg cgggatcgtc
     5341 ggccgcgtcc tcgccgccgc ccgtgccgac ggagggggtt tccagacggg cctggaccgg
     5401 gacgccttcg cccacgaagc ccgccccctc gccctggtag tcgacttcag tcgtggcgca
     5461 gccggcgagc agggcggcga ccgcggcggc ggccatcagg gggcggaagt tcatgattgc
     5521 gtcctttccg gtgtgcgccc ggcctgacca cgcgacccgc ggcgggtcaa caccgcaacg
     5581 gtgacggttc ggcgacgccg cgatgaaagg cggtcaggcg gcggatcaac ggtcatcggg
     5641 gcgtcagcga actgcaaagc cggcgtggtg gaactgtcgc gctgacgttg tcggtcggcc
     5701 ctagctcggg cccgaaacga cgccctcggg gggcgccgct cgcggggata tccgatgaag
     5761 accatgcttc tgctcggcgc agcgctcgcg ccgctcgcct tcgccatgcc ggccctggcc
     5821 cagtcggggc ccaacgccga tccgcaggga ccggtgtcgt cgctggagga ggtgatcgtc
     5881 accggcgagc cggcgatgcg caaccgcacc gacgacgtcg tgccgacgct gtcctacgac
     5941 ctggaatatt tccagcgttt c
//
LOCUS       DASVVN010000430         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_281566,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000430 DASVVN010000000
VERSION     DASVVN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281566"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VF686_06905"
     CDS             complement(<1..1008)
                     /locus_tag="VF686_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8661579.1"
                     /translation="MSLALFAAPAFAGDPVAASAAPAAPAAPSRERVRMNQRVFDHVW
                     NEVRREYYDPHLHGVDWNAARQTWRPVALAAPDDRTLYRSLREMLNLLDDDHAGAAPP
                     AVARRQDQLRERRAAVGLSLRADREDQDSYVVEQVREGSPAAEAGVGVGWRLIADGAS
                     GWTPDQDVVEGRPLVLSMVDAEGAARTTVLNPRVMEPRPAFVADRTRPNVLVLRIEGF
                     EQGLGRWMGEQLAGLTPDTDVVLDLRGNPGGRLMEADAVLSCFLPVNRAWATRTSRSG
                     RRIELRTAGGCGPLTAPVANDVAVLVDDQSRSAAELTPAALQEAGRAVVVGEPTAGAV
                     LI"
     gene            complement(1110..>1759)
                     /locus_tag="VF686_06910"
     CDS             complement(1110..>1759)
                     /locus_tag="VF686_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661580.1"
                     /translation="APPEVHTRFMMIGDGPGGLDKDGDGQITREEFAAPMNDHFARMD
                     KDGDGRLSAEERSAGHGTGDHVFIRGPAGDGGPGVHRFELRSAGEPGEHVGHDVRAMV
                     LSGRGEGGAHGEHEIVIRGPGGEGSPRIISLPRGAGGEHNVMVRRLGGPDGEHKMDAD
                     NDRRISEAEFTGPLREAFTRMDADHSSFIEEGEWGQGHDVRVFSHRLETRQDRED"
BASE COUNT          259 a          626 c          609 g          265 t
ORIGIN      
        1 aataagcacg gctcccgccg tcggctcgcc gaccacgacc gcgcgaccgg cctcctgaag
       61 ggcggcaggc gtcagttcgg cggcgctgcg gctctggtcg tcgaccagaa cggccacgtc
      121 gttggcgaca ggcgcggtca acggcccgca tccaccggcc gtcctcagtt cgatccgccg
      181 gccgctgcgc gaggtgcggg tcgcccaggc ccggttcacg ggcaggaaac agctcagcac
      241 cgcgtccgcc tccatcaggc ggccgccggg attgccccga agatccagaa ccacgtcggt
      301 gtcgggggtc aggccggcca gctgctcgcc catccagcgg cccagcccct gttcaaagcc
      361 ctcaatccgc aggaccagaa cattgggccg ggtgcgatcc gcgacgaagg ccgggcgcgg
      421 ttccatgacg cgggggttca ggacagtggt ccgcgcggcg ccctcggcgt ccaccatcga
      481 caggaccagg ggcctgcctt cgaccacgtc ctgatccggc gtccagcccg atgcgccgtc
      541 ggcgatcagc cgccagccaa cccccacgcc cgcctcggcc gcgggcgaac cctcgcggac
      601 ctgttccacc acatagctgt cctgatcttc ccgatcggcg cgcagggaca ggcccacggc
      661 ggcgcggcgc tcgcgcagct gatcctgacg gcgcgccacg gcgggcggcg cggcgccggc
      721 gtgatcgtcg tccagcaggt tcagcatttc gcgcagcgag cggtacaggg tgcggtcgtc
      781 cggagcggcc agcgccacag gccgccaggt ctggcgcgca gcgttccagt ccacgccatg
      841 caggtgcggg tcataatatt cgcgccggac ctcgttccag acgtggtcga agacccgctg
      901 gttcatccgg acgcgttcgc ggcttggcgc ggcgggggcc gcgggggccg ccgatgcggc
      961 cacgggatcg cccgcgaacg ccggagccgc gaagagcgca aggctcatcg cggctgacac
     1021 ggccagtcgt tcacacaggc gacggaagat catggcgcca cttaaggggc gccttcgatg
     1081 actttcaaat cacgaagtcg ctggacggat cagtcttcac ggtcctgtcg tgtttcgagc
     1141 cggtggctga agacccggac gtcgtgaccc tgaccccact cgccttcctc gatgaagctg
     1201 gaatgatcgg cgtccatgcg ggtgaaggct tcgcgcaggg ggccggtgaa ctcggcctcg
     1261 ctgatgcggc ggtcgttgtc ggcgtccatc ttgtgttcgc cgtcgggacc gccgagccgg
     1321 cgaaccatga cgttgtgctc gccaccggcg ccgcgtggca gagagatgat gcgggggctt
     1381 ccctcgccgc cgggaccgcg aatgacgatt tcatgctcgc cgtgagcgcc gccttcgccg
     1441 cggcctgaga ggaccatggc gcgaacgtca tgcccgacgt gctcgcccgg ctcgccagcg
     1501 ctgcgcagtt cgaagcggtg gacacccgga ccgccgtcgc cggcgggacc gcgtatgaac
     1561 acgtgatcgc ccgtgccatg gcctgccgag cgttcctcag ccgaaaggcg accgtcgccg
     1621 tccttgtcca tgcgggcgaa atggtcgttc atcggcgcgg cgaactcttc gcgcgtgatc
     1681 tggccgtcgc cgtccttgtc gaggccgccc gggccgtcgc cgatcatcat gaagcgggta
     1741 tggacctcgg gcggggccg
//
LOCUS       DASVVN010000431         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_148395,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000431 DASVVN010000000
VERSION     DASVVN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148395"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF686_06915"
     CDS             <1..229
                     /locus_tag="VF686_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8661581.1"
                     /translation="IPTMVGAFGLDFWESREMITGDVASLIGIGFVVSFISGLIVVRF
                     LVDFVGRYGFAPFSVWRIIVGTVGLLAIWLY"
     gene            complement(234..1202)
                     /locus_tag="VF686_06920"
     CDS             complement(234..1202)
                     /locus_tag="VF686_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX8661582.1"
                     /translation="MSEVAPGLVTVFGGSGFVGSQLVRALARRGWRIRVACRRPDRAW
                     KLQTSGSVGQIQAVRCDATNPDDVANALLGADAAVNLIGILYEAGRRSFHALHVEASR
                     NIAEAVAAAGIDRLVQVSAIGADPESESDYALSKAAAEMAVREVKPDAVVIRPSIVFG
                     AGDGFLNRFASMAQYSPFLPLIGGGQTKFQPVYVGDVAEAIARATVLPEAAGRTFELG
                     GPAVMTFEDVLKLILRETYRSNGLIPLPFFAARAIGSLAQLTAMVGIPPMLTRDQVVL
                     LEKDNVVADGAEGLAQLGIQPTGVEAIAPSYLWRYRRGGQFAEAAA"
     gene            1382..1468
                     /locus_tag="VF686_06925"
     tRNA            1382..1468
                     /locus_tag="VF686_06925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1416..1418,aa:Leu,seq:cag)
     gene            1628..2242
                     /locus_tag="VF686_06930"
     CDS             1628..2242
                     /locus_tag="VF686_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8661583.1"
                     /translation="MTVHFHEGDLPNGLDLGPEVAIDSETMGLRFRRDPLCVVQLSAG
                     DGDAHVVRMNRPGYDCPNLKRLLTDPAVLKLFHFGRFDIGMFQLHLGVETRPVYCTKI
                     ASRLARTYTDRHGLKDLVRELVGIDLSKAQQSSDWGAAELSPAQLEYAASDVLYLHAA
                     KVKLDEMLAREDRAELARKCFDFLPSRSALDLAGWDETDIFAHS"
     gene            2242..2898
                     /gene="lptC"
                     /locus_tag="VF686_06935"
     CDS             2242..2898
                     /gene="lptC"
                     /locus_tag="VF686_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX8661584.1"
                     /translation="MTDPADTRDQADEARVAATAKAFRARSRRVQLLRRILPVAIIVL
                     AGGTVSWIVLRSVMSDVERKAGTSREVRLDSAMFHGQDAEGRTFMIGAKSAIRDPETG
                     QFRLIGPALKLNLGGRKVTTLTADGGSYNEAAKSVTIGPNVRISDGGSGFVLVTPEAV
                     VNTATGVVTGSKGVQGSGPLGTISASSYAIYEQGQRIVFSGAGDNKIRGTINAGGSSR
                     "
     gene            2901..3377
                     /locus_tag="VF686_06940"
     CDS             2901..3377
                     /locus_tag="VF686_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX8661585.1"
                     /translation="MSRISSAAAVALALALIPAAGEAQSPGNSSDAPISFGADSGEYV
                     GNTVTLRGRAELIQGQNRLRADSVSGLSQTGESRIQANGNVYFVTPEQTIRGDSAVYT
                     TADDMIVVTGDVILTQGQNVLTGSRLTYNVRTQTARIEAGGQGRIRGVFYPQNSGN"
     gene            3429..>4076
                     /gene="lptB"
                     /locus_tag="VF686_06945"
     CDS             3429..>4076
                     /gene="lptB"
                     /locus_tag="VF686_06945"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX8661586.1"
                     /translation="MNEAPVEPAVSAPARATGLRVESIARSFGQRQVVRDVSLHVQRG
                     QVAGLLGPNGAGKTTCFYMITGLIPPDTGTIWLDGEDITAQPMYQRARMGLGYLAQEA
                     SIFRGMTVEQNVKAVVELHHTGATAIADETSRLLEELRIDHLRHAPASGLSGGERRRC
                     EIARALAGKPSFMLLDEPFAGIDPLAITDIREVIRYLKGRGIGILITDHNVRETLG"
BASE COUNT          686 a         1362 c         1403 g          625 t
ORIGIN      
        1 catcccgacc atggtcgggg ccttcggcct ggatttctgg gaaagccgcg agatgatcac
       61 cggcgacgtc gccagcctga tcgggatcgg cttcgtcgtg tccttcatca gcggcctgat
      121 cgtggtccgc ttcctcgtgg acttcgtcgg tcgctacggc ttcgcgccgt tctcggtctg
      181 gcgcatcatc gtggggacgg tcggccttct ggcgatctgg ctgtactagg ccgtcaggcg
      241 gcggcttcgg cgaactgacc gccacggcgg tagcgccaca ggtaggacgg ggcgatggcc
      301 tcgacgcccg tcggctgaat gcctagctgg gccagaccct cggcgccgtc ggcgacgaca
      361 ttgtcctttt ccagcaggac cacctgatcg cgggtcagca tgggcgggat gcccaccatg
      421 gccgtaagtt gcgcaagcga accgatggcg cgcgcggcga agaagggcag ggggatcagg
      481 ccgttcgagc ggtaggtctc gcgcaggatc agcttcagca cgtcctcgaa ggtcatgacg
      541 gccggaccgc cgagttcgaa ggtgcggccc gccgcctcgg gcaggaccgt ggcccgggcg
      601 atcgcctcgg ccacgtcgcc gacgtagacg ggctggaatt tcgtctggcc gccgccgatc
      661 agcggcagga agggcgagta ctgagccatg ctggcgaagc gattcaggaa gccgtccccg
      721 gcgccgaaga cgatcgaggg acgaatcacc accgcgtccg gcttgacctc gcgcaccgcc
      781 atctcggccg cggccttgga cagggcgtag tcggactcgc tctcgggatc ggcgccgatg
      841 gccgaaacct ggacaaggcg gtcgatgccg gcggcggcga cggcctccgc gatgttgcgc
      901 gacgcctcga cgtgcagggc atggaagctg cggcggccgg cttcgtacag gatgccgatc
      961 agattgacgg ctgcgtcggc gccgagcagc gcattggcca catcgtccgg attggtcgca
     1021 tcgcagcgta cggcctggat ctggccgacg gagcccgagg tctgcagctt ccacgcgcgg
     1081 tcggggcggc ggcaggccac gcgaatgcgc cacccccggc gggcgagggc gcgcaccagc
     1141 tggctgccga cgaaacccga accgccgaaa acggtgacca ggccgggggc gacttcgctc
     1201 atcacaggga ctccaacagg cggcggcggg cgcggggcag accctgcgcc ggggccggat
     1261 tagacgatgg ccgcgggcct cgcaacgcaa agccgataaa agcctgttga cccgcttggg
     1321 ggcctgcctt aagggtccgc gccttccgga cggaaacgca cgcaagtgcg aaaacgtctg
     1381 ggcccaggtg gcggaattgg tagacgcgct ggcttcaggt gccagtgata gaaatgtcgt
     1441 ggaggttcga gtcctctcct gggcaccact tttgaacggc gctgcatcga tgatgcggcg
     1501 ccgtttgcat ttagccctat cgcgccgggc gcggcccgac gctacttgag ggccggctat
     1561 cgcctcgcga gcagcgagag ggtagttgaa ggcgtaaccg cgaccgcagc ctgagacctg
     1621 accccgaatg accgtccatt tccacgaagg cgacctgccg aacggcctgg atctggggcc
     1681 cgaggtggcg atcgattccg agaccatggg cctgcgtttc cgccgtgatc ccctgtgcgt
     1741 ggtccagctg tcggccggcg acggcgacgc ccatgtcgtg cgcatgaacc gccccggcta
     1801 cgactgcccg aacctgaagc ggctgctgac cgatccggcg gtgctgaagc tgttccactt
     1861 cggccgcttc gatatcggca tgttccagct gcacctcggc gtcgagaccc ggccggtcta
     1921 ctgcaccaag atcgcctcgc ggctggcccg cacctacacc gaccgccacg gcctgaagga
     1981 cctggtccgc gaactggtcg ggatcgatct gtccaaggcc cagcagtcca gcgactgggg
     2041 ggcggccgag ctgtcgccgg cccagctgga atacgccgcg tccgacgtcc tgtacctgca
     2101 cgccgccaag gtgaagctgg acgagatgct ggcgcgcgag gatcgcgccg aactggcccg
     2161 caaatgtttc gacttcctgc ccagccggtc tgcgctggat ctggcgggat gggacgagac
     2221 ggacatcttc gcgcacagct gatgacagac cccgccgaca cccgggatca ggccgacgag
     2281 gcgcgcgtcg ccgcgacggc caaggcgttc cgcgcccggt cgcgacgggt ccagctgctg
     2341 cgccgcatcc tgccggtggc gatcatcgtc ctggccgggg gcacggtcag ctggatcgtg
     2401 ctgcgcagcg tgatgtcgga cgtcgagcgc aaggccggga ccagccgcga ggtccggctg
     2461 gacagcgcca tgttccacgg tcaggacgcc gagggccgca ccttcatgat cggcgccaag
     2521 agcgccatcc gcgatcccga gaccggccaa tttcgcctga tcggaccggc gttgaagctg
     2581 aacctcggcg gccgcaaggt cacgaccctg acggccgacg gcggctccta caatgaggcc
     2641 gcgaaatcgg tcaccatcgg ccccaatgtg cggatatccg acggcggttc gggttttgtc
     2701 ctcgtcacgc ccgaggcggt ggtgaacact gcgaccggcg tggtgacggg atcgaagggc
     2761 gttcaggggt ccggccccct tggaaccatc tccgcttcgt cctatgcgat ttatgaacag
     2821 ggtcagcgga tcgtgttcag cggcgcgggt gataacaaga tacgcggcac catcaacgcc
     2881 ggagggtcca gcagatgagc atgagcagaa tttcgagcgc ggcggccgtt gcgctggcct
     2941 tggccctgat cccggcggcg ggcgaggccc agagccccgg gaacagcagc gacgccccga
     3001 tctcgttcgg ggcggactcc ggcgaatacg tcggcaacac cgtcaccctg cgcggacggg
     3061 ccgagctgat ccagggtcag aaccgcctgc gcgccgacag cgtctcgggt ctgagccaga
     3121 cgggcgaaag ccgcatccag gcgaacggca acgtctattt cgtcaccccc gagcagacga
     3181 tccgcggcga cagcgccgtc tatacgaccg ccgacgacat gatcgtggtg acgggggacg
     3241 tcattctgac ccaaggccag aacgtgctga ccggctcgcg cctgacctac aacgtccgca
     3301 cccagacggc gcggatcgag gcgggcggac agggacgtat cagaggggtc ttctatcccc
     3361 agaacagcgg gaactgagcc tcggctcgga cctgacattt ggcgcgcaag gaactctcag
     3421 ccctcaatgt gaacgaagcc ccggtcgagc cggcggtttc ggcgcccgcg cgcgccacag
     3481 gtctgagggt cgagagcatc gcccgatcct tcggccagcg gcaggtggtg cgcgacgtct
     3541 ccctgcacgt tcagcgcggt caggtggccg gcctgctggg tccgaacggc gctggcaaga
     3601 cgacctgttt ctacatgatc accggcctga tcccgccgga caccggcacg atctggctgg
     3661 acggcgagga catcacggct caaccgatgt accagcgcgc ccgcatgggt ctgggctatc
     3721 tggcgcagga ggcctcgatc ttccgcggca tgacggtcga gcagaacgtc aaggcggtgg
     3781 tcgagcttca tcacacgggc gcgaccgcca tcgccgacga gaccagccgc ctgctggaag
     3841 agctgcgcat cgaccatctg cggcacgccc cggcctcggg cctgtcgggc ggcgagcggc
     3901 gccgctgcga gatcgcccgc gccctggccg gcaagccgtc cttcatgctg ctggacgaac
     3961 ccttcgcggg catcgacccc ctggccatca cggacatccg cgaggtgatc cgttacctga
     4021 agggacgcgg catcggcatc ctgatcaccg atcacaacgt gcgcgaaacc ctgggc
//
LOCUS       DASVVN010000432         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_175239,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000432 DASVVN010000000
VERSION     DASVVN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175239"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF686_06950"
     CDS             complement(<1..309)
                     /locus_tag="VF686_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661587.1"
                     /translation="MKRLLTAALCAVLGAGAATAAPQDDDWEFQEDPAQHLMVAAVRY
                     EAGAAIIVQCRAGALTAVLVGLTPSDSGLELRAARSDGRRDTQVWATAGAPGAFRSET
                     A"
     gene            475..1188
                     /locus_tag="VF686_06955"
     CDS             475..1188
                     /locus_tag="VF686_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX8661588.1"
                     /translation="MPDRLQLNRRAVLATGAAGLMLPGCASVRPQTAAGQKVWTFDTL
                     TSVGGAATAAEGGPTLVDSPFGPAVRFDGVDDALFIEEHPLAGAATWTIEALIRPDGG
                     AFEQRFLHLAETETPREGQPLVGTRFLFEIRVVENEWYLDAFTKGPTYNHTLIFPGKR
                     FPVGRWRHVAQTYDGTTYRGFVDGVLQGEAPLAFTPQGRGRSSVGVRMNRVSWFNGAI
                     REARFTPRGLTPDQFTRPT"
     gene            complement(1172..1711)
                     /locus_tag="VF686_06960"
     CDS             complement(1172..1711)
                     /locus_tag="VF686_06960"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8661589.1"
                     /translation="MRNVQPETLTLDRVATPTGEVLLITDAEGAVRALDFAGYEERMM
                     RLLRRHWPKASLVEGRAPTAVRSAVEAYFSGDAAALDGLQVRTNGTDFQRSVWAALRA
                     IPHGETRTYGQLAAAIGKPKAVRAAGLANGQNPIAVIVPCHRVIGANGTLTGYAGGVE
                     RKQWLLAHERQCAGVRSAA"
     gene            complement(1692..2807)
                     /locus_tag="VF686_06965"
     CDS             complement(1692..2807)
                     /locus_tag="VF686_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX8661590.1"
                     /translation="MENASELDQEACYRAVLTRDARFDGRFFGCVKTTGIYCRPVCPA
                     RTPKRENMFFVVTAAAAEEAGFRACLRCRPETAPDMGAWRGTSNTVSRALALIEAGAL
                     DEGDVDRLAARLGVGERHLRRLFRQHLGAAPVSVAQTRRVLLAKALIHQTDLSMAQVA
                     LASGFGSVRRFNETFQALYERPPSALRRRGERVHEGGVKLSLSYRPPYDWEAMFAALS
                     ERGDTVEGRRWSRVLTPDVDGAEGSVTVEPAEAGKVAICIEAADLKVLPGVLARVRRV
                     FDLSADPDAIARDLSADPVLKPLVEARPGLRLPGLWIDDDLEAPSDRLPDAWIDETLS
                     ARAEAWRPWRAYGVLYLSLAGISDAELMERDDAKRAA"
BASE COUNT          427 a         1070 c         1005 g          426 t
ORIGIN      
        1 cgccgtctcg ctgcggaagg ctccgggcgc gcctgccgtc gcccagacct gggtgtcgcg
       61 ccgcccgtcg gagcgcgctg cgcgcaattc cagcccgctg tccgaggggg tcaggccgac
      121 cagcaccgcg gtgagcgccc cggcgcggca ttgcacgatg atcgccgccc cggcttcata
      181 ccggactgcg gcgaccatca ggtgctgcgc ggggtcctcc tggaactccc agtcgtcgtc
      241 ctgcggcgca gccgtcgccg cgcccgcgcc cagcacggcg caaagcgccg cagtcagaag
      301 ccgcttcatt gtcatattcc ccgttcccgc cgccagtgga gacggcgcag cggattcgtc
      361 aagaccgtca ggcgagggtg gtcgtggtcc agaccgtcgt caggtcggcc ggctgcaaat
      421 tccgggcggc gcggcggctc ccggtctaca caaggcccat aagggagaag ccggatgcct
      481 gacagacttc agttgaaccg ccgggctgtg ctggccacgg gcgccgccgg cctgatgctc
      541 cccggctgcg cgtcggtccg cccgcaaacg gcagccgggc agaaggtctg gacgttcgac
      601 actctcacga gtgtcggtgg cgcggcgacc gctgccgagg gcgggccgac gctggtcgac
      661 agtccctttg gcccggcggt gaggttcgac ggcgtggacg acgccctgtt catcgaggag
      721 cacccgctgg cgggcgcagc gacctggacg atcgaagccc tgatccggcc ggatggcggc
      781 gctttcgagc agcgcttcct gcacttggcc gagaccgaga cgccgcggga aggtcagccg
      841 ctcgtcggga cccggttcct gttcgagatc cgggtcgtcg agaacgaatg gtacctcgac
      901 gccttcacca aggggccgac ctacaaccac accctgatct tccctgggaa gcggttcccg
      961 gtcgggcgct ggcgccacgt cgcccagacc tatgacggaa cgacctatcg cggcttcgtc
     1021 gacggcgtcc tccagggcga ggccccgctg gccttcacgc cgcaggggcg ggggcggtcg
     1081 tcggtcgggg tgcggatgaa ccgggtcagc tggttcaacg gcgcgatccg cgaggcgcgc
     1141 ttcacgcccc gcggtctgac gccggatcag ttcacgcggc cgacctgacg cctgcgcact
     1201 ggcgctcatg cgccagcagc cactgtttgc gctccactcc cccggcgtag ccggtcagcg
     1261 tcccgttcgc gccgatcacc ctgtgacagg gaacgatcac tgcgatcggg ttctgcccat
     1321 tcgccagacc cgccgcccgc accgccttcg gcttgccgat cgccgccgcc aactgaccgt
     1381 aggtccgcgt ctccccgtgc ggaatggcgc gcagggccgc ccagaccgaa cgctggaaat
     1441 cggtgccgtt ggtgcggact tgcaacccgt ccagcgcggc ggcgtcgccg ctgaaatatg
     1501 cctcgaccgc cgaccgcacc gcggtggggg cccgcccctc gaccagcgac gctttcggcc
     1561 agtggcggcg caacagccgc atcatccgct cctcgtagcc ggcgaagtcc agcgcccgca
     1621 cggcgccttc cgcatcggtg atcaacagca cctcgccggt cggcgtggcg acccggtcca
     1681 gggtcagggt ttcaggctgc acgtttcgca tcgtctctct ccatcagttc ggcgtcgctg
     1741 atgcccgcga gggacagata cagcacgcca taggcccgcc acggacgcca ggcttcggcg
     1801 cgagcggaca gggtttcgtc aatccaggcg tctggcagcc gatcgctcgg cgcctccaga
     1861 tcgtcgtcga tccacagccc cggcaggcgc aggcccggcc gggcctcgac cagcggtttc
     1921 agcaccgggt cggcggacag gtcgcgggcg atggcgtccg gatcggccga caggtcgaac
     1981 acccgccgga cccgcgccag cacccccggc agaaccttca gatcggcggc ctcgatacag
     2041 atcgcgacct tgcccgcctc cgccggttcg accgtgaccg agccctcggc tccgtccacg
     2101 tcgggggtca ggacacgcga ccagcgccgc ccctcgaccg tgtcgccccg ctcgctcagg
     2161 gcggcgaaca tcgcctccca gtcatagggc ggacgatagg acaggctcag cttcaccccg
     2221 ccctcatgaa cccgctcgcc gcgccggcgc agggccgagg gcgggcgttc gtacagggcc
     2281 tggaaggtct cgttgaagcg ccgcacgctg ccgaagcccg aggccagggc cacctgcgcc
     2341 atgctcagat cggtctggtg gatcagcgcc ttggccagca gcacccggcg ggtctgggcc
     2401 acgctgaccg gcgccgcgcc cagatgctgg cggaacagcc ggcgcaggtg tcgctcgccg
     2461 acgcccagcc gcgcggccag acggtcgacg tcgccttcgt cgagcgcgcc cgcctcgatc
     2521 agggccaggg cccggctgac cgtattggag gtgccgcgcc aggcgcccat gtccggcgcc
     2581 gtctcggggc ggcagcgaag gcaggcgcga aaccccgcct cctcggccgc cgccgccgtg
     2641 acgacgaaga acatgttctc ccgcttcggg gtccgggcgg ggcagaccgg gcggcagtag
     2701 atgccggtgg tcttcacgca gccgaagaag cgcccgtcga accgcgcgtc gcgggtcagc
     2761 accgcgcggt aacaggcctc ctggtcgagt tcgctggcgt tttccatggc cgcaagatgg
     2821 gcccggcggc gccggatgtc tcgcggtttt cggacatgac gcccagcaat gctgggttgt
     2881 aaataccgcc ggttggtgaa actgtagcgt cgaatgggga ggcgaaga
//
LOCUS       DASVVN010000433         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_121798,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000433 DASVVN010000000
VERSION     DASVVN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121798"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VF686_06970"
     CDS             <1..1092
                     /locus_tag="VF686_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661591.1"
                     /translation="PGTRGAPRVGDTVVDCNLTPIEEFARKKLDLWEGNLETEAGRVT
                     SAPYLMWNRNNPFAAGVPSICSFQSGRGRPREIQIRPIPVVAGDLEAAYRASVYMPPA
                     TPLGVETVNGRPWVHVHSFADSADWRSFNAAVEGQAATLRGPQGFVLDLRAASGTGQN
                     SSTARGYGLANRIWTPEFTVSRQPAAGELTYRATQGNRDWYAAALGRMEADPQFVQEA
                     GPVIEQTREIVAAFDAAIAAGQPTFTLAGRAAVADTGAANPVQGPVIVLVDAGCSGGC
                     LDTLDLLSKLPNVRIAGSTTATDSIFVEPTVMRLPSNYSDLSYGHKAWTSRPRPNNQP
                     FTPAQGLVYTGDPADETAVRTWVGGLFGG"
     gene            complement(1168..1344)
                     /locus_tag="VF686_06975"
     CDS             complement(1168..1344)
                     /locus_tag="VF686_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661592.1"
                     /translation="MTKKPEPDLITDPLTPRATHGRVEIKDPPESAMNLTADAAEISG
                     LRLVDAADRARKRD"
     gene            1422..1913
                     /gene="msrB"
                     /locus_tag="VF686_06980"
     CDS             1422..1913
                     /gene="msrB"
                     /locus_tag="VF686_06980"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8661593.1"
                     /translation="MTDNDIAPILRSPSGYDLTPPSESQRVALEADLSAEEKRVLLAH
                     GTEAPFCGTLLGEKRAGVFCCRECGLPLFRTGTKFESGTGWPSFTEPVDPTHVTAISD
                     HSYGMTRTETRCARCDSHQGHVFPDGPGPTGLRYCINSVALSFVPEGSPLPDPLGRGD
                     GNA"
     gene            1963..>2228
                     /locus_tag="VF686_06985"
     CDS             1963..>2228
                     /locus_tag="VF686_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661594.1"
                     /translation="MLITALLMAGALTAGDPDGVVATAPATSVDLGATGTPDAPSVVG
                     AVQAAVPHNLTTAEQVDRWIGARSVESKPYDRPWGESPDDGLPHG"
BASE COUNT          334 a          796 c          756 g          342 t
ORIGIN      
        1 cccggcaccc gcggcgcgcc gcgggtcggc gacacggtgg tcgactgcaa tctgaccccg
       61 atcgaggaat tcgcccgcaa gaagctggac ctgtgggaag gcaacctcga gaccgaggcg
      121 ggccgcgtga cctccgcgcc ctatctgatg tggaaccgca acaacccgtt cgccgccggc
      181 gtgccgtcga tctgcagctt ccagtccggc cgcggccgtc cgcgtgaaat ccagatccgt
      241 ccgatcccgg tggtcgccgg agacctcgag gccgcctacc gcgcctcggt ctatatgccg
      301 ccggcgacgc cgctgggcgt cgagaccgtc aatggtcgtc cgtgggtgca tgtccactcc
      361 ttcgccgaca gcgccgactg gcgctcgttc aacgccgccg tcgagggtca ggccgccacc
      421 ctgcgcgggc cgcaaggctt cgtgctggat ctgcgcgccg cctcgggcac cggccagaac
      481 tcatcgaccg cgcgcggcta tggtctcgcc aaccgcatct ggacgccgga gttcaccgtc
      541 agccgccagc cggccgccgg ggaactgacc taccgcgcca cccagggcaa ccgcgactgg
      601 tacgccgccg ccctgggccg aatggaggct gacccgcagt tcgtgcagga agccggtccc
      661 gtgatcgagc agacgcgcga gatcgtggcg gccttcgacg ccgctatcgc cgccggccag
      721 ccgaccttca ccctggccgg acgcgccgcc gtggccgaca cgggcgccgc caaccccgtg
      781 cagggtccgg tgatcgttct ggtggacgcc ggctgctcgg gcggctgcct cgatacgctg
      841 gacctgctgt ccaagctgcc gaacgtgcgc atcgccggct cgaccacggc gaccgacagc
      901 atcttcgtcg aaccgaccgt gatgcggctg ccttcgaact attcggacct cagctatggt
      961 cacaaggcct ggacctcgcg tccgcgcccc aacaaccagc ccttcacccc cgcgcaaggc
     1021 ctggtctaca cgggcgaccc cgccgacgag acggcggtgc ggacctgggt cggcggcctg
     1081 ttcggcggct gatccggacc tcgtggtcac atgaagaggc cggtcagggc agcctgaccg
     1141 gccttttttc atgtccggcc cgggtggtca gtcgcgtttg cgggcgcggt cggcggcgtc
     1201 gacgaggcgc aggccggaaa tctccgcggc gtccgccgtc aggttcatgg ccgactcggg
     1261 cggatccttg atttccacgc gcccgtgggt cgccctgggc gtcagcggat cggtgatcag
     1321 gtcgggctcg ggtttcttcg tcatgcgtta cgaacgattg ccgacataag ccgttccggc
     1381 ggcgggattg cgaaaagacc cggagcgcct aggtgacacc catgaccgac aatgacatcg
     1441 ccccgatcct gcgttccccg tccggctacg acctgacgcc tccgagcgag agccagcggg
     1501 tggcgctgga ggccgacctg tcggccgagg agaagcgggt cctcctggcg cacggcaccg
     1561 aggcgccctt ctgcgggacc ctgctgggcg agaagcgggc gggggtgttc tgctgccgcg
     1621 aatgcggcct gccgctgttc cgcaccggga ccaagttcga aagcggcacg gggtggccca
     1681 gcttcaccga gccggtggat ccgacgcatg tgaccgcgat cagcgaccac tcctacggca
     1741 tgacgcggac ggagacccgc tgcgcccgct gcgacagcca tcagggccat gtgtttccgg
     1801 acggtcccgg gccgacgggt ctgcgctact gcatcaattc cgtggcgctg agcttcgtgc
     1861 ccgaaggttc gcccctgccg gacccgctgg gccggggaga cggaaacgcc tgaccttggc
     1921 ctcatttgaa gctagggtgg tagccgtaac ggagtagggc gcatgctgat caccgcactg
     1981 ttgatggccg gagccctgac ggccggcgat ccggacggcg tcgtcgcgac cgctcccgca
     2041 acctcggtcg atctcggtgc gacagggacg ccagatgcgc catcggtcgt cggcgcagtg
     2101 caggcggccg tccctcacaa cctcaccacc gccgaacagg tcgaccgctg gatcggcgcg
     2161 cgctctgtgg agagcaagcc ctatgaccgg ccttggggcg aatcgccgga tgacggcctg
     2221 ccccacgg
//
LOCUS       DASVVN010000434         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_121841,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000434 DASVVN010000000
VERSION     DASVVN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121841"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..543
                     /locus_tag="VF686_06990"
     CDS             181..543
                     /locus_tag="VF686_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661595.1"
                     /translation="MIRSLLAAGAAIVALALAPQPAAAASNPLEPAFKGTIISTYPDG
                     RTAKLWLNADGTYRGQGRRGKPSSGRWSLKGSKVCLKQSKPMGAPFAYCTPVPKGTTW
                     SAKAVTGEPIRVRLVPGR"
     gene            636..>1989
                     /locus_tag="VF686_06995"
     CDS             636..>1989
                     /locus_tag="VF686_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661596.1"
                     /translation="MRLGLLGGVSLAVAFGIASAASVATADSAADDVAAADDQATDVE
                     GVVVTGRRDSYRVEETPSATRTRTELKEVPQSVSVITEEVIDDQAMQGMADVLRYVPG
                     ATMGQGEGHRDAPTLRGNASTADFFVDGVRDDVQYFRDLYNVARVEVLKGPNAMIFGR
                     GGGGGVINRVTKRAEAGRSAELRVEAGSDEHMRITGDANAPLASGIFGRVNALYQSSD
                     SFRDYVEVERWGANPTVTFQNERAVLRLNYEHFEDDRIVDRGVPSFGGRPVTPSRSTF
                     FGDPGQSYAATDVDFFSGTVEYALSPVMTVRNHTVVADYFKFYQNVLPGPVSASGAQV
                     ALEAYKNRTDRQNLFNQTDVVLQANTGALRHTLLFGVELGRQETANFRNTGFFNNTST
                     SFLVPFANPTVFGAPITFRQSASDANNSTEANVAALYVQDQVWITPQLQLIAGLRFDR
                     F"
BASE COUNT          327 a          698 c          653 g          311 t
ORIGIN      
        1 gcctcacgga gaccgccatg cgcgtcatca agctcgtcct ggtcgtgctc gccgccgcgc
       61 tgccgatcgt cttcatggcc gcgacggaca gctggccgat ctgagccggc catcgcgtgc
      121 agcctgggca ccgggcgaac cgtggcgcgt ttgaccccgg gacgccaact cgggatgccc
      181 atgatccgta gtcttctcgc cgccggggcc gccatcgtcg ccctggcgct cgcgcctcaa
      241 ccggccgccg ccgccagcaa cccgctcgag cccgccttca agggcacgat catctccacc
      301 tacccggacg gccgcaccgc caagctctgg ctgaacgccg acggcaccta tcggggtcag
      361 ggccgccgcg gcaaaccctc cagcgggcga tggtccctga agggctcgaa ggtgtgcctg
      421 aagcagtcga agccgatggg cgcccccttc gcctactgca cgccggtgcc caagggcacg
      481 acctggtccg ccaaggcggt gaccggggag cccatccggg tccggctggt ccccggtcgc
      541 taggacgaac cgaacggcgc caagttgcga ttgacttgca aaagcagccc gagctaggga
      601 ctgcttttga gaattattcg caaaaagggt ttcacgtgcg gctcgggttg cttggcggcg
      661 tttcgctggc ggttgcgttt gggatcgcct cggcggcgtc ggtcgcgacg gcggacagcg
      721 ccgcagacga tgtggcggcg gccgacgacc aagccaccga tgtcgagggc gtcgtcgtca
      781 ccggccggcg cgacagctat cgcgtcgagg agacgccctc ggccacccgc acccgaaccg
      841 agctcaagga agttccccag agcgtcagcg tgatcaccga ggaggtgatc gacgaccagg
      901 ccatgcaggg catggccgac gtgctgcgct atgtgcccgg cgccaccatg gggcaggggg
      961 agggccatcg cgacgcgccg accctgcgcg gcaacgccag cacagcagac ttcttcgtcg
     1021 acggcgtgcg cgacgacgtc cagtacttcc gcgacctgta caacgtcgcc cgcgtcgagg
     1081 tcctcaaagg tccgaacgcg atgatcttcg gtcggggcgg tggcgggggc gtgatcaacc
     1141 gcgtgaccaa gcgcgccgag gccggccgct cggccgagct gcgcgtcgag gccggttccg
     1201 acgagcatat gcgcatcacc ggcgacgcca acgcgccgct ggcctccggg atcttcgggc
     1261 gcgtcaacgc cctctatcag agctccgaca gcttccgtga ctacgtggag gtggagcgct
     1321 ggggcgccaa tccgaccgtg accttccaga acgagcgtgc ggttctgcga ctgaactatg
     1381 agcatttcga ggacgaccgc attgtcgacc gcggcgtgcc ctcgttcggc gggcgccccg
     1441 tcactccctc gcgctcgacc ttcttcggcg atcccggcca gagctacgcc gccactgacg
     1501 tggacttctt cagcggcacg gttgagtacg cgctgtcgcc ggtgatgacg gtgcgcaacc
     1561 acacggtcgt cgccgactat ttcaagttct accagaacgt cctgccggga ccggtctcgg
     1621 cgtcaggcgc gcaggtcgcg ctcgaggcgt acaagaaccg cacggaccgt cagaacctgt
     1681 tcaaccagac cgatgtcgtg ctgcaggcga acacgggcgc gctgcgccac acgctgctgt
     1741 tcggcgtcga gctgggacgc caggagacgg cgaacttccg caacaccggc ttcttcaaca
     1801 acacgtcgac atccttcctc gtgccgttcg ccaacccgac cgtgttcggg gcgccgatca
     1861 ccttccggca gtcggcgagc gacgccaaca acagcaccga ggccaacgtc gcggcgctgt
     1921 acgtgcagga ccaggtctgg atcacgcccc agctgcagct gatcgcgggg ttgcgcttcg
     1981 atcgtttcg
//
LOCUS       DASVVN010000435         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_362189,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000435 DASVVN010000000
VERSION     DASVVN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362189"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /gene="lysA"
                     /locus_tag="VF686_07000"
     CDS             complement(<1..1107)
                     /gene="lysA"
                     /locus_tag="VF686_07000"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX8661597.1"
                     /translation="MHHFDRIDGALHAEGVPLEALAEAVGTPAYVYSTATLTRHYSLL
                     REAVDAHRDALGDALIAFAVKANSNLSVLATLARLGCGADTVSEGEIRRALAAGIPGD
                     RIIFSGVGKTDAELAFAIEAGVRQINVESGAELDRLIEVAASKGKAPAIAIRVNPKIG
                     AGGHAKITTGGATDKFGVPVEEAIDLYARASASPHLTPVGLACHIGSQITTLEPMEAA
                     FRTLRDVVLTLRANGHSVGRLDLGGGLGVPYHGQTDTPSIADYAAMCARVLDGLNVEA
                     AFEPGRLLAANAGLLLSRVIQVNQRGDGRRFLVLDAGMNDLMRPALYDAFHDLVPVRP
                     REGELLAYDLVGPICESTDIFARDRLLPPLEAGDL"
     gene            complement(1111..1347)
                     /locus_tag="VF686_07005"
     CDS             complement(1111..1347)
                     /locus_tag="VF686_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661598.1"
                     /translation="MKSIVSLGVLALFAVSAAGCGRMADLEAPPAKKTERSQRDARAP
                     GLPEPATVYRPPSTQPIDGGPSSPYNGPGAQPNR"
     gene            complement(1357..2724)
                     /gene="argH"
                     /locus_tag="VF686_07010"
     CDS             complement(1357..2724)
                     /gene="argH"
                     /locus_tag="VF686_07010"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX8661599.1"
                     /translation="MWGGRFSARPADIMQAINVSIGVDRRLWAQDLAGSRAHSRMLAA
                     RGIVTRADADAILGGLDAIEGEIRDGSFPFRDQFEDIHMNVEARLAELIGEPSGRLHT
                     ARSRNDQVATDFRLWVRDACDRAIEQLKGLQAVLLARAEQHAGDLMPGFTHLQTAQPV
                     TLGHHLMAYVEMFGRDAGRFADARQRMNENPLGAAALAGSPFPIDRHMTASELGFDRP
                     MANSLDAVSDRDFALESLAAASIAAGHLSRLAEEIVIWMTPMFGFASLPDDLTTGSSI
                     MPQKRNPDAAELVRAKTGRIMGSLVALSTVMKGLPLAYSKDMQEDKPPVFEAFDALTL
                     ALGAMTAMVAALRPDTERMRAAAGAGFSTATDLADWLVRELNLPFRKAHHVTGAAVKR
                     AEALGVDLSQLPLSELQSLEAGVTEAVYKVLTPEASCASRQSFGGTAPEQVRARIAEW
                     KTRLA"
     gene            2783..>3149
                     /locus_tag="VF686_07015"
     CDS             2783..>3149
                     /locus_tag="VF686_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8661600.1"
                     /translation="MKVSKAVLGLAAGVLLIGVIGGAALLYAKPGGIDKAAPPAAAKS
                     ELARFARGSIASLETPAALEPAPAYVFRTRDGADVRLADFRGKVVVVNVWAMWCLPCR
                     TEMPTLARLAEAYEGRDLVV"
BASE COUNT          456 a         1083 c         1138 g          472 t
ORIGIN      
        1 gaggtcgcca gcctcgagcg gcggcagcaa gcgatcgcgg gcgaagatgt cggtggactc
       61 gcagatcggc ccgacaaggt cataggccag aagctccccc tcgcgcggcc gcaccgggac
      121 cagatcgtgg aaggcgtcgt acagggccgg gcgcatcagg tcgttcatgc cggcgtccag
      181 cacgaggaag cggcggccat ccccgcgctg gttgacctgg atcacgcggc tcagcagcag
      241 gccggcgttg gcggccagca gccggccagg ttcgaaggcc gcctcgacat tgagtccgtc
      301 cagcacccgc gcgcacatgg cggcgtagtc ggcgatcgaa ggcgtgtccg tctggccgtg
      361 atacggcacg ccgagaccgc cgccgagatc gaggcggccg accgaatggc cgttggcgcg
      421 cagggtcagg accacgtcgc gcagggtgcg gaaggcggcc tccatcggct ccagcgtcgt
      481 gatctggctg ccgatatggc aggccagccc caccggcgtc agatgcggcg aggccgaggc
      541 gcgggcgtac aggtcgatcg cctcctcgac gggcacgccg aacttgtcgg tcgcgccgcc
      601 ggtcgtgatt ttcgcatgac cgcctgcccc gatcttcgga ttgacccgga tcgcgatggc
      661 gggggccttg ccctttgaag ccgccacctc gatcagccgg tccagctcgg caccgctctc
      721 gacattgatc tggcgcaccc ccgcctcgat ggcgaaggcc agttcggcgt cggtcttgcc
      781 cacgccggag aagatgatgc ggtcgccggg gatacccgcg gccagcgccc gccggatctc
      841 gccctcggac acggtgtcgg cgccgcagcc cagccgggcc agggtcgcca gcaccgacag
      901 gttggagttt gccttcaccg cgaaggcgat cagggcgtct ccgagcgcgt cccgatgcgc
      961 atccaccgcc tcgcgcagca ggctgtagtg ccgcgtcagg gtcgcggtcg aatagacata
     1021 ggcgggcgtg cccaccgcct cggcgagcgc ttccagcggc acgccctcgg cgtgaagcgc
     1081 gccgtcgatc cggtcgaaat ggtgcaaggc ctagcggttc ggctgggcgc ccgggccgtt
     1141 gtaggggctg ctcggacccc catcgatcgg ctgggtgctg ggcggacgat agacggtggc
     1201 gggctcgggc aggccggggg cgcgcgcgtc gcgctggctg cgctcggtct tcttggcggg
     1261 cggcgcctcg aggtccgcca tgcggccgca accggcggcg gagacggcga acagggcgag
     1321 gacgcccagg ctgacgatcg atttcatccc aatcccctac gcgagacgcg tcttccattc
     1381 ggcgatgcgc gcgcggacct gttccggcgc cgtccccccg aagctctggc ggctggcgca
     1441 ggaggcctca ggggtgagca ccttgtaaac cgcttccgtc acgcccgcct caagcgattg
     1501 cagttccgac agcggaagtt gcgacaaatc cacgcccagc gcctcggccc gcttcaccgc
     1561 cgcgcccgtg acatggtggg ccttgcggaa cggcaggttc agctcgcgca ccagccagtc
     1621 ggccagatcg gtggcggtcg agaagccggc gccggcggcg gcgcgcatcc gctctgtgtc
     1681 cggccgcagc gccgcgacca tggccgtcat ggcgcccaaa gccagcgtca gggcgtcgaa
     1741 ggcctcgaac accggcggct tgtcctcctg catgtccttc gaataggcca gcggcaggcc
     1801 cttcatcacc gtcgacaggg cgacgagcga gcccatgatc cgtccggtct tggcccgcac
     1861 cagttcggcg gcgtcggggt tgcgcttctg cggcatgatc gacgagcccg ttgtcaggtc
     1921 gtctggcagg ctggcgaagc cgaacatggg cgtcatccag atcacgatct cctcggccag
     1981 ccgcgacaga tggccggcgg cgatcgaggc ggccgccaga ctctccaggg cgaagtcgcg
     2041 gtccgagacg gcgtcgaggg aattggccat gggccggtcg aaacccagtt ccgaggccgt
     2101 catgtgccgg tcgatgggga agggcgaacc ggccagggcc gcggcgccca gcgggttctc
     2161 gttcatcctt tggcgcgcgt cggcgaagcg gccggcgtcg cggccgaaca tctcgacata
     2221 ggccatcaga tggtggccca gggtcaccgg ctgggcggtc tgcagatggg tgaagccggg
     2281 catcaggtcg ccggcatgct gttccgcccg cgccagcagg acggcctgca ggcctttcag
     2341 ctgttcgatc gcccggtcgc aggcgtcgcg gacccacagg cggaaatcgg tcgcaacctg
     2401 gtcgttgcgg ctgcgcgctg tgtgcagccg gcccgacggc tcgccgatca gctcggccag
     2461 ccgcgcctcg acgttcatgt ggatgtcctc gaactggtcg cggaacggga aggacccgtc
     2521 gcggatctcg ccctcgatgg cgtccagccc gcccagaatg gcgtccgcgt cggcccgggt
     2581 cacgatgccg cgcgccgcca gcatgcggga gtgggcccgc gaaccggcca gatcctgggc
     2641 ccacaggcgg cggtcgacgc cgatcgagac gttgatcgcc tgcatgatat cggcggggcg
     2701 cgccgagaag cgtccgcccc acatgtcttg accttccgcg ttcggatcgg ctttggtcgg
     2761 ggcgggcggc gttggatcgg acatgaaggt ctcgaaagcg gtgttgggct tggcggcggg
     2821 cgtgctgctg atcggcgtga tcggcggcgc cgcgctgcta tacgccaaac ccggcgggat
     2881 cgacaaagcg gcgccgccgg ccgccgcgaa aagcgagctg gcgcggttcg cccgcggctc
     2941 gatcgcctcg ctggaaaccc cggccgcgct ggagccggcg cccgcctatg tgttccggac
     3001 acgcgacggc gccgacgtcc ggctggccga cttccgcggc aaggtcgtgg tggtcaatgt
     3061 ctgggccatg tggtgcctgc cctgccgcac cgaaatgccg accttggccc gtctggccga
     3121 ggcctatgag ggtcgggacc tcgtcgtcc
//
LOCUS       DASVVN010000436         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_304744,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000436 DASVVN010000000
VERSION     DASVVN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304744"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF686_07020"
     CDS             <1..949
                     /locus_tag="VF686_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661601.1"
                     /translation="EQSVNPRDLFGGSTGFLPTEIKEQYVLPAGTLTWNFAEDMQFRL
                     GASKTLGRPQFRELAPQQYTDPESDRTFIGNPYLVDTEILNLDARWEWYFARQQYLTA
                     GVFYKDMDKPIESSVVDQGASISQTFLNAPNAVVMGVELEAKKYFEFASGMDFIANKR
                     WLVQGNYTWSDSQVNVEPGDTVTTQNSLGVAQPASFYVVDGSRLQGQSEHVANLQLGW
                     EDDTARSQATLIANYVSERTSARGRPGEPDLIQEPGISLDFVYRKDFDYQGRDYGFSL
                     ELRNLLGEEFLEYQELGTRVTINGYELGTSGSVSLTARF"
     gene            1204..2013
                     /locus_tag="VF686_07025"
     CDS             1204..2013
                     /locus_tag="VF686_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX8661602.1"
                     /translation="MRAGVEIALALALTVQLGQLIRIVVKPRAEVARAASTRPDVAPA
                     DYTVFQRFDAFFRTGDLGSLAEATAAGSSQMRLFGVRAGGAGGGSAIIGLADGRQVSV
                     GVGEEVEPGLILQSVGADHVTLARGGSVTRLIFSDVPVGAAPPPPPSPGPQTVGPDPD
                     PVVAAPPIGPAATTSPRVDPARLMAQASLRPRMQGLRVNGFTVSAAGDGAALKAAGLQ
                     SGDVILAVNGTAMDSLDRIAGLRSQLSNSTSAEIRFERGGAVQTATIRTGR"
BASE COUNT          328 a          720 c          669 g          309 t
ORIGIN      
        1 cgagcagtcg gtgaacccgc gggacctgtt cggcggctcg accggcttcc tgccgacgga
       61 gatcaaggag cagtacgtgc tgcccgccgg caccctgacc tggaatttcg ccgaggacat
      121 gcagttccgc ctcggcgcct ccaagaccct cggccggccc cagttccgcg aactggcgcc
      181 acagcagtac accgaccccg aaagcgaccg gaccttcatc ggcaacccct atctggtgga
      241 caccgaaatc ctgaatctgg acgcgcgctg ggagtggtat ttcgcccgcc agcagtatct
      301 gacggccggc gtcttctaca aggatatgga caagccgatc gagtccagcg tggtcgatca
      361 gggcgcctcg atctcccaga ccttcctgaa cgcgcccaac gccgtcgtca tgggcgtcga
      421 gctggaggcg aagaagtatt tcgagttcgc ctcgggcatg gacttcatcg ccaacaagcg
      481 ctggctggtc cagggcaact acacctggtc ggactcccag gtgaacgtcg agccgggcga
      541 caccgtcacc acccagaaca gcctgggcgt ggcgcagccg gcgtcgttct atgtcgtcga
      601 cggcagccgc ctgcagggcc agtccgaaca cgtcgccaac cttcagctgg gctgggaaga
      661 cgacaccgcc cggtcgcagg cgaccctgat cgccaactat gtatctgaac gcacctctgc
      721 gcggggccgt ccgggcgagc ccgacctgat ccaggagccg ggcatctcgc tggacttcgt
      781 ctaccgcaag gacttcgact atcagggccg cgactacggc ttctcgctcg aactgcgcaa
      841 cctgctgggc gaggagttcc tggagtacca ggagctgggc acccgcgtga ccatcaacgg
      901 ctacgaactg ggaaccagcg gctcggtcag cctgaccgcg cgcttctaga ccgcgaacgg
      961 ggaggccgtc cgaccgcctc ccccgaaccg ctgcacgacc gttttgcggc ggttcatccg
     1021 tgtgcgcacc aaccgtcacg gacagttctc cgggccgtca tgtggtggtg aagccggacc
     1081 cggacgattg ggctacggag tgggccgggc cgtcggggac ccgtggggca cgccatagtg
     1141 atcggcgcac tgaacaactt cagcagcgga gttcgggacg ccgtttcagc gcgtccggat
     1201 cgcgtgcgcg ccggtgtcga gatcgccctc gccctggccc tgacggtgca gctgggccag
     1261 ctgatccgga tcgtcgtcaa gccgcgggcg gaagtcgcga gggcggcctc gacgcgccct
     1321 gacgtcgccc ccgccgacta cacggtcttc cagcgtttcg acgccttctt ccgcaccggc
     1381 gacctgggca gccttgcgga agccacggcc gcgggcagca gccagatgcg cctgttcggc
     1441 gtgcgcgcgg gcggcgccgg cggcggttcc gctatcatcg gcctggccga cggacgtcag
     1501 gtgtcggtag gcgtgggaga ggaggtcgag cccggtctga tcctccagtc ggtcggcgca
     1561 gaccatgtca ccctcgcgcg cggcggctcg gtcacccgcc tgatcttcag cgacgttccg
     1621 gtgggcgccg ctccgccgcc tccgccgtcg cccgggccgc agaccgtcgg tccggatccc
     1681 gatccggtcg tcgccgcgcc tccgatcggt cccgccgcga caaccagccc ccgcgtggac
     1741 cccgcccggc tgatggcgca ggcctcgctg cggccaagga tgcaggggct gcgggtcaat
     1801 ggtttcaccg tctccgccgc aggcgacggc gccgccctga aggccgcggg cctccagtcg
     1861 ggcgacgtca tcctggccgt gaacggaacc gcgatggaca gtctggaccg catcgccggc
     1921 ctgcgcagcc agctctcgaa cagcacaagc gccgagatcc gcttcgaacg cggcggcgca
     1981 gtgcagaccg ccaccataag gacaggccgt tgaagcgatc gaaccg
//
LOCUS       DASVVN010000437         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_252936,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000437 DASVVN010000000
VERSION     DASVVN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252936"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VF686_07030"
     CDS             <1..1365
                     /locus_tag="VF686_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661603.1"
                     /translation="LHANGSEEQKRTYLPKLVSGEWGGTMNLTEPQCGTDLGLIRTKA
                     VPDGDGSYRISGQKIWISSGEHDFTDNIIHLVLARIEGAPAGVKGISLFVVPKHFVSD
                     DGSLGERNNARCAGLEHKMGIHGNATAVMVYEDAMGWLVGEENRGLQAMFVMMNEARL
                     GTGLQGLAIGAAAYQAGVEFAKERLQGRSLTGPKNPEGPADPIIVHPDVRRMLMESKA
                     FVEGGRAFLLWTALQADLQKAPDDALRTKAQDYMGLLTPVLKAYLTDRGFHVASQAMQ
                     VHGGSGYTEHFPASQYLRDARITMIYEGANGIQALDLVGRKLAANGGRGVMTFFAEID
                     AFVAEIEGDEALKPYADGLRSAKGQLQEATMWLMQNGLQNPDNAGAASTDYLHLFGLT
                     MLAYMWALMARASAPHAGDPFHDAKLTTARYFVERVLPDTAGHLAKLKTGAATMMALP
                     VEAF"
BASE COUNT          265 a          508 c          535 g          203 t
ORIGIN      
        1 ctgcacgcca acggctccga ggagcagaag cggacctacc tgcccaagct ggtctccggc
       61 gagtggggcg ggacgatgaa cctgacggag ccgcaatgcg gcaccgacct ggggctgatc
      121 cgcaccaagg ccgtgccgga cggcgacggg agctaccgga tcagcggaca gaagatctgg
      181 atcagctcgg gcgagcacga cttcaccgac aacatcatcc acctggtgct tgcgcgcatc
      241 gagggcgctc ccgccggcgt gaagggcatc agcctgttcg tggtgccgaa gcacttcgtg
      301 agcgatgacg gatccctggg cgagcgcaac aacgcgcgct gcgccggcct cgagcacaag
      361 atgggcatcc acggcaacgc caccgccgtg atggtctacg aggacgccat gggctggctg
      421 gtcggcgaag agaaccgcgg gctgcaggcc atgttcgtga tgatgaacga ggcgcggctc
      481 ggcaccgggc tgcagggcct ggccatcggt gcggccgcct accaggccgg cgtggagttc
      541 gccaaggagc ggctgcaggg gcgctcgctc accggcccga agaatccgga agggccggcc
      601 gatccgatca tcgtgcaccc ggacgtgcgg cgcatgctga tggagtcgaa agcgttcgtg
      661 gaaggcggcc gcgccttcct gctgtggacc gcgctgcagg ccgacctgca gaaggcgcct
      721 gacgacgcgc tgcgcaccaa ggcgcaggac tacatgggcc tgctgacgcc ggtgctgaag
      781 gcgtacctga cggaccgtgg cttccatgtc gccagccagg cgatgcaggt gcacggcggc
      841 tcgggctaca ccgagcactt ccctgcctcg cagtacctgc gcgacgcgcg catcaccatg
      901 atctacgaag gcgccaacgg catccaggcg ctcgacctgg tcgggcgcaa gctggcggcc
      961 aacggcggcc gcggcgtgat gaccttcttc gccgagatcg acgccttcgt ggccgagatc
     1021 gagggcgacg aggcgctgaa gccctacgcc gacggcctgc ggagcgcgaa gggccagctg
     1081 caggaggcca ccatgtggct gatgcagaac ggactgcaga accccgacaa cgccggcgcc
     1141 gcctcgaccg actacctgca cctcttcggt ctgaccatgc tggcctacat gtgggcgttg
     1201 atggcgcggg cctccgcgcc gcatgcgggc gatccgttcc acgacgccaa gctgaccacc
     1261 gcgcgctact tcgtcgagcg cgtgctgccg gacacggcgg gccacctcgc caagctgaag
     1321 acgggagcgg cgaccatgat ggcgctgccc gtcgaggcgt tctgatgggg gcgctggacc
     1381 tgcccaaggc gaagtggcgc gacgaagagg acctcaacat ctttgaggac gccaccgcgc
     1441 gcttcctcga ggagcacgcg ccgggcgagg cctccctgcg ctggcgcgag gagggcatgg
     1501 tcccccgcca g
//
LOCUS       DASVVN010000438         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_362477,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000438 DASVVN010000000
VERSION     DASVVN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362477"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VF686_07035"
     CDS             complement(<1..1662)
                     /locus_tag="VF686_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661604.1"
                     /translation="MRLSRLATLLTGCAVLALTAPAFAQDAPVIIDPPLRSPVPQSPV
                     VVPLSGADAVAPEELHPPLVVPDEESAPEAVEPPAPPIPAIWAPVPVDAAGQSAYGLY
                     LAGRLASIRGDRAAGSAFLGDAHALAPEQPALGEEAFRSGLFSGDLGDVARLTPTVAG
                     SPDLAEAGRLIELVQALRSGQTRQSLALLNQRPFAEPFSAAARYLTPAVAGAAGDWDL
                     ALKAVEAAATDPAGLILHQQRAGLLESRRRFAEADAEYKILMAAPGGAMLYALDYAEF
                     LERQGRRDEALAIYDAALTGGNPNSEAVLGRARIVGRGRPPAEPNALERAADALEFAA
                     LQTSANQAWELSAIYLRMADSLHPDDLISLQLGQALANGRQPLLAREAFVRVSQSDPI
                     TYARAQVGLAGALDAQGDTEGALEALRRADAAAVDQAAFAYTLAAHLGGMGRHEEALA
                     VLNRPTINVAAQPAHFRFLRGTVLENLNRIDEAEAELWAALQAQPNDPTILNYLGYMW
                     VDSGRRVAEGAEMLARAHAAEPQNGNIQDSLGWAQFRQGQYEIAVQ"
     gene            complement(1718..>2241)
                     /locus_tag="VF686_07040"
     CDS             complement(1718..>2241)
                     /locus_tag="VF686_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8661605.1"
                     /translation="LKRVRNAKPLLSRLGTSLGGAVGLFDMWCQTLFGGLSLFGTLKH
                     SKTDAEATEKASKHRPIAYPKPDGKLSFDKLSSVFISNTNHAEEQPAHLKLLDPSIPI
                     RVNLPEYGEPARLYCPAGVYEVLYDEQGNNPRFQINAQNCVHCKTCDIKDPSQNIVWT
                     TPEGGGGPNYPNM"
BASE COUNT          345 a          736 c          812 g          348 t
ORIGIN      
        1 ctgaacggcg atctcgtact ggccttgccg gaactgggcc cagccgagcg agtcctggat
       61 gttgccgttc tggggctcgg ccgcatgggc gcgggccagc atctcggcgc cttcggccac
      121 gcggcggccg ctgtcgaccc acatgtagcc aaggtagttg aggatggtcg ggtcgttggg
      181 ctgggcctgc agggcggccc acagttcggc ctcggcctcg tcgatccggt tcaggttttc
      241 cagcaccgtg ccgcgcagga agcggaaatg cgccggctgg gccgcgacat tgatcgtcgg
      301 ccggttgagg acggccagcg cctcctcgtg ccgacccatc ccgcccagat gcgcggccag
      361 cgtataggcg aaggccgcct gatcgaccgc ggcggcgtcc gcgcggcgaa gcgcctcaag
      421 cgcaccttcg gtgtcgcctt gggcatccag cgcccccgcc aggccgacct gggcccgggc
      481 gtaggtgatg ggatcgctct ggctgacccg gacaaaggcc tcgcgtgcaa gcaggggctg
      541 gcggccattg gcgagagcct gccccagctg aagactgatc aggtcatcgg gatgcaggct
      601 gtccgccatt cgcagataga tcgccgacag ttcccacgcc tggttcgcgc tggtctgcag
      661 ggcggcgaac tccagtgcgt cggccgcacg ttccagcgca ttcggctcag ccggagggcg
      721 gccgcgtccc acgatgcggg cccggcccag gaccgcctcc gaattgggat ttccgccggt
      781 gagggcggcg tcatatatgg ccagcgcctc gtcccggcgg ccctgccgct cgaggaattc
      841 ggcgtagtcc agcgcgtaca gcatggctcc gccgggagcg gccatcagaa tcttgtattc
      901 ggcgtcggct tcggcgaagc ggcggcggct ttccagcagg ccggcgcgct gctgatgcag
      961 gatcaggcct gccggatcgg tcgccgccgc ttcgaccgcc ttcagcgcca gatcccagtc
     1021 ccccgccgca ccggcgacgg cgggcgtgag gtaacgggcc gccgcgctga acggctcggc
     1081 gaacgggcgc tggttcaaca gggccagact ctgacgcgtt tggccggaac gcagcgcctg
     1141 aaccagctca atcaaccggc cggcctcggc gagatcaggg ctgcccgcga cggtgggcgt
     1201 cagacgggcc acatcgccaa ggtcgccgga gaaaagcccg gagcggaagg cctcttcgcc
     1261 aagagcaggc tgttcgggcg ccaaggcgtg ggcgtcgcca aggaaagccg agccagccgc
     1321 ccggtcgccc cggatgctgg ccagacggcc ggcgagatag aggccgtagg cggactggcc
     1381 cgcagcgtcg accggcacgg gcgcccagat cgcggggatc ggcggcgccg gcggctcaac
     1441 ggcctcgggc gccgactcct cgtcggggac caccagcggc ggatggagct cttccggcgc
     1501 cacggcgtct gcgccggaaa gggggacgac gaccggtgac tgcgggaccg gggacctgag
     1561 cggcgggtcg atgatgaccg gcgcgtcctg tgcgaacgca ggggccgtca gggcaaggac
     1621 ggcgcaaccg gtcaggagag tagcgaggcg ggaaaggcgc atgacggaac cctcaccgga
     1681 ttatgcggcg acgacaaggc ccctcgcctg ccggatgtca catattcgga tagttcgggc
     1741 cgccgccgcc ctcgggcgtg gtccagacga tgttctgcga cgggtccttg atgtcgcagg
     1801 ttttgcagtg gacgcagttc tgggcgttga tctggaagcg ggggttgttc ccctgctcgt
     1861 cgtacagaac ctcgtagacg cccgccgggc agtagagccg cgccggctcg ccgtactcag
     1921 gcaggttcac ccggatgggg atggacgggt ccagcagttt caggtgcgcc ggctgttcct
     1981 cggcatggtt ggtgttggaa atgaagaccg acgacagctt gtcgaacgac agctttccat
     2041 ccggcttggg ataggcgatc ggcctgtgct tcgaggcctt ctccgtcgct tccgcatcag
     2101 tcttgctgtg cttcagcgtg ccgaacagcg acaggccgcc gaacagggtc tggcaccaca
     2161 tgtcgaacag gccgaccgcc ccgcccagcg aggtgccgag cctggatagc aggggtttgg
     2221 cgttccggac gcgtttgagc t
//
LOCUS       DASVVN010000439         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_175712,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000439 DASVVN010000000
VERSION     DASVVN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175712"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VF686_07045"
     CDS             <1..992
                     /locus_tag="VF686_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8661606.1"
                     /translation="GVHFRDGDYVRRGQLLFTLDPRPAQAALASARAQLAQAQAQISL
                     AQSELTRSETLLASQAVSQAEVDTRRGALMTARAAIAAANANVRARQLDLEFTRVTAP
                     ISGRVSDRRVDAGNLVAGGSSAADVLTTVVSSAPIHFVFDGSEAVLLKYQRQARRGAA
                     PIQVRLQDEGDFSRSGTLDFTDNAVDTASGVIRLRALVPNADGFLKPGMFGQARLAGA
                     GTYNAMLVPDASVATDQARRIVYVVARDGSVAPRPVQLGPLVDGLRVVRSGLTATDRV
                     IINGVQRIQAPGMKVQAKLGQIRPAAAQVSQAPVTTAAPASSATFANSLSSVD"
     gene            1000..4260
                     /locus_tag="VF686_07050"
     CDS             1000..4260
                     /locus_tag="VF686_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX8661607.1"
                     /translation="MNISRFFIDRPIFAAVISVFITLLGVFAYPLLPLSQYPEIAPPT
                     IAVTAVYPGASAETMAETVAAPIEQEVNGVERMLYISSSSTSDGVATITVTFEPGTDL
                     DAAQVLVQNRVALAEPRLPEPVRQIGVTVNKQQQDFLLIIGLTSPDRSLANDYIGNYA
                     NSVIRDRMLRLDGVGAVQVFGGGNYSMRVWIDPAKAAARNLTAQEIVGALRGQNSSAA
                     AGAIGQPPFPTNAAAFQLPVQVQSRLSDPDQFGDIVIKTDTQGRTTRVRDVARVELGS
                     QDYGIRGYFDGQPGVALAVQQQPGANALETAELVQAEIAAIRAELPQGMAISVPYNPT
                     EFVAVSVEAVQHTVFEAMLLVVLVVIVFLQTWRAAIIPIVAIPVSLVGTFAVLLALGY
                     SINSLSLFGLVLAIGIVVDDAIVVVENVERYIRMGLTPKEAAYRSMKEVSGALIAIGL
                     VLVAVFVPTAFVPGIPGIFYQQFAVTIASASVISLLVSLTLSPAMAAVLLKPHDLSHA
                     PTRKPGVVGTVAYSGGWAGRRFNDGFDWLSDKYGRLTARLVRTVGLVLAIYVGLLALT
                     AWRVVDTPTGFIPDQDQGYLIGVIQLPPGSSLERTEAVMSQARNIIEKTEGVDGTVAF
                     AGLDGTSFSFGSNAATIFVRLDEFDHRNAEQSAVALAGAITGATFGIEDANIFVIAPP
                     AVQGLGNGNGFQMMVQDRGGAGYRGLEGATFAMMGASAQAPTQVQQVFSTYNTGSPRV
                     SADVDRDKALMMGVQPADVFNTLGIYLGSSYINDFNFLGRTFRVTAQAEPASRDDVAD
                     IANLKTRSASGAMVPIGSVATLRDDSGPARVVRYNLFPASELQGQAAPGVSSGQALAA
                     MEGIAAQALPAGFSYEWTGLALQEKSSSGGTTLVFLMAVIFVFLVLAAQYEAFTLPTA
                     VVLIVPMCVLAAMIGIGIRGLDNNILVQIGLVVLIALAAKNAILIVEFAKQAEEEEGL
                     SRWDAAVKAARIRLRPILMTSFAFIFGVVPLMLATGPGAEMRQSLGTAVFSGMLGVTL
                     FGLIFTPVFYVVSRWAASKIPQSPKKDPTVPTTDGFPAHDQPASEALPAAPRLGDA"
     gene            4260..>5344
                     /locus_tag="VF686_07055"
     CDS             4260..>5344
                     /locus_tag="VF686_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8661608.1"
                     /translation="MIAQPSSRILTATAFASLALASCAVGPRAPDATLPPSASGAFVG
                     AATPAVTAEAARDDWWRLYDDPTLDGLILQALTENNELEAAAANLRRVRAALGEARVG
                     RLPSTTVGATGQYGRASAATVPGGNGGQPLDEGETYDVGLDVAYEIDLFGRVGSSIRA
                     ARADADAAAAALDVVRVSVAAETARAYADACSANAQIAVAERTIELQSNTADLTQRLL
                     AAGSGNGLDTSRARAALESTRASLPPLRAQRDGALFRLATLTGRTPAEASNAARACVR
                     PPQLTRAIPIGDGAQLLARRPDVRRAERDLAAAAARVNVATADLYPRISLGGSVGSTA
                     LDAGDLTNDSSFRFSVGPLISWSFPNV"
BASE COUNT          740 a         1978 c         1761 g          865 t
ORIGIN      
        1 cgggcgtcca tttccgcgac ggcgactatg tgcgccgcgg gcagctgctg ttcaccctcg
       61 acccgcgccc ggctcaggcg gcgctcgcct cggcccgcgc ccagctggcc caggcccagg
      121 cgcagatcag cctggcccag agcgaactga cccggtccga gaccctgctg gcttcgcagg
      181 cggtttcgca ggccgaggtc gacacccgtc gcggcgccct gatgacggcc cgcgccgcca
      241 tcgccgccgc caacgccaat gtccgcgccc gtcagctgga tctcgagttc acccgcgtca
      301 ccgcccccat ctcgggccgc gtctcggacc gccgggtcga tgcgggcaat ctggtcgccg
      361 ggggctcttc ggccgccgac gtgctgacca ccgtggtgtc cagcgcgccg atccatttcg
      421 tcttcgacgg ctccgaagcg gtgctgctga aataccagcg gcaggcccgc cgtggcgccg
      481 cgccgatcca ggtgcggctg caggacgagg gcgacttcag ccgttcgggc acgctcgatt
      541 tcaccgacaa cgccgtggac accgcctcgg gcgtgatccg gctgcgagcg ctggtgccca
      601 acgccgacgg cttcctgaag cccggcatgt tcggtcaggc ccgtctggcc ggcgccggaa
      661 cctacaacgc catgctggtg cccgacgcga gcgtcgccac cgatcaggcc cggcgcatcg
      721 tctatgtggt cgcacgcgac ggctccgtcg cgccgcgtcc ggtgcagctg gggccgctgg
      781 tcgacgggct gagggtggtc cgttcgggcc tcacggccac cgaccgcgtc atcatcaacg
      841 gcgtccagcg catccaggcg ccgggcatga aggtgcaggc caagctggga cagatccgtc
      901 cggccgcggc acaggtatcg caggcccctg taaccaccgc ggcgccggcc tccagcgcca
      961 ccttcgccaa cagcctgtcg tcggtcgact gatccggtca tgaacatctc acgcttcttc
     1021 atcgaccggc cgatcttcgc ggccgtgatc tcggtgttca tcacccttct gggcgtcttc
     1081 gcctatccgc tgctgccgct gtcgcagtat ccggagatcg cgccgccgac gatcgcggtc
     1141 acggccgtct atccgggcgc ctcggccgaa accatggccg agaccgtggc cgcgccgatc
     1201 gagcaggagg tcaacggcgt cgagcgaatg ctctacatct cgtcctcctc gacctcggac
     1261 ggggtggcga ccatcaccgt caccttcgaa ccgggcacgg atctggatgc ggcccaggtg
     1321 ctggtccaga accgggtcgc cctggccgag ccccgcctgc ccgaaccggt gcgccagatc
     1381 ggcgtgacgg tgaacaagca gcagcaggac ttcctgctga tcatcggcct gacctcgccc
     1441 gaccgcagtc tggccaacga ctatatcggc aactacgcca actccgtgat ccgcgaccgg
     1501 atgctgcgcc tcgacggcgt cggcgcggtc caggtgttcg gcggcggcaa ctattcgatg
     1561 cgggtctgga tcgatcccgc caaggccgcc gcacggaatc tgacggccca ggaaatcgtc
     1621 ggcgccctgc gcggacagaa cagctccgcg gccgccggcg ccatcggcca gccgcccttc
     1681 cccaccaacg ccgcggcctt ccagctgccg gtgcaggtgc agtcgcgcct gagcgatccc
     1741 gaccagtttg gcgacatcgt catcaagacc gacacacaag gccgcacgac ccgggtccgt
     1801 gacgtcgcgc gggttgaact gggttcgcag gactacggca tccggggcta tttcgacggc
     1861 cagccgggcg tcgccctcgc cgtccagcaa cagcccggcg ccaatgcgct tgagacggcg
     1921 gaactggtcc aggccgagat cgcggccatt cgcgccgaac tgccccaggg catggcgatc
     1981 tcggtgccct acaaccccac cgaattcgtg gctgtatccg tcgaggccgt gcagcacacg
     2041 gtgttcgagg ccatgctgct ggtggtgctg gtggtcatcg tcttcctgca gacgtggcgc
     2101 gcggcgatca tcccgatcgt ggctatcccc gtctcgctgg tcggcacctt cgccgtgctt
     2161 ctggcgctcg gctattcgat caactcgctg tcgctgttcg gcctggttct ggccatcggc
     2221 atcgtcgtcg atgacgccat cgtcgtggtc gagaacgtcg agcggtatat ccgcatgggc
     2281 ctgacgccca aggaggccgc ctaccggtcg atgaaggaag tgtccggggc gctgatcgcc
     2341 atcggcctgg tgctggtggc ggtgttcgtg ccgaccgcct tcgtgcccgg catccccggc
     2401 atcttctacc agcagttcgc cgtgaccatc gcctcggcct cggtcatctc cctgctggtg
     2461 tcgctgaccc tgtcgccggc catggccgcg gtgctgctga agccgcacga cctgagccac
     2521 gccccgaccc gcaagccggg cgtggtgggc acggtcgcct actctggcgg ctgggccgga
     2581 cggcgcttca acgacggctt cgactggctg tcggacaagt acggccggct gaccgcgcgt
     2641 ctggtgcgga cggtgggtct ggtgctggcc atctatgtcg gcctgctggc cctgaccgca
     2701 tggcgggtcg tcgacacccc gaccggcttc atcccggacc aggatcaagg ctatctgatc
     2761 ggcgtcatcc agctgccgcc cggctcgtcg ctggaacgca ccgaggcggt gatgagccaa
     2821 gcccggaaca tcatcgaaaa gaccgagggc gtggacggca cggtggcctt cgccggcctc
     2881 gacggcacca gcttctcgtt cggctccaac gccgcgacca tcttcgtgcg tctggacgag
     2941 ttcgaccacc gcaacgccga gcaatcggcc gtcgccctgg ccggagccat caccggcgcc
     3001 accttcggca tcgaggacgc caacatcttc gtcatcgccc cgcccgcggt tcagggtctg
     3061 ggcaatggca acggcttcca gatgatggtt caggaccgcg gcggcgccgg ctatcgcggc
     3121 ctcgagggcg ccaccttcgc catgatgggc gcgtcggccc aggcgccgac gcaggtgcag
     3181 caggtgttct cgacctacaa caccggttcg ccccgcgttt cggccgacgt cgaccgtgac
     3241 aaggccctga tgatgggggt ccagccggca gatgtgttca acacgctcgg catctatctg
     3301 gggtcgtcct acatcaacga cttcaacttc ctgggccgca ccttccgggt cacggcgcag
     3361 gcggagccgg cctctcgcga cgacgtcgcc gacatcgcca atctgaagac ccgctcggcc
     3421 tccggcgcca tggtgccgat cgggtcggtg gcgaccttga gggacgattc cggcccggcc
     3481 cgcgtggtcc gctacaacct gttcccggcg tccgagcttc agggacaggc ggcgccgggc
     3541 gtgtcgtccg gccaggcgct ggccgccatg gaaggcatcg ccgcccaggc cttgcccgcc
     3601 ggcttctcct acgaatggac ggggctggcg ctgcaggaga aatcgtcctc gggcgggacc
     3661 accctggtct tcctgatggc ggtgatcttc gtcttcctgg tgctggccgc ccagtatgag
     3721 gccttcacgc ttccgaccgc cgtggtgctg atcgtgccga tgtgcgtgct ggcggcgatg
     3781 atcggcatcg gcatccgcgg tctggacaac aacatcctgg tccagatcgg actggtggtg
     3841 ctgatcgcgc tggcggccaa gaacgccatc ctgatcgtgg agttcgccaa acaggccgag
     3901 gaggaagagg ggctgagccg ctgggatgcg gccgtcaagg ccgcgcgcat ccgtctgcgt
     3961 ccgattctga tgacctcgtt cgccttcatc ttcggcgtcg tgccgctgat gctggcgacc
     4021 ggcccgggcg ccgagatgcg tcagtcgctg ggcaccgccg tcttctccgg catgctgggc
     4081 gtgaccctgt tcggcctgat cttcaccccg gtcttctatg tggtcagccg ctgggccgcc
     4141 tcgaagatcc cgcagtcgcc gaagaaggac ccaaccgtcc cgaccaccga cggcttcccc
     4201 gctcacgacc agcccgcgtc cgaggctctg cccgccgcgc cgcgcctggg agacgcctga
     4261 tgattgccca accctcttcc cggatcctga ccgcaactgc cttcgcctcg cttgcgctgg
     4321 cgtcgtgcgc ggtcgggccg agggcgcccg acgctaccct tcccccctcg gcgtcgggcg
     4381 ccttcgtggg cgccgccacc cccgccgtga ccgccgaggc ggcgcgcgac gactggtggc
     4441 ggctctatga cgaccccacc ctcgacggcc tgatcctcca ggccctgacc gagaacaacg
     4501 agctggaggc cgccgcggcc aacctgcgcc gcgtccgcgc ggccttgggc gaagcccggg
     4561 tcggacggct gccgtccacc actgtcggcg ccacgggtca gtacggccgc gcctcggccg
     4621 ccacggttcc cggcggcaat ggcggacagc cgctggacga gggcgagacc tatgacgtcg
     4681 gcctggacgt cgcctatgag atcgacctgt tcggccgggt cggcagctcg atccgcgcgg
     4741 cccgcgccga cgccgatgcc gccgcggcgg cgctggacgt ggtgcgggtg tcggtcgcgg
     4801 cggaaacggc ccgcgcctat gccgacgcct gttcggccaa cgcccagatc gcggtcgccg
     4861 aacggaccat cgagctgcaa tcgaacaccg ccgacctgac ccagcgtctg ctggccgccg
     4921 gctccggcaa cgggctcgac acgtcgcgcg cccgcgccgc tctggagagc acccgcgcct
     4981 cgttgccgcc cctgcgcgcc cagcgggacg gggccctgtt ccggctggcg accctgaccg
     5041 gccgcacgcc cgccgaggcc tcgaacgcgg cccgcgcctg cgtccgcccg ccccagctga
     5101 cccgggccat cccgatcggc gacggcgcgc aactgctggc ccggcgcccc gacgtgcgcc
     5161 gggccgagcg cgatctggcc gccgccgcgg cgcgggtgaa cgtcgccact gcggatctgt
     5221 atccgcgcat cagcctgggc ggttcggtgg gatcgacggc gctggacgcc ggcgacctga
     5281 ccaacgactc cagcttccgc ttcagcgtcg ggccgctgat cagctggtcc ttccccaacg
     5341 tctt
//
LOCUS       DASVVN010000440         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_282046,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000440 DASVVN010000000
VERSION     DASVVN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282046"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..766
                     /locus_tag="VF686_07060"
     CDS             113..766
                     /locus_tag="VF686_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661609.1"
                     /translation="MNPAPLSSPALFPERPAPLEEACAASPRDGAALLAFALSRRLEG
                     DGRPVLMTATRAWLGEHGRPYGPGLPGTSLILAPVNTAAEALWTLEQALRSGAVSLAL
                     GTVDAATLAQTRRLDFAAKHGGCVGLVLPRGLDGLSAARRRWRISTLASAVDPDDAKG
                     PGAARLTAELVRGRGERPGAWMLEQDDETHRLRLADRLADSGLAAIGHAGAPSGLAA"
     gene            672..2279
                     /locus_tag="VF686_07065"
     CDS             672..2279
                     /locus_tag="VF686_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX8661610.1"
                     /translation="MRRIVSVWLIDWPIAVWRRSAMRARRPASPPDPALDPKNPFALI
                     LKNSRGAAVIHALNPAARAAGLRRGQTQADALAMIPHLICKPADIEADARALTALAVW
                     AERWSPSVSLDPAGEGLEGLFLDVTGATHLFGGEPALLTQIRDRLAEAGVRARAAMAP
                     TPGAAWALARWGEDKGGGCIADEDDARGLLAYLPVEALRIDDRTLTQARRFGLKRIGD
                     LYPMPRAGLARRFRDGAGVGLVQRLDQALGFAGEALTPVRPPPKYRAWQAWMEPVGDV
                     EGVEARLTDLTADLSAPLMRDGQGAKALTLTGFRSDGRTTHLSVRMGRPGRDAGVWMR
                     LFREAGLGRLELGFGLDAMMLTADAVEAMTARQGALESEAEAKQAESLAALIDRLTAR
                     LGEDRVLTAEPVDSWLPERAERWRPALGRSPSAAEGDACRPILLLDPPEPVEALAELP
                     DGAPVRFTWRRLSRRVTRSDGPERLSPEWWRPRPDDRQVRTRDYYRVEDEAGGRYWLF
                     REGLYGREYSGAAEEHAPSWWMHGVLP"
BASE COUNT          298 a          736 c          924 g          324 t
ORIGIN      
        1 ctgaaggcgc ggccccgggt ctgaattccg atcggggagg cggaacggga ttctctgttt
       61 gactctgacg gttgttggaa caaatagtga acatctggat ttccggactc acatgaaccc
      121 cgcgcccctg tcatctcccg ccctgtttcc cgaacggccc gcgccgctgg aggaggcctg
      181 cgcggcgagt cctcgggacg gagcggcctt gctggccttc gccctgtcgc ggcggctgga
      241 gggggacggg cggccggtgc tgatgacagc aacgcgggcg tggctggggg agcatggccg
      301 tccctatggc cccggtttgc cgggaacgtc cctgatcctc gcgccggtga acacggcggc
      361 cgaggccttg tggacgctgg aacaggcgtt gcggtccgga gcggtgtcgc tggcgctcgg
      421 cacggtggat gcggcgacct tggcccagac ccggaggctg gatttcgcgg ccaaacacgg
      481 gggatgcgtg ggtctggtgc tgccgcgcgg gctggacggg cttagcgccg cgcgccggcg
      541 ctggcggatc tcgaccctgg ccagcgccgt cgatcccgac gacgccaagg ggccgggagc
      601 ggcgcggctg acggccgaac tggtgcgcgg gcgcggggag cggccggggg cgtggatgct
      661 ggagcaggat gatgagacgc atcgtctccg tctggctgat cgactggccg atagcggtct
      721 ggcggcgatc ggccatgcgg gcgcgccgtc cggcctcgcc gcctgatccc gcgctcgacc
      781 cgaagaaccc gttcgccctg atcctgaaga acagccgggg cgcggcggtg atccacgccc
      841 tgaatccggc cgcccgcgca gcgggcctga gacgcggcca gacgcaggcc gacgccctgg
      901 ccatgatccc gcatctgatc tgcaagcccg ccgatatcga ggccgacgcc cgggccctga
      961 cggcgctggc ggtctgggcc gagcgctggt cgccctcggt cagcctcgat ccggcggggg
     1021 aaggattgga ggggctgttc ctcgacgtca cgggcgcgac ccatctgttc ggcggggagc
     1081 cggcgttgct gacccagatc cgtgaccggc tggccgaggc cggggtgcgg gcgcgggccg
     1141 cgatggcgcc gacgccgggg gctgcatggg ccctggcgcg atggggagag gacaagggcg
     1201 gcggctgtat cgccgacgag gatgatgcgc gcggtctgct ggcctatctg ccggtcgagg
     1261 cgctgcgcat cgacgatcgg accctgacgc aggcgcggcg gttcgggctg aagcggatcg
     1321 gcgaccttta ccccatgccg cgagcggggc tggcgcggcg gttccgggac ggggccggcg
     1381 tcggtctggt gcagcggctg gatcaggcgc tgggcttcgc gggcgaggcc ctgacgccgg
     1441 tgcgtccgcc gccgaaatac cgggcctggc aggcgtggat ggaaccggtc ggcgacgtcg
     1501 agggggtgga agcgcggttg acggacctga ccgccgacct gtccgcgccc ctgatgcgcg
     1561 acgggcaggg ggcgaaggcc ctgaccctga cgggtttccg ttcggacggc cggacgacgc
     1621 acctgtcggt gcggatgggc cgacccggac gggacgcggg cgtctggatg cggctgttcc
     1681 gcgaggcggg actggggcgg ctggagctgg gcttcggcct cgacgccatg atgctgaccg
     1741 ccgacgcggt cgaggcgatg acggcgcggc agggggcgct ggaaagcgag gccgaggcga
     1801 aacaggccga gagtctggcc gccctgatcg accgcctgac cgcgcgtctg ggcgaagacc
     1861 gggtgctgac ggccgagccg gtggacagct ggctgcccga gcgcgccgaa cgctggcggc
     1921 cggcgctggg acggtcgccg tccgcggcgg agggcgacgc ctgtcggccc atcctgctgc
     1981 tggatccgcc cgagccagtc gaggccctgg ccgaactgcc tgacggggcg ccggtgcggt
     2041 tcacatggcg tcggctgtcg cggcgggtga cccgttccga cggtcccgaa cggctgtcgc
     2101 ccgaatggtg gcggccacgc cccgacgacc ggcaggtgcg cacccgcgac tactatcgcg
     2161 tcgaggacga ggcgggcggc cgatactggc tgttccggga gggtctgtac gggcgggaat
     2221 attcgggcgc ggcggaagaa cacgcaccct cgtggtggat gcacggggtg ctgccatgac
     2281 gg
//
LOCUS       DASVVN010000441         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_387274,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000441 DASVVN010000000
VERSION     DASVVN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387274"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1530)
                     /locus_tag="VF686_07070"
     CDS             complement(160..1530)
                     /locus_tag="VF686_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661611.1"
                     /translation="MSPHLVATSSQTGGKRIAVVGSGISGLSCAWLLSQSHDVTLFEA
                     DGRIGGHSHTVDAPAPSEPVAVDTGFIVYNQDNYPNLTALFEHLDVPTKPAMMSFAVS
                     IDDGAFEYCSQGLGALFAQKRNYASPKFWRMIGDLLRFQKQAPRDLPELERSGETLEA
                     YLTRGGYGPLFREAHLLPQAAAIWSCTMGQMASYPAASFVRFYMNHNLLKVDLRPTWR
                     TVDGGSRSYVNRLTAAFKGRIVTNAGIAGVLRGLDGAALRFVDGRLERFDAVVLASHS
                     DQALALLEAPSADDRRLLGAIEYRPNKVILHRDTSLMPKRRKAWAAWTHLGYSDRAGE
                     GGVTYWMNELQSLPGPPLFVSLNPAKTPDPELVIGEWDYEHPVFDAAAVAAQSELWSL
                     QGRRNVWFAGAWFGSGFHEDGLQAGLAVAEQLGGVRRPWSVAGESGRIHVHAPAAGSD
                     RLTEAA"
BASE COUNT          316 a          565 c          645 g          275 t
ORIGIN      
        1 aacggacgaa tagtaccacg tcaccatcgg gcctgtcccg agggcccatc tttccgcgtg
       61 acgagccctc acactgatac ggcacactgc gtccgcggca cgactccggg actgccatgg
      121 gccctcggga caagcccgag ggtaacggaa gaaaatatat cacgccgcct cggtcagccg
      181 atcggacccg gcggccggag catggacgtg gatgcgcccg ctctcgcccg cgacggacca
      241 cggacgacgc acgccgccca gctgttccgc gacggcgaga ccggcctgca ggccatcctc
      301 gtggaagccg gaaccgaacc atgcgccggc aaaccagacg ttgcggcggc cctgaagcga
      361 ccagagctcg gactgggcgg cgacggcggc ggcgtcgaag acggggtgtt catagtccca
      421 ttcgccgatg accagttcgg gatccggggt cttggccggg ttcaggctga cgaacagggg
      481 cgggccggga aggctctgaa gctcgttcat ccagtaggtg acgccgccct cgcccgcgcg
      541 gtcggaatag ccgagatggg tccaggcggc ccaggccttg cgccgtttcg gcatcaggga
      601 tgtgtcgcgg tgcaggatga ccttgttagg ccggtattcg atggcgccca gcagacggcg
      661 gtcgtcggcc gagggggctt ccagaagcgc cagcgcctga tcggaatggc tggcgaggac
      721 gacggcgtcg aaccgttcca gacggccgtc gacgaagcgc aaggcggcgc catcgaggcc
      781 gcgcaggacg cctgcgatgc ctgcgttggt gacgatgcgg cccttgaagg cggccgtgag
      841 acgattgacg taggatcggc tgccgccgtc cacggtgcgc caggtcgggc gaaggtccac
      901 cttgagcagg ttgtggttca tgtagaaccg cacgaaggac gccgccggat agctggccat
      961 ctgacccatg gtgcaggacc agatggcggc ggcctgcggc agcagatggg cttcgcggaa
     1021 cagggggccg tagccgccgc gggtcagata cgcctcgagg gtttcgcccg accgctcgag
     1081 ctcggggagg tcgcgcggcg cctgtttctg gaagcgcaac aagtcgccga tcatgcgcca
     1141 gaatttcggg ctggcgtagt tgcgcttctg ggcgaacagg gcaccgaggc cctggctgca
     1201 atattcgaag gcgccgtcat cgatggagac ggcgaacgac atcatggccg gtttcgtcgg
     1261 cacgtcgagg tgttcgaaca gcgcggtcag attgggatag ttgtcctgat tgtagacgat
     1321 gaagcccgtg tcgacggcga ccggctcgct gggcgccggg gcatcgaccg tgtgggagtg
     1381 gccgccgatg cggccgtcgg cctcgaacag ggtgacgtcg tgcgactgcg acagcagcca
     1441 ggcgcaggac aggccggaga tgccggatcc gaccacggcg atgcgcttgc cgccggtctg
     1501 tgaggaggtg gcgacgagat ggggtgacat gccggctccg gtgaaacgtg tccggagcta
     1561 cgagcccaac gctaagacgg atgcccgaca catccaactg cctacgctcc acgtagctgg
     1621 cacggtcatc agcctgtcag gacgcccgca tgagccttgt caccgccgcc atccgccggc
     1681 ttcaggatgc gccgtttccg gacttcgtgg cccggcctgc gatagacagc ctggtcagcg
     1741 aggcgcgcaa gcgtctggat cgggagggcc cgcatgacgc ggccatcttc gcccgcgaca
     1801 t
//
LOCUS       DASVVN010000442         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_463960,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000442 DASVVN010000000
VERSION     DASVVN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463960"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..429
                     /locus_tag="VF686_07075"
     CDS             232..429
                     /locus_tag="VF686_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661612.1"
                     /translation="MADNINNTGSGGGNAGLAFIVGGIVVVLAIVAYFVFARDGAPET
                     KDVNVDVNLPEVSAPAVPSTN"
     gene            433..762
                     /locus_tag="VF686_07080"
     CDS             433..762
                     /locus_tag="VF686_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661613.1"
                     /translation="MTDPNIPPPVEPTPRTEVHHTTINNPPERRGGGSWIGLLVGGLV
                     VVVIIIAFVVFSNGGGINPAKDTNVDIDVDLPRPTLPDAPKMPDLPDVPPVEPPTVPS
                     PSPEPQA"
     gene            complement(766..1041)
                     /locus_tag="VF686_07085"
     CDS             complement(766..1041)
                     /locus_tag="VF686_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661614.1"
                     /translation="MRQGVVLQDFTGPTYVFTKLNAVRPAMIAEATAAARTGAERFAQ
                     DSGAPLGPIRQATQGSFEILARDEIGDESQSPDKKVRVVTTISYRLR"
     gene            complement(1050..1481)
                     /locus_tag="VF686_07090"
     CDS             complement(1050..1481)
                     /locus_tag="VF686_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8661615.1"
                     /translation="MANEKLIPAVVFGGLIAVGLIGSGALIGQGVVNARVGDRSVSVR
                     GLAERNVKADLAILPLRFTASGDVLSDVQSRVDGDLAIVRRFLTAQGYPANAVSLGRL
                     EVADTQSREYANQNGGPRFILAQTVIVRTTDVAASRPRPAP"
     gene            complement(1526..1762)
                     /locus_tag="VF686_07095"
     CDS             complement(1526..1762)
                     /locus_tag="VF686_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8661616.1"
                     /translation="MLKKRSVSLSGHATSVALEPAFWAVLDQVAVAAGLSQAGLMKRI
                     DERRGRTPLASACRLLALAWAGGDRSFAEQESAD"
     gene            1859..2614
                     /locus_tag="VF686_07100"
     CDS             1859..2614
                     /locus_tag="VF686_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEX8661617.1"
                     /translation="MTATADFQTSSRFRPQKLEPITALRAFRRLIRDKEDTAQVFEIM
                     RALSGGSIARGYNRMLGTMEGGRQAFLREELAHKLDDPVWLSKFPSGSVGGAYRAFRE
                     ARGFTAEGLADEARKVAPLVDAPHPVIWYSRRLRDIHDIWHVLTGYGTDALGEACVVS
                     FSYGQTRNLGFAFIGWGAAREIQREVSSVPARRAVWQAYRNGKAAQWLPGLDYEALFE
                     QPLDQVRERLNIRPATVYETVPAGVLAGLKLRA"
     gene            2705..>2957
                     /locus_tag="VF686_07105"
     CDS             2705..>2957
                     /locus_tag="VF686_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661618.1"
                     /translation="MSSPTETAPVARPPLSRTTLLGIGGVIVCAMIWGTTWYAITLQL
                     GTVAPLASIVWRFGLASALLFLGCLIFRLNLRLTWPQHLA"
BASE COUNT          465 a         1037 c          992 g          463 t
ORIGIN      
        1 ctgttcgggg ttcaggccgg ccagatagtc ggcaggcgcc gcctccacgc cattgggacc
       61 gcgcgagcgg gcgaggtcgg agatacgcgg ggcaggggct tcaggcagac tcatcggcgg
      121 aacataaggg gcacgaagcc atctgtcagc gtcggcgtgc gccggttctc gcggaaccgc
      181 gaagcgtgac cgtcgtttcg gctgcatcag caatcaggga agggatcttc catggccgac
      241 aatatcaaca acaccggcag cggcggcggc aatgcgggtc tcgccttcat cgtcggcggg
      301 atcgtcgttg tcctcgccat cgtcgcctat ttcgtcttcg cccgcgacgg tgcgcccgag
      361 acgaaggacg taaacgtcga cgtcaatctg ccggaagtct cggcccccgc cgttccgagc
      421 acgaactgag ccatgaccga ccccaacatc ccgccgcccg tcgaaccgac gccgcggacc
      481 gaggttcatc acaccaccat caacaacccg cccgagcgcc gcggcggcgg tagctggatc
      541 ggcctgctgg tcggcggtct ggtcgttgtg gtgatcatca tcgccttcgt cgtcttctcg
      601 aacggcggcg gcatcaatcc ggccaaggac acgaatgtcg acatcgacgt cgacctgccg
      661 cgtccgaccc tgccggacgc gcccaagatg ccggacctgc ccgacgtacc gccggtcgag
      721 ccgccgaccg tgcccagccc gtcgcccgag ccgcaggcct gaccgtcagc gcaggcggta
      781 gctgatggtg gtcacgaccc ggaccttctt gtccggggac tggctctcgt cgccgatctc
      841 gtcgcgggcg aggatttcga acgagccctg cgtggcctga cggatgggac cgagcggcgc
      901 acccgaatcc tgggcgaacc gctcggcgcc ggttcgagcg gcggccgtgg cctcggcgat
      961 catggccggg cggacggcgt tgagtttggt gaagacgtag gtggggccgg tgaaatcctg
     1021 cagcaccacg ccctgacgca ccaggtcgtt cagggcgcgg gtcgtggcct ggacgcggcg
     1081 acgtccgtgg tgcggacaat gacggtctgg gcgaggatga agcgcgggcc gccgttctga
     1141 ttggcgtatt cacgggattg ggtgtccgcg acctccagcc ggcccagcga gacggcgttg
     1201 gcgggatagc cctgggcagt caggaagcgg cgcacgatgg ccagatcgcc gtccacgcgg
     1261 ctctgaacgt ccgacaggac gtcgcccgac gccgtgaacc gcagcggcag aatggccaga
     1321 tcggccttca cgttgcgctc ggccagtccc cggacgctga cggagcggtc cccgacacgg
     1381 gcgttgacca ccccctgtcc gatcagggcg ccgctgccga tcagtccgac ggcgatcagg
     1441 cctccgaaca cgacagcggg gatcagtttt tcattggcca tgggaacgcc ctccggaccg
     1501 gaggatgcgc caaccttggc cgctgtcaat cggcggactc ctgttcggcg aaagaccggt
     1561 cgccgccggc ccaggccagc gccagcaatc ggcaggccga ggcgaggggc gtgcgtccgc
     1621 gtcgctcgtc gatccgcttc atcagtccgg cctgcgacag gcccgcggcc acggcgacct
     1681 gatccagcac cgcccagaac gccggctcca gcgccaccga ggtggcgtgg ccggacaggg
     1741 atacggagcg tttcttcagc atgcgcgacc ttcgcatacg gccgcgccgg atgcgacgtg
     1801 aacgacgttc acctgctgga aggatcgcgg tatgatcatg ccagcgacag gatccgccat
     1861 gaccgccacc gccgacttcc agacatccag ccgcttccgg ccgcagaagc tggagccgat
     1921 cacggccctg cgggccttcc gccgcctcat ccgcgacaag gaggacacgg cccaggtctt
     1981 cgagatcatg cgcgccctgt cgggcggctc gatcgcccga ggctacaacc ggatgctcgg
     2041 caccatggag ggcggccggc aggccttcct gcgcgaggaa ctggcgcaca agctcgatga
     2101 tcccgtctgg ctgtcgaaat ttccgtccgg ttcggtcggc ggggcctacc gggccttccg
     2161 cgaggcgcgc ggcttcaccg ccgagggtct ggccgacgaa gcccgcaagg tcgccccgct
     2221 ggtcgacgcg ccccacccgg tcatctggta ctcgcgccgc ctgagggaca ttcacgacat
     2281 ctggcacgtc ctgaccggct acggcaccga cgccctgggc gaggcatgcg tcgtctcatt
     2341 ctcgtatggc cagacccgca atctcggttt cgccttcatc ggctggggcg cggcgcgcga
     2401 gatccagcgc gaggtgagca gtgtgccggc gcgccgggcc gtctggcagg cgtaccgcaa
     2461 cggcaaggcc gcccagtggt tacccgggct ggactacgaa gccttgttcg aacagccgct
     2521 ggatcaggtc cgcgaacgcc tgaacatccg ccccgcgacg gtctatgaga cagtccccgc
     2581 cggggtgctg gcggggctga agctgcgggc ctgaacggcg agatccggac gccataagcc
     2641 gcccttatgg cgagcggcca taacccggct tgcgccccgc tccgacgcgg ttcatctgcg
     2701 aaccatgtcc tccccgactg aaaccgcccc cgtcgcccgg ccgcccctgt cacgcaccac
     2761 gcttctgggc attggcggcg tgatcgtctg cgccatgatc tggggcacga cctggtacgc
     2821 catcaccctg cagctgggca cggtggcgcc gctggcctcg atcgtgtggc ggttcggcct
     2881 ggcctcggcc ctgctgttcc tcggctgcct gatcttccgt ctgaacctgc gcctgacatg
     2941 gccgcagcat ctggccg
//
LOCUS       DASVVN010000443         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_227533,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000443 DASVVN010000000
VERSION     DASVVN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227533"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VF686_07110"
     CDS             <1..1293
                     /locus_tag="VF686_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HEX8661619.1"
                     /translation="HSDADGRWRGPDQQVHQADFTVHSTFSLWDTFRAEHPLLTLVAP
                     PARNTDIVNSLIAAQQVSPYGILPVWSFHGQETWTMIGYHAVPVIADAWLKGVPGIDG
                     AAALNAMTASATYRPYGGLGDYMDLGYVPIDREPEAASKTVEYAYDDWTIARMAERLG
                     RADVAAQFDRRAGNWRNTFERETGFVRARLADGSYRTPFDPTAINYGSDYTEGNAWQY
                     SWFAPQDLGGLVNLMGGDAATVAKLDAMFDFDNTGLDYSHAEDIAGLIGQYIHGNEPS
                     HHTGYMYVYAGAPWRTQARLTQIVNSQYHARPDGLAGNDDVGQMSAWLMFTAMGFYPV
                     TPGSNQYVIGRPFTDRTVLNLANGKDFAIVTEGLADDHPYIQSVTLNGQPLARTWIGH
                     EEIMAGGELRFVMGAEPNTTWGVGAEARPYSMTGYLAN"
     gene            complement(1563..>1967)
                     /locus_tag="VF686_07115"
     CDS             complement(1563..>1967)
                     /locus_tag="VF686_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEX8661620.1"
                     /translation="DFLASYTAMQERYRIIPESIEVTTGTARGKRTGLRPEFADACLN
                     LWLGDRDERYRALTAIHYRAMKETSKAAYGYTSLADITTRPMTQGDNCPGYWWSEQMK
                     YYFLMFAEPRRVDLGQLVLSTEANVLRGFVRG"
BASE COUNT          333 a          698 c          616 g          320 t
ORIGIN      
        1 cactccgacg ccgacggccg ctggcgaggc ccggaccagc aggtccatca ggccgacttc
       61 accgtccatt cgacgttttc gctgtgggac accttccgcg ccgagcatcc gctgctgacc
      121 ctggtcgcgc cgcccgcgcg caacaccgac atcgtcaact cgctgatcgc cgcccagcag
      181 gtcagcccct acggcatcct gcccgtctgg tcgttccatg gtcaggagac ctggaccatg
      241 atcggctatc acgccgtccc ggtgatcgca gacgcctggc tcaagggcgt tcccggcatc
      301 gacggcgccg cggccctgaa cgccatgacg gccagcgcta cctaccggcc ctatgggggc
      361 ctgggcgact atatggacct cggttacgtc cccatcgacc gagagcccga ggccgcgtcc
      421 aagacggtcg aatacgccta tgacgactgg accatcgccc gcatggccga acggctgggc
      481 cgggccgacg tcgccgccca gttcgaccgc cgcgcgggca actggcgcaa caccttcgag
      541 cgcgagaccg gcttcgtccg cgctcgtctg gccgacggtt cgtaccggac gcccttcgac
      601 cccaccgcca tcaactacgg atcggactac accgagggca atgcctggca gtattcgtgg
      661 tttgcgcctc aggacctcgg cggtctcgtc aatctgatgg gcggcgacgc ggcgacggtg
      721 gccaagctgg acgccatgtt cgacttcgac aacacgggcc tcgactactc ccacgccgag
      781 gacatcgccg gactgatcgg ccagtacatc cacggcaacg agcccagcca ccacaccggc
      841 tatatgtacg tctacgccgg cgccccgtgg cggacgcagg cgcggctgac ccagatcgtg
      901 aacagccagt accacgcccg ccccgacggc ctcgccggca acgacgacgt cggccagatg
      961 tcggcctggc tgatgttcac agccatgggc ttctatccgg tcacgccggg ctcgaaccag
     1021 tacgtcatcg ggaggccgtt cacggaccgg acggtgttga acctcgccaa cggcaaggac
     1081 ttcgccatcg tcaccgaggg cctcgccgac gaccacccct acatccagtc ggtgaccctg
     1141 aacggccagc cgctggcccg gacatggatc gggcatgagg agatcatggc cggcggagag
     1201 ctgcgcttcg tcatgggagc ggagccgaac acgacctggg gcgtgggggc ggaggcgagg
     1261 ccgtattcga tgacggggta tcttgcgaac tagacgcttg tcatcccgga cgccgcaagg
     1321 cggtgatccg ggacgctcgc gcgctggggc catcaccccg gaagcggcgt cagccgctat
     1381 ccgggggaca gatcagaaca tgatgcggcg gtcacccgtc tcccggacaa ccgctccgcg
     1441 atttccggga ggttggtagc cgggtgcagg tccgtcccgg atcgccgcgt tgcggcgtcc
     1501 gggatgacaa agtgcacgcg gtctgtcctc cccatccgcc ttcgcgaatg gggaggtgaa
     1561 tgtcatcccc tcacaaaccc cctgagcaca ttcgcctccg tgctcagcac cagctggccc
     1621 aggtccaccc gcctcggctc ggcgaacatc aggaagtagt atttcatctg ttccgaccac
     1681 cagtagccgg ggcagttgtc gccctgggtc atcgggcggg tcgtgatgtc ggccagcgag
     1741 gtatagccgt aggccgcctt cgaggtttcc ttcatcgccc gatagtggat cgccgtcagc
     1801 gcccgatacc gctcgtccct atcgcccagc cacaggttca ggcaggcgtc ggcgaattcg
     1861 gggcgcaggc cggtgcgctt gccgcgcgcc gtccccgtcg tcacctcgat cgattccggg
     1921 atgatcctgt aacgctcctg catggccgta tagctggcca gaaagtc
//
LOCUS       DASVVN010000444         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_42495, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000444 DASVVN010000000
VERSION     DASVVN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42495"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VF686_07120"
     CDS             complement(<1..421)
                     /locus_tag="VF686_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661621.1"
                     /translation="MKQIAALAAAALAATLALAACATAPAPASPQDAFFTNLKALCGQ
                     RFEGRVVTTDAADADFARSRLLMHVRDCSTDEVRIPFWVGENRSRTWVVTRTASGVRL
                     KHDHRHEDGTEDELSQYGGDTTDAGTATRQEFPVDQFS"
     gene            467..874
                     /locus_tag="VF686_07125"
     CDS             467..874
                     /locus_tag="VF686_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HEX8661622.1"
                     /translation="MTAAVTLRLTIDDPVPGVRYSLQKDDAPFEPVTAGDDPISFEVP
                     ITLHPDGRMTGPFVRREGPQRRFVYIRIGTSAGEHASVWSRRAKIDIHDIPPALLTPS
                     AVLEVHLPGRGKDGSPACATVRKLGAWKAASHR"
     gene            948..1115
                     /gene="rpmG"
                     /locus_tag="VF686_07130"
     CDS             948..1115
                     /gene="rpmG"
                     /locus_tag="VF686_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX8661623.1"
                     /translation="MAKPASIKIRLNSTADTGFFYVTKKNARTMTEKMVVKKYDPVVR
                     KHVEFKEGKIK"
     gene            1192..1377
                     /locus_tag="VF686_07135"
     CDS             1192..1377
                     /locus_tag="VF686_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661624.1"
                     /translation="MSDPTDEQPAPETPDESPVQRALRLKKAAIEAREKPPRGGRFQR
                     EQAARIATGKSKPWMSR"
     gene            complement(1404..>1797)
                     /locus_tag="VF686_07140"
     CDS             complement(1404..>1797)
                     /locus_tag="VF686_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661625.1"
                     /translation="APLVRGLLPDGGDGLNRFEKWQAVMELPERSDWIESRLNELPLL
                     VRFTDAADPASAAWVNPEGRGNPVTLVRAATRLTDAPLTRGALTEALPWLADLGVSGR
                     PFSVVPGREMRSVSELLNQYDLRLKPRG"
BASE COUNT          319 a          640 c          561 g          277 t
ORIGIN      
        1 tggagaattg gtcgaccggg aactcctgcc gcgtcgccgt gccggcgtcc gtcgtgtcgc
       61 cgccgtactg gctcagctca tcctcggtcc cgtcctcatg ccggtgatcg tgcttcagcc
      121 gcacgccgga ggctgtccgg gtgacgaccc aggtccgcga ccgattctcg ccgacccaaa
      181 agggtatccg cacctcatcc gtcgaacagt cccgcacatg catcagcagc cgggaccggg
      241 cgaaatcggc gtcggcggcg tccgtggtca ccacgcgccc ctcgaaccgc tggccgcaca
      301 gggccttcag attggtgaag aaggcgtcct gcggtgacgc cggagccggc gcggtggcgc
      361 aggcggccaa ggcgagagtg gcggcgaggg cggcggcggc gagggcggcg atctgtttca
      421 tggtatcagg ttggcacgac gggcccgtct cgcctaacct cccgacatga ccgctgccgt
      481 gacgcttcgc ctcaccatcg acgatccggt ccccggggtg cgctacagtc tccagaagga
      541 cgacgcaccg ttcgagccgg tcaccgccgg cgacgatccg atctctttcg aggtcccgat
      601 caccctgcac cccgacggcc gcatgaccgg gcccttcgtg cgccgcgagg gtccgcagcg
      661 ccgcttcgtc tacatccgca tcggcacgtc ggcgggcgaa catgccagcg tctggagccg
      721 ccgcgccaag atcgacattc atgacatccc gcccgccctg ctgaccccca gcgcggttct
      781 cgaggtccat ctgcccggcc gcggcaagga cggttcgccc gcctgcgcca ccgtccggaa
      841 gctcggcgcg tggaaggccg caagtcaccg ttgacttccg gccgcgcctg agtatgttcc
      901 gcgcctctta tttccagcgg ctttcgccgt cgcaaaggta attcccgatg gccaagcccg
      961 cctctatcaa gatccgcctg aactccacgg ccgacaccgg cttcttctac gtcaccaaga
     1021 agaacgcccg gaccatgacc gagaaaatgg tcgtcaagaa gtacgacccc gtcgtgcgca
     1081 agcacgtcga gttcaaggaa ggcaagatca agtaagggct acgccctgct gaatcgcaga
     1141 acgcccggtg gaaacgccgg gcgtttttct ttgccgtgct aggatgattg aatgagtgac
     1201 ccgaccgacg aacagcccgc tcccgaaacg ccggacgaga gccccgtcca gcgtgcgctt
     1261 cggctgaaga aggcggcgat cgaggcgcgc gaaaaaccgc cccgcggcgg ccgtttccag
     1321 cgcgaacagg ccgcgcgcat cgccaccggc aagtccaagc cctggatgag ccgctagacc
     1381 cgggcccgcg gcggatgcct gcatcagccg cgcggcttca ggcggagatc gtactggttg
     1441 aggagttccg acaccgaacg catctccctg ccgggcacga ccgagaacgg tctcccgctg
     1501 acgccgagat cagccagcca cggaagcgcc tccgtcaacg ctcctcgcgt aaggggggcg
     1561 tccgtcagac gcgtcgccgc ccggacaagc gtgaccggat tccctcgtcc ttctgggttc
     1621 acccaggccg ccgacgcagg atcggccgca tcggtgaacc tgacaagcag cggaagctca
     1681 ttgagcctgg actcgatcca atccgatcgc tcggggagtt ccatcacggc ctgccacttt
     1741 tcaaaccggt tgaggccgtc gccgccatcc ggaagaagcc cacgaacgag cggcgca
//
LOCUS       DASVVN010000445         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_464055,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000445 DASVVN010000000
VERSION     DASVVN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464055"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1838)
                     /locus_tag="VF686_07145"
     CDS             complement(<1..>1838)
                     /locus_tag="VF686_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8661626.1"
                     /translation="VVASLLGGWLIAGPTAVGVGVSTPLGVVSTTNFIVVGLFIAIVA
                     ASLRKTLARLDRSVAELSAADARVGESDARFRLMADTAPSPVWMTNANAEVEFVNAAL
                     VKYYGRPADELLGHVWRQTIHPDDQAAVAAAMDAARPQHLPYGFEARFMHADGGWRWM
                     RISVNPRFGTDGTFLGYVGMAFDVSETREALDALAALERRQTFLLELADRLRDLASPE
                     EIMLEAERAVGAELDADRVGYGEVDEERGVVFMSRDWTAGVVSAHGEFRREDLGADLI
                     SDLAEGRLIRIPDVQKDPRTRDALEVFQRLETRALVRAPVIRGGRLRAFMYAHDADVR
                     EWSEADSSLLQEVAVRAWTEIERARAEAAVRESEARFRAIADTAPVLIWVTQQDRTRA
                     FVNQAYVAFNGGTYEDARLADWRAVVHPDDHERIIEESLAGEATGQPFSMEARYLRHD
                     GEYRWLKSFSRPRLGAGGAVIGFVGVAFDVTDIREASDRLMESETRFRTVADSAPALI
                     WMLDAEGRPSFINRRYRTYFGIESELDVVEGWRKLVHPDDIQAFMTGFEEAVERYDRF
                     EGVIRTRHPVMGERWLRCEALPRFDAAGKFQGYVGANVDITEAKRA"
BASE COUNT          291 a          637 c          632 g          278 t
ORIGIN      
        1 tcggcgcgct tggcctcggt gatgtcgaca ttggcgccga catagccctg gaatttcccg
       61 gccgcgtcga agcggggcag ggcctcgcag cgcagccagc gctcgcccat gacggggtgc
      121 cgcgtacgga tgacgccctc gaaacggtcg taccgctcca ccgcctcctc gaagccggtc
      181 atgaaggcct ggatgtcgtc ggggtggacc agcttgcgcc atccctcgac cacgtccagt
      241 tcggactcga tgccgaaata ggtgcggtag cggcgattga tgaagctggg cctgccctcg
      301 gcgtccagca tccagatcag ggcgggcgcg ctgtcggcca cggtgcggaa ccgggtttcg
      361 gattccatca accggtcgct ggcttcccgg atgtccgtca catcgaaggc cacgcccacg
      421 aagccgatga ccgcgccgcc ggcgccgagc cggggccgcg aaaacgattt gagccagcga
      481 tactcgccgt catggcgcag ataccgcgcc tccatcgaga acggctggcc cgtggcctcg
      541 cccgccagcg attcctcgat gatgcgctca tggtcgtcgg gatggaccac cgcgcgccag
      601 tctgcgaggc gcgcgtcttc ataggttccg ccgttgaagg cgacataggc ctgattgacg
      661 aaggcccggg tgcggtcctg ctgcgtcacc cagatcagca ccggcgcggt gtcggcgatg
      721 gcgcggaagc gggcttcgct ttcgcgcacc gcggcctcgg cgcgcgcgcg ttcgatttcg
      781 gtccaggccc ggaccgcgac ctcctgcaac aggctggagt cggcttcgct ccattccctc
      841 acgtcggcgt cgtgggcgta catgaaggcc cgcaaacggc cgccccgaat gaccggcgcg
      901 cgcaccagag cccgggtttc cagccgctgg aagacctcca gcgcgtctcg ggtgcgcggg
      961 tccttctgga cgtcggggat gcggatcaac cgtccttcgg cgagatcgga aatcaggtcc
     1021 gcgccgagat cttcgcgccg gaactcgccg tgcgcgctca cgacgccggc ggtccagtcc
     1081 cggctcatga agaccacgcc gcgttcctcg tccacctcgc cataacccac acggtcggcg
     1141 tccagctcgg cgccgacggc gcgctcggcc tcgagcatga tctcctcggg cgaagcgagg
     1201 tcgcggaggc ggtcggccag ttccagcagg aaggtctgac gccgctccag cgcggcgagg
     1261 gcgtccagcg cctcgcgggt ttcgctgacg tcgaaggcca tgccgacata gccgaggaag
     1321 gtcccgtcgg tcccgaaccg cgggttgacg gagatgcgca tccagcgcca gccgccgtcg
     1381 gcgtgcatga accgcgcctc gaagccgtac ggcagatgct gcggccgcgc ggcgtccatc
     1441 gccgctgcaa ccgccgcctg atcgtccgga tgaatggtct gtctccagac gtggcccaga
     1501 agctcgtccg cgggccgacc gtagtatttg accagggccg cgttgacgaa ttcgacctcg
     1561 gcgttggcgt tggtcatcca caccggcgag ggggcggtgt cggccatcag gcggaaccgg
     1621 gcgtcgctct cgccgacccg ggcgtcggcg gcggagagct cggcgaccga acggtcaagc
     1681 cgggccaggg tctttcgcaa cgaggcggcg acgatcgcga tgaacaggcc gacgacaata
     1741 aagttggtcg tggagaccac cccgagcggc gtggaaaccc cgacgccgac ggctgtgggg
     1801 cccgcaatga gccagccccc gagcagcgag gcgacgac
//
LOCUS       DASVVN010000446         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_362911,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000446 DASVVN010000000
VERSION     DASVVN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362911"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VF686_07150"
     CDS             <1..1866
                     /locus_tag="VF686_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEX8661627.1"
                     /translation="RDDVPELAGMKQALAAFAVDIPAFSLTAGPSGARVALTRPATLR
                     PANGGVLTLRPAAEPIFSAGRGQLGGGALSLTATRGRGQPEAAFAIPDWRLTPTGFTA
                     TLDGRAALDFGLAKGITVQTRGQLTSSNGRVTYVAAGCAPVAVERLELDESDVTELAG
                     QLCPAARPLASIADGGWRSDGTLRGFSAHAPFLAVHFGEIQGGFTAVGGPRGITLDAR
                     VAEATVSDATTPLRFNPLGASGLARMTGEDWTGVFDLTRNGAALGRLTLAHDGAAGAG
                     GVVIDAPSIVFAEGGLQPDDLSPLAGDFVGSPATGSVSFNGRIDWRAGTEGTSSGRLT
                     IPGLDFVSPAGPVKGLRGTIDFTNLAPLTAAPGQRLTVDRLESFTPLTDIQLTFGLDK
                     AAVTVQGGDLAVAGGRVSIEPFAVPLDPTQPITGVIVLDKVQLGQVIAGSGFGDKVLL
                     DALVSGRLPFTSTREGGVRITGGSLAAVQPGRLSIAREALTGLEAGGGGEGVPPNTIQ
                     DLAYQAMENLSFDLLSAEVNSLDEGRIGVLFRIRGRHDPPQKQELRISIADFISREFL
                     NRELPLPSGTGIDLTLDTTLNLNQLVGDLLELNRARSGQPSPLLTPAPTPAPATP"
     gene            1950..2138
                     /locus_tag="VF686_07155"
     CDS             1950..2138
                     /locus_tag="VF686_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEX8661628.1"
                     /translation="MTRTRKRLALLGLMAALGASACTPTVRLQVDPIQIYAKLDADVR
                     VRLDKELQDLLVENPNLF"
     gene            2156..2518
                     /locus_tag="VF686_07160"
     CDS             2156..2518
                     /locus_tag="VF686_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX8661629.1"
                     /translation="MSFRKFFVVAAAIAALGVAAGAAFAQTSAQKSMIDAAKSQGTVG
                     EQADGYLGFRVPASDAALTQAVQATNSARREAYARSAQAAGTTPDVAGARMFEGQLLP
                     RISSGQWYRNTQGQWVQR"
BASE COUNT          382 a         1080 c          822 g          385 t
ORIGIN      
        1 cgcgacgatg tgcccgaact ggccgggatg aaacaggcct tggccgcctt cgccgtcgac
       61 atccccgcct tcagcctgac cgccggcccg tcgggcgccc gcgtagccct gacccggccc
      121 gcgaccctgc gccccgccaa cggcggcgtc ctgaccctga ggcccgccgc cgaaccgatc
      181 ttctccgcag ggcggggcca actcggaggc ggggccctca gcctgaccgc gacgcggggc
      241 aggggacagc ccgaggccgc cttcgccatt cccgactggc ggctgacccc gaccggcttc
      301 accgcgaccc tggatggccg cgccgccctc gacttcggcc tggccaaggg gatcacggtc
      361 cagacccgcg ggcagctgac ctcctcgaac ggccgcgtga cctatgtcgc cgccggctgc
      421 gcccccgtcg cggtcgagcg gctggaactg gacgagagcg acgtcacgga actggccggt
      481 cagctctgtc ccgccgcccg cccattggcc agcattgccg acggcggctg gcgttccgac
      541 ggaaccctgc gcggcttcag cgcccacgcc cccttcctcg ccgtgcattt cggcgagatc
      601 cagggcggct tcaccgccgt cggcggtccc cgcggcatca ccctggacgc ccgcgtggcc
      661 gaggccacgg tctcggacgc caccaccccc ctgcgcttca acccgctggg cgcatcgggc
      721 ttggcccgga tgacgggcga ggactggacc ggcgtcttcg acctgacccg caacggcgcc
      781 gcactcggcc gcctgaccct cgctcacgac ggcgcggcag gcgcgggcgg cgtcgtcatc
      841 gacgcgccgt ccatcgtctt cgccgagggc ggccttcagc cggacgacct cagccccctc
      901 gccggcgatt tcgtcggctc ccccgcgacg ggctcggtca gtttcaacgg ccgcatagac
      961 tggcgcgccg ggaccgaggg gaccagttcc ggccgcctga ccatccccgg cctcgacttt
     1021 gtcagcccgg cgggcccggt gaaaggtctg cgtgggacca tcgacttcac caacctggcc
     1081 cccctgaccg ccgcgcccgg ccagcgcctg acggtcgacc ggctggaatc cttcacgccc
     1141 ctgactgaca tccagctgac cttcggcctc gacaaggcgg cggtgactgt ccagggcggc
     1201 gacctggccg tcgccggcgg ccgcgtcagc atcgaaccct tcgccgtccc gctggacccg
     1261 acccagccta tcaccggcgt tatcgtcctc gacaaggtcc agctgggtca ggtcatcgcc
     1321 ggctcgggct tcggcgacaa ggtcctgctg gacgccctcg tctccggccg tctgcccttc
     1381 acctccaccc gcgagggcgg cgtccgcatc accggcggct cccttgccgc cgtccagccc
     1441 ggccgcctgt ccatcgcccg cgaggccctc accgggctgg aagccggcgg gggcggggag
     1501 ggggtcccgc ccaacacgat ccaggatctc gcctatcagg ccatggaaaa cctgtcgttc
     1561 gacctccttt ccgccgaggt gaacagcctc gacgaaggcc ggatcggcgt cctgttccgc
     1621 atccgcggcc gccacgatcc gccgcagaag caggaactgc gcatctccat cgccgacttc
     1681 atcagccgcg agttcctgaa ccgcgaactg cctctgccgt cgggcacggg catcgacctg
     1741 acgctggaca ccacgctcaa tctcaaccag ctggttggcg accttctgga gctgaaccgc
     1801 gcccgcagcg gccagccctc gcctctcctg acgcccgccc cgacccccgc gcccgccacg
     1861 ccctgatccc gccgccttcg ccgttcagaa cccgttcacc ccgactgccg ttagatcgct
     1921 gcgtattgaa gaccggagac caccgcatca tgacccggac gcgcaaacgc ctcgccctcc
     1981 tcggcctcat ggccgccctc ggcgccagcg cctgcacgcc tacggtccgg ctgcaggtcg
     2041 acccgatcca gatctacgcc aaactcgacg ccgacgtgcg tgtgcgtctc gacaaggagc
     2101 ttcaggacct cctcgtcgaa aaccccaacc tgttctgacg cgaaagttga cccagatgtc
     2161 tttccgcaag ttcttcgtcg tcgccgcggc catcgcggcc ctcggcgtcg ccgcgggcgc
     2221 ggccttcgcc cagacctccg cccagaagtc gatgatcgac gccgcaaagt cgcaaggcac
     2281 ggtcggcgaa caggctgacg gctacctcgg cttccgcgtt cccgcctccg acgcggccct
     2341 gacccaggcg gttcaggcca ccaacagcgc ccgccgcgag gcctacgccc gcagcgccca
     2401 ggccgccggc accacccccg acgtcgccgg cgcccggatg ttcgaaggcc agctcctgcc
     2461 gcgcatcagc tcgggccagt ggtaccgcaa cacccagggc cagtgggtcc agcgctaagc
     2521 gtggctttac ggacccttcg gggtctggcc gggttccctg tgcagccggt tgtgccggcc
     2581 ccgaagggaa ccgatgcgaa ccgcttcgca gtgtatggcc atggccatgc gaatggacgg
     2641 cctcgccacc agcgataccg cacccgaga
//
LOCUS       DASVVN010000447         3675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_438571,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000447 DASVVN010000000
VERSION     DASVVN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438571"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..669)
                     /locus_tag="VF686_07165"
     CDS             complement(82..669)
                     /locus_tag="VF686_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8661630.1"
                     /translation="MPRFESDEDARRLGEALAALRREAGLSQAEAGQRAGMTSQGWGL
                     YESGKRSGLFRPDVQRRLTAAIGATVEALALGRGPADDPPQRAAGTDGMASAGRGFAA
                     SAGPVRRVQLSNDDLAPWAASGVVLEYEPGRWPRRDQGCVIEMADGATRVRLYDRADA
                     DMLVVRGGPGGLAAEERIARADTRSVSAVTARLER"
     gene            complement(726..1280)
                     /locus_tag="VF686_07170"
     CDS             complement(726..1280)
                     /locus_tag="VF686_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX8661631.1"
                     /translation="MFIQTEPTPNPNVLKFLPGRDVAESPTDFVNAEEAAASPLAEAL
                     FALDGVTGVFFGYDYVSVTREAQGPEWTTMKAPILAAVMDHFVSGAPLMRGAVAAAQS
                     SAEDTEIVAEIKQLLDSRIRPAVAQDGGDILFDAFDEETGVLTLRMRGACAGCPSSSA
                     TLKAGVEQMMKHYVPEVTRVEQVL"
     gene            complement(1366..1836)
                     /locus_tag="VF686_07175"
     CDS             complement(1366..1836)
                     /locus_tag="VF686_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8661632.1"
                     /translation="MPRKFLVVVDDSPEFESALRYAARRARSTGGRVAMLRVIPAAAS
                     DAHWAGVREEIERQTRLEAETLLNKWADEAAERSGATPLLLIERGEPQDAIRKVVGED
                     AEIKILVLAAGSGSRGPGPLVAAVVKQGAAFTGRKLPVTIVPGELTENDIEDLA"
     gene            complement(1911..>3675)
                     /locus_tag="VF686_07180"
     CDS             complement(1911..>3675)
                     /locus_tag="VF686_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX8661633.1"
                     /translation="FRLPRSSWQDYDKSKISKGGGVFPRSAKSIELSPEIKAALGIQD
                     DVLDPAALIKAILKAPAELLYFGGIGTYVKAPTESDAQVGDKANDAIRIDGGEVLAKV
                     IGEGANLGLTQAGRIAFARSGGRINTDAIDNSAGVDSSDHEVNIKILTGTAIASGSLK
                     ADKRDALLASMTDEVGLKVLAHNYDQTLAVSLQEAEGPGALDSQQRFMQWLGSKGKLD
                     RKVEGLPDDLKLAEMKTAGQALTRPELAVLTAYSKLELFDDIVASAAPDDAFFKQALV
                     RYFPAPLAKYEADMQRHRLRREIVSTILSNEIVNMAGPTFPERLRQSARCDTGAMVLA
                     FEAARQVFRLDAAWDAVSALDLKIPAEAQTALYREIATVLRRQTFWLARRAMRKGCTV
                     DQLSAAYRPAADALRKAGGAVLSRFEQARLEERVQTFIGLGASEAMARDFAMLRPLVA
                     TVDIGDLAREAKWPEPAMAMLYHQVGAAFDFDRLRAAAGNVPSADHFDRLAVRRLIED
                     LMSEQVLLTRAVAKASKASVGASEAAAEGAVDAWIGSRQATVEGVRASVDEIEASGSG
                     WTFAKLTIANSAIREIASSAS"
BASE COUNT          555 a         1317 c         1229 g          574 t
ORIGIN      
        1 gattctggcc atgtcggttc gtctccttct ccactccgca gagtttcatg gaaacgaaaa
       61 cgttgcaatg aaatagtttc atcagcgttc cagccgcgcc gtcacggcgg acaccgaccg
      121 ggtgtcggcg cgcgcgatcc gctcctcggc ggccaacccg ccgggcccgc cgcgcacgac
      181 cagcatgtcc gcatcggcgc ggtcgtagag ccgcacccgc gtcgcgccgt cggccatctc
      241 gatgacgcag ccctgatccc gccgcggcca gcggccgggc tcatattcca gcaccacgcc
      301 cgaggcggcc cagggcgcca gatcgtcgtt ggacagctgc acccgccgca ccgggccggc
      361 cgacgcggcg aagccccgac ccgcgctggc catgccgtcc gtgccggcgg cgcgctgcgg
      421 cgggtcgtcg gccggccctc gacccagggc cagagcctcg accgtggcgc ctatcgcggc
      481 cgtcagccgc cgctgcacgt cagggcggaa caggccggag cgcttgccgg actcgtacag
      541 gccccagccc tgactggtca tgccggcgcg ctgtccggcc tcggcctggc tcaggccggc
      601 ctcgcggcgc agggccgcca aggcctcgcc gagacgccgg gcgtcctcat cgctttcaaa
      661 ccggggcatg gagagatgat ggcgccgtcc gcgcgcggaa gcgaggacgg attcgtttca
      721 gcgcctcaca gcacctgttc gacgcgggtc acttcgggga cgtagtgttt catcatctgc
      781 tcaaccccgg ccttcagcgt cgcggatgac gacggacagc cggcgcaggc cccgcgcatg
      841 cgcagggtca ggaccccggt ctcctcgtcg aaggcgtcga acaggatgtc gcccccgtcc
      901 tgggccacgg cggggcggat gcggctatcc agcagctgtt tgatctcggc cacgatctcg
      961 gtgtcctcgg cggaggactg cgcagccgcg acggccccgc gcataagcgg cgcgccggag
     1021 acgaaatggt ccatgacggc ggccagtatg ggggccttca tcgtcgtcca ttcggggccc
     1081 tgggcctcgc gcgtcaccga gacatagtcg tagccgaaga agacgccggt cacgccgtcc
     1141 agcgcgaaca gcgcctcggc cagcggcgag gcggccgcct cttccgcatt gacgaaatcg
     1201 gtcggggact ccgccacgtc ccgccccggc aggaatttca ggacgttcgg attgggggtc
     1261 ggttcggtct ggatgaacat ggcgggtaag tgcgccgtga gaagctctgg ttcaaggcct
     1321 acgcttctcc ctccccttca tgcggaggga gaggcgcgtt cggcgctatg ccaaatcctc
     1381 gatatcattc tcggtcagtt cgccgggcac gatcgtcacc ggcagcttgc ggccggtgaa
     1441 ggcggcgccc tgtttgacta ccgcggcgac cagcggaccg gggccgcggg acccgctgcc
     1501 ggcggcgagg accagaatct tgatctcggc gtcctcgccc acgaccttgc ggatggcgtc
     1561 ctggggctcg ccgcgctcga tcaggagcag cggcgtcgcg ccagagcgtt ccgccgcctc
     1621 gtcggcccat ttgttcagca gggtctcggc ctcgagccgc gtctggcgct cgatctcctc
     1681 gcgcaccccc gcccagtggg cgtcggacgc cgccgccggg atgacccgca acatggccac
     1741 ccgcccgccg gtcgaccgcg cccgccgcgc cgcatagcgc agggcggact cgaactccgg
     1801 gctgtcgtcg acgaccacca gaaacttgcg cggcatcagc cctctcccgc accaccgacc
     1861 gcgctcaagc agcgagcgac cgcgtcgcga gcgctagcgc tcaaacaata tcagcttgct
     1921 gacgaagcta tctcacgaat ggcgctgttg gcgatggtca gtttggcgaa ggtccagccc
     1981 gagcccgagg cctcgatctc gtcgaccgag gcgcgcacgc cctcgaccgt cgcctgacgc
     2041 gacccgatcc aggcgtcgac cgcgccctcg gcggccgcct cgctggctcc gaccgaggcc
     2101 ttggaggcct tggccacggc ccgggtcagc agcacctgct cggacatcag atcctcgatc
     2161 agacgccgca ccgccagccg gtcgaaatga tccgccgacg gcacattgcc ggccgcggcg
     2221 cgcagccggt cgaagtcgaa ggccgcgccg acctgatggt acagcatggc catggctggt
     2281 tcgggccatt tcgcctcgcg ggccagatcg ccgatatcga cggtggccac cagcggcctc
     2341 agcatggcga agtcccgcgc catggcttcg gatgcgccca ggccaatgaa ggtctgcacc
     2401 cgctcctcca gccgcgcctg ttcgaaccgc gacagcaccg cgccgccggc cttgcgcagg
     2461 gcgtcggccg ccggacgata ggccgcgctc agctgatcga ccgtgcagcc cttgcgcatg
     2521 gcccggcggg ccagccagaa ggtctggcgg cgcagcacgg tggcgatttc gcgatagagc
     2581 gcggtctgcg cctcggccgg gattttcagg tccagcgccg acaccgcgtc ccaggccgcg
     2641 tcgagacgga agacctgacg cgccgcctcg aacgcgagga ccatggcccc cgtgtcgcac
     2701 cgcgccgact ggcgcagccg ctcggggaag gtcgggccgg ccatgttgac gatctcgttc
     2761 gacaatatgg tcgagacgat ctcgcgccgc agccggtgcc gctgcatgtc ggcctcgtat
     2821 ttcgcgagcg gcgcggggaa ataccggacc agcgcctgtt tgaagaaggc gtcgtcgggc
     2881 gccgccgagg cgacgatgtc gtcgaacagc tccagcttgg aataggcggt cagcacggcc
     2941 agttcgggcc gggtcagcgc ctgaccggcc gtcttcatct cggccagctt caggtcgtcg
     3001 ggcaggccct cgaccttgcg gtccagctta cccttggagc ccagccactg catgaagcgc
     3061 tgctgggaat ccagggcgcc gggcccctcg gcctcctgca aggagacggc cagggtctga
     3121 tcgtaattat gggccagcac cttcagaccg acctcgtccg tcatcgaagc cagcagtgcg
     3181 tcgcgcttgt ccgccttcag cgagcccgag gcgatggccg taccggtcag gatcttgatg
     3241 ttgacctcat ggtcggacga atccacgccg gcggagttgt cgatggcgtc ggtgttgatg
     3301 cggccgcctg agcgggcgaa ggcgatacgg cccgcctggg tcaggcccag attggcgccc
     3361 tcgccgatga ccttggccaa cacctctccg ccgtcgatcc ggatggcgtc attggccttg
     3421 tcgccgacct gggcgtcgct ctcggtcggc gctttcacat aggtgccgat gccgccgaaa
     3481 tagagcagtt cggcgggcgc cttcaggatg gccttgatca gggcggccgg atccagaacg
     3541 tcgtcctgga tgcccagcgc cgccttgatc tcggggctca gctcgatcga cttggccgag
     3601 cgcgggaaga cgcctccgcc cttcgagatc ttcgacttgt cgtagtcctg ccacgacgag
     3661 cgcggcagcc ggaac
//
LOCUS       DASVVN010000448         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_17021, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000448 DASVVN010000000
VERSION     DASVVN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17021"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /gene="folE"
                     /locus_tag="VF686_07185"
     CDS             complement(<1..449)
                     /gene="folE"
                     /locus_tag="VF686_07185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX8661634.1"
                     /translation="MTKPTVTQAEAEAAVRTLIGWAGDDPDREGLLDTPARVARSYKE
                     LFGGYDVEPRQYLERTFEEVGGYNQLVVLKDIRFVSFCEHHMLPVVGVAHVGYLPTDR
                     VVGISKLARVVRGYARRLQIQEKMTSEIALAIEEVLRPQGVGVVIEA"
     gene            560..1108
                     /locus_tag="VF686_07190"
     CDS             560..1108
                     /locus_tag="VF686_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661635.1"
                     /translation="MTPHVHVSALCRCGGVVMRLTGKPIITATCYCDSCRKAAAVLGA
                     RSGAPAITGVDGGTDFVLCRKDRASPVSGADRLSEFRLGPDAHTRRVVAECCGAPMFL
                     EFSAGHWLSVYRDRLSPDDRPPIETRTMLKDLGPAASGLDGAVPSSATQSPTFMWRLL
                     TAWVAMGFRVPKVDYVKGALVV"
     gene            1207..1470
                     /gene="fliQ"
                     /locus_tag="VF686_07195"
     CDS             1207..1470
                     /gene="fliQ"
                     /locus_tag="VF686_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX8661636.1"
                     /translation="MNGAEVLDVGRDALWLTIQLCLPVLLVGLVVGVVIGLVQALTQI
                     QEQTLIYAPKIIAIFVALLLFLPLMGALLGGFMREIAARIAGM"
     gene            1482..>1764
                     /locus_tag="VF686_07200"
     CDS             1482..>1764
                     /locus_tag="VF686_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX8661637.1"
                     /translation="MEPYATSDQVWQGALIFARIGAVLMMLPGVGESYVPPRIRLSLA
                     LVVSLALWPVIAGVLPALPASVGGMAGWIIREVVVGLMIGALLRSFTTAL"
BASE COUNT          244 a          605 c          585 g          330 t
ORIGIN      
        1 tcggcctcga tgacaacccc gacgccctgg ggccgcaaca cttcctcgat ggccagggcg
       61 atctcggagg tcatcttctc ctggatctgc agccggcggg catagccgcg cacgacccgc
      121 gccagtttgg agatgccgac gacccggtcg gtcggcagat agccgacgtg ggccacgccc
      181 acgaccggca gcatgtggtg ctcgcagaag ctaacgaacc gaatgtcctt cagtacgacc
      241 agctggttgt agccgccaac ctcctcgaag gtccgctcca gatactggcg gggctccacg
      301 tcatagccgc cgaacagctc cttgtagctg cgcgcgaccc gagcaggcgt gtccagcaga
      361 ccctcgcggt ctggatcgtc cccggcccac cctatcaggg tgcgaacggc ggcttcggct
      421 tcggcttggg tcacggtcgg cttggtcata gggcccttag agccggaagc gccgtcaggt
      481 tccagtcccg ggatgtcgct ccgttcgcct tgacttacag aactggactg tacagtctag
      541 ttttgtaacg gagtttgcga tgacccccca cgtccatgtt tccgccctgt gccgctgcgg
      601 cggggtcgtc atgcggctga caggcaagcc gatcatcacc gccacctgct attgcgacag
      661 ctgccgcaag gcggccgccg tgctcggcgc ccgatcgggc gccccggcca tcaccggtgt
      721 cgacggcggc acggatttcg tcctgtgccg caaggaccgg gcgtcgcctg tcagcggcgc
      781 ggaccgcctt agcgaattcc ggctcgggcc cgacgcccac acccggcgcg tcgtcgccga
      841 atgctgcggc gctccgatgt ttctggagtt cagcgccggc cactggctga gcgtctaccg
      901 cgatcgcctg tcgccggacg accgcccgcc gatcgagacg cggaccatgc tcaaggatct
      961 gggtcccgcc gcgtccggac tggacggggc tgtcccgtcg tcggcaaccc agtcgccgac
     1021 cttcatgtgg cggttgctga cggcctgggt cgccatgggg ttcagggtcc ccaaggtcga
     1081 ctatgtgaag ggcgccttgg tcgtctgaag gtgcaatccg ggccatctcg ctaacgcccc
     1141 gttaaccacg ctccgggcat ttcttgccgg gcacggccgc gcgttctgcg gccatcggag
     1201 cgtggcatga acggcgcgga agttctggat gtgggccgcg acgccctgtg gctgacgatc
     1261 cagctgtgcc tgcccgtgct gctggtcggt ctggtcgtcg gcgtggtcat cggccttgtc
     1321 caggcgctga cgcagattca ggaacagacc ctgatctacg cgcccaagat catcgccatc
     1381 ttcgtcgcgc ttctgctgtt cctgccgctg atgggcgccc tgctgggcgg cttcatgcgc
     1441 gagatcgccg cccgcatcgc cggcatgtga tcgcctgagc ggtggagcca tacgccacat
     1501 cggatcaggt ctggcagggc gcgctgatct tcgcgcggat cggcgcggtc ctgatgatgc
     1561 tgcccggcgt gggcgaaagc tatgtgccgc cgcgcatccg tctgtcactg gcgctggtgg
     1621 tgtcgctggc cctgtggccg gtcatcgccg gcgtgctgcc ggccctgccg gccagcgtcg
     1681 gcggcatggc cggatggatc atccgcgagg tcgtcgtcgg cctgatgatc ggcgccctgc
     1741 tgcgcagctt caccactgcg ctgg
//
LOCUS       DASVVN010000449         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_122719,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000449 DASVVN010000000
VERSION     DASVVN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122719"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VF686_07205"
     CDS             <1..167
                     /locus_tag="VF686_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8661638.1"
                     /translation="GKQDRAGEGLGLAFVRNSVRRLGGAITVESELGKGSTFMLKFPT
                     RLVLADAGEY"
     gene            167..592
                     /locus_tag="VF686_07210"
     CDS             167..592
                     /locus_tag="VF686_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8661639.1"
                     /translation="MSTAVKIVMVEDDLGHAKLIEKNIRRANIANDIEHFADGGSAMD
                     YLFSEEVRANGPMLILLDLNLPDMSGTDILERVKKDERLRRAPVVVLTTTDDKTEIQR
                     CYDLGCNVYITKPVDYESFAGAIRQLGLFLSVMQAPEIE"
     gene            600..1646
                     /locus_tag="VF686_07215"
     CDS             600..1646
                     /locus_tag="VF686_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX8661640.1"
                     /translation="MRAQGEVIRLLYIDDDRGLSRLVEKELGRHGFAVACAPDGDAGL
                     EILADRAADFDVVALDHYMPGREGLDVLPAIIALKNPPPVVYVTGAQEGRIAVAALRA
                     GAADYVIKDVSEDFTALLRNALEEAVSRRRLQRENEQAQEMVRLARDRAEAMLREVNH
                     RVGNSLQLVSSFMSLQLRQMTDEGARSALKESQARIEAVAHVHRRLYTSDDMSSVAMD
                     EYLEGLIDELGKSVGPGDTSPNITLSAGPMRVSTDQAVSLGVIVTELVTNAVKYAYAP
                     GQGGEIRVILKPDADGRALLTVEDDGPGMGDGKARGTGLGGKIITAMASGLRSKVEYD
                     GEHRGVRARLAFDL"
     gene            1670..2113
                     /locus_tag="VF686_07220"
     CDS             1670..2113
                     /locus_tag="VF686_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8661641.1"
                     /translation="MLQFGLRDPALDYASRPTAFGLVFHDSKLACVRVDRGEGSYFDL
                     PGGAVDGDETEEQALVREFVEETGMSVRPFVRIAEASQYFRKSDGAPINNTGGFWIAE
                     QLSLDPAAKVETDHELVWLHPHTAIAQLRHDAHAWAVTTWLRRHA"
     gene            2202..2384
                     /locus_tag="VF686_07225"
     CDS             2202..2384
                     /locus_tag="VF686_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8661642.1"
                     /translation="MPDHDRIEGAAKNMGGEIKEAAGKLTGDEKLKADGRADQAEGKV
                     QNAVGGLKDTIRDHRN"
BASE COUNT          441 a          872 c          812 g          375 t
ORIGIN      
        1 ccggaaagca ggaccgggcg ggagagggcc tcggcctggc ctttgtgcgc aacagcgtgc
       61 gccgtctcgg cggcgccatc accgtcgagt ccgagcttgg aaaaggctcg acatttatgc
      121 tgaaattccc cacacgcctc gtcctcgcgg acgccggaga gtattgatga gcactgccgt
      181 gaagatcgtg atggtcgagg acgacctggg tcacgccaag ctgatcgaaa agaatatccg
      241 gcgcgccaat atcgccaacg atatcgagca cttcgccgac ggcggctcgg ccatggacta
      301 tctgttcagc gaggaggtcc gggcgaacgg accgatgctg atcctcctcg atctcaacct
      361 gccggacatg tcgggaaccg atatcctcga gcgggtcaag aaggacgaac gcctgcgccg
      421 cgcaccggtc gtggtcctga ccaccaccga cgacaagacc gagatccagc gctgctacga
      481 tctgggctgc aacgtctaca tcaccaagcc ggtggactac gaaagcttcg ccggcgctat
      541 ccggcagctg ggcctgttcc tgtctgtcat gcaagcgccg gagatcgagt gagcgcgcag
      601 tgagggccca gggggaggtc atccgcctgc tctacatcga cgatgaccgc ggcctgtcgc
      661 ggctggtcga gaaggagctg ggccgccacg gcttcgccgt cgcctgcgcc ccggacgggg
      721 acgcgggtct ggaaatcctc gccgatcgcg cggccgactt cgacgtcgtc gccctggacc
      781 actacatgcc gggccgcgag ggcctggacg tcctgcccgc catcatcgcg ctgaagaacc
      841 cgccgcccgt cgtctacgtc accggggccc aggagggccg gatcgccgtg gccgccctgc
      901 gcgccggcgc cgccgactat gtcatcaagg acgtgtccga ggacttcacc gccctgctgc
      961 gcaacgcgct cgaggaggcg gtgtcgcgcc gccggctgca gcgcgagaac gagcaggccc
     1021 aggagatggt ccgcctcgcc cgcgaccgcg ccgaagccat gctgcgcgag gtcaaccacc
     1081 gcgtcggcaa ctccctgcag cttgtgtcca gcttcatgtc gctgcagctg cgtcagatga
     1141 ccgatgaagg cgcccggtcc gccctgaagg aatcccaggc ccggatcgag gccgtggccc
     1201 acgtgcaccg ccgcctctac acctcggacg acatgtcgtc ggtggccatg gacgaatatc
     1261 tggaaggctt gatcgacgaa ctgggcaagt ccgtcggccc gggcgatacc tcgccgaaca
     1321 tcaccctcag cgccgggccc atgcgcgtct cgaccgacca ggccgtgtcg ctgggcgtga
     1381 tcgtcaccga actggtcacc aacgccgtca aatacgccta cgcccccggt cagggcggcg
     1441 agatccgggt catcctcaag cccgacgccg acggccgcgc cctgctgacg gtcgaggacg
     1501 acgggcccgg catgggcgac ggcaaggcgc gcggcacggg tctgggtggc aagatcatca
     1561 ccgccatggc ctcgggcctg cgctcgaagg tcgagtacga cggcgagcat cgcggcgttc
     1621 gcgcccgcct cgccttcgat ctttgaggac gcccccgcgt tacccgccca tgctccagtt
     1681 cggcctccgc gatcccgccc tcgactacgc ctcgcgcccc acggcgttcg gcctggtgtt
     1741 ccatgactcg aaactggcct gcgtccgcgt cgatcggggc gagggcagct atttcgatct
     1801 cccgggcggc gccgtcgacg gcgacgagac cgaggaacag gcgctggtcc gcgagttcgt
     1861 cgaggaaacc ggcatgtccg tccggccctt tgttcgcatc gccgaggctt cccagtattt
     1921 ccgcaaatcg gacggcgccc cgatcaacaa caccggcggt ttctggatcg ccgaacagct
     1981 gtcgctggat ccggccgcca aggtcgagac cgatcacgaa ctcgtctggc tgcatcccca
     2041 tacggccatc gcgcagctgc gccacgacgc ccacgcctgg gccgtgacca catggctgag
     2101 gcggcacgcc tagcggagcc atacggaacg gccggcccgc cggaccgctc tccttatgta
     2161 ggaacgtggt catagcggcc acgctgttca ggagagattg catgcccgat catgaccgta
     2221 tcgaaggcgc cgccaagaac atgggcggcg agatcaagga agccgccggc aagctgaccg
     2281 gcgacgagaa gctgaaggcc gatggccggg ccgaccaggc cgagggcaag gtccagaacg
     2341 ccgtcggcgg gctgaaagac accatccgcg accaccgcaa ctaggtcttg tcatcccggg
     2401 cgtcgcgcag cggcgacccg ggacgctttg cccgcgataa ctcggacctc ccggaagctg
     2461 cgtcagcagc gatccgggag acgtgtcagg cgccccctgt
//
LOCUS       DASVVN010000450         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_363077,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000450 DASVVN010000000
VERSION     DASVVN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363077"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF686_07230"
     CDS             complement(<1..450)
                     /locus_tag="VF686_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8661643.1"
                     /translation="MRPVALAALLALAACADAEGVAPANGNATASPAADGRPFRVEVL
                     GRFDQPWAMAFLPNGTLLVSERGGRLRLRTGDGRVLDVAGVPQVDAGGQGGLGDVVLH
                     PDYRHNSYVYLSWVEAGQGDTRGAVVGRARFVRTEDGASLSGLQILYR"
     gene            547..1308
                     /locus_tag="VF686_07235"
     CDS             547..1308
                     /locus_tag="VF686_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8661644.1"
                     /translation="MSGLAGVLLAAKRRIENPVLLGFLLLVGLGYVFIQIASEMAEGD
                     TMSFDRWLIEALRSAADPSIPVGPAWLRAAMVDVTALGGVSVVTLLTVVVVFYLVTVR
                     KEATALFVAASITGGALLGVALKAAFMRPRPDVVAHLVDVTSTSFPSGHATNSAVVYL
                     TLGALLARTQTSRRVRAYLMAVAIALTLTVGFSRVYLGVHWPSDVVAGWCVGAAWATL
                     CALLARTLQRRRTLEQPTADEPDPDTTKQTITQTD"
     gene            complement(1331..>2267)
                     /locus_tag="VF686_07240"
     CDS             complement(1331..>2267)
                     /locus_tag="VF686_07240"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8661645.1"
                     /translation="GAGQFDNISTDGERGLLGLAALPDYQSTGRFVVYVTNAGGDIEI
                     RLCRRDSNGLGAARLCQVLFTIEHSQFNNHNGGWMGFGPDGNLYIATGDGGGGDPLGN
                     AQNTSSLLGKILRITIVDDPSAWAPAAGNPFLGGGGAREVFASGLRNPFRASFSGNAL
                     LIGDVGENAVEEIDVMPVASPGLNFGWNFREGTLPFTGTAPTGLTDPASEYLHGTGPR
                     QGRSVIGGYVYRGAVQALQGRYFFADFVSRNVWTFPAGLILSGQPVPSSRYERRNADL
                     IPNAGALNQPVSFGEDSAGNLYMVDFDGEIFLIGG"
BASE COUNT          344 a          756 c          831 g          336 t
ORIGIN      
        1 gcggtagagg atctggaggc ccgagaggct cgcgccgtcc tcggtgcgga cgaaccgggc
       61 ccggccgacc acggcgccgc gcgtgtcgcc ctggcccgcc tcgacccagc tcagatagac
      121 gtaggagttg tggcggtaat ccggatggag gacgacgtcg ccgaggccgc cctggccgcc
      181 ggcgtcgact tgcgggacgc cggcgacgtc gagcacgcgg ccgtcgccgg tgcgcagcct
      241 gagccggccg ccgcgctcgc tgacgagcag ggtcccgttg ggcaggaaag ccatcgccca
      301 gggctggtcg aaccggccga ggacttcgac gcggaacggg cggccgtccg cggcggggga
      361 agcggtggcg ttgccgttgg ccggggccac cccctctgcg tcggcgcagg cggcgagggc
      421 gagcagggcg gcgagggcga cggggcgcat ggggatcgtc tccggtgacg gcgtggctga
      481 gacgcgccat aagcgcagcg gcgcgtgcgg gcaaagcggc gaatggtccg taaagggagc
      541 gaacgaatga gcggattggc aggcgtcctg ctggcggcga agcggcggat cgagaatccg
      601 gtgctgctcg gattcctgct cctggtcggc ctcggctacg tctttatcca gatcgcctcc
      661 gagatggccg agggcgacac gatgagtttc gaccggtggc tgatcgaggc gctgcgcagc
      721 gcagccgatc cctcgattcc ggtcggtccc gcctggctgc gcgcggcgat ggtcgacgtg
      781 accgcgcttg gcggcgtctc ggtggtgacg ctgctgaccg tcgtcgtggt cttctatctg
      841 gtcaccgtcc gcaaggaggc caccgccctg ttcgtggcag cttcgatcac cggcggggcg
      901 cttctcggcg tcgcgctcaa ggccgccttc atgcggccgc ggccggacgt cgtcgcccat
      961 ctcgtcgacg tcaccagcac cagcttcccg agcggtcacg ccaccaacag cgccgtcgtc
     1021 tatctgacgc tgggggcgct gctggcgcgc acgcagacga gccgccgggt gcgggcctat
     1081 ctgatggcgg tggcgatcgc gctgaccctg accgtcggct tctcgcgcgt ctatctcggc
     1141 gtccactggc cgagcgacgt cgtcgccggc tggtgcgtcg gcgccgcctg ggcgacgctc
     1201 tgcgccctcc tggcccgcac cctccagcgc cgaaggacgc tcgagcagcc gacggcggac
     1261 gaacccgatc ccgacacgac gaaacagacg atcacccaga ccgactagac gggctcccga
     1321 caagcccctc ctaccccccg atgaggaaga tctccccgtc gaaatcgacc atgtagagat
     1381 tgccggccga atcctcgccg aaggagacgg gctggttcag agcgccggca ttcgggatca
     1441 gatcggcgtt gcggcgttcg tatcggctgg acggcacggg ctgtcccgaa aggataaggc
     1501 cggcggggaa ggtccagacg ttgcggctga cgaagtcggc gaagaagtaa cggccctgaa
     1561 gcgcctggac ggcgccccga tagacgtagc cgccgatgac cgaacggccc tgtctgggtc
     1621 ccgtcccgtg gagatattcg gaagcggggt cggtcaggcc ggtgggggcg gttccggtga
     1681 atgggagcgt gccctcgcgg aagttccagc cgaagttcag gccggggctg gcgacgggca
     1741 tgacgtcgat ctcctccacc gcattctccc cgacgtcgcc gatcagcaag gcgttgccgg
     1801 agaaagaggc gcggaagggg ttgcgaaggc cggaggcgaa gacctcgcgc gccccgccgc
     1861 cgccgagaaa cggattgccc gccgcgggcg cccaggcgga cgggtcgtcg acgatcgtga
     1921 tccggaggat cttgccgagc agcgagctgg tgttctgagc attgcccagc gggtcgccgc
     1981 cgccgccgtc gccggtcgcg atatagaggt tgccgtccgg cccgaagccc atccagccgc
     2041 cattgtgatt gttgaactgg ctgtgctcga tggtgaacag cacctggcac aggcgcgccg
     2101 cgcccagccc gttggagtcg cgccggcaca gccggatctc gatgtcgccc ccggcattgg
     2161 tgacgtagac gacgaacctg cccgtcgact ggtaatcggg aagcgccgcg agccccagca
     2221 ggccgcgttc gccgtcggtc gagatgttgt cgaactggcc ggccccg
//
LOCUS       DASVVN010000451         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_69903, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000451 DASVVN010000000
VERSION     DASVVN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69903"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF686_07245"
     CDS             complement(<1..300)
                     /locus_tag="VF686_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX8661646.1"
                     /translation="MENAAYVGLSRQMTLRRELDIVANNIANADTTGFKVEQLLLGSE
                     VGERARNAFIRPGVSFVLDNGVGRDYGQGALGQTGRGLDFAIEGEGAFFKVTDGAG"
     gene            476..1129
                     /locus_tag="VF686_07250"
     CDS             476..1129
                     /locus_tag="VF686_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX8661647.1"
                     /translation="MFKFGKKKGAAAGDDASLPAVSDAPMPEGEEGDAPKKKKLPLLF
                     IIIPAALLVLGGGGGAAFFLLQPKAAAAEDGGHGAEKKGGHAKKEDKKGGGHGGGGDA
                     AADPSLGVIAEGPDGVTFFTLPDMTVNIQSPDGRPTYLKLKLTLETKDAHVAEQLQAE
                     MPRMQDMFQGFLRELRPEDLAGSAGSFQLRAEILRRVNLIAAPGKVDAVLIEEMLVN"
     gene            1132..>1560
                     /locus_tag="VF686_07255"
     CDS             1132..>1560
                     /locus_tag="VF686_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX8661648.1"
                     /translation="MTDAAFADDADPFAETEDPLAEGEAEERPAALAERVLNQDEIDS
                     LLGFDLGDDDGSERSGIRAIINSALVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVE
                     VSLDNISSIRFGDYLNSIPLPAILSVFRAEELDNLGLLTID"
BASE COUNT          279 a          520 c          512 g          249 t
ORIGIN      
        1 gcccgccccg tcggtgacct tgaagaacgc cccttcgccc tcgatggcga agtccaggcc
       61 ccggcccgtc tggcccaggg cgccttggcc gtagtcgcgg ccgacgccgt tatccagcac
      121 gaagctgacg ccggggcgga tgaaggcgtt gcgggcgcgc tcgcccacct cggagccgag
      181 cagcagctgt tcgaccttga agccggtcgt gtccgcattg gcgatgttgt tggccacgat
      241 gtcgagctcg cggcgcagcg tcatctggcg tgacagtccg acataggcgg cgttttccac
      301 gggcggctcc tttcggatca gccctcgcaa cggtcgtgcc aatgaggtta acccagcaat
      361 gacgggcatt tcagccgcac agcttgatcc ggagcccggc aggttttgcc ggttcctaac
      421 gcctcgttaa ccaaaaagcg ttcgtatgga ccgagagatt ctcggggcgc gtagcatgtt
      481 caaattcggc aagaagaagg gtgcggctgc gggcgatgac gccagcctgc ccgccgtgtc
      541 cgacgcaccc atgccggaag gcgaagaggg cgacgcgccg aagaagaaaa agctccccct
      601 gctgttcatc atcatccctg cggcccttct ggtgctgggc ggaggagggg gcgcggcctt
      661 cttcctgctg cagcccaagg ccgcggcggc cgaagacggc gggcacggcg ccgagaagaa
      721 gggcggccac gccaagaagg aggacaagaa gggcggaggg catggcggcg gcggcgacgc
      781 ggcggccgat ccgtcgctcg gcgtgatcgc ggaaggccct gacggcgtca ccttcttcac
      841 actgcccgac atgacggtga acattcagtc gccggacggc cgcccgacct atctcaagct
      901 gaagctgacg ctggaaacca aggacgccca tgtcgccgag cagttgcagg ccgagatgcc
      961 gcggatgcag gacatgttcc agggcttcct gcgcgaactc cgccccgagg acctcgccgg
     1021 gtccgccggc agcttccagc tgcgggccga aatcctgcgc cgcgtcaatc tgatcgccgc
     1081 gccgggcaag gtggacgccg tgctgatcga agaaatgctg gtgaactagg catgaccgac
     1141 gcggcattcg ccgacgacgc cgatccgttc gccgaaacgg aagacccgct ggccgaaggc
     1201 gaggcggaag agcgcccggc cgctctggcc gaacgggtcc tgaaccagga cgagatcgac
     1261 agcctgctgg gcttcgacct cggcgatgac gacggctccg agcgttcggg catccgggcc
     1321 atcatcaact ccgcgctggt gtcctacgag cgcctgccga tgctcgagat cgtcttcgac
     1381 cgcctggtgc ggctgatgac gacgtccctg cgcaacttca cctcggacaa cgtcgaggtc
     1441 agtctcgaca acatctcgtc gatccgcttc ggcgactatc tgaactccat ccccctgccg
     1501 gccatcctgt cggtgttccg cgccgaggaa ctcgacaacc tcggcctgct gaccatcgat
//
LOCUS       DASVVN010000452         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_331959,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000452 DASVVN010000000
VERSION     DASVVN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331959"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VF686_07260"
     CDS             complement(<1..274)
                     /locus_tag="VF686_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX8661649.1"
                     /translation="MTDTPASAPVSGGQSTGKRIELTLRALFLGCILAVIFTAANTYL
                     GLKVGLTFASAIPAAVISMAILRMFKTSTIWENMTVQTVASVGGAMS"
     gene            410..862
                     /locus_tag="VF686_07265"
     CDS             410..862
                     /locus_tag="VF686_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8661650.1"
                     /translation="MRKSITAAVLGLLCFGGAANAQGDGAAGVIQGRQAAMMLSGVAM
                     GSIKASIDAGQAPATQRFNTRALARWAHAVPGMFPAGSGSEAGVPTKAKPDVWSDRAS
                     FEARAADYAAAADRLAELAAGEDAAAFTAQWTVVRQSCQACHTPYKAD"
     gene            complement(868..1665)
                     /locus_tag="VF686_07270"
     CDS             complement(868..1665)
                     /locus_tag="VF686_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8661651.1"
                     /translation="MAGRVEGKKALITGAAGGLGEAMAWMLAREGAKVAVTDIDGPRA
                     QALAAAINADHPGAAFAFTHDVADEVQWISVIEAAVAAMGGLSILVNNAGVGGPLTFV
                     EADTVENWQRQYDINLKSVMLGCKHAMPHLRGAAAKGPGGASIVNISSIAGLAGAPGM
                     GPYNATKAAVWMYTKTIALEAAKQDWNVRCNSVHPVFIKTAILDPFIAMAGGDEAKAH
                     ERLSRGIPLKRIGEPDDVAYCVLYLASDESKFVTGTEFRIDGGMTAQ"
     gene            complement(1665..1964)
                     /locus_tag="VF686_07275"
     CDS             complement(1665..1964)
                     /locus_tag="VF686_07275"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX8661652.1"
                     /translation="MTDRIDVAEALKGLPLWREDAGDRPAIARKLVFADFNAAFGFMT
                     CVALKADKVDHHPEWSNVYNRVEILLTTHDAGGVTEKDVDMARFVDETAAAMGGR"
     gene            2083..2793
                     /locus_tag="VF686_07280"
     CDS             2083..2793
                     /locus_tag="VF686_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8661653.1"
                     /translation="MDNMWIAALDPADRKRIEPHLNERPFAQGQMLYDAGEAVEEVWF
                     PMSGVVSLMTILDGDRMIETAAIGREGLVGVTCGPLNGRAASRAIAQVDGVSACCPSD
                     VFADALSKSEPMRMALAQFTESLFAQVQQAAACNAQHRLDERLARWLLTIHDRADSDR
                     FGLTQADIAGMLGVRRATVSEVGAELEEKGLIRRGRGWIEITDRPALEKASCGCYGAM
                     RGLMKDLGVPRPEPGADA"
     gene            2863..>3018
                     /locus_tag="VF686_07285"
     CDS             2863..>3018
                     /locus_tag="VF686_07285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8661654.1"
                     /translation="MTDTAQPSGKTSSFGFRDVDAKEKVRMVRGVFDSVASNYDLMND
                     LMSAGVHR"
BASE COUNT          466 a         1065 c         1024 g          463 t
ORIGIN      
        1 agctcatcgc tccgccgacc gaggcgacgg tctggacggt catgttctcc cagatggtcg
       61 aggtcttgaa catccgcagg atggccatcg agatgaccgc cgccggaatg gccgaggcga
      121 aggtcagtcc gaccttcagg cccagatagg tgttcgcggc ggtgaagatc accgccagga
      181 tgcagcccag aaacagggcg cgcagcgtca gctcgatccg cttgcctgtg ctttggccgc
      241 cgctgacggg cgcgctcgcg ggtgtgtcgg tcatggggtt cctctccctc gttggccgga
      301 cctaagccgg aaaagcgccg ggcgtcgaga gggcgcggat cagaaccggc ccgcgcattg
      361 actccgctgg cgcggcggcg gagcttcgcc gcaacgggga gagacgacga tgcggaaatc
      421 gatcacggcg gcggtgctgg gcctgttgtg cttcggcggc gcggccaatg cacagggaga
      481 cggcgcggcg ggcgtgattc agggccgtca ggcggcgatg atgctgtcgg gcgtggccat
      541 gggctccatc aaggcgtcga tcgacgccgg gcaggccccc gccacccagc gcttcaacac
      601 ccgcgccctg gcgcgctggg cccatgccgt ccccggcatg ttccccgccg gcagcggttc
      661 ggaggcgggc gttcccacga aagcgaagcc cgacgtctgg agcgatcgcg ccagcttcga
      721 ggcccgggcc gcggactacg ccgccgcggc cgaccgtctg gccgaactgg ccgccggcga
      781 ggacgccgcc gccttcaccg cccagtggac ggtggtccgg cagagctgcc aagcctgcca
      841 caccccctac aaggccgact gaccggccta ttgggctgtc atgccgccgt cgatccggaa
      901 ctcggtcccg gtgacgaatt tcgactcgtc cgaggccagg tagaggacgc agtacgccac
      961 gtcgtcgggc tcgccgatcc gcttcagcgg gatgccgcgc gacagccgtt cgtgcgcctt
     1021 ggcctcgtcg ccgccggcca tggcgatgaa cggatcgagg atcgccgtct tgatgaagac
     1081 cggatgcacc gagttgcaac gcacgttcca gtcctgtttg gcggcctcca gggcgatggt
     1141 cttggtgtac atccacaccg ccgccttggt ggcgttatag ggccccatgc ccggcgcccc
     1201 cgccagtccg gcgatcgacg agatgttgac gatcgaggcc cctcccgggc ctttggcggc
     1261 cgcgccgcgc agatgcggca tggcgtgttt gcagcccagc atgaccgact tgaggttgat
     1321 gtcgtactgg cgctgccagt tctcgacggt gtcggcctcg acgaaggtca gcggcccgcc
     1381 gacgccggcg ttgttgacca gaatggacag tccgcccatc gccgcgaccg cggcctcgat
     1441 caccgagatc cattgcacct cgtccgccac gtcgtgtgtg aaggcgaaag cggcgccggg
     1501 atggtcggcg ttgatggcgg cggccagcgc ctgcgcgcgc ggcccgtcga tgtcggtgac
     1561 ggcgaccttc gccccttcgc gcgccagcat ccaggccatg gcctcgccca gcccgccggc
     1621 ggccccggtg atcagcgcct tcttcccttc gaccctcccg gccatcaccg acctcccatc
     1681 gcggcggccg tctcgtcgac gaaacgcgcc atgtcgacat ccttctccgt cactccgccc
     1741 gcgtcatgcg tcgtcagcag gatttcgacg cggttgtaga cgttcgacca ttccggatgg
     1801 tggtcaacct tgtcggcctt cagcgccacg caggtcatga agccgaaggc ggcgttgaag
     1861 tccgcgaaga caagcttgcg cgcgatcgcg ggccggtcgc cggcgtcctc gcgccacagg
     1921 ggcaggccct tcagggcttc cgcaacgtca atccggtcgg tcatcgcatc ctccgcgtcc
     1981 ccttcgccct atcccgccgc aaggactgtc acaagcgcgc ggaacgcacg gcgaagcttc
     2041 ggcgttaccg agcgtcgcta tccaagggaa caggagcacg ccatggacaa tatgtggatc
     2101 gccgcgctgg atcccgcgga tcggaaacgg atcgaaccgc atctgaacga gcggcccttc
     2161 gcccagggcc agatgctgta cgacgccggc gaggccgtgg aggaggtctg gttccccatg
     2221 agcggcgtcg tctccctgat gaccattctc gacggcgacc gcatgatcga gaccgccgcc
     2281 atcggccgcg agggtctggt cggcgtgacc tgcggcccgc tgaacggccg cgccgccagc
     2341 cgcgccatcg cccaggtcga tggcgtctcg gcctgctgtc cgtccgacgt cttcgccgac
     2401 gccctgtcga agagcgagcc catgcggatg gcgctggccc agtttaccga aagcctgttt
     2461 gcacaggtgc agcaggcggc cgcctgcaac gcccagcacc ggctggacga gcgactggcc
     2521 cgctggctgc tgaccatcca cgaccgcgcc gacagcgacc gtttcggcct gacccaggcc
     2581 gacatcgccg gcatgctggg cgtgcgacgc gccaccgtct ccgaggtcgg cgcggagctg
     2641 gaggaaaaag gcctgatccg ccggggccgc ggctggatcg agatcaccga ccgccccgcc
     2701 ctcgaaaagg cgtcctgcgg ctgctacggc gccatgcgcg gcctgatgaa ggacctcggc
     2761 gtgccgcgtc ccgagccagg cgctgacgcc tgaacgggca gccccgccgc cgcgaccacg
     2821 cggcccggtg acggcggcgc ggcgacgcgc tagaccgcca gcatgaccga cacggcccag
     2881 ccttccggca agacatcctc cttcggcttt cgcgacgtcg acgcgaagga gaaggtgcgg
     2941 atggtgcgcg gcgtgttcga cagcgtggcg tcgaactacg acctgatgaa cgacctgatg
     3001 agcgcgggcg tgcaccgg
//
LOCUS       DASVVN010000453         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_412105,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000453 DASVVN010000000
VERSION     DASVVN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412105"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF686_07290"
     CDS             complement(<1..704)
                     /locus_tag="VF686_07290"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8661655.1"
                     /translation="MLDRRRLLLSSAGLGLAAALPAVAQTPAMSEADARLDAMLTRWF
                     NENVDLSPEYATNLGLDRDGRAALTGKLRQVGLADAQADRERARARWAEIQTVPETGL
                     SDQAKVSLAVYRFTAEGAARAADIPYGGGIGPYVVTQRSGAYFSVPDFMANQHRVETQ
                     ADAEAFLSRLSAFAVVLDQENERIVADAALGVMLPSFLMDKTLTQLRTLRDTPPADMA
                     MVQALVRKTGDKGIAG"
     gene            complement(777..>2457)
                     /locus_tag="VF686_07295"
     CDS             complement(777..>2457)
                     /locus_tag="VF686_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8661656.1"
                     /translation="ESPETLTSLGMDKGPMAAMKSRLDDRSQAKIDADRAVFRGQMAE
                     LKGIDRAALPATEGVYHDTLTFFGDTQMMGDRFPYGGGGFGPAPYTISQLTGSYQNLP
                     DFLDTQHAIETAEDAEAYLSRIAAFPTALDQETTRMQADFAAGAVPPDFIIDKTLLQL
                     ANLYDTPAGQSVMTTSVVRRTAEKGVAGDWGARAQRIVESEVYPALRRQAEAMRARRA
                     DATHDAGVWRLPDGEAYYDWGIRSYTTSTLSGAEIHDLGLQQVAELSARADVLLKAQG
                     LTQGSVGERISALGKDPAQIYPNTDAGKEQLLADLNAQMAEMAKRLPQYFGRIPVAPV
                     EIRRVPTFIEAGAPGGYYNSPALDGSRPGAYYINLRDTAEWPRMQLPTLTYHEASPGH
                     HHQIALQQEQAGRPLLMKVLGFSAYSEGWGLYAEQLADEMGVYADNPIGQIGYLQSLL
                     FRATRLVADSGLHHKRWSREQTIRYMVETLGDSETAVTTEVERYCVWPGQASSYKVGH
                     NKWVELRGKAQAALGDRFDIRAFHDAGMNAGGVPLTVLDKIMSDWTAAQRA"
BASE COUNT          363 a          807 c          875 g          412 t
ORIGIN      
        1 tcgccggcga tccccttgtc gcccgtcttg cgaaccagcg cctgaaccat ggccatgtcg
       61 gccggcgggg tgtcgcgcag ggtgcgcagc tgggtcagcg tcttgtccat caggaacgac
      121 ggcagcatga cgcccaaggc ggcgtcggcg acgatccgct cattctcctg atccagcacc
      181 accgcgaagg ccgacaggcg cgacaggaag gcttcggcgt cagcctgtgt ctcgacccga
      241 tgctggttgg ccatgaagtc gggcaccgag aaataggcgc ccgaccgctg ggtcacgaca
      301 tagggcccga tgcccccgcc ataagggatg tcggccgcgc gcgccgcgcc ctcggccgtg
      361 aaacgataga cggccagcga gaccttggcc tgatccgaca ggcctgtttc gggcacggtc
      421 tggatttcgg cccaccgcgc gcgggcgcgc tcccggtccg cctgcgcgtc cgccagaccg
      481 acctgccgca gcttgcctgt aagggcggcg cgcccatccc ggtccagccc cagattggtg
      541 gcgtactccg gcgacaggtc gacgttctcg ttgaaccagc gggtcagcat ggcgtccagc
      601 cgggcgtccg cttcggacat ggccggtgtc tgggcgacgg cgggcagggc agctgccaga
      661 ccgagacccg cggaggacag cagaaggcgg cggcggtcga gcatggggaa atcctcggat
      721 ggcgatgacg gtcagtccgg gccgccgctg gcgaacccgg gccggtggtt tcaggttcag
      781 gctcgctggg cggcggtcca gtcggacatg atcttgtcca gtaccgtcaa cggcacgccg
      841 ccggcgttca tgcccgcatc gtggaaggcc cggatgtcga aacggtcgcc cagcgcggcc
      901 tgggccttgc cgcgcagctc gacccatttg ttgtggccga ccttgtagct ggatgcctga
      961 cccggccaga cgcaatagcg ctcgacctcg gtggtgaccg cggtttccga gtcgcccagg
     1021 gtttcgacca tgtagcggat ggtctgttcg cggctccagc gcttgtggtg caggcccgag
     1081 tcggcgacca gccgggtggc gcggaacagc agcgactgca gataaccgat ctggccgatc
     1141 ggattgtcgg catagacccc catctcgtcg gccagctgtt cggcgtacag gccccaaccc
     1201 tcggaatagg cggagaagcc gagcaccttc atcagcagcg gtcggccggc ctgttcctgc
     1261 tgcagggcga tctggtggtg atggcccggc gacgcttcgt gataggtcag ggtcggcagc
     1321 tgcatccgcg gccattcggc cgtgtcccgc aggttgatgt agtaggcgcc cgggcgcgaa
     1381 ccatccagcg ccggcgagtt gtagtagccg ccgggcgcgc ccgcctcgat gaaggtcggc
     1441 acgcggcgga tctcgaccgg ggcgacgggg atgcgcccga aatactgcgg caggcgcttg
     1501 gccatctccg ccatctgggc gttgaggtcg gccagcagct gttccttgcc cgcgtccgtg
     1561 ttcgggtaga tctgggccgg gtccttgccg agggcggaga tgcgctcccc caccgatccc
     1621 tgggtcaggc cctgggcctt cagcagcacg tcggcccggg ccgaaagctc agccacctgt
     1681 tgcaggccga gatcgtggat ctctgctccc gacagggtcg aggtggtgta gctgcggatg
     1741 ccccagtcgt aataggcctc gccgtcgggg agccgccaga cgccggcgtc gtgggtggcg
     1801 tcggcgcggc gggcgcgcat ggcctcggcc tgacgacgca gggccgggta gacttcgctc
     1861 tcgacgatcc gctgggcgcg agcgccccag tcgccggcca cccccttctc ggccgtccgt
     1921 cgcacgacgg aggtggtcat caccgactgg ccggcaggtg tgtcatacag gttggcgagc
     1981 tgcagcaggg tcttgtcgat gatgaagtcg ggcggcacgg ccccggccgc gaagtcggcc
     2041 tgcatccgcg tggtttcctg atccagcgcc gtcgggaagg cggcaatccg ggacagatag
     2101 gcctcggcgt cctctgcggt ttcgatcgcg tgctgcgtgt cgaggaagtc gggcaggttc
     2161 tgatacgaac cggtcagttg ggagatcgta tagggcgcgg ggccgaagcc gccgccgcca
     2221 taggggaagc gatcacccat catctgggta tcgccgaaga aggtcagggt gtcgtggtag
     2281 acgccttcgg ttgccggcag ggcggcacga tcaatgccct tcagttcggc catctggccc
     2341 ctgaacaccg cccggtcggc gtcgatcttg gcctgggacc ggtcatcgag ccgggacttc
     2401 atcgccgcca tcggcccctt gtccatgccg agcgaggtca gggtctcggg cgattcc
//
LOCUS       DASVVN010000454         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_70217, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000454 DASVVN010000000
VERSION     DASVVN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70217"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1167)
                     /gene="neuB"
                     /locus_tag="VF686_07300"
     CDS             complement(70..1167)
                     /gene="neuB"
                     /locus_tag="VF686_07300"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEX8661657.1"
                     /translation="MSDPVLIIAEAGVNHDGSLDDACRMIDVAAEAGADVVKFQTFVP
                     EAVLTARAMKAAYQETNTGEGGTQLDMVRKLALSRDDHRVLADRCARRGVRFMSTAFD
                     MESLEFLSGLHMPAIKIPSGDLTWGAMLLAAARVGPPLIVSTGMATLDEVREALTVIA
                     FGLTRDGDPAGADDLERAFADGQAALREHVTLLHCTTEYPAPLEAINLRAMDTMAETF
                     GLKVGYSDHSLGLTVAVAAVARGACVIEKHFTLSRTRPGPDHPASLEPDELAAMVRAI
                     RDVEVALGRPDKAPAPAELGNLAIARRSLVAARPIRRGEILTPDAMTAKRPADGLSPM
                     EQWRLIGTAAQRDYAPDDALEAHDRDGQGTA"
     gene            complement(1160..>1524)
                     /locus_tag="VF686_07305"
     CDS             complement(1160..>1524)
                     /locus_tag="VF686_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX8661658.1"
                     /translation="NVSLHDALGPAYLPLLAACDAVVGNSSSGLAEAPSVRTPAVNIG
                     LRQAGRLAGPSVLHAEADPVAISAAIARAVAGDVPGFDNPYGDGQSSARIVSALISAP
                     PRDILLRKRFFDGEPPHV"
BASE COUNT          226 a          513 c          571 g          214 t
ORIGIN      
        1 agtcgatcga gatcagggcg tcgcggaacg tcgcctccgg cccgatcaag gctttctccc
       61 actgccgcat tacgctgtcc cctgcccgtc ccggtcgtgc gcctcgagcg catcgtccgg
      121 cgcatagtcg cgctgcgccg ccgtgccgat caagcgccat tgctccatcg gcgacaggcc
      181 gtccgcgggc cgcttggcgg tcatggcgtc gggcgtcagg atctcgccgc ggcggatcgg
      241 gcgggccgcg accaaggagc ggcgcgcgat ggcgagattg cccagttcgg ccggcgcggg
      301 cgccttgtcc gggcggccga gggcgacctc cacatcccgg atggcgcgga ccatggcggc
      361 cagttcgtcg ggctccagcg aggccgggtg atcgggtccc ggccgggtgc ggctgagggt
      421 gaaatgcttc tcgatcacgc aggcgccccg ggccacggcg gccaccgcca ccgtcagacc
      481 gaggctgtgg tcggaatagc cgaccttgag gccgaaggtt tccgccatcg tgtccatggc
      541 gcgcaggttg atggcctcca gcggggcggg atactcggtc gtgcaatgca gcagggtgac
      601 atgctcgcgc agggcggcct gaccgtcggc gaacgcgcgc tcgaggtcgt cagcaccggc
      661 ggggtcgccg tcgcgcgtca ggccgaaagc gatgacggtc agggcctcgc ggacttcgtc
      721 cagtgtcgcc atcccggtcg agacgatcag cggcggaccg acgcgcgccg ccgccagcag
      781 catggcgccc caggtcaggt cgccggacgg gatcttgatg gcgggcatgt ggaggccgga
      841 caggaactcc aggctctcca tgtcaaaggc ggtcgacatg aagcgcacgc cgcgccgagc
      901 gcagcggtcg gccagaacgc gatggtcgtc gcggctcagc gccagcttgc gcaccatgtc
      961 cagctgagtg ccgccctcgc cggtgttggt ctcctgatag gcggccttca tggcgcgcgc
     1021 ggtcagcacg gcctccggga cgaaggtctg gaatttgacc acgtccgccc cggcctcggc
     1081 ggcgacgtcg atcatccggc aggcgtcgtc cagcgatccg tcgtggttca ccccggcttc
     1141 ggcgatgatg aggacaggat cagacatggg gcggctcgcc atcgaagaaa cgcttgcgca
     1201 gcaggatgtc gcggggcggg gcggagatca gcgcagagac gatgcgcgcc gagctctgac
     1261 cgtcgccata gggattgtcg aagccgggga cgtcgccggc cacggcccgg gcgatggcag
     1321 cggagatggc gacggggtcg gcctcggcgt gcagcaccga cggcccggcc aggcgaccgg
     1381 cctgacgcag gccgatattc accgcgggag tgcgcaccga aggcgcctcc gccagaccgc
     1441 tggaggagtt gccgaccacg gcgtcgcagg cggcgagcag cggcagatag gccgggccca
     1501 gcgcgtcgtg caggctgacg ttgc
//
LOCUS       DASVVN010000455         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_387728,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000455 DASVVN010000000
VERSION     DASVVN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387728"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1326
                     /locus_tag="VF686_07310"
     CDS             343..1326
                     /locus_tag="VF686_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX8661659.1"
                     /translation="MIGFNISGWGAYRPEARLPSIVIDERLGQAPGFVEERFGIASRG
                     VAGSHETSSMMGAEACRRALARAGWNDGDFDVLIGGCGVMEQPIPSTSVLVQEKLGLG
                     GSGIPAFDVNQTCLSFLAALDVAALGLSAGRWRRAVVFSSDIASVGLDPADPKIMSIF
                     GDGAGAVAIEAAAGEAGLLSCRVESYGAGHRLAALRAGGTALRVEEGFDALVAGSRFE
                     MDAFGIFKAAAKRLPQVIDKALAEAGIDRSDLACVICHQASAPGIAHVKRLFDPVPVV
                     DIFASTGNQIAASLPTVLAHALDQDLARRGDAIMLLGTAAGISAGAMVLRL"
     gene            1323..>1808
                     /locus_tag="VF686_07315"
     CDS             1323..>1808
                     /locus_tag="VF686_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8661660.1"
                     /translation="MSRKLLITGATGGLGLGLVAAARARGHDVLATGRSDAVEGRIRG
                     EGAAFVAADLTTADLRALCRGRHTVIHAAALSASWGPRDAFERINVDATTALLDAAEA
                     EGCGRFVFVSSPSIFAGFRDRIGIRGGDTPSDPPLNHYARTKLAAERRVLSADSGAMA
                     TT"
BASE COUNT          264 a          613 c          632 g          299 t
ORIGIN      
        1 tgcgaaagca tcctgagccg ctgcatcctg aacggcggca tcccgcctat gttcaggcct
       61 cccccaccgg atcatgcggg ccatggtcga ggcaggcttg ccggaacggt gatcagttga
      121 cctggaatcc cgagtcccta cacatgagac gggcccctcg cctcggtcgc aggtggaggc
      181 agattgtctc tggactgccc ccaccaccgc ggcgcccgaa tattccagtg gttcagtgcg
      241 ggccctgctc tttagcgttg aacgcggcga cgagggcccg accttcgcac gccgtagcgg
      301 ggaaaatcct ctagggtgat tccgggcaag ggacggatcg ccatgatcgg gttcaacatc
      361 agcggttggg gcgcgtatcg cccggaagcc cgtttgccgt cgatcgtcat cgacgaacgc
      421 ctcgggcagg cgcccggctt cgtcgaggaa aggtttggca tcgccagtcg gggcgtggcg
      481 ggatcgcatg agaccagctc gatgatgggc gccgaggcgt gccggcgagc cttggccagg
      541 gcaggctgga acgatggcga cttcgacgtc ctgatcggcg gttgcggggt catggagcaa
      601 cccattccct ccacttcggt gctcgtgcag gagaagctcg gactgggcgg ctcaggcatc
      661 cccgccttcg acgtgaacca gacctgcctg tccttcctgg ccgccctgga cgtcgccgcc
      721 ctcgggctgt cggcggggcg atggcgccgc gccgtggtct tctccagcga catcgcgtcg
      781 gtcggactgg atcccgccga tccgaagatc atgtccatct tcggcgacgg cgcgggggcc
      841 gtcgcgatag aggcggcggc gggcgaagcg ggcctgttgt cctgtcgggt cgagagctac
      901 ggcgccggac atcgactggc ggcgctgcgg gcgggcggca cggccctgcg ggtcgaggaa
      961 ggtttcgatg cgctggtcgc cggctcgcgg ttcgaaatgg acgccttcgg catcttcaag
     1021 gccgcagcga agcgactgcc gcaggtcatc gacaaggccc tggccgaggc ggggatcgac
     1081 agatccgacc tcgcctgcgt gatctgccac caggccagcg cgccggggat cgcccacgtc
     1141 aaacgcctgt tcgaccccgt tccggtcgtc gacatcttcg cctcaaccgg caaccagatc
     1201 gccgcgtcgc tgccgacggt gctcgcccac gcccttgacc aggacctcgc ccgacgcggg
     1261 gatgcgatca tgttgctcgg cacggcggcg ggcatcagcg cgggcgccat ggttctgcgc
     1321 ctttgagccg caaacttctc atcaccggcg ccacgggcgg tctcggcctc ggactggtgg
     1381 ccgcggcgcg cgcgcgcgga cacgacgtcc tcgctacggg ccggtccgat gccgtggaag
     1441 gacgaatccg tggcgagggc gcggccttcg tagcggccga cctgacgacg gctgacctga
     1501 gggccttgtg ccgaggtcgc cacacggtca tccacgcggc cgcgctctct gcgtcctggg
     1561 gaccgagaga cgcgttcgag aggatcaatg tggatgcgac gacggccttg ctggacgcgg
     1621 cggaggcgga gggttgcggc cggttcgtgt tcgtctcgtc gccttcgatc ttcgccggct
     1681 tccgcgaccg gatcggcatc cgcggcggcg acacgccgtc cgacccgccg ctcaaccact
     1741 acgcccgcac caagctcgcc gccgaacgcc gggtgctgtc cgccgactcc ggtgcgatgg
     1801 cgacgact
//
LOCUS       DASVVN010000456         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_282804,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000456 DASVVN010000000
VERSION     DASVVN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282804"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..>1622)
                     /locus_tag="VF686_07320"
     CDS             complement(99..>1622)
                     /locus_tag="VF686_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8661661.1"
                     /translation="ALSFARSAGVVAALWGAGGLAVAVWLRTSRGPLYDLSFGALVAM
                     GIAAGELLAGNSTRLTVMFTVANMLEIYGCVLLARRFAPTLNLASVQGACRFLVSAAV
                     LGPLPAGLFAGGMLWWMNGADFLDSVQTWWFGHALGVAVLASFGLALTKREIVRFRAP
                     ARAIEAVVLLGMLVGLCIVVFSQQLPVGFVTMPLLLLIAVRLRMLGVTAALLIISILA
                     VGAAMRGLGPYGAAFEGADRAMMAQLLILFGYLPIMLVAALLEERDRLSDRAKAGQVR
                     AERASEAKSRLLANVAHEIKSPVGGVIGIGDLWKTGQLGPVTATQAEMAEMLVKTARQ
                     IEALAHDLLDVARAESGAVKVELRPTDVPGVLEDVKRAVALRPESEGVRLEVISEGDG
                     LIALADSQRLTQVISNLASNAVKYGGAGGVVIFRASKVYDGVRIEVSDRGPGLSPEKQ
                     AQLFEPFNRLGLERSTVEGHGIGLALAKRLVELQGGTIGVTSTPGEGAVFWVEMPAA"
BASE COUNT          302 a          561 c          542 g          217 t
ORIGIN      
        1 aataacctta gtcgtagtga ggttttacgc ccgcggtgcg ctcatgtcca tgggcgcacc
       61 ggtcgcgcgt cggaaggtcg caacggtgaa gctccgcctc aggcggcggg catctcgacc
      121 cagaagacag cgccttcgcc cggcgtcgac gtcaccccga tggttccgcc ctgcagttcg
      181 acaaggcgct tggccaaggc caggccgata ccgtggccct cgaccgtgga ccgttcgaga
      241 cccagtcggt tgaacggctc gaacagctgg gcctgtttct cgggcgacag gccgggaccg
      301 cggtcgctga cctcgatccg cacgccgtcg tagaccttgc tggcgcggaa gatgacgacg
      361 ccgccggcgc cgccgtattt gaccgcgttg gaagccaaat tggagatgac ctgggtcagg
      421 cgttgggaat cggccaaggc gatcagaccg tcgccctcgc tgatcacctc gagccgcacg
      481 ccctcggatt ccgggcgcag ggcaacggcc cgtttgacgt cttccagcac gcccggcacg
      541 tcggtggggc gaagctcgac cttcaccgcg ccggactccg cgcgggcgac atccagcaag
      601 tcgtgcgcca gcgcctcgat ctggcgggcc gtcttgacca gcatctcggc catctcggcc
      661 tgggtagccg tgacgggccc cagctggccc gtcttccaaa gatccccgat gccgatcacg
      721 cccccgacgg ggctcttgat ttcatgggcg acgttggcga gcaggcgcga cttggcttcc
      781 gaagcccgtt cggcgcgaac ctgtccggcc ttggcgcggt ccgacagccg gtcccgctct
      841 tccagcaggg ccgcgaccag catgatcggc agatagccga acaggatcag cagctgggcc
      901 atcatggcgc gatcggcccc ctcgaaggcg gcgccgtagg ggcccagccc ccgcatcgcc
      961 gcgccaacag ccagaatgga gatgatgagg agggccgcgg tgacgccgag catgcgaagc
     1021 cggacggcga tcagcagcag cagcggcatg gtgacgaagc cgaccggcaa ctgctgggaa
     1081 aagacgacga tgcacagtcc gaccagcatg cccaagagga ccaccgcctc gatcgcgcgg
     1141 gccggcgccc ggaagcggac gatctcgcgt ttggtgagcg ccagaccgaa gctggcgagc
     1201 accgcgaccc caagagcatg gccgaaccac caggtctgga cgctgtccag gaagtccgcg
     1261 ccgttcatcc accacagcat gccacccgcg aacaggccgg ccggcaaggg tccgagcaca
     1321 gccgcgctga ccagaaagcg gcatgcgccc tgaaccgagg cgagattcag cgtcggcgcg
     1381 aaacggcgcg ccagcagaac gcagccatag atctcgagca tgttggcgac ggtgaacatc
     1441 accgtcagcc gggttgagtt gcccgccagc agttcgccgg ccgctattcc catggcgacg
     1501 agagcgccga aagacagatc gtagagaggg ccgcgcgagg tccgcagcca gacagcgacg
     1561 gccagaccgc ctgcgcccca cagggcggcg accacgcccg cggaccgggc gaaactgagg
     1621 gc
//
LOCUS       DASVVN010000457         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_282898,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000457 DASVVN010000000
VERSION     DASVVN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282898"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VF686_07325"
     CDS             <1..578
                     /locus_tag="VF686_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8661662.1"
                     /translation="DHAVVGLMDPAHGPYVHQQWWWRSEHSMHEKAKAFEPRELGFAM
                     VRHAPSSNSRAYKILGGAPATEITFRLPGYRWEHIQVGQRQVLALTCLTPITETKTRI
                     TQIFWSDHWVFGIAKPFLRMGVVAFLKQDGGMVNLQNEGLRYDPALIWIDDADKQAKW
                     YQQLKREWVRSRAEGRAFANPVKPATLRWKR"
     gene            complement(696..1346)
                     /locus_tag="VF686_07330"
     CDS             complement(696..1346)
                     /locus_tag="VF686_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8661663.1"
                     /translation="MASSSPADAAEGETPRLNRRQTAKVRTRQKVLDAARQLFAERGY
                     EPATIRDIAKGAGMSTGAVFANFQDKAELFEAVLATDMVKLADTLKAAAAAETTVRGR
                     LLAALIAGYHSSLEQLPLVQAVVARSWFQPVAAEMRSREAIKPLVSVVTDALQAGVAD
                     RELRQDADVRLLGEMIYDAWLSNYRRAAYDNWTIQQLTAQIGKQVDVILAGALSRQ"
BASE COUNT          293 a          533 c          621 g          276 t
ORIGIN      
        1 tcgaccacgc ggtcgtcggc ctgatggacc ccgcccacgg cccctatgtg catcagcaat
       61 ggtggtggcg gtccgagcat tcgatgcacg agaaggccaa ggccttcgag ccgcgcgaac
      121 tgggcttcgc catggtccgc catgcgccgt cgtcgaacag ccgggcctac aagatcctcg
      181 gcggcgcgcc ggcgaccgag atcaccttcc gcctgcccgg ctatcgctgg gagcatatcc
      241 aggtaggcca gcggcaggtt ctggccctga cctgcctgac cccgattacc gagacgaaga
      301 cccggatcac ccagattttc tggtccgacc actgggtgtt cgggatcgcc aaaccattcc
      361 tgaggatggg ggtggtggcc ttcctgaagc aggacggcgg gatggtgaac ctgcagaacg
      421 agggcctgcg ctacgacccg gccctgatct ggatcgacga cgccgacaaa caggcgaaat
      481 ggtaccagca gctgaagcgc gaatgggtca ggagccgcgc agaggggcgg gcgttcgcca
      541 atcccgtgaa gcccgcgaca ttgcgctgga agagatgacg ctcttcaggg cgctgccgct
      601 tgcgacacgg tcgctcgctg cttgagcggc gctggaaaag ctgaaggccg cgacgggggg
      661 tcgcggcctt cgaaacattc ggtcgttgtg tcgccttact ggcggctgag cgcgccggcc
      721 agaatgacgt cgacctgttt gccgatctgg gccgtcagct gctggatggt ccagttatcg
      781 taggcggcgc ggcgatagtt cgacagccag gcgtcgtaga tcatctcgcc gagcaggcga
      841 acgtcggcgt cctgacgcag ctcgcggtcg gccactccgg cctggagggc gtcggtgacg
      901 accgaaacca ggggtttgat agcctcgcgc gaccgcattt cggcggcgac cggctggaac
      961 cacgagcggg cgaccacggc ctggaccagc ggcagctgct ccagcgagct gtgatagccg
     1021 gcgatcaggg cggccagcag ccggccccgc acggtggttt cggcggcggc ggcggccttc
     1081 agcgtatcgg ccagcttgac catgtccgta gccagaacgg cttcgaacag ttcggccttg
     1141 tcctggaagt tggcgaagac ggcgccggtc gacatgccgg cgcccttggc gatgtcgcgg
     1201 atggtggcgg gctcatagcc gcgctcggcg aagagctggc gcgccgcgtc cagcaccttc
     1261 tggcgggtgc ggaccttggc cgtctgtcgg cggttcagcc ggggggtttc gccctcggcg
     1321 gcgtcggcgg gggaggagga agccaaggcg gtatctcgca tgtaacgact actcaagaag
     1381 cggcggctga catccgccgg taaaaactga acgccgaagc gatggatctt acggggcgcg
     1441 cgcggcgcgc aaaatcgcct gaacggcgag gacgggcagt catcacgcaa cgatgaccaa
     1501 attggggtag ccggattaac ggagcggtta gcttgggggt taacgcggcg atggcggcgt
     1561 ttccgtcgcc tgcgtctcag cgccgcagcg tggatcgcag ggcgcgggcg cggcggggcg
     1621 cctcgctgtc aggcatggcc gcctggaaca ccttgcggaa ctggtcgcgg cgctcgtgga
     1681 tcgaggccag gaccagtccc atgggcacgc cgaggtcgat cag
//
LOCUS       DASVVN010000458         5039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_17286, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000458 DASVVN010000000
VERSION     DASVVN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17286"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VF686_07335"
     CDS             complement(<1..908)
                     /locus_tag="VF686_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8661664.1"
                     /translation="MSETPVDQLTESAAREELDRLAAELARHDALYHRQDAPEISDAE
                     YDALKRRALEIEERFPDLTTPASPSQVVGATGSSQFAPVRHGVPMLSLDNAFDPAEVI
                     DFVARIRRFLKLPADEHVAFVAEPKIDGLSANLRYENGVLITGATRGDGRTGEDVTAN
                     LRTIADIPQTLKTGENWPAVVEVRGEVYAPNDAFDAFNTAAEAAGTRTYANPRNFAAG
                     SLRQKDPAITAARPLRFFAYAWGETSEPFADTQADALKALERWGFKVNARSQRVEDTG
                     GLLALYDGLQTDRAGLGYDIDGVVYKV"
     gene            1069..2034
                     /locus_tag="VF686_07340"
     CDS             1069..2034
                     /locus_tag="VF686_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8661665.1"
                     /translation="MTHVRLAALSPLVAALFLSACATTPAALVAVAPPAPSAMHARLL
                     DDVRILAADDMQGRDTGAAGGEMARNYIVGRLDSLGVVAPPMGRLQPWEMQGRTRAGP
                     KTFNGVNVIGLIPGTRVTDRYIVVTAHYDHVGVNAEGQVFNGADDNASGVATMLALAA
                     ELKRQAPEHSVLIVALDGEERGLLGARHFVEAPPVPLTSIAMNLNFDMTARAETDGKL
                     WVTGTYQNPTFRPILQGIQPNGAVALAFGKDTPQDTGEDNWVESSDHAAFHRAQVPFL
                     YLGVNYHPDYHRPSDDFERITPSVFISATELSIASFRALDAGLDR"
     gene            2084..2632
                     /locus_tag="VF686_07345"
     CDS             2084..2632
                     /locus_tag="VF686_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661666.1"
                     /translation="MRIRTLAAVFAVLAVAACDRPAEPADAPATDAFRYTATSDVSGY
                     YKPVSEVRIGQWKLDHVFVGRSAEFRSWTGTNPDATYSPVMVELSDGASPMIETEQGR
                     VHSLMVRVHSTRYAVSDDRIEFEGEAPDTGVVRFVGRIDQGALATARRNLGDDGVVVT
                     GTLTVGNQSVRDVKLRWWLGDA"
     gene            complement(2629..4137)
                     /locus_tag="VF686_07350"
     CDS             complement(2629..4137)
                     /locus_tag="VF686_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8661667.1"
                     /translation="MPEALLSAIGEPRDVAAIDIGSNSVRLVLYRLEGRAIWTVFNEK
                     VLAGLGRELAATNHLSVEGAAQALTALKRFAAVIEGVRPAMVFVAATAAVREAADGQA
                     FCEKIAAETGLRIRVLSGEEEARYAALGVLAGIPDADGVAGDLGGSSLELVRLADGKV
                     ERGVTLPLGPFSFADEGPFDADRLREAIGKRLKTATDFRADTLYAVGGAWRTLAQVHM
                     ELNGYPLRIVHQYTIPADEMLATAALIARASRAALEKWPGVSKKRSETLPHAALVLEG
                     LVQRLGLKRVVVSAWGVREGLLYEALEPDIAAADPLLAGSSALGARQGISPTLPGALK
                     GWIAPLLAAMPVTFDKARDDVLADAACRLADLGARLHPDHRVELAFDQVLRAPVPGQT
                     HAERAYLAVAVNARYGGPVATPEPRTVSRLLTEEQRQGARALGLAIRLACDLSGRAPQ
                     LLVNARAAVKDETLVLTASDGYADVLLGEQTRRRGKALAEAMGLKLDIRNGG"
     gene            complement(4137..>5039)
                     /locus_tag="VF686_07355"
     CDS             complement(4137..>5039)
                     /locus_tag="VF686_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX8661668.1"
                     /translation="WANALERAGVQVVYGFIGWKTHAKVSMVVRREEQGYRTYCHFGT
                     GNYHPVTARIYTDLSFFTADPRVGRDAAQLFNYITGYVEPRELELLVTSPHRLRDELL
                     GLIDSEIAQARAGEPGVIWAKMNSLVDPIVIDKLYEASAAGVETDLVIRGICCLKPGV
                     PGLSDHIRVKSIVGRFLEHARIVAFANGRDLPHNKARVFISSADWMNRNLDRRVELMT
                     PVLNATVHDQVLDQIMVANLKDDAQSWVLSADGAYQRVQPADPERPFSAHKYFMTNPS
                     LSGRGRKVKTLPGQLSYEPSRKKR"
BASE COUNT          777 a         1726 c         1752 g          784 t
ORIGIN      
        1 accttgtaga ccacgccgtc gatgtcatag ccgagccccg cccggtcggt ctgaaggccg
       61 tcgtaaagcg ccagcaggcc gcccgtgtcc tcgacgcgct gcgaccgcgc attgaccttg
      121 aacccccagc gttccagcgc cttcaaggcg tcggcctggg tgtctgcgaa gggctccgac
      181 gtctcgcccc aggcgtaggc gaagaagcgt aggggccggg cggcggtgat cgccggatcc
      241 ttctggcgta gcgaaccagc ggcgaaattc cgggggttgg cgtaggtgcg cgtcccggcc
      301 gcctcagccg ccgtgttgaa ggcgtcgaag gcgtcgttcg gagcgtagac ctcgccccgg
      361 acctcgacca ccgcgggcca gttttcgccg gtcttcaggg tctgcggaat gtcggcgata
      421 gtccgcaggt tggcggtgac gtcctcgccc gtacgcccgt cgccgcgcgt cgcgccggtg
      481 atcagcacac cgttttcata gcggagattg gccgagaggc cgtcgatctt gggctcggcg
      541 acgaaggcga cgtgctcgtc cgcgggcagt ttcagaaagc gccggatacg ggcgacgaag
      601 tcgatcacct cggcagggtc gaaggcgttg tccagcgaca gcatgggaac gccatggcgc
      661 accggggcga actggctgga accggtggcg ccgacgacct gcgagggcga ggcgggggtg
      721 gtcaggtcgg ggaagcgttc ctcgatctcg agcgcgcggc gcttcagggc gtcatactcg
      781 gcgtcggaaa tctcgggcgc gtcctgacga tgatacaggg cgtcgtgccg ggccagttcg
      841 gcggccagtc tgtcgagttc ctcgcgcgcg gcggactcgg tcagctgatc gacgggggtc
      901 tcggacatgc gcggctcgcc ggaaggaatc cagcctgcgt tctagcatcg ccccgatgcg
      961 gctttcgaga ggcgacgttt attgcggtcg aacatccgga atgacggcga gttaatctga
     1021 cagcgccgtc gcgccccgct agcgtcgcgg cgattcccgg gagctcccat gacgcacgtc
     1081 cgcctcgccg ccctgtcgcc cctcgtggcg gcgctgttcc tgtcagcctg cgcgaccacc
     1141 cccgccgcgc tggtcgcagt cgcgccgccc gctccctcgg ccatgcacgc ccggttgctg
     1201 gacgacgtcc gcatcctggc cgccgacgac atgcagggcc gcgacacggg cgcggccggc
     1261 ggcgagatgg cgcgcaacta catcgtcggc cgactggaca gccttggcgt cgtcgccccg
     1321 ccgatgggcc ggctgcagcc gtgggagatg cagggccgca cccgcgcggg gccgaagacc
     1381 ttcaacggcg tcaacgtcat cggcctgatc cccggcacgc gcgtcacgga tcgctacatc
     1441 gtcgtcaccg cccactacga ccacgtcggc gtcaacgccg aggggcaggt gttcaacggg
     1501 gccgacgaca acgcctcggg cgtcgccacc atgctggccc tggccgccga gctgaagcgg
     1561 caggccccgg agcacagcgt gctgatcgtg gcgctggacg gtgaagagcg cggcctgctg
     1621 ggcgccaggc atttcgtcga ggcgccgccg gtgccgctga cctcgatcgc catgaacctg
     1681 aatttcgaca tgaccgcccg cgccgagacc gacggcaagc tgtgggtcac gggcacctac
     1741 cagaacccga cgttccgtcc gatccttcaa ggcattcaac ccaacggcgc cgtcgcgctg
     1801 gccttcggca aggacacgcc gcaggacacg ggcgaggaca actgggtcga gtcctcggac
     1861 cacgcggcct tccacagggc gcaggttccg ttcctgtatc tgggtgtgaa ctaccacccg
     1921 gactatcacc gtccgtcgga cgatttcgag cggatcacgc cctcggtctt catcagcgcc
     1981 accgagctgt cgatcgcctc gttccgggcg ctggacgccg gtctggaccg ctaggggtcg
     2041 acacgcggcg gggcgcatgc tcggtgcgtg ggtggaggaa cccatgcgca tcagaactct
     2101 cgccgccgtc ttcgccgtgc tcgccgtcgc ggcctgcgac cgtcccgccg aaccggccga
     2161 tgcgccggct acggacgcct tccgctatac cgcgacctcg gatgtatccg gctactacaa
     2221 gccggtcagc gaggtccgga tcggccagtg gaaactggac catgtgttcg tcggccggtc
     2281 ggccgagttc cggtcctgga cgggcacgaa cccggatgcg acctactcgc ccgtaatggt
     2341 ggagttgtcc gacggagcca gcccgatgat cgaaaccgaa cagggccggg tccacagcct
     2401 catggttcgg gtccattcga cgcgctacgc cgtgtcggac gaccgcatcg agttcgaggg
     2461 agaagcgccc gacacgggcg tcgtacgctt cgtcggccgg atcgatcagg gcgcactggc
     2521 gaccgcgcgc cgcaacctcg gcgatgacgg ggtggtcgtg accgggacgc tgaccgtcgg
     2581 caatcagagc gttcgtgacg tcaaactgcg ctggtggctt ggcgacgctt aaccgccgtt
     2641 gcggatatcc agcttcagcc ccatggcctc ggccagggcc ttgccgcggc gccgcgtctg
     2701 ctcgcccagc agcacgtcgg cgtagccgtc ggaggctgtc agcacgagcg tctcgtcctt
     2761 cacggcggcg cgggcgttga ccagcagctg cggcgcgcgg cccgacaggt cgcaggccag
     2821 ccggatggcc aatcccaggg cgcgggcgcc ctgacgctgt tcctcggtca gcaggcgcga
     2881 caccgtgcgc ggttcgggag tcgcgacagg cccgccgtag cgcgcgttga ccgccacggc
     2941 cagataggcg cgctcggcgt gggtctggcc cggcaccggc gcgcgcagca cctgatcgaa
     3001 ggccagttcg acgcggtggt ccggatgcag ccgggccccg aggtcggcca gccggcaggc
     3061 ggcgtcggcc agcacgtcat cgcgggcctt gtcgaaggtc acgggcatgg cggccagcag
     3121 gggggcgatc cagcccttca gcgcgccggg aagggtcggc gaaatgcctt gtcgcgcacc
     3181 gagagccgat gagccggcca gcaggggatc ggccgccgcg atatcgggct ccagcgcctc
     3241 gtacagcagg ccttcgcgga cgccccaggc tgatacgacg acgcgtttga ggcccagtct
     3301 ctggaccagt ccctcgagga ccagcgcggc gtggggcagg gtttccgacc gcttcttcga
     3361 cacgcccggc catttctcga gcgccgccct ggacgcccgc gcgatcaggg ccgcggtcgc
     3421 cagcatctcg tccgcgggta tcgtatactg gtgcacgatg cgcagcggat agccgttcag
     3481 ctccatatgc acctgggcca aggtccgcca cgctccgccg acggcgtaaa gggtatccgc
     3541 cctgaaatcg gtcgccgtct tcagccgctt gccgatcgcc tcgcgcagcc ggtcggcgtc
     3601 gaaagggccc tcgtcggcga aggagaaggg cccgagaggc agggtcacgc cgcgttcgac
     3661 cttgccgtcc gccagacgca ccagttccag gctggagccg cccagatcgc cggccacccc
     3721 atcggcgtcc ggaatgcccg ccagaacgcc gagggcggcg tagcgtgcct cctcctcgcc
     3781 cgacaggacg cggatccgca ggccggtctc ggcggcgatc ttttcgcaga aggcctgccc
     3841 gtcagcagcc tcgcgcaccg ccgcggtggc cgcgacgaag accatggccg gacgcacgcc
     3901 ctcgatcacg gccgcgaacc gcttcagcgc cgtcagcgcc tgagcggcgc cctcgaccga
     3961 cagatggttg gtggcggcca gttcgcggcc aaggccggcc aacacctttt cgttgaacac
     4021 ggtccagatg gcgcggcctt ccagccggta caggaccaga cggaccgagt tcgagccgat
     4081 gtcgatggcc gcgacgtcgc gcggctcccc gatcgccgac agcagggcct cgggcatcag
     4141 cgcttcttcc gcgagggctc gtagctgagc tggccgggca gggtcttcac cttgcgtccg
     4201 cgtcccgaca ggcttgggtt ggtcatgaag tatttgtggg ccgagaaggg ccgttccgga
     4261 tcggccggct ggacgcgctg ataggcgccg tcggcggaca atacccagct ctgggcgtcg
     4321 tccttcagat tggcgaccat gatctggtcc agcacctggt cgtggacggt ggcgttcagc
     4381 acgggggtca taagctcgac ccggcggtcg aggttccggt tcatccagtc ggccgacgag
     4441 atgaagaccc tggccttgtt gtgcgggagg tcgcggccat tggcgaaggc gacgatgcgg
     4501 gcgtgctcga ggaagcggcc gacgatcgac ttgacccgga tatggtccga gaggcccggc
     4561 accccgggct tcaggcagca gatgccccgg atgaccagat cggtttccac gcccgcggcg
     4621 ctggcctcgt agagcttgtc gatgacgatc gggtcgacca gcgagttcat cttcgcccag
     4681 atcacccccg gctccccggc ccgggcctgg gcgatctcgg agtcgatcag gccgagcagc
     4741 tcgtcgcgca gacggtgcgg cgaggtgacc agcaactcca gctcgcgcgg ctcgacatag
     4801 ccggtgatgt agttgaacaa ttgcgcggca tcgcggccca cccgcggatc ggcggtaaag
     4861 aagctcagat cggtgtagat ccgggcggtg acggggtgat aattgccggt gccgaaatgg
     4921 cagtaggtgc gatagccctg ctcctcgcgg cggacgacca tcgacacctt ggcatgggtc
     4981 ttccagccga tgaagccgta gacgacctgg accccggccc gctcgagcgc attggccca
//
LOCUS       DASVVN010000459         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_363258,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000459 DASVVN010000000
VERSION     DASVVN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363258"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VF686_07360"
     CDS             <1..747
                     /locus_tag="VF686_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8661669.1"
                     /translation="APAADAAAAPEPAVVGGADHGKLTPAAMFMQADPVVKVVMVGLL
                     LASVFSWVLLLFKIFEFGSLNRKTDVFLENFRQARTIADMRRVATSDEFDGNPLADMA
                     AAATDEIELSRQAGLSVTGDHADSALMRSQQAVGAVQAGLAKRLSGGQQFLASVGSTG
                     PFIGLFGTVYGIMNSFIGIANTNTTNLAVVAPGIAEALLATGIGLFAAIPAVIFYNYF
                     NTRISAYGTRSDGFAAELTNAISRNLDKGA"
     gene            753..1196
                     /locus_tag="VF686_07365"
     CDS             753..1196
                     /locus_tag="VF686_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8661670.1"
                     /translation="MAAKLSGGGGGKYQVEQNSEINVTPFVDIMLVLLIIFMVAAPLA
                     TPSVEVKLPRAVAPPAQNPPVPIYISIQSNGRAYLGDFPVELDQIGPELTGQIGRRNP
                     ADERIFIRADRTTRYGDFMQVMNSLQDNGFYSVALVGEDTKAPGT"
     gene            1193..1912
                     /locus_tag="VF686_07370"
     CDS             1193..1912
                     /locus_tag="VF686_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8661671.1"
                     /translation="MTDVEYHRSRYDAPKAKGLFGVPPGVLLGSLLIVSLLFAVLAFY
                     FINARFKLKEINYSDEAVDVEIIEPIPPPPPPPPPPPPPDTPPPPKLQVRPPVVVPNA
                     PPPPVTLPIEPTKKEDRVEYTGPPVIVPGPPPPPAPPAPPRPAVINNVTWGRQPAPEF
                     PERAQSRGIESGTATLLCTVGANGSATNCSIVSEDPAGAGFGQAALRSMSRARFSPRT
                     VDGVAQGGQARFTVRFRLADE"
     gene            2026..>2233
                     /locus_tag="VF686_07375"
     CDS             2026..>2233
                     /locus_tag="VF686_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEX8661672.1"
                     /translation="MRSLAAAAAALLACSFALGGCAYNETLGRNQLLIVDDSSLAQQS
                     DAAWAEALRTQRVSSDAAGNARIRR"
BASE COUNT          345 a          806 c          689 g          393 t
ORIGIN      
        1 gctccggccg ctgacgccgc cgccgctccc gagcccgccg tcgtcggtgg cgccgatcat
       61 ggcaagctga cgcccgccgc catgttcatg caggccgacc cggtcgttaa ggtcgtgatg
      121 gtgggcctcc tgctcgcctc ggtcttctcc tgggtgctgc tgctgttcaa gatcttcgag
      181 ttcggctcgc tgaaccgcaa gacggacgtg ttcctcgaga acttccgtca ggcccgcacc
      241 atcgccgaca tgcgccgcgt ggccacgtcg gacgagttcg acggcaaccc gctggccgac
      301 atggccgccg cggcgaccga cgaaatcgaa ctgtcgcgtc aggctggcct ctcggtcacc
      361 ggcgatcacg ccgactcggc cctgatgcgt tcgcaacaag ccgtcggcgc cgttcaggcc
      421 ggtctggcca agcgtctgtc gggcggccag cagttcctcg cctcggtggg ttcgaccggt
      481 ccgttcatcg gtctgttcgg caccgtttac gggatcatga actccttcat cggcatcgcg
      541 aacaccaaca cgaccaacct ggccgtcgtc gcccccggta tcgccgaggc gctgctggcc
      601 accggtatcg gtctgttcgc cgctatcccg gccgttatct tctacaacta cttcaacacc
      661 cgcatctcgg cgtacggcac ccgttcggac ggcttcgccg ccgagctgac caacgccatc
      721 tcgcgtaacc tcgacaaggg agcgtaagcc agatggccgc caaactttca ggcggcggcg
      781 gcggcaagta tcaggtcgag cagaacagcg agatcaacgt tacgccgttc gtggatatca
      841 tgctggttct gctcatcatc ttcatggtgg cggccccgct tgctaccccg tcggtcgaag
      901 tgaagctccc gcgtgctgtc gccccgcccg cccagaaccc gccggttccg atctatatct
      961 ctatccagag caacggtcgg gcttaccttg gcgacttccc tgtggaactg gaccagatcg
     1021 gtccggagct gacggggcag atcggccgtc gcaatcccgc ggacgaacgg atctttatcc
     1081 gtgcggaccg gaccacccgc tacggcgatt tcatgcaggt catgaactcg ctgcaggaca
     1141 acggcttcta cagcgttgcc ctggtgggtg aagacacgaa ggctccgggc acatgacaga
     1201 cgtagaatat caccgttcca ggtatgacgc gccgaaggcc aaggggctgt tcggcgtgcc
     1261 gccgggggtt ctgctcggca gcctgctgat cgtctccctg ctcttcgccg tcctcgcgtt
     1321 ctacttcatc aacgcgcggt tcaagttgaa ggagatcaac tactcggacg aggccgtcga
     1381 cgtcgagatc atcgagccga tcccgccgcc gccgccgccg cccccgccgc cgccgccgcc
     1441 ggacacgccc ccgccgccca agctgcaggt gcgtcccccg gtcgtcgttc cgaacgctcc
     1501 gccgcctccg gtgacgttgc cgatcgaacc gacgaagaag gaggaccggg tcgaatatac
     1561 gggtccgccc gtcatcgtcc ccggaccgcc gccgcctccg gctcctccgg ctccgccgcg
     1621 tcccgccgtc atcaacaacg tgacgtgggg ccgtcagccc gctccggaat tcccggaacg
     1681 cgctcaaagc cgcgggatcg agtcgggcac ggcgacgctg ctgtgcaccg tcggcgccaa
     1741 cggctcggcc acgaactgca gcatcgtgtc ggaagatccg gccggtgcgg gcttcggcca
     1801 ggccgcgttg cgctcgatgt cccgggcccg gttctcgccc cgcaccgtcg acggggtggc
     1861 tcagggcggc caggcgcggt tcacggttcg cttccgcctc gcggacgagt aggacctcga
     1921 gaccacgacc aaggaacacc ccgggggaaa cctcggggtg tttttctttg agcggagcct
     1981 ttccgggcgc cggacattat gggggcgctg ttcaggagac accccatgag atcgctcgcc
     2041 gccgccgccg ccgccctgct ggcctgttcc ttcgccctgg gcgggtgcgc ctataacgag
     2101 accctcggcc gcaaccagtt gctgatcgtc gacgacagtt ccctggcgca acagtccgac
     2161 gcggcatggg cggaggccct gcgcacccag cgcgtctctt cggacgccgc cggcaatgcc
     2221 cgcatccgcc gtg
//
LOCUS       DASVVN010000460         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_412379,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000460 DASVVN010000000
VERSION     DASVVN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412379"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF686_07380"
     CDS             complement(<1..375)
                     /locus_tag="VF686_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8661673.1"
                     /translation="MDTLADRWARLEPHATIVGPDDDRPRPAVILFHGCGGLRDHLPR
                     YAAAAVAAGWRAVIVDSYAARGWSRQFALAMVCTGILLHGSQRAGDVLAALHGVSQRR
                     DVDVSKVVLAGWSHGGWGIMEAM"
     gene            complement(421..885)
                     /locus_tag="VF686_07385"
     CDS             complement(421..885)
                     /locus_tag="VF686_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8661674.1"
                     /translation="MTDIRIEAADPQDAEARGLLDALSATLETITGASGRASFDPEDV
                     RGSGACFLLARDAADQAVGCGAYRPLTPGVAEIKRMYAAPGTRGVGAAVLAALEQRAR
                     ADGYGETRLETRRVNVRAVAFYDRSGYAPVAPWGPYVGRPDAVCFGKALLHD"
     gene            complement(882..2486)
                     /gene="purH"
                     /locus_tag="VF686_07390"
     CDS             complement(882..2486)
                     /gene="purH"
                     /locus_tag="VF686_07390"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8661675.1"
                     /translation="MPAAPDFPPAPDRVAPKRALISLSDKTGLEDAARTLHGLGVELV
                     STGGTRAAIAGFGVPVKDVADLTGFPEMMDGRVKTLHPVVHGGLLGVRGAADHAEAMS
                     THNIGPIDIVWVDLYPFEATVASGGGFDAVVENIDIGGPAMIRSGSKNHGYVAVCVDP
                     ASVAELLEALRADGVTTLELRKRLAARAFARTAAYDAAVSGWFAGQVGDAAPARKSVA
                     GSLAQTLRYGENPHQTGAFYRTGETRPGVAHARQLQGKELGYNNIADADAAYELVAEF
                     EAPACVIVKHANPCGVAVGNDLTAAYARALECDAVSAFGGVVAVNRPLSGADAKLITD
                     IFTEVVIAPGADEEAKSIFAAKRNLRLLLTDGLPDPLAAGEVFRSVAGGFLVQSRDRS
                     RIAASDLKIVTRRQPTPQEIEDMLFAFTVAKHVKSNAIVYAKDGQTAGIGAGQMNRRD
                     SARIAAIRAREAGEAKGLAHSLAQGSACASEAFFPFADGLLEAVAAGATAVIQPGGSI
                     RDDEVIAAADEAGVAMAFTGVRVFRH"
BASE COUNT          369 a          873 c          998 g          341 t
ORIGIN      
        1 catggcctcc atgatgcccc agccgccgtg actccagccg gcgagaacga cctttgagac
       61 gtccacatcc cgccgctgtg acacgccgtg cagggcggcg agcacgtcgc cggccctttg
      121 cgagccgtgc agcagtatgc cggtgcagac catggcgagg gcgaactggc ggctccagcc
      181 gcgcgccgca taggaatcta caatgaccgc ccgccatccc gcggcgaccg cggcggcggc
      241 gtagcggggc aggtgatcgc gcaggccgcc gcagccgtga aacaggatca cggccgggcg
      301 cggccgatcg tcgtccgggc cgacgatggt ggcgtggggc tcgaggcggg cccagcggtc
      361 ggcgagagta tccatgcgcc gatctagtcc ggcgagcggc cggaaggcca gagcctgcgc
      421 tcaatcgtgc agcagcgcct tgccgaaaca gacggcgtca gggcggccga cgtacgggcc
      481 ccagggcgcg acgggcgcat agccggaccg gtcgtagaag gcgacggcgc ggacgttgac
      541 ccgccgcgtc tcaagccggg tttccccgta gccgtcggcg cgcgcccgct gttcgagggc
      601 ggcgagaacg gccgcgccga cgccgcgcgt tccgggcgcc gcatacatcc gcttgatctc
      661 ggccaccccg ggcgtcagcg ggcgataggc gccgcagccc acggcctgat cggctgcgtc
      721 acgcgccagc aggaaacagg cgccggagcc ccggacgtct tccggatcga aggacgcccg
      781 cccgctcgcc cccgtgatcg tctcgagcgt cgccgacagg gcgtccagca ggccgcgcgc
      841 ctccgcgtcc tgcggatcgg cggcctcgat gcggatgtcg gtcagtgccg gaagacccgc
      901 acgccggtga aggccatggc cacgccggcc tcgtcggcgg cggcgatgac ctcgtcgtcg
      961 cggatcgagc cgccgggctg gatcaccgcc gtggccccgg ccgcgaccgc ttccagcagg
     1021 ccgtcggcga aggggaagaa ggcttcggag gcgcaggcgg agccctgggc cagagagtgg
     1081 gccagaccct tggcctcgcc ggcctcgcgg gcgcggatgg cggcgatgcg ggccgagtcg
     1141 cggcggttca tctggccggc gccgatgccg gcggtctggc cgtccttcgc atagacgatg
     1201 gcgttggact tgacgtgctt cgccacggtg aaggcgaaca gcatgtcctc gatctcctgc
     1261 ggagtcggct gacggcgggt gacgatcttg agatccgagg cggcgatacg gctgcggtcg
     1321 cgcgactgga ccaggaagcc gccggccacc gagcggaaca cctcgccggc cgccagcgga
     1381 tcgggcaggc cgtcggtcag cagcaggcgc aggttcctct tcgcggcgaa gatggatttc
     1441 gcctcctcat cggcgcccgg ggcgatgacc acctcggtga agatgtcggt gatcagtttc
     1501 gcatcagcgc ccgacagcgg ccggttgacg gcgaccacgc cgccgaaggc cgagacggcg
     1561 tcgcactcca gggcgcgggc gtaggcggcc gtcaggtcat tgccgacggc gacgccacac
     1621 gggttggcgt gtttgacgat gacgcaggcg ggagcctcga actcggcgac cagctcatag
     1681 gccgcgtcgg cgtcggcgat gttgttgtag cccagctcct tgccctgcag ctggcgggcg
     1741 tgggcgacgc ccggccgcgt ctcgccggtg cggtagaagg cgcccgtctg atgcgggttc
     1801 tcgccgtagc gcagggtctg ggccagcgag ccggcgacgg acttgcgggc cggggcggcg
     1861 tccccgacct gaccggcgaa ccagccggag acggcggcgt cataggcggc ggtgcgggcg
     1921 aaggcgcggg cggcgaggcg tttgcgaagc tcgagggtgg tgacgccgtc ggccctgagc
     1981 gcctccagca gttcggcgac cgaggccggg tcgacgcaga cggcgacata gccgtggttc
     2041 ttggaccccg agcggatcat ggccgggccg ccgatgtcga tgttctcgac cacggcgtcg
     2101 aagcctccgc cggacgccac ggtggcctcg aacggataga ggtcgaccca gacgatatcg
     2161 atggggccga tgttgtgggt gctcatggcc tcggcgtggt cggccgcgcc gcgcacgccc
     2221 agcaggccgc cgtggaccac ggggtgcagg gtcttgaccc ggccgtccat catctcgggg
     2281 aagccggtca gatcggccac gtccttcacc ggcacgccga agccggcgat ggcggcgcgg
     2341 gtgccgccgg tcgagaccag ttcgaccccg aggccgtgca gggtgcgggc ggcgtcctcc
     2401 agcccggtct tgtcggacag ggagatcagg gcgcgcttcg gcgccacgcg gtccggcgcg
     2461 gggggaaagt cgggagcggc gggcatggcg gcgtccgtgt ctggcgtgag gtcggggaga
     2521 cgcgggggct ctagcaccgg atgcgccggg ctggaaccgg ggaggtcaga tcaggccgga
     2581 c
//
LOCUS       DASVVN010000461         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_176430,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000461 DASVVN010000000
VERSION     DASVVN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176430"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF686_07395"
     CDS             <1..322
                     /locus_tag="VF686_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8661676.1"
                     /translation="REGADWLTDAQLADLESALNLRNEGNAEVLFAWLQIAVQHRYQP
                     AVPTLEHFLTTQGRRKFVLPLFTSLWAKGDWGRPIARRIYARARPGYHPVTTGSVDAV
                     VGRP"
     gene            326..1633
                     /locus_tag="VF686_07400"
     CDS             326..1633
                     /locus_tag="VF686_07400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8661677.1"
                     /translation="MHLDRRRMIAASAALSLIGGAAAWRRQDDALTALADPTRPDTVL
                     GFGVIARDGAGRTVLERVQGEGRLTVNGSLRTRPFTLETPMRIASITKLVAMTALMTL
                     VEQGRLSLDDDAGDLAGFPLRHPAHSDVRITPAMLASHTSGLRNGPSYPVPLGRRLSE
                     AFTDGGRHFDDGGWFAPASEPPGFFTYADVNFAVLAQIAERVSGERFDRFLKRAVLEP
                     VGIEAGLNWSGVSDATRARASAACRLTNGVWEPQVDAEVFPAPAIRVTTPPEAPGLTA
                     DGYVVGDNGFAFSPQGGLRASVRDLDRMARSYAGHAEAPQVLSRATLDRMGAPIWTYN
                     PSRRNGHTDRGLLQSYGLSVHIPTSRDGDAFFGPGSADWRGHFGEAYGLQSGLFWNRR
                     DGRTLTYMISGTPRPAEGLSGARSAASPWEEVILDAALAAWAG"
     gene            1743..>2522
                     /locus_tag="VF686_07405"
     CDS             1743..>2522
                     /locus_tag="VF686_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX8661678.1"
                     /translation="MHDAPQSEAAARLDKENPLGVDGFEFVEFTGPDPAAMVSRLELM
                     GFTQTHVNPANDVVRLKQGDITLLVNRSPSGQAGEFATEHGPSANGMAFRVRDAKAAY
                     EGAVARGARPAENHNGGALAGGYPYILQGIGGSLLYIIDQYGEQGSLYDGWNEIEGWE
                     EAERKNSVGLFMLDHLTHNVRRGEMRTWSGFYGDVFAFEEQKYFNIKGKATGLLSQAM
                     IAPDRAIRIPLNESQDDSSQIEEFLRRYNGEGIQHIALATDN"
BASE COUNT          396 a          910 c          835 g          381 t
ORIGIN      
        1 ccgcgagggc gccgactggc tgaccgacgc ccagctggcc gacctggaat cggcgctgaa
       61 cctgcgcaac gagggcaatg ccgaggtgct gttcgcctgg ctgcagatcg cggtccagca
      121 ccgctatcag cccgccgtgc cgacgctgga gcatttcctg accacccagg gccggcgcaa
      181 attcgtcctg ccgctgttca ccagcctgtg ggccaagggc gactggggcc gtccgatcgc
      241 gcggcgcatc tacgcccggg cgcgacccgg ctatcacccg gtgaccaccg gctcggtcga
      301 cgcggtggtg ggccggccct aggccatgca tctggatcgc cgccgcatga tcgccgcgag
      361 cgccgccctt tcgctgatcg gcggtgcggc ggcatggcga cggcaggatg acgcgctgac
      421 ggcgctggcc gatccgacaa ggccggacac ggtcctgggc ttcggcgtca tcgctcgcga
      481 cggcgccggg cggacggtgc tggagcgggt gcagggcgaa ggccggttga ccgtgaacgg
      541 atcgctgcgg acacggccgt tcacgcttga gaccccgatg cgcatagcct cgatcaccaa
      601 gctggtggcg atgacggcgc tgatgaccct ggtcgaacag ggccggctat cgctcgacga
      661 cgacgccggc gatctggccg gcttccctct gcgccatccc gcacattccg atgtgcgcat
      721 aacgccggcc atgctggcca gccacacctc aggtctgaga aacgggccca gctatcctgt
      781 gccgctgggc cgccgtctgt ccgaggcctt caccgatgga gggcggcatt tcgacgacgg
      841 cggctggttc gcgccggcgt ccgaaccgcc cggcttcttc acctacgccg acgtcaattt
      901 cgccgtgctc gcccagatcg ccgaacgcgt gtccggcgag cggttcgacc ggttcctgaa
      961 acgcgccgtg ctggaacccg tcggaatcga ggccggcctg aactggagcg gcgtgtcgga
     1021 cgcgacccgc gcacgcgcct ccgccgcctg ccgtctcact aacggcgtct gggagccgca
     1081 ggttgacgcg gaggtctttc cggcccccgc gatccgggtc accaccccgc ccgaggcgcc
     1141 gggcctgacc gcggacggct atgtcgtcgg cgacaatggc ttcgccttct cgccgcaggg
     1201 cggattgcgg gcgtccgtgc gcgacctcga ccggatggcc cggtcatacg ccggccatgc
     1261 cgaagcgccg caggtgctct cgcgcgccac gctggaccgc atgggcgcac cgatctggac
     1321 gtacaatcca agccgccgca acggccatac tgaccgcggt ctgctccaga gctatggcct
     1381 gtcggtgcac atccccacga gccgggatgg cgacgccttc ttcggtcccg gcagcgccga
     1441 ctggcgcggg cattttggcg aggcctacgg cctgcaatcc ggcctgttct ggaaccgccg
     1501 cgacggccgc accctgacct atatgatctc cggcaccccc cgtccggcgg aaggcctgtc
     1561 cggcgcacgc tcggccgcct cgccgtggga ggaggtcatc ctcgacgctg ctctggctgc
     1621 gtgggccgga tgacgcggcc gtgagcgcgc cgcgccttcc cttcgagcct ccgcgcccct
     1681 aggttccgcc ctcaagaacg gcccggacaa agacacgggc cgacacgctc aggagacgcc
     1741 ccatgcatga cgcgccccaa tccgaagcgg ccgcccgcct cgacaaggaa aaccccctcg
     1801 gcgtcgacgg cttcgagttc gtggagttca ccggcccgga tcccgcggcc atggtttcgc
     1861 gcctcgagct gatgggcttc acccagacgc atgtgaaccc ggccaatgac gtggtgcggc
     1921 tgaagcaggg cgacatcacc ctgctggtca accgctcgcc ctccggtcag gcgggcgagt
     1981 tcgccaccga gcacgggccc tcggccaacg gcatggcctt ccgcgtgcgc gatgcgaaag
     2041 cagcctatga aggcgcggtg gcgcgcggcg cgcgtccggc cgagaaccac aacggcggcg
     2101 ccctggccgg cggctatccc tatatcctgc agggcatcgg cggctcgttg ctttacatca
     2161 tcgaccagta cggcgagcag ggctcgctct acgacggctg gaacgagatc gagggctggg
     2221 aggaggccga gcgcaaaaat tcggtcggtc tgttcatgct cgaccacctg acccacaacg
     2281 tgcgccgcgg cgagatgcgc acatggtccg gcttctacgg cgacgtcttc gccttcgagg
     2341 agcagaaata tttcaacatc aagggcaagg cgaccggcct gctgtcccag gcgatgatcg
     2401 cgcccgatcg cgccattcgc atccccctga acgagagcca ggacgacagc tcacagatcg
     2461 aggagttcct gcgccgctac aacggtgagg gcatccagca catcgccctg gccaccgaca
     2521 ac
//
LOCUS       DASVVN010000462         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_412501,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000462 DASVVN010000000
VERSION     DASVVN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412501"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF686_07410"
     CDS             complement(<1..269)
                     /locus_tag="VF686_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8661679.1"
                     /translation="MSTRPEIDTGMEGFRFWPGALSASEQAGLLAEVLAAEAQAPFYQ
                     PVTPGGRPFSVRMTSLGPLGWVSDRAGYRYQATHPVTGAAWPPMPG"
     gene            complement(376..789)
                     /locus_tag="VF686_07415"
     CDS             complement(376..789)
                     /locus_tag="VF686_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661680.1"
                     /translation="MSVQKTIARAALILAALASTAAAAATTTVAPERAVAATAATASA
                     PVQSRDGRNRRVRVHNSTGWTMLRFYASNTSRSDWEEDILGDDVLANNASIVMNIDDG
                     TGACMFDFKAVFTNGQELVRNNVNVCEIADYYYTR"
     gene            1094..3016
                     /gene="dnaK"
                     /locus_tag="VF686_07420"
     CDS             1094..3016
                     /gene="dnaK"
                     /locus_tag="VF686_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8661681.1"
                     /translation="MAKIIGIDLGTTNSCVAVMDGKNPKVIENAEGNRTTPSVVAIQD
                     SGEVLVGQPAKRQAVTNPTNTFFAIKRLIGRNFTDPVVAKDKGMVPYEIVKGPTGDAW
                     VKAHGKDYSPQQVSAFTLGKMKEAAEAYLGETVTQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKNDGQKIAVYDLGGGTFDVSILEIGDGVFEVKSTNG
                     DTFLGGEDFDLRLVDFLADEFKKEQGVDLRSDKLALQRLKEEAEKAKKELSSTTQYEV
                     NLPFITMNASGPLHLNIKLSRSKLEALVEDLVQRTIEPCAKALKDAGMKASDIDEVVL
                     VGGMTRMPMVQEAVKKFFGKEPHKGVNPDEVVALGAAVQAGVLQGDVKDVLLLDVTPL
                     TLGIETLGGVFTPLIERNTTIPTKKSQVFSTADDNQSAVTIRVFQGERPMAADNKVLG
                     QFDLMGIPPAPRGMPQIEVAFDIDANGIVHVTAKDKATNKEQSIRIQANGGLSDADID
                     KMVKEAEANAAADKAKKEMVEAVNAADSLIHSTEKAMAEHGDKVGAEEKTAIETALEE
                     LKAAKEGSDPEDIRTKTNTLVQASMKLGEAMYASQQGAGDGASDEAPADDGVVDAEFE
                     EVSPDADASDDEKKSA"
     gene            3187..4371
                     /gene="dnaJ"
                     /locus_tag="VF686_07425"
     CDS             3187..4371
                     /gene="dnaJ"
                     /locus_tag="VF686_07425"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8661682.1"
                     /translation="MATRDYYEVLGVERTIDAAGLKAAYRKLAMQHHPDRNGGCDDSM
                     ARFKEISEAYSVLSDDQKRAAYDRFGHAGVNGGGGGNPFGQGQGFADVNDIFSQVFGE
                     AFGDVFGGRAGGGARQHGPRRGSDLRYDLEITLEQAYKGADVEISVPTTATCDTCEGT
                     GAKAGTKPSTCTTCQGAGRVRSANGFFQVERTCPRCGGHGQMIAEPCASCAGHGQVRK
                     NRKLQLKVPAGVDDGSRIRLSGEGDAGTRGGPRGDLYVFLSVTPHELFERDNLDLLVT
                     VPVPMTTAALGGEIDAPCLTSEACDGRCRAPVTVPAGAQTGKTVRIKGKGMPHLNGKN
                     RGDLVVELFVETPTDLTPHQKELLQELAASLGESQTPRNSSFAGKAKRFWADILGSEA
                     PQ"
BASE COUNT          879 a         1572 c         1451 g          646 t
ORIGIN      
        1 cccggcatcg gcggccaggc cgcgcccgtg accggatgcg tcgcctgata gcggtagcct
       61 gcccggtccg acacccagcc cagcggcccc agactggtca tccgcaccga gaacggccgt
      121 ccgccgggcg tcacgggctg atagaacggc gcctgcgcct cggccgccag cacctcggcc
      181 agcaatcccg cctgttcaga cgcggaaagg gctcccggcc agaaccggaa gccctccatt
      241 cccgtatcga tctcaggacg cgtggacaca gcccaaccta ggctccgtcg tcccgcgctc
      301 aagtcgcgcg aagcgcggca acgccccaaa acgcctcaag tagcccgaag ggcggtaggc
      361 ccaataggaa gccgatcagc gcgtgtagta ataatccgcg atctcgcaga cgttgacgtt
      421 gttgcggacc agctcctggc cgttggtgaa cacggccttg aagtcgaaca tgcaggcgcc
      481 ggtgccgtcg tcgatgttca tcacgatgct ggcgttgttg gccaggacgt cgtcgcccag
      541 aatgtcctct tcccagtcgc tgcggctggt gttggaggcg tagaagcgca gcatggtcca
      601 gccggtcgag ttgtgaaccc gcacgcggcg gttccggccg tcgcgcgact ggacgggcgc
      661 ggaagcggtg gcggcggtgg cggccacggc ccgttcgggc gcaacggtgg tcgtggcggc
      721 ggctgccgcg gtcgaagcca aggctgccag aatcaaggcg gcgcgggcga tggtcttttg
      781 aacgctcatg tcggtctctc cccgaatacc ggccccccaa ggtcggtgtg acgtctatta
      841 actacaccaa tgttcggcga aatcatccat tctgatgaag gggcgaatct tctcgcaaaa
      901 gcgtttgggg cttgcagggc ggggggtgtt tcccatatcg gccacaaccg ccgtaaatcc
      961 ccgcagttcg tggggctttg cgccggaacc gtaccaaccg accaaagacc ctgacaccgg
     1021 gggcggtcac gcgcggcgct tcgctttaaa aggccgccgc atcgcccgct gaaatggagt
     1081 gaaagaccaa cacatggcca agatcatcgg catcgacctg ggcacgacga actcgtgcgt
     1141 cgccgtcatg gacggcaaga accccaaggt catcgaaaac gccgagggca accgcaccac
     1201 gccctccgtc gtcgctatcc aggacagcgg cgaagtgctg gtgggccagc cggccaagcg
     1261 ccaggcggtc accaacccga ccaacacctt cttcgccatc aagcgcctga tcggtcgcaa
     1321 tttcacggat cccgtggtgg ccaaggacaa ggggatggtc ccctatgaga tcgtcaaggg
     1381 cccgaccggc gacgcctggg tgaaggccca cggcaaggac tattcgcccc agcaggtctc
     1441 ggccttcacg ctcggcaaga tgaaggaggc cgcggaggcc tacctcggcg agaccgtgac
     1501 gcaagccgtc atcaccgtgc ccgcctattt caacgacgcc cagcgtcagg cgaccaagga
     1561 cgccggcaag atcgccggtc tggaagtcct gcgcatcatc aacgagccga ccgcggccgc
     1621 cctcgcctat ggcctggaca agaacgacgg ccagaagatc gccgtctatg acctcggcgg
     1681 cggcaccttc gacgtctcga tcctcgagat cggcgacggc gtgttcgagg tgaagtcgac
     1741 caacggcgac accttcctcg gcggcgagga cttcgacctg cgcctcgtcg acttcctggc
     1801 cgacgagttc aagaaggagc agggcgtcga cctgcgctcc gacaagctcg cgctccagcg
     1861 cctcaaggaa gaggccgaaa aggccaagaa ggagctgtcc tcgaccaccc agtacgaggt
     1921 caacctgccg ttcatcacca tgaacgcctc gggcccgctg cacctgaaca tcaagctgtc
     1981 gcgctccaag ctggaggccc tcgtcgagga cctggttcag cgcaccatcg agccctgcgc
     2041 caaggcgttg aaggacgcgg gcatgaaggc ctcggacatc gacgaagtgg tgctggtcgg
     2101 cggcatgacc cgcatgccta tggttcagga agccgtgaag aaattcttcg gcaaggagcc
     2161 ccacaagggc gtgaaccctg acgaggtcgt ggccctcggc gctgccgttc aggccggcgt
     2221 tctgcagggc gacgtcaagg acgtgctgct gctggacgtg accccgctga ccctcggcat
     2281 cgagaccttg ggcggcgtct tcaccccgct gatcgaacgc aacaccacca tcccgaccaa
     2341 gaagagccag gtcttctcga ccgccgacga caaccagtcg gccgtgacga tccgggtctt
     2401 ccagggcgag cgtccgatgg ccgccgacaa caaggtgctg ggccagttcg acctgatggg
     2461 catcccgccc gcgccgcgcg gcatgccgca gatcgaggtc gccttcgaca tcgacgccaa
     2521 cggcatcgtc cacgtgacgg ccaaggacaa ggcgaccaac aaggaacagt cgatccgcat
     2581 ccaggccaac ggcggccttt ctgacgccga catcgacaag atggtcaagg aagccgaggc
     2641 caacgccgcg gccgacaagg cgaagaagga aatggtcgag gcggtcaacg ccgccgacag
     2701 cctgatccac tcgaccgaaa aggccatggc cgaacacggc gacaaggtcg gcgcggaaga
     2761 gaagaccgcc atcgaaaccg cccttgagga gctcaaggcg gccaaggaag gttcggatcc
     2821 ggaagacatc cggaccaaga ccaacaccct ggtccaggcc tcgatgaaac tgggcgaggc
     2881 catgtacgcc tcgcagcagg gcgccggcga cggcgcctcg gatgaagccc ccgccgacga
     2941 cggcgtggtc gacgccgagt tcgaggaagt ctcgcctgac gccgacgcgt ccgacgacga
     3001 gaagaagagc gcgtaagcca caccgctcgg acaatcaccc ggccccgctt gtctgacgac
     3061 gggcggggcc gtttcatgaa cttgcatcat ttccccgggc caccatttca gcccggtcag
     3121 ccgcaaaggt tatcggcccg ccaagaggcc ctgaccgccg gcgaagagta agaggacgac
     3181 cgccgcatgg cgacgcgtga ctattacgag gttttggggg tcgagcggac catcgacgcc
     3241 gccggcctga aggccgccta tcgcaagctg gccatgcagc accaccccga tcgcaacggc
     3301 gggtgcgacg actccatggc ccggttcaag gaaatctccg aagcctattc cgtcctgtcc
     3361 gacgatcaga agcgcgctgc ctacgaccgc ttcggccacg ccggggtcaa cggcggcggc
     3421 ggcggcaatc cgttcggcca gggccagggc ttcgccgacg tcaacgacat cttctcccag
     3481 gtcttcggcg aggcgttcgg cgacgtcttc ggcggccgcg ccggaggcgg cgcccgccag
     3541 catggcccgc gccgcggctc ggacctgcgc tacgacctcg agatcacgct ggaacaggcc
     3601 tacaagggcg ccgacgtcga gatttcggtc cccaccaccg ccacctgcga cacctgcgag
     3661 ggcaccggcg ccaaggccgg caccaagccc agcacctgca ccacctgcca gggcgcgggc
     3721 cgcgtccgct ccgccaacgg cttcttccag gtcgaacgca cctgcccccg ctgcggcggc
     3781 cacggccaga tgatcgccga gccctgcgcc agctgcgccg gccacggtca ggtgcgcaag
     3841 aaccgcaagc tccagctcaa ggttccggcc ggggtcgacg acggctcgcg tatccgcctg
     3901 tcgggcgagg gcgacgccgg cacgcgcggc ggtccgcgcg gggacctcta tgtcttcctg
     3961 tccgtcacgc cgcacgaact gttcgagcgc gacaacctcg accttctggt caccgtcccc
     4021 gtgccaatga ccacggcggc cctgggcggc gagatcgatg cgccctgcct gacctccgaa
     4081 gcctgcgacg gccgctgccg cgcgccggtg accgtccccg cgggggccca gaccggcaag
     4141 acggtgcgca tcaagggcaa gggcatgccg catctgaacg gcaagaaccg cggcgatctg
     4201 gtcgtcgagc tgttcgtcga gacgcccacc gacctgaccc cgcaccagaa ggaactgctg
     4261 caggaactgg ccgcctcgct gggcgagagc cagaccccgc gcaactcgtc cttcgccggc
     4321 aaggccaaac gcttctgggc cgacatcctc gggtcggaag cgccgcagta ggttcaacaa
     4381 aaccacccgt caccctcggg cttgtcccga gggcccattc gtccgtcgtg ggatgggcgg
     4441 tgttgcaagc gcggatgaac acgaccgaac ctgcgccctg aaccatgggt cctcgggaca
     4501 agcccgagga tgacggacgg ggggattgtc gtcagtatgc tgcaaacc
//
LOCUS       DASVVN010000463         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_283115,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000463 DASVVN010000000
VERSION     DASVVN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283115"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1004
                     /locus_tag="VF686_07430"
     CDS             18..1004
                     /locus_tag="VF686_07430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX8661683.1"
                     /translation="MSSPRLKVVLTRRLPDAVETRARELFDAELNLTDRPMDRAALEA
                     AVQRADVLVPTITDVIDADLVSKAGDQLKMIANFGAGVDHIDIDAAVARGIIVTNTPG
                     VLTEDTADLAMALILAVSRRIVEGAQVVAAGGFEGWTPTWMTGRKLWGKRLGIVGMGR
                     IGQALARRAKAFGMQVHYHNRKPVPAMIAEELGATYWDDLDQMLARMDLISLNCPATK
                     ETHHLLSAERLALLRPEALLINTARGELIDEAALAQAVAKGRIGGVGLDVFENEPLVH
                     PGLLGHPNVVLLPHLGSATIEARQDMGDRVIANIQTFANGHRPPDRVIPAML"
     gene            1070..>1597
                     /locus_tag="VF686_07435"
     CDS             1070..>1597
                     /locus_tag="VF686_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX8661684.1"
                     /translation="MAGMNKAARAVLSRRGLLGGAGALAGAAALSPGMAWAQGGAYPL
                     SEFFKSTMSSGAALSPSGDRIAVAENLGSDEEPHSAVDFIDAANPEGQRRRIDLGPVR
                     VSFIDWASDDCLLASVILKGTTGTRQIAGSNRRAQGVDYYMRRTVSVNATTGAVAVLF
                     SDQHNRMRNTYDMGRI"
BASE COUNT          271 a          528 c          562 g          236 t
ORIGIN      
        1 gaattcgaag gccccgaatg tcctcccccc ggcttaaggt cgtattaacc agacgccttc
       61 cggacgcggt cgaaacccgc gcgcgcgagc tgttcgacgc cgagctgaac ctgacggaca
      121 ggcccatgga ccgggcggcg ctggaagcgg ccgtgcagag ggccgacgtg ctggtcccga
      181 ccatcaccga cgtcatcgac gccgaccttg tttcgaaagc cggcgaccag ctgaagatga
      241 tcgccaactt cggcgccggg gtggaccata tcgatatcga cgccgccgtg gcgcgcggga
      301 tcatcgtaac caacaccccg ggcgtcctga ccgaggacac cgccgacctg gccatggccc
      361 tgatcctggc cgtcagccgc cgcatcgtcg aaggcgccca ggttgtggcc gccggcgggt
      421 tcgagggctg gacaccgacg tggatgacgg ggcgcaagct gtggggcaag cggctgggca
      481 tcgtcggcat gggccggatc ggccaggcgc tggcgcggcg agccaaggcg ttcgggatgc
      541 aggtccacta tcacaatcgc aaacccgtcc cggccatgat cgccgaggag ctgggggcca
      601 cctactggga cgatctggac cagatgctgg cgcggatgga cctgatctcg ctgaactgcc
      661 cggcgacgaa agagacccac catctgctgt cggccgaacg gctggcgctg ctgcggcccg
      721 aggcgctgct gatcaacacc gcgcgcggcg agctgatcga cgaggccgcg ctggcgcagg
      781 ccgtggcaaa ggggcggatc ggcggggtcg gtctggacgt gttcgagaac gagccgctgg
      841 tccatccggg gctgctgggc caccccaatg tcgtcctgtt gccccacctc ggctcggcca
      901 cgatcgaggc gcgtcaggac atgggcgacc gggtcatcgc caatatccag acgttcgcga
      961 acggccaccg gccgccggac cgggtcattc ccgcgatgct gtgacacgtc gcgcttgacg
     1021 gcgcgtgaaa ccttgggttg gaagggtgtc gggcaggggg cgtatcgaaa tggctggaat
     1081 gaacaaggcg gcgcgcgccg ttctgtcgag gcgcggcctt ttgggcggcg ccggggcgct
     1141 ggcgggcgca gcggccctgt cgccgggcat ggcctgggcc cagggcggcg cctatccgct
     1201 gagcgaattc ttcaagtcga cgatgagcag cggcgcggct ctctcgccct ccggcgaccg
     1261 gatcgccgta gccgaaaatc tcggctccga cgaggaaccc cacagcgccg tcgacttcat
     1321 cgacgccgcc aaccccgaag gtcagcgtcg caggatcgat ctgggcccgg tccgggtcag
     1381 cttcatcgac tgggcgagcg acgattgcct tctggccagc gtgatcctca agggtacgac
     1441 ggggacccgg cagatcgccg gttcgaaccg gagagcgcag ggcgtcgact actacatgcg
     1501 ccgcaccgtc tcggtgaacg cgacgacggg cgctgtggcg gtgctgttca gcgatcagca
     1561 caaccgcatg cgcaacacct acgacatggg ccgcatc
//
LOCUS       DASVVN010000464         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_17413, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000464 DASVVN010000000
VERSION     DASVVN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17413"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VF686_07440"
     CDS             <1..1045
                     /locus_tag="VF686_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8661685.1"
                     /translation="GKTIEFVNMTVGKEFDRLVALIKDWKPDSVVHFAEQRAAPYSMK
                     SARHKLYTVDNNLNATNHLLAAIVESGLDVHLAHLGTMGVYGYTTAGLRIPEGYLKVT
                     VDTDFGPAEQEILFPPNPGSIYHMTKTQDALLFQFYAKNDGVRITDLHQGIVWGAQTE
                     ETRLDERLINRFDYDGDYGTVLNRFLMQAAVGYPLTVHGTGGQTRAFIHIQDTVRCVE
                     LALANPPKSGERVKILNQMTESRRVRDLASMIADMTGAEVHNVPNPRQEADENELVVA
                     NDQFRELGLKPITLAEGLMQDVTEIARRYADRADVSKIPCVSSWNSKRAKAVQTEPTL
                     TAPPKAPVHARSA"
     gene            1053..1937
                     /locus_tag="VF686_07445"
     CDS             1053..1937
                     /locus_tag="VF686_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661686.1"
                     /translation="MSNVPSSLLVFGGGWLGQAAAREAVRRGGRAVLTSRDVETRAAL
                     IAEGFAAIDPADAAALTAAVAEASSILVTAPPEADGCPGLKALAPAITGSASWPDWIG
                     YVSSTAVYGDRAGGWALEDSELNAASLEGARRVRAERDWLDAGKGMGLTVQLFRLPAL
                     YGPGRSPLDRLRDGTARLVRKPGQVFNRIHVEDAVSGLFASMARPRPGAAYILCDDEP
                     APADVVMEGAAGRLGLPLPPEVDWTDPSVSDAMRRFYLDSKRLSNARAKAELGWRPRY
                     PGWRDGLEAMIRDAAEQA"
     gene            complement(2036..>2303)
                     /locus_tag="VF686_07450"
     CDS             complement(2036..>2303)
                     /locus_tag="VF686_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX8661687.1"
                     /translation="WQGISIGWDVGGNASRVFTLVYGLFHPDMIYRRYPGVEGSAYLV
                     GGLGVNYQRADGIVLAPIRTGVGLRLGANVGTMAYSRQRNLLPF"
BASE COUNT          385 a          794 c          788 g          336 t
ORIGIN      
        1 cggcaagacc atcgagttcg tcaatatgac ggtgggcaag gagttcgacc gtctcgtcgc
       61 cctgatcaag gactggaagc ccgacagcgt cgtccatttc gccgaacagc gggccgcgcc
      121 ctattcgatg aagtcggccc ggcataaatt gtacacggtc gacaacaatc tgaacgccac
      181 caaccacctg ctggccgcca ttgtcgaaag cgggctggac gtgcatctgg cgcacctcgg
      241 caccatgggc gtctatggct acaccaccgc cggcctgcgc atccccgagg gctatctgaa
      301 ggtcacggtc gataccgact tcggcccggc cgagcaggag atcctgttcc cgccgaaccc
      361 gggctcgatc taccacatga ccaagaccca ggacgccctg ctgttccagt tctacgccaa
      421 gaacgacggg gtccggatca cggacctgca ccagggcatc gtctggggcg cccagactga
      481 ggaaacgcgt ctggatgagc ggctgatcaa ccggttcgac tatgacggcg actacggcac
      541 ggtgctgaac cgcttcctga tgcaggcggc cgtcggctat ccgctgaccg tgcacggcac
      601 gggcggccag acccgggcct tcattcacat ccaggacacg gtgcgctgcg tcgaactggc
      661 cttggccaat ccgccgaagt ccggtgagcg ggtcaagatc ctgaaccaga tgaccgagag
      721 ccgccgggtg cgcgacctgg cgtccatgat cgccgacatg accggggccg aggtccacaa
      781 cgttcccaat ccgcgacagg aagccgacga gaacgagctg gtggtcgcca acgaccagtt
      841 ccgcgagttg ggcctcaagc cgatcaccct cgcggagggt ctgatgcagg acgtgaccga
      901 gatcgcgcgc cgctacgccg accgggccga cgtgagcaaa atcccctgcg tgtccagctg
      961 gaacagcaaa cgagcgaagg ccgtgcagac cgagcccacc ctgaccgccc cgcccaaggc
     1021 gccggttcac gcccggtcgg cctgaccctt tcgtgtcgaa cgtcccttca tccctgctgg
     1081 tcttcggcgg cggctggctg ggtcaggcgg cggcgcgtga ggcggtgcgg cgcggcggcc
     1141 gggcggttct gaccagccgc gacgtcgaaa cccgcgccgc cctgatcgcg gaaggcttcg
     1201 ccgccatcga tcccgccgac gccgcggcgc tgaccgcggc ggtcgccgaa gcatcgtcca
     1261 ttcttgtcac cgctccgccc gaggccgacg gctgcccggg cctgaaggca ttggcgcccg
     1321 cgatcaccgg gtccgcgtcg tggcccgact ggatcggcta cgtctcctcc acggccgtct
     1381 atggcgaccg cgccgggggc tgggcgctgg aggacagcga gctgaacgcc gccagtctgg
     1441 aaggcgcgcg gcgggtgcgg gccgaacgcg actggctgga cgccggaaag ggcatgggcc
     1501 tgacggtgca gctgttccgc ctgcccgccc tgtatggccc cggccgctcg ccgctggacc
     1561 gtctgcgcga cggcacggcc cggctggtga gaaagccggg gcaggtgttc aaccgcatcc
     1621 atgtcgagga cgccgtctcg ggcctgttcg cctcgatggc gcggccgcgg cccggcgcgg
     1681 cctatattct gtgcgacgac gaaccggccc ccgccgacgt ggtgatggag ggcgcggccg
     1741 ggcggctggg cctgcccctg ccgcccgagg tggactggac cgatccgtcg gtatccgacg
     1801 ccatgcgccg cttctatctg gactcgaaac gcctctcgaa cgcgcgggcc aaggccgaac
     1861 tcggctggcg gccgcgctat ccgggctggc gcgacgggct ggaagcgatg atccgcgacg
     1921 ccgccgagca ggcgtgagga tgcgagacat ttcgggcgat acatccgcga catcgcgccg
     1981 accgtagtaa ccgggacatc cgcgacaggc gcgacacgcg gcggttcctc ccggcctaga
     2041 agggcagcag gttgcgctgg cggctgtagg ccatggtgcc gacattggcg ccgaggcgca
     2101 ggccgacgcc ggtgcggatc ggggccagga cgatgccgtc ggcgcgctgg tagttcacgc
     2161 cgaggccgcc gaccagatag gccgagccct ctaccccggg atagcggcgg tagatcatgt
     2221 ccgggtggaa caggccgtag accaaggtga agaccctgct ggcgttgccg ccgacgtccc
     2281 agccgatcga aatgccttgc cag
//
LOCUS       DASVVN010000465         7130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_388083,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000465 DASVVN010000000
VERSION     DASVVN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388083"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VF686_07455"
     CDS             <1..368
                     /locus_tag="VF686_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar assembly protein FlbE"
                     /protein_id="HEX8661688.1"
                     /translation="VVAASALDRFPAAPLQAALEALGQEVDSSPRLVIRTGDLDDATH
                     ARIQQLCADAGFSGVVAFRNEPMMPAAAFQLEWADGRAAFDPAEAFTRIGEALNSALA
                     AEAGHAEPMTPETSLEGKP"
     gene            368..700
                     /gene="fliN"
                     /locus_tag="VF686_07460"
     CDS             368..700
                     /gene="fliN"
                     /locus_tag="VF686_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX8661689.1"
                     /translation="MASDTMSLEEFPDSTAVTIADEAIDKTASDLAPVFDVPVNISAV
                     LGKAHMSVAQLLKLNRGSVLQLDRKVGEAIDIFVNNRLVARGEVVVVEDRLGVTMTEI
                     IKTEDSNA"
     gene            738..2132
                     /locus_tag="VF686_07465"
     CDS             738..2132
                     /locus_tag="VF686_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8661690.1"
                     /translation="MRLLVVGRLSGQLATAVKMAMAHGAKVSHVERGDQATEQLRRGQ
                     GADLLMVDYRVDIAALIAANENERIHVPVVACGVDADATDAANAIRAGAKEFIPLPPE
                     ADLIAAVLSAVADDERPLISADPSMKAVIQLADQVARSEASILITGESGVGKEVMARY
                     LHTGSKRADRPFISVNCAAIPDNLLESELFGHEKGAFTGAMARRIGKFEEADGGTLLL
                     DEISEMDVRLQAKLLRAIQERVIDRVGGTKPVPVNIRIIATSNRDLAKSVAEGAFRED
                     LLYRLNVVNLRLPALRERPGDIAVLADHFVKKYAAANGVPVRPISADAKRALVSHRWN
                     GNVRELENAMHRAVLLAVGPEIDVEAIRLPDGQPLAMGSHGAAPDVGVAGRAAHVADI
                     VTRAHVGQTVAQMEKTLILDTLSHCLGNRTHAATILGISIRTLRNKLNEYADEGTSIP
                     APQSGIAATGYAAA"
     gene            2135..3040
                     /gene="bla"
                     /locus_tag="VF686_07470"
     CDS             2135..3040
                     /gene="bla"
                     /locus_tag="VF686_07470"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX8661691.1"
                     /translation="MTFGTDRRSVLTGLGALGVMGCVRASETNARQAAPAAPEAFDLS
                     VLERDNGGRLGFVAHDMGSGRRLAWRGDERFVYCSTFKMYLAAATFLRVQAGQERLDR
                     MIPVSAADMVNHAPVTEPAIGRSLSIEALMKGTVEVSDNPAANLLLKAMGGIEPMQAF
                     YRSLGDDSTTANRFEPEMNRLDGDKDTITPLQSATNIKRLLVDAATPLSDLHKALLLR
                     WMTDTPTGMARIKAGVPAGWTVAHKTGTGGYGPTNDIGVLYPPAGAPVIVAAYYHAAA
                     AGPAARHEAVIAAATRQALLAFGRA"
     gene            3061..5154
                     /gene="flhA"
                     /locus_tag="VF686_07475"
     CDS             3061..5154
                     /gene="flhA"
                     /locus_tag="VF686_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX8661692.1"
                     /translation="MMKDGYARPTGRDMLGWIARGEVAMAIGVIGVILLLILPIPTFL
                     LDILLALSLVSSVLILMTAVMMKKPLDFAIFPTVLLVSTLFRLGLNLASTRLVLSHGH
                     EGSGGAGQVIQAFGSLMMGGSFIIGIIIFAIILVVNFVVITKGSTRIAEVSARFTLDS
                     MPGKQMAIDADLSSGLIDEETAKRRRKELEQESTFFGAMDGASKFVRGDAVAGLIIVF
                     INAIGGILIGTIQHGMPIGEAANSYVALTIGDGLVTQVPAIVISIAAGFLVSKAGVEG
                     TADKALVAQLATNPVSLGVVAAASGLIGLIPGMPLLPFAALALGTGFMAWKLGRERLK
                     PKPTEAEMAATASGPREEVEEPIGTALTIDEVKIELGYSLLSLINDLEGRRLTDQVRA
                     LRRSLAQEYGFVMPSVRILDNMRLGTQGYAIRIKEMEAGVGEVRLGSLMAMDPAGRQV
                     ELPGEHVKEPAFGLPATWIDESLREEATFRGYTIVDPSTVLTTHLTEILKENMADLLS
                     YAEVQKLLKELGTEEKKLVDELIPSVVTVTTLQRVLQTLLREKVSIRDLAAILEGLAE
                     AAPHTSSVTTLVEHVRGRLARQLCWQHKGDDGALPIVTLSPEWEAAFADALVGQGEDR
                     QLAMAPSRLQDFIRSVRDVFERAAMAGETAVLLTGPQIRPYVRSIIERFRGQTVVMSQ
                     NEVHPRARLRTIGTV"
     gene            5206..5760
                     /locus_tag="VF686_07480"
     CDS             5206..5760
                     /locus_tag="VF686_07480"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8661693.1"
                     /translation="MRLLVLVAALTVLTGGPAAAQTAGQLVDTSSVEANGHRIIQLSL
                     KISAPPSEVWSALTTSEGWRRLGVSFAEVDFRTGGMIETGYAPGSVAGAQGNISNQIV
                     AYVPERMIAIRNVKAPPGFPHAAEFAETATVMELDPIGDGHTRVTLTATGFAPGPAYD
                     TLYGFFHQGNGLTLETLRDSFAAD"
     gene            complement(5762..6472)
                     /locus_tag="VF686_07485"
     CDS             complement(5762..6472)
                     /locus_tag="VF686_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661694.1"
                     /translation="MVWWWWIVPAVVGLLGVILFIKGVTGLFKGRFFSGLLVGAGGAG
                     FMAAAVIASLLALDIQTYGRLTYERPVATIQTRQLGPQYFEATVIQPDRGEHAPAVTN
                     LYPLHGDEWRIEAQVLKWKPWANVLGLDTQYRLDRLSGRYASIEQEISAERSVHPLSG
                     GDTSVTWLPWKVSTWDTARRYRRYVDAVDTLYGGGAYMPMADGAKYEVWITQSGLVAR
                     PANDAARTASSGGWTEVR"
     gene            6587..>7130
                     /locus_tag="VF686_07490"
     CDS             6587..>7130
                     /locus_tag="VF686_07490"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX8661695.1"
                     /translation="MIPALRPTLQALGHFLRTPSLSRSVVALLTGAIALLLIVNVGVF
                     VMLQRTSDFNHNVDRGHRIRLAATEVMTLSVDAETGQRGFLLTGRAEYLEPYTSSVAR
                     MPEHLDELARLTAGDPDLSPRAERIRELSQQRLDVIDRTIALGRMGRGEDAVQLIRDG
                     SGKVLMDQMRAETSAINEIEA"
BASE COUNT         1171 a         2579 c         2267 g         1113 t
ORIGIN      
        1 gtgtggtcgc cgcctccgcc ctggaccgct tccccgctgc gccgcttcaa gccgcgctgg
       61 aagccttggg ccaggaggtc gattcctcgc cgcgcctcgt gatccgcaca ggcgatctgg
      121 acgatgcgac ccacgcccgc atccaacagc tgtgcgccga cgccggcttc tccggcgtgg
      181 tcgccttccg caacgagccc atgatgcccg ccgcggcctt ccagctggaa tgggccgacg
      241 gccgcgccgc cttcgacccc gccgaagcct tcacccgcat cggcgaagcc ctcaactccg
      301 ccctggccgc cgaagccggc cacgcagaac ccatgacccc tgaaacctct ctcgaaggga
      361 agccgtaatg gcgtccgaca ccatgtccct ggaggagttc ccggactcca ccgccgtgac
      421 catcgccgac gaggcgatcg acaaaaccgc cagcgatctg gcgcccgtct tcgacgtgcc
      481 ggtcaacatc tcggccgttc tgggcaaggc gcatatgtcg gtcgcccagc tgctgaagct
      541 gaaccgcggc tcggtgcttc agctggaccg caaggtcggc gaggccatcg acatcttcgt
      601 caacaaccgg ctggtcgccc gcggcgaggt cgtcgtcgtc gaggaccggc tgggcgtgac
      661 catgacggaa atcatcaaga cggaagacag caacgcctga ttggcggcgc tctggtagag
      721 attgaaggag aagacccatg cggcttctgg tggttggcag gctcagcgga cagctggcga
      781 cggcggtgaa gatggccatg gcgcacggcg ccaaggtcag ccacgtcgag cgcggcgatc
      841 aggcgacgga acagctgcgc cgggggcagg gcgccgacct gctgatggtc gactaccgcg
      901 tcgatatcgc cgccctgatc gcggccaatg agaacgagcg catccatgtg ccggtggtgg
      961 cctgcggggt cgacgccgac gccacggacg ccgccaacgc catccgggcc ggggccaagg
     1021 agttcatccc gcttccgccg gaagccgacc tgatcgccgc cgttctgtcg gccgtcgccg
     1081 acgacgagcg tccgctgatc tcggccgatc cctcgatgaa ggcggtgatc cagctggccg
     1141 accaggtcgc ccgctcggaa gcctcgatcc tgatcaccgg cgaaagcggc gtcggcaagg
     1201 aggtgatggc ccgttatctc cacaccggct ccaagcgcgc cgaccgtccc ttcatcagcg
     1261 tcaactgcgc cgccattccc gacaacctgc tggagtcgga gctgttcggg cacgagaagg
     1321 gcgccttcac cggcgccatg gcccgccgca tcggcaagtt cgaagaggcc gacggtggca
     1381 ccctgctgct cgacgaaatc tccgagatgg acgtgcgcct gcaggccaag ctgctgcgcg
     1441 cgattcagga gcgggtcatc gaccgcgtcg gcgggaccaa gccggtgccg gtcaacatcc
     1501 gcatcatcgc cacctccaac cgggatctgg ccaagtcggt ggccgagggc gccttccgcg
     1561 aagacctgct gtatcgcctg aacgtcgtga acctgcgcct gcccgccctg cgcgagcgtc
     1621 cgggcgacat cgccgtgctt gccgatcatt tcgtcaagaa atacgccgcc gccaatggcg
     1681 tgccggtccg gccgatctcg gccgatgcga agcgcgccct ggtctcgcac cgctggaacg
     1741 gcaacgtccg ggagctggaa aacgccatgc accgcgcggt gctgctggcg gtcggcccgg
     1801 agatcgatgt cgaggccatc cgcctgccgg acggccagcc gctggcgatg ggcagccacg
     1861 gcgccgcccc tgacgtcggt gtcgccggcc gcgccgccca tgtcgccgac atcgtcaccc
     1921 gcgcccacgt cggccagacg gtggcgcaga tggaaaagac cctgatcctc gacaccctgt
     1981 cgcactgcct cggcaaccgc acccatgcgg ccaccattct cggcatctcg atccggaccc
     2041 tgcgcaacaa gctgaacgaa tacgccgacg aggggaccag catcccggcc ccgcaatcgg
     2101 gcatcgccgc caccggctac gcggccgcct gatcatgacc ttcggaacgg accgcagaag
     2161 cgtcctcacc ggccttggcg cgctgggcgt catgggctgc gtacgcgcat ccgaaacaaa
     2221 cgcgcgtcaa gccgccccgg cggctccgga agccttcgac ctctccgtgc tggagcggga
     2281 caacggcggc cggctgggct tcgtcgccca cgatatgggc tcgggtcgca gactggcttg
     2341 gcggggcgac gaacggttcg tctattgctc gacgttcaaa atgtacctcg ccgccgcgac
     2401 cttcctgcgc gtgcaggccg gacaggagcg gctggaccgc atgattccgg tgtcggccgc
     2461 cgacatggtc aaccatgcgc cggtgacgga gccagcgatc ggccggtcgc tcagcatcga
     2521 ggccttgatg aagggcacgg tcgaggtcag cgacaacccc gccgccaacc tgttgctcaa
     2581 ggccatgggc ggcatcgagc ccatgcaggc cttctaccgc tcgctcggcg acgacagcac
     2641 cacggcgaac cgtttcgagc ccgagatgaa ccgcctcgac ggcgacaagg acaccatcac
     2701 gcccctgcag tccgcgacca acatcaagcg cttgctggtc gacgccgcca cgcccctgtc
     2761 ggatctgcac aaggcgctgc tgctgcgctg gatgaccgac acccccaccg gcatggcccg
     2821 gatcaaggca ggcgttcccg cgggctggac cgtcgcgcac aagaccggaa ccggcggcta
     2881 cggcccgacc aacgacatcg gcgttctcta tccgccggcc ggggcgcccg tgatcgtcgc
     2941 cgcctactat cacgccgctg ccgccgggcc cgcggcgcgc catgaggccg tcatcgctgc
     3001 tgcgacccga caggccctgc tcgccttcgg tcgcgcatga ccgacattcc cgcctcgccg
     3061 atgatgaagg acggctacgc ccgtccgacg ggccgcgaca tgctaggctg gatcgcgcgc
     3121 ggcgaggtgg ccatggccat cggcgtcatc ggcgtcatcc tgctgctgat cctgccgatc
     3181 ccgaccttcc tgctggacat cctgctggcg ctgtcgctgg tctccagcgt gctgatcctg
     3241 atgactgcgg tcatgatgaa gaagccgctc gacttcgcca tcttcccgac cgttcttctg
     3301 gtgagcaccc tgttccggct cggcctgaac ctggcctcga cgcgtctggt gctcagccac
     3361 ggacacgagg gctccggcgg cgccggtcag gtgatccagg ccttcggcag cctgatgatg
     3421 ggcggcagct tcatcatcgg catcatcatc ttcgccatca ttctggtggt gaatttcgtg
     3481 gttatcacca agggctcgac ccgcatcgcc gaggtctcgg cccgcttcac cctggactcc
     3541 atgccaggca agcagatggc catcgacgcc gacctgtcgt ccggcctgat cgacgaggaa
     3601 accgccaaac gccgccgcaa ggaactggag caggaatcga ccttcttcgg cgccatggac
     3661 ggcgcctcga aattcgtgcg cggcgacgcc gtcgctggcc tgatcatcgt cttcatcaac
     3721 gccatcggcg gcatactgat cggcactatc cagcacggca tgccgatcgg cgaggccgcc
     3781 aacagctatg tcgccctgac catcggcgac ggtctggtga cccaggttcc cgccatcgtc
     3841 atctccatcg ccgccggctt cctcgtgtcc aaggcgggcg tcgagggcac ggccgacaag
     3901 gcgctggtcg cccagctggc caccaacccc gtcagcctcg gcgtcgtcgc ggccgcttcg
     3961 ggcctgatcg gcctgatccc cggcatgccg ctgctcccgt tcgccgccct ggccctcggc
     4021 acgggcttca tggcatggaa gctgggccgc gaacggctga agcccaagcc caccgaggcc
     4081 gaaatggccg ccaccgcctc cggcccgcgc gaagaggtcg aggagcccat cggcaccgcc
     4141 ctgaccatcg atgaggtcaa gatcgagctg ggctattccc tgctgtccct gatcaacgat
     4201 ctggagggcc gtcgcctgac cgatcaggtc cgcgccctgc gccgttcgct ggcgcaggag
     4261 tacggcttcg tcatgccgtc ggtccgcatc ctcgacaaca tgcgcctcgg cacccagggc
     4321 tacgccatcc gcatcaagga gatggaggcc ggcgtcggcg aggtccggct tggctccctg
     4381 atggccatgg acccggccgg ccgccaggtc gagctgccgg gcgagcacgt caaggagccc
     4441 gccttcggcc tgcccgccac atggatcgac gaaagcctgc gcgaggaggc caccttccgc
     4501 ggctacacca tcgtcgatcc gtcgacggtg ctgaccaccc atctgaccga gatcctcaag
     4561 gagaacatgg ccgacctgct ctcctatgcc gaggtgcaga agctgctgaa ggaactgggg
     4621 accgaagaga agaagctggt cgacgaactg atcccctcgg tcgtcaccgt caccaccctc
     4681 cagcgcgtcc tgcagaccct gctgcgcgag aaggtctcga tccgcgatct ggccgccatt
     4741 ctggaaggtc tggccgaggc cgcgccgcac accagctcgg tgacgacgct ggtcgaacac
     4801 gtccgtggcc gtctggcccg ccagctgtgc tggcagcaca agggcgacga cggcgccctg
     4861 cccatcgtca ccctgtcgcc ggaatgggag gccgccttcg ccgacgccct ggtcggtcag
     4921 ggcgaggacc gccagctggc catggccccg tcacggctcc aggacttcat ccgctccgtc
     4981 cgcgacgtct ttgaacgcgc cgccatggcc ggtgagacgg cggtcctgct gaccggcccg
     5041 cagatccgcc cctacgtccg ctcgatcatc gaacgcttcc gcggccagac ggtggtgatg
     5101 agccagaacg aggtgcatcc gcgcgcccgg ctgaggacca tcgggacggt ctagggaagc
     5161 cggcggcctg acgttataag ggtgcgcaac tcaaggagag ttccgatgcg cctcttggtc
     5221 cttgtcgccg ccctgaccgt gctgaccggc ggccccgcgg cggcccagac ggccggccag
     5281 ttggttgaca cgtccagcgt cgaggcgaac ggccaccgga tcatccagct gtccctcaag
     5341 atctccgcgc ccccgtccga ggtctggtcc gccctgacca cgtcggaggg ctggcgtcgc
     5401 ctgggcgtca gcttcgccga ggtcgatttc cggacgggcg gtatgatcga gacgggctac
     5461 gcccccggtt ccgtggcggg cgcgcagggc aacatcagca accagatcgt cgcctatgtg
     5521 cccgagcgca tgatcgccat ccgcaacgtg aaagccccgc ccggttttcc ccatgcggcc
     5581 gagttcgcgg agaccgccac ggtcatggag ctcgacccga tcggcgacgg ccatacccgg
     5641 gtgaccctga cggccaccgg cttcgcgccg gggccggcct atgacacgct gtacggcttc
     5701 ttccaccagg gaaacggcct gacgctggaa actctgcgcg acagcttcgc ggcggactga
     5761 cctagcgaac ctcggtccag ccgcccgatg acgccgtccg ggccgcgtcg ttcgccggac
     5821 gcgccaccag acccgactgg gtgatccaga cctcgtattt cgccccgtcg gccatcggca
     5881 tataggcgcc gcctccgtac agggtgtcga ccgcgtcgac atagcgccgg tagcgccgtg
     5941 ccgtgtccca ggtgctgacc ttccacggca gccaggtgac cgacgtgtcg ccgcccgaca
     6001 gcggatgaac gctgcgctcg gcgctgatct cctgctcgat cgaggcgtag cggcctgaca
     6061 gccggtccag ccggtattgc gtgtccagtc ccagcacatt ggcccacggc ttccatttca
     6121 gaacctgggc ctcgatgcgc cactcgtcgc cgtgcagcgg atacagattg gtcaccgccg
     6181 gcgcgtgctc gccccggtcc ggctggatca ccgtcgcctc gaaatactgg gggcccagct
     6241 ggcgggtctg gatggtggcc accggccgtt cataggtcag ccggccatag gtctggatgt
     6301 ccagggccag cagggaggcg atcaccgccg ccgccatgaa gcccgcgccg ccggcgccga
     6361 ccagcaggcc gctgaagaac cggcccttga aaaggcccgt cacgcccttg atgaacagga
     6421 tgacgccgag caggccgacg accgccggaa cgatccacca ccaccagacc atgtcaatct
     6481 cccccgatcc tgacggacca cctttacctt aaatcgcgag agcggggagg gtttctcccg
     6541 ggacgcgact ggacagggcc gaaggcgccg ctaatgctcc cttccgatga taccggcgct
     6601 gcgtcccacg cttcaggccc tcggccattt cctgcgcacg ccgtcgctca gccggtccgt
     6661 ggtcgccttg ctgacggggg ccatcgccct gctgctgatc gtcaatgtcg gcgtcttcgt
     6721 gatgctgcag cggaccagcg acttcaatca caacgtcgat cgcggtcacc gcatccgtct
     6781 cgccgcgacc gaggtgatga ccctgtcggt cgacgccgag accggtcagc gcgggtttct
     6841 gctgacgggc cgcgccgagt atctggaacc ctatacgtcc agcgtcgccc gcatgcccga
     6901 gcatctggat gaactggcgc ggctcacggc gggggacccc gacctgtcgc cccgcgccga
     6961 acgtattcgc gaactgtccc agcagcggct cgacgtcatc gaccgcacca tcgctctggg
     7021 gcgaatgggc cgcggggagg atgcggtcca gcttatccgc gacggctccg gcaaggtcct
     7081 gatggaccag atgcgggccg agacctcggc catcaacgag atagaggcga
//
LOCUS       DASVVN010000466         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_412703,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000466 DASVVN010000000
VERSION     DASVVN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412703"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF686_07495"
     CDS             complement(<1..296)
                     /locus_tag="VF686_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661696.1"
                     /translation="MKLSLITVAVAGALALAACSGQDDAAKTDAAAADAAAADAAAMA
                     PADGAMAPADGSMAGSTTGTTGTTGTTGTTSGTGSTTGQTPGTLPDDTGTTSPTT"
     gene            complement(446..1288)
                     /locus_tag="VF686_07500"
     CDS             complement(446..1288)
                     /locus_tag="VF686_07500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8661697.1"
                     /translation="MAKRLKTALIYDFDGTLARGNMQEVSFIPSIGMTPKQFWDSVYP
                     ETQKADGDGILMYMQMMLAAAQRTGQPVTRQALRDHGKEVALFEGLRDMSWFDRMDAF
                     GAQFGLEIEHYIISAGLTEMIEGCPIAKAFKHVYASKYVYDADDVAIWPAVGVNYTTK
                     TQYLFRINKGVFNHWDDERINRFIPDDERPIPFERMIFLGDGDTDVPTMKMMHTKGGF
                     SIAVYDPETTPRDTTKIHRLISEDRVNFVAAADYREGSPVDLIVKGLIGRIAVNAGRM
                     PADG"
     gene            complement(1326..>3559)
                     /locus_tag="VF686_07505"
     CDS             complement(1326..>3559)
                     /locus_tag="VF686_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8661698.1"
                     /translation="LAVRHRLAEADLVRARADLADCDGALAAFEDVRLALAPDGRTRR
                     LGAPGAWDAVVRDLAPQGEEDGDPSLIVLDAVREAAGPRLDALIDQGEAFDAVLEGAA
                     GAWSVEGRSSAGAAWLKVSRLGLVGTATESGLALLADSYPAPTWITDTAGRLAWANKA
                     WLTEMKADNVEAAREKGLSFDRGADAIVAEAGRTHQRQDGFRWTTGGGRRRAWRIVAE
                     PVQGGAGAVLAFALDVTEAEETRDTLRRHVEAHDETLNHLADAVAIFGPSKRLAFHNT
                     AFQSLFSIDPAWLDERPTHAELLDKLRQRRLLPEVIDYAGWKANELDFYGASEAAPDD
                     SWSLPDGRTLRVVRQPHPLGGVLLLFSDITDELKLRSRFNAQIQVQTATLDKLNDAVA
                     VFGSDGRIRLHNEAFDKFWSVTGDALDAAGDFDAVAALCQSTMPDPALWLGLKARVAD
                     PDPESRVPIAGEGRTADGRIASWLTRPLPDGATLVAFSDVTAQRGLEQALAAKAQALE
                     ESTALKREFVGSVSYELRTPLTTIVGYSELLEMQADLPERSRQHAGAIRVAANQLAGS
                     IDDVLDMAQIDAGEMELTLGDVRVCDLLDDAAEKSRARIEGRGASLTVTCPASLRPIR
                     ADAQRVGQALEHLLDNAARSVSEGGAVTLTAEANTSEVRIRVEDSGRGIPYHLQAHVF
                     DRFVRRERGGPGVGLALVKALVELHGGWAEVESEPGKGAAFILHLPLEAAGVAAAPEL
                     RLG"
BASE COUNT          519 a         1291 c         1162 g          587 t
ORIGIN      
        1 gtggtcggcg aggtcgtccc ggtgtcgtcg ggcagggttc ccggggtctg gccggtggtc
       61 gagccggttc ccgaggtggt tccggtcgtg cccgtggttc cggtcgtgcc ggtcgtcgag
      121 ccagccatcg atccatcggc gggcgccatg gcgccgtcag ccggagccat ggccgcggcg
      181 tcagcagcag cggcgtcagc agcggcggcg tcagtcttgg ctgcgtcgtc ctggccggag
      241 caggccgcaa gggccagggc gccggcgacg gcgacagtga taagcgagag tttcatgtgg
      301 gttcctccgg tcgaccccct ctgcgaattt ccgcaggttt cacggtcgcc atctcgctaa
      361 tcgcgtcacg ccgcgacccg ttcccgaatt tgtgagaacc ggtgaaaaaa cgttacgtat
      421 ccgtgtctta tgtctagaac cggacttagc cgtcggcagg catgcgcccc gcattcaccg
      481 cgatccgacc gatcagcccc ttgacgatca ggtcgaccgg cgagccctcg cgatagtcgg
      541 cggcggcaac gaaattgacc cggtcctccg agatcaggcg gtggatcttg gtcgtgtcgc
      601 gcggcgtcgt ctcgggatca tagaccgcga tcgagaagcc gcccttggtg tgcatcatct
      661 tcatggtcgg cacatcggtg tccccgtcgc cgaggaagat catccgctcg aacgggatcg
      721 gccgctcgtc gtccggaatg aaccggttga tgcgttcgtc gtcccagtga ttgaacacgc
      781 ccttgttgat gcggaagagg tactgggtct tggtcgtgta gttgacgccc accgccggcc
      841 agatggccac gtcgtcggcg tcatagacat atttcgaggc gtagacgtgc ttgaacgcct
      901 tcgcgatcgg gcagccctcg atcatctccg tcagcccggc cgagatgatg tagtgttcga
      961 tctccagccc gaactgcgcc ccgaaggcgt ccatccggtc gaaccagctc atgtcgcgca
     1021 gaccctcgaa cagggccact tccttgccgt gatcgcgcag cgcctgacgc gtcaccggct
     1081 gaccggtccg ctgggccgcg gccagcatca tctgcatata catcaggatg ccgtcgccat
     1141 cggccttctg ggtctcggga tagacgctgt cccagaactg tttcggcgtc atcccgatcg
     1201 acggaatgaa gctgacctcc tgcatgttgc cgcgcgccag cgtgccgtcg aagtcgtaga
     1261 tcagtgcggt tttcaagcgt ttggccatcg gcgaaatcct gcgttctccg ccgctctaaa
     1321 ccaatctagc cgagccgcaa ctccggggcc gcggccacgc ccgccgcttc cagcggcagg
     1381 tgcaggatga aggcggcgcc cttgccgggc tcgctctcga cctcggccca tccgccgtgc
     1441 agctcgacca gcgccttgac cagcgccagt ccaacgcccg gcccgccgcg ctcgcgccgc
     1501 acgaaacgat cgaagacgtg ggcctgcaga tggtagggaa tgccacgtcc gctgtcctcg
     1561 acgcggatgc gcacctcgga cgtattggct tcggcggtca gggtcacggc cccgccctcg
     1621 gacaccgagc gcgccgcatt gtccagcaga tgctccagcg cctggccgac cctctgggcg
     1681 tcggcgcgga tgggccgcag actggccgga caggtcacgg tcaggctggc gccgcgcccc
     1741 tcgatccggg ctcgcgactt ctccgccgcg tcgtccagca ggtcgcagac ccgcacgtcg
     1801 cccagcgtca gctccatctc gcccgcgtcg atctgggcca tgtccagaac atcgtcgatc
     1861 gagccggcca actggttggc ggcgacacgg atggcgcccg cgtgctgacg actgcgctcg
     1921 ggcaggtcgg cctgcatctc cagcagttcg gaatagccga cgatggtcgt cagcggcgtg
     1981 cgcagttcat aggagacgct gcccacgaac tcgcgtttca gcgccgtgga ttcctccagc
     2041 gcctgggcct tggcggccag cgcctgctcc agcccgcgct gcgccgtcac gtccgagaac
     2101 gccaccaggg tcgcgccgtc cggcagcggc cgcgtcagcc acgaggcgat ccgcccgtcg
     2161 gccgtgcggc cctcgccggc gatcggcacc cggctttcgg gatcgggatc ggccacccgc
     2221 gccttcagtc ccagccacag ggccggatcg ggcatggtcg actggcacag ggcggcgacg
     2281 gcgtcgaaat ccccggccgc gtccagcgcg tcgcccgtca cgctccagaa cttgtcgaag
     2341 gcctcgttgt gcaggcggat gcgcccgtcg gatccgaaca cggccacggc gtcgttcagc
     2401 ttgtccagcg tcgcggtctg cacctggatc tgggcgttga accggctgcg cagcttcagc
     2461 tcgtcggtga tgtccgagaa cagcagcagc accccgccca gcgggtgcgg ctggcgcacc
     2521 acccgcagcg tccgtccgtc cggcagggac cagctgtcgt cgggcgccgc ctcggacgcg
     2581 ccgtagaagt ccagctcatt ggccttccat ccggcgtagt cgatgacttc gggcagcagc
     2641 cgacgctggc gcagcttgtc cagcagttcg gcgtgggtcg gccgctcgtc cagccaagcc
     2701 gggtcgatgc tgaacagcga ctggaaggcg gtgttgtgga aggccagccg cttggatggg
     2761 ccgaagatgg cgaccgcgtc ggccagatgg ttcagcgtct cgtcatgggc ctcgacgtgg
     2821 cggcgcagag tgtcgcgcgt ctcttccgcc tcggtcacgt ccaaggcgaa ggccagcacg
     2881 gcgcccgccc cgccctgcac cggctcggcc acgatccgcc acgcccggcg acgaccaccg
     2941 cccgtggtcc agcggaagcc gtcctgacgc tgatgcgtcc gtcccgcctc cgcgacaatg
     3001 gcgtccgcgc cgcgatcgaa gctcagcccc ttctcgcgcg ccgcctcgac gttgtcggcc
     3061 ttcatctcgg tcagccaggc cttgttggcc caggccagcc ggcctgccgt atcggtgatc
     3121 catgtcgggg cgggatagga atcggccagc agggccagcc cactctcggt cgccgtgccc
     3181 accagtccca gtcgtgagac cttcagccat gccgcccccg ccgaggaccg gccctccacc
     3241 gaccaggccc cggccgcccc ttccagcaca gcgtcgaagg cctcgccctg gtcgatcagg
     3301 gcgtccagcc gcggccccgc cgcttcgcgc accgcgtcca gcacgatcag cgacggatcg
     3361 ccgtcctcct cgccctgcgg cgccagatcg cgaaccaccg cgtcccaggc gcccggcgcg
     3421 cccagccgcc gcgtccgtcc gtcgggcgcc agcgccagtc tgacgtcttc gaacgcggcc
     3481 agagccccgt cgcaatcggc cagatcggcg cgggcgcgga ccagatcggc ctcggccagc
     3541 cggtggcgca ccgccagcc
//
LOCUS       DASVVN010000467         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_412852,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000467 DASVVN010000000
VERSION     DASVVN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412852"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF686_07510"
     CDS             complement(<1..516)
                     /locus_tag="VF686_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX8661699.1"
                     /translation="MSIDRRLMLLGLAGLGAAVGGCGGGGGMLGDGGFAGRPSQADLR
                     DGNHVRNVGRTGFPEVSFADWSDAEPEYVLYPGDEIEIATPTAVELTRTQKVGPDGRI
                     SLPLVGQIMAGDRTIAELEADASAAYATQLRRPVVEVTLKTAGPIRVWVDGEVRTPGV
                     YEMTGDLDAYQA"
     gene            638..2083
                     /locus_tag="VF686_07515"
     CDS             638..2083
                     /locus_tag="VF686_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain-length determining protein"
                     /protein_id="HEX8661700.1"
                     /translation="MRTTAYTTVRPRYGFLDVVGLLFRELLLMIVVFLAVFAIGAAAV
                     LTLKKSYTATASVYAGAGSEYVYQPRVGATERSQPLEADAVARSEAAILNSLEVKRRA
                     VRAVGADTVLGPDASGTAAEKEASALKVLDSSLGVGTTAGSAIISVSYRSDDPVRAAQ
                     VLNAVVDQYLVQRRVVFRDRTTPAIAQQREAFESDLAKADEAYNAFIRSNDIGDFATA
                     KATAAASYTSTFAEVLSLRAQLNQASQRLSTLEAQLAQQPAEVVLQQDLNISAQDQIL
                     QLRTQREALLSRYTPDAQPVRDIEQQIADLQAYVATGATVGPKEVRTGPSDIFTEIET
                     ARITAAADRDGLAARLAVANGQLDQIRSRQAELTRLESTNANLAGNREVLTANIRDFQ
                     QRESAARADSALIAAGADNVTVIERAEAPTRGSSLKAPLLAVIFLFAAFTALCVGLLR
                     IFTRKGYVTPASTGRTLEMPVLAVAPMKAAR"
     gene            2136..>2639
                     /locus_tag="VF686_07520"
     CDS             2136..>2639
                     /locus_tag="VF686_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hfsB"
                     /protein_id="HEX8661701.1"
                     /translation="MVDLTSEMAALWAALGPAPGHRGRVVQIAAANSGEGVSTVAREY
                     ARLAAVRARRPVWLIDADLSQQGQMEAVAGQQDRFGALGKAAIATPDGSIFFAVTPRL
                     MDRAGKPVHPARLLVARPCLGGRLWITKFAAEHLRSGQRAEAVSDARYWDALRKHADT
                     VVVDAPAA"
BASE COUNT          406 a          961 c          870 g          402 t
ORIGIN      
        1 ggcctgataa gcgtccaggt ccccggtcat ttcatagacg ccgggcgtcc gaacctcacc
       61 atccacccag acccggatcg gcccagccgt cttcagcgtc acctcgacca caggacgccg
      121 cagctgggtc gcgtaggcgg cggaggcgtc ggcctcaagt tccgcgatgg tccggtctcc
      181 cgccatgatc tggccgacca gcggcagcga tatccggccg tcgggcccga ccttctgcgt
      241 tcgcgtcaat tcgacggcgg tcggggtggc gatctcgatc tcgtcgccgg gatacaggac
      301 gtattccggt tcggcgtcgc tccagtcggc gaagctgact tccgggaagc cggtccgccc
      361 gacgttccga acgtggttgc cgtcgcgcag gtccgcttgt gaaggcctgc cggcaaagcc
      421 gccgtcgccg agcattccgc cgccgccgcc gcagccgcca accgccgcgc ccaacccggc
      481 aaggcccagc agcatcaggc ggcggtcgat ggacatggag actcattccc gaaaacgtcg
      541 ttgagggcag ggtcgtcgct tatgggtaac catgggttaa cgccggtggc ccaaggcttg
      601 gtctgtcagt cctgttcgat ggaatcagtc cgcccgcatg cgtacgacgg cctacaccac
      661 agtccgcccg cgctacggct tcctcgatgt cgtgggcctg ctgttccgcg aacttcttct
      721 gatgatcgtg gtcttcctgg ccgtgttcgc catcggggcg gcggcggtgc tgacgctcaa
      781 gaaatcctac accgccacgg ccagcgtcta tgcgggggcg ggttcggaat atgtctacca
      841 gccgcgcgtc ggcgctaccg agcgcagcca gccgctggag gccgacgctg tggcccggtc
      901 ggaagccgcc atcctgaaca gcctcgaggt caagcgccgc gcggtccggg ctgtcggcgc
      961 ggatacggtt ctggggcctg acgcttcggg cacggcggcg gagaaggaag cctcggcgct
     1021 caaggttctg gactccagcc tgggcgtcgg caccacggcc ggcagcgcca tcatctcggt
     1081 gtcctacagg tcggacgatc ccgtgcgcgc ggcccaggtg ctgaacgccg tcgtcgacca
     1141 gtatctggtc cagcgccgcg tcgtgttccg cgaccggacc acgcccgcca tcgcccagca
     1201 gcgcgaggcg ttcgagagcg atctggccaa ggccgacgag gcctacaacg ccttcatccg
     1261 gtccaatgac atcggcgact tcgccacggc caaagcgacc gcggccgcca gttatacatc
     1321 gaccttcgcc gaggtgctgt cgctgcgggc ccagctcaat caggccagcc agcggctgtc
     1381 gacgctcgag gcccagctgg cgcagcaacc ggccgaggtc gtgctgcagc aggaccttaa
     1441 catctcggcc caggaccaga tcctgcagct gcgcacccag cgcgaggccc tgctgtcgcg
     1501 ctatacgccc gacgcacagc cggtgcggga catcgaacag cagatcgccg accttcaggc
     1561 ctatgtcgcc accggcgcca ccgtcggacc caaggaagtc cgcacgggcc ccagcgacat
     1621 cttcaccgag atcgaaaccg cccgcatcac cgccgccgcc gaccgcgacg gtctggccgc
     1681 gcgccttgcc gtcgccaacg gccagctcga tcagatccgc tcgcgtcagg ccgagctgac
     1741 ccggctggaa tcgaccaacg ccaacctggc cggcaaccgc gaggtgctga ccgccaacat
     1801 ccgcgacttc cagcagcgcg aaagcgccgc ccgcgccgac agcgccctga tcgccgccgg
     1861 ggccgacaac gtcaccgtca tcgaacgcgc cgaggcgccg acccggggca gcagcctgaa
     1921 ggcgcccttg ctggccgtca tcttcctgtt cgccgccttc accgccctgt gcgtgggcct
     1981 gctgcggata ttcacccgca agggctatgt caccccggcc tcgacgggcc ggacgctgga
     2041 aatgccggtg ctggccgtgg cgccgatgaa ggccgcccga tgattgcgca agccatggta
     2101 gcgtagggac ctgtagccgg gcgcgtagcg attcgatggt cgatctgaca tccgaaatgg
     2161 cggctctctg ggccgcactg ggaccggcgc ccggccatcg cggccgcgtg gtgcagatcg
     2221 ccgccgccaa ctcgggcgag ggcgtctcga ccgtggcccg cgaatacgcc cgtctggccg
     2281 ctgtccgcgc gcgccgtccc gtctggctga ttgacgccga cctgtcgcag caggggcaga
     2341 tggaggccgt ggcgggccag caggaccggt tcggcgcctt gggcaaggcc gcgatcgcca
     2401 cgccggacgg ttcgatcttc ttcgccgtca cgccgcgcct gatggaccgc gccggcaagc
     2461 ccgtgcatcc cgcgcgcctg ctggtggcgc ggccctgcct cggcgggcgg ctgtggatca
     2521 cgaaattcgc cgccgagcat ctgcgctcgg gccagcgcgc cgaggccgtc agcgacgccc
     2581 gctactggga cgccctgcgc aaacatgctg acacggtggt ggtcgacgcc ccggcggct
//
LOCUS       DASVVN010000468         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_491370,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000468 DASVVN010000000
VERSION     DASVVN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491370"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1167
                     /locus_tag="VF686_07525"
     CDS             40..1167
                     /locus_tag="VF686_07525"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8661702.1"
                     /translation="MSEQTTFTEADRTDGIEPDMADQNHAPKSQAPEAAAVMPMNMIQ
                     ALNSALHVKMAEDPNVLSFGEDAGYFGGVFRVTDQLQQKHGLTRSFDAPISECGIVAA
                     AIGMGAYGLRPVVEIQFADYIYPAYDQIVSEAAKLRYRSGGQFTSPITVRSPYGGGIF
                     GGQTHSQSPESLFTHIAGLKVVIPSNPYDAKGLLTAAIEDDDPVIFFEPKRLYNGPFD
                     GWHQKPVSPWKAQELAQVPTGKYVEPIGKARIMREGADVTILCYGTMVWVSLAGAEHA
                     GVDAEVIDLRTLVPLDIETIEASVKKTGRCVIVHEAPKTSGFGGELSALVQERCFYHL
                     EAPIARVTGWDTPYPHAFEWEYFPGPERVATALKAVMTGGR"
     gene            1169..2884
                     /locus_tag="VF686_07530"
     CDS             1169..2884
                     /locus_tag="VF686_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8661703.1"
                     /translation="MGRYVFKLPDVGEGTAEAELVGWHVKVGDMVAEDQIIADIMTDK
                     ATVELTSPVAGKVLAAHGEPGQQLAVGSPLVEFEVEGAGNASEAPPPSNPAPQASVPL
                     TGGQTAAAAAPTVSSALRGEGDPEGVEGRQAAQTSGNYVFKLPDVGEGTAEAELVGWH
                     VKVGDTVAEDQLLAEVMTDKATVELTTPVAGTVAALHGEAGQQIAVGGPLVSFNVEGK
                     GNVAAASPSTASRSPSPQGGEETVRPAPPPPSVPLSGGPNPAEQGWRGAAPQRQLSAR
                     NERPLASPAVRNRARDLGIELQFVPGSGPAGRIEHSDLDAYVASGGRGSSVSGASAGT
                     TYARAEGTTETRIIGLRRKIAEKMAESVRRIPHITYVEEIDVTALEELRAHLNATKKK
                     DQPKLNVLPFIARAIVVALRDQPTINSHYDDEAGVLTTHNAVHLGIAAQTPNGLMVPV
                     VRNAEARDPYDTANEIARVSGAAKDGSAKRDELSGSTITITSLGTLGGITHTPIINHP
                     EVAIVGPNKIAERVVVKDGQMVVRKMMNLSSSFDHRIVDGHDAAVFVQRIKGLLEHPA
                     TLWMN"
     gene            2890..4287
                     /gene="lpdA"
                     /locus_tag="VF686_07535"
     CDS             2890..4287
                     /gene="lpdA"
                     /locus_tag="VF686_07535"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8661704.1"
                     /translation="MADAIKPKVLIIGAGTGGYVAGIRCGQLGLDTVLVDGGDGLGGT
                     CLNVGCIPSKAIIHAASKFETVSRAADGGTLGITAAKPAIDLSQTVAWKDGIVKKLNN
                     GVAALLKKSKVKVIKGWAEFSDAKTCAVKTADGDIVITAEHVILATGSEPVELPFLKF
                     GGDVISSTEALSLSAVPKKLVVVGGGYIGLELGIAFRKLGAEVAIVEMADRLLPLYDK
                     ALTDPVAKWLEKHGVELHLGAKAGSFENGQLNVTTKDGAAISLQADKVLVTVGRRPRT
                     RGWGLENMGVAMAGPFVKIDDRCATSMKNVWAVGDLTGEPMLAHKGSAQGEVVAEIIA
                     GHDRRFDPVTIAAVCFTEPEIVSAGLGPLDVEGRDDVITAVFPLAAIGRALAIEAGED
                     GGFVRVLASKGDHRLLGVQAVGQHVAEMSNSFAQMLEMGAVLEDVAGVIHVHPTLGEA
                     FHEASLRALGHAIHI"
     gene            4485..>4749
                     /locus_tag="VF686_07540"
     CDS             4485..>4749
                     /locus_tag="VF686_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8661705.1"
                     /translation="MTRTLLAAASAIALLAVAGCGNDQPAGSQTARAGIWAAGSSTVF
                     PFATRVAENFARTSGGAAPRVESLGTGGGIQAFCQGVGPNTPDI"
BASE COUNT          810 a         1741 c         1512 g          686 t
ORIGIN      
        1 ctggctcccg gccttcgccg ggatgagcgg tgaattctga tgagcgagca aaccaccttc
       61 accgaagccg accgcaccga cggcatcgag ccggacatgg ccgaccagaa ccacgcgccg
      121 aagtcgcagg cgcccgaggc cgccgcggtc atgccgatga acatgatcca ggcgctgaac
      181 tcggccctgc acgtcaagat ggccgaggat cccaacgtcc tcagcttcgg cgaggacgcc
      241 ggctatttcg gcggcgtctt ccgcgtcacc gaccagctgc agcagaagca cggcctgacc
      301 cgaagcttcg acgccccgat cagcgaatgc ggcatcgtcg ccgccgccat cggcatgggc
      361 gcctacggcc tgcgccccgt cgtcgagatc cagttcgccg actacatcta tccggcctac
      421 gaccagatcg tctccgaggc ggccaagctg cgctaccgct cgggcggcca gttcacctcg
      481 ccgattacgg tgcgttcgcc ctacggcggc ggcatcttcg gcggccagac ccacagccag
      541 tcgccggaaa gcctgttcac ccacatcgcc gggctgaagg tcgtcatccc gtccaacccc
      601 tatgacgcca agggcctgct gacggcggcg atcgaggacg acgatccggt catcttcttt
      661 gagcccaaac gcctctacaa cggccccttc gatggctggc accagaagcc ggtctcgccg
      721 tggaaggcgc aggaactggc ccaggtcccg accggcaaat acgtcgagcc gatcggcaag
      781 gcgcgcatca tgcgcgaggg cgctgacgtc accatcctct gctacggcac catggtctgg
      841 gtctcgctgg cgggcgccga gcacgccggc gtcgacgccg aggtcatcga cctgcgcacg
      901 ctcgtgccgc tggacatcga gaccatcgag gccagcgtca agaagaccgg ccgctgcgtc
      961 atcgtccacg aggccccgaa aacctctggc tttggcggtg aactgtcggc gttggtgcag
     1021 gagcgctgct tctatcatct ggaggcgccc atcgcgcggg tgacgggctg ggacaccccc
     1081 tatccgcacg ccttcgagtg ggaatatttc ccgggacccg agcgggtcgc cacggccctg
     1141 aaggccgtca tgaccggagg gcgctaggat gggccgctac gtcttcaaac tgcccgacgt
     1201 gggcgaaggc accgccgagg ccgagctggt cggctggcac gtcaaggtcg gcgacatggt
     1261 cgccgaggac cagatcatcg ccgatatcat gaccgacaag gccacggtcg agctgacctc
     1321 gccggtcgcc ggcaaggtgc tggccgccca cggcgaaccg ggccagcagc tggcggtggg
     1381 ttcgccgctg gtcgagttcg aggtcgaggg cgcggggaat gcgagtgagg cgccgccccc
     1441 ctcgaaccct gctccgcagg cttcggtccc cctgacaggg ggacagacgg cggctgcggc
     1501 tgcgcccacc gtctcctccg cgctccgcgg ggagggggac cccgaagggg tggaggggcg
     1561 ccaagccgca caaacctccg gcaactacgt cttcaaactc cccgacgttg gcgaaggcac
     1621 ggcagaggcc gaactggtcg gttggcacgt caaggtcggc gacaccgtcg ccgaggacca
     1681 gctgctggcc gaggtcatga ccgacaaggc gacggtcgag ctgacgacgc cagtcgccgg
     1741 aaccgtcgcc gccctgcacg gcgaggccgg ccagcagatc gccgtcggcg ggccgctggt
     1801 cagcttcaac gttgagggca agggcaatgt cgctgcggcg agcccctcca ccgcttcgcg
     1861 gtccccctcc ccgcaaggcg gggaggagac ggttcggccg gcgccgccgc cgccgtctgt
     1921 ccccctgtca gggggaccga atcctgcgga gcagggttgg aggggggcgg cgcctcaacg
     1981 ccagctctcc gcccgcaacg agcgtccgct cgcctcgccc gccgtccgca accgcgcccg
     2041 cgacctcggc atcgagctcc agttcgtccc cggctccggc cccgccggcc gcatcgaaca
     2101 ttccgacctg gacgcctacg tcgcctcggg cggccgcgga tcgtccgtgt ccggagcatc
     2161 ggccggcaca acctacgcca gggccgaggg aaccaccgaa acccgcatca tcggtctgcg
     2221 ccgcaagatc gcggagaaga tggccgagag cgtccgccgc attccccaca tcacctatgt
     2281 ggaggagatc gacgtcaccg ccctcgagga actccgcgcc cacctcaatg cgacgaagaa
     2341 gaaggaccag cccaagctca acgtcctgcc cttcatcgcc cgcgccatcg tcgtggccct
     2401 gcgcgaccag ccgacgatca actctcacta cgacgacgag gccggcgtcc tgacgaccca
     2461 caacgccgtc cacctcggca tcgccgccca gacgccgaac ggtctgatgg tgccggtggt
     2521 ccgcaacgcc gaggcgcggg acccctacga caccgccaat gagatcgccc gcgtctcggg
     2581 cgcggccaag gacggctcgg ccaagcgcga cgaactgtcg ggctcgacca tcaccatcac
     2641 ctcgctgggg accttgggcg gcatcaccca tacccccatc atcaaccacc ccgaggtcgc
     2701 catcgtcggc cccaacaaga tcgccgaacg cgtggtggtc aaggacggcc agatggtcgt
     2761 gcgcaagatg atgaacctgt cctccagctt cgatcaccgc atcgtcgacg gccacgacgc
     2821 ggccgtcttc gtccagcgta tcaagggcct gctggagcat cccgcgaccc tctggatgaa
     2881 ctgagaacca tggctgacgc gatcaaaccc aaagtcctga tcatcggcgc cggcaccggc
     2941 ggctatgtcg cgggcatccg ctgcggccag ctgggcctcg acaccgtgct ggtcgacggc
     3001 ggcgacggcc tcggcggcac ctgcctgaac gtcggctgca tcccctccaa ggcgatcatc
     3061 cacgccgcct cgaaattcga gaccgtgtcg agggccgccg atggcggcac cctgggcatc
     3121 accgccgcca agccggccat cgacctgtcg cagacggtgg cgtggaagga cggcatcgtc
     3181 aaaaagctga acaacggcgt cgccgccctg ctgaagaagt cgaaggtcaa ggtcatcaag
     3241 ggctgggccg agttctcgga cgcgaagacc tgcgcggtga agaccgccga cggcgacatc
     3301 gtcatcaccg ccgagcacgt tatcctcgcc accggctccg aaccggtcga gctgcccttc
     3361 ctgaagtttg gcggcgacgt catctcctcg accgaggccc tgtcgctgtc tgcggtgccg
     3421 aagaagctgg tcgtcgtcgg cggcggctat atcggcctcg aactgggcat cgccttccgc
     3481 aaactcggcg cggaagtcgc cattgtcgaa atggccgacc gcctgctgcc cctttacgac
     3541 aaggccctga ccgatccggt cgcgaaatgg ctggagaagc acggtgttga actgcacctc
     3601 ggcgccaagg ccggctcgtt cgaaaacggc cagctcaacg tcaccacgaa ggacggcgcg
     3661 gccatttcgc tccaggccga caaggtcctc gtcaccgtcg gccgtcgtcc ccgcaccagg
     3721 ggctggggtc tggagaacat gggcgtcgcc atggccggtc cgttcgtgaa gatcgacgac
     3781 cgctgcgcca ccagtatgaa gaacgtctgg gccgtcggcg acctgaccgg cgaacccatg
     3841 ctggcccaca agggcagtgc gcagggcgag gtcgtcgccg agatcatcgc gggccacgat
     3901 cgccgcttcg accccgtcac catcgccgcc gtctgcttca ccgagcccga gatcgtctcc
     3961 gccggcctcg gcccgctgga cgtggagggc cgcgacgacg tcatcacggc cgtcttcccg
     4021 ctggccgcca tcggccgcgc cctcgccatc gaggccggcg aggacggcgg cttcgtccgc
     4081 gttctggcgt ccaaaggcga ccaccgcctg ctcggcgtcc aggccgtcgg ccagcacgtc
     4141 gccgaaatgt ccaacagctt cgcccagatg ctggaaatgg gcgcggtgct ggaagacgtc
     4201 gccggcgtca tccacgtcca tccaaccctc ggcgaagcct tccatgaggc gtccctgcgc
     4261 gccttgggac acgcgattca catctaggct gcttcagatt gtcgtcatcc cggggcgcga
     4321 agcgaccccg ggacccagcg gcgccggagc gaagcggagg cgacgggaga aacggctgcg
     4381 ctggcgggag ccaagccgtc gccgaaccgt cacatcacca tcggcctccc cgtcacaaac
     4441 ctctggtcta agcgcctcac cttggctttg tccggagact gacgatgacc cgcactcttc
     4501 tcgccgccgc ctcggcgatc gccctgctcg ccgtcgccgg ctgcggcaac gatcagcccg
     4561 ccggctccca gaccgcccgc gccggcatct gggcggcggg ctcctcgacc gtcttcccct
     4621 tcgccacccg cgtggccgag aatttcgccc gcacctcggg cggcgccgcg ccccgcgtcg
     4681 aatcgctggg caccggcggc ggcattcagg ctttctgcca gggcgtcggt cccaacaccc
     4741 ctgacatcg
//
LOCUS       DASVVN010000469         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_17645, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000469 DASVVN010000000
VERSION     DASVVN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17645"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF686_07545"
     CDS             <1..230
                     /locus_tag="VF686_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX8661706.1"
                     /translation="GAITFAEFQGHHDAMMRASFRTLDRSGDGRVDRSEFRATFSAGE
                     AEPSAEEPAPTFDHLDTNKDGFLSLQELHGS"
     gene            complement(441..923)
                     /locus_tag="VF686_07550"
     CDS             complement(441..923)
                     /locus_tag="VF686_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX8661707.1"
                     /translation="MSWTDDRVTVLKKLWLEGLSASQIAKQLGGVTRNAVIGKVHRLG
                     LSGRAAPSQPQRAVFKAPRPPRPVSSSPVIRREPIIRPEPVRLREPEVPGSATVLTLG
                     AHMCKWPIGDPSSDEFTFCGQRTEGTYCSQHSRVAYQPQQNRKRVGASDLVRSLRRYM
                     "
     gene            1157..>1783
                     /locus_tag="VF686_07555"
     CDS             1157..>1783
                     /locus_tag="VF686_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8661708.1"
                     /translation="MSVSDAPVRPPEPRRYHGVNWVGLRTLYLREVRRFWKVGTQTVA
                     APVVTTLLYMLIFVVAMQGATPPVHGVPFSQFVAPGLVMMAILNNAFANASSSLIQAK
                     LMGTAPDFLTPPLSPFEQAAGFALGAATRGVLVGVVTAIAVFFFAEARISHLWAVVYF
                     GLAASFMMGLVGVVAGLWSEKFDHLAAVTNFIIVPMTFLSGTFYLIERL"
BASE COUNT          251 a          612 c          622 g          298 t
ORIGIN      
        1 acggcgccat cacgttcgcc gagttccagg gccaccacga tgcgatgatg cgcgccagct
       61 tccgcacgct cgaccggagc ggcgacggac gggtcgaccg ctccgagttc cgggcgacgt
      121 tctcggcggg cgaagccgag ccgtccgccg aggaaccggc gccgacgttc gaccatctgg
      181 acacgaacaa ggacggcttc ctgagtttgc aggagctgca cggctcttga ggcgcctcct
      241 ccctcgtacc gtgcgcgacg ggggaggact cgtcgtgcat gcgacgagcc gccgcggacg
      301 gtagcgctga agtacggccc gcggtcgggc cggcaatcct acctcgtcag cccacaaaaa
      361 gaggggcccg aaggcccctc agatgcgtcg gatgcggtcc cgcgaagggg ggaacgcggc
      421 aagccgcacc cttccgcgga ctacatgtag cggcgcaggc tccgcaccag gtcgctggcg
      481 ccgacgcgct ttctgttctg ctgcggctgg taggccacgc gcgaatgctg cgagcagtag
      541 gtgccttcgg tgcgctggcc gcagaaggtg aactcgtcgc tggacgggtc gccgatcggc
      601 cacttgcaca tgtgggcgcc gagggtcagc acggtggcgc tgcccggaac ctccggctcc
      661 cgcaggcgca ccggctccgg cctgatgatc ggctcgcggc ggatcaccgg cgaggaggac
      721 accgggcgcg gcggccgcgg cgccttgaac acggcgcgct gcggctgcga gggcgcggcg
      781 cggcccgaca gcccgagccg gtgcaccttg ccgatgaccg cgttgcgggt gaccccgccc
      841 agttgcttgg cgatctggct ggccgacagg ccttcgagcc agagcttctt caggaccgtc
      901 acgcggtcgt ccgtccaact cattgaagcc tcccgccggc ccctccggca ttggactaga
      961 tcttgtgtgg tgagcgccgt ctcgatcggc ctcacgcaag atagagttac ccagaaggtt
     1021 aacggccaca ataggtggcg cccggtctgt ccacaggaaa ctagatgtag tgcccaaggt
     1081 aggcccgtgg gtggggccgg gtagcgccgc ggtcggaccg gttgcgcgcg cggtcgcacc
     1141 ggcgcacatc gggcgcatgt cagtctcaga cgcccccgtc cgcccgcccg agccgcgccg
     1201 ctatcacggc gtcaattggg tgggcctgcg cacgctgtac ctgcgcgagg tgcgccgctt
     1261 ctggaaggtc ggcacccaga cggtggcggc gccggtggtg accacgctgc tctacatgct
     1321 gatcttcgtg gtcgcgatgc agggcgcgac gccgccggtg cacggcgtgc cgttctcgca
     1381 gttcgtcgcg cccggcctgg tcatgatggc catcctcaac aacgccttcg ccaacgcctc
     1441 ctcctcgctg atccaggcca agctgatggg cacggcgccg gactttctga ctccgccgct
     1501 ttcgccgttc gagcaggcgg ccggctttgc gctcggcgcg gcgacgcgcg gcgtgctggt
     1561 gggcgtggtg acggcgatcg ccgtcttctt tttcgccgag gcgcggatca gccacctctg
     1621 ggcggtggtc tacttcggcc tcgccgcctc gttcatgatg gggctggtgg gcgtcgtcgc
     1681 cggcctgtgg tcggagaagt tcgaccacct cgccgcggtg accaacttca tcatcgtgcc
     1741 gatgaccttc ctgtcgggca ctttctacct gatcgagcgg ctg
//
LOCUS       DASVVN010000470         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_517697,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000470 DASVVN010000000
VERSION     DASVVN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517697"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VF686_07560"
     CDS             <1..1232
                     /locus_tag="VF686_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX8661709.1"
                     /translation="FSSVAAVGFALTCWPIGVERGWMSREEARERTVTTIRFFHDAPQ
                     GPQATGVTGYKGFFYHFLDMETGHRFGRTELSTVDTTLLVAGMLFAARWFDGDHADEA
                     EIRRLAQAVYDRIEWDWAVVRPNRISMGWHPETGFIPADWHIYNEGMLVLLLAIGSRT
                     HPVPAAVWGEWCSVYDQSWTDRWGPAHLAFAPLFGHQYSHMFVDFRGIHDPWIRAKVR
                     ETGEAFDYRINSERAVQAQRKYAIDNPMGWAGYSADVWGLTACDGPGDFKQVIEGRER
                     EFFSYSARGPGDRDDGTIAPTAAMASYPFAPAEVTACLKAMKARYGAGIYTEFGFMDS
                     FNPTLTRRDGRLQHGRIVPNVGWVDGDYLGIDQGPIVIQIENQRSDAVWRRMRAEPNI
                     VRGLKRAGFSGGWLDRI"
     gene            1229..2503
                     /locus_tag="VF686_07565"
     CDS             1229..2503
                     /locus_tag="VF686_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8661710.1"
                     /translation="MTAVRPDRRTALGLLAGAATGLAGCGGRNDGVTRLRFWAMGNEA
                     TNVPQILPGFERRNPGIKVEVQALPWTAAHSKLLTSYAGNSLPDLSQVGNTWVSEMAA
                     IGAISALPAFASDLLTDQFPAVLETNRIGGQVVGVPWYVDTRLIYYRADLLERAGFGD
                     GVPQTWETWKAAMHAVKRVAGPGNYAVLLPVNEFEQLLTFGLQGAEPLLRDRDTRGNF
                     SSASFIAALEFYKSLFDEGLAPVASSTQISNVWTEFERGYFSFYFSGPWTIGDFKNRL
                     KPTTRWMTSGVPGPTGPGASAPGGSSLVVYRHSPHQEAAWKLVRYLADPAVQARFNTI
                     TGDLPARESAWATPAVADDPYIAAFKGQLSRAKAVPKVPEWERIVSEMQIVAERMVRG
                     EYTPAAAAAEIDRRADRLLEKRRWMIGQGRAI"
     gene            2500..>2685
                     /locus_tag="VF686_07570"
     CDS             2500..>2685
                     /locus_tag="VF686_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661711.1"
                     /translation="MTDVPASADTIIARPAPRPARNARNRERAAWGFVAPALIAIGVF
                     FAIPVFSALLLSLTDFDI"
BASE COUNT          405 a          909 c          931 g          440 t
ORIGIN      
        1 cattctcgtc ggtggccgcg gtcggcttcg ccctgacctg ctggcccatc ggggtcgagc
       61 gcggctggat gagccgggag gaggcgcgcg agcgcaccgt gaccacgatc cgcttcttcc
      121 acgacgcgcc gcaggggccg caggccacgg gcgtgaccgg gtacaagggc ttcttctacc
      181 acttcctcga catggagacc ggacaccgct tcggccgcac cgaactgtcc acggtcgaca
      241 ccacgcttct ggtcgccggc atgctgttcg cggcccgctg gttcgacggc gaccatgccg
      301 atgaggccga gatccggcgt ctggcgcagg ccgtctatga ccggatcgag tgggactggg
      361 ccgtcgtgcg tccgaaccgc atctcgatgg gctggcatcc ggagaccggg ttcatccccg
      421 cggactggca catctacaac gagggcatgc tggtcctgct gctggccatc ggcagccgca
      481 cccatccggt tcccgcggcc gtctggggcg aatggtgcag cgtctacgac cagagctgga
      541 ccgaccgttg ggggccggcg cacctggcct tcgccccgct gttcggccac cagtacagcc
      601 atatgttcgt cgacttccgc ggcatccatg acccgtggat ccgggcgaag gtccgcgaga
      661 cgggcgaggc gttcgactac cgcatcaaca gcgagcgcgc ggtgcaggcc cagcgcaaat
      721 acgccatcga caatccgatg ggctgggccg ggtattcggc ggatgtctgg ggcctgacag
      781 cctgcgacgg gccgggcgat ttcaaacagg tcatcgaggg gcgcgagcgc gagttcttca
      841 gctattcggc gcgcggcccc ggcgaccgcg acgacggcac catcgccccg acggcggcca
      901 tggcctccta tcccttcgcc ccggccgagg tgaccgcctg tctgaaggcg atgaaggcgc
      961 gctacggggc cggcatctac accgagttcg gcttcatgga ctcgttcaac ccgacgctga
     1021 cccggcgcga tggccgtctg cagcacggac gcatcgtgcc gaacgtgggc tgggtcgacg
     1081 gcgactatct gggcatcgac caagggccga tcgtcatcca gatcgagaat cagcggtccg
     1141 atgcagtgtg gcgccggatg cgggctgagc ccaacatcgt tcgtggcctg aagcgtgccg
     1201 gattctcggg cggctggctg gatcgcatat gacggctgtg cggccggacc gtcggacggc
     1261 gctgggcctt ctggccgggg cggcgacggg gctggccggt tgtggcggcc gcaacgacgg
     1321 cgtcacccgc ctgcgcttct gggccatggg caatgaggcg accaacgtcc cgcagatcct
     1381 gcccgggttc gagcggcgca atcccgggat caaggtcgag gttcaggccc ttccatggac
     1441 ggcggcccac tccaaacttc tgacttcata cgccggtaat tcgctgcccg atctcagcca
     1501 ggtgggcaac acctgggtgt ccgagatggc cgccatcggg gcgatcagcg ccctgccggc
     1561 cttcgcttcg gacctgctga ccgaccagtt ccccgccgtg ctggagacca accgcatcgg
     1621 cggccaggtc gtgggcgtgc cctggtacgt cgacacccgg ctgatctact accgcgccga
     1681 tctgctggaa cgggccggct ttggcgacgg cgtgccccag acctgggaga cgtggaaggc
     1741 ggccatgcac gcggtcaagc gcgtggcggg gccggggaac tatgctgtcc tgctgccggt
     1801 caacgagttc gaacagttgc tgaccttcgg cctgcagggc gccgagccgc tgctgcgcga
     1861 tcgcgacacg cggggcaatt tctccagcgc ctccttcatc gccgccctgg aattctacaa
     1921 gagcctgttc gacgaggggc tggctccggt ggcgtcctcg acgcagatct ccaacgtctg
     1981 gaccgagttc gagcgggggt atttcagctt ctacttctcg gggccgtgga ccatcggcga
     2041 cttcaaaaac cggctgaagc cgacgacgcg ctggatgacc tcgggcgtgc cggggccgac
     2101 ggggccgggg gcgtcggcgc cgggcggatc ctcactggtc gtctaccgcc attcgccgca
     2161 tcaggaggcg gcgtggaagc tggtgcgcta tctggccgat ccggcggtgc aggcgcggtt
     2221 caacaccatc accggcgacc tgccggcgcg cgagagcgca tgggccacgc cggcggtggc
     2281 cgacgacccc tatatcgccg ccttcaaggg ccagctgtca cgggccaagg ccgtgcccaa
     2341 ggtgccggaa tgggagcgga tcgtctcgga gatgcagatc gtcgccgagc ggatggtccg
     2401 gggtgaatac acgcccgccg ccgccgccgc cgagatcgac cgccgggccg accggctgct
     2461 ggaaaagcgc cgctggatga tcgggcaggg gagggcgata tgaccgatgt tccggcctcg
     2521 gccgacacta tcatcgccag acccgcgcct cgtccggcgc gtaacgcccg taaccgcgag
     2581 cgcgcggcct gggggttcgt ggcgccggct ctgatcgcca tcggcgtctt cttcgccatt
     2641 ccggttttct cggccctgct gctgagcctg accgacttcg acatc
//
LOCUS       DASVVN010000471         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_70658, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000471 DASVVN010000000
VERSION     DASVVN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70658"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VF686_07575"
     CDS             <1..727
                     /locus_tag="VF686_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661712.1"
                     /translation="PAQRQQMIARILADPATAATFPGADAAAGLIVDTIGKDSAAMLL
                     IANAVEEDAYTIQGRSDPRRRWATIHITDRVTRLEGAKSLSAVTMLPSAEESARLFAA
                     ANSGTGLGTGSARSGPPYTAAVVRSLAVAALAALGAGGEDGRANTEALTVEQNSEFCL
                     NMSKLMLFQCLAASRPSYEDMFCVGRHVARDLATCTAQNITPIPVEIPQTAVAGGAPE
                     QTPVAAEAAAASTAATASLNNGG"
     gene            866..>1510
                     /locus_tag="VF686_07580"
     CDS             866..>1510
                     /locus_tag="VF686_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX8661713.1"
                     /translation="MAEIILNVDVRDGVGTGGARAARRAGMVPGVLYGGDKAPVAISV
                     NERDFRKSLYTGKLLGHLVTLKYGDESQPVIARDVQFDPVSDRPLHFDLMRVDEKQTI
                     KIEIPVHFINQDQCEAFRQGGSLEIVRHSVEVKVRADHIPEDLIVDLAGHKMGDTIRW
                     TDLKGTEDVEPTITDRDFVIASIKTSSAAISAAADEAHDAEVAEAAAEVEATEQG"
BASE COUNT          247 a          545 c          494 g          224 t
ORIGIN      
        1 tcccgcccag cgtcagcaga tgatcgcccg cattctcgcc gacccggcga cggctgcgac
       61 cttccccggc gcggacgccg ccgccggcct gatcgtcgac accatcggca aggattcggc
      121 cgccatgctg ctgatcgcca acgcggtcga ggaggacgcc tatacgatcc agggccgcag
      181 cgatccgcgc cgccgctggg ccaccatcca catcaccgac cgcgtcaccc ggctggaggg
      241 cgccaagtcg ctgtccgccg tgaccatgct gccttcggcc gaggaatcgg cccgcctgtt
      301 cgccgccgcc aacagcggta cgggtctggg cacgggttcg gcgcgctcgg gtccgcccta
      361 taccgccgcc gtggtccggt cgctggccgt cgccgccctg gccgctctcg gcgccggggg
      421 cgaggacggc cgcgccaaca ccgaggccct gacggtcgag cagaacagcg aattctgcct
      481 caacatgtcc aagctgatgc tgttccagtg tcttgccgcc tcgcgcccca gctatgagga
      541 catgttctgc gtcggccggc acgtggcgcg cgatctggcc acctgcacgg cccagaacat
      601 cacgccgatc ccggtcgaaa tcccccagac cgccgtggcg ggcggggctc cggagcagac
      661 gccggtcgcc gccgaggcgg ccgccgcttc gactgcggcg acggcgtcgc tgaacaacgg
      721 cggctaaacc gcgcaagcga cgccagcggc ctgttgcgtc gcggggttgt tgcgtgtaat
      781 gacccgcgct cttgaattca gggtccctga ccccgccgcg agggctgact ggtcagcgcg
      841 gcgtttgtgt tgttgaggcg aggcgatggc cgagatcatt ctgaacgtgg acgtccgtga
      901 tggcgttggc accggtggcg cacgcgccgc gcgtcgcgcg ggcatggtcc cgggcgtgct
      961 gtacggcggc gacaaggccc ccgtcgccat ctcggtgaac gagcgcgact tccgcaagtc
     1021 gctctacacc ggcaagctgc tgggccacct ggtgaccctg aagtacggtg acgagagcca
     1081 gccggtcatc gctcgcgatg tccagttcga cccggtcagc gaccgtccgc tgcacttcga
     1141 cctgatgcgg gtcgacgaaa agcagaccat caagatcgaa atcccggtcc acttcatcaa
     1201 ccaggaccag tgcgaagcct tccgtcaggg cggctcgctg gaaatcgtgc gccactcggt
     1261 cgaggtgaag gtccgcgccg accacatccc ggaagacctg atcgtcgacc tcgccggcca
     1321 caagatgggc gacaccatcc gctggaccga cctgaagggc acggaagacg tcgagccgac
     1381 catcaccgac cgcgacttcg tgatcgcctc catcaagact tcttcggccg ccatctcggc
     1441 cgccgccgac gaagcgcacg acgctgaagt ggccgaggcc gccgccgagg tcgaagcgac
     1501 cgaacagggc
//
LOCUS       DASVVN010000472         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_149464,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000472 DASVVN010000000
VERSION     DASVVN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149464"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VF686_07585"
     CDS             <1..895
                     /locus_tag="VF686_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX8661714.1"
                     /translation="TAESEAGEADRAARAAESAASTAREARAGLAARAEAAAERLAEA
                     TANVRETAQMSPEELGQKLTDDAIARPPDAAGAESLLFGLEREREALGAVNLRAEDEA
                     VEYGDRLNTMKVERSDLTSAIAKLRDGIDELNAEGRERLVAAFDVINDNFKSLFEALF
                     GGGQAELKLVESDDPLEAGLEIYACPPGKRLAVMSLMSGGEQALTAAALIFAVFLANP
                     APVCVLDEVDAPLDDANVDRFCRMLDEMRKRTDTRFIVITHNPVTMSRMDRLYGVTMP
                     ERGVSQLVSVDLNQAEEIVAA"
     gene            complement(892..1617)
                     /locus_tag="VF686_07590"
     CDS             complement(892..1617)
                     /locus_tag="VF686_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX8661715.1"
                     /translation="MTRFMTLDFAGGDRSAMLDRVIGMARAPYGYVVTPNVDHVIKLM
                     DGRISRDIYDGADLKICDSRILAHLARLRGLTLPVYPGSDMTADLLAASAAEDLVIAV
                     FGPDRAAFDDLAARYPRQTLRWIEAPMLTPGTAGWTAAVRAAAKAEWDVLLACVSFPK
                     QELFAHALRAAGRKSGVTLCVGASVDFLTGRQQRAPRIFQQLSLEWLHRLLSQPRRMF
                     RRYVIEGPAIFGWFVKTELMGSR"
     gene            1665..2129
                     /locus_tag="VF686_07595"
     CDS             1665..2129
                     /locus_tag="VF686_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEX8661716.1"
                     /translation="MKTALPAILTAILLAAPACAQPQPGPRLVLTGVGRFAMLADLST
                     ISRDGDRVRMRSLQVTEEDFQAGGQTYWGGWSWWSFDCRARTADRLDFASVREGGGEG
                     PATPDTAPAYEAAAGGDAAELLAVACDPASAGEADADNLADAVRIGRAALAG"
     gene            complement(2098..>2529)
                     /locus_tag="VF686_07600"
     CDS             complement(2098..>2529)
                     /locus_tag="VF686_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8661717.1"
                     /translation="EITSTYVVVKLWDWRRMILPLTYFITKPFQNWTRETASLIGSAF
                     LYVDYEAPMDRLRAELERICRDSPLWDGDVVNLQVTDITDRVAQVRCLASARNAPTAF
                     DLRCEIREKMLAFMRDQCPEALPKDRLALEQAIRPEPLARS"
BASE COUNT          425 a          828 c          927 g          349 t
ORIGIN      
        1 tacggccgag agcgaggccg gcgaggccga ccgggcggcg cgggcggccg agagcgcggc
       61 ctccacggcg cgcgaggccc gggccggtct ggcggcgcgg gcggaagcgg cggcggaacg
      121 gctggccgag gctaccgcca atgtccgcga gaccgcccag atgtcgcccg aggagctggg
      181 ccagaagctg accgacgacg ccatcgcccg gccgcccgac gccgccgggg ccgaaagcct
      241 gctcttcggt ctggagcggg aacgcgaggc gctgggcgcc gtcaacctgc gcgccgagga
      301 cgaggcggtc gaatacggcg accggctgaa caccatgaag gtcgagcgct cggacctgac
      361 cagcgccatc gccaaactgc gcgacggcat cgacgaactg aacgccgagg gccgcgaacg
      421 gctggtggcg gcgttcgacg tcatcaacga caacttcaag agcctgttcg aggccttgtt
      481 cggcggcggc caggccgagc tgaaactggt cgagagcgac gatccgctgg aggccgggct
      541 ggagatctac gcctgcccgc cgggcaagcg gctggcggtg atgagcctga tgtcgggcgg
      601 cgaacaggcc ctgacggcgg cggccctgat cttcgccgtc ttcctggcca atccggcccc
      661 ggtgtgcgtg ctggacgaag tcgacgcccc gctggacgac gccaacgtcg accgcttctg
      721 ccgcatgctg gacgagatga ggaagcggac ggacacgcgc ttcatcgtca tcacccacaa
      781 tccggtgacc atgagccgga tggaccgcct ctacggcgtc accatgcccg agcgcggcgt
      841 gagccagctg gtcagcgtcg acctgaacca ggcggaagag atcgtagcgg cctagcgcga
      901 ccccatcagt tcggttttca cgaaccaccc gaagattgcc gggccctcga tgacatagcg
      961 gcggaacatg cggcggggct ggctgagcag acggtgcagc cattccagcg acagctgctg
     1021 gaagatgcgc ggggcccgct gctggcgacc ggtcaggaag tccaccgagg cgccgacaca
     1081 cagggtcacg ccagacttgc ggccggcggc gcgcagggcg tgggcgaaca gctcctgttt
     1141 ggggaaggag acgcaggcca gcagcacatc ccactcggcc ttggcggcgg cgcggacggc
     1201 ggctgtccag cccgccgtcc cgggcgtcag catcggggcc tcgatccagc gcagggtctg
     1261 gcgcggatag cgggccgcca gatcgtcgaa ggctgcgcgg tcggggccga aaacggcgat
     1321 gaccagatcc tcggcggcgg aggcggccag caggtcggcg gtcatgtcgg accccggata
     1381 gacgggcagg gtcaggccac gcaggcgggc cagatgagcg aggatgcggc tgtcgcagat
     1441 cttcagatcg gcgccgtcgt agatgtcgcg cgaaatccgt ccgtccatca gcttgatgac
     1501 gtgatcgaca ttgggcgtga cgacatagcc gtagggcgcg cgcgccatgc cgatgacgcg
     1561 gtccagcatc gcggaccggt ccccgccggc gaagtcgagc gtcatgaagc gcgtcatggc
     1621 gaacagcgta gtgcgccgcc gtcaacgcgc ggttacgatg gcgcatgaaa accgccctgc
     1681 ccgccatcct gaccgcaatc ctgctggccg cgcctgcctg cgcccagccg cagccgggtc
     1741 cgcgactggt cctgaccggc gtgggccggt tcgcgatgct ggctgacctg tccacgatct
     1801 ctcgcgacgg cgaccgggtg cggatgcgca gcctgcaggt gacggaagaa gactttcagg
     1861 cgggcggcca gacctactgg ggcggctggt cgtggtggtc gttcgactgc cgcgcccgca
     1921 ccgccgaccg gctggacttc gcctcggtcc gcgagggcgg cggcgaaggg cccgcgacgc
     1981 cggatacggc tccagcctat gaagcagcag ccggcggaga tgcggccgaa ctgctggcgg
     2041 tggcctgcga tccggcctcg gcgggcgagg cggacgccga caatctggcg gatgcggtca
     2101 ggatcgggcg agcggctctg gccggatagc ctgctccagc gccagccggt ccttcggcag
     2161 cgcttcgggg cattggtcac gcatgaaggc cagcatcttt tcgcggattt cgcagcgcag
     2221 gtcgaaggcc gtaggggcgt tgcgagcgct ggccaggcag cgaacctggg cgacgcggtc
     2281 ggtgatgtcg gtgacctgca gattgaccac atccccgtcc cagagcgggc tgtcgcggca
     2341 aatgcgttca agctccgccc gcagccggtc catcggcgcc tcgtagtcga cgtagagaaa
     2401 ggccgatccg atcaggctgg ccgtttcccg cgtccagttc tggaagggtt tggtgatgaa
     2461 gtaggtcagc ggcagaatca tccgccgcca gtcccaaagc ttgaccacca cataggtcga
     2521 ggtgatctc
//
LOCUS       DASVVN010000473         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_43769, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000473 DASVVN010000000
VERSION     DASVVN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43769"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF686_07605"
     CDS             <1..331
                     /locus_tag="VF686_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX8661718.1"
                     /translation="IGMHESTVSRVTSNKYVSTPRGVFELKFFFTAAIQSSDGGATHS
                     AEAVRHRIKSMIDGESRDGDVLSDDRIVEILKETGIDIARRTVAKYREALRIPSSVER
                     KRMLRTG"
     gene            530..1195
                     /gene="raiA"
                     /locus_tag="VF686_07610"
     CDS             530..1195
                     /gene="raiA"
                     /locus_tag="VF686_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX8661719.1"
                     /translation="MQIQVSGKHVDVGDALGSRISQELEDGIGKYFERGAQDAEVIVA
                     KEGHSFKVDCWVRLASGQVVFTSGLGGDAHSAFTESLEKLEKRVRRYKRRLKDHHIGP
                     KGLSPEKAENAAREVAQSIVLRDPDSVEDEDFGEAGAPDEPPPVGMVIAETEAEIRTI
                     TVGRAVLELDMTGYPVVLFRNAAHGGLSVVYRRPDGNVGWIDPERTAKSINGHGSVNG
                     KSG"
     gene            1271..1738
                     /gene="ptsN"
                     /locus_tag="VF686_07615"
     CDS             1271..1738
                     /gene="ptsN"
                     /locus_tag="VF686_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX8661720.1"
                     /translation="MDIGDLLADGGVVLRSGASSKRQALHTVAEAAAQALGLPENRVM
                     EALLEREALGSTGLGSGVAVPHARLEGVRQVTAVFVRLDTPVAYGAVDDRPVDLLFAL
                     FAPPRDGAEHLRALAAVSRALRSSEMREQLRQARTTDAIRALFVKDQAPATAA"
     gene            complement(1762..2016)
                     /locus_tag="VF686_07620"
     CDS             complement(1762..2016)
                     /locus_tag="VF686_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEX8661721.1"
                     /translation="MTPMMTKEDFRGLGSPDLVYVREIPAADVLADVIPSEAKGLTID
                     PHATLFAVHAADGERLAVMMDRETAFAAAVAHELEPVSVH"
     gene            complement(2100..2519)
                     /locus_tag="VF686_07625"
     CDS             complement(2100..2519)
                     /locus_tag="VF686_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX8661722.1"
                     /translation="MTTRTILFDSPFLLGFDHTRAMIDRAARAAADSYPPYNVEQRGE
                     HAVRITLAVAGFGPEDLAVTLEGRQLTIAGKREPSDKADQAFLHRGIAGRGFVRSFVL
                     ADGLEVEEARLEHGLLHVDMSRPEDLPTIRRIPITTG"
     gene            complement(2619..3044)
                     /locus_tag="VF686_07630"
     CDS             complement(2619..3044)
                     /locus_tag="VF686_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8661723.1"
                     /translation="MKTWIQLDGLKRGEDAAFLLLRLYLGGFLIWGVWDNIVSTARMA
                     EFQAFLTGLNCPLPALAAPVSVWAQLLIGVLLIPGLLTRWAGVLLAINFLVAVLLLGG
                     AGATVRDLFGPTMCVLAGLVLAAHGAGQWSVDARLLQQR"
     gene            complement(3152..3227)
                     /locus_tag="VF686_07635"
     tRNA            complement(3152..3227)
                     /locus_tag="VF686_07635"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Ala,seq:ggc)
     gene            complement(3384..>3722)
                     /locus_tag="VF686_07640"
     CDS             complement(3384..>3722)
                     /locus_tag="VF686_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX8661724.1"
                     /translation="KLRNRRGRPAGYAADTPEEDAEDQVEKKAAGEDEDEEEAVVTPE
                     IDEEGHEPILTPDDDDETPVDPTEESGMGEAVEDDDVLADDDDDSVPFIEDEDDDAFE
                     DEIITPKDDE"
BASE COUNT          604 a         1218 c         1262 g          638 t
ORIGIN      
        1 catcggcatg catgaatcca cggtcagccg ggtgacctcg aacaaatacg tctcgacccc
       61 gcgcggcgtg ttcgaactga aattcttctt cacggcggcc atccagtcgt ccgacggcgg
      121 cgcgacccat tcggcggaag ccgtccggca ccggatcaag tccatgatcg acggcgagag
      181 ccgcgacggc gacgtcctca gcgacgaccg gatcgtggag atcctcaagg agacgggcat
      241 cgacatcgcc cgccgcaccg tggcgaaata ccgcgaggcc ttacgtatcc cctcgtcggt
      301 cgagcgcaag cggatgctgc ggacgggctg aacgccgtcg ctgtctctcc tgtctctcat
      361 ttcccactcg cccgtcgtcg aattccttcg gattccgtca tcgaattcct cgtcgccgga
      421 atgatcgaat tctccggtgc gcgggtcgtc gaattcctgc agcccgaacc ttcaccacat
      481 ttcggtgatc ggcgttgaca ccattcccgt tcggtcgcgt tctatcggca tgcaaatcca
      541 agtcagcggc aagcacgtgg atgttggcga cgcgttaggc tcgcgcatct cccaggaact
      601 cgaagacggg atcggcaaat acttcgaacg aggggcccag gacgccgagg tcatcgtcgc
      661 caaggagggc cacagcttca aggtcgactg ctgggttcgt ctggcgtcag ggcaggtggt
      721 gttcacctcg ggactgggcg gcgacgccca ttcggccttc accgaaagtc ttgaaaagct
      781 cgagaagcgg gtccgccgct acaagcggcg gctcaaggac caccatatcg ggcccaaggg
      841 tctctcgccg gagaaggccg agaatgctgc ccgcgaagtg gcccagagca tcgttctgcg
      901 tgaccccgac agcgtcgagg acgaggattt cggtgaggct ggcgcgcctg acgagccgcc
      961 gcctgtgggc atggtcatcg cggagaccga ggcggaaatc cgcaccatca cggtgggtcg
     1021 cgcggtgctc gaactggata tgaccggtta tccggtcgtc ctgttccgca acgcggcgca
     1081 cggcggcctt tcggtggtct atcgccggcc ggacggcaat gtgggctgga tcgaccccga
     1141 gcggaccgcg aagtcgatca acggacacgg ttcggtaaac ggtaagagcg gatagtcttc
     1201 cccaatagag gcggttggtc taaacggccc ccgcccgttc gggatgtatg ggcagagtcg
     1261 ggaactcctc atggacatcg gtgatttgct ggcggacggc ggagtcgtcc tgcgcagcgg
     1321 tgcgtcgtcg aagcgtcagg ccctgcacac ggtggccgag gcggccgccc aggctctggg
     1381 cctgccggaa aaccgcgtca tggaagcgtt gctggagcgc gaggcgctgg gctcgaccgg
     1441 tctcggttcg ggcgtggccg tgccgcacgc gcggctggag ggcgtcaggc aggtgacggc
     1501 cgtgttcgtg cggctggata cgccggtggc ctatggcgcg gtcgacgatc gccctgtcga
     1561 tctgctgttc gccctgttcg ccccgccgcg cgacggcgcc gaacatctgc gcgcgctggc
     1621 ggcggtttcc cgcgccctca gatcatcgga aatgcgcgag cagctgcggc aggcccgcac
     1681 caccgacgcg atccgcgccc tgttcgtcaa ggatcaggct cccgccaccg cggcctgagg
     1741 cgcgcgtctg cttgggtgcg gtcagtggac cgacacgggc tccagctcgt gggccacggc
     1801 ggcggcgaag gcggtttcac ggtccatcat cacagccagc cgctctccgt cggcggcgtg
     1861 gacggcgaaa agggtggcgt gaggatcgat cgtcagaccc ttggcctccg aagggatcac
     1921 gtcggcgagc acgtcggccg cgggaatctc gcgcacatac accaggtcgg gcgaacccag
     1981 gccccggaag tcttctttgg tcatcatcgg cgtcatcgag accgcctcct tgtactgaac
     2041 taacgcccgt cgagggcgta tgggtccggc cccgggagcg gcgaccggaa aaaggccttt
     2101 cagcctgtcg tgatgggaat gcgccggatg gtcggcaggt cttcgggccg gctcatgtcg
     2161 acatgcagca ggccgtgctc cagccgcgcc tcctcgacct ccagcccgtc ggccaggacg
     2221 aagctgcgga cgaagccgcg accggcgatg ccgcggtgca ggaaggcctg gtcggccttg
     2281 tctgacggtt cccgcttgcc ggcgatggtc agctggcggc cctccagcgt cacggccaga
     2341 tcctcgggcc cgaagccggc gaccgccagg gtgatccgaa cggcgtgctc gccgcgctgt
     2401 tcgacattgt agggcggata gctgtcggcg gcggctcgcg cggcgcggtc gatcatcgcc
     2461 cgggtgtggt cgaagcccag caggaagggg ctgtcgaaca atatggttcg cgtcgtcatg
     2521 gtctcaggcc cccgaagcag gctgcggtcc gtctgcgccc cccgcgatcg gcgaggcgtc
     2581 ggcccgtgaa cgctagatgg ggcgcgtggc cccggtggtc aacgctgctg cagcagacgg
     2641 gcgtcgaccg accactggcc cgcgccgtgc gccgccagaa ccagcccggc aagcacgcac
     2701 atcgtgggac cgaacaggtc gcggacggtt gcgcctgctc cgcccagcag caggacggcg
     2761 acaaggaagt tgatcgctag cagcaccccc gcccagcgag tcagcagacc cgggatcagc
     2821 aggaccccga tcagcaactg ggcccagacc gagaccggcg cggccagcgc gggcagggga
     2881 cagttcaagc cggtcaggaa ggcctggaac tccgccatgc gggccgtcga gacgatattg
     2941 tcccacacgc cccatatcag aaagccgccc agatagagcc gcagcagcag gaaggcggcg
     3001 tcctcgccgc gcttcagacc gtcaagctgg atccaggtct tcatccgccg tctccctgcg
     3061 tgcagggcga aggctagctt gaccgtgggt acgtgggaag tgcggacccc ggatgggacg
     3121 aaggccccgc ggtttcccgc ggggccttcg atggtggagc ttagcggagt cgaaccgctg
     3181 acctcttgca tgccatgcaa gcgctctacc agctgagcta aagccccgaa ccttttgggt
     3241 cggatcgctt ggggccggga ggccctgctg cgaggcggcg gaacctatgt cagggtctga
     3301 tcgcgatcaa gcccggttca gccatttatt ttcgtcgccc ccggaccggt gaaaaccggt
     3361 ccgggaacgg gccgaaaaat cgtctattcg tcgtccttgg gcgtgatgat ttcgtcctcg
     3421 aacgcgtcgt cgtcctcgtc ctcgatgaag gggaccgagt cgtcatcgtc gtcagccagg
     3481 acgtcgtcgt cctcgaccgc ttcgcccatg ccggactctt cggtggggtc gacgggggtc
     3541 tcgtcgtcgt cgtccggcgt caggatcggc tcgtggcctt cctcgtcgat ctcgggcgtc
     3601 acaacggctt cttcctcgtc ctcgtcctca ccggcggcct tcttctcgac ctggtcctcg
     3661 gcgtcctctt cgggcgtgtc ggcggcgtag ccggccgggc ggccgcggcg gttgcgcagt
     3721 tt
//
LOCUS       DASVVN010000474         5508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_439646,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000474 DASVVN010000000
VERSION     DASVVN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439646"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2916
                     /gene="carB"
                     /locus_tag="VF686_07645"
     CDS             <1..2916
                     /gene="carB"
                     /locus_tag="VF686_07645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8661725.1"
                     /translation="SFTLAGTGGGIAYNVEEYREIVERGLDLSPTTEVLGEESVLGWK
                     EFEMEVVRDKADNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEYQRMRTGSINVL
                     REIGVETGGSNVQWAINPADGRMVVIEMNPRVSRSSALASKATGFPIAKVAARLAVGY
                     TLDELTNDITQVTPASFEPSIDYVVTKIPRFAFEKYPGAEATLSTSMKSVGEVMAIGR
                     TFQESMQKALRGLETGLTGFNEIEIDGVVDVEDEASARAAVVRALGQPTPDRILVIAQ
                     AFRHGLSVEEVNAACSYEPWFLRQIADIVREEGHIRVKGLPGLDPSTPSGSPSPSLRD
                     REETVGRPVSSPRSGEGDHEVVEGYRREFRRLKSKGFSDARLAQLTGTTEAAVRRARR
                     GLNVRPVFKRIDTCAAEFASATAYMYSTYETGALGQIPQCESDPTDKKKAIILGGGPN
                     RIGQGIEFDYCCCHAAFAFDDIGVESIMVNCNPETVSTDYDTSDRLYFEPLTAEDVLE
                     LIDVERSKGELIGVVVQFGGQTPLKLAHALQEDGVPILGTSVDSIDLAEDRERFQVML
                     HDIGLMQPPNGLARSAEEAAQKADEVGYPVVLRPSYVLGGRGMMIVHDREQLDRYVGE
                     AMRVSGDDPVLIDHYLNRATEVDVDALCDDETVFVAGVLEHIEEAGVHSGDSACSMPP
                     FSLRPEIVEELKRQTEAMARALKVRGLMNVQFAIEEPHSAEPRIFVLEVNPRASRTAP
                     FVAKTIGQPIAAIAAKVMAGVPLKSFGLADKPYDHIAVKEAVFPFARFAGVDTILGPE
                     MRSTGEVMGLDWKRDGEADMAPAFARAFAKSQIGGGTILPVAGCAFVSVKDGDKPFIV
                     EAVKTLLAEGFSIVATGGTHTFLTEQGLPVGHVKKVLEGRPNIVDAMKNGEVQLVFNT
                     TEGKQALLDSFSLRRTALMMKIPYYTTTAGALAAAQAIGAIKAGELDVRPIQDYAHD"
     gene            3243..3647
                     /locus_tag="VF686_07650"
     CDS             3243..3647
                     /locus_tag="VF686_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661726.1"
                     /translation="MEHYRLRKYRGPETWAQVRKAYVAGESAPSVARRFDVGLANLRR
                     RAMAEGWTRNRIAERLDLRPLRGGADDPPPALMALAELEAMPETPQIDPQAALRKAVR
                     RAAWLVSQGQAAEAGALLRAAEALDRMKWAAK"
     gene            complement(3686..4483)
                     /locus_tag="VF686_07655"
     CDS             complement(3686..4483)
                     /locus_tag="VF686_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661727.1"
                     /translation="MILLHGTLTALEDMMIGLAEPLALSHRVIAFDRPGFGQSTVRRF
                     ADAGIWRQAEAIHHATIALGLERPIVVGHSFGASVALAMAMDWPDSVAGVVALAPVVR
                     PEARLEHVLFGPRAAPVTGDWLSLFAGMSSDRMLFPLLWRSMYLPQSMPVRVEKEFPF
                     SMAGRSEAAARVGEDSMSAMPDLVRLNIRAPSCEVPVRIMGGDRDVVVRNGMNGRPLA
                     EVMPNAQFIDLPGLGHMVHHFATAEIVAVVEQLEALTPQVGRETNVA"
     gene            complement(4743..>5508)
                     /locus_tag="VF686_07660"
     CDS             complement(4743..>5508)
                     /locus_tag="VF686_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8661728.1"
                     /translation="AATRLSARLRQPLTVFFEAVAETMIVIVHWVLLAAPVGVFALSL
                     GVGLRAGLGAAGVLGHYIAIVSLALIGLILLTYVVAVAFGRVTLGRFARAAAPAQVVA
                     FSTQSSLACLPVMVERARDRLGVSAATAGLVLPLAVAVFRITSTVANLAVCIYVAHLY
                     GIPLTPGVLFAGGVAALAISVGTVGLPGQVSFFASIAPIALAMGLPLDVLPLLLAVEV
                     VPDIFRTVGNVTADLTAARIVEGRDATADPEAASGI"
BASE COUNT          960 a         1859 c         1887 g          802 t
ORIGIN      
        1 tcctttacgc tcgccggcac cggcggcggc atcgcctaca atgtcgagga ataccgcgag
       61 atcgtggagc gcgggctcga cctgtccccg accaccgagg tgctgggtga ggagagcgtg
      121 ctcggctgga aggagttcga gatggaggtg gtccgcgaca aggcggacaa ctgcatcatc
      181 gtctgctcca tcgagaacat cgacccgatg ggggtccaca ccggcgacag catcaccgtc
      241 gctccagccc tgacgctgac ggacaaagag taccagcgga tgcgcacggg ctcgatcaac
      301 gtcctgcgcg agatcggcgt cgagaccggc ggatccaacg tccagtgggc gatcaacccg
      361 gccgacggcc gcatggtcgt gatcgagatg aacccgcgcg tgtcgcgcag ctcggcgctc
      421 gcctcgaagg ccaccggctt cccgatcgcc aaggtcgcgg cgcgtctggc cgtcggctac
      481 accctggacg aactgaccaa cgacatcacc caggtcacgc cggcttcgtt cgagccgagc
      541 atcgactatg tagtcaccaa gatcccgcgc ttcgccttcg agaaataccc cggcgccgag
      601 gcgaccctgt cgacctcgat gaagtcggtg ggcgaggtca tggccatcgg ccggaccttc
      661 caggaatcga tgcagaaggc cctgcgcggc ctcgagaccg gcctgaccgg cttcaacgag
      721 atcgagatcg acggtgttgt cgacgtcgag gacgaggcct cggcccgcgc cgccgtggtg
      781 cgcgcgctgg gccagccgac cccggaccgg attctggtca tcgcccaggc cttccgccac
      841 ggcctgtccg tcgaggaggt caatgccgcc tgttcctatg agccgtggtt cctgcgccag
      901 atcgccgaca tcgtgcggga agaggggcat atccgggtga agggtttgcc cggccttgac
      961 ccctccaccc cttcggggtc cccctcccca tcgcttcgcg acagggagga gacggtgggc
     1021 cgtcctgtct cctccccacg cagtggggag ggggaccacg aagtggtgga ggggtatcgc
     1081 cgcgagttcc ggcgcctgaa gtccaagggc ttctcggacg cgcgtctggc ccagctgacc
     1141 ggcacgaccg aggccgccgt gcgccgggcc cgccgcggcc tgaacgtgcg ccccgtcttc
     1201 aaacgcatcg acacctgcgc cgccgagttc gcctcggcca cggcctatat gtattcgacc
     1261 tatgagaccg gcgcgctggg ccagattccg cagtgcgaat ctgatccgac cgacaagaag
     1321 aaggccatca ttctgggcgg cggtccgaac cggatcgggc aaggcattga gttcgattac
     1381 tgctgctgcc acgcggcttt cgccttcgac gacatcggcg tcgagtcaat catggtcaac
     1441 tgcaaccccg agactgtctc gaccgactac gacacctccg accgcctgta tttcgagccg
     1501 ctgacggccg aggacgtgct ggagctgatc gacgtcgagc gctcgaaggg cgagctgatc
     1561 ggcgtcgtgg tgcagttcgg cggccagacg ccgctgaagc tggcccatgc gctgcaggag
     1621 gacggggtgc cgatcctggg caccagcgtg gactccatcg atctggccga ggaccgcgag
     1681 cgcttccagg tcatgctgca cgacatcggc ctgatgcagc cgcccaacgg cctggcccgc
     1741 agcgccgagg aggccgcgca gaaggcggac gaggtcggct atcctgtcgt cctgcgcccc
     1801 tcatacgtgc tgggcggacg cggcatgatg atcgtccacg accgcgaaca gctggaccgc
     1861 tacgtcggcg aagccatgcg ggtctcgggc gacgaccccg tgctgatcga ccactatctg
     1921 aaccgcgcca ccgaggtgga cgtcgacgcc ctgtgcgacg acgagaccgt cttcgtcgct
     1981 ggcgtgctgg agcatatcga ggaggccggc gtgcattcgg gcgacagcgc ctgttcgatg
     2041 ccgccgttct cgctgcgtcc ggagatcgtc gaagagttga agcgccagac cgaggccatg
     2101 gcccgggcgc tgaaggtgcg cggcctgatg aacgtgcagt tcgccatcga ggagccgcac
     2161 agcgccgaac cgcgcatctt cgtgctggaa gtcaatccgc gcgccagccg cacggcgccc
     2221 ttcgtggcca agaccatcgg ccagccgatc gccgccatcg ccgccaaggt catggccggc
     2281 gtgcccctga aatccttcgg cctggcggac aagccctacg accatatcgc ggtcaaggag
     2341 gcggtcttcc cgttcgcccg cttcgccggg gtcgacacca tcctgggccc tgagatgcgt
     2401 tcgacgggcg aggtcatggg tctggactgg aagcgcgacg gcgaggccga catggccccc
     2461 gccttcgccc gcgccttcgc caagagccag atcggcggcg gcaccatcct gcccgtcgcc
     2521 ggctgcgcct tcgtgtcggt caaggacggc gacaagccct tcatcgtcga ggcggtgaag
     2581 accctgctgg ccgagggctt ctccatcgtc gccaccggcg gcacgcatac cttcctgacc
     2641 gaacagggcc tgcccgtcgg ccatgtgaag aaggtgctgg agggccggcc caacatcgtc
     2701 gacgcgatga agaacggcga ggtccagctg gtcttcaaca ccaccgaggg caagcaggcg
     2761 ctgctggaca gcttcagcct gcggcgcacg gccctgatga tgaagatccc ctattacacc
     2821 acaaccgcgg gcgcgctggc ggcggcgcag gcgatcgggg cgatcaaggc cggcgaactg
     2881 gatgtgcggc cgatccagga ctatgcgcac gactgaccag ctggcgacta ttttgaccat
     2941 gccgtgagcg caaaagcggg tgcagttcgg tcaggttggt tatgcgggtt tctgcaaaga
     3001 ctgctgaaag ccgccggtgc ggttcgcggc gtcccttcct gtcgtcatcc cggcgagggc
     3061 cgggacccag cggcgccgaa ggcgatctgt taacgcgtgc cggttggcga ccgggtccgc
     3121 gacagggtcg cgctctcgcg cgccgccggg tcccggggtc gctgcgcgcc ccgggatgac
     3181 gacggcggag gtccactccc tacacgtccg tttctgacgc acgcccgtgc caccatcagc
     3241 gcatggaaca ctatcgtctg cgcaaatatc gcgggcccga gacgtgggcg caggttcgaa
     3301 aggcctatgt cgccggagaa tccgccccgt ccgtcgcccg ccgcttcgac gtcggcctgg
     3361 ccaatctgcg ccgccgcgcc atggccgagg gctggacccg aaaccggatc gcggagcggc
     3421 tggacctcag gccgctgcgg ggcggggccg acgatccgcc gccagccttg atggcgctgg
     3481 cagagctgga ggcgatgccg gagacgccgc aaatcgaccc gcaggcggcg ctgaggaagg
     3541 cggtccggcg cgcagcctgg ctggtcagtc agggacaggc ggcggaggcc ggagcgttgc
     3601 tacgcgcggc cgaggcgctg gaccggatga aatgggcggc gaagtgaggc ccgtccaacc
     3661 atagaccagg ggccgctttg gacactcagg cgacattcgt ctcccggccc acctgcgggg
     3721 ttagcgcttc cagttgctcg acgacggcca cgatctcggc ggtcgcgaag tggtgaacca
     3781 tgtggccgag gccaggcaga tcaatgaact gcgcattggg catgacctca gccagcggcc
     3841 ttccattcat gccgttgcgc acaacaacgt cccggtctcc gcccatgatg cgcacgggaa
     3901 cctcgcagga tggcgcgcgg atattgagcc tcacgaggtc gggcatcgcc gacattgagt
     3961 cctcgccgac gcgagccgcg gcctcacttc gacccgccat ggagaaggga aactccttct
     4021 cgacccggac cggcatggat tgcgggaggt acatcgagcg ccaaagcaac gggaacagca
     4081 tccggtcgct gctcattccg gcaaacaggc tgagccaatc gccggtgact ggggcggcgc
     4141 gagggccgaa caggacgtgc tccagcctcg cctcaggccg gacgaccggc gcaagcgcca
     4201 cgacccctgc aacgctgtcc ggccagtcca ttgccatcgc cagggcgacg gaagcgccga
     4261 acgaatgacc aacaacaatc ggccgctcaa ggcccagcgc gatcgtggcg tggtggatgg
     4321 cttcggcctg tctccagatg cccgcatccg cgaaccggcg tacagtgctc tgtccaaaac
     4381 ctggacgatc gaaagctatc actcgatggc tcagggccag aggctcggcg aggccgatca
     4441 tcatgtcttc aagtgctgtc agcgtcccat gcagcaggat cactggcggt ccctggccgg
     4501 cttcagcgta agccagcgaa agaccgtccc gcgtgggagc gaaccgccgc ttcagaaccg
     4561 tcccggcgcg gtcaagcagg ttggcgggcg gggatcggtg cgattgaggc atgatggtcg
     4621 ggctagcgct tgacggtcat cctcggttcc agcccctcgc cggaaggcgg gcatggaact
     4681 ccgcgacccc tcgcgatatc gccgtctgag agattgaacc cgggcggcga ctgcttccgg
     4741 cctcagattc ccgacgccgc ctccgggtcc gccgtcgcgt cccttccctc gacgatgcgc
     4801 gccgccgtca ggtcggcggt gacattgccg acggtgcgga agatgtccgg cacgacctcg
     4861 accgccagca gcagcggcag gacgtccagc ggcaggccca tggccagggc gatgggggcg
     4921 atggaggcga agaaggacac ctgacccggc aggcccacgg tgccgacgct gatggccagg
     4981 gcggccacgc cgccggcgaa caggacgccg ggggtcaggg ggatgccgta caggtgggcg
     5041 acgtagatgc agacggcgag gttggcgacg gtcgaggtga tgcggaacac cgccaccgcc
     5101 agcggcagaa ccagaccggc ggtcgcggcg gagacgccca gccggtcccg ggcgcgttcg
     5161 accatcaccg gcaggcaggc cagcgaggac tgggtcgaga aggccacgac ctgggccggg
     5221 gcggcggcgc gggcgaagcg gccgagcgtg acccggccga aggcgacggc caccacatag
     5281 gtcagcagga tcaggccgat cagggcgagg gagacgatgg cgatgtagtg gcccagcacc
     5341 cctgccgcgc ccagacccgc tctcaggccc acacccagcg acagggcgaa gacgccgacc
     5401 ggcgcggcca gcagcaccca gtggacgatg acgatcatgg tctcggccac cgcctcgaag
     5461 aagacggtca gcggctggcg cagccgcgcc gacaggcgcg tggccgcg
//
LOCUS       DASVVN010000475         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_332900,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000475 DASVVN010000000
VERSION     DASVVN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332900"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..356
                     /locus_tag="VF686_07665"
     CDS             81..356
                     /locus_tag="VF686_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor I78"
                     /protein_id="HEX8661729.1"
                     /translation="MRARVAAAAAGLTLAACAPVDTPGTSPVPQRCDAAAAQSLVGSH
                     VGAVDFAAGANVRIVCTTCPTTKDLRPDRLNVRFNQTTGIIERVDCG"
     gene            417..2117
                     /locus_tag="VF686_07670"
     CDS             417..2117
                     /locus_tag="VF686_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661730.1"
                     /translation="MRTILMAGVALGAALIGPAAVADATADSAVRALLERPQVCGQAG
                     SANSGTPVADMVMVDGFGTGGFTIDTASPEAQAWFNHGVRLRWAFEHKESVRAFRKAR
                     LLDPTCAMCAWGEAWALGPNLNGGGNDDESLAAALKAAREARRMARQATPMQRQMIDA
                     LVQRYSSWKSSRSRRFAQSMDRIARNNPTDVTVAAITADAWMLNADEWWDDDGKAADP
                     GITRAMAILEQTLAHAPDDPGAIHLYIHLTEWSDDPHKAIPYGERLAALAPGASHLVH
                     MPSHTFYRVGRYKDAMMSNVNAVALDRRYDGLARPPGGVPGMPLHGHNIHFGMGGALM
                     AGGGEEGLNLADWFLATYPDIPATNVWRQAMANNAYAIYGRFGSPEKVASLTEPAESH
                     PMLRVSWRFARGEAAARAGDASAVRVEAEAIAALRAAPDFASGPQGKMRNDFTEVSQR
                     VLEGRAAMIEGNADAAIAAFTRAAEIQASGDSEGGDPPILWYPTRRSLAAALLLKGDA
                     AAAKAKIVELLEDWPGDPYSYFVLAEAETALGDAAAAAEARRKAAVEWIGGPMDLKLA
                     "
     gene            complement(2125..2997)
                     /gene="panC"
                     /locus_tag="VF686_07675"
     CDS             complement(2125..2997)
                     /gene="panC"
                     /locus_tag="VF686_07675"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX8661731.1"
                     /translation="MTDPASLPVVRTVAALREQVSAWKRQGFTVGFVPTMGALHEGHL
                     TLVREAGRRADRVVASVFVNPTQFAAHEDLATYPRREAQDAEMLAGAGCHLLFAPTVG
                     EMYPEGFATAVSVGGPSAGLETDFRPHFFGGVAVVVAKLLNQVQADVAVFGEKDYQQL
                     MVVRRMARDLDIPTDIVGSPTERDGHGLALSSRNAYLNEAELETARRLNGVLAEAGVQ
                     ATARRPLAAVERDAHAALLKAGFERIDYVAVRRADDLAPFRDGVVDSPARILAAAWLG
                     RTRLIDNMAVAAPT"
BASE COUNT          472 a         1081 c         1028 g          421 t
ORIGIN      
        1 gtgacgatgg actacagccc gtcgcgcttg aatatcttct acgaggagcg gacggggatc
       61 atccgcgagg tcaaatgcgg atgagggcgc gcgtcgccgc cgctgccgcc ggcctgacgc
      121 tggcggcgtg cgccccggtc gatacgcctg ggacctcgcc cgtgccccag cggtgcgacg
      181 ccgcggcggc gcagtcgctg gtcggctctc acgtcggggc agtggatttc gcggccggag
      241 ccaacgtccg catcgtctgc accacctgtc cgacgacaaa ggacctgcgt ccagaccggc
      301 tgaacgtccg cttcaaccag acgaccggca tcatcgaacg ggtcgactgc ggctgatccc
      361 gcttggcgtc cccagccttt cacggcaggg tggcgtcgag ggacagggag acggctatgc
      421 gcacgatatt gatggcgggc gtcgcgctgg gcgcggcgtt gatcgggccc gcggccgtcg
      481 ccgacgcgac cgccgacagc gccgtgcggg ccctgctgga gcggccccag gtctgcggac
      541 aggccggctc ggccaactcc ggcacgcctg tcgccgacat ggtcatggtc gacggctttg
      601 gcacgggcgg cttcaccatc gatacggcca gtcccgaagc ccaggcctgg ttcaaccacg
      661 gcgtccgtct gcgttgggcg ttcgagcaca aggaatcggt gcgcgccttc cgcaaggcgc
      721 gcctgctgga cccgacatgc gccatgtgcg cctggggcga ggcctgggct ctgggcccga
      781 atctgaacgg cggcggcaat gacgacgaaa gcctcgccgc cgccctgaag gccgcgcgcg
      841 aggcccgacg gatggcccgg caggcgacgc ccatgcagcg gcagatgatc gacgcgcttg
      901 tgcagcgcta ttccagctgg aaatcctcgc gcagtcgccg tttcgcccag tccatggacc
      961 ggatcgcacg caacaatccg acggacgtga cggtggcggc catcaccgcc gacgcctgga
     1021 tgctgaacgc cgacgaatgg tgggacgacg acggcaaggc cgcggatccc ggcatcaccc
     1081 gggccatggc catcctcgaa cagaccctgg cccacgcccc cgacgaccca ggcgccattc
     1141 acctctacat ccacctgacc gaatggtccg acgatccgca caaggccatt ccctatggcg
     1201 agcggctggc ggccctggcc cccggggcca gccatctggt gcatatgccg tcgcacacct
     1261 tctaccgggt cggccggtac aaggacgcca tgatgtccaa cgtcaacgcc gtggcgctgg
     1321 accggcgcta cgacgggctg gcccgtccgc ccggcggcgt gcccggcatg ccgctgcatg
     1381 gccacaacat ccacttcggc atgggcggcg cgctcatggc cggcggcggc gaggaagggc
     1441 tgaacctggc tgactggttc ctcgccacct atccggacat tccggcgacc aatgtctggc
     1501 ggcaggccat ggccaacaac gcctacgcca tctacggccg cttcggctcg ccggagaagg
     1561 tcgcctccct gaccgagccg gccgagagcc atccgatgct gcgcgtcagc tggcgcttcg
     1621 cccggggcga ggcggcggcg cgggcagggg acgcctccgc cgttcgggtc gaggccgagg
     1681 ccatcgccgc cctgcgcgcc gcgccggact tcgcctccgg gccgcagggc aagatgcgca
     1741 atgacttcac cgaggtttcg caacgggtgc tcgaaggccg cgccgccatg atcgagggca
     1801 atgccgacgc cgccatcgcg gccttcacgc gcgcggctga aattcaggcc agtggtgatt
     1861 ccgagggcgg cgacccgccg atcctctggt atccgacccg ccgcagcctc gccgcggccc
     1921 tgctgctcaa gggtgacgcg gcggccgcaa aggccaaaat cgtcgaactg ctcgaggact
     1981 ggcccggcga cccgtacagc tatttcgtcc tcgccgaggc ggaaacggcg ctgggcgacg
     2041 cggccgcggc ggccgaggcg cgccgcaagg cggcggtcga atggatcggc ggaccgatgg
     2101 acctgaagct ggcctaggcg gccctcaggt cggggccgcg accgccatat tgtcgatcag
     2161 ccgtgtccgg cccagccagg ccgccgccag aatgcgcgcc ggcgagtcca cgactccgtc
     2221 gcggaacggc gccagatcgt cggcgcggcg tacggcgaca tagtcgatgc gctcgaaccc
     2281 ggctttcagc agggccgcgt gggcgtcgcg ttcgacggcc gccagcggac gccgcgctgt
     2341 cgcctgcacc ccggcctcgg ccagcacccc gttcagccgc cgcgcggtct ccagctcggc
     2401 ctcgttcaga taggcgttgc gcgacgacag ggccaggccg tggccatcgc gttcggtcgg
     2461 cgagccgacg atgtcggtgg ggatatccag atcgcgcgcc atgcgccgca cgaccatcag
     2521 ctgctgatag tccttctctc cgaacacggc gacgtcggcc tggacctgat tgagcagctt
     2581 ggcgaccaca acagccaccc cgccgaagaa gtgcgggcgg aagtcggtct ccagtcccgc
     2641 cgacggcccg ccgacgctga cggcggtggc gaagccttcc ggatacatct ccccgaccgt
     2701 cggcgcgaac agcagatggc agcccgcgcc ggccagcatt tccgcgtcct gcgcctcgcg
     2761 ccgggggtat gtggccaggt cctcatgcgc cgcgaactgg gtcggattga cgaagacgct
     2821 ggcgaccacg cggtccgcac gccgcccggc ttcccgcacc aaggtcaagt gaccttcgtg
     2881 caacgctccc atggtcggaa cgaagccgac cgtgaacccc tggcgcttcc acgcgctcac
     2941 ctgttcgcgc aacgcggcga cggtgcggac gacgggaaga gacgcgggat cggtcatgat
     3001 ga
//
LOCUS       DASVVN010000476         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_332963,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000476 DASVVN010000000
VERSION     DASVVN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332963"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VF686_07680"
     CDS             complement(<1..960)
                     /locus_tag="VF686_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8661732.1"
                     /translation="MRDINHFIDGASFTGASGRFSDVFNPNTGEVQARVQLASVAEMD
                     RAVQAAQNAFEGWSSTNPQRRARVMFEFKRLVEANMNELAELLSSEHGKVVADSKGDI
                     QRGLEVIEFACGIPHALKGEYTWGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMF
                     GVAIAVGNTFILKPSERDPSVPVRLAELMMEAGAPAGVLNVVHGDKAAVDAILTHPLI
                     HAVSFVGSSDIAHYVYQTGAANHKRVQAMGGAKNHGIVLPDADMDQTVKDLLGAAYGS
                     AGERCMAISVAVLVGDVADRIVPKLAERARSLKISNGMELDAEM"
     gene            complement(1043..1933)
                     /locus_tag="VF686_07685"
     CDS             complement(1043..1933)
                     /locus_tag="VF686_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661733.1"
                     /translation="MDAIRLSPPRRLSLPVSNRWGEGRMSVLDFGDAQRPVDIVFVHA
                     NGFNAMTYRTLLAPLSGSLRIWAPDLRGHGGTTLPTQIEGRRHWLDHRDDLIAVLDAI
                     DGPPVVLAGHSMGGTSALLAAAERPDRVAGLVLLDPVIWGRLTVAAFKLPLLDRLASR
                     IPLVRNALRRRAVFDSREQALTAYAGRGAFKGWPEMMLADYLADGLVETPAGFVLACD
                     PAWEASNYASQSHDPWRALKRVNRPVRILKGEHGSTCYLTGPPRGLPHVTVESVPGGT
                     HFFPMLRADIARDALFDAAV"
     gene            complement(1988..2470)
                     /locus_tag="VF686_07690"
     CDS             complement(1988..2470)
                     /locus_tag="VF686_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX8661734.1"
                     /translation="MSEHCHHHHAEGPPAAADVTRAMGEADRRLTAEGERMTPARLRV
                     LELLLAAGEPVKAYDLIARFGDDGQPAKPPTVYRALEFLERKGLAHRIASISAYVACT
                     SGSVDHAAAFLICDCCGATQEVSAPVAGDLGRAAEAAGYAIARTTIEAHGRCPACAAG
                     "
     gene            2579..>3155
                     /locus_tag="VF686_07695"
     CDS             2579..>3155
                     /locus_tag="VF686_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEX8661735.1"
                     /translation="MSLSPVSRAALTAALAAPLGWGVMAGAVAAQTATPPQEPARLDD
                     VIVTGAPFGISARATTIATTVIDEEALATAPAASLGDLVSGLPGMRSTDFAPGASRPV
                     IRGLSGPRVQVLTNGIGLIDASSVSPDHQVATDPAEANRIEIVRGPATLAYGGTAIGG
                     VVNVLDNRIPTEMPEGVDGLVSAQTSSVDDGW"
BASE COUNT          458 a         1067 c         1168 g          462 t
ORIGIN      
        1 catctcggcg tcgagctcca tgccgttgct gatcttgagc gagcgcgcgc gctcggccag
       61 cttgggcacg atgcggtcgg cgacgtcgcc caccagcacc gcgacgctga tcgccatgca
      121 gcgctcgccg gccgagccgt aggccgcgcc gagcaggtcc ttcactgtct ggtccatgtc
      181 ggcgtcgggc aggacgatgc cgtggttctt cgccccgccc atggcctgca cccgcttatg
      241 gttggcggcg ccggtctggt agacgtagtg ggcgatgtcg gacgagccaa cgaagctgac
      301 ggcatggatc agcgggtggg tcaggatggc gtcgaccgcc gccttgtcgc cgtgcacgac
      361 gttcagcacg ccggcgggcg cccccgcctc catcatcagc tcggccagac gcaccggcac
      421 cgacggatcg cgctcggacg gcttgaggat gaaggtgttg ccgacggcga tggccacccc
      481 gaacatccac atcgggatca tggccgggaa gttgaacggg gtgatgcccg cgaccacgcc
      541 cagcggctgg cgcatcgaat agacgtcgat gccgggaccg gcgccccagg tgtattcgcc
      601 cttcagcgca tgcgggatgc cgcaggcgaa ctcgatgacc tcaagcccgc gctggatgtc
      661 gcctttcgag tcggcgacga ccttgccgtg ctcgctgctg agcagttcgg ccagttcgtt
      721 catattggcc tcgacgagac gtttgaactc gaacatcacc cgcgcgcggc gctgggggtt
      781 tgtcgacgac cagccttcga aggcgttctg ggcggcctgg acggcgcggt ccatctcggc
      841 gaccgaggcc agctgcacgc gggcctgaac ctcgccggtg ttcggattga agacgtcgga
      901 gaaccggccc gaggcgcccg tgaagctggc gccgtcgatg aagtggttga tgtcgcgcat
      961 ggtcggaacg gtcctggaaa acgaaacagt ctgtacggat gggcagcggt ttagcccctg
     1021 acccgcgcgg gcgccagcgt gactagacgg cggcgtcgaa cagggcgtcg cgggcgatgt
     1081 cggcgcgcag catggggaag aaatgggtcc cgcccgggac ggactcgacg gtcacgtgag
     1141 gcaggccgcg cgggggccct gtcaggtagc aggtcgagcc gtgctcccct ttcagaatgc
     1201 ggacgggccg gttcacccgt ttcagggcgc gccaggggtc gtggctctgg gatgcgtagt
     1261 tggaagcctc ccacgcgggg tcgcaggcga ggacgaagcc ggcgggcgtc tcgacgagcc
     1321 cgtcggccag atagtcggcc aacatcatct cgggccagcc cttgaaggcc ccgcggccgg
     1381 cataggcggt cagcgcctgt tcccggctgt cgaagacggc gcggcggcgc agggcgttgc
     1441 gcaccagcgg gatgcgcgag gccagccggt cgaggagggg cagcttgaag gcggcgaccg
     1501 tcaatcggcc ccagatcact ggatccagca ggaccagacc ggcgacccgg tccgggcgtt
     1561 cggcggcggc cagcagcgcc gaggttccgc ccatggagtg gccggcgagg acgaccggcg
     1621 gtccgtcgat ggcgtccagc acggcgatca ggtcgtcgcg gtggtccagc cagtgccggc
     1681 ggccctcgat ctgcgtcggc agggtggtcc cgccgtggcc gcgcaggtcc ggcgcccaga
     1741 tgcggaggct gcccgacagg ggcgccagca gggtgcggta ggtcatggcg ttgaagccgt
     1801 tggcatggac gaagacgatg tcgaccgggc gttgggcgtc gccgaaatcc agcacgctca
     1861 tgcggccctc gccccagcgg ttcgacaccg gaaggctcag gcgacggggc ggggagagcc
     1921 ggatcgcgtc cattcagggc agataggcga tcgcggaggc ccgcgccagt tcacgcgggg
     1981 taaggcttca gcccgcggcg caggccggac agcggccgtg ggcctcgatc gtggtgcggg
     2041 cgatggcgta gccggcggcc tcggcggcgc ggcccagatc gccggcgacg ggggcggaca
     2101 cctcctgcgt cgcgccgcag cagtcgcaga tcaggaaggc cgccgcgtgg tcgacgctgc
     2161 ccgaggtgca ggcgacatag gcgctgatcg aggcgatgcg gtgggccagt cccttgcgtt
     2221 cgaggaactc cagcgcgcga tagacggtcg gcggcttggc cggctggccg tcgtcgccga
     2281 agcgggcgat caggtcatag gccttcaccg gctccccggc ggccagtagc agttccagca
     2341 cgcgcaggcg ggcgggagtc atccgctcgc cctcggccgt caggcggcgg tcggcctcgc
     2401 ccatggcgcg ggtgacgtcg gcggcggccg gcggtccctc ggcgtgatgg tgatggcagt
     2461 gctcgctcat gcgccagagc taagccttcg gtgcgggcgc cgcaaccgga gccgcttgac
     2521 ggcgtgtgat gttatctcgt aacaccgagt catcgccgtt ccagttccgg atgccttcat
     2581 gtccctctcg cccgtatccc gcgccgccct gaccgccgcc ctcgccgccc ctctgggctg
     2641 gggcgtgatg gccggagccg tcgccgccca gaccgcgacg cccccgcagg agccggccag
     2701 gctggacgac gtcatcgtca cgggcgcgcc gttcgggatc agcgcgcggg cgacgaccat
     2761 cgccaccact gtgatcgacg aggaggcgct ggccaccgcg cccgccgcct cgctgggcga
     2821 tctggtctcc ggcctgccgg gcatgcgctc gaccgatttc gcgcccggcg ccagccgccc
     2881 cgtgatccgg ggcctgtcgg ggccgcgcgt gcaggtgctg accaacggca tcggcctgat
     2941 cgacgccagt tcggtgtcgc ccgaccacca ggtcgcgacc gacccggccg aggccaatcg
     3001 catcgagatc gtgcgcggtc cggccaccct ggcctacggc ggcacggcca tcggcggcgt
     3061 cgtcaacgtg ctcgacaacc gcattccgac ggaaatgccc gaaggcgtgg acgggctggt
     3121 gtcggcccag acctccagcg tcgacgacgg ctggt
//
LOCUS       DASVVN010000477         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_439695,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000477 DASVVN010000000
VERSION     DASVVN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439695"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF686_07700"
     CDS             <1..372
                     /locus_tag="VF686_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8661736.1"
                     /translation="TNQVEYHPYLKLSKLKAAAKELGSSITAWSPLAQGQIADDATLI
                     EIGKAHGKTPGQVTLRWLIQQDIVAIPRTAKESRAAENFDIFDFQLSSEEMDRIHALA
                     RPDGRLGDWLDKAFQWDEEWA"
     gene            445..693
                     /locus_tag="VF686_07705"
     CDS             445..693
                     /locus_tag="VF686_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8661737.1"
                     /translation="MGSVLGFPGVDPLDAAVGARLKRWRETRGLSIEDVAAMIHMSAE
                     ETRRAEAGRAHLTTAQIGAATSALHLPLWALVSDTRAY"
     gene            693..1346
                     /locus_tag="VF686_07710"
     CDS             693..1346
                     /locus_tag="VF686_07710"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEX8661738.1"
                     /translation="MDRPRAARIWRAVFATVGWAALALQYGLMITGNPGTSPAELTLN
                     FFSFFTILTNVLVALALTLPVIGAGTRLGRRAGAEGVRAGVTMYAVVVGLVYHFLLHA
                     TWAPQGWSLVANALLHYVMPAAILLDWLLFTPRGRLCWIDAPKWLVFPLVYGGWTLIH
                     GSLAGWWPYWFLNIDELGLATAALYFMGLLVFFLVVGLGVVAIDRTLGRRDRTPASA"
     gene            1375..>1761
                     /locus_tag="VF686_07715"
     CDS             1375..>1761
                     /locus_tag="VF686_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX8661739.1"
                     /translation="MAYKSLRDFTAMLEAEGELVRVSEPVSTHLEMTEIQTRLLRNGG
                     PAILFEQPVMPDGSISPIPCLANLFGTVKRVAMGVTMEKKTRTTAAELREVGELLAFL
                     RNPTPPRGLGDAMEMLPLAQTVMSMRP"
BASE COUNT          271 a          632 c          567 g          291 t
ORIGIN      
        1 accaaccagg tcgaatacca cccctacctc aagctctcga agctgaaggc cgccgcgaaa
       61 gaactgggct cctcgatcac cgcatggtcg ccgctggcgc agggccagat cgccgacgac
      121 gcgaccctga tcgagatcgg caaggcccac ggcaagacgc ccggtcaggt caccctgcgc
      181 tggctgatcc agcaggacat cgtcgccatc ccgcgcaccg ccaaagagag ccgggcggcc
      241 gaaaacttcg acatcttcga cttccagctc tcgtccgagg agatggaccg catccacgcc
      301 ctcgcccgcc ccgacggccg tctgggcgac tggctcgaca aggctttcca atgggatgaa
      361 gaatgggcct gagacgcggc ggctgagacc gcgtccaatg cgctcaggca ggcgatagcc
      421 ggtagcgcaa aaggaggacg tcccatgggc tcggtgctgg gttttcccgg cgtcgatccg
      481 ctcgacgcgg cggtcggcgc gaggctgaag cggtggcgcg agacgcgcgg cctgtcgatc
      541 gaggacgtcg ccgccatgat ccacatgtcc gctgaggaaa cgcgccgggc cgaggctggt
      601 cgcgcccatc tgaccaccgc ccagattgga gccgccacca gcgccctgca cctgccgctg
      661 tgggcgctcg tctccgacac ccgggcctac tgatggatcg gccgcgcgcc gcccggatct
      721 ggcgcgcggt cttcgccacg gtcggctggg cggcgctggc gctgcagtat ggactgatga
      781 tcaccggcaa tcccggcaca agcccggccg agctgacgct gaacttcttc agcttcttca
      841 ccatcctgac caatgtgctg gtcgccctgg ccctgaccct gccggtcatc ggggccggga
      901 cccggctcgg ccgccgcgcc ggagcggagg gcgtgcgggc cggcgtgacc atgtacgccg
      961 tcgttgtcgg cctcgtgtac cacttcctgc tgcacgcgac gtgggccccg cagggctggt
     1021 cgctggtcgc caacgccctg ctgcactacg tcatgcccgc cgccatcctg ctcgactggc
     1081 tgctgttcac gcccaggggt cggctctgct ggatcgacgc gccgaaatgg ctggtctttc
     1141 cgctggtcta cggcggctgg acgctgatcc acggctccct tgccggctgg tggccatact
     1201 ggttcctgaa catcgacgaa ctcggcctcg ccaccgcggc gctctacttc atgggtctgc
     1261 tggtgttctt ccttgtggtc ggccttgggg tcgtggcgat cgaccgcacg ctgggacggc
     1321 gtgacaggac gcccgcgtcc gcctaagccg gacgctctga cggagcgttc tgccttggcc
     1381 tacaaatccc tgcgcgactt cacggccatg ctggaggccg agggcgaact ggttcgcgtc
     1441 tccgaacccg tctccaccca ccttgagatg accgagatcc agacccggct gctgcgcaac
     1501 ggcggccccg cgatcctgtt cgaacagccc gtcatgcccg acggctcgat cagccccatc
     1561 ccctgtctgg ccaatctgtt cggcacggtg aagcgcgtgg ccatgggcgt gaccatggag
     1621 aagaagaccc gcaccaccgc cgccgaactg cgcgaggtcg gcgaacttct ggccttcctg
     1681 cgcaacccga cccctccgcg tgggctcggc gacgccatgg agatgctgcc gctggcccag
     1741 acggtgatgt cgatgcggcc c
//
LOCUS       DASVVN010000478         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_254683,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000478 DASVVN010000000
VERSION     DASVVN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254683"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VF686_07720"
     CDS             complement(<1..996)
                     /locus_tag="VF686_07720"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX8661740.1"
                     /translation="MPDLDLALRLSAPESTLAVGGLVLLMLGAFMGEKSAKLVSALSV
                     ALLVAATAVTVTGPLGQAFNGAYVADPLAVYGKAVIFLSSAVAIILGNGWMHRARIAR
                     FEYPVLIVLASAGMGMMASSGDLISLYIGIELHSLALYVLAAFHRDDLKASEAGLKYF
                     VLGALSSGLLLYGASLIYGFAGSMRFDEIAAYAATNPSTGLIFGLVFLICGLAFKVSA
                     APFHMWTPDVYEGAPTPVVALFATAPKVAAMVLIARTLEGAFAGSHDQWAQVLILISL
                     ISFAVGAFGGLAQKDIQRLLAYSSIANIGYALLGIAAGTTLGIQAMLVFMTLYVID"
     gene            complement(1004..>1956)
                     /locus_tag="VF686_07725"
     CDS             complement(1004..>1956)
                     /locus_tag="VF686_07725"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517389.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX8661741.1"
                     /translation="LMLLAMLWMAAETGTTSIPALKDYAFSPTVQPLLWLAFFASFAV
                     KMPMWPVHTWLPDAHVQAPTAGSVILAGILLKLGGYGFILFNLPMFPDASKMFAPLVF
                     VLSAVAIVYTSLVAFRQTDMKKLIAYSSVAHMGFVTMGIFAGNALGVQGAVFQMLSHG
                     LISGALFLCVGVVYDRMHTREIALYGGLTARMPWFAAIFLLFTMANVGLPGTSGFIGE
                     ILTMTGVYGVSTWTALFAASGVIFSAVYALTLYRNVMFGEITNPAMKAITDVDRRELL
                     IFAPLIIGTLWLGVQPGLVLDYTAASVEALTSAYQLAIGG"
BASE COUNT          403 a          649 c          648 g          256 t
ORIGIN      
        1 gtcgatgacg tacagggtca tgaacaccag catggcctgg atgcccaggg tcgtgcccgc
       61 ggcgatgccc agcagggcgt agccgatgtt agcgatggac gaataggcga gcagccgctg
      121 gatgtccttc tgcgccagac cgccgaacgc gccgacggcg aacgagatca gcgagatcag
      181 gatcaacacc tgcgcccact ggtcgtgcga gcccgcaaag gcgccttcca gcgtgcgggc
      241 gatcagcacc atggccgcca ctttcggtgc ggtggcgaac agggccacca ccggcgtcgg
      301 cgcgccctcg tagacgtccg gcgtccacat gtggaacggc gccgccgaga ccttgaaggc
      361 caggccgcag atcaggaaca ccagaccgaa gatcaggccg gtcgacggat tggtcgcggc
      421 gtatgcggcg atctcgtcga accgcatcga gccggcgaag ccgtagatca ggctcgcgcc
      481 gtacagcagc aggcccgacg acagggcgcc cagcacgaaa tacttcagcc ccgcctcgga
      541 cgccttcagg tcgtcgcgat ggaaggcggc cagcacatac agggccagcg aatgcagctc
      601 gatgccgatg tagagcgaga tcaggtcgcc cgacgacgcc atcatcccca tgccggccga
      661 ggccagcacg atcagtaccg gatattcgaa ccgcgcgatc cgcgcccggt gcatccagcc
      721 gttgcccaga atgatggcca cggcgctgga caggaagatc acggccttgc cgtagacggc
      781 cagcggatcg gccacatagg cgccgttgaa cgcctgcccc agcgggccgg tcacggtcac
      841 tgcggtggcg gcgaccagca gggccaccga cagggccgac accagtttcg cgctcttctc
      901 gcccatgaag gcgccgagca tcagcagcac cagaccgccg acggccagcg tgctctcggg
      961 ggcggacagg cgcagcgcta gatcaagatc aggcatcatg gtctcacccg ccgatcgcaa
     1021 gttgataggc ggaagtcagg gcctcgaccg aggcggcggt gtaatccagc accagaccgg
     1081 gctgcacgcc cagccacagg gtgccgatga tcagcggcgc gaagatcagc aactcgcgcc
     1141 tgtcaacgtc ggtgatggcc ttcatcgcgg gattggtgat ctcgccgaac atgacgttgc
     1201 ggtacagcgt cagggcgtag accgccgaga agatcacgcc cgacgccgcg aacagcgccg
     1261 tccaggtcga gacgccgtag acgcccgtca tggtcaggat ctcgccgatg aagcccgagg
     1321 tgcccggcag gccgacattg gccatggtga acagcaggaa gatggccgcg aaccacggca
     1381 tccgcgcagt cagaccgccg tacagggcga tctcgcgggt gtgcatccgg tcgtagacca
     1441 cgcccacgca gaggaagagc gcgcccgaga tcaggccgtg gctcagcatc tggaacaccg
     1501 ccccctgcac gcccagggcg ttgccggcga agatgcccat ggtcacgaag cccatgtgcg
     1561 ccaccgacga ataggcgatc agcttcttca tgtccgtctg ccggaaggcg accagcgagg
     1621 tatagacgat ggccacggcc gacagcacga acaccagcgg cgcgaacatc ttcgacgcgt
     1681 cggggaacat gggcaggttg aacaggatga agccgtagcc ccccagcttc agcaggatgc
     1741 ccgccagaat gaccgacccc gccgtcggcg cctgcacgtg ggcgtccgga agccaggtgt
     1801 gaaccggcca catcggcatc ttgacggcga acgaggcgaa gaaggccagc cacagcaggg
     1861 gctgcaccgt cgggctgaag gcgtagtcct tgagcgccgg tatgctggtg gtcccggtct
     1921 cggcggccat ccacagcatg gccagcagca tcagca
//
LOCUS       DASVVN010000479         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_95866, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000479 DASVVN010000000
VERSION     DASVVN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95866"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VF686_07730"
     CDS             <1..1114
                     /locus_tag="VF686_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8661742.1"
                     /translation="AGFEGWKQGFLSRHGGVRRDQYARELAGLTPDPSVIRLDGNQPE
                     FSRPAGAYIQSAASATRIAQGRQRTERVPWEVTQRFGVPAEILVSIWGNESSFGAVQG
                     DYDVIRSLATLAFDGRRRDWAEAQLKDALDIVVDGRRDRAGLKGSWAGAMGQTQFMPD
                     NFLRLGVDQTGDGKVDIWGSDADSLASAANLLAQAGWKRGQSWGYEIVLPSGFDYAEA
                     EGPRHDWRYWSQRGVTLARGGAPTDAEALEEGVILLPQGARGPAFLALPNHYVIRRYN
                     NSVSYALAIGLTADGIAGKPGLVGTWPDDAPLSRDQRIGAQRALTALGYDTGGVDGVV
                     GSNTRAALRRWQIASGRVADGYLTADLADELIRRAG"
     gene            1130..>1643
                     /locus_tag="VF686_07735"
     CDS             1130..>1643
                     /locus_tag="VF686_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661743.1"
                     /translation="MAKRPAVVKKSLSEANLAALGADRLAALLMEVAGVDLKRRLRME
                     LAAEVGAPDLALELDKRLNTLAASKARVSWRKRPELIADLQTLRRMIVDRLAPMDVRL
                     ALDRLVAWFDLYPALTARVKDPKGELTLVFDAAAADLAAVASLAGPDIAGPVLVEALS
                     TRLSEWASWVG"
BASE COUNT          228 a          651 c          542 g          222 t
ORIGIN      
        1 ggccggcttc gagggctgga aacagggctt cctgtcccgc cacggcggcg tccgtcgcga
       61 tcaatacgcc cgcgaactgg ccggcctgac gcccgatccc tccgtcatcc gcctcgacgg
      121 caaccagccc gagttcagcc gccccgccgg cgcctatatc cagtccgccg cctccgccac
      181 ccgcatagcc cagggccgcc agcgcaccga gcgcgtgccg tgggaggtca cccagcgctt
      241 cggcgtgccg gccgagatcc tcgtctccat ctggggcaac gaaagcagct tcggcgcggt
      301 ccagggcgac tatgacgtca tccgctccct ggccaccctc gccttcgacg gccgccgccg
      361 cgactgggcc gaggcccagc tgaaggacgc gctcgacatc gtcgtcgacg gccgccgcga
      421 ccgcgccggc ctgaaaggca gctgggccgg cgccatgggc cagacccagt tcatgcccga
      481 caacttcctg cgcctcgggg tcgaccagac cggcgacggc aaggtcgaca tctggggctc
      541 cgacgccgac tccctcgcct cggccgccaa tcttctggcc caggccggct ggaagcgcgg
      601 ccagagctgg ggctatgaga tcgtcctccc ctccggcttc gactacgccg aggccgaggg
      661 cccgcgccac gactggcgct actggtcgca gcgcggcgtc accctcgccc gcggcggcgc
      721 ccccaccgac gcagaggcgc tggaagaagg cgtcatcctg ctgccccagg gcgcgcgcgg
      781 tccggccttc ctggctctgc ccaaccacta tgtgatccgc cgctacaaca actcggtctc
      841 ctacgccctg gccatcggcc tgacggcgga cggcatcgcg ggcaagcccg gtctggtcgg
      901 cacctggccc gatgacgccc ccctgtcgcg cgaccagcgc atcggcgccc agcgcgccct
      961 gaccgcgctc ggctacgaca ccggcggcgt cgacggcgtc gtcggctcca acacccgcgc
     1021 cgccctgcgc cgctggcaga tcgcctccgg gcgcgtggcc gacggctatc tgaccgccga
     1081 tctggcggac gaactgatcc gaagggcggg ctgagacagg gccgcgcgca tggccaaacg
     1141 cccagccgtc gtaaagaaga gcctgtccga agccaacctc gccgccctcg gcgcggaccg
     1201 gctggcggcc ctgctgatgg aggtcgccgg cgtcgacctg aagcggcggc tgcgcatgga
     1261 gctggccgcc gaggtcggag cccccgacct tgcccttgaa ctcgacaaac ggctgaacac
     1321 cctcgccgcc agcaaggccc gggtctcgtg gcgcaagcgg cccgagctta tcgccgatct
     1381 ccagaccctg cgccgcatga tcgtcgaccg ccttgcgccg atggatgtcc ggctggcgct
     1441 ggaccggctg gtggcgtggt tcgacctcta tcccgccctg acggcccggg tgaaggaccc
     1501 caagggcgaa ctgaccctcg tcttcgacgc cgccgccgcc gacctcgccg ccgtcgcgtc
     1561 gctggccggg ccggacatcg ccgggccggt gctggtcgag gccctgtcca cccggctgtc
     1621 cgaatgggcg tcctgggtcg ggc
//
LOCUS       DASVVN010000480         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_18127, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000480 DASVVN010000000
VERSION     DASVVN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18127"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..601
                     /locus_tag="VF686_07740"
     CDS             239..601
                     /locus_tag="VF686_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8661744.1"
                     /translation="MITHVKFVSVPTRDQARALQFWTEKVGFRVLTDQPMGAQRWIEL
                     EVKGAQTRLVLFTPDGHGDRIGGFFNGSFACDDVEATHRQLSERGVEFTGPPTAQPWG
                     TYATFRDPDGNQFVISSR"
     gene            complement(617..1360)
                     /locus_tag="VF686_07745"
     CDS             complement(617..1360)
                     /locus_tag="VF686_07745"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX8661745.1"
                     /translation="MLRDLAHEAVTAVSSVDGKLLRTLRTLILRPGELTAAYAAGRRT
                     PYIGPLPLFLAANALFFAVQSASHVNILASPLASHLGDQDWQGLARALTERRLEANGM
                     ALAAYAPLFDQAAVLNAKALIVLMPAVFFLAPALLFARRHRPLALHGVFALHLYAFLL
                     LLFCACLAVSVVDVMAGGRGLAEPWIDVSLSLANLAACATYPYRATAVAYDARGWPRV
                     VKAAAMTVIAAALVIGYRFSIFLITLWTT"
     gene            complement(1544..>1732)
                     /locus_tag="VF686_07750"
     CDS             complement(1544..>1732)
                     /locus_tag="VF686_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661746.1"
                     /translation="GGFGSRSNGERPSAPRNAERPSGGFGSRNAERPSGGYGARPAAA
                     APAQRDSYGYRGPKRERV"
BASE COUNT          283 a          590 c          647 g          212 t
ORIGIN      
        1 ccggccagcg cctgtaggct cagcccgacg ctgtcgcggc cctcgccatg ggtgcgccgc
       61 cagagccaca ggtagacgac gaaggcggat ggcgcccggt cgtggccgac caggtcgcgc
      121 atcagcacgt cgacgacata ggggtcgagc ggcagcgtca ttcccggcac tatagtcctt
      181 atcggaccca ttgctccggc cgtcgccccg ggcgagaggc gggcggggag gggaccgcat
      241 gatcacccac gtgaagtttg tcagcgtgcc gacgcgcgac caggcgcggg cgctgcagtt
      301 ctggaccgaa aaggtgggct tccgcgtgct caccgaccag ccgatgggcg cgcagcgctg
      361 gatcgagctg gaggtgaagg gggcgcagac gcggctggtg ctgttcacgc cggacggcca
      421 cggggaccgc atcggcggtt tcttcaacgg ctcgttcgcc tgcgacgacg tcgaggccac
      481 gcaccggcag ctgagcgagc gcggcgtcga gttcaccggg ccgccgacgg cgcagccgtg
      541 ggggacctac gccacgttcc gcgacccgga cggcaaccag ttcgtcatca gcagccggtg
      601 aaccgggcgc caggccctag gtcgtccaca aggtgatcag aaagatgctg aagcggtagc
      661 cgatgacgag ggcggccgcg atcacggtca tggcggccgc cttgacgacg cgcggccacc
      721 ctctggcgtc gtaggcaacc gccgtcgccc gataagggta ggttgcgcag gccgcgaggt
      781 tcgccagcga aaggctgacg tcgatccacg gttccgcgag gccgcgtccg cccgccatga
      841 cgtcaacaac cgacaccgcc aggcaggcgc agaacaggag aagcaggaag gcgtacaggt
      901 ggagcgcgaa cacgccgtgg agcgcaagcg gccgatgcct gcgggcaaac agcagcgccg
      961 gggccagaaa gaagacggca ggcatcagca cgatcagcgc cttggcgttc agcaccgccg
     1021 cctggtcgaa cagcggcgca tatgccgcca gcgccatgcc attcgcctcg aggcggcgct
     1081 cggtcagcgc ccgggcaagc ccctgccagt cctgatcgcc caggtgtgag gcgagcgggc
     1141 tggccagtat gttgacatgc gacgccgact gcacggcgaa gaacagcgcg ttcgcggcca
     1201 ggaacagcgg cagcggaccg atgtagggcg tgcggcgccc ggccgcgtac gcggcggtta
     1261 gctcgccggg tcgcaggatc agcgtgcgga gggtccgcag cagcttcccg tccacgctgc
     1321 tgacggccgt gaccgcctca tgggcgagat cgcgcagcat gaggtgacgg gatcgcaccg
     1381 gccgctcgcc gcaggcggga cagaaggcgg agtccaggcc ggtgctgcag ttcgggcaga
     1441 cccagggcgc ccggctggcc atgacactcc ctagcacggc ggagctgcgc tccgctcctg
     1501 ccggggagcg gtcagcgcgc cggggcgcgc ggaccgtggg gcatcagacc cgttcccgct
     1561 tggggccgcg gtagccgtag ctgtcgcgtt gcgccggagc cgccgccgcg ggacgcgcgc
     1621 cgtagccgcc cgaaggccgc tcggcgttgc gcgagccgaa gccgcccgac ggacgctcgg
     1681 cgttgcgcgg ggccgacggc cgttcgccgt tgctgcgcga gccgaagccg cc
//
LOCUS       DASVVN010000481         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_71032, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000481 DASVVN010000000
VERSION     DASVVN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71032"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VF686_07755"
     CDS             <1..580
                     /locus_tag="VF686_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661747.1"
                     /translation="RFAVSLTPGTRGGDMFRQGSLDTLCGIYAIINSTHEVCATWDSR
                     LKPDHHRALFRVLCEELATEGDLAGALAGEGMGVRTLRKLLRRAHMYVEDETGLPFTH
                     KRAFNAEPVALDVYWEAIRDHVEENGPGSVLISLTGRHCHWSCVRSVSESGMVLRDSD
                     GLIRLSRARCTTGAGDDRRHHVLWPTETLLIT"
     gene            714..839
                     /locus_tag="VF686_07760"
     CDS             714..839
                     /locus_tag="VF686_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661748.1"
                     /translation="MLRALRTGLFIGVFLGMSACAAMPDSGARGPYGSVEVGRTG"
     gene            935..>1615
                     /locus_tag="VF686_07765"
     CDS             935..>1615
                     /locus_tag="VF686_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX8661749.1"
                     /translation="MLALAIVVVLLLVVLNGLFAMTELAVVSSRRSKLQSRAERGDKG
                     ARAALHLAEDPTHFLSAVQVGITLIGILAGAYGQAAIGGELNRIIETSVPALAQWSHV
                     IATGIVVVFITYVSVIVGELVPKRLALIFPESIASKMAAPISTLSIVLKPFVVLLTVS
                     TSGILKIMGVKDRDGSDVTQEEVETMIAEGTASGLIEPEEQEMIEEILRLGDRPIRVA
                     MTPRHEVFW"
BASE COUNT          248 a          550 c          540 g          277 t
ORIGIN      
        1 ccgatttgct gtatcgctga cgccggggac acgagggggc gacatgttca gacagggcag
       61 cctggatacg ctgtgcggca tctacgccat catcaacagc acccatgagg tctgtgcgac
      121 ctgggacagc cggctgaagc ccgaccatca ccgggcgctg ttccgcgtcc tgtgcgagga
      181 actggcgacc gaaggcgatc tggcgggcgc gctcgccggc gaggggatgg gggtgcggac
      241 cctgcgcaag ctgctccgcc gggcgcatat gtatgtcgag gacgagaccg ggctgccgtt
      301 tactcacaag cgcgccttca acgccgagcc cgtagcactg gatgtctact gggaggcgat
      361 ccgcgaccat gtcgaggaga atggccccgg ctcggtcctg atcagcctca ccggccgcca
      421 ttgccactgg tcctgcgtcc gcagcgtcag cgagagcggg atggtgctgc gcgactccga
      481 cggcctgatc cggctgagcc gggcgcgctg caccaccgga gccggcgacg accggcggca
      541 ccatgttctg tggccgaccg agaccctgct gatcacctga aacccgcgcg ggccggattc
      601 gccctatagg gcgtgcaggc cggttaacgc cgcgcctgtc cggggcaagc gcttgcgtgc
      661 aaccggaatc gggcccctac gttcgcatcg tggacgttag ggattcagta accatgcttc
      721 gcgcgcttcg gaccggcctg ttcatcggcg tcttcctcgg catgtcggcc tgcgccgcca
      781 tgcccgacag cggcgcgcgc gggccctatg gctcggtcga ggtcggccgc acggggtgac
      841 gctgcaagga aaccgtggcg gcgagggcgg attcccccta tcatcagtcc cgcggacgcc
      901 gactgtcgcg ccgcgccggt tccgccccca ctagatgtta gccctcgcca tcgtcgtcgt
      961 tctgctcctc gtggtgctca acggcctgtt cgccatgacc gagctggcgg tcgtctcctc
     1021 acgcagatcc aagcttcaga gccgcgccga gcggggcgac aagggcgcgc gcgcggccct
     1081 gcatctcgcc gaggatccga cccatttcct gtccgcggtc caggtcggca tcaccctgat
     1141 cgggattctg gcgggcgcct acggccaggc ggccatcggc ggcgaactga accgcatcat
     1201 cgagacctcg gtcccggccc tcgcgcagtg gtcgcatgtg atcgccaccg gcatcgtggt
     1261 cgtcttcatc acctatgtgt cggtgatcgt gggcgagctg gtgcccaagc ggctggcgct
     1321 gatcttcccg gaatcgatcg cctccaagat ggccgcgccg atctcgaccc tctcgatcgt
     1381 gctcaagccc ttcgtggtcc tgctgaccgt ctcgacgtcc ggcatcctca agatcatggg
     1441 cgtcaaggac cgcgacggtt cggacgtcac ccaggaagag gtcgagacca tgatcgccga
     1501 gggcacggcg tcgggtctga tcgagcccga ggagcaggag atgatcgagg agatcctgcg
     1561 tctcggcgac cgtcccatcc gcgtggccat gacgccgcgc cacgaggtgt tctgg
//
LOCUS       DASVVN010000482         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_518321,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000482 DASVVN010000000
VERSION     DASVVN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518321"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF686_07770"
     CDS             complement(<1..378)
                     /locus_tag="VF686_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX8661750.1"
                     /translation="MTGRIHRFDALDNFRDYGDYAAGTGRHIAPGRLFRSAHQARVSA
                     ADLERLDALGMAAIVDLRRPSERRDQPSKRPAGFTGVVIESDHDDGAEAPHLTFLRTA
                     DLTPESGRRFMTDTYRRLPFEASH"
     gene            complement(407..712)
                     /locus_tag="VF686_07775"
     CDS             complement(407..712)
                     /locus_tag="VF686_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8661751.1"
                     /translation="MADEFDISRLDEVIHGRVRLGIMAVLSGVDSADFNTLKARLQTT
                     DGNLSVHLRKLEDAGFVAVSKRFEGRKPLTEASMTPAGRKAFVAYLDAMQGLVGKSG"
     gene            complement(715..1338)
                     /locus_tag="VF686_07780"
     CDS             complement(715..1338)
                     /locus_tag="VF686_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661752.1"
                     /translation="MIDKTEDPAADIAWMRRLAEEGAEAPMQGASILMSAGLIFGAAS
                     IFHWAQITGHVPQLDGITGIGWLVATLLFMLVLAVIISRLNRTGGIMTASNRATNTVW
                     SALGWGIFALFGSMAVLGWRVGGEGVEIAFGLIPSVIMVFYGIGWAVSAAMYKSRTLW
                     GLAAGSFVAAPLLAALTGRADQYLAYAAALLLLMALPGYLLMRGARR"
     gene            complement(1352..1921)
                     /locus_tag="VF686_07785"
     CDS             complement(1352..1921)
                     /locus_tag="VF686_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661753.1"
                     /translation="MSAVAFDLAADRPAPTVPVCAGRVLVLAGVAFGAANLIQWGVMS
                     GALGWHPAVLALSWPVAVGAFLIGVFHLRRTGGEAGRQVGGWSRIAILIHIGAALALA
                     GLSAATGDWELMRGTSAVGLTLYGLAWAVAAARTGAVNMAALAAVAFAGVAGMALRFG
                     TADQYLIYVCALALVALIPGLWLALGRRL"
BASE COUNT          418 a          765 c          817 g          336 t
ORIGIN      
        1 gtgggatgcc tcgaacggca gccgccggta ggtgtcggtc atgaagcggc ggccggactc
       61 gggcgtcagg tcggcggtcc tcagaaaggt gaggtgcggg gcctccgcgc cgtcgtcgtg
      121 gtcgctctcg atcactacgc ccgtaaagcc ggcggggcgt ttcgacggct ggtcgcgccg
      181 ttcggatggg cggcgcaaat cgacgatggc ggccatgccc agggcgtcca gccgctccag
      241 gtcggcggcg ctgacccgcg cctgatgcgc cgagcggaac aggcggccgg gcgcgatatg
      301 gcggcccgtc ccggcggcgt agtcgccgta gtcccggaaa ttgtcgaggg cgtcgaagcg
      361 gtggatgcgg ccggtcatgg cggccttcta gccgaggcgc gggcctctaa ccactcttgc
      421 cgaccagacc ctgcatggcg tcgagatagg cgacgaaggc cttacgcccg gcgggggtca
      481 tggacgcctc ggtcaggggc ttgcggccct cgaaccgctt gctcacggcg acgaagccgg
      541 cgtcctcaag ctttcgcaga tggaccgaca gattgccgtc ggtggtctgc agccgggcct
      601 tgagcgtgtt gaaatcggcg ctgtcgacgc cggacaggac ggccatgatt cccagccgga
      661 cccggccgtg gatgacctcg tcgagacggc tgatgtcgaa ctcgtcagcc acgctcagcg
      721 cctcgcccca cgcatcagca ggtagccggg cagggccatg agcaggagca gggcggcggc
      781 gtaggccaga tactggtctg cgcgaccggt cagagccgcc agcagcggcg ccgcgacgaa
      841 cgagccggcg gccaggcccc acagggtgcg gctcttgtac atcgccgcgc tgaccgccca
      901 gccgatgccg taaaagacca tgatgacgga cgggataagc ccgaaggcga tttcgacgcc
      961 ttcgccgccg acgcgccagc cgagcacggc catggatccg aacagggcga aaatgcccca
     1021 gcccagtgca ctccagaccg tgttggtggc ccggttgctt gcggtcatga ttccgccggt
     1081 gcggttcaac cggctgatga tgacggcgag gacaagcatg aacagaaggg tggcgacgag
     1141 ccagccaatg ccggtgattc cgtccagttg cggaacgtgg cccgtgatct gggcccagtg
     1201 gaagatgctg gcggcgccga aaatcaggcc cgcgctcatc aggatggatg cgccctgcat
     1261 gggcgcctcg gcgccttcct cggccagccg gcgcatccag gcgatgtcag cggcggggtc
     1321 ttcggtcttg tcgatcatcg gtctttcgtc cttacaggcg acggccaagg gccagccaca
     1381 gcccgggaat cagggccacg agggcaagcg cgcaaacata gatcagatat tggtccgcgg
     1441 tcccgaaccg cagggccatt ccggcgacgc cggcgaaggc gacggcggcc aaggccgcca
     1501 tgttgacggc gccggtgcgc gcggcggcca cggcccaggc gaggccgtac agggtgaggc
     1561 cgaccgccga ggtcccgcgc atcagctccc agtcgcccgt ggcggccgag aggccagcca
     1621 gggccagggc cgcgccgatg tggatcagga tggcgatgcg tgaccagccg ccgacctggc
     1681 ggccagcctc gcccccggtg cgccgcaggt ggaagacgcc gatcagaaag gcccccacgg
     1741 cgaccggcca gctgagggcg agcacggccg gatgccagcc caaggcgccg ctcatgacgc
     1801 cccactggat caggttggcc gcgccgaacg cgacgccggc cagaaccaga acacggccgg
     1861 cgcagaccgg gacggtgggc gcggggcggt cggcggcgag gtcgaaggcg acggcggaca
     1921 tgcgagaaat ctccctgttg cctctttctc ccacatcaac tttgaaaagt aaagcgcttc
     1981 atacatggtt cgctgcggac gttgcgaacg cgaaaaaaca cagtctactg cgtctactca
     2041 gtctactccg tccaatgaac ggctggtccc ggagatcagg aaaacaccgt ccgaagagtc
     2101 cgaagagtcc acttctccgc cggccgacgg aggcggccgg atctgtccac cgggtcggaa
     2161 gccacgattc ttccaccttg catcgatgtc aaagaccggg cccgaaggcc agatcatgag
     2221 aacatgcgca tgcgtcagtt ttggacgtag gcgtggagcc ccgatgacca tcaccttctc
     2281 ccgcatcgcc tatcaggcgc tcgaggtctg caacggcgtg acgatggccg cgatcg
//
LOCUS       DASVVN010000483         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_518337,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000483 DASVVN010000000
VERSION     DASVVN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518337"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VF686_07790"
     CDS             <1..108
                     /locus_tag="VF686_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661754.1"
                     /translation="VGRVYHLDRGFERLEEKLSACGAEIRRIKSDGHEH"
     gene            98..586
                     /locus_tag="VF686_07795"
     CDS             98..586
                     /locus_tag="VF686_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX8661755.1"
                     /translation="MSTEVQEHEADAAATPPEPLRLLAEDADDLHIISAALQDAIMRP
                     VDIVWEPGARRVTIKLSRFCWECGGTRVMAAMQFGDVVAVKSRRLPRLPESALELLAM
                     DFVPDEAPGGRVILMFAGGGDLRIDVECLDAVLTDLSERWPAKVAPTHLSPETSDRAD
                     PA"
     gene            583..1062
                     /locus_tag="VF686_07800"
     CDS             583..1062
                     /locus_tag="VF686_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX8661756.1"
                     /translation="MSEPNPEKHRLASIDLDAATLSSATAEIEHERRVAIFDLIEKNS
                     FEPAGAGGGPYALKLSMVDGRLAFDITGPDFHRVHGLSLTPLNKVVKDYIAICDSYYE
                     ALRGTSPSQIEAVDMGRRGLHNEGAELLKARLDGKIAIDHETARRLFTLVTALYRRG"
     gene            1166..1387
                     /gene="infA"
                     /locus_tag="VF686_07805"
     CDS             1166..1387
                     /gene="infA"
                     /locus_tag="VF686_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8661757.1"
                     /translation="MAKEELLEFPGVVSELLPNATFRVLLEGNDHEIIAHTAGKMRKN
                     RIRVLAGDKVLVEMTPYDLTKGRITYRFK"
     gene            1400..1975
                     /locus_tag="VF686_07810"
     CDS             1400..1975
                     /locus_tag="VF686_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX8661758.1"
                     /translation="MSRPELVLASASPRRIELLALVGITPDRVDPADIDETPLRDETP
                     TRLAARLAVSKSQAVAARSPDAVVLAADTVVAVGRRLLEKAADEAEAARFLKLLSGRN
                     HRVFTGVAVTAAGRTTQRVVDTRVSFKVLSDAEIAAYVASGEWRGKAGGYGIQGRAGA
                     FVTRLVGSYPAVMGLPLFETVNLLNGAGWRA"
     gene            1972..>2268
                     /locus_tag="VF686_07815"
     CDS             1972..>2268
                     /locus_tag="VF686_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8661759.1"
                     /translation="MSVEVFLDDTPGELRAAIRRDGHFEHLLIESEREEPQTRLGARS
                     VARVRQAAPGLKGAFVDLGAGGEAFMSLRGGVDAPVGAKVEVELAAQQRAGKAPV"
BASE COUNT          357 a          763 c          809 g          339 t
ORIGIN      
        1 gtgggccgcg tctaccacct ggaccgcggc ttcgagcggc tggaagaaaa gctgagcgcc
       61 tgcggagccg aaattcgccg catcaagagc gatgggcatg agcactgagg tccaggagca
      121 tgaggccgac gcagcggcga cgccgcccga gcccctgcgc ctgctcgccg aggacgccga
      181 tgatctgcac atcatctccg cggcgctgca ggacgcgatc atgcgtccgg tcgacatcgt
      241 ctgggagccc ggcgcccggc gggtgacgat caagctgagc cggttctgct gggaatgcgg
      301 cggcacacgg gtgatggcgg cgatgcagtt cggcgacgtg gtcgcggtca agagccgccg
      361 cctgccgcgc ctgccggaaa gcgcgctgga gctgctggcc atggatttcg tgcccgacga
      421 ggcgccgggc ggccgggtca tcctgatgtt cgccggcggc ggcgacctgc gcatcgacgt
      481 cgaatgtctg gacgcggtgc tgacggacct gtccgaacgc tggccggcca aggtcgcgcc
      541 gacgcacctt tcgccagaga cgtcagaccg ggcggatccc gcgtgagcga acccaacccc
      601 gaaaaacatc gcctcgcctc gatcgacctg gacgccgcga ccctgtcgtc cgcgacggcc
      661 gagatcgagc acgagcggcg ggtggccatt ttcgacctga tcgagaagaa cagcttcgag
      721 ccggcgggcg ccgggggcgg cccctatgcg ctgaaactgt cgatggtgga cggccggctg
      781 gcgttcgaca tcacgggccc ggacttccac cgggtccacg gcctgtcgct gacgccgctg
      841 aacaaggtgg tgaaggacta tatcgccatc tgcgacagct attacgaagc cctgcgcggg
      901 acctcgccgt cgcagatcga ggcggtcgac atgggccggc gcggcctgca caacgaaggc
      961 gccgagctgc tgaaggcgcg gctggatggc aagatcgcga tcgaccacga gacggcgcgg
     1021 cggctgttca cactggtgac ggctctgtac cggcgcggtt gatctcgact atcccggcgt
     1081 gttcgggcat taggcccgct cttttcgacg cgcgctttcg cccggcgcgc gttctgacct
     1141 cgattcacag ggcgcgagag gccgcatggc taaggaagaa ctgctcgagt ttcccggcgt
     1201 cgtcagcgaa ttgctgccga acgcgacgtt ccgcgtgctg ctcgagggga acgaccacga
     1261 gatcatcgcc cacaccgccg gcaagatgcg caagaaccgc atccgcgtgc tggccggcga
     1321 caaggttctg gtcgagatga ccccctatga cctgaccaag ggtcgcatca cctaccgctt
     1381 caagtaagga cgggctcaca tgagccgtcc cgaactcgtg ctggcctcgg ccagcccgcg
     1441 ccgcatcgaa ctgctggcgc tggtcgggat cacgcccgac cgcgtcgatc ccgccgacat
     1501 cgacgagacc ccgctgaggg acgagacgcc gacccgcctc gcggcgcggc tggccgtctc
     1561 caagtcgcag gccgtcgcgg cccgatcgcc ggacgccgtg gtgctggccg ccgacaccgt
     1621 cgtggccgtt ggccggcgtc tgctggagaa agccgccgac gaggccgagg cggcgcgctt
     1681 cctgaaactg ctgtccggcc gcaatcaccg cgtcttcacc ggcgtggccg tgaccgccgc
     1741 cggccgcacg acgcagcggg tggtcgacac ccgtgtctcg ttcaaggttc tttccgacgc
     1801 cgagatcgcc gcctacgtcg cctcgggcga atggcggggc aaggcgggcg gatacggcat
     1861 ccagggccgg gcaggggcct tcgtcacacg tctggtcggc agctatcccg ctgtcatggg
     1921 cctgccgctg ttcgagacgg tcaatctgct gaacggcgca ggctggcggg cgtgagcgtc
     1981 gaggtcttcc tcgacgacac gccgggcgag ctgagggccg ccatccggcg cgacggccat
     2041 ttcgaacacc tgctgatcga gagcgagcgc gaagagccgc agacccggct gggcgcgcgg
     2101 tccgttgcgc gcgtgcgtca ggcggcgccg ggtctgaagg gcgccttcgt cgatctgggc
     2161 gcgggcggcg aggccttcat gtccctgcgc ggcggcgtgg atgcgccggt gggggccaag
     2221 gtcgaggtcg aattggccgc ccagcagcgc gcggggaagg ctccggtc
//
LOCUS       DASVVN010000484         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_177797,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000484 DASVVN010000000
VERSION     DASVVN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177797"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1240
                     /locus_tag="VF686_07820"
     CDS             35..1240
                     /locus_tag="VF686_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8661760.1"
                     /translation="MEFRHSDRALHWQERVGRFLEDHVLPREAEYRAQVREDHTRQPP
                     AMEELKARARQAGLWNMFLPGEHGAGLTNLEYAPLAELMGRHLWSAEVFNCNAPDTGN
                     MEVLHMYGNEAQQAKWLKPLMAGEIRSAFLMTEPEVASSDATNIQTRIVRDGDHYVIN
                     GRKWWSTNMAHPNVSVTIVMGKTDPDAATHAQQSQIIVPVDTPGFRVERMLSVFGYDE
                     LPIGHAEVVLENVRVPVENLIAGEGRGFEIAQGRLGPGRIHHCMRVIGIAERALELMV
                     QRLLSRTAFRKSLAEHSVWEQRVGEARTNIEMCRLLVLKAAWLMDEAGAKNAKSEIAQ
                     IKVAAPRMALQVIDDAIQAFGGAGVSSDTPLAEMYAMVRTLRIADGPDEVHNRTVARL
                     EYAKHGGRG"
BASE COUNT          288 a          551 c          525 g          246 t
ORIGIN      
        1 ctccccatga aggggaggga gaaggagatt tcccatggaa ttccgtcaca gcgacagggc
       61 cctgcactgg caggagcggg tggggcggtt ccttgaggac cacgtgctgc cgcgcgaggc
      121 ggaataccgg gcccaggtcc gcgaggatca cacccgccag ccgcccgcca tggaagagct
      181 gaaggcccgg gcgcgccagg ccggtctgtg gaacatgttt ctgcctggtg aacacggggc
      241 cggtctgacc aacctcgaat acgccccgct ggccgaactg atgggccgcc acctgtggtc
      301 ggcggaggtg ttcaactgca acgcccccga caccggcaat atggaagtgc tccatatgta
      361 cggaaacgag gcccagcagg cgaaatggct caagccgcta atggccggcg agattcgctc
      421 ggccttcctc atgaccgagc ccgaggtggc ctcttcggac gcgaccaaca tccagacccg
      481 gatcgtgcgc gacggcgacc actacgtcat caacggccgc aaatggtggt cgaccaacat
      541 ggcgcacccc aacgtctccg tgaccatcgt catgggcaag accgaccccg acgccgccac
      601 ccacgcccag cagtcccaga tcatcgtccc cgtcgacacg ccgggcttcc gtgtcgaacg
      661 gatgctgtcg gtgttcggct atgatgagct tccgatcggc cacgccgaag tggtgctgga
      721 gaacgtccgc gttccggtgg agaacctgat cgccggcgag gggcgcggct tcgagatcgc
      781 ccagggccgc ttggggccgg gccgcatcca ccactgcatg cgcgtcatcg gaatcgccga
      841 acgcgcgctg gagctgatgg tccagcgcct gctgtcgcgc accgccttcc gcaagtcgct
      901 ggcggagcat tcggtctggg aacagcgcgt cggcgaggcc cgcaccaata tcgagatgtg
      961 ccgcctgctg gtcctcaagg ccgcttggct gatggacgag gccggcgcga aaaacgccaa
     1021 gtccgagatc gcccagatca aggtcgccgc cccgcgcatg gcgcttcagg tcatcgacga
     1081 cgccatccag gccttcggcg gcgccggcgt ctcgtccgac acgcccctgg ccgagatgta
     1141 cgccatggtc cgcaccctgc gcatcgctga cggccccgac gaggtccaca accggacggt
     1201 ggcaaggttg gaatacgcga agcacggggg aaggggctga tagtccgaag gcctcgtctc
     1261 ctccccgtct ccgatgggga ggtggcgcgg cgcgtcttcg cgccgtgacg gaggggggcc
     1321 gcgccgcacc ccttcacccc tccaccccct tcggggtccc cctccccatc gccttcggcg
     1381 acagggagga gacactgctg gcaatccgcc gccagccaga cgactttgcg cggttaaccc
     1441 gttgatcctg cacaggatca aacgatgacc agcgtcattt cgtagccata ggtaccgacc
     1501 gtgcgacact cctccccgtc ccgtcgcagg ggaggcgcaa aggccttgcg ttctttggcg
     1561 gcgggatgtg gagaagcggg gcccggttcc cggctgtcaa agcaaggcag
//
LOCUS       DASVVN010000485         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_177854,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000485 DASVVN010000000
VERSION     DASVVN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177854"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF686_07825"
     CDS             complement(<1..326)
                     /locus_tag="VF686_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8661761.1"
                     /translation="MRSLAFNIAYWLLSIVYTLAAAFAALTPGREATQWVIRRYVKRM
                     VQAMWLLAGIRIQVRGKERLPTGAFIIASKHQSWGDGFATYDQFDDLAFVTGDHLEKF
                     PLLGTVL"
     gene            complement(364..1419)
                     /locus_tag="VF686_07830"
     CDS             complement(364..1419)
                     /locus_tag="VF686_07830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8661762.1"
                     /translation="MTVKVETTLRGPQAYGLARKVVDRMEAAGVWPTPLNFELWTHAL
                     GEPDGALAGEIQRITDAGEPFTEAVTDALAAQHLPRGRLTDQIRDAGQVLDRELSSVS
                     SVIAEAQKSQAAYGQTLEQAATSIEAAADGEGLQTIVSDLTTATRQVQNETDSLEKKL
                     ETSTQEIVRLREHLELVRRDAMTDALTELANRKAFDEHLAAACDHAEAQGGALTLAVL
                     DIDHFKRFNDTWGHQTGDQVLRYVASVLGRTAANPRFAARFGGEEFAIVFPYESAAMV
                     ETALEGVLKEIASRSLRRRSTNDDLGAVTISAGFAVRQRGETASAFLERADAALYASK
                     RGGRNRVSCAERLPQAA"
     gene            complement(1511..>2137)
                     /locus_tag="VF686_07835"
     CDS             complement(1511..>2137)
                     /locus_tag="VF686_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEX8661763.1"
                     /translation="TGRAGKLGQTFMIVTPADDRSLDKVLKLIKMDPIELTLDGVDFA
                     PGKAPSSRDGGSSDKPARGRSRGSRSEPKADAAPAQQDETAAPLEAPAPAEAPVAAAE
                     PAAEGAPTARRSRSRRSARPAAPTAEAPAAEARAAAPERKPADRGRPAPTPAPAKETS
                     ERPVQETHLRQSDRRGDRADAQPERSNGRPFGEGDVPAFLRRPVSIKG"
BASE COUNT          287 a          676 c          820 g          354 t
ORIGIN      
        1 aggacagtgc cgagcaaggg gaatttctcc agatggtcgc cggtgacgaa ggccagatcg
       61 tcgaactggt cgtaggtggc gaagccgtcg ccccagctct ggtgtttgga ggcgatgatg
      121 aaggcgccgg tcggcagccg ctccttgccg cgcacctgga tgcggatgcc ggccagcagc
      181 cacatggcct ggaccatgcg cttcacatac cggcggatga cccattgcgt cgcttcccgc
      241 ccgggggtca gggccgcgaa ggccgccgcc agggtgtaga cgatcgacag gagccagtag
      301 gcgatgttga aggcgaggct gcgcatccgg cgactatggc gtgaattggc gagcggggaa
      361 gggtcaggcc gcctggggca gccgttcggc gcaggatacg cggttgcggc cgccgcgctt
      421 ggacgcatag agggccgcgt cggcccgttc gaggaaggcc gaggcggttt cgccccgctg
      481 acggacggcg aagcccgccg atatggtcac ggcgcccaga tcgtcgttgg tcgagcggcg
      541 gcggagcgac cgggaggcga tctccttgag cacgccctca agggccgtct cgaccatggc
      601 ggcgctctca taggggaaga cgatggcgaa ctcctcgccg ccgaagcggg cggcgaagcg
      661 cggattggcg gcggtgcggc ccagcacgct ggcgacatag cgcaggacct gatcgccggt
      721 ctggtgcccc caagtgtcgt tgaaccgctt gaaatggtcg atgtcgagca ccgccagggt
      781 cagggccccg ccctgtgcct cggcgtggtc gcaggcggcg gccagatgct cgtcgaaggc
      841 tttgcggttg gccagctcgg tcagggcgtc cgtcatggcg tcgcggcgca ccagttccag
      901 atgttcgcgc aggcgcacga tttcctgggt ggaggtttcc agcttctttt cgagcgagtc
      961 cgtctcgttc tggacctgac gtgtggccgt ggtcagatcg gagacgatgg tttgcaggcc
     1021 ttcgccgtcc gccgccgcct ctatgctggt ggcggcctgt tcgagggtct ggccgtaggc
     1081 ggcctgcgac ttctgcgcct cggcgatgac gctggagacg ctggacagtt cgcggtccag
     1141 aacctggccg gcgtcgcgga tctgatcggt caggcgaccg cgggggaggt gctgggccgc
     1201 caaggcgtcg gtaacggcct cggtgaaggg ctcgcccgca tcggtgatgc gctggatctc
     1261 gccggcgagg gcgccgtctg gctctccgag ggcatgggtc cagagttcga aattcagcgg
     1321 cgtgggccag acgccggcgg cctccatccg gtcgacgacc ttgcgtgcga gcccataggc
     1381 ttgcggacct cgcagggtgg tttcgacctt gaccgtcatt cacacgtccc caggctcaag
     1441 ccggagcggc gatcctgtcc gatcaggcta gaccgccggt tcgtaacgcc gggttaaacc
     1501 gggagccgga tcagcccttg atcgaaaccg ggcggcgcag gaaggcgggc acgtcgccct
     1561 cgccgaacgg acgcccgttc gagcgttccg gctgggcgtc ggcgcgatcg ccgcggcggt
     1621 ccgactggcg caggtgggtt tcctgcaccg gacgttccga cgtctctttc gcgggagccg
     1681 gggtcggggc ggggcgcccg cggtcggcgg gcttgcgctc gggcgcggct gcccgggctt
     1741 cggcggcggg cgcctccgcg gtcggagctg cgggcctggc gctgcggcgg ctgcgcgagc
     1801 gacgggcggt cggcgcgcct tcggcggcgg gctcggcggc ggcgacaggc gcctcggccg
     1861 gagcgggcgc ctctaggggc gcagccgttt cgtcttgctg cgccggagcg gcgtcggcct
     1921 tgggctcgct gcgtgaaccg cggctgcggc cgcgtgcggg cttgtcggag gagccgccat
     1981 cgcgcgagga cggggccttg cccggggcga agtcgacgcc atccagggtt agctcaatgg
     2041 gatccatctt gatcagcttc aggaccttgt cgagcgaacg gtcgtctgct ggcgtcacga
     2101 tcatgaaggt ctggcccagc ttgcccgcac gacccgt
//
LOCUS       DASVVN010000486         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_123955,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000486 DASVVN010000000
VERSION     DASVVN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123955"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1070..1230
                     /gene="ssrS"
                     /locus_tag="VF686_07840"
     ncRNA           1070..1230
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF686_07840"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1390..>1741
                     /locus_tag="VF686_07845"
     CDS             1390..>1741
                     /locus_tag="VF686_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX8661764.1"
                     /translation="MTDKHQLRAAMRATRKRLAGLDAGAAGRAAEQAGALPPGRIVAV
                     YRAIGSELDVDALSLALSRAGRELCLPVVIERNAPMIFRRWSPGEPLELDEAGVPAPF
                     PLAEIVEPDLILTPL"
BASE COUNT          209 a          653 c          595 g          284 t
ORIGIN      
        1 gccagcgccg ccgcggccgg ggtcttcgtc ttcggcctgc gcgatctggc cgccgccggc
       61 cgatccgacc ggctggttca gcccgatccc gccgccatgg gccgcgccct gccgggacgc
      121 cgtcgcgtgc ttgtcaccgg cgccaccggt ttcatcggcc gccggctggt cgtcgccctg
      181 acgggcgccg ggcacgaggt catcgctctg gcccgcaatc cgaagaccgc cgatctgccc
      241 gcgccgatca cggtggtgac cgatctggtc cagatcgcag ccgacacccg catcgacgcc
      301 gtcgtgaacc tcgccggcga gcctatcagc gacgggctgt ggaccttggc caaacgccgc
      361 cgcatccttc gctcccgtct ggtggtcacc cgcgacgtcc gccgcctcat cgcccggctc
      421 gaccacaggc cggaggtcct cgtcagcggc tccgccatcg gctggtacgg cctgcatgcc
      481 gatgagacgc tggatgaagc gggtccggcc cacgcgtgtt ttagccaccg gctgtgcgat
      541 gcctgggaac gggcggcggc gcctgtcgag accatgggcg tccggctggt caccctgcgc
      601 atcggcctgg tgcttggaac cgcgggcggc atgctgtcgc ggctgctgac gcccttcgaa
      661 ttcggcctcg gcggacccat gggaacgggc cgccagtgga tgtcgtggat cgcgctggac
      721 gactgcgtac ggctgatcct ccacgccgtc gccgagccgc gggtggcggg tccgctcaac
      781 gccacagcgc cagtccccgt gtccaaccgc gatctgtcgc gcgcccttgg ccgggcgctg
      841 aaccggccgg cggtcatggc cgcacccgcc ctgccgctcc gtctggcgct cggagatttc
      901 gcggacgagc tcttgctggg cgggcagcgg gtcgtaccgg ccaaggcgct cgccaccgga
      961 ttccacttcg atcatccgga actggagggc gcgctggccg ccatcgtcgg cgcccggtcg
     1021 cgccccactg gcgcgaaggg ccgcaaacga ctatcttcct cgacggcggg tcttgctgcg
     1081 gccctcgtcg aaggcaacat ttccttgcga ccttaattca ctcgaaggga gctgtccctg
     1141 accaggctcg tgggcctggg cacacggcgc ccacctggct acccaggtcg agaggattta
     1201 aacgtccttc acggttctcg cggcgccgcc aacctctttc ctttgtgcgc taacgctcgc
     1261 cccacggggc ctcgctgcat gagcgcggct tcgtcaccct ccggcttgac cggagggtcg
     1321 ctgcttgagc gcggtcgggc cgcgcgctac ggctccttgg cgtgttctac gcggccggac
     1381 ggcgtctcca tgaccgacaa gcatcagctc cgcgccgcca tgcgggccac gcgcaagcgt
     1441 ctggcggggc tggacgcggg cgccgccggc cgcgcggctg aacaggccgg cgccctgccg
     1501 cccgggcgga tcgtcgccgt ctatcgcgct atcggctcgg aactggacgt cgacgccctg
     1561 tcgctggcgc tcagccgggc cgggcgcgag ctgtgtctgc cggtggtgat cgagcggaac
     1621 gcgccgatga tcttccgccg ctggtcgccg ggcgagccgc tggagctcga cgaggccggc
     1681 gtgcccgcgc cgttcccgct ggccgagatc gtcgagcccg acctgatcct gaccccgcta
     1741 c
//
LOCUS       DASVVN010000487         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_18390, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000487 DASVVN010000000
VERSION     DASVVN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18390"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VF686_07850"
     CDS             complement(<1..971)
                     /locus_tag="VF686_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX8661765.1"
                     /translation="MAGASPQGEDGAGAAASNSQTPSDKSVAPAHPHPAAAGEGGHVK
                     AGKWGLIVGAIGVVFGDIGTSPLYALREAIGHAQGGVGGDLAIVGVVSLAFWALMIVV
                     TFKYVMFLMRADNKGEGGTLALMALATHAVGRRSGLIFILGVCGAALFYGDGIITPAI
                     SVLSAIEGVKDAPGVGGKLDPFIVPVSAGILIALFLVQARGTAGLARFFGPITAIWFL
                     SLGVLGLIHIFDDVSILRALSPHYGVMLLINDGFLGFVILGSVFLAVTGAEALYADMG
                     HFGKAPIRAGWVAFVLPCLTLNYLGQGALILSHPGAAENPFWSMVPSA"
     gene            1146..1616
                     /locus_tag="VF686_07855"
     CDS             1146..1616
                     /locus_tag="VF686_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8661766.1"
                     /translation="MSDSQRFPVAAHTLAYLAHKGAFSPADAAASAVLAASIPTNPVV
                     VRRVTALLAKAGLIATRPGASGGAWLLHRPEDISLETVLRAVNGCAHLGSPPAGAKGC
                     PVSEHIPRQVAKALTAADEAAGAALSKITIADLLDHDPASLSAFAPHASCPVAA"
     gene            1618..>2057
                     /locus_tag="VF686_07860"
     CDS             1618..>2057
                     /locus_tag="VF686_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661767.1"
                     /translation="MSLPSRPRPVVLVTGASAGIGAALARVYAEHGWDLVLTARREAS
                     LKALAEELSHTGAATTVLTEDLADDGAPRRLYDALQDRGLHIDGLVNNAGFSRTAGFL
                     NTDPTAHAAMIRVMLAAPVELARLFAPAMVERGYGRILNVASLAG"
BASE COUNT          329 a          750 c          688 g          290 t
ORIGIN      
        1 gccgacggca ccatgctcca gaacggattt tccgccgccc cgggatgcga gaggatcagg
       61 gcgccctgac ccagatagtt cagggtcagg cagggcagga cgaaggcgac ccatccggcg
      121 cggatgggcg cctttccgaa atggcccatg tcggcgtaga gcgcctcggc cccggtcacg
      181 gcgaggaata cgctgcccag aatgacgaag ccgaggaagc cgtcgttgat cagcagcatc
      241 acgccgtagt ggggcgacag ggcccgcagg atggagacgt cgtcgaagat gtggatcagg
      301 cccaacacgc ccagggacag gaaccagatg gcggtgatcg gcccgaagaa ccgcgccaga
      361 ccggccgtgc cccgcgcctg caccaggaac agggcgatca gaatgcccgc cgagacgggc
      421 acgatgaagg gatccagctt gccgccgacg cccggcgcgt ccttgacgcc ctcgatggcc
      481 gaaaggaccg agatcgcggg ggtgataatg ccgtcgccgt agaacagcgc cgcgccgcag
      541 acgccaagaa tgaagatcag gccgctgcgc cggcccaccg catgggtggc cagggccatc
      601 agggccaggg tgccgccctc gcccttgttg tccgcccgca tcaggaacat gacgtatttg
      661 aacgtcacca cgatcatcag ggcccagaag gccagcgaga ccacgccgac gatcgccaga
      721 tcgccgccca cgccgccctg ggcgtggccg atcgcctcgc gcagggcgta cagcgggctg
      781 gtgccgatgt cgccgaacac gacgccgatg gcgccgacga tcaggcccca tttgccggcc
      841 ttgacgtgac cgccttcgcc ggccgccgcg gggtgcggat gggccggggc gacggatttg
      901 tcggaggggg tctgggagtt tgaggcggcg gcgccagcgc cgtcttcacc ttggggagag
      961 gccccggcca tgaagttcct ccgggacccc gcacagcgcg gttcccatca aaagagcggc
     1021 gagcctttag gctctttcat tcggcggttc aatcgtgctg cgggtgcgaa ggttgcaacg
     1081 cccccggcct tgaagatgtc cggtttgtga aacagcctcg cagcacctat cttggaggct
     1141 gcaccatgtc agacagccaa cgctttcccg tcgccgccca caccttggcc tatctggccc
     1201 acaagggcgc cttctcgccc gccgacgcgg cggcgagcgc ggttctggcc gcctccatcc
     1261 cgaccaatcc cgtcgtcgtg cgccgcgtga ccgccctgct ggccaaggcc gggctgatcg
     1321 ccacccgtcc cggcgcctcg ggcggagcct ggctgctgca ccggcccgag gacatctcgc
     1381 tggaaaccgt gctgcgcgcc gtcaacggct gcgcccacct cggctcgccg cccgccggtg
     1441 ccaagggctg cccggtcagc gaacacatcc cccgtcaggt cgccaaggcc ctgacggccg
     1501 ccgacgaggc cgccggcgcg gccctgtcga agatcaccat cgccgatctg ctggatcacg
     1561 atccggcgtc gctgtccgcc ttcgccccgc acgcatcctg cccggtcgcc gcctgagatg
     1621 agcctgccct cgcggccgcg tccggtcgtg ctggtcaccg gggcgtccgc cggcatcggg
     1681 gccgccctgg cccgggtcta tgcggagcat ggctgggatc tggtcctgac ggcccggcgg
     1741 gaggcttcgc tgaaggccct ggccgaagag ctgtcccaca ccggcgccgc gaccaccgtc
     1801 ctgaccgaag acctcgccga cgacggcgcg ccccgacgcc tgtacgacgc cctgcaggac
     1861 cggggcctcc atatcgacgg tctggtcaac aacgccggct tcagccgcac ggcgggcttt
     1921 ctcaacaccg acccgaccgc ccacgccgcc atgatccgcg tcatgctggc cgcgccggtc
     1981 gaactggccc ggctgtttgc gccggccatg gtcgagcgcg gttacggccg catcctcaac
     2041 gtcgcgtcgc tggccgg
//
LOCUS       DASVVN010000488         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_518393,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000488 DASVVN010000000
VERSION     DASVVN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518393"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VF686_07865"
     CDS             <1..739
                     /locus_tag="VF686_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX8661768.1"
                     /translation="ALFNYGAYTSFRVEDGGVRGLELHLARLDASAVELFGEAVGEER
                     LRDLMRGALGDRTEAWLRVSLFSPDIWMREASWRGRPKVMIGVFNPPPPLAKALRVQI
                     QTYAREAAHLKHAATFGLMRARRAARDAGFDDALFADATGQISEGSLWNIGFLSGNDI
                     VWPRAPMLAGVAQALIERGLGQAGLAGRAARVHVDDLRNFDGAFICNSATPACAVTTI
                     GEHAFATDTAMIDRLTAAWASNPVQPI"
     gene            788..1270
                     /gene="coaD"
                     /locus_tag="VF686_07870"
     CDS             788..1270
                     /gene="coaD"
                     /locus_tag="VF686_07870"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX8661769.1"
                     /translation="MRIGLYPGTFDPVTNGHMDIIGRAVKLVDKLVIGVARNDDKGPL
                     FRTDERVEMVRAEVAHLGDKVEVRAFSTLLMHFAEELDASIIVRGLRAVADFEYEFQM
                     TAMNQRLNADIETVFLMADPRHQAIASRLVKEIARLDGAIDSFVSPAVAKAVRDKVKG
                     "
     gene            1272..2138
                     /locus_tag="VF686_07875"
     CDS             1272..2138
                     /locus_tag="VF686_07875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8661770.1"
                     /translation="MSLRIATIGVFSMALLAAGLPAAAQPPAQTPAAEWRAIAPENLL
                     VIDTAKGRIVVELDPVVAPLSVARIRTLADQGFYDGLKFHRVIPGFMAQTGDPLGTGA
                     GGSELPDVAGEFQFRRGRDLGFVNLIPAPTGQLGIAGSMAVLTQPDAQMMVTADFKTA
                     AQALFCPGVAGMARNQDPNGANSQFFLMTGPNENLNGLYTPFGRVLSGLDVVKALKAG
                     NEANNGQVTDPDVMTQVRTAAAMPEGQRPTARVMTPRSPAFAALVEQKRTELGTRFTV
                     CDIQPPAEVTGG"
     gene            2161..>2528
                     /locus_tag="VF686_07880"
     CDS             2161..>2528
                     /locus_tag="VF686_07880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8661771.1"
                     /translation="MRQVLGLAALSVLMAGAANAQDSGGTPPPPPPPTPATTVAAPEW
                     RTATAENLLVIDTTKGRVIVELTPEVAPLHVERIKLLARQGFYDGVPWHRVIDWFMAQ
                     TGDPLGNGEGQSPYPDLKAE"
BASE COUNT          428 a          842 c          865 g          393 t
ORIGIN      
        1 cgcgctgttc aactacggag cctacacctc ctttcgggtc gaggacgggg gcgtgcgcgg
       61 gctggaactg catctggcgc ggctggacgc ctcggcggta gagctgttcg gcgaagcggt
      121 cggcgaagaa cggttgcgcg atctgatgcg cggcgccttg ggcgaccgga ccgaagcctg
      181 gctgcgagtc agtctgttct cgcccgacat ctggatgcgc gaggcgagct ggcgaggccg
      241 gcccaaggtc atgatcgggg tcttcaatcc accgcctccg ctggccaagg cgttgcgcgt
      301 gcagatccag acctacgcgc gggaggcggc gcatctgaag cacgccgcca cgttcggcct
      361 catgcgcgcg cgccgcgccg cgcgcgacgc cggcttcgac gacgccctgt ttgccgatgc
      421 gacaggacag atttcagaag gaagcctttg gaatataggg tttctttctg gaaacgatat
      481 tgtttggccc cgggcgccga tgttggcggg cgtggcacag gcgctgatcg aacgcggctt
      541 gggacaggca gggctggcgg gccgcgccgc gcgggtccat gtggacgatc tgcggaattt
      601 cgacggcgcc ttcatctgca acagcgcgac gccggcctgc gccgtgacca cgatcggcga
      661 gcatgcgttc gcgacggaca ccgcaatgat cgaccgcctc accgccgcat gggcgtcgaa
      721 cccggttcaa ccgatctagg ctgtggcggc ggcggctgct aaaccgctgg aaagcgggga
      781 ggactgcatg cgcatcggcc tgtatccggg cacgttcgat cccgtgacca acggacatat
      841 ggacatcatc ggccgggcgg tgaagctggt cgacaagctt gtcatcggcg tcgcccgcaa
      901 cgacgacaag gggccgctgt tcaggacgga cgaacgcgtc gaaatggtcc gcgccgaggt
      961 cgcccatctg ggcgacaagg tcgaggtgcg ggccttttcc accctgctga tgcatttcgc
     1021 cgaggagctg gacgccagca tcatcgtgcg cggcctgcgc gccgtcgccg acttcgaata
     1081 cgaattccag atgacggcga tgaaccagcg cctcaatgcc gacatcgaga cggtcttcct
     1141 gatggcggac ccgcggcatc aggcgatcgc ctcgcggctg gtcaaggaaa tcgcccgtct
     1201 ggacggcgcg atcgacagtt tcgtcagccc ggccgtcgcc aaggcggtcc gggacaaggt
     1261 caagggatag aatgagcctc cgtatcgcaa ccatcggcgt gttctcgatg gcgctcctcg
     1321 ccgccggcct gcccgcggcg gcccagccac cggcccagac gccggccgcc gaatggcgcg
     1381 ccatcgcgcc ggagaacctg ctggtcatcg acaccgcgaa gggccggatc gtggtcgagc
     1441 tggatccggt cgtcgcgccg ttgtcagtcg cgcgcatccg gaccctcgcc gatcagggct
     1501 tctacgacgg cctgaaattc catcgggtga tcccgggatt catggcccag accggcgatc
     1561 ccctcggcac tggcgcgggc ggcagcgaac tgccggacgt cgccggcgaa ttccagttcc
     1621 ggcgcggacg cgacctgggc ttcgtcaatc tgatcccggc gccgaccggg caactgggca
     1681 tcgccggctc gatggcggtc cttacccagc ccgacgcgca gatgatggtc acggccgact
     1741 tcaagaccgc cgcccaggcc ctcttctgcc ctggcgtcgc cggcatggcc cggaatcagg
     1801 accccaacgg cgccaacagc cagttcttcc tgatgaccgg acctaacgag aacctgaacg
     1861 gcctgtacac ccccttcggg cgcgtgctgt ccggtctgga cgtggtcaag gcgctgaagg
     1921 ccgggaacga ggccaacaat ggtcaggtga ccgatccgga cgtcatgacc caggtccgca
     1981 cggcggcggc catgcccgag gggcagcgcc cgaccgcgcg cgtgatgacg ccgcgcagcc
     2041 cggccttcgc ggccctggtc gaacagaaac ggaccgagct gggaacgcgc ttcacggtct
     2101 gtgacataca gccgcccgcc gaagtgaccg gcggctgatg ttgaaatgag ggggaagacg
     2161 atgcggcagg ttctcggtct cgcggctttg agcgtcctga tggcgggcgc ggcgaacgcg
     2221 caggattcgg gcggcacgcc gcctccgccg cctccgccca cgcccgcaac aacggtcgcc
     2281 gcgcccgagt ggcggacggc gaccgccgag aacctgctgg tcatcgacac caccaagggc
     2341 cgggtcatcg tcgaactgac gcccgaggtg gcaccgctgc atgtcgagcg gatcaagctg
     2401 ctggcgcgac aaggcttcta tgacggcgtg ccgtggcacc gggtgatcga ctggttcatg
     2461 gcccagaccg gcgacccgct tggcaatggc gaaggtcaga gcccgtatcc cgacctgaag
     2521 gccgaatt
//
LOCUS       DASVVN010000489         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_229287,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000489 DASVVN010000000
VERSION     DASVVN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229287"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1130
                     /gene="trxB"
                     /locus_tag="VF686_07885"
     CDS             120..1130
                     /gene="trxB"
                     /locus_tag="VF686_07885"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX8661772.1"
                     /translation="MSTLRTVRVAIIGSGPAGWTAAIYAARALLSPVVIAGLQPGGQL
                     TITTDVENYPGFKDVIQGPWLMEQMRAQAEHVGTEVIEDIVVKADLSQRPFRLTLDSG
                     TEMLAETVIISTGAQAKWLGIESEQKYQGFGVSACATCDGFFYRGKDVIVVGGGNTAV
                     EEALFLTNFASKVTVVHRRDEFRAERILQDRLFSNPKVEVVWDNAIDEILGETNSFGG
                     VNVTGARIKNVKTGETRDITADGVFIAIGHAPSSELFAGQLETKQGGYLMVKPGTAAT
                     AIEGVWAAGDVTDDIYRQAVTAAGMGCMAALEAVRFLAEEDHARAHHPISHKEAEKIG
                     VW"
     gene            complement(1137..1544)
                     /locus_tag="VF686_07890"
     CDS             complement(1137..1544)
                     /locus_tag="VF686_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8661773.1"
                     /translation="MSARILVIEDEALVAMELRFVLEDLGHEVVGTAADARTARRLVQ
                     ETEVDLALVDIHLSDGPTGIDLGRELGQQLGVTVLFMTANPGMVRNGVAGTMGVLSKP
                     TDERSVQTAVDYALGRRAGRPIEYAPPELQLFA"
     gene            complement(1646..>2036)
                     /locus_tag="VF686_07895"
     CDS             complement(1646..>2036)
                     /locus_tag="VF686_07895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8661774.1"
                     /translation="ATLDGRMRIEISGDDAVFDDQAATSVALLFHELATNAAKYGALS
                     VEGGAITLNTRNEGGRFILVWEERGGPHVAGEPSRTGFGSSLATLSVENQLGGRLERI
                     WAPEGLRVTADLPATALSRRRAAQIVA"
BASE COUNT          359 a          684 c          665 g          328 t
ORIGIN      
        1 atgcccagcg ccagaccgac gccgatgaaa ctgcggcctt ccatgtcgtc actccctcag
       61 acctgtgaac aggtggaacc taccatcgca agaagcggaa tcagcgccta aataggggca
      121 tgagcacctt gagaaccgtc cgcgtcgcca tcatcggctc cggccccgcc ggctggaccg
      181 ccgccatcta cgccgcccgc gccctgttga gccccgtcgt catcgccggc ctgcagcccg
      241 gcggccagct gaccatcacc acggatgtcg agaactatcc cggcttcaag gatgtgatcc
      301 agggtccgtg gctgatggag cagatgcgcg cccaggccga gcacgtcggc accgaggtca
      361 tcgaggacat cgtggtcaag gcggacctgt cgcaacggcc gttccgcctg acgctggaca
      421 gcgggacaga gatgctggcc gagacggtga tcatctcgac cggcgcccag gccaagtggc
      481 tggggatcga gagcgagcag aaataccagg gtttcggcgt ctccgcctgc gccacctgcg
      541 acggcttctt ctatcgcggc aaggacgtga tcgtcgtcgg cggcggcaat accgcggtcg
      601 aggaggcgct gttcctgacc aatttcgcgt cgaaggtcac ggtcgtgcac cgccgcgacg
      661 agttccgggc cgagcgcatc ctgcaggacc ggctgttctc caatcccaag gtcgaggtgg
      721 tctgggacaa cgccattgac gagatcctcg gcgagaccaa cagcttcggc ggggtcaacg
      781 tcaccggcgc gcggatcaag aacgtgaaga cgggcgagac gcgcgacatc acggcggacg
      841 gcgtcttcat cgccatcggc cacgcgccgt cgtcagagct gttcgcgggc cagctggaga
      901 ccaaacaggg cggctatctg atggtgaagc cgggcacggc ggcgaccgcc atcgaaggcg
      961 tctgggccgc cggcgacgtg accgacgaca tctaccgcca ggctgtcacg gcggcgggca
     1021 tgggctgtat ggcggcgctg gaggcggtcc gcttcctcgc cgaggaagac cacgccaggg
     1081 cgcatcatcc catctcgcac aaggaagcgg aaaagatcgg cgtctggtag cccgcgtcag
     1141 gcgaacagct gcagctcggg cggggcgtat tcgatcggac gtccggcccg gcggcccagg
     1201 gcgtagtcga ccgcggtctg cacggaccgc tcatcggtcg gctttgacag cacgcccatc
     1261 gtgcccgcca cgccgttgcg caccatgccg gggttggcgg tcatgaacag gacggttacg
     1321 cccagctgct gacccagttc gcggcccaga tcgatacctg tgggcccgtc agacaggtgg
     1381 atgtcgacga gggccaggtc gacctcggtt tcctgcacca gtcgccgcgc cgtgcgcgcg
     1441 tcagcagccg tcccgaccac ctcatggccg agatcttcca gtacgaagcg cagctccatg
     1501 gccaccaatg cttcgtcttc aatgaccagg atacgcgcac tcatgggatt tcagacgctt
     1561 ctgcctctct tgttcagcgg ccgcagtatg cggacagact ggtgaaccaa cgctaactgt
     1621 tatgtagggt tgcggaccaa cgacgttatg cgacaatttg tgcggcgcgg cgtcgtgaca
     1681 gggctgtggc cggaaggtcg gcggtgaccc gcagtccctc gggcgcccag atccgctcca
     1741 gccgcccgcc cagctggttt tcgaccgaca gggtcgccag cgacgagccg aagccggttc
     1801 gggacggctc gcccgccaca tgcggtccgc cccgctcttc ccagaccagg atgaaccggc
     1861 cgccctcgtt gcgggtgttc agggtaatgg cgccgccctc gaccgacagg gcgccgtatt
     1921 tcgccgcgtt ggtggccagt tcgtggaaca gcagggccac cgaggtcgcc gcctgatcgt
     1981 cgaacactgc atcgtccccg ctgatttcga tccgcatccg accgtccagg gtcgca
//
LOCUS       DASVVN010000490         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_177884,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000490 DASVVN010000000
VERSION     DASVVN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177884"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VF686_07900"
     CDS             complement(<1..1384)
                     /locus_tag="VF686_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8661775.1"
                     /translation="MAYKAPVRDLTFILDEVLEIDRYSNQPGFEDVSADLVAQILEEG
                     AKFAEEVIAPLNRVGDQEGCKWDNGKVTGPKGWKEAYQAMVAAGWPALSALPEHGGQG
                     MPSVVAMAFGQMTAAASAAFSMYPGLTAGAYWGLNANGSDELKAKYLPKMATGEWGGT
                     MNLTEPQCGTDLGLIRTKAVPNGDGSYNITGQKIWISSGEHDFTDNIIHLVLARIEGA
                     PAGIKGISLFVVPKVFVNDDGSLGERNEGAACAGLEHKMGIHGNATCVMNYDGARGWL
                     VGQENRGLNAMFVMMNEARLAVGVQGLAQSEVAYQNAVAYAKDRLQGRALTGAKFPDK
                     AADPIIVHPDVRRTLLSIKSFNEAARALVVWTALNSDIAHRSSDEAERRTADDYMGLM
                     TPLVKGVLTDLGFDNAVKAQQMFGGHGYIEEWGMSQFVRDARIAMIYEGANGIQAMDL
                     VGRKLGKDGGR"
     gene            complement(1387..>1587)
                     /locus_tag="VF686_07905"
     CDS             complement(1387..>1587)
                     /locus_tag="VF686_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8661776.1"
                     /translation="AQIAALKQQREDLDAAIETAEEACAVMEKKLGEFRPDLLPGAEE
                     YADLLKARLNPDPHHQPFKARA"
BASE COUNT          230 a          535 c          523 g          299 t
ORIGIN      
        1 cgcgccctcc gtccttcccc agcttgcggc ccacgagatc catggcctgg atgccgttgg
       61 ctccctcgta gatcatggcg atgcgggcgt cgcgcacgaa ctgcgacatg ccccattcct
      121 cgatatagcc gtggccgccg aacatctgct gcgccttcac ggcgttgtcg aagccgagat
      181 cggtgagcac gcccttcacc agcggggtca tgaggcccat gtagtcgtcg gcggtccggc
      241 gctcggcctc gtccgaggag cggtgcgcga tgtcgctgtt gagcgcggtc cagaccacca
      301 gcgcccgcgc ggcctcgttg aacgacttga tggacagaag cgtgcggcgc acgtccggat
      361 gcacgatgat cggatcggcc gccttgtcgg ggaatttcgc gccggtgagc gcccggccct
      421 gaagccggtc cttcgcgtag gccaccgcgt tctggtaggc gacctcggat tgcgccagcc
      481 cctgcacgcc cacagccagc cgcgcctcgt tcatcatcac gaacatggcg ttgaggccgc
      541 ggttctcctg cccaaccagc cagccccgcg cgccgtcata attcatgacg caggtggcgt
      601 tgccgtggat gcccatcttg tgctcgaggc cggcgcaggc ggcgccctcg ttgcgctcgc
      661 ccagcgagcc gtcgtcgttg acgaagacct tgggcaccac gaacaggctg atgcccttga
      721 tgccggcggg cgcgccttcg atgcgggcca gcaccagatg gatgatgttg tcggtgaagt
      781 cgtgctcacc cgacgagatc cagatcttct ggccggtgat gttgtagctg ccgtcgccgt
      841 tgggaacggc cttggtgcgg atcagaccca ggtccgtgcc gcactgcggc tcggtcaggt
      901 tcatggtgcc gccccattcg ccggtggcca tcttgggtag atatttcgcc ttcagctcgt
      961 ccgagccgtt ggcgttcagg ccccagtagg cgccggccgt cagacccgga tacatcgaga
     1021 aggcggcgct ggcggcggcc gtcatctggc cgaacgccat ggccacgacc gagggcatgc
     1081 cctgaccgcc atgctcgggc agggccgaca gggcgggcca gccggcggcg accatggcct
     1141 gataggcttc cttccagccc ttgggtccgg tgaccttgcc gttatcccat ttgcagcctt
     1201 cctgatcgcc gacgcggttc aggggagcga tgacctcctc cgcgaacttg gcgccctcct
     1261 ccaagatctg ggccaccaga tcggcggaga cgtcctcgaa accgggctgg ttcgaatagc
     1321 ggtcgatctc cagcacctcg tccagaatga acgtcaggtc gcggacaggc gccttgtagg
     1381 ccatgttcag gctctcgctt tgaagggctg gtggtggggg tcgggattca gccgcgcctt
     1441 cagcaggtcg gcatactcct cggcgccggg cagaaggtcg gggcggaact cgcccagctt
     1501 cttctccatc acggcgcagg cttcctcggc cgtctcgatg gcggcgtcga ggtcttcgcg
     1561 ctgctgtttc agcgcggcga tctgggc
//
LOCUS       DASVVN010000491         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_229425,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000491 DASVVN010000000
VERSION     DASVVN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229425"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VF686_07910"
     CDS             complement(<1..919)
                     /locus_tag="VF686_07910"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8661777.1"
                     /translation="MKFTLSWLKEHLETDADVAAVAEAMTMAGLEVEEVHDSIAALAP
                     FTVARIVSAERHPNADRLQVCQVETVDGMKEIVCGAPNARAGLTTIYAPIGAFVPGLG
                     VTLVEKPVRGVVSNGMLCSASELELADESDGILELADDLTVGTPAAGVFGAEPVIDFE
                     VTPNRPDWLGVAGIARDLAAASLGQLKTPSVVPVAGGYPSPVTVRIEAPEMCPAYAGR
                     MIRGVRNGPSPEWLQQRLTAIGLRSISRLVDVTNLLAYDRCRPTHVYDAAKLVGTEIV
                     VRGGQVARDTGEHEHLIALDGKTYTIDPAI"
     gene            complement(916..1347)
                     /locus_tag="VF686_07915"
     CDS             complement(916..1347)
                     /locus_tag="VF686_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661778.1"
                     /translation="MTDQIPNPSADEAAGRALVARTTFEKEAIWLPSFAVQHWNAGQM
                     FIDGKTFTDCLIEGPGVMAVMNGTHFDSCAMGTTTDVRNLMYRPVSRTKLAGVIGMSN
                     CRFVRCRFVQIGFTGQDELVDQLIEGVTPMSSLKPPSEPAA"
     gene            complement(1380..1775)
                     /locus_tag="VF686_07920"
     CDS             complement(1380..1775)
                     /locus_tag="VF686_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661779.1"
                     /translation="MTDQPVSPAATTRSLDPLSTEFTGGTFALIDLYRASVAAGGGAV
                     IEGRAFRDCQIHGPALMLVLENVHFDSTNFGPTGGDMRNMLFKPMGDVGIGAIPVRNC
                     TFTNCRFDTLGLTGNDDFLQMLIENVATV"
     gene            complement(1775..2851)
                     /gene="pheS"
                     /locus_tag="VF686_07925"
     CDS             complement(1775..2851)
                     /gene="pheS"
                     /locus_tag="VF686_07925"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8661780.1"
                     /translation="MTDLARLELDLINAIGSAETAAAVEELRVAALGKSGSISGLLKG
                     MGALSPDERREQGPVINGLRDRVSSALAARKAELEAVELDARLKNEHVDLTLPARPRR
                     KGSVHPTMQVMDEMIALFADMGFAVAEGPDIEDDFHNFTALNFPPKHPAREMHDTFFL
                     RPDPDTGERKVLRTHTSPVQVRTMMTQKPPIRIIAPGRTFRKDSDATHTPMFHQVEGL
                     VIDRNIHMGHLKWTLETLVARFFEVDGVDARFRPHHFPFTEPSAEMDIRCDRSGGELK
                     LNTGDSWMEILGCGMVHPNVLRNCGIDPDEYQGFAFGMGVDRLAMLKYGIPDLRPMFD
                     ADTRWLAHYGFSAFAAPNPASGLS"
     gene            complement(2907..3851)
                     /locus_tag="VF686_07930"
     CDS             complement(2907..3851)
                     /locus_tag="VF686_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8661781.1"
                     /translation="MFARSILAALAACLLSGPAWAQDRAVAVTFDDLPYQAADAELCD
                     PARAMALTETFVGMLRPLDTRAAVFVNESKVCEARRATLLPALLDVWLDAGLDLGNHT
                     ATHINIHRNTVEAYLADVDAGAPVSRAVLAARGRELRFFRHPFLFTGETPEKKTAIAA
                     GLAERGYVVAPVTIDNNDWMFAALYRDAEAAGDEALKTRIGEAYVAHMAAVLDHWEPY
                     SVELSGGREPAQVLLLHANTLNRDRYPQIHALYLSRGYRFVTLDEALTDPVYAHADEY
                     VRANGVSWLHRWTFSEGRPIRWEPEPPKWIVDAYETRD"
     gene            complement(3943..4302)
                     /gene="rplT"
                     /locus_tag="VF686_07935"
     CDS             complement(3943..4302)
                     /gene="rplT"
                     /locus_tag="VF686_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX8661782.1"
                     /translation="MARVKRGVTSHAKHKKVLKQAKGFYGRRKNTIRTAKAAVDRAGQ
                     YAYRDRRNKKRSFRALWIQRINAAARLEGFTYSQFIHGLGEAGIELDRKVLASMAADE
                     AAFKAIADKVRDALKTA"
     gene            complement(4410..4607)
                     /gene="rpmI"
                     /locus_tag="VF686_07940"
     CDS             complement(4410..4607)
                     /gene="rpmI"
                     /locus_tag="VF686_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX8661783.1"
                     /translation="MPKLKTKSGAKKRFKVTGTGKVMSAHAGKRHRLISHNSKYIRQN
                     RGTSVMADADAKKIKSYMPYA"
BASE COUNT          720 a         1490 c         1595 g          839 t
ORIGIN      
        1 aaatggccgg gtcgatcgta taggtcttgc cgtcgagcgc gatcagatgc tcgtgctcgc
       61 ccgtgtcccg ggccacctga ccgccgcgca cgacgatctc ggttccgacc agcttggccg
      121 cgtcatagac gtgggtgggc cggcagcggt cgtaggcgag cagattggtg acatcgacca
      181 gccggctgat cgagcgcagg ccgatggccg tcagccgctg ctgcagccat tccggcgagg
      241 gcccgttcct gaccccgcgg atcatccgtc cggcgtaggc ggggcacatc tccggcgcct
      301 cgatgcgcac cgtgacgggc gagggatagc cgcccgccac cgggaccacc gacggggtct
      361 tcagctgacc caggctggcc gcggcgagat cgcgcgcgat gcccgccacg ccgagccagt
      421 cgggccggtt cggggtcacc tcgaaatcga ttaccggctc ggcgccgaac acaccggccg
      481 ccggcgttcc gacggtaaga tcgtcggcca gttccagaat gccgtcgctc tcgtcagcca
      541 gttcgagctc ggaggccgaa cacagcatgc cgttcgagac cacgccgcgc accggcttct
      601 cgaccagggt cacccccaag ccaggtacga aggcgccgat cggcgcatag atggtggtca
      661 ggcccgcgcg cgcgttaggc gcgccgcaga cgatctcctt cattccgtcg accgtctcga
      721 cctgacagac ctgcaggcgg tcggcgtttg gatggcgttc ggccgagacg atccgggcga
      781 ccgtgaaggg ggccagggcg gcgatcgaat cgtggacctc ctcgacctcc agcccggcca
      841 tggtcatggc ctcggccacg gccgcgacgt cggcgtccgt ctcgaggtgt tccttcagcc
      901 aggagagggt gaatttcacg ccgcgggctc cgagggaggc ttcaggctgc tcatcggggt
      961 cacaccctcg atcaactggt ccaccagttc gtcctggccg gtgaagccga tctggacgaa
     1021 ccggcagcgg acgaaacggc agttggacat gccgatcacg ccggccagct tggtccggct
     1081 taccgggcgg tacatcaggt tccggacatc cgtcgtcgtg cccatggcgc agctgtcgaa
     1141 atgcgtgccg ttcatgacgg ccatcacgcc cggcccctcg atcaggcagt cggtgaaggt
     1201 cttgccgtcg atgaacatct ggccggcgtt ccagtgctgg accgcgaagg acggaagcca
     1261 gatggcttcc ttttcgaagg ttgtgcgcgc gacgagtgcg cggccggcgg cttcgtcagc
     1321 agacgggttc gggatctggt cagtcatgtt tcagtctttc gattcagttc cgggcgcgat
     1381 cagacggtcg cgacattctc gatcagcatc tggagaaagt cgtcgttgcc cgtcagcccc
     1441 agggtgtcga aacggcagtt ggtgaaggtg cagttgcgca ccggaatcgc tccgattccg
     1501 acatcgccca tgggcttgaa cagcatgttt cgcatgtcgc cgccggtcgg gccgaaattg
     1561 gtgctgtcga aatggacgtt ttcgagcacc agcatcagcg cgggaccgtg gatctggcag
     1621 tccctgaacg cgcgcccctc gatcaccgct ccaccgccgg ccgcgacgct ggcgcggtag
     1681 agatcgatca gggcgaaggt tccgccggtg aactcggtcg aaagcggatc cagcgagcgg
     1741 gttgtcgctg ccggggaaac gggttggtcg gtcatcagga cagtccgctt gcagggttgg
     1801 gcgcggcgaa ggccgagaag ccgtagtggg ccagccaccg ggtgtcggcg tcgaacatcg
     1861 ggcgcaggtc ggggatgccg tatttcagca tggccagacg gtccacgccc atgccgaagg
     1921 cgaagccctg gtattcatcg gggtcgatgc cgcagttgcg cagcacgttc gggtgaacca
     1981 tcccgcagcc gaggatctcc atccagctgt cgccggtgtt cagcttcagc tcgccgcccg
     2041 agcggtcgca gcggatatcc atctcggccg acggctcggt gaacgggaaa tgatgcgggc
     2101 ggaagcgggc gtcgacgccg tccacctcga agaagcgggc gaccaaggtc tcgagcgtcc
     2161 acttcaggtg gcccatgtgg atgttgcggt cgatgaccag cccttcgacc tgatggaaca
     2221 tcggcgtgtg ggtggcgtcg ctgtccttac ggaaggtccg gccgggcgcg atgatccgga
     2281 tcggcggctt ctgcgtcatc atggtccgca cctgcaccgg gctggtgtgg gtgcgcagga
     2341 ccttgcgctc gccggtgtcc gggtcgggcc tgaggaagaa ggtgtcgtgc atctcccgcg
     2401 ccgggtgttt gggcgggaag ttcagggcgg tgaaattgtg gaagtcgtcc tcgatgtccg
     2461 ggccctcggc caccgcgaag cccatgtcgg cgaacagggc gatcatctcg tccatcacct
     2521 gcatggtcgg gtgcaccgag cccttgcggc gcggacgggc cggcagggtc aggtcgacgt
     2581 gttcgttttt caggcgggcg tcgagttcga cggcttccag ctcggccttg cgggcagcca
     2641 gggccgacga cacccggtcc cgcaggccgt tgatgaccgg accctgttcg cgccgctcgt
     2701 cggggctgag cgcccccatg cccttcagca ggccggagat ggagccggac ttgcccagcg
     2761 ccgccacgcg cagttcctcg accgccgcgg cggtttccgc cgagccgatg gcgttgatca
     2821 ggtcgagttc gagtctggcg aggtcggtca tggtcgcagt cgtctagcgg gcgcgggccg
     2881 tttcgtcacc tccggccttc cggaagtcag tcgcgggtct cgtaggcgtc cacaatccac
     2941 ttcggcggct ccggctccca acggatcggc cggccctcgc tgaaggtcca gcgatgcagc
     3001 cacgagacgc cgttggcgcg cacgtattcg tccgcatggg cgtagaccgg gtcggtcagg
     3061 gcctcgtcca gggtcacgaa gcggtagcct cgtgacaggt agagggcgtg gatctgaggg
     3121 tatcgatcgc ggttcagcgt attggcgtgc agcagcagca cctgcgccgg ctcgcgtcct
     3181 ccgctcagtt cgacgctgta gggctcccag tggtccagca ccgcggccat gtgggccaca
     3241 taggcctcgc cgatgcgcgt cttcagcgcc tcgtcgccgg ctgcctcggc gtcgcggtac
     3301 agggctgcga acatccagtc gttgttgtcg atggtcaccg gcgcgacgac atagccgcgc
     3361 tccgccagcc ccgcggcgat ggcggtcttc ttctccggcg tctcgccggt gaacagaaac
     3421 ggatggcgga agaaccgcag ttcgcgccct cgcgccgcga gcaccgcccg gctgacgggc
     3481 gcccccgcgt cgacatcggc cagatacgcc tccaccgtgt tgcgatgaat gttgatgtgg
     3541 gtcgcggtgt ggttgccaag gtcgagcccg gcgtcgagcc agacatccag cagcgccggg
     3601 agcagcgtcg ctcggcgagc ctcgcagacc ttgctctcgt tgacgaacac cgccgcccgg
     3661 gtgtccagcg gccgcagcat gccgacaaag gtctcggtga gcgccatcgc ccgcgccgga
     3721 tcgcaaagct ctgcgtcggc cgcctgatat ggcaggtcgt caaaggtgac ggcgaccgcc
     3781 cggtcctgcg cccatgcagg ccccgacaac aggcaggcag cgagcgccgc gaggatcgat
     3841 cgtgcaaaca tccgtcgagc ctagccgctc agggccgaaa cgaaaaacgc ccgctccgga
     3901 gaccggagcg ggcgtttcga aagctgtgcg tcagacctgc aatcaggcgg tcttcagcgc
     3961 gtcccggacc ttgtcggcga tcgccttgaa ggcggcttcg tcggcggcca tcgaggccag
     4021 gaccttgcgg tccagttcga tgccggcttc gcccaggccg tggatgaact gcgaataggt
     4081 gaagccttcc agacgcgcgg cggcgttgat gcgctggatc cacagggcgc ggaacgagcg
     4141 cttcttgtta cggcgatcgc ggtaggcgta ttgcccggcg cggtcgaccg ccgccttggc
     4201 ggtgcggatg gtgttcttgc ggcggccgta gaaacccttg gcctgcttga gaaccttctt
     4261 gtgcttggcg tgggacgtta cgccccgttt gacgcgagcc atgtcgctat ccttcttttg
     4321 ggtcccctac cgcctcgcgc tcggcgctcg gctacgtgag gggaagcgtt caaattctgg
     4381 tggagatcag atgcaggtcc gcgcgccgat caggcgtacg gcatgtagga tttgatcttc
     4441 ttggcgtcgg cgtcggccat gaccgaggtg ccgcggttct ggcggatgta tttgctgttg
     4501 tggctgatca agcggtgacg cttgcccgca tgggccgaca tcaccttccc ggtaccggta
     4561 actttgaagc gctttttagc gcccgacttg gtcttcagct tgggcatttt gctctcctat
     4621 ttccggcatg gcgacaggcg caag
//
LOCUS       DASVVN010000492         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_44723, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000492 DASVVN010000000
VERSION     DASVVN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44723"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF686_07945"
     CDS             <1..618
                     /locus_tag="VF686_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX8661784.1"
                     /translation="RTMNRMNDTIPGAKVTGRIEMDGDDINAKAVDPVLLRARVGMVF
                     QKPNPFPKTIYENVAYGPRIHGLAASKGELDSVVEASLKRAGLWEEVADRLHSPGTGL
                     SGGQQQRLVIARAIAVEPEVILMDEPCSALDPIATAKIEELIDELRERYSIVIVTHSM
                     AQAARVSQRTAFFHLGRLIETGDTEDIFTNPRERRTLDYITGRFG"
     gene            618..1346
                     /gene="phoU"
                     /locus_tag="VF686_07950"
     CDS             618..1346
                     /gene="phoU"
                     /locus_tag="VF686_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX8661785.1"
                     /translation="MNQHTVKAYGDELNQLTAEVARMGGLAEAQVADAVDSVARRDIA
                     LARAVVERDGRLDAMHRDIERKAIRLIALRQPVASDLRRTLASMKLAVDLERTGDLAK
                     NIAKRGLILAEGDPMPPLTRSIERMGKLVSMRLRDVLDAYAAAEIDRAIAVWNTDDEV
                     DEHYNALFRELLTYMMGDPRTITAGTHLLFMAKNLERIGDHATNIAETIYYEITGQEF
                     TGERPRWNPDPLAPTDDTDPTTTA"
     gene            1361..2050
                     /gene="phoB"
                     /locus_tag="VF686_07955"
     CDS             1361..2050
                     /gene="phoB"
                     /locus_tag="VF686_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX8661786.1"
                     /translation="MQPYVLVMEDEDALATLLSYNLEKEGYRVVVAADGEEGMLQIDE
                     RLPDLVLLDWMLPKLSGIEVCRRIRGRAETRNLPIIMLTARGEESDRIRGLDTGADDY
                     LTKPFSMSELIARIRAVLRRIRPGLADDRINHGDIVIDRVAHRVRRAGNEIHLGPTEF
                     RLLDHLMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHVGRLRKALNVEGTVNPIRTV
                     RSAGYSLDLGG"
     gene            complement(2026..2376)
                     /locus_tag="VF686_07960"
     CDS             complement(2026..2376)
                     /locus_tag="VF686_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661787.1"
                     /translation="MLPLLLMLVSDPLATGAPVPPTNPEIRLRVIDASAAPRCDGGGY
                     QLIDQPSGSRDLRPDMTYRPDGEVRRYLLLDRTVRGCPAPISYAVPGGQDGFIRDLGR
                     TTPPVRVQPPRSSE"
     gene            2510..>2799
                     /locus_tag="VF686_07965"
     CDS             2510..>2799
                     /locus_tag="VF686_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8661788.1"
                     /translation="MRRRTFLSTLPAGAILAGAGATAAAAPAAPPRQTTPADPFAGVG
                     PGNRNSGVQFVSRSTVWGTHGAAATAHPRATLIGIDTLRRGGSAIDAAIAINA"
BASE COUNT          480 a         1068 c          868 g          383 t
ORIGIN      
        1 cggaccatga accgcatgaa cgacaccatc cccggcgcca aggtcaccgg ccgcatcgag
       61 atggacggcg acgacatcaa tgcgaaggcg gtcgaccccg tcctgctgcg cgctcgcgtt
      121 ggcatggtgt tccagaagcc gaaccccttc cccaagacca tctacgagaa cgtcgcctac
      181 ggcccccgca tccacggcct ggccgcgtcc aagggcgagc tggacagcgt ggtcgaggcc
      241 tcgctgaaac gcgccggcct atgggaagag gtcgccgacc gtctgcactc gccgggcacc
      301 ggcctgtccg gcggccagca gcagcgtctg gtgatcgccc gcgccatcgc cgttgagccc
      361 gaagtcatcc tgatggacga gccctgctcg gccctcgacc cgatcgccac ggccaagatc
      421 gaggagctga tcgacgagct tcgcgagcgc tactccatcg tcatcgtcac ccattcgatg
      481 gcccaggccg cgcgcgtcag ccagcgcacc gccttcttcc acctgggccg cctgatcgag
      541 accggcgaca ccgaggacat cttcaccaat ccccgcgagc ggcgcacgct ggactacatc
      601 accggaagat tcggctgatg aaccagcaca ccgtcaaggc ctacggcgac gaactgaacc
      661 agctgaccgc cgaggtcgcc cgcatgggcg gtctggccga ggcccaggtc gccgacgccg
      721 tcgattccgt agcccgccgc gacatcgccc tggcccgcgc cgtggtcgag cgcgacggcc
      781 ggctggacgc catgcaccgc gacatcgagc gcaaggccat ccgcttgatc gccctgcgcc
      841 agcccgtcgc ctccgacctg cgccgcaccc tcgcctcgat gaagctggcg gtcgatctgg
      901 agcgcaccgg cgacctggcc aagaatatcg ccaaacgcgg cctgatcctc gctgagggcg
      961 accccatgcc gcccctgacc cgctccatcg agcggatggg caagctggtc tccatgcgcc
     1021 tgcgcgacgt gctcgacgcc tacgccgccg ccgagatcga ccgcgccatc gccgtctgga
     1081 acaccgacga cgaggtcgac gagcactaca acgccctgtt ccgcgaactg ctgacctaca
     1141 tgatgggcga cccgcgcacg atcacggcgg gcacccacct gctgttcatg gccaagaacc
     1201 tcgaacgcat cggggatcac gccaccaaca tcgccgaaac catctactac gagatcacgg
     1261 gtcaggaatt cacgggcgag cgtccccgct ggaaccccga cccgctcgcg ccgaccgacg
     1321 acaccgaccc gacgacgacc gcctgaccgg agagttcgac gtgcagcctt atgttctggt
     1381 gatggaagac gaggacgccc tggcgacctt gctctcgtac aatctcgaaa aggagggcta
     1441 tcgcgtcgtg gtcgccgccg acggcgagga gggcatgctc cagatcgacg agcgcctgcc
     1501 cgatctggtc ctgctggact ggatgctgcc caagctgtcg ggcatcgagg tctgccgccg
     1561 catccgcggc cgggcagaga cgcgcaacct gccgatcatc atgctgaccg cgcgcggcga
     1621 ggaaagcgac cgcatccgcg ggctcgacac cggcgccgac gactatctga ccaagccctt
     1681 ctcgatgagc gagctgatcg cccgcatccg cgccgtcctg cgccgcatcc gtccgggtct
     1741 agccgacgac cgcatcaacc acggcgacat cgtcatcgac cgtgtggcgc accgcgtccg
     1801 ccgcgccggc aacgagatcc acctcggccc caccgaattc cgcctgctcg accatctgat
     1861 gcagcacccc ggccgcgtgt tcagccgcga acagctgctg gacgccgtct ggggctcgga
     1921 cgtctatgtc gaggcgcgca ccgtcgacgt ccacgtcggc cggctgcgca aggcgctgaa
     1981 cgtcgagggc acggtcaatc cgatccggac cgtgcggtcg gcgggctatt cgctcgatct
     2041 gggcggctga acccgaaccg gcggcgtggt gcgtccgagg tcccggatga agccgtcctg
     2101 cccgccggga acggcgtatg agatcggcgc cgggcagccc ctgaccgtcc gatccagcag
     2161 cagatagcgc cgcacctcgc catcgggcct gtaggtcatg tcgggccgca ggtcgcgaga
     2221 cccggacggc tgatcgatca actgataccc gccgccgtcg cagcgcggcg cggccgaggc
     2281 gtcgatgacc cgcagacgga tctcgggatt tgtcggcgga acgggcgcgc ccgtcgccag
     2341 gggatctgaa accagcatca gcagaagcgg cagcatgaca acgtctccgt gcgcgcgcag
     2401 tctacgcctg atcgcggcgc acgtcatgcg atgggcgatg agaaaggatc ggacttcccc
     2461 ctgctgacga agcagctagg gtcccctcct cagttcaggg agcccgccca tgcgccgccg
     2521 caccttcctc tcgaccctgc ccgcaggcgc catcctcgcc ggcgccggtg cgacggctgc
     2581 ggccgccccg gccgcccctc cccgccagac cacgcccgcc gaccccttcg ccggcgtcgg
     2641 ccccggcaac cgcaacagcg gcgtccagtt cgtcagccgc tcgaccgtct ggggcaccca
     2701 cggcgccgcc gccacggccc atccccgcgc caccctgatc ggcatcgaca ccctgaggcg
     2761 cggcggctcg gccatcgacg cggccatcgc catcaacgc
//
LOCUS       DASVVN010000493         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_202361,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000493 DASVVN010000000
VERSION     DASVVN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202361"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..826)
                     /locus_tag="VF686_07970"
     CDS             complement(194..826)
                     /locus_tag="VF686_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661789.1"
                     /translation="MTVTYNPDPLNPYSDRPEVRPLTGERLDLNARPEVAEEPFVPVY
                     ARRGKAARRTSGKVPTWMILAPIGVIALGAVAALMLMDNGGETTAPLAEPAATAPVVD
                     ATPLTSAAPLAAASTPAPVTAAPAPAPVVREATPVRRATAPAPVRRATAPAAIRTTTA
                     PAARVVTPAPASPTTSTLNTARTSPAPVVTVTPAPEPTPPAPVIVVQPLD"
     gene            complement(977..1450)
                     /locus_tag="VF686_07975"
     CDS             complement(977..1450)
                     /locus_tag="VF686_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HEX8661790.1"
                     /translation="MRLLIAATACAFALAGAVTGAASAQDYNADPNYGDITLQPGFTP
                     DPHLVSLRAGGSVSATAAGGGCSGYITSAPDFRLNWNGKGSLNIKISVLSKADTTLVV
                     NGPNGEWYCDDDTGEDNNPLITLGSIAGRYEIWVGTYSSGDPQPAVLSISELNNF"
     gene            1710..3509
                     /locus_tag="VF686_07980"
     CDS             1710..3509
                     /locus_tag="VF686_07980"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX8661791.1"
                     /translation="MGLVANIKRNMRFAKGLRRLLNRIKPITLDSADLVCDDWEEAVD
                     KFPEHAAIEDDRRTLTYRELDAMANRFGHWAQSRRLKRGDTIALVMLNRAEYVAAWLG
                     FAKVGVATALINTNLTGHALAHCLSISNASQVVTDEDCWKRVEDARALSGRNLMLWVL
                     GLKDEDEADERRGLDKPVRSGSSVRPARSARSGLTNRDTAMFIYTSGTTGMPKAARIT
                     HARARTYMRAFAGATAATDKDVTFNVLPLYHSTGGLVGVGPALLNGGKLVLRKRFSAT
                     SFWPDVKATGATLFVYIGELCRYLVNCPEHPDERSHKLRLAFGNGLRPDVWSDFQKRF
                     AIPDILEFYGSTEGNVSVFNFDGKPGAIGRVPGFLKKQINFRLVRFDMETEEPVRGAD
                     GLCQLAKAGEVGEALGMIGDDIRHAFSGYADKAASEKKILRDVFVRGDRWFRTGDLMK
                     QDSEGYVYFIDRIGDTYRWKGENVSTAEVEQRLADAPGVKEVIAYGVPVPGHEGKAGM
                     VALVVEGRFAAREFADWVDQELPVYARPAFVRLIKSADTTGTFKYKKTDLVADGFDPE
                     KVEGVLYVRGGKSGYTKLSEAARQAILNGETRL"
     gene            3581..>4320
                     /gene="motA"
                     /locus_tag="VF686_07985"
     CDS             3581..>4320
                     /gene="motA"
                     /locus_tag="VF686_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX8661792.1"
                     /translation="MFQIIGIVLLFALVFGSYVISGGKFEVILHALPHEMMAIGGAGL
                     AAFLISNSTTTMKATLGGLGKAFAGPKWKKQDYKDLLSLLFQLTKTMKSKGVIALESH
                     IEKPAESTIFQKYPKVLKDHFATDFICDTLRMMTMNLEDPHQIEDAMEKQLEKHHHEA
                     LEAPHALTALADALPALGIVAAVLGVIKTMGSISEPPEILGGMIGGALVGTFLGVFLA
                     YGLVGPIASRLKAIVDEESAFYKIIQSVL"
BASE COUNT          741 a         1427 c         1466 g          686 t
ORIGIN      
        1 gccatgggcc ggccggtcgg cgtgcagttc gaccgtctgg gggccctgct ggtcgccgac
       61 gacgtgggca atgtcatctg gcgggtgtcg ccggccacgc cccccgcgcc ccccgcgcgc
      121 cccgcgcccg cggcctgaca gacaaaagcc ccggcggagc gatccgccgg ggctttcggt
      181 tctgcccgcc cgatcagtcg agcggttgga ccacgatgac cggggccggg ggcgtcggct
      241 ccggagccgg cgtgaccgtc acgacgggag cgggcgacgt ccgggcggtg ttcagggtcg
      301 aagtcgtggg cgacgccgga gccggggtca caacgcgggc cgccggagcg gtcgtggtgc
      361 gaatcgccgc gggcgctgtc gcccggcgga ctggcgccgg agcggtagcg cgacgaaccg
      421 gggtggcttc tcgcaccaca ggcgccggag ccggagcggc cgtcacggga gccggagtcg
      481 aggcggcggc caacggggcg gcggatgtca gcggcgtggc gtccaccacc ggggcggtcg
      541 cggccggctc ggccaggggc gcggtggtct caccgccatt gtccatcaac atcagggcgg
      601 cgacggcgcc gagggcgatc acgccgatgg gcgccaggat catccaggtc ggaaccttgc
      661 cggaggtgcg gcgcgccgcc ttgccgcgac gtgcgtagac gggaacgaag ggctcctcgg
      721 ccacttcggg gcgcgcgttc aggtccagcc gctcgccggt cagcggcctg acttcgggac
      781 ggtcggaata ggggttcagc ggatcggggt tataggtgac ggtcatgtct gttcctccaa
      841 taacgtgggg aaggaacaga cttgtcagac cacggttccg cgatcttgtt gaagccgcga
      901 agaaacaccg ttgtcgccac aattgcggcg acaacggtga agctattgaa ccggcgctct
      961 acttggcgcc agcgggtcag aagttgttga gttctgaaat cgacaggacg gccggctgcg
     1021 gatcgccgct cgaataggtg ccgacccaga tttcgtaccg accggcgatc gagccgaggg
     1081 tgatgagcgg gttgttgtcc tcgcccgtgt cgtcgtcgca ataccattcg ccgttcggac
     1141 cgttgaccac cagggtggtg tcggccttgg acaggaccga gatcttgatg ttgagcgacc
     1201 ccttgccgtt ccagttcagg cggaagtccg gcgcgctggt gatatagccc gagcagccgc
     1261 cgccggcggc cgtggcgctg acgctgccgc ccgcgcgcag gctgaccaga tgcggatcgg
     1321 gcgtgaagcc cggctgcagg gtgatgtcgc cgtagttggg atcggcgttg tagtcctgcg
     1381 ccgacgcagc cccggtgaca gccccggcca gcgcgaaggc gcaggccgtg gcggcgatca
     1441 gaagtcgcat ggtcaaattc cccccggggc cctccacggc cccacgccgc acacccccgt
     1501 tcgccgatta agcccaagct tggggcgatc ttcaagcagg cgtcgcaaaa cggcacggtg
     1561 acgctacgtc accgccgcat cggcgccacg cctgcgggca aacccgcgta cataccggcg
     1621 caaacgccgt tcagattccc ctgccggttg accgcggcgg ccggcttgtc catcaccaag
     1681 ggggcgacgg cgacggagtg gatgcaacga tgggtcttgt ggcgaacatc aagcgcaaca
     1741 tgcgtttcgc caaggggctg cggcgcctgc tcaatcgcat caagccgatc accctcgata
     1801 gcgccgacct ggtctgcgac gactgggaag aggccgtcga caaattcccc gaacacgccg
     1861 cgatcgagga cgaccgccgc accctgacct atcgcgagct ggacgccatg gcgaaccggt
     1921 ttggccactg ggcccagagc cggcggctga agcgcggcga caccatcgcc ttggtgatgc
     1981 tgaaccgggc cgaatacgtg gccgcctggc tgggcttcgc caaggtcggg gtcgccaccg
     2041 ccctgatcaa caccaatctg accggccacg ccctggccca ctgtctatcg atctccaacg
     2101 cctcgcaggt agtgaccgac gaggactgct ggaaacgggt cgaggacgcg cgcgccctgt
     2161 cggggcgcaa cctgatgctc tgggtgctgg gcctgaagga cgaggacgag gccgacgaac
     2221 ggcgcggcct cgacaagccg gtgcgcagcg gctcctcggt gcggccggcc cgctcggcgc
     2281 ggtcgggcct gaccaaccgc gacacggcga tgttcatcta cacctcgggc accacgggca
     2341 tgcccaaggc ggcgcggatc acccacgccc gggcccgcac ctatatgcgc gccttcgccg
     2401 gagccacggc ggcgacggac aaggacgtca ccttcaacgt tctgccgctg tatcattcga
     2461 ccggcggcct cgtcggggtc ggccccgccc tgctgaacgg cggcaagctg gtgctgcgca
     2521 agcggttctc ggcgaccagc ttctggcccg acgtgaaggc cacaggcgcg accctgttcg
     2581 tctacatcgg cgagctgtgc cgttatctgg tcaactgccc cgagcatccc gacgaacggt
     2641 cgcacaagct gaggctggcc ttcggcaacg gcctgcggcc cgacgtctgg tccgatttcc
     2701 agaagcggtt cgccattccg gatatcctgg aattctacgg ctcgaccgag ggcaatgtct
     2761 cggtgttcaa tttcgacggc aagcccgggg ccatcggccg tgttccgggc ttcctcaaga
     2821 agcagatcaa tttccgcctc gtccgcttcg acatggagac cgaggagccg gttcgcggcg
     2881 ccgacggcct gtgtcagctg gccaaggcgg gcgaggtcgg cgaggcgctg ggcatgatcg
     2941 gagacgacat ccgccacgcc ttctcgggct atgccgacaa ggcggcctcg gagaagaaga
     3001 tcctgcgcga cgtcttcgtg cgcggcgacc gctggttccg caccggcgac ctgatgaagc
     3061 aggacagcga aggctatgtc tatttcatcg accggatcgg cgacacctac cgctggaagg
     3121 gcgagaacgt ctcgaccgcc gaggtcgaac agcggctggc ggatgcgccc ggcgtcaagg
     3181 aagtcatcgc ctatggcgtg ccggtgcccg ggcacgaggg caaggcgggc atggtcgccc
     3241 tggtcgtcga gggccggttc gccgcccgcg aattcgccga ctgggtcgat caggaactgc
     3301 cggtctacgc ccgtccggcc ttcgtgcggc tgatcaagtc cgccgacacc accggcacct
     3361 tcaaatacaa gaagaccgat ctggtcgccg acggcttcga tccagaaaag gtcgagggcg
     3421 tcctgtatgt gcgcggcggc aagagcggct acaccaagct gagcgaggct gcacggcagg
     3481 cgatcctgaa cggcgaaaca cgactttagg gttagccgcc ccttaatcat taacggcgat
     3541 ggtcggcgtc tctcgcgtgc gcaggacctg acgcccttcc atgttccaga ttatcggcat
     3601 cgtactgctg ttcgccctcg tgttcggcag ctacgtcatc tcgggcggca agttcgaggt
     3661 gatcctgcac gccttgcctc acgagatgat ggccatcggc ggcgccggtc tggccgcctt
     3721 cctgatctcg aacagcacca ccacgatgaa ggcgacgctg ggtggtctcg gcaaggcgtt
     3781 cgccgggccg aaatggaaga agcaggacta caaggacctg ctgtcgctgc tgttccagct
     3841 gaccaagacg atgaaatcca agggggtcat cgcgctggaa agccacatcg agaagccggc
     3901 cgagagcacc atcttccaga aatatcccaa ggtgctgaag gaccatttcg ccaccgactt
     3961 catctgcgac accctgcgca tgatgaccat gaacctcgag gatccccacc agatcgagga
     4021 cgccatggaa aagcagctgg agaaacacca ccacgaggcg ctggaagcgc cccacgccct
     4081 gactgccctg gccgacgccc tgcccgcgct cggtatcgtc gcggccgtgc tgggggtcat
     4141 caagaccatg ggctcgatct ccgaaccgcc ggaaatcctc ggcggcatga tcggcggggc
     4201 ccttgtcggc accttcctgg gcgtgttcct ggcctatggc ctggtcggcc ccatcgcgtc
     4261 ccggctgaag gcgatcgtcg acgaggaatc ggccttctac aagatcatcc agtcggttct
//
LOCUS       DASVVN010000494         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_492325,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000494 DASVVN010000000
VERSION     DASVVN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492325"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VF686_07990"
     CDS             complement(<1..1220)
                     /locus_tag="VF686_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8661793.1"
                     /translation="MPTIEVLTERAEAVSPQTLGSAVFQRFESEPDTLVIPVVEAGKP
                     VGLIERNAFLLKIAAPFGHALYSGRPVLHLMDDEPAVVEAGVSIDAFCDILLKSGPGA
                     LMRGFIVTRQGRYFGVGTAVSLLQAVNDRQRRQNDELAEQTRVLGDARTQALASARAK
                     SQFLAIMSHELRTPMNGVLAVAELLRRQPLNAQAQAHVTTIVDSSETLLRILQDALDL
                     SRAEAGELDLHCEPTPLRALMDDVQSLWAPRASQDDVTLMVSYEGDTELAAIIDGVRV
                     KQLFNNLIGNALKFARNGIVEVGLKAAAAGDMVRLEARVRDDGPGVEPGRVDEIFDPF
                     VHGSGPDGAGLGLAICRQIVERMDGRIWAENNPGRGATFAFDLDVPRAQVTAAAPSNV
                     ESIGDGELLTNPHIL"
     gene            1413..2129
                     /gene="queC"
                     /locus_tag="VF686_07995"
     CDS             1413..2129
                     /gene="queC"
                     /locus_tag="VF686_07995"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX8661794.1"
                     /translation="MSEFGPASEKSALVLFSGGQDSATCLAWALERFARVETVGFDYG
                     QRHAVEMDARLRVREGMLTVLPQWADRLGPDHVVDLTGYGRIAESALTEDRSIEMDAR
                     GLPTTFVPGRNLVFLTCAAALADRRGLDVLVGGMCETDFSGYPDCRRDTIDAMATALS
                     LGLDKPIVIETPLMALTKAETWALADRIGGAPLVELIIEASHTCYRGDREHRQAWGYG
                     CGDCPACELRAAGHSEWVAA"
     gene            2126..2761
                     /gene="queE"
                     /locus_tag="VF686_08000"
     CDS             2126..2761
                     /gene="queE"
                     /locus_tag="VF686_08000"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX8661795.1"
                     /translation="MTYSAKEVFLTVQGEGGQAGRPAVFLRFAGCNLWNGLERDRATA
                     VCTFCDTDFVGVDGDGGGKFASADALADHVQSMWRGRAGDPRLVVCTGGEPLLQLDAP
                     LIEALHARGFEIAIETNGTLAAPDGIDWICVSPKADAPVEQTTGQELKLVFPQAKAMP
                     DRFEHLAFERFWLQPMDGPDQAANTAAAIDYCLTHPKWRLSVQTHKYIGVR"
     gene            2758..>2965
                     /locus_tag="VF686_08005"
     CDS             2758..>2965
                     /locus_tag="VF686_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX8661796.1"
                     /translation="MSVFEITKAATFDAAHHFPNEPDGSPYRRMHGHSFSVEATVRGE
                     ALDGHGWVADLGALDRALKAAAEAL"
BASE COUNT          466 a          944 c         1074 g          481 t
ORIGIN      
        1 aggatgtgcg ggttggtcag aagctcgccg tcgccgatgc tttcgacatt ggacggggcg
       61 gcggccgtga cctgggcgcg cggtacgtcg aggtcgaagg cgaaggtcgc gccgcggccg
      121 ggattgttct cggcccagat gcgcccgtcc atacgttcga cgatctgccg gcagatggcc
      181 agacccagac cggcgccgtc gggacccgag ccgtgaacga acggatcgaa gatctcgtcg
      241 acgcgacccg gttcgacgcc cggcccgtcg tcgcgcacgc gcgcctccag ccggaccatg
      301 tcgccggcgg ccgccgcctt gaggcccact tcgacgatgc cgttgcgggc gaatttcagc
      361 gcattgccga tcaggttgtt gaacaactgt ttgacgcgca cgccgtcgat gatggcggcc
      421 agttcggtgt cgccctcata gctcaccatc agggtgacgt cgtcctggga cgcgcgcggc
      481 gcccacagcg actggacgtc gtccatcagg gcccgcaggg gggtgggttc gcaatgcaga
      541 tccagttcgc cggcctcggc gcgcgacagg tccagtgcgt cctgcaggat gcgcagcagg
      601 gtctcggatg aatccacgat ggtggtgacg tgcgcctgcg cctgggcgtt caggggctgg
      661 cggcgcagca gttcggccac ggccaggacg ccgttcatgg gcgtgcgcag ctcatggctc
      721 atgatggcga ggaactggct cttggcgcgg gcggaggcga gagcctgggt gcgggcgtcg
      781 ccgagcacac gggtctgttc ggccagttcg tcgttctgcc gccgctgccg gtcgttgacg
      841 gcctgcagca gggaaactgc ggtgccgacg ccgaaatacc ggccttggcg ggtgacgata
      901 aagccccgca tcagcgcgcc gggaccgctc ttcagcagga tgtcgcagaa ggcgtctatg
      961 ctgacgccag cctccaccac cgccggctcg tcgtccatca ggtgcaggac ggggcgcccg
     1021 gaatagaggg cgtgaccaaa gggggctgcg atcttcagca ggaaggcgtt gcgctcgatc
     1081 aggccgaccg gcttgccggc ctcgaccacg ggaatgacga gggtgtcggg ttcgctctcg
     1141 aagcgttgga agacagcgct gcccaacgtc tgcggcgaaa ccgcctcggc gcgctcggtc
     1201 aagacttcga tggtcggcat atccggccat aactccactg cgacacagca cctttgctcc
     1261 gaaagccttt gcagaagggt taaggtcagt aaccgccgtt tttgggccgc ctttgctctg
     1321 tccccggggc tggtgtagaa ggcccgcccg ccatcgaggc ggcccatccc cttgtttatg
     1381 cgcgcggcca aacggccgac cgcaggtttg tcatgagcga attcggcccc gctagcgaaa
     1441 aatccgccct cgtcctgttc tcgggcgggc aggacagcgc cacctgcctg gcctgggcgc
     1501 tggagcggtt tgcacgcgtc gagaccgtgg ggttcgacta tggccaacgg cacgccgtcg
     1561 agatggacgc gcgcctgagg gtgcgcgagg gcatgctgac ggtcctgccg cagtgggccg
     1621 accgtctggg tcccgaccat gttgtcgacc tgacgggcta tggccggatc gcggaaagcg
     1681 cgctgactga ggaccggtcg atcgagatgg atgcgcgggg cctgcccacc accttcgtgc
     1741 cgggacggaa tctggtgttt ctgacctgcg cggccgcgct ggcggaccgg cggggactgg
     1801 acgtgctggt cggcggcatg tgcgagaccg atttctcggg ctatcccgac tgccgccggg
     1861 acacgatcga cgccatggcg acggcgctca gcctcgggct ggacaagccg atcgtgatcg
     1921 agactccgct gatggccctg accaaggcgg agacctgggc gctggctgac cggatcggcg
     1981 gggcgccgct ggtcgagctg atcatcgagg cgtcgcatac ctgctaccgg ggcgatcgcg
     2041 agcatcggca ggcctggggg tatggctgcg gcgactgccc ggcctgcgaa ctgcgggcgg
     2101 cggggcattc ggaatgggtg gccgcatgac ctattcggcc aaggaagtgt tcctgaccgt
     2161 gcagggcgag ggcggacagg ccggacggcc ggcggtcttc ctgcgcttcg caggctgcaa
     2221 tctgtggaac gggctcgagc gggaccgcgc gacggcggtc tgcaccttct gcgacacgga
     2281 tttcgtgggc gtggacggcg acggcggcgg caagttcgcg agtgcggacg cgctggccga
     2341 ccatgtgcag tcgatgtggc ggggcagggc gggggacccc cggctggtcg tctgcaccgg
     2401 cggcgagccc ctgctgcaac tggacgcccc cctgatcgag gccctgcatg cgcgcgggtt
     2461 cgagatcgcg atcgaaacca acggcaccct ggcggcgccc gacgggatcg actggatctg
     2521 cgtcagcccc aaggccgatg cgccggtgga gcagacaacc ggtcaggagc tgaagctggt
     2581 ctttccgcag gcgaaggcca tgccggaccg gttcgagcac ctggccttcg aacgattctg
     2641 gctgcagccg atggatggcc ccgatcaggc ggccaatacg gcggcggcca tcgactattg
     2701 cctgacccac ccgaaatggc ggctcagcgt ccagacccac aaatacatcg gcgtaagatg
     2761 agcgttttcg aaatcaccaa ggcggccacc ttcgacgcgg cccaccattt tcctaacgag
     2821 ccggacggta gtccgtaccg gcgcatgcac ggccactcgt tctccgtgga ggcgacggtg
     2881 cgcggcgagg cgttggacgg ccacggctgg gtcgcggacc tgggtgcgct ggaccgcgcg
     2941 ctgaaggcgg cggcggaagc actgg
//
LOCUS       DASVVN010000495         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_440152,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000495 DASVVN010000000
VERSION     DASVVN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440152"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VF686_08010"
     CDS             complement(<1..682)
                     /locus_tag="VF686_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8661797.1"
                     /translation="MDAAVPKRSDQELGGAPEGLDALTVAERLRAEGGVGVFVARDYA
                     RMGEFVQAFGFFAQDIEVIEFPAWDCLPYDRLSPSSSVSAERMAALTKLAQRQQGDSR
                     ATLVVTTVGAAIQRTPPREVTCGAGFETTVGLDLDTAALERYFAANGYMRASTVSERG
                     EFAVRGGVIDVFPPGFEEPVRLDMFGSELESIRTFDPATQRSTGQRREVSLAPVSEAL
                     LDPDSISRF"
     gene            complement(682..1002)
                     /locus_tag="VF686_08015"
     CDS             complement(682..1002)
                     /locus_tag="VF686_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX8661798.1"
                     /translation="MADTDARPEEASARERLGRIVFRAWRRGFREADLVLGPFADQVG
                     PTLDEDELNSLEALLAEDDDHRLYAWIIGSEPTPAAHETAVMSKIRDFMRDHVAAVVA
                     EGAG"
     gene            1086..>1812
                     /locus_tag="VF686_08020"
     CDS             1086..>1812
                     /locus_tag="VF686_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX8661799.1"
                     /translation="MRPEILFPLFAEASTLKGVGPKVLPLVQKLAGPLVRDVVFLSPS
                     GLVVRRPMTAVDAVEGQVGIFEISIDRLIVPSRPGAPVKVRATDATGFVHLIWFGGSA
                     QHIDRLLPRGQIRLVTGKVERFNNEVQIVHPDIFTPEQAGDIPAIEPVYPATQGLTSR
                     VIRKLVQASLQHAPDLPEWQDPAWLAKTRWPRWRAAVERLHAPEAEPDLEPDSPARQR
                     LAYDELFAHQLALARRRRARQITP"
BASE COUNT          271 a          693 c          571 g          277 t
ORIGIN      
        1 ggaaccgcga gatgctgtcc gggtccagca gggcctccga caccggcgcc agcgaaacct
       61 cgcgcctctg accggtcgac cgctgcgtcg ccggatcgaa ggtgcggatc gattccagct
      121 ccgagccgaa catgtccagc ctcaccggct cctcgaagcc gggcgggaag acgtcgatca
      181 ccccgccgcg caccgcgaac tcgccgcgct ccgacaccgt cgaggcgcgc atatagccgt
      241 tcgccgcgaa ataccgctcg agcgccgccg tatcgaggtc gaggccgacc gtggtctcga
      301 acccggcgcc gcaggtcacc tcgcgtggcg gggtccgctg gatcgccgcc ccgaccgtgg
      361 tcaccaccag cgtcgcgcgg ctgtcgcctt gctgccgctg ggccagtttc gtcagcgccg
      421 ccatccgctc cgccgacacc gaggacgacg ggctgagccg gtcgtagggc aggcagtccc
      481 aagccgggaa ttcgataacc tcgatatcct gggcgaagaa gccgaacgcc tgcacgaact
      541 cgcccatccg ggcgtagtca cgcgccacga acacgccgac gccgccctcg gccctcagcc
      601 gctcggcgac ggtcagcgcg tccagcccct cgggcgcccc gcccagttcc tgatcgcttc
      661 gtttgggaac cgctgcgtcc atcagcccgc gccctcggcc acgacagcgg cgacatggtc
      721 gcgcatgaag tcgcggatct tgctcatcac ggcggtctcg tgcgccgccg gcgtcggctc
      781 gctgccgatg atccaggcgt acagtcggtg atcgtcgtcc tcggccagca gagcctccag
      841 ggaattcagt tcgtcttcgt ccagcgttgg accgacctga tccgcgaacg ggcccagcac
      901 caggtcggcc tcgcggaacc cgcgccgcca ggcccggaac acgatccggc ccagccgctc
      961 acgggcggaa gcttcttcgg gacgcgcgtc ggtatcggcc acaagcttcc ttgcccggtc
     1021 ggtcagtaaa caggacgggt cagatagcgt tccctccgcc ttttcgccag ctatgctgtc
     1081 gcccgatgcg gcccgagatt ctctttcccc tgttcgctga ggcctccacc ctgaagggcg
     1141 tgggccccaa ggtgctgccg ctcgtacaga agctggcggg cccgctggtg cgcgacgtcg
     1201 tgttcctgtc gccgtcgggg ctggtcgtgc gccgtccgat gaccgccgtt gacgccgtcg
     1261 agggtcaggt cggaatcttc gagatcagca tcgaccgcct gatcgtgccg tccagacccg
     1321 gcgctccggt caaggtgcgg gcgacagacg ccaccggttt cgtccatctg atctggttcg
     1381 gcggctcggc ccagcatatc gaccgcctgc tgccgcgcgg ccagatccgg ctcgtcacgg
     1441 gaaaggtcga gcgtttcaac aacgaggtgc agatcgtcca ccccgacatc ttcacccccg
     1501 aacaggccgg cgacattccg gccatcgagc ccgtctatcc ggcgacccag ggcctgacct
     1561 cccgcgtcat ccgcaaactg gtccaggcct cgctccagca cgcgcccgac ctgcccgaat
     1621 ggcaggaccc cgcatggctg gcgaagaccc gctggccccg ctggcgcgcc gccgtcgaac
     1681 gcttgcacgc ccccgaggcc gagcccgatc tggagccgga ctccccggcg cgccagcggc
     1741 tggcctatga cgagctgttc gcccatcagc tggcgctggc ccgccgccgc cgcgcccgcc
     1801 agatcacgcc gg
//
LOCUS       DASVVN010000496         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_492368,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000496 DASVVN010000000
VERSION     DASVVN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492368"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1507)
                     /locus_tag="VF686_08025"
     CDS             complement(44..1507)
                     /locus_tag="VF686_08025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8661800.1"
                     /translation="MTDGAGGEAAPEAARTWGRWFGRPGHSLTRRLIWLASAWIIVAL
                     VLTGWALTTQYQESALRRLGNVLADTIDEVVVATDASGPDGLTVAEIKDARTLRALSG
                     KYWAVAEPTADGSLRVLVRSPSLAGESLTIPPDLPARLQGAFGATISYNDLDGLRPNG
                     EGPLGRPLRVAASIKQLPGRTRPLVFLAGLDRSNIDNDTRQFATFTWTALLILGAGLV
                     IAVFLQVQIGLRPLFALRNEIADVRMGRSGRIARSYPVEIQPLAEQVNRLLDHNQETV
                     ERQRTHVGNLAHALKTPLSVMLAEAGTQSGPLPDMVRKQTELMKAQVDHHLRRARAAA
                     RAQLLGERTPVAEVLDEMAVMLERVFESKGVEIDWRAPDELGFRGERQDLQEILGNLM
                     ENACKWARRRIRISAGPTGLGQMVVVVEDDGPGLPAEQREAALERGARMDETTPGSGL
                     GLSIVVELTRAYGGRITLADSDLGGLKVLLELPAAEA"
     gene            complement(1504..2184)
                     /locus_tag="VF686_08030"
     CDS             complement(1504..2184)
                     /locus_tag="VF686_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8661801.1"
                     /translation="MRVLLVEDDVDLSRQLKLALGDAGYAVDHAADGEEAHFLGDTEP
                     YDVVVLDLGLPKIDGVSVLERWRRDGKTTPVLILTARGAWSDKVSGFDAGADDYLTKP
                     FHTEELLARLRALLRRSAGIASATLSCGGLRLDPRAARATVNGEPLRLTSLEYRLLHY
                     LMMHQGRVISRTELVEHLYDQDFDRDSNTIEVFVGRLRKKIGSDRIETVRGLGYRLTP
                     LAGESEAA"
     gene            complement(2185..2472)
                     /locus_tag="VF686_08035"
     CDS             complement(2185..2472)
                     /locus_tag="VF686_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661802.1"
                     /translation="MNRFSAIGLAALIAAAPLSQAVAQDRGPRAERQQQEERPRVSMG
                     EAVQRASSGRPGRFLGASDRGGTIVVRWEYPGGRIADIMVDGRTGRVIGER"
BASE COUNT          395 a          874 c          875 g          384 t
ORIGIN      
        1 agtgcacggg cacggttaac aagcgctatt cgagggcgcg ggatcaggcc tcggcggccg
       61 gcaattccag cagcactttc agcccgccca gatcgctgtc ggccagggtg atgcgtccgc
      121 cataggcccg cgtcaactcg accacgatcg acaggcccag acccgaaccg ggcgtggtct
      181 cgtccatccg cgcgccacgt tccaaggcgg cctcgcgctg ttcggcgggc aggccggggc
      241 cgtcgtcctc gaccaccacc accatctgcc ccagacccgt cggaccggcc gaaatccgga
      301 ttcggcggcg ggcccatttg caggcgttct ccatcaggtt gccgaggatt tcctgcagat
      361 cctgccgctc gccgcggaat cccagctcgt ccggcgcgcg ccagtcgatt tcgacaccct
      421 tcgactcgaa aacccgctcc agcatgaccg ccatctcgtc gagaacctcg gcgaccggcg
      481 tgcgttcgcc cagcagctgg gcccgtgcgg cggcgcgggc gcggcgcagg tggtggtcga
      541 cctgggcctt catcagctcg gtctgtttgc gcaccatgtc aggcagcggg ccggactggg
      601 tccccgcctc ggccagcatg acggacagcg gcgtcttcag cgcatgggcc agattgccga
      661 cgtgggtccg ctggcgttcc acggtctcct gattgtggtc cagcaggcgg ttgacctgct
      721 cggccagagg ctggatctcg accggatagc tgcgggcgat gcggccgctg cggcccatgc
      781 gcacgtcggc gatctcgttg cgcagggcga acagggggcg caggccgatc tggacctgaa
      841 ggaagaccgc gatcaccaga ccggcgccga ggatcagcag ggccgtccac gtaaaggtcg
      901 cgaactggcg ggtgtcgttg tcgatatttg accggtccag tcccgcgagg aagacgagcg
      961 gacgggtccg gccgggcaac tgctttatgc tggcggcgac gcgcagcggc cggcccagcg
     1021 gcccctcgcc gttgggacgc aggccgtcga ggtcgttgta gctgatcgtc gcgccgaacg
     1081 cgccttgcag ccgtgcgggc agatcggggg ggatggtcag actttcgccc gccagcgacg
     1141 gagatcggac aagcacccgc agagagccgt cggccgtcgg ttcggccacc gcccagtatt
     1201 tgcccgacag ggcgcgcagc gtgcgggcgt ccttgatctc ggccacggtg aggccatcgg
     1261 gcccgctcgc gtcggtcgcg acgaccactt catcgatggt gtcggccagc acattgccca
     1321 gtcggcgcaa ggcgctttcc tgatactggg tggtcagggc ccagccggtc aggaccaggg
     1381 cgacgatgat ccaggccgac gccagccaga tcagccgccg cgtcagactg tgccccgggc
     1441 gaccgaacca gcggccccag gttcgggcgg cttccggagc cgcttcgccc cccgccccat
     1501 cggtcacgcc gcttcggact ccccggccag cggcgtcagg cgatagccga ggccgcgcac
     1561 ggtctcgatg cgatcggagc cgatcttctt gcgcagccgt ccgacgaaca cctcgatggt
     1621 gttggagtcg cggtcgaagt cctgatcgta caggtgctcg accagctcgg tgcggctgat
     1681 gacccggccc tggtgcatca tcagatagtg cagcagccgg tattccagcg aggtcagacg
     1741 aaggggctcg ccattgacgg tggcccgggc ggcgcgggga tccagccgaa gtccgccgca
     1801 cgacagcgtc gcagaagcga tgccggccga acgccgcagc agggcccgca gacgggccag
     1861 cagctcctcg gtgtggaagg gcttggtcag atagtcgtcg gcgcccgcgt caaagcccga
     1921 caccttgtcc gaccaggcgc cgcgtgcggt caggatcagc accggcgtgg tcttgccgtc
     1981 gcggcgccaa cgctccagca ccgagacgcc gtcgatcttg ggcaggccga ggtccagcac
     2041 caccacgtca tagggttcgg tgtcgccgag gaaatgcgct tcctcgccgt ccgcggcgtg
     2101 atccacggca tagccggcgt cgcccagggc cagtttcagc tggcgcgaca gatcgacgtc
     2161 gtcttcgaca agcaggaccc gcattcagcg ttctccgatg acgcggccgg tgcggccgtc
     2221 aaccatgatg tcggcgatcc ggccgccggg gtattcccag cgaaccacaa tggtcccgcc
     2281 gcggtcgctg gcgcccagga agcggccggg acggcccgac gaggcccgct gcacggcctc
     2341 gcccatgctg acacgcgggc gttcttcctg ctgctggcgc tcggcgcgcg gaccacggtc
     2401 ctgcgcgacc gcctgcgaca ggggcgcggc ggcgatcagg gcggcgagtc ctatggcgga
     2461 aaagcggttc atgggccgag acttagactc cagcctctga acgcgggctg aacgggcagc
     2521 atagacat
//
LOCUS       DASVVN010000497         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_44871, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000497 DASVVN010000000
VERSION     DASVVN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44871"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="lptE"
                     /locus_tag="VF686_08040"
     CDS             <1..357
                     /gene="lptE"
                     /locus_tag="VF686_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX8661803.1"
                     /translation="DRLGYRVREQLEDALGRDGSQTPLWRLQTDLDQSRRSLGRRIDD
                     TATRYELTVRGAWTLTPAGGGEPIAGVESVTTTYAAADQPYAAIAAQQDGEERAAAEL
                     ARLIRLDIMRALSAQP"
     gene            368..1420
                     /gene="holA"
                     /locus_tag="VF686_08045"
     CDS             368..1420
                     /gene="holA"
                     /locus_tag="VF686_08045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX8661804.1"
                     /translation="MILTKRPEVDRFLSSPDRGVRAAVIHGKDRSGVGERAEILCKAI
                     TPDLNDPFNVTVLTDSDIDGDEARLEEALTALSMIGGRRLVRVRLGSEKASIDRMLAA
                     AVKHHAEGAYNPDAMLVIEAGALGRDSALRKAAEGAKEAVGIACYEDETGDVARMTRE
                     ALAADKVGLTGDALDRFVSRLPRERGLMRQEIERLALYIGPGSGKTIDVAELEQHLGV
                     EADASLQDAALQAFGGRPAPAQSGLRRAFAEGESPVMAVRQASIHLGRLRRINVLQAN
                     GSGAKEAAKAAGVFWKQEAEMLRQARSWRLEDLDRVLDSVNTADIATKTTGSPDQLIA
                     ERLLLEISARARRLGL"
     gene            complement(1520..1705)
                     /locus_tag="VF686_08050"
     CDS             complement(1520..1705)
                     /locus_tag="VF686_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661805.1"
                     /translation="MTDKPSNPTAEAMKRALAAKKAGGGATPDGLKGGGRAEQKAQQV
                     RNLALAKPAFRKASKRG"
     gene            complement(1729..2619)
                     /locus_tag="VF686_08055"
     CDS             complement(1729..2619)
                     /locus_tag="VF686_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8661806.1"
                     /translation="MSERQRGLGRGLSALLGEQVAESAPVDGAPAPAGVRMAPIESLK
                     PNPEQPRKIFNNDDLAELSASIRDKGVLQPILVRSQPGADGVWQIIAGERRWRAAQLA
                     RLTEVPVVVREMDDVEVFEVAIIENVQRADLNPLEEADAYRMLMERFGRTQDAVAGVV
                     GKSRSHVANTLRLLQLPEEVLWFVRNDKMSAGHARALITAPNPAALAQKIVDEGLNVR
                     QAEALARRAAEGPKPGKARTTLSASGEGAADVAALEQDLADALGLKVQLADKGGKGEI
                     TIRYGTLEQLDDLCRRLMRG"
     gene            complement(2648..3475)
                     /locus_tag="VF686_08060"
     CDS             complement(2648..3475)
                     /locus_tag="VF686_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8661807.1"
                     /translation="MSPTGKTPQGRTRVLAVSNQKGGVGKTTTAINLGTALAAIGEKV
                     LIVDMDPQGNASTGLGVPRETRRVTIYDVIVDGRSVDDSAVQTSVPGLHIIPADADMS
                     GVEIELSQADRRSFRLRDALAGQGAEGQTRYDYVLIDCPPSLNLLTLNAMAAADAVLV
                     PLQCEFFALEGLTQLMKTIEMVRQSLNPTLEIQGLVLTMYDRRSALSGQVAADVRSHF
                     GEKVYEAVIPRNVRVAEAPSFGKPALIYDLKCAGSQAYLRLAREVVAREKSRRLQAA"
     gene            complement(3459..4079)
                     /gene="rsmG"
                     /locus_tag="VF686_08065"
     CDS             complement(3459..4079)
                     /gene="rsmG"
                     /locus_tag="VF686_08065"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8661808.1"
                     /translation="MTPSEFQTLLNATPAQMADLETFIARLTEANAVMNLVGPDSLPD
                     VWNRHIRDSAQLLEMKPEAQTWADLGAGAGFPGLVLAILLKDRADSHVWLIDSLGKRC
                     RFLQEMVGALSLRATVINGRAEEQRVKVDVVTARALAPMERLLGYAQPYLQRGAQGLF
                     LKGEKAEAELIEARKVWQFDSDLSVSRSDPRGRIVSVRSLRRVPNR"
BASE COUNT          674 a         1454 c         1482 g          679 t
ORIGIN      
        1 gaccgcctcg gctaccgggt gcgggaacag cttgaggacg cccttggccg tgacggctcg
       61 cagacccctc tgtggcggtt gcagaccgat ctcgatcagt cgcgccggtc gctgggccgc
      121 cggatcgacg acaccgccac ccgatatgaa ctgaccgtgc gcggggcctg gaccctgacc
      181 ccggccggcg gcggcgagcc gatcgcgggc gtggaaagcg tcaccaccac ctatgccgcc
      241 gccgaccagc cctacgcggc tatcgccgcc cagcaggacg gcgaggaacg cgccgctgcc
      301 gaactggcgc gcctgatccg gctggacatc atgcgggcgc tgtcggcgca gccttgaggg
      361 tcgcccgatg atcctgacca aacggcccga ggtcgaccgc ttcctgtcat cgcccgaccg
      421 cggcgtccgc gccgccgtca tccacggcaa ggaccggtcc ggcgtgggcg agcgcgcgga
      481 aatcctgtgc aaggcgatca cgcccgacct gaacgacccc ttcaacgtca ccgtcctgac
      541 cgacagcgat atcgatggcg acgaggcgcg gctggaagag gccctgactg cgctcagcat
      601 gatcggcggc cgccgtctgg tccgcgtgcg gctgggctcc gagaaggcct cgatcgacag
      661 gatgctggcc gccgcggtca agcaccacgc cgagggcgcc tacaaccccg acgccatgct
      721 ggtcatcgag gccggcgccc tcggacggga ctccgcgctc cggaaggccg ctgaaggagc
      781 caaagaggcc gtcggcatcg cctgctatga ggatgagacg ggcgatgtcg cgcggatgac
      841 gcgcgaggcc ttggccgcgg acaaggtcgg gctgacgggg gacgccctgg accggttcgt
      901 cagccggctg ccgcgcgaac gcgggctgat gcggcaggag atcgagcggc tggccctcta
      961 tatcgggccc ggctcgggca agaccatcga cgtcgccgaa ctggaacagc atctgggcgt
     1021 cgaggccgac gcctcgttgc aggacgccgc gctgcaggct ttcggcggcc gccccgcccc
     1081 ggcccagtcc ggcctgcgcc gcgccttcgc cgagggagaa tcgcctgtca tggcggtgag
     1141 acaggcttcg atccatctgg gccgactgcg ccgcataaac gtgctccagg ccaacggctc
     1201 gggcgccaag gaggcggcca aggcggcggg ggttttctgg aaacaggagg ccgaaatgct
     1261 gcgtcaggcg cgcagctggc ggctggagga cctcgaccgg gtgctggaca gcgtcaacac
     1321 cgccgacatc gcgaccaaga cgaccggctc gcctgaccag ctgatcgccg aacggctgct
     1381 gctggagatt tctgcccggg cgaggcggct gggactctag gttcgcatcg cagacggggg
     1441 caaaccttca tcaagtttca ggcccgcgct gccgaaggcg tcggaatagt ctcgaaagac
     1501 taatcgaaat caccacgaac tagccccgct tggacgcctt gcggaaggca ggcttggcca
     1561 gcgcgagatt gcggacctgc tgggcctttt gctcggcgcg gccgccgcct ttcagaccgt
     1621 cgggcgtggc gccgccgccc gccttcttgg ccgcgagggc gcgcttcatg gcttcggcgg
     1681 tggggttgct cggcttgtca gtcattggag cagcctagca ggttgagatc agccccgcat
     1741 caggcggcgg cacaggtcgt ccagctgttc cagcgtgccg tagcggatgg tgatctcgcc
     1801 cttgccgccc ttgtcggcca gctggacctt cagacccaag gcgtcggcca gatcctgctc
     1861 aagcgccgcg acgtcggccg cgccctcgcc cgaggcggac aaggtcgtcc gcgccttgcc
     1921 gggcttcggg ccttcggcgg cgcgacgcgc cagcgcctcg gcctgacgga cgttcagccc
     1981 ctcgtcgacg atcttctggg caagggcggc cgggttgggg gccgtgatca gcgcgcgggc
     2041 gtggccggcg ctcatcttgt cgttcctgac gaaccacagg acctcttcag gcagctgcag
     2101 caggcgcagg gtgttggcga catggctgcg gctcttcccg acgacgccgg cgacggcgtc
     2161 ctgggtgcgg ccgaaccgct ccatcagcat gcggtaggcg tcggcctctt ccagcggatt
     2221 gaggtcggcg cgctggacgt tctcgataat cgcgacctcg aaaacctcga cgtcgtccat
     2281 ctcgcggacc acgaccggca cctcggtgag ccgggccagc tgggcggcgc gccagcggcg
     2341 ttcgccggcg atgatctgcc agacgccgtc cgcgcccggc tgcgaccgca ccaggatcgg
     2401 ctgcagcacg cccttgtcgc ggatcgaggc cgacagttcg gccagatcgt cgttgttgaa
     2461 gatcttgcgc ggctgttccg gattcggctt caggctctcg atcggggcca tgcgcacgcc
     2521 ggcgggcgcg ggcgcgccgt cgaccggggc gctctcggcc acctgttctc ccagaagggc
     2581 ggaaaggccc cggccgaggc cgcgctgacg ttcgctcaag acactgattc cgttctgcta
     2641 ttcttcatca cgcggcctga agccgacgcg atttctcgcg cgccactacc tcgcgggcga
     2701 ggcgcagata ggcctgactg ccagcgcatt tcaggtcgta gatcagggcc ggcttgccga
     2761 atgacggcgc ctccgccacc cgcacgttgc gggggatgac ggcctcatag accttctccc
     2821 cgaagtgcga acgaacgtcg gcggcgacct gtccggacag ggcgctgcgc cggtcgtaca
     2881 tggtcaggac cagtccctgg atttccagcg tagggttcag gctctgccgc accatttcga
     2941 tggtcttcat cagctgggtc aggccctcga gggcgaagaa ctcgcactgc agcggcacca
     3001 gcacggcgtc ggccgcggcc atggcgttga gggtcagcag gttcagcgac ggcgggcagt
     3061 cgatcagcac atagtcgtac cgggtctgac cctccgcccc ctgccccgcc agcgcgtcgc
     3121 gcaggcggaa cgagcggcgg tcagcctggc tcagttcgat ctcgacgccc gacatgtcgg
     3181 cgtcggccgg aatgatgtgc aggccgggca ctgaggtctg gacggcggaa tcgtcgaccg
     3241 aacggccgtc gacgatgacg tcatagatgg tcacccgccg tgtttcacgt ggaacaccca
     3301 gacccgtgga ggcattgccc tgcggatcca tgtcgacgat gagcaccttc tcgccgatcg
     3361 cagccagcgc ggtgccgaga ttgatcgccg tcgtagtctt gcccacgccg cctttctggt
     3421 tcgagacagc gaggactcgg gtacgccctt ggggagtctt accggttggg gacacgtcga
     3481 aggctccgga cggaaacgat acggccgcgc ggatcactgc gcgagacgga gaggtcgcta
     3541 tcgaactgcc agaccttgcg ggcttcgatc aactcagcct cggccttttc ccccttgaga
     3601 aacagcccct gtgcgccgcg ctgtagataa ggctgtgcat agccgagaag tcgctccatc
     3661 ggagccagcg cccgggcggt gacgacgtcg accttgaccc tctgctcctc ggcccggcca
     3721 ttgatcaccg tggcgcgcag ggacagggcg ccgaccattt cctgcaggaa gcggcagcgt
     3781 ttgccgagac tgtcgatcag ccagacgtgg ctgtcggcgc gatccttgag caggatggca
     3841 agcacgaggc cggggaagcc agccccggcg ccgaggtcgg cccaggtctg cgcctcgggc
     3901 ttcatctcca gcagctgggc ggaatcccgg atgtggcggt tccacacgtc gggcaggctg
     3961 tcggggccga cgagattcat caccgcattg gcctcagtga ggcgggcgat gaaggtttcg
     4021 aggtcggcca tttgcgccgg ggtggcgttc aagagcgtct ggaattcgga cggcgtcatc
     4081 cctcgtctcc tccccatgca atggggaggg ggaccgcaaa gcggtggagg ggcgcccctc
     4141 cgtcacggcg cggtgcggcg cgccacctcc ccagttcatg gggaggagac agccggtcca
     4201 tcatgccgcc acccgcgtcc gtttgacgtg ggagagaagc gccgtaagcg cgcccggggt
     4261 cataccctcg atgcgtccgg cctgaccca
//
LOCUS       DASVVN010000498         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_333895,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000498 DASVVN010000000
VERSION     DASVVN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333895"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /gene="rimM"
                     /locus_tag="VF686_08070"
     CDS             <1..420
                     /gene="rimM"
                     /locus_tag="VF686_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506629.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX8661809.1"
                     /translation="KYGVLLRADGTPVLTLTSARPEKTAVVGRAKEIATKEQADAMRG
                     LKLYIHRDRLPEPDEDEFYLADLVGLEARDPEGVVLGSVRAVQNFGADDMLEIAPATG
                     GPTWYLPFTKEAVPELHIADGWLLAVRPAEIGEREPD"
     gene            443..652
                     /locus_tag="VF686_08075"
     CDS             443..652
                     /locus_tag="VF686_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX8661810.1"
                     /translation="MPSSVIRRFSYDEPDRRLRVTFTSGDVYDYFDVPPDLPADWKAA
                     FSKGQFFAERVRDRFAYELVEKAWR"
     gene            complement(657..1454)
                     /locus_tag="VF686_08080"
     CDS             complement(657..1454)
                     /locus_tag="VF686_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX8661811.1"
                     /translation="MPTGDLMKGKKGLIMGVANSNSIAWGIASQLAAQGAELAFTYLG
                     EGLERRVRPLAESVGAKLLIQADVTDDASMDAAFAELEKAFGTIDFVVHSVAFANKDE
                     LKGSFVENTSRDSFLLAMNISAFSFVDVAKRASRLMPNGGSLVTMTYLGSERAIPNYN
                     TMGVAKAALEAATRYIARDLGPKGIRCNAISAGAMRTLSLAGISGGRGMISQGRAMSA
                     MKEDTSMEGVAGAALWLCSDLGFSTTGEVIHVDAGFHMMGMAADEEG"
     gene            1652..2167
                     /locus_tag="VF686_08085"
     CDS             1652..2167
                     /locus_tag="VF686_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661812.1"
                     /translation="MIRSLAVLSVAVTLTGGSAALAQAGPPPLRVKVLLASDPHLDAA
                     ARASHDALLQSLSAGGPSGGWFQLLPTELDVAEFEHCTISAQDTRTCASRQLAAQEAT
                     GATVVVLAGGEGGHASWICVGTGARPMSPDRQAIRFDLNAGLDSASPLRMETRAAAAG
                     CITGAGAESGW"
     gene            complement(2174..3403)
                     /gene="fabB"
                     /locus_tag="VF686_08090"
     CDS             complement(2174..3403)
                     /gene="fabB"
                     /locus_tag="VF686_08090"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX8661813.1"
                     /translation="MRRVVVTGLGIVSSIGTGQDEVTASLRDAKSGVVAAPDHIQHGF
                     RSQVWAPPSLGVTAEDWAPHVDRRAARFLANGTAWGHIAFEEALKDSGMTADEIKSDR
                     IGVIVGEGGPSTQIILQAAATTVEKGSPKRIGPFAVPKAMASGPSAVLATWFELRGVN
                     YSISSACATSAHCIGAAAEQIAWGKQDVVFAGGVEDIDWSMSNMFDAMGAMSSNFNET
                     PAKASRAYDKDRDGFVIAGGAGIVVLEEYERAKARGATIYAEVTGYGANSDGYDMVAP
                     SGEGAERCMKIALEMAGNPRIDYLNPHGTSTPVGDSKEMGAVRNVFGDDLPMISSTKS
                     LTGHSLGAAGAQEAIYCLLMMKNGFAAESAHIENLDPEFEGMPILRKRHDGELKHVMS
                     NSFGFGGTNGTLILSKV"
     gene            complement(3462..3992)
                     /gene="fabA"
                     /locus_tag="VF686_08095"
     CDS             complement(3462..3992)
                     /gene="fabA"
                     /locus_tag="VF686_08095"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX8661814.1"
                     /translation="MSQSQHPSSFDYEALLAAGRGDLFGPGNAKLPAPPMLMFDRITQ
                     INGDGGAHGKGYVEAELDIHPDLWFFQCHFIGDPVMPGCLGLDAMWQLVGFYLGWIGG
                     PGRGRALGVGEVKFTGQVTPDIKKVVYKIDLKRVINRRLVMGIADGVLEADGVPIYTA
                     TDMRVGLFGATEPVGG"
     gene            complement(4075..4770)
                     /locus_tag="VF686_08100"
     CDS             complement(4075..4770)
                     /locus_tag="VF686_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8661815.1"
                     /translation="MPISNLLIKGFQPDDRDALVSLCSPVQFSLGDTLCAPGDHLSHL
                     LFVRSGIISIVTEMSDGRSVEAYMVGFEGMTGASATVVPASSHSRHVAQAAGSALRIE
                     AGAYRALAGSRASVRAIVGGYSAGLHTELEQSTACNALHHAEQRLAKWLLRCHDRVAG
                     DIILLTQEYLATMLGAQRTTVNEGAQTLQAVGAISCSRGKLTILDRTALEHAACECYR
                     ATGVGQEPDISED"
     gene            complement(4884..5579)
                     /locus_tag="VF686_08105"
     CDS             complement(4884..5579)
                     /locus_tag="VF686_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8661816.1"
                     /translation="MSDLPPGNRLIAAMNPEDRDALLAQCVRTEFPQGFVFYEPGDAI
                     DHMHFMETGICSAVAVLEDGRTVETIMVGPEGVTGVVASIVPHPSLTRNVAQVGGASQ
                     RIEAGRLRSLTATRPGIRDVIAGFMSSMQGELEQSTACNALHRAEQRFAKWLLRCHDR
                     VEGEPLNLTQEYLASMLGSQRTTVNEAAQTLQKAGAIKYSRGRITVLDRAALERVACE
                     CYRATVSVRDRRE"
BASE COUNT          878 a         1877 c         1953 g          933 t
ORIGIN      
        1 aagtacggcg tcctgctgcg cgctgacggc acgcccgtcc tgaccctgac ctcggcccga
       61 cccgagaaga ccgctgtggt cggtcgggcg aaggagatcg ccaccaagga acaggccgac
      121 gcgatgcgcg ggctgaagct ctacatccac cgcgaccgcc tgcccgagcc ggacgaggac
      181 gaattctacc tcgcagatct ggtcggtctg gaggcccgcg accccgaggg cgtcgtgctg
      241 ggctcggtcc gcgcggtgca gaatttcggc gccgacgaca tgctggagat cgccccggcg
      301 acgggcggcc cgacctggta tctgccgttt accaaagagg ccgtgcccga gctgcacatc
      361 gccgacggct ggctgctggc cgtccgaccc gccgagatcg gcgagcgcga accggactag
      421 gcggctccgc gttccggagg gcatgccctc cagcgtcatc cgccgcttca gctatgacga
      481 accggaccgc cgcctgcgcg tgaccttcac ctcgggcgac gtctacgact atttcgacgt
      541 gccgccggac ctgcccgcgg actggaaggc ggctttctcg aagggtcagt tcttcgccga
      601 gcgggtcagg gaccggttcg cctatgagct ggtcgagaag gcctggcgct gacggatcag
      661 ccttcctcgt ccgccgccat gcccatcatg tggaagccgg cgtcgacgtg gatgacctcg
      721 cccgtggtcg agaagccgag gtcggagcac agccacaggg cggcgccggc gacgccctcc
      781 atcgaggtgt cctccttcat cgccgacatg gcgcggccct ggctgatcat gccacggccg
      841 cccgagatgc cggccagcga cagggtgcgc atggccccgg ccgagatggc gttgcagcgg
      901 atgcccttgg gccccagatc gcgggcgatg tagcgggtcg ccgcctccag cgccgccttg
      961 gccacgccca tggtgttgta attggggatg gcgcgctcgg agccgagata ggtcatggtc
     1021 accagcgagc cgccgttggg catcaggcgc gaggcgcgct tggccacgtc gacgaagctg
     1081 aaggccgaga tgttcatggc cagcaggaag ctgtcgcggc tggtgttctc gacgaacgac
     1141 cccttcagct cgtccttgtt ggcgaaggcc accgagtgga cgacgaagtc gatcgtgccg
     1201 aaagcctttt ccagctcggc gaaggcggcg tccatggagg cgtcgtcggt gacgtcggcc
     1261 tggatcagca gtttggcgcc gacgctctcg gccagcgggc ggacgcggcg ctcgagcccc
     1321 tcgccgaggt aggtgaaggc cagttcggcg ccctgggccg ccagctggga ggcgatgccc
     1381 caggcgatgg agttgctgtt cgccacaccc atgatcaggc ccttcttgcc cttcatcagg
     1441 tcgcccgtgg gcatgttcca gccgctctcg ctcgccatgg tccgtctccg tgttcttgtt
     1501 ggcctgacgc tcgcgaagcg gtcgctcgcc gcttgaggcc gtcgcggagg tttagcagcg
     1561 accggccagc ggtgaaagac catccgtcag gggcgcttgg ccgggcggaa ctgacgggct
     1621 agcgtgacgg tccgcgtcag ggagcccgcc gatgatccga tcactcgccg ttctgagcgt
     1681 ggccgtaacc cttaccggag gctccgcggc cctcgcccag gccggtccgc cgccgttgcg
     1741 ggtcaaggtc cttctggcct ccgaccctca tctggacgca gcggcgcggg ccagtcacga
     1801 cgccctgctg cagagcctgt ccgcgggcgg gccatccggc ggctggttcc aactgctacc
     1861 caccgaactg gacgtggccg agttcgagca ctgcactatc tcggcgcagg acacccgcac
     1921 ctgcgccagc cgccagttgg ccgcgcaaga ggcgacgggc gcgacggtcg tcgtcctcgc
     1981 cggcggcgag ggcggtcacg cgagctggat ctgtgtcggg acaggcgcgc gcccgatgtc
     2041 gccggatcgg caggccattc gcttcgacct gaacgccggc ctcgatagcg cgtcaccctt
     2101 gcgcatggag acccgcgcgg cggcggccgg ctgcattacc ggcgccggcg cggagagcgg
     2161 ctggtagcgc cggtcagacc ttgctgagga tcagcgtccc gttggtcccg ccgaagccga
     2221 agctgttgga catgacgtgc ttcagttcgc cgtcgtggcg tttgcgcagg atcggcatgc
     2281 cctcgaactc ggggtcgagg ttttcgatat gggcgctctc ggcggcgaag ccgttcttca
     2341 tcatcagcag gcagtagatc gcctcctgcg ctccggcggc gcccagcgaa tggcccgtca
     2401 gcgacttggt cgagctgatc atcggcaggt cgtcgccgaa gacgttgcgg accgcgccca
     2461 tctctttcga gtccccgacc ggggtcgagg tgccgtgcgg gttcagatag tcgatccgcg
     2521 gattgccggc catttcaagc gcgatcttca tgcagcgctc ggcgccctcg cccgaggggg
     2581 cgaccatgtc gtagccgtcg gagttggcgc cgtagccggt gacctcggcg tagatggtcg
     2641 cgccacgcgc ctttgcccgt tcgtattcct ccagcaccac gatgcccgcg ccgccggcga
     2701 tgacgaagcc gtcgcggtcc ttgtcatagg cgcgcgaagc ttttgccggc gtttcgttga
     2761 agttggacga catggctccc atggcgtcga acatgttgga catggaccag tcgatgtcct
     2821 ccaccccgcc ggcaaagacc acgtcctgct tgccccaggc gatctgttcg gcggcggcgc
     2881 cgatgcaatg ggcgctggtg gcgcaggccg agctgatcga atagttgacg ccgcgcagct
     2941 cgaaccaggt ggccagcacg gccgaggggc ccgaggccat ggccttgggc acggcgaacg
     3001 gcccgatacg cttgggcgaa cccttttcga cggtggtggc cgccgcctgc aggatgatct
     3061 gggtcgaggg gccgccctcg ccgacgatga cgccgatgcg gtcggacttg atctcgtccg
     3121 cggtcatgcc gctgtccttc agcgcctcct cgaaggcgat gtggccccag gcggtgccgt
     3181 tggcgaggaa acgggcggcg cggcggtcga cgtgcggggc ccagtcctcg gccgtcacac
     3241 ccagagacgg cggggcccag acctgcgaac ggaacccatg ctggatatga tccggcgcgg
     3301 cgaccacgcc ggacttcgcg tcgcgcaggg acgcggtgac ctcatcctgc cccgtgccga
     3361 tggaggagac gatacccagg ccggtgacga caacacgccg catgatgtgg tttccttctt
     3421 gaactcaatt cgccgcgttg gccgcggtct tgaaacgacg gtcagccgcc gacgggttcc
     3481 gtcgcgccga acaggccgac ccgcatgtcg gtcgccgtat agatggggac gccgtcggct
     3541 tcgagaaccc cgtcggcgat gcccatgacc agccggcggt tgatcacccg cttcaggtcg
     3601 atcttgtaga caaccttctt gatgtccggc gtcacctggc cggtgaattt gacctcgccg
     3661 acgcccagcg cccggccgcg gcccggtccg ccgatccatc cgagatagaa gccgaccagc
     3721 tgccacatgg cgtccaggcc cagacagccg ggcatgacgg ggtcgccgat gaaatggcac
     3781 tggaagaacc agaggtcggg atggatatcc agctcggcct cgacatagcc cttaccgtgc
     3841 gcgccgccgt cgccgttgat ctgcgtgatc cggtcgaaca tcagcatcgg cggggccggc
     3901 agcttggcgt tgcccggacc gaacaggtcg ccgcggcccg cggccagcag ggcctcgtag
     3961 tcgaacgagg acggatgttg tgactggctc aacgcggcgc tccggcaggt gttgccgtgg
     4021 aggtacacga cgggaaccgg acgctcaatg ttttagagga agccgggccg tggctcagtc
     4081 ttcgctgata tcgggttcct gccccacccc ggtggcgcgg tagcattcgc acgcggcgtg
     4141 ttcgagcgcc gtgcggtcga ggatcgtcag cttgccacgc gaacagctga tggccccgac
     4201 cgcctgaagg gtctgggctc cttcattcac cgtggtgcgc tgggcgccca gcatggtggc
     4261 cagatactcc tgggtcagca ggatgatgtc tcccgcgacc cggtcgtggc agcgcagcag
     4321 ccatttcgcc agccgttgct cggcgtggtg cagggcgttg caggcggtgg actgctcaag
     4381 ctcggtgtgc aggccggccg aatagccgcc gacgatggcg cgaaccgagg cgcggctacc
     4441 ggccagcgcc cgataggcgc cggcttcgat gcgcagggcc gagccggcgg cctgcgccac
     4501 gtgccgggaa tggctcgagg ccggcaccac ggtggcggac gcgccggtca tgccctcgaa
     4561 accgaccata taggcctcga ccgagcgtcc gtcggacatc tcggtcacga tggagatgat
     4621 gccggagcgg acgaagagga ggtgactgag gtgatcgccc ggcgcgcaca gggtgtcgcc
     4681 caggctgaac tggaccgggc tgcacagcga gacgagagcg tcgcggtcgt ccggctggaa
     4741 tcctttgatc agaaggttcg agatcggcac gacacggctc cttcccggga agcgccgggg
     4801 tggtccgccg ccggggagcg gcgaagtcgc agcgtagccg ggccctatta tgcgccccga
     4861 accgtcggac tcctagtcag gcgttactca cggcggtccc ggaccgaaac agtggcgcgg
     4921 tagcattcgc aggccacccg ctcgagagcg gcgcggtcca gaaccgtgat acggccccgg
     4981 ctgtatttta tggcgccggc cttttgcaga gtctgtgcgg cctcgttcac cgtcgtccgt
     5041 tgcgagccca gcatgctggc cagatattcc tgcgtcaggt tcaggggctc gccctcgacc
     5101 cggtcgtggc agcgcagcag ccatttggcg aaccgctgct cggcccggtg cagggcgttg
     5161 cacgccgtcg actgttccag ctcgccctgc atggaggaca tgaagccggc gatgacgtcg
     5221 cgaatgccgg ggcgggtcgc ggtcagggac cgcaaacggc ccgcctcgat ccgctgtgaa
     5281 gcgccgccga cctgggcgac gtttcgggtc agggaggggt ggggcacgat ggaggcgacg
     5341 acgccggtta cgccctcggg accgaccatg atggtttcga cggtccggcc atcctccaga
     5401 acggccacgg ccgaacagat gcccgtctcc atgaaatgca tgtgatcgat ggcgtcgccg
     5461 ggctcgtaga agacgaaccc ttgcggaaat tccgtccgga cgcactgcgc cagcaacgcg
     5521 tcgcgatctt ccggattcat ggcggctatc aggcggttgc ccggcggaag atcactcata
     5581 ttcggcgaat aaggcgatcc gtgacggccc gtctgtcaaa actcgacaca gcgccacagg
     5641 a
//
LOCUS       DASVVN010000499         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_440248,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000499 DASVVN010000000
VERSION     DASVVN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440248"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VF686_08110"
     CDS             complement(<1..805)
                     /locus_tag="VF686_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8661817.1"
                     /translation="MIDTLTERPSIATVRKPRIGFAGIGWIGRHRMEAMLATGAVEAS
                     VIADPSEAGVADAVARAPGAIAVDGFDALLEHDLDGVVIATPSALHAEQARRALARGV
                     AVFCQKPLGRTEAEVRAVVDAARAADRLLAIDLSYRFTAAATRIRDLVRAGALGRIHA
                     VDLTFHNAYGPDKPWFYDPALSGGGCVMDLGVHLVDLALWLLEDATVTEVSSHLFANG
                     QVLGDHGVAEDYAVATLTLDTGAVIRLACSWRLPAGQDAVIGASLYGDQG"
     gene            complement(802..1788)
                     /locus_tag="VF686_08115"
     CDS             complement(802..1788)
                     /locus_tag="VF686_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8661818.1"
                     /translation="MTDLLDRARTMRAAVITGPGRMEVRETALPVPGAGQVRVRLEGC
                     GVCASNLTPWAGPDWMSFPTEPGGMGHEGWGVVDAIGDGVTAVKVGDRVAALSHHSYA
                     EYDVADEASVVLLPPALDGQPFPGEPLGCAFNIFRRSGIKTGDTVAIVGVGFLGAVLT
                     RLATDAGARVIAISRRPFSLDLAKRFGAAETIPLEDHYGIISRVSELTGGAFCDVVIE
                     AVGKQWPLDLAAELTKERGRLIIAGYHQDGPRQVNMQLWNWRGLDVVNAHERDPAVYA
                     QGVREAVEAVVSGRLGPAPLITHVWPLDRLGEALDATRDRPDGFLKAVVTMR"
     gene            complement(1785..2327)
                     /locus_tag="VF686_08120"
     CDS             complement(1785..2327)
                     /locus_tag="VF686_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8661819.1"
                     /translation="MGSPEQDAGGVEGMSFVLRRNIAEMRERREAAARAAGPSERLSQ
                     AITDFAGSMPFVLLHLLVFGGWILINTLGPEGLRFDPSLVVLAMAASVEAIFLSTFIL
                     ISQNRISAEERRRAELDLQINLLAEHEVTRLIDLVTRIAERLEVPASDPDLDELRRDV
                     APGVVLDTLAEPDETSGDNA"
     gene            complement(2334..>2955)
                     /locus_tag="VF686_08125"
     CDS             complement(2334..>2955)
                     /locus_tag="VF686_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8661820.1"
                     /translation="VYGDPEVHPQPETYRGNVNPIGPRACYDEGKRAAETLTFDYDRA
                     GRGEVRVARIFNTYGPRLSAADGRVVSNVISQALADQPITVFGDGSQTRSFCYVDDLI
                     GGLVALMEHDGTQPGPINLGNPAEMTVSKLVEVVLDLTGSASEVTHQPLPIDDPRRRR
                     PDIARAAEVLGWRPSTPLEEGLRRTIDWFDGERVRTDRNVAAAIGA"
BASE COUNT          454 a         1024 c         1038 g          439 t
ORIGIN      
        1 ctccctggtc gccgtagagg ctggcgccga tgacggcgtc ctgacccgcc ggcaggcgcc
       61 atgagcaggc cagccgaatg acggcgccgg tgtcgagcgt cagggtggcg acggcgtagt
      121 cctcggccac gccatggtca ccgagcacct gaccgttcgc aaacaggtga gaggacacct
      181 ccgtcaccgt cgcgtcctcg agcagccaca gggcgaggtc caccaggtgg acgcccagat
      241 ccatgacgca gccgccgcct gacagcgcgg gatcatagaa ccacggcttg tccggcccgt
      301 aagcgttgtg aaaggtcaga tcgacggcgt ggatccgacc caaggcgccg gcccggacga
      361 ggtcgcggat tcgcgtcgcc gccgcggtga aacgatagct caggtcgatc gccagcagcc
      421 ggtcggcggc gcgcgcagcg tccactacgg cgcggacctc ggcctcggtg cggcccagcg
      481 gcttctggca gaacaccgcg acgccgcgtg cgagggcgcg tcgggcctgt tccgcgtgca
      541 gggcgctggg cgtggcgatg acgacgccgt cgaggtcgtg ttccagcagg gcgtcgaaac
      601 cgtcgacggc tatcgcaccg ggggcccgtg cgacggcgtc ggcgactcct gcttcggagg
      661 ggtcggcgat caccgaggcc tccacggcgc cggtcgcgag catggcttcc attcggtggc
      721 ggccgatcca gccgatgccc gcgaagccga tccggggctt gcgcaccgtg gcgatggacg
      781 gtcgttcagt cagggtgtcg atcatcgcat cgtcaccacg gccttgagga agccgtcggg
      841 ccggtcgcgg gtggcgtcca gcgcctcgcc cagccggtcc agcggccaga cgtgggtgat
      901 cagcggagcg gggccgagcc ggcccgagac cacggcctcg accgcttcgc ggactccctg
      961 cgcatagacg gcagggtcgc gctcatgggc gttgacgacg tccaggccgc gccagttcca
     1021 cagttgcata ttgacctgcc gcggtccgtc ctgatggtag ccggcgatga tcaggcggcc
     1081 ccgctccttc gtcagttcgg cggccaggtc gagcggccat tgtttgccca ccgcctcgat
     1141 gacgacgtcg cagaaggcgc cgccggtcag ttcgctgacg cgggagatga tgccgtagtg
     1201 atcctccagc gggatggtct cagccgcgcc gaaacgcttg gcgagatcga gcgagaaggg
     1261 ccggcgcgag atggcgatca cccgggcccc cgcgtccgtc gccagccggg tcagaacggc
     1321 gccgagaaag cccacgccga cgatcgcgac ggtgtcgccg gtttttatcc cgctgcgccg
     1381 gaagatgttg aaggcgcagc caagcggctc gcccggaaag ggctggccgt cgagtgcggg
     1441 cggcagcagg accacagagg cctcgtccgc cacatcgtat tcggcgtagc tgtggtgcga
     1501 cagcgccgcg acccggtcgc cgaccttcac cgccgtcacg ccgtcgccaa tggcgtcgac
     1561 cacgccccag ccctcatgtc ccatgccgcc gggctcggtc gggaaggaca tccagtccgg
     1621 ccccgcccac ggcgtcagat tggaagcgca tacgccgcag ccctcgagcc tgacccgcac
     1681 ctgaccggcc ccgggcacgg gcagcgcagt ttcgcgcacc tccatccggc cgggtcccgt
     1741 gatcacggcg gcccgcatgg tgcgggcgcg gtcgagaagg tcggtcatgc gttgtctcca
     1801 gatgtttcat caggctcggc gagggtgtcg aggacgacgc cgggcgcgac atcgcggcgg
     1861 agctcgtcca ggtcgggatc gctcgccggg acctcaagcc gctcggcgat gcgggtcacc
     1921 agatcgatca ggcgggtgac ctcgtgttcg gccaacaggt tgatctgcag gtcgagttcg
     1981 gcccggcgcc gctcctcggc cgagatgcgg ttctggctga tcaggatgaa ggtcgacagg
     2041 aagatggcct cgaccgaggc cgccattgcg agaacgacga ggctgggatc gaaccggaga
     2101 ccctcgggac ccagggtgtt gatcagaatc cagccgccga agaccagaag gtgcagcagg
     2161 acgaacggca tgctgccggc gaagtcggtg atggcctgcg acagacgctc gctgggaccg
     2221 gccgcccgcg cggcggcctc gcgccgttcg cgcatctccg cgatgttccg gcgcaggacg
     2281 aacgacatgc cttccacgcc gccggcgtcc tgttccggac tgcccatcgg tcatcaggcg
     2341 ccgatggctg cggcgacgtt gcggtccgtc cgcacgcgct cgccgtcgaa ccagtcgata
     2401 gtccgtctca ggccctcctc gagcggtgtc gacggccgcc agccgagcac ctccgccgcc
     2461 cgcgcgatat cgggcctccg gcggcgcggg tcgtcgatcg gcaggggctg gtgcgtcact
     2521 tccgacgccg agccggtcag gtcgagcacg acctcaacca gcttcgatac ggtcatctcg
     2581 gccgggttgc ccagattgat gggaccgggc tgcgtcccgt catgctccat aagggcgacc
     2641 aggccgccga tcaggtcgtc gacgtagcag aaggagcggg tctggctgcc gtctccgaat
     2701 acggtgatcg gctggtccgc cagcgcctgg ctgatgacgt tcgacaccac acgcccatcg
     2761 gccgcggaca ggcgcgggcc ataggtgttg aatatccggg ccacccgcac ctcgccccga
     2821 cccgcccggt cgtagtcgaa ggtcagggtc tccgccgccc gcttgccctc gtcatagcag
     2881 gcgcgcggcc cgatcggatt gacgttgccg cggtacgtct cgggctgcgg atgcacttcc
     2941 ggatcgccgt acacc
//
LOCUS       DASVVN010000500         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_202505,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000500 DASVVN010000000
VERSION     DASVVN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202505"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VF686_08130"
     CDS             <1..449
                     /locus_tag="VF686_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX8661821.1"
                     /translation="SNGYSLIRKVVEKSGLSWDADAPFAKDRTLAQALMEPTRIYVKP
                     VLPLMKAGLVKGAAHITGGGLIENPPRCIADGLQASFDWDAWPVPQVFQWLGEVGGIS
                     DHELRRTFNCGVGFILIVAPENAEPVLEALLNAGEVAFVCGQLVAA"
     gene            complement(446..904)
                     /locus_tag="VF686_08135"
     CDS             complement(446..904)
                     /locus_tag="VF686_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661822.1"
                     /translation="MLALVLAAVLTASAPPTDITRTILSDVCLPYVAGEARDAASLDP
                     LGFVATAADEDTLNFQSGDQAYILRLTTTGSVADDNLNRVCVIQARRGDFDRTRQAIQ
                     PLLREQGFTPEADLPTGRAIWTKGGVTVSLRQNPGAAAIVRVTYSTLDAG"
     gene            1029..2408
                     /locus_tag="VF686_08140"
     CDS             1029..2408
                     /locus_tag="VF686_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8661823.1"
                     /translation="MRVLSVALAGLVLSASAASAQAPVQEQALLHQLAGDIQPARMRA
                     DIEALVGFGTRHTMSETQSDTRGIGAARRWAQGQFEAISRDCGGCLTVVTPSDTVTAA
                     RVPTPTEVVDIVAIQRGTGDPNRVIIISGHIDSRVTDVMNFTADAPGANDDASGVAAV
                     LEAARVLSKHKFDATLVFAALSGEEQGLLGGKILADYAQAQGWRVEANLNNDIVGNSE
                     GQSGVRDTTRVRVFSEGTKTVETEAQEERRRYNGGEVDSSSRNLARFVDGIADRYLAN
                     FDVEMIYRTDRYGRGGDQVEMLRHGFPAVRISEGAENYDRQHQDLRTENGVEYGDTIE
                     GVDFDYLGQVARLNIVSMAALANAPASPLNVTIEGAVKPDTTIKWQAVPGAAGYRVWW
                     RSTTAPQWTHSRWAGSATELVLRNIVIDDYFFGVSAVSDDGYESPVVFPGPAGSFVSV
                     GDPAPRPAN"
     gene            2408..3004
                     /gene="purN"
                     /locus_tag="VF686_08145"
     CDS             2408..3004
                     /gene="purN"
                     /locus_tag="VF686_08145"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX8661824.1"
                     /translation="MPDIRPVRVAVLISGAGSNMAALIDAGQQADARFEVVLVLSNKP
                     DAGGLAVAQAKGVATATVDHRPFGADRQAHEQALDAALTAHDVEVVALAGYMRLLTPS
                     LVGRWTGRMLNIHPSLLPKYPGLDTHARAIAAGDAEAGCSVHIVTEGVDEGPVLGQSA
                     VPVQADDTPASLAQRVLAAEHALYPRALADFCRQIRSL"
     gene            3076..>3951
                     /locus_tag="VF686_08150"
     CDS             3076..>3951
                     /locus_tag="VF686_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8661825.1"
                     /translation="MLRIRLMACAAGAALTLAAGIAQAQTAPPIVEIPSATQAEIGEF
                     GFDIGGMDTTIRPGADFNQYANGTYLKNTPIPADRTSYGAFHMLHDRSQENLRTLIEE
                     SAANPTTSPDAARIGAFYGSFMDEATVERLGASPLQADLAEVRAADTREEIAALMGRT
                     QGGFGSSLFGAQIFEDLKDPKTIAVYVGQGGIGMPDRDYYLEESFATQKTAYQAYVAK
                     ALELAGWPNYEQAAADVVAFETRIAEQHWTQVQNRQIDKIYNPATLAELAAAAPGFDW
                     AAWAQGAGIGHVPTLI"
BASE COUNT          632 a         1477 c         1264 g          578 t
ORIGIN      
        1 actcgaatgg ctactcgctg atccgcaagg tggtcgagaa gtccggcctg tcatgggacg
       61 ccgacgctcc cttcgccaag gaccgcaccc tggcccaggc gctgatggag ccgacgcgca
      121 tctatgtgaa gccggtgctg ccgctgatga aggccgggct ggtgaagggc gcggcccaca
      181 tcaccggcgg cggtctgatc gagaacccgc cccgctgcat cgccgacggt cttcaggcca
      241 gcttcgactg ggacgcctgg cccgtgccgc aggtgttcca gtggctgggc gaggtcggcg
      301 gcatctcgga tcacgaactg cgccgcacct tcaactgcgg cgtcggcttc atcctgatcg
      361 tcgcgcccga gaacgccgag ccggttctgg aagccttgct gaacgccggc gaggtcgcct
      421 tcgtctgcgg ccagctggta gccgcctagc ccgcgtccag cgtgctgtag gtcacccgga
      481 cgatcgccgc cgcgccgggg ttctggcgca gcgacaccgt caccccgccc ttggtccaga
      541 tcgcccgacc ggtcggcagg tccgcctccg gggtgaaccc ctgctcgcgc agcagcggct
      601 ggatcgcctg tcttgtgcga tcgaagtcgc cacgccgcgc ctggatgacg cagacccggt
      661 tgaggttgtc gtccgcgacg ctgcccgtcg tggtcagccg caggatgtag gcctggtccc
      721 cgctctggaa attgagggtg tcctcgtcgg ccgccgtggc gacgaaaccc agcggatcca
      781 gcgaggccgc atcgcgagcc tcccccgcga catagggcag gcacacgtcg ctcaggatgg
      841 tccgcgtgat atcggtcgga ggcgctgaag ccgtcaggac ggcggcgagg acgagagcga
      901 gcatcaggaa cctccggaga gtccgccact gtttcccacc tccgggccgt acgcaagcgg
      961 ctgcgcgccg ttgacccgac ggggcccgcc cggcaaggtc cggccactcc ctgtaaacga
     1021 ggtcgccgat gcgtgtgctt tctgtcgccc tggccgggct ggtgctttcg gcatcggccg
     1081 cgtcggccca ggctccggtt caggaacagg ccctgctgca ccagctggcc ggcgacattc
     1141 agcccgcgcg gatgcgcgcg gacatcgagg ccctggtcgg cttcggcacc cgccacacca
     1201 tgtccgaaac gcagtcagat acgcgcggca tcggcgcggc gcggcgatgg gcgcagggcc
     1261 agttcgaggc gatcagccgc gactgcggcg gctgcctgac cgtcgtcacc ccttcggaca
     1321 cggtcaccgc cgcccgcgtc cccaccccga ccgaggtcgt cgacatcgtc gcgatccagc
     1381 gcggcacggg cgatccgaac cgggtcatca tcatctcggg ccatatcgat tcgcgcgtca
     1441 ccgacgtgat gaacttcacc gccgacgccc ccggagccaa tgacgacgcc tcgggcgtcg
     1501 ccgcggtcct cgaggccgcg cgggtgctgt cgaagcacaa gttcgacgcc accctggtgt
     1561 tcgccgccct ctcgggcgag gaacagggcc tgctgggcgg caagatcctc gccgactacg
     1621 cccaggcgca gggctggcgg gtcgaggcca atctcaacaa cgacatcgtc ggcaattccg
     1681 agggccagtc cggcgtgcgc gacacgaccc gggtccgcgt cttctcggaa ggcaccaaga
     1741 ccgtcgagac cgaggcccag gaagagcgcc ggcgctacaa cggcggcgag gttgattcct
     1801 cgtcccgcaa cctcgcccgc ttcgtcgacg ggatcgccga ccgctatctg gccaatttcg
     1861 acgtcgagat gatctatcgc acggaccgct acggccgcgg cggcgatcag gtcgagatgc
     1921 tgcgccacgg cttccccgcc gtccgcatct cggagggcgc cgagaactat gaccgccagc
     1981 atcaggacct gcgcaccgaa aacggcgtcg aatacggcga caccatcgaa ggcgtggact
     2041 tcgattatct gggtcaggtc gcgcggctga acatcgtctc catggccgcc ctcgccaacg
     2101 ccccggccag cccactgaac gtgaccatcg aaggcgcggt caagcccgac accacgatca
     2161 aatggcaggc cgtgcccggc gcggcgggat atcgcgtctg gtggcggtcg accaccgccc
     2221 cgcaatggac ccacagccgc tgggccggat cggcgaccga gctggtgctg cggaacatcg
     2281 tcatcgacga ctatttcttc ggcgtctcgg ccgtgtcaga cgacggctat gagagcccgg
     2341 tcgtcttccc aggcccggcc gggtccttcg tctcggttgg cgacccggcg ccccgcccgg
     2401 ccaactgatg cccgacatcc gtcccgtccg cgtcgccgtc ctcatctccg gcgcgggctc
     2461 caacatggcc gcgctgatcg acgccggtca gcaggcggac gcccggttcg aggtcgtgct
     2521 ggtcctgtcc aacaagccgg acgccggcgg cctggcggtg gcgcaggcca agggcgtcgc
     2581 gaccgcgacg gtcgaccatc gccccttcgg cgcggaccgt caggcgcacg agcaggccct
     2641 cgatgccgcc ctgaccgccc atgacgtcga agtcgtggcg ctggccggct atatgcggct
     2701 gctgacgccc tcgctggtgg gccgttggac cggccggatg ctgaacatcc accccagcct
     2761 gctgcccaaa taccccggcc tcgacaccca cgcccgcgcc atcgccgccg gcgacgccga
     2821 ggccggctgc tccgtgcaca tcgtcaccga aggcgtcgac gagggccccg tgctgggtca
     2881 gtccgccgtg ccggtacagg ccgacgacac gcccgcctcc ctggcccagc gcgtgctggc
     2941 cgccgaacac gccctctacc cccgggcgct ggccgacttc tgccgccaga ttagaagttt
     3001 gtaataaccc gtctttcccg gcgacagcgg cgatgacctg aggcagcttg ccgcccaatt
     3061 tcaacggggg ctttcatgct cagaattcgt cttatggcct gcgccgccgg cgccgccctt
     3121 acgctcgccg ccggcatcgc ccaggcccag accgccccgc cgatcgtgga aatcccctcg
     3181 gcgacccaag ccgagatcgg cgaattcggc ttcgacatcg gcggcatgga caccaccatc
     3241 cgccccgggg ccgacttcaa ccagtacgcc aacggcacct atctgaagaa caccccgatc
     3301 cccgccgacc gcacctcgta cggcgccttc cacatgctgc acgaccggtc gcaggagaac
     3361 ctgcgcaccc tcatcgagga aagcgccgcc aacccgacga ccagccccga cgccgcccgc
     3421 atcggcgcct tctacggcag cttcatggac gaggcgacgg tcgagcgtct cggcgcctcg
     3481 cccctgcagg ccgatctggc cgaggtccgc gccgccgaca cgcgcgaaga gatcgccgcc
     3541 ctgatgggcc gcacccaggg cggcttcggc tccagcctgt tcggcgccca gatcttcgag
     3601 gacctgaagg acccgaagac catcgccgtc tacgtcggcc agggcggcat cggcatgccc
     3661 gaccgcgact actatctcga agaaagcttc gcgacccaga agaccgccta tcaggcctat
     3721 gtcgccaagg cgctggaact ggccggctgg cccaactacg agcaggccgc cgccgacgtc
     3781 gtcgcgttcg agacgcgcat cgccgaacag cactggaccc aggtccagaa ccgccagatc
     3841 gacaagatct acaatcccgc caccctcgcc gaactggccg ccgcagcccc cgggttcgac
     3901 tgggcggcct gggcccaggg cgccggcatc ggccacgtcc cgaccctgat c
//
LOCUS       DASVVN010000501         5409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_333932,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000501 DASVVN010000000
VERSION     DASVVN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333932"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VF686_08155"
     CDS             <1..1411
                     /locus_tag="VF686_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8661826.1"
                     /translation="RLLDPGSPFLEVGALAANDMYRGEAPGAGVIGGIGRVSGRECMI
                     LANDPTVKGGAYFPMTVKKHLRAQEIARENRLPCIYLVDSGGANLPHQAEVFPDREHF
                     GRIFFNQAQMSADGIAQIACVMGSCTAGGAYVPAMSDETVIVRNQGTIFLAGPPLVKA
                     ATGEVISAEELGGAETHGRRSGVVDYVAENDEHALEIVRTIVDSLNTVKRIELDVREP
                     RQPAFDAEELYGLIPDDVRAPYDVREVIARLVDGSEFDEFKALYGTTLVCGFARIWGY
                     PVAILANNGVLFSESALKGAHFIELACKRKIPLVFLQNISGFMVGGKYEAEGIAKNGA
                     KLVTAVASAEVPKFTVLIGGSFGAGNYGMCGRAYSPRFLFTWPNSRISVMGGEQAAAV
                     LATVHRDADKWTPEEAEAFKAPVRQKYEDEGNPYYATARLWDDGIIDPVQTRDVLGLA
                     ISASLNAPIPETTFGVFRM"
     gene            complement(1401..1673)
                     /locus_tag="VF686_08160"
     CDS             complement(1401..1673)
                     /locus_tag="VF686_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661827.1"
                     /translation="MKAKLAAEIRQQLMDQGGAAHRDVVIGRILHRKGLSGLAADRAR
                     RALLSAFELHADAAKECGAPHLFELPFGPESHRWALDGTVRPPLTS"
     gene            1858..2757
                     /locus_tag="VF686_08165"
     CDS             1858..2757
                     /locus_tag="VF686_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8661828.1"
                     /translation="MTDKPTEADLNALDITDAEAAEINAISHPLLADPAPDANDDLVQ
                     IDSTADGVVFVTINRPDKRNAFDAPTIAALYEAFETLHGADNVRLVFVRGAGGTFSAG
                     ADLGWMRDAAGWSESDNRDDAMGLAKMLKALHDVPALTCAIVEGAAMGGGAGIVAACD
                     MAVAVEGARFAFSEVKLGLIPATIAPYVVEAVGARRARQLFLSANIFDADYAAHAGLI
                     DMVLPEGSVDEFISMLTDSISANAPGAMGDAKRLVNDVVGHRIDHGLMEDTAKRIARA
                     RVSVEGQEGVRAFLEKRKPAWAQ"
     gene            2777..3895
                     /locus_tag="VF686_08170"
     CDS             2777..3895
                     /locus_tag="VF686_08170"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX8661829.1"
                     /translation="MSGELETAAVASAGGWLKWKREHADIPVGTPCANCETPLAGPHC
                     HECGQLAEDFHKSVWKLTVEAVESLLHLDGRLFSTLPNLIRRPGKLTRDYMEGKRASQ
                     VAPVRMFLVILLIAFFVGHAAMKAGAKHEAAGDDHAAPATVGAAPTFKYNGRVVTPGS
                     ATMAEFEREVMADPDLSAAEKRTQIAAARGDWASFSRNLAANVAEETEANAAEAPKME
                     DGDDAEKLRGFKEWIETRQAAIKDDPERFALILEIWAHRVAILALPVSALMLTLLFAF
                     NRRFYVFDHVIFSMHSLSFQLLLLILIMALGVLTPLAWWLLWLAPVHLFLHMKGAYQR
                     SVFGTLARMFVLFTLTTVTFSLLALLWLYLGFNEMAGH"
     gene            4066..>5409
                     /locus_tag="VF686_08175"
     CDS             4066..>5409
                     /locus_tag="VF686_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX8661830.1"
                     /translation="MFKSVLVANRGEIACRVFRTARRMGIRTIAVYSEADAKALHVRE
                     ADEAVLIGSAPARESYLDAAKVLAAAKTKGAEAIHPGYGFLSENADFAEAVTAAGLVW
                     IGPPPAAIRAMGLKDAAKTLMIEAGVPVTPGYQGEDQSLETLTAEAARIGYPVLIKAV
                     AGGGGKGMKKVDRAEDFADGLASAQREGQSSFGDPRVLIESYITRPRHIEVQVFGDSH
                     GAAVHLYERDCSLQRRHQKVIEEAPAPGMDAATRKAVTDAAVRAARAVNYEGAGTIEF
                     IADASDGLKADRIWFMEMNTRLQVEHPVTEAVTGVDLVEWQFRVAAGEPIPLKQEDIP
                     LNGWAMEARLYAEDPANGFLPSIGKLEHFDLPGPDRAHRSVDVRVDTGVEEGGEVSQF
                     YDPMIAKLIVHADSREEAARRLADVCGQVEVWPVKANAGFLKRCLEHPRFVAGDVD"
BASE COUNT          897 a         1909 c         1775 g          828 t
ORIGIN      
        1 ccgcctgctc gatccgggct cgcccttcct cgaggtcggc gcgctggcgg cgaacgacat
       61 gtaccggggc gaggcccccg gcgcgggcgt gatcggcggc atcggccggg tctcggggcg
      121 cgagtgcatg atcctcgcca atgacccgac ggtgaagggc ggcgcctatt tcccgatgac
      181 ggtgaagaag catctgcgcg cgcaggagat tgcgcgcgag aaccggctgc cgtgcatcta
      241 tcttgtcgac agcggcggcg ccaacctgcc gcaccaggcg gaggtgtttc cggacaggga
      301 gcatttcggg cggatattct tcaaccaggc gcaaatgtcg gcggacggga tcgcccagat
      361 cgcctgcgtg atggggagct gcaccgcggg cggagcctat gtgccggcca tgtcggacga
      421 gaccgtcatc gtccgcaatc agggcaccat cttcctcgct ggaccgccgc tggtgaaggc
      481 cgcgaccggc gaggtcatct cggccgagga gctgggcggc gccgagaccc acggccgccg
      541 gtcgggcgtg gtcgactatg tcgccgagaa cgacgagcac gcgctggaga tcgtccgcac
      601 catcgtcgac agcctgaaca cggtgaagcg cattgaactg gacgtgcgcg agccgcgcca
      661 gcccgctttc gacgccgagg agctttacgg cctgatccct gacgacgtcc gcgcgcccta
      721 cgacgtgcgc gaggtcatcg cgcgtctggt ggacggctcg gaattcgacg agttcaaggc
      781 gctgtacgga accaccctgg tctgcggctt cgcccgcatc tggggctatc cggtcgccat
      841 cctcgccaac aacggcgtgc tgttctcgga gagcgcgctg aagggcgccc acttcatcga
      901 actggcctgc aagcggaaga tcccgctggt cttcctgcag aacatctcgg gcttcatggt
      961 gggcggcaaa tacgaggcgg agggcatcgc caagaacggc gccaagctgg tcaccgccgt
     1021 cgcctcggcc gaggttccca agttcaccgt tctgatcggc ggcagcttcg gggcgggcaa
     1081 ctacggcatg tgcggccgcg catatagccc gcgcttcctc ttcacctggc ccaacagccg
     1141 gatcagcgtc atgggcggcg aacaggccgc cgccgtcctg gccaccgtcc accgcgacgc
     1201 cgacaaatgg acgcccgagg aggccgaggc gttcaaggcc ccggtgcggc agaaatacga
     1261 ggacgagggc aacccctact acgccaccgc ccggctgtgg gacgacggca tcattgatcc
     1321 ggtgcagacc cgcgacgtgc tgggtctggc catctccgcc tcgctgaacg ccccgattcc
     1381 ggagacgacc ttcggcgtgt tcaggatgta agcggcggcc ttaccgttcc gtccagcgcc
     1441 cagcggtggg actccggtcc gaacggcagc tcgaacagat gcggcgcgcc gcactccttc
     1501 gccgcatcgg catgcagttc gaaggcggac agcagcgccc gccgcgcgcg gtcggcggcc
     1561 aggccgctga ggcccttgcg gtgcaggatg cgtccgatga ccacgtcacg gtgggcggct
     1621 ccgccctgat ccatcagctg ttgccggatc tcggcagcca gcttcgcctt cacgggagtc
     1681 agccgcgcgg gtttccggga tcgcgtgaca tgcggcatgg ggtcctcgca gctcctgacc
     1741 tgcagcgatc aattcccccg ttccggtcgc tgtcaagaaa cgagcccggc atcggcgatc
     1801 cgtcgctgcc attcggccgg ccatgcgtta ctgttggcca actccgaagg accgaccatg
     1861 accgacaagc cgaccgaagc cgacctcaac gccctcgaca tcaccgacgc cgaggcggcc
     1921 gagatcaacg ccatctcgca tccgctgctg gccgacccgg cgccggacgc caacgacgat
     1981 ctggtccaga tcgactccac cgccgacggc gtggtcttcg tcaccatcaa ccggccggac
     2041 aagcgcaacg ccttcgacgc cccgaccatc gccgccctgt acgaggcgtt cgagactctg
     2101 cacggggccg acaatgtgcg tctggtcttc gtgcgcggcg cgggcgggac gttcagcgcc
     2161 ggcgccgatc tgggctggat gcgtgacgcg gccggctggt cggaaagcga caaccgcgac
     2221 gacgccatgg gtctggcgaa gatgctgaag gccctgcacg acgtcccggc cctgacctgc
     2281 gccatcgtcg agggcgcggc catgggcggc ggggccggca tcgtcgccgc ctgcgacatg
     2341 gccgtggccg tcgagggggc gcgcttcgcc ttctcggagg tcaagctggg cctgatcccc
     2401 gcgaccatcg ccccctatgt cgtcgaggcc gtcggggccc gccgcgcccg ccagctgttc
     2461 ctgtccgcca acatcttcga cgccgactac gcggctcacg ccggcctgat cgacatggtc
     2521 ctgcccgagg gctcggtcga cgaattcatc tccatgctga ccgacagcat cagcgccaat
     2581 gcgcccggcg ccatgggcga cgccaaacgg ctggtcaacg acgtcgtcgg tcacaggatc
     2641 gaccacgggc tgatggaaga cacggcaaaa cgcatcgccc gcgcccgcgt ttccgtcgag
     2701 ggccaggagg gggtgcgcgc cttcctcgaa aaacgtaaac cagcctgggc tcagtaagaa
     2761 tcgcaggggg tgtccgatgt cgggggaact ggaaacggcc gcggtcgcct cggccggcgg
     2821 ctggctgaag tggaagcgcg agcacgcgga tatcccggtc ggcacgccct gcgccaactg
     2881 cgaaaccccg ctggccgggc cgcactgcca tgaatgcggc caactggccg aggacttcca
     2941 caaatccgtc tggaagctga ccgtagaggc ggtcgaaagc ctgctgcacc tcgacggccg
     3001 gctgttcagc accctgccca atctgatccg caggcccggc aagctgaccc gcgactatat
     3061 ggagggcaaa cgcgcctcac aggtcgcgcc ggtccgcatg ttcctggtca tcctgctgat
     3121 cgccttcttc gtcggccacg cggccatgaa ggccggcgcc aagcatgaag ccgccgggga
     3181 tgatcacgcc gcccccgcca ccgtcggggc ggctccgacg ttcaaataca acggccgcgt
     3241 cgtcacgccc ggcagcgcca cgatggccga gttcgagcgc gaggtcatgg ccgaccccga
     3301 tctgagcgcg gccgagaaac ggacccagat cgcagccgcc cgcggcgact gggccagctt
     3361 cagccgcaac ctcgccgcca atgtcgccga ggagaccgag gccaacgccg ccgaggctcc
     3421 gaagatggag gacggggacg acgccgagaa actgcgcggc ttcaaggaat ggatcgagac
     3481 ccgtcaggcg gcgatcaagg acgaccccga gcggttcgcc ctgatcctcg agatctgggc
     3541 ccaccgcgtc gccatcctgg ccctgccggt gtcggccctc atgctgaccc tgctcttcgc
     3601 cttcaaccgg cgcttctacg tcttcgacca cgtcatcttc tcgatgcact cgctgtcgtt
     3661 ccagctgctg ctactgatcc tgatcatggc gctgggcgtg ctgacgccgc tggcctggtg
     3721 gctgctgtgg ctggcccccg tgcacctgtt cctgcacatg aagggcgcct atcagcgcag
     3781 cgtcttcggc accctggcgc ggatgttcgt cctgttcacc ctgaccaccg tcaccttcag
     3841 cctgctggcg ctcctgtggc tctacctcgg gttcaacgag atggctggac actaggcgcg
     3901 gccgcgctag tggctagtgg ctagtggcta ggggctaggg gctaggggct aggggctgat
     3961 attcgcgcgt ctgacgcacg caacacctcg cttctgggcg caggcagcaa tgcggcgatc
     4021 actagccact agccactagc cactagccac ctctacaacc cacccatgtt caaatccgtc
     4081 ctggtcgcca atcgcggcga gatcgcctgc cgcgtcttcc gcaccgcccg ccgcatgggc
     4141 atccgcacca tcgccgtcta ttccgaggca gacgcgaagg ccctgcatgt gcgcgaggcc
     4201 gacgaggcgg tgctgatcgg ctcggccccg gcgcgcgaaa gctatctgga cgctgcgaag
     4261 gtcttggccg ccgccaagac caagggcgcc gaggccatcc accccggcta tggcttcctg
     4321 tccgagaacg ccgacttcgc cgaggccgtg accgccgccg gtctggtctg gatcggcccc
     4381 ccgcccgccg cgatccgcgc catggggctg aaggacgcgg ccaagacact gatgatcgag
     4441 gccggggtgc cggtcacgcc cggctatcag ggcgaggatc agtcgctcga aaccctgacc
     4501 gccgaagccg cgcgcatcgg ctatcccgtc ctgatcaagg ctgtcgccgg cggcggcggc
     4561 aagggcatga agaaggtcga ccgcgccgag gatttcgccg acggcttggc ctcggcccag
     4621 cgcgagggcc agtcgtcgtt cggcgacccg cgggtcctga tcgagagcta tatcacccgc
     4681 ccccgccaca tcgaggtgca ggtcttcggc gacagccacg gcgccgccgt ccacctctac
     4741 gagcgcgact gctccctgca gcgccgccac cagaaggtga tcgaggaggc cccggccccg
     4801 ggcatggacg cggccacccg caaggccgtc accgacgccg ccgtccgcgc cgcccgcgcc
     4861 gtgaactacg agggcgccgg cacgatcgaa ttcatcgccg acgcctcgga cgggctgaag
     4921 gccgaccgca tctggttcat ggagatgaac acccggctgc aggtcgagca cccggtcacc
     4981 gaggccgtca ccggcgtcga tctggtcgaa tggcagttcc gcgtcgccgc cggcgagccc
     5041 atcccgctga aacaggagga catccccctg aacggctggg ccatggaggc gcggctgtac
     5101 gccgaggatc ccgccaacgg cttcctgccc agcatcggca agctcgagca tttcgacctg
     5161 cccggacctg atcgcgctca tcgatcggtc gatgtgcgtg tcgacacggg tgtggaggaa
     5221 ggtggcgagg tcagccagtt ctacgacccg atgatcgcca agctgatcgt ccacgcagac
     5281 agccgtgagg aagccgcgcg acgcctcgcg gacgtctgcg gccaagtcga ggtctggccc
     5341 gtgaaggcca acgccggctt cctgaaacgc tgcctcgaac atccccgctt cgtcgccggt
     5401 gatgtcgac
//
LOCUS       DASVVN010000502         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_466115,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000502 DASVVN010000000
VERSION     DASVVN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466115"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1117
                     /locus_tag="VF686_08180"
     CDS             206..1117
                     /locus_tag="VF686_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8661831.1"
                     /translation="MAESVERRQGRLSRRGLLSGVAVLAVAGLAGCGREEKTAEPPAT
                     PDGPPKGSLEWAVAGPWRAQDKARDAARHPMETLRFFGLQPRMTVVEFWPGSGWYTEI
                     LAPYLAEGAGKYVAAGFQTGPGADPSQVQLAAAFQQRFTTDKRLYGEIELSAFGPTSG
                     PVTEAGTADMVLFMRNIHAWMAAGIAEKAFADAWAALRPGGVLGVEQHRLGPEEDQDP
                     AAANGYVQEAFVRQLAAEAGFAFVAASEINANERDTKDHPFGVETLPPSSLTAPRGSP
                     PNPAFDRSKYDRIGESDRMTLRFRKPE"
     gene            1114..>1560
                     /locus_tag="VF686_08185"
     CDS             1114..>1560
                     /locus_tag="VF686_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661832.1"
                     /translation="MNRAVAYAANAPLMGVPGASAPPGGIGEWFRGAGGLRLRAAFWT
                     PSALIAKKPRGTVIVSPGRTEPIEKYYEVIGNFLARGWCVLAHDWRGQGLSARLLPDR
                     LKGHARAVEEFLDDYARLLDTWEARAPKPWVMVGHSMGGVLNLMTLE"
BASE COUNT          251 a          573 c          512 g          224 t
ORIGIN      
        1 aacgaccgtc ccacgcccct caccgaagcc gagcgggccg agtatgacgc gatcggcgag
       61 agcgaccgca tgaccctgcg attccgcaag cctgcgtgac cacagatcat gcgggaaacc
      121 gttttgacgg gctggaggaa cacggttaag ccctgcgtgc cggggcggct cctgatcgga
      181 caggaccgct ctgcggggaa tgtgcatggc tgaatccgtc gaacgccgcc aaggtcgcct
      241 gtcgcgccgc ggcctgctgt ccggcgtcgc cgttctcgcc gtcgccggcc tcgccggctg
      301 cggccgcgag gaaaagaccg ccgaaccgcc cgccaccccc gatggcccgc ccaagggctc
      361 gctggaatgg gctgtcgccg gtccgtggcg cgcccaggac aaggctcgcg acgccgcccg
      421 ccatccaatg gagacgctgc gcttcttcgg ccttcagccg cgcatgacgg tggtcgaatt
      481 ctggcccggc agcggctggt acaccgagat tctggcacct tatctggccg agggcgcggg
      541 caaatacgtc gccgccggct tccagaccgg ccccggcgcc gatccctcgc aggtccagct
      601 ggccgccgcc ttccagcagc gcttcaccac cgacaaacgc ctgtacggcg agatcgagct
      661 cagcgccttc ggccccacct cgggccccgt gaccgaggcc ggcacggccg acatggtcct
      721 gttcatgcgc aacatccacg catggatggc cgccggcatc gccgaaaagg ccttcgccga
      781 cgcctgggcc gccctgcgtc ccggcggcgt cctcggcgtc gaacagcacc gcctcggccc
      841 cgaggaagat caggaccccg ccgcggcgaa cggatacgtc caggaagcgt tcgtcagaca
      901 gctggcggcg gaagcgggct tcgcctttgt cgcagcgtcg gaaatcaacg cgaatgaaag
      961 ggataccaag gatcatccgt tcggggtcga aaccctgccg ccctcgtcgc tgaccgcccc
     1021 gcgcggttcg ccgccgaacc cggccttcga ccggtcgaag tacgaccgga tcggcgagag
     1081 tgaccgcatg accttgagat tcaggaagcc agagtgaacc gagccgtcgc ctacgccgcc
     1141 aacgcacccc tgatgggggt tccgggcgcc tccgcgcctc cgggcgggat cggcgagtgg
     1201 ttccgcggcg ccggcggtct gcgcctgcgc gccgccttct ggacgccttc ggccctgatc
     1261 gcgaagaaac cgcgcggcac ggtgatcgtc agccccggcc gcaccgagcc gatcgagaaa
     1321 tactacgaag tcatcggtaa tttcctcgcc cgcggctggt gcgtcctcgc ccacgactgg
     1381 cgcggccagg gcctctcggc ccgcctgctg cccgatcgcc tcaagggcca cgcccgggcg
     1441 gtggaggaat tcctcgacga ctacgcccgc ctgctggaca catgggaggc gcgcgcgccg
     1501 aagccctggg tcatggtcgg ccattcgatg ggcggcgtcc tcaacctgat gacgctggaa
//
LOCUS       DASVVN010000503         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_333989,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000503 DASVVN010000000
VERSION     DASVVN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333989"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2025)
                     /locus_tag="VF686_08190"
     CDS             complement(160..2025)
                     /locus_tag="VF686_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX8661833.1"
                     /translation="MKRTLMTATAVCALALGSAACAGLGMNPGEAAEIVPAPTSARPP
                     ATAESAIAFVADAETRLAALSEEAARIQWTRATNITHDTMWLESRINAEYTELQVELA
                     NGAAQFNDVQVPEDVRRKLNLLRLGIVLPAPNRPGAAGELAEITTRLDSTYSVGKFDF
                     KGRQITLDDATTLIAESRDPAETKALYEGWRTISPVMAPDYARMVEIANEGSRELGFA
                     DTGAMWRAGYDMEPDAFAAETDRLWTQVKPFYENLHCYVRGRLNAKYGDAVQPDSGPI
                     RADLLGNMWSQQWGNIYDVVAPAEGGASSYDLTERLVAANYDAKKMVETGEGFYVSMG
                     LPELPETFWERSMITRPRDREVVCHASAWDLDNLEDIRIKMCTQVNGDDFYTVHHELG
                     HNYYQRAYRNQPFLFKNGANDGFHEAIGDFVGLSALTPTYLNQIGLLQTVPGAEEDIP
                     FLLKMALDKIAFLPFGLMVDRWRWGVFNGETSPAEYNDAWSANMLKYQGLVPPGPRPA
                     NAFDPGAKYHIPGNTPYTRYFLAHIYQFQFQRAACKEAGWTGPLHRCSIYGNKEVGER
                     FERMMEMGQSRPWPEAMQAFTGESGNDASAVTDYFAPLNAWLTIQNRGKDCGWDA"
     gene            complement(2105..>2608)
                     /gene="mtgA"
                     /locus_tag="VF686_08195"
     CDS             complement(2105..>2608)
                     /gene="mtgA"
                     /locus_tag="VF686_08195"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX8661834.1"
                     /translation="AVIASEDSTFCAHRGFDMKAIEKALKANARSEKRGGNRIRGGST
                     ISQQTAKNAFLWPGRDWIRKGLEAGYTVAIETVWGKRRIMEVYLNVAEWAPGVYGAEA
                     ASRHWFGKSADDLTAREAARLAAILPSPRRYDAASPGPYVRRRTSRIQAAMGTVRNDG
                     LASCVLG"
BASE COUNT          391 a          813 c          931 g          473 t
ORIGIN      
        1 ccggctcacg ccgctgatcc aggtattcag ccccatcgct gcatccgccc ttggccgcag
       61 acgcaattcg cccacgctca tccggcggag aatgaagcaa ggcggttaag agaaggtcca
      121 acgccgacgg ttaacagaac cgccggccgg agccatgggt caggcgtccc agccgcagtc
      181 cttgccgcgg ttctggatgg tcagccaggc gttcagcggc gcgaaatagt cggtcaccgc
      241 cgaggcgtca ttgccgcttt cgcccgtaaa ggcctgcatg gcttcgggcc acggacgcga
      301 ctggcccatt tccatcatcc gctcgaagcg ctcgccgacc tccttgttgc cgtagattga
      361 gcagcggtgc agcggtccgg tccagccggc ttccttgcag gccgcgcgct ggaactggaa
      421 ctgatagatg tgggccagga agtagcgggt gtacggcgta ttgcccggga tatggtattt
      481 ggcgcccggg tcgaaggcgt tggcaggtcg cggtccgggc ggaacaaggc cctgatattt
      541 cagcatgttg gccgaccagg cgtcgttgta ttccgccggg ctggtctcgc cgttgaagac
      601 gccccagcgc cagcggtcga ccatcagacc gaatggcagg aaggcgatct tgtccagcgc
      661 catcttgagc aggaagggga tgtcctcttc ggcgcccggc acagtttgca gcagaccgat
      721 ctggttcaga taggtcggcg tcagggccga caggccgacg aaatcgccga tggcttcgtg
      781 gaagccgtcg ttcgctccgt tcttgaacag gaagggctga ttccggtagg cgcgctgata
      841 gtagttgtga cccagctcgt ggtggacggt gtagaagtcg tcgccgttca cctgggtgca
      901 catcttgatg cggatgtcct cgaggttgtc gaggtcccag gccgaggcgt ggcagaccac
      961 ctcgcggtcg cgcggccggg tgatcatcga ccgctcccag aaggtttccg gcaactcggg
     1021 caggcccatc gagacgtaga agccctcgcc cgtctcgacc atcttcttcg catcgtagtt
     1081 ggcggcgacc agccgttcgg tcaggtcgta gctcgaggcg ccgccctcgg ccggggcgac
     1141 cacgtcatag atattgcccc actgctggga ccacatattg cccaacaggt cggcgcggat
     1201 cgggccgctg tccggctgga ccgcatcgcc gtatttggcg ttcaggcgtc cgcgcacata
     1261 gcagtgcagg ttctcgtaga acggcttgac ctgggtccac agccggtcgg tctcggcggc
     1321 gaaggcgtcc ggctccatgt catagccggc acgccacatg gcgcccgtgt cggcgaagcc
     1381 cagttcgcgg ctgccctcgt tggcgatctc gaccatgcgg gcgtagtcgg gggccatgac
     1441 cggcgagatg gtgcgccagc cctcgtaaag ggctttggtc tcggccggat cgcggctttc
     1501 ggcgatcagg gtggtggcgt cgtcgagggt gatctggcgg cccttgaagt cgaacttgcc
     1561 gaccgaatag gtggaatcga gacgggtggt gatctcggcc agttcgccgg cggcgccggg
     1621 gcggttgggc gcaggcagga cgatgccgag gcgcagcagg ttcagcttgc ggcggacgtc
     1681 ttccgggacc tgaacatcat tgaactgcgc cgcgccattg gccagttcga cctgcagctc
     1741 ggtgtattcg gcgttgatcc tgctctcgag ccacattgtg tcgtgggtga tgttggtggc
     1801 gcgcgtccac tggatgcggg ccgcctcctc gctgagggcg gcgaggcggg tttcggcatc
     1861 ggccacgaag gcgatggcgc tttcggcggt ggcgggcggc cgggccgagg tgggggccgg
     1921 aacgatctcc gcggcttcgc cggggttcat gccgaggccg gcgcaagccg cgctcccgag
     1981 cgccagggcg cagacggcgg tcgcggtcat cagggtgcgt ttcatgggct tcctcctcgc
     2041 gcggttcgcc cgcgtcgttc ggcggcagag gaggggagcg gggccgtgcc gtcaagcgtg
     2101 gctgtcagcc cagaacgcag gacgccagac cgtcgttgcg cactgtcccc atcgccgcct
     2161 ggatgcggga ggtgcggcgc cggacgtagg ggcccggact ggccgcgtcg tagcggcggg
     2221 gactgggcag gatggccgcg agacgggcgg cctcgcgggc ggtcagatcg tccgccgact
     2281 tgccgaacca gtggcgcgag gcggcctcgg ctccatagac gcccggggcc cattcggcga
     2341 cgttcagata gacctccatg atccgccgct tgccccagac ggtctcgatg gcgacggtgt
     2401 agccggcctc aagccctttg cggatccagt cgcgacccgg ccacaggaag gcgttcttgg
     2461 cggtctgctg gctgatggtc gagccgccgc ggatgcggtt gccgccccgc ttttccgagc
     2521 gggcgttggc cttgagcgcc ttctcgatgg ccttcatgtc gaagccccgg tgagcgcaga
     2581 aggtggagtc ctcggaagcg atcaccgc
//
LOCUS       DASVVN010000504         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_19030, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000504 DASVVN010000000
VERSION     DASVVN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19030"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VF686_08200"
     CDS             complement(<1..1568)
                     /locus_tag="VF686_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661835.1"
                     /translation="MGSLIRANAARAASKTPAAKVSTTKGHAGDVTVGERLRYAFDKS
                     MAAGPVALAGWLALVSLVTIALAATVLALTRIGPDGGFDFGEAAWQSLMRTMDAGTMG
                     GDSGWVFRFVTLFVTLMGILVFSALISIISAGLDSALEGLRKGRSRVLETDHTVILNW
                     SPSIFDILSELVIANASRRNPRIVIMADKDKVEMEDEIADKVPDLGNTRIICRNGDPT
                     DLFDLSLVNAQTCRSIIVDSPEGAADPDSQVIKTVLALTNDPQRRVERYRIAAELRSA
                     GNAEVARIVGGDEVQLVLADDLISRIVVQSSRQAGLSAVYSELLDFDGCEIYTTELEE
                     LVGVTFGDAVMGYEDSALIGLRYADGRVRMNPPMDEVITAGSRAIIIAEDDAAIRLAP
                     MAEDAVDPAAIRKPAPVKRTAERVLIIGWNRRGPMIAHELSRYVKAGSVLMIAGDVPG
                     LAEEVRALPLGSKNLKVEARIIDTSHAASLEALEPLGWDSIMVLGYSDHMEAQSADTR
                     TLITLLHLRKLSERL"
     gene            1809..>2890
                     /locus_tag="VF686_08205"
     CDS             1809..>2890
                     /locus_tag="VF686_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8661836.1"
                     /translation="MPATPARPEQDADALAISTFFDVSLDLLVIRELDGRVVRASPSW
                     ETVLGWRPDEMVGQPLLRLLHPDDQIATLESVIEVENRGPRDPMVDFKNRYRHKDGSY
                     RTIEWRARRVGDRVYGVARDVSDRVAAERELLDAKAAAEVANRAKTDFLANMSHEIRT
                     PLNGVIGLIGALGQGDLTPEQRELVALIEQSGVTLERLVSDILDVAKIEAGHLDFAIG
                     DMDLERELDGVIETARIKAEAKGLLFRVERGPGARGDFLGDATRIRQVLSNLLSNAVK
                     FTPEGQVSVAIEVADDTPDPGVAQLRLTVRDTGVGFDAAAGEALFQRFSQADSTITRR
                     FGGTGLGLSICRSLVEIMSGEITAEST"
BASE COUNT          467 a          982 c          989 g          452 t
ORIGIN      
        1 agccgctcgg acagtttgcg caggtgcagc agggtgatca gggtgcgcgt gtcggccgac
       61 tgggcctcca tgtgatccga atagcccagc accatgatgc tgtcccagcc cagcggctcc
      121 agcgcttcga gagaggcggc gtgactggtg tcgatgatcc gggcctcgac cttcaggttc
      181 ttcgagccca gcggcagggc gcggacttcc tcggcgaggc cgggaacgtc gccggcgatc
      241 atcagcaccg agcccgcctt cacatagcgc gacagctcgt gggcgatcat cgggccgcgg
      301 cggttccagc cgatgatcag cacccgctcg gcggtgcgtt tgaccggggc gggcttgcgg
      361 atggcggcgg ggtcgacggc gtcctcggcc atgggggcga ggcgaatggc ggcgtcgtcc
      421 tcggcgatga tgattgcgcg gctgcctgcg gtgatgacct cgtccatcgg cgggttcatc
      481 cgcacccggc cgtcggcata gcgcaggccg atcagggccg agtcctcata gcccatgacg
      541 gcgtcgccaa aggtgacccc gaccagctcc tccagctcgg tggtgtagat ttcgcagccg
      601 tcgaaatcga gcagttccga atagacggcg gacaggccgg cctgacggct ggactggacc
      661 acgatgcgcg agatcaggtc gtcggcgagg accagctgaa cctcgtcgcc gccgacgatg
      721 cgggcaacct cggcgttgcc ggcgctgcgc agttcggcgg caatgcgata gcgctcgacg
      781 cgccgctggg gatcgttggt cagggccagg acggtcttga tcacctggct gtcgggatcg
      841 gccgcgccct cgggcgaatc gacgatgatc gagcggcagg tctgggcgtt gacgaggctg
      901 agatcgaaca ggtcggtcgg gtcgccgttg cggcagatga tgcgggtgtt gccgaggtcg
      961 ggcaccttgt ccgcgatctc gtcctccatc tccaccttgt ccttgtcggc catgatgacg
     1021 atgcgcgggt tccggcggct ggcgttggcg atgaccagtt cggacaggat gtcgaagatc
     1081 gacggcgacc agttcaggat gacggtgtgg tccgtctcca gcacccgcga gcgccccttg
     1141 cgcaaaccct ccagcgccga gtcgagcccg gccgagatga tcgagatcag ggccgagaag
     1201 accagaatcc ccatcagggt cacgaacagg gtgacgaagc ggaagaccca gccggagtcc
     1261 ccgcccatgg tgccggcgtc catggtccgc atcagcgact gccaggccgc ctcgccgaag
     1321 tcgaagcccc cgtcggggcc gatgcgcgtc agggccagca ccgtcgccgc cagggcgatc
     1381 gttaccagcg acaccagcgc cagccagccc gccagcgcca ccggtcccgc tgccatgctc
     1441 ttgtcgaagg cgtagcggag gcgttcgccg acggtcacat cgccggcgtg acccttggtg
     1501 gtcgacactt tcgctgcggg cgtcttcgac gcggcgcgcg cggcgttggc gcggataagc
     1561 gagcccatga atactgtccc tcgccatcgc acggacggcg tcgtgatctc cgccgccccc
     1621 cacgaacggc gatcgcaccg gcggctgcag ccgcgcttcg ggacgaacgc cccttcctac
     1681 gacagaacaa cggtcgcgga tagtacaatc atgacgtgta tatatcgtct tggcacgagc
     1741 agagggccgt cttaacctcc gctggtgcag aatcaggcac gtccgcagtt ccgtcaggat
     1801 ttcgcttgat gccagccacg ccggcccgcc ccgaacagga cgccgacgcg ctggccatca
     1861 gcaccttctt cgacgtgtcg ctggaccttc tggtgatccg cgagctggac gggcgggtgg
     1921 tccgcgccag tccctcgtgg gagacggtgc tcggctggcg gcccgacgaa atggtgggcc
     1981 agccgctgct gcgcctgctg caccccgacg accagatcgc cacgctggaa agcgtcatcg
     2041 aggtcgagaa ccgcgggccg cgcgacccga tggttgattt caagaaccgc tatcgccaca
     2101 aggacggcag ctaccgcacc atcgaatggc gcgcccgccg ggtcggcgac cgcgtctatg
     2161 gcgtggcccg cgacgtcagc gaccgcgtcg ccgctgagcg cgaactgctg gacgccaagg
     2221 ccgcggccga ggtcgccaac cgcgccaaga ccgacttcct ggccaatatg agccacgaga
     2281 tccgcacccc cctgaacggt gtcatcggcc tgatcggcgc cttgggccaa ggcgatctga
     2341 cgccggagca gcgcgagctg gtggcgctga tcgaacagtc gggcgtgacg ctggagcggc
     2401 tggtttcgga cattctcgat gtcgccaaga tcgaggccgg ccatctggac ttcgccatcg
     2461 gggacatgga tctggagcgc gagctggacg gcgtcatcga gaccgcgcgg atcaaggccg
     2521 aggccaaggg cctgctgttc cgcgtcgaac gcggcccggg cgcgcgcggc gacttcctcg
     2581 gcgacgccac ccgcatccgt caggtgctgt ccaacctgct gtccaacgcc gtcaagttca
     2641 cgcccgaggg gcaggtctcg gtcgccatcg aggtcgccga cgatacgccg gacccgggcg
     2701 tcgcccagct gcgcctgacc gtgcgcgaca ccggcgtggg cttcgacgcc gccgccggcg
     2761 aggccctgtt ccagcgcttc agccaggccg acagcaccat cacccgccgc ttcggcggca
     2821 ccggcctggg cctgtccatc tgccgctcgc tggtcgagat catgagcggc gagatcacgg
     2881 ccgaatccac
//
LOCUS       DASVVN010000505         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_466245,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000505 DASVVN010000000
VERSION     DASVVN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466245"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VF686_08210"
     CDS             complement(<1..1064)
                     /locus_tag="VF686_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX8661837.1"
                     /translation="MTIPFIDLQAQRLRLNGRIEAAVQEAVVGGAWVMGPQVRQFEAD
                     LAAFGQAKHALGCANGTDALALPLMAWGIGRGDAVFVPSFTFAATAEIVPWFDAEPVF
                     IDVDAKTYNMDPGHLEAAIEATIKEGRLKPRVVIAVDLFGQPADYPAIKAICDKHGLK
                     LICDSAQGFGCTIEGEHPLKWADITTTSFFPAKPLGCYGDGGAVLTNDDDLAQLIDSI
                     RVHGKAVARDLEGKTFDHDPKYLNMRIGLNSRLDTIQAAVLIEKLKVFGQEIQWRNRI
                     AARYNEGLRPHVAAVPDVPAGNVSNWAQYTIEHEDRDGLAAHLKELGVPTAVYYPIPL
                     HLQPAYDIYPRGPQGLPVTER"
     gene            1232..1744
                     /locus_tag="VF686_08215"
     CDS             1232..1744
                     /locus_tag="VF686_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8661838.1"
                     /translation="MSTGVPAVFLDRDGVLIEDTGYPHLEEHLRLVPGAPEAVRRLNQ
                     LGYLCVIVTNQSGVARGLFTEDQMKAFNALIVRRLAARGAIIGAVYACPFHSEGRVEA
                     YIHPDHPDRKPNPGMILRAIADHQIDPARSFMIGDQPSDMEAARRAGVPGFRFEGGDL
                     DAFVRDLLGH"
BASE COUNT          283 a          631 c          621 g          310 t
ORIGIN      
        1 cgctcggtga ccggcaggcc ctgcggcccg cgcggataga tgtcataggc cggctgcagg
       61 tgcagcggga tcggataata gaccgccgtc ggcaccccca gttccttcag atgcgcggcc
      121 agaccgtccc ggtcctcgtg ctcgatggtg tactgggccc agttggagac gttgccggcc
      181 ggcacgtccg gcacggcggc gacgtgcggg cgcaagccct cgttgtagcg ggccgcgatc
      241 cggttgcgcc attggatctc ctgaccgaac accttcagct tctcgatcag gacggcggcc
      301 tggatggtgt ccagacggct gttcaggccg atgcgcatgt tcagatattt ggggtcgtga
      361 tcgaaggtct tgccttccag atcgcgcgcc accgccttgc cgtgcacgcg gatggaatcg
      421 atcagctggg ccaggtcgtc gtcattggtc agcaccgccc cgccgtcgcc gtagcagccc
      481 agcggcttgg ccgggaagaa gctggtggtg gtgatgtcgg cccatttcag cggatgctcg
      541 ccttcgatgg tgcagccgaa gccctgggcc gagtcgcaga tcagcttgag gccgtgtttg
      601 tcgcagatcg ccttgatggc cggatagtcg gcgggctggc cgaacaggtc gacggcgatg
      661 accacgcgcg gcttcagccg gccttccttg atcgtcgcct caatcgcggc ctcgagatgg
      721 cccgggtcca tgttgtaggt cttcgcatcc acgtcgatga agaccggctc ggcgtcgaac
      781 cacggcacga tctcggcggt ggccgcgaag gtgaagctgg gcacgaagac cgcgtcgccg
      841 cgcccgatcc cccacgccat cagcggcagg gccagggcgt ccgtgccgtt ggcgcagccg
      901 agggcgtgct tcgcctgacc gaaggcggcc agatcggcct cgaactggcg cacctgcggc
      961 cccatcaccc aggcgccgcc gaccacggcc tcctgcacgg cggcctcgat ccggccgttc
     1021 agacgaagac gctgggcttg caggtcgatg aagggaatgg tcatgggggt ctccggtcgg
     1081 tccgggccgc agacgcggcg ggacggcgct ggccgatgcc gcgccggggt ggggcctcct
     1141 accaaacgag gggccgtcaa gccaaaccca taagggtcac ttcccgtgac gggttgcaac
     1201 gacggcgtac ggcagcgtat gcgacccgat catgagcacc ggcgttccgg ccgtgtttct
     1261 cgatcgcgac ggcgtcctga tcgaggacac tggctatccc catctggaag agcatctgcg
     1321 cctcgtgccc ggcgcgcccg aggccgtgcg ccggctgaac cagctcggct atctgtgcgt
     1381 catcgtcacc aaccagtcgg gggtggcgcg cggcctgttc acagaagacc agatgaaggc
     1441 gttcaatgcc ctgatcgtgc gccgcctggc cgccaggggc gcgatcatcg gcgccgtcta
     1501 cgcctgcccc ttccattccg agggccgcgt cgaggcctat atccaccccg accatcccga
     1561 ccggaagccg aaccccggca tgatcctgcg cgccatcgcc gatcaccaga tcgacccggc
     1621 cagaagtttc atgatcggcg accagccttc cgacatggag gccgcccgcc gcgccggtgt
     1681 gcccggcttc aggttcgagg gcggcgacct cgacgccttc gtccgggacc tgctcggcca
     1741 ttagggcgac ggcccgatgt gggatttccc gctccgatga aataggctgc ggttcgaccg
     1801 cccggagcct cgcatgtccc agaccttcgc cgaccgccgc tggac
//
LOCUS       DASVVN010000506         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_414536,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000506 DASVVN010000000
VERSION     DASVVN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414536"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3640
                     /locus_tag="VF686_08220"
     CDS             <1..>3640
                     /locus_tag="VF686_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8661839.1"
                     /translation="DFTGNSVSGVGASGFNPAGEAPYGAVLMTRGVNQLNIVGNTFVD
                     NNQVLAIEPGRGLSTNTFLAGNSFINNRYGIYMPGNALTGGAVTFGAGNSFVAGADTV
                     QHIVWRGAAGLDLTNVLFDGKLTSGMSLAELFDLEDLVSHATDVAGAGFATFKAGEIF
                     VTTGSGSVQRGIDVAAAGNTVHVDAGTYALPGELSITKSLSLIGAGAGGTIFNSASTG
                     YGVFATADNITLSGFTFNGNPTGSYGVKVQPNSADPAARVHGFTITDVAINGTRRTGL
                     DLNGVVGAVIDGVTVTGAVAGNGISITDSADVVVRNTTTSGNAWGGLALYQTNRFFDQ
                     QVGDIVVEASNVFGEVRGLYTQDESALFDFGALTLLGFDFTVRNDVFQPDAAGYTFYK
                     TTLQGALDYGVALGAAGSYVQGWNGSQGDNHFYVGGGDAGAGQQFLSIQTAFDKSSAG
                     SVIDIFSGAYTGVATLTGQRDLRFGVVTVGGLNLTSGASGTIISGDITATGGLDFDGT
                     IVLGGDTHLAADGDISLIGVDGQPGGQQSLAIEGQGAVYVGQVGASVALASLSVVGAD
                     VTARGATTTGDQFFSGDTVLLWGGFDAGGDFTVDGPIILGGDSVIDSDGDILLGSIDG
                     EHNLSINAGGAVDLGVIGAAEALNGLNVLGAAITSRGATTQAFQSWNAATVGLSGAYA
                     AGGDITVNGAAKLNGSTSLTSDDYVALGSVNGAHDLTIKAFNFASLGPVGGTTALVDL
                     VVEAGQITAVGARTTGSQAFSGARVGLSGAFIAGGGFAVDGAMDLNGDVAIDGDDVAL
                     GSVDGSHDLTIDARGAVAFGAVGSTTALASLDISGASIASHGAITSGDQTYDAATVGL
                     SGAYAAGGDLVVDGAALLTGAASLDADGTVVLGTVDGAHSLSIGAGGSASLGAIGGTA
                     ALANLDVTAAAIASNGAVTTGDQSWNGATVGLSGLYAAGGDFVVDGAANLNGATSIDA
                     DAVVTGTIDGARHLSIAANGAVSLGVIGATTALTGLDVSGASIVARGARTVGAQSWTG
                     QAIRLAGGFVIDNGDFSLIGLTTLDGATTIATNGGDVVAGTVNGSSAGGQSLSIDAGA
                     GTASVGSMGAGVRLGAMLVRSAGAVLNGSTYAGSSLTFLGSGAGSTVRLTQAVTTFNS
                     APIGSTAGHILVQPDLIGTVNGAQSVVFNAGTGVGANNGDITLGNAGSEALRLGAMTV
                     SGDDFSAQT"
BASE COUNT          540 a         1317 c         1231 g          552 t
ORIGIN      
        1 cgacttcacg ggcaacagcg tcagcggcgt cggggccagc ggcttcaatc cggccggcga
       61 agcgccctac ggggctgtgc tgatgacgcg cggcgtcaac cagctgaaca tcgtcggcaa
      121 caccttcgtc gacaacaacc aggtgctggc gatcgagcca ggccgcggtc tgtcgaccaa
      181 caccttcctg gccggcaaca gcttcatcaa caaccgctat ggcatctaca tgcccggcaa
      241 cgccctgacc ggcggcgcgg tgacgttcgg ggcgggcaac agcttcgtcg ccggggcgga
      301 cacggtgcag cacatcgtct ggcgcggcgc ggcgggcctg gacctgacca atgtcctgtt
      361 cgacggcaag ctgacctcgg gcatgagtct ggccgagctg ttcgacctcg aggacctggt
      421 cagccacgcg accgacgtcg ccggcgccgg cttcgccacc ttcaaggccg gcgagatctt
      481 tgtcaccacc ggttccggtt cggtccagcg cggcatcgat gtcgcggcgg cgggcaacac
      541 cgtccacgtc gacgcgggga cctatgcgct tcccggcgaa ctgagcatca ccaagagcct
      601 cagcctgatc ggcgcgggag ccggcggcac catcttcaac tccgcctcga ccggctacgg
      661 cgtcttcgcg acggccgaca acatcacgct gagcggcttc accttcaacg gcaacccgac
      721 cggcagctat ggcgtgaagg tccagccgaa ctcggccgat ccggccgcgc gcgttcacgg
      781 cttcaccatc accgacgtgg cgatcaacgg aacgcgccgc acgggtctcg acctcaacgg
      841 ggtcgtggga gcggtcatcg acggcgtcac cgtgaccggc gccgtcgccg gcaacggcat
      901 ctcgatcacc gactcggccg acgtcgtggt gcgcaacacc accacctccg gcaacgcctg
      961 gggcgggctg gcgctctatc agaccaaccg gttcttcgat cagcaggtcg gcgacatcgt
     1021 cgtcgaggcc agcaacgtct tcggggaggt ccgcggcctc tacacccagg acgaatcggc
     1081 cctgttcgac ttcggcgctc tgaccctttt gggcttcgac ttcaccgtcc gcaacgacgt
     1141 cttccagccg gacgccgccg gatacacctt ctacaagacc accctccagg gcgcgctgga
     1201 ctacggcgtc gcccttggcg cggcgggcag ctacgtccag ggttggaacg gcagccaggg
     1261 cgacaaccac ttctacgtgg gcggcggcga cgccggtgcg ggccagcagt tcctgtcgat
     1321 ccagaccgcc ttcgacaagt cgtcggccgg atcggtgatc gacatcttct cgggcgccta
     1381 tacgggcgtc gccaccctga ccggccagcg cgacctccgc ttcggcgtgg tcacggtcgg
     1441 cgggctcaac ctgacctcgg gcgcgtccgg aacgatcatc tcgggcgaca tcaccgccac
     1501 gggcggcctc gacttcgacg gtacgatcgt gctgggcggc gatacgcatc tggcggcgga
     1561 cggcgacatc agcctgatcg gcgtcgacgg ccagcccggc ggccagcagt cgctggcgat
     1621 cgagggccag ggcgcggtct atgtcggtca ggtcggcgcc tcggtcgcct tggcctcgct
     1681 ctcggtcgtc ggggccgacg tcaccgcgcg cggcgcgacc accacgggtg accagttctt
     1741 ctccggagac accgtcctgc tgtggggcgg cttcgacgcc gggggcgact tcaccgtcga
     1801 cggcccgatc atccttggcg gcgacagtgt catcgacagc gacggcgaca tcctgctcgg
     1861 ctccatcgac ggcgagcaca acctgtcgat caacgccggc ggcgcggtgg atctcggcgt
     1921 gatcggcgca gccgaggccc tgaacggcct taatgttctg ggtgcggcca tcacctcgcg
     1981 cggcgcgacc acccaggcct tccagagctg gaacgcggcc acggtcggac tgtccggcgc
     2041 ctatgccgcg ggcggcgaca tcaccgtcaa cggggcggcc aagctgaacg gctcgaccag
     2101 cctgaccagc gacgactatg tcgccttggg ctcggtcaac ggcgcccacg acctgacgat
     2161 caaggccttc aacttcgcca gcctgggtcc cgtcggcggg actacggcct tggtcgatct
     2221 ggtcgtcgag gccgggcaga tcaccgccgt cggcgcccgg accacgggca gtcaggcctt
     2281 ctccggcgcc cgcgtcggac tgtccggcgc cttcatcgcc ggcggcggct tcgcggtcga
     2341 cggtgcgatg gacctgaacg gcgacgtcgc catcgacggc gacgatgtcg cgctcggttc
     2401 ggtggacggc tcccacgacc tgaccatcga cgcccgcggc gcggtcgcct tcggagcggt
     2461 cggttccacc acggccctgg cttcgctgga catctccggc gcctccatcg cctcgcatgg
     2521 cgcgatcacc agcggcgacc agacgtatga cgcggccacc gtcggcctgt cgggggcgta
     2581 tgccgcaggc ggggatctgg tcgtcgacgg tgcggctctg ctgaccggcg cggccagcct
     2641 ggatgcggac gggaccgtcg tcctcggtac ggtcgacggc gcgcattcgc tgtcgatcgg
     2701 cgcgggcggc tcggccagcc tgggtgcgat cggcgggacc gccgccttgg ccaatctgga
     2761 cgtgaccgcg gccgccatcg cctcgaacgg cgctgtcacc accggcgacc aatcctggaa
     2821 cggggccacg gtgggcctgt ccggcctcta tgccgccggc ggcgacttcg tggtcgatgg
     2881 cgcggcgaac ctgaacggcg cgacctcgat tgacgccgac gccgtggtga cgggaaccat
     2941 cgacggcgcc cggcatctgt cgatcgccgc caacggcgcg gtcagcctcg gcgtcatcgg
     3001 cgcgaccacc gcattgaccg gcctggatgt ctcgggggcc tcgatcgtcg cccgcggcgc
     3061 ccgcaccgtc ggcgcgcaat cctggaccgg gcaggcgatc cgcctggccg gaggcttcgt
     3121 gatcgacaac ggggacttca gtctgatcgg tctgaccacc ctggacggtg cgaccaccat
     3181 cgccaccaac ggcggcgacg tcgtcgcggg gaccgtcaac gggtccagcg ccggtggcca
     3241 gtcgctgtcg atcgacgccg gcgcgggcac ggcctcggtc ggttcgatgg gggcgggcgt
     3301 gcgtctcggc gccatgctgg tccgctcggc cggcgccgtg ctgaacggct cgacctacgc
     3361 cggatccagc ctgacgttcc tcggctcggg cgccggatcg accgttcgcc tgacccaggc
     3421 ggtgacgacc ttcaacagcg cgcccatcgg ttcgaccgcc ggccacatcc tcgttcagcc
     3481 cgacctgatc ggcacggtca acggagccca gagcgtggtg ttcaacgccg gcacgggcgt
     3541 aggcgccaac aacggggaca tcaccctcgg caacgcgggc agcgaggcgc tgcgcctcgg
     3601 cgccatgacg gtcagcggcg acgacttctc cgcccagacc
//
LOCUS       DASVVN010000507         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_390265,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000507 DASVVN010000000
VERSION     DASVVN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390265"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1914
                     /gene="metG"
                     /locus_tag="VF686_08225"
     CDS             205..1914
                     /gene="metG"
                     /locus_tag="VF686_08225"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8661840.1"
                     /translation="MSRILITSALPYINGIKHLGNLAGSMLPADVYARFKRAQGHEVL
                     YICATDEHGTPAELAAAAAGQDVRTYCDEQHEVQKAAGQAFGLSYDWFGRSSNAPNQR
                     LTQHFAHVLEQNGLIEERVDRMIYSVDDARFLPDRYVEGTCPHCAHVGARGDQCDNCG
                     RLLDPTDLIDPYSAVSGSRNLEVRDTRHLYLLQTKLADRIRAWIGSKGDWQTLARSIA
                     LKHLDEGLIDRGITRDLKWGVPVVGPDGGPRPGMEGKVFYVWFDAPIEYIGATEEWAD
                     ATGRTWRDWWRTDEGAEDVRYVQFMGKDNVAFHTVSFPATILGSGEPWKTVDTLKAFN
                     WLNWYGGKFSTSQKRGVFMDQALELLPADYWRWHLTAYGPEHSDAAFTWEQFQSTTNK
                     DLADVLGNFVNRIVKFAESKFDGVVPDAGEPGPLEAKLEADLRAGIAEATAQFEAMEF
                     RKAAVALRAVWVLGNEYLQEAAPWTAFKTDVERSAVGVRTGLNLVALFARLAAPVMPD
                     SAVKIAAAVGATDLSWPAADAPLLDALPRGQTVAATGVLFTKIEDAQVAEWSERFGGA
                     EAA"
     gene            1911..>2272
                     /locus_tag="VF686_08230"
     CDS             1911..>2272
                     /locus_tag="VF686_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661841.1"
                     /translation="MTDARSAAFAGLLAGDQAEDRAVLAGTSAYVALRSRDRARREAV
                     VALLEDGWPEDSDDLYAAAWVLNHGDEAQEAALGSQLAARAAELGHPKARWLAAAAFD
                     RSLMYAGKPQKYGTNMVP"
BASE COUNT          357 a          753 c          805 g          357 t
ORIGIN      
        1 ggtgatcatc gccagtctgg tgtcgtcggt cctgctgacc gggcggttcg tgcgggtggc
       61 gcggcggctg ccgcagggat gagggatgag ggatgtgtca ctaggcggcg gcttgcgatt
      121 tggcgtcgca ctcgccacta accgtgacca gtccctactc cctcatccta gtccctcatc
      181 cctagtccct catccccggc cttcatgtcc cgcatcctga tcacctcggc cctgccgtac
      241 atcaacggca tcaagcatct ggggaatctg gcgggctcga tgttgccggc ggatgtctac
      301 gcccggttca agcgggcgca ggggcatgag gtcctgtaca tctgcgccac cgacgaacac
      361 ggcaccccgg ccgagctggc cgccgccgcc gccggtcagg acgtgcggac ctattgcgac
      421 gaacagcacg aggtccagaa ggcggccggg caggcgttcg gcctcagcta tgactggttc
      481 ggccggtcct cgaacgcccc caaccagcgc ctgacgcagc atttcgccca tgtgctggag
      541 cagaacggcc tgatcgagga gcgcgtcgac cggatgatct attccgtcga cgacgcccgc
      601 ttcctgcccg accgctatgt cgaggggacg tgtccgcact gcgcccatgt cggcgcgcgc
      661 ggggaccagt gcgacaactg cgggcggctg ctggacccga cggacctgat cgatccgtat
      721 tcggcggttt cgggctcgcg caatctcgag gtccgcgaca cccgtcacct gtatctgctg
      781 cagacgaagc tggccgaccg gatccgcgcc tggatcgggt cgaaaggcga ctggcagacg
      841 ctggcccgat ccatcgccct gaaacatctc gacgaaggcc tgatcgaccg cggcatcacg
      901 cgcgacctga aatggggcgt gccggtagtc ggtcccgacg gcgggccgcg gccgggcatg
      961 gagggcaagg tcttttacgt ctggttcgac gccccgatcg aatacatcgg cgcgaccgag
     1021 gaatgggccg atgcgacggg acggacctgg cgtgactggt ggcggacgga cgagggcgcc
     1081 gaggacgttc gctacgtcca gttcatgggc aaggacaacg tcgccttcca caccgtcagc
     1141 ttcccggcga ccatcctcgg ctcgggcgag ccgtggaaga cggtggacac gctgaaggcc
     1201 ttcaactggc tgaactggta tggcgggaaa ttctcgacca gccagaagcg cggcgtcttc
     1261 atggatcagg cgctggagct gctgcccgcg gactactggc gctggcacct gacggcctat
     1321 gggcccgagc attccgacgc cgccttcacc tgggagcagt tccagtcgac gaccaacaag
     1381 gacctggccg acgtgctggg caatttcgtc aaccgcatcg tcaaattcgc agagagcaag
     1441 ttcgacggcg tggtgccgga cgcgggcgag cccggtcctc tggaggcgaa gctggaggcc
     1501 gacctgcgcg ccggcatcgc cgaggccacc gcccagttcg aggccatgga gttccgcaag
     1561 gccgccgtcg ccctgcgcgc ggtctgggtg ctgggcaatg aatacctgca ggaggcggcg
     1621 ccctggacgg ccttcaagac cgacgtcgaa cggtcggcgg tgggcgtgcg cacgggtctg
     1681 aacctcgtcg ccctgttcgc ccggctggcc gcgcccgtca tgccggactc ggcggtgaag
     1741 atcgcggcgg ctgtgggagc gacggacctc agctggcccg cggccgacgc gcccctgctg
     1801 gacgccctgc cgcgcggtca gaccgtggcc gcgaccgggg tccttttcac caagatcgag
     1861 gacgctcagg tggccgaatg gtccgagcgg ttcggcggcg ccgaggccgc gtgactgacg
     1921 cccggtccgc cgccttcgca gggctgctgg ccggggatca ggccgaggac cgggccgtgc
     1981 tggccggaac gtcggcctat gtcgccctga gatcgcgcga ccgggcgcgg cgtgaggcgg
     2041 tcgtggcgct gctggaggat ggctggcccg aggacagcga cgatctgtac gccgccgcct
     2101 gggtcttgaa tcacggggac gaggcccagg aggccgcgct gggcagccag ctggcggcgc
     2161 gggcggcgga acttggccat ccgaaggcgc ggtggctggc cgccgcggcg tttgaccggt
     2221 cgctgatgta cgccggcaag ccgcagaaat acgggaccaa catggtcccc ga
//
LOCUS       DASVVN010000508         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_19360, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000508 DASVVN010000000
VERSION     DASVVN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19360"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VF686_08235"
     CDS             <1..672
                     /locus_tag="VF686_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8661842.1"
                     /translation="PRIAADLDARGWALTGPLLTPQTCIALQGLYADPDRFRSRVVMG
                     RHGFGEGEYQYFANPLPDPLPALRRRLYAGLAPIANGWNARLGRPADFPADLDAFAAR
                     CHAAGQTRPTSLLLKYGPGDYNRLHQDLYGELVFPLQTAVLLSDQVRDFDGGELVLVE
                     QKPRSQSQAHVVPLQQGHGVVFAVRERPAAGVRGDYRVQMRHGVSRVRSGARMTLGLI
                     FHDAA"
     gene            complement(675..1022)
                     /locus_tag="VF686_08240"
     CDS             complement(675..1022)
                     /locus_tag="VF686_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8661843.1"
                     /translation="MQGIWKLAAVAACGLTLTGCATTDNYASACQRDYERNRQYATAS
                     GAALGAAVGAAIAGENDRERGAAIGALAGGLLGNQLSARDDPCGYGFGGYNTDGRYGY
                     TRVPRRGDYGYYP"
     gene            1850..3103
                     /locus_tag="VF686_08245"
     CDS             1850..3103
                     /locus_tag="VF686_08245"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF005536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX8661844.1"
                     /translation="MTNRPSHIRLNSHPGAGTVSRFPLNWGAPTAKERGPVIGTVNSG
                     SDRNAIGAHGGAYSVYRALAVSSGAMSANNRPDLTNTSPTTPMGPFPQWTDASKIVSL
                     DPWGARVAEDFANEIRDGVDIRPTIAVTRARLQLGEIGEAIAAGRLQADGQIVHDSGD
                     VAVTKAAIDPVWYLPGIAERFGVTETALRRTLFEQTGGMYADLVTRPDIKVFLPPIGG
                     ITLYIFGDPAAIPDPKRRLTCRVHDECNGSDVFGSDICTCRPYLTHGIEECIREAQDG
                     GAGLIAYNRKEGRALGEVTKFLVYNARKRQPGGDQAATYFERTECVAGVQDARFQQLM
                     PDILHWLGITKIDRFVSMSNMKHDALTASGIEIGERVPIPDYLVPPDASVEMEAKKAA
                     GYFTPDAPPSAEDLTRVVGRDLEQF"
     gene            3090..>3902
                     /locus_tag="VF686_08250"
     CDS             3090..>3902
                     /locus_tag="VF686_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HEX8661845.1"
                     /translation="MSSSETQQAHSLLNAAAVRERAHEMLEIGVAGGLDHWWVDLERL
                     PEVARYVAAVIRDQYPTLAVPFHARWRHFAIDGVDLWGPTAEDRDWLDPASRARAEFD
                     LAIVSVLLDAGAGPGWSWRDAQTGRTLTRSEGLAIASLRMFEAGAFSVTEDDPWRVDA
                     VALTLLTADTLAKGFQVTADNPLVGLDGRAALLRRLGQACLAEPDLFALEDEPRPGGL
                     FDAMVDRADDQDRLPAPVILEVLLEALGPVWRDRLTLGGVDLGDCWTHPALKR"
BASE COUNT          597 a         1504 c         1230 g          571 t
ORIGIN      
        1 ccccgcatcg ccgccgatct cgacgcccgg ggctgggccc tgaccggccc gctgctgacg
       61 ccgcagacct gcatcgccct gcaaggcctc tatgcggacc ccgaccgctt ccgcagccgc
      121 gtggtcatgg gccgtcacgg cttcggcgag ggcgaatacc agtatttcgc caatcccctg
      181 cccgatccgc ttccggccct gcgtcgccgc ctctatgcgg gcctcgcgcc gatcgccaac
      241 ggctggaacg cccgcctcgg ccgtcccgcc gactttcccg ccgatctgga cgccttcgcc
      301 gcccgctgcc acgccgccgg ccagactcgc ccgacctccc tgctgctgaa atacgggccc
      361 ggcgactaca accggctgca tcaggacctg tacggcgagc tggtcttccc gctccagacc
      421 gccgtcctgc tcagcgatca ggtccgggac ttcgacggcg gcgaactggt gctggtcgaa
      481 cagaagcccc gcagccagtc acaggcccac gtcgttccgc tgcaacaggg ccatggcgtc
      541 gtcttcgccg tccgcgaacg gcccgccgca ggcgtgcgcg gcgactatcg cgtgcagatg
      601 cgccacggcg tcagccgcgt ccgctcaggc gcccggatga cgctgggcct gatcttccac
      661 gacgcggcct gacctcaggg atagtagccg taatccccgc gccgcggcac gcgcgtatag
      721 ccgtaccggc cgtcggtgtt gtagccgccg aagccatagc cgcacggatc gtcgcgcgcc
      781 gaaagctggt tgcccagcag gccgccggcg agggcgccga tggcggctcc gcgctcgcgg
      841 tcgttctcgc ccgcgatcgc cgctccgacc gccgcgccca gcgccgcgcc ggacgccgtc
      901 gcatactgcc ggttccgctc ataatcgcgc tgacaggccg acgcatagtt gtcggtcgtg
      961 gcgcagcccg tcagcgtcaa tccgcaggcc gcaaccgccg ccagtttcca gatgccttgc
     1021 atgccgtctc tcctcgcagc cccgtgctcc aacggcgaag ggggagggtg cgttccgggg
     1081 cgggcctccg tctcccatcg gagagggctt gagcgcactc tcacctccgt gcatccccgc
     1141 gaaggcgggg acccaggttt gagcggcagc cggactgtct gaggcaaccg gcgctcgttc
     1201 agacacccgc gcgaggccaa agctcgggtc cccgccttcg cggggatgca cggtgttttt
     1261 cgggccagga ataaacttcc agccatacag attgcaaagc ccaacccgct gatcccgctg
     1321 cgctaatcgg accgaccagc gtcatttcgt agccttggtc gggaccggcc ccataatccc
     1381 tgcatcccgt cggtggggag ggcgtcggat ccacgttcct cgatgtcggc gggctgtggt
     1441 cgagcgatga agcccggggg cgcttacgcc gggggtcctt gaggacgtcg atggatggcg
     1501 tcttcctgct cgccgcgcgc ttcttgtgcg ggacctctgc ggactatacg cccgcaagtc
     1561 tccccccgcc cggctccgca aggagacgga ccgggtgtcg gcaaggccag acaacggccg
     1621 gcggccggca cgcgcgcgga aaacgaccac gcggggcgac tgtctcgtcg aaacggtaaa
     1681 actacaacaa gcaccgggcg agcccggtcg ccccgcgccc tccccgaact tttccgcgga
     1741 aacgccgaaa gggcgatgac gcatgcccac tggacagaac ccgcgaccgg gtgtgtcctg
     1801 cagagtccgg cgcaagaaaa acaacaagcc ggttcgagtg cgaggaaaca tgaccaaccg
     1861 accgagccac atccggctca actcccaccc cggcgcgggg accgtcagcc gctttcccct
     1921 gaactggggt gcgcccaccg caaaggagcg cggccccgtc atcggcaccg tcaactcggg
     1981 cagcgaccgc aacgccatcg gcgcccacgg cggggcctat tcggtctacc gggcgctcgc
     2041 cgtctcctct ggcgcgatga gcgccaacaa ccggcccgac ctgaccaaca cctcgccgac
     2101 cacgcccatg ggcccgttcc cgcagtggac ggacgcctca aagatcgtct cgctggaccc
     2161 ctggggcgcc cgggtggccg aggacttcgc caacgagatc cgcgacggcg tcgacatccg
     2221 ccccaccatc gccgttaccc gcgcccgcct tcagctgggc gagatcggcg aggctatcgc
     2281 cgcgggccgg ctccaggccg acggccagat cgtgcatgac agcggcgacg tcgccgtcac
     2341 caaggccgcc atcgatccgg tctggtatct gcccggcatc gccgaacgct ttggcgtcac
     2401 cgagaccgcc ctacggcgca ccctgttcga acagacgggc ggcatgtacg ccgacctcgt
     2461 cacccggccc gacatcaagg tcttcctgcc ccccatcggc ggcatcaccc tctacatctt
     2521 cggcgacccg gccgcgatcc ccgacccgaa gcgccgcctg acctgccggg tccatgacga
     2581 gtgcaatggc tcggacgtgt tcggctccga catctgcacc tgccgcccct acctcaccca
     2641 cggcatcgag gaatgcatcc gcgaggccca ggacggcggc gcgggcctga tcgcctataa
     2701 ccgcaaggag ggccgggccc tcggggaagt cacaaaattc ctcgtctaca acgcccgcaa
     2761 gcgccagccg ggcggcgacc aggctgccac ctatttcgag cgcaccgaat gcgtcgccgg
     2821 ggtgcaggac gcccgcttcc agcagttgat gcccgacatc ctgcactggc tggggatcac
     2881 gaagatcgac cgcttcgtct ccatgtcgaa catgaagcac gacgccctga ccgcctcggg
     2941 catcgagatc ggcgagcggg tgcccatacc cgactatctg gtgccgcccg acgcctcggt
     3001 ggagatggag gccaagaagg ccgccggcta tttcaccccc gacgcgccgc cgtcggccga
     3061 ggacctgacc cgcgtggtgg gccgcgacct tgagcagttc tgagacgcag caggcgcaca
     3121 gcctgctcaa cgccgccgcc gtccgcgaac gggcccatga gatgctcgag atcggcgtcg
     3181 ccggcggcct cgaccactgg tgggtcgacc tcgaacgcct gccggaagtc gcccgctatg
     3241 tcgccgcggt gatccgggac cagtatccga cgctggccgt gcccttccac gcccgctggc
     3301 ggcatttcgc catcgacggc gtcgacctgt ggggcccgac cgcggaagac cgcgactggc
     3361 tcgaccccgc ctcccgcgcc cgggccgagt tcgacctcgc catcgtctcg gtcctgctcg
     3421 acgccggcgc cggccccggc tggtcctgga gggatgcgca gaccggccgc accctgacgc
     3481 gctccgaggg tctggccatc gccagcctgc gcatgttcga ggccggcgcc ttctcggtca
     3541 ccgaggacga tccgtggcgc gtcgacgccg tggccctgac cctcctgacc gccgacaccc
     3601 tggccaaggg cttccaggtc accgccgaca atccattggt cggtctcgac ggccgcgccg
     3661 ccctgctgcg ccgtctcggt caggcctgcc tcgccgagcc cgacctgttc gcgctggagg
     3721 acgagccccg ccccggcggc ctgttcgacg ccatggtcga ccgcgccgac gatcaggacc
     3781 gcctccccgc ccccgtcatc ctcgaagtcc tgctggaggc gctcggcccg gtctggcgcg
     3841 accgcctcac cctgggcggc gtcgacctcg gcgattgctg gacgcacccg gcgctgaaac
     3901 gc
//
LOCUS       DASVVN010000509         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_72062, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000509 DASVVN010000000
VERSION     DASVVN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72062"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="mreC"
                     /locus_tag="VF686_08255"
     CDS             complement(<1..654)
                     /gene="mreC"
                     /locus_tag="VF686_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8661846.1"
                     /translation="MAFRDGPFENIKVPLVWTAAVAVVLAAIGCILLLISDARAQTSP
                     YQPVRAGVESVAGPVGGVFAAPVRWFGSATDYVGGYFFAVSENRRLKRELAELAPWRD
                     QAIALKNVNARYEAMLGIRPEPPVAMQTARSISDTRGPFSRARLIDAGSAKGVKVGNP
                     VINEHGLVGRIVGVSSNVSRMVLLTDVASRTPVLVDRTDARALLTGDGSDNPRLEFIR
                     "
     gene            complement(792..1832)
                     /locus_tag="VF686_08260"
     CDS             complement(792..1832)
                     /locus_tag="VF686_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8661847.1"
                     /translation="MFSSIFGAISNDIAIDLGTANTLIYMKGKGIVLNEPSVVALRNV
                     GGRKIVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKHFIRKVHNRKGF
                     VNPKVIVCVPSGATAVERRAINDSCLNASARRVGLIDEPMAAAIGAGLPIHEPTGSMV
                     VDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRNHNLLIGETTAERIKKD
                     IGTARIPADGEGLSIDVKGRDLMQGVPREVRISERQAAEALAEPVTQIVEAVKVALEA
                     TPPELAADIADKGIMLTGGGALLRGLDAEIRDHTGLPVSVADDPLSCVAIGCGRVLEH
                     PRWMKGVLDSAL"
     gene            complement(2032..2748)
                     /locus_tag="VF686_08265"
     CDS             complement(2032..2748)
                     /locus_tag="VF686_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8661848.1"
                     /translation="MRRVLDFILNMEVRRWRAVLATALLLGATLGLLAIGKSQFGLAA
                     EENLEAWLQGYRGSPWAFAATVVLFIAAAFIGAPQFILIAACVVAFGPSMGFFYSWAA
                     TIVSAGVTYWLGRGPTARLVDRFGGRTMGRLKGFVGKNAFYASFMIRNVPSAPFIVVN
                     MAFGAARASFWRFLAGCALGVLPKTALVSFFGGAVVSAVSGDGVWTSLILAGVAVVWL
                     GLMLGVRELVKRREAASGAD"
     gene            complement(2755..>3216)
                     /locus_tag="VF686_08270"
     CDS             complement(2755..>3216)
                     /locus_tag="VF686_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661849.1"
                     /translation="NDVAKLESCLTLEGDLVAAATLMNEKGRVTAFGVQEINETDWSL
                     WVGHANQNARLDWNGEIIQETNSFGPGDPMPRTISEFMSGQANRLGGIHVEVRTVGAL
                     SSSSAWIEGAVIHYRDRKTYRAASRQEWVRPDGFDWALKSWTVGPFAPVAD"
BASE COUNT          545 a         1061 c         1100 g          510 t
ORIGIN      
        1 gcggataaac tccagccgcg gattgtcgct gccgtcgccg gtgagcaggg cgcgggcgtc
       61 ggtgcggtcg accagaacgg gcgttcggct ggcgacgtcg gtcagcagca ccatccggct
      121 gacgttcgac gaaacgccga cgatgcggcc gaccagtccg tgttcgttga tgaccggatt
      181 gccgaccttg acgcccttgg ccgagccggc gtcgatcagg cgggcccgcg agaacggccc
      241 gcgcgtgtcg gagatggagc gggccgtctg catggccacc ggcggctcgg gccggatgcc
      301 cagcatggcc tcgtagcggg cgttgacgtt cttcagggcg atagcctggt cacgccacgg
      361 ggcgagctcg gccagttcgc gcttcaggcg gcggttctcc gagacggcga agaaatagcc
      421 gccgacatag tcggtggcgg atccgaacca gcggaccggg gcggcgaaga caccgccgac
      481 gggaccggcc acggactcca cgccggcgcg gaccggctga tagggcgagg tctgggcccg
      541 ggcgtcgctg atcagcagca ggatgcagcc gatcgcggcc agcacgacgg ccacggccgc
      601 ggtccagacc agcggcacct tgatgttctc gaacggtccg tcgcgaaacg ccacggcgcg
      661 ccttcctggg aaaaagggaa tcagcgggac ccccccgccg acttcagcag cctatcgcaa
      721 aatccggctc tgatgggaac cggtcgcatc ccctgatgac agggatttgc ggcggagttg
      781 gggatcaagc cctagagggc ggaatccagc acgcccttca tccaccgggg atgctcgagc
      841 acgcgaccgc agccgatggc gacgcaggac agcggatcgt cggcgaccga gaccggcagg
      901 ccggtgtggt cgcggatctc ggcgtccaga ccgcgcagca gcgcgccgcc gccggtcagc
      961 atgatgccct tgtcggcgat gtcggcggcc agttccggcg gcgtggcttc cagcgcgacc
     1021 ttcacggcct cgacgatctg ggtcaccggc tcggccaggg cttcggcggc ctgacgttcc
     1081 gagatgcgga cctcgcgcgg gacgccctgc atcaggtcgc ggcccttgac gtcgatcgac
     1141 aggccttcgc cgtcggccgg gatgcgggcg gtgccgatgt ccttcttgat gcgttcggcg
     1201 gtcgtctcgc cgatcagaag gttatggttg cggcgcatgt acgagatgat tgcctcgtcc
     1261 atcttgtcgc cgccgacgcg gaccgagcgc gaatagacga tgcccgacag ggacagcacg
     1321 gccacctcgg tggtgccgcc gccgatgtcg acgaccatgg agccggtcgg ctcgtggatc
     1381 ggcaggccgg cgccgatcgc ggcggccatc ggctcgtcga tcaggccgac gcggcgggcc
     1441 gaggcgttga ggcaggaatc gttgatggcg cggcgttcga cggcggtggc gcccgacggc
     1501 acgcagacga tgaccttggg gttcacgaag cccttgcggt tatgaacctt gcggatgaag
     1561 tgtttgatca tctcctcggc gacttcgaag tcggcgatga ccccgtcgcg catgggacgg
     1621 atggcttcca tatggcccgg ggtgcggccc agcatctgct tggcctctat gcccacggcg
     1681 tggacgatct tccgtccacc gacgtttcga agggcgacca cggacggctc gttcaggacg
     1741 atgcccttgc ccttcatgta gatcagggtg ttggcggtgc cgaggtcgat ggcgatgtcg
     1801 ttggagatcg cgccgaaaat gctggagaac atgggtccgg gataccgttc gttcgggcct
     1861 tggaggggcg tttggtcgtc tcgatccgct tcggcgacgc cccggcgacg cgcatcccca
     1921 cgcaccgcct cctgatcgtt atcgcctgac cgaccttacg gctcgtttgg cagtgtgtga
     1981 cggcgaatac aagcccttcc aagcgtcgct gtcgaaggcg tgaccggagg gtcagtcggc
     2041 ccccgacgcg gcctcgcgcc gtttcaccag ttcgcgcacg cccagcatca gacccagcca
     2101 gaccacggcg acgccggcga ggatcagcga ggtccagacg ccgtcgccgc tgaccgccga
     2161 aaccacggcc ccgccgaaga aggacaccag cgccgtctta ggcaggacgc cgagtgcgca
     2221 gcccgccaga aagcgccaga aggaggcgcg cgccgctccg aacgccatgt tgacgacgat
     2281 gaacggcgcc gatggcacgt tgcggatcat gaagctggcg taaaaggcgt tcttgccgac
     2341 gaagcccttg agtcggccca tggtccgtcc gccgaaccgg tcgaccagcc gcgccgtcgg
     2401 cccgcgtccc agccagtagg tgacgcccgc cgagacgatg gtggcggccc agctgtagaa
     2461 gaagcccatc gacggcccga aggcgaccac gcaggcggcg atgaggatga actggggtgc
     2521 gccgatgaag gcggcggcga tgaacaggac caccgtggcc gcgaaggccc acggacttcc
     2581 gcgatagccc tgcagccagg cctcgagatt ctcctctgcg gccagcccga actggctctt
     2641 gccgatcgcc agcagaccaa gggtcgcgcc cagcagcagc gccgtcgcca gcacggcccg
     2701 ccagcggcgg acttccatgt tgaggatgaa gtcgaggacg cggcgcatga acgatcaatc
     2761 cgccaccggc gcgaacggtc ccaccgtcca cgacttgagg gcccagtcga atccgtcggg
     2821 ccggacccat tcctgacggg aggctgccct gtaggttttg cggtcgcgat agtggatgac
     2881 ggctccctcg atccatgcag acgatgaact cagggctccg accgtccgga cttccacgtg
     2941 gatgcccccc agacggttcg cttgaccgga catgaattcc gagatcgtcc gcggcatagg
     3001 gtctcccggc ccaaagctgt tggtttcctg aatgatctcc ccgttccaat ccagacgggc
     3061 gttctgattg gcatgcccga cccacaggga ccaatcggtc tcgttgattt cctgcacgcc
     3121 gaaggcggtg acgcgcccct tttcgttcat gagggtcgca gccgccacaa ggtcgccctc
     3181 cagtgtcaga caggactcaa gtttggcgac atcgtt
//
LOCUS       DASVVN010000510         5702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_72070, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000510 DASVVN010000000
VERSION     DASVVN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72070"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF686_08275"
     CDS             complement(<1..284)
                     /locus_tag="VF686_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661850.1"
                     /translation="MSQTEPSYVKPRAPALMVLFLTVFINLVGFGVVVPLLPFFATAL
                     NAPAWQITLMFSAYSLGQFFAEPFWGRLSDRIGRKPVLVVTTALNVVGYLL"
     gene            complement(371..892)
                     /gene="infC"
                     /locus_tag="VF686_08280"
     CDS             complement(371..892)
                     /gene="infC"
                     /locus_tag="VF686_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX8661851.1"
                     /translation="MNQPPVKDGPPMNHDIRAPRVLLIDQNGEKQGVMPTSAALEAAE
                     EAGMDLVQIVSTSEPPVCKILDYGKFRFQEQKKKAEARKRQKVVELKEIKLRPNIDVH
                     DYEVKAKAMHRFFDEGDKVKVTLRFRGREMAHPELGMKLLNKVQADFDEIAKVEYAPR
                     MEGRQMIMILAPK"
     gene            complement(1017..1988)
                     /locus_tag="VF686_08285"
     CDS             complement(1017..1988)
                     /locus_tag="VF686_08285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX8661852.1"
                     /translation="MPDPKTAPRVLFVTSNRIGDCVISSGVIREIGRQLPGARITVAS
                     GRPPAPLFRAAPGVERVIILDKKKMAGHWVDLWKQVVGTRWDLVIDIRGSALSYLVPA
                     KRRVVYNRSWETGVRKVEMVSRLMGSSTPLDPEIFLDDQARAEAAAVIDPQLATAPGP
                     IIAMAPIAHQPGKSWPADRWGGLVEMLKADARFDGWRFMLVGGPGDHPPAAPALAAAG
                     ERGIDFVGKGDILASAAAIDRAALFVGNDSGLMHVAAAAGRPTLGLFGPTEWWLYGPW
                     GPRTRTAASNETRGEFAPIEALTTKRVFDAVIDLHTAFIEARSPAKP"
     gene            2058..2813
                     /locus_tag="VF686_08290"
     CDS             2058..2813
                     /locus_tag="VF686_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661853.1"
                     /translation="MSDGIQRLQRPDGETLAFWRIEGEGPTVVWIGGFRSDMEGTKAL
                     ALDAAARERGWNYLRYDHFAHGQSSGDWRRATIGRWREDAIALIDSLEGPVIPVGSSM
                     GGWVALLLALARPERMAGLALVNPAQDFTERLMWPGLADHQRQAILRDGETLIIEEGP
                     GEYVLTLAMFEEARDWLVLDAPIPVAAPVHILQGRADDVVPWEHAVRLVERLMGGDVR
                     LDLIEGGDHRLSGPADLARLTEAIERMRHWALK"
     gene            2840..3058
                     /locus_tag="VF686_08295"
     CDS             2840..3058
                     /locus_tag="VF686_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661854.1"
                     /translation="MRVLVPALLLLAAAGCAPTTGGNRYDNDLQRLTADCTARGGILA
                     PTGQQTGRPETDNVCKITGGASRLTSGG"
     gene            complement(3066..3956)
                     /locus_tag="VF686_08300"
     CDS             complement(3066..3956)
                     /locus_tag="VF686_08300"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX8661855.1"
                     /translation="MTAHPRRLRKAVLPVAGLGTRVLPGTKTTPKELLNVVDRPILSY
                     IVEEAREAGIEHIVFVTGRSKGAIEDYFDHQIELEAQLLAKGKTDILDAMIAELATAG
                     EMSFTRQMQPLGLGHAVWCARDIIGDEPFAVLLPDVIVDSQPGALKQLADVYAEVGGN
                     VIGVEAVPETETHKYGIVDPVTGPGGRDGRHIRMKGMIEKPPRGEAPSNLSISGRYIL
                     QPEIFGILENQPRGAGGEIQLTDGMARLMKDQTFTAVEYEGVTHDCGDKIGLLRANVA
                     LALKRPDLGEAARAAITALL"
     gene            complement(4167..>5702)
                     /gene="glmS"
                     /locus_tag="VF686_08305"
     CDS             complement(4167..>5702)
                     /gene="glmS"
                     /locus_tag="VF686_08305"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX8661856.1"
                     /translation="VLVHNGIIENFAELKAELAEDGRVFESQTDTEIVAHLLDRELGR
                     ANDPLVAFKTVLDRLTGAYALAVLIEGEDGLIMGARRGSPLVVGWGEGEMYLGSDALA
                     VGPFTQKISYLEEGDYVGVDRSGARMFDVHGAPVERPIVQVSASSALVEKGEYRHFME
                     KEIHEQPDSVQHTLSHYLDFVNGRAKTDSADFAAIDRIQIVACGTAFYAGQIGRYAFE
                     KLANLPCDVEIASEFRYRNPALTPGTLAVAVSQSGETADTLASLSWCKAKGLKTAALV
                     NVHSSSMAREAGHLWPTHAGPEIGVASTKAFTAQVAALLALAVAAGVQRGHIDAVREA
                     ELVKALFEAPRLIAEAMGMQDDLKAVAHDIAKATDVLFLGRGAMFPLAMEGALKLKEI
                     SYIHAEGYAAGELKHGPIALVDELTPIVALAPLDEVFEKTASNLQEVAARGGPVIMIA
                     PKSAPDPHGAGIRRVDAPECHSLIAPLIYAIPVQLLAYYTAVQKGTDVDQPRNLAKSV
                     TVE"
BASE COUNT          901 a         1867 c         1960 g          974 t
ORIGIN      
        1 agcaggtagc cgaccacgtt cagcgcggtg gtgacgacca gcaccggctt gcgcccgatg
       61 cggtcggaca ggcggcccca gaacggctcg gcgaagaact ggcccaggga ataggccgag
      121 aacatcaggg tgatctgcca ggccggcgca ttcagcgcgg tggcgaagaa gggcagcagc
      181 ggcaccacca cgccgaagcc gaccagattg atgaagacgg tcaggaacag caccatcagg
      241 gccggggccc ggggcttcac gtaggacggc tcggtctggc tcatggtcaa gccttagcct
      301 gtcacgaggg atacgaaaac gccccggccg tttccggtcg gggcgttttg caatcctgag
      361 gtcttcgggc ctatttcggc gcgaggatca tgatcatctg gcggccttcc atacgcggcg
      421 cgtattcgac cttggcgatc tcgtcgaagt cggcctggac cttgttcaac agcttcatgc
      481 ccagttcggg gtgggccatc tcgcggccgc ggaagcgcag cgtcaccttg accttgtcac
      541 cctcgtcgaa gaagcggtgc atggccttgg ccttcacctc ataatcgtgg acgtcgatgt
      601 tggggcgcag cttgatctct ttgagctcga cgaccttctg acgcttgcgc gcctcggcct
      661 tcttcttctg ctcctggaag cggaatttgc cgtagtcgag aatcttgcag accggcggct
      721 ctgacgtcga gacgatctga accaggtcca tgccggcctc ttcggcggct tccagcgcag
      781 cggacgtcgg catgacgcct tgtttctcgc cgttctgatc aatcagcaga acgcgcggcg
      841 cgcggatatc gtggttcatg gggggcccgt ccttgacggg cggctggttc atcggacggc
      901 gaatgagcgt cggttccttg cgtggaagaa gaagtagagt tctgacaggg cggagtacgc
      961 gacggttcgc cgcgttcccc tccagcggcc ctatatgacc gcgaacgccc ttctcttcaa
     1021 ggtttggcgg ggcttcgggc ttcgatgaag gcggtatgca ggtcgatcac ggcgtcgaag
     1081 acacgcttcg tcgtcagcgc ctcgatggga gcgaactcgc cgcgggtctc gttggaggcc
     1141 gccgtgcggg tccttgggcc ccaaggtccg tagagccacc actcggtcgg accgaacagg
     1201 cccagcgtcg ggcggccggc ggcggcggcc acgtgcatca ggccggaatc gttgccgacg
     1261 aacagcgccg cccggtcgat ggcggcggcc gaggccagga tgtcgccctt gccgacgaag
     1321 tcgatcccgc gttctcccgc cgccgccagg gcgggggccg cgggtggatg atcgccgggg
     1381 ccgcccacca gcatgaagcg ccagccgtcg aaccgcgcgt cggccttgag catctcgacc
     1441 agaccgcccc agcggtccgc gggccagctc ttgcccggct ggtgcgcgat cggcgccatg
     1501 gcgatgatcg gcccgggcgc tgtcgccagc tgagggtcga tcacggcggc ggcctcggcc
     1561 cgcgcctgat cgtcgaggaa gatttccggg tccagcggcg tcgacgaacc catcagacgc
     1621 gacaccatct cgaccttgcg caccccggtc tcccacgacc ggttgtagac cacgcgccgc
     1681 ttcgcgggga ccagatagct gagggccgag ccgcggatgt cgatgaccag atcccagcgc
     1741 gtcccgacca cctgtttcca cagatcgacc cagtgtccgg ccatcttctt cttgtcgagg
     1801 atgatgaccc gctcgacgcc gggcgccgcg cggaacaggg gcgcgggcgg acggccgctg
     1861 gcgacggtga tccgggcgcc gggcagctgg cggccgatct cgcggatcac gcccgacgaa
     1921 atcacgcagt cgccgatgcg gttggaggtg acgaacagaa ccctgggggc ggtcttggga
     1981 tcgggcacgc gctttggcct tcgctagata tccggggttc gctatcagtc cgcgacggtt
     2041 ttcgcgagag gcgggtcatg agcgacggca ttcagcggct gcaacgcccg gacggcgaga
     2101 ctctcgcctt ctggcggatc gagggcgaag gtccgacggt cgtctggatc ggcggcttcc
     2161 gctccgacat ggaggggacc aaggcgctgg cgctggacgc ggcggcgcgc gagcggggct
     2221 ggaactacct ccgctacgac catttcgccc atggccagtc gagcggcgac tggcgcaggg
     2281 ccaccatcgg ccgctggcgc gaggacgcca tcgccctgat cgacagcctc gaaggtccgg
     2341 tcattccggt cggctcgtcg atgggcggct gggtcgcctt gctgctggcg ctggcgcggc
     2401 ccgagcggat ggcggggctg gcgctggtca atccggccca ggacttcacc gaacggctga
     2461 tgtggccggg cctcgccgac caccaacgtc aggccatcct gcgcgacggc gagacgctga
     2521 tcatcgagga aggaccgggc gaatacgtcc tgaccctggc catgttcgag gaggcgcggg
     2581 actggctggt gctggatgcg ccgatcccgg tcgccgcgcc cgtgcatatc cttcagggcc
     2641 gggccgatga cgtggtgccg tgggagcacg cggttcgtct ggtcgaacgt ctgatgggcg
     2701 gcgacgtccg gctggacctg atcgagggcg gcgaccaccg tctgtccggc cctgccgatc
     2761 tggcgcgact gacagaggcg attgaacgga tgcggcattg ggcgcttaaa taaggaacgc
     2821 atcgcaagga gactccccca tgcgcgtcct cgtccccgcc ctgcttctgc ttgccgccgc
     2881 cggctgcgct ccgaccacgg gcggaaaccg ctatgacaat gacctgcagc gcctgaccgc
     2941 cgactgcacc gcacgcggcg gcatcctggc cccgacgggc cagcagaccg gccggccaga
     3001 gaccgacaac gtttgcaaga ttaccggcgg cgcatcgcgg ctgaccagcg gcggctaggt
     3061 cacggttaca gaagcgcggt gatcgccgcg cgggccgcct cgccaaggtc cggacgtttc
     3121 agcgccaggg ccacattggc gcgcagcagg ccgatcttgt cgccgcagtc gtgcgtgacg
     3181 ccctcgtatt cgacggcggt gaaggtttgg tccttcatca gccgcgccat gccgtcggtc
     3241 agctggatct cgccgcccgc gccgcgcggc tggttttcca gaatcccgaa gatctccggc
     3301 tgcaggatgt agcggcccga gatcgacagg tttgacggcg cctcgccgcg cgggggcttt
     3361 tcgatcatcc ccttcatgcg gatgtgacgg ccatcgcgcc cccccggtcc agtgacgggg
     3421 tcgacgatgc cgtatttgtg ggtttcggtc tcgggcacgg cctccacgcc gatgacgttg
     3481 ccgccgacct cggcatagac gtcggccagc tgcttcagcg ctccgggttg ggagtccacg
     3541 atcacatcgg gcagcaggac ggcgaagggc tcgtcgccga tgatgtcccg ggcgcaccag
     3601 accgcatggc cgaggcccag cggctgcatc tgccgcgtga agctcatttc accggccgtc
     3661 gccagttcgg cgatcatggc gtcgaggatg tctgtcttgc ccttggccag gagctgggct
     3721 tccagctcga tctggtgatc gaaatagtcc tcgatcgccc ccttggaccg gccggtgacg
     3781 aagacgatgt gctcgatccc ggcctcgcgc gcctcctcga cgatgtagct caggatcggc
     3841 cggtcgacga cgttcagcag ttccttgggc gtggtcttgg tgccgggcag gacacgcgtg
     3901 ccgaggccgg cgacgggcag aacggccttg cggaggcggc gggggtgagc ggtcatgcgg
     3961 aatcctgaat gtgcatggga tgcaacctaa cgcccaatac gaaacttcgt acccctcctt
     4021 tcgtcatccc ggcgaaggcc gggacctagc ggcgccgaag gcgatctgtt caggcaggct
     4081 gtctcaggca gatgtgtccg cgacagtttc gcgctctcgc gcgccgctgg gtcccggcct
     4141 tcgccgggat gacgacaaat acagcgctat tcgacagtga cggacttggc cagattgcgg
     4201 ggctgatcca cgtccgttcc cttctgcacc gccgtgtaat aggccagcag ctgcacgggg
     4261 atggcgtaga tcagcggtgc aatgagcgag tgacactcgg gcgcgtcgac gcggcgtatg
     4321 ccggcgccat ggggatcggg cgcggatttc ggtgcgatca tgatcaccgg cccgccccgc
     4381 gcggcgactt cctgcaggtt ggaagcggtc ttctcgaaca cctcgtccag cggggccaag
     4441 gccacaatcg gggtcagctc gtccaccagg gcgatcggtc cgtgcttcaa ctcgcctgcc
     4501 gcgtagccct cggcgtggat gtagctgatc tccttcagct tcagggcgcc ttccatggcc
     4561 agcgggaaca tggcgccgcg gccgaggaac aggacgtcgg tcgccttggc gatgtcgtgg
     4621 gcgacggcct tcagatcgtc ctgcataccc atggcctcgg cgatcagacg cggggcttcg
     4681 aacaaggcct tgaccagttc ggcctcgcgc acggcgtcga tatggccgcg ctgcacgccc
     4741 gccgcgacgg cgagggccag cagggccgcg acctgggcgg taaaggcctt ggtcgaggcg
     4801 acgccgatct cgggaccggc atgggtgggc cacagatgcc cggcctcacg cgccatcgag
     4861 gacgagtgga cgttgaccag ggcggcggtc ttgagaccct tggccttgca ccacgacagg
     4921 ctggccaagg tgtcggctgt ctcgcccgac tggctgacgg cgacggccag cgtccccggc
     4981 gtcagggccg gattgcggta gcggaattcc gaagcgatct cgacgtcgca gggcaggttg
     5041 gccagcttct cgaacgcata acggccgatc tggccggcgt agaaggcggt gccgcaggcc
     5101 acgatctgga tgcggtcgat agcggcgaaa tcggcggaat cggtcttggc cctgccgttc
     5161 acgaagtcga ggtagtggga cagagtgtgc tggacgctgt ccggctgctc atggatttcc
     5221 ttctccatga agtgccggta ctcgcccttt tcgaccaggg ctgacgacgc cgagacctgc
     5281 acgatcggcc gttcgaccgg ggcgccatgg acgtcgaaca tgcgcgcgcc gctgcggtcg
     5341 acgccgacgt agtcgccctc ctccagatag ctgatcttct gggtgaaggg gcccaccgcc
     5401 aacgcgtccg agccgagata catctcgccc tcgccccacc cgacgaccag cggactgccg
     5461 cgccgtgcgc ccatgatcag gccgtcctcg ccctcgatca gaaccgcaag cgcataggcg
     5521 ccggtgagcc ggtccagcac cgtcttgaag gcgaccagcg gatcattggc gcggcccagt
     5581 tcgcggtcga gcaggtgggc gacgatctct gtgtcggtct gactctcgaa cacgcggccg
     5641 tcctcggcca gctcggcctt cagctcggcg aaattctcga tgatgccgtt gtggaccagc
     5701 ac
//
LOCUS       DASVVN010000511         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_72087, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000511 DASVVN010000000
VERSION     DASVVN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72087"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..556
                     /locus_tag="VF686_08310"
     CDS             107..556
                     /locus_tag="VF686_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661857.1"
                     /translation="MRVLPALLAAAALLSGCATPIEPGPDAPKMADNGNDCAVIVAIA
                     KEHYRFNATDNRPPPIRFEGDFAPRCDWSRHGLAFTAYDADAPGDPRERMRWVSFARP
                     TYDGRGAVVETGIMHGPLAGMGYECRVISGFAGWTVPEGACRNTWVS"
     gene            complement(560..1195)
                     /locus_tag="VF686_08315"
     CDS             complement(560..1195)
                     /locus_tag="VF686_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661858.1"
                     /translation="MLAALPFLLAVLVVQDPDPYPRIRLENHLPALDNRSDTHEPGYR
                     AVRANAEDMLGLSMPMVEMSVVDPNRIMSASVVFSRVQIVEDGGRRGVWFARLRAARG
                     RASTEVFADARTCPGVVASLEALNRLPDLNPKVPWLDLVTGTSNSAVQMDDIILDDID
                     YSVSLRASFDGSEHTDQITVSGGSYSPFAPIIVESLDRLKACWTETPPPAR"
     gene            1303..>2215
                     /locus_tag="VF686_08320"
     CDS             1303..>2215
                     /locus_tag="VF686_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX8661859.1"
                     /translation="MTDIAPETIDRPLTQDGPALRLWMIDASAYIFRAYHALPPLTRK
                     SDGLPVGAIQGYCNMLWKLLQDMKGADGPTHLVAIFDHSEKTFRNDLYDQYKANRSAP
                     PEDLIPQFPLVREATAAFGVACLELPGYEADDLIATYACKARDAGGEAVIVSSDKDLM
                     QLIGGGVVMWDPMKDRRLEEDAVMEKFGVTPDKMVDLQALIGDSVDNVPGAPGIGPKT
                     AAQLLDEYGDLDTLLERAGEIKQPKRRETQINFRDQILLSRELVRLTCDAPAVEPIDD
                     FAVRDPDPEVLGAFLDEMEFRSLRTRVG"
BASE COUNT          376 a          791 c          711 g          337 t
ORIGIN      
        1 cgcccggaat gcgggccgcc ccgcccttgt tcacctgatc acctcggccg aggatatcgc
       61 ccccaaccgc accatcaccg gccttcgctc gcaataagga gccgccatgc gcgttcttcc
      121 cgccctgctc gccgccgcgg cgctcctcag cggctgcgcc acgcccatag aacccggccc
      181 cgacgccccg aagatggctg acaatggcaa tgactgcgcc gtcatcgtcg ccattgcgaa
      241 ggagcactac cggtttaacg ccaccgacaa ccgcccgccg ccgatccggt tcgagggcga
      301 cttcgccccg cgttgcgact ggagccgtca tggcctcgcc ttcaccgcgt atgacgccga
      361 cgcccccggc gatccacgcg aacggatgcg ctgggtcagt ttcgcccggc cgacctatga
      421 cggacgcggc gccgtggtgg agacaggcat catgcacggt cccctggctg gcatgggcta
      481 tgagtgccgg gtcatttccg gcttcgctgg ctggacggtc ccggaaggtg cctgccgaaa
      541 cacctgggtc tcctgatcgt cagcgtgcgg gcggcggcgt ttctgtccag cacgctttca
      601 gccggtcgag gctttcgacg atgatcggcg cgaacggcga gtaggacccg cccgagacgg
      661 tgatctgatc cgtgtgttca gaaccgtcga agctggcgcg aagactgacg ctgtaatcga
      721 tgtcgtccag tatgatgtcg tccatctgga cggcggaatt cgacgttccg gtcacgaggt
      781 ccagccaggg cactttcggg ttcaggtccg gcaggcggtt cagcgcctca agggaggcga
      841 cgacgccggg acaggtgcgg gcgtccgcga agacctcggt agaggcgcgg ccccgggcgg
      901 ctctcaatcg ggcgaaccag acgccgcggc gtccgccgtc ctcgacgatc tgaacgcggc
      961 tgaagacgac agacgccgac atgatccggt tcgggtcgac cacagacatc tcgaccatgg
     1021 gcatactgag ccccagcata tcctccgcat tcgcgcgcac ggcgcgatag cccggctcgt
     1081 gggtgtccga ccggttgtcc agcgccggca ggtggttctc cagccggatg cgcggataag
     1141 ggtccgggtc ctgaacgacc agcactgcga gcagaaaggg aagggcggca agcatggcga
     1201 tctcctggag ccggcagcag atggcgcgcc ggggtcgcgg tcaatgacga atccggcagg
     1261 ggtcgaggtg acgcggtccg tgacgccgcc tatgttcgcc gcatgaccga catcgcgccc
     1321 gagaccatcg accgccccct cacccaggac ggccccgcgc tgcggctgtg gatgatcgac
     1381 gcctcggcct acatcttccg cgcctaccac gccctgccgc ccctgactcg caagtccgac
     1441 ggcctgccgg tgggcgcgat ccagggctac tgcaacatgt tgtggaagct gctgcaggac
     1501 atgaagggcg ccgacggccc cacccatctg gtcgccatct tcgaccactc cgagaagacc
     1561 ttccgcaacg acctgtacga ccagtacaag gccaaccgct cggccccgcc cgaggacctg
     1621 atcccccagt tcccgctggt ccgcgaggcc accgcggcct tcggcgtcgc ctgtctggaa
     1681 ctgcccggct acgaggccga cgacctgatc gccacctatg cctgcaaggc gcgcgacgcg
     1741 ggcggcgagg ccgtcatcgt cagctccgac aaggacctga tgcagctgat cggcggcggc
     1801 gtggtcatgt gggatccgat gaaggaccgg cggctcgagg aagacgccgt catggagaag
     1861 ttcggcgtca cgcccgacaa gatggtcgac ctccaggccc tgatcggcga cagcgtcgac
     1921 aacgtccccg gcgcccccgg catcggcccc aagaccgccg cccagctgct ggacgaatac
     1981 ggcgacctcg acacgcttct ggagcgcgcg ggcgagatca aacagccgaa acgccgcgag
     2041 acccagatca atttccgcga tcagatcctg ctcagccgcg aactggtgcg cctgacctgc
     2101 gacgcccccg cggtcgagcc gatcgacgac ttcgccgtcc gcgaccccga ccccgaggtg
     2161 ctcggcgcct tcctcgacga gatggagttc cgctcgctgc gcacccgcgt cggcg
//
LOCUS       DASVVN010000512         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_19498, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000512 DASVVN010000000
VERSION     DASVVN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19498"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VF686_08325"
     CDS             complement(<1..699)
                     /locus_tag="VF686_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8661860.1"
                     /translation="MRPALIIAAFAALASSPVMSQSVSPSVPNAAPWDQPFLPPAPEW
                     DGASRALLRGASDPWVTAFETDPDHDFSPDYADTRAWFDRLDAASDLIRIEQFGVSPE
                     GRPIYAVIASKDGAAFDPAKPVLLAQAGIHPGEIDGKDAGMMLLRDIAFYGKSDLLDR
                     VNLILIPILSVDGHERASAYSRPNQRGPRIQGWRNTATNQNLNRDYLKLDQPEMRAVR
                     GLILKYRPDLYVDIH"
     gene            complement(753..1373)
                     /locus_tag="VF686_08330"
     CDS             complement(753..1373)
                     /locus_tag="VF686_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661861.1"
                     /translation="MSAFEFFFSFYGLLLGLSVAELVGGFARVLHERHRIRFGWLTPL
                     LAVFVAMDVATFWSQAWVIFRGAPFNLSLLFLGLVVAATFYVAASVTFPRVTAEGVSD
                     RIDLDEHFWAHRRLVFTCVMAANAIVALVFTGLAAANAGFAELATAPRLWIGMAIFVV
                     GTATAAFAPWRRVAVAALLVVLLYSAWNVARSAASLIAAGGWSPPG"
BASE COUNT          268 a          474 c          573 g          201 t
ORIGIN      
        1 gtggatgtcg acgtagaggt cgggccggta tttcaggatc aggccgcgca ccgcccgcat
       61 ctcgggctgg tccagcttga ggtagtcgcg gttcaggttc tggttggtgg cggtgttgcg
      121 ccagccctgg atgcgcggcc cgcgctggtt ggggcggctg taggcgctgg cccgctcgtg
      181 gccgtcgacc gacaggatcg ggatcaggat caggttgacc cggtccagca gatccgactt
      241 gccgtaaaag gcgatgtcgc ggagcagcat catgcccgcg tccttgccgt cgatctcgcc
      301 cgggtggatg ccggcctggg ccagcagcac gggcttggcc ggatcgaagg cggcgccgtc
      361 cttcgaggcg atcacagcgt agatcggccg gccctcgggc gagacgccga actgttcgat
      421 gcggatcagg tccgaggcgg cgtcaagccg gtcgaaccag gcgcgtgtgt cggcatagtc
      481 ggggctgaag tcgtggtccg gatccgtctc gaaggccgtg acccaggggt cggacgcgcc
      541 gcgcagcagg gcgcggctgg cgccgtccca ctcaggcgca ggcggcagga agggctgatc
      601 ccatggcgcg gcgttgggaa cggacggcga aacggactgg gacatgacgg gactcgatgc
      661 gagcgcggcg aacgcggcga tgatcagggc tgggcgcatg gcgtcttcct cggccaaatg
      721 tgaacgtccg acaagccttc cgcagagcgg cgtcagccgg gcggggacca gccgcccgca
      781 gcgatcaggc tcgccgccga ccgcgccacg ttccaggcgc tgtagagcag aaccaccagc
      841 agggcggcca cagccacccg ccgccagggc gcgaaggcgg ccgtggccgt gcccacaacg
      901 aagatggcca tgccgatcca gagccggggg gctgtcgcca gttcggcgaa gccggcgttc
      961 gcggcggcca gacccgtgaa aaccagggcg acgatggcgt tggcggccat gacgcaggtg
     1021 aagaccagcc gccggtgcgc ccagaaatgc tcgtcgagat cgatccggtc gctgacgccc
     1081 tcggcggtca cgcgcgggaa ggtgacgctg gcggcgacgt agaaggtggc cgcgaccacc
     1141 agtccgagga acagaagcga aaggttgaac ggcgcgccgc ggaagatgac ccaggcctgg
     1201 gaccagaagg tcgcgacatc catggccacg aagacggcca gcaggggcgt cagccagccg
     1261 aaccggatcc ggtggcgctc atgcaggacg cgggcgaagc cgccgaccag ctcggcgacc
     1321 gacaggccca gcagcagtcc gtagaagctg aagaagaact cgaaggcgct cattgggcgc
     1381 ttacgccggg gtggttgggc gcgacgaggg caagactgtc cggcatgatc gccgaactga
     1441 cggcgctggc cgcgaatatc aggatgttca ggccgatgag tatggcgaac agccgcggcc
     1501 gtttcgccgc cgccgc
//
LOCUS       DASVVN010000513         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_97371, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000513 DASVVN010000000
VERSION     DASVVN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97371"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF686_08335"
     CDS             <1..410
                     /locus_tag="VF686_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX8661862.1"
                     /translation="RGDRLAFEPRLPSLPAVLINPGVPSPTGAVYRAYDEGSAGLADR
                     PEPPGSWATSAVIDWLSEQRNDLEAPAVALQPVIGEALTAAAQLPGVRLARMSGSGAT
                     VFALFDTADQAAAAARRVTETHHDWWVRPAALR"
     gene            530..1549
                     /locus_tag="VF686_08340"
     CDS             530..1549
                     /locus_tag="VF686_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8661863.1"
                     /translation="MLRLHLLTATAAAALLAAPAALAQTAPTPPSPSPVPATPATPAT
                     APAAAPANSAPAATPAEPADEATPPPQAPAAQVPAAPPATQPARPATPAAAGNVIDVL
                     TAQGNFTTLLAALNQAQLTETLRSRPAVTIFAPTDEAFAALPEAERSRLMDPANAQEL
                     RNLLLYHVIVADVSSSQITGTKGGVQTANRTRVLLDGTGEALRIDGALITRADIDASN
                     GAIFAIDKVLNPAQSMAPAEAPAPAAAPAATAPATTPAPATATVPPGATATTRPATPG
                     TNAPNGQPAATTTTIAAPPVPNPTDGQVDRQPDPSRATPPVDTPDPADDDDDTPTPPP
                     PTPGR"
BASE COUNT          223 a          678 c          503 g          208 t
ORIGIN      
        1 agcgcggcga ccggctggcc ttcgagccgc gcctgccgtc cttgcccgcc gtgttgatca
       61 atccgggcgt cccgtcgccc acgggcgcgg tctatcgggc ttacgacgag ggatcggcgg
      121 gactggccga ccgtcccgaa ccgcccggta gttgggcgac gtcggcggtt atcgactggt
      181 tgtccgagca gcggaacgac ctggaagccc cggcggtcgc gctccagccc gtcattggtg
      241 aagccctgac ggcggcggcg cagcttccgg gcgtccgcct tgcccggatg tcgggttcgg
      301 gcgccaccgt cttcgccctg ttcgacacgg cggatcaggc cgctgccgcc gcccggcgcg
      361 tgacggagac ccatcacgac tggtgggtga ggcctgcggc tctgcgctag cgccgttcac
      421 ccaattggaa ctgaggcccc gcctgcgtct ttcacagtgc aggtttcggg cgcctctggc
      481 cgcgtcctgc tccctttgcg gccacccgcc caaggaaagg gatattgtca tgcttcgcct
      541 ccacctgctg accgctaccg ccgcggccgc gcttctcgcg gcgcccgcgg ccctggccca
      601 gaccgccccc acgccgccgt ccccgtcgcc ggtccccgcc actccggcca cgccggcaac
      661 agcgcccgct gcagccccgg ccaatagcgc gcccgccgcc acgcccgccg aacccgctga
      721 cgaggcgacg cctccgccgc aggcgcccgc cgcccaggtt cccgccgcgc ctcccgccac
      781 ccagcccgcc cggcccgcga cgcccgccgc cgccggaaac gtcatcgacg tcctgaccgc
      841 ccagggcaat ttcaccaccc tgctggcggc gctgaaccag gcccagctga cggagaccct
      901 caggtcgcgc ccggctgtca ccatcttcgc cccgaccgac gaagccttcg cggccctgcc
      961 cgaggccgaa cgaagccggt tgatggaccc ggccaacgcc caggaactgc gcaatctgct
     1021 gctttatcac gtgatcgtcg ccgacgtttc atcgtcccag atcacgggga ccaagggcgg
     1081 cgtccagacc gcaaaccgca cgcgcgtcct gctggacggc accggcgagg cccttcgcat
     1141 cgacggagcc ctgatcacgc gggcggacat cgacgcttcc aatggcgcga tcttcgccat
     1201 cgacaaggtt ctgaaccccg cccagtccat ggcacccgcg gaagctccgg cgccggcggc
     1261 cgcccctgcg gcgacggctc cggcaaccac gccggctcca gcgaccgcca ccgtcccgcc
     1321 cggagccacg gcgaccaccc gccctgccac gccgggaacc aatgcgccca acggccagcc
     1381 cgccgcgacc acgacgacca tcgccgctcc gcccgtgccc aatccgaccg acgggcaggt
     1441 ggacaggcag ccggacccgt cgcgcgcgac cccgccggtg gatacgcccg atcccgccga
     1501 cgatgacgac gacacgccca cgccgcctcc gccgacgccc ggccgctgat ccggcgactg
     1561 cctgcaagga cggcggcgga cccgcgtccg ccgccgtttc gctgtctgct gc
//
LOCUS       DASVVN010000514         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_390611,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000514 DASVVN010000000
VERSION     DASVVN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390611"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..349)
                     /locus_tag="VF686_08345"
                     /pseudo
     CDS             complement(32..349)
                     /locus_tag="VF686_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            complement(562..873)
                     /gene="rpsJ"
                     /locus_tag="VF686_08350"
     CDS             complement(562..873)
                     /gene="rpsJ"
                     /locus_tag="VF686_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX8661864.1"
                     /translation="METQNIRIRLKAFDHRVLDQATGDIADTARRTGALIRGPIPLPT
                     RIEKFTVNRSPHVDKKSREQFEVRTYKRLLDIVQPTPQTVDALMKLDLAAGVDVEIKL
                     A"
     gene            complement(1067..>1567)
                     /locus_tag="VF686_08355"
     CDS             complement(1067..>1567)
                     /locus_tag="VF686_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX8661865.1"
                     /translation="TGRVETGIVKVGEEVEIVGIKDTKKTVVTGVEMFRKLLDQGQAG
                     DNIGALLRGVARTDVERGQVLAKPGSITPHTDFQAEVYVLSKDEGGRHTPFFANYRPQ
                     FYFRTTDVTGEVVLPEGTEMVMPGDNVNIGVRLIAPIAMDQGLRFAIREGGRTVGAGV
                     VATITK"
BASE COUNT          249 a          541 c          506 g          271 t
ORIGIN      
        1 tgccttgggt gcggccctgg atgtcgacct actgcccggc gacataatgg tcggccgaca
       61 gctcggcgcc cacgtcgagc agcgcatcgc cgtcgacgcg gaactcggcg aggatcgcct
      121 tgggctccac ctcggccttg ccgaaatggc cgcgctgcgg cttggacagg ttcttcgcct
      181 tggcggtgcc ggcgccgagc tggacggccg tatagccgtc gccgtcgctg accttacgcg
      241 ccaccacctg gacgtcttcc aacgccagga cggtgaccgg cacgtgacgg ccgtcctcct
      301 ggaacaggcg ggtcatcccc attttcttcg cgatcacgcc cgtgcgcatg accaaactcc
      361 tcatacagag gccccgcggc acgattaccg ggggcatgtc agcccgaaca tgatgcggcc
      421 ccgcctgggc ttgtgctcca cccgaaggcg gaacggcggg gacgcggccg ggagcatgct
      481 cccgcggtat caccctattt ccgagtccct gcgacagcag cgacccagct gggctcccgc
      541 tttcgcagga gcacagggcc ctcaggccaa tttaatctcc acgtcgacgc cggctgcgag
      601 gtcgagcttc atcagcgcgt cgaccgtctg cggcgtcggc tgcacgatgt cgagcagccg
      661 cttgtacgtc ctcacctcga actgctcgcg cgacttcttg tcgacgtgcg gcgaacggtt
      721 gacagtgaac ttctcgatcc gcgtcggcag cgggatgggt ccgcggatga gagcacccgt
      781 gcgacgcgcg gtgtcggcga tgtcgcccgt ggcctggtcc aggacacgat gatcgaacgc
      841 cttcaggcga atccggatat tctgcgtttc catacctgct accgatgcca aagagccggc
      901 cccgaggggc aaaccaacca aacgcgtcgc cccggcgcgg gccggggtcc cagcaggcga
      961 aaggcgccga gccttccgcc ccgtgagatc ccagccttgg gccgggatga cgaactaaaa
     1021 gcgaaccgcc cgaatctccc gacccgggcg gctgcgcgtg tctctactac ttcgtgatcg
     1081 tcgcgacaac ccccgcgccg acggtccggc cgccttcgcg gatggcgaag cgcaggccct
     1141 ggtccatcgc gatcggggcg atgaggcgga cgccgatgtt cacattgtcg ccgggcatga
     1201 ccatctcggt cccctccggc agcaccactt cgccggtcac gtcggtcgtg cggaagtaga
     1261 attgcggacg gtaattggcg aagaacggcg tgtgacggcc gccttcgtcc ttcgagagga
     1321 catagacctc ggcctggaag tcggtgtgcg gggtgatcga gccgggcttg gccagaacct
     1381 ggccgcgctc gacgtcggtg cgggcgacgc cgcgcagcag ggcgccgata ttgtcgccgg
     1441 cctggccctg atcgagcagc ttgcggaaca tctcgacgcc ggtgaccacc gtcttcttgg
     1501 tgtccttgat cccgacgatc tcgacttcct cgccgacctt gacgattccg gtctcgactc
     1561 ggccggt
//
LOCUS       DASVVN010000515         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_284555,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000515 DASVVN010000000
VERSION     DASVVN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284555"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VF686_08360"
     CDS             <1..1039
                     /locus_tag="VF686_08360"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8661866.1"
                     /translation="LARPDGPDDPVILSLRSALEQPDLHPDDRAELGFALGKALDQVE
                     AWDAAFAAYAEANAASRAGDPERAVYDHAAHERFVDRLIAAFPEPAAPAASGGRPPVF
                     ICGMFRSGSTLAEQILAGHSRVTPGGELGLVPALARTLDPYPEAASAEAIDRLRTLYL
                     NGLNASRPGADVVTDKRPDNFLHIGLIKAMFPDARIVHTVRDPLDTIVSNWFLHLDRS
                     MAHALDLDDLVHWHGQYRRLMAHWKRLYPDILDLDYDALVVEPRPAIEGLLSYLGLEW
                     EEGVLDFHKAETGVRTASVWQVREPLYRRASGRWRNYEKHLAGVRAGLDLPSPLAGEG
                     GSRSETDEGSR"
     gene            1149..1925
                     /locus_tag="VF686_08365"
     CDS             1149..1925
                     /locus_tag="VF686_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8661867.1"
                     /translation="MSEPTPPVVILDKPQMAENIGAVARVMANFGLSELRLVAPRDGW
                     PQERAWATASGADWVLEGVRVFDSVADAVADLNTVYAATARPRETRMAVRTPRETARI
                     LFDDRTSGLNVGLLFGGERAGLETSDIALTQGIVTIPIDPRHHSLNLAQAVAINAYEW
                     RTTVLDAPPPRFREGDPPASGALMLGMYEHLERELDDGGFFHPPEKRPSMVQNLRVML
                     GRSAFSEQEVATFRGVITALSKGRGRVLAKLAAKKAAGEG"
     gene            complement(1922..>2055)
                     /locus_tag="VF686_08370"
     CDS             complement(1922..>2055)
                     /locus_tag="VF686_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX8661868.1"
                     /translation="AGLAGARTVEVNLEPTPGRRLFDEGVYGPATQMVPAFVHNLLA"
BASE COUNT          304 a          679 c          763 g          309 t
ORIGIN      
        1 gctggcgcgg ccggacgggc cggatgatcc ggtcatcctg agcctgcgct cggccctcga
       61 gcaaccggac ctgcacccgg acgaccgggc cgagctgggg ttcgcccttg ggaaggctct
      121 ggatcaggtc gaggcctggg acgccgcctt cgccgcctat gccgaggcca acgccgccag
      181 ccgggccggg gacccggaaa gggctgtcta tgaccacgcg gcgcacgagc ggttcgtcga
      241 ccggctgatc gccgccttcc cggagcccgc cgcgcccgcg gcgtcgggcg gcaggccgcc
      301 ggtgttcatc tgcggcatgt tccgctcggg ctcgacgctg gccgaacaga ttctggcggg
      361 tcacagccgg gtcacgccgg gcggagagct ggggctggtc ccggcgctgg cgcggacgct
      421 ggacccctat cccgaagccg cgagcgcgga ggccatcgac cggctgcgca ccctgtatct
      481 gaatggcttg aacgcgtccc ggcccggcgc cgatgtggtc accgacaagc ggcccgacaa
      541 cttcctgcac atcggcctga tcaaggcgat gttcccggac gcgcgcatcg tccacacggt
      601 gcgagatccg ctggatacga tcgtgtccaa ctggttcctg catctggacc ggtcgatggc
      661 gcatgcgctg gatctggacg atctggtcca ctggcacggc cagtaccggc ggctgatggc
      721 gcactggaag cggctgtatc ccgacattct cgacctcgac tacgacgccc tggtggtcga
      781 gccgcggccg gcgatcgagg ggctgctgag ctatctgggg ctggagtggg aggagggggt
      841 gctggacttc cacaaggctg aaacgggggt gcggaccgcc agcgtctggc aggtgcgcga
      901 gccgctgtac cgccgggcgt cggggcgctg gcggaactat gagaagcatc tggcgggggt
      961 gagggccggg ctcgatcttc cttctcccct tgcgggagaa ggtggctcgc gaagcgagac
     1021 ggatgagggg tctcgctgac ctgacacttc gaataccccg ccgatcgaac cggatggagc
     1081 ggagagaccc ctcatccggc ccttcgggcc accttctccc gcaaggggag aagggatatg
     1141 tgcgtcgcat gtccgaaccc acgccccccg tcgttatcct cgacaagccc cagatggccg
     1201 agaacatcgg cgccgtggcg cgggtgatgg ccaatttcgg cctgtcggaa ttgcgcctcg
     1261 tggcgccgcg cgacggctgg cctcaggagc gggcctgggc cacggcttcg ggcgccgact
     1321 gggtgctgga gggggtcagg gtgttcgaca gcgtcgccga tgctgtggcc gatctgaaca
     1381 ccgtctatgc ggccacggcc cggccccgcg agacgcggat ggcggtgcgc acaccgcgcg
     1441 agaccgcgcg catcctgttc gacgaccgga cctcgggcct gaacgtcggc ctgctgttcg
     1501 gcggcgagag ggcggggctg gagacgtctg acatcgccct gacccaaggc atcgtcacca
     1561 tcccgatcga cccgcggcac cattcgctga acctcgccca agccgtggcg atcaacgcct
     1621 atgagtggcg aacgacggtg ctggacgccc cgccgccgcg tttccgcgag ggcgacccgc
     1681 cggcgtcggg cgcgctgatg ctggggatgt acgaacatct ggagcgggag ctggacgacg
     1741 gcggcttctt ccatccgccc gagaagcggc cgtcgatggt ccagaacctg cgcgtgatgc
     1801 tgggccggtc ggccttcagc gagcaggagg tggcgacgtt ccggggggtg atcacggccc
     1861 tgtccaaggg gcggggccgg gtgctggcga agctggcggc gaagaaggcg gcgggcgagg
     1921 gctaggccag cagattgtgc acgaaggccg ggaccatctg cgtcgccggg ccgtagacgc
     1981 cctcgtcgaa cagccggcgg ccgggcgttg gctcgaggtt gacctccacc gtgcgggccc
     2041 cggcgaggcc ggcct
//
LOCUS       DASVVN010000516         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_72378, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000516 DASVVN010000000
VERSION     DASVVN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72378"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF686_08375"
     CDS             complement(<1..489)
                     /locus_tag="VF686_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8661869.1"
                     /translation="MALNSVNTNVGAMIALQNLQSTNGELSTVQNRISTGKSVSSAKD
                     NGAIWAIAQGQRAEIGALNAVKDSLNRGQSAVDVSLAAGESVSDLLIQLKEKALAATD
                     KSLTTSARSNLNEDFKAIRDQIATVVTNAKFNGVNLLDNSTTTNGFKALSNTAGSTIK
                     VAG"
     gene            complement(938..1399)
                     /locus_tag="VF686_08380"
     CDS             complement(938..1399)
                     /locus_tag="VF686_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661870.1"
                     /translation="MRPFDLFRALTPLGRGLAAALVFVAALALGHGLGLRWDPFGLAE
                     RRLAAAHALAATAEADAAARRLEVEGAADQAHRLDTHHQQAASVAVATVRAETDARSA
                     HDASIPLDPARAARLREHDRRLCDLAPAVCGPAPADPARGGDDAVPAGSAA"
     gene            complement(1396..1779)
                     /locus_tag="VF686_08385"
     CDS             complement(1396..1779)
                     /locus_tag="VF686_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8661871.1"
                     /translation="MSFRLSERSLKRLEGVHPRLVALVREASALTPVDFMVTEGLRTP
                     ERQAALVRAGASRTRKSRHLTGHAVDVAALVDGQVRWDWPLYPRIAAAFKAAAARQGT
                     AIVWGGDWISLRDGPHFELDRKVFP"
     gene            complement(1776..2039)
                     /locus_tag="VF686_08390"
     CDS             complement(1776..2039)
                     /locus_tag="VF686_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661872.1"
                     /translation="MTDRTDEERPSPLAESRWLWRRLYVFATSGGAWLLLDRLIAAVP
                     PEAAPRLAEGLMTLLALTMVLYLVAPSAQQLIETLGSLRGRRP"
     gene            complement(2036..2203)
                     /locus_tag="VF686_08395"
     CDS             complement(2036..2203)
                     /locus_tag="VF686_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661873.1"
                     /translation="MTRRLTTHDDGSAPALLDAETWDAPARLTDDDLFTQGGDGWRRA
                     AFDLEDPEDFR"
     gene            complement(2200..>2419)
                     /locus_tag="VF686_08400"
     CDS             complement(2200..>2419)
                     /locus_tag="VF686_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661874.1"
                     /translation="GAGAVGGTAPQPGLHWAGGGGGGGGAVASGTGHEGGAGGGAGAG
                     GGGGGAGVVAGGAGGAGAAGLVWIVALG"
BASE COUNT          357 a          858 c          855 g          349 t
ORIGIN      
        1 acccgccacc ttgatggtcg aaccggcagt gttggacagg gccttgaagc cattggtcgt
       61 ggtcgagttg tcgagcaggt tcacgccgtt gaacttggcg ttcgtgacga cggtggcgat
      121 ttggtcgcgg atcgccttga agtcttcgtt caggttggaa cgggccgaag tcgtcaggct
      181 cttgtcggta gcggcaaggg ccttttcctt cagctggatg agcaggtcgg agaccgattc
      241 acccgcggcc aggctgacgt ccacggccga ctggccgcgg ttcaggctgt ccttgacggc
      301 gttcagggcg ccgatttctg cgcgctgacc ctgggcgata gcccagatgg cgccgttgtc
      361 cttggcggac gagacgctct tgccagtgct gatgcggttt tgaaccgtgg acagttcacc
      421 gttggtggac tgaaggttct gaagggcgat cattgcgccc acgttcgtgt taacgctgtt
      481 cagcgccatg acgagtatcc ttgtttcaag gtgtccgacg ccattttggc ggccgggagc
      541 attttgctcg cagctatcgc agcaaggacg ggaccactcg cggacccgac atgccagagc
      601 gccaggcgtt gattttgcta cgttagttct cgttaagcac gtccggcgcc cggcaggatg
      661 tgccgggcgc ccggcaggaa atgcccacgg ggtcggcagc atctgccgtc ggaatggcgc
      721 ggtttgcgga aaacggggcg ccaaccctgc caaaccgcgc cacgcccgtc accggagcgg
      781 ccacggccgg cggcccggcg gcggctcgac ccacctgtcc agcagggcgc gctcggccag
      841 caaggtgtcg accgccaggc tgcgcgacga atcgcacaac agcagggccg caccccgggc
      901 ggcgtaggac gcctccagat cggccagggt cgccccctca ggcagccgat ccagccggca
      961 cggcgtcgtc gccgcctcgg gcagggtcag ccggggcggg gccgcagacg gcgggggcaa
     1021 ggtcgcacag ccggcgatca tgctcgcgca gacgagcggc gcgagcgggg tcgagcggga
     1081 tagaggcgtc atgggcgctc cttgcgtcgg tttcggcgcg gacggtcgcc acggcgaccg
     1141 aggcggcctg ttgatgatgg gtatcgaggc ggtgcgcctg atcggcggcg ccctcgactt
     1201 cgagccggcg cgcggcggcg tcggcttcgg ccgtcgcggc cagggcgtgg gcggcggcca
     1261 gcctgcgctc ggccagacca aacggatccc agcgcagccc cagcccatga cccagcgcca
     1321 gtgcggcgac gaacacgagg gcggcggcca gaccccggcc cagcggggtc agggcgcgga
     1381 acaggtcgaa cggcctcacg ggaacacctt gcggtccagc tcgaaatgcg ggccgtcgcg
     1441 cagggagatc cagtcgccgc cccacacgat ggccgtgccc tgccgggcgg ccgccgcctt
     1501 gaaggccgcg gcgatgcggg ggtacagcgg ccagtcccag cgcacctgcc cgtcgaccaa
     1561 ggcggccaca tcgaccgcat gcccggtcag atgacgggat ttgcgggtcc ggctggcccc
     1621 cgcccgcaca agcgccgcct gacgctccgg cgtgcgcagt ccttcggtga ccatgaagtc
     1681 cacgggcgtc agggccgagg cctccctgac cagagcgacc agccggggat gcacgccctc
     1741 gagtcgtttg agcgatcgtt cggacaggcg gaagctcatg gcctgcgccc ccgcagggaa
     1801 cccagcgtct cgatcagctg ctgggccgac ggcgccacga ggtagagcac catggtcagc
     1861 gccagcagcg tcatcagtcc ctcagccagc cggggcgccg cctcgggcgg tacagcggcg
     1921 atcagccggt ccagcagcag ccaggcgccg ccgctggtgg cgaacacata gagcctgcgc
     1981 cacagccagc ggctctcggc cagcggcgaa ggccgctctt catcagtccg atcggtcatc
     2041 tgaaatcctc cgggtcttca aggtcaaagg cggcgcgacg ccagccgtcg ccgccctggg
     2101 tgaacaggtc gtcgtcggtc agccgggcgg gcgcgtccca tgtctccgca tccagcagtg
     2161 caggggcgct cccgtcgtca tgggttgtca ggcggcgggt catccgaggg ccacgatcca
     2221 gaccaggccg gccgcgcccg cgccgccggc gcccccagcg actacgccgg ctcctccgcc
     2281 gccgccgccc gcgcccgctc ctccgcccgc cccgccctca tggccggtcc ctgaggcgac
     2341 ggcgcccccg ccgcctcccc cgccgccggc ccagtgcagg ccgggctgcg gcgccgtccc
     2401 gccgaccgcc ccggcgccc
//
LOCUS       DASVVN010000517         3943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_202940,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000517 DASVVN010000000
VERSION     DASVVN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202940"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VF686_08405"
     CDS             complement(<1..1144)
                     /locus_tag="VF686_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8661875.1"
                     /translation="MKKPNSQALSLRVPEPSGRPGDPTDFSHLLMDPAGVVERPEVST
                     TPFDMRDHAFRLIRVLDDDGKAVGPWNPKLDADTLRRGLKAMILTRAFDDRMHRAHRQ
                     GKTSFYMKCTGEEAIAVAQGMILSREDMGFPTYRQQGLLIARGYPLATMMNQIYSNAS
                     DPIKGRQLPIMYSAKDYGFFTISGNLGTQVPQAVGWAMASAYKGDDKIAITWIGDGAT
                     AEGDFHNALTFASVYRAPVILNIVNNQWAISSFQGIAGGLETTFASKAIGYGLPALRV
                     DGNDFLAVWAATQWAEERARSNQGATVIELFTYRGAPHSTSDDPSRYRPADEHEKWPL
                     GDPVARLKQHLIALGEWSDDQQTAAEADAVEQVRAAGKESEAIGTLG"
     gene            1289..1777
                     /locus_tag="VF686_08410"
     CDS             1289..1777
                     /locus_tag="VF686_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8661876.1"
                     /translation="MADDLDPVDARILDILQQDAGLSVAEVADRVGLSASPCWRRIKR
                     LEDTGFIRKRVTLLDATKLGLDFEVYAIVKLSLPSTENLDAFESAVAAWPEVVQCATI
                     TGREDYVLRIITSDMHAFDQFLRHKLLSLGIVSDCESHIVTRGVKNVTALPLGIVTPH
                     VG"
     gene            1803..2780
                     /locus_tag="VF686_08415"
     CDS             1803..2780
                     /locus_tag="VF686_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661877.1"
                     /translation="MRTLAKVTGLALALMAGAASAQSLETLVATAAEPVTIVRVARGA
                     DGSLILSSVWLGRLYRAEADGRLTTLLEADPEMGFYGLAADPDRGVLYAVSAERPSRT
                     GAKGTSLLKIDLATGAVLDRATPEAPAHRFGDVAVGPDGSAYVADLRGVRIFVWRPGQ
                     ALATLAVLPERASPQGMAVSPNGRWLILADYRTGLHRIDLAAAAQGPLTLTAESYQPL
                     TPPDAGELRGIDGLARRGDTILAMQNGTQTHRVLRVTMNPDWSAITGRETLHEGAPLD
                     DPTTGFVEGDSLIFVSRSQWSDFNLDGAKTPTPAPAVVSRLPLIPETRP"
     gene            2777..3709
                     /locus_tag="VF686_08420"
     CDS             2777..3709
                     /locus_tag="VF686_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8661878.1"
                     /translation="MIEMVIDARRKDLGGFEVGRVLPFHARRMVGPFIFLDQMGPAEF
                     APGSEAINVRPHPHIGLSTLSYLFEGEIMHRDNTGATQAIRPGEVNWMTAGKGIVHSE
                     RTDPLKRSTGGPMHGMQAWIALPEEAEETDPRFHHHGEADQPAYENQGLFARLVAGEA
                     YGAKAGVPVSSPLFYVHWELAEGVRTAPPPGRGAGGYSERALYVAKGEIEVGDRVFHE
                     GQMIVLEPAAEPTIKALRPSTVMVLGGEPVGPRIIWWNFVHSKQERIDQAKADWKAGR
                     MALPSEDSIEFIPLPDVPSTPEPAPVKPDQTHPV"
     gene            complement(3716..>3943)
                     /locus_tag="VF686_08425"
     CDS             complement(3716..>3943)
                     /locus_tag="VF686_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8661879.1"
                     /translation="LAVYGGKDVQVPADQNGPAIARLKPDAEVIVLPGLNHLFQPAQT
                     GLMAEYGQIETTLDPSVIQTVVDWIAARSGL"
BASE COUNT          611 a         1276 c         1402 g          654 t
ORIGIN      
        1 ggcccagcgt gccgatggcc tcggactcct tgccggcggc gcggacctgt tcgacggcgt
       61 cggcctccgc ggccgtctgt tggtcgtcgg accattcgcc gagcgcgatg aggtgctgtt
      121 tcaggcgggc gacgggatcg cccaacggcc atttttcgtg ctcgtcggcc ggacggtagc
      181 ggctggggtc gtccgaggtc gagtgcggcg cgccgcggta ggtgaacagc tcgatgaccg
      241 tcgcaccctg gttggagcgg gcgcgctcct cggcccactg ggtggccgcc cagacggcga
      301 ggaagtcgtt gccgtcgacg cgcagggccg gaaggccgta gccgatggcc ttggaggcga
      361 aggtggtctc cagcccgccg gcgataccct ggaaggagct gatggcccac tggttgttga
      421 cgatgttgag gatgacaggc gcgcggtaga cgctggcgaa ggtcagggcg ttgtggaagt
      481 cgccctcggc cgtggcgccg tcgccgatcc aggtgatggc gatcttgtcg tcgcccttgt
      541 aggcgctggc catggcccag ccgacggcct gcggcacctg ggtgccgaga ttgccgctga
      601 tggtgaagaa gccgtagtcc ttcgccgaat acatgatcgg cagctgacgg cccttgatcg
      661 ggtccgaagc atttgagtag atctggttca tcatggtcgc gagcggatat ccgcgcgcga
      721 tcaacagacc ctgctggcga taggtgggga agcccatgtc ctcacgcgac aggatcatgc
      781 cctgggcgac ggcgatcgcc tcctcgcccg tgcatttcat gtagaagctg gtcttgccct
      841 gacggtgggc gcgatgcatc cggtcgtcga aggcgcgggt caggatcatg gccttgaggc
      901 cgcggcgcag ggtgtcggcg tccagcttgg ggttccacgg gccgacggcc ttgccgtcgt
      961 cgtccagcac gcggatcagc cggaaggcgt gatcgcgcat atcgaacggc gtggtcgaca
     1021 cctcgggacg ctccacgacg cccgccggat ccatcagcag atgactgaaa tccgtggggt
     1081 cgccgggccg gcccgaaggc tccggcacgc gcaaagacaa ggcttggcta ttgggcttct
     1141 tcattccgtc ctgccgtccg gttgatccga ccgcgtttcc tgcagcgcaa ttagcacgag
     1201 gcgtttggca aggctcgctc gattttgacc ttgccttacc ccgtttggcg gtattttatt
     1261 gcgccataac aagatcagga gaaacgcctt ggctgacgat ctggatcccg tcgacgcccg
     1321 aattctggac atcctgcagc aggacgccgg attgtccgtg gccgaggtgg cggaccgggt
     1381 cggtctgtcg gcctcgccgt gctggcgccg gatcaagaga ctggaggata cgggcttcat
     1441 ccgcaagcgg gtgaccctgc tggacgcgac caagctgggc ctggatttcg aggtctacgc
     1501 catcgtcaag ctgagcctgc cgtcgaccga aaacctcgac gccttcgagt ccgccgtggc
     1561 cgcttggccc gaggtggtgc agtgcgccac catcaccgga cgcgaggact atgtgctgcg
     1621 gatcatcacc tccgacatgc acgccttcga ccagttcctg cgccacaagc tgctgagcct
     1681 gggcatcgtc tcggactgcg agagccatat cgtcacgcgc ggcgtgaaga atgtgaccgc
     1741 cctgcccctt ggcatcgtga cccctcacgt cggatagtcg ggcggcaaac ggggcgacgg
     1801 gcatgcggac tctggcgaag gtgacgggtc tggcgctggc cctgatggcg ggagcggcgt
     1861 ccgcgcagtc gctggaaacc cttgtcgcca ccgccgcgga gcccgtgacg atcgtgaggg
     1921 tggcgcgcgg cgccgacggt tccctgatcc tctcgtccgt gtggctgggc aggctgtacc
     1981 gggccgaggc cgacggccgg ctgaccaccc tgctcgaagc ggacccggag atgggattct
     2041 acggcctggc cgccgatccg gaccggggcg tgctgtacgc cgtgtcggcg gagcgtccga
     2101 gccggaccgg agccaaggga acctcgctgc tgaagatcga tctggcgacc ggcgcggtcc
     2161 ttgaccgggc gacgccggag gcccccgcgc acagattcgg cgacgtggcg gtcgggcccg
     2221 acgggtcggc ctatgtcgca gacctgcgcg gcgtgcggat cttcgtctgg cgaccggggc
     2281 aggccttggc gacgctcgcc gttctgccgg aacgcgcatc cccgcagggc atggcggtgt
     2341 ctccgaacgg gcggtggctg attctggctg actatcgcac cggtctgcac cggatcgatc
     2401 tggcggcggc cgcccaaggg ccgctcaccc tgacggcaga gagctatcag cccctgacgc
     2461 cgcccgacgc gggagaattg cgcgggatcg acgggctggc gcggcgaggc gacacaatcc
     2521 tggccatgca gaacgggacg cagacccatc gggtgctgcg cgtgacgatg aaccccgact
     2581 ggtcggcgat cacggggcgc gaaaccttgc acgagggggc gccgctggat gacccgacca
     2641 cgggcttcgt cgagggcgac agcctgatct tcgtgtcgcg cagccagtgg tccgacttca
     2701 acctcgacgg cgccaagacc ccgaccccgg cccccgccgt ggtcagccgc ctgcccctca
     2761 ttccggagac ccgaccatga tcgagatggt gatcgacgcc cgccgcaagg atctgggagg
     2821 gttcgaggtc ggccgcgtcc tgccgttcca tgcgcggcgg atggtcgggc ccttcatctt
     2881 tctggaccag atggggccgg ccgagttcgc gcccggatcg gaggccatca atgtgcggcc
     2941 gcatccgcac atcggcctgt cgaccctgag ctatctgttc gagggcgaga tcatgcaccg
     3001 ggacaacacc ggcgcgaccc aggccatccg gcccggcgag gtcaactgga tgaccgccgg
     3061 caagggcatc gtccactccg agcgcaccga tccgctcaag agatcgacgg gcgggccgat
     3121 gcacggcatg caggcctgga tcgccctgcc ggaggaggcc gaggagaccg atccgcggtt
     3181 ccaccaccat ggcgaggcgg accagccggc ctatgagaac cagggcctgt tcgcgcggct
     3241 ggtggcgggc gaggcctatg gcgccaaggc cggcgtgccg gtgtcgtcgc cgctgttcta
     3301 tgtccattgg gaactggccg agggggtgcg cacggcgccg cctccgggcc ggggcgcggg
     3361 cggctattcc gagcgggcgc tgtatgtggc caagggcgag atcgaggtcg gcgaccgtgt
     3421 cttccacgag ggccagatga tcgttctgga gccggcggcc gagccgacga tcaaggcctt
     3481 gaggccctcg accgtcatgg tgctgggcgg agagccggtc gggccgcgca tcatctggtg
     3541 gaatttcgtc cactcgaaac aggaacggat cgatcaggcc aaggccgact ggaaggccgg
     3601 acgcatggcc ctgccgagcg aggactcgat cgagttcatc cccctgcccg acgtgccctc
     3661 gacccccgag ccggcgccgg tcaagccgga ccagacgcat ccggtgtgag gacgactaca
     3721 gccccgagcg ggccgcgatc cagtcgacga cggtctggat gacggatgga tcgagcgtgg
     3781 tctcgatctg accgtactcg gccatcagtc cggtttgagc cggctggaac aggtggttca
     3841 gacccggcag gacgatcacc tcggcgtccg gcttcagccg tgcgatcgcg ggtccgttct
     3901 ggtcggctgg cacctgcacg tccttgccgc cgtagacggc cag
//
LOCUS       DASVVN010000518         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_493141,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000518 DASVVN010000000
VERSION     DASVVN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493141"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF686_08430"
     CDS             <1..326
                     /locus_tag="VF686_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661880.1"
                     /translation="TPGGVIVPLNGWDQGPTTVVFGQPGDTRGTGQIVNTGSAQMQRT
                     QGQNGPVRAGQVQWQQDTLNLGPICISFQGNGQSQSGDVGMSGTTMCVMDAACQQTSG
                     CGTIR"
     gene            323..1582
                     /locus_tag="VF686_08435"
     CDS             323..1582
                     /locus_tag="VF686_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8661881.1"
                     /translation="MTSGVQAFDRLAAALQEIRPRFGAGAFADRRRVIGLLADKVPDA
                     KREIRVVGTAIDEGIPAALARTERHLIGVEMDRLANGLEASTGLRLDIARQVTRAFAF
                     AMDLGPLPSIYEAAGTPAPVAPPPAPVAPVAGGWSGMTAPVQPGPPSMPISQYPPHSQ
                     PTQPYQPGGTAPLGFTVGVPAKKPNMAGRIALGVGGVIVLAIGVSQMMGGGGRPQPGN
                     NVIASSGETGFASELVDQGIAPQAELNANLGSPTPMSIAVGQRVSTVELQRMIQNDPQ
                     LVLVDVLAQPHEQTIQGSYWLPSGGSAGSFQDSYQSDFVAGLNQVTGGVTSRPVVFFC
                     AGANCWESYNATLRANAAGYTRLFWYRGGLASWAEARGPMAALPTQGAAVPTNPQQPQ
                     DQPPVDPNNPPIEQDPNVPPGDKVPTE"
BASE COUNT          263 a          607 c          577 g          225 t
ORIGIN      
        1 cgacgccggg cggcgtgatc gtgccactga acggctggga tcaggggccg acgacggtgg
       61 tcttcggcca gcccggcgac acccgaggga cgggccagat cgtcaacacg ggcagcgccc
      121 agatgcagcg gacacagggc cagaacggcc ccgtccgggc cggccaggtg cagtggcagc
      181 aggacaccct caacctcggg cccatctgca tttccttcca gggcaatggg cagagccaga
      241 gcggcgatgt cggcatgtcc ggcacgacga tgtgcgtgat ggacgcggcc tgccagcaaa
      301 cctcagggtg cggcacgatt agatgacgag cggcgttcag gccttcgatc gactcgccgc
      361 ggcgctgcag gaaatccggc cgcgcttcgg cgcgggcgcg ttcgccgacc gccgccgggt
      421 gatcggcctg ctcgccgaca aggtgccgga cgccaagcgc gagatccggg tggtcggaac
      481 cgcgatcgac gaaggcattc cggccgcgct ggcccgcacc gagcgccacc tgatcggcgt
      541 ggagatggac cggctggcga acggtctgga ggcgtccacg ggcctgcgcc tcgatatcgc
      601 ccgccaggtg acccgcgcct tcgccttcgc gatggacctg gggccgctgc cgtcgatcta
      661 cgaggcggcg ggcacaccgg cgcccgtggc gcccccgccc gccccggtcg cgcccgtcgc
      721 gggcggctgg agcggcatga cggcgccggt ccagcccgga ccgccgagca tgcccatctc
      781 gcaatatccg cctcattcgc agcccaccca gccctatcag ccgggcggca ccgcgccgct
      841 gggcttcacc gtcggcgtcc cggccaagaa gcccaatatg gccgggcgga tcgcgctcgg
      901 cgtcggcggc gtgatcgtgc tggcgatcgg cgtctcgcag atgatggggg gcggcggccg
      961 cccgcagccg ggcaacaatg tgatcgccag ctccggcgag accggcttcg cgagcgaact
     1021 ggtcgatcag ggcatcgccc cgcaggcgga gctcaacgcc aatctcggct cgccgacgcc
     1081 gatgtcgatc gcggtgggcc agcgggtctc cacggtcgag ctccagcgga tgatccagaa
     1141 cgatccgcaa ctcgtcctcg tcgatgtcct cgcccagccg cacgagcaga ccatccaggg
     1201 ctcttactgg ctgccctcgg gcggctcggc cgggtccttc caggacagct atcagagcga
     1261 tttcgtcgcc ggcctcaacc aggtgactgg cggagtcacg tcgcgccccg tcgtgttctt
     1321 ctgcgccggc gcgaattgct gggaatccta caacgccacg ctgcgcgcca acgccgccgg
     1381 ctatacccgc ttgttctggt accgcggcgg gctcgcctca tgggccgagg cgcgcgggcc
     1441 gatggcggcg ctgcccaccc agggcgccgc ggtgccgacc aacccgcagc aaccgcagga
     1501 tcagcccccg gtcgatccca acaatccgcc gatcgagcaa gacccgaatg tgccgcccgg
     1561 cgacaaggtt ccgaccgagt agctacagaa ggtaggtcat ccgcacgatc agagtcgtcc
     1621 ggccctgggc gaccgggaag cgcacgtcct cataacgggg cgggcccatc cg
//
LOCUS       DASVVN010000519         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_365873,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000519 DASVVN010000000
VERSION     DASVVN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365873"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VF686_08440"
     CDS             complement(<1..1669)
                     /locus_tag="VF686_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX8661882.1"
                     /translation="MTDTPASAPVSGGQSTGKRIELTLRALVLGCILAVIFTAANTYL
                     GLKVGLTFASAIPAAVISMAILRMFKTSTIWENMTVQTVASVGGAMSSIIFVLPGLVM
                     VGWWLEFPFWESVLICVFGGILGVTFSIPLRRALVVEAGLPYPEGVAAAEVLTVGSRG
                     AEQTESAVRENAAGLWVVITGAIVSAGYALLVAGRVFAGEAARFVKLPAALGGGATGI
                     GFGMQFALLGAGHLIGLSVGLAQLFGLILAWCIAVPILTSPETIAWLTAHGIPSIATT
                     LPAGVGAEELASTVWRREVRFMGAGVIGVAAIWTLIKLAGPLVGGLTSALAANRRRQS
                     GEVLDRTEQDIPINIVAGLSVACLIGIGFILAWFANGNPTLASSTALLVGGGLIYVVF
                     IGFAVAAICGYMAGLIGSSNSPVSGVGILAIVIASVLMLGVLALAGLPADPSVVAFAL
                     IVTAVVFAVAVIANDNLQDLKTGQLVQATPWRQQTALIVGVIAGAIVIPLILNLLNQA
                     FGFEGGPPAIAQGAQTLAAPQATLISALARGVIEGDLRWDLIGVGAVI"
BASE COUNT          309 a          598 c          576 g          216 t
ORIGIN      
        1 cgatcaccgc gccgaccccg atcaggtccc agcgcaggtc accctcgatc acaccccgcg
       61 ccagcgcgct gatcagggtc gcctgcggcg ccgcgagcgt ctgcgcgcct tgggcgatgg
      121 ccggcggccc gccctcgaag ccgaaggcct ggttgagcag attcaggatc agcggaatga
      181 cgatggcgcc ggcgatgacg ccgacgatca gcgccgtctg ctgccgccac ggcgtggcct
      241 gcaccagctg gccggtcttc aggtcctgca ggttgtcatt ggcgatgacg gccaccgcga
      301 agacgacggc ggtcacgatc agggcaaaag ccacgaccga aggatcggcg ggcaggcccg
      361 ccagcgccag cacgcccagc atcagcaccg aggcgatgac gatggcgaga atgcccacgc
      421 ccgacaccgg gctgttcgac gaaccgatca ggccggccat gtatccgcag atggccgcca
      481 cggcgaagcc gatgaagacg acgtagatca gaccaccccc gaccagcagg gcggtcgagc
      541 tcgccagcgt cggattgccg ttggcgaacc atgcgaggat gaagccgatg ccgatcaggc
      601 aggcgaccga caggccggcc acgatgttga tcggaatgtc ctgctcggtg cggtccagca
      661 cctcgccgct ctgacggcgg cggttggccg ccagcgccga ggtcaggccg ccgaccagcg
      721 gtccggccag cttgatcaag gtccagattg ccgcgacgcc gataacgccg gcgcccatga
      781 accgcacctc ccggcgccag accgtgctgg ccagttcctc ggctcccacc cctgcgggca
      841 gggtcgtggc gatcgagggg atgccgtggg cggtcagcca ggcgatggtc tccgggctgg
      901 tcaggatcgg cacggcgatg caccaggcga ggatcaggcc gaacagctgg gccagaccca
      961 cgctcaggcc gatcaggtga cccgcgccca gcagggcgaa ctgcatgccg aagccgatgc
     1021 cggtcgctcc gccgcccagc gccgccggca gcttgacgaa ccgcgcggcc tcgcccgcga
     1081 acacccggcc ggccaccagc agggcgtagc ccgcggagac gatcgcgccg gtgatcacga
     1141 cccacaggcc ggcggcgttc tcgcgcacgg cgctctcggt ctgttcggcc ccgcgcgagc
     1201 cgacggtcag aacctcggcc gccgcgacgc cttcgggata gggcaggccc gcttccacaa
     1261 cgagcgcgcg gcgcagcgga atggagaagg tgacgcccag aatgccgccg aagacgcaga
     1321 tcagcaccga ctcccagaac gggaactcca gccaccatcc gaccatgacc agaccgggca
     1381 ggacgaagat gatcgagctc atcgctccgc cgaccgaggc gacggtctgg acggtcatgt
     1441 tctcccagat ggtcgaagtc ttgaacatcc gcaggatggc catcgagatg accgccgccg
     1501 gaatggccga ggcgaaggtc agcccgacct tcagacccag ataggtgttc gcggcggtga
     1561 aaatcaccgc caggatgcag cccagaacca gggcgcgcag cgtaagttcg atacgcttgc
     1621 cggtgctttg gccgccgctg acgggcgcgc tcgcgggtgt gtcggtcatg gggttcctct
     1681 ccctcgttgg ccggaccta
//
LOCUS       DASVVN010000520         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_19889, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000520 DASVVN010000000
VERSION     DASVVN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19889"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VF686_08445"
     CDS             <1..964
                     /locus_tag="VF686_08445"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX8661883.1"
                     /translation="SRAFRDWRATCDNTNDCVAFGPASEGTGWVRVGSAPGPNGARTV
                     TVGFWPSSGDGLTGPITLTLDGRRHVAAPIKGDHDPLGAAEVPAAQAAAVASALAAGN
                     TLTLAGAGETVEMSLSGASAAMLWIDERQGRLDTTTALVRKGARPASSVPGPAPAPQV
                     IPAPPIAQTGFGDTGQTLPAALEAVAAVKACRAEIGETAMGDQVMSARLDASTELWAV
                     PCFAGAYNIGHEWYLTGPGGRNPRAVSFASATGETGADTVNGGYSASTRTITAFSKGR
                     GIGDCGTTSTWTWTGSAFVLTAESSMGECWGVPADFWPTTWRTR"
     gene            961..1896
                     /locus_tag="VF686_08450"
     CDS             961..1896
                     /locus_tag="VF686_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8661884.1"
                     /translation="MSDDGPEVLIIGAGPAGLTAATYLARFRRRVLVADGGAPRACWI
                     PLSHNMPGFPSGITGDAILRRMTEQATEFGGIIEGGCVDRLTQDGDGFVARLNGRDLR
                     VRAVLLATGVVDHHPNLPGVERAIERALVRICPICDGYEAIDKAVAVIGDDDKGVREA
                     AFLRTYSDRVTLIHVGPADALTEHGEMARLGIELIRAPIDNVQLEGDRVTALSWGGTF
                     RSFDLVYSALGTSPNADLAETLNARVGDDGRLFVDAHQATSVKGLYAAGDVVRGLNQI
                     AVAGAEAAVAATAIHNALREADGWVVRDQARSRLS"
     gene            complement(2203..4047)
                     /locus_tag="VF686_08455"
     CDS             complement(2203..4047)
                     /locus_tag="VF686_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8661885.1"
                     /translation="MLSRRRLLTSAAGGMGLLALPAIARAGVPKWQGEARSQARALYD
                     AIFEGTLKASPIGATGLGLDVGARAALKTRLDSRSYEDRLNIFKPMVEARPALAAIDA
                     SGLTGRDRSDFDTVVWMADRSAEIAAFPFGGVDSYGYPCPYVITQLTGLYQAVPDFLD
                     SQHSVASAADAEAYLSRLESFAEGMNHETARSASDAADHGIVPPDFVINKALTQLRAL
                     RLETGEGAGLVRSLVRKTAEAGIAGDWARRAAVLVDGPVAAALDAQIAQMERRLPNAS
                     HDAGIGQRPNGEAYYNLCLRFQTSTGMSPREAHQVGLDQVAQIEALADPLLRQRGYTE
                     GSVGARLTAFGRQPEYLYPNTDDGRLALLADLNRQVAAIEARLPDVFERLPRARVEVR
                     RVPPAIELGAPRGYAQSPSLDGSRPGAYYINLVDTGIWPKWALPTLTYHESLPGHHLQ
                     GALALEATDTPLLHKSLGFNAYGEGWGLYAEQLADELGMYDDFTEGKLGYHQSFLYRA
                     ARIVIDTGIHALDWSRERAIAYMIDTVGLAPGAAESEIERYCVWPGQACGYKIGHTEI
                     DRLRTVARERTGERFDIKSFHSAVLDGGAMPLEVLGRVVDQWTAARLR"
BASE COUNT          681 a         1519 c         1436 g          614 t
ORIGIN      
        1 aagccgcgcg ttccgcgact ggcgcgccac ctgcgataac accaacgact gcgtcgcctt
       61 cggcccggcg agcgagggca caggctgggt ccgggtcggc tcggcccccg gacccaacgg
      121 ggcgcgaacc gtcacggtcg gcttctggcc ttcgagcggc gacggcctga ccgggcccat
      181 tacgctgacg ctcgacggcc ggcgccacgt cgcggctccc atcaagggcg atcacgatcc
      241 tttgggcgcg gccgaagtcc ccgccgccca ggcggcggcc gtcgcctccg cacttgccgc
      301 cgggaacacc ctgacgctgg cgggcgccgg cgagaccgtc gagatgtcgc tctccggcgc
      361 ctcggcggcc atgctgtgga tcgacgagcg tcagggacgg ctggacacca ccaccgcgct
      421 cgtccgcaag ggagcgcggc ccgcatcgtc cgtgccgggg cccgccccgg ccccgcaggt
      481 catccccgcc ccgccgatcg cccagaccgg cttcggcgat accggccaga ccctccccgc
      541 agctctggag gccgtggcgg cggtcaaggc gtgccgggcc gagatcggcg aaaccgcgat
      601 gggcgatcaa gtcatgtcgg cgcgcctgga cgcctcgacc gaactctggg ccgtgccgtg
      661 cttcgccggg gcctacaaca tcggccacga atggtatctg accgggccgg gcggacggaa
      721 cccgcgcgcc gtcagctttg cgtccgccac cggcgaaacc ggcgccgaca cagtcaatgg
      781 cggctatagc gccagcacac gcaccatcac cgccttctcc aagggccgcg gcatcggcga
      841 ctgcggcacg acgtcgacct ggacatggac gggcagcgca ttcgtcctga ccgccgagtc
      901 ttccatgggc gagtgctggg gcgtccccgc cgacttctgg cccaccacct ggcggacgcg
      961 atgagcgatg acggccccga agtcctgatc atcggggccg gacccgccgg cctgacggcg
     1021 gccacctatc tggcgcggtt ccggcggcgc gttctggtcg ccgacggggg cgccccgcgg
     1081 gcctgctgga taccgctcag ccacaacatg ccgggctttc ccagcggcat caccggcgac
     1141 gcaatcctga ggcggatgac cgaacaggcc acagaattcg gcgggatcat cgagggcggc
     1201 tgcgtcgacc gcctgacgca ggacggcgac ggcttcgtcg cccggctgaa cggccgcgac
     1261 ctgcgcgttc gtgcggtcct gctggccacc ggcgtcgtgg atcatcaccc gaacctgccc
     1321 ggcgtcgaac gggccatcga gcgggctctg gtccgcatct gcccgatctg cgacggctac
     1381 gaggccatcg acaaggccgt tgcggtcatc ggcgatgacg acaagggcgt gcgcgaggcc
     1441 gccttcctgc gcacctattc cgaccgcgtg accctgatcc atgtggggcc cgccgatgcg
     1501 ctgaccgagc atggggagat ggcgcggctg gggatcgaac tgatccgcgc cccgatcgac
     1561 aatgtccagc tggaaggcga ccgggtcacg gccctgagct ggggcgggac gttccgcagt
     1621 ttcgacctcg tctactcggc gctgggcacg tctcccaacg ccgatctggc ggagacgttg
     1681 aacgcgcgcg tcggcgacga cggccggctg ttcgtcgacg cgcatcaggc gaccagcgtg
     1741 aagggtctct acgccgccgg cgacgtcgtg cgcggcctca accagatcgc cgtcgcgggg
     1801 gccgaggccg ccgtcgccgc caccgccatc cacaacgccc tgcgcgaggc cgacggctgg
     1861 gtcgtgcggg accaggcccg atcgcgactc agctgagctg ttggcagggt tttcaggatt
     1921 cggggcgcca accctgccaa ccctgccaac cggccacgac caaaaagcta gaagagtctg
     1981 gatgttagag cgcctgatgg ctgtcggccc agaccaaaca gcgccagatt atcggaaatt
     2041 tctcgccaga gcgcggctac gtccccacct ccgtcgttcg attttcaaag accgtcaaga
     2101 tcgtcagatt gcgcacctga catggggcag accgggcgaa ggtcaagacg aagacaggaa
     2161 taaattcctg tccaaccttc atctcaaaga acaggattgg tgctagcgca gccgcgccgc
     2221 cgtccactga tcgaccaccc ggcccagcac ctccagcggc atggccccgc cgtccagcac
     2281 cgcagagtgg aagctcttga tgtcgaaccg ctcgccggtg cgctcgcggg ccacggtcct
     2341 caaccggtcg atctcggtgt ggccgatctt gtagccgcag gcctgacccg gccagacgca
     2401 gtagcgctcg atctcgctct cggccgcgcc gggcgccagg ccgaccgtgt cgatcatata
     2461 ggcgatggcc cgctcgcggc tccagtccag cgcgtggatg ccggtgtcga tgacgatgcg
     2521 cgccgcccgg tacaggaacg actggtggta gcccagcttg ccctcggtga agtcgtcgta
     2581 catgcccagc tcgtcggcca gctgttcggc gtaaagaccc cagccctcgc cataggcgtt
     2641 gaagcccagc gacttgtgca gcagcggcgt atccgtcgcc tccagcgcca gcgccccctg
     2701 caggtgatgc ccgggcaggc tctcgtgata ggtcagggtc ggcagggccc acttgggcca
     2761 gatccccgta tcgaccaggt tgatgtagta ggcgcccggc cgcgacccat ccagcgacgg
     2821 gctctgggcg tagccgcgcg gcgcccccag ttcgatggcc ggcggcacgc ggcgcacctc
     2881 gacccgcgcc cgcggcagac gttcgaacac gtccggaagc cgcgcctcga tggcggccac
     2941 ctgccgattc agatccgcca gcagcgccaa ccgcccgtca tctgtgttcg gatagaggta
     3001 ctccggctgt cggccgaagg cggtcagccg cgcgccgacc gagccctcgg tatagccgcg
     3061 ctggcgcagc agcggatcgg ccagcgcctc gatctgcgcc acctgatcga gcccgacctg
     3121 atgcgcctcg cgcgggctca tccccgtcga ggtctggaac cggagacaca ggttgtaata
     3181 ggcctcgccg ttcggtcgct ggccgatccc ggcgtcatgg gacgcgttcg gaaggcgtcg
     3241 ttccatctgg gcgatctggg cgtccagcgc cgccgcgacc ggcccgtcga ccagcaccgc
     3301 cgcccgccgc gcccaatcgc cggcgatccc ggcctcggcc gtcttgcgga cgagggacct
     3361 aacaaggccg gcgccctcgc ctgtttcgag gcgcagggcg cgaagctggg tgagggcctt
     3421 gttgatcacg aaatcgggcg gaacgatccc gtggtcggcc gcgtccgaag ccgagcgcgc
     3481 cgtctcgtgg ttcatgccct cggcgaaact ctccagccgc gacagatagg cctctgcgtc
     3541 agccgccgag gcgacggaat gctggctgtc gaggaaatcg ggaacggcct gatacaggcc
     3601 ggtcagctgg gtgatcacat agggacaggg gtagccgtag ctgtccacgc cgccgaacgg
     3661 gaaggcggcg atctccgccg accggtcggc catccagacc acggtgtcga agtccgaccg
     3721 gtcccggccg gtcagacccg aagcgtcgat agccgccaag gccgggcggg cctcgaccat
     3781 aggtttgaag atgttcagcc ggtcttcata gctgcggctg tcgaggcggg ttttcagcgc
     3841 ggcccgggcg cctacatcca gcccgagacc cgtagccccg atcggcgacg ctttcagcgt
     3901 gccttcgaag atggcgtcat acagggcgcg ggcctgggac ctcgcctcgc cctgccattt
     3961 cggaacaccg gcgcgggcga tggccggaag agcgagaagt cccatgccgc cagcggcgga
     4021 cgtcaacagg cggcggcgac tgagcatgcg tatctcccca acgagggccg caccctcgcc
     4081 cggcggcctc cgcctgacaa ggctcgggga tgttcagcgc cggacacggt tcagcccgga
     4141 ccccgggagc aggagactcc aggatccggg cctcgaccca tcggggggcc gacgatcacg
     4201 ccttggggag aagggaattt cgcctgcggc agaccggatg agcgaaccgg
//
LOCUS       DASVVN010000521         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_335440,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000521 DASVVN010000000
VERSION     DASVVN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335440"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..149
                     /locus_tag="VF686_08460"
     CDS             12..149
                     /locus_tag="VF686_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8661886.1"
                     /translation="MYPRGPQGLPVTERLMQRVISLPMHADLDEATQDRIIAAVASFK
                     K"
     gene            262..1191
                     /locus_tag="VF686_08465"
     CDS             262..1191
                     /locus_tag="VF686_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8661887.1"
                     /translation="MSHPTPLKVGVAGVGVMGRNHARVLSDIRDFDLTAVFDPDAATA
                     EGIAALYEAQAFTTAGDFVAAGLDAAVVATPNRHHAELGVALLEKGVHVLVEKPIAAT
                     IADAQRMIDAARANDRVLMVGQVERFNPAVDAVKRAIDGEDVISIQITRVGPFPPRMG
                     EVGVVIDLAVHDIDIIRYLTESEIVEVQPQLARTKAEREDTALLQFRLANGTIAHITT
                     NWVTPYKVRTLQVATMGKFVVADLITRQVTEYFGQQPDGSYQTRAVNSWPAEPLKKEL
                     EAFAHAIRTGERPAVTGEDGLRNLEVALRCLGE"
     gene            1188..1769
                     /locus_tag="VF686_08470"
     CDS             1188..1769
                     /locus_tag="VF686_08470"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8661888.1"
                     /translation="MKLVPAVLENRFVRLEPFEDHHREPLRIACDADPDLWAALYYNS
                     LGGEEFDGGWDAMRAQQAKGWRIPFAVIHDGDCVGLSTFITPDAKNASVEIGTTYYRP
                     DSRGGVVNPAVKRLMLEHAFACGAGRVAFQVDAINARSRAAMRKLGAVEEGVMRNDKI
                     TWTGRVRSSVIFSILPEEWPDVRARLDERLAVF"
     gene            complement(1766..2248)
                     /locus_tag="VF686_08475"
     CDS             complement(1766..2248)
                     /locus_tag="VF686_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8661889.1"
                     /translation="MSRTERKGTPTPVADLPGLIGQEIGVSRWIRVDQARIDAFAEIT
                     EDRQFIHVDPVAAARTPFGGTIAHGFLTLSLLSAMTYDAVPPLEGVVMGVNYGFDKLR
                     FLAPVPTGSNVRGRFKLLSAEDKGRNRWLLKHEVTVEIEGAEKPALIAEWLGMQVVGG
                     "
     gene            complement(2277..>3001)
                     /locus_tag="VF686_08480"
     CDS             complement(2277..>3001)
                     /locus_tag="VF686_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8661890.1"
                     /translation="CAARMMGTSAIIVMPADSPRVKVDGVIGFGGEVRMYDRYTESRE
                     DIGEEIARTRGSILVRPFDDPFVIEGQGTIGLEIMEDAGTPLDQLVCGASGGGMIAGI
                     NLSMAALSPDTPVIAVEPRHYNDMQLSIAAGERLTHAPAPGTICDALMTDRPGELTFP
                     INRRVDRVETVSDAEVAEAVRYAFHALKLVVEPGGAVSLAAVLSGKVEARDRTTAVIL
                     SGGNVDPALFAAIIEDRFVPIP"
BASE COUNT          487 a         1011 c         1038 g          465 t
ORIGIN      
        1 cggcctatga catgtatccg cgcggaccgc agggcctgcc cgtcacggag cggctgatgc
       61 agcgggtgat cagcctgcct atgcacgccg atctcgacga ggcgacgcag gacaggatca
      121 tcgcggcggt cgcctcgttc aaaaaatgat acgaccgtca ttccggggcc gcgaagcgga
      181 acccggaacc caggaccgcg ccttgaaccc ctgggttccg ggttcgcctg cggcgccctg
      241 gaatgacggc gaggagagag catgagtcat cccacccccc tcaaggtcgg cgtcgccggc
      301 gtcggcgtca tggggcgcaa ccacgcccgc gtcctgtcgg acatccgtga tttcgacctg
      361 accgccgtct tcgatccgga cgccgcgacc gccgagggga tcgcggccct ttatgaggcg
      421 caggccttca ccacggccgg ggacttcgtc gccgccggtc tggacgccgc cgtggtcgcc
      481 acgccgaacc ggcaccacgc tgaactcggc gtcgccctgc tggaaaaggg cgtccacgtg
      541 ctggtggaga agccgatcgc cgccaccatc gccgatgcgc agcggatgat cgatgcggcg
      601 agggcgaacg accgggtgct gatggtcggt caggtcgagc gcttcaaccc ggcggtggat
      661 gcggtcaagc gcgccatcga cggcgaggac gtgatctcga tccagatcac ccgcgtcgga
      721 cccttcccgc cgcgcatggg cgaggtcggg gtggtcatcg atctggcggt gcatgacatc
      781 gacatcatcc gctatctgac agagtccgaa atcgtcgagg tccagcccca gctggcgcgc
      841 accaaggccg agcgcgagga cacggccctg cttcagttcc ggctggccaa cgggaccatc
      901 gcccacatca cgaccaactg ggtcacgccc tacaaggtgc gcacgcttca ggtcgcgacc
      961 atgggcaagt tcgtggtcgc cgacctgatc acccgtcagg ttacggagta tttcggccag
     1021 cagcccgacg gctcgtatca gacccgcgcg gtcaacagct ggcccgccga accgctgaag
     1081 aaggagctgg aggctttcgc ccacgcaatc cgtacgggcg aacggccggc ggtcaccggc
     1141 gaggacggcc tgcggaacct cgaagtggcg ctgaggtgtc tgggggaatg aagctggtcc
     1201 ctgcggtgct ggagaaccgc ttcgtccggc tggagccgtt cgaggatcac cacagggagc
     1261 cgctgcgcat cgcctgcgac gccgatcccg atctgtgggc cgccctctat tacaactcgc
     1321 tcggcggcga ggaattcgac ggcggctggg acgccatgcg cgcgcagcag gccaagggct
     1381 ggcgcatccc gttcgcggtg atccacgacg gcgactgcgt cggactgagc accttcatca
     1441 cgcccgatgc gaagaacgcc tcggtcgaga tcggcacgac ctactaccgg ccggactccc
     1501 gcggcggcgt ggtcaatccg gcggtgaagc ggctgatgct ggagcacgcc ttcgcctgcg
     1561 gcgccggccg cgtcgccttc caggtggacg cgatcaacgc ccgcagccgg gccgccatgc
     1621 gaaaactggg cgcggtcgag gagggcgtga tgcgcaacga caagatcacc tggaccggtc
     1681 gcgtgcgcag ctcggtgatc ttttccatcc tccctgaaga atggccggac gtgcgggcgc
     1741 ggctggatga acggctggcg gtgttctagc cgcccaccac ctgcatcccg agccattccg
     1801 cgatcagggc cggcttttcg gcgccctcga tctcgacggt gacctcgtgt ttcagcagcc
     1861 agcggttgcg ccccttgtcc tccgcgctga gtagtttgaa tcgcccacgc acattggagc
     1921 ccgtcggcac cggggcgagg aagcgcagtt tgtcgaagcc gtagttgacg cccatcacca
     1981 cgccctccag cggcgggacg gcgtcatagg tcatggccga cagcaggctg agggtcagga
     2041 agccgtgggc gatcgtgccg ccgaacgggg tccgggccgc cgccacggga tcgacgtgaa
     2101 tgaactgccg atcctcggtg atttcggcga aggcgtcgat gcgggcctga tccacccgga
     2161 tccagcggct gacgccgatc tcctggccga tcagccccgg caggtctgcg acgggcgtgg
     2221 gcgtaccctt gcgttcggtg cggctcatgg gcgtcaggct cctcaggcga tctggatcag
     2281 gggatgggga cgaagcggtc ctcgatgatg gcggcgaaca gggcggggtc gacgttgccg
     2341 cccgacagga tgacggcggt ggtccggtcc ctcgcctcga ccttgccgga caggacggcg
     2401 gccagcgaca cggccccgcc gggctcgacc acaagtttca gcgcgtggaa ggcgtagcgg
     2461 acggcctcgg ccacctccgc gtcggagacg gtctccacgc ggtcaacgcg gcggttgatc
     2521 gggaaggtca gttcacccgg ccggtcggtc atcagggcgt cgcagatggt gcccggcgcc
     2581 ggggcatggg tcaggcgctc gccggcggcg atcgacagct gcatgtcgtt gtagtggcgc
     2641 ggctcgaccg cgatcaccgg cgtatcgggc gacagggcgg ccatggacag attgatcccg
     2701 gcgatcatgc ccccgcccga ggcgccgcag accagctggt ccagcggcgt tcccgcatcc
     2761 tccatgatct ccaggccgat ggtgccctgg ccctcgatga cgaaggggtc gtcgaacggc
     2821 cggacgagaa tcgagccgcg agtgcgggcg atctcctcgc cgatgtcctc gcggctttcg
     2881 gtgtagcggt cgtacatccg cacctcgccg ccgaagccga tgacgccgtc gaccttcacc
     2941 cgcggactgt cggcgggcat cacgatgatg gcgctggtcc ccatcatccg cgcggcgcag
     3001 g
//
LOCUS       DASVVN010000522         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_493496,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000522 DASVVN010000000
VERSION     DASVVN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493496"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VF686_08485"
     CDS             <1..1525
                     /locus_tag="VF686_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8661891.1"
                     /translation="VLRSPFCDVTDFGDPHSLYPLADKEGREGRSLWRELRLRADEQP
                     QWRRARDFVQAALDARDRDPFAFFSSLLNRIDETGVSGRARILTRLGREAEEAIDETL
                     AQVLAAEGRGGVDLETCVALLEAADVEVKREMEGARNEVRVMTVHGAKGLEAPVVILP
                     DTTSRAKPQGPTLMPSADEDGEEGEGWLMCPGSAKDDCPASKAAREARVARTDAESLR
                     LLYVALTRARDRLVVMGRAVRRPEEGFEAGSWWSVVAETFQRLGDDEPLNARDIGDGV
                     LRFGVDPETLSTGVVARPATAAAPAWARALPAPDHAARFASPSKMEETTRIPAPSPLA
                     TTAGPGAPLGRFRRGDLIHRLLERLPDIAPADRADAADRIMSRERDLDDAARAEMIEA
                     AFRVLDDARFAPVFGPGSRPEVALTGSVGSVPISGRMDRLVITPDRVLVVDYKTNRPA
                     PDRIEDADPAYVLQMAVYVSVLARLYPDRVVEAALVWTDGPRLMPVPAEMMRAALAG"
     gene            1646..1984
                     /gene="trxA"
                     /locus_tag="VF686_08490"
     CDS             1646..1984
                     /gene="trxA"
                     /locus_tag="VF686_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8661892.1"
                     /translation="MATIKVTDDSFDADVLKASQPVLVDFWAEWCGPCKQIGPALEQI
                     ADDLAGQVTIAKVNIDDSPMVPSKLGVKGIPTLMLFKDGQMASMKVGAMPKGKILEWL
                     AEAGIGQAAA"
     gene            2077..2343
                     /locus_tag="VF686_08495"
     CDS             2077..2343
                     /locus_tag="VF686_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661893.1"
                     /translation="MLTAALIAAALSFDGGQVAVIATAPAPAQARQTTDQGPTRRVCG
                     YMRATGSNLQQRVCRNIPISGVQDQQTREFLRNLQRIRFPDESG"
     gene            complement(2357..>2582)
                     /locus_tag="VF686_08500"
     CDS             complement(2357..>2582)
                     /locus_tag="VF686_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX8661894.1"
                     /translation="SGAAVFTVRFDGAAAEPEALAAVARGHGLGATPCASVDEALERA
                     LRLGAGRVAICGSLYLAGEVLGAARETWPD"
BASE COUNT          379 a          949 c          896 g          358 t
ORIGIN      
        1 ggtgctgcgc agccccttct gcgacgtcac cgatttcggc gacccgcaca gcctgtatcc
       61 gctggccgac aaggagggcc gcgagggccg gtcgctgtgg cgcgagctgc ggcttcgggc
      121 cgacgaacag ccgcagtggc gccgcgcgcg cgacttcgtc caggccgcgc tggacgcccg
      181 cgaccgcgat ccgttcgcct tcttctccag cctgctgaac cggatcgacg agacgggcgt
      241 ctcgggccgc gcccgcatcc tgacccggct gggccgcgag gccgaggagg cgatcgacga
      301 gaccctggcc caggttctgg ccgccgaggg ccgcggcggc gtcgatctgg agacctgcgt
      361 cgccctgctg gaagccgccg acgtcgaggt caagcgcgag atggaggggg cccgcaacga
      421 ggtccgcgtc atgaccgtgc acggcgccaa ggggctggag gcgcccgtcg tcatccttcc
      481 cgacaccacc tcgcgcgcca agccgcaggg tccgaccctg atgccgtcgg cggacgagga
      541 cggcgaggag ggggagggct ggctgatgtg ccccggctcg gcgaaggacg attgcccggc
      601 gtcgaaagcc gcgcgcgagg cccgcgtggc ccgcaccgac gccgaatccc tgcgcctgct
      661 ctatgtggcc ctgacccgcg cccgcgaccg gctggtcgtc atgggccggg cggtgcggcg
      721 gcccgaggag ggcttcgagg ccggcagctg gtggagcgtc gtcgccgaaa ccttccagcg
      781 gctgggcgac gacgagcccc tgaacgcgcg cgacatcggc gacggcgtgc tgcgcttcgg
      841 ggtcgacccg gaaaccctct ccaccggggt cgtcgcccgt ccggcgacgg cggccgctcc
      901 cgcctgggcc cgggccttgc ccgctccgga ccacgcggcc cgtttcgcct cgccctcgaa
      961 gatggaggag acgacccgca tccccgcccc ctcgccgctg gcgaccaccg ccgggccggg
     1021 cgcgccgctg ggccggttcc ggcgcggcga cctgatccac cgcctgctgg agcgcctgcc
     1081 cgacatcgcg ccggcggacc gggccgatgc ggcggaccgg ataatgtccc gtgagcggga
     1141 ccttgacgac gccgcgcgcg ccgaaatgat cgaggccgcc ttccgggttc tggacgacgc
     1201 ccggttcgcg ccggtgttcg gtcccgggtc ccgccccgaa gtcgccctga ccggatcggt
     1261 cgggagtgtc ccgatctcgg gacgcatgga ccggctggtg atcacgcccg accgtgtgct
     1321 ggtcgtcgac tacaagacca accgcccggc cccggaccgg atcgaggacg ccgacccggc
     1381 ctatgtgctg cagatggcgg tctacgtctc ggtcctggcc cgactctatc ccgaccgtgt
     1441 ggtcgaggcg gcgctggtct ggaccgacgg accgcggctg atgcccgtgc ccgccgaaat
     1501 gatgcgagca gcgctggccg gctgaccgct gtggcgttga tttgcagcgc ggaacatccc
     1561 acatcctcgg caacgggccg gaatcagata gtctgacggc cgatcatttg cgtcgccccg
     1621 ggcggcgcca gacaggaata tccgaatggc caccatcaag gtcaccgacg acagcttcga
     1681 cgccgacgtt ctcaaggcgt cccagcccgt gctggtcgac ttctgggccg aatggtgcgg
     1741 cccgtgcaaa cagatcgggc cggctctgga gcagatcgcc gacgatctgg ccggtcaggt
     1801 caccatcgcc aaggtcaata tcgacgacag ccccatggtc ccgtcgaaac tgggcgtgaa
     1861 gggcattccg accctgatgc tgttcaagga cggccagatg gcctcgatga aagtcggcgc
     1921 catgcccaag ggcaagattc tggaatggct ggctgaagcc ggcatcggac aggccgccgc
     1981 ctgatgggac cggggtcgaa cggccccgga tttgccctcg ccgacgatcg gtgttctctt
     2041 gcgtcttcaa ccgcaggatc gccggagcct tacgccatgc tgaccgccgc cctcatcgcc
     2101 gccgccctct cgttcgacgg agggcaggtc gctgtcatcg ccacggcccc cgcgcccgcc
     2161 caggcgcgtc aaaccacgga tcaggggccg acgcgccggg tctgcggcta catgcgggcg
     2221 accggatcga acctgcagca gcgcgtctgc cgcaacattc ccatctcagg cgtacaggat
     2281 cagcagacgc gcgaattcct gcgcaacctg cagcgcatcc gtttcccgga cgaaagcggc
     2341 tgacggcggc cgtctcctag tcgggccagg tctcgcgcgc cgcgccgagc acctctcccg
     2401 ccagatacag cgaaccgcag atcgccaccc ggcccgcgcc cagccgcagc gcccgctcga
     2461 gcgcctcatc gaccgaggcg cagggcgtcg cgccgaggcc atgtccgcgc gcgacggcgg
     2521 ccagggcttc cggctcggcc gccgcgccgt cgaagcgcac ggtgaagacg gcggctcctg
     2581 ac
//
LOCUS       DASVVN010000523         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_493512,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000523 DASVVN010000000
VERSION     DASVVN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493512"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VF686_08505"
     CDS             <1..1093
                     /locus_tag="VF686_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8661895.1"
                     /translation="KYLVKEDGTLGERNGFRAVGVEHKLGIHASPTCVMSYEGATAEL
                     VGQPNQGLAHMFVMMNAARLAVGVEGVGIAERAYQHALTYARERRQGRSVWTGEANAP
                     IVDHPDVRRTLSVMKAKVAAARAICLSTGVAADLAKHSATEEERRRWKGREDLFTPIA
                     KAWSTDVGVEVASLGVQIHGGMGFIEETGAAQHYRDARIAPIYEGTNGIQAMDLVGRK
                     LSMDGGQSARDLIADMKLTLAQLPALYSGKPVERFAAAISAVEDATLWLLDRKAAADG
                     GADVLAAADAYLKLLGDVAGGWMLADGALYARDQLDGGQGDPAWLEGKITLYEVYAAN
                     VLGHASSRLAAVGQGGALLERMSVDVLAG"
     gene            complement(1101..>1945)
                     /locus_tag="VF686_08510"
     CDS             complement(1101..>1945)
                     /locus_tag="VF686_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661896.1"
                     /translation="IGVEFGSFYRALGAEVTVVEAVDRIMPVEDEEVSKAAQKAFEKR
                     GMKFRTGCKVTKVSKGGKGVQVAIEAGGKAETLEAEVCISAVGITANTDGIGLEALGV
                     NMDRGHITIDGHCQTNVKGLYAIGDCAGAPWLAHKASHEGIHAAEYIAGYKTPNVNSP
                     IAGCTYAQPQVASVGITEQAAREAKREVKVGRFPFRVNGKAVAAGETEGFVKVIFDAK
                     TGALIGAHMIGAEVTEMIQGYVTAIAMEATEEDIHGIVYPHPTMSEAMHEAALDAYGR
                     VIHI"
BASE COUNT          290 a          676 c          662 g          317 t
ORIGIN      
        1 caaatacctc gtgaaggagg acggcacgct cggcgaacgc aacggcttcc gggccgtggg
       61 ggtcgagcac aagctgggca tccacgcctc gccgacctgc gtcatgtcct atgaaggcgc
      121 gaccgccgaa ctggtcggac agcccaatca gggcctggcc catatgttcg tgatgatgaa
      181 cgccgcccgc ctcgccgtgg gcgtcgaggg cgtcggcatc gccgagcgcg cctatcagca
      241 cgccctcacc tacgcccgcg agcggcgcca gggccggtcg gtctggaccg gcgaagccaa
      301 cgccccgatc gtcgatcatc ccgacgtgcg tcgcaccctt tcagtgatga aggccaaggt
      361 cgccgccgcc cgcgccatat gcctgtccac cggcgtcgca gccgatctgg cgaagcattc
      421 ggcgacggag gaagagcgcc gccgctggaa gggccgcgag gacttgttca cccccatcgc
      481 caaggcatgg tcgaccgacg tcggcgtcga ggtcgcctcg ctgggggtcc agatccacgg
      541 tggaatgggc ttcatcgagg aaaccggcgc ggcccagcac tatcgcgacg cccgcatcgc
      601 cccgatctac gagggcacca acggcatcca ggccatggat ctggtcggcc gcaagctgtc
      661 gatggacggc ggccagtcgg cgcgcgacct catcgcggac atgaagctga ccctcgcaca
      721 gctgccggcc ctgtattccg gcaagccggt cgagcggttc gcggcagcga tctcggcggt
      781 cgaggacgcc accctgtggc tgctggaccg caaggccgcc gcggacggag gcgccgacgt
      841 gctggccgcg gcggacgcct atctgaagct gctgggcgac gtcgcgggcg gctggatgct
      901 ggccgacggc gcgctctacg cccgcgacca gctggacggc ggtcagggcg accctgcgtg
      961 gttggaaggc aagatcaccc tgtacgaggt ctacgccgcc aacgttctgg gccacgcctc
     1021 aagccgcctc gccgccgtgg gtcagggcgg ggcgctgctg gagcggatga gcgtggacgt
     1081 cctggcggga tagccgcccg tcagatgtgg atcacgcgtc cataggcgtc cagcgccgcc
     1141 tcgtgcatgg cctcggacat ggtcgggtgc gggtagacga tgccgtggat gtcctcctcg
     1201 gtcgcttcca tggcgatggc ggtgacatag ccctggatca tctcggtgac ctccgcgccg
     1261 atcatgtggg cgccgatcag ggcgccggtc ttcgcgtcga agatcacctt gacgaagccc
     1321 tcggtctcgc cggcggccac ggccttgccg ttcacccgga aggggaagcg ccccaccttg
     1381 acctcgcgct tcgcctcgcg cgcggcctgt tcggtgatcc cgaccgaggc gacctgcggc
     1441 tgggcgtagg tgcagccggc gatgggcgag ttgacgttcg gcgtcttgta gccggcgata
     1501 tattctgcgg cgtgaatgcc ttcgtgcgag gccttgtgcg ccagccacgg cgcgccggcg
     1561 cagtcgccga tggcgtagag acccttcacg ttcgtctggc agtggccgtc gatggtgatg
     1621 tggccgcggt ccatgttgac ccccagcgcc tccagaccga tgccgtcggt gttggcggtg
     1681 atgccgaccg ccgaaatgca gacctcggcc tcgagggttt ccgccttgcc gccggcctcg
     1741 atggccacct gcacgccctt gccgcccttc gacaccttcg tgaccttgca gccggtgcgg
     1801 aacttcatgc cacgtttctc gaaggccttc tgcgccgcct tggacacttc ctcgtcctcg
     1861 accggcatga tccggtcgac ggcctcgacc acggtcacct cggcgccgag cgcccgatag
     1921 aagctgccga actcgacccc gatgg
//
LOCUS       DASVVN010000524         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_203307,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000524 DASVVN010000000
VERSION     DASVVN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203307"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VF686_08515"
     CDS             <1..803
                     /locus_tag="VF686_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8661897.1"
                     /translation="EESLRILLQTAADSGVTRVATRPDGDGERWLGQSWPFPSPFQVS
                     VRTVPVSDGVDRVEARARRSLERLGLPRGDALLVTSASDLAGTEGRALWDKLLALKDR
                     GLYRRIGFCASVEDGPALLARRFQPDIVQLKCNLLDQRAQHTGVLSELASLDVTVHAS
                     SIFADGLLFMNSEALPVEMHRHAPTLSRIRRRLAEARVDPMQATLAYALGLPEVSAVV
                     ASVASAAELRAVVAAAHAPTPALDWASMQLEPATASAAAGPRRISSAA"
     gene            complement(787..2616)
                     /locus_tag="VF686_08520"
     CDS             complement(787..2616)
                     /locus_tag="VF686_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8661898.1"
                     /translation="MTRSILYATAAAVLFPLSAHAQESDDVLPEVVVTATRLPAIVAD
                     TPGARVIDRQAIEQRGAVFAADILHDIPGLSVVRSGAFGGVAQVRMRGASPGKTLVLV
                     DGIPVNDPAEVNGAFDFSGTELSDIERIEVLSGPQSSLWGSDAIGGVIAFTTRELDGF
                     GADLEAGSYETLRGRLAAGVATERYGFGAWVSHFDTDGISAADEADGNTEADGFTTTT
                     IGARGRYAFTPAVSVDGSARWTDSDVDLDGYPAPAFVLADTLDSQSSEQWSGFGRLRL
                     NALGLAHQFSISASDIARETVSAFPSAFEADRQVFRWQADGSGGGVDFVVGAEREDTE
                     GSLSTGLTEELGTTSAFATARVEPTARLSLTGALRFDDTDDFGSKTTGRVSGAFETGG
                     GFILSAAYGTGFKAPSISQAVCDFCFSAQPFPVLRPETAEGYEGAIGWGSADGRIEGR
                     LTFYRLNVEDQITYVFDPVTFDSFYVNVAETATDGVEFEGRGRLGGGFDLTVAYAWTD
                     ARDESNGARLLRVPEQAGSATLGWTGQRLSGALTVRAEGDQDDAGGVREAFITANLNG
                     SYALTDKVTLTARIENLADERYQQVFGYGEPGRSGYVGVRLRY"
     regulatory      complement(2781..2996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3048..>3658
                     /locus_tag="VF686_08525"
     CDS             3048..>3658
                     /locus_tag="VF686_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX8661899.1"
                     /translation="MNAPLVHPPKADAVPSSEGTTPVMAQFLAAKASQPDAIVFFRMG
                     DFYELFFKDAEIASAALGITLTKRGKHQGEDIPMAGVPVHALDGYLARLIRQGFKAAI
                     CEQLEAPSEARKRGSKAVVHRDIVRVVTPGTLTEDSLLDARGANRLAAVSVRKGRAAV
                     AVVELSAGSVDAVACSLEDLGATLASFRPSEVLVPDRLFSDEAT"
BASE COUNT          544 a         1407 c         1168 g          539 t
ORIGIN      
        1 gcgaggaatc gctgcgcatc ctgctccaga ccgccgccga ttccggcgtc acccgcgtcg
       61 ccacccgccc cgacggcgac ggcgaacgct ggctgggtca gtcctggccc tttccctcgc
      121 ccttccaggt ctcggttcgc accgtgccgg tcagcgacgg cgtcgaccgc gtcgaggccc
      181 gcgcccgccg ctcgctggag cggctcggcc tgccgcgcgg cgacgccctg ctcgtgacct
      241 cggcttccga cctcgcgggc acggaaggcc gcgccctctg ggacaagctt ctggccctca
      301 aggaccgcgg cctgtaccgc cgcatcggct tctgcgcctc ggtcgaggac ggccccgccc
      361 tgctggcccg ccgcttccag cccgacatcg tccagctgaa atgcaatctg ctcgaccagc
      421 gcgcccagca caccggcgtc ctgtccgaac tggcctcgct ggacgtgacc gtccacgcct
      481 cgtcgatctt cgccgacggc ctgctgttca tgaacagcga agccctgccg gtcgagatgc
      541 accgccacgc cccgaccctg tcccgcatcc gccgccgtct ggccgaggcc cgcgtcgatc
      601 cgatgcaggc gaccctggcc tatgcgctgg gcctgcccga ggtctcggcc gtggtcgcca
      661 gcgtcgcctc ggccgccgaa ttgcgcgccg tggtcgccgc cgcccacgcc ccgacgccgg
      721 cgctcgactg ggcctccatg cagctggagc cggcgacggc ttccgccgcc gccggcccgc
      781 gccgcatcag tagcgcagcc tgaccccgac atagccggac cgtccgggct cgccgtagcc
      841 gaacacctgc tgataccgct cgtccgccag attctcgatc cgtgccgtca gcgtgacctt
      901 gtcggtcagg gcgtacgacc cgttcagatt ggcggtgata aaggcctccc gaaccccgcc
      961 ggcgtcatcc tgatcgccct cggcccggac ggtgagcgca cccgacaacc tttgaccggt
     1021 ccagcccagc gtcgccgacc ccgcctgttc cggcacgcgc agcagccgcg ccccgttgga
     1081 ctcgtcccgc gcgtcggtcc aggcgtaggc caccgtcagg tcgaaccccc cgcccagccg
     1141 gccgcggccc tcgaactcga ccccgtccgt cgccgtctcg gccacgttca catagaagct
     1201 gtcgaaggtg accgggtcga agacataggt gatctggtcc tcgacgttca ggcgatagaa
     1261 ggtcagccgg ccttcgatcc gaccgtccgc cgagccccag ccgatcgccc cctcataccc
     1321 ctcggccgtc tcgggccgca gcaccgggaa cggctgggcc gagaagcaga aatcgcacac
     1381 cgcctggctg atcgacggcg ccttgaaccc cgtcccgtag gccgccgaca ggataaagcc
     1441 gccgcccgtc tcgaatgcgc ccgaaacccg gcccgtcgtc ttcgacccga aatcatccgt
     1501 atcgtcgaat cgcagggcgc cggtcaggct gagccgagcc gtcggctcga cacgcgccgt
     1561 ggcgaaggcc gaggtcgtgc cgagctcctc ggtcaggccg gtcgacaaac tgccctccgt
     1621 gtcctcgcgc tcggccccga cgacgaagtc cacgccgccc cccgacccgt cagcctgcca
     1681 gcggaacacc tgccggtcgg cctcgaacgc cgaaggaaag gccgagacgg tctcccgcgc
     1741 gatgtcggac gccgagatgc tgaactgatg cgccagcccc agcgcgttca gacgcagccg
     1801 cccgaacccg gaccactgct cgctggactg gctatccagg gtgtcggcca gcacgaacgc
     1861 cggcgccgga tagccgtcca gatcgacgtc gctgtcagtc cagcgcgccg agccatccac
     1921 actcacggca ggggtgaagg cgtagcggcc cctggcgccg atggtcgtgg tggtgaagcc
     1981 gtcggcctcc gtattaccgt cggcctcgtc ggcggcggaa atcccgtccg tgtcgaaatg
     2041 cgaaacccag gcgccgaagc cgtagcgttc ggtcgcgaca cctgccgcca gccgtccgcg
     2101 cagggtctca taggacccgg cctccagatc ggccccgaag ccgtccagct cacgcgtcgt
     2161 gaaggcgatc accccgccga tggcgtccga gccccacagc gacgactgcg gacccgacag
     2221 cacctcgatc cgctcgatgt ctgacaactc cgtcccggag aagtcgaacg ccccgttgac
     2281 ctcggccggg tcgttgaccg ggatgccgtc gaccagcacc agagtcttgc cgggcgaggc
     2341 gccgcgcatc cgcacctggg cgactccccc gaacgctcca gaacgcacca ccgacaggcc
     2401 ggggatgtca tgcagaatgt cggcggcgaa caccgccccg cgctgctcga tggcttggcg
     2461 gtcgatcacc cgcgcgcccg gcgtgtcggc gacgatggcc ggaagacgag tggcggtgac
     2521 caccacctcg ggcaagacgt cgtcggattc ctgcgcgtgg gcggacaggg gaaacagcac
     2581 ggcggctgcg gtggcgtaga ggatggatcg cgtcatgcgc gggtctccgt ctgccccgcg
     2641 ctggcgctgc gcggcggcgt gtgaaagcga ccgacccggc cccggatcgg ggcgcggcgc
     2701 ttgggtcggg tcgcttcgac ggaacagctt ccgcgcgagc ggaagggttg gccccacgcc
     2761 tctgtctggt cccggacagc ggacgagcga cgtcggcggc caggtctcct ggcttgcggg
     2821 tcaaaaacgg aggatcgcgc cttcccggac atttcccgaa ggaatgggcc agtgacgccg
     2881 aagccgaagc cccggaatga tcgttcgtct cgccgcctac agttgcgggc acagccgcgg
     2941 tgtctcaccg cgttccctta accgcctgcc cggcttgtcg cagtgcagca cagctttcgc
     3001 aagacgcttg aggcggacgc tgcaatcctt caccttgcgg cgctcttatg aacgcccccc
     3061 tcgtccaccc tccgaaagcc gacgcggtcc cgtccagcga ggggacgacg cccgtcatgg
     3121 cgcaattcct cgcggccaag gccagccagc ccgacgccat cgtgtttttc cgcatgggcg
     3181 acttctacga gctcttcttc aaggacgccg agatcgcctc cgccgctctg ggcatcaccc
     3241 tgaccaagcg cggcaagcat cagggcgagg acattcccat ggccggcgtg ccggtccacg
     3301 ccctcgacgg ctatctggcc cgattgatcc ggcaggggtt caaggccgcg atttgcgaac
     3361 agctggaggc gccgtccgag gcccgcaagc gcggctccaa ggccgtggtc catcgcgaca
     3421 tcgtccgcgt cgtcaccccc ggcaccctga ccgaggacag cctgctggat gcgcgcggcg
     3481 ccaaccgcct ggcggccgtt tcggtgcgca agggccgcgc cgccgtggcc gtggtcgagc
     3541 tgtcggccgg ttcggtcgac gccgtcgcct gctcgctgga agacctcggc gccaccctcg
     3601 cctccttccg cccgtccgaa gtgctggtgc ccgaccgcct gttctcggac gaggcgac
//
LOCUS       DASVVN010000525         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_125232,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000525 DASVVN010000000
VERSION     DASVVN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125232"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="pstC"
                     /locus_tag="VF686_08530"
     CDS             <1..1054
                     /gene="pstC"
                     /locus_tag="VF686_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8661900.1"
                     /translation="RFATIGGAVAVLLALGAAWFALRRTSPQIRARTGVERWLMAVLF
                     AASLIAILTTLGILLSLMFESLRFFQMVAPTDFLFGTQWSPQTAMRADQAGSSGAFGS
                     VPLFWGTIFIGAIIAMIVAIPLGLMSAIYLTQYAHPRVRSWMKPILEILAGVPTVVYG
                     FFAALTVGPAFRVFFNEIGAFLVGGPLSGLGQYLMLVQNQMALVAGMVMGIMLIPFVS
                     SLSDDIINAVPQSLRDGSYAMGATKSETVSRVLLPAALPGVAGAMLLAMSRAIGETMI
                     VTMAAGLAARLTLNPLDTVTTVTVQIVTLLTGDQEFNSPKTLSAFGLGLTLFLVTLVL
                     NIVAQRIVQTYRQQYD"
     gene            1047..>2175
                     /locus_tag="VF686_08535"
     CDS             1047..>2175
                     /locus_tag="VF686_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstA family ABC transporter permease"
                     /protein_id="HEX8661901.1"
                     /translation="MTDATVTPEMRADRLRAGLKRRYARESRFKLYGLLAIGVAVGFL
                     LLLLGRIIEQGHTAFYAHTVTVPVYMDPARVDRGYPQGTNFELLVAEQQLARFGVADD
                     AQGSKATAMRSLFSSELKFAAAARIQSNPNLIGQTVPIAAPVSDDADLFFKGEISRDT
                     PADQRRLTDEQIGWLDRMKASGHVAAHFNSGLFTKGDSTEPELAGVLGAVVGSALMLL
                     VTAFIAIPLGVGAALYLEEFAPRGRITDLIEVNINNLAAVPSIIYGLLGLALFINWMH
                     LPRASPLVGGLVLALMALPTIIIATRSSLKAVPPSIREAALAMGASKTQTVFHHTLPL
                     AMPGVMTGAIISMAHALGETAPLLLIGMVSFIPGIPHSVGEP"
BASE COUNT          316 a          797 c          665 g          397 t
ORIGIN      
        1 gcgctttgcg accattggcg gagcggtcgc ggtcctgctc gcgctcggcg ctgcatggtt
       61 cgcgctgcgc cggacctcgc cacaaatccg tgcccgcaca ggcgtcgaac gatggctgat
      121 ggcagtgctc tttgcagcct cgctgatcgc catattgacg acgctcggaa tcctcctttc
      181 gctgatgttc gagagcctgc gcttcttcca gatggtggcg ccgaccgact tcctgttcgg
      241 cacccagtgg agcccgcaga ccgcaatgcg cgccgaccag gcgggatcgt cgggcgcctt
      301 cggctcggtg cccctgttct gggggacgat cttcatcggc gcgatcatcg ccatgatcgt
      361 tgccatcccg ctcggcttga tgagcgccat ctatttgact caatacgccc acccgcgagt
      421 gcgctcgtgg atgaagccga tcctcgagat tctcgccggc gtgccgaccg tcgtctacgg
      481 cttcttcgcc gccctgaccg tggggccggc cttccgcgtc ttcttcaacg agatcggcgc
      541 ctttctggtc ggcggtccgc tgagcgggct ggggcagtat ctgatgctgg tccagaacca
      601 gatggccctc gtcgccggca tggtcatggg catcatgctg atcccgtttg tcagctccct
      661 gtccgacgac atcatcaacg ccgtgccgca atcgctgcgc gacggctcct acgcgatggg
      721 cgcgacgaaa tccgagacgg tcagtcgggt gctgcttccg gccgccctgc cgggtgtcgc
      781 cggggccatg ctgctggcca tgtcgcgggc catcggcgag accatgatcg tgaccatggc
      841 cgccggcctc gccgcccgcc tgaccctgaa cccgctggac accgtcacca ccgtgaccgt
      901 ccagatcgtc accctgctga cgggcgatca ggagttcaac agccccaaga ccctgtccgc
      961 cttcggcctc ggcctgaccc tgttcctggt gaccctggtg ctgaacatcg tcgcccagcg
     1021 catcgtccag acctatcggc agcaatatga ctgacgcgac cgtcacgccc gagatgcgcg
     1081 ccgaccgcct gcgcgccggc ctgaagcgcc gctacgcccg cgagagccgg ttcaagctgt
     1141 acggcctgct ggccatcggc gtggccgtgg gcttcctgct gctgctgctg ggccgcatca
     1201 tcgaacaggg ccacacggcc ttctacgctc acacggtcac cgtgccggtc tatatggacc
     1261 ctgcccgcgt cgaccggggc tatccgcagg ggaccaattt cgaactgctg gtcgccgagc
     1321 agcagctggc ccgtttcggc gtcgccgacg acgcccaggg ctcgaaggcg accgcgatgc
     1381 gcagcctgtt ctcctcggaa ctgaagttcg ccgccgccgc ccgcatccag agcaatccca
     1441 acctgatcgg ccagacggtg ccgatcgccg cccccgtgtc cgacgacgcc gacctgttct
     1501 tcaagggcga gatttcgcgc gacaccccgg ccgaccagcg ccgcctgacc gacgaacaga
     1561 tcggctggct ggaccggatg aaggcctcgg gccatgtcgc ggcccacttc aacagcggcc
     1621 tgttcaccaa gggcgactcc accgagccgg aactggccgg ggtgctgggc gccgtggtcg
     1681 ggtcggccct gatgctgctg gtcaccgcct tcatcgccat tccgctgggc gtcggcgcgg
     1741 ccctgtatct ggaagagttc gccccgcgcg gccggatcac agacctgatc gaggtcaata
     1801 tcaacaacct cgccgccgtg ccgtcgatca tctacggcct gctgggtctg gccctgttca
     1861 tcaactggat gcacctgccg cgcgccagtc cgctggtcgg cggtctggtc ctggccctga
     1921 tggcactgcc gaccatcatc atcgccaccc gctcgtcgct gaaggccgtg ccgccctcga
     1981 tccgcgaggc cgccctcgcc atgggcgcgt cgaagaccca gaccgtcttc catcacaccc
     2041 tgccgctggc catgccgggt gtgatgaccg gcgccatcat ctccatggcc cacgccctgg
     2101 gcgaaaccgc ccccctgctg ctgatcggca tggtcagctt cattccgggc attccgcaca
     2161 gcgtgggcga gccca
//
LOCUS       DASVVN010000526         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_45950, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000526 DASVVN010000000
VERSION     DASVVN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45950"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1289)
                     /locus_tag="VF686_08540"
     CDS             complement(174..1289)
                     /locus_tag="VF686_08540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8661902.1"
                     /translation="MTALQPRRVMMTTDAVGGVWTYALDLSAGLRARGMEVTLAVLGP
                     SPQPGQRRAAERIEGLGLVDTGLPLDWTARNAGAVAAAAQEVARLATVHGSDLIHLNT
                     PALAAEGRFAAPTIGACHSCLASWWSTVRGEEPMPESFRWRTDLLARGYAACDRLIAP
                     SYAFMQETAALYGVLPQAVRNGRSPVTGKRLPDRNPIVLTSGRLWDEGKNLRTLDRAA
                     GRMRGQIHAAGPVKGPDGQTVAFDHILCLGQLDAAVLAKHLERSAVFASLSLYEPFGL
                     GVLEAAQAGCALVLSDIPTFRELWQGAAVFVPADDDAAAAEALDGLLDDPERAAALGR
                     CAAERSARFTVDAMVEATLAVYGEALAARAPFPEVAA"
     gene            complement(1286..2329)
                     /locus_tag="VF686_08545"
     CDS             complement(1286..2329)
                     /locus_tag="VF686_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661903.1"
                     /translation="MSRILITGGAGFIGSNLADRLAGAGHEVVLMDTLARPGVEQNLK
                     WLLLRHRSRVRNLQTDVRDGAGVRAAVADVNAVFHLAAQVAVTTSLSDPREEFEINLG
                     GALNVLEAARERGVPVIFASTNKVYGDLADVDLELVDDAWTPVDPALRAAGVNERRPL
                     DFHTPYGCSKGAADQYVLDYARSFGVPACVMRMSCIYGPRQMGTEDQGWVAHFLIRAL
                     NGEPITLFGDGRQVRDILHVNDAIAAYVAALERIDSVSGRAFNLGGGPANAVSLRQLI
                     THIESLTGRRADVRFEDWRAGDQRYFVADASAARALLDLPEPTGWREGVADLARWLRS
                     ESTLVSAPQTAAA"
     gene            complement(2326..3438)
                     /locus_tag="VF686_08550"
     CDS             complement(2326..3438)
                     /locus_tag="VF686_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8661904.1"
                     /translation="MGDTILITGGAGFVGRHVARALLEKGHRVRALDGLIEQVHPTCT
                     RPDELDPEVELMVGDIRDETAVLAALSGVDKVIHLAAEVGVGQSMYAIDRYTSVNDYG
                     TAVLFQQLIDQPVERVVVASSMSIYGEGLYRTADGELLQDVVRGPRNPDGSWDPLDAQ
                     GRPLIPVPTPETKQPALKSVYAINKFVQERLTLTLTAQYGMESCALRLWNIYGPGQAL
                     SNPYTGVLAIFASRIANGQPPMVFEDGQQRRDFVHVSDVARAFLLALEKPEADGQVFN
                     IGSGEDRTVEEVARLQAASMGRADLAPQITQQARAGDIRHNIPDLSKARDLLGYEARA
                     DFAEGLEELADWVARQESEDRVVEARRELEMRGLVA"
BASE COUNT          555 a         1234 c         1207 g          578 t
ORIGIN      
        1 cagccccgcc tcgccgtggt ctgcgatcag gttggcgcgg ctccaggcgt cgcgcggttc
       61 atggaccgcc gcctcgtatc cgcgggcgat cagatcgcgc aggacgccgc gcaggaaatg
      121 cgcattgccg tggttccagc acgactcaag ggagtgggtg aaatagaccg ccttcacgcc
      181 gccacctccg ggaagggcgc tcgggcggcg agcgcctcgc cgtagacggc gagcgtcgcc
      241 tcgaccatgg cgtcgacggt gaagcgggcg cttcgttccg ccgcgcagcg gcccagcgcc
      301 gcggcgcgtt cgggatcgtc gagcaagccg tccagcgcct cggccgcggc agcatcgtcg
      361 tcggcgggaa cgaacaccgc cgcgccctgc cacagctccc gaaaggtcgg gatgtcggac
      421 aggaccagcg cgcacccggc ctgcgccgcc tccagcacgc ccagaccgaa cggctcgtag
      481 agcgacaggg aggcgaaaac ggccgaccgt tccagatgct tcgccagcac cgccgcgtcg
      541 agttgaccga ggcagaggat gtgatcgaag gcgaccgtct gtccgtccgg tcccttcacc
      601 ggtccggcgg cgtgaatctg cccccgcatc cgccccgccg cccggtccag cgtgcgcagg
      661 ttcttgcctt cgtcccagag acggcccgaa gtcagaacga tcggattgcg gtccgggagg
      721 cgctttcctg tgacgggcga gcgtccgttg cggaccgcct gcggcagcac gccgtagagg
      781 gccgctgttt cctgcatgaa ggcgtagctg ggcgcgatca ggcggtcgca ggcggcatag
      841 ccgcgggcca gcagatcggt acgccagcgg aacgattccg gcatcggctc ctcgccccgg
      901 accgtactcc accagctggc cagacaggag tggcaggcgc cgatggtcgg cgcggcgaac
      961 cgaccctcgg cggccagggc cggcgtgttc agatggatca gatcggagcc gtggacggtc
     1021 gcgagtctcg ccacttcctg agccgccgcg gcgacggccc cggcgtttct ggccgtccag
     1081 tcgaggggta gtccggtgtc gacgagcccc agtccctcga tcctttccgc agcccggcgt
     1141 tgtccgggct gcggcgacgg accgagcacc gccagcgtca cctccatacc gcgcgcgcga
     1201 agaccggctg aaagatcgag cgcataggtc cagacgccgc cgaccgcgtc cgtggtcatc
     1261 atcacgcggc gcggctggag ggccgtcatg ctgcggccgt ctgcggagcg ctgacgaggg
     1321 tgctttcgga ccgtagccag cgggcgagat cggccacgcc ttcgcgccac cccgtcggct
     1381 ccggcaggtc cagcagcgcc cgggcggccg aggcatcggc gacgaagtag cgctgatcgc
     1441 cggcgcgcca gtcttcgaag cgcacatccg cgcgtcgacc cgtcagactc tcgatgtggg
     1501 tgatcagctg acgcaggctc accgcattgg ccggtccgcc tccaaggttg aaggcgcggc
     1561 cactgacgct gtcgatccgc tcgagagcag cgacataggc cgcgattgca tcgttgacgt
     1621 gcaggatgtc gcgaacctgc cggccgtcgc caaacagggt gatgggctcg ccgttcagcg
     1681 cccggatcag gaaatgggcg acccagccct ggtcctccgt tcccatctga cgggggccgt
     1741 agatgcagct catccgcatg acgcaggcgg gcacgccgaa gctgcgtgcg tagtcgagca
     1801 catactggtc agccgcgccc ttggaacagc cgtagggcgt gtggaaatcc agcggtcgtc
     1861 tttcgtttac gcctgcagcg cgcaaggcgg gatcgaccgg cgtccatgcg tcgtcgacca
     1921 gctcgagatc cacgtcagcg aggtcgccgt agaccttgtt ggtgctggcg aagatcaccg
     1981 gcacgccccg ttcccgggcc gcctccagaa cgttcagggc gccgcccaga ttaatctcaa
     2041 actcttcccg ggggtcggac aggctggtgg tgacggcgac ctgcgccgcg agatggaaga
     2101 ccgcgttgac gtcagcgacg gctgcacgca cgcccgcgcc gtcgcgcaca tcggtctgca
     2161 ggttgcgcac ccgtgagcgg tggcgcagca aaagccattt caggttctgc tcgacgcccg
     2221 gccgcgccag ggtgtccatc agaacaacct cgtggccggc gcccgcgagc cgatcggcga
     2281 ggttgctgcc gataaatccg gcgccgccgg tgatcaggat gcggctcatg cgaccagacc
     2341 ccgcatctcg agttcgcggc gggcctcgac cacccggtcc tcggactcct gccgcgcgac
     2401 ccagtcggcc agttcttcca gcccctcggc gaagtctgcg cgcgcctcgt aaccgagcag
     2461 gtcgcgggcc ttcgacaggt cgggaatgtt gtgccggatg tcgcccgcgc gggcctgctg
     2521 ggtgatctgc ggcgcgagat cggcgcgtcc catggaggcc gcctgcagcc gcgccacctc
     2581 ttccaccgtc cggtcctcgc cggagccgat gttgaagacc tgcccgtccg cctcgggctt
     2641 ctccagcgcc agcaggaagg cgcgggcgac gtcggagacg tgcacgaagt cgcgccgttg
     2701 ctgcccgtcc tcgaacacca tgggtggctg gccgttggcg atccgcgacg cgaatatggc
     2761 cagcacgccg gtgtagggat tggagagcgc ctgccccggc ccgtagatgt tccacagccg
     2821 cagggcgcaa ctctccatgc cgtactgggc ggtcagggtg agggtcagcc gctcctggac
     2881 gaatttgttg atcgcgtaga cgcttttcag cgccggctgt ttcgtctccg gcgtcgggac
     2941 cggaatcagc ggccgcccct gcgcatccag cgggtcccag ctgccgtcgg ggttcctcgg
     3001 gccgcgcacc acgtcctgca gcagttcgcc atcggcggtg cggtacaggc cctcgccgta
     3061 gatgctcatc gaggacgcca cgaccacccg ctcgaccggc tggtcgatca gctgctggaa
     3121 cagcaccgcg gtgccgtagt cgttgaccga ggtgtagcgg tcgatggcgt acatgctctg
     3181 tccgacgccg acctcggcgg ccagatggat gaccttgtcg acgccggaga gggcggccag
     3241 caccgccgtc tcgtcgcgga tgtcgccgac catcagttcg acctcgggat ccagttcgtc
     3301 cggccgggtg caggtcgggt gaacctgctc gatcagtcca tcgagcgcgc ggaccctgtg
     3361 ccctttttcc agcagcgccc gcgccacatg ccgacctacg aatcccgcgc cgccggtgat
     3421 caggatggtg tcgcccatga ggtcctctcc ggttcaggtt caggggagca acatcttctt
     3481 ctacaggtta gttccgcgcc tgcttatgta gtctattgga catatgacga tatctgcttg
     3541 tccagcattg gaaaatttac tcaatgaaag tagc
//
LOCUS       DASVVN010000527         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_98302, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000527 DASVVN010000000
VERSION     DASVVN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98302"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF686_08555"
     CDS             <1..265
                     /locus_tag="VF686_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661905.1"
                     /translation="PESEAEAVTAPVEPNEAEPEAAPAVLDVTPVEAAEVPAPARPER
                     KQAPVVEHVAPPQPEVDVAALIAEDPNQIIAPPEKPKRGWWRR"
     gene            332..1747
                     /locus_tag="VF686_08560"
     CDS             332..1747
                     /locus_tag="VF686_08560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8661906.1"
                     /translation="MNWLSRNAAVRLSGAVAAVGLLLATASPAAAQSAIRDTEIEGII
                     HEWTDPVLVAMGLEPSEIEILLINENDLNAFATRGRIMGVNTGLILKTRNANELLGVL
                     AHEAGHIKNRHTLRDGAQGAGMQPMLMTMALGALAIAAGAPDAGAVLLGNSQYFGTLS
                     ALRYMTHQEGEADNTGARALQNAGESGRGLVSFFENFRSQEVFSDARRYPYFRSHPLS
                     SDRIENLRRFVAEQSNYNHQDSPERIAEHALILAKIHAFMDAPNQTLRDYPESDTSFP
                     ARYARAIAWYRDGQTDRALAAVDAFLTEQPGNPYFWELKGQILFEEGRPAEAIGAHQR
                     SVELKPDAPLLRINLAHALIETRDPAKLDEAVNQLKRATALEHDNTMGWRLLSQAYAS
                     QNKEGEARLASAEMYFAMGAERQATQFALRARDMLTPGTAEWTRAMDIVFASGATQDD
                     IDDVDRRNSRRDRGGVIPAAG"
     gene            1768..2550
                     /locus_tag="VF686_08565"
     CDS             1768..2550
                     /locus_tag="VF686_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8661907.1"
                     /translation="MTDAPEPTPARDRGFFDRPRAAMASMAVSVVALGLAAAPYAQGT
                     VDFDVRVRTYLLKNPEILQEVSAALQAKEDGARTDAINQRAAASPSLLAVSAQDPAFG
                     PADAKVTVIEFFDFRCPGCKATAPDVLRLMQAHPDVRFVFKDWPILDRGNDDVSHYAA
                     RAAQAAHRQGKYLPVFRALMAEPALSKAAVDRILAANGIAAPAARTAMSSPETARHLA
                     DVQTSAAGLGLLGTPTFFVNGRANPSIQPADIDRAIRAAKAG"
BASE COUNT          451 a          982 c          860 g          372 t
ORIGIN      
        1 gcccgaatcc gaagccgaag ctgttacggc gccggtcgag cccaatgagg cggaacccga
       61 agccgcgcct gcggtgctgg acgtcacgcc cgtcgaggcg gcggaggttc ccgctcccgc
      121 caggccggag cggaaacagg ccccggtcgt cgagcatgtc gccccgccgc agccggaagt
      181 cgacgtcgcc gccctgatcg cggaggatcc gaaccagatc atcgctccac ccgagaagcc
      241 gaagcgcgga tggtggcgcc gctgaaacgc cgtgattagg ttgtgaccat cggccgggcg
      301 ggggcgtaga cttgggtatg aggagttcgc agtgaactgg ctgtcccgaa acgccgccgt
      361 ccggctgtcc ggcgcggtcg ccgccgtcgg cctgttgctg gccacggctt cgcccgccgc
      421 ggcgcagagc gcgattcgcg acaccgagat cgaaggcatc atccatgagt ggacggaccc
      481 cgtgctggtc gccatggggc tggagccgtc cgagatcgaa atccttctga tcaacgagaa
      541 cgacctcaac gccttcgcga cgcggggtcg gatcatgggc gtcaacaccg gcctgatcct
      601 gaagacccgc aacgccaacg aacttctggg cgtcctcgcc cacgaggcgg ggcacatcaa
      661 gaaccgccac accctgcgcg acggggccca gggcgccggc atgcagccca tgctaatgac
      721 catggcgctg ggcgcgctgg cgatcgcggc gggcgcgccg gacgccgggg cggtcctgct
      781 cggaaacagc cagtatttcg gcaccctcag cgccctgcgc tacatgacgc accaggaggg
      841 cgaggccgac aacaccggcg cgcgcgcgct gcagaacgcc ggcgaatccg gccgcggact
      901 ggtctcgttc ttcgagaatt tccgctcgca ggaagtcttc tcggacgccc gccgctatcc
      961 ctatttccgc agccaccccc tgtcttccga ccggatcgag aacctgcgcc ggttcgtcgc
     1021 cgaacagtcg aattacaacc atcaggacag ccccgaacgg atcgccgaac acgcgctgat
     1081 cctggccaag attcacgcct tcatggacgc gccgaaccag acgctgcgcg actacccgga
     1141 gagcgacacg tccttccccg cccggtacgc ccgcgccatc gcatggtatc gcgacggtca
     1201 gaccgacagg gcgctggccg ccgtcgacgc cttcctgacg gagcagcccg ggaaccccta
     1261 tttctgggag ctgaaaggtc agatcctgtt cgaggaaggc cggccggccg aagccatcgg
     1321 cgcgcaccag cgctcggtcg agctgaagcc tgacgccccc ctgctgcgta tcaacctggc
     1381 ccatgccctg atcgaaactc gcgaccccgc caagctggac gaggccgtga accagttgaa
     1441 gcgcgctacg gcgctggagc acgacaacac aatgggctgg cgtctgttga gccaggccta
     1501 cgccagccag aacaaggagg gcgaagcccg cctcgcctcc gccgagatgt atttcgccat
     1561 gggcgctgaa cggcaggcga cccagttcgc cctgcgggcc cgggacatgc tgacccccgg
     1621 cacggccgaa tggacccgcg ccatggacat cgtcttcgcc tccggcgcga cccaggatga
     1681 catcgacgat gtcgaccgcc gcaacagccg ccgcgaccgc ggcggcgtca tccccgccgc
     1741 cggctgaccc ctttttccga gaccgatatg accgacgcgc ccgaacccac gcccgcgcga
     1801 gatcgcggct tcttcgatcg cccgcgcgcg gcgatggcct ccatggccgt gtccgtcgtc
     1861 gccctgggac tggctgcggc tccctacgcc cagggtaccg tcgacttcga cgtcagggtg
     1921 cgcacctatc tgctgaagaa cccggaaatt ttgcaggagg tcagcgcggc gctgcaggcg
     1981 aaggaagacg gcgcccgcac cgatgcgatc aaccagcgcg ccgcggccag cccatcactg
     2041 ctggcggtca gcgcgcagga ccccgccttt ggaccggccg acgccaaggt cacggtcatc
     2101 gagttcttcg atttccgctg cccgggctgc aaggcgacgg cgccggacgt ccttcgcctg
     2161 atgcaggccc accccgatgt ccgtttcgtc ttcaaggact ggcccatcct cgatcgcggc
     2221 aatgacgacg tctcgcacta cgccgcgcgc gcggcccagg ccgcgcaccg tcagggcaag
     2281 tatctgcccg tgttccgggc cttgatggcc gaacccgcgc tgagcaaggc ggccgtcgac
     2341 cgcatcctcg cggctaacgg catagcggcc cctgcggcgc gaacggcgat gtcgagcccg
     2401 gagacggcgc ggcatctggc ggatgtccag acctcggccg ccggtctggg cctgctcggc
     2461 acgccgacct tcttcgtcaa cggccgcgcc aaccccagca tccagcccgc cgatatcgac
     2521 cgcgccatcc gggcggcgaa ggcaggctga gcccgccccg ccggctctag gcgatcgccc
     2581 cgacgcagcc ctcgtccccg ccgaccggca ggacggcgtc ggtcagcgac agggtccagc
     2641 cgtggtgggt cgccagaccg cgccg
//
LOCUS       DASVVN010000528         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_494089,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000528 DASVVN010000000
VERSION     DASVVN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494089"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..899
                     /locus_tag="VF686_08570"
     CDS             117..899
                     /locus_tag="VF686_08570"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX8661908.1"
                     /translation="MSDLPLPDLRVAFVASDRPEAQSARERLISRYGSAEPDQAQVIV
                     ALGGDGFMLETLHTVMDRRIPVFGMNRGSIGFLMNDYEEEGLLERLAGAGRAVIHPLQ
                     MDAWTEAGQVHSGLAINEVSLLRQTRQSAKLRISVDDRVRLEELSCDGCLLSTAAGST
                     AYNLSAHGPIIPLDSRVLALTPISAFRPRRWRGALLPHQAKVCFEVLEADKRPVSATA
                     DNFEVRRVARVEVRERRDIALTMLFDAGRSFEERVLAEQFAS"
     gene            965..1441
                     /locus_tag="VF686_08575"
     CDS             965..1441
                     /locus_tag="VF686_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8661909.1"
                     /translation="MTNPAQVIRPPNTLRMKVGGAFGGIDAGAIAKAEAALKAMSSQF
                     GQWLQDEIVKLDKAQADIRAQGYNAQTAEALYFRAHDLKGLGTTYQYPLVTRLAGSLC
                     KMMDDPAKRMAAPVVLLDAHIDAIKAVVRDQIQTDDHPVGKILAETLEGRVAQHLG"
BASE COUNT          263 a          579 c          544 g          285 t
ORIGIN      
        1 cccgccgctg tcggccacct cggcggcgcg cgccttccgc gcgttgatcg ctgacgctca
       61 tagcgaatta aaagccgccg tttgacttcg cccgccggct cggcttgcgt gggcgggtga
      121 gcgatctccc cctaccagac ctgcgcgtgg ccttcgtggc ctcggatcgc cccgaggcgc
      181 agtccgcccg cgagcggctg atctcacgct atggctcggc ggagccggat caggcccagg
      241 tgatcgtcgc ccttggcggc gacggcttca tgctggagac cctgcatacg gtcatggacc
      301 ggcgcatccc ggtgttcggc atgaaccggg gctcgatcgg cttcctgatg aacgactatg
      361 aggaagaggg attgctggaa cgactggccg gcgccggccg ggccgtcatc catcccctgc
      421 agatggacgc ctggaccgag gccggtcagg tccacagcgg gctggccatc aacgaggtgt
      481 ccctgctgcg ccagacccgc cagagcgcca agctgcgcat cagtgtcgat gaccgcgtga
      541 ggctggagga gttgtcctgc gatggctgcc tgctgtccac ggcagcggga tcgacggcct
      601 ataacctgtc ggcgcacggg ccgatcattc cgctggattc ccgggtcctg gccctgaccc
      661 cgatcagcgc cttccgtcca cgccgttggc ggggcgccct gctgccgcat caggcaaagg
      721 tctgtttcga ggtgctggaa gcggacaaac ggcccgtcag cgccacggcc gacaatttcg
      781 aggtccgccg ggtggcgcgc gtcgaggtcc gggaacgccg cgacatcgcc ctgaccatgc
      841 tgttcgacgc cgggcgctcg ttcgaggaac gggttctggc cgaacaattc gcctcctgag
      901 gccatgcacg tttttttagg gacacgggcc cagactgcct ccggtctgtc ttcaggagct
      961 cgccttgacc aatcccgcgc aggtcatccg tccgcccaac acccttcgca tgaaggtcgg
     1021 cggagctttc ggcgggatcg acgccggcgc catcgccaag gcggaagccg ccctgaaggc
     1081 catgtcttcc caattcggcc agtggctgca ggacgagatc gtcaagctgg acaaggccca
     1141 ggccgacatc cgcgcccaag gctataacgc ccagaccgcc gaggccctgt atttccgcgc
     1201 ccacgacctg aagggactgg gtacgaccta ccagtatccg ctggtgaccc gcctggccgg
     1261 ttcgctgtgc aagatgatgg acgacccggc caagcgcatg gccgcgcccg tcgtccttct
     1321 cgacgcccat atcgacgcca tcaaggccgt ggtccgcgat cagatccaga ccgacgacca
     1381 cccggtgggc aagattctgg ccgaaactct ggagggtcgc gtcgcccagc atctgggctg
     1441 acgggtctcg ccaaatctac tcaagccaac gcccgcctga ccggcgggcg ttttgctgtc
     1501 aggcggcggc gctgagccgg gccttggccg gcagcgggtc gtggtccagc cgttccatca
     1561 gataggccag atcctcgcgc ggcgcgttcg ggcgctgcgt caggaacctc tcgagcatcc
     1621 ggtggcggaa ctcgtctccg gacgtgtcca cgccctcggc ctgaagctga c
//
LOCUS       DASVVN010000529         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_20533, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000529 DASVVN010000000
VERSION     DASVVN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20533"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF686_08580"
     CDS             complement(<1..677)
                     /locus_tag="VF686_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8661910.1"
                     /translation="MRTLLLTAGVAALLIAAGPAMAEEPGAAGPEDPAQLPPVSVLAT
                     RTGTPIDEAPATVSVFTADDIETMLATDIKDLIRFEPGVSVVSQPARFGAALGMAGRA
                     GNEGFTIRGIGGDRVLMIVDGVRSPDGFVFGAQSVGRGGYADLDLMKSVEILRGPASA
                     LYGSDGVAGAVLFTTKDPVDLIRANESFGARARVGYNSADEGLTKSAVVAGRSGAFSG
                     LLAWTGR"
     gene            complement(782..1219)
                     /locus_tag="VF686_08585"
     CDS             complement(782..1219)
                     /locus_tag="VF686_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661911.1"
                     /translation="MVDRSDIGAVVDAMYACISGPAGPRDWATQREIFHPDSRQARTG
                     LDPDGRPTMIVMGREEYEANVTPFFMAHGFFEIEISRRIDVFGNMAHVWSVYEARTAL
                     TDAEPERRGVNSIQLARGPDGLWSILHMIWDNERPGLTVPAPT"
     gene            complement(1219..>1573)
                     /locus_tag="VF686_08590"
     CDS             complement(1219..>1573)
                     /locus_tag="VF686_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX8661912.1"
                     /translation="MFVQQGRKVFKDVVPMVSDMIVAHAADLGIDPTTLKRMWLHQAN
                     INMNQFIGRKVLGREPEPHENVIILDDYANTSSAGSIIAFHLHQEGFAPGDTGLICSF
                     GAGYSAGTVFVRKRA"
BASE COUNT          249 a          480 c          592 g          252 t
ORIGIN      
        1 tcccggccgg tccaggcgag caggccggag aaggcgccgc tgcggccggc gacgacggcg
       61 gatttggtca ggccctcgtc ggccgagttg tagccgaccc gggcgcgggc gccgaagctt
      121 tcgttggcgc ggatcaggtc gacggggtcc ttggtcgtga acaggaccgc gccggcgacg
      181 ccgtccgaac cgtacagggc cgaagcaggg ccccgcagga tctcgaccga cttcatcagg
      241 tccagatcgg catagccgcc gcggccgacg ctctgggcgc cgaagacgaa gccgtcgggc
      301 gagcgcacgc cgtcgacgat catcagcacg cggtcgccgc cgatgccgcg gatggtgaaa
      361 ccctcattgc ccgcccggcc agccatgccc agcgcggcgc cgaagcgggc gggctggctg
      421 acgacgctga cccccggctc gaagcgaatc aggtccttga tgtcggtcgc cagcatcgtc
      481 tcgatgtcgt cggcggtgaa gacgctgacc gtggccgggg cctcgtcgat cggcgtgccc
      541 gtgcgggtgg ccagaacgct gaccggcggg agctgggccg gatcctcggg gcctgccgcg
      601 cccggctctt cggccatggc cggtccggcg gcgatcagaa gggcggcgac gccggcggtg
      661 aggaggagag tgcgcatgtg aacggccccg cttgcagaga ggaggggcaa ttactgcgaa
      721 cgattctcag aatcaaccgc aactacgatg tggttatagc gattgttgga tcagctctcc
      781 gtcaggtcgg cgccggcacg gtgaggccgg ggcgttcgtt gtcccagatc atgtgcagga
      841 ttgaccacag gccgtcgggg ccgcgtgcca gctggatgga gttgacgccg cgccgctcgg
      901 gttcggcgtc ggtgagggcg gtgcgcgcct cgtagacgga ccagacgtgg gccatgttgc
      961 cgaacacgtc gatgcggcgg ctgatctcga tctcgaagaa gccgtgggcc atgaagaagg
     1021 gggtgacgtt ggcctcgtat tcctcgcggc ccatgacgat catggtcggg cggccgtcgg
     1081 ggtcgaggcc ggtgcgggcc tgacgactgt cggggtggaa gatctcgcgc tgggtcgccc
     1141 agtctcgagg gccggcgggg ccggagatac aggcgtacat ggcgtcgacg acggcgccga
     1201 tatcgctgcg gtcgaccatc aggcccgctt tctgacgaag acggtcccgg ccgaatagcc
     1261 cgcgccgaag ctgcagatca ggccggtgtc gccgggcgcg aagccctcct gatgcaggtg
     1321 gaatgcgatg atcgaaccgg cggaagaggt gttggcgtag tcatcgagga tgatgacgtt
     1381 ctcgtgcggt tcgggctcgc ggcccagcac cttgcggccg atgaactggt tcatgttgat
     1441 gttggcctgg tggagccaca tccgcttgag ggtggtcgga tcgatgccga ggtcggcggc
     1501 gtgggcgacg atcatgtccg agaccatggg caccacatcc ttgaagacct tgcggccctg
     1561 ctggacgaac atc
//
LOCUS       DASVVN010000530         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_151507,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000530 DASVVN010000000
VERSION     DASVVN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151507"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF686_08595"
     CDS             complement(<1..639)
                     /locus_tag="VF686_08595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8661913.1"
                     /translation="MAALAAVVFCVAVFAAGRGLASGAMGEVLRVRFGGDAQHTRVVV
                     DLDRTARGQVIQSGDQGQVVVSLAGVAPQRGLNGTGTGLVRGWRVAGAGVASRVELDL
                     ATSAEIERRFLLPPGDGIAHYRYVIDLKATGRAPTRVAARPAPRTPTRSTERPLIYID
                     AGHGGRDPGARGANTNESAVTLAAALALKQELERTGRYRVRLTRESDAYVDLY"
     gene            1130..>2128
                     /locus_tag="VF686_08600"
     CDS             1130..>2128
                     /locus_tag="VF686_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8661914.1"
                     /translation="MSKTMLIDAAHAEETRVAIVEGRQVEEFDFESRAKRQLRGNIYL
                     AKVVRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPVADREAIMAEAHSEDDEHDE
                     HARDHADDESEGGMAEEERLKRRLMRRYKIQDVIKRRQVMLVQVVKDERGGKGAALTT
                     WLSLAGRYCVLMPNTGKGGGISRKITQATDRKRLKAAAAALHVPQGMGLIIRTAGAKR
                     TKAEIKRDYEYLLRLWETIRETTLKSNAPSIIYEEENLVRRAVRDMFDKDFDGIQVEG
                     VEGFKEARDFMRVLMPSQSKKVHLYQGARPLFATNGIEEMLTQIHQPVVPLPSGGYL"
BASE COUNT          372 a          780 c          668 g          308 t
ORIGIN      
        1 gtacaggtcg acataggcgt cgctctcgcg ggtcagccgc acgcgatagc ggccggtgcg
       61 ctccagctcc tgcttcagcg ccagcgccgc cgccagcgtc acggcgctct cgttggtgtt
      121 cgcgccccgg gcgccgggat cgcggccgcc atggccggcg tcgatgtaga tcagcggccg
      181 ttcggtcgac cgggtcggcg tcctcggcgc gggtcgcgcc gccacacgcg tcggcgcccg
      241 gccggtggcc ttcagatcga tcacatagcg gtagtgggcg atgccgtcgc cgggcggcag
      301 caggaagcgg cgctcgatct cggcgctggt ggccaggtcc agttcgacac gcgaggccac
      361 gccggctccc gcgacgcgcc agccgcgcac cagacccgtg cccgtgccgt tcagcccgcg
      421 ctgcggcgcg acgccggcca gcgacaccac cacctggccc tgatcgccgg actggatcac
      481 ctgaccgcgg gcggtccggt ccagatcgac caccacgcgc gtatgctggg cgtcgccgcc
      541 gaagcggacg cgcagcacct cgcccatggc gcccgaggcc aggccgcgcc cggccgcgaa
      601 cacggcgacg cagaaaacga ccgcggccaa agccgccatc gcccactcgc gaacgcccca
      661 acgcgtcagg ttgatacgca acagtccgtg cattcgacgg gaacccacct tcttggttgg
      721 cccatgatga ctcaacgtcg tcgccaacgg gttaaagcgc gcaacgcttt taattcacgc
      781 acgcaatgcc tatgcttggt gtgtccgcga acgatcgcgc cccctgactt caggccgggc
      841 gcgccctcct cgcggcatgt tttcactgat ccgaggactg gccggggtag cacccgccgg
      901 ttcgaagacg ggaccgggac gcccctttcg acggcgcgtc cggccccagg accgtactga
      961 accctcatgg gccccatcgc ctgcgctccc ttccggtttg aaacaaccga cctcgcccgc
     1021 ggccttcgcc gcggcgacgg gactgcgtct gcgcctgccc gcaatcccat tcggcgagcc
     1081 gcctgccccc ttcgcgggga tggccgagcg cgccagaaag acttcttcca tgtcaaagac
     1141 catgttgatc gacgccgccc acgcggagga gacccgcgta gccatcgtcg agggccgtca
     1201 ggtcgaggaa ttcgactttg aatcccgcgc gaagcgccag cttcgcggca atatctatct
     1261 cgccaaggtc gttcgcgtag agcccagcct ccaggctgcg ttcgtggaat acggcggcaa
     1321 ccgccacggc ttcctcgcct tcagcgaaat ccaccccgac tattaccaga tccccgtcgc
     1381 cgaccgcgaa gccatcatgg ccgaggccca ctcggaagat gacgagcacg acgaacacgc
     1441 ccgcgaccac gccgacgacg aatccgaagg cggcatggcc gaggaggaac gcctcaagcg
     1501 ccgcctgatg cgccgctaca agatccagga cgtcatcaag cgccggcagg tcatgctggt
     1561 tcaggtcgtc aaggacgagc gtggcggcaa gggcgcggcc ctgaccacct ggctgtcgct
     1621 ggccggccgc tactgcgtgc tcatgcccaa caccggcaag ggcggcggca tctcgcgcaa
     1681 gatcacccag gccaccgacc gcaaacgcct gaaggccgcc gcggccgcct tgcacgtgcc
     1741 gcagggcatg ggcctgatca tccgcaccgc tggcgccaaa cgcaccaagg ccgagatcaa
     1801 gcgcgactac gaatatctgc tgcgcctgtg ggaaacgatc cgcgaaacca cgctgaagtc
     1861 gaacgccccc tcgatcatct atgaggaaga aaacctcgtc cgccgcgccg tgcgcgacat
     1921 gttcgacaag gatttcgacg gcatccaggt cgagggcgtc gagggcttca aggaggcgcg
     1981 cgacttcatg cgcgtgctga tgccctcgca atcgaagaag gtgcacctgt accagggcgc
     2041 ccgccccctg ttcgcgacca acggcatcga ggagatgctg acccagatcc atcagccggt
     2101 cgtgccgctg ccttcgggcg gctatctg
//
LOCUS       DASVVN010000531         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_231928,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000531 DASVVN010000000
VERSION     DASVVN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231928"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF686_08605"
     CDS             <1..199
                     /locus_tag="VF686_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661915.1"
                     /translation="VTPAKTSNLYYEVVFYSDASHTEWVGDYRQICMNNTTIITPMVN
                     GRTSPYEVSTPIGSCPGLGDW"
     gene            complement(203..1372)
                     /locus_tag="VF686_08610"
     CDS             complement(203..1372)
                     /locus_tag="VF686_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class V aminotransferase"
                     /protein_id="HEX8661916.1"
                     /translation="MSSARKDLFSRALSLDPDRLHFAAHSHHLWPDASFDGQVQAWVE
                     ANRFADRKWDLIFGEVVPKAQTQVARELNLPDPDTVVFAPNTHDLLLRVFSGLERKPV
                     RILSTDGEFHSFRRQGQRWEEAGEALITRVPLEPFDTFDERFVAAAKAGEFDLIVVSQ
                     VFFRTGQVFGGIADLAALADPAGPWVVIDGYHGFMATPTDLGGVADRIFYLAGGYKYA
                     MSGEGVCFLHAPVGYCPRPVVTGWFAEFGDLSGPPGGVQYRSDGGRFWGATFDVSPLY
                     RFNAARAALDEAGLTTADIAAHARTLMQRFQTAVADGETGKLVEADLINPVAGEGSRA
                     RFLAFRHDKASAWKERLIDAGVVTDVRDDVIRFGFGLYQDAADVRRLIEICRRVL"
     gene            complement(1381..>1577)
                     /locus_tag="VF686_08615"
     CDS             complement(1381..>1577)
                     /locus_tag="VF686_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX8661917.1"
                     /translation="LNPGTGENEMADPLAQLPESALQGDDRARLPVDVLPAGWERTPR
                     NSDCPCGSGRKFKHCHGSLI"
BASE COUNT          258 a          555 c          535 g          229 t
ORIGIN      
        1 cgtcaccccg gccaagacgt cgaacctcta ctatgaggtc gtcttctaca gcgacgccag
       61 tcacaccgaa tgggtcggcg actatcggca gatctgcatg aacaacacga ccatcatcac
      121 gccgatggtc aacggccgga cctcgcccta cgaggtcagc accccgatcg gcagctgccc
      181 cggtctcggc gactggtgac gtctacagga cccgccggca gatctcgatc agccggcgga
      241 cgtccgctgc gtcctggtac aggccgaagc cgaagcggat cacgtcgtcg cgcacgtcgg
      301 tgacgacgcc cgcgtcgatc agccgttcct tccaggccga ggccttgtcg tggcggaagg
      361 cgaggaagcg cgcccggctg ccttcgccgg cgaccggatt gatcagatcg gcctcgacca
      421 gcttgccggt ctcgccgtcg gcgaccgcgg tctggaaccg ctgcatcagc gtgcgcgcat
      481 gggcggcgat atccgccgtg gtcagccccg cctcgtccag cgcggcgcgc gcggcattga
      541 accggtacag cggcgacacg tcgaaggtcg cgccccagaa ccggccgccg tcgctgcggt
      601 actggacgcc gcccggcggc ccggacaggt ccccgaactc ggcgaaccag ccggtgacga
      661 ccggccgcgg gcagtagccg acgggcgcgt gcaggaaaca gaccccctcg cccgacatgg
      721 cgtatttgta gccgcccgcc agatagaaga tccggtccgc cacgccgccc agatcggtcg
      781 gcgtcgccat gaagccgtga tagccgtcga tgaccaccca cggtccggcc ggatcggcca
      841 gcgccgccag atcggcgatg ccgccgaaca cctgtcccgt gcggaagaag acctggctga
      901 cgacgatcag atcgaactcg ccggccttgg cggcggccac gaaccgttcg tcgaaggtgt
      961 cgaacggctc cagcggcacg cgggtgatca gggcctcgcc agcctcttcc cagcgctggc
     1021 cctgacgccg gaacgagtgg aactcgccgt cggtcgacag gatgcggacg ggcttgcgct
     1081 ccagacccga gaagacgcgc agcagcaggt cgtgggtgtt gggcgcgaac accaccgtgt
     1141 ccgggtcggg caggttcagc tcgcgcgcca cctgggtctg cgccttcggc acgacctcgc
     1201 cgaagatcag gtcccatttc cggtccgcga accggttggc ctcgacccag gcctgcacct
     1261 gcccgtcgaa gctggcgtcg ggccacaggt ggtggctgtg cgccgcgaaa tgcagccggt
     1321 cggggtcgag cgacagcgcg cgtgagaaca ggtccttgcg agcgctgctc atgtcgagac
     1381 ctagatcaaa ctaccgtggc agtgtttgaa cttgcggccc gagccgcagg ggcagtccga
     1441 gttgcgcggc gtgcgctccc agccggcggg caggacgtcg accggcaggc gggcgcggtc
     1501 gtcgccctgc aaggcgcttt ccggcagctg ggccagcgga tcagccatct cgttctcgcc
     1561 cgtgccgggg ttcaggt
//
LOCUS       DASVVN010000532         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_20618, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000532 DASVVN010000000
VERSION     DASVVN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20618"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VF686_08620"
     CDS             <1..781
                     /locus_tag="VF686_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661918.1"
                     /translation="CTWSTPACAEQAMFENDRRLMTWDLLAGKVTHGHRLRSRLDGFG
                     LSARLDALSTAPCPPALIGLNHYVTSDRFLDDRLHRYPPHVHGGNGRLRYADVEAVRV
                     LHDQPSGWTRSLELLWDRYHIPLAITECHLGCSVDEQRRWLGSAWSAALSARSAGIPV
                     EAVTVWALLGSHDWNSLLTREDGHYETGAFDVSGGDPVPTPLARTIRRLARDGVPGPD
                     LGAGWWETDTRFEYPPCHPCGADASFVPANLASASLIALPK"
     gene            790..>1823
                     /locus_tag="VF686_08625"
     CDS             790..>1823
                     /locus_tag="VF686_08625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8661919.1"
                     /translation="MPQPLSNTPGNRVVELETAHRTEPIVCLSHLRWNFVFQRPQHLM
                     SRFAKSRTVLVFEEPLPVEPGATMGVDLRVCAESGVVVATPRLPDGLDGPSRNAVMRQ
                     LLDEAIAAQNIERPVLWYYTPMMVPIARHIEAAAVVYDCMDELANFRFAPPELTTLER
                     ELFASADVVFTGGYSLYEAKRALHDNIHPFPSSVDTAHFAQARSTPVGRDDRRPRLGF
                     FGVIDERMDLDLISRVAEARPDWDFEMVGPVVKIAAEDLPQRPNLSYPGQKSYDELPA
                     CLARWDVALMPFAINESTRFISPTKTPEYLAAGRPVVSTPIRDVVRHYSELDAVRIAA
                     TPDLFVAGCEA"
BASE COUNT          286 a          650 c          578 g          309 t
ORIGIN      
        1 ctgcacctgg tcgacgccgg cctgcgccga gcaggcgatg ttcgaaaacg accggcggct
       61 catgacatgg gacttgctgg ccggaaaggt gacgcatggc caccggctgc gttcccgtct
      121 cgacgggttc ggtctgtcgg cgcggctgga tgcgctctcg acggccccct gccctcccgc
      181 tctgatcgga ctgaaccact atgtcaccag cgaccggttt cttgacgacc ggctccatcg
      241 ctatcccccg cacgtgcacg gcggcaacgg gcgactgaga tatgcggacg tcgaagccgt
      301 gcgcgtattg cacgaccagc catcgggctg gacgcggtcg ctcgagcttc tctgggaccg
      361 ctatcatatc ccgctggcca tcaccgaatg ccacctcggc tgctcggtcg atgagcaacg
      421 acgctggctg gggtccgcat ggtcggcggc gctttccgcc cggagcgccg gcattccggt
      481 cgaggccgtg accgtatggg ccctgctggg aagccatgac tggaacagcc tgctgacgcg
      541 agaggacggc cactacgaaa caggcgcctt cgacgtttcg ggtggcgatc ccgttccaac
      601 tcccctcgcc cgaaccatcc gccggctggc gcgcgacggg gtgcccggcc ccgacctcgg
      661 cgcgggatgg tgggagaccg acacccgctt cgaatacccg ccctgccacc cctgcggcgc
      721 cgacgcatcc ttcgtcccgg cgaacctcgc gtccgccagc ctcatcgccc tcccaaaata
      781 ggaatacctg tgccccaacc gctttcgaac acccccggca accgcgtcgt cgaactggag
      841 acggcgcacc gcaccgagcc gatcgtctgc ctgtcccatc tgcgctggaa tttcgtcttc
      901 cagcgcccgc agcatctcat gtcccgcttc gcgaaatcgc gcacggtgct ggtgttcgag
      961 gaacccctgc cggtagagcc gggggcgacg atgggcgtgg atctgcgggt gtgcgccgaa
     1021 agcggcgtgg tggtcgcgac gccccgcctg cccgatggac tggatggccc cagccgcaac
     1081 gccgtcatgc gccagctgct cgacgaagcg atcgcggccc agaatatcga gcgccccgtg
     1141 ctctggtact acacgcccat gatggtgccg atcgcccggc acatcgaggc cgcggcggtc
     1201 gtctatgact gcatggacga actggcgaat ttccgttttg cgccgcctga actcacgacg
     1261 ctggagcggg aactgttcgc ttcggcggat gtggtcttca ccggcggcta cagcctctac
     1321 gaggccaagc gggcgttgca cgacaacatc catcccttcc cgtccagcgt cgatacggcc
     1381 cacttcgcgc aggcccgttc aacaccggtc ggtcgcgacg accggcgtcc gcggctcggc
     1441 ttcttcgggg tcatcgacga acggatggat ctggacctga tttccagggt cgccgaggct
     1501 cgcccggact gggacttcga aatggtgggg ccggtggtga agatcgccgc cgaggacctg
     1561 ccgcagcgcc ccaacctcag ctatccgggc cagaagtcct atgacgagct gcccgcctgt
     1621 ctcgcccggt gggacgtggc cctgatgccg ttcgcgatca atgaatcgac ccgcttcatc
     1681 agcccgacca agacgccgga atatctcgcg gcgggccggc cggtcgtttc cacgccgatc
     1741 cgcgacgtgg tccggcacta cagcgagttg gacgccgtgc ggatcgccgc cacgcccgac
     1801 ctgttcgtcg ccggctgcga ggc
//
LOCUS       DASVVN010000533         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_73434, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000533 DASVVN010000000
VERSION     DASVVN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73434"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="ssb"
                     /locus_tag="VF686_08630"
     CDS             complement(<1..340)
                     /gene="ssb"
                     /locus_tag="VF686_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8661920.1"
                     /translation="MAGSVNKVILVGNLGKDPEIRTLNSGERVANLSLATSEQWRDKA
                     TGERKEKTEWHRVVIFNENIVKVAENYLKKGSTVYIEGSLQTRKYEQNGVEKYSTEIV
                     LQKFRGELTML"
     gene            complement(503..943)
                     /locus_tag="VF686_08635"
     CDS             complement(503..943)
                     /locus_tag="VF686_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661921.1"
                     /translation="MKLLTIAALGAGALALAACEKTTEAPFDRGVCYVVELKAEGEPP
                     QFNKLAEDQPQIEFCAARLEEMRLRFLRMGGSRREIMGSYQGQFIFIDRAGVWIGQTL
                     EGSRFILLARTGDGRLAIPSAIQRAIDGTPVSVSANPEAGAPTP"
     gene            complement(943..1608)
                     /locus_tag="VF686_08640"
     CDS             complement(943..1608)
                     /locus_tag="VF686_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661922.1"
                     /translation="MPQDNRLLIAIGAGLAIVAAVVLALVFSSGRGDNGPPPAAQGGL
                     TVDVADAPELNTTRELRCFVNGQFIGMATLSDCARRNGLATDALDVGIDETGALAAAP
                     TASLAPPPSLPPVELTDVEPPVVADEPSQPQPTIQAASGSVCLRHTGSDWRSLSSNMS
                     LNACVQALYAGTCVRPGEALYGRWGETTLRLVPRRVEQSPDNNRFRTLVEQDRGCQFP
                     GLR"
     gene            complement(1673..>2011)
                     /locus_tag="VF686_08645"
     CDS             complement(1673..>2011)
                     /locus_tag="VF686_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8661923.1"
                     /translation="RRNGLATDALDVGIDETGALAGLGLSPGYIGSSVGDDVVKTFGL
                     GLPSNLTIADAVSIKTAGEKLQAAMKAVRDAYRALDPATTAPAANGPVPAYLTNQLAN
                     YQAALARLGG"
BASE COUNT          285 a          665 c          717 g          344 t
ORIGIN      
        1 ccagcatggt cagttcgccg cggaatttct gcagcacgat ttccgtcgag tatttctcga
       61 cgccgttctg ctcgtatttg cgggtctgca ggctgccctc gatgtaaacg gtcgagccct
      121 tcttcagata gttctccgcc accttgacga tgttctcgtt gaagatgacg acgcggtgcc
      181 actcggtctt ttccttgcgc tcgccggtgg ccttgtcgcg ccactgttcc gaggtggcca
      241 gagacaggtt cgcgacccgc tcgccggagt tcagggtgcg gatctccggg tccttgccca
      301 ggttgccgac cagaatgacc ttgttgacgc tgcccgccat cgccgagctc cttcttcttc
      361 gttcgggccc gccgcgcttc gcgctgttcc gggccgggat tgtttgtcag gcctgactag
      421 cgcgcttccg ccgcccgcag gtcaggtttg tggccatcat ccacaggcgg tttgttccac
      481 gtttgttccg ctccgtcgag acctaaggcg tcggggcccc cgcctcgggg ttggccgaga
      541 ccgagaccgg cgtgccgtcg atggcgcgct ggatggcgct ggggatcgcc agccgcccgt
      601 cgccggtccg ggccagcagg atgaagcgcg agccttccag cgtctgcccg atccataccc
      661 cggcgcggtc gatgaagatg aactgcccct ggtaggagcc catgatctcg cggcggctgc
      721 cgcccatgcg caggaagcgc aggcgcatct cctcgagacg ggcggcgcag aattcgatct
      781 gcggctggtc ctcagcgagc ttgttgaact gcgggggttc gccctccgcc ttcagctcga
      841 cgacgtagca gacgccccgg tcgaaggggg cctccgtggt cttctcgcag gctgcgaggg
      901 cgagggcgcc ggcgcccagc gcagcgatcg taaggagctt catcagcgca gacccgggaa
      961 ttggcagccc cggtcctgct cgaccagggt gcggaagcgg ttgttgtcgg gcgactgttc
     1021 gacccggcgc ggcacgaggc gcagcgtggt ctcgccccag cgaccgtaca gcgcctcgcc
     1081 cggacggacg caggtgccgg cgtacagcgc ctggacgcag gcgttcaggc tcatgttcga
     1141 ggacagcgag cgccagtccg aacccgtgtg gcgcaggcag acgctgccgg acgcggcctg
     1201 gatcgtcggc tggggctggc tcggctcgtc agcgaccacg ggcggctcga cgtcggtcag
     1261 ctcgaccggc ggcaggctgg gcggcggggc gagagacgcc gtcggcgcgg cggccagggc
     1321 gccggtttca tcgatgccca cgtcgagggc gtcggtggcc aggccgttgc ggcgggcgca
     1381 gtcggacagg gtggccatgc cgatgaactg gccgttgacg aaacagcgca gctcgcgcgt
     1441 cgtattcagt tcgggcgcgt cggcgacatc gaccgtgaga ccgccctgtg cagcgggcgg
     1501 cgggccgtta tcccctcgcc cgctgctgaa aacgagggcg agcacgacgg ccgcgacgat
     1561 cgccaggccg gcgccgatcg cgatgagaag acgattgtcc tggggcatga agggctcgcg
     1621 cggatggagc ccccaataac gccgccgaag cccgcgccgt caacggcctt cgtcagccgc
     1681 caaggcgggc gagcgcggcc tgatagttgg ccagctgatt ggtcagatag gccggcacgg
     1741 gtccgttggc ggccggggcg gtcgtggcgg gatccagcgc gcgataggcg tcccgcaccg
     1801 ccttcatggc cgcctgaagc ttctctccgg ccgtcttgat cgagacggcg tcagcaatcg
     1861 tcaggttgga gggcagcccc agaccgaaag tcttgacgac gtcgtcgccg acggaggatc
     1921 cgatatagcc cggcgacagg cccaggccgg ccagggcgcc ggtctcgtcg atgcccacgt
     1981 cgagggcgtc ggtggccagg ccgttgcggc g
//
LOCUS       DASVVN010000534         6188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_441972,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000534 DASVVN010000000
VERSION     DASVVN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441972"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..758
                     /locus_tag="VF686_08650"
     CDS             39..758
                     /locus_tag="VF686_08650"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX8661924.1"
                     /translation="MIRLHVPQPLSPDAAVAPTLDQSRYLTQVMRLKIGDSLLVFNGV
                     DGEWRCVIAEVLKKGVVLRAEEQVRPQSAGPDLELLISVVKKSALEFAVEKAVELGVQ
                     RIRLVVTHRTQTQHVRMDRLDAIAIEAAEQTGRLDVPMIDEPVKLAELLDGLEPGRRL
                     MFCDETGGEPAGVALQAAGEGPWAVLIGPEGGFSPEERERLRSLPFTTAVSLGPRILR
                     ADTAAIAALTLWQAAIGDWDR"
     gene            856..2217
                     /locus_tag="VF686_08655"
     CDS             856..2217
                     /locus_tag="VF686_08655"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX8661925.1"
                     /translation="MADAEPLSREDLIGAMSKGAKPKDQWRIGAEHEKFGFDRTTLRR
                     PAYEGENGILAMLTGLQRFGWTPLEEAGHVIALERRNPDGTVASISLEPGGQFELSGA
                     PLKDVHEICDETGRHLMEVKQVADQINLGFIGAGFDPMWKREDVPVMPKGRYGIMRAY
                     MPKKGSLGLDMMLRTCTIQSNLDFADEADMVAKFRTSLALQPIATALFACSPFIEGKP
                     SGFLSSRANVWTDTDPDRTGMLDFVFADGFGYERYADYALDVPMYFAKRGETYVDLSG
                     QSFRRFMTEGLDALPGERATKKDWNDHLTTLFPEVRLKSYLEMRGADGGPWSRICALQ
                     ALWAGVLYDGAALAAAWDLVKDWDIADHERLRRDVTRLGLKAEVAGRSVRDVAVDMVH
                     IAREGLKSRARFSGGMVDERGYLSELEDIADSGVTPADRLLELYHGEWAGDVSRIYRD
                     FAY"
     gene            2260..2697
                     /locus_tag="VF686_08660"
     CDS             2260..2697
                     /locus_tag="VF686_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661926.1"
                     /translation="MRSVLARIADRRDVREIAAAVAIGVVCLIAGLWWLTDDLNSAGR
                     AARAAVRDALGDPELNVGKPDIRPHPTINGERSHLTLVCGRIRGDPERPFAALVKEHR
                     RRDDALSWFPGTGDRVEQLALFRKGPLTPDQTALLAACDAGGL"
     gene            complement(2756..3079)
                     /locus_tag="VF686_08665"
     CDS             complement(2756..3079)
                     /locus_tag="VF686_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661927.1"
                     /translation="MLVLSLMAALAVQTAAPAPSGAAATLLQPREIQLNLDGRNWNCG
                     PDGACAGRGGGAPQPLMRECRRFVGRMGPVSTYARAGLALTEAEVAQCNAADIARSRR
                     NGGPA"
     gene            3183..3761
                     /locus_tag="VF686_08670"
     CDS             3183..3761
                     /locus_tag="VF686_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8661928.1"
                     /translation="MPPKGEATHARLINEAMTQVTVRGLAAVSLADVAGAASVSKSAV
                     LKHFQSKEALQAEVVDAMIERFKTAVWDPAEPLEPGRARLDRIFQGELDWIDGEDRPG
                     GCPLKAAAMELDDQPGPLRDTLKGSQIRWAKTLKREFRALYPEADEAALEVLVFQFKG
                     IVLAYGHSRRLLDDDTARAQATAAYRMLVRAA"
     gene            3785..4624
                     /locus_tag="VF686_08675"
     CDS             3785..4624
                     /locus_tag="VF686_08675"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX8661929.1"
                     /translation="MTPSSLEKPMRAAIPTIPALLAAALWLLFAPGPAEAQSFYCDDD
                     SKRVETLICADENLSALDTEMSEAYLAVIDVAADPAGLVRSQRAWLRTRNACGDRACL
                     AAAYDQRRPVVLATPRAGMATHRDPVLGLAFEYLANRRVEPCPEGHSEGPCTAVFGYR
                     GGWPNVHLASFQVFDGTLEAIAQREAGFEPRDGGWFTTYGRFEPQRVELFRSGALSGM
                     RASVICGIEDETGFHAAGGACFWGVLSDGRRSILVTAEGAEGAPPDEIANELAATLQL
                     IQP"
     gene            4635..5387
                     /locus_tag="VF686_08680"
     CDS             4635..5387
                     /locus_tag="VF686_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8661930.1"
                     /translation="MTGTIRVGIGGWTYEPWRGVFYPEKWSQKRELEYASRHTTSIEI
                     NGTYYSGFKPETWMKWRDETPDDFVFSVKASRFCTNRKVLSEGNDSLERFLDQGLTAL
                     GDKLGPINWQFMATKKFDPVDFEGFLKLLPKEKDGVRLRHALEVRSGTFDNEQFYDLA
                     RKYGAAIVYGHDDEAPQWPRIDQDTADFKYARLMSSREDEPTGMSAAELDGIVKQTRD
                     WARKGDVFAYFIAGAKVRNPAAAQALIAKLGQ"
     gene            5384..5752
                     /locus_tag="VF686_08685"
     CDS             5384..5752
                     /locus_tag="VF686_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8661931.1"
                     /translation="MTTRVHGSCHCGAQRFCAPAPESVTQCNCSICSKRGALAAYYAP
                     EQVELSLSPDLAADYQWGDRMMTFHHCTACGCSVFAEAPAWTTDEGEDRPARIVLNAR
                     LFDDLDLSVVPVRHVDGRNA"
     gene            5815..>6188
                     /locus_tag="VF686_08690"
     CDS             5815..>6188
                     /locus_tag="VF686_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8661932.1"
                     /translation="MPIATRAFAATSSDAPLSPYSFDRRDPGPDDVAIEIKFAGVCHS
                     DLHVVKNDLGNTTYPIVPGHEIAGVVTAVGDNVARFKVGDRVGVGCMVDSCRQCAPCR
                     EGEEQYCVPGMTQTYGSADPKGA"
BASE COUNT         1041 a         2026 c         2172 g          949 t
ORIGIN      
        1 aggatgacgg cggagatagg ggagcataga aaacgaccat gatccgcctc cacgtccccc
       61 agccgctctc cccggacgcc gccgtggcgc cgaccctcga ccaatcgcgc tacctgacgc
      121 aggtcatgcg gctgaagatc ggcgacagcc tgctggtgtt caacggcgtc gacggcgagt
      181 ggcgctgcgt catcgccgag gtgctcaaga agggcgtggt gctgcgcgcc gaggagcagg
      241 tgcggccgca atccgccggt cccgatctgg aactgctgat cagcgtggtg aagaagagcg
      301 cgctggagtt cgcggtcgaa aaggccgtcg aactgggcgt gcagcgcatc cggctggtcg
      361 tcacccaccg gacccagacc cagcatgtgc gcatggaccg gctggacgcc atcgccatcg
      421 aggccgccga acagaccgga cggctcgacg tgccgatgat cgacgagccg gtgaagctgg
      481 ccgagttgct ggacggtctg gagcctgggc gccggctgat gttctgcgac gagacgggcg
      541 gcgaaccggc gggcgtcgcc ttgcaggccg cgggcgaggg tccgtgggcc gtcctgatcg
      601 gtcccgaggg cggattttcc ccggaagagc gggaacggct gcggtcgctg cccttcacca
      661 ccgccgtatc gctggggccg cgaatcctgc gggccgacac tgccgcgatc gccgccctga
      721 ccctgtggca ggcggccatc ggcgactggg atcgataagc cccaaggcgg ccttatccat
      781 gccggagcag aatgggactt gagccagccg ctccgtctgc ccatctagcc gcgacaagag
      841 gggttacaga tccgcatggc cgacgccgaa ccgctttccc gggaagacct gatcggcgcc
      901 atgtcgaaag gggccaaacc caaggatcaa tggcgcatcg gcgccgagca cgagaagttc
      961 ggtttcgacc gcaccaccct gcgccgcccg gcctatgagg gcgaaaacgg catcctggcc
     1021 atgctcaccg gcctgcagcg gttcggctgg acgccgctcg aggaggcggg ccacgtcatc
     1081 gccctggagc gccgcaatcc cgacgggacc gtcgcctcca tcagcctcga gcccggcgga
     1141 cagttcgaac tctcgggcgc gccgctgaag gacgtgcacg aaatctgcga cgagaccggc
     1201 cgccacctga tggaggtcaa acaggtcgcc gaccagatca acctcggctt catcggcgcc
     1261 ggcttcgacc cgatgtggaa gcgcgaggac gtgccggtca tgcccaaggg ccgctacggc
     1321 atcatgcggg cctatatgcc gaagaagggg tcgctgggcc tcgacatgat gctgcgcacc
     1381 tgcaccatcc agtcgaacct ggacttcgcg gacgaggccg acatggtcgc caaattccgc
     1441 acctctctgg cgctgcagcc gatcgccacg gccctgttcg cctgctcgcc cttcatcgag
     1501 ggcaagccca gcggcttcct gtcgtcgcgg gccaatgtct ggaccgacac ggatccggac
     1561 cggacgggca tgctggactt cgtcttcgcg gatgggttcg gctacgagcg ctacgccgac
     1621 tatgcgctgg acgtgccgat gtatttcgcc aagcgcggcg agacctatgt cgacctgtcg
     1681 gggcagtcgt tccgccgctt catgaccgag ggcctcgacg ccctgcccgg cgagcgggcg
     1741 acgaagaagg actggaacga ccacctgacc acgcttttcc ccgaggtgcg gctgaagtcg
     1801 tatctggaga tgcgcggcgc cgacggcgga ccgtggagcc ggatctgcgc gctgcaggcc
     1861 ctgtgggccg gcgtgctgta cgacggtgcg gccctggccg cggcctggga cctggtcaag
     1921 gactgggaca tcgccgacca cgaacgcctg cgccgcgacg tgacgcgtct gggtctgaag
     1981 gccgaggtgg cgggccggtc ggtgcgcgac gtggccgtgg acatggtcca catcgcccgc
     2041 gaggggctga agagccgcgc ccgcttctcg ggcggcatgg tcgacgagcg cggctatctg
     2101 tcagagctgg aagacattgc cgacagcggg gtcacccccg ccgacaggct gctggagctt
     2161 tatcacggcg agtgggccgg cgacgtgagc cggatctacc gggacttcgc ctactaggcg
     2221 gctacggttt cttagggccg ccgcgccagg ctgcgggtca tgagatcagt cttggcccgg
     2281 atcgccgacc ggcgcgatgt ccgcgagatc gcggctgcgg tggccatagg ggtcgtgtgc
     2341 ctgatcgccg ggctgtggtg gttgaccgac gacctgaact ctgccggacg ggcggcgcgg
     2401 gcggcggtgc gtgacgctct cggcgacccg gagctgaacg tcgggaagcc ggacatccgc
     2461 ccccatccga ccatcaatgg agagcggagc cacctcacac tggtctgcgg tcgcatcagg
     2521 ggagacccgg aacgcccgtt cgccgcgctg gtgaaggaac accgccgccg cgatgacgcg
     2581 ttgagctggt ttcccggaac cggcgaccgg gtcgaacaac tggctctgtt ccggaagggg
     2641 ccgttgacgc ccgatcagac agcattgctg gctgcatgcg acgccggagg cctgtagccg
     2701 aacggcggcc gctcaccgtc ccgccggatg acgggacggt gtgaggcgca tcgcctcagg
     2761 cgggaccgcc gttgcgacgg gaccgggcga tgtcggccgc gttgcactgg gcgacctcgg
     2821 cttccgtcag cgccaggccg gcgcgggcgt aggtcgagac cgggcccatg cggccgacga
     2881 accggcggca ttcacgcatc aacggctggg gcgcgccgcc gccgcggccg gcgcaggcgc
     2941 cgtcgggacc gcagttccag ttgcgtccat ccaggttcaa ctggatttcg cggggctgca
     3001 gaagggtggc cgcagcgccc gagggagcgg gggcggcggt ctggacggcg agggccgcca
     3061 taagggacag tacgagcatg gtatcctccg gggctggtca gaacgaacgg ttccgtgtaa
     3121 aagtgaccgt tcgtgcaccg ataagtgacc gatcggttac ttcgagtcaa gaggatttct
     3181 gaatgccgcc caaaggcgag gccacacacg cccgactgat caacgaggcg atgacgcagg
     3241 tcaccgtgcg cggtctcgcg gcggtcagtc tggcggatgt ggcgggcgcg gcgagcgtgt
     3301 ccaagagcgc ggtgctgaag catttccagt ccaaggaagc gctgcaggcc gaggtggtcg
     3361 acgccatgat cgagcgcttc aagaccgccg tctgggatcc cgccgagccg ctggagccag
     3421 gccgcgcgcg gctggaccgt atcttccagg gcgaactgga ctggatcgac ggcgaggacc
     3481 gccccggcgg ctgtccgctg aaggccgcgg ccatggagct ggacgaccag cccggcccgc
     3541 tgcgtgacac gctgaagggc agccagatcc gttgggcgaa gacgctgaag cgcgagttcc
     3601 gggccctgta tcccgaggcg gacgaggcgg cgctcgaggt gctggtattt cagttcaagg
     3661 gcatcgttct ggcctatgga cattcccgcc ggctgctgga cgacgacacg gcccgcgccc
     3721 aggcgacggc ggcataccgg atgcttgtca gggcggcttg atcgccgggc ccggacggtg
     3781 gagggtgacg ccatcctcgc tggagaagcc gatgcgcgcc gccatcccga cgatcccggc
     3841 gctcctcgca gccgcgctgt ggctgctgtt cgcgcccggc ccggccgagg cccagagctt
     3901 ctactgcgat gatgacagca aacgggtcga gaccctgatc tgcgccgacg agaacctgag
     3961 tgcgctcgat accgaaatgt cggaggcgta tctcgcggtg atcgatgtgg ccgcagaccc
     4021 ggccggtctc gtgcggtcgc agcgtgcctg gctccggacg cgcaacgcct gcggggaccg
     4081 ggcctgtttg gccgccgcat atgaccagcg gagaccggtc gtactggcca cgccccgggc
     4141 cgggatggcg acccatcgcg acccggtgct ggggctggcg ttcgaatatc tggcaaatcg
     4201 ccgggtggag ccttgcccgg aagggcattc cgagggcccc tgcacagccg tctttggata
     4261 ccgcggcggc tggccgaacg tccatctggc ctccttccag gtattcgacg gaacgctgga
     4321 agcaatcgcg cagcgcgagg ccgggttcga gccgcgcgac gggggctggt tcaccaccta
     4381 tggccgcttt gaaccgcagc gggtggagct gttccgaagc ggcgccctgt ccggcatgcg
     4441 ggcctcggtg atatgcggaa tcgaggacga gaccggcttc cacgccgccg ggggcgcgtg
     4501 tttctggggc gtgctgagcg acggccggcg ttcgattctc gtcaccgccg agggcgccga
     4561 gggagctccg ccggacgaga tcgccaacga gcttgcagcc acactgcaat tgatccagcc
     4621 ctgaggagac ctgcatgacc ggaaccattc gcgtaggcat cggcggctgg acctatgagc
     4681 cgtggcgcgg cgtcttctac cccgagaaat ggagccagaa gcgcgagctg gaatacgcca
     4741 gtcgccacac gacctcgatc gagatcaacg gcacctacta ttcgggcttc aaacccgaaa
     4801 cctggatgaa gtggcgcgac gagacgcccg acgatttcgt cttttcggtc aaggccagcc
     4861 ggttctgcac caaccgcaag gtgctgtcgg agggcaacga cagccttgaa cggtttctcg
     4921 atcaggggct gacggcgctg ggcgacaagc tggggccgat aaactggcag ttcatggcca
     4981 ccaagaagtt cgaccccgtc gacttcgagg gcttcctgaa gctgttgccg aaggagaagg
     5041 acggggtgcg tctgcgccat gccctggagg tccgcagcgg caccttcgac aacgagcagt
     5101 tctacgacct ggcccgaaaa tacggcgcgg ccatcgtcta cgggcacgat gacgaggccc
     5161 cgcagtggcc ccggatcgat caggacacgg ccgacttcaa atacgcgcgc ctgatgtcca
     5221 gccgcgagga cgaacccacg ggcatgtctg cggccgaact ggacggcatc gtcaaacaga
     5281 cccgcgactg ggctaggaag ggcgacgtgt tcgcctattt catcgccgga gcgaaggtgc
     5341 gcaatccggc ggcggcgcag gcgctgatcg cgaaactggg ccagtgacga cgcgggtgca
     5401 cggcagttgc cactgcgggg cgcagcggtt ctgcgcgccc gcgccggaga gcgtgacgca
     5461 gtgcaactgc tccatctgtt cgaagcgcgg cgcgctggcg gcctattatg cgccggaaca
     5521 ggtcgagctc agcctctcgc cggatctggc cgccgactac cagtggggcg accggatgat
     5581 gacctttcat cattgtacgg cctgcggatg cagcgtcttc gctgaggcgc ccgcctggac
     5641 cacggacgag ggcgaggacc ggcccgcgcg catcgttttg aacgcccgcc tgtttgacga
     5701 tctggacctg tcagtcgtcc ccgtgcggca tgtcgacggg cgcaacgcct gacagccttg
     5761 cggattgacc ccggcgtaag cacctgcccc tgaagccagt ctcgaggacc ctccatgccc
     5821 atcgccaccc gcgccttcgc cgccacgtcc agcgacgctc ccctgtcgcc ctacagcttc
     5881 gaccggcgcg atccgggtcc ggacgatgtg gccatcgaga tcaaattcgc gggcgtctgc
     5941 cactcggacc ttcacgtggt gaagaacgat ctgggcaata cgacctatcc gatcgtgccg
     6001 ggtcacgaga tcgcgggcgt ggtgacggcg gtgggcgaca acgtcgcccg cttcaaggtc
     6061 ggcgatcggg tcggcgtcgg ctgtatggtc gacagctgcc gccagtgcgc gccctgccgc
     6121 gagggcgagg agcagtactg cgtccccggc atgacccaga cctacggctc ggccgacccc
     6181 aagggcgc
//
LOCUS       DASVVN010000535         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_98611, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000535 DASVVN010000000
VERSION     DASVVN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98611"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VF686_08695"
     CDS             complement(<1..483)
                     /locus_tag="VF686_08695"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505792.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEX8661933.1"
                     /translation="MCGIIGVTGVGPAVPRLIDSLKRLEYRGYDSAGVAALVGGRIER
                     RRAKGKIRELEAVLAAEPLDATIGIGHTRWATHGAPTTANAHPHKAGRVVLVHNGIIE
                     NFAELKAELAADGHVFESQTDTEIVAHLLDRELGRANDPLVAFKTVLDRLTGAYALAV
                     L"
     gene            650..1999
                     /gene="glmM"
                     /locus_tag="VF686_08700"
     CDS             650..1999
                     /gene="glmM"
                     /locus_tag="VF686_08700"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8661934.1"
                     /translation="MGERRYFGTDGIRGRANSHPMTPEVALRVGLAAGRMFMSSDDRR
                     HLVVIGKDTRLSGYMIEPALVAGFASAGMDVLTFGPLPTPGVAMMTRSMRADIGVMIS
                     ASHNAYLDNGIKLFGPDGYKLSDEVELKIESMMDEPLDAGLAPPDKLGRVKRIDDSQA
                     RYIEIAKASFPKGLSLQGLRIAIDCANGAGYKVAPTTLYELGAEVFPVGVEPNGLNIN
                     ANCGSTYPQTLAEAVKTHGAHIGIALDGDADRVIICDEKGQIVDGDQIMTMVALDWAK
                     RGLLVGGGVVATVMSNLGMERALNAAGLTLERTKVGDRYVMERMRQGGFNMGGEQSGH
                     IILHDYATTGDGLMSALQVLAVLVESGRSMSDLARQFEPVPQLLENVRFAAGKPLQDA
                     GVKAAIADAEARLVGAGRLLVRPSGTEKLIRVMAEGDDAELVKSVVADVVAAVKAAG"
     gene            complement(1996..>2629)
                     /locus_tag="VF686_08705"
     CDS             complement(1996..>2629)
                     /locus_tag="VF686_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8661935.1"
                     /translation="APPTDRLLKGPPSRRLAEARRLVSETGSWRSVMEGLRPWTSQLW
                     TEAEPKTRARWLRHLRPWWDVHRHRIPPEIDEAMDRLEAAGRLDVSAGRVDRIEAETD
                     DVTVHWRPRDGTQQPPLTAAWLIDCTGPGHAAAQTPLTSALLAEGRVRLEPLGLGLDV
                     AADGRAIRADGTPDDRLFVLGPPAFASFWETTAVPDIRKRIEGLAGALGG"
BASE COUNT          405 a          878 c          931 g          415 t
ORIGIN      
        1 cagcacggcc agggcatagg cgccggtcag gcgatcgagc acggtcttga acgccaccag
       61 cggatcgttg gcccgaccca gttcgcggtc cagcaggtgg gcgacgatct ccgtgtcggt
      121 ctggctttcg aacacatggc cgtccgcggc cagttcggcc ttcagctcgg cgaaattctc
      181 gatgatgccg ttgtggacca gaacgacgcg gccggccttg tgcgggtggg cgtttgcggt
      241 cgtgggcgcg ccgtgggtgg cccagcgcgt gtggccgatg ccgatcgtgg cgtccagcgg
      301 ctcggcggcg agcaccgctt ccagttctct gatcttgccc ttggcgcggc gtcgttcgat
      361 acgtccgcca acaagagcgg cgacgcccgc cgagtcatag ccccggtatt ccagccgctt
      421 caggctgtcg atcagacggg gaaccgccgg gccgacgccc gtcacaccga tgatgccgca
      481 catggataag tccctcggat gtctgccttt ccacgctttg cgcgcggacg ggtaacggtc
      541 taagccgggc ccggctgtct ggcgtgggtg taggcgacag cggccccgat ttgcacctaa
      601 aaagcggttt cgcaaggttt ctgcgactgc gacgagagga agacgaccct tgggcgagag
      661 acgctatttc ggcaccgacg gcatccgcgg acgcgccaac agccacccga tgacgcccga
      721 agtggccctg cgggtcgggc tggcggcggg gcggatgttc atgtcgtccg atgaccgccg
      781 gcacctcgtg gtgatcggca aggacacccg tctgtcgggc tatatgatcg aacccgcgct
      841 ggtcgcaggc ttcgcctcgg ccggcatgga cgtgctgacc ttcggccccc tgccgacgcc
      901 cggcgtggcc atgatgaccc ggtccatgcg ggccgacatc ggggtgatga tctcggcctc
      961 gcacaacgcc tatctcgaca acggcatcaa gctgttcggt cccgacggct acaagctgtc
     1021 ggacgaggtc gagctgaaga tcgaatcgat gatggacgag cccctggatg cgggcctcgc
     1081 gccgcccgac aagctgggcc gggtcaagcg gatcgacgac agccaggccc gctatatcga
     1141 gatcgccaag gccagcttcc ccaagggcct gagcctgcag ggtctgcgca tcgccatcga
     1201 ttgcgccaac ggcgccggct acaaggtggc gccgacgacg ctgtacgaac tgggcgcgga
     1261 agtgtttccc gtcggagtcg agccgaacgg gctgaacatc aacgccaact gcggctcgac
     1321 ctatccccag acgctggccg aggcggtgaa gacccatggg gcccacattg gcatcgccct
     1381 ggacggcgac gccgaccggg tcatcatctg cgatgagaaa ggtcagatcg tcgacggcga
     1441 ccagatcatg accatggtgg cgctggactg ggcgaagcgg ggtctgctgg tcggcggcgg
     1501 cgtggtggcg acggtcatgt ccaacctggg catggagcgc gcgctcaacg cggccggcct
     1561 gacgctggag cggaccaagg tcggcgaccg ctatgtcatg gagcggatgc gccagggcgg
     1621 gttcaacatg ggcggggaac agtccggcca catcatcctg catgactacg ccaccaccgg
     1681 cgacggcctg atgtcggccc tgcaggtgct ggcggttctg gtcgaaagcg gccgctccat
     1741 gagcgatctg gcccgtcagt tcgagccggt gccgcagttg ctggagaatg tccgtttcgc
     1801 cgccggcaag ccgctgcagg acgccggggt caaggcggcc atcgccgacg ccgaggcgcg
     1861 tctggttggg gccgggcgcc tgctggtgcg gccttcgggg accgagaagc tgatccgggt
     1921 gatggccgag ggcgacgatg cggagctggt gaaatcggtg gtggccgatg tggtcgcagc
     1981 ggtgaaggcg gcgggctagc cgcccagcgc ccctgccagt ccttcgatcc gcttgcggat
     2041 gtccggtacg gccgtcgttt cccagaagga cgcgaacgcc ggcggcccca gcacgaagag
     2101 ccggtcgtcc ggcgtcccgt cagcccggat cgcccgcccg tccgcagcca cgtccagccc
     2161 gaggcccagg ggttcgagcc gcacacggcc ttcggcgagc agggcggacg tgaggggggt
     2221 ctgagccgcc gcatgcccgg ggcccgtgca gtcgatcagc caggccgcgg tcaggggcgg
     2281 ttgctgcgta ccgtcccttg gccgccagtg caccgtgacg tcgtccgttt ccgcctcgat
     2341 ccggtcgacc cgaccggccg agacatcgag gcggcccgcg gcctccagcc tgtccatcgc
     2401 ctcgtcgatc tccggcggga tccgatggcg gtgcacgtcc caccacggcc gcaaatggcg
     2461 cagccagcgg gcgcgggtct tcggctccgc ctccgtccag agctgtgagg tccagggccg
     2521 aagcccctcc atcacactgc gccagctgcc agtctcgctc accagccgtc gggcctcggc
     2581 gaggcgccgc gacggcggtc ctttcagcag gcggtcggtc ggcggggcc
//
LOCUS       DASVVN010000536         5798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_467358,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000536 DASVVN010000000
VERSION     DASVVN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467358"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="lgt"
                     /locus_tag="VF686_08710"
     CDS             <1..674
                     /gene="lgt"
                     /locus_tag="VF686_08710"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX8661936.1"
                     /translation="LGIILGGRLGYALFYQPSLYGQLFTGADWGERLSLLRLWDGGMS
                     FHGGLIGVSIAIVWFAWRHKVRLLSLGDLVAPAVPIGLFFGRVANFINGELWGRETTV
                     PWAVRFCNDRIYRLYNGCPAGDVPRHPSQLYEAGLEGLILFAILWFAVHKAKLLAKPG
                     YVTGIFLFGYGLSRALLENVRQPDAGLDNLPLGLTMGMILSIPMMIIGALLIWRAWSR
                     PPVVV"
     gene            674..1747
                     /locus_tag="VF686_08715"
     CDS             674..1747
                     /locus_tag="VF686_08715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8661937.1"
                     /translation="MSLADRLAREIALTGPMTIADYVARCLHDPEGGYYATRPAIGAT
                     GDFVTAPMISQMFGELIGLWAVELWQRLGAPERIRLVEVGPGDGTLMADALRAARLAP
                     DFLAAVDLVLIEPSPPLRAAQARMLNDSDVHPRWVTDLNSVETDAPVILIANEVLDCL
                     PARQFVKTVDGWAERRIGVTDEGGLTFGLVRITGGFTRPAFEVEPGQTVEISDQQAAF
                     GRDVATLIKAASGAALLIDYGRARPEAGDTLQALRRHQKVDPLETPGEADLTQWADFP
                     TVQEAAIRAGADVTGAVGQGEFLRLLGIEARADRLKAGRPDAAPVIERQLARLTADDQ
                     MGALFKACAVFSPRTLIVPGFED"
     gene            1890..2663
                     /gene="pgeF"
                     /locus_tag="VF686_08720"
     CDS             1890..2663
                     /gene="pgeF"
                     /locus_tag="VF686_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX8661938.1"
                     /translation="MTDLAPITHPLLDKAGVRHGFFTRQGGVSTGIYDSLNTGLGSTD
                     DPSAVAQNRQRVAAHMGGDPDDFAACYQIHSSLARVAVAGWKGERPEGDASVTAAPGV
                     ICAVLTADCAPVLLADAEAGIVGAAHAGWKGALGGIVSSAVASMEALGAEPRRMVAVV
                     GPCIAQASYEVGADFQERFDHHDPGGARFFAPGATSDKHLFDLPGFVLWRLEQAGVGE
                     AAWTGHDTRVDEAQFFSNRRAYLNGEPDFGRMMSAISLR"
     gene            complement(2676..3575)
                     /gene="lpxC"
                     /locus_tag="VF686_08725"
     CDS             complement(2676..3575)
                     /gene="lpxC"
                     /locus_tag="VF686_08725"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX8661939.1"
                     /translation="MPVSYDHRQKTTVAPAIIAGVGVHTGDRVRLAVRPAPVGTGIVF
                     VRTDIKDRDNRIPVSGDAVVDARLNTMIENAAGVRLSTIEHLMAAFAALGVSNAVVEV
                     DGPELPILDGSALQFVQLLDRAGFRRQEAAVRYIEILEPVRVQEGDKSAALLPCDRYE
                     MRFEIDFDTPVIGNQVVDFVVDEETFRNEIMAARTFGFAHEVEALRRAGLARGGSLEN
                     AVVIDGDQILNPGGLRMEREFVKHKALDAIGDLYVLGAPLLGRYEGVKAGHAINNLLV
                     RELLANPQAWREVTGAPELAMAG"
     gene            complement(3765..5372)
                     /gene="ftsZ"
                     /locus_tag="VF686_08730"
     CDS             complement(3765..5372)
                     /gene="ftsZ"
                     /locus_tag="VF686_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8661940.1"
                     /translation="MSLSLVRPQHTELKPRIVVFGVGGAGGNAVNNMIDAGLEGVEFV
                     VANTDAQHLSFAKTDRRVQLGETITQGLGAGAHPEVGMNAAEESAEEIYGHLDGAHMV
                     FITAGMGGGTGTGAAPVIAKCARDRGILTVGVVTKPFTFEGRHRMRLADAGIAELQRY
                     VDTLIVIPNQNLFRVANERTTFADAFGMADQVLHSGVRSITDLMILPGLINLDFADVR
                     AVMSEMGKAMMGTGEASGDDRALMAAQNAIANPLLDETSLKGAKAVLVNITGGMDMTL
                     LEVDEAANAIAGEVDADANIIFGAAFDPALDGKIRVSVVATGMEDLAAAKGAPSTGTS
                     AFDTRRPAAPYAASRAEPVRHEPVREPARAETPRQPEPAPVVPTFQSAQPTFGTAARA
                     PEPRPEPVIHVAEDRALEPIVDPWVEEYESSPRARTAAETTAPVAQGDLYMDRSAERA
                     PEPETRREEPAYEEYDDRDHRKSGWSLFGRGKRQPAQPTYPQSRPTESRMTEMRPTTQ
                     AQPSPETQLGQAEDDLEIPSFLRRLAN"
     gene            complement(5617..>5798)
                     /locus_tag="VF686_08735"
     CDS             complement(5617..>5798)
                     /locus_tag="VF686_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661941.1"
                     /translation="NFARHDFSVRYEMTDQVTLRAGVTNAFDAEQPRILGAGLLDNFD
                     PYGTRFNIGLNWRPY"
BASE COUNT          853 a         1952 c         2025 g          968 t
ORIGIN      
        1 ccctgggcat catcctcggc ggccgcctcg gctacgccct cttctaccag ccttcgctgt
       61 acgggcagtt gttcaccggc gcggactggg gcgagcggct gtcgctgctg cgcctgtggg
      121 acggcggcat gtcgttccac ggcgggctga tcggggtctc gatcgcgatc gtctggttcg
      181 cgtggcggca caaggtgcgg ctgctcagcc tcggcgatct ggtcgccccc gccgtgccga
      241 tcggcctgtt cttcggccgc gtggccaact tcatcaacgg cgagctgtgg ggccgcgaga
      301 cgaccgtccc ctgggccgtc cgcttctgca acgaccgcat ctaccgcctc tacaatggct
      361 gcccggccgg agacgtgccg cgccacccca gccagcttta cgaggcaggt ctggaaggcc
      421 tgatcctgtt cgccatcctg tggttcgccg tgcacaaggc gaaactgctg gccaagccgg
      481 gctatgtcac gggcatcttc ctgttcggat acggcctctc gcgggcgctt ctggagaacg
      541 tgcgccagcc ggacgccggt ctggacaacc tgccgctggg cctgaccatg ggcatgatcc
      601 tgtcgatccc gatgatgatc atcggggccc tgctgatctg gcgcgcctgg agccggccgc
      661 ccgtcgtcgt ctgatgtcgc tggcggacag actggcgcgc gaaatcgccc tgacggggcc
      721 gatgacgatc gccgactacg tcgcccgctg cctgcacgac ccggaaggcg gctactacgc
      781 cacccgtccc gccatcggcg cgaccggcga cttcgtcacc gcgccgatga tcagccagat
      841 gttcggcgaa ctgatcggcc tgtgggcggt cgagctgtgg cagaggcttg gggcgcccga
      901 gcgcatccgg ctggtcgagg tcgggcccgg ggacggcacg ctgatggccg acgcgctgcg
      961 cgcggcgcgg ctggcccccg acttcctcgc cgccgtcgat ctggtgctga tcgagcccag
     1021 cccgcccctg cgcgccgccc aggcgcggat gctgaacgac agcgatgtcc atccgcgctg
     1081 ggtcacggac ctgaacagcg tcgagaccga cgcccccgtc atcctgatcg ccaatgaggt
     1141 gctggactgt ctgcccgcgc gccagttcgt caagacggta gacggctggg ccgagcgccg
     1201 catcggcgtc accgacgagg gcggcctgac cttcggcctg gtgcggatca ccggcggttt
     1261 cacccgcccc gccttcgagg tcgaacccgg ccagacggtc gagatttcgg accagcaggc
     1321 cgccttcggc cgcgacgtgg cgaccctgat caaggcggcc tcgggcgcgg cgctgctgat
     1381 cgactacggc cgcgcccgac ccgaggcagg cgacaccctt caggccctgc ggcggcatca
     1441 gaaggtcgac ccgctggaaa cgccgggcga ggccgacctg acccagtggg ccgacttccc
     1501 gactgtgcag gaggccgcca tccgcgccgg agccgacgtc accggcgcgg tcggtcaggg
     1561 tgaattcctc cggctgctgg gcatcgaagc tcgcgccgac cggttgaagg cgggacggcc
     1621 ggacgccgcg cccgtcatcg agcgccagtt ggcccgcctg accgcggacg accagatggg
     1681 ggccctgttc aaggcctgcg cggtcttttc accccgcacc ctgattgttc cgggtttcga
     1741 ggactgatcg cagtggcact gttttgcgaa ttcggggcgc caaccgtgcc aaaccgcgcc
     1801 aaaatcgttc tgctccataa cctcgcggcc ctccctgcgc atagagtagg cgggccctac
     1861 gtcaggaacg gtcgtatgga gcgcgagcaa tgaccgacct tgctcccatc actcatcccc
     1921 ttctcgacaa ggccggggtg cggcacggct tcttcacgcg gcagggcggc gtttccaccg
     1981 gcatctacga cagcctgaac accggcctcg ggtcgaccga cgatccctcc gcggtggcgc
     2041 agaaccggca gcgggtggcg gcgcatatgg gcggcgaccc cgacgacttc gccgcctgct
     2101 accagatcca ttccagcctc gcccgcgtgg cggtggccgg gtggaagggc gaacgacccg
     2161 aaggcgacgc cagcgtcacc gccgcgcccg gcgtcatttg cgctgtcctg accgccgact
     2221 gcgcgcccgt gctgctggcg gacgccgagg ccgggatcgt cggggcggcc catgcgggct
     2281 ggaagggcgc gctgggcggc atcgtctcct ccgccgtcgc ttccatggag gccctcggcg
     2341 ccgagccgcg ccgcatggtc gccgtcgtcg gcccctgcat cgcccaggcc agctatgagg
     2401 tcggcgcgga cttccaggaa cggttcgatc accacgaccc gggcggcgcc cgtttcttcg
     2461 ccccgggcgc aacgtcggac aaacacctgt tcgacctgcc gggcttcgtg ctgtggcgtc
     2521 tggagcaggc cggcgtcggc gaggccgcct ggaccggcca cgacacgcgc gtggatgagg
     2581 cgcagttctt ctcgaaccgg cgcgcctatc tcaacggaga accggacttc ggccggatga
     2641 tgagcgcgat cagcctgcgc tgatcatggg ctggttcagc ccgccatcgc cagctcaggc
     2701 gcgccggtca cttcacgcca cgcttgcgga ttggccagca gctcgcggac cagcaggttg
     2761 ttgatcgcgt gccccgcctt gacgccctcg tagcggccca gcaggggcgc gccgagcaca
     2821 tacaggtcgc cgatggcgtc cagcgccttg tgcttgacga actcgcgttc catccgcagc
     2881 ccgccgggat tcaggatctg gtcgccgtcg atgaccacgg cgttttccag cgaaccgccg
     2941 cgggccagac cggcgcggcg cagggcctcg acctcatggg cgaagccgaa ggtgcgggcg
     3001 gccatgatct cgttgcggaa ggtttcctcg tcgacgacga agtccacaac ctgattgccg
     3061 atgaccggcg tgtcgaagtc gatctcgaac cgcatctcat agcggtcgca gggcagcagg
     3121 gccgcggact tgtcgccttc ctgcacgcgg accggctcga ggatctcgat atagcggacg
     3181 gccgcttcct gacggcggaa gccggcgcgg tccagcagct ggacgaactg cagcgccgaa
     3241 ccgtcgagga tcggcagttc gggcccgtcc acctcgacca cggcgttcga cacgcccagg
     3301 gcggcgaagg cggccatcag atgctcgatg gtcgacaggc gaacgccggc ggcgttctcg
     3361 atcatggtgt tcagacgggc gtcgacgacg gcgtcgcccg agacggggat gcggttgtcg
     3421 cggtccttta tgtcggtgcg cacgaagacg atgcccgtgc cgaccggcgc cggacgcacg
     3481 gccagacgca cccggtcgcc cgtgtgcacg cccacgcccg cgatgatcgc gggggcgacg
     3541 gtggtcttct ggcgatggtc gtagctgacc ggcaaactcg aactctctgg taattacggc
     3601 gtccgccgcg cgtgcggatt acgccccagc aaccgtctaa cggcgtcgcc acaagagcga
     3661 aatgaaagtc gggtttccga ttgtttcggt ttcggtaact gtcccggaca ggaaaaagcc
     3721 ccggagcctc ggctccgggg ccttcctcaa tcagtcgaac agcctcagtt ggcgaggcgg
     3781 cgcaggaacg acgggatttc cagatcgtct tccgcctggc ccagttgcgt ttcaggcgaa
     3841 ggctgggcct gggtggtggg gcgcatctcg gtcatccggg actcggtcgg acgcgattgc
     3901 ggataggtcg gctgcgccgg ctgacgcttg ccgcggccga acaggctcca gcccgacttg
     3961 cgatgatcgc ggtcgtcgta ctcctcatac gccggctctt cgcggcgcgt ttccggttcc
     4021 ggagcgcgct cggccgagcg gtccatgtac aggtcgccct gggccaccgg agcggtcgtt
     4081 tcggcggccg tgcgggcgcg gggcgaggat tcgtactcct cgacccacgg atcgacgatc
     4141 ggctccagcg cgcggtcctc ggcgacgtgg atcacgggtt ccgggcgcgg ctcgggagcg
     4201 cgggccgcag ttccgaacgt cggctgggcg gattgaaagg tcggaacgac cggggccggc
     4261 tcgggctggc gcggggtctc ggcgcgggcg ggctcgcgaa cgggctcgtg acggacgggc
     4321 tcggcgcgcg aggcggcata gggagcagcc gggcggcggg tgtcgaaggc cgaggtcccg
     4381 gtcgagggcg cgcccttggc ggcggccaga tcttccatgc cggtggccac gaccgagacg
     4441 cggatcttgc cgtccagggc cgggtcgaag gcggcgccga agatgatgtt ggcgtcggcg
     4501 tccacctcgc cggcgatggc gttggcggcc tcgtcgactt ccagcagggt catgtccatg
     4561 ccgccggtga tgttcaccag cacggccttg gcgcccttca gcgaggtctc gtccagcagc
     4621 gggttggcga tggcgttctg ggccgccatc agggcgcggt cgtcgcccga ggcttcgccc
     4681 gtgcccatca tcgccttgcc catttcggac atgacggcgc ggacgtcggc gaaatcgagg
     4741 ttgatcaggc ccggcaggat catcaggtcg gtgatcgaac gcacgcccga atgcaggacc
     4801 tggtcggcca tgccgaaggc gtcggcgaag gtcgtccgct cattggcgac gcggaacagg
     4861 ttctggttcg ggatgacgat cagggtgtcg acgtagcgct gcagctcggc gatgccggcg
     4921 tcggccagac gcatgcggtg acggccctcg aaggtgaagg gcttggtcac gacgccgacc
     4981 gtcaggatgc cgcggtcgcg ggcgcatttg gcgatgacgg gggccgcgcc ggtgccggtg
     5041 ccgccgccca tgccggcggt gatgaagacc atgtgggcgc cgtccaggtg cccgtagatt
     5101 tcctcggcgg attcctcggc ggcgttcatg ccgacttcgg ggtgggcgcc ggcgcccagg
     5161 ccctgggtga tggtctcgcc cagctggacg cggcggtcgg tcttggcgaa cgacagatgc
     5221 tgcgcgtcgg tgttggcgac cacgaactcg accccttcga ggccggcgtc gatcatgttg
     5281 ttgacggcgt tcccgccggc tccgccgacg ccgaagacga cgatccgggg tttcagttcc
     5341 gtgtgttgcg gacgcaccag cgagagagac atattcttat ccgaccttat ccgaccctgc
     5401 ccttgcctta gccccaatgc gattccccgc cgattcgcat tggttatgag cgcgttaagg
     5461 aaacacctga actgcttaag ggagtgttac ccgataggct gagtcgggcc ggaggccacg
     5521 gatttcgtta accttcaccg cagtttttca aagagcgcca acgaaaaagg gcggctcttg
     5581 cgagccgccc ttcccgtcta cggattactc cgaagcttag tacggacgcc agttcagacc
     5641 gatgttgaag cgggtgccgt aggggtcgaa gttgtcgagc aggcccgcgc ccaggatgcg
     5701 cggttgctcg gcgtcgaagg cgttcgtcac gccggcgcgg agagtgacct ggtcggtcat
     5761 ctcgtaacgc accgagaagt cgtggcgagc gaagttgc
//
LOCUS       DASVVN010000537         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_203734,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000537 DASVVN010000000
VERSION     DASVVN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203734"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..634
                     /locus_tag="VF686_08740"
     CDS             374..634
                     /locus_tag="VF686_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661942.1"
                     /translation="MPSQSKALPHWTWDDLEAALNGFSPLGPNDPVRAHLMRGLTDDA
                     QRATPRELLQQVLSAGWVMAQVGAQSGASATAPRRAAGSSGS"
     gene            852..1268
                     /locus_tag="VF686_08745"
     CDS             852..1268
                     /locus_tag="VF686_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661943.1"
                     /translation="MTATYVAFFAAMIFGWLKGGHPERLAVAVLLLTEPVISNPLVHW
                     RIDDIYLDSTIEDALRSLFLGWLAFRSDRWWPYVATAAMALTVLVHILTVVTDISWDA
                     AVSARVGLALLMYVTLLAGVAERWMAGEAPVCRIGP"
     gene            1468..>2076
                     /locus_tag="VF686_08750"
     CDS             1468..>2076
                     /locus_tag="VF686_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8661944.1"
                     /translation="MSRSSFQFTSESVSEGHPDKVADRISDTVVDLFLGKDPYARVAC
                     ETLTTTNLVVLAGEIRGQGIMDTDGNWAPGVQDEIEAAVRAAVRDIGYEQSGFHWNKF
                     EFINRLHGQSADIAVGVDAAGEKDEGAGDQGIMFGYASNETPELMPATLQYSHNILKR
                     LAEVRHGGDKRLEPDAKSQVTIQYEDGKPVRATSIVLSTQHAA"
BASE COUNT          358 a          679 c          698 g          341 t
ORIGIN      
        1 ggtcaggtgt tcgagtcacc taagcggcac cattctccgg gcatcgacaa caggatgatc
       61 tggacgcttc gttcggaacg tcatagctag accgctgtat ccggagtggt catgacccag
      121 cggctcagtg cgcgtcagga agaatgtctg aggctgaccg cctttctgac ggacaaggaa
      181 atcgccgccc gtctcggtct gtcggaagcg accgtcaaga agcacgtaca cgagggatgc
      241 cggcgtctgg gcgtcaatcg gcgcaaggcc gcgctggcga tcctggaacg aaacgtacca
      301 gaggctacga aagaccccat ggcgaaggcg gcgccggccg gtgtcagtgg ttccgaggca
      361 acggagactg acaatgccat ctcaatccaa ggcgcttccc cactggacgt gggacgatct
      421 ggaggcggcg ctcaacgggt tcagccccct gggtccaaac gatccggtgc gggcgcatct
      481 gatgcgcgga ctgacggacg acgcgcaacg ggcgacgccg cgcgaactgc tgcagcaggt
      541 cctgagcgcg ggctgggtga tggcccaggt gggggcccag agcggcgcct cggctaccgc
      601 cccccgccgc gcagctgggt cgtccggctc ctgatcctgc tggtcctgat cgtcgtgacg
      661 gcggtgatga tgaaggcggt ggcggacctg gtcctcgcct atgcgggcca ggcccaggag
      721 atcgggcacg tcaccaatcc cgagctggtg caaagcggcc gcaaggattg acgggcgttc
      781 ggcctgtcgc atgaggcggc cggatctccg cgcccgaccg gcgatcagga gagaacagca
      841 ttcccccctt cgtgacggcc acctacgtcg ccttcttcgc cgccatgatc ttcggctggc
      901 tgaagggcgg ccatccggag cggctggcgg tagcggtgct gctgctgacc gagccggtga
      961 tctccaatcc gctcgtccac tggcggatcg acgacatcta tctcgattca acaatagaag
     1021 acgccttgag gtcgctcttc ctcggctggc tggctttcag atcggaccgc tggtggccgt
     1081 acgtcgcgac cgcggcgatg gcgctgaccg tgctggtcca cattcttacg gtcgtgaccg
     1141 acatttcgtg ggacgcagcc gtttcggcgc gggtcggact ggcgcttctg atgtacgtga
     1201 ccctgctggc cggagtggcc gagcgatgga tggcgggcga ggcgccggtc tgccggatcg
     1261 ggccgtgacc cggctctttc cgcacgcata ggcctgtggc gcctcatcta cgcgaccctc
     1321 ctcgccgggg tcggcgagcg ttggctggcc ggcgaacaac cggccggaga gggcccacaa
     1381 tggcgcaggc gcgtcggcgc tgcgtcttga acatataaac atatccttat atgtctgccg
     1441 gccttcccat cccgccggag ctccgccttg tcccgttcgt cgttccagtt cacgtccgaa
     1501 agcgtctccg agggccatcc cgacaaggtt gctgaccgca tctccgacac cgtcgtggac
     1561 ctgttcctgg gcaaggaccc ctacgcgcgg gtagcctgcg agaccctgac gacgacaaac
     1621 ctggtggtgc tggccggcga gatccgcggc cagggcatca tggacaccga cggaaactgg
     1681 gcgccgggcg tgcaggacga gatcgaggcc gcggtgcgcg ccgccgtgcg cgacatcggc
     1741 tatgagcagt cgggtttcca ctggaacaaa ttcgagttca tcaaccgcct gcacggccag
     1801 tcggccgaca tcgccgtggg cgtcgacgcc gccggcgaga aggacgaggg cgcgggcgat
     1861 cagggcatca tgttcggcta cgcctcgaac gagacccccg agctgatgcc ggccaccctg
     1921 cagtacagcc acaacatcct gaagcgcctc gccgaggtgc gccacggcgg cgacaagcgg
     1981 ctggagcccg acgccaagag ccaggtcacg atccagtacg aggacggcaa gccggtgcgc
     2041 gccacctcga tcgtcctgtc gacccagcac gccgcc
//
LOCUS       DASVVN010000538         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_520475,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000538 DASVVN010000000
VERSION     DASVVN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520475"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF686_08755"
     CDS             <1..360
                     /locus_tag="VF686_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase"
                     /protein_id="HEX8661945.1"
                     /translation="ALLVESGANDPGFNLRREPALIRHMQGQAKASFFSVLEPHGAYD
                     SRAETVTGSTSRIRDIVRLRGDGAEVIVLTLASGKTVALGLADDVAAGRRHTVTQDGQ
                     TYQWTGPWARFDRETRP"
     gene            357..>1523
                     /locus_tag="VF686_08760"
     CDS             357..>1523
                     /locus_tag="VF686_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661946.1"
                     /translation="MKSKLRWVIIGLIAVATVINYIDRNALAVMWPEIAPEIGATKED
                     YALLVTIFMVFYAIGQFAFGKLFDRIGVRMGFALSIGVWSISIALHALATSILSFSVF
                     RAMLGISEAGAWPGAVKANAEWFPPEERAFAQGIFIAGASIGAIISAPLIALLFLTLG
                     WKGTFVLVGALGFLWILPWLVIYRAGPDQYAMRAAAERTLEGPAAAQPVEAVVAPGYV
                     PTTTQMLGHRQSWGVILTRFLLDPIWWLFVSWLPIYLAETFGFDVKQIGIFAWVPYVG
                     AMIGSLFGGWLSGWLINSGRSVNLARKASITLGGVVMMPCLLIAPNITDPTIAVLVIA
                     GVLFGFQVAIGNIQTIPGDLFDGRSVGSLAGVGGMAAVAGTLITTWLVPAMTKVS"
BASE COUNT          221 a          529 c          493 g          280 t
ORIGIN      
        1 gccctgctgg tcgagagcgg ggccaacgat cccgggttca acctgcgccg cgagccggct
       61 ttgatccggc acatgcaggg tcaggcgaag gcgagcttct tctcggtgct ggaaccccat
      121 ggcgcctacg acagcagggc cgagaccgtc acgggctcga cgagccgcat ccgcgacatc
      181 gtccggctgc gaggcgacgg cgccgaggtg atcgtcctga ccctcgcctc cggcaagacg
      241 gtcgcgctgg gcctcgccga tgacgtcgcc gcgggccgcc gccacaccgt cacccaggac
      301 ggacaaacct atcagtggac ggggccctgg gcccggttcg accgggagac cagaccatga
      361 agtccaagct ccgttgggtc atcatcggcc tgatcgcggt cgcgaccgtg atcaactaca
      421 tcgaccgcaa cgccctggcg gtgatgtggc cagagatcgc gcccgagatc ggcgcgacca
      481 aggaagacta cgccctgctg gtgaccatct tcatggtctt ctacgcaatc ggccagttcg
      541 cgttcggcaa gctgttcgac aggatcggcg tgcggatggg cttcgccctg tcgatcgggg
      601 tctggtcgat ctccatcgcc ctgcacgccc tggccacctc catcctcagc ttcagcgtct
      661 tcagggccat gctgggcatc agcgaggccg gcgcctggcc cggagcggtc aaggccaacg
      721 ccgaatggtt tccaccggag gagcgggcct tcgctcaagg catcttcatt gccggggcct
      781 cgatcggcgc cattatctcc gcgccgctga tcgccttgct gttcctgacc ctgggctgga
      841 agggcacctt cgtgctcgtc ggggcgctgg gcttcctgtg gatcctgccc tggctggtca
      901 tctatcgcgc cggcccggat caatacgcga tgcgcgccgc ggcggagcga accctcgaag
      961 ggcctgcggc ggctcagccc gtcgaggccg tggtcgcacc cggctatgtc ccgaccacga
     1021 cccagatgct gggtcaccgt cagagctggg gcgtgatcct gacccggttc ctgctcgacc
     1081 cgatctggtg gctgttcgtt tcctggctgc cgatctatct ggccgagacc ttcggtttcg
     1141 acgtcaaaca gatcggcatc ttcgcctggg tgccctacgt cggcgccatg atcggcagcc
     1201 tgttcggcgg ctggctgtca ggctggctga tcaactccgg ccgctccgtg aatctggccc
     1261 gcaaggcctc catcaccctt ggcggcgtgg tcatgatgcc ctgcctgctg atcgcgccga
     1321 acatcaccga tccgaccatc gcggttctgg tcatcgccgg cgtcctgttc ggcttccagg
     1381 tcgccatcgg caacatccag accatccccg gggacctgtt cgacggccgc tcggtcggat
     1441 cgctggccgg cgtcggcggc atggccgcgg tggccgggac gctgatcacg acctggctgg
     1501 tgcccgccat gacgaaggtt tcg
//
LOCUS       DASVVN010000539         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_98786, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000539 DASVVN010000000
VERSION     DASVVN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98786"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..927
                     /locus_tag="VF686_08765"
     CDS             550..927
                     /locus_tag="VF686_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661947.1"
                     /translation="MAPRRDAPIFRLQQAAPPPEPLAEAPAAQQGQPAAPRPRQVAEV
                     TNTGERPPQQGARYYSVHRQNGRQPDTLAMPEPTYVDALAITMTQTPASQDLAEPEQG
                     PTLIRDGQGRTRPVPAASDGDHQ"
     gene            924..2030
                     /locus_tag="VF686_08770"
     CDS             924..2030
                     /locus_tag="VF686_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8661948.1"
                     /translation="MSQVAAPTSAPSRLPDLIALAEEGSSEKRRALLRELTDHFFGGS
                     ARTATEDTLYGAVLTRLTEDMEIAVRAELSARFATALDAPHNLIRRLANDEAAVAGAV
                     LASSPVLTDEDLLSVVRKHGQDHLRAVSARPSVSEAISDVIVERGDDVTLGTLLRNDG
                     ARLSRQASETAIERAKVNPDLHEAAVERASLPPDLLNEMYFVVEARLRTQILEQNARL
                     DPALLESAIAAGRTRIATDDGALPADYAECLAYVEELKAAGQLSPQMLARFLRSGGTT
                     SFLIALAQLSDIDFHTARQIIERRELDALAVVCKSADLDRALFLTYAVVLLNDDGDAM
                     AKAHSYARMYADLSRDAALRTLRFWRMRRGAQAA"
     gene            complement(2100..2534)
                     /locus_tag="VF686_08775"
     CDS             complement(2100..2534)
                     /locus_tag="VF686_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX8661949.1"
                     /translation="MEDNAQLLEMTADIVSAYVGNNNVQATEVPGLISSIHAALTQVS
                     NGAVEPEPEVKDPAVPVRKSITPDFLICLEDGRKFKSLKRHLRTKYNMSPEEYRAKWG
                     LPKDYPMVAPNYAKARSDLAKSMGLGQGGRKPARAAAGRAKK"
     gene            complement(2726..>3662)
                     /locus_tag="VF686_08780"
     CDS             complement(2726..>3662)
                     /locus_tag="VF686_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8661950.1"
                     /translation="IEQCASLSDADLVACARDTGPEHRFLMASRRSLSEVVTETLLSF
                     GESHVIEAVLRNNTARLCQTAVEGVVSLSRQEPQLCGHVLKRPELRPSGAYVMFWWSG
                     AEDRRTILQRFAVSREVMQEVAEDVFAMAAEENWQDPVSRKALQFIERRQRNRAAIAK
                     SPYGGLEQAVAAAATGGLNREVATEIAYLSGIKPITGAKILGDPGGEPLAILCKATGL
                     GKNDLLGLWRSMRRPETAEDGSLHPAWERVQVTYDMLAVDRAQTVLRYWNWSLSSALT
                     PALLRAIRDGEEEGIDDYSAPERAAMLALAENFGR"
BASE COUNT          569 a         1388 c         1124 g          581 t
ORIGIN      
        1 tgcgtccgga gccgtccctt gcgccgcgcc cacagtctcc cgatcgcgct tctcgccgcc
       61 acgacgctgg caggcgccgc catggcccag acgccccaac cggctgcccg gcaggacctg
      121 cggcagcttt cgtggcccgg aagaaacacg gccgcgccga cgcccaacgg gcctgccgcg
      181 acccgcccgg acctgcgccg ctccaatctt gtcatccctc atggcggttt cggcgcgagc
      241 ggatccgcac cggcagctcc cgccgcgacc gcgccccgtc ggaccctgac gcccgccaac
      301 gcctggctgc aaccggtctc gccgcccccc gcaccgccgc cggccccggc cccagccgcg
      361 gccatgcccg ctcccgtccg gacagaagcg ccctcgccgc ccccccgacc ggcctctcgc
      421 ccggtcgcga cccccgaata ccttcccgac caaggcgggc gcggccagcg cgctcccgcc
      481 gacatcgtct atgcggcgcc gccccaaccc gcggcttccg accccggccc ggatcagcca
      541 gccgatccga tggctccccg ccgcgacgcc ccgatcttcc gcctgcagca ggccgcgccc
      601 ccacccgaac ccctcgccga agcgcccgcc gcccagcagg gtcagccagc cgcgccccgg
      661 cctcgccaag tcgccgaggt gaccaacacc ggggagcgtc cgcctcaaca aggcgcccgc
      721 tactactcgg tccatcgcca gaatggccgt cagcccgata cgctggcgat gcccgagccg
      781 acctatgtcg acgccctggc catcaccatg acccagactc ccgcctcgca ggatctggct
      841 gagccggaac agggcccgac cttgatccgc gacggtcagg gccggacgcg tcccgtcccc
      901 gccgcttccg acggggacca tcagtgagcc aggttgcggc cccgacttcc gcgccctcgc
      961 gccttcccga cctgatcgcc ctcgccgaag agggctccag cgagaaacgg cgcgccctgc
     1021 tgcgggagct gaccgaccat ttcttcggcg gctcggcccg caccgcgacg gaagacaccc
     1081 tgtacggcgc cgttctgacc cggctgacgg aggacatgga gatcgccgtc cgcgccgagc
     1141 tttcggcccg tttcgccacg gctctcgacg cgccgcacaa cctgatccgc cggctggcca
     1201 atgacgaggc cgccgtcgcc ggcgctgtcc tggcctcctc gcccgtgctg accgacgagg
     1261 acctgctcag cgtcgtgcga aagcatggcc aggatcacct gcgtgccgtc tccgcccggc
     1321 catcggtgtc cgaggccatc tcggacgtca tcgtcgaacg cggcgacgac gtgaccctcg
     1381 gcacgctgct gcgcaacgac ggcgccagac tgtcccgtca ggcgtccgaa accgcgatcg
     1441 aacgggccaa ggtcaatccc gacctccacg aggcggcggt cgagcgcgcc agcctgccgc
     1501 ccgacctgct gaacgagatg tatttcgtgg tcgaggcccg gctgcgcacc cagattctgg
     1561 aacagaacgc gcgtctggat ccggccctgc tggaaagcgc catcgccgcc ggccgcaccc
     1621 gcatcgccac cgacgacggc gccctgcccg ccgactacgc cgaatgcctg gcctatgtcg
     1681 aagagctgaa ggccgctggc cagctgtcgc cccagatgct ggcccgcttc ctccgctcgg
     1741 gcggaacgac atccttcctg atcgccctgg cccagttgtc ggacatcgac ttccacaccg
     1801 cccgccagat catcgagcgc cgcgaactgg acgccttggc cgtggtctgc aagtcggcgg
     1861 acctggatcg ggccctgttc ctgacctatg cagtcgtgtt gctgaacgac gacggcgacg
     1921 ccatggccaa ggcgcactca tacgcccgca tgtacgccga cctcagccgc gacgccgccc
     1981 tgcgcaccct gcgcttctgg cgcatgcgcc gcggggctca ggcggcctga ccggcgggtc
     2041 ataaaaaacg cccgcctccg tttccggaag cgggcgcttc ataccatcga tcaggcggac
     2101 tatttcttgg cgcggccggc ggcggcgcgg gccggcttgc ggcccccctg gcccagaccc
     2161 atcgacttgg ccaggtccga acgggccttg gcgtagttcg gcgcgaccat cgggtagtct
     2221 ttgggcagac cccatttggc ccggtattct tcggggctca tattgtattt ggtgcgcagg
     2281 tggcgcttca gcgacttgaa cttgcggccg tcttccaggc agatcaggaa gtcgggcgtg
     2341 atcgacttgc gcaccggaac cgccgggtcc ttgacctcgg gctcgggttc gacggcgccg
     2401 ttggacacct gcgtcagggc tgcgtgaatg ctggagatca gccccggtac ttcagtcgcc
     2461 tgaacgttgt tgttaccgac gtaggcggac acgatgtccg cggtcatctc gagcagttga
     2521 gcgttgtctt ccacgtgcgg gccccgtctt gttgagatca attccggact gaatcagtca
     2581 gtgatgaggt caatagattt gttccgtatt gaaagcaact gtatcgccga tcatcggaac
     2641 gacgtctctc tgatcgcggg cggtttacta ctgcgttgcc agttcgggta gaaaatcccg
     2701 tttcgtgccg gttgcactgc ggtaatcagc gtccgaaatt ttccgccaga gccagcatgg
     2761 cggcgcgttc aggggcggag tagtcgtcga tcccctcttc ttccccgtcg cggatcgccc
     2821 tgagtagagc gggggtcagg gccgaagaca gggaccagtt ccagtaacgc agcaccgtct
     2881 gggcccggtc gaccgccagc atgtcatagg tgacctgcac ccgctcccac gccggatgaa
     2941 gcgacccgtc ctcggccgtt tccggccggc gcatcgaccg ccacaggccc aggaggtcgt
     3001 tcttgcccag acccgtggcc ttgcacagga tcgccagcgg ctccccaccg ggatcgccca
     3061 ggatcttggc cccggtgatc ggcttgatac ccgacagata ggcgatctcg gtcgccacct
     3121 cgcggttcag accgcccgtg gctgcggccg cgacggcctg ctccagcccg ccgtaggggc
     3181 tcttcgcgat agccgcgcga ttgcgctgcc ggcgctcgat gaattgcagc gccttgcgcg
     3241 acaccggatc ctgccaattc tcctcggcgg ccatggcgaa cacgtcctcg gcgacctcct
     3301 gcatgacttc acgcgagacg gcgaagcgct gcagaatagt ccggcgatcc tcggcccccg
     3361 accaccagaa catcacatag gccccggatg gccgaagctc aggccgcttg agcacatggc
     3421 cgcacagttg cggctcctgc cggctcaggc tgaccacgcc ctcgacagcg gtctggcaaa
     3481 ggcgggcggt gttgttgcgc agcaccgcct cgatcacatg gctttccccg aaactcagca
     3541 aggtctccgt gacgacctcg gacagcgaac ggcggctggc catcaggaac cggtgttcag
     3601 gaccggtgtc ccgcgcgcag gccaccagat cggcatcgga cagcgaggcg cattgttcga
     3661 tc
//
LOCUS       DASVVN010000540         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_232313,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000540 DASVVN010000000
VERSION     DASVVN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232313"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF686_08785"
     CDS             <1..582
                     /locus_tag="VF686_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8661951.1"
                     /translation="LTIARRMANIMDGDINVTSTVGEGSTFTFSVETDVVEWGRQAAA
                     EPIHAEVADGQALNVLVVEDHPVNRMILEAWLGSAGHASVSAENGQIAIEMAKDQTFD
                     LVIMDVNMPVMDGLTATRAIRAGEGANRETPIVVLSASARSEDHAAGLDAGADAYLNK
                     PIDFAALAELMNRVGSGRAGVRSCIENPQAAAA"
     gene            723..1226
                     /locus_tag="VF686_08790"
     CDS             723..1226
                     /locus_tag="VF686_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8661952.1"
                     /translation="MKKILTAAIAATLAMSSLGVASAAEAQSRGNYEQRQDRRDDRRD
                     DRGDRRDERRDDRNDRREWRQDNRDDRREARQDQRAYSQWQRSERRYSAGRYQAPRGY
                     QTRQWSYGQRMPSYYRSNQYVVNDYGRYGLRAPPRGYHYVRSGNDAVLTAVTTGLVVA
                     VIASLFN"
     gene            1312..>2282
                     /locus_tag="VF686_08795"
     CDS             1312..>2282
                     /locus_tag="VF686_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8661953.1"
                     /translation="MRAFAELLDRLSLTASRNAKVVLLRDYLKATPDPDRGWALAALT
                     GELTFDAAKPAMIRKAVEARVDAVLFGWSYDYVGDLAETVALIWPRDPDYRPNREPEL
                     GEVVEALRLAKRGEVKDLLEGWMDALEPKGRWALLKLMTGGLRVGLSARLAKTAAAMM
                     RPDAVSEPPDPDGAEAVTPLEPVDASDIEEIWHALEPPYADLFAWLEGRSERPSGDAP
                     GRFRPVMLAVAIDEAVDFPKLSPRDFAAEWKWDGIRVQAVRENGVARLYSRHGDEVTN
                     AFPDLMNALTFEGAIDGELLVMRDGQVAPFGDLQQRLNRKTVDAKLMA"
BASE COUNT          390 a          746 c          798 g          348 t
ORIGIN      
        1 ctgacgatcg cccgccgtat ggccaacatc atggacggcg acatcaacgt gacctcgaca
       61 gtaggggaag gctcgacctt caccttctcg gtcgagaccg acgtggtcga atggggacgc
      121 caggcggccg cggagccgat ccacgccgag gtggccgatg gtcaggcgct gaatgtgctg
      181 gtggtcgagg atcaccccgt caaccgcatg atccttgagg cctggctggg ttcggccggc
      241 cacgcctcgg tcagcgccga gaacggccag atcgccatcg aaatggccaa ggaccagacc
      301 ttcgatctgg tcatcatgga tgtgaacatg ccggtcatgg acggcttgac cgccacccgc
      361 gccatccgcg ccggagaggg cgccaaccgc gagacgccga tcgtcgtgct gtccgcctcg
      421 gcgcgcagcg aggatcacgc cgcgggtctg gacgccggcg ccgacgccta tctgaacaag
      481 ccgatcgact tcgccgccct cgccgagctg atgaaccgtg tcggctccgg ccgcgccggc
      541 gttcgcagct gtatcgagaa cccccaggcc gccgccgcct gaccgccgga catcgttaat
      601 caagtcctaa aggcgacctt ccgctgaatg gcggatgacc aacggcgttc acacctggct
      661 caagcgccgg ggaccagagt tcgggcaagt cgaaatcgtt cggccgcatc tggagacgtc
      721 tcatgaagaa gattctcacc gccgccatcg ccgcgacgct cgccatgagc tcgctgggtg
      781 tggcctccgc cgccgaagct caatcgcgcg gcaactacga acagcgccag gatcgccgcg
      841 acgaccgccg tgacgaccgg ggcgaccgcc gcgacgagcg ccgcgacgac cgcaatgatc
      901 gccgcgaatg gcgtcaggac aaccgcgacg accgccgcga agcccgtcag gaccagcgcg
      961 cgtacagcca gtggcaacgg tcggagcgcc gctacagcgc gggccgctac caggcgccgc
     1021 gcggctatca gacccgtcag tggtcgtatg gtcagcggat gccgtcttac taccgctcga
     1081 accagtatgt cgtgaacgac tacggccgtt acggcctgcg tgccccgccc cgtggatacc
     1141 actacgtccg cagcggcaat gacgcggtcc tgaccgccgt gacgaccggt ctggtggtcg
     1201 ctgtgattgc gagcctgttc aactaatcgg ccgcgcctga agagacgacg ccccggaggg
     1261 aaacctccgg ggcgtttttc gtttggagtc aggccgttta catgaggtcc gatgcgcgcc
     1321 ttcgccgaac tgctggaccg actgtcgctg acggcgtcgc gcaatgcgaa ggtggtgctg
     1381 ctgcgggact atctgaaggc gacgcccgat ccggaccgtg gctgggcgct ggcggccctg
     1441 accggcgagc tgaccttcga tgcggccaag ccggccatga tccgcaaggc ggtcgaggcg
     1501 cgggtcgatg cggtgctgtt cggctggtcc tacgactatg tcggcgatct ggccgagacg
     1561 gtggcgctca tctggccccg ggacccggac tacaggccca accgcgagcc cgaactgggc
     1621 gaggtggtcg aggccttgcg cctcgccaag aggggcgagg tcaaggattt gctggaaggc
     1681 tggatggacg cgctggagcc caaggggcgg tgggcgttgc tgaagctgat gacgggaggg
     1741 ctgcgcgttg gcctgtcggc gcgtctggcc aagacggcgg cggcgatgat gcgtccggac
     1801 gcggtcagcg aaccgccgga tcccgacggc gccgaggcgg tcaccccgct ggagcccgtc
     1861 gacgcgtcgg acatcgagga gatctggcac gcgctggagc cgccctacgc cgatctgttc
     1921 gcctggctgg agggtcggtc cgaaaggccc agcggcgacg cccccggccg gttccggccg
     1981 gtcatgctgg ccgtggcgat cgacgaggcg gtcgattttc cgaagctgtc accccgggat
     2041 ttcgcggccg aatggaagtg ggacggcatc cgcgtccagg ccgtgcgcga gaacggggtg
     2101 gcgcggctct attcccggca tggggacgag gtgaccaacg cctttcccga cctgatgaac
     2161 gccctgacct tcgaaggagc gatcgacggg gagttgctcg tgatgaggga tgggcaggtg
     2221 gcgccgttcg gggacctgca gcagaggctg aaccgcaaga ccgtcgacgc caagctgatg
     2281 gc
//
LOCUS       DASVVN010000541         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_20924, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000541 DASVVN010000000
VERSION     DASVVN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20924"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1208
                     /gene="plsX"
                     /locus_tag="VF686_08800"
     CDS             129..1208
                     /gene="plsX"
                     /locus_tag="VF686_08800"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8661954.1"
                     /translation="MSSPILISLDAMGGDHGPSVVVGGVRDYRKRHGGEGVRFLLHGD
                     ETAIRAEMAKCALTEDVCIVRHTDKTIAMDEKPAQAMRRGKGSSLWNAIEAVKAGEAG
                     AVVSAGNTGALMAISKLLLRMSAPGLERPAIVAGWPTPRGVTAVLDVGANVESDAAQL
                     VEFAIMGEAFMTAVHGIARPTIGILNVGSEDMKGHEEVREAARLLREGGFGLDYFGFV
                     EGTDIAKGTVDVVVTDGFTGNIALKTAEGVAKFIAGLMKEALSSSLTSKAGALLAMPA
                     LKRMSQKIDPNANNGGPLLGLNGIVVKSHGGATPGGYGAAIRVAVELARSDYLIKVSD
                     SFSRLTAVLEQPPAPPADPAMETSQ"
     gene            1205..2182
                     /locus_tag="VF686_08805"
     CDS             1205..2182
                     /locus_tag="VF686_08805"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX8661955.1"
                     /translation="MSVIRSAVTGVGSFLPEQVVTNADLAQIVDTSDEWIQERTGIRQ
                     RHKARDDQPTSDLAVEAAKKALADAGRTAAEVDMIIVATTTPDMTFPSVASIVQRKIG
                     APTCIAFDVQAVCSGFVYALSVADGFVARGLSKCAIVIGAEEMTRLMDWTDRSTCVLF
                     GDGAGAVVVEPREGQGTKEDKGLLGFALRCDGTKTDLLYVDGGPATTGAVGHLRMAGN
                     QVFRHAVVNIAEAITACCAAAKIEVADVDWFVPHQANQRIIKGVGDRLGLDEHKVIST
                     VATHANTSAASIPLALDAAIRDGRIKRGDLVLMEAMGGGLTWGACAFRL"
     gene            2258..2566
                     /locus_tag="VF686_08810"
     CDS             2258..2566
                     /locus_tag="VF686_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX8661956.1"
                     /translation="MAEHQTLTRADLCEAVHEEVGLSRQECSSLVERTLELIIESLER
                     GETVKLSGFGVFQVREKRARMGRNPKTGQPAAIHPRRVISFRASQIMKGRVHDAVVPA
                     "
BASE COUNT          452 a          851 c          910 g          403 t
ORIGIN      
        1 tgctgaagca actcaggccc accgacgaag gttgacgcag cggcggcgcg gttgcatccc
       61 cccgctgcgg cgctatcgtc gcctcactga caagcgccgc acggacggcg cgacggagtc
      121 gaaacggctt gtcatccccg atcctgattt cgctcgacgc catgggcgga gaccacggcc
      181 cctcggtcgt ggtcggcggc gtccgcgact accgcaaacg ccacggcggc gaaggcgtgc
      241 gcttcctgct gcacggcgac gagacggcga tccgggccga gatggccaag tgcgcgctga
      301 ccgaggacgt ctgcatcgtc cggcacaccg acaagacaat cgccatggac gaaaagccgg
      361 cgcaggccat gcgccggggc aagggctcca gcctgtggaa cgccatcgag gcggtcaagg
      421 cgggtgaggc cggggcggtc gtatcggcgg gcaacaccgg cgccctgatg gccatctcga
      481 agctgttgct gcgtatgtcg gcgcccggtc ttgagcgacc ggccatcgtg gccggctggc
      541 caacgcccag aggcgtcacg gccgtgctgg acgtcggcgc caatgtcgag agcgacgcgg
      601 cacagctggt cgagttcgcc atcatgggcg aggccttcat gaccgccgtt cacgggatcg
      661 ccagacctac catcggcatt ctgaacgtcg gctccgaaga catgaagggc catgaggagg
      721 tccgcgaggc ggcgcgcctg ctgcgtgagg gcggcttcgg tctggactat ttcgggttcg
      781 tcgagggcac ggacatcgcc aaggggacgg tggatgtggt ggtgaccgac ggcttcaccg
      841 gcaatatcgc gctgaagacg gccgaaggcg tggccaaatt catcgcgggg ctgatgaagg
      901 aggcgctgag ctcgagcctg acgtcaaagg ccggggcgct gctggccatg ccggcgctga
      961 agcgcatgag ccagaagatc gacccgaacg ccaacaacgg cgggcctctg ctggggctga
     1021 acgggatcgt ggtgaaaagc cacggcggcg cgacgccggg cggctatggc gccgccatcc
     1081 gcgtggctgt cgaactggcc cgcagcgact atctgatcaa ggtcagcgac agtttcagcc
     1141 gcctgaccgc cgtactcgaa cagccgcccg caccccccgc ggacccggcc atggagactt
     1201 cccagtgagc gtcattcgca gcgccgtgac cggcgtcggc tcgtttctgc cggaacaggt
     1261 cgtaaccaac gccgatctgg cgcagatcgt cgacacttcc gacgaatgga tccaggagcg
     1321 gaccggaatc cgtcagcggc acaaggcgcg cgacgatcag ccgaccagtg acctggccgt
     1381 cgaggcggcg aagaaggccc tcgccgacgc cggccggacg gcggccgagg tcgacatgat
     1441 catcgtcgcc acgaccacgc ccgacatgac cttcccgtcg gtcgcctcga tcgtgcagcg
     1501 caagatcggc gcgccgacct gtatcgcctt cgacgtacag gcggtgtgtt cgggcttcgt
     1561 ctacgccctg agcgtcgccg acggcttcgt cgcccggggg ttgtcgaaat gcgcgatcgt
     1621 catcggcgcc gaggagatga cccggctgat ggactggacc gaccggtcga cctgcgtcct
     1681 gttcggcgac ggggccgggg cggtcgtcgt cgagccgcgg gaggggcagg ggaccaagga
     1741 agacaagggg ctgctgggct tcgccctgcg ctgcgacggg accaagaccg atctgctcta
     1801 tgtcgacggc ggcccggcaa ccacgggggc ggtgggccat ctgcgcatgg cgggaaacca
     1861 ggtgttccgc cacgcggtgg tcaatatcgc cgaagccatc accgcctgtt gcgccgccgc
     1921 caaaatcgag gtcgcggacg tcgactggtt cgtgccgcat caggccaacc agcggatcat
     1981 caagggcgtc ggcgaccggc tcgggctgga cgagcacaag gtcatctcga ccgtcgccac
     2041 gcacgccaat acatcggcgg cctcgatccc gctggcgctc gacgccgcga tccgggacgg
     2101 gcgtatcaag cgcggcgatc tggttctgat ggaggccatg ggcggcggtc tgacatgggg
     2161 cgcctgcgcg tttcgtctgt agggcgtttt tccggctttc catttgacga cgcttgccct
     2221 tttatgtcgc caaccgacct ttggagggga gcggaccttg gccgaacacc agaccctgac
     2281 gcgcgcggac ctgtgtgaag cggtgcacga ggaagtcgga ctttcccggc aggaatgctc
     2341 ctcgctggtc gaacgcacgc tggaactgat catcgagtcg ctcgagcgcg gggaaacggt
     2401 caagctttcg ggcttcggcg tgttccaggt acgcgaaaag cgcgcccgca tgggccgtaa
     2461 tccgaagacg ggccagccgg cggcgatcca tccgcgccgg gtcatcagtt tccgggcctc
     2521 gcagatcatg aagggccggg ttcacgacgc tgtcgtgccc gcctgacgcc cgtggccaag
     2581 agccccaacg ccttccggac gatttcagaa gcctct
//
LOCUS       DASVVN010000542         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_179926,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000542 DASVVN010000000
VERSION     DASVVN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179926"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..877)
                     /locus_tag="VF686_08815"
     CDS             complement(41..877)
                     /locus_tag="VF686_08815"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8661957.1"
                     /translation="MTQTLAEALTLTDDGAGGWTARLDGGFSNAPQSAPQERGAPFGG
                     LMAALAAGAMRQGLGIETPLQTLTIQYLAAARFEDAVFTPAMNRGGRNVAYASVTGGQ
                     GDRPAIQALGTYGRDVAGPVLTLLSASPRPVEELEPTPMNPVFGPWFTRHIDHRFVEG
                     PKLFGENAGKTPELGCWMRTIDAAPLDEARLLFLLDGLYPTYFTAFPAPPIISASVDL
                     RADLLTELTPDTSPEGWAYFHFTSRDVGSGWAVEDGVAWAPDGRPLALVRQRRKLMPT
                     RA"
     gene            1047..>1512
                     /locus_tag="VF686_08820"
     CDS             1047..>1512
                     /locus_tag="VF686_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8661958.1"
                     /translation="MGRTADYSRQSCSIAATLEVIGDPWTLLVIRDAFNGVSRFEQWQ
                     ERLGVARNVLAARLKTLVQHGVLEPHLYSERPPRNEYRLTAKGKDLYGVLVTLHAWGA
                     RHVYGEADSGFAMIHKDCGQNLAPRIACGHCNEIVKPRDIQLVHAKDCPTVGD"
BASE COUNT          256 a          514 c          525 g          217 t
ORIGIN      
        1 cggttggcgc cccgaattcg caaaaccgtg ccacgggctc ttacgcgcgg gtcggcatca
       61 gcttccggcg ctggcgaacc agcgccagag gcctcccgtc aggcgcccag gcgaccccgt
      121 cctcgaccgc ccagccgctg ccgacgtcgc ggctggtgaa gtggaaatag gcccagccct
      181 ccggcgaagt gtccggcgtc aattcggtca gcaggtcggc gcgcaaatca acgctggcgg
      241 agatgatcgg cggcgccggg aaggctgtga aataggtcgg gtacaggccg tccagcagga
      301 acagcagccg cgcctcgtcc agcggagcgg cgtcgatggt ccgcatccag cagccgagtt
      361 ccggagtctt gccggcgttc tcaccgaaca gcttcggacc ctccacgaag cgatggtcga
      421 tgtggcgggt gaaccagggt ccgaagacgg ggttcatcgg cgtcggctca agctcctcga
      481 cgggccgggg tgaagcgctc agcagcgtca ggacaggccc ggcgacatcg cggccgtatg
      541 tgcccagcgc ctgaatcgcc gggcggtcgc cctgaccgcc ggtgaccgac gcataggcga
      601 cgttgcggcc gccgcgattc atcgcgggcg tgaagaccgc gtcctcgaac cgggcggcgg
      661 cgagatactg gatggtcagg gtctggagcg gcgtctcgat ccccagcccc tgacgcatgg
      721 cgcccgcggc gagcgccgcc atcagcccgc cgaagggggc gccgcgttcc tgcggggccg
      781 actgcggagc attggaaaag ccgccgtcca gccgcgccgt ccagcccccc gcgccgtcgt
      841 cggtcagggt cagggcttcg gccagggtct gggtcatgcg cgttgctatc gtggggaaag
      901 ggcgggcgga agacagggga ggccttccgc ccgaagttcg ggatcaggaa acgccgcagc
      961 gcctccgtag ccaaacaaga ctgacctagt ccgtccagat tggcaaccct cttgccaaaa
     1021 ggctgacgcg gggtcacttt cccaccatgg gacgcaccgc agactactca cgccagagct
     1081 gttcgatcgc cgcgacgctg gaggtcatcg gcgatccgtg gacgctgctg gtcatccgcg
     1141 acgccttcaa cggggtcagc cggttcgaac agtggcagga gcggctgggc gtggcccgca
     1201 acgttctggc cgctcgcctg aagaccctcg tccagcacgg ggtgctggaa ccgcatctgt
     1261 attccgaacg tccgccccgc aacgagtacc ggctgacggc caagggcaag gacctgtacg
     1321 gggtgctggt caccctgcac gcctggggcg cgcggcacgt ctatggcgag gcggattccg
     1381 gcttcgccat gatccacaag gactgcggcc agaacctcgc gccgcgcatc gcctgcggcc
     1441 actgcaacga gatcgtcaag ccgcgcgaca tccagctggt ccacgccaag gactgcccga
     1501 cggtcgggga cg
//
LOCUS       DASVVN010000543         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_467568,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000543 DASVVN010000000
VERSION     DASVVN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467568"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2198
                     /locus_tag="VF686_08825"
     CDS             <1..>2198
                     /locus_tag="VF686_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8661959.1"
                     /translation="AAAFFFLPVRELPDVDPPIVSVSTSYAGASAEVIESRITEPIEQ
                     QIAGIQGVERINSSSRDGRSNVSIEFSLDRNIDDAANDVRDRVSRVVGRLPDQAQPPE
                     VSKADTDSQPIMILFLRSTAMNRLELTDYADRYLIDRLATVPGVAQVQIYGEQRYAMR
                     IWLDAAALAARGLTVTDVETALTAQNVELPAGQLESGQKDYTVRVARTYARAEDFRQL
                     PIGTRGSSGVAATTAGAASSATALPSGATGAIQGQSGYVTRLGDIARIEEAPAEDRRL
                     FRGNGQDQIGLAVTRQAQSNDLAISKGVEQMLEQVRPSLPEGTELVVGSDNSVFTSHA
                     IEEVWITIGISLALVALVNFIFLGSIRAAFIPSIVAPICLLSTFIVLAPLGFSLNLLT
                     LLALVLAIGLVVDDAIVVTENIQRRLDHGEPPLVAAERGTRQVFFAVVATTIVLLAVF
                     APLLFLPGYVGRLFVELAAAIAAAVAFSAFLALTLSPMLASRILKPASGGGWLARRVD
                     RAMEALRNSYQNSLEALLGRRIAVVGVSVLVLAVGGGAAAMFFALPNELVPAEDRGRV
                     QIRVQGPEGAGFDYTRKIMMGIEPILAEYKASGEADSYLISAPGFGGGSFNSGNGSLI
                     LSDWSERDRAADEIAQELNGKLRGQTDAQVNASVPGAFQRGGGNSNSIEMVVTGAEYE
                     DIYNWLQPVLAASLENPGLSRPRLNYEPNAPRLLVDVDPEKAAALGVSSQA"
BASE COUNT          317 a          838 c          691 g          352 t
ORIGIN      
        1 gccgcggcct tcttcttcct gccggtgcgc gaactgcccg acgtggaccc gcccatcgtc
       61 tcggtcagca ccagctacgc cggcgcctcg gccgaggtca tagaaagccg gatcaccgag
      121 ccgatcgaac agcagatcgc cggcatccag ggcgttgagc ggatcaattc gtccagccgc
      181 gacggccgct cgaacgtctc gatcgaattc tcgctggacc gcaatatcga cgacgccgcc
      241 aacgatgtgc gcgaccgcgt ctcccgcgtc gtcggccgcc tgcccgatca ggcccagccg
      301 cccgaagtgt ccaaggccga caccgacagc cagccgatca tgatcctgtt cctgcgctcc
      361 acggccatga accggctgga gctgacggac tacgccgacc gctatctgat cgaccgtctg
      421 gccaccgtgc ccggcgtcgc ccaggtgcag atctacggcg agcagcgcta cgccatgcgc
      481 atctggctgg atgccgccgc cctcgcggcg cgcggcctga cggtcaccga tgtcgaaacc
      541 gccctgaccg cccagaacgt cgaactgccc gctggccagc tggagtcggg ccagaaggac
      601 tataccgtcc gcgtcgcccg cacctacgcc cgcgccgagg acttccgcca gctgcccatc
      661 ggcacgcgcg gctccagcgg cgtggccgcg acgaccgcgg gcgcggcgtc cagcgccacg
      721 gccctgccca gcggcgcgac cggcgccatt cagggccagt ccggctacgt cacccgtctc
      781 ggcgacatcg cccgcatcga ggaggcgccg gccgaggacc gccgcctgtt ccgcggcaac
      841 ggacaggacc agatcggcct cgccgtcacc cgtcaggccc agtccaacga cctcgccatc
      901 tccaagggcg tcgagcagat gctggagcag gtccgcccca gcctgcctga gggcaccgaa
      961 ctggtcgtcg gctcggacaa ctccgtcttc acctcccacg ccatcgagga ggtctggatc
     1021 accatcggca tctcgctggc gctggtggcg ctggtgaact tcatcttcct gggcagcatc
     1081 cgcgcggcgt tcatcccctc gatcgttgcg cccatctgcc tgctctcgac cttcatcgtg
     1141 cttgcgccgc tgggcttctc cctgaacctg ctcaccctgc tggcgctggt tctggccatc
     1201 ggtctggtgg tcgacgacgc tatcgtggtg accgagaaca tccagcgccg cctcgaccac
     1261 ggcgagcctc cgctggtggc cgccgagcgc ggcacccgtc aggtcttctt cgccgtcgtg
     1321 gctacgacca tcgtcctgct ggccgtgttc gcgcccctgc tgttcctgcc cggctatgtc
     1381 ggccgtctgt tcgtcgaact ggcggcggcg atcgcggcgg ccgtggcctt ctccgccttc
     1441 ctggccctga ccctgtcgcc catgctggcc tcgcgcatcc tcaagcccgc ctcgggcggc
     1501 ggctggctgg cccgccgggt cgaccgcgcc atggaggcgc tgcgcaactc gtaccagaac
     1561 tcgcttgagg ccctgctggg ccggcggatc gccgtcgtcg gcgtgtccgt tctcgtgctc
     1621 gccgtcggcg ggggcgcggc ggcgatgttc ttcgcactgc ccaacgaact ggttccggcc
     1681 gaggatcgcg gccgggtgca gatccgggtt cagggccccg agggcgccgg cttcgactac
     1741 acccgcaaga tcatgatggg gatcgaaccc atcctggccg agtacaaggc cagcggggag
     1801 gccgattcct atctgatctc cgcccccggc ttcggcggcg gcagcttcaa ctccggcaac
     1861 ggcagcctga tcctcagcga ctggtccgaa cgcgaccgcg ccgccgacga gatcgcccag
     1921 gaactgaacg gcaagctgcg cggccagacc gacgcccagg tcaacgcttc ggtccccggc
     1981 gccttccagc gcggcggggg caactccaac tcgatcgaga tggtcgtgac cggagccgaa
     2041 tacgaggaca tctacaactg gcttcagccc gtgctggccg cctcgctcga gaaccccggc
     2101 ctgtcgcgcc cgcgcctgaa ctatgagccg aacgctccgc gtctgctggt cgacgtcgat
     2161 ccggagaagg ccgccgcgct cggcgtctcg tcccaggc
//
LOCUS       DASVVN010000544         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_494630,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000544 DASVVN010000000
VERSION     DASVVN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494630"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VF686_08830"
     CDS             complement(<1..821)
                     /locus_tag="VF686_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8661960.1"
                     /translation="MRHVPGVIILTALLAMAGQSAAQTPTAAPTPAPPPPSLKAANSA
                     PAADPAFACHVGLYRTDDGRLVDIAPVTGPGGRWRTLDGQTGRLTVEADGSWKNTRGW
                     TDRVEGSQVQLGACDDRLTFDGREAVRVDQTAVETTFAGAGGVMLKGRLILPPGGGPA
                     PIMVEVHGSEATSALDFNWFQRLSPASGVGVFVYDKRGTGGSQGTYTQDFDLLAGDAA
                     AAAVEARRLAGARAGRVGLHGGSQGGWVAPLAATLTPVDFVIVGFGMAEGPLAEDR"
     gene            1056..1835
                     /locus_tag="VF686_08835"
     CDS             1056..1835
                     /locus_tag="VF686_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8661961.1"
                     /translation="METFLLFFVVGVLAQSVDGALGMAYGVISSSVLLAFGVPPAMAS
                     ASVHGAEVFTTAASAGSHVAHRNVDWRLLVPLAIAGVIGGCLGAYVLAGIEGDVIKPF
                     VIAYLALMGFYILWRAGHDILIRHIPSWVTWPLGVVGGFLDAVGGGGWGPTVSSTLVG
                     AGQEPRRAIGTTNTAEFFLTVAISATFVWALMTGHWAEAGALEHHAAAIGGLVAGGLV
                     AAPFAGLITKRVPRKAMTYAVGALLLLLAGFQGLQLAGVLA"
BASE COUNT          247 a          621 c          718 g          292 t
ORIGIN      
        1 cggtcctcgg ccaagggacc ctcggccatg ccaaagccga cgatgacgaa gtcgaccggc
       61 gtcagggtgg cggcgagagg cgcgacccag ccgccctgac tgccgccatg caggccgacg
      121 cggccggcgc gggcgccggc gagacggcgc gcctcgaccg cggcggcggc ggcgtcgccg
      181 gccagcagat cgaagtcctg ggtgtaggtc ccctgcgaac cgcccgtgcc gcgcttgtca
      241 tagacgaaga cgccgacgcc cgaggcgggc gacagccgct ggaaccagtt gaagtccagg
      301 gcgctggtcg cttccgatcc gtgcacctcg accatgatcg gggcggggcc gccgccgggc
      361 gggaggatca gccgaccctt gagcatcacg ccgccggcgc cggcgaaggt ggtctcgacc
      421 gcggtctggt cgacgcgcac cgcctcgcgt ccgtcgaagg tcaggcggtc gtcgcaggcg
      481 ccgagctgga cctgcgagcc ctcgacccgg tcggtccagc cgcgggtgtt cttccatgag
      541 ccgtcggctt cgacggtcag gcggccggtc tggccgtcga gggtccgcca gcgtccgccg
      601 gggcccgtca cgggcgcgat atccaccagc cggccgtcgt cggtccggta caggccgacg
      661 tggcaggcga aggccggatc ggcggctggc gcggagttgg ccgccttcag ggagggcggc
      721 ggcggggccg gagtcggggc cgcggtcggg gtctgggccg ccgactgccc ggccatggcc
      781 agcagcgccg tcaggatgat cacaccggga acgtgtcgca tgcgggactc cttctgtcgg
      841 agcccgggct agagacgctc cgcgcggggg tccagttacg attttgacag gcaggcgacg
      901 ggaaatgcgc cgatggcggg atcgtgaccc cgcctcacaa gcccgtcagg gtggttcctt
      961 ccgggcaggc ctgcgcccaa gctgggccgg cgaggtgcaa ccaaagacag gacggagctt
     1021 tgcgctcaac aggcttcact gttcgatcgg gctgaatgga aaccttcctg ctgttcttcg
     1081 tggtcggcgt gctggcgcag tcggtcgatg gcgccctcgg catggcctat ggggtgatct
     1141 cgtcgtcggt gctgctggcg ttcggcgttc cgccggccat ggcctcggcc agcgtgcatg
     1201 gagcagaggt cttcaccacc gcggcctcgg cgggcagcca tgtggcgcac aggaatgtgg
     1261 actggcggct gctggtcccg ctggccatcg ccggcgtgat cggggggtgt ctcggcgcct
     1321 atgtgctggc gggcatcgaa ggcgacgtga tcaagccgtt cgtcatcgcc tatctggcgc
     1381 tgatgggttt ctacatcctg tggcgggccg gccacgacat cctgatccgt cacatcccgt
     1441 cgtgggtgac ctggccgctg ggcgtggtcg gaggctttct cgacgccgtg ggcggaggcg
     1501 gctgggggcc gacggtgtcc tcgaccctgg tcggggcggg acaggagccg cgccgcgcca
     1561 tcggcacgac caatacggcc gaattctttc tgaccgtggc gatcagcgcc accttcgtct
     1621 gggccttgat gaccggccac tgggccgagg ccggggcgct ggagcatcac gcggcggcga
     1681 tcggcgggct ggtcgccggc gggctggtgg cggcgccgtt cgcgggtctg atcaccaaac
     1741 gggtgccccg caaggcgatg acctatgcgg tgggagccct gctgctgctg ctggccgggt
     1801 tccagggact gcagctggcg ggggtgctgg cctgatacga aaagggcgcg accggcctgc
     1861 gccgatccca ccccttcg
//
LOCUS       DASVVN010000545         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_126069,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000545 DASVVN010000000
VERSION     DASVVN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126069"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF686_08840"
     CDS             complement(<1..382)
                     /locus_tag="VF686_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8661962.1"
                     /translation="MKTVSAVALDDTDRKMIRALQTDGRMTNADLARTVNLSESACLR
                     RLRALEAEGVISRYAAIINERAVGLPISVFVTVTLSSQAEASLTAFETAIATVPEVVE
                     CYLMTGGSDYLLRLVVRDVDDLERV"
     gene            526..1644
                     /gene="ald"
                     /locus_tag="VF686_08845"
     CDS             526..1644
                     /gene="ald"
                     /locus_tag="VF686_08845"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX8661963.1"
                     /translation="MRVGVPKEIKKNEHRIGLTPTAVREYVAHGHTVSIETGAGLGAG
                     FTDADYKKAGAAIVADAKTVFADNDMIVKVKEPQKVEWEMLREGQILFTYLHLAPDPE
                     QTKGLLASKCAAVAYETVTNAQGGLPLLAPMSEVAGRIAVFSAAETLLKHNGGMGLLF
                     CGVPGVAPARVLVLGGGVVGLNAARMAMGLGAEVVVLERSIPRMAYIDEVTQGRVITR
                     YSAIGAIEEELVKADVIIGAVLVPGAEAPKLIKREHLKQMKPGSVLVDVAIDQGGCFE
                     TSHATTHEDPTYVIDGVVHYCVANMPGAAPRTSSEALNNATLPFGLALANKGLDALKS
                     NPHLARGLNVLNGELTYPAVAEALDMPWSDPFGVWADA"
     gene            2186..3244
                     /gene="obgE"
                     /locus_tag="VF686_08850"
     CDS             2186..3244
                     /gene="obgE"
                     /locus_tag="VF686_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX8661964.1"
                     /translation="MKFLDQAKIYIRSGNGGAGAVSFRREKFIPNGGPDGGDGGNGGN
                     VWITAVEGLNTLIDYRYQQHFKAQTGVHGMGRQMHGAKGEDVILKVPVGTQVLDDDKE
                     TVLLDMDVAGKTELLLKGGNGGWGNVHFKGPQNQAPAYANPGQEGQERWIWLRLKLIA
                     DVGLAGLPNAGKSTFLAAASAARPKIADYPFTTLVPNLGVVDLSATERFVIADIPGLI
                     EGASEGAGLGTRFLGHVERSGCLIHLIDGTQDDVAGAYHTIRHELEAYGAGLADKAEV
                     LALNKIDALTPEAREEKAAELEAAAGRRPMLVSGVSGEGVTELLRAAWTEVRKDRGEI
                     APAEEDETVMETPGGWAP"
BASE COUNT          643 a         1205 c         1272 g          550 t
ORIGIN      
        1 ggacgcgttc cagatcgtcg acgtcccgca ccaccagccg caggagatag tccgagccgc
       61 cggtcatcag atagcattcg accacctcgg gcacggtggc gatggcggtt tcgaacgccg
      121 tcagggaggc ctcggcctgc gacgacaggg tcacggtcac gaagacgctg atcggcaggc
      181 cgacggcgcg ttcgttgatg atggcggcgt agcggctgat gaccccctcg gcctccagcg
      241 cgcgcaggcg gcgcaggcag gcgctctcgg acaggttcac cgtccgggcc agatcggcgt
      301 tggtcatgcg gccgtcggtc tgcagggcgc ggatcatctt ccggtcggtg tcgtcaaggg
      361 cgacggcgga aacagtcttc atttggcgtc tcgttcgcag attctgcgag tgtatggcgt
      421 tcagtcgccg aaaagaacag gaacctgcgg ccggaccgcc gctagattgc cgcgcgaaac
      481 gaggcggccg gagaaccgcc tgtgtccatc acagaggaaa cggtcatgcg cgtcggcgtc
      541 cccaaggaaa tcaagaagaa cgaacaccgc atcggcctga cgccgacggc ggtgcgcgaa
      601 tacgtcgccc acggccatac ggtctcgatc gagaccggcg ccggtctggg cgcaggcttc
      661 accgacgcgg actacaagaa ggcgggcgcc gccattgtcg ccgatgcgaa gaccgtgttc
      721 gccgacaacg acatgatcgt gaaggtgaag gagccgcaga aggtcgagtg ggaaatgctc
      781 cgcgagggcc agatcctgtt cacctatctg cacctggcgc ccgatcccga gcagaccaag
      841 ggcctgctgg cctcgaaatg cgcggctgtg gcctatgaaa ccgtgaccaa tgcgcagggc
      901 ggcctgccgc tgctggcgcc gatgtcggaa gtggccggcc ggatcgccgt cttctcggcc
      961 gccgagaccc tgctgaagca caacggcggc atgggcctgc tgttctgcgg cgttccgggc
     1021 gtggctccgg cccgcgtgct ggtgctgggc ggcggcgtcg tcggcctgaa cgcggcccgc
     1081 atggccatgg gtctgggggc cgaggtcgtg gtgctggaac gctcgatccc gcgcatggcc
     1141 tatatcgacg aggtcaccca gggccgcgtc atcacccgct attcggccat cggcgcgatc
     1201 gaggaagaac tggtcaaggc cgacgtcatc atcggcgccg ttctggtgcc gggggccgaa
     1261 gctccgaagc tgatcaagcg cgagcacctg aagcagatga agcccggctc ggtgctggtc
     1321 gacgtcgcca tcgaccaggg cggctgtttc gagaccagcc acgccacgac ccacgaagat
     1381 ccgacctatg tcatcgacgg cgtcgtccat tactgcgtcg ccaacatgcc gggcgccgcg
     1441 ccgcgcacct cgtcggaagc cctgaacaac gccaccctgc cgttcggtct ggcgctggcc
     1501 aacaagggtc tggacgcgct gaagtccaac ccgcatctgg cgcgcggcct gaacgtgctg
     1561 aacggcgaac tgacctatcc ggccgtcgcc gaggcgctgg acatgccgtg gagcgacccg
     1621 ttcggggtgt gggccgacgc ctgatttcag acggacgccg aatgctgggc cccggtggag
     1681 acgccggggc ctttttcttg gccggcgggt gtgcggaaaa agacagtgaa gcgtgcacag
     1741 agtgcactgt gtgcggaaga aaaacacagt ctactgcgtc tacagcgtct actcagtcca
     1801 ctccgttcgc atggccggcc gtcgggattc gattgtcaaa gatcgtggag agcgtttcag
     1861 acccggattg tcgcacgggc tgttcctgcg gctacggaat gacgctgctt tttcccgata
     1921 gatcaatgga atcagcgggt tggtcgggcg aagtcgtctg gctggcggcg gattgccagc
     1981 atcgtctcct ccctgttcgc gcagcgaatg gggaggggga ccgcgaagcg gtggaggggc
     2041 gccggaccgc gctccctgct tctacccctc cacccccgcg ccaagcggcg cggcggtccc
     2101 cctccccatc gcctatggcg acggggagga gacggcgcgc gagccccgcc tgtaaatcgc
     2161 cgctctcttg cggcataagc ccgcgatgaa gtttctcgac caggccaaga tctatatccg
     2221 ctccggcaac ggcggcgcgg gcgccgtctc gttccggcgc gaaaaattca ttccgaacgg
     2281 cggaccggac ggcggcgacg gcggcaatgg cggcaacgtg tggatcacgg ccgtcgaggg
     2341 cctgaacacc ctgatcgact accgctacca gcagcatttc aaggcgcaga cgggcgtcca
     2401 cgggatgggc cggcagatgc acggcgccaa gggcgaggac gtcatcctga aggtgccggt
     2461 cgggacgcag gtgctggacg acgacaagga gacggtgctg ctggacatgg acgtggccgg
     2521 caagaccgaa ctgctgctca agggcggtaa cggcggctgg ggcaacgtcc atttcaaggg
     2581 gccgcagaac caggcgccgg cctacgccaa ccccggtcag gaaggccagg aacgctggat
     2641 ctggctgcgg ctgaagctga tcgccgacgt cggtctggcc ggcctgccca atgcgggcaa
     2701 gtcgaccttc ctcgcagccg ccagcgcggc gcggcccaag atcgccgact atccgttcac
     2761 gaccttggtg cccaacctcg gcgtggtcga cctgtcggcc accgaacggt tcgtgatcgc
     2821 cgacatcccc ggcctgatcg aaggggcctc ggaaggggcc ggtctgggca cccgcttcct
     2881 cggccacgtc gagcgctcgg gctgtctgat ccatctgatc gacgggacac aggacgacgt
     2941 cgccggcgcg tatcacacca tccgccacga gctggaggct tacggggcgg gtctggccga
     3001 caaggccgag gtgctggccc tgaacaagat cgacgccctg acccccgagg cgcgcgagga
     3061 gaaggccgcc gagctggagg ccgccgccgg ccgccggccc atgctggtgt ccggcgtctc
     3121 gggcgagggc gtgaccgaac ttctgcgcgc cgcctggacc gaggtccgca aggaccgcgg
     3181 cgagatcgcc ccggccgaag aggacgaaac cgtgatggaa acgcccggcg gctgggcgcc
     3241 ttagggctgc actgccttgt catcccggac ggcgcgacgc gctgatccgg gacgccgagc
     3301 ccgacggaga actctcccgg aagctgcgtg agcagctgtc cgggagccgg gtcgacacag
     3361 gcgcggcact cacctgtctc ccggacaatc gcgccgcgat ttccgggagc gaccatggcg
     3421 gttgatcgcc gtcccggatc gccgcctttg gcggcgtccg ggatgacaaa ggaggaacct
     3481 gggccgcctc ccgtcgcttc cttggcgaag gaggccgtca tgaacgacaa acccatccgg
     3541 accgaaggcc gcgacgacaa cggcgccatt ccgctgggcc gccccgatgc gtcgacgtcc
     3601 gatgcggcgg tgaaacgcaa tgcggagcgc gaacagcaga cggccgggcc cgacggcccc
     3661 gacgccacgg
//
LOCUS       DASVVN010000546         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_180078,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000546 DASVVN010000000
VERSION     DASVVN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180078"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..477
                     /locus_tag="VF686_08855"
     CDS             265..477
                     /locus_tag="VF686_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8661965.1"
                     /translation="MATGTVKWYNSTKGYGFIAPDDGGKDVFVHASAVEVSDLGTLNE
                     NQKISYEVERDSRSGKESAGRLKAAE"
     gene            578..850
                     /locus_tag="VF686_08860"
     CDS             578..850
                     /locus_tag="VF686_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661966.1"
                     /translation="MTPLADMIPTMSDPDLKALRLNALRLSASTTASLTQIASATDVI
                     PLIDEEIARRAADKTATAVKKPRAPAKKKVVPASGHQTALPDSTAA"
     gene            complement(847..2016)
                     /locus_tag="VF686_08865"
     CDS             complement(847..2016)
                     /locus_tag="VF686_08865"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX8661967.1"
                     /translation="MTKLKNSQGFSTRAIHAGQRPDPTTGAVMTPIYATSTYAQESPG
                     VNKGYEYARGKNPTREAFEACLADLEGGTHGFGFASGMAATSTALELLDAGSHIVTGD
                     DLYGGSWRLFERVRRRSMGLDFAYVDLSDLAKVEAAITPKTKLIWAETPTNPLMKLAD
                     IEGLSRIAKAHGLLLIVDNTFATPWSQQPLKLGADVVMHSATKYLNGHSDIIGGALVT
                     ANPDLAKEIKFLQNSVGGVMGPFDAFLANRGLKTLGLRMKAHNENALAVARWLEDRKS
                     GVARVIYPGLISHPQHELASRQMNGQYGGMVTVLIDGDLERTKRVLERVQVFTLAESL
                     GGVESLVNHPAIMTHASVPKEVREAGGVTDNLIRLSVGVEEVEDLIADLDQALGA"
     gene            complement(2016..>2254)
                     /locus_tag="VF686_08870"
     CDS             complement(2016..>2254)
                     /locus_tag="VF686_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661968.1"
                     /translation="RDLSFLLLHARRDEAVPVRATLELAEDLRAAGAAVEVQGLDFDG
                     HQYDLSTWCAVMSRTRAFMESRHPGNIGRPDQDN"
BASE COUNT          379 a          800 c          687 g          388 t
ORIGIN      
        1 gcgtgatcca cgtgacggac accgtctcga tgccgaactg acgaaacccc ggcggagcct
       61 gcatttgcgg gctccgccgg tcgcctctcg aatcacgacc cttgctattg cgccggaaag
      121 cgcccacttg aggcatcgtc gatctcttgc gaaacgctgc tgcccctttg catcgcgcac
      181 cgaggtccaa agccgatccc atccagaccc gacaggccga cccggaacct cttccggcgg
      241 tcaacgccta acggaggtct caaaatggct accggcactg ttaaatggta caactccacc
      301 aagggctacg gcttcatcgc tcccgatgat ggcggcaagg acgtcttcgt ccacgcctcg
      361 gccgttgagg tctcggacct cggcacgctc aacgaaaacc agaagatctc ctacgaagtc
      421 gaacgcgact cgcgctcggg caaggaatct gccggtcgcc tcaaggctgc tgaatagttt
      481 tcaagcgacc ctgccggagc tgatagctcc ggctgatgga agggccggct gccgaacagg
      541 cgccggcccg actgtttttc aacctctgga gacgccgatg accccgcttg ccgacatgat
      601 ccccacgatg agcgatccgg acctgaaggc cctgcgcctg aacgccctgc gcctgtccgc
      661 ctcgaccacg gcctcgctga cgcagatcgc ctccgccacc gacgtcattc cgctgatcga
      721 cgaagagatc gcccgccgcg ccgctgacaa gacggccacg gccgtcaaga agccccgcgc
      781 cccggccaag aagaaggtcg tccccgccag cggccaccag accgccctgc cggacagcac
      841 ggcagcctag gcccccagcg cctgatccag atccgcgatc aggtcctcga cctcttccac
      901 gccgaccgac agccggatca ggttgtcggt cacgccgccc gcctcgcgca cctctttcgg
      961 cacgctggcg tgggtcatga tggccgggtg gttgaccaga ctttccacgc cgcccagcga
     1021 ctccgccagg gtgaacacct gcacccgttc cagcacccgt ttcgtccgct ccagatcgcc
     1081 gtcgatcagg accgtgacca tgccgccgta ctggccgttc atctggcgtg aggccagttc
     1141 gtgctgcgga tggctgatca ggccggggta gatgaccctc gccacccccg acttccggtc
     1201 ctccagccag cgggcgaccg cgagggcgtt ctcgttgtgc gccttcatgc gcaggcccag
     1261 cgtcttcagc ccccggttgg cgaggaaggc gtcgaacggc cccatcaccc cgccaacgga
     1321 attctggagg aactttatct ctttcgcgag gtcggggttc gccgtcacca gcgccccgcc
     1381 gatgatgtcg gagtggccat tcagatattt ggtcgccgaa tgcatgacca cgtcggcgcc
     1441 cagcttcagc ggctgctggc tccacggcgt ggcgaaggtg ttgtcgacga tcagcagcag
     1501 gccatgcgcc ttggcgatcc gcgacaggcc ctcgatgtca gccaatttca tcagcgggtt
     1561 ggtcggggtc tcggcccaga tcagcttggt cttcggcgtg atcgccgcct cgaccttcgc
     1621 cagatcgctg aggtcgacat aggcgaagtc cagccccatg gaccggcgac gcacccgctc
     1681 gaacagccgc cacgacccgc cgtacagatc gtcgccggtg acgatgtgcg atcccgcgtc
     1741 cagcagttcc agcgccgtcg aggtcgccgc catgccgctg gcgaagccga agccgtgggt
     1801 cccgccttcc agatcggcca gacaggcctc gaaagcctcg cgcgtcgggt ttttgccgcg
     1861 ggcgtactca tagcccttgt tcacgcccgg gctttcctgg gcgtaggtcg aggtggcgta
     1921 gatgggcgtc attaccgccc ccgtcgtggg atctggccgc tgaccggcgt ggatggcgcg
     1981 ggtcgagaaa ccctggctgt tcttcagctt ggtcatcaat tgtcctggtc ggggcggccg
     2041 atgttgcccg ggtgacggct ttccatgaag gcgcgcgtcc tgctcatgac ggcgcaccag
     2101 gtcgacaggt cgtactgatg cccgtcgaag tccagaccct ggacctcgac cgcggcgccc
     2161 gcggcgcgca ggtcctcggc cagttccagg gtcgcgcgaa ccggaaccgc ctcgtcgcgg
     2221 cgcgcatgca gcagcaggaa ggacagatcg cggc
//
LOCUS       DASVVN010000547         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_232606,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000547 DASVVN010000000
VERSION     DASVVN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232606"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1037
                     /locus_tag="VF686_08875"
     CDS             186..1037
                     /locus_tag="VF686_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX8661969.1"
                     /translation="MAIGVFDSGVGGLTVHRELTRRFPDRDFVYLADQANAPYGGRGG
                     EEIVELTRAGCERLFEAGANVIVLACNTASAIALRRLQQTWVPEARARHGRPINVLGI
                     IVPTIEAATGLPWTYEAERRGEKIEAIDITGVFCTAATAMSRVYEIEIDKRREDLAVF
                     SEPCPGLAGLIELGAPADELKVVVEDHVDALRRRIGRHPDQAILGCTHYEIVADLFAA
                     ALPPGTPVIHQPTAVADALSRYFERHPEYDLGGSGRRDFLTSGKAGPQSDLVGQFWGA
                     PLTFTPA"
     gene            1041..1409
                     /locus_tag="VF686_08880"
     CDS             1041..1409
                     /locus_tag="VF686_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8661970.1"
                     /translation="MQAPADAADQLRAFGLTYPEVALKSPWPGHADLTVKDKTFAYLS
                     VAGEPFSLSCKLPFSAGEALAMSFVTPTGYGLGKSGWATASWPDDVEDPMALFRSWID
                     ESYRAQVPKKLIAQALAFPA"
     gene            1459..1977
                     /locus_tag="VF686_08885"
     CDS             1459..1977
                     /locus_tag="VF686_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661971.1"
                     /translation="MFKHLIAVAALALSATPAAAEVVERSADHFVLRYAVALEAVPAD
                     FIDALEHIGEWWNPAHTYSGDARNLSLALDAGTCFCEAMPDGSTFDHGGVYEYDPATG
                     VLIDAALGPLRGKTTLSNWSIGWTGANRGTELVMSYVVRGEGLGAMADGVDTVMGEQF
                     QRMVHFVEYGEP"
     gene            complement(1950..2585)
                     /locus_tag="VF686_08890"
     CDS             complement(1950..2585)
                     /locus_tag="VF686_08890"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX8661972.1"
                     /translation="MTRVVSTVMFGLMALLAMAVALVSARYFMPEVPFPAPAMVVQMA
                     KWPIAVLAHVGGGVAALALGSFQLVTRRGPRRIWHRWAGRTYVTACLVAAVSGFWLAL
                     HASAGPIATTGFGALAVVWLATTAMGWRKAVTGEFGQHRRWMIRSLSLTFAAVTLRIM
                     LPMIPLTGLTSDDGYRAVSFLCWIPNLLLVEVWLRTFGWESGSQGSPYSTK"
     gene            2688..3476
                     /locus_tag="VF686_08895"
     CDS             2688..3476
                     /locus_tag="VF686_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8661973.1"
                     /translation="MKAAGVIRRLDAFRLGRSGAAELVALALFGLFLAAIGPFGSDKT
                     PTASRYLYWQLAMIGGGVIAALIEPVIARSLTGRPRLFAVAQLAAMTLPIAVWVSVLP
                     VVMFGARLSAERILQVLPSVLTINIAVVVLAWLTRKALNRRDTPAAIPDGVAPPALRA
                     KLPPRLARARLIAVEAEDHYLRIRTEAGSALVLMRLGDALEALSGTDGFRTHRSWWVA
                     RSAVETARWKAGRGQLILSDGSAAPVSRTYAAALKGTDWAAPIA"
     gene            complement(3485..4018)
                     /locus_tag="VF686_08900"
     CDS             complement(3485..4018)
                     /locus_tag="VF686_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8661974.1"
                     /translation="MSIDSLRDLIPAYAKDISLNLGSLASETVLNDQQKWGTFLASAH
                     AIGVGPVVNAIEAQAATVLSPEAMSAAKSAAAIMGMNNIYYRSLHLMKNQEYTTIPAR
                     LRMNVIANPGVDKMDFELWSTAVSAINGCGACLDAHESELRKHGVPATQVQAALRIGA
                     VVHAASRIVASEAALAD"
     gene            complement(4104..>4487)
                     /locus_tag="VF686_08905"
     CDS             complement(4104..>4487)
                     /locus_tag="VF686_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8661975.1"
                     /translation="PTEIAEFARLNPQFEERDAVVLGGSTDNEFSKLAWRRDHPDLAN
                     LAIWSFADNGGKFARQLGVLNEEEGVALRATFVVDPHNIVQHVYVTNLNVGRAPADTL
                     RVVDALQTDELCACNRPVGGETLAA"
BASE COUNT          693 a         1519 c         1562 g          713 t
ORIGIN      
        1 agagcgcgaa catgctgccc ccggcgatga gaaggtgacg tctgtgcatg agaaactccg
       61 tgcgtggcgt tcgttccctg accctaggcg ctcgggccgc tgactgtcac atgaacggac
      121 cgtaatcttc gctggtggac atcggcggat ggacgggggc cggcggcaag tctaaagccc
      181 ccgccatggc gattggcgta ttcgactccg gcgtgggcgg gctgacggtc caccgcgagc
      241 ttacgcggcg gttccccgac cgggatttcg tctatctggc cgatcaggcc aacgccccct
      301 acggtggacg cggcggcgag gagatcgtcg aactgacccg cgcgggctgc gaacggctgt
      361 tcgaggccgg cgccaatgtc atcgtcctgg cctgcaacac cgccagcgcc atcgccctgc
      421 gccggctgca gcagacctgg gtgcccgagg cgcgggcgcg tcacggccgt ccgatcaatg
      481 tgctgggcat catcgtcccg accatcgagg cggccacggg cctgccgtgg acctatgagg
      541 ccgagcggcg cggagagaag atcgaggcca tcgacatcac cggcgtcttc tgcacggcgg
      601 ccaccgccat gagccgggtc tatgagatcg aaatcgacaa gcgccgcgag gatctggccg
      661 tcttctcgga gccttgcccc ggtctggccg gcctgatcga actgggcgcc ccggccgacg
      721 agctgaaggt ggtggtcgag gatcacgtcg atgccctgcg ccgccgcatc ggccgccatc
      781 cggaccaggc cattctcggc tgcacccact acgagatcgt cgccgatttg ttcgctgcgg
      841 ccctgccgcc cggcacgccg gtgatccatc agccgaccgc cgtggccgac gccctgtcgc
      901 gctatttcga acgccatccg gaatacgatc tgggcggctc gggccgccgc gacttcctga
      961 ccagcggcaa ggccggtccg cagtcggatc tggtcggcca gttctggggc gcgcccctga
     1021 ccttcacccc ggcctgatcc atgcaagcgc ccgcggacgc cgccgaccag ctgagggcct
     1081 tcggcctgac ctatcccgag gtcgccctga agagcccgtg gccgggtcac gccgatctga
     1141 cggtgaagga caagaccttc gcctatctga gcgtggcggg cgagccgttc agcctctcgt
     1201 gcaagctgcc gttctcggcc ggggaggcgc ttgccatgtc gttcgtgacg cccaccggct
     1261 acggcctcgg caagagcggc tgggcgaccg cgtcatggcc ggacgatgtc gaggatccga
     1321 tggccctgtt ccgcagctgg atcgacgaga gctaccgcgc ccaggtgccg aagaagctga
     1381 tcgcccaggc gctggccttc ccggcctgac ttgacgacct ccgcttttcg tcgcccgatg
     1441 gcgtcgggag gaagtccgat gttcaaacac ctgatcgccg tggccgcgct ggcgctgtcg
     1501 gccacgcccg ccgccgcgga agtggtcgaa cgcagcgccg atcatttcgt cctgcgctac
     1561 gccgtcgcgc tggaggccgt tcccgcggac ttcatcgatg cgctggaaca catcggcgaa
     1621 tggtggaacc cggcccacac ctacagcggc gacgcccgca acctgagcct cgcgctcgat
     1681 gcgggaacct gcttctgcga ggccatgccg gacggatcca ccttcgacca cggcggagtc
     1741 tatgaatacg acccggcgac cggcgtcctg atcgacgccg cgctgggacc gttgaggggc
     1801 aagacgaccc tgtccaactg gtccatcggc tggacgggcg ccaatcgcgg cacggagctg
     1861 gtgatgtcct acgtcgttcg cggcgaaggc ctcggcgcca tggccgacgg cgtcgacacg
     1921 gtcatgggcg aacaatttca gcgcatggtt cacttcgtcg aatacggcga gccctgagaa
     1981 ccggattccc aaccgaaggt cctgagccag acctcgacca gcagcaggtt gggaatccag
     2041 cacaggaagg agacggcgcg gtagccgtcg tcggacgtca ggccggtcag cgggatcatc
     2101 ggcagcatga tcctgagcgt caccgccgcg aaggtcagcg acaggctgcg gatcatccac
     2161 cggcgatgct ggccgaactc gcccgtcacg gccttgcgcc aacccatggc ggtggtcgcg
     2221 agccagacga cggccagcgc gccgaagccg gtcgtggcga tgggcccggc cgaggcgtgc
     2281 agcgccagcc agaagccgga gacggccgcg accagacagg ccgtcacata ggtccggccc
     2341 gcccagcgat gccagatccg ccggggcccg cggcgcgtga ccagctggaa cgagcccagc
     2401 gccagagccg cgaccccgcc gccgacatgg gccagcaccg cgatgggcca tttggccatc
     2461 tggacgacca tcgccggcgc cggaaaggga acctcgggca tgaagtagcg ggcgctgacc
     2521 agcgccacgg ccatcgccag caaggccatc aggccgaaca tcaccgttga aaccactctc
     2581 gtcacgtctt ccctccgccg ttgagccgta tcgcttgaac cgggccgcgc aggcgtgtca
     2641 gccttgtctt cgcgaacgga cggctccgtt cgcgaacggg gcgcggcgtg aaggcagcag
     2701 gggtcatcag acggctggac gcgttccggc tgggacggtc gggggcggcc gaactggtcg
     2761 ccctggcgct gttcggcctg ttcctggccg cgatcggacc tttcggttcc gacaagacgc
     2821 cgacagcgag ccgctatctg tactggcaac tggcgatgat cggcggcggc gtgatcgcgg
     2881 ccctgatcga gccggtgatc gcccgctcgc tgacggggcg gccgcggctg ttcgccgtcg
     2941 cccagctggc ggcgatgacg cttcccatcg ccgtctgggt gtctgtcctg ccggtggtca
     3001 tgttcggggc gcggctgtca gccgagcgga tcctgcaggt cctgcccagt gtgctgacca
     3061 tcaatatcgc cgtcgtggtt ctggcctggc tgacccgcaa ggcgctgaac cgcagggaca
     3121 cgccggcggc gattccggac ggggtcgccc cgcccgccct gcgcgccaaa ctgccgcccc
     3181 ggctggcgcg ggcgcggctg atcgcggtcg aggccgagga ccactatctg cgcatccgca
     3241 ccgaggccgg ctcggccctg gtgctgatgc ggctgggcga tgcgcttgag gcgttgtcgg
     3301 gcacggacgg gttccgcacc catcgctcgt ggtgggtggc ccgctcggcc gttgagaccg
     3361 cgcgatggaa ggccgggcga ggtcagctga tcctgtcgga cgggtcggcg gcgccggtca
     3421 gccggaccta cgccgccgcc ctgaagggca cggactgggc cgcgcccatc gcctgatttg
     3481 gcgtctagtc cgccagggcg gcttccgacg ccacgatacg gctggcggcg tgaaccaccg
     3541 cgccgatgcg cagcgcggcc tgcacctgcg tcgccggcac cccgtgcttg cgcagttcgc
     3601 tctcgtgggc gtccagacag gcgccgcagc cgttgatcgc cgacaccgcc gtcgaccaca
     3661 gctcgaagtc catcttgtcg acgccgggat tggcgatgac gttcatgcgc aggcgagcgg
     3721 ggatggtcgt gtattcctgg ttcttcatca ggtgcagcga ccggtagtag atgttgttca
     3781 tgcccatgat ggcggcggcg gacttggccg cgctcatggc ctcaggcgac aggacggtgg
     3841 cggcctgggc ctcgatcgca ttgaccacgg ggccgacgcc gatcgcgtga gccgaggcca
     3901 ggaaagtccc ccatttctgc tggtcgttca gcaccgtttc ggacgccagc gagccgaggt
     3961 tcagggagat gtccttggcg taggcgggaa tgaggtcgcg aaggctgtcg atcgacatgg
     4021 gaaagtcctc gaaaatctga tctggatacg aaacaggcgg cggctcgcgc cgccgcctgt
     4081 ccagttcgac gctggccagt actttacgcg gccagggtct cgccgcccac cgggcggttg
     4141 caggcgcaca gctcgtcggt ctgcagggcg tcgacgacac gcagggtgtc ggcgggggcg
     4201 cggcccacgt tcaggttggt gacatagacg tgctgcacga tgttgtgcgg atcgacgacg
     4261 aaggtggcgc gcagggcgac gccctcttcc tcgttgagca cgccgagttg gcgggcgaac
     4321 ttgccgccgt tgtcggcgaa cgaccagatg gccaggttgg ccagatccgg gtggtcacgc
     4381 cgccaggcga gcttggagaa ctcgttgtcg gtggagccgc ccagcaccac ggcgtcgcgc
     4441 tcctcgaact gcgggttcag gcgggcgaac tcggcgatct cggtcgg
//
LOCUS       DASVVN010000548         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_21229, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000548 DASVVN010000000
VERSION     DASVVN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21229"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="fni"
                     /locus_tag="VF686_08910"
     CDS             <1..776
                     /gene="fni"
                     /locus_tag="VF686_08910"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX8661976.1"
                     /translation="AAQHLGIGLAVGSQRAALESDGAPGLDMALRLKAPDTPILANIG
                     AAQLTRGFGVDEARRVLDMIAADALIVHLNPLQEACQPEGDRDWWGVGAALEALVKAL
                     DAPVVVKETGAGMSAATAKRLLAMGVAAVDVAGAGGSNWATVEGERAEGQADKAHAAA
                     FADWGIPTARSIADVRAACPKALIIGSGGIRDGVEAAKAIRLGADMVGQAAGVLTAAT
                     ISTEAVVEHFQTLIRQMRTVCFCTGSANLTALRKAPLQG"
     gene            complement(939..>1610)
                     /locus_tag="VF686_08915"
     CDS             complement(939..>1610)
                     /locus_tag="VF686_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX8661977.1"
                     /translation="PQVMGAVLDVLKSAAATLECEANAVTDNPLVFIDDGDVISGGNF
                     HAEPVAFAADMIAMALCEIGNISERRTSILVDPKMSELPAFLVKESGLNSGFMIAQVT
                     AAALVAENRMVAHPCSIDTVPTSANQEDHVSMATHGARRLLDMAENTAAVVGIELLAA
                     AQGIEFHRPLKSSPELEKVWAIVREAAAAYGSDHYFAPDIARATDGVRAGRYRAFAGD
                     LLPSA"
BASE COUNT          250 a          512 c          612 g          236 t
ORIGIN      
        1 tggcggcgca gcatctgggg atcggcctgg cggtcggttc gcagcgtgcc gcgcttgaga
       61 gcgacggggc gccggggctg gacatggcgc tgaggctgaa ggcgcccgac acgccgatcc
      121 tggccaatat cggcgcggcc cagctgacgc ggggcttcgg cgtcgacgag gcgcgacggg
      181 tgctggacat gatcgccgcc gacgccctga tcgtacacct gaacccgttg caggaggcct
      241 gccagcccga gggcgaccgc gactggtggg gcgtgggcgc ggcgctggag gccttggtca
      301 aggcgctgga cgcgccggtg gtggtcaagg agacgggcgc cggcatgtcg gcggcgacgg
      361 cgaaacggct gctggccatg ggcgtcgcgg cggtggatgt ggccggagcc ggcggttcga
      421 actgggccac ggtcgagggc gagcgggcgg aagggcaggc ggacaaggcc cacgccgcgg
      481 ccttcgccga ctggggcatc cccacggccc ggtccatcgc cgacgtccgg gcggcctgtc
      541 ccaaggccct gatcatcggc tcgggcggga tccgcgacgg ggtcgaggcg gccaaggcga
      601 tccggctggg ggccgacatg gtcggtcagg cggcgggggt gctgacggcc gcgacgatct
      661 cgaccgaggc ggtggtcgag catttccaga ccctgatccg ccagatgcgc acggtctgtt
      721 tctgcaccgg ctcggccaat ctgacggccc tgcgcaaggc gccgctgcag ggctgacccg
      781 cggtctgcag ccgggaacac agtgcgaagc gtgcgctccg tgcgccggaa aaaacaccgt
      841 ccgaagagtc cgaagagtcc actcagtcca cttcggcaga accgattgcc gaccaccaac
      901 gcactctacc gccttcgcgc gtcaaaatcg gtcgtacgtc aggccgacgg cagcagatca
      961 cccgcgaacg cccggtaacg tcccgcccgc accccgtccg tcgcccgggc gatgtctggc
     1021 gcgaaatagt ggtcggagcc gtaggcggcg gccgcttcgc gcacgatggc ccagaccttt
     1081 tccagctcgg gcgaggactt cagcggccgg tggaattcga tcccctgggc ggccgccagc
     1141 agctcgatgc ccaccacggc ggcggtgttc tcggccatgt ccagcaggcg gcgggcgccg
     1201 tgggtggcca tggaaacgtg gtcttcctga ttggcggagg tcgggacggt gtcgatgctg
     1261 cacggatggg cgaccatccg gttctcggcg acgagggcgg cggcggtgac ctgggcgatc
     1321 atgaagccgg agttgaggcc ggattccttg acgaggaagg cgggcagttc gctcatcttc
     1381 ggatcgacga ggatggaggt gcggcgctcg gaaatattgc cgatctcgca cagggccatg
     1441 gcgatcatgt cggcggcgaa ggcgaccggc tcggcgtgga aattgccgcc cgaaatgacg
     1501 tcgccgtcgt cgatgaagac caacggattg tcggttacgg cgttggcctc gcattccagc
     1561 gtggcggcgg cgctcttcag cacgtcgaga acggcgccca tgacctgcgg
//
LOCUS       DASVVN010000549         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_257457,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000549 DASVVN010000000
VERSION     DASVVN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257457"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /gene="ptsP"
                     /locus_tag="VF686_08920"
     CDS             <1..1823
                     /gene="ptsP"
                     /locus_tag="VF686_08920"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX8661978.1"
                     /translation="EDIQTIAMVLAETVASGELLAQDELRDIEVAPHRPERLKGQRFA
                     EGLAYGHVILHEAPVPPENLLAENQQVEEIRLREALIVLKANIDSILDGGQGKLSGQP
                     FEVLETYRMFADDRGWNRSLEEAVRTGLTAEAAVDRVRNEHRARFAQARDPYIKERLH
                     DFEDLANRLLRVLAGDKPGERDLPDDAILVARNLGPADLLEYPRHKLRGLLLEEGSAA
                     SHAAIVARALQIPCVGRIMGLRDRLSEGDLVIVDGETGETYLRPRPDMLAAVQSRMLV
                     RSERQAEFAKLRDVPAVTLDGTRITVLTNAGLAVDLENLDATGAEGIGLFRTEFQFMV
                     SEELPRLTSQTALYKLVLDTAGDRPVTFRTLDIGGDKVLPYLETAREENPALGRRAIR
                     LGLDRPGLLRLQLRALLAAAAGRELRVMFPMIATVDEFRAARELVDVECAWARRRGRA
                     LPALLRVGAMIECPSLLWHLDALLPLTDFVSIGTNDLFQYMFAADRTNPLVSERYDPL
                     SPPALRALAEIQKKCADTGTPVSVCGELAGKPLEAFALLTLGYTRLSAPAGGVGPVKR
                     MILSADLGAARRGMANLLGSSAGSIRGELESLARKLNVTI"
     gene            2019..>3027
                     /locus_tag="VF686_08925"
     CDS             2019..>3027
                     /locus_tag="VF686_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8661979.1"
                     /translation="MALDTGNGSDEFRAHPDWKDVVPSITDAPTLGDGLRVARKQSGR
                     SLAELSAVTRVPARYLTALEQNDFSTLPNRVFSIGYVRAYADALGLDEQLAVERFKRE
                     TPDPSVPLQAPVGVAFEDVRRYSPRLIAAGLALIVAVVGWNVFQRVSLMRAPQPSDIA
                     SVPESWTLGEVPGQSLRLSAPQPAPPDQTIPALYVTPGLEAELTGVDPTSPEGLAAAA
                     ASAASPVQRAFNPRGAIYGASASASQVVIQARKAASLVVRMADGQVLFARQLAPGDAW
                     RAPVGLSAVVDASSDFADFDVYLNGEHGGTLTAEQTPLSQLNARAQTLARQAAAEVAA
                     RT"
BASE COUNT          437 a         1162 c          975 g          453 t
ORIGIN      
        1 tcgaggacat ccagaccatc gccatggtgc tggccgagac ggtggcgtcg ggcgagctgc
       61 tggcccagga cgagctgcgc gacatcgagg tggccccgca ccgccccgaa cgcctcaagg
      121 ggcagcgctt cgccgagggc ctcgcctatg gccacgtcat cctgcacgag gcgccggtgc
      181 cgccggagaa cctgctggcc gagaaccagc aggtcgagga aatccgcctg cgcgaggcgc
      241 tgatcgtcct caaggccaac atcgactcca tcctcgacgg gggtcagggc aagctgtccg
      301 gccagccgtt cgaggtgctg gaaacctacc gcatgttcgc cgacgaccgg ggctggaacc
      361 ggtcgctgga ggaggcggtg cgcaccggcc tgaccgccga ggccgccgtc gatcgggtcc
      421 gcaacgaaca ccgcgcccgc ttcgcccagg cccgcgaccc ctacatcaag gaacggctgc
      481 acgacttcga ggacctggcc aaccgtctgc tgcgggtgct ggccggcgac aagcctggcg
      541 agcgcgacct gccggacgac gccatcctcg tggcccgtaa cctcggcccc gccgacctgc
      601 tggagtatcc gcgccacaag ctgcggggcc tgctgctgga agaaggctcg gccgccagcc
      661 acgccgccat cgtcgcccgc gcgctgcaga tcccgtgcgt cggccgcatc atgggccttc
      721 gcgaccgcct gtccgaaggc gatctggtca tcgtcgacgg cgagaccggc gagacctatc
      781 tgcgcccccg ccccgacatg ctggccgccg tccagtcgcg catgctggtg cgcagcgagc
      841 gtcaggccga gttcgccaag ctgcgcgacg tgcccgccgt caccctcgac ggcacgcgca
      901 tcaccgtcct gaccaacgcc ggtctggccg tcgatctgga aaacctcgac gccaccgggg
      961 ccgagggcat cggcctgttc cgcaccgaat tccagttcat ggtctccgag gaactgcctc
     1021 ggctgaccag ccagacggcc ctgtacaagc tggtgctcga caccgccggc gaccgcccgg
     1081 tcaccttccg cacgctggac atcggcggcg acaaggtcct gccctatctc gagacggcgc
     1141 gcgaagagaa cccggcgctg ggccgccgcg ccatccgtct cggcctcgac cggcccggcc
     1201 tgctgcggct tcagctgcgc gccttgctgg ccgccgccgc cggacgcgag ctgcgcgtca
     1261 tgttccccat gatcgccacg gtggacgagt tccgcgccgc ccgcgaactg gtcgatgtcg
     1321 aatgcgcatg ggcccgacgc cggggccgcg ccctgccggc cctgctccgc gtcggggcca
     1381 tgatcgaatg tcccagcctg ctgtggcacc tcgacgccct gctgccgctg acggatttcg
     1441 tctcgatcgg caccaacgat ctgttccagt acatgttcgc cgccgaccgc acgaacccgc
     1501 tggtgtccga acgctacgac cccctgtcgc cgccggccct gcgcgccctg gccgagatcc
     1561 agaagaaatg cgccgacacc ggcacgcccg tctccgtctg cggcgaactg gccggcaagc
     1621 cgctggaggc cttcgccctg ctgacgctgg gctacacccg tctgtcggcc ccggccgggg
     1681 gcgtcggacc ggtcaagcgg atgatcctgt cggccgacct cggcgccgcg cggcggggca
     1741 tggccaatct gctcggctcg tccgccggat cgatccgcgg cgaactggaa tccctggccc
     1801 ggaagttgaa cgtcacgatc tgatcgttcc gcgccgaacc cggccggtac gcctaacaaa
     1861 tcgttgatcg gtcagctcta atgagttatc cacaagtcgg aaccgggtgg tctgcggggg
     1921 caggcccgaa ctgtcatccc gatatggtac aggccccgag ttcgagtacg gcgccgcatg
     1981 gggacactcc gtgcgcgccg agtgtggggt caggcgccat ggcgttggat acaggcaacg
     2041 ggtccgacga gttccgggct caccctgatt ggaaggacgt cgttccttcg atcacggacg
     2101 ccccgactct gggcgacggt cttcgcgtgg cccgcaagca gtcgggccgg tcgctggccg
     2161 aactgtccgc cgtcacccgc gttcccgccc gctatctgac cgcgctggaa cagaacgact
     2221 tctcgaccct gcccaaccgc gtcttctcca tcggctacgt ccgcgcctac gccgacgccc
     2281 tggggctgga cgaacagctg gcggtcgaac gcttcaagcg cgagactccc gacccctcgg
     2341 tgccgcttca ggcgccggtc ggcgtcgcct tcgaggatgt ccgccgctat tcgccccgcc
     2401 tgatcgccgc cggcctggcc ctgattgtcg ccgtcgtcgg ctggaacgtc ttccagcgcg
     2461 tcagcctgat gcgcgcgccc cagccctctg acatcgcctc agtgcccgaa agctggaccc
     2521 tgggcgaggt cccgggccag tcgctgcgcc tgtcggcgcc gcaaccggcc ccgcccgacc
     2581 agaccattcc ggccctgtat gtcacgcccg gtctcgaggc cgagctgacg ggcgtcgacc
     2641 cgacctcgcc cgagggcctg gccgcagccg ccgcctcggc ggcctcgcct gtccagcgcg
     2701 ccttcaaccc tcgcggcgcc atctacggcg cctcggccag cgcctcacag gtcgtgatcc
     2761 aggcccgcaa ggccgccagc ctcgtggttc gcatggccga cggccaggtc ctgttcgccc
     2821 gccaactggc tcccggcgac gcgtggcgcg cgccggtcgg cctgtccgcc gtggtcgacg
     2881 cctcgtccga cttcgccgat ttcgacgtct atctgaacgg cgagcacggc ggcacgctga
     2941 cggccgaaca gacaccgctc agccagttga acgcccgcgc ccagaccctg gcccgtcagg
     3001 ccgccgccga ggtcgccgcc cgcaccc
//
LOCUS       DASVVN010000550         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_520750,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000550 DASVVN010000000
VERSION     DASVVN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520750"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VF686_08930"
     CDS             <1..862
                     /locus_tag="VF686_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX8661980.1"
                     /translation="QAVEAAPAATARVVPQAPGAGPALWAIRDADSTIYLFGTVHVLR
                     PTTPWGSARVDAAFAGADDIWFEISNPDDQAAIIPLVQQHGISPDRPLSSLLTAEELT
                     DLSAAAAGAGMTAAQIDIFRPWLAGLTLSVAPLIKAGYDPQSGVEMILKARADAAGKP
                     VHGFETIDKQVGILAGMSEADQLSFLRSTLEAYDDATTELDAMVDAWATGDVATLEKV
                     SVEEMQAESPAVYQALLVQRNTDWAGQIQTLLAGSGTAFIAVGAAHLVGDDSVQEILA
                     DRGVAVTREE"
     gene            complement(872..945)
                     /locus_tag="VF686_08935"
     tRNA            complement(872..945)
                     /locus_tag="VF686_08935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(911..913),aa:Gly,seq:ccc)
     gene            complement(995..2653)
                     /locus_tag="VF686_08940"
     CDS             complement(995..2653)
                     /locus_tag="VF686_08940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8661981.1"
                     /translation="MIRTLALTLLLAGAAASAEAQDMLIKGGPIYTGVDAAPTAEAVL
                     VRDGRIAWVGRLADQPAVPTEVPTIDLKGAALFPGFTDGHAHLDGVGWRELTLNLEGS
                     ASVADAMARLTAWAAAHPDGPIVGRGWIETRWPEARFLTAADLDAAAPGRVVLLSRAD
                     GHAVVASSPALAAARIDASTVAPSGGEILKAPDGQPTGLLVDAAEQLVARLMPQADPA
                     ALREAYRAGFRVEAAYGWTGVHFMSAPWKDIPLLEDMAADGEAPLRIYNSVTPDGAAE
                     LIAGGPRSVADGRIITRAIKYYADGALGSRGAALFEPYADRPDTTGLMQITEQEIVPL
                     YQAALRGGIQIATHAIGDRGNASVAEWYQQALAGVAPGERPNGADVRWRIEHAQILRP
                     SDYHWFKDLPIIASMQPSHAIGDLYFAPARLGDARLDGAYAWHSLADLGVIVVGGSDA
                     PVERGSPLIEFYAAVARADLEGHQGPDWRPNEAVDRATALKMFTLWPAYASFREAELG
                     TIEVGKRADLTAFDVDLMTAPFADIPKGEATLTVVDGVVVYRAD"
     gene            2736..>3150
                     /locus_tag="VF686_08945"
     CDS             2736..>3150
                     /locus_tag="VF686_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX8661982.1"
                     /translation="MKLYTIGYEGEPQAAVIDRLKAAGVTVLADVRAVAASRRAGFSK
                     TVLGSSLAEAGIEYVHLRDLGTPKAGRDAARKGRIGEMRGIFASHMEEPPSQLAFERL
                     REIAKDRPTALLCFEADHAGCHRAVLAERLASEDGF"
BASE COUNT          471 a         1109 c         1138 g          432 t
ORIGIN      
        1 acaggcggtc gaggccgcgc ccgccgccac cgcccgcgtc gtgccccagg cccccggcgc
       61 cggcccggcg ctgtgggcca tccgggacgc cgactcgacc atctacctgt tcggcaccgt
      121 ccacgtgctg cgtccgacga cgccctgggg ctccgccagg gtcgacgccg ccttcgccgg
      181 cgcggacgac atctggttcg agatctccaa tcccgacgat caggcggcca tcatcccact
      241 cgtccagcag cacggcatct cgcctgaccg gcccctgtcc agcctgctga cggccgagga
      301 gctgaccgac ctgagcgccg ccgccgccgg cgccggcatg accgccgccc agatagacat
      361 tttccggcct tggctggccg gcctgaccct ctcggtcgca cccctgatca aggccggcta
      421 cgatccccag tcgggcgtcg agatgatcct caaggcccgc gccgacgccg ccggcaagcc
      481 ggtccacggc ttcgagacca tcgacaaaca ggtcggcatc ctggccggca tgtcggaggc
      541 cgatcagctg tccttcctgc gctcgacgct ggaagcgtat gacgacgcca ccaccgagct
      601 ggacgccatg gtcgacgcct gggccaccgg cgatgtcgcg acgctggaga aggtctcggt
      661 cgaggagatg caggccgaat ccccggccgt ctatcaggcc cttctggtcc agcggaacac
      721 cgactgggcg ggccagatcc agacactgct ggcggggtcg ggcaccgcct tcatcgccgt
      781 cggcgcggcc cacctcgtgg gcgacgacag cgtgcaggaa atccttgcgg accggggcgt
      841 cgccgtcacc cgcgaggagt agggcgacgg ctggagcggg cggcgggaat cgaacccgca
      901 cgaaaagctt gggaagcttt caggctacca ctacatcacg cccgcgcagc cgccgcagcc
      961 ttagggccaa cggcggaccg catcaagacg gggatcagtc ggcgcgatag acgacgaccc
     1021 cgtcaaccac cgtcagcgtc gcctcgccct tggggatgtc ggcgaagggc gcggtcatca
     1081 ggtcgacgtc gaaggccgtc agatcagccc gcttgccgac ttcgatcgtg cccagctcgg
     1141 cctcgcggaa gctggcgtag gccggccaca gcgtgaacat cttcagggcc gtggcgcgat
     1201 ccaccgcctc gttcggacgc cagtcggggc cctgatggcc ctccagatcg gcgcgggcga
     1261 cggcggcgta gaactcgatc aggggggagc cccgctcgac cggcgcgtcg gaaccgccga
     1321 caacgatcac gcccaggtcg gccaggctat gccatgcgta ggccccgtcc agccgcgcgt
     1381 cgccgagacg ggcgggcgcg aaatacaggt cgccgatggc gtggctgggc tgcatcgagg
     1441 cgatgatcgg caggtccttg aaccagtgat agtcagacgg gcgcagaatc tgggcgtgct
     1501 cgatccgcca gcgcacgtcg gcgccgttgg gacgctcgcc cggcgccacg ccggccagcg
     1561 cctgctggta ccactccgcc accgaagcgt tgccccggtc gccgatggcg tgggtcgcga
     1621 tctggatgcc gcccctcagc gccgcctggt acagcggcac gatctcctgt tccgtgatct
     1681 gcatcaggcc ggtggtgtcg ggccggtcgg cgtatggttc gaacagcgcc gcgccgcgcg
     1741 aacccaaggc tccgtcggca tagtatttga tcgcccgggt aatgatccgg ccgtcggcga
     1801 cgctgcgcgg gccgcccgcg atcagctcgg ccgcgccgtc gggcgtgacg ctgttgtaga
     1861 tgcgtagcgg agcctcgccg tccgccgcca tgtcttccag cagggggatg tctttccacg
     1921 gcgcgctcat gaagtggacg ccggtccagc cataggcggc ctcgacgcgg aagccggcgc
     1981 ggtaggcttc ccgcagggcc gcggggtccg cctgaggcat cagccgggcg accagttgct
     2041 ctgcggcgtc gaccagcaga cccgtcggct ggccgtcggg ggccttgagg atttcaccgc
     2101 ccgaaggggc gaccgtcgat gcgtctatgc gggcggcggc gagggcgggg ctggaagcga
     2161 cgaccgcatg gccgtcggcc cggctcaaca gcacgacccg gcccggcgcg gcggcgtcca
     2221 gatccgcggc ggtcaggaag cgggcctcgg gccagcgggt ctcgatccag ccgcgtccga
     2281 cgatggggcc gtccgggtgt gcggccgccc aagcggtcaa ccgggccatg gcgtcggcga
     2341 cagaggccga cccttcgagg ttcagggtca gctcgcgcca gccgacgccg tcgaggtggg
     2401 cgtgaccgtc ggtgaagccg ggaaacaggg cggcgccctt caggtcgatg gtcgggacct
     2461 ccgtcgggac cgcgggttgg tcggccagtc ggccgaccca cgcgatccgc ccgtcgcgga
     2521 ccagcaccgc ctcggcggtc ggggcggcgt ccacgcccgt atagatgggc ccgcccttga
     2581 tcagcatgtc ctgcgcctcg gccgaggctg cggccccggc gagcagaagg gtcagggcga
     2641 gggtgcggat catgacgcgg ctccggttga ggcgaccgga ggggtggagc cgaaagccgc
     2701 tggcgtcaag cggatgtcgg acccatgtgg gaggcatgaa actctacacc atcggatacg
     2761 agggcgagcc gcaggccgcc gtcatcgacc ggctcaaggc cgcgggcgtg accgtgctgg
     2821 ccgatgtgcg ggcggtggcg gcgtcgcgcc gggccggctt ttcgaagacc gtgctgggct
     2881 cgagcctggc cgaggccggg atcgaatacg tccacctccg cgacctcggc acccccaagg
     2941 ccgggcggga cgcggcgcgc aagggccgga tcggcgagat gcgcggcatc ttcgccagcc
     3001 atatggagga accgccgtcg caactggcct tcgagcggct gcgggagatc gcgaaggacc
     3061 ggcccacagc gctgctgtgc ttcgaggccg accacgcggg ctgtcaccgc gcggtgctag
     3121 ccgagcggct ggcgagcgag gacgggttcg
//
LOCUS       DASVVN010000551         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_494971,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000551 DASVVN010000000
VERSION     DASVVN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494971"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VF686_08950"
     CDS             complement(<1..1032)
                     /locus_tag="VF686_08950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8661983.1"
                     /translation="MSKTFDSFGLNPALLQALASEGYTTPTPIQAQAIPDVMAGKDLL
                     GIAQTGTGKTAAFALPILHRLAANRIPPVPRTTRVLILSPTRELASQIAESFKSYGKH
                     LGFRVAVIFGGVKYGPQERALQQGLDVLVATPGRLLDHMQQKTLDLSQTEIFVLDEAD
                     QMLDLGFVKPIRQIVSRIPAKRQNLFFSATMPTEIGKLAGELLKDPVKVSVTPQATTV
                     QRINQSIIHIEQGRKRALLTEMFSDPHFTRCLVFTKTKHGADKVAAYLEAGGVEAGAI
                     HGNKSQSQRERALEAFKKGKLRVLVATDIAARGIDVDNVSHVVNFELPFVAEAYVHRI
                     GRTARAGAEG"
     gene            complement(1316..>1785)
                     /locus_tag="VF686_08955"
     CDS             complement(1316..>1785)
                     /locus_tag="VF686_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX8661984.1"
                     /translation="DEAGVAVLPLALFAALASIAVVVGEAVRVAGDAGQDGAAVLALV
                     TVALSWTFVHAIFAFHYAHEFYAPAEPDSAPGKGKGQDRGGLIFPGGEDPDYWDFVHF
                     STIIGVAQQTADIQISDRALRRTATVHSVTAFLFNTVIVALTVNMAVSLLGGG"
BASE COUNT          287 a          539 c          651 g          308 t
ORIGIN      
        1 gccctcggcg ccggcgcgcg cggtgcggcc tatgcggtgg acataggctt cggccacgaa
       61 aggcagctcg aagttgacca cgtgcgagac gttgtcgacg tcgatgccgc gcgccgcgat
      121 gtcggtggcg acgaggacgc gcagcttgcc cttcttgaag gcctcaagcg cccgctcgcg
      181 ttgcgactgg ctcttgttgc cgtggatggc gccggcttcg acgccgccgg cctcgaggta
      241 ggccgcgacc ttgtccgcgc cgtgcttggt cttggtgaag accaggcagc gggtgaagtg
      301 cgggtcgctg aacatctcgg tcagcagggc gcgcttgcgg ccctgttcga tgtggatgat
      361 cgactggttg atgcgctgaa ccgtggtggc ctggggcgtc accgacacct tgaccgggtc
      421 cttcagcagt tcgcccgcca gcttgccgat ctcggtcggc atggtggccg agaagaacag
      481 gttctgacgc ttggccggga tgcggctgac gatctggcgg atcggcttga cgaagccgag
      541 gtccagcatc tggtcggcct cgtcgaggac gaagatttcc gtctggctga ggtccagggt
      601 cttctgctgc atgtggtcga gcaggcggcc gggggtggcg accagaacgt cgaggccctg
      661 ttgcagggcg cgttcctgcg gcccgtactt cacgccgccg aagatgacag cgacccggaa
      721 gcccaggtgt ttgccgtagc tcttgaagct ttcggcgatc tggctggcca gttcgcgggt
      781 cggcgacagg atgagcacgc gcgtcgtacg gggcacgggc gggatgcggt tggcggccag
      841 acggtgcagg atcggcaggg cgaaggccgc cgtcttgccg gtgccagtct gggcgatgcc
      901 cagcaggtcc ttgccggcca tgacgtcagg aatggcctgc gcctggatcg gggtgggggt
      961 ggtgtagcct tcggaggcca gcgcctgcag cagggccggg ttcaggccga aggagtcgaa
     1021 tgtcttgctc atgtgagcgg cttctttcgc gtatgcggcg acgcgcatca gccatggctc
     1081 aacgccgaaa ggccgcgcac cgccagtccc gacagggtct caaaaaggag accttcgggg
     1141 tggatcgggc gccgccccgc gtgtccgggc agcgaatgaa tcttgaggaa ccggcgactg
     1201 ctacagtcag gtcttcgtcg ttcggaagac ccacacgcgc tggaggcgcc ggtaatcgca
     1261 attgtgcgac gcagcgtaag tgggctttca atgcggccaa gtcaaggcgc ggaggttaac
     1321 cgccacccag aaggctgacc gccatgttca ctgtcagcgc gacgataacg gtgttgaaca
     1381 ggaaggcggt gacgctgtgc acggtcgcgg tgcgacgcag cgccctgtcc gaaatttgaa
     1441 tgtcggccgt ctgctgggcg acgccgatga tcgtcgagaa atgcacgaag tcccagtagt
     1501 cggggtcttc gccgccgggg aagatcaggc cgccgcgatc ctgtcccttg cccttgcccg
     1561 gcgcactgtc cggctcggcg ggggcgtaga attcgtgggc gtaatggaag gcgaaaatgg
     1621 cgtgcacgaa ggtccaggac agggccacgg tgaccagtgc gaggactgcc gctccgtcct
     1681 gcccggcgtc gccggccacg cgcacggcct cgcccacgac cacggcgata ctggccaggg
     1741 ccgcgaacag ggccagcggc aggacggcga cgccggcctc atcca
//
LOCUS       DASVVN010000552         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_74379, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000552 DASVVN010000000
VERSION     DASVVN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74379"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VF686_08960"
     CDS             <1..720
                     /locus_tag="VF686_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8661985.1"
                     /translation="LLAVIGLSVFISRSTASVVMKKKSFWLLALGAASSSVCGYGVAG
                     WLPLFFMRSFGLTLTQVSWYYSGIALVGGIAGIWLGGMVADKLGKRSKGAYPLVPAIA
                     FLISAPCFILAMNSGWLIEQVMPGAGGNSFAALTMAFLIFLIPTGLNLVWLGPITAAV
                     QHLVPAPMRTTASALFLLINNLLGIAVGFFYFGWMSDLLAPRFGDESLRWAIYTGMGF
                     YLLASALLIGASRTLKKDWVE"
     gene            897..2087
                     /gene="sucC"
                     /locus_tag="VF686_08965"
     CDS             897..2087
                     /gene="sucC"
                     /locus_tag="VF686_08965"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX8661986.1"
                     /translation="MNIHEYQAKQVLKGFGAPVAEGVAITSVDQAEAAARSLPGPLYV
                     VKSQIHAGGRGKGKFKELGPDAKGGVRLAFSIEDVIKNAKDMLGNTLVTAQTGDAGKQ
                     VNRLYIEDGADIARELYCSLLVDREHGRIAYVASTEGGMDIEAVAHDTPEKIQTIVID
                     PATGVTEADVAKLNAAFELTGEAAEDGKTLFPLLYKAFVDTDMSMLEINPLIVMENGR
                     LRVLDAKVSFDGNALFRHPEIKELRDETEEDAKEIEASEWDLAYVALDGNIGCMVNGA
                     GLAMATMDIIKLYGKEPANFCDVGGGAGKEKVAAAFKIITADPAVQGILVNIFGGIMK
                     CDVIAEGVVAAVKEVGLKVPLVVRLEGTNAELGKKILNESGLTIQAADDLDDAAQKIV
                     AAVG"
     gene            2089..2439
                     /locus_tag="VF686_08970"
     CDS             2089..2439
                     /locus_tag="VF686_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661987.1"
                     /translation="MTRAPSLAILLIAACAATAAGAQTAPRGDMRITRVTDPVLGCTL
                     ITTSEVSRSGAGNVTLGRARTSREGETTIVSWSRVRVEGQRDRKPAVCRAEPGAITSV
                     TWGGVELLARPQPF"
     gene            2442..3032
                     /locus_tag="VF686_08975"
     CDS             2442..3032
                     /locus_tag="VF686_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661988.1"
                     /translation="MKSLRLLSVLISALAAASPSIAQDGPEAAPTDLIATLNTVCVAA
                     RGDRARAAALAAEAGFSPAPESMTPRMRNSSETAGFMRSSAADLTVIMTGTMTRRVGR
                     DTVVMEFCGVSARPTDHRALDARLLDLMNFPSVRGAGMEAYAWLQTPEGRVPLRSRRL
                     TDPQFLAMAETGQMRLVALDRSGPGSTLIYFLPRLD"
BASE COUNT          492 a         1100 c          994 g          518 t
ORIGIN      
        1 ctgctggcgg tgatcggtct gtcggtcttc atttcgcgct ccaccgcctc ggtggtgatg
       61 aagaagaaga gcttctggct gctggcgctg ggcgccgcgt cctcgtcggt gtgcggctat
      121 ggcgtggccg gctggctgcc gctgttcttc atgcgcagct tcggcctgac cctgacccag
      181 gtcagctggt attattccgg catcgctctg gtcggcggca tcgctggcat ctggctgggc
      241 ggcatggtcg ccgacaagct gggcaagcgc tccaagggcg cctatccgtt ggtgccggcc
      301 atcgccttcc tgatctcggc cccctgcttc atcctcgcca tgaactcggg ctggctgatc
      361 gagcaggtca tgcccggcgc cggcggcaac agcttcgccg ccctgaccat ggcctttctg
      421 atcttcctca tcccgaccgg tctgaacctc gtctggctgg ggccgatcac cgcggcggtt
      481 cagcatctgg tgcctgcgcc catgcgcacc acggcctcgg ccctgttcct gctgatcaac
      541 aacctgctcg gcatcgccgt cggcttcttc tacttcggct ggatgagcga cctgctggcc
      601 ccgcgcttcg gcgacgaaag ccttcgctgg gccatctaca ccggcatggg cttctatctg
      661 ctggcctcgg ccctgcttat cggcgcctcg cggacgctga agaaggactg ggtggagtaa
      721 acttcagggc cgaccagttc gcgacgattt cgaccagtcc cggaccggtc gaagccggcg
      781 atttcagcca ttcgcgccac ggctgcttca tgaagtggag ccgcgtggtt gcgcctgcga
      841 aggcgcaacc cctataagcg ccgcaactct tccccagtgt tgcggcggct cctcccatga
      901 acattcacga ataccaggcc aagcaggtcc tgaagggctt tggcgcgccg gtcgccgagg
      961 gcgtcgccat cacctcggtg gatcaggccg aggccgccgc gcgctcgctg cccggtcctc
     1021 tctatgtcgt gaagtcccag atccacgccg gcgggcgcgg caagggcaag ttcaaggaac
     1081 tgggccccga cgccaagggc ggcgtgcgtc tcgccttctc gatcgaggac gtgatcaaga
     1141 acgcgaagga catgctgggc aatacgctgg tgaccgccca gaccggcgac gccggcaagc
     1201 aggtcaaccg cctgtacatc gaggacggcg ccgacatcgc ccgtgagctg tactgctcgc
     1261 tgctggtgga tcgtgaacac ggccgcatcg cctatgtcgc ctcgaccgag ggcggcatgg
     1321 acatcgaggc cgtcgcccat gacacgcccg agaagatcca gaccatcgtc atcgacccgg
     1381 ccaccggcgt gaccgaggcc gacgtcgcga aactgaacgc ggcgttcgag ctgaccggcg
     1441 aagccgccga ggatggcaag acgctgttcc cgctgctcta caaggccttc gtcgacaccg
     1501 acatgtcgat gctggagatc aatccgctga tcgtcatgga gaacggccgc ctgcgcgtgc
     1561 tggacgccaa ggtcagcttc gacggcaacg ccctgttccg ccaccccgag atcaaggaac
     1621 tgcgcgacga aaccgaggaa gacgccaagg agatcgaggc ctcggaatgg gacctcgcct
     1681 atgtcgccct ggacggcaac atcggctgca tggtcaacgg cgccggcctg gccatggcga
     1741 cgatggacat catcaagctg tacggcaaag agcccgccaa cttctgtgac gtcggcggcg
     1801 gcgccggcaa ggagaaggtc gcggcggcgt tcaagatcat caccgccgac cccgccgtgc
     1861 agggcattct ggtcaacatc ttcggcggca tcatgaagtg cgacgtcatc gccgagggcg
     1921 tggtcgcggc cgtgaaggaa gtgggcctga aggtgccgct ggtcgtccgt ctggaaggca
     1981 ccaacgccga actgggcaag aaaatcctca acgagtccgg cctgaccatc caggccgccg
     2041 acgacctcga cgacgccgcg cagaagatcg tcgcggcggt cggctgacat gacccgcgcg
     2101 ccctccctcg ccatactgct gatcgccgcc tgcgcggcga cggcggccgg ggcccagaca
     2161 gcgccgcgag gcgatatgcg gatcacgcgc gtgaccgacc cggtgctggg ctgcaccctg
     2221 atcacgacga gcgaagtttc gcggtcgggc gccgggaacg tcaccctcgg ccgcgcgcgg
     2281 accagtcggg agggcgaaac gaccatcgtg tcgtggagcc gcgtccgggt tgagggccag
     2341 cgcgatcgca agcccgcagt ctgccgcgcc gagcccggcg ccatcacctc cgtgacgtgg
     2401 ggcggcgtgg aactgctcgc ccggccgcag ccgttctgac catgaagtcg ctccgcctcc
     2461 tttccgttct gatcagcgcc ctcgccgcgg cgtcgccgtc gatcgcgcag gacggtcccg
     2521 aggcggcgcc caccgacctg atcgcgaccc tgaacaccgt ctgcgtggcc gcccggggcg
     2581 accgcgcccg cgccgcggcc ctggcggccg aggccggctt ttctccggcg cctgagagca
     2641 tgacgccccg gatgcgcaac agctccgaaa ccgccggctt catgcgcagc agcgccgccg
     2701 acctcactgt catcatgacc ggcacgatga cccgccgcgt tggccgcgac acagtggtga
     2761 tggagttctg cggcgtcagc gcccgtccga cggatcaccg ggccctcgac gcccggctgc
     2821 ttgacctgat gaacttcccg tcggtccgcg gcgcgggcat ggaagcctat gcctggcttc
     2881 agacgccgga agggcgcgtg cccttacgct cacgccgcct gaccgatccg cagttcctcg
     2941 ccatggccga gaccggccag atgcgccttg tggccctgga ccgctcgggt cccggctcga
     3001 ccctgatcta tttcctgccc cgcctcgact gacgcgtcgc gcccgcccac ccttttcgcc
     3061 tcccggatac ccctgacatg tccatcctcg ttgaccgcca cacc
//
LOCUS       DASVVN010000553         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_285804,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000553 DASVVN010000000
VERSION     DASVVN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285804"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF686_08980"
     CDS             complement(<1..223)
                     /locus_tag="VF686_08980"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503921.1"
                     /note="catalyzes the formation of 5-aminolevulinate from
                     succinyl-CoA and glycine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX8661989.1"
                     /translation="MFDYKAAFETAVEQVKGEGRYRVFADLKRVRGQFPKAIRRREDG
                     SEQDVIVWCSNDYLGMGQHPVVLEAMQAEI"
     gene            complement(351..1352)
                     /gene="mmsB"
                     /locus_tag="VF686_08985"
     CDS             complement(351..1352)
                     /gene="mmsB"
                     /locus_tag="VF686_08985"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX8661990.1"
                     /translation="MSEKTGRRRWRRGWRRGRGRANAPENHSGDDAMTLKIAFIGLGN
                     MGGGMAANQAKAGNTVAAFDLSQAALDRAAAAGCSPVGSVAEAVKDADVVITMLPAGP
                     HVLKVYSEQIIGAAPTSALLLDCSTIDVDTARKVAGLAKEAGYAFADAPVSGGTMAAD
                     AGTLAFMVGCDEPDFARIEAALEPMSRATFRAGDHGAGQAAKICNNMILGITMLGTCE
                     AIGLAEKLGLDPVKMFDIVAKSSGQSWSVTTYYPWPGPVPTAPSNRNYDGGFAAAMML
                     KDLKLAQDAAAKSGASTPLGAQTEALFQMFNGLGYGGRDFSAILQMLRGKLDELPKG"
     gene            complement(1349..>2001)
                     /locus_tag="VF686_08990"
     CDS             complement(1349..>2001)
                     /locus_tag="VF686_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8661991.1"
                     /translation="ADPGRTAEDRARDEARHAAETLDFAGVRPGQKIADMMMGGGYFT
                     RLFSAAVGPEGRVTAWQPAEFIAFMDSYGDSLTAADALSNVDGVRWSLGAPEFPADLD
                     LVFTAQNYHDLHLNVAPAGTAANVNAAVFRALKPGGLYVIVDHHAVAGAPLTASDTVH
                     RIDIEAVKREVQAAGFVLDGESDILANPADPRTASVFDPSIRGRTSQFMLRFKKPG"
BASE COUNT          306 a          730 c          644 g          321 t
ORIGIN      
        1 cgatctcggc ctgcatggcc tcgagcacca cgggatgctg gcccatgccc agatagtcgt
       61 tcgagcacca gacgatgacg tcctgctcgc tgccatcctc gcggcggcgg atcgccttcg
      121 ggaactggcc gcgcacgcgc ttcaggtcgg cgaagacgcg atagcgcccc tcgcccttga
      181 cctgctccac ggcggtctcg aatgcggcct tgtagtcgaa caaacgaaca gctctgctgg
      241 aggcgaaaac cttgccgcct gatgcgccga agcggacgat ttgtccatga atcggcacgg
      301 acaaaacgcc gcaaacccct aattgataac ggttctcaga aactcgaccc tcagcccttc
      361 ggcagttcgt ccagcttgcc gcgcagcatc tgcaggatgg ccgagaagtc ccggccgccg
      421 tagccgagcc cgttgaacat ctggaacaac gcctccgtct gcgcgcccaa cggcgtcgac
      481 gcccccgatt tcgccgccgc atcctgcgcc agcttcagat ccttcaacat catggccgcc
      541 gcgaaaccgc catcgtagtt tcggttcgac ggcgcggtcg gcaccggccc cggccacgga
      601 tagtaggtcg tcaccgacca gctctgcccc gacgacttgg ccacgatgtc gaacatcttg
      661 accgggtcca gccccagctt ctcggccagg ccgatcgcct cgcaggtccc cagcatggtg
      721 atgccgagga tcatgttgtt gcagatcttg gccgcctgcc cggccccgtg gtcgccggcg
      781 cggaaggtcg cgcggctcat cggctccagc gcggcctcga tacgggcgaa gtccggctcg
      841 tcgcagccca ccatgaaggc cagcgtcccg gcgtccgccg ccatggtccc gcccgacacc
      901 ggcgcgtcgg cgaacgcgta gcccgcctcc ttcgccagcc cggccacctt gcgcgccgtg
      961 tccacgtcga tggtcgaaca gtccagcagc agggcgctgg tcggcgccgc gccgatgatc
     1021 tgctccgaat agactttcag cacatgcggc ccggcgggca gcatggtgat gaccacatcg
     1081 gcgtccttca ccgcctcggc gaccgacccg acgggcgagc agccggcggc ggcggcgcgg
     1141 tccagcgcgg cctgcgacag gtcgaaggcg gcgacggtgt tgccggcctt cgcctgattg
     1201 gccgccatgc ccccgcccat gttgcccagg ccgatgaagg cgatcttgag ggtcatggcg
     1261 tcgtctcccg aatgattttc gggagcgtta gcccgtcccc tgccccgtcg ccagccccgt
     1321 cgccagcggc gccgcccggt tttttcgctc agcccggctt cttgaagcgc agcatgaact
     1381 ggctggtccg cccgcggatc gacggatcga agacgctggc ggtgcggggg tcggcgggat
     1441 tggcgagaat gtcgctctcg ccgtccagca cgaagccggc cgcctgaacc tcgcgcttga
     1501 ccgcctcgat gtcgattcgg tgcaccgtgt cggaggccgt caggggcgcg ccggcgacgg
     1561 cgtggtggtc gacgatgaca tagaggccgc cgggcttcag ggcgcggaag acggcggcgt
     1621 tgacgttcgc ggccgtcccc gccggggcga cgttcagatg caggtcgtga tagttctggg
     1681 ccgtgaacac caggtccaga tccgccggaa actccggcgc tcccagcgac cagcggaccc
     1741 cgtccacgtt cgacagcgcg tccgccgccg tcaggctgtc gccatagctg tccatgaagg
     1801 cgatgaactc cgccggctgc cacgccgtga cgcgcccctc gggccccacg gccgccgaaa
     1861 acaggcgggt gaaatagccg ccgcccatca tcatgtcggc gattttctgg ccgggccgaa
     1921 ccccggcgaa gtccagggtt tcggccgcgt gccgcgcctc gtcccgggcc cggtcctcgg
     1981 ccgtccggcc cgggtccgcc a
//
LOCUS       DASVVN010000554         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_257529,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000554 DASVVN010000000
VERSION     DASVVN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257529"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF686_08995"
     CDS             <1..215
                     /locus_tag="VF686_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8661992.1"
                     /translation="YLLAFLGMLISGRFQSASIDWGQDFSWFLIGLLGFVGLPMLLFA
                     SSSVIGYFVAPAPKRARRLKDWRLPQ"
     gene            315..1922
                     /gene="xseA"
                     /locus_tag="VF686_09000"
     CDS             315..1922
                     /gene="xseA"
                     /locus_tag="VF686_09000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX8661993.1"
                     /translation="MSDDSYVFEGPADEPPPPARDNNPPQTVSELSFALKRTLEDRFG
                     HVRLRGEISKVNHHASGHVYLTLKDDKAAIDGVIWKGSVRGLGVRPESGLEVIVTGKI
                     TSYPARSSYQIVIETMEAAGAGALLAQLERLKAKLAGEGLFEAGRKKPIPTFPAVVGV
                     ITSPTGAVIRDILHRIAERWPCRVIVWPVVVQGDAACGQVSAAIRGFDAMKPDGPIPR
                     PDILIVARGGGSVEDLWCFNDESLARTVAAASIPLISAVGHETDTTLIDFVSDRRAPT
                     PTGAAEIATPVLADLRYAVADLDRRLARAGGRILEDRRTRLRAAARGLPARPEDVLAL
                     PQQRLDHAGSRLASGLTRNVALHERNLVGVTGRLSPALLDRAMERRRDRLESISARLG
                     SGLTANVKAHEHRLLRALARLSPEPLHRRLDQRAARLESTGARLVALPRRLERDDARL
                     AALSRALASLNPKTPKPGFARVESADGAMIAAAAALREGQAIRLIFADGSKGARIDGE
                     GAPPPARPRPSPAPKPKAPPPGQGDLF"
     gene            1971..2174
                     /locus_tag="VF686_09005"
     CDS             1971..2174
                     /locus_tag="VF686_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX8661994.1"
                     /translation="MARPSSSETATLHYGDGEFAVLKPGRAVICAVSGKPIPLETLRY
                     WSVSKQEAYAGPTEAFQGLGQHP"
     gene            2171..>2653
                     /locus_tag="VF686_09010"
     CDS             2171..>2653
                     /locus_tag="VF686_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEX8661995.1"
                     /translation="MILPRRQLLIGGALAGLMPSAAWARQPVELALTGRFVQGGHAIG
                     RTWPRALIFVDGESLTTASAGGDFIVGFDRDAPGSVQIEARLDGRSAMRTLTIAPGQF
                     PSSRIDGLPPATIEPSDPALLARIQREVALKAEGFASRVDADHFSGGFVWPLESFRVT
                     S"
BASE COUNT          363 a         1074 c          825 g          391 t
ORIGIN      
        1 cctacctgtt agcctttttg gggatgctga tttcgggtcg tttccaaagc gcgtcgatcg
       61 actggggcca agatttttcg tggttcctga tcgggcttct cggctttgtg ggacttccca
      121 tgctcctctt cgcaagtagc agcgtgatcg gctactttgt cgctccggca cccaagcgcg
      181 cgcggcgtct gaaagattgg cgactgccac agtagggcgg acccaacgtc cgcttcccac
      241 ccatagcagc cttcggaaac atctgctccc cgccccccat tcccgccgcc gcctccgccc
      301 gctaccctgc ccccatgagc gacgactcct acgtcttcga gggcccggcc gacgaaccgc
      361 cgccgcccgc gcgggacaac aatcctccgc agacggtctc ggaactgtcg ttcgccctca
      421 agcgcacgct cgaggaccgc ttcggccacg tccggctgcg cggcgagatc tccaaggtga
      481 accaccacgc ctcgggccac gtctatctga ccctcaagga cgacaaggca gccatcgacg
      541 gcgtcatctg gaagggctcc gtccgcggcc tcggcgtccg gcccgagtcc ggactggaag
      601 tcatcgtcac cggcaagatc accagctatc ccgcccgctc ctcctaccag atcgtcatcg
      661 agaccatgga ggccgccggc gccggcgccc tgctggccca gctggaacgg ttgaaggcga
      721 aactggccgg cgagggcctg ttcgaggcgg gccgcaagaa gccgatcccg accttcccgg
      781 ccgtggtcgg cgtcatcacc agcccgaccg gcgcggtcat ccgcgacatc ctgcatcgca
      841 tcgccgaacg ctggccgtgc cgcgttatcg tctggccggt cgtggtccag ggcgacgccg
      901 cctgcggcca ggtctccgcc gccatccgcg gtttcgacgc catgaagccg gacggcccca
      961 tcccgcgccc cgacatcctg atcgtcgccc gtggcggcgg ctcggtcgag gacctgtggt
     1021 gtttcaatga cgagagtctg gcccgcaccg tcgccgccgc ctccatcccc ctcatctcgg
     1081 ccgtggggca cgagaccgac accaccctga tcgacttcgt ctccgaccgc cgggcgccca
     1141 cgccgacggg cgcggccgag atcgccaccc cggtgctggc cgacctgcgc tacgccgtcg
     1201 ccgacctcga ccgccgcctc gcccgcgcgg gcggccgcat tctcgaggat cgccgcaccc
     1261 gcctgcgcgc cgccgcccgg ggcctgcccg ctcgtcccga ggacgtcctg gccctgccgc
     1321 agcagcggct cgaccacgcc ggcagccggc tcgcctcggg tctgacccgg aacgtcgccc
     1381 tgcacgagcg caatctggtc ggcgtcaccg gccgcctgtc cccggccctg ctcgaccgcg
     1441 ccatggagcg tcgccgcgac cggctggaat cgatatccgc ccgcctcggc tccggcctca
     1501 ccgccaatgt gaaggcgcac gagcatcgcc tgctgcgcgc cctcgcccgt ctttcgcccg
     1561 aaccgctgca ccgacgcctc gaccagcggg cagcgcggct cgaatccacc ggagcccgtc
     1621 tggtcgccct gccgcgccgg ctcgaacgcg acgacgcccg cctcgctgcc ctgtcccgcg
     1681 ccctcgcgag cctgaacccc aaaacgccca agcccggctt cgcccgggtc gagagcgcgg
     1741 acggcgccat gatcgccgcc gccgccgccc tgcgtgaggg tcaggccatc cgcctgatct
     1801 tcgccgacgg gtcgaagggc gcccgcatcg acggcgaagg ggcgccaccc cccgcccgcc
     1861 cgaggccttc gcccgcaccg aagcccaagg ccccgccgcc cggccagggc gacctcttct
     1921 gaggcgggaa atccgtggcg cagccggacg cgccgcgcta gcctgacgcg atggccagac
     1981 cttcctcttc agagaccgcg accctccact atggcgacgg cgaattcgcc gtgctgaagc
     2041 cgggccgcgc cgtgatctgc gccgtttccg gcaagcccat cccgctggag accctgcgct
     2101 actggtcggt cagcaagcag gaggcctacg ccggcccgac cgaggccttc cagggtctgg
     2161 gccagcaccc ttgatcctgc cgcgccgcca gctgctgatc ggcggcgcgc tcgccggcct
     2221 gatgccgtcg gcggcatggg cccggcagcc ggtcgaactc gccctgaccg gccgcttcgt
     2281 ccagggcggc cacgccatcg gccggacctg gccccgggcg ctgatcttcg tcgacggcga
     2341 atccctgacc accgcctcgg cgggcggcga cttcatcgtg ggcttcgacc gcgacgcgcc
     2401 gggcagcgtc cagatcgagg cccgcctcga cggccgttcc gccatgcgga ccctgaccat
     2461 cgcgcccgga cagttcccgt caagccggat cgacggcctg ccgcccgcga ccatcgagcc
     2521 gagcgatccc gcccttctgg cccgcatcca gcgcgaggtc gcgctcaagg ccgagggttt
     2581 cgccagccgg gtcgacgccg atcatttcag cggcggcttc gtctggccgc tcgagagctt
     2641 ccgcgtcacc agc
//
LOCUS       DASVVN010000555         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_367262,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000555 DASVVN010000000
VERSION     DASVVN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367262"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VF686_09015"
     CDS             complement(<1..645)
                     /locus_tag="VF686_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX8661996.1"
                     /translation="MKLAIVSAAAIGAALAAGSASAMDGPSAKIEHTVARVVIIVEDR
                     ADIGVEIEQGTSGLPAVQVRRRGDDVIINGGLKGGLFGNNAIRNCQSGPEGARQPGEG
                     ASVEVREVGRVDMANAPLIVIRTPRAVEVAVESGAVFGAVGRGASSIEIGNGGCGDWN
                     VANTEGDLSLSIMGSGDMRAGTSGRLEASIMGSGDIQAGATGRLEANVMGSGDIS"
     gene            complement(797..>1663)
                     /locus_tag="VF686_09020"
     CDS             complement(797..>1663)
                     /locus_tag="VF686_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8661997.1"
                     /translation="KVHPHSLIEQGFASDEALAAVLDRYPAHLFDINLYDYDEAGQVS
                     LRTGARGRLNGEELLAAIQQGRLWVNLREVETGWPELWAAAMEEFRKVQGAYPGLRAV
                     RNAGQLILSSPKARVPYHFDAAGVVLFHMRGRKRIFVYPGDEAHLAEADREQVVSRQT
                     TEELPYSLAFEADAQVMDLEPGQALTWPIYAPHRVENLDRFCVSLSMDFQTWPSRFRN
                     GALYTNAVLRSRGGQPRMTDRMAGPELAARWAASLALKRVGGLKSRIAHFERDFEPEV
                     GAADGAGALQTT"
BASE COUNT          260 a          581 c          546 g          276 t
ORIGIN      
        1 gctgatgtcg ccagatccca tgacattggc ctcgagccgg ccggttgcgc cggcttggat
       61 gtcgcccgag cccatgatgc tggcttccag ccggccggac gttcctgcgc gcatgtcgcc
      121 cgagcccatg atggaaaggg acaggtcccc ctcggtgttg gcgacattcc agtccccaca
      181 gccgccattg ccgatttcga tcgaggaggc tccgcggccg acggcgccga acacggcgcc
      241 gctctcgaca gcgacctcga ccgcgcgggg cgtccgaatg acgatcagcg gcgcgttggc
      301 catgtcgacc cttccaacct cgcgcacctc gaccgaggcg ccttcgcccg gctggcgcgc
      361 cccttcggga ccggactggc agttccggat ggcgttgttg ccgaacaggc cgcctttcag
      421 gccgccgttg atgatgacgt cgtcgccgcg gcggcgaacc tgaaccgcgg ggaggcccga
      481 ggttccctgc tcgatctcga cgccgatatc ggcgcggtct tccacgatga tcacaacgcg
      541 tgcgacggta tgctcgatct tggcggacgg cccgtccatg gccgaggcgg aaccggcggc
      601 gagtgcggcg ccgatggcgg cggcggatac gatggcgagt ttcatgacgg cgttccctct
      661 ccggaatgtg acgggaaggt gcgaccggcc aacagtgaag tcagcttaaa acgaggtcat
      721 gacttcgaat tcatcgaagt cgtcggtgtc gccgggaatt ccgtcgtcgg agtcttcgaa
      781 atccgtcgtc caattctcag gtggtttgca gcgcgcccgc gccgtcggcc gccccgacct
      841 caggttcgaa atcgcgctcg aaatgggcga tccggctctt cagccccccg acccgcttca
      901 gggccagcga ggcggcccag cgggcggcca gttcgggtcc cgccatgcgg tcggtcatcc
      961 gcggctgtcc gccccggctg cgcagcacgg cgttggtata cagggcgccg ttcctgaacc
     1021 gcgacggcca ggtctggaag tccatcgaca gggagacgca gaaccggtcc aggttctcga
     1081 cccggtgcgg cgcatagatc ggccaggtca gggcttggcc gggctcgaga tccatcacct
     1141 gggcgtcggc ctcgaaggcc agcgaatacg gcagttcctc ggtcgtctgg cgcgacacca
     1201 cctgctccct gtccgcctcg gccagatgcg cctcgtcgcc gggatagacg aagatccgct
     1261 tgcggccgcg catgtggaac agcaccacgc ccgccgcgtc gaaatgataa ggcacccgcg
     1321 ccttgggcga agacaggatc agctggccgg cgttgcgcac ggccctcagg cccggatagg
     1381 cgccctgcac cttgcggaac tcttccatcg cggccgccca cagctcgggc cagccggtct
     1441 cgacctcgcg cagattgacc cacagccggc cctgctggat cgccgccagc agctcctcgc
     1501 cgttcagccg tccccgtgcg cccgtgcgca gcgagacctg acccgcttcg tcgtagtcgt
     1561 acagattgat gtcgaacaga tgggccggat agcggtccag caccgccgcc agcgcctcgt
     1621 cggaggcgaa gccctgctcg atcaggctgt gcggatggac ctt
//
LOCUS       DASVVN010000556         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_285877,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000556 DASVVN010000000
VERSION     DASVVN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285877"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VF686_09025"
     CDS             <1..1394
                     /locus_tag="VF686_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEX8661998.1"
                     /translation="PAAGAFAGAVQAQTAPVAPPGGPPVFPAPNTAPPIHQVGDTPPP
                     WPTWDGPVPADWVDPATGHRVVRLSGDEGGNKLYFYRNSFTPQGDVLVFSSSEGIIKV
                     ELNNGFRRSVLVPDANADLMVTGRKTRTAYYTVTDAGEGQPSDRGRTLHAVDIDTGAT
                     RRIARLDQGSFSSINADETLCVGTVAYGDAPLQPNAADPRNRRAGQAEYAALGPDGRP
                     LNFAKAKGVRMLQRWAARVPMELFVVDLRTGERRVLHSATDWLNHPQFSPTDPDRIIF
                     SHEGPWHRVDRMWTIRTDGTDLRKLHQRTMNFEIFGHEWFSPDGKAVMYDLQTPRGQV
                     YWVASVDLETGKRIYRRVEQNSWSVHYNTAPDGSLFAGDGGDADMVAHAPDGKWLVLL
                     RPEPLIDGDPDSDQEAMISVEYLRTERLVDMSRHDYRLEPNVTFTPDGRWVLFVSNMH
                     GRNHVYAAEVTRA"
     gene            1539..>2800
                     /locus_tag="VF686_09030"
     CDS             1539..>2800
                     /locus_tag="VF686_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8661999.1"
                     /translation="MSRSSQVRVTLASSASAIAILAVSFAPQMAAAQTAPADPVAQDE
                     ATEIDEVVVTGFRSSLQRALNIKRQEAGAVDAILAEDIADFPDQNLAEAIQRLPGVTI
                     DRVNGQGTTISVRGLGSDFTRTRINGLEAQAASGGNRNRSFDFSMFASELFNSIRVRK
                     TQSAEIEEGSLGATVDLQTGRPLDFGGGGFNSALSAQASYNDLSKTTIPRLAGLLSWS
                     NENQTFGALVSVAYSERAPVLGSFNTTRWQRGDAANINPLTGSPYGRGQNFGGCIPCT
                     TTAQRTEVLNAFYPRIPRYTLGETFEDRLGMTGALQWRPSDRTEVDFDMLWSRFNSET
                     ESPNIEAWSFSRAAVNQVVVRDYEIDTSRNAFSYGVFDNMIVAAENGFSRNESNFYQA
                     SINARHDFSDRLHGTLKLGANRSEARTPL"
BASE COUNT          505 a         1003 c          870 g          422 t
ORIGIN      
        1 cgccagccgc cggggccttc gcaggcgccg tacaggccca gaccgctccg gtcgcgccgc
       61 cgggcgggcc tccggtcttc ccggcgccca atacggctcc gccgatccat caggtcggcg
      121 acacaccgcc gccctggccg acctgggacg gccctgtgcc cgcagactgg gtcgatcccg
      181 ccaccggcca ccgcgtcgtc cgactgtccg gcgacgaggg cggcaacaag ctctatttct
      241 accgcaacag cttcacgccc cagggcgacg tgctggtctt ctcctcgtcc gaggggatca
      301 tcaaggtcga gctgaacaac ggtttccgcc gctcggtgct ggtgcccgac gcgaacgccg
      361 acctgatggt cacgggccgc aagacccgaa ccgcctacta caccgtcacc gatgcgggcg
      421 agggccagcc gagcgaccgg ggccgcaccc tccatgcggt cgacatcgac acaggcgcga
      481 cgcgccgtat cgcgcggctg gaccagggct cgttcagctc gatcaacgcc gacgagaccc
      541 tgtgcgtcgg caccgtggcc tacggcgatg cgccgctgca gccgaacgcg gccgaccccc
      601 gcaaccgtcg cgccggccag gccgaatacg ccgccctcgg ccccgacggc cgaccgctga
      661 atttcgccaa ggccaagggc gtgcggatgc tccagcgctg ggcggctcgc gtgccgatgg
      721 agctgttcgt cgtcgacctg cgcacgggcg agcggagggt gcttcactcg gccacggact
      781 ggctgaacca cccgcagttc tctcccaccg acccggaccg gatcatcttc tcgcacgagg
      841 ggccgtggca ccgggtggac cggatgtgga ccatccgcac cgacggaacg gatctgcgca
      901 agcttcacca gcgcacgatg aatttcgaga tcttcggcca cgagtggttc agtcccgacg
      961 gcaaggcggt gatgtacgac ctgcagaccc cgaggggtca ggtctattgg gtcgcctcgg
     1021 tcgatctgga aacgggaaag cggatctacc ggcgggtcga gcagaacagc tggtccgtcc
     1081 actacaacac ggcgccggac ggaagcctct tcgccggcga tggcggggac gcggacatgg
     1141 tcgcccacgc ccccgacgga aaatggctgg tcctgttgcg gcccgaaccg ctcatcgacg
     1201 gcgatcccga cagcgaccag gaagcgatga tctcggtgga atatctgcgc accgagcggc
     1261 tcgtggatat gagccggcac gactaccgac tcgagccgaa cgtgaccttc acgccggacg
     1321 gccggtgggt gctgtttgtc tcgaacatgc acggtcgcaa tcacgtttat gcggccgaag
     1381 tcacccgcgc ctgacggcgt tcagagccgt ttcgctgcca tattgcataa agcgagaagc
     1441 tgttgcacaa agtgggacgg gttgtgatcc caaggaccgc aacaccatat caacaaatga
     1501 caccggtgcc atgagcgccg ggcggggagg aagacaatat gagccggtcc agccaggtca
     1561 gagtcacgct cgcgtccagc gcatccgcta tcgcgatact cgccgtctcg ttcgcgccac
     1621 agatggccgc cgcccagacg gcgccggccg atccggtcgc gcaggacgag gcgaccgaga
     1681 tcgatgaagt cgtcgtcacc ggcttccgct ccagcctgca gcgcgccctg aacatcaagc
     1741 gccaggaagc gggcgccgtg gacgccatcc tggccgagga catcgccgac ttcccggacc
     1801 agaacctggc ggaagcgatc cagcgcctgc cgggcgtcac catcgaccgc gtcaacggtc
     1861 agggcacgac catctcggtg cgtggccttg gctccgactt cacccgcacc cgcatcaacg
     1921 gcctggaggc ccaggcggcg tccggcggca accgcaaccg cagcttcgac ttctcgatgt
     1981 tcgcctcgga gctgttcaac agcatcaggg tccgcaagac ccagtcggcc gagatcgagg
     2041 aaggctcgct cggcgccacg gtcgatctgc agaccggtcg cccgcttgac ttcggcggcg
     2101 gcgggttcaa ctccgccctg tcggcgcaag cctcgtacaa cgacctgtcc aagacgacaa
     2161 ttccgcgcct cgctggcctg ctgagctggt ccaacgagaa ccagaccttc ggcgccctgg
     2221 tgtcggtcgc ctactccgag cgcgcgccgg tcctcggcag cttcaatacg acgcgctggc
     2281 agcgcggcga tgcggcgaac attaacccgc tcaccggctc gccctacggc cgcggtcaga
     2341 atttcggcgg ctgcattccc tgcaccacca ctgcccaacg caccgaggtg ctgaacgcct
     2401 tctatccgcg catcccccgc tacacactcg gcgaaacctt cgaggatcgt ctgggcatga
     2461 ccggggccct gcaatggcgc cccagcgacc gcaccgaagt ggacttcgac atgctgtggt
     2521 cgcgcttcaa ctcggaaacc gaaagcccca acatcgaggc ctggtcgttc agccgcgccg
     2581 ccgtgaacca ggtcgtcgtt cgggactatg agatcgatac gtcgcgcaac gccttctcct
     2641 acggcgtctt cgacaacatg atcgtcgcgg ccgagaacgg cttctcgcga aacgaaagca
     2701 acttctacca ggcgtcgatc aacgcccgtc acgacttcag cgatcgcctg catggcaccc
     2761 tgaagctggg cgccaaccgc tcggaagccc gcacgccgct
//
LOCUS       DASVVN010000557         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_99268, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000557 DASVVN010000000
VERSION     DASVVN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99268"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VF686_09035"
     CDS             <1..1061
                     /locus_tag="VF686_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX8662000.1"
                     /translation="GGQACVQVFFYRGGQNWGGRAYFPRVDRTDTDPEILAAFLGQFY
                     EDKPIPRLILSNVRPHELELLEEAFSMKAERKVEIANPRRGEKLALVDHALTNAREAL
                     GRKMAEGSAQGKILDELCEAFGLDTRPERIEIYDNAHIQGTNAVGGMVVAGPEGFQKS
                     QYRKFNIRGEDLTPGDDYGMMREVMRRRFSRLVKDEDAGEEVVRPDLLLIDGGAGQLA
                     EVLAVLADLGLDDITAVGVAKGPDRDAGLERFFMPGKPPFMLPLKSPALYYIQRLRDE
                     AHRFANGAHRTRRSMDIKKNPLDEIEGIGPGRKKALLHAFGSAKGVSRASVVDLVKVE
                     GINQPMAERIHAFFHRTA"
     gene            1133..1444
                     /locus_tag="VF686_09040"
     CDS             1133..1444
                     /locus_tag="VF686_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662001.1"
                     /translation="MIAFVFATAMLLDPTPKPAQLQKAIQPVAEAPRESSSSVVLSNV
                     AVTLECTARASGRVENCKVLGETHPGLGFGEAAIVLMRDAEVAPGPRDVLFARTIEFM
                     P"
     gene            1496..2122
                     /locus_tag="VF686_09045"
     CDS             1496..2122
                     /locus_tag="VF686_09045"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8662002.1"
                     /translation="MTDESRDALLSGYRRFREGHWAEAKAEYEALAAKGQRPHTLVVA
                     CSDSRADPALIFDAAPGQLFVVRNVANLVPPYEPDGHLHGVSAALEFGVKVLNVSRIM
                     VMGHAFCGGVNAMIYGAPADCQDFVAPWVAQGSETVRLVCEEGPPEELMQRAEEAVVR
                     LSLDNLRTFPWIAEREAAGELKLHGAHFSIAEGILYVLDEAEGDFRPA"
BASE COUNT          393 a          818 c          747 g          367 t
ORIGIN      
        1 agggcggtca ggcctgcgtc caggtcttct tctaccgcgg cggccagaac tggggcggcc
       61 gcgcctattt cccgcgtgtc gaccggaccg acacagaccc cgaaatcctc gccgccttcc
      121 tcggccagtt ctacgaggac aagccgatcc cgcgcctgat cctgtccaac gtccgaccgc
      181 acgagctgga actgctggaa gaggccttct ccatgaaggc tgaacgcaag gtcgagatcg
      241 ccaacccccg gcgcggcgaa aaactggcgc tcgtcgacca cgccctgacc aacgcccgcg
      301 aggcgctggg ccgcaagatg gccgagggct cggcccaggg caaaatcctc gacgagctgt
      361 gcgaggcctt cggtctcgac acccgccccg agcggatcga gatctacgac aacgcccaca
      421 tccagggcac caacgccgtc ggcggcatgg tcgtggccgg ccccgagggc ttccagaaat
      481 cgcagtaccg caagttcaac atccgcggcg aggacctgac ccccggcgac gactacggca
      541 tgatgcgcga ggtcatgcgc cgccgcttct cgcgtctggt caaggacgag gacgcgggcg
      601 aggaggtggt ccgcccggac ctgctgctga tcgacggcgg cgcgggtcag ctggccgagg
      661 ttctggccgt gctggccgat ctgggtctgg acgacatcac cgccgtcggc gtggccaagg
      721 gccccgaccg cgacgcggga ctcgagcggt tcttcatgcc gggcaagccg cccttcatgc
      781 tgccgctgaa atcgccggcc ctctactaca tccagcgttt gcgcgacgag gcgcaccgct
      841 tcgccaacgg cgcccaccgc acccgccgct cgatggacat caagaaaaac ccgctcgacg
      901 agatcgaggg catcggtccc ggccgcaaga aggccctgtt gcacgccttc ggctccgcca
      961 agggcgtcag ccgcgcctcg gtggtcgatc tggtcaaggt cgagggcatc aaccagccga
     1021 tggccgagcg gatccacgcc ttcttccacc gaacggcctg acggcggttc caagcggagc
     1081 cggaggaacg gaaagggccc ccgattatta acgggacaga gagggtcctg taatgatcgc
     1141 gttcgtcttc gccaccgcca tgcttctgga cccgacgccc aaaccggctc agctccagaa
     1201 ggcgatccag ccggtcgcgg aagccccgcg ggaaagctcg tcgagcgtgg tcctgtccaa
     1261 cgtcgcggtc acgctcgaat gcaccgcccg ggccagcggt cgcgtcgaga actgcaaggt
     1321 gctgggggaa acccaccctg gcctcggttt cggcgaggcg gcgatcgtcc tcatgcgcga
     1381 cgccgaggtc gcgccgggac cgcgagacgt tctgttcgcc cgcaccatcg agttcatgcc
     1441 gtagtcacag gtcgcccctc tcccccttgg agcgaggatt gcctataccg gcgccatgac
     1501 cgacgaatcc cgcgacgccc tgctctccgg ttaccgccgc tttcgtgaag gccactgggc
     1561 cgaggccaag gccgagtacg aggcgctcgc cgccaagggt cagcggcccc acaccctggt
     1621 cgtcgcctgc tccgacagcc gcgccgaccc cgccctgatc ttcgacgccg cccccggcca
     1681 gctgttcgtg gtccgcaacg tcgccaatct ggtccccccc tatgagcccg acggccacct
     1741 gcacggcgtc tcggcggcgc tggaattcgg ggtcaaggtg ctgaacgtct cccgcatcat
     1801 ggtgatgggc cacgccttct gcggcggggt gaacgccatg atctacggcg ccccggccga
     1861 ctgtcaggat ttcgtcgcgc cctgggtggc gcagggctcg gaaaccgtcc gcctcgtctg
     1921 cgaggaaggc ccgcccgaag agctcatgca gagggccgag gaggcggtgg tccgcctgtc
     1981 cctcgacaat ctgcgcacct tcccgtggat cgccgagcgc gaggcggccg gtgagctcaa
     2041 gctccacggc gcgcatttct cgattgccga gggcatttta tacgtgctcg acgaggcgga
     2101 aggtgacttc cgccccgcct agccggcatg ctcttttagc tagaacgtcc tagcagcgaa
     2161 cctggtagtt gtagtgcggc tgaccgttct ggtcgacgta atagcaatag ccgcgggtat
     2221 cgcgataata ttgctggtta ttgatccgag cgccgagaac gcctccgagg accgcaccgg
     2281 cgagcgcgcc cgtggtgctg ctcacaccgg ggatgatgcg tccgg
//
LOCUS       DASVVN010000558         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_74722, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000558 DASVVN010000000
VERSION     DASVVN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74722"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF686_09050"
     CDS             complement(<1..388)
                     /locus_tag="VF686_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8662003.1"
                     /translation="MTTTDTAETSIRPGYRYYVLAILILVYMLNFLDRQIIGILAAPL
                     KAHFQLSDQQFGLLGGIAFASVYSTLAIPIAWLADRFSRVWIMTAALTVWSGFTALCG
                     MAGSFGQLFLCRMGVGVGEAGGVAPSY"
     gene            complement(513..2729)
                     /locus_tag="VF686_09055"
     CDS             complement(513..2729)
                     /locus_tag="VF686_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8662004.1"
                     /translation="MNRYVKTALLAGAAWSALSSFAMAQDAVPQDGATSVDDVIVTAR
                     RRAEVLQDVPVAVTAISAETLENRGTADITELARTTPSLTLNSARGSNSTLISFIRGV
                     GQQDPLWGFDPGVGLYIDDVYVARPQAAVLDIFDVERIEVLRGPQGTLYGRNTIGGAI
                     KYVTRRIGDEPLISLKGAYGSYDQFDAIGSISGPVSDTVGFSAGVARYLRDGYGTNRN
                     TGTEHYNKDVTAARASVEWTPDDSLFFRLAGDYVKDSSNARHGHREFAPIPANEYDTF
                     AGAGDRNEVETRGISLTGEWQANDWLTLKSVTAYRDGSTRGNIDFDNQAAPILDIPAE
                     YDDDQFSQEFQALMEGDRWSGVAGLFYLDATASGAFDTVVGLANLTTLTAGSVDTKSW
                     SAYGDFSYDLTDQLSVSVGGRFTHDEKTGTVFRQQYLGIRSPYFGNAAAVPLGAPRTN
                     YTDTKEFEEFTPRVSVTYEITPDLTTYASWGRGFKSGGFDMRGDAVLYPATVNGYEPE
                     TVETIELGLKGRLFDNFTFATAIFDSAYEDQQITTQYPAGATVASVVDNVGSSSIRGW
                     EFEGQFRLGDNLVLNGMVSYIDAQFDEFLAYIPTGPLNSSCPALVGCFVDVSDLRDFQ
                     NTPEWTGAISATYTHNFSDGSYLNFTPSAAYRGAYQQFETAAPLLDQESYWMYDASAV
                     WTSASERWTVALNGKNLTDERYKTGGYNFPGALTGNSIIAFYGAPRTYTFSVGVKF"
     gene            2906..>3110
                     /locus_tag="VF686_09060"
     CDS             2906..>3110
                     /locus_tag="VF686_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8662005.1"
                     /translation="MTKLDAPPARRGRPPKAKAAGSADTRDLILDASEDLFSKHGFYG
                     VTIREVAREAGVDTALVHYYFGAK"
BASE COUNT          524 a          934 c         1106 g          546 t
ORIGIN      
        1 aataggacgg cgccacgccg cccgcctcgc ctacgcccac gcccatccgg cacaggaaca
       61 gctggccgaa cgatccggcc atgccgcaca gggcggtgaa gccgctccag acggtcaggg
      121 ccgccgtcat gatccagacg cggctgaacc ggtcggccag ccaggcgatg ggaatggcga
      181 gcgttgaata gaccgaggcg aaggcgatgc cgcccaacag gccgaactgc tggtcgctga
      241 gctggaaatg ggccttcagg ggcgcggcca ggatgccgat gatctgccgg tccaggaagt
      301 tcagcatgta gaccaggatc aggatggcca gcacatagta gcgatagccg ggccggatcg
      361 aggtctctgc ggtgtccgtg gtcgtcatga tcttcccccg acggtcttct tattggctgg
      421 gacggtagcg gcgcttttgg acagcgccaa agaaaaaggg cggcggctca ggagaaccgc
      481 cgcccgtgat cagatgcgat caggcttcag gatcagaact tgacgcccac cgagaaggtg
      541 taggtccgcg gcgcgccgta gaaggcgatg atcgagttgc cggtcagggc gcccgggaag
      601 ttatagccgc ccgtcttgta gcgctcgtcg gtcaggtttt tgccgttcag ggccaccgtc
      661 cagcgttcgc tggccgaggt ccagaccgcg ctggcgtcgt acatccagta agattcctga
      721 tccagcagcg gggccgccgt ctcgaactgc tgataggcgc cgcgataggc cgccgacggg
      781 gtgaagttca ggtacgaacc gtcggagaag ttatgggtgt aggtggcgct gatcgcgccg
      841 gtccattccg gcgtgttctg gaagtcgcgc aggtccgaga cgtcgacgaa gcagccgacc
      901 agggccgggc aggacgagtt cagcgggccc gtcgggatgt aggccaggaa ctcgtcgaac
      961 tgggcgtcga tgtagctcac catgccgttc aggacgaggt tgtcgcccag acggaactgg
     1021 ccttcgaatt cccagccgcg gatcgaggac gagccgacgt tgtcgaccac cgaggccacg
     1081 gtggcgccgg ccggatactg ggtcgtgatc tgctggtctt cgtaggccga gtcgaaaatg
     1141 gcggtcgcga aggtgaagtt gtcgaacagg cggcccttca ggcccagttc gatggtctcg
     1201 accgtctcag gctcatagcc gttgacggtg gcgggataga gcaccgcgtc gccgcgcatg
     1261 tcgaacccgc ccgacttgaa gccgcggccc cacgacgcat aggtcgtgag atcgggggtg
     1321 atctcatagg tgacgctgac gcgcggggtg aactcctcga actccttggt gtccgtgtag
     1381 ttggtgcgcg gggcgcccag cggcacggcg gcggcgttgc cgaaataggg gctgcggatg
     1441 ccgaggtact gctgacggaa gaccgtgccc gtcttctcgt cgtgggtgaa gcggccgccg
     1501 accgaaaccg acagctggtc ggtcagatcg taggagaagt cgccgtaggc cgaccagctc
     1561 ttggtgtcga cgctgcccgc ggtcagggtg gtcaggttgg ccaggccgac gacggtgtcg
     1621 aaggcgccgg aagcggtggc gtcgaggtag aacaggccgg cgacgccgga ccagcggtcg
     1681 ccttccatca gcgcctggaa ttcctggctg aactgatcgt cgtcatactc ggccgggatg
     1741 tccaggatcg gagcggcctg gttgtcgaag tcgatgttgc cgcgcgtcga gccgtcacga
     1801 taggcggtga ccgacttgag cgtcagccag tcgttggcct gccattcgcc ggtcagcgag
     1861 atgccgcgcg tttcgacctc gttcctgtcg ccggcgccgg cgaaggtgtc gtattcgttg
     1921 gccgggatcg gcgcgaactc gcggtggccg tggcgggcgt tcgagctgtc cttcacatag
     1981 tcgccggcca gacggaagaa gaggctgtca tccggcgtcc attccacgct ggcgcgggcg
     2041 gccgtgacgt ccttgttgta gtgttcggtt ccggtgttgc ggttcgtgcc gtagccgtcg
     2101 cgcaggtagc gggcgacgcc agccgagaag ccgacggtgt cgctgacggg accggagatg
     2161 ctgccgatgg cgtcgaactg gtcgtagctg ccgtaggcgc ccttcaggga gatcaggggc
     2221 tcgtcgccga tgcgacgcgt cacgtatttg atggcgccgc cgatggtgtt gcggccgtac
     2281 agcgtgcctt gcgggccgcg cagcacttcg atgcgctcga cgtcgaagat gtccagaacg
     2341 gcggcttggg gacgggcgac gtagacgtcg tcgatgtaca ggcccacgcc cgggtcgaag
     2401 ccccacagcg gatcctgctg gccgacgccg cggatgaagg agatcagggt cgagttcgag
     2461 ccgcgcgccg agttcagcgt caggctgggc gtcgtgcggg ccagctcggt gatgtcggcc
     2521 gtgccgcggt tctccagcgt ttcggcgctg atggccgtga ccgcgaccgg cacatcctgc
     2581 aggacttcag cacggcggcg ggcggtgacg atgacgtcgt cgaccgaggt cgccccgtcc
     2641 tggggaacgg cgtcctgcgc catggcgaag gacgaaagcg cgctccaggc ggcgcctgcc
     2701 agcaaggcag tcttgacgta gcgattcatg attagcccca atcctgtgat ttccagcgag
     2761 acgcttttat tcagcgttca atgattttcg cggaacgtaa tcgttttcag ccgcctgtca
     2821 aacggcgcag cttacccggc gtcagccgcg tcccgacctg tggcgccgtt gcacaggcgg
     2881 gcgaatgggg cctagcttca gcgttatgac caagctcgat gccccgcctg ccagacgtgg
     2941 acggcccccc aaggccaagg ccgccggttc ggccgatacc cgcgacctga tcctggacgc
     3001 gtccgaggac ctgttctcca aacatggctt ctatggcgtg accatccggg aggtggcgcg
     3061 agaggcgggg gtcgatacgg cgctggtgca ctattacttc ggggccaagc
//
LOCUS       DASVVN010000559         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_286223,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000559 DASVVN010000000
VERSION     DASVVN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286223"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2323
                     /gene="infB"
                     /locus_tag="VF686_09065"
     CDS             <1..2323
                     /gene="infB"
                     /locus_tag="VF686_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX8662006.1"
                     /translation="PVVNFGQRVPKAPSPSRAPAGPAFGGRSAREEAMGGGERAYSDR
                     PAPARPTGAAGRPAEPVRYSALTPRPAPGAARPGGARPGPGGRPTPNAPPAQAEVARP
                     SRAPGGGFARPLKPEDDRDKRFSDAGKAVSRTRGEPKRREGRLTIQSVAGDGDTAERM
                     RSLASVRRAREREKEKRKGGSTDAPNRPREVVIPDVITVQELSNRMAVRGVDIIKFLM
                     KQGLMMKINDVIDTDTAELVADEFGMTVRRVSESDVEAGFLSDAIDDEAVTPRAPVVA
                     IMGHVDHGKTSLLDALRTTDVASGEAGGITQHIGAYQVRLESGDRVTFLDTPGHAAFS
                     AMRARGANVTDIVVLVVAADDGVMPQTIEAIQHAKAANAPIIVAVNKVDKPDANPLKV
                     VNELLQYEVIAESLGGDTQIIEVSAKERTNLNGLIDAILLQAEVMDLKADPERSSEGV
                     VIEAKLDKGRGPVATVLVKRGTLKRGDIVVAGSAWGKVRALLNERNEQLTEAGPSVPV
                     EILGLDEAPSPGDPFAVVENEARARELTEYRARIRREKATGGINQVSLVDMMSKLQSK
                     KVSELPVVIKADVQGSAEAIVGSLDKMGNEEVRARTVMSGAGGITESDVQLAKSAGAP
                     ILGFNVRASKQARDLAEREGVEIRYYSIIYDLLDDMKGVLSGMLAPLQRETFLGNAAV
                     LQVFDISKTGKIAGCRVSEGVVRKGAKVRIIRDDVVVLELGTLTTLKRFKDEVNEVPS
                     GQECGMHFQGFQDIKAGDYIECFTVEEIKRTLD"
     gene            2405..2935
                     /locus_tag="VF686_09070"
     CDS             2405..2935
                     /locus_tag="VF686_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662007.1"
                     /translation="MRRFLIAVLTLSALTPVVWAGPAAAETRYLGYNASDRVTQALTR
                     GITLEAERGLFGAISVRRIISTSNRGQADIRRGGPDQVRRALPAGSTETAIYSITPEG
                     GGRALGRALCPGSDETWMVLGRVRLARPLTVHAVGRWSDGTYRHCVQLSYDWRGEWAF
                     PPAGGAADDTNAPVAR"
     gene            2975..3646
                     /locus_tag="VF686_09075"
     CDS             2975..3646
                     /locus_tag="VF686_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662008.1"
                     /translation="MSAAALPRQTGLKRLAGALAFVCLVLFLLAIENRYAIEAWRALR
                     DEAVPTAGRVAEWAGRLPTGAADGLSGLKKAVTKNPTTMAADPGDVMLVGEFGAADES
                     TRAVTGGVAFVGAVVRFDTGETFRTQPLRIAAGREVFIGRETFARRLNAPADAQIELR
                     RIVPQAGARSVAASVLCGGAAPGGMALLHRQDRVDVMVFRAGTFIGPDAPPSALCGVW
                     SFRKR"
BASE COUNT          584 a         1276 c         1257 g          538 t
ORIGIN      
        1 cccggtggtg aatttcggcc agcgcgtacc caaggccccc agcccctcgc gcgcgccggc
       61 cggccccgcc ttcggcggcc gttcggcccg tgaagaagcc atgggcggcg gcgaacgcgc
      121 ctattcggat cgtccggctc ccgctcgtcc gacgggcgcc gccggtcgtc cggccgagcc
      181 ggttcgctat tcggccctga ctccgcgtcc cgcgcccggc gccgcccgtc ccggcggtgc
      241 gcgccccggc cccggcggtc gcccgacccc gaacgcgcct ccggcccagg ccgaggtcgc
      301 ccgtccgagc cgcgcgccgg gcggcggctt cgcccgcccg ctgaagcccg aggacgatcg
      361 cgacaagcgt ttctccgacg caggcaaggc tgtcagccgc acccgcggcg agcccaagcg
      421 ccgtgaaggc cgcctgacca tccagtccgt cgccggcgac ggcgacacgg ccgagcggat
      481 gcgctcgctg gcctcggtcc gccgcgcccg cgaacgcgag aaggaaaagc gcaagggtgg
      541 atccacggat gcgccgaacc gtccgcgcga agtcgtcatc cctgacgtca tcaccgtgca
      601 ggaactgtcc aaccggatgg ccgtgcgcgg cgtcgacatc atcaaattcc tgatgaagca
      661 gggcctgatg atgaagatca acgacgtcat cgacaccgac accgcggaac tggtggccga
      721 cgagttcggc atgaccgtcc gtcgcgtttc ggaatccgac gtcgaggccg gcttcctgtc
      781 cgatgcgatc gacgacgagg cggtcacgcc ccgtgcgccg gtcgtggcca tcatgggtca
      841 cgtcgaccac ggcaagacct ccctgctgga cgccctgcgc accaccgacg tcgcctcggg
      901 cgaggccggg ggcatcaccc agcacatcgg cgcctatcag gtgcgtctgg aaagcggcga
      961 ccgcgtcacc ttcctcgaca cccccggcca cgccgccttc tcggcgatgc gtgcgcgcgg
     1021 cgccaatgtg acggacatcg tggttctggt cgtggccgcc gacgacggcg tcatgcccca
     1081 gaccatcgag gccatccagc acgccaaggc cgccaacgcc ccgatcatcg tggcggtgaa
     1141 caaggtcgac aagccggacg ccaatccgct gaaggtcgtc aacgaactgc tgcagtacga
     1201 agtcatcgcc gagagcctgg gcggcgacac ccagatcatc gaggtgtcgg ccaaggagcg
     1261 gaccaacctc aacggtctga tcgacgccat cctgctgcag gccgaggtca tggacctgaa
     1321 ggccgatccg gaacgatcct cggaaggcgt ggtcatcgag gccaagctgg acaagggccg
     1381 cggcccggtc gccaccgttc tggtcaagcg cggcacgctg aagcgcggcg acatcgtcgt
     1441 ggccggttcg gcctggggca aggttcgcgc cctgctgaac gagcgcaacg agcaactgac
     1501 cgaagccggt ccgtccgtgc cggtcgagat tctgggtctg gacgaggcgc cgagccccgg
     1561 cgatccgttc gccgtggtcg agaacgaggc ccgcgcccgc gagctgaccg aataccgcgc
     1621 ccgcatcagg cgcgagaagg cgaccggcgg catcaaccag gtctcgctgg tcgacatgat
     1681 gtcgaagctg cagtccaaga aggtctcgga actgccggtg gtcatcaagg ccgacgtgca
     1741 gggttcggcc gaagccatcg tcggctcgct ggacaagatg ggcaacgagg aggtccgcgc
     1801 gcgcaccgtc atgtcgggcg ctggcggcat caccgagagc gacgtccagc tggccaagtc
     1861 ggccggtgcg ccgatcctgg gcttcaatgt ccgcgcctcg aaacaggccc gcgacctggc
     1921 cgagcgcgaa ggcgtcgaga tccgctacta ctcgatcatc tacgacctgc tggacgacat
     1981 gaagggcgtg ctgtccggca tgctggcgcc gctgcaacgg gaaaccttcc tgggcaacgc
     2041 cgcggtgctg caggtcttcg acatctccaa gaccggcaag atcgccggtt gccgggtgtc
     2101 cgagggcgtg gtccgcaagg gggccaaggt ccggatcatc cgcgacgacg tcgtggttct
     2161 ggagctgggc accctcacga cgctcaagcg cttcaaggac gaggtcaacg aggtcccgtc
     2221 gggccaggag tgcggcatgc atttccaggg cttccaggac atcaaggccg gcgactacat
     2281 cgagtgcttc acggtcgaag agatcaagcg cacgctggac tagtccgcgc cgcaacgatg
     2341 agaccacagg gaaggcggcg cggaagcgcc gccttttcgc cttacccgtc agagatcaaa
     2401 ccgcatgcgc cgtttcctga tcgccgtcct gaccctgtcc gccctcaccc cggttgtctg
     2461 ggcgggcccc gccgcggcgg agacgcgcta tctcggctac aacgcctcgg accgggtgac
     2521 gcaggcgctg acgcgcggca tcacacttga ggccgagcgc ggcctgttcg gcgctatcag
     2581 cgtgcgccgg atcatttcca cctcgaaccg cggccaggcg gatatccggc gcggcggtcc
     2641 cgatcaggtg cggcgcgccc tgccggccgg gtcgacggaa acggcgatct attccatcac
     2701 gcccgaaggc ggcggccggg cgctgggccg ggccctctgc cccgggtcgg acgagacctg
     2761 gatggtgctg ggccgggtgc ggctggcgcg gccgctgacg gtccatgcgg tcgggcgctg
     2821 gtcggacgga acgtatcgtc actgcgttca gctgtcctac gactggcgcg gggaatgggc
     2881 gttcccgcct gccggcggag ccgccgacga caccaacgcg cctgtcgcgc gataggcgtt
     2941 cacctccccg caccggctcg ctaaggttgg gtcgatgtcc gccgccgcac tcccccgaca
     3001 gaccggattg aaacgcctcg ccggtgcgct ggcgttcgtg tgcctggtgc tgttcctgct
     3061 ggccatcgag aaccggtatg cgatcgaagc ctggcgggcg ctgcgagatg aggccgtgcc
     3121 cacggccggc cgcgtcgccg agtgggctgg tcggttgccg acgggcgccg ccgatggact
     3181 gtcgggtctg aagaaggccg taacgaagaa cccgacgacc atggctgcag accccggcga
     3241 tgtgatgctg gtcggggagt tcggggcggc ggacgaaagc acgcgcgcgg tgaccggagg
     3301 cgtcgccttc gtcggcgccg tggtgcggtt cgacacgggt gagaccttcc gcacccagcc
     3361 tttgcggatc gccgcgggac gtgaggtctt catcgggcgc gagacgttcg ccagacggct
     3421 gaacgctcct gccgacgcgc agatcgaact acgacgtatc gttccacaag ccggggcccg
     3481 ctcggtcgcg gcctccgtcc tgtgcggcgg cgccgctccg ggagggatgg ccctgctgca
     3541 tcggcaggat cgggtcgatg tcatggtgtt ccgcgccgga acctttattg gccccgatgc
     3601 gccgccttcc gccctgtgcg gcgtctggag cttccgcaaa cgatgaaaca ccgcc
//
LOCUS       DASVVN010000560         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_521363,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000560 DASVVN010000000
VERSION     DASVVN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521363"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..970)
                     /locus_tag="VF686_09080"
     CDS             complement(62..970)
                     /locus_tag="VF686_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8662009.1"
                     /translation="MRRLTAVLLIAASAAPLLVTPGLAGAQTPPATIGERYVPAPWWM
                     RDPVIASVGYVRTEVAANRASFGATFQSIDRSAAEASRKAADQVRALSQALAAYGVDK
                     VRVETSLTTQPLYDQYRDENGVLRDNARGDQIERYQANANVSVTVRDGSVLERVYATV
                     LAAQPTSTSPVSFHLEPDNAVKTWLQGEAVKDAARRAREAASNAGAALGGVRVIDPTG
                     RACETDVLAGWPSYASGSGLATDVDAVTVTGSRMERQFAPSAPPPPPPPPGAAPSEAQ
                     IEAARLALQPPLRELTDRACVVYGLN"
     gene            1193..1747
                     /locus_tag="VF686_09085"
     CDS             1193..1747
                     /locus_tag="VF686_09085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8662010.1"
                     /translation="MLGVGQKLPDFKIIGVKPGFNSHEENGVSAFEAVSQDSFEGKWK
                     VIFFYPKDFTFVCPTEIAEFASLARDFEDRDTVVLGGSTDNEFVKLAWRRDHADLSKL
                     PIWQFADNGGKFARDLGVLDEAEGVALRATFVVDPHNVVQHVYVTNLNVGRAPSDTQR
                     VVDALQTDELCPCNRQVGGETLAA"
BASE COUNT          296 a          615 c          614 g          278 t
ORIGIN      
        1 agaccacgac ggaaccggcg tcgaaggccg gatcggcttt gaagtcggaa cccatgcgcc
       61 gtcagttcag cccatagacg acgcaggccc ggtcggtcag ttcgcgcagc ggcggctgta
      121 gggccagccg ggcggcctcg atctgggcct cggacggcgc ggctcccggg ggcggaggcg
      181 gcggaggcgg agcggagggc gcgaactgcc gctccatacg cgacccggtg accgtgaccg
      241 catcgacgtc ggtcgccagc ccggagccgg acgcgtacga cggccagccg gccagcacat
      301 cggtctcgca ggcccggccg gtggggtcga tcacccgcac tccgcccagg gcggcgccgg
      361 cgttggacgc ggcctcacgg gcgcggcgcg ccgcatcctt gaccgcctcg ccctgcagcc
      421 aggtcttgac cgcattgtcg ggctccagat ggaacgacac cgggctggtc gacgtgggct
      481 gggccgcgag aacggtcgca tagacccgct cgaggacgga gccgtcccga acggtgacgc
      541 tgacattggc gttggcctga tagcgttcga tctgatcgcc gcgggcgttg tcgcgcagga
      601 cgccgttctc gtcacgatac tggtcgtaca gcggctgggt ggtcagactg gtctcgaccc
      661 gcaccttgtc gacgccatag gcggccagcg cctggctgag cgcgcgtacc tgatcggccg
      721 ccttgcgcga cgcttcggcg gccgagcggt cgatcgactg gaaggtggcc ccgaaactgg
      781 cgcggttggc cgcgacctcg gtgcggacgt agccgaccga ggcgatgacc ggatcgcgca
      841 tccaccaggg ggccggcaca tagcgctcgc cgatggtggc cgggggcgtc tgagctccag
      901 cgaggccggg agtcacgagg agcggcgccg cgctggcggc gatcagcagg acggcggtca
      961 gtcgacgcat ggtgtctgtt cctcttgata ttccagccga ccctggcggc tgaggctgcg
     1021 cccgtaaagc gcccaacggt ccagcaatcc tatgcgatgc ataggaaaaa tctatagaac
     1081 tatattgggt ccacacattg actttgtgca tcgcacaagc catttcgcgg ctgcggcgcc
     1141 gcctccccgg cgctcacaga ttccttcctt cagagacttc aggagaacag cgatgctggg
     1201 cgttggtcaa aaactgccgg acttcaagat catcggcgtc aagccgggct tcaacagcca
     1261 cgaggaaaac ggcgtctcgg cctttgaggc cgtctcgcag gacagcttcg aaggcaagtg
     1321 gaaggtcatc ttcttctatc cgaaggactt caccttcgtc tgcccgaccg agatcgccga
     1381 gttcgcctcg ctggcccgcg atttcgaaga ccgcgacacc gtggttctgg gcggctcgac
     1441 cgacaacgag ttcgtgaagc tggcctggcg ccgcgaccac gccgacctca gcaagctgcc
     1501 gatctggcag ttcgccgaca acggcggcaa attcgcccgc gacctgggcg tcctcgacga
     1561 ggccgagggc gtcgccctgc gcgccacctt cgtggtcgac ccgcacaacg tggtgcagca
     1621 cgtctatgtc accaacctga acgtcggccg cgcgccgtcc gacacccagc gggtggtcga
     1681 cgccctgcag accgacgagc tctgcccgtg caaccgccag gttggcggcg agaccctggc
     1741 ggcctagcgc tcagcagcgc gacgccagat gtccgggggc cggttcctcc ccccgcgccc
     1801 ccg
//
LOCUS       DASVVN010000561         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_74826, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000561 DASVVN010000000
VERSION     DASVVN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74826"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF686_09090"
     CDS             complement(<1..447)
                     /locus_tag="VF686_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX8662011.1"
                     /translation="MTGGPTGLDLKPARIDPIRAGLLCRCPQCARGPLFAGFLKVVDR
                     CAVCGFDFTRLNTGDGAAIFIMQIAGGIVVFGALFVQVAYNPPIQVMLAVALPLVAAL
                     SIGLMRPGKGVMIALQMRNMHSSSIGGGEPRSGGGGQPLTPMTAVGG"
     gene            complement(444..1352)
                     /locus_tag="VF686_09095"
     CDS             complement(444..1352)
                     /locus_tag="VF686_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8662012.1"
                     /translation="MADAHATPHHDYHLVNPSPWPLVASIAVTIMMVGAVVWMKGIVG
                     DDVGQLAATLLGKGQAALFFAGLAGVLVSMFGWWADVIKESKAGDHTPVVSIGLRYGM
                     MLFIASEVMFFVAFFWMFFEMAVFNEARAHIPEVANWADTAAAWSTWPPKGVETLDAW
                     QLPLLNTVILLLSGTTVTWAHHALQVGDRKAAKTGLLITVALGILFTAVQAYEYQHII
                     HENLFFNEEAANSGLYGSIFFMATGFHGFHVLIGTIFLLVCLLRLYAGQMSPAKHFGF
                     EAAAWYWHFVDVVWLFLFAFVYVTFG"
     gene            complement(1404..1949)
                     /locus_tag="VF686_09100"
     CDS             complement(1404..1949)
                     /locus_tag="VF686_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8662013.1"
                     /translation="MAIACVLGVMGMTGAAFAAVPLYRMFCQVTGFDGTVMKADKAPD
                     HILDETVLIRFDTNVRDLPMTFRAEQVSQRVRIGETGMAYFDVTNTSDQPIHATASYN
                     VVPERAGPYFQKLQCFCFDGQTIQPGETMKFPVQYFVAPEMATDRESQGVREITLSYT
                     FYPTKAAELAANSVQPTGAAG"
BASE COUNT          376 a          621 c          648 g          305 t
ORIGIN      
        1 ccccccgacc gcagtcatcg gggtcagggg ctggccccct ccaccgcttc gcggttcccc
       61 tcccccgatg gaggaggaat gcatgttgcg catctgcagg gcgatcatca cgccctttcc
      121 cggccgcatc aggccgatcg acagcgcggc gaccagcggc agggccacgg ccagcatgac
      181 ctggatcggc ggattgtacg cgacctgcac gaacagggcg ccgaacacca cgatcccgcc
      241 tgcgatctgc atgatgaaga tggcggcgcc gtcgccggtg ttcagccggg tgaagtcgaa
      301 gccgcagacc gcgcaccggt ccaccacctt cagaaagccc gcgaacaggg gccccctcgc
      361 gcattgcgga cagcggcaaa gcagaccggc gcggatcgga tcgatccgcg ccggcttaag
      421 atcaagccct gtggggccgc ccgtcacccg aaggtgacgt agacgaaggc gaagaggaac
      481 agccagacca cgtccacgaa atgccagtac caggcggcgg cctcgaagcc gaaatgcttg
      541 gccgggctca tctgccccgc atacaggcgc agcagacaga ccagcaggaa gatggtgccg
      601 atcaggacgt ggaagccgtg gaagcccgtc gccatgaaga agatcgaacc gtacagaccc
      661 gagttggcgg cttcctcgtt gaagaacagg ttctcgtgga tgatgtgctg gtactcatag
      721 gcctgcaccg cggtgaacag gatgccgagg gcgacggtga tcagcaggcc ggtcttggcg
      781 gccttgcggt cgccgacctg cagggcgtgg tgggcccagg tgacggtcgt gccggacagc
      841 agcaggatga cggtgttcag cagcggcagc tgccaggcgt ccagggtctc gacgcccttg
      901 ggcggccagg tcgaccaggc ggcggcggtg tcggcccaat tggcgacttc cggaatatgg
      961 gcgcgggctt cgttgaagac cgccatctcg aagaacatcc agaagaaggc gacgaagaac
     1021 atcacctcgg atgcgatgaa cagcatcatg ccgtagcgca ggccgatgga gacgaccggc
     1081 gtatgatcgc ccgccttgct ctccttgatc acatcggccc accagccgaa catcgacacc
     1141 agcacgccgg ccagaccggc gaagaacagg gccgcctggc ccttgcccag aagcgtcgcg
     1201 gccagctgac cgacgtcgtc gccgacgatg cccttcatcc acaccacggc gccgaccatc
     1261 atgatcgtga ccgcgatcga cgcgaccagc ggccaggggc tggggttcac caggtggtag
     1321 tcgtgatgcg gggtggcgtg agcgtcggcc atcagggtct cttgaagcgg tcgagatacg
     1381 tatggggcag gctatagcgt ccgctatccg gctgcgccag tgggttggac gctgttggcc
     1441 gccagctccg cggccttggt cggatagaag gtgtagctga gggtaatttc gcgcacgccc
     1501 tggctctcgc gatccgtcgc catctcgggc gcgacgaaat actggaccgg gaatttcatc
     1561 gtctcgccgg gctggatcgt ctgcccgtcg aagcagaaac actgcagctt ctggaaatag
     1621 ggcccggccc gttccggcac gacattgtat gacgccgtgg cgtgaatcgg ctgatccgag
     1681 gtgttggtca cgtcgaaata agccatcccc gtctcgccga ttcgcacccg ctggctgacc
     1741 tgttcggcgc ggaaggtcat gggcaggtcg cgcacattgg tgtcgaaccg gatcagcacc
     1801 gtctcgtcga gaatgtggtc gggcgccttg tcggccttca tcaccgtgcc gtcgaagccg
     1861 gtgacctgac aaaacatccg gtaaagaggc accgccgcga aggccgcgcc ggtcatgccc
     1921 atgacgccga gcacgcaggc gatggcgatg
//
LOCUS       DASVVN010000562         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_21893, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000562 DASVVN010000000
VERSION     DASVVN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21893"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF686_09105"
     CDS             complement(<1..249)
                     /locus_tag="VF686_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8662014.1"
                     /translation="MEFRHSDRALHWQERVGRFLEDHVLPREAEYRAQVRENSRRQPP
                     AMEEMKAKAREAGLWNMFLPGDHGAGLTNLEYAPLAELM"
     gene            complement(325..630)
                     /locus_tag="VF686_09110"
     CDS             complement(325..630)
                     /locus_tag="VF686_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8662015.1"
                     /translation="MADLAQVTEHIRGAVGDNSGLGKTVKIDLGDTGKIFIDGASVPN
                     TVTNEDKPADATVSIGWDDFMALSNGQLDPMMAFMQGKLKIAGDMMIAQKLAPLLKR"
     gene            complement(738..1349)
                     /locus_tag="VF686_09115"
     CDS             complement(738..1349)
                     /locus_tag="VF686_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662016.1"
                     /translation="MAAIAAVSVAALGLMGLKFVDLPGTAPVSPDEALKIEVVAPVEP
                     KPVPGSVMDVGELVDGFRYVPPRAAERPPLYDVAWTEDEDFASWAPPVRPAGVRRYNS
                     AEEAPAPSEPEPPRRERERRWFGFDNPLPDFGAERRAREARLDALEDQRRAELEDRAD
                     RRRYSSEPPRYQPPRDVGRNYNPPRAEPPADEVGEGPYGPEVG"
     gene            complement(1535..>1794)
                     /locus_tag="VF686_09120"
     CDS             complement(1535..>1794)
                     /locus_tag="VF686_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662017.1"
                     /translation="AGLVNAGEAGQPTARSNARPVLICATDAATRRAFTREHGAAPVF
                     VTAREALSVRSSDPAWSAPRCMTAREHSRYRDAATSYAAAR"
BASE COUNT          266 a          610 c          647 g          271 t
ORIGIN      
        1 catcagctct gccagcgggg cgtattcgag gttggtcagg cccgcgccgt gatcgccggg
       61 caggaacatg ttccacaggc cggcctcgcg cgccttcgcc ttcatctctt ccatggccgg
      121 aggttggcgc cgggagttct cgcggacctg agcccggtat tccgcctcgc ggggcaggac
      181 gtggtcctcg aggaaccgcc ctacccgctc ctgccagtgc agggccctgt cgctgtggcg
      241 gaattccatg gtcgtgctcc gatacgaaaa cggcgcggcc ccacaaggag ccgcgccgcg
      301 gaaatcagtt cgccgtcacg cgcctcagcg cttcagcagc ggggcaagct tctgggcgat
      361 catcatgtcg ccggcgatct tcagcttgcc ctgcatgaag gccatcatcg gatccagctg
      421 gccgttcgac agggccatga agtcgtccca gccgatcgac accgtcgcgt cggcgggctt
      481 gtcctcgttg gtgaccgtgt tgggcaccga ggcgccgtcg atgaagatct tgccggtgtc
      541 gcccagatcg atcttcacgg tcttgcccag accggagttg tcgcccacgg cgccgcggat
      601 gtgttcggtg acttgagcca ggtcggccat gggtctctcc ctgaaggacg ttgaaccatc
      661 gacctagacc gcgcggaagg cggcgccaag atcacgtcag gtcaagttgg cgcgacggat
      721 gcgctggtcc cgggcggtca gccgacctcc gggccgtaag ggccctcgcc cacctcgtcg
      781 gcgggcggtt cggcgcgcgg gggattgtag ttccggccaa cgtcgcgcgg cggctgatat
      841 cgaggcggct ccgaagagta gcggcggcga tccgcgcggt cctccagctc cgcccggcgc
      901 tgatcctcga gggcgtccag acgcgcctcc cgcgcgcggc gttcggcgcc gaagtccggc
      961 agcggattgt cgaagccgaa ccagcgacgc tcgcgctcgc gacgcggcgg ctcgggctcg
     1021 gacggcgcgg gagcctcctc cgcagagttg tagcgccgca ccccggcggg ccgcacgggc
     1081 ggcgcccagg atgcgaaatc ctcgtcctcg gtccaggcga cgtcatagag gggcggccgc
     1141 tccgccgcgc gcggcgggac atagcggaaa ccgtccacca gttcgcccac atccatcacc
     1201 gatccgggaa ccggcttggg ctcaacgggg gccacaacct cgatcttcag ggcctcgtcc
     1261 ggagacaccg gggccgtgcc gggaaggtcg acgaatttca gtcccatcag gcccagcgcc
     1321 gcgacgctga cggcggcgat ggcggccaca tgcgcgggtt tgatccgacg cggcccggac
     1381 gggacgggat cgatcggggg tcggggagga tttggaccgg gatacatggg aacaccagaa
     1441 ctgaagcggc gctcctgcaa ccccccagat caggtccggt tcaaaatcgc gatgccgacc
     1501 aggagcctca cccggatggc gcggcgaacc gcgcctagcg ggccgccgcg taggaggtcg
     1561 cggcgtcgcg atagcgggag tgctcacggg cggtcatgca gcgcggcgcg ctccaggccg
     1621 ggtccgagct ccggacggac agggcttccc gggcggtgac gaagaccgga gcggcgccgt
     1681 gttcccgggt gaaggcgcgg cgcgtcgcgg cgtcggtggc gcagatcagg acggggcggg
     1741 cgttcgaccg tgcggtcggc tgtccggcct cgccggcgtt caccagccct gcgg
//
LOCUS       DASVVN010000563         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_310228,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000563 DASVVN010000000
VERSION     DASVVN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310228"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="VF686_09125"
     CDS             complement(<1..1729)
                     /locus_tag="VF686_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662018.1"
                     /translation="MKTGIAQLPAARGLKGLLFGSVASLCLAAAAAPALAQSGAPGPA
                     ASGFLDYSAVERGPQIVIANPNTPTTALDPVNVTGVGQMIVDQQNGFIGTCTGTLINP
                     RTVLFAAHCVNDRAANAYGAGGTPIGFGFGNNNNAAGASAFGGWLADHRTNTARFMYN
                     SNYVAYNPASLEPNANSFLYGDVAIASLDTVARNIPTWSLLFSALPAAGGGAAGTGYH
                     VVLDGYGRNGTGTTGSTGSDYRRRLAENMLGGLASVDDFESFLFGSPGGDLPQNLYWI
                     DFDDPRRGTAQASPFDFNAWRDNALPREGITAAGDSGGPLILDRAFAKPVVIGVLSGG
                     YTRFFNGQPANGFGTASFYQPLYLYWDWIAANNPYHYVSSLAGDANWTDASHWVTNLD
                     PNYQIIGPNGQLVNGVPNDPGAGPRGPDANGYGQACFQSGGVSDCVDMATGVETIEAR
                     PIGTGATSGRAVVSIESLAEGDIDEGAAAGPQIGLDDGETVGVVREAQAGVAAQAGPS
                     ALPAATLLNGLPGATNFTPVNTDGNRLTSTPPRYFDVTLSANGTTTLNTAVTIDRLTM
                     AGVGAGLNIT"
     gene            complement(1862..2485)
                     /locus_tag="VF686_09130"
     CDS             complement(1862..2485)
                     /locus_tag="VF686_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8662019.1"
                     /translation="MAESPVSSVAKLRDRNATEAAIVAAGERILLRDGFPGLNVQTLA
                     AEAGCDRKLVYRYFDGADGVVDRIAARAHAGLTASLASIPVVETASLRTFARISLLTW
                     LAALRASPLSLRLMAWALVEDSDLLRRIEADRAAVLQNWMRERRPRLRVPPEGDPTAL
                     NAVLLAAVQQLALSADRRGGVAGLSLDADGWPRAEAALDRLLLAFPD"
     gene            2813..3250
                     /locus_tag="VF686_09135"
     CDS             2813..3250
                     /locus_tag="VF686_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX8662020.1"
                     /translation="MSLSLERLVYESTATGSTGSIGNLAVILAESQRNNDRDGLTGAL
                     AAHRERYIQVVEGPAQALDALLRRLEDDPRHRDVTLPDRQPISERLFNRWSMANARIT
                     PDHGAALDSLVDNDDLTPVQVIGILLDAVEKSEASRTAGLVRG"
BASE COUNT          522 a          991 c         1254 g          499 t
ORIGIN      
        1 tggtgatgtt cagcccggcg ccgacgcccg ccatggtcag gcgatcgatg gtgacggcgg
       61 tattgagcgt ggtcgtgccg ttggcggaca gggtcacatc gaaatagcgc ggcggggtgc
      121 tggtcagacg attgccgtcg gtgttgacgg gcgtgaagtt ggtcgcgccc ggcagaccgt
      181 tcagcagggt cgcggccgga agggcggacg gtccggcctg ggcggcgacc ccggcctggg
      241 cctcgcgcac gacgccgacg gtctcaccgt cgtcgagccc gatctgcggt ccggcggcgg
      301 cgccctcgtc gatgtcgcct tcagccaggg attcgatcga gacgacggcg cgaccgctcg
      361 tggcgccggt gccgatcggc cgggcctcga tggtctcgac gcccgtggcc atgtcgacgc
      421 agtcgctgac gccgccgctc tggaagcagg cctggccgta gccgttggcg tccggacccc
      481 gggggccggc gccggggtcg ttcggaacgc cgttcaccag ctgaccgttc ggcccgatga
      541 tctgatagtt cggatcgagg ttggtcaccc agtgggaggc gtccgtccag ttggcgtcgc
      601 ccgcgaggga cgagacgtag tggtacgggt tattggccgc gatccagtcc cagtacaggt
      661 agagaggctg gtagaaggag gcggtgccga agccgttggc gggctggccg ttgaagaagc
      721 gggtgtagcc gcccgacagc acgccgatga cgacgggctt ggcgaaggcg cggtccagga
      781 tcagcggtcc gccggagtcg cccgcggcgg tgatgccctc gcgcggcagg gcgttgtcgc
      841 gccaggcgtt gaagtcgaac ggactggcct gggccgtgcc gcgacgcggg tcgtcgaagt
      901 cgatccagta gaggttctgg ggcaggtcgc cgcccggcga tccgaacagg aagctctcga
      961 agtcgtccac cgacgccagg ccgccgagca tgttctcggc caggcgacgc cggtagtccg
     1021 atccggtgga cccggtggtg ccggttccat tgcggccgta gccgtcgagg accacgtggt
     1081 agccggtgcc ggcggctccg ccgccagcgg cgggcagggc cgaaaacagc agcgaccagg
     1141 tggggatgtt gcgcgccacc gtgtccagcg aggcgatcgc gacgtcgccg tagaggaagg
     1201 agttggcgtt cggttccagc gacgccggat tgtaggcgac gtagttggaa ttatacatga
     1261 agcgggcggt gttggtccgg tggtccgcca gccagccgcc gaaggcgctc gcgccggccg
     1321 cgttgttgtt attgccgaag ccgaagccga tgggcgtgcc gcccgcgcca taggcgttgg
     1381 ccgcccggtc attgacgcag tgggcggcga acaggactgt ccgcggattg atcagcgtgc
     1441 cggtgcaggt gccgatgaag ccgttctgct ggtcgacgat catctggccg acgccggtga
     1501 cgttcaccgg gtcgagcgcg gtcgtcggcg tgttcgggtt ggcgatgacg atctggggac
     1561 cgcgctcgac ggcggaatag tcgaggaagc cgctggcggc gggccccggc gcaccggact
     1621 gggcgagggc gggggctgct gcggcggcga gacacagact ggcgacagaa ccgaacagca
     1681 ggcccttcag gcctcgcgcg gcgggcagtt gagcgatccc ggtcttcatt ttgaaattcc
     1741 cccgtaagat tgaggctgaa atgtgcccac atccggaggg gaaagatcaa gcggttttcg
     1801 aagcttaacc gctttaaagt tgtagagaat tgcgcgcttg aggcgtcggc gccacgacag
     1861 gtcagtccgg gaaggcgagc agcaggcgat ccagcgcggc ctcggcgcgg ggccagccgt
     1921 cggcgtcgag gctcaggccg gcgacgccgc cgcggcggtc ggcggacagg gccagctgct
     1981 gcacggcggc cagaaggacg gcgttgaggg cggtggggtc gccctcgggc gggacgcgca
     2041 gccgggggcg gcgttcgcgc atccagttct gcagcacggc ggcgcggtcg gcctcgatgc
     2101 ggcgcagcag gtcggagtcc tcgaccaagg cccaggccat cagccgcagg ctcagcggac
     2161 tggcgcgcag ggccgccagc caggtcagca ggctgatgcg tgcgaaggtc cgcagggagg
     2221 cggtttcgac caccgggatg gaggcgaggc tggcggtcag tcccgcgtgg gcgcgcgcgg
     2281 cgatgcggtc gaccacgccg tcggcgccgt cgaaatagcg atagaccagc ttgcggtcgc
     2341 agccggcctc ggcggccagg gtctggacgt tcaggccggg aaagccgtcg cgcagcagaa
     2401 tgcgttcgcc cgccgcgacg atggcggcct cggtggcgtt tcggtcgcgc agcttcgcga
     2461 cggaggagac gggcgattcg gccatgcgcc cagcatcggg ccgcgcgacg tgagtcggca
     2521 agcgatggcg gacaggctgt tccggaagaa gatgcggccc ggtctgcgtc ggggggcgtg
     2581 gcagagaccg ggccgctgag gactctgtcg agcgcgcatc gccgcaaggt ccgatgggaa
     2641 aactgcggtc cgcgacatcg gttcctcgcc cgataaaaaa atttcgggag gggcggcccc
     2701 ggcgtgctgg cctaggtcgg gaaatggata ttcctgttca aacacaaaac tctataccga
     2761 ctgcaaccgg aatggttgta tccgcattat cggcggacag tttgagggtc gcatgagttt
     2821 gagtctggaa cggctggtct atgaaagcac cgccacaggg agcacggggt ccatcgggaa
     2881 cctcgccgtc attcttgcgg agtcacagcg gaataacgac cgcgacggcc tgaccggcgc
     2941 cttggccgcg catcgggagc gatacatcca ggtcgtggag ggcccggcgc aggcgctgga
     3001 cgccctgctg cggcggctgg aggacgatcc ccggcaccgc gacgtcaccc tgccggaccg
     3061 ccagcccatc tcggagcggc tgtttaaccg gtggtcgatg gctaatgcgc ggatcacgcc
     3121 ggaccatggc gcggcgctgg acagtctggt cgacaacgac gatctcacgc ccgtgcaggt
     3181 gatcggaatc ctgctggatg cggtcgagaa gagcgaggcg agccggacgg ccggactcgt
     3241 tcgcggctga gaacatcagc gaaccg
//
LOCUS       DASVVN010000564         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_310304,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000564 DASVVN010000000
VERSION     DASVVN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310304"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VF686_09140"
     CDS             complement(<1..1158)
                     /locus_tag="VF686_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8662021.1"
                     /translation="MKSITSFMAAGAIFLATSVAAQTPAPAPASAGEGTTFVQVGRLL
                     ADPASGVVQRDKTLVIRGNQIAEVRDGFVGEGRIVDLRDSFVLPGLIDSHVHLTGEQN
                     PNGRLEAVTQSNAEQAMVGARYARRTLMAGFTTVADLGATNESIFALRDAVRRGDVPG
                     PRIIASGSAVSVHGGHGDINGYREDVMHLLSDESVCSGVDDCMRAVRTQVRSGADIIK
                     ITATGGVLSNTAAGLGQQFSDEELAGIVGAAHRMGRLVTAHAHGADGINSFLRAGGDS
                     IEHGTYLDDESIRLMRRDGVYLVPTLMAGDFVARIASGPNNFFTPAQTAKALEAGPKM
                     LDMARRAHEGGVRIAFGTDTGVSAHGDNAGEFALLVRAGMTPLETIQAATVN"
     gene            1280..1993
                     /locus_tag="VF686_09145"
     CDS             1280..1993
                     /locus_tag="VF686_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEX8662022.1"
                     /translation="MRLARRGFILGGAALLSGCATASAATQTAAFRPLPGLTQTPVPT
                     VVIAPTPEVITAELNTTRPLDPRGQIRKELMDRAMAALDIHKGRITQRDRMYLVDFQK
                     FSGDERLYEVDLHGGAVTLMRTSHGRGSDPAHTGFAREFGNTPDSYKSSVGAYATAGA
                     GFGAQQGPNVLLDGLEYSNDRARERAIIIHGADYADPAFLAREGKLGRSYGCFSVAHA
                     DLPRLRERMGEGRLLFAWA"
     gene            complement(1998..2657)
                     /locus_tag="VF686_09150"
     CDS             complement(1998..2657)
                     /locus_tag="VF686_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX8662023.1"
                     /translation="MTDALPPEPPSHYRDRAERLADLIVHLTGLALALVGGGMLLGLA
                     IGFGHVGRLTAAAIYAVGLLTMLGLSTAYNFAAARFQPLLRRFDHAGIFVMIAGSYTP
                     FTTQHLTGAWAWGMTSAVWGVAIFAALAKLFLPGLGKGFWIVVYLALGWLMVIAIQPI
                     LQSLGLAPLILMAVGGVLYSVGVIFYANKAMRFRRAIWHGHVVVAAAVHWVAVLLAVM
                     K"
     gene            complement(2650..>3004)
                     /locus_tag="VF686_09155"
     CDS             complement(2650..>3004)
                     /locus_tag="VF686_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX8662024.1"
                     /translation="ACARGDLTRAPMPAFRDETVICVVLAASGYPDSPLEGSIIRGAE
                     QDFGPHVQVFHAGTRRRADRQLAAAGGRVLNVCARGKDIAEARERAYAAVKKIDWPGG
                     FHRTDIGWRAMEPRD"
BASE COUNT          499 a         1134 c          951 g          420 t
ORIGIN      
        1 attgaccgtc gccgcctgga tggtttccag cggcgtcatc ccggcccgaa ccagcagggc
       61 gaactcgccc gcgttgtcgc catgggccga aaccccggtg tccgtgccga acgcgatccg
      121 caccccgccc tcatgcgcgc ggcgcgccat gtccagcatc ttcggccccg cctccagcgc
      181 cttggccgtc tgggccgggg tgaagaaatt gttcggaccc gaggcgatcc gcgccacgaa
      241 atcgccggcc atcagcgtcg gcaccagata gacgccgtcg cgccgcatca gccggatcga
      301 ctcgtcgtcc agatacgtcc catgctcgat cgagtcgccg cccgcccgca ggaagctgtt
      361 gatcccgtcc gcgccgtgcg catgcgccgt caccagacgg cccatccggt gcgccgcccc
      421 gacgatgccg gccagttcct cgtccgagaa ctgctggccc agacccgccg ccgtattcga
      481 cagcaccccg ccggtggcgg tgatcttgat gatgtcggcg cccgaccgca cctgggtccg
      541 caccgcccgc atgcagtcgt cgacgcccga gcagacgctc tcgtccgata gcaggtgcat
      601 gacgtcttcg cggtagccgt tgatgtcgcc gtgcccgccg tggaccgaca ccgccgaccc
      661 cgaggcgatg atccgcggcc ccggcacgtc gccgcgccgc acggcgtctc tcagggcgaa
      721 gatggactcg ttggtggcgc ccagatcggc gaccgtggtg aagccggcca tcagggtgcg
      781 ccgcgcataa cgggccccaa ccatggcctg ttcggcattg gactgggtga ccgcctccag
      841 ccgcccgttc gggttctgct cgccggtcag atgcacatgg ctgtcgatca gtccgggcag
      901 cacgaacgag tcgcgcagat cgacgatccg gccctcgccg acgaagccgt cgcgcacttc
      961 cgcgatctgg ttccctctga tcacgagagt tttatcgcgc tgcacgacgc cgctcgcagg
     1021 atcggccaga agtcgcccga cttggacgaa cgtagttccc tcgccggccg aagcgggcgc
     1081 gggagcgggc gtttgcgccg caacgctcgt agccaggaaa atggcgcccg ccgccataaa
     1141 tgatgtgatc gacttcacga aaccctcctc gaaacaggcg cgcaccttag acgtccgggc
     1201 cgtttcgcca aggctcaggt tgatccaccc gtaacgacct gaaacaaccc gtgctccaga
     1261 aacgtgcgag gggcgtcgca tgcggctagc gagacgtgga ttcatactgg gcggcgcggc
     1321 gctgctgtcg ggctgcgcca ccgcatctgc ggctacgcag acggcggcct tccggcccct
     1381 gcccggcctg acgcagaccc cggttccgac ggtcgtcatc gcgccgacgc ccgaggtcat
     1441 cacggccgag ctgaacacca cgcgtccgct cgatccccgc ggccagatcc gcaaggaact
     1501 gatggaccgc gccatggccg ccctcgacat ccacaagggc aggatcacgc agcgcgaccg
     1561 catgtacctg gtcgacttcc agaaattctc gggcgacgag cgcctgtacg aggtcgatct
     1621 gcacggcggc gccgtcaccc tgatgcgcac cagccacggc cgcggctccg atcccgcgca
     1681 caccggcttc gcccgcgagt tcggcaatac gcccgacagc tacaagtcct cggtcggcgc
     1741 ctacgccacc gccggagccg gcttcggggc ccagcagggg ccgaacgtcc tgctggacgg
     1801 actggagtat tccaacgacc gcgcccgcga acgcgccatc atcatccacg gcgccgatta
     1861 cgccgacccg gccttcctcg cccgcgaggg caagctgggc cgcagctacg gctgcttctc
     1921 ggtcgcccac gccgacctgc cccgcctgcg cgagcgcatg ggcgaaggcc ggctgctgtt
     1981 cgcctgggcc tgagacccta cttcatcacc gccagcagca cggcgaccca gtgcacggcc
     2041 gccgcgacca cgacatggcc gtgccagatc gcacgccgga agcgcatggc cttgttggcg
     2101 tagaagatca cgccgacgga atagagcacg cctccgaccg ccatcaggat cagcggcgcc
     2161 agaccgaggc tctgcagaat gggctggatg gcgatcacca tcagccagcc gagcgccaga
     2221 tagacgacga tccagaatcc cttgcccagt ccgggcagga acagcttggc cagggccgcg
     2281 aaaatcgcga ccccccacac cgccgacgtc atcccccagg cccaggcgcc cgtcagatgc
     2341 tgcgtcgtga acggcgtgta ggatccggcg atcatgacaa agatgcccgc atggtcgaag
     2401 cgccggagca gcggctggaa gcgggcggcg gcgaaattat aggccgtcga caggcccagc
     2461 atggtcagca gcccgaccgc atagatcgcc gccgcggtca gccgccccac atggccgaac
     2521 ccgatcgcca gccccagcag cattcccccg ccgacgagcg cgagcgccag tccggtcaga
     2581 tggacgatca ggtcggccag acgctccgcc cggtcccggt aatggctcgg cggctcgggc
     2641 ggcagggcgt cagtcacggg gttccatcgc ccgccagccg atgtcggtgc ggtggaaccc
     2701 gcccggccag tcgatcttct tgaccgccgc ataggcccgc tcgcgcgcct cggcgatgtc
     2761 cttgccccgc gcgcagacgt tcagcacccg gccgcccgcc gccgccagct gccggtcggc
     2821 ccgccgccgg gtgcccgcgt ggaagacctg cacatgcggg ccgaaatcct gctcggcccc
     2881 gcggatgatc gacccctcca gcggactgtc gggataaccc gaggccgcca gcacgacgca
     2941 gatcaccgtc tcgtcgcgga aggccggcat cggcgcccgc gtcaggtcgc cccgcgcgca
     3001 ggcc
//
LOCUS       DASVVN010000565         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_204875,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000565 DASVVN010000000
VERSION     DASVVN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204875"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF686_09160"
     CDS             <1..499
                     /locus_tag="VF686_09160"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8662025.1"
                     /translation="YVPESSPESNAALTKRTLMTGPAAAARFVPLYRPEVTVDRAAAL
                     AAHALTAEGGHLDAVLLGMGEDGHICSMFPESPTLKTLLSPGLRPTVLGVPTGRDGRG
                     PTIERLSINLPYLMQARRVVLAITGPAKRAVFEREAGGDPSIQPIAALLAHNVPLEVV
                     WTEKA"
     gene            499..2391
                     /gene="edd"
                     /locus_tag="VF686_09165"
     CDS             499..2391
                     /gene="edd"
                     /locus_tag="VF686_09165"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX8662026.1"
                     /translation="MTRLNPVVAEVTARIVERSRATRADYLRRMDAARDSGAGRAKLS
                     CANWAHAFAGNPIRDKLTAMTGKEPNVGVVTAYNDMLSAHQPFERFPHIIRDAVREVG
                     ATAQVAGGTPAMCDGVTQGRPGMELSLFSRDVIAMSTGVALTHDAFDSVICLGVCDKI
                     VPGLFMGSLAFGHLPVVFAPAGPMPSGIPNAEKARVRALYAQNKVDRQTLLDSEIGSY
                     HSPGTCTFYGTANSNQMMMELGGLHMPSTAFVHPETGLRDALTAAAAKRAVELARTGQ
                     GRMADVVDEKSVVNMIVALLATGGSTNHAIHLVAMARAAGVLIDWTDMDQLSSVTPLL
                     ARVYPNGSADVNAFQAAGGVAFVARELAGAGGIHSDVTTIMGEGIHHYFQEPAMIDGE
                     LVWKDGVTESLDTDILRPASDPFDTEGGLRLVQGDLGRAVIKVSAVKPENRIVEAPAA
                     VFETQEDALQAFRDGALNRDVVVVLRFQGPKANGMPELHSLSPALGVLQDKGFKVAFV
                     TDGRMSGASGKTPAAIHVSPEALAGGPLAHVRDGDIIRLDADAGVLSTIGVPELTTRP
                     AAARSQVHAEGSGWGYGRELFAAFRHVVSTAEEGASVCFAPPVGSPAQVDTPPDNNLV
                     DRAVDA"
     gene            2444..3421
                     /gene="glk"
                     /locus_tag="VF686_09170"
     CDS             2444..3421
                     /gene="glk"
                     /locus_tag="VF686_09170"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX8662027.1"
                     /translation="MTIAWDRTLLVGDVGGTNARFALAHMVEGKPVLEHHDSFPAETY
                     PTFLEGVRAFIDGCEVKPTGGVIAVAGPVTDGEIDLTNSPWRVSEAELQTLGMNPVRL
                     INDFEALAWGAPVVPADQLASLGGPGEGEPHAAIAVLGPGTGFGVSALARDIHGTEIA
                     MPSEGGHACFAPGDAVEDEILRILRRRYDRVSIERLICGPGLLNMHRALAEIDGRESH
                     IDDPAQITTEAMEDPNSPCGATLARFCAILGAVAGDIALTTGARGGVYIAGGIAPRIL
                     PFLKASPFRERFERKGRFKDYMAAIPTHVILHKHAALLGAARVAFAEEK"
     gene            3506..>3751
                     /locus_tag="VF686_09175"
     CDS             3506..>3751
                     /locus_tag="VF686_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662028.1"
                     /translation="MRKLIIASAAIFALAGVAACESQADKTAEVQADALEEQAAAAPT
                     EAAETALNEKADLVEQQAGNADGGATTANTPNTSPSTA"
BASE COUNT          595 a         1468 c         1150 g          538 t
ORIGIN      
        1 ctacgttccc gagagctcgc ccgaatccaa cgccgcgctg accaaacgga ccctgatgac
       61 cggccccgcc gccgcggccc ggttcgtgcc cctgtatcgt cccgaggtca ccgtcgaccg
      121 cgccgccgcc ctcgccgccc acgccctcac cgccgaggga ggacatctcg acgcggtcct
      181 tctcggcatg ggggaggatg gccatatctg ctccatgttc cctgaaagcc ccaccctcaa
      241 gaccctgctg tcgccgggcc tgaggccgac cgtgctcggc gtgcccaccg gccgcgacgg
      301 acgcggcccc accatcgaac gcctgtcgat caacctgccg tatctgatgc aggcccgccg
      361 cgtggtcctc gccatcaccg gccccgccaa gcgcgccgtg ttcgaacgcg aggccggcgg
      421 agatccgagc atccagccga tcgccgccct cctcgcccac aacgtcccgc tggaagtcgt
      481 ctggacggag aaggcctgat gacccgattg aaccccgtcg tcgccgaggt caccgcccgc
      541 atcgtcgagc gctcgcgcgc cacccgcgcc gactatctgc gccgcatgga cgccgcccgc
      601 gacagcggcg ccggccgcgc caaactcagc tgcgccaact gggcccacgc cttcgccggc
      661 aatccgatca gggacaagct gacggcgatg accggaaagg agcccaacgt cggcgtcgtc
      721 accgcctaca acgacatgct ctcggcccat cagccgttcg agcgcttccc gcacatcatc
      781 cgcgacgccg tgcgcgaggt cggcgccacg gcgcaggtcg ccggcggcac gcccgccatg
      841 tgcgacggcg tcacgcaggg gcgcccgggc atggagctgt cgctgttcag ccgcgacgtc
      901 atcgccatgt cgaccggcgt cgccctgacc catgacgcct tcgactccgt catctgcctc
      961 ggcgtctgcg acaagatcgt gcccggcctg ttcatgggct cgctcgcctt cggtcacctg
     1021 ccggtcgtct tcgccccggc cggtcccatg ccctccggca ttcccaacgc cgaaaaggcc
     1081 cgcgtccgcg ccctctacgc ccagaacaag gtcgaccgtc agaccctgct ggacagcgaa
     1141 atcggcagct accattcgcc cggcacctgc accttctacg gcacggccaa ctccaatcag
     1201 atgatgatgg agctgggcgg cctgcacatg ccctcgaccg ccttcgtcca ccccgagacc
     1261 ggcctgcgcg acgcgctgac ggcggccgcg gccaaacgcg ccgtcgaact ggcccggacc
     1321 ggtcagggcc ggatggccga cgtcgtggac gagaagtcgg tcgtcaacat gatcgtcgcc
     1381 ctgctggcca cgggcggctc gaccaaccac gccatccatc tggtcgccat ggcgcgggcg
     1441 gcgggcgtgc tgatcgactg gaccgacatg gaccagctca gctcggtcac ccccttgctg
     1501 gcgcgggtct acccgaacgg ctcggccgac gtgaacgcct tccaggccgc cggcggcgtc
     1561 gccttcgtgg cccgcgaact agccggcgcc ggcggcatcc attccgacgt caccaccatc
     1621 atgggcgagg gcatccacca ctatttccag gagccggcga tgatcgacgg cgaactggtc
     1681 tggaaggacg gcgtgaccga aagtctggac acggacattc tgcgccccgc ctccgatccc
     1741 ttcgacaccg agggcggcct gcgccttgtc cagggcgacc tcggccgggc cgtcatcaag
     1801 gtctcggcgg tcaagcccga gaaccgtatc gtcgaggcgc ccgccgccgt cttcgaaacc
     1861 caggaagacg ccttgcaggc cttcagggac ggcgcgctga accgcgacgt cgtggtcgtc
     1921 ctgcgcttcc agggccccaa ggccaacggc atgcccgagc tgcacagcct gtcgcccgcc
     1981 ctcggcgtgc tgcaggacaa gggcttcaag gtcgccttcg tcaccgacgg ccgtatgtcg
     2041 ggcgcctcgg gcaagacacc cgccgccatc cacgtctcgc ctgaagccct cgccggcggg
     2101 cctctggccc acgtccgcga cggcgacatc atccgcctcg acgccgacgc cggcgtgctc
     2161 agcaccatcg gcgtccccga gctgaccacc cgccctgccg cggcgcgctc tcaggttcac
     2221 gccgagggct cgggttgggg ttacggtcgg gagcttttcg ccgccttccg tcatgtcgtc
     2281 tcgaccgccg aggagggcgc ctccgtctgt ttcgccccgc ccgtcggctc gccggcccag
     2341 gtcgacacgc cgcccgacaa caacctcgtc gaccgcgcgg tggacgcatg acgccccacc
     2401 ctcaagcagt gagccgccgc gcggcgaacg tcagggaaca aacatgacca tcgcctggga
     2461 caggaccctc ctcgtcggcg acgtcggcgg caccaacgcc cgcttcgccc tggcccatat
     2521 ggtcgagggc aagccggtgc tcgaacacca cgacagcttc ccggccgaga cctacccgac
     2581 cttccttgag ggcgtgcgcg ccttcatcga cggctgcgag gtcaagccga ccggcggcgt
     2641 catcgccgtg gcgggaccgg tcaccgacgg cgagatcgac ctgaccaatt cgccatggcg
     2701 cgtgtccgag gcggaactcc agaccctggg catgaatccg gtccgcctga tcaacgattt
     2761 cgaggctctg gcctggggcg cgcccgtcgt gcccgccgac cagttggcct cgctgggcgg
     2821 ccccggggaa ggcgagccgc acgccgccat cgccgtgctc ggtccgggca ccggcttcgg
     2881 cgtctcggct ctcgcccgcg acatccacgg aaccgagatc gccatgccct ccgagggcgg
     2941 ccacgcctgt ttcgcgcccg gcgacgccgt tgaggacgag atactgcgca tcctgcgccg
     3001 ccgctatgac cgcgtctcga tcgaacggct gatctgcggc cccggcctgc tgaacatgca
     3061 ccgcgcgctc gccgagatcg acggccgcga aagccatatc gacgacccgg cccagatcac
     3121 caccgaggcc atggaggatc ccaacagccc atgcggcgcc accctggccc gcttctgcgc
     3181 catcctcggg gccgtggccg gcgacatcgc cctgaccacc ggcgcacgcg gcggcgtcta
     3241 tatcgccggc ggtatcgcgc cgcggatcct gcccttcctc aaggccagcc ccttccgcga
     3301 acggttcgag cgcaagggcc ggttcaagga ctatatggcc gccatcccga cccatgtgat
     3361 cctgcacaag cacgccgccc tgctgggcgc cgcccgcgtc gccttcgccg aagaaaagta
     3421 gggttcccta catttttctt cacggacgcg tgaaccgaag cgtcgctgca gagttatccg
     3481 gtctccactc tggaaggata tcctcatgcg taaactgatc atcgcctccg ccgccatctt
     3541 cgctctggcc ggcgtcgccg cctgcgagag ccaggccgac aagaccgccg aagttcaagc
     3601 cgacgccctg gaagaacagg ccgccgccgc tccgaccgaa gccgctgaaa cggccctgaa
     3661 cgagaaggcc gacctggtcg agcagcaagc cggcaacgcc gacggcggcg cgaccaccgc
     3721 caacaccccg aacacctcgc cgtcgacggc g
//
LOCUS       DASVVN010000566         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_337788,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000566 DASVVN010000000
VERSION     DASVVN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337788"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1599)
                     /gene="cysS"
                     /locus_tag="VF686_09180"
     CDS             complement(190..1599)
                     /gene="cysS"
                     /locus_tag="VF686_09180"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX8662029.1"
                     /translation="MPLTLHDTLHREKRVFTPRNPDRVTLYVCGPTVYDYAHIGNARP
                     PVVFDVLVRLLRREYGADRVIYARNVTDVDDKINAKATKEGTPIGDVTSRYEAAYLDD
                     MSRLNVSPPDLAPHVTDHMDAIVKQIGAIMDQGCAYAAEGHVLFDVSSYGTYGKLSGR
                     NLDDMIAGARVEVAPYKKNPHDFVLWKPSKEGEPSWPSPWGEGRPGWHIECSAMIEET
                     LGLPIDIHGGGIDLVFPHHENEIAQGVCGHGHAHEPNAHAEYSRYWMHNGFLTVDAEK
                     MSKSIGNVLLLHDLVQNIPGEVVRWALLSAHYRQPLDWNQSLLDQSRKALDRLYGALH
                     RAAAVEANGDEPPGDFLKAIEDDINTPGAMATLFALSSEIERAMTAGDMGVVARAKGE
                     LIAAAAILGVLQADPAGWLEGEVSDGLRTEVEALLEQRAAARAGKDWTEADRIRDRLN
                     ALNVVVMDGPTGATWRVKA"
     gene            1692..2312
                     /gene="folE"
                     /locus_tag="VF686_09185"
     CDS             1692..2312
                     /gene="folE"
                     /locus_tag="VF686_09185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8662030.1"
                     /translation="MSVTPVKPVLVGDNDPAPRPSREKALEAVRTLLAWAGDNPDRPG
                     LIDTPRRVVDAYGEWFDGYDADVEKELSRTFEDVQGYDDIVMLREIEVESHCEHHMAP
                     FLGKAYVAYMPTNRVVGISKIARVVEIFARRLQTQETMTQQIADAITDSLRPAGVAVL
                     IDAAHQCMTTRGVHHKNVSTITTQFTGVFKTDPALQDRFLRFTGRS"
BASE COUNT          384 a          810 c          744 g          395 t
ORIGIN      
        1 cccaaaccgg acagcccgcg gcgcggacgt gcaggcggcc gccagcatcg tcagcaagag
       61 cagtacgctg gtcgcggccg cacgattgac gtggttcatt ttgccccccg agtgcaggat
      121 tgaacctgaa cgaattgccg ctcagctaac ccggttggct ggcgctcgca aaccgtcggg
      181 attccggcgt caggccttta cccgccaggt cgcccccgtc ggtccgtcca tgaccaccac
      241 gttcagggcg ttcagccggt cgcggatgcg gtcggcctcg gtccagtcct tgccggcgcg
      301 ggcggccgcg cgctgttcca gcagagcctc gacctcggtg cgcaggccgt cggagacctc
      361 gccttccagc catccggccg gatcggcctg aagcacgccc agaatggccg cggcggcgat
      421 cagttcgccc ttggcccggg ccaccacgcc catgtcgccg gccgtcatgg cgcgctcgat
      481 ctcgctggac agggcgaaca gggtcgccat ggccccgggg gtgttgatgt cgtcctcgat
      541 ggccttgagg aaatccccgg gcggctcgtc gccattggcc tcgacggcgg cggcgcgatg
      601 gagggcgccg tacagccggt ccagcgcctt gcggctctgg tccagcagcg actgattcca
      661 gtccagcggc tggcggtaat gtgcgctcag cagggcccaa cggacgacct cgcccggaat
      721 attctgaacc agatcgtgca gcagcaggac gttgccgatc gacttggaca tcttctccgc
      781 gtcgacggtc aggaagccgt tgtgcatcca gtagcggctg tattccgcat gggcgttcgg
      841 ctcatgcgca tggccgtggc cgcagacgcc ctgggcgatc tcgttctcat ggtgcggaaa
      901 caccagatcg atgccgccgc cgtggatgtc gatcggcagg cccagcgtct cctcgatcat
      961 ggccgagcat tcgatatgcc agcccggacg cccctcgccc cacggtgacg gccacgaggg
     1021 ctcgccctcc ttcgacggct tccacagcac gaagtcgtgc ggattcttct tgtacggcgc
     1081 gacctcgacc cgggccccgg cgatcatgtc gtccaggttc cggcccgaga gcttgccgta
     1141 ggtcccgtag gacgagacgt cgaacagcac atggccctcg gcggcgtagg cgcagccctg
     1201 atccatgatc gcgccgatct gcttcacgat ggcgtccatg tggtcggtga cgtgcggcgc
     1261 gagatccggc ggcgagacgt tcagccggct catgtcgtcc agataggccg cctcgtaacg
     1321 cgacgtgacg tcgccgatgg gcgtgccttc cttcgtcgcc ttcgcattga tcttgtcgtc
     1381 cacgtcggtg acgttgcggg cgtagatcac cctgtccgcg ccatattccc ggcgcagaag
     1441 ccggaccagc acgtcgaaca ccaccggcgg ccgggcattg ccgatatggg cgtagtcata
     1501 gaccgtgggg ccgcagacat agagggtcac ccggtccgga tttcgcggcg tgaacacgcg
     1561 cttttcgcgg tgcagggtgt cgtgaagtgt cagcggcata gtgaggcggt tgctttcagt
     1621 tttcttgcag gacgcacctg cgctcccata tagccgtgct gctgcacaag cgaacagcgt
     1681 cgagatcgag aatgagcgtc acccccgtca agccggttct ggtcggagac aacgaccccg
     1741 ccccgcgccc ctcgcgcgaa aaggcgctag aggcggtgcg caccctgctg gcctgggccg
     1801 gcgacaatcc cgaccgaccc gggctgatcg acacaccccg ccgcgtggtc gacgcctatg
     1861 gcgaatggtt cgacggctat gacgccgacg tcgagaagga gctgagccgc accttcgagg
     1921 acgtgcaggg ctacgacgac atcgtcatgc tgcgcgagat cgaagtggag agccactgcg
     1981 agcaccacat ggcgcccttc ctcggcaagg cctacgtggc ctacatgccg accaaccgcg
     2041 ttgtcggcat ctccaagatc gcccgggtgg tcgagatctt cgcccgccgt ctccagaccc
     2101 aggagaccat gacccagcag atcgccgacg ccatcaccga cagcctgcgc ccggccggcg
     2161 tcgcggtgct gatcgacgcg gcccaccagt gcatgaccac ccgcggcgtg caccacaaga
     2221 acgtgtcgac catcaccacc cagttcaccg gcgtgttcaa gacggacccc gccctgcagg
     2281 accgcttcct ccgcttcacc ggccgcagct gatcgccgaa cctttcggaa gct
//
LOCUS       DASVVN010000567         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_127125,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000567 DASVVN010000000
VERSION     DASVVN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127125"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VF686_09190"
     CDS             <1..741
                     /locus_tag="VF686_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8662031.1"
                     /translation="QMGLVQAYGIPGGYGWNAPSWSISALIGCYLAFPWILKGLVRLG
                     PWSVLALGVGLYLLANQLTWSLFGYPVYQMPMGFGFFRALPLFFLGMSLAWFAQKVWI
                     APKLARWAGVAAALGLAVAQYFDKNALISLACISIIILAAGAIPTPRPSKWIEKAAQV
                     SFSMFITNEVVRIAWFGVANVAIARFALPVPAQWALWAGGVAAAFAFAWLFHVTVDTP
                     LQDRIRVWLKSRSRRAPAGLRPVVSLEG"
     gene            complement(738..1187)
                     /locus_tag="VF686_09195"
     CDS             complement(738..1187)
                     /locus_tag="VF686_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8662032.1"
                     /translation="MIQTRHGSCHCGAVKYECDIDLAPPGQRSEQIRPGPWFASTLRC
                     NCTFCGKTRMWKNHTPAEAFRLLQGADQLTSYRFGEGTIDHTFCKVCGVYPFASASME
                     PLGGDFYCVNIATLDDVTPEEFEAAPIVHEDGINDDWTHPPKVTGYL"
     gene            complement(1187..1570)
                     /locus_tag="VF686_09200"
     CDS             complement(1187..1570)
                     /locus_tag="VF686_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8662033.1"
                     /translation="MLKDRNSSAIVAVRDIDRARTFYEETLGLTLEQSDTEGGVLGFR
                     TGDTRLTVYKSDFAGTNKANAVSWDMDGDLVETVQALRTKGVVFEHYPDMGMTLNGDI
                     HEIGGMKLVWFKDPDGNILHLIEGM"
     gene            complement(1627..>1929)
                     /locus_tag="VF686_09205"
     CDS             complement(1627..>1929)
                     /locus_tag="VF686_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662034.1"
                     /translation="IAMGSDLYFPYGAGRGAGARATMDGYVEAGLSAAEALQTATWEA
                     GRLLGDDGLGVVKARSWADLVAVHGDPTATLEPLRTPRLVLKGGRIEAGSPETCAG"
BASE COUNT          271 a          654 c          629 g          375 t
ORIGIN      
        1 cagatgggtc tggtgcaggc ctacggcatt cccggcggct atggctggaa cgcgcccagc
       61 tggtcgatct cggccctgat cggctgctac ctggccttcc cctggatcct caaagggctg
      121 gtgcggctgg gcccgtggag cgtactggcg ctgggcgtcg gcctttatct gctggccaac
      181 cagctgacct ggagcctgtt cggctatccc gtctaccaga tgcccatggg cttcggcttc
      241 ttccgggccc tgccgctgtt cttcctcggc atgtcgctgg cctggttcgc ccagaaggtc
      301 tggatcgcgc cgaagctggc gcgatgggca ggtgtcgcag ccgcgctcgg tctcgccgtg
      361 gcgcaatact tcgacaagaa cgccctgatc tcgctggcct gtatctcgat catcattctg
      421 gcggcgggcg ccattccgac gccgcggccc tcgaaatgga tcgagaaggc ggcgcaggtt
      481 tcgttctcga tgttcatcac caacgaggtc gtgcgcatcg cctggttcgg cgtcgccaat
      541 gtcgccatcg cccggttcgc cctgcccgtc cccgcccagt gggcgctgtg ggccggcggc
      601 gtggcggcgg ccttcgcctt cgcctggctg ttccacgtca ccgtcgacac accgcttcag
      661 gaccggatcc gcgtctggct gaagagccgt tcgcgtcgtg cgccggccgg gctgcggccc
      721 gtggtgtcgc tggaaggcta aaggtagccc gtcaccttcg gcgggtgggt ccagtcgtcg
      781 ttgatcccgt cctcatggac gatgggcgcc gcctcgaact cctcgggcgt cacatcgtcc
      841 agagtggcga tgttgacgca atagaagtcg ccaccgagcg gctccatgct ggcgctggcg
      901 aagggataga cgccgcacac cttgcagaag gtgtggtcga tcgttccttc cccgaaccgg
      961 taggacgtca gttgatccgc gccctgcagc aggcggaagg cttcggccgg ggtgtggttc
     1021 ttccacatcc gggtcttgcc gcagaacgtg cagttgcagc gcagggtcga ggcgaaccac
     1081 ggacccggcc ggatctgttc cgagcgctga ccgggcggcg cgagatcgat gtcgcactcg
     1141 tacttcacag cgccgcaatg gcagctgccg tgccgggtct ggatcatcac atgccttcga
     1201 tgaggtggag gatgttgccg tcgggatcct tgaaccacac cagcttcatc ccgccaatct
     1261 cgtggatgtc gccgttcagc gtcatgccca tgtcgggata gtgttcgaac accaccccct
     1321 ttgtcctcag cgcctgaacg gtttcgacca gatcgccgtc catgtcccaa ctgacggcgt
     1381 tggccttgtt cgtgccggcg aagtcggatt tgtagaccgt cagccgggtg tcgccggtgc
     1441 ggaagcccag cacgccgcct tcggtgtccg actgctccag cgtcaggccc agcgtctcct
     1501 catagaaggt tcgggcgcgg tctatgtctc tgacggcgac gatggcggat gagttgcggt
     1561 ccttcagcat ggcggtctcc tgcaaggctt gcgagaccag cgtgcggcgg aggctgcggt
     1621 tccgcttcag cccgcgcagg tctccggcga accggcctcg atccgtccac ccttcagcac
     1681 cagtcgcggc gtgcgtagcg gctccagcgt cgcggtcgga tcgccgtgga cggccaccag
     1741 gtcggcccac gaccgtgcct tcacgacgcc cagaccatcg tccccgagca gacgtccggc
     1801 ctcccacgtc gccgtttgaa gcgcctcggc cgcactcagg ccggcctcga catagccgtc
     1861 catcgtcgcc cgggcgccgg cgccgcggcc ggccccatat gggaagtaga ggtccgagcc
     1921 catggcgat
//
LOCUS       DASVVN010000568         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_310506,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000568 DASVVN010000000
VERSION     DASVVN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310506"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VF686_09210"
     CDS             <1..1584
                     /locus_tag="VF686_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8662035.1"
                     /translation="QLAQLNDQFNPQTLSADSRMSWRLFEYGVQQARLSNRWRDWGYQ
                     FAANGNPTTGLPVFMINNHRVASVSDAEAYVARLRDAERVMGEIAAELRDRASKGVIS
                     PNFVFEPSIANTRNIIAGAPFDGGADNPVWADFQKKVGALEADQATKDRLLGEGRAAL
                     TGEWKRGYEGVLTALAEVQTQAAAMPDRDGGVWRLPDGEAFYNARLQLSTTTDLTADQ
                     IHDTGLSEVARIQAEMEVIKAAVGFTGSLQDFFAFIKTDPRFQYPNTPEGKEQYLTDS
                     RAFIAQVMTDAPRYFHDLPEATLEVRSVEPWREATASVAFYNSPAPDGSRPGIYYVNL
                     SDMTQVLKPQIEAISYHEGAPGHHFQIAYAQEIEGMPRFRRFGGYGAYAEGWGLYAER
                     LGKEMGYYPDPYSDFGRLSLELWRAVRLVTDTGLHAKRWSREQAIEYFQRNSLLSERD
                     IVKEVERYITNPGQATSYKIGELKIRELRAQAEAALGPRFDVRDYHAVVLGSGSVPLE
                     VLEDQVDAWIAAGGGAPAR"
     gene            1666..2004
                     /locus_tag="VF686_09215"
     CDS             1666..2004
                     /locus_tag="VF686_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX8662036.1"
                     /translation="MPLLMCPNDNAAMQTLERGGVQFDMCPSCRGVWLDRGELEKLMA
                     SATEEGRASAPQAAPPPQQAQQPYPPQQPPQQPWGSQPAYRDERYRSRDDDDYRYKKK
                     KRDSIFDIFD"
     gene            complement(2019..3737)
                     /locus_tag="VF686_09220"
     CDS             complement(2019..3737)
                     /locus_tag="VF686_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662037.1"
                     /translation="MAGSVHYEVYIRKTAPAPWALQMATEDRAVAVQTAEDMLADSRA
                     AAVRVTRETLDPDTMEFASSTVLTRGAPEVRHKRVVPESESGPRCNGPQDFYTPQARE
                     LICRVLEDWLARQGVTAFELLHRPDLAEKLEASGVELQHAIQKVAVPESQAVGQPVHD
                     LVRHYQKLADRAVERVIAAGRRGQFPSLENHSLADLAHRLAGQPDRAFIMGGVISCAL
                     TGLRGGRVRLDRLMDLADRAPMDGPPRAMVLVPIEQLLCEMLAARTNLTDILGPALDQ
                     GASLAAVVRMVAPNEIAALIRQDARMALQMPNVDGPAARLGARLEAGEFPLLASALAR
                     MVLRELMSPRRLRPNDAAGEIDILRTLAMALTVTAGRLLTLEEVQNAFNERSKAIVTA
                     DFVAAYAKTCTTVLCEAEALTRLCENVTGTANKRSAARWLSACVGSLRFETEMRANAV
                     GQTAAQKLGVLANLQRAVHGCGLTERDDLEISAAIGTVGGAVEAEARIVMMLARSPAP
                     LPQKLSVLLRMAAGETAPMGPAADRAKVEAIKLFRAPESRAALSAAPEALAPLKGLMR
                     AAGLAA"
     gene            3945..4649
                     /locus_tag="VF686_09225"
     CDS             3945..4649
                     /locus_tag="VF686_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8662038.1"
                     /translation="MANITLVDDDENIVASVSLALESHGHKVTAWHDGAAGLEALEAS
                     PPDLAILDVKMPRMDGMEVLRRLRQTSQIPVIMLTSRDEEIDEILGFNLGADDYIHKP
                     FSQRLLIERVKALLRRTGADGGEPEPSGENSSKAIKRGKLSMDPARHESTWEGKPVKL
                     TVTEFLLLQALAQRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHVKRMRKKFRVVDPEF
                     DAIETLYGVGYRYRES"
     gene            4618..>4988
                     /locus_tag="VF686_09230"
     CDS             4618..>4988
                     /locus_tag="VF686_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor N-terminal transmembrane
                     domain-containing protein"
                     /protein_id="HEX8662039.1"
                     /translation="MALDTAIAKADPLPPGDDEPRRPLKRFGGSRLGGLILALNLLSL
                     LILFVGALLLNEWQRGLIEARQETLIAQAELLANVLADKEYTVGEPNPQFNVEKAPLL
                     LRDNFIPAGQRARLFDLDGLLV"
BASE COUNT          816 a         1703 c         1701 g          768 t
ORIGIN      
        1 cagctggcgc aactgaacga ccagttcaat ccgcagaccc tcagcgccga cagccggatg
       61 tcgtggcggc tgttcgaata cggcgtgcaa caggcgcggc tgtccaaccg ctggcgcgac
      121 tggggctatc agttcgccgc caacggcaat ccgacgaccg gcctgccggt attcatgatc
      181 aacaaccacc gcgtcgccag cgtctccgac gccgaggcct atgtggcccg cctgcgcgac
      241 gccgagcggg tcatgggcga gatcgccgcc gaactgcgcg accgggcttc aaagggcgtc
      301 atctcgccga atttcgtgtt cgagccctcg atcgccaaca cccggaacat cattgccggc
      361 gcgcccttcg acggcggcgc cgacaatccg gtctgggccg atttccagaa gaaggtcggg
      421 gctcttgagg ccgatcaggc gaccaaggac cggctgctgg gcgagggccg cgcggccctg
      481 accggcgagt ggaagcgcgg ttatgagggc gttctgacgg cgctggccga ggtgcagacg
      541 caggcggcgg cgatgcccga ccgcgacggc ggcgtctggc ggctgccgga cggcgaggcc
      601 ttctacaacg cccggctcca gctctcgacc accaccgacc tgacggcgga ccagatccac
      661 gacaccggcc tgtccgaggt ggctcgcatc caggcggaga tggaagtcat caaggccgcg
      721 gtcggcttca ccggctcgct gcaggacttc ttcgccttca tcaagacgga cccgcggttc
      781 cagtatccga acacgccgga gggcaaggag cagtatctga ccgactcccg cgccttcatc
      841 gcgcaggtga tgacggacgc ccctcggtat ttccacgacc tgcccgaggc gacgctggag
      901 gtgcggtcgg tcgagccgtg gcgcgaggcg acggcctcgg tcgccttcta caactcgccg
      961 gcccccgacg ggtcgcggcc cggcatctat tacgtcaacc tgtccgacat gacccaggtg
     1021 ctgaagcccc agatcgaggc catcagctat cacgagggcg cgccgggcca ccatttccag
     1081 atcgcctacg cgcaggagat cgagggcatg ccgcgcttcc ggcgcttcgg tggctatggc
     1141 gcctatgccg agggctgggg cctgtatgcc gagcggctgg gcaaggagat gggctattat
     1201 ccggacccgt attcggactt cggccgcctg tcgctggagc tgtggcgggc ggtgcggctg
     1261 gtcaccgaca cgggcctgca cgccaaacgc tggagccggg aacaggccat cgaatatttc
     1321 cagcgcaact cgctgctcag cgagcgcgac atcgtcaagg aggtcgagcg ctacatcacc
     1381 aacccggggc aggcgaccag ctacaagatc ggcgagctga agatccgcga attgcgcgcc
     1441 caggccgagg cggcgctcgg gccgcggttc gacgtgcgcg attatcacgc cgtggttctg
     1501 ggttccggct cggtaccgct ggaggtgctg gaggatcagg tcgacgcctg gatcgcggcg
     1561 ggcggaggcg cgccggcgcg ctgagacggc gcggcccttg gtcgcgttgg tggaacctcg
     1621 aacccgccct agctctacgg tctggaaccg tgaatggaga cgcctatgcc gctcttgatg
     1681 tgcccgaacg acaacgccgc catgcagacg ctggaacgtg gcggggtgca gttcgacatg
     1741 tgccccagct gccgcggcgt ctggctggat cgcggcgaac tggagaagct gatggcgtcc
     1801 gcgaccgagg agggccgcgc ctccgcaccc caggccgcgc ctccgccgca acaggctcaa
     1861 cagccgtacc cgccgcagca gccgccccag cagccgtggg gttcgcagcc cgcctatcgc
     1921 gacgagcgct accgcagccg cgatgacgac gactaccggt acaagaagaa gaagcgcgac
     1981 agcatcttcg acatcttcga ctgaggtcgt cgaaggacct aggccgcaag gccggcggcc
     2041 ctcatcagcc ccttgagcgg ggcgagggcc tccggcgccg ccgacagggc ggcgcgggat
     2101 tccggcgcac ggaacagctt gatcgcctcg accttggccc ggtccgcggc cggtcccatc
     2161 ggcgccgtct cgccggcggc catacgcagc aacaccgaca gcttctgcgg caggggcgcg
     2221 ggcgagcggg ccagcatcat cacgatccgg gcctcggcct cgaccgcccc gccgaccgtg
     2281 ccgatggcgg ccgaaatctc caggtcgtcg cgttcggtca ggccgcagcc gtgcacggcg
     2341 cgctgcagat tggccagcac gcccagcttc tgggcggcgg tctggccgac ggcgttggcg
     2401 cgcatctcgg tctcgaaccg cagagatccg acgcaggccg acagccagcg ggccgccgaa
     2461 cgcttgttgg ccgtgccggt gacgttctcg catagtcggg tcagcgcttc ggcctcgcac
     2521 agcaccgtgg tgcaggtctt ggcgtaggcg gcgacgaaat cggcggtgac gatggccttg
     2581 gagcgctcgt tgaacgcgtt ctggacctct tccagcgtca gcaggcggcc tgcggtgacc
     2641 gtcagcgcca tggccaaggt gcgcaggatg tcgatctcgc cggcggcgtc gttggggcgc
     2701 agtcgccgcg ggctcatcag ttcgcgcagc accatccgag cgagggccga ggccagcagg
     2761 gggaattcgc ccgcttcgag ccgcgcgccc agccgggcgg cgggaccgtc gacattgggc
     2821 atctgcagcg ccatccgggc gtcctgccgg atcagggcgg cgatctcgtt gggggccacc
     2881 atccgcacca cggcggccag cgaggctccc tgatccagcg cgggcccgag gatgtcggtc
     2941 agattggtgc gcgccgccag catttcgcac agcagctgtt cgatcggcac cagcaccatg
     3001 gcccgcggcg ggccgtccat cggggcccgg tcggccagat ccatcagccg gtccagcctc
     3061 actcggccgc cccgcagccc ggtcagggcg cacgaaatga ctccgcccat gatgaaggcg
     3121 cggtcgggct gacccgccag ccggtgggcc aggtcggcga gcgaatggtt ttccaggctg
     3181 gggaactggc cgcgtcgccc tgccgcgatg acccgttcga cggccctgtc agccagtttc
     3241 tgatagtgac ggaccagatc gtggaccggc tggcccacgg cctggctttc gggcacggcc
     3301 accttctgga tggcgtgctg cagctcgacg cccgaagctt ccagcttttc ggccaggtcc
     3361 gggcggtgca gcagttcgaa ggcggtcacg ccctgacgcg ccagccagtc ctccagcacc
     3421 cggcagatca gctcgcgggc ctggggcgtg tagaagtcct gcggtccgtt gcagcggggc
     3481 ccgctctcgg actccggcac cacccgcttg tgccgcacct cgggcgcgcc gcgggtcagg
     3541 accgtcgaac tggcgaactc catggtgtcg ggatcgaggg tttccctggt cacccgcacg
     3601 gccgcggcgc ggctgtcggc cagcatgtcc tcggccgtct ggaccgccac ggcgcggtcc
     3661 tccgtcgcca tctggagcgc ccagggtgcg ggggccgtct tccggatgta aacttcgtag
     3721 tggacggagc cggccatcaa aacgctctaa ctgttcccgc ccatgatggc gccaaggctt
     3781 taaggcgggg tttcgttgtc aattgtggcg atgcggccca tctaggggcc ttgccgtccg
     3841 ggttttcggt cgaccggccc cggacaatga tggattagaa cggtaacgct tgccgtgcgt
     3901 tgagactgcg aacgacgaac gaagagcctg caaggagacc gcctttggcc aacatcaccc
     3961 tggtcgacga cgacgagaac atcgtcgcct ccgtctcgct ggcgctggaa agccacggcc
     4021 acaaggtcac tgcctggcac gacggggccg ccgggcttga ggcgctggag gccagtccgc
     4081 cggatctggc cattctggac gtcaagatgc cccgcatgga cgggatggag gtgctgcgcc
     4141 gcctgcgcca gacctcgcag attccggtca tcatgctgac ctcgcgcgac gaggagatcg
     4201 acgagatcct gggattcaac ctcggcgccg acgactacat ccacaaaccc ttcagccagc
     4261 gtctgctgat cgaacgcgtc aaggccctgc tacgccggac cggggccgac ggcggcgagc
     4321 ccgagccatc gggcgagaat tcctccaagg ccatcaagcg cggcaagctg tcgatggacc
     4381 cggcccggca cgagagcacc tgggagggca agccggtcaa gctgaccgtc accgaattcc
     4441 tgctgctgca ggcgttggcc cagcgccccg gtttcgtgaa gagccgcgac aacctgatgg
     4501 acgccgccta cgacgatcag gtctatgtcg acgaccgcac catcgacagc cacgtcaagc
     4561 ggatgcggaa gaagttccgg gttgtcgacc ccgagttcga cgccatcgag accctgtatg
     4621 gcgttggata ccgctatcgc gaaagctgat ccgctcccgc cgggagacga cgagccgcgc
     4681 cgcccgctga aacgcttcgg ggggtcgcgg ctcggcggcc tgatcctggc cctgaacctg
     4741 ctcagcctgc tgatcctgtt cgtcggggcg ctgctcctga acgaatggca gcgcgggttg
     4801 atcgaggcgc ggcaggagac gctgatcgcc caggccgagc tgctggccaa tgtgctggcg
     4861 gacaaggaat acacggtcgg cgagcccaat ccgcagttca acgtcgaaaa ggcgcctctg
     4921 ctgttgcggg acaatttcat tcccgccggc cagcgcgccc ggctgttcga cctcgacggc
     4981 ctgctcgt
//
LOCUS       DASVVN010000569         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_338068,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000569 DASVVN010000000
VERSION     DASVVN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338068"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF686_09235"
     CDS             complement(<1..390)
                     /locus_tag="VF686_09235"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268747.1"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX8662040.1"
                     /translation="MAPLAPGSTLGILGGGQLGRMLSQAASRLGFNVVILDPEENSPA
                     GRVSQGQIVAAYDDPTAQMVLGQTCQAVTFEFENVPATSVQRLVRGGAVVAPGPKALA
                     VAQDRVDEKTFLNGVGATTVDFAAVDNL"
     gene            complement(391..879)
                     /gene="purE"
                     /locus_tag="VF686_09240"
     CDS             complement(391..879)
                     /gene="purE"
                     /locus_tag="VF686_09240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX8662041.1"
                     /translation="MPTQTPVAIIMGSRSDWPTMKHAADALDRLGVAWDAKVVSAHRT
                     PQRLFDFATGAKAAGFKVIIAGAGGAAHLPGMAASMTELPVLGVPVESKALKGLDSLL
                     SIVQMPGGIPVATLAVGEAGAKNAGILAAQILALADPVLAGRLTAFREAQTEAVHETV
                     ED"
     gene            1005..1568
                     /locus_tag="VF686_09245"
     CDS             1005..1568
                     /locus_tag="VF686_09245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8662042.1"
                     /translation="MTNAAVIDPYSEIERLRAEIDALRRRAEAAEAAADHDVLTPVLN
                     RRGFIAQMKSAMAFCQRHGTPAVLLYLDLDGFKGVNDSLGHAAGDAALVHIANLLTAN
                     LRESDAVGRLGGDEFGLLMLNAGLNGGREKAQRLAAALEAEEFTWNGLSARLGGSFGV
                     RAFNGHTDPEVWLAEADAAMWVRKKGR"
     gene            1565..1879
                     /locus_tag="VF686_09250"
     CDS             1565..1879
                     /locus_tag="VF686_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662043.1"
                     /translation="MTDWLEAETDWVGRRVLVGLTFLHPDESFDRQEQMFGRIVSMDR
                     SNGVEVALEGQRVGETFWLPPDLRPFSEAAPGEYRLRSTGETVVDPDLLATWTITAPA
                     RT"
     gene            complement(1876..2100)
                     /locus_tag="VF686_09255"
     CDS             complement(1876..2100)
                     /locus_tag="VF686_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8662044.1"
                     /translation="MNDDEPFLTEEEQALHARLKLLQLEHADLDASIEALGRMPVPDQ
                     LMIARLKRKKLLLRDEITKIEDRILPDIIA"
BASE COUNT          318 a          689 c          777 g          338 t
ORIGIN      
        1 gaggttgtcc acggcggcga agtcgaccgt ggtcgcgccg acgccgttga ggaaggtctt
       61 ttcgtcgacc cgatcctgcg ccacggcgag ggccttgggg ccgggggcga cgacggcgcc
      121 gccccgaacc agccgctgga ccgaggtcgc ggggacgttc tcgaactcga aggtgacggc
      181 ctgacaggtc tggcccagca ccatctgggc ggtgggatcg tcataggcgg cgacgatctg
      241 gccctgcgac acgcgcccgg cggggctgtt ttcctcggga tcgaggatga cgacgttgaa
      301 gccgaggcgg gacgcggcct ggctgagcat gcggcccagc tggccgccgc cgagaatgcc
      361 gagcgtcgag ccgggagcca gaggagccac tcagtcctcg accgtctcgt ggaccgcctc
      421 ggtctgggcc tcgcggaagg cggtcagacg tccggccagc accggatcgg ccagggccag
      481 gatctgggcc gcgaggatgc cggcgttctt ggcccccgcc tcgccgacgg cgagggtggc
      541 gacgggaatg ccgcccggca tctggacgat ggacagcagg ctatccagcc ccttcagcgc
      601 cttggactcg accggcacgc ctagcaccgg cagctcggtc atggaggcgg ccatgcccgg
      661 aaggtgggct gcgccgccgg cgccggcgat gatgaccttg aagcccgcgg ccttcgcgcc
      721 cgtcgcgaag tcgaacaggc gctgcggcgt gcggtgggcg ctgacgacct tcgcatccca
      781 tgcgacaccg aggcggtcca gcgcgtcggc ggcgtgtttc atggtgggcc agtccgagcg
      841 gctgcccatg atgatcgcca cgggtgtctg ggtcggcatg ggtgcatgct ctttcgcggg
      901 aaagcggcgc gatacaggac tcgcgttgcg cccgcaaccg acggcgttag gctggccctt
      961 ggccgcgcgt ggtgagtcgc ggggccgggg agccttcttc gctcgtgacc aacgcggccg
     1021 tcatcgaccc ttacagcgag atcgagcgcc tgcgcgccga gatcgacgcc ctgcgccgcc
     1081 gcgccgaagc ggccgaggcg gcggcggatc acgacgtgct gacgccggtg ctgaatcggc
     1141 gcggtttcat cgcccagatg aagagcgcca tggccttctg ccagcgccac ggcacgccgg
     1201 cggtgctgct ctacctcgat ctggacgggt tcaagggcgt caacgacagc ctcggccacg
     1261 ccgccggcga cgcggcgctg gtccacatcg ccaatctgct gacggcaaac ttacgcgaat
     1321 cggacgccgt gggccgtctg ggcggcgacg agttcggatt gctgatgctg aacgcgggtc
     1381 tgaacggcgg ccgcgagaaa gcccagcggc tggcggccgc gctcgaggcc gaggaattca
     1441 cctggaacgg cctgtccgcg cgtctgggcg gatcgttcgg ggtgcgcgcc ttcaacggcc
     1501 acaccgatcc cgaggtctgg ctggccgagg ccgacgccgc catgtgggtg cggaagaagg
     1561 gccggtgacc gactggctcg aagcggaaac cgactgggtc ggccggcgtg tgctggtggg
     1621 gctgacattc cttcatccgg acgagtcgtt tgatcggcag gagcagatgt tcggacggat
     1681 cgtctcgatg gaccgcagca atggtgtcga ggtcgccctt gagggacaga gggtcggaga
     1741 gacgttctgg ctcccgcccg atctgcgtcc gttctccgag gccgcgcccg gagaataccg
     1801 actgcgttct accggtgaaa cggtggtcga ccccgacctg ctggcgacgt ggacgatcac
     1861 ggctcctgcg cgcacctagg cgatgatgtc gggcaggatc cggtcttcga tcttggtgat
     1921 ctcgtcgcgc agcagcagct tcttccgctt cagccgggcg atcatcagct gatcgggcac
     1981 aggcatgcgc ccgagggcct cgatcgaggc gtcgaggtcg gcgtgctcga gctgaagcag
     2041 cttcagccgg gcgtggagcg cctgctcttc ctcggtcaga aacggttcgt cgtcattcat
     2101 actcgcaccc cggacgcggt ta
//
LOCUS       DASVVN010000570         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_393708,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000570 DASVVN010000000
VERSION     DASVVN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393708"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF686_09260"
     CDS             <1..590
                     /locus_tag="VF686_09260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX8662045.1"
                     /translation="LTGGDTSERSILIMLVANLLYIYLDFSAYCDLAIGAARAGGRRL
                     PENFDWPLVRSGLRRYWRHWHMTLSQWVMRRVYFPAFLASHSTALALFASMLTIGLWH
                     APTISWSFWALHHSLAMAVEARVSPGSPPESPRPQGPVARVRTGARHLVGMGFMLAWV
                     SLGHSFTLFAAPRLALETYGRALAAPFVIVWRMFA"
     gene            745..>1776
                     /locus_tag="VF686_09265"
     CDS             745..>1776
                     /locus_tag="VF686_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8662046.1"
                     /translation="MTKAGYETARVSARETPDTFWSEQARRLDWMTAPTHIKDVSFDK
                     TDFRIRWFDDGVLNVAWNCLDRHLAERGDQTAILWEGDEPTQSGGLTYRELRAEVCRM
                     ANVLKGLGVVKGDRVTLYMPMIPAAAVAMLACARIGAVHSVVFGGFSPDSLAGRIEDC
                     GSRVVITADEGLRGGKRVPLKANVDAALEKLPAGSRVDKVLIVSHTNADMPIMAGRDV
                     RYGEAKHGVSDDCPCEPMNAEDPLFILYTSGSTGKPKGVLHTTGGYLFWAAWTHELVF
                     DYRPGEVFWCTADVGWVTGHSYCVYGPLANGATTLMFEGVPNYPDHSRFWRVIDKHRV
                     EIFYTAPTAL"
BASE COUNT          278 a          586 c          587 g          325 t
ORIGIN      
        1 atcttacagg cggcgatacc tcggagcggt caattctgat catgctcgtc gccaacctgc
       61 tctacatcta tctcgatttt tcagcctatt gcgacctcgc catcggggcg gcccgtgccg
      121 gggggcggcg gctgcccgag aattttgatt ggccgttggt tcggagcgga ctgcgccggt
      181 actggcggca ctggcacatg accctttcgc agtgggtcat gcggcgggtc tatttcccgg
      241 ccttcctggc ttcccactcg acagccctgg ccctgttcgc gtcgatgctg acgatcggtc
      301 tttggcacgc gcccacgatc tcgtggagtt tctgggccct gcaccacagc ctcgcgatgg
      361 cggtcgaggc tcgcgtcagt cccggctccc ccccggaatc gccgcgccct caggggccag
      421 ttgcgcgggt taggacgggc gcccgtcatc tggtcggcat gggattcatg ctggcctggg
      481 tctccttggg ccacagcttc acactgttcg cagcaccgcg tttggcgctt gagacctatg
      541 gccgggcgct ggcggcgcct ttcgtcatcg tctggcgaat gttcgcctga gggagaccgg
      601 ctggaaccgg agcgctggac gacgccgcag tcatgctgtt cgatatcggc cgactggtag
      661 cgactatcaa ccgcttgaaa gacgacgccc ttgaccgacc ccgagctcta tcctgttccc
      721 tcggagtggg cccaacgcgc acggatgacg aaggcgggat acgagaccgc gcgagtgtcg
      781 gcgcgcgaga cgcccgacac cttctggagc gagcaggccc gccggctgga ctggatgacc
      841 gcgccgaccc acatcaagga cgtgtcattc gacaagaccg acttccgcat ccgctggttc
      901 gatgacgggg tcctgaacgt cgcgtggaac tgtctcgacc ggcatctggc cgaacgcggc
      961 gaccagaccg cgatcctctg ggagggcgac gagccgacgc agtctggcgg cctgacctat
     1021 cgcgaactgc gcgccgaagt ctgccgcatg gccaatgtgt tgaagggcct cggcgtcgtg
     1081 aagggcgacc gggtcaccct ctatatgccc atgatcccgg cagcggccgt tgcgatgctg
     1141 gcctgcgccc ggatcggggc ggtccattcg gtcgtcttcg gcggcttctc gcctgacagt
     1201 ctggccggcc ggatcgagga ctgcggatca agggtggtga tcaccgccga cgagggcctg
     1261 cgtggcggca agcgcgtgcc gttgaaggcg aacgtggatg cggcgctgga gaaactcccg
     1321 gccggctccc gggtcgacaa ggtgctgatc gtctcgcaca ccaacgccga catgccgatc
     1381 atggccggcc gcgacgtccg ctacggcgag gccaaacacg gcgtcagcga cgactgccca
     1441 tgcgaaccga tgaacgcgga ggacccgctg ttcatccttt acacctcggg ctcgaccggg
     1501 aagcccaagg gcgtgctgca caccacaggc ggctatctgt tctgggcggc gtggacgcac
     1561 gagctggtgt tcgactaccg ccccggcgag gtcttctggt gcaccgccga cgtgggctgg
     1621 gtcacgggcc acagctattg cgtctatggc ccgctggcca atggcgcgac caccctgatg
     1681 ttcgagggcg tgcccaacta tccggatcac agccgcttct ggcgggtgat cgacaagcac
     1741 cgggtcgaga tcttctacac cgcgcccacg gcgctg
//
LOCUS       DASVVN010000571         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_181438,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000571 DASVVN010000000
VERSION     DASVVN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181438"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..581)
                     /locus_tag="VF686_09270"
     CDS             complement(144..581)
                     /locus_tag="VF686_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662047.1"
                     /translation="MNYVQGMGVPEFHELFPHSPAFRDMQPLRIPAGWRIVWNQLDIG
                     MATDVTGVGGSSVFHATNESRRFNIDVEFRPEFDPDGSFHLTIVYQPWPRTERGRRRQ
                     DVAFAFDGDGQTVHTFETRAYPDLVAELEHWIARCTVWEREGH"
     gene            638..799
                     /locus_tag="VF686_09275"
     CDS             638..799
                     /locus_tag="VF686_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662048.1"
                     /translation="MADPRDTDPIDQDPRERGLERRSDAPAVSPWLVIGAILMLGAGV
                     YVISAMVMR"
     gene            846..>1820
                     /gene="purD"
                     /locus_tag="VF686_09280"
     CDS             846..>1820
                     /gene="purD"
                     /locus_tag="VF686_09280"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX8662049.1"
                     /translation="MNILLVGSGGREHALAWKIAQSPLLRRLVIAPGNPGMEKLGELR
                     PMKVTDADGLCALAREMKADLVVVGPETALEAGLADRLAAAGIPCFGPTKKAAQLETS
                     KAFSKAFMERHEIPTAGYGVYERLHEARQALTVFKPPYVIKADGLAAGKGVAISPNKR
                     EAEAEIERMLGGRFGTAGARVVIEEFMAGEEGSLFAVCDGQRAVLLGGAQDHKRAYDG
                     DLGPNTGGMGSYSPAPVFTPELVEAADRLVVQPVVAAMAAEGAPYRGVLYAGLMATED
                     GPKVVEFNARFGDPECQVLMMRLDGDVAPLLLACARGDLTKAPMPAFRD"
BASE COUNT          283 a          573 c          690 g          274 t
ORIGIN      
        1 cgcggtcgcc tgaaacaggg cgccgtgatg cttgcggcag tcgaggcagt gacagaggcc
       61 cacccggtat gggcgtccgg tcgccacgac gcggacgtcg ccgcacaggc agccgccggt
      121 gaattggtcc atgtcgggtc tccctagtgg ccttcccgct cccatacggt gcagcgagcg
      181 atccagtgtt ccagttccgc gacgagatcg ggataggcgc gcgtctcgaa cgtgtggacg
      241 gtctgcccgt cgccatcgaa ggcgaaggcg acgtcctgac ggcgacggcc gcgctcggtc
      301 cggggccagg gctggtagac gatggtgagg tggaacgagc cgtcgggatc gaactcgggg
      361 cggaactcga catcgatgtt gaagcggcgg ctctcgttcg tagcatggaa cactgaggag
      421 ccgcccacgc cggtaacatc ggtcgccatc ccgatgtcga gctggttcca gacgatccgc
      481 cagccggcgg ggatgcgtag cggctgcatg tcccgaaagg ccggggagtg ggggaaaagc
      541 tcgtggaatt caggtacgcc catgccttga acgtagttca aaaagcgccg cctgcaacgg
      601 cccggaaccc ctcccatggt cccgccgttg cccctgcatg gccgaccccc gcgacaccga
      661 ccccatcgat caggaccccc gggagcgcgg tctggagcgg cgcagcgacg cgccggcggt
      721 cagtccgtgg ctggtcatcg gggcgatcct gatgctgggc gcgggggtct atgtgatctc
      781 ggcgatggtg atgcggtaac catgcgcgac ttgaccggcc tcgcaggtgc ggtcattgcc
      841 ccggcatgaa tatcctgctg gtcggatcgg gcgggcgaga gcacgccctg gcgtggaaga
      901 tcgcccagtc gccgctgttg cggcgtctgg tcatcgcgcc cggcaatccg ggcatggaga
      961 agctgggcga gctgcggccc atgaaggtca cggacgccga cggcctgtgc gccctggcgc
     1021 gcgagatgaa ggccgatctg gtggtggtcg ggcccgagac cgcgctggag gccgggctgg
     1081 ccgaccggct ggccgccgcg ggcattccct gtttcgggcc gacgaagaag gccgcgcagc
     1141 tggaaacgtc caaggccttc tccaaggcct tcatggagcg gcacgagatt ccgacggccg
     1201 gatacggcgt ctatgagcgg ctgcacgagg cgcggcaggc gctgacggtg ttcaagcccc
     1261 cctatgtgat caaggccgac ggtctggccg ccggcaaggg cgtcgccatc agccccaaca
     1321 agcgcgaggc cgaggccgag atcgaacgga tgctgggcgg ccggttcgga accgccggag
     1381 cgcgggtggt gatcgaggag ttcatggccg gcgaggaggg ctcgctgttc gccgtctgcg
     1441 acgggcagcg ggcggtgctg ctgggcgggg cgcaagacca caagcgcgcc tacgacggcg
     1501 atctgggccc gaacaccggc ggcatgggca gctattcgcc ggcgccggtg ttcacgccgg
     1561 aactggtcga ggcggcggat cggctggtcg tccagccggt ggtcgcggcg atggcggccg
     1621 agggcgcgcc ctatcgcggg gtgctgtacg ccgggctgat ggcgaccgag gacggcccga
     1681 aggtggtcga gttcaacgcc cgtttcggcg atccggaatg ccaggtgctg atgatgcggc
     1741 tggacgggga cgtcgcgccc ctgttgctgg cctgcgcgcg gggcgacctg acgaaggcgc
     1801 cgatgccggc cttccgcgac
//
LOCUS       DASVVN010000572         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_100059,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000572 DASVVN010000000
VERSION     DASVVN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100059"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1085)
                     /locus_tag="VF686_09285"
     CDS             complement(147..1085)
                     /locus_tag="VF686_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8662050.1"
                     /translation="MERDAHYAAVGIATIALLAALAVFTIWLARLQFNNDYDVYDIVF
                     YGPVRGLSEGGEVHFNGIRVGEVTDLNLDPKKGDQVIARIRVDGTTPVRVTSRAQLEP
                     QGITGLNYIQITAGNPQSAILKTQYPDNVVPVIQSQPSPIAELLSGSGTVLAQTVDAL
                     NRINRVLSDDNIRSFSTSVKNVEALSSELEARKGMFAQLEQALTKANAAIGEYQALGV
                     SARQLVEGDGRQAIANINRAADEAAEAAASANRTISGLEQPVNDFATTGLPQLQQSIQ
                     GLEDATQALEGLIEEVRRSPRDFIGRAPTKEMEVQP"
     gene            complement(1099..1869)
                     /locus_tag="VF686_09290"
     CDS             complement(1099..1869)
                     /locus_tag="VF686_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8662051.1"
                     /translation="MAKPERLIEVRGLLSQFGERTIHENLDLDLERGEVLGVVGGSGT
                     GKTVLLNSIIGLKEPEGGSVSILGYDRATMTQDQEADVERRTGILFQQGALFSSLSVI
                     DNVASPLVEHTDLSKEVIRELAEMKIAMVGLKPEAHHLKPAELSGGMRKRVGLARALA
                     LDPELLFLDEPTAGLDPIGAAAFDDLIKQLSNDLGLTVFMITHDLDSLYAITDKVAVL
                     ADKHVVAKATVQELERSDHPWIREYFLGPRGRAADKAA"
     gene            complement(1896..>2471)
                     /locus_tag="VF686_09295"
     CDS             complement(1896..>2471)
                     /locus_tag="VF686_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8662052.1"
                     /translation="FLGANMLTQFGAAVFTVQLVAVSVLREFAVVITSVLLAGRSSSS
                     FAAEIGSMRMNQEVDAMQVMGVNPFQALVIPRLTAMVLMLPLLTFIGMVAGLFGGLLV
                     TWSQLSYGPSFFVQRISEDPLMGQHLMVGMIKAPVFAIVIAAIGCRQGMAVAGDVESL
                     GRRVTAAVVQAIFAIILLDAVFALIFLELNL"
BASE COUNT          417 a          817 c          812 g          425 t
ORIGIN      
        1 cgaggagccg atgctctccg acaccgcggg cgccggcccg ccgaagcggt agctctggac
       61 aggatccggc gagctcaaca gggcgcaacc cgaaagtacg gaggcgagcc cgccgacggc
      121 ggctagccgg ataagggttg aacggatcac ggctggacct ccatttcctt ggtcggcgcg
      181 cggccgatga agtcgcgcgg gctgcgccgc acctcctcga tcaggccttc gagagcctgc
      241 gtggcgtctt ccagtccctg gatggactgc tgcagctggg gcaggccggt ggtggcgaag
      301 tcgttcacgg gctgctccag accgctgatc gttcgattgg ccgaggccgc agcctccgcc
      361 gcctcgtccg cggcccggtt gatgttggcg atggcctgac gaccatcccc ctcgaccagc
      421 tgccgggcgc tgacgcccag cgcctggtat tcgccgattg cggcgttggc cttggtcagc
      481 gcctgttcca gctgggcgaa catccccttg cgggcttcca gctcgctgga cagggcctcg
      541 acgttcttca cgctggtcga gaacgagcgg atgttgtcgt ccgacagcac ccggttgatc
      601 cggttcagcg cgtccaccgt ctgcgccagc accgtgcccg agccgctcag cagttcggcg
      661 atcggcgaag gctgactctg gatcacgggc acgacattgt cagggtactg ggtcttcagg
      721 atcgcgctct gcggattgcc ggcggtgatc tggatgtagt tcaggccggt gatgccctgc
      781 ggctccagct gggcgcgcga ggtcacgcgc accggcgtgg tgccgtctac gcggatacgg
      841 gcgatgacct gatcgccctt cttcggatcg aggttcagat cggtcacctc gccgacacgg
      901 atgccgttga agtgcacctc tccgccctcc gacagcccgc gcacgggtcc gtagaagaca
      961 atgtcataga cgtcgtagtc gttgttgaac tgcagccggg ccagccagat ggtgaacacc
     1021 gccagcgccg ccagaagggc gatagtggcg atgccgacgg cggcgtagtg ggcgtctctt
     1081 tccatctttc ggctctcctt aagccgcctt gtccgcggcg cgaccgcgcg gaccaaggaa
     1141 gtattctcgt atccaggggt ggtcggaacg ttcgagttcc tgaaccgtgg ccttggcgac
     1201 gacgtgtttg tcagccagca ccgcgacctt gtcggtaatg gcgtacaggc tgtcgaggtc
     1261 gtgggtgatc atgaagacgg tcaggcccag atcgttcgac agctgtttga tcaggtcgtc
     1321 gaacgccgcc gcgccgatcg ggtccagccc cgccgtcggc tcgtcgagga acagcagttc
     1381 cggatccagc gccagcgccc gcgccagccc gacgcgcttg cgcatgccgc ccgaaagctc
     1441 ggccggtttc agatggtgcg cctcgggctt cagcccgacc atggcgatct tcatctcggc
     1501 cagttcgcgg atcacctcct tcgacaggtc ggtatgctcg accagcggcg aggcgacgtt
     1561 gtcgatgacg ctgagcgacg agaacagggc gccctgctgg aacagaatgc cggtgcgccg
     1621 ttcgacatcc gcctcctgat cctgggtcat ggtggcgcgg tcgtagccga ggatgctcac
     1681 agacccgccc tcgggttcct tcaaaccgat gatggagttc agcaacacgg tcttgccggt
     1741 gcccgagccg ccgaccacgc ccagaacctc gccccgctcc agatcgagat cgaggttctc
     1801 gtggatggtg cgttcgccga actggctcag caggccgcgc acctcgatca ggcgttcggg
     1861 cttcgccatc gggtcggttt tgagatccgc ggccgtcaca ggttcagctc caggaagatc
     1921 agggcgaaga cggcgtccag caggatgatg gcgaagatgg cctgaaccac cgccgccgtg
     1981 acccgccggc cgaggctttc cacatcgccc gccacggcca tgccctgccg gcagccgatg
     2041 gcggcgatga cgatggcgaa gaccggggcc ttgatcatgc cgaccatcag gtgctgaccc
     2101 atcaacggat cctcgctgat cctctggacg aagaaggacg ggccgtagct gagctggctc
     2161 cacgtcacca gcaggccgcc gaacagtccg gccaccatgc cgatgaaggt cagcagcggc
     2221 agcatgagga ccatggccgt cagccgtggg atgacgagcg cctggaacgg attgacgccc
     2281 atgacctgca tggcgtccac ctcctggttc atccgcatcg aaccgatctc ggccgcgaag
     2341 gatgaggagg accggccggc cagcagcacc gaggtgatga ccaccgcgaa ctcgcgcagc
     2401 accgagaccg ccaccagttg caccgtgaac acggccgccc cgaactgggt cagcatgttc
     2461 gcgccgagaa a
//
LOCUS       DASVVN010000573         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_22362, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000573 DASVVN010000000
VERSION     DASVVN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22362"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF686_09300"
     CDS             <1..393
                     /locus_tag="VF686_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX8662053.1"
                     /translation="EPPAAESIGDLDVSAAEPEPFPSAPVSESVFAAEPVEVHTPENP
                     AVPTTNYDRLVGDSAAASAASAFAGFASTLKKPEPADIEFPSGPTIDEMVRGLLRPML
                     KEWLDANLPAIVETQVRKEVERIARTAG"
     gene            481..>1682
                     /locus_tag="VF686_09305"
     CDS             481..>1682
                     /locus_tag="VF686_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8662054.1"
                     /translation="MLDKTFDPTAAEPRIYRQWEDSGLFAPKTDGAAEAYSIVIPPPN
                     VTGSLHIGHALNNTLQDILARYHRMKGKAVLWLPGTDHAGIATQMVVERQLAAAGNIG
                     RRDMGRDAFVAKVWEWKAESGGTIVQQLRRLGASCDWSRERFTLDEGLNAAVRKVFVQ
                     LHRDGLIYRDKRLVNWDPHFQTAISDLEVEQREMDGHYWHFAYPLADGVTYEHPIAYD
                     GDGQPTEYETRDYIVVATTRPETMLGDTGVAVHSGDARYEGLVGKFVTLPIVGRRIPI
                     VADDYADPTKGSGAVKITPAHDFNDFQVGKRAGLELINIFDAFGRVLERFDHAQTSIQ
                     IRTSVGHESDSYADMPAGIVDDEPGYGAVPEPFAGMERFAARKAIVARAEEDGWLKEI
                     EKTRHVIP"
BASE COUNT          308 a          581 c          536 g          257 t
ORIGIN      
        1 gagccgccgg ccgccgaaag catcggcgat ctggatgttt ccgccgctga gcccgagccc
       61 ttccccagcg ccccggtcag cgagtccgtt ttcgccgccg agccggtgga ggtccacacg
      121 ccggaaaatc ccgccgtgcc gaccaccaac tatgaccgtc tggtcggcga cagcgccgcg
      181 gccagcgccg cctcggcctt cgccggcttc gcctcgaccc tgaagaagcc cgagccggcc
      241 gatatcgagt tcccgtcggg cccgaccatc gacgaaatgg tccgcggcct gctgcgcccg
      301 atgctgaagg aatggctgga cgccaatctg ccggccatcg tcgagaccca ggtacgcaag
      361 gaagtcgaac gcatcgcccg caccgccggc tagcttcacc tgcgacctga tcaaggctag
      421 atcattccgg ggcggctcca gtcggggccg cccttatctt ttgtctgaag accgttccct
      481 atgctcgaca agaccttcga cccgaccgcc gccgaaccgc gcatctacag acagtgggag
      541 gatagcggcc tgttcgctcc caaaaccgac ggagcggccg aggcctattc gatcgtcatc
      601 ccgccgccga acgtcacggg ctcgctgcac atcggccatg cgctgaacaa caccctgcag
      661 gacatcctgg cccggtatca ccggatgaag gggaaggccg tgttgtggct gccgggcacg
      721 gaccacgccg gcatcgccac ccagatggtg gtcgagcgcc agctggccgc ggcgggcaac
      781 atcggccggc gcgacatggg ccgcgacgcc ttcgtggcca aggtctggga gtggaaggcc
      841 gagagcggcg ggactatcgt ccagcagctg cgccggctgg gcgcatcctg cgactggagc
      901 cgcgagcgct tcaccctaga cgaggggctg aacgcggccg tgcgcaaggt cttcgtccag
      961 ctgcacaggg acggcctgat ctaccgcgac aagcggctgg tgaactggga cccgcatttc
     1021 cagacggcca tctcggacct cgaggtcgag cagcgcgaga tggacggcca ctactggcat
     1081 ttcgcctatc cgctggccga cggcgtcacc tatgagcacc cgatcgcata tgacggcgac
     1141 ggccagccga cggaatacga gacgcgcgac tatatcgtcg tagccacgac gcggcccgag
     1201 accatgctgg gcgacaccgg cgtggcggtg cactccggag acgcgcggta cgaggggctg
     1261 gtcggcaagt tcgtcacgct gcccatcgtc ggtcggcgta tcccgatcgt cgccgacgac
     1321 tatgccgatc cgaccaaggg ttcgggcgcg gtgaagatca cgccggcgca cgacttcaac
     1381 gacttccagg tcggcaagcg tgccggtctc gaactcataa atatcttcga cgcgttcggt
     1441 cgcgtgctcg agcgtttcga tcacgcccaa acctcaatcc agatacggac cagcgtcggg
     1501 catgagagcg attcctacgc cgacatgcct gccgggattg tcgacgacga accgggctac
     1561 ggagccgttc ccgagccttt cgccggcatg gaacgtttcg ccgcgcgcaa ggccatcgtc
     1621 gcccgggccg aggaagacgg ctggctcaag gagattgaga aaacccgcca cgtcatcccg
     1681 ca
//
LOCUS       DASVVN010000574         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_257884,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000574 DASVVN010000000
VERSION     DASVVN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257884"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF686_09310"
     CDS             <1..460
                     /locus_tag="VF686_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX8662055.1"
                     /translation="SISLRRVEFPEAVEGDRLLGVTGTETAYIKGARWVSPAADLYME
                     SLENAFAGQATAVRLVGAREAVRSGRSLDIDMRAFEARYDAPGAVPTVVITARVRVTT
                     APDRTVAAERMFSVEQPATENRVSAIVEAFDMATRDLNTQIVAWTEANAR"
     gene            complement(457..1320)
                     /locus_tag="VF686_09315"
     CDS             complement(457..1320)
                     /locus_tag="VF686_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX8662056.1"
                     /translation="MADGAYRWLGNAPAPDAARVDGIHAALDQTAAHAFGEPARAAAV
                     ERIDRTLTTARADGLEAAVAALSQLRSRIEALRPDALEPRRGLAGLFDSRGKRLNRFR
                     EQFRDAADRLTDSGAALAVRIEGAARRATSLDAVWAEIRDAVADLDAHLAAAARALSG
                     HAPAPAATEDGAVHPLEARKATLDACRAAALQSLPLIRGSQNADARTSEALKSCTDGL
                     AAWRDDWRDALGLSGKRPRRIRPDKARLARSREQALARLDRTLAELTTLGARRGDVER
                     RLAAVAAGVRP"
     gene            1479..2672
                     /locus_tag="VF686_09320"
     CDS             1479..2672
                     /locus_tag="VF686_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8662057.1"
                     /translation="MDATLHRRMVLAGLGYAGLAGCAPTVAVPPTRMRPAFQHPVFAP
                     LLTDPGRLSRITVCTRPFRAQGPRIEVETVGDKRVVHNYGHGGSGWSMAWGSAAAARA
                     LALEGGQRQVAVIGCGALGLTAALQILRAGAEVTIYAAERTPYTRSSRATGVWSPDSR
                     IAAEASMSADFPARWETMARQSWTDHHAFVGLDGDPVRFIDQYNLHGGPGNDGPVGPA
                     PTPGRVGFARLGERISGLAPWSRPLGSDEHPFTVDRATVLPIMTFNVAEYARQLTEAF
                     LMEGGRIVQRTFNHPSELADLAEPVVVNCTGYGARALFGDESVVPVRGQIAWLTPQPE
                     VTYALYYRSISMVPRHDGIVIQANGASQMVGYGVADETPDRAEAEQALAALATLFQPR
                     PGATV"
     gene            complement(2645..3295)
                     /locus_tag="VF686_09325"
     CDS             complement(2645..3295)
                     /locus_tag="VF686_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX8662058.1"
                     /translation="MPRTREQLAERAAKELQDGFYVNLGIGIPTLVANYIPEGMEVTL
                     QSENGMLGMGPFPYEDEVDADLINAGKQTITQIPESSYFSSSDSFAMIRGGHINLSIL
                     GAMEVAENGDIANWMIPGKLVKGMGGAMDLVAGVKRVVVVMDHANKHGQSKVLKSCTL
                     PLTGTGVVSRVITDLCVFDVKPDGSGLELIELAEGVTLDEVASKTEATYTVAPGLG"
     gene            complement(3295..4035)
                     /locus_tag="VF686_09330"
     CDS             complement(3295..4035)
                     /locus_tag="VF686_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX8662059.1"
                     /translation="MRKIFPDAASALEGLTFDGMTVMSGGFGLCGIPEHMIAALRDSG
                     AKGLTVISNNAGVDGFGLGQLLETRQIAKMISSYVGENKEFERQYLAGELQLEFNPQG
                     TLAERIRAGGAGIPAFFTATGVGTLVAEGKEVRTFDGRDYVMETGLVADLSIVKAWKA
                     DERGNLVFRKTARNFNPMMATAGKACVVEVEEIVPVGSLDPDAIHTPGIYVDRIVQTV
                     PEKRIEQRTVRQREPGSSSEAVAQGGKS"
     gene            complement(4259..5089)
                     /locus_tag="VF686_09335"
     CDS             complement(4259..5089)
                     /locus_tag="VF686_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEX8662060.1"
                     /translation="MGDAPHSALYVGEVVHQRVRTFSHRLRYRLWMVLVDLDELDGLQ
                     DRLRLLRRRAFGLITLRTADHGDRSGRPLRLQMERHLANAGIDIAGGPIRLLTMPRIL
                     GYGFNPISVFFCHTPDGALAALLYEVTNTFHERHSYLVAVPSPPPQGERDHAKHGGGG
                     RTAPVRQTAEKQFFVSPFMDQDLTYDFTVRPPGEAVSVVVAVRRGDAPVLTASFAGNR
                     RPLTDGQLLRAFVSHPLLTWKVTWGIHWEAFLGILKGARYRARGAQPARPVTVGHVRF
                     "
BASE COUNT          769 a         1700 c         1942 g          830 t
ORIGIN      
        1 ctcgatctcc ctgcggcggg tcgagttccc cgaggccgtc gagggcgatc gcctgctggg
       61 cgtgaccggc accgagacgg cctacatcaa gggcgcgcgc tgggtgtcgc cggcggcgga
      121 cctgtatatg gaaagcctcg agaacgcctt cgccggccaa gccacggccg tacggctggt
      181 cggcgcgcgc gaggcggtgc ggagcggacg cagcctggac atcgacatgc gcgcctttga
      241 ggcgcgctat gacgcgccgg gcgcggttcc gacggtggtg atcacggccc gggtccgggt
      301 gacgacggcg ccggaccgga ccgtcgcggc cgagcggatg ttcagcgtcg aacagcccgc
      361 gacggagaac cgggtgtcgg cgatcgtcga agcctttgac atggccacgc gggacctgaa
      421 cacccagatc gtggcctgga ccgaggcgaa tgcccgctag ggcctgacgc cggctgcgac
      481 agcggccagc cggcgttcga cgtcgccgcg gcgggcgccc agcgtggtca gttcggccaa
      541 ggtcctgtcg aggcgggcga gcgcctgttc acggctgcgg gcgagacgcg ccttgtccgg
      601 gcggatgcgg cggggacgtt ttccggatag gcccagggcg tcgcgccagt cgtcgcgcca
      661 ggcggccagc ccgtcggtgc aggacttcag cgcctcggac gtgcgggcgt cggcgttctg
      721 tgagccccgg atcagcggca gggactggag ggcggcggcg cggcaggcgt ccagcgtcgc
      781 cttgcgggct tccagcgggt ggacggcgcc gtcctctgtc gcggcggggg ccggggcgtg
      841 gccggacagg gcgcgggcgg cggcggcgag atgggcgtcg aggtcggcga cggcgtcgcg
      901 gatctcggcc cagacggcgt ccagcgatgt ggcgcggcgg gcggctccct caatgcgcac
      961 ggcgagggcc gcgccgctgt cggtcaggcg gtcggcggcg tcgcgaaact gttcgcggaa
     1021 acggttcagg cgcttgcccc ggctgtcgaa cagacccgcg aggccgcggc ggggttcgag
     1081 ggcatcgggc cgaagggcct cgatgcgcga gcggagttgg gacagggccg cgacggcggc
     1141 ctcgaggccg tcggcccggg cggttgtcag ggtccggtcg atgcgttcga cggcggccgc
     1201 acgggcgggt tcgccgaagg catgggcggc tgtctggtcg agcgccgcgt ggatgccgtc
     1261 gacccgcgcc gcgtcgggcg caggggcgtt gcccagccag cgataggctc cgtccgccat
     1321 ctcgatactc cccccagacc ggctctgcgg ccggttaacg ggagtatgcc tacgacttca
     1381 gcccggcggc aacggtgtag ctggccggag cggttgcagg gcggcctcgg attgccgatg
     1441 gtgggcggct caagccattc gccccgggag aacggtcttt ggacgccacc ttgcaccgcc
     1501 ggatggtcct cgccggtctg ggctacgccg ggctcgccgg atgcgccccg accgtcgcag
     1561 tccctccaac acggatgcgg cccgccttcc agcacccggt cttcgcgccc ctgctgaccg
     1621 atcccggtcg gctcagccgc atcactgtct gcaccagacc cttccgggcc caaggccctc
     1681 gcatcgaggt cgagacggtt ggcgacaagc gggtggtgca caactacggc cacggcggct
     1741 ccggttggtc catggcctgg ggttcggccg cggcggcccg cgccttggcg ctcgaaggcg
     1801 gccagcgtca ggtcgcggtg atcggctgcg gcgcgctcgg cctgacggcg gccctgcaga
     1861 tcctgcgggc aggcgccgag gtcaccatct atgcggcgga gcggacgccc tacacccggt
     1921 cctcgcgcgc cacaggtgtc tggtcacccg actcgcgcat cgcggccgaa gcctcgatgt
     1981 ccgcggactt ccccgctcgc tgggagacga tggcccggca gtcatggacc gaccaccatg
     2041 ccttcgtcgg tctggacggc gacccggtgc ggttcatcga ccaatacaat ctgcacggcg
     2101 gccccggcaa cgacggcccg gtcggtccag ccccgacgcc cgggcgcgtg ggcttcgccc
     2161 gcctcggcga gcggatcagc ggactggcgc catggtcccg accgttggga tcggacgagc
     2221 atcccttcac cgtcgaccgg gccacggtcc tgccgatcat gaccttcaat gtcgccgagt
     2281 acgcccggca gctgaccgag gccttcctga tggagggcgg gcggatcgtg cagcggacct
     2341 tcaaccatcc gtcggagctg gcggacctgg ccgagccggt ggtggtcaac tgcaccggct
     2401 atggcgcacg ggccctgttc ggggacgaga gcgtcgtgcc ggtgcggggc cagatcgcct
     2461 ggctgacccc gcagccggaa gtgacctatg ccctctacta ccggtccatc agcatggtcc
     2521 cgcggcacga cggcatcgtc atccaggcca atggcgcaag ccagatggtc ggctatggcg
     2581 tagcggacga gacgccggac cgggccgagg ccgaacaggc gctggcggcg ctggcgaccc
     2641 tgtttcagcc caggccgggc gcgaccgtgt aggtcgcctc ggtcttgctc gcgacctcgt
     2701 cgagggtgac gccctcggcc agctcgatca gctccagacc cgatccgtcc ggcttgacgt
     2761 cgaaaacgca caggtcggtg atgacacggc tgaccacgcc cgtaccggtg agggggaggg
     2821 tgcaggactt caggaccttg gactggccgt gcttgttggc gtgatccatg accacgacca
     2881 cgcgcttgac gccggcgacc agatccatgg cgccgcccat gcccttcacc agcttgcccg
     2941 ggatcatcca gttggcgatg tcgccgttct cggccacttc catggccccg aggatggaca
     3001 ggttgatgtg gcccccgcgg atcatggcga agctgtccga cgagctgaaa tagctgctct
     3061 cggggatctg ggtgatggtc tgtttgccgg cgttgatcag gtcggcgtcg acctcgtcct
     3121 cataggggaa ggggcccatg ccgagcatgc cgttctcgga ctggagggtg acctccatgc
     3181 cttccggaat gtagttggcg acgagggtcg ggatgccgat gccgaggttg acgtagaagc
     3241 cgtcctgcag ctctttcgcg gcgcgttcgg ccagttgttc gcgggtgcgg ggcattacga
     3301 cttccctcct tgggctaccg cttcgctact tgagcccggc tccctctgac gcacggtgcg
     3361 ctgttcgatg cgcttctcgg gcacggtctg gacgatgcgg tcgacgtaga tgccgggggt
     3421 gtggatggcg tcggggtcga gcgagccgac ggggacgatc tcctcgacct cgacgacgca
     3481 ggccttgccg gccgtggcca tcatcggatt gaagttgcgg gccgtcttgc ggaagacgag
     3541 gttgccgcgc tcgtcggcct tccacgcctt gacgatggac aggtcggcga cgaggccggt
     3601 ctccatgacg tagtcgcggc cgtcgaaggt gcggacctcc ttgccctcgg cgaccagggt
     3661 gccgacgccg gtggcggtga agaaggccgg gatgccggcg ccgccggcgc ggatgcgctc
     3721 ggcgagtgtg ccttgggggt tgaactcgag ctggagctcg ccggcgaggt actggcgctc
     3781 gaactccttg ttctcgccga cgtaggacga gatcatcttg gcgatctggc gggtctccag
     3841 cagctggccg aggccgaagc cgtcgacgcc ggcgttgttg gagatgaccg tcagcccctt
     3901 ggcgccgctg tcgcgcaggg cggcgatcat gtgctcggga atgccgcaca ggccgaagcc
     3961 gccggacatg acggtcatgc cgtcgaaggt caggccctcc agcgctgacg ccgcgtcggg
     4021 aaagatcttc cgcatcgctt cctcatattt tcaccgcctg atgaatatcg gcggtcttgg
     4081 ttcgtcccta acgtggggcg ggcggacggg caaggcgagc ggaaaaaaac acagtccgaa
     4141 gagtccgaag agtccactca gtccgcacgc aagaaagcca aacaccgtcc aatgcgtccg
     4201 tagagtccac tgcgtccact cccatccgcc cagaatagca gtatggcaca cggctgcgtc
     4261 agaatcggac gtgtccgacg gttacgggac gcgcgggctg cgcgccccgt gcgcggtagc
     4321 gggcgccctt gaggatgcca aggaaagcct cccagtgaat cccccaggtg accttccacg
     4381 tcaggagcgg atgggagacg aaggcgcgca ggagctggcc gtcggtcagg gggcggcggt
     4441 tgccggcgaa gctggcggtc aggacaggcg cgtcgccgcg acggaccgcg acgacgacgg
     4501 acacggcctc gcccggcggc cggacggtga agtcgtaggt caggtcctgg tccatgaagg
     4561 gggagacgaa gaactgcttc tcggctgtct ggcggacggg tgcggtcctt ccccctccac
     4621 catgcttcgc atggtcccgc tccccctgtg ggggaggaga gggaaccgcg accagatagc
     4681 tgtggcgttc gtggaaggtg ttcgtgactt cgtagagcag ggcggcgaga gcgccgtcgg
     4741 gcgtgtggca gaagaagacg ctgatcggat tgaagccgta accgaggatg cgcggcatgg
     4801 tcagcaggcg gatcgggccg ccggcgatgt cgatgccggc gttggccaga tggcgctcca
     4861 tctggagccg caagggacgg cctgaccggt cgccgtgatc ggcggttctg agggtgatca
     4921 ggccgaaggc gcggcggcgc agcaggcgca ggcggtcctg cagcccgtcc agttcgtcaa
     4981 ggtcgaccag caccatccag aggcggtagc gaaggcggtg gctgaaggtg cggacgcgct
     5041 ggtgcacgac ctcgccgacg tagagagcgc tgtggggtgc atctcccatc acctcgtcat
     5101 cctcgggctt gtcccgaggg cccatggcag tcccggagtc gtgccgcgga cgcagtgtgc
     5161 cgtatcagtg tgagggctcg tcacgcggaa agatgggccc tcgggacagg cccgatggtg
     5221 acgtggtact attcgtccgt t
//
LOCUS       DASVVN010000575         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_153375,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000575 DASVVN010000000
VERSION     DASVVN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153375"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1334
                     /locus_tag="VF686_09340"
     CDS             168..1334
                     /locus_tag="VF686_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HEX8662061.1"
                     /translation="MSLSDQVLAAVRAALPASEGGVPLHEPEFRGQEWAYLKDCLDTG
                     WVSSVGAYVDRIERDLEAITGAAHAVATSNGTAALDICLRLAGVKHGDEVLVPALTFV
                     ATANAVAYQGATPHFVDSEAATLGVDPVRLDAYLRETARFEGGACINTRTGATIRALM
                     VMHVFGHPCDLDGLAEVAARWKLVLVEDAAEGLGSRWNGRHVGNVGLVSALSFNGNKI
                     VTTGGGGAILTNDAELGRRAKHLTTTARVPHKWSFIHDEVGWNYRMPNLNAALGCAQL
                     ERLPDMMTRKAVLARRYAEAFRDVEGARLLSARPGAEVNHWLSALILDRPDMATRDAL
                     LETLNAAGFMARPLWTLMHRLPMYADCPRDALPVAEALEASVINLPSSAKLADA"
     gene            complement(1331..1951)
                     /locus_tag="VF686_09345"
     CDS             complement(1331..1951)
                     /locus_tag="VF686_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX8662062.1"
                     /translation="MRIGLCGTGGFAREVAPLAVIQAGADGVCHLVLETGEAALNGLP
                     VIPQDKATPETAISITIADAGQRRRIDAEMRAAGRTFVSIVAPTCVFRGPSQIGEGAI
                     LCDHVVITANVVIGRHFQANLFSYVAHDCVIGDFVTLAPRVCINGNTVIEDDVYVGTG
                     AVLKQGTPDKPLRIGRGAVIGMGAVVTKDVPAGAIVVGNPARALDR"
     gene            2009..>2845
                     /gene="neuC"
                     /locus_tag="VF686_09350"
     CDS             2009..>2845
                     /gene="neuC"
                     /locus_tag="VF686_09350"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX8662063.1"
                     /translation="METGLAKRRICVATSGRADYGLLTWPMRDIAAADGLSLQLLATG
                     THLEARHGLTVGQIEADGFAIDARVPMALTADDPASLARSSATGLSGAVEALQRLQPD
                     MLMLLGDRFETLALAQAAMLTGVPIAHVGGGDVTEGAIDEGIRHAITKLSHLHLVTHR
                     DAGRRVIRMGETPDRVHVVGNPGLDMLTRAPVMARDALETRLGRPLGARNLLVTFHPV
                     TTLPDKGLAELDALLKALEPLTDVTLWMTRANADPGGQAFSTALEAFAAGRSNVSLHD
                     ALG"
BASE COUNT          430 a          987 c         1004 g          424 t
ORIGIN      
        1 agcgaggtcg agcgtctgtg ggccgacagc ggtctgatgc agtccctgac cgggtgggag
       61 cccgagtacg gcggcggcga gggcttcggg cggggtctgc agcagaccat cgactggttc
      121 agcgatccgg tgaacctggc ccgctacaag gcagatcggt acgtgctgtg agcctgtccg
      181 atcaggtgct tgcggcggtc cgcgccgccc tgccggcgtc cgaaggcggc gttcccctgc
      241 acgagcccga gttccggggg caggagtggg cctatctgaa ggattgcctc gacacgggct
      301 gggtctcgtc cgtcggcgcc tatgtcgacc gtatcgagcg cgacctcgag gccatcaccg
      361 gcgccgccca tgcggtggcg acgtccaacg gcaccgccgc actcgacatc tgcctgcgcc
      421 ttgcgggggt gaagcacggg gatgaggtgc tggtcccggc gctgaccttc gtcgccacag
      481 ccaacgccgt cgcctatcag ggcgcgacgc cgcatttcgt ggactccgag gcggcgacgc
      541 tgggcgtcga tccggtgcgg ctggacgcat atctgcgcga gaccgcgcgc ttcgagggcg
      601 gcgcctgcat caacacccgc accggggcga ccatccgggc actgatggtc atgcacgtct
      661 tcggccatcc ctgtgatctg gacggactgg ccgaggtcgc ggcgcgctgg aagctggttc
      721 tggtcgagga cgcggccgag ggcctgggct cgcgctggaa cggccgccat gtcggcaacg
      781 tcgggctggt gtcggccctc agcttcaacg gcaacaagat cgtcaccacc ggcggcggcg
      841 gggccatcct gaccaacgat gccgaactgg gccggcgcgc caagcatctg accaccacgg
      901 cgcgggtccc gcacaaatgg tcgttcatcc atgacgaggt gggctggaac taccggatgc
      961 ccaatctgaa cgccgccctc ggctgcgccc agctggagcg gctgcccgac atgatgacgc
     1021 gcaaggcggt gctggcgcgg cgctatgccg aggcgttccg cgatgtcgaa ggcgcgcgcc
     1081 tgctgagcgc ccggcccggg gccgaggtca accactggct gagcgccctg attctcgacc
     1141 ggcccgacat ggcgacgcgc gacgccctgc tggagacgct gaacgcggcg ggcttcatgg
     1201 cccggccgct gtggacgctg atgcaccggc tgccgatgta tgccgactgt ccgcgcgatg
     1261 ccctgccggt cgccgaggcg ctggaggcca gcgtgatcaa cctgcccagc agcgcgaaac
     1321 tggccgacgc ctagcggtcg agcgcccgcg ccgggttgcc gacgacgatg gccccggccg
     1381 gcacgtcctt cgtcaccacc gcgcccatgc cgatgaccgc gccgcgcccg atgcgcaggg
     1441 gcttgtccgg cgtgccctgt ttcagcacgg cgccggtccc gacgtagacg tcgtcctcga
     1501 tcaccgtatt gccgttgatg cagacgcgcg gggcgagggt gacgaagtcc ccgatgacgc
     1561 aatcatgggc gacgtagctg aacagattgg cctggaaatg ccgcccgatc acgacattgg
     1621 cggtgatcac cacatggtcg cacaggatgg cgccctcgcc gatctgcgac ggcccccgga
     1681 agacgcaggt cggcgcgacg atggagacga aggtccgacc ggcggcgcgc atttccgcat
     1741 cgatccggcg tcgctggccg gcatcggcga tggtgatcga tatggcggtc tccggagtgg
     1801 ccttgtcctg ggggatgacg gggaggccgt tcagagccgc ctcgccagtc tcgagcacga
     1861 gatggcatac gccgtccgcg cccgcctgaa tcaccgccag gggggcgact tcgcgggcga
     1921 agccgccggt tccgcacaat ccgattcgca tgcgatcctc cccggacatg gtagcaggtt
     1981 gctaaggcgc gcggcctaca gcttgatcat ggagacgggg ttggcgaaac ggcggatctg
     2041 cgtggcgacc agcgggcggg ccgactacgg cctgctgacc tggcccatgc gcgacatcgc
     2101 cgccgccgac ggactgtcgc tgcagctgct ggcgacgggg acccatctgg aagcgcggca
     2161 cggcctgacg gtcggccaga tcgaggccga cggcttcgcg atcgacgccc gtgtgcccat
     2221 ggccctcacc gccgacgatc ccgccagcct cgcccgttcg agcgccaccg gcctgagtgg
     2281 cgcggtcgag gctttgcagc ggctgcaacc cgacatgctg atgctgctcg gcgaccggtt
     2341 cgagaccctg gccctggccc aggccgccat gctgacgggc gtgccgatcg cccatgttgg
     2401 cggcggcgac gtcaccgagg gcgcgatcga cgagggcatc cggcacgcca tcaccaaact
     2461 gtcccacctg catctggtca cccatcggga cgccggccgc cgggtcatcc gcatgggcga
     2521 gacgccggac agggtccatg tcgtcggcaa ccccgggctg gacatgctga cccgcgcgcc
     2581 ggtgatggcc cgcgacgcac tggagacgcg gctggggcgt ccgctcggcg cccgcaatct
     2641 gctggtcacc ttccaccccg tgacgacctt gcccgacaag gggctcgccg aactggacgc
     2701 cttgctgaag gcgctggagc cgttgaccga cgtcaccctc tggatgaccc gcgccaacgc
     2761 cgatccgggc gggcaggcgt tcagtacggc gctcgaagcc ttcgctgcgg gccgcagcaa
     2821 cgtcagcctg cacgatgcgc taggc
//
LOCUS       DASVVN010000576         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_443622,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000576 DASVVN010000000
VERSION     DASVVN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443622"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..581
                     /locus_tag="VF686_09355"
     CDS             222..581
                     /locus_tag="VF686_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8662064.1"
                     /translation="MARILLAEDDHSLRGFLARALERAGHSVVDCENGDDAIDALDQG
                     PWDLLLTDIVMPGADGIEVARVAAARQPGIRIMFITGFAAVALSAAQASPQAKVLSKP
                     VHLRDLVGEVERMVAAA"
     gene            662..736
                     /locus_tag="VF686_09360"
     tRNA            662..736
                     /locus_tag="VF686_09360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:694..696,aa:Val,seq:gac)
     gene            819..1058
                     /locus_tag="VF686_09365"
     CDS             819..1058
                     /locus_tag="VF686_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662065.1"
                     /translation="MALNLDTLLEGSPAYGVVDAKDGAVLFRRPGHEAAFDALAAEVL
                     DASGEEFEAIPLTDDGERCDKLFVAPLESRSFDPR"
     gene            1099..1527
                     /locus_tag="VF686_09370"
     CDS             1099..1527
                     /locus_tag="VF686_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662066.1"
                     /translation="MTSPAKFRRLLDLPGVADLELKALMKPRHADPDARAEFPDIDAT
                     AQAAFGLTVEAAENVVLPADWDGIEHLEGFDLTDAFAAEGWDVTDDRRKPLRMLVHFA
                     LPLALAMRGVAGELPFQPEDTTPEPWGAGMAAEARRFRKR"
     gene            1664..2443
                     /locus_tag="VF686_09375"
     CDS             1664..2443
                     /locus_tag="VF686_09375"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX8662067.1"
                     /translation="MNGAIYAAGAIGLGLTGLAFGDFALQWQPVPKGVPAREALSVIS
                     ALALLAAGLALLWRRSALYGALALAIMYGLWSFVLKTPALIARPLEMASWLGPAETLA
                     MAAGGLTLAALTLPTGVTEARMRLTARIAFGLCQPVFGLSHFAYAGFTAAMVPAWIPG
                     GGMFWAWATGVAHVAGGLAILSGVQARLAATLLTLMYAGFALLLHAPRVAAAPAAHIE
                     WVMLGIAASICGSAWAIRAGLEPAARPWGFLDRRDPAAATP"
     gene            complement(2450..3151)
                     /locus_tag="VF686_09380"
     CDS             complement(2450..3151)
                     /locus_tag="VF686_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8662068.1"
                     /translation="MTDLSAFPITGRWPAQHPDRLQLYSLNTPNGVKVSIMLEEIGLP
                     YEPHLVDISQNETWGPEYLSLNPNGKIPAIIDPDGPGGKPLALFESGAILIYLAEKTG
                     KLLSADPATRYQTIQWVMFQMGAIGPMFGQLGFFHKFAGREYEDKRPRDRYVAESKRL
                     LGVLETRLQGRDWIMDDYSIADVATLGWVRNLIGFYGARELVGFDELKNVPAWLERGL
                     ARPAVQRGLMIPAKP"
     gene            3277..4677
                     /locus_tag="VF686_09385"
     CDS             3277..4677
                     /locus_tag="VF686_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8662069.1"
                     /translation="MSDLFPIPSPADWRALAEKALKDRPLESLVHLDADGLAVRPLYA
                     GATGVTALSAPRPSDADGRAWDVRTLIEGDDATEVNAYALSDLEGGAASILLKGAILS
                     DSEPLSRALDGVALELAAVGLDAGLDGPEAANALAVAAKGSPRAKLMFHMDPLSAFAE
                     TGVAPRSIEEHLTLAANTAARHAGAYPEASFFLATGRVAHEAGGSIAQELGFAAANAV
                     ALIRAGMAAGLSAEQALKGTVLGVSVDQEYFDGLAKVRALRLIWRSIGKAFGAEAPAM
                     IEARSSRRMLSARDPWPNLLRLTAAGFAGAVGGADAVLLDGLSRANGRPDAFARRQAR
                     NTQAVLMEEANLGRVDDPAAGSWFLDARTRDLAEAGWAEFQRIEGEGGLIVALRTGDI
                     QDRVAAARAEREAALAGGTAHLVGVTKFVDPDPRPALFEPEPEPAVGSGGDACEPLPP
                     IRFAEPFETQSWEAAR"
     gene            4674..>5218
                     /locus_tag="VF686_09390"
     CDS             4674..>5218
                     /locus_tag="VF686_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8662070.1"
                     /translation="MSAPDFTRIPLDLGTTGTGPARAAWNTPEGLTVETAYGPEAIDG
                     LAFIHGLPGIAPYVRGPYPTMYAGNPWTVRQYAGFSTAEASNAFYRRNLAAGQKGLSI
                     AFDLATHRGYDSDHPRVKGDVGMAGVAIDSIYDMRTLFDGIPLDQMSVSMTMNGAVLP
                     ILALYVVAAEEQGVPHAALTGT"
BASE COUNT          765 a         1892 c         1731 g          830 t
ORIGIN      
        1 gccggaatgg ggcgaggcga agacgaaccg gccccccgct gcgccttcca ccacggtgaa
       61 gcccgcctga tcctcgccgc cgtcccctcg attcatgccg cagccatagc ctgttttcgc
      121 cactcatcca caaaggaccg ccttggcatt ccgctgattt tcatggcggg tttaccagtg
      181 caggcctagc ctcagggaaa gaagaccggg gcgaaaatca tatggcgcga atccttctgg
      241 ccgaagacga ccactccctg cgcgggttcc tggcccgcgc cctggagcgc gccggccatt
      301 cggtggtcga ttgcgagaac ggcgacgacg ccatcgacgc cctcgatcag ggcccgtggg
      361 acctgctgct gaccgacatt gtcatgcccg gcgccgacgg catcgaggtg gcgcgcgtcg
      421 ccgccgcccg ccagccgggc atccgcatca tgttcatcac cggctttgcc gccgtggccc
      481 tcagcgccgc ccaggccagc ccgcaggcga aggtcctgtc caagccggtc caccttcgcg
      541 acctcgtcgg cgaagtcgaa cgcatggtgg cggcggcctg acgtagcctc ttgcggattg
      601 ttagccctgc gggttataga ccgcgccttc ccgttccgga cccgtccgga accgccacgg
      661 cggacgcgta gctcagcggg agagcacttc gttgacatcg aaggggtcac aggttcaatc
      721 cctgtcgcgt ccaccattct ctccctgaca atccagcctg tccgcgcccg cgacgatatc
      781 ggagggaaac ctttggccgg ttcggggatt ggtccccaat ggccctcaat ctcgacaccc
      841 ttctcgaagg ctctccggca tacggcgtcg tcgacgccaa ggacggagcc gtgctgttcc
      901 gaagacccgg acacgaggcg gcgttcgacg ccctcgccgc cgaggtgctg gacgcttcgg
      961 gggaagagtt cgaagccatt ccgctgaccg atgacggcga gcgctgcgac aagctgttcg
     1021 tcgcgccgct ggaaagccgg tccttcgacc cgcgctaggc gtcgttgaca tcgaccctgc
     1081 cgcgggctca atccgcgcat gaccagcccc gccaagtttc gccgcctgct cgatctgccc
     1141 ggtgtcgccg atctggaact gaaggcgctg atgaagccgc gccacgccga tcccgacgca
     1201 cgggctgaat tccccgacat cgacgcgacc gcccaggccg ccttcggcct gaccgtcgag
     1261 gccgcggaaa acgtcgtcct gcccgccgac tgggatggga tcgagcatct ggagggcttc
     1321 gaccttaccg acgccttcgc ggccgagggc tgggacgtaa cggacgaccg gcgcaaaccc
     1381 ctgcgcatgc tggtccattt cgccctgccg ctggcgctgg cgatgcgcgg cgtcgccggg
     1441 gaactgccgt tccagcccga ggacacgacg cccgagccct ggggcgccgg catggccgca
     1501 gaggccagac gcttccgcaa acggtagatc acgcggcgca agacgccttc atcggcattc
     1561 gtcggcaaac ctcgggcggc ggctgttgcc gcctgcgcga aaaagggccc tgctgtcccg
     1621 tcggcggccg ggccgccgat attgcacggg ggcggcgatg cggttgaacg gggcgatcta
     1681 tgcggccggc gcgatcggcc ttgggctgac gggcttggcg ttcggcgact tcgccctgca
     1741 atggcagccc gtgccgaagg gtgttccggc ccgcgaggct ctttctgtga tcagcgccct
     1801 cgcgctgttg gcggcgggct tggccctgct ctggcggcgt tcggcgctgt acggcgccct
     1861 ggcccttgcg atcatgtacg gcctctggtc gttcgtcctg aagacgcccg cgctgatcgc
     1921 cagacccttg gaaatggctt cctggctcgg acccgccgaa accctggcga tggcggcggg
     1981 cggtctgacg ctggccgccc tgaccttgcc aaccggagtc accgaggccc ggatgcggct
     2041 gaccgcacgg atcgccttcg gcctgtgcca gcccgtcttc ggcctcagcc atttcgccta
     2101 tgcgggtttt accgccgcta tggtgccggc gtggattccc gggggcggga tgttctgggc
     2161 ctgggcgacc ggggtcgccc acgtcgccgg aggtctggcg atcctttccg gcgttcaggc
     2221 gcggctggcg gcgaccctgc tcacactcat gtacgccggc ttcgccctgc ttctacatgc
     2281 gccaagggtg gcggccgcgc cggccgccca tatcgaatgg gtcatgctgg gcatcgccgc
     2341 ctcgatctgc ggctcggcct gggcgatccg cgccgggctg gagccggcgg cgcggccgtg
     2401 gggcttcctc gaccgcaggg acccggccgc ggcaaccccg taaccgccgt cagggtttgg
     2461 ccgggatcat cagcccgcgc tgcacggcgg ggcgggccag accgcgctcc agccaggccg
     2521 gaacgttttt cagctcatcg aacccgacca gttcgcgcgc gccatagaag ccaatcaggt
     2581 tccgcaccca tcccagcgtc gcgacgtcgg cgatcgaata gtcgtccatg atccagtcgc
     2641 gcccctgaag ccgcgtctcg agcacaccca gaagccgctt cgactccgcg acataacggt
     2701 cgcgcggacg cttgtcctca tactcccgcc ccgcgaattt gtggaagaag ccgagttgtc
     2761 cgaacatcgg gccgatggcg cccatctgga acatgaccca ctggatcgtc tggtagcggg
     2821 tggccggatc ggccgacagc agcttgccgg tcttctcggc gaggtagatc aggatcgccc
     2881 ccgattcgaa cagcgccagc ggcttcccgc cgggaccgtc gggatcgatg atggccggga
     2941 tcttgccgtt cgggttcagc gacagatatt cggggcccca cgtctcgttc tgcgagatat
     3001 cgacgaggtg cggttcgtac ggcagcccga tctcctccag catgatcgag accttcaccc
     3061 cattgggcgt gttcagcgaa tacagctgca gccggtcggg atgctgcgcc ggccagcggc
     3121 cggtgatcgg aaaggcggac aggtcggtca tggggagact ccttgagggg acgccaccca
     3181 tgttggaagc cgcaagctga accgcaacac cctgagtggc ggaacggacg ccgcccgggt
     3241 ctggaccggg gcgcgcccgc gttgcattgg aggcggatgt ccgatctgtt ccccatcccc
     3301 tctcccgcag actggcgcgc gctggccgaa aaggcgctga aggaccgacc gctggaaagt
     3361 ctggtgcatc tggacgccga cggtctggcc gtgcgacccc tgtatgcggg cgcgaccggc
     3421 gtgacggccc tgtcggcgcc ccgcccgtcg gacgcagacg gccgggcttg ggacgtgcgc
     3481 accctgatcg aaggcgacga tgcgaccgag gtcaacgcat acgccctgtc cgacctcgaa
     3541 ggcggcgcgg cctccatcct gctgaagggc gcgatcctgt cggattccga gcccctgtcg
     3601 cgcgcgctgg acggcgtggc gctggaactg gcggcggtcg gtctcgacgc cggcctcgat
     3661 ggccccgagg cggccaatgc gttggccgtc gcggccaagg gttcgccccg cgcgaagttg
     3721 atgttccaca tggacccgct gtccgccttc gccgaaacgg gcgtcgcacc gcgttcgatc
     3781 gaggagcatc tgacgctggc ggccaacacc gccgcgcgcc atgcgggcgc ctatcccgag
     3841 gcgtccttct tcctcgcgac cggccgggtc gcgcatgagg ccggcggctc catcgcccag
     3901 gaactgggct tcgccgccgc caacgccgtg gccctgatcc gcgcgggcat ggcggccggc
     3961 ctgtcggcgg aacaggcgct gaagggcacg gtcctcggcg tctctgtcga ccaggaatat
     4021 ttcgacggcc tcgccaaggt ccgcgccctg cgcctgatct ggcgctccat cggcaaggcc
     4081 ttcggcgcag aagcgcccgc gatgatcgag gcgcgctcgt cgcggcggat gctgtccgcg
     4141 cgcgatccgt ggcccaatct gctgcggctg acggctgcgg gcttcgccgg cgcggtcggc
     4201 ggtgcggacg ccgtgttgct ggacggcctg tcgcgcgcca acggccgccc cgacgccttc
     4261 gcccggcgtc aggcccgcaa cacccaggcg gtgctgatgg aggaagccaa cctcggccgc
     4321 gtcgacgacc cggccgccgg ctcatggttc cttgacgccc gcacccgcga cttggccgag
     4381 gccggctggg ccgagttcca gcgcatcgag ggcgaggggg gcctgatcgt cgccctgcgc
     4441 accggcgaca tccaggatcg cgtcgccgcc gcccgcgcgg aacgcgaggc ggcccttgcc
     4501 ggcggaacgg cgcatctcgt cggcgtcacg aagttcgtcg atcccgatcc ccgtcccgcc
     4561 ctcttcgaac ccgagcccga accggccgtc ggaagcggcg gcgacgcctg cgagcccctg
     4621 ccgcccatac ggttcgccga accgttcgaa acccagagct gggaggccgc ccgatgagcg
     4681 cccccgactt caccagaatc ccgctcgacc tcgggacgac cggaaccggc cccgcccgcg
     4741 ccgcctggaa cacgcccgag ggcctgaccg tcgagaccgc ctacggcccc gaggccatcg
     4801 acggcctcgc cttcatccac ggcctgcccg gcatcgcccc ctatgtccgg ggcccctatc
     4861 cgaccatgta cgccggcaac ccgtggaccg tccgccagta cgccggcttc tcgaccgccg
     4921 aggcgtccaa cgccttctac cgccgtaacc tcgccgccgg tcagaagggg ctgtcgatcg
     4981 ccttcgacct ggccacccac cgcggctacg acagcgacca tccgcgcgtg aagggcgacg
     5041 tcggcatggc cggcgtggcg atcgacagca tctacgacat gcggaccctg ttcgacggca
     5101 ttccgctgga ccagatgtcg gtgtccatga ccatgaacgg cgccgtcctg ccgatcctgg
     5161 ccctctatgt cgtcgccgcc gaggaacagg gcgttcccca cgcggccctg accgggac
//
LOCUS       DASVVN010000577         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_181599,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000577 DASVVN010000000
VERSION     DASVVN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181599"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VF686_09395"
     CDS             complement(<1..558)
                     /locus_tag="VF686_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8662071.1"
                     /translation="MPPVLSLQSVSKTYGARRALDGVSLEVGPGEMVGLIGPSGSGKS
                     TLLRAVTGLVAVDPGAGRIEAFGEPVQAGGRISGAIRRTRCRLGFIFQQFNLVGRLSL
                     FSNVMVGALGRLPGWRGLLGLWPAADRQRAMAALHRVGIADYAAQRANTLSGGQQQRA
                     AIARALVQGANAILADEPVASLDPVS"
     gene            819..2084
                     /locus_tag="VF686_09400"
     CDS             819..2084
                     /locus_tag="VF686_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX8662072.1"
                     /translation="MRTYHRPLDLVRERSPERPVALVRQDCVAVAGRWFQENFKGDVL
                     YAVKANPSPWVIKTLWENGVTGFDVASIPEIELVAEHAPGARMAFMHPVKSRAAIRRA
                     YFDFGVKTFALDCVEELDKILEATDGAGDLDLIVRMAVQADGAQYSLSGKFGVSQEQA
                     PALLLAARRATTGLMGVSFHVGSQCMRPTAFQAAMAQCSRAIARAGVFVDVVDVGGGF
                     PSVYPGMIPPDMADYVDSIHRGFDEMKVSETTQLWCEPGRALVAEASSILCKVELKKG
                     DALYVNDGSYGSLFDATHTKWPFPVKLWRAEDESVAELRPFKLYGPTCDSADVMNGPF
                     WLPADVREGDYIEIGMLGAYGVAMTTRFNGYGDTETVAVEDAPMASMFGLAKRTIRLP
                     RQRDQEEERKVVRLSRPKGAAGKKRRRRY"
BASE COUNT          346 a          764 c          763 g          326 t
ORIGIN      
        1 ggagacggga tccagcgagg cgaccggctc gtcggcgagg atggcgttgg cgccctgcac
       61 cagcgcgcgg gcgatggcgg cgcgctgctg ctgcccgccc gacagggtgt tggcccgctg
      121 cgccgcgtag tcggcgatgc cgacccggtg cagcgccgcc atggcccgct gccggtccgc
      181 cgccggccac aggccgagca ggccgcgcca gccgggcagg cgaccgaggg cgccgaccat
      241 gacgttggag aacagcgaca gccgcccgac caggttgaac tgctggaaga tgaagccgag
      301 gcggcagcgg gtgcgccgta tggcgccgct gatccggccg ccggcctgca ccggctcgcc
      361 gaaggcttcg atgcggcctg cccccgggtc gacggcgacc aggccggtca ccgcgcgcag
      421 cagcgtcgac ttgcccgatc ccgacgggcc gatgaggccg accatctcgc cgggcccgac
      481 ctccaggctg acgccgtcga gcgcgcggcg cgcgccgtag gtcttcgaca ccgactgcag
      541 gctcaggacg ggcggcatga actcactggg ctgaggatcc ggaattgatc gccgcgcgag
      601 gcgacagttt cgtgacggcg ccccttcggc cggccggtta accgcgcctt taccatatgc
      661 agcgcttctc acctgtgaag accgcgacca aatgcgcggg cttcaggcct ttacaggggg
      721 ggcgacctcc cttatttgcg cgcttcccgg cggaccccgg aacagtcgaa acgctgctaa
      781 cgccggtctg ggtcaacaat ttcagggggt tacgtgaatt gcgcacgtac cataggcccc
      841 tggacctggt ccgtgagcgg tcccccgagc gtcccgtcgc cctcgtgcga caggattgcg
      901 tggccgtagc aggacgctgg ttccaggaaa acttcaaagg cgacgttctc tacgccgtta
      961 aggccaaccc ctctccctgg gtcatcaaga ccctgtggga gaacggggtc acgggcttcg
     1021 acgtggcgtc gattcccgag atcgagctgg tcgcggaaca cgcgccgggc gcccgcatgg
     1081 ctttcatgca cccggtgaag agccgcgccg cgatccgtcg cgcctacttc gacttcgggg
     1141 tgaagacctt cgccctcgac tgcgtcgagg agctggacaa gatcctcgag gcgaccgacg
     1201 gcgccggtga cctcgacctg atcgtccgca tggccgtgca ggccgacggc gcgcagtact
     1261 cgctgtccgg caagttcggc gtcagccagg agcaggcccc ggcgctgctg ctggccgcgc
     1321 gccgcgccac caccggtctg atgggcgtct cgttccacgt gggcagccag tgcatgcggc
     1381 cgaccgcctt ccaggcggcc atggcccagt gctcgcgcgc catcgcgcgg gccggcgtgt
     1441 tcgtcgacgt ggtcgacgtg ggcggcggct tcccgtcggt ctacccgggc atgatcccgc
     1501 ccgacatggc cgactacgtg gactccatcc accgcggctt cgacgagatg aaggtctcgg
     1561 agacgacgca gctgtggtgc gaacccggcc gcgcgctggt ggcggaagcc tcgtcgatcc
     1621 tgtgcaaggt cgagctgaag aagggcgacg cgctctacgt caacgacggc agctacggca
     1681 gcctgttcga cgccacgcac accaagtggc ccttcccggt gaagctgtgg cgcgcggaag
     1741 acgagtcggt cgccgagctc aggccgttca aactgtacgg cccgacctgc gactcggccg
     1801 acgtgatgaa cggcccgttc tggctgccgg ccgacgtgcg cgaaggcgac tacatcgaga
     1861 tcggcatgct gggcgcctac ggcgtggcca tgacgacgcg cttcaacggc tacggcgaca
     1921 ccgaaacggt cgcagttgaa gacgcgccga tggcctccat gttcggcctc gccaagcgca
     1981 ccatccgcct gccgcgccag cgcgaccagg aggaggagcg caaggtcgtc aggctttcgc
     2041 gtcccaaggg cgccgcgggc aagaagcggc gccgccggta ctgagtttct ccgcccgccc
     2101 ctcggggcgg gcggagatca agatccctcg gcccgtcgct ccgtccgggc cgctggtgag
     2161 atctggacgg gcgctcaaac catgagggaa accttctga
//
LOCUS       DASVVN010000578         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_468669,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000578 DASVVN010000000
VERSION     DASVVN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468669"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF686_09405"
     CDS             <1..493
                     /locus_tag="VF686_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8662073.1"
                     /translation="AAAAAAHAAGRKVAITLSDTFVVARWRAELLAFIEASADIVLAN
                     EGELAALFETEDFDAAAARLASMVEVAAITRGEAGSVVVAGDERVAVAAFPVARVVDT
                     TGAGDQYAAGFLLGLARGLSLEDAGKLGSLAASEVIAHWGPRPMTSLEALAKDAGLVL
                     NPA"
     gene            complement(480..1022)
                     /locus_tag="VF686_09410"
     CDS             complement(480..1022)
                     /locus_tag="VF686_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX8662074.1"
                     /translation="MSRRDDLSPDDRRIWARVAGSVTPAKPKKAPRIVPGAAIVDPPA
                     APHPKSPPKPRGVPDPWTVAPTPSRPRGLPEELEPRRQRRLSRERDPIEARIDLHGFG
                     RFQAQDALTAFLLGAQARGYRSVLVITGQGRRGGTGVIRASVHEWLQGPALRGVVSGF
                     ASAARHHGGDGALYVTIRRG"
     gene            complement(1019..1279)
                     /locus_tag="VF686_09415"
     CDS             complement(1019..1279)
                     /locus_tag="VF686_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8662075.1"
                     /translation="MAIRVQLDRILVERRMSLTELADRVGVTLANLSILKTGKARAIR
                     FTTLDALCRELNCQPGDLLVQTPGPQDPGSDEDADGNAPGTT"
     gene            complement(1303..1809)
                     /locus_tag="VF686_09420"
     CDS             complement(1303..1809)
                     /locus_tag="VF686_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX8662076.1"
                     /translation="MSSLLGVALGAAFWILALLTLAAALFFIFMAFVPMGPLGLTATS
                     EDGGTSVPVPRAYVLFGVGAITGYLGGFALILRHLRRIVYSLRIGDPFQPANVSRLKQ
                     IGLTLAVVTGGAWIGQTLVSRLVRGALEPPSMFDLVTPAFSVLVVFVLAEVFREGARL
                     RRESELTI"
BASE COUNT          341 a          755 c          868 g          360 t
ORIGIN      
        1 ggccgccgcc gccgccgccc acgcggccgg gcgcaaggtc gccatcaccc tgtccgacac
       61 cttcgtcgtg gcgcgctggc gcgccgaact gctggccttc atcgaggcct cggccgacat
      121 cgtcctggcc aatgagggcg agctggccgc cctgttcgag accgaagatt tcgacgccgc
      181 cgccgcccgc ctggcttcga tggtcgaggt cgccgccatc acccgcggcg aggccggttc
      241 ggtggtggtc gccggcgacg aacgcgtcgc cgtcgccgcc ttcccggtcg ccagggtggt
      301 cgacaccaca ggcgccggcg accagtacgc cgccggcttc ctgctgggtc tggcgcgcgg
      361 cctgtccctt gaagacgccg gcaagctggg ctcgctggcc gcttcggaag tcatcgccca
      421 ctggggcccc cgcccgatga cgtcgctgga agcgctggcg aaggacgccg ggctggtgct
      481 caaccccgcc tgatcgtcac ataaagcgcc ccgtcgccgc cgtgatggcg ggcggccgag
      541 gcgaagcccg agaccacgcc cctcagcgcc ggaccctgca gccattcgtg gacggaggcg
      601 cggatcacgc cggttccgcc ccgccggccc tgccccgtga tgaccagaac cgagcggtag
      661 ccgcgcgcct gcgcgcccag cagaaaggcg gtcagggcgt cctgggcctg gaagcggccg
      721 aagccgtgca ggtcgatcct cgcctcgatt ggatcgcgct cgcgcgacag ccggcgctgg
      781 cggcggggct ccagttcctc gggcaggccg cgcgggcggg acggagtcgg cgccaccgtc
      841 cacgggtctg ggacgccgcg cggtttcggg gggctcttcg gatgtggcgc ggcgggcgga
      901 tcgacgatgg cggcccccgg aacgatccgt ggggccttct tcggcttggc gggggtgacc
      961 gagccggcga cgcgggccca gatgcggcgg tcgtccgggc tcaggtcgtc gcggcggctc
     1021 atgtcgtgcc gggggcgttc ccgtcggcgt cctcgtcgga gcccggatcc tgcggccccg
     1081 gcgtctggac cagcaggtcg ccgggctggc agttcagctc gcggcacagg gcgtccaggg
     1141 tcgtgaaccg gatggcccgg gccttgcccg tcttcaggat cgacaggttg gcgagcgtca
     1201 cgccgacgcg gtcggccagc tcggtcagcg acatgcgccg ttcgacgagg atgcgatcta
     1261 gctggacgcg gatggccatg gcggggctct agcggacctt gttcagattg tcagttcgga
     1321 ttcgcggcgc aggcgcgcgc cctcgcggaa cacttcggcg aggacgaaga cgacgagaac
     1381 ggagaaggcg ggggtgacga ggtcgaacat gctgggcggc tccagcgcgc cgcgcaccag
     1441 ccgcgacacc agcgtctggc cgatccaggc gccgccggtg acgacggcca gggtcaggcc
     1501 gatctgcttc agccggctga cgttggcggg ctggaagggg tcgccgatgc gcaggctgta
     1561 cacgatgcgg cgcaggtgac gcaggatcag ggcgaagccg cccagatagc cggtgatggc
     1621 gccgacgccg aacaggacat aggcccgcgg gacgggcacg ctggtgccgc cgtcctcgga
     1681 cgtcgcggtc aggcccagcg ggcccatcgg cacaaaggcc atgaagatga agaagagggc
     1741 ggccgcgagg gtcagcagcg ccaggatcca gaaggcggcc ccgagggcga cgccgagaag
     1801 gctggacatg gatccgggac caattcccgg aagggcaggc gggcgaatcc cgagcggtat
     1861 cgtcttcggg aagcgcatgg tcgggcctcg tttcaggccg ggggtcgggg cgaggatgcc
     1921 gcgcggggtc cggcgaaccc acacgcgggc ttattcatcg cgattgtcat gccgatcgtc
     1981 aaagctcctg cgagacattc tgtttggcct accgcccttc gggctgcatg aggccgggga
     2041 agtcttacag ggcctggatc agggtggcga ccagggcggt cggcagggcg gcgagcagga
     2101 aggcgttcag gacgccgtgg ccaacctttt cgatgaagta ggaagcgttc atggtgttca
     2161 tggtctttct ccgtagttcc tgagtgaggg atgttttcac ccctcgttcg ttgttccgct
     2221 ggatcgatct tgccggtttt ggcttgttcg gggaacgtcg cggcgtccgt tcttgaagac
     2281 atagatagga cgtgcgttga agaaagtcat gttacatatc gttt
//
LOCUS       DASVVN010000579         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_127610,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000579 DASVVN010000000
VERSION     DASVVN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127610"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF686_09425"
     CDS             complement(<1..187)
                     /locus_tag="VF686_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8662077.1"
                     /translation="MTTIRSVAIVGAGQMGAGIAQAVAMGGYQVLLYDAAGDRVPQAQ
                     GAIAASLARQVARGLIDQ"
     gene            complement(314..619)
                     /locus_tag="VF686_09430"
     CDS             complement(314..619)
                     /locus_tag="VF686_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX8662078.1"
                     /translation="MGYAIVWLVDTILSLMTWAIILSAIFSWLFAFDVINRRNRFVAQ
                     VADFLDRITTPILAPFRRIIPPLGGIDISPIVVLLLINFLKILFMRLTAPILIQMLG"
     gene            772..847
                     /locus_tag="VF686_09435"
     tRNA            772..847
                     /locus_tag="VF686_09435"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:805..807,aa:Ala,seq:cgc)
     gene            866..1837
                     /locus_tag="VF686_09440"
     CDS             866..1837
                     /locus_tag="VF686_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX8662079.1"
                     /translation="MTQTASPAAARSTGPVTVAALYRFAAIAAREAVRDELDALCRPD
                     VRGTLLVAHEGLNGTIAGPAEAVERVLASIRALPGFAELDVKYATADVMPFHRLKVRI
                     KPEIVTMGQPDLDPLDAGTYVAPADWNALIADPDTVVIDTRNDYEAAVGAFAGAVQPN
                     TRSFRDFPDWFRTEGRALLDRPNPPKVAMYCTGGIRCEKATAFLKAEGVRDVFHLEGG
                     ILRYLETTPEAGSLWRGECFVFDERVAVGHGLSPGSHSLCRGCRMPVSEEGRTSPRYV
                     EGVACDRCADQRTEADRARYLERAKQVEMAERRGVAHIGAVQAPPED"
     gene            complement(1839..2111)
                     /locus_tag="VF686_09445"
     CDS             complement(1839..2111)
                     /locus_tag="VF686_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX8662080.1"
                     /translation="MTVTATANICNTRGLHARASAKFVKLASSFDAEVEVTRDGVTVN
                     ALSIMGLLMLGAGNGCGIEIRGEGPEAEAAVAALADLVERKFDEDQ"
     gene            complement(2108..2512)
                     /locus_tag="VF686_09450"
     CDS             complement(2108..2512)
                     /locus_tag="VF686_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8662081.1"
                     /translation="MIGLVIVTHGGLASEFLAAMEHVVGPQRGVAAICIGPEDDMERR
                     RRDIVDAANAVNDGDGVILLTDMFGGTPSNLAISVMEETHAEVIAGLNLPMLIKLASV
                     RGREPLESCVAHAQDAGRKYISVASWVLQGEK"
BASE COUNT          409 a          868 c          905 g          381 t
ORIGIN      
        1 tctggtcgat caggccgcgc gcgacctgac gcgccaggct ggcggcgatg gctccctgcg
       61 cctggggcac gcggtcgccg gcggcgtcgt acagcagtac ctgatagccg cccatggcca
      121 cggcctgggc gatgcctgcg cccatctggc ctgcgccgac gatggcgacg gatcggatcg
      181 tggtcattta gtccggttgg ctggcaggcg gggagcccgg tcggagcggc accttaggag
      241 actcgccgga cggcgcaagg ccggaagcgc gggaccgtga ggaaggggcg aaggcgtgtc
      301 acggcggtcg cgtttatccc agcatctgga tcaggatcgg cgcggtcagg cgcatgaaca
      361 ggatcttgag gaaattgatc agcagcagga cgacgatcgg cgagatgtcg atcccgccca
      421 gcggcgggat gatccggcgg aacggcgcca gaatcggcgt ggtgatccgg tccaggaagt
      481 cggccacctg ggcgacgaac cggttgcggc ggttgatcac gtcgaaggcg aacagccagc
      541 tgaagatggc cgaaaggatg atcgcccagg tcatgagcga gaggatggtg tcgaccagcc
      601 agacgatggc gtaacccata aagggactcc ggaaaccgat tgaacccccg cttctagcgt
      661 ggccgcgccc tacgtccaag gattccggcg ccgcagcggc gcgagggctc aagcccgttg
      721 acactccggc cccggccctc gtatgtcgcg ccctcgcctg cacccggaaa cggggccgta
      781 gctcagttgg tagagcgcgt cgttcgcaat gacgaggtca ggggttcgac tcccctcggc
      841 tccaccaggc acccgcacca ccgccatgac ccagacagcc agtccagccg cagcgcgctc
      901 caccgggccg gtgaccgtgg ccgccctgta ccggttcgcg gccatcgcgg cccgggaagc
      961 ggtgcgcgac gagttggacg cgctgtgccg cccggacgtg cgcggcaccc tgctggtcgc
     1021 ccatgaagga ctgaacggca ccatcgccgg cccggccgag gcggtcgagc gcgtcttggc
     1081 gagcattcgg gcgctgcccg ggttcgcaga gctggacgtc aaatacgcca cggcggacgt
     1141 catgccgttc catcgcctga aggtgcggat caagcccgag atcgtcacca tgggccagcc
     1201 ggacctcgac cccttggacg ccggaaccta tgtcgccccc gccgactgga acgccctgat
     1261 cgcggacccc gacacggtcg tgatcgacac ccgcaacgac tatgaggccg cggtcggggc
     1321 cttcgcgggg gcggttcagc ccaatacgcg cagctttcgc gattttcccg actggttccg
     1381 gaccgagggc cgcgccctgc tggaccggcc gaacccgccg aaggtagcca tgtactgcac
     1441 cggcgggatc cgctgcgaga aggcgaccgc cttcctgaag gccgaggggg tgcgggacgt
     1501 cttccatctg gagggcggca tcctgcgcta tctggagacg acgcccgagg ccgggagcct
     1561 gtggcgcgga gaatgcttcg tcttcgacga gcgggtcgcc gtcggccacg gcctgagccc
     1621 gggcagccac agcctgtgcc ggggctgccg gatgccggtc agcgaggaag gccggacatc
     1681 gccgcgctat gtcgagggcg tggcctgcga ccgctgcgcg gaccagcgca ccgaggcgga
     1741 ccgggcgcgc tatctggagc gggcgaaaca ggtggagatg gccgagcgcc ggggtgttgc
     1801 gcacatcggg gcggttcagg cgccgcccga ggactgacct actggtcctc gtcgaatttg
     1861 cgttcgacca gatcggcgag cgcggccacg gcggcctcgg cctccgggcc ttcgcccctg
     1921 atctcgatgc cgcagccgtt gccggcgccg agcatcagca ggcccatgat cgacagggcg
     1981 ttcaccgtca cgccgtcgcg ggtcacctcg acctccgcgt cgaagctgga ggccagcttg
     2041 acgaatttgg ccgaggcgcg ggcgtgcagg ccccgggtgt tgcagatgtt cgccgtggcc
     2101 gtgaccgtca tttctcgccc tgaagcaccc agctggccac cgagatgtat ttgcggcccg
     2161 cgtcctgggc gtgggccacg caggattcca gcggttcgcg gccgcgcacg ctggccagct
     2221 tgatcagcat cggcagattc agcccggcga tgacctcggc atgggtctcc tccatcaccg
     2281 aaatggccag gttggagggc gtgccgccga acatgtcggt cagcaggatg acgccgtcgc
     2341 cgtcgttcac agcgttggcg gcgtcgacga tgtcacgccg gcggcgctcc atgtcgtcct
     2401 cggggccgat gcagatggcc gcgaccccgc gctggggtcc cacgacgtgc tccatggccg
     2461 ccagaaattc cgaggccagt cccccgtggg tgacgatcac cagcccgatc atgacggcat
     2521 caccaagctc tcgcccccgg cgttcggcgc ccgtgagcgc gcc
//
LOCUS       DASVVN010000580         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_75504, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000580 DASVVN010000000
VERSION     DASVVN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75504"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF686_09455"
     CDS             <1..243
                     /locus_tag="VF686_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX8662082.1"
                     /translation="AEVQAARVLARPGMTRERFEAILSRQLPDTEKRRRADFVVDTGE
                     GLEAARARVGEIVGIVLAEGWSPPRRGLSKDAEARQ"
     gene            250..978
                     /gene="dnaQ"
                     /locus_tag="VF686_09460"
     CDS             250..978
                     /gene="dnaQ"
                     /locus_tag="VF686_09460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX8662083.1"
                     /translation="MSREIVLDTETTGFDPRTGDRMIEVGCIEIEDLLPTGRTFHRLI
                     HPERLIPPDAIRVHGITDEKVKDAPKFAEIVIDLCDFIGDAPIIAHNAQFDRSFIDHE
                     FGRCGRALFPEDRWIDTLKLAQTRFPGMANSLDALCKRYKISLVERSLHGALIDARLL
                     AEVYLELKGGKERRLDLSASRTVATAAAAAHAAAGYPPRPRPLEPRGSEVEHAAHEAF
                     LRATLKDLSLWKGYGLDMEKASAA"
     gene            complement(1038..1559)
                     /gene="secB"
                     /locus_tag="VF686_09465"
     CDS             complement(1038..1559)
                     /gene="secB"
                     /locus_tag="VF686_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX8662084.1"
                     /translation="MTDAAPPADANTAAPADTAQQGAPQGPGFRIVAQYVKDLSFENP
                     KAPDSLRVDGKPAIDMGVEMNAQGRPDGLFEVDLRLTIKATTDAMTVFNVELLYGGLF
                     ALQGVPEQDIEPLLLIECPRYLFPFAREIIARATSDGGFYPPFMLDPIDFAGIYVARQ
                     QSIARGPAEAGKA"
     gene            1750..2394
                     /gene="timA"
                     /locus_tag="VF686_09470"
     CDS             1750..2394
                     /gene="timA"
                     /locus_tag="VF686_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-related membrane protein TimA"
                     /protein_id="HEX8662085.1"
                     /translation="MPVPPVQIQIVIFAVIAAIVLFQLYNVLGKKVGRQPEDDARAKP
                     ATIPAGARPEQTAGRPNVLDAVTAASIAGLKARDPNFDPVRFLEGARQAHETIVRAYA
                     SGDRETLRPLLTPAVMASFEAGIAAREARGETEVAEFLHPARADLELASAEENRATAK
                     VRFLAELRNTVTSADASAEPQVEERRVAELWTFERTLGASDPNWVLARTEPAAA"
BASE COUNT          373 a          816 c          849 g          369 t
ORIGIN      
        1 gcggaggtgc aggcggcccg ggttctcgcc cggcccggca tgacccgcga gcggttcgag
       61 gccatcctgt cgcgccagct gcccgacacc gaaaagcgcc gccgggcaga cttcgtcgtc
      121 gacaccggcg aggggctgga ggcggcgcgg gcccgggtcg gggaaatcgt ggggatcgtt
      181 ctggccgagg ggtggtctcc gccccgtcgc ggcctttcga aagacgccga agcgcgccag
      241 taagagggca tgtcccgcga aattgttctc gacaccgaaa ccaccggctt cgaccctcga
      301 acgggcgacc ggatgatcga ggtcggctgt atcgagatcg aggacctgct gcccacgggc
      361 cgcacctttc accggctgat ccatcccgag cggctgatcc cgccggacgc gatccgggtg
      421 cacggcatca ccgacgagaa ggtgaaggac gcgccgaaat tcgccgagat cgtcatcgac
      481 ctgtgcgact tcatcggcga cgccccgatc atcgcccaca acgcccagtt cgaccgcagc
      541 ttcattgatc acgagttcgg ccgctgcggc cgggcgctgt tccccgagga ccgctggatc
      601 gacacgctga agctggccca gacccggttt ccgggcatgg ccaattcgct ggacgccctg
      661 tgcaagcgct acaagatcag cctggtcgag cgcagcctgc acggcgccct gatcgacgcc
      721 cgcctgctgg ccgaggtcta tctggagctg aagggcggca aggaacggcg gctagacctg
      781 tcggcctcgc gcacggtcgc gacggccgcg gcggcggccc atgcggcggc gggctatccg
      841 ccccgccccc gtccactgga accccgcggc agcgaggtcg aacatgcggc gcatgaggcc
      901 ttcctgcggg cgacgctgaa ggacctgtcg ctgtggaagg gctatggcct ggacatggaa
      961 aaggcgtccg cggcttaacg gacgcccttc tcacttcttg atctatcgct cgcctcgctg
     1021 tggcgagctg cttgagatca ggccttgccg gcttcggccg ggccgcgggc gatggactgc
     1081 tggcgggcga cgtaaatgcc ggcgaaatcg atcgggtcca gcatgaaggg cgggtagaaa
     1141 ccgccgtccg aggtggcgcg ggcgatgatc tcgcgggcga acgggaacag gtagcgcggg
     1201 cattcgatca gcagcagcgg ctcgatgtcc tgctcgggca cgccctgcag ggcgaacagg
     1261 ccgccgtaca gcagttcgac gttgaagacc gtcatggcgt cggtggtggc cttgatggtc
     1321 aggcgcaggt cgacttcgaa caatccgtcc gggcgcccct gagcgttcat ctcgacgccc
     1381 atgtcgatgg cgggcttgcc gtcgacgcgc aggctgtcag gggccttggg gttctcgaag
     1441 gacaggtcct tgacgtactg ggcgacgatg cggaagcccg ggccctgcgg cgcgccctgc
     1501 tgggcggtgt cggcgggcgc ggccgtgttg gcgtcggcgg gaggggcggc gtcggtcatc
     1561 ggtcgggtct gtctgaatag ggtgaacggc gtcgtttcag acgccccgga aggcgcggcg
     1621 actagcacga gggcgggggt gcggcaacga cgctcgcccg gcgcaggcag cggttcatgt
     1681 tgcgtggtcc tatatgagcc cctaatcgtc agtcgtcgcg gccgccgttt cgcggacgcc
     1741 agtcaaggaa tgcccgtgcc gccggtccag attcagatcg tcatcttcgc cgtgatcgcc
     1801 gctatcgtcc tgttccagct gtacaatgtg ctgggcaaga aggtcgggcg tcagcccgag
     1861 gacgacgccc gcgccaagcc ggcgaccatt cccgccggcg ccaggccgga gcagacggcc
     1921 ggccgtccga acgttctgga cgccgtcacc gccgcctcga tcgccgggct gaaggcccgg
     1981 gaccccaatt tcgaccccgt ccgcttcctg gagggcgctc gtcaggcgca tgagaccatc
     2041 gtgcgggcct atgcctccgg ggaccgcgag acgctgcggc cgctgctgac gccggccgtc
     2101 atggcctcgt tcgaggcggg catcgccgcc cgcgaggcgc gtggcgagac cgaggttgcc
     2161 gaattcctgc accctgcccg cgccgatctg gaactggcct cggccgagga aaaccgcgcc
     2221 accgccaagg tccgcttcct cgccgaactg cgcaatacgg tcacctcggc cgacgcctcc
     2281 gccgagccgc aggtcgagga acggcgcgtc gccgaactgt ggaccttcga acggaccctc
     2341 ggcgccagcg atccgaactg ggtcctggcc cgcaccgaac ccgccgccgc ctgatgcagg
     2401 gcggcgc
//
LOCUS       DASVVN010000581         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_416996,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000581 DASVVN010000000
VERSION     DASVVN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416996"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..883)
                     /locus_tag="VF686_09475"
     CDS             complement(77..883)
                     /locus_tag="VF686_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8662086.1"
                     /translation="MIRRSLLTLAAASLALAACGQGAAKMADADAPLIIGATAVPHAE
                     ILEHIKPLLAAEGVRVEIKVFNDYVQPNTQLMERRLDVNYFQTKPYLDEFNASRGADL
                     ITVAGVHVEPLGAYSRRFRSLDQLPNGAEVAVPNDASSIGRSLILLQKAGLIRLKDPA
                     NPLQSLRDIVDNPKNLRFRELESATLPRVLDQVDMAVINTNYALDAGLKPKTDALTLE
                     GADSPYVNYLVARPDNRTDPRIVALAAMLRSQAVKDFIAAKYDGAVIPAA"
     gene            complement(909..1574)
                     /locus_tag="VF686_09480"
     CDS             complement(909..1574)
                     /locus_tag="VF686_09480"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEX8662087.1"
                     /translation="MEALFANIDWPEIARATRDTLLMLAGSMALTVLFGVPLGVLLYL
                     SGKGRLAANPVLNAVLSLIVNILRSVPFIILLIVMLPVTVLLVGTSLGVAGAIPPLVV
                     GAAPFYARLVETALREVDKGVVEATQAMGGSTWQIVTRALLPEAMPGIIAGATVTAIA
                     LVSYTAMAGVVGAGGLGDLAIRFGYQRFQTDVMVVTVVLLLLLVQALQMLGDRLVASV
                     SHR"
BASE COUNT          275 a          539 c          547 g          235 t
ORIGIN      
        1 atccgcttga tctcgatcgg gcccagggcg gcgaaatgct cgcgcaccca ttcgccgaaa
       61 tcggcatcgt aggccatcag gcggccggaa tgaccgcgcc gtcgtacttg gcggcgatga
      121 agtccttgac cgcctggctg cgcagcatgg ccgcaagcgc cacgatccgc ggatcggtcc
      181 ggttgtcagg ccgcgcaacc agatagttca cataggggct gtcggcgccc tccagcgtca
      241 gggcgtccgt cttcggcttc agccccgcgt ccagcgcgta gttggtgttg atcaccgcca
      301 tgtcgacctg gtccagcacc cgcggcaggg tcgccgactc cagctcgcgg aagcgcaggt
      361 tcttcggatt gtcgacgatg tcgcgcaggc tctgcagcgg attggccggg tccttcaggc
      421 gtatcagacc ggccttctgc agcaggatca gcgagcgccc gatcgacgag gcgtcgttcg
      481 gcaccgccac ctccgccccg ttcggcagct ggtccagcga gcggaagcgg cgcgaatagg
      541 cccccagagg ctcgacgtgg acgcccgcca ccgtgatcag atcggccccg cgcgaggcgt
      601 tgaactcgtc cagatagggc ttggtctgga aatagttcac gtccagacgg cgctccatca
      661 gctgcgtatt gggctggacg tagtcgttga acaccttgat ctcgacccgg acgccctcgg
      721 ccgccagcag cggcttgata tgctccagta tctccgcgtg cggcacggcc gtggccccga
      781 tgatcagggg cgcgtcggcg tccgccattt tggcggcccc ctgcccgcag gcggcgagcg
      841 ccagcgaggc ggcggcgagg gtcaggagcg aacggcggat catgggcgag cctttcgggg
      901 gggagcgatc agcggtggga aacggaggcg accagacggt cgccgagcat ttgcagagcc
      961 tgcaccagaa ggagcagcag gacgacggtg accaccatga catcggtctg gaagcgctgg
     1021 tagccgaagc ggatggccag gtcgcccaga ccgccggcgc cgaccacgcc ggccatggcg
     1081 gtataggaca ccagggcgat ggcggtcaca gtggcgccgg cgatgatgcc gggcatggcc
     1141 tcgggcagca gggcgcgggt gacgatctgc caggtcgagc cgcccatggc ctgtgtggcc
     1201 tcgacgacgc ccttgtcgac ctcgcgcagc gccgtctcga cgaggcgcgc atagaagggc
     1261 gcggccccga cgaccagcgg cggaatggcg ccggctacac cgagcgacgt cccgaccagc
     1321 agcaccgtca ccggcagcat gacgatcagc aggatgatga agggcaccga ccgcaggatg
     1381 ttgacgatca gcgacagcac ggcgttcagc accgggttgg ccgccagccg ccccttgccc
     1441 gacaggtaca gcagcacgcc cagcggcacg ccgaacagca ccgtcagcgc catcgaccct
     1501 gcgagcatca acagggtgtc gcgggtcgcc cgcgcgattt ccggccagtc tatgttggcg
     1561 aacagtgctt ccatcacgcc gcccccacgc tttcga
//
LOCUS       DASVVN010000582         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_417042,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000582 DASVVN010000000
VERSION     DASVVN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417042"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VF686_09485"
     CDS             complement(<1..344)
                     /locus_tag="VF686_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662088.1"
                     /translation="MADANPVEGKAIEPRAGHESEGRPAALIAWGLYILSLPSANLLV
                     IVGLIVAYAGRSGATGLARAHIEAQIGLFWSVFWWTIGLWILIGVCVVSIAGAPVLGL
                     ALVPAAIVLGLGL"
     gene            complement(359..1063)
                     /locus_tag="VF686_09490"
     CDS             complement(359..1063)
                     /locus_tag="VF686_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662089.1"
                     /translation="MAEPGDPSNADDHDDLIGFATPASLEGRTRAPEAEAVDVPEPAP
                     KIEPEIEAAPPEPEPAPDLFTSRTVWTSAPVQPDPEPAMVAPAPIEPAPIPAPVEAAS
                     IFQHSPETPMQRRRRDPGADLPGGGIGLYAVYALILFAVPTLGVSAVIGLLAVTGRSG
                     PEDALSRSHFIYQQRTLWTAAVAAVAGLILLAAPFALGVPILFLLALWLVLRGASGVW
                     ALKSGHAIADPRGWWI"
     gene            complement(1131..1523)
                     /gene="folB"
                     /locus_tag="VF686_09495"
     CDS             complement(1131..1523)
                     /gene="folB"
                     /locus_tag="VF686_09495"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX8662090.1"
                     /translation="MNVTPLPFADADGPRLEGLTVFVRGLTLEAGIGIHDHELGRLQR
                     LVIDVTLELTPAPVERLGDTVNYETVAAAARAIVAEGHVGLVETFAQRLATACLADPR
                     VRRCAVRIEKPGALDGADAPGCEVVLSR"
     gene            complement(1527..2288)
                     /locus_tag="VF686_09500"
     CDS             complement(1527..2288)
                     /locus_tag="VF686_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8662091.1"
                     /translation="MTDRPARSGTALVTGGGKRIGRAIALELARAGFDVVIHYRSSED
                     AAMAVADEVRALGRKAATVKADLAVEAQVRTLVADAANAIDGPLAVLVNNASVFEDDR
                     VGSLGRETWDAHIETNLRAPIVLAETFAEQAPDGSAIVNLLDQRVLKPDPRFISYALS
                     RNGLWWATRTLAQALAPRIRVNGVGPGPTLPSVHQSAADFEAEARATPLQRAGSPQAV
                     ASAVRWLVDAELVTGQMIAVDGGQHLAWQTPDIVE"
     gene            2439..>2698
                     /locus_tag="VF686_09505"
     CDS             2439..>2698
                     /locus_tag="VF686_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8662092.1"
                     /translation="MTAWRTVIEPFARPVFFAYARATRGATLGVRGMAIHEDGRVLLV
                     KHTYLHGWWLPGGGVERGETCEQAILREMREEGGVIIEERPRL"
BASE COUNT          441 a          956 c          929 g          372 t
ORIGIN      
        1 aggcccaggc ccagcacgat ggcggcggga accagcgcca gtcccagcac cggcgcgccg
       61 gcgatgctga cgacgcagac gccgatcagg atccacagcc cgatcgtcca ccagaacacc
      121 gaccagaaca ggccgatctg cgcctcgata tgggcccggg ccaggcccgt ggcgccgctg
      181 cgtccggcat aggcgacgat caggccgacg atgaccagca ggttggcgct gggcagggac
      241 aggatgtaca ggccccaggc gatcagggcg gccggccggc cctcggactc atggccggcg
      301 cggggttcga tcgccttgcc ttcgacgggg ttcgcatcgg ccatctgtcg tctctccgtc
      361 agatccacca gccgcgcgga tcagcgatcg catggccgga cttcagggcc caaacgcctg
      421 acgcgccgcg aagcaccagc cataaggcca gaaggaacag gataggcacg cccagggcga
      481 acggcgccgc cagcaggatc agtcccgcca ccgccgccac ggccgcggtc cacagggtcc
      541 gctgctgata gatgaagtgc gagcgcgaca gcgcgtcctc gggtccggaa cggcccgtca
      601 cggccagcag gccgatcaca gccgacacgc ccagcgtcgg caccgcgaac aggatcaggg
      661 cgtagacggc gtacagaccg atccccccac cgggcagatc ggcgcccgga tcgcggcgac
      721 ggcgttgcat gggcgtctcc ggggagtgct ggaagatgga ggcggcctcg accggcgccg
      781 ggatgggcgc gggctcgatc ggggcgggcg cgaccatcgc gggttccggg tccggctgaa
      841 cgggggcgga cgtccagacc gtgcgcgacg tgaacagatc gggcgcaggt tccggctcgg
      901 gcggcgcggc ctcgatctcc ggctcgatct ttggcgcggg ttcaggcacg tcgacggctt
      961 ccgcctccgg cgcgcgcgtg cggccctcca gcgacgccgg cgtggcgaag ccgatcagat
     1021 cgtcgtgatc gtcagcattt gagggatcgc ccggttcggc catcaacggg tccttgtcat
     1081 cttgcccaag taatgacggc ccctcgcgcc ccgcgcaacc gcccagcaat tcaacgcgaa
     1141 agcacgacct cgcaccccgg cgcgtctgcg ccgtccagcg cgcccggctt ctcgatccgc
     1201 accgcgcaac gccgcacgcg cgggtccgcg agacaggccg tcgccagccg ctgggcgaag
     1261 gtctcgacca gacccacatg cccttcggcg acgatggcgc gcgccgctgc cgccaccgtc
     1321 tcatagttga ccgtgtcgcc cagccgctcg accggcgcgg gcgtcagctc cagcgtcaca
     1381 tcgatgacca gccgctgcag ccgtcccagc tcatggtcgt ggatgccgat cccggcctcg
     1441 agcgtcagcc cgcgcacgaa gaccgtcagt ccctccagac gggggccgtc ggcgtcggca
     1501 aagggcaggg gggtcacgtt cacggcctac tccacgatgt ccggggtttg ccaggcaaga
     1561 tgctggcccc cgtcgacggc gatcatctgg ccggtcacca gctccgcgtc caccagccag
     1621 cgcacggccg aggccacggc ttgcggactc ccggcgcgct gcaggggcgt ggcccgggcc
     1681 tcggcttcga aatcggccgc gctctgatgc accgagggca gggtcgggcc cggcccgacg
     1741 ccgttgacgc ggatgcgcgg cgccagcgcc tgcgccagcg tccgcgtcgc ccaccacagg
     1801 ccgttgcgcg acagggcgta ggagatgaag cgcggatcgg gcttcaacac cctctgatcc
     1861 agcagattga cgatcgcaga cccgtccggc gcctgttccg caaaggtctc ggccagcacg
     1921 atcggcgcgc gcagattggt ttcgatatgg gcgtcccagg tttcgcggcc cagcgagccg
     1981 acccggtcgt cctcgaacac ggaggcgttg ttgaccagca ccgccagcgg accgtcgatc
     2041 gcgttcgcag cgtcggcgac cagcgtccgc acctgcgcct cgacggccag atcggccttt
     2101 accgtcgcgg ccttgcgccc cagcgcgcgc acctcgtcgg ccacggccat ggccgcgtct
     2161 tccgacgagc gatagtggat gacgacgtcg aagccggccc gcgccagctc aagggcgatc
     2221 gcgcgcccga tccgcttgcc gccgccggtg accagcgcgg tgccagaccg ggcgggacga
     2281 tcagtcaacc cggccgaact cgatgaagtt gatggcggcc agaacggcga cgaagccgag
     2341 gatgatgtag agggccagca tggaaagctc cttgttgagg ccgcaataaa gcgccgcgcc
     2401 ctcctgttca agaacgcctg accgcttatc gttgcgccat gaccgcttgg cgcaccgtga
     2461 tcgaaccctt cgctcgaccc gtgttcttcg cctatgcccg ggcgacgcgc ggggccactc
     2521 tgggtgtgcg tggcatggcg atccatgaag acggccgcgt tctgctcgtc aaacacacct
     2581 atttgcatgg ctggtggttg ccgggcggcg gagttgagcg gggagagacc tgtgagcagg
     2641 cgatcttgcg cgaaatgcgt gaagagggcg gagtgatcat cgaggagcgc ccgaggct
//
LOCUS       DASVVN010000583         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_205211,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000583 DASVVN010000000
VERSION     DASVVN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205211"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VF686_09510"
     CDS             complement(<1..684)
                     /locus_tag="VF686_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8662093.1"
                     /translation="MVHRAGTGPAVIVMAEMPGISPHVARFARWVRDAGFTVYMPSLF
                     GMDGAVPTADEGAAVFRRACVSAEFRALGLNVSSPVTLWLRGLARVVHGECGGPGVGA
                     VGMCFTGNFALTMMLEPSMLAPVVCQPSLPLNDPAGLEISDEELGEIAERLERENLSV
                     LAYRFSGDAFCRAERFAAYSRALGPRFDGRVLPDPGPADTPRPPFFQQHVPAPHSVVT
                     VDLVDREGEP"
     gene            896..>1531
                     /locus_tag="VF686_09515"
     CDS             896..>1531
                     /locus_tag="VF686_09515"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8662094.1"
                     /translation="MASSNGAAWPLWLSGSIMGQWHRRGVLALGGAALAAGGVRTQVA
                     TPAALEAAIEQVRRMMTSANVPGAGIAVVHQGEVLQAQGLGLASLPFQVPAQATTLFH
                     LGSVSKQLTAALVLELVEAGAIALDDPVGRHARNLPASFATVPIRNLLSHTGGVPDYE
                     GLPGFETDRFIGREAFLSGAGALPTDFAPGEAWAYSNTGYVLLGYLLADVTG"
BASE COUNT          247 a          509 c          541 g          234 t
ORIGIN      
        1 cggctcgccc tcgcggtcga caaggtccac cgtcaccacg ctatggggcg cgggcacatg
       61 ctgctggaag aacggcggac gcggcgtgtc ggcgggcccg ggatcaggga gcacccgtcc
      121 gtcgaagcgc gggcccagcg ccctggaata ggccgcgaac cgctcggccc tgcagaaggc
      181 gtcgcctgaa aagcgatagg ccaggacgct gaggttctct ctctccaggc gttcggcgat
      241 ctcgcccagc tcctcgtcgg agatttcgag gccggccgga tcgttgagcg ggagagaggg
      301 ctggcagacg accggcgcca gcatcgacgg ctccagcatc atcgtcagcg cgaagttgcc
      361 ggtgaagcac atgcccacgg cgcccacgcc cggcccgccg cattcgccgt gcacaacacg
      421 ggcgaggcct cgcagccaca gtgtcaccgg gctggaaacg ttcagcccga gcgcgcggaa
      481 ctcggcgctg acgcaagcgc gacgaaagac ggccgcgcct tcatcggcgg tcgggacggc
      541 gccgtccata ccgaacaggg acggcatgta gacggtgaag ccggcgtcac gcacccatcg
      601 tgcaaaccgc gcaacatggg ggctgatccc gggcatttcg gccatgacga tgacggccgg
      661 accggttccg gcgcggtgga ccatcttggt ctcgcccagg atcgtcgtcg gtcgacgccc
      721 gaagtcggca aggtcatcgg gtcggagcag gttcgtcggc atgatctctc cagtcgggga
      781 gaagaaaatg cggcggcctg aacctgcggc ccagcggcga atgagacatt gatcgcggcg
      841 ggttcgccaa ccgcgtggtt cgcgcaaacg gcgggcttgc gccgcattct tcgacatggc
      901 ctcgtcaaac ggggccgcat ggccgctgtg gctttcgggg agcatcatgg ggcaatggca
      961 tcggcgcggc gtgctcgcgc tcggcggcgc ggcgctggcg gcgggtggtg tgcgaacgca
     1021 ggtcgcaacg cctgcggccc tcgaggcggc cattgagcaa gttcggcgaa tgatgacgag
     1081 cgcgaacgtc cccggcgccg ggatcgccgt cgtgcatcaa ggcgaggtcc tgcaggccca
     1141 aggcctgggc ctggcctcgc tgcctttcca ggtcccggcc caggcgacga cgctgttcca
     1201 tctcggatcc gtttccaaac agctgaccgc agccctggtc ctcgagctcg tcgaggcagg
     1261 cgcgatcgcc ctggacgatc cggtcggtcg ccatgcccgc aatctgccgg cgtctttcgc
     1321 caccgtcccg atcaggaacc tgttgagcca tacgggcggc gtgccggact atgagggcct
     1381 gcccgggttt gagaccgatc gcttcatcgg gcgcgaagcg tttctgtcgg gcgcaggcgc
     1441 gttgccgacg gacttcgcgc ccggggaagc ctgggcctac tcgaacaccg ggtatgtact
     1501 cctcggctac ctgctggcgg acgtcacggg g
//
LOCUS       DASVVN010000584         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_287536,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000584 DASVVN010000000
VERSION     DASVVN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287536"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="rplF"
                     /locus_tag="VF686_09520"
     CDS             <1..435
                     /gene="rplF"
                     /locus_tag="VF686_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX8662095.1"
                     /translation="SWTVADEIEVTQGDDGLNLAIREDTQRARAMWGLSRTLVDNMVV
                     GVTTGFEKSLDLVGVGYRAAMKGNALSLQLGFSHDVDIQPPAGVTFAVPKQTEIKITG
                     ADKQAVGQIAAVIRKLRPPEPYKGKGVRYTGEKVRRKEGKKK"
     gene            439..801
                     /gene="rplR"
                     /locus_tag="VF686_09525"
     CDS             439..801
                     /gene="rplR"
                     /locus_tag="VF686_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX8662096.1"
                     /translation="MALSLRQQAQRRTERTRRRLKAVANGRLRLSVHRSDKNISAQII
                     DDLNGVTIASASSLEGEKGKKAKGSDVAAAALIGKLVAERAIEKGIKDVVFDRGGYIY
                     HGRVKALAEAAREAGLNF"
     gene            814..1434
                     /gene="rpsE"
                     /locus_tag="VF686_09530"
     CDS             814..1434
                     /gene="rpsE"
                     /locus_tag="VF686_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX8662097.1"
                     /translation="MAQTPQRGGGGNDRNRRDNRNAPAADGPDSDIVEKLVHINRVAA
                     TVKGGRRFSFAALMVVGDGKGRVGFGHGKAREVPEAIRKATEEAKKTMIRVPLRENRT
                     LHHDGAGRWGAGKIMMRAAPPGTGVIAGGPMRAVLETLGVQDVVGKSTGSSNPYNMIR
                     ATFEALKVQSSPRQVASKRGKKVADLMGRRNDGASAPEAAPEAQEA"
     gene            1439..1636
                     /gene="rpmD"
                     /locus_tag="VF686_09535"
     CDS             1439..1636
                     /gene="rpmD"
                     /locus_tag="VF686_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX8662098.1"
                     /translation="MAKTESKTTVTVRQTGSPIRRKSDQRATLMGLGLNRMGRESTLE
                     DTPSVRGMIAKVAHLTEIVEK"
     gene            1810..2256
                     /gene="rplO"
                     /locus_tag="VF686_09540"
                     /pseudo
     CDS             1810..2256
                     /gene="rplO"
                     /locus_tag="VF686_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            2508..>3829
                     /gene="secY"
                     /locus_tag="VF686_09545"
     CDS             2508..>3829
                     /gene="secY"
                     /locus_tag="VF686_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX8662099.1"
                     /translation="MASAAEQLAANMNMGSFAKATELHKRLLFTLLALLVYRIGTYVP
                     IPGINSEAFLQFFQNPDGQRGILDMFNMFSGGAVERMAVFALNVMPYISASIIVQLMG
                     AVYPPWEKLRKEGGESGRKQLNQYTRYLTVVLALFQSAGIAIGLNAQPGLIDSPGLFF
                     IISTITTLTGGTMFLMWLGEQVTARGVGNGISLIIFAGIVAVLPGTVARMFGLAQQGQ
                     MSAFTLLFITAMMIAVVVFIVFMERAQRRLLIQYPKRQEGNRMAGGERSFLPLKVNTA
                     GVIPPIFASSLLMLPTTVAQMTANANLPSWMDWLPVVTAQMQHGQPVFMLLYGALIVF
                     FCFFYTSITFNPEDTAENLRKYGGFLPGIRPGKRTAEYLDYVLTRLTTIGAAYITAVC
                     LLPELMIGFYNVPFYLGGTSILIVVSVTMDTVTQIQSHLLAHQYEGLI"
BASE COUNT          709 a         1307 c         1198 g          615 t
ORIGIN      
        1 tcctggaccg tcgctgacga gatcgaggtc acccagggtg acgacggcct gaacctggcc
       61 atccgcgaag acacccagcg cgctcgcgcc atgtggggac tgtcgcgcac cttggtagac
      121 aacatggtcg tcggcgtcac cacgggcttc gaaaagagcc tggatctggt cggcgtgggc
      181 tatcgcgccg cgatgaaggg caacgccctg tcgctgcagc tcggcttctc gcacgacgtc
      241 gacatccagc cgcctgccgg cgtcaccttc gcggtgccca agcagacgga aatcaagatc
      301 accggcgccg acaagcaggc cgtcggtcag atcgccgccg tcatccgcaa gctgcgtccg
      361 ccggagccct acaagggcaa gggcgtgcgc tacacgggcg aaaaggtccg gcgcaaggaa
      421 ggcaagaaga agtaagtcat ggctctttct cttcgacaac aagcccagcg ccgtacggag
      481 cgcacccgcc gccgcctgaa ggctgtcgcc aacggtcgtc tgcgcctgtc ggtgcaccgt
      541 tcggacaaga acatctcggc ccagatcatc gacgacctga acggcgtcac gatcgcttcg
      601 gcctcctcgc tggaaggcga gaagggcaag aaggccaagg gttcggacgt cgcggccgcc
      661 gctctgattg gcaagctggt cgccgaacgc gccatcgaga agggcatcaa ggatgtcgtc
      721 ttcgatcgcg gcggctacat ctatcacgga cgggtgaagg cgctggcgga agccgcgcgt
      781 gaagccggcc tgaacttcta agggacgcga cagatggcgc aaacacccca acgcggcggc
      841 ggcggcaacg atcgcaaccg tcgtgacaac cgcaacgctc ccgccgccga tggtccggat
      901 tcggacatcg tcgagaagct ggtgcacatc aaccgcgtcg cagccaccgt caaaggcggc
      961 cgccggttct cgttcgcggc cctgatggtc gtcggcgacg gcaagggccg cgtcggcttc
     1021 ggtcacggca aggcgcgcga agtgccggaa gccatccgca aggcgacgga agaagccaag
     1081 aagaccatga tccgcgttcc gctccgcgag aatcgcaccc tgcaccacga cggcgccggc
     1141 cgttggggcg ccggcaagat catgatgcgc gccgcccctc ccgggaccgg cgtcatcgcg
     1201 ggcggtccga tgcgcgccgt gctcgaaacc ctcggcgtcc aggacgtcgt gggcaagtcg
     1261 accggttcgt cgaaccccta caacatgatc cgcgccacgt tcgaggccct caaggtccag
     1321 tcgtcgcccc gtcaggtcgc gtccaagcgc ggcaagaagg tcgccgatct gatgggccgc
     1381 cgcaacgacg gcgcctcggc tccggaagcg gcgcctgaag cccaggaagc ctgagaccat
     1441 ggccaagact gaatccaaga ccaccgtcac cgtccgtcag acgggctcgc ccatccgccg
     1501 caagtcggac cagcgcgcca ccctgatggg tctgggtctc aatcgcatgg gccgcgagtc
     1561 cacgctggaa gacaccccct cggttcgcgg gatgatcgcc aaggtcgccc acctgaccga
     1621 gatcgtcgaa aagtaagtcc cgctttcaag cgggagtggc gagtggttag tggcgagtga
     1681 cgagtgagga gagggccgga tcggcgtttt tccttgactc gccactcatc actcgccact
     1741 cgccactttc ttttttccaa gccgagtccg gatccgatcc gggcgctagc acccgaaagg
     1801 atcaggcaca tgaaactgaa cgaaatccgc gacaacgagg gcgcccacaa gaagcgcatg
     1861 cgcgtcggcc gtggcccggg ctcgggcaag ggcaagaccg ccggtcgcgg cgtcaagggc
     1921 cagaagtccc gttcgggcgt cgccatcggc ggcttcgaag gcggccagat gccgctgtac
     1981 atgcgtatgc ccaagcgcgg cttcaacaat cccaacgcgc tgaagctggc tgaagtgaac
     2041 ctgtggcgcc tgcaggacgc cgtggacgcc aagaagctgg acatcaaggg cgagatcaag
     2101 ggcgacgccc tggtcgcggc cggcgtgatc cgtcgcgtca aggacggcgt ccgcatcctc
     2161 ggcaccggcg agatcaagtc caagctgaac ctggtcgtct ggtcggcctc ggccggcgca
     2221 atcaaggcga tcgaagccgc cggcggcacc gttgttcagg agcgtatcgc tgcggaagcc
     2281 aaggccgccg cgcgcgtcga gaagcgcaac gccgccaagg gcaaggctcc ggctcccaag
     2341 gctccccgtg gcgacgccaa caagatctcg gcccgcacga cccggacggc cgccaaaaaa
     2401 taagcttgag gggcgctctc gctaagaggg cgcccttcgc ctatctggac cgtggcgccg
     2461 gattcctgtc cgatgcgccg cgttctgatc agtcggggtg acgacatatg gcttcggccg
     2521 ccgaacaact cgcagcaaac atgaacatgg gctcgttcgc caaggcgacc gagctgcaca
     2581 aacgtctgct gttcacgctc ctggccctgc tggtctaccg catcggcacc tatgtgccga
     2641 tcccggggat caactcggaa gccttcctgc agttcttcca gaaccctgac ggccagcgcg
     2701 gcatcctgga catgttcaac atgttctcgg gcggcgccgt ggagcgcatg gccgtcttcg
     2761 ccctgaacgt catgccgtac atctcggcgt cgatcatcgt ccagctgatg ggcgccgtgt
     2821 atccgccctg ggagaagctg cggaaagaag gcggcgagag cggccgcaag cagctgaacc
     2881 agtacacccg ctatctgacc gtggtcctgg ccctgttcca gtcggccggc atcgccatcg
     2941 gcctgaacgc ccagcccggc ctgatcgaca gccccggact gttcttcatc atttcgacca
     3001 tcaccacgct gaccggcggg accatgttcc tgatgtggct gggtgagcag gtcaccgcgc
     3061 gcggcgtcgg caacggcatc tcgctgatca tcttcgccgg catcgtcgcc gtcctgccgg
     3121 gcaccgtcgc ccgcatgttc ggtctggccc agcagggcca gatgtccgcc ttcaccctgc
     3181 tgttcatcac cgccatgatg atcgcggtcg tggtcttcat cgtcttcatg gagcgcgccc
     3241 agcgccgcct tctgatccag tatccgaagc gccaggaagg caaccgcatg gccgggggcg
     3301 agcgctcgtt cctgccgctg aaggtcaaca ccgccggcgt cattccgccg atcttcgcct
     3361 cgtcgctgct gatgctgccg accacggtgg cgcagatgac ggccaatgcg aacctgccgt
     3421 cgtggatgga ctggctgccg gtcgtcaccg cccagatgca gcacggccag cccgtcttca
     3481 tgctgctgta cggcgcgctg atcgtcttct tctgcttctt ctacacctcg atcaccttca
     3541 accccgagga cacggccgag aacctgcgca aatacggcgg cttcctgccc ggcatccggc
     3601 ccggcaagcg cacggcggag tatctggatt atgtcctgac gcgcctgacg acgatcggcg
     3661 cggcctacat cacggcggtc tgcctgctgc ccgagctgat gatcggcttc tacaacgtgc
     3721 cgttctatct gggcggcacc tcgatcctga tcgtggtcag cgtcaccatg gacaccgtca
     3781 cccagatcca gtcccacctc ctggcccacc agtacgaggg cctgatcaa
//
LOCUS       DASVVN010000585         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_100549,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000585 DASVVN010000000
VERSION     DASVVN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100549"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..76
                     /locus_tag="VF686_09550"
     CDS             <1..76
                     /locus_tag="VF686_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662100.1"
                     /translation="TRRYAKRQLTWFRNQTPDWRRVSP"
     gene            418..785
                     /gene="ssrA"
                     /locus_tag="VF686_09555"
     tmRNA           418..785
                     /gene="ssrA"
                     /locus_tag="VF686_09555"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            898..1386
                     /locus_tag="VF686_09560"
     CDS             898..1386
                     /locus_tag="VF686_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX8662101.1"
                     /translation="MADETPPVDEMHYEQLAQDALRGVVRSALERAALPEGIPGAHHF
                     YVTFKTRAPGVSVPPDIMAKYPDEMTVVLQHQYWDLKVEPTRFSVMLKFGGMPKVLSM
                     PYTAVTRFYDPSVQFLLQFEAPTTVEEIAAEVAAAEPVRTDPDGKDDGPKVVSLDQFR
                     KK"
     gene            1447..2229
                     /locus_tag="VF686_09565"
     CDS             1447..2229
                     /locus_tag="VF686_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEX8662102.1"
                     /translation="MGRVFVIVLAVWVALVSPACAQAARASAGRFEGWTSAIVAADWR
                     DGRGRPIQAFDNARRDLVKGFLNAGFPRATMVDYSLRPDVPSPATPQTVLDGLYDAAA
                     RGSAGCLLYFTSHGVPTAMVFGDAPRMTPDMMVNIVRRACGARPTVVIVSACYSGIFV
                     NALSAPNRMVLTAASRERTSFGCGADETYPWFDGCILETLPRAADFLALAAGARACVA
                     RKEQEHAVDLPSEPQLFVGSEMQLRLPTLRFNRPADGGRPGS"
     gene            complement(2192..2554)
                     /locus_tag="VF686_09570"
     CDS             complement(2192..2554)
                     /locus_tag="VF686_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEX8662103.1"
                     /translation="MTAGWIGIGKVRAREVGDAVEITIDGLTTQAKYYKPLVYEFMRK
                     EWSSRPSYGDYTVEILMEHVGDPPWMDLDNLAKALLDAIKGYLFHDDAQVARLLVERR
                     EGERERITIRVFPRPPAG"
     gene            2646..>2991
                     /locus_tag="VF686_09575"
     CDS             2646..>2991
                     /locus_tag="VF686_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX8662104.1"
                     /translation="MTNRTETDTFGPIEVASDRYWGAQAQRSLGNFKIGWEKQPLPIV
                     RALGIVKRAAAETNMALGKLDPAIGNTIVKAADEVIAGTLNDHFPLVVWQTGSGTQSN
                     MNANEVISNRAIE"
BASE COUNT          492 a         1045 c          947 g          507 t
ORIGIN      
        1 gacccggcgc tacgccaagc gccagctgac ctggttcagg aaccagacgc ccgactggcg
       61 acgcgtctcg ccctgatcga cacgacgtcg cgcctgcgtc gacattcccc agacttcgca
      121 tcatagtccc agcctgctgc cgccgtgcgg aggtccgcca tgattgccgt gttcgccctc
      181 gccctgctga tgaaccaggc gccgggagat ctgccccggt cgggagaccg cgtttcagcc
      241 gatcggcttc tctgccgtaa cgaacagcag cccgcgaccc ggattcccag gcgcgtctgc
      301 atgacccaat acgagcggag cggcgtcgag gagacggcgg atcagcaccg cgcccgcgtc
      361 cggcccgtga tcgacaatcg gcgtcccggg cgcggcatcg gccgctggta gcggccggcc
      421 cttcaattcc tcgccgccac agcctatatt ggtcctgtcc gggttcgccc ggactatggc
      481 gataaacgcg cttgtaataa gcggaccgga cccgggtgcg attcccggcg gctccaccat
      541 ttcttccccg cgttatcggc ggagcgagca tggggccgac tagcatcgac ggacgtgtaa
      601 agaagactgc tttcgcccgt cctggctcac cgtatcgggc cattttctaa ctgcgaacga
      661 taacttcgct ggagaagtcg ctctcgccgc ataagcggtt cgggtgattt cgaacactta
      721 agtcctgggg gatcagttcc tcaggcgggg ccgccagggg gcctgccaac agaacccctg
      781 gcactttccg gtcatcattg gttagctcgc cgcagaaacg cggcttttca ccacgtcgcc
      841 acctttcgcg agccgtccgg cgcagctatc agttcgcccg aactcctgag gcgacccatg
      901 gccgacgaaa ctcccccagt cgacgagatg cactacgagc agctggcgca ggacgccctg
      961 cgcggtgtcg tgcgctcggc gctggagcgg gcggccctgc ccgaaggcat tcccggggcg
     1021 caccacttct acgtcacctt caagacgcgc gcgcctggcg tcagcgtgcc gcccgacatc
     1081 atggccaaat atccggacga gatgacggtc gtgctgcagc accagtactg ggacctgaag
     1141 gtcgagccga cccggttctc ggtcatgctg aagttcggcg gcatgcccaa ggtcctgtcc
     1201 atgccctaca cggccgtgac ccggttctac gatccctccg tccagttcct gcttcagttc
     1261 gaggccccga ccacggtcga ggaaatcgcc gctgaagtcg ccgctgcgga gcccgtgcgg
     1321 accgaccccg acggcaagga cgatggcccc aaggtcgtct ccctcgacca gttccggaag
     1381 aagtagcccc ttccccttcc ggcaacctcg gctaacctcg gcctgttcga agccggggga
     1441 acggtcatgg gacgggtgtt tgtcatcgta ctggcggtgt gggtcgccct tgtctcgccc
     1501 gcctgcgccc aagccgcgcg agcctcggcc ggccggttcg aaggctggac atccgcgatc
     1561 gtcgcggccg actggcgcga cggacgcggc cggccgatcc aggcctttga caatgcgcgc
     1621 cgcgacctgg tgaagggctt cctgaacgcc ggcttcccgc gcgcgaccat ggtcgactac
     1681 agcctgaggc ccgacgtccc gagtcccgcg acaccgcaga cggttctgga cggcctctat
     1741 gacgcggcgg cgcgagggag cgcgggctgt ctgctgtatt tcacctccca cggcgtgccg
     1801 acggccatgg tgttcggcga cgccccgcgc atgacccccg acatgatggt caatatcgtc
     1861 cggcgggcct gcggcgcgcg gccaaccgtc gtgatcgtct cggcctgcta ctcgggaatt
     1921 ttcgtcaacg ccctgtcagc gcccaaccgg atggtcctga ccgccgccag ccgggagcgg
     1981 acttcgttcg gatgcggcgc cgacgagacg tacccatggt ttgacggctg catactggag
     2041 acactgccga gggccgccga cttcctggcg ctggcggcgg gcgcccgggc ctgcgtggcg
     2101 cgcaaggagc aggagcacgc ggtcgaccta ccctcggaac cccaactgtt cgtcggatca
     2161 gagatgcagt tgcgcctgcc gacgctgcgg ttcaaccggc cggcggacgg gggaagaccc
     2221 ggatcgtgat ccgctcgcgt tcgccctcgc ggcgttccac cagcaggcgg gcgacctggg
     2281 cgtcgtcgtg gaacagatag cccttgatgg cgtccagcag ggccttggcc agattgtcca
     2341 gatccatcca cggcggatcg ccgacgtgct ccatcaggat ctcgaccgtg tagtcgccgt
     2401 atgacgggcg cgacgaccac tccttgcgca tgaactcata gaccagcggc ttgtagtatt
     2461 tggcctgggt ggtcaggcca tcgatagtga tctcgacggc gtctccgacc tcgcgggcgc
     2521 ggacctttcc gatgccgatc cagcctgcgg tcattcccaa gtcctggtcc cgagcgcctt
     2581 gttcgctcgc ggccccgtgc tacaccgccg cgcatcctcc cgccacgcct gacgagcccg
     2641 ttgccatgac gaaccgcacc gaaaccgaca ccttcggccc catcgaggtc gcctcggacc
     2701 gctactgggg cgcccaggcg cagcggtcgc tggggaactt caagatcggc tgggagaaac
     2761 agcccctgcc gatcgtgcga gccctgggca tcgtcaagcg cgcggcggca gagaccaata
     2821 tggcgctggg caagctggac ccggcgatcg ggaacacgat cgtcaaggct gccgacgagg
     2881 tcatcgccgg cacgctgaac gaccacttcc cgctggtggt ctggcagacc ggttcgggca
     2941 cccagtcgaa catgaacgcc aatgaggtga tctcgaaccg ggccatcgag a
//
LOCUS       DASVVN010000586         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_128338,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000586 DASVVN010000000
VERSION     DASVVN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128338"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF686_09580"
     CDS             <1..297
                     /locus_tag="VF686_09580"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8662105.1"
                     /translation="VRGFVVDFMTPETATSTHYFWGMARNFDIRDQGFTARFKAQQGG
                     VFTEDVEVLEAQQKAIEQNPDMRLKVFNIDAGGMRARTLIERRLKAQASVTTAP"
     gene            528..>2010
                     /locus_tag="VF686_09585"
     CDS             528..>2010
                     /locus_tag="VF686_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8662106.1"
                     /translation="MLTTGAHAQTPDTPGAEAPTQTPEPIIDAEDAQAMASEEEAEVE
                     EVVVTGTSIRGVAPAGSPVLAITPEQIQNNPATTATELLRQLPQVFNLGASDTQFTAS
                     NNANANVTGGSGINLRGLGTEATLTLVDSRRMPAAGTMAQYFDPSVIPTMAIGRIEVL
                     ADGGSAIYGSDAVGGVVNILLRRNFQGLELSARYGFADGLDQTVLGGVFGHKWSGGGL
                     MVAVEYNERSNLLASERDFYTDDLRPFGGTDQRSFNANPGNIQVGNTRYAIPAGQNGR
                     ALTPAQLIPGTANRRSAFFEADALPAQERLTSAFTFDHDLTDRISFSAQGIFAKREFV
                     RGQGNFTSNLTVPRSNPFFVHPTNPAAPNVTVNYSFNEDFGRSERVADSDFRFVTGNF
                     DVELGADWSATAYASHGEDTENSFASSVNTAALNRALADPNAATAFNPFGAGSNTPQA
                     TLDLIEARFRIASEYTLEEFGVTANGPVFTLPGGEVRAAIGLSR"
BASE COUNT          350 a          732 c          640 g          288 t
ORIGIN      
        1 gtgcggggct tcgtggtcga cttcatgacc ccggagacgg ccacgagcac ccattacttc
       61 tggggcatgg cgcgcaattt cgacatccgc gaccagggct tcaccgcccg cttcaaggcg
      121 cagcagggcg gggtgttcac cgaggacgtg gaagtgctcg aggcgcagca gaaggcgatc
      181 gagcagaacc ccgacatgcg cctcaaggtg ttcaacatcg acgccggcgg catgcgcgcg
      241 cgcaccctga tcgaacgccg gctcaaggcg caggcgtcgg tgacgaccgc gccgtaatgg
      301 ctggctactc agtgtcgcag actgtatata acgcaaacaa aaatgttgac agtcagggct
      361 gatcccctca gcgtcgaatg aacgaactgg atgcgctcga aggcgtccgg aagccgacat
      421 gacgagcgag gaaacgtccg atgacaccca ggcgcgctgc ggcgctcgaa cccgcttgcc
      481 ttgcgcggcg cagccttttc gccacgtccg ctgtcgcgct ggcgctcatg ctgacgaccg
      541 gagcccacgc gcaaacgccg gacacgcccg gcgctgaggc gcccacgcag acgcccgagc
      601 cgatcatcga cgccgaggac gcgcaggcga tggcgtccga ggaggaggcc gaggtcgagg
      661 aggtcgtcgt caccggcacc agcatccgcg gcgtcgcgcc ggcggggtcg ccggtgctgg
      721 cgatcacgcc cgagcagatc cagaacaacc ccgccaccac cgctaccgag ctgctgcgcc
      781 agctgccgca ggtgttcaac ctgggcgcca gcgacaccca gttcaccgcc tccaataacg
      841 ccaatgccaa cgtcaccggc ggctcgggca tcaaccttcg cggcttgggc accgaggcga
      901 cgctgaccct ggtcgactcc cggcgcatgc cggcggccgg caccatggcg caatatttcg
      961 atccgtcggt gatcccgacc atggccatcg gccgcattga ggtgctggcc gacggcggct
     1021 cggccatcta cggctcggac gccgtgggcg gggtggtcaa catcctgctc cgccgcaact
     1081 tccagggcct ggagctgtcc gcccgctacg gcttcgccga tggcctcgac cagacggtcc
     1141 tcggcggcgt gttcggccac aagtggagcg gcggcggcct catggtcgcg gtcgagtaca
     1201 acgagcgcag caacctgctc gcctcagagc gggacttcta caccgacgac ctgcgcccct
     1261 tcggcggcac ggaccagcgc tcgttcaacg cgaacccggg caacatccag gtcggaaaca
     1321 cgcgctacgc gatccccgcg gggcagaacg ggcgcgcgct gacgccggcg cagctcattc
     1381 ccgggacggc gaaccgccgc agcgccttct tcgaggccga tgcgctgccg gcgcaggagc
     1441 gcctgacctc cgccttcacc ttcgaccacg acctgaccga ccgcatctcg ttctccgccc
     1501 aaggcatctt cgccaagcgc gagttcgtgc gcgggcaggg caacttcacc tccaacctga
     1561 cggtgccgcg cagcaacccc ttcttcgtgc acccgaccaa cccggcggcg ccgaacgtga
     1621 ccgtcaacta ctcgttcaac gaggacttcg ggcgctccga gcgcgtggcc gattccgact
     1681 tccggttcgt caccgggaac ttcgacgtcg agcttggcgc cgactggtcg gcgacggcct
     1741 acgccagcca tggcgaggat accgagaatt ccttcgcttc ctcagtcaac accgccgcgc
     1801 tcaaccgggc gctggccgat cccaacgcgg ccacggcctt caaccccttc ggcgcgggca
     1861 gcaacacgcc gcaggccacg ctcgacctga tcgaggcgcg cttccgcatc gccagcgaat
     1921 acacgctcga agagttcggc gtcaccgcca acggcccggt gttcacgctc ccggggggcg
     1981 aggtgcgggc ggctatcggc ctctcgcggc
//
LOCUS       DASVVN010000587         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_258301,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000587 DASVVN010000000
VERSION     DASVVN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258301"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VF686_09590"
     CDS             complement(<1..795)
                     /locus_tag="VF686_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8662107.1"
                     /translation="MARPNDGSAGGTAVFESFIYRGGDPAHETLRPRPDQYLNPILQG
                     FYPDPSVTRVGEDYYLVTSTFSYFPGIPVFHSRDLVNWTQVGNAIDRPDQLDFGNLGL
                     SRGVFAPTIEHRDGVFYILNTCVDCGGNFLITAADPAGPWSDPVWLPDLEGGIDPGLF
                     FDEDGSAWIVNNGPPEGAPRYDGHRAIWIQRFDPAMKTTVGPRTVLVDGGVDPSARPI
                     WIEGPHIIRRGDWYYLICAEGGTAEGHSQVVLRSRRPDGPYEAWSGN"
     gene            1028..>1899
                     /locus_tag="VF686_09595"
     CDS             1028..>1899
                     /locus_tag="VF686_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEX8662108.1"
                     /translation="MTALVNDLPADWRQGVFLGRLSLPEGPTPVLVRRGRVVDISAFS
                     PTAAQAAAATDLRDAPGADLGDLESLELAPAWDDVGRSPRLLSPIDLQCVKASGVTFA
                     VSAVERVIEERARGDADKAQSIRAALAHRVGADLSAVKPGSEQAYALKDALIADGLWS
                     QYLEVAIGKDAEIFTKAPVLSSVGWGDLVGVRPDSAWNNPEPEVAIVCDPTGRPVGAT
                     LGNDVNLRDIEGRSALLLGKAKDNNASAALGPFVRLFDGSFGLDDVRSATVDLTVDGP
                     EGFHLDGSSSMSEI"
BASE COUNT          314 a          609 c          677 g          299 t
ORIGIN      
        1 attgcccgac caggcttcat agggaccgtc cggccggcgc gagcgcagca cgacctgcga
       61 atggccctcg gcggtgccgc cctcggcgca gatcagatag taccagtcgc cgcgccggat
      121 gatgtgcggg ccctcgatcc agatcgggcg cgccgatggg tcgacgccgc cgtcgaccag
      181 cacggtgcgc gggccgacgg tcgtcttcat cgctggatcg aaccgctgga tccagatggc
      241 gcggtggccg tcgtagcggg gcgccccttc gggcgggccg ttgttgacga tccaggcaga
      301 gccgtcctcg tcgaagaaca ggccggggtc gatgccgccc tcaaggtcag gcagccagac
      361 cggatcggac caggggcccg ccggatcggc ggccgtgatg aggaagttgc cgccgcagtc
      421 gacgcaggtg ttgaggatgt agaacacgcc gtcgcggtgc tcgatcgtcg gggcgaacac
      481 gccgcgagac aggccaagat tgccgaaatc cagttggtcc ggccggtcga tggcgttgcc
      541 gacctgggtc cagttgacga ggtcgcggct atggaagacc gggatgccgg gaaagtagct
      601 gaaggtcgag gtgaccagat aatagtcctc gcccacgcgg gttaccgagg ggtcgggata
      661 gaagccttgc aggatcgggt tcaggtactg gtccgggcgg ggccggaggg tttcgtgggc
      721 aggatccccg ccgcggtaga tgaagctctc gaacaccgcc gtcccgccgg cggagccgtc
      781 gttcggccgg gccatggcgt cgccgcagac cgcgccggcg aggatcagcg ccagaaccgg
      841 tccagccggt attttcgaca atctcgacat tcgtcgcccc acccggtcta tttgtccgag
      901 ttttaaggaa gaggcgaacc gaaacaagtc cggatcgtat gccaaagcgc ttgacagggg
      961 ggccggcgaa tagagatgat agcgctacca gacaagagct ccgcgaagac ggagacagcg
     1021 ggggaagttg acggctcttg tgaatgattt gccggccgac tggcgtcagg gcgtgttcct
     1081 gggccggttg agtctcccgg aaggaccgac ccccgttctg gtccgcaggg ggcgcgtggt
     1141 cgatatatcc gccttctccc cgaccgcggc gcaggcggcg gccgcgaccg atttgcgcga
     1201 cgcccccgga gcggacctcg gggacctgga atcgctggaa ctcgccccgg cctgggacga
     1261 cgtcggccgt tcgccgcgcc tgctgtcgcc gatcgatctt caatgcgtga aggcgtcagg
     1321 cgtgaccttc gccgtctcgg ccgttgagcg tgtcatcgag gagcgggccc gcggcgacgc
     1381 cgacaaggcc caatcgatcc gcgccgccct ggcccaccgc gtcggcgcag acctgtcagc
     1441 ggtgaagccg ggctcggaac aggcctatgc gctcaaggac gccctgatcg ccgacggact
     1501 gtggtcgcag tatctggaag tcgccatcgg caaggacgcc gagatcttca ccaaggcccc
     1561 tgtcctgtcg tcagtgggtt ggggcgatct ggtcggcgtc cggcccgaca gcgcctggaa
     1621 caatcccgag cccgaggtgg ccatcgtctg tgatccgacg ggccggccgg tcggcgcgac
     1681 cctgggcaac gacgtcaatc tgcgcgacat cgagggccgt tcggccctgc tgctgggcaa
     1741 ggccaaggac aacaacgcct cggccgcgct ggggccgttc gtgcggttgt tcgacgggag
     1801 cttcggcctc gacgacgtgc gctccgcgac cgtcgacctc accgtcgacg gcccggaggg
     1861 gttccacctc gacggcagct cgtccatgtc ggagatcag
//
LOCUS       DASVVN010000588         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_311658,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000588 DASVVN010000000
VERSION     DASVVN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311658"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF686_09600"
     CDS             <1..1030
                     /locus_tag="VF686_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX8662109.1"
                     /translation="WGLVVTKGPSEDREDDFNLGIYRMQVLGRDRTIMRWLAHRGGAQ
                     HYARHKKSGARDPLPAAVVLGADPGTILAAVTPVPDTLSEYQFAGLLRGQKAELVPCK
                     TIPLMVPANAEIVLEGHVLFDEHAPEGPYGDHTGYYNSVETFPVFQVTAITMRRDPIY
                     LTTFTGRPPDEPSVLGEALNEVFIPLLRQQFPEITDFWLPPEGCSYRIAVVSMKKAYP
                     GHAKRVMLGVWSYLRQFMYTKWVIVVDDDIDARDWKQVMWAISTKMDPARDITVIENT
                     PIDYLDFASPESGLGSKIGLDATDKWPPETKREWGEEIRMDEAVVSRVSEIWETLGLP
                     GDGTPIWK"
     gene            1144..1491
                     /locus_tag="VF686_09605"
     CDS             1144..1491
                     /locus_tag="VF686_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662110.1"
                     /translation="MKSVLVIAAAIVMAMPVVSVPTVADAQVLTGRGGRAAPARRARP
                     APPRLSEAEENQLWDAQSAIDTLDGEVADLRAAGEAQGGLTAEQQAYLNAQAARRTEL
                     EAVVTRLEAKKNR"
     gene            complement(1502..>2165)
                     /locus_tag="VF686_09610"
     CDS             complement(1502..>2165)
                     /locus_tag="VF686_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8662111.1"
                     /translation="LLAALPPERQAAAERLAGRFHLDPVGWYQSADEAVRLPAIAQAV
                     WTSRRIAVRYESWKGEVARELEPLGLILKAGLWYLAARPADGKQREPRTYRVSNILDL
                     SVGEATFERPAGFDLAAWWSETSRRFEAEIFTGAATLRLSREGRRRLARLGVEQARIA
                     AQADAAAGEGVAEVVVPIESIEHAATDFQRLGAEAEVLAPAALRSQMRDAALRLAGLY
                     RD"
BASE COUNT          365 a          783 c          693 g          324 t
ORIGIN      
        1 ctggggtctg gtcgtcacca aggggccgtc cgaggaccgc gaggacgact tcaacctcgg
       61 catctaccgc atgcaggtgc tggggcgcga ccgcaccatc atgcgctggc tggcgcaccg
      121 cggcggcgcc cagcactacg cccgccacaa gaagagcggc gcgcgcgatc cgctgcccgc
      181 ggcggtggtg ctcggcgccg acccgggcac catcctcgcc gccgtcacgc ccgtgccgga
      241 caccctgtcg gagtaccagt tcgccggcct gctgcgcggc cagaaggccg aactggtccc
      301 gtgcaagacc atcccgctga tggtcccggc aaacgccgag atcgtgctgg aaggccacgt
      361 cctgttcgac gaacatgcgc cggagggccc gtacggcgac cacaccggct actacaactc
      421 ggtcgagacc ttcccggtct tccaggtcac cgccatcacc atgcgccgcg atccgatcta
      481 tctgaccacc ttcaccggcc gcccgccgga cgagccctcg gtgctgggcg aggcgctgaa
      541 cgaggtcttc atccccctgt tgcgccagca gttccccgag atcaccgact tctggctgcc
      601 gccagagggc tgttcctacc gcatcgccgt ggtgtcgatg aagaaggcct acccgggcca
      661 cgccaagcgg gtgatgctgg gcgtctggag ctatctgcgc cagttcatgt atacaaagtg
      721 ggtgatcgtc gtggacgacg acatcgacgc ccgcgactgg aaacaggtca tgtgggccat
      781 ctcgaccaag atggatcctg cgcgcgacat caccgtcatc gagaacaccc cgatcgacta
      841 cctggacttc gcctcgcccg agagcggtct cggctccaag atcggcctcg acgccaccga
      901 caaatggccg cccgagacga agcgcgaatg gggcgaggaa atccgcatgg acgaggcggt
      961 ggtctcgcgc gtgtcggaaa tctgggaaac gctcggattg ccgggtgacg gaacgccgat
     1021 ctggaagtga tgcctcgaag cgacgtcggc cgaagcgcct tcgattgtga ccaaaaagcg
     1081 ctttccagat gacgaacttg taatctaacg tcgcggttcg gccttaccca tggaaccgtc
     1141 acaatgaaat ctgttctcgt catcgccgcc gctatcgtga tggccatgcc cgttgtgtcg
     1201 gttcctaccg tcgctgatgc gcaggttctc actggccggg gcggccgcgc cgcgcccgct
     1261 cgccgcgccc gcccggcccc gccgcgcctc agcgaggccg aggaaaacca gctgtgggac
     1321 gcccagagtg caatcgacac ccttgacggc gaggtcgccg accttcgggc cgcgggcgaa
     1381 gcgcagggcg gcctgaccgc cgagcagcag gcttatctga acgcacaggc cgcgcgccgc
     1441 accgagcttg aagccgtcgt cacgcggctc gaggccaaga agaaccgctg atcgcggcgc
     1501 ttcagtcccg gtagagtccc gcaagacgca gggcggcgtc ccgcatctgc gaccgcaaag
     1561 ccgccggagc cagcacctcg gcctcggcgc ccagccgttg aaaatccgtg gcggcgtgct
     1621 cgatcgactc tatcgggacg accacctccg ccacgccttc tccggccgcc gcatcggcct
     1681 gtgcggcgat ccgcgcctgt tccacgccga gccgcgccag ccgtcggcgg ccctcgcgcg
     1741 acagccgcag cgtcgccgcg ccggtgaaaa tttccgcctc gaaacggcgc gaggtttccg
     1801 accaccaggc ggccagatcg aacccggcgg gccgctcgaa cgtcgcctcg cccacgctca
     1861 ggtccagaat gttggagacg cgataggtgc gcggctcccg ctgttttcca tctgccggac
     1921 gcgccgccag ataccagagc ccggccttga ggatcaggcc cagcggctcc agttcgcgcg
     1981 ccacctcgcc cttccagctc tcatagcgca cggcgatgcg ccggctggtc cagacggcct
     2041 gggcgatggc gggcaggcgc acggcctcgt cggcgctctg gtaccaaccg accggatcga
     2101 gatggaaccg gccggccagc cgttcggccg ccgcctgccg ttcgggcggc agggccgcca
     2161 gcagt
//
LOCUS       DASVVN010000589         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_128456,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000589 DASVVN010000000
VERSION     DASVVN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128456"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..926)
                     /locus_tag="VF686_09615"
     CDS             complement(78..926)
                     /locus_tag="VF686_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8662112.1"
                     /translation="MRWQGGRRGGNIEDRRGMGGGAMAGGGIGALVLAAIGYFVFGID
                     PQTTTQLASQFGGVGAAEQGKIGSPEDQAGAFVDVVGSNVDDVWKTKLQGYQPPTVTL
                     YEQGTSTGCGFGQSAMGPFYCPNDRKVYLDLGFWRDMETQLGASGADFARAYVIAHEY
                     GHHVQTLTGASQQVQEAQQRASGQAEANRYSVALELQADCYAGVWAANAAAVSNGEVA
                     VDAADLEAGMQTASAIGDDTLQRRQGGRVQPDGFTHGSSAQRVEWLRRGYESGDPAAC
                     DTFGSL"
     gene            complement(990..1394)
                     /locus_tag="VF686_09620"
     CDS             complement(990..1394)
                     /locus_tag="VF686_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662113.1"
                     /translation="MWGHLLRMNATGPLIAVLILVAAPVQAQVPQVRPGLSTPDLHRY
                     QADQHRYEIDRLRAQADQREMNARLHQTETRLNRLRIEAARQPEPVQPPPYRALRSPE
                     EERALRLSAEARNRGMAQGVGQIDAWLDRQPD"
     gene            complement(1429..2091)
                     /locus_tag="VF686_09625"
     CDS             complement(1429..2091)
                     /locus_tag="VF686_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8662114.1"
                     /translation="MSRIADPAAFIAANTRLQPVPHAPEISLWLADEVTPIWRLTEEE
                     LGEMGLPPPFWAFAWAGGQGLARWLMDNPAEVSGKRVLDFAAGSGLVGIAAMKAGAAS
                     ALCADIDPFCGAAVARNADANGVTLDFTAADLLDAPPPEVDLICAGDVFYEQPMSSRV
                     LDWLRLAADHGARVLVGDPLRTYFPREGFDLLAEYAVPTTRELEDSAVKRTRVWTLSS
                     AD"
     gene            complement(2088..2516)
                     /locus_tag="VF686_09630"
     CDS             complement(2088..2516)
                     /locus_tag="VF686_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8662115.1"
                     /translation="MGKMIPDPTEDGMTASQRIDAKIASFDDWRGQTLARIRAIIHQA
                     DPEVVETVKWGRVPVFEHDGIITTAETYKTAVKMTFAKGASLEDPKGLFNSSLDGNTR
                     RAIDFHEGDKIEEEALKALIHAAVALNVSSHAAKRRSGAA"
     gene            complement(2531..2959)
                     /locus_tag="VF686_09635"
     CDS             complement(2531..2959)
                     /locus_tag="VF686_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8662116.1"
                     /translation="MAGKPDNTAEPILLSGGNPQIAKGYGDAPIQAWIAAAPGWKSAA
                     AHRLDALIERTVPGVKKAVKWNTPMYGLEEGSWFVSFHAFDRYLKLAFFRGASLDPVP
                     PVGSKQKDVRYFHIHEDDPVDEAQLADWIEQASRLPGERM"
BASE COUNT          470 a          996 c         1062 g          523 t
ORIGIN      
        1 ctttgacaat cgaatacaat gacagacggc gtccaagcga agtggactca gtggactctt
       61 cggactgtgt tttttggcta gagcgacccg aaggtgtcgc aggccgcggg gtcgccggat
      121 tcatagccgc gccgcagcca ctcgacccgc tgggccgagg agccgtgggt gaagccgtcc
      181 ggctggaccc gtccgccctg acggcgctgc agggtgtcgt ccccgatggc cgaggcggtc
      241 tgcatgccgg cctccagatc ggcggcgtcg accgcgacct cgccattgga aaccgcggcg
      301 gcgttggccg cccagacccc ggcgtagcag tcggcctgca gctccagcgc caccgagtag
      361 cggttggctt ccgcctgacc cgaggcccgc tgctgggcct cctgaacctg ctgcgaggcg
      421 ccggtcaggg tctggacgtg gtggccatac tcgtgggcga tgacataggc ccgggcgaag
      481 tcggccccgg aggcgcccag ctgggtctcc atgtctctcc agaagcccag gtcgagataa
      541 accttgcggt cgttggggca gtagaacggc cccatggcag actggccgaa gccgcagccg
      601 gtcgaggtgc cctgttcgta cagggtaacg gtcggcggct gatagccctg cagcttcgtc
      661 ttccacacgt cgtcgacatt ggagccgacc acgtcgacga aggcgcctgc ctgatcctcg
      721 gggctgccga tcttgccctg ttcagccgcg ccgacgccgc cgaactggct ggccagctgg
      781 gtggtggtct gcggatcgat gccgaagacg aaatagccga tggccgcaag gaccagggct
      841 ccgatgcctc cgccggccat ggcgccgccg cccatgccgc gtcgatcctc gatattgccg
      901 ccgcgccgtc cgccctgcca ccgcatgttc gttctcccgt ccctcgttga agctaggccg
      961 gaacgcagag ggaagggaag ggatgcaggc tagtccggct gacggtccag ccaggcgtcg
     1021 atctggccga cgccctgcgc catgccccgg ttgcgcgcct cggcggagag gcgcagcgcc
     1081 cgttcctctt ccggcgaacg cagggcccgg tacggcggcg gctggacggg ttcgggctgg
     1141 cgcgccgcct cgatgcggag ccggttcagg cgggtttccg tctggtgcag gcgggcgttc
     1201 atctcgcgct gatcggcctg tgcgcgcaga cggtcgatct cgtagcggtg ctggtcggcc
     1261 tgatagcggt gaagatcggg cgtggagagg ccggggcgca cctgtggaac ctgcgcctga
     1321 acgggagcgg cgacgaggat gaggactgcg atgaggggcc cggtggcgtt catgcggaga
     1381 agatggcccc acatgggcct gtctccaagc gtggctgcaa ccgccggatc aatcggcgct
     1441 ggacagggtc cagacgcggg tgcgtttgac ggcggaatct tcgagttcgc gtgtggtcgg
     1501 aaccgcgtat tcggccagca ggtcgaagcc ctcccttggg aaataggttc gtaagggatc
     1561 accgacgagg acgcgcgccc cgtggtcggc tgcgaggcgc agccagtcca gaacccggct
     1621 cgacatcggc tgttcataga agacgtctcc cgcacagatg agatcgacct cgggcggcgg
     1681 ggcgtccagc agatcggcgg cggtgaagtc cagggtcacg ccgttggcgt cggcgttgcg
     1741 cgccaccgcc gccccgcaaa agggatcgat gtcggcgcac agggccgagg ccgcgccggc
     1801 tttcatcgcg gcgatcccga cgaggcccga gccggctgcg aaatccagca cccgtttgcc
     1861 agagacctcg gccggattgt ccatcagcca gcgcgccagc ccctgaccgc ccgcccaggc
     1921 gaaggcccag aacggcggcg gcaggcccat ctcccccagc tcctcctccg tcaatcgcca
     1981 gattggcgtg acctcgtccg ccagccacaa cgatatctcc ggcgcatgcg ggaccggctg
     2041 caggcgggtg ttggcggcga tgaaggcggc ggggtcggcg atgcggctca tgccgcgccg
     2101 gatcggcgct tcgcagcgtg cgacgacacg ttcagggcca cggctgcatg gatgagcgcc
     2161 ttcagcgcct cttcctcgat cttgtcgccc tcatggaagt cgatggcgcg ccgcgtattg
     2221 ccgtcgaggc tggagttgaa cagccccttg gggtcctcaa gcgaggcgcc cttggcgaag
     2281 gtcatcttga cggcggtctt gtaggtctcg gcggtggtga tgatgccgtc gtgttcgaac
     2341 accggaacgc ggccccattt caccgtctct accacctcgg ggtcggcctg atggatgata
     2401 gcgcggattc gggccagcgt ctggccgcgc cagtcgtcga acgaggcgat cttcgcatcg
     2461 atccgctggg aggcggtcat gccgtcttca gtaggatcgg ggatcatctt tcccatgggt
     2521 ctgtcgcctt tcacatgcgt tcgccgggca gccggctggc ctgttcgatc cagtcggcca
     2581 actgggcttc gtcgaccgga tcgtcctcgt ggatgtggaa atagcgcacg tccttctgct
     2641 tcgagcccac aggcggcacg ggatcgagcg aagcgccccg gaagaaggcc agcttcagat
     2701 agcggtcgaa ggcgtggaag ctgacgaacc aactgccttc ctcaaggccg tacatcggcg
     2761 tgttccattt gacggccttc ttcacgccgg ggactgtgcg ttcgatgagg gcgtccaggc
     2821 gatgtgcagc cgcgctcttc cagccgggcg cggccgcgat ccaggcctga atcggagcgt
     2881 cgccatagcc ctttgcaatc tgcggattgc cgcccgacag caggatgggc tccgcggtat
     2941 tgtccggctt tccggccatc gtcttcacgc cgatccgcgc ccgccgcgcg atttgacggc
     3001 ttagggcagc gcgaacatgt acaggccggt cagggtgagc accgcgagcg g
//
LOCUS       DASVVN010000590         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_205518,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000590 DASVVN010000000
VERSION     DASVVN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205518"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2221
                     /gene="alaS"
                     /locus_tag="VF686_09640"
     CDS             <1..>2221
                     /gene="alaS"
                     /locus_tag="VF686_09640"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX8662117.1"
                     /translation="GYTARHLTFFEMLGNFSFGDYFKEHAIDRAWNLVTKDYGLDPKR
                     LLVTVYIDDDEAAAIWKKVTGFSDDKIIRIAGSDNFWAMGDNGPCGPCTEIFWDYGDH
                     VPGGPPGSPDEDGDRFVEIWNNVFMQFEKENDEIVRSLPKPSVDTGMGLERIASVLQG
                     KNSVFDTDLFQTLIAASADATSTDPNGDMAASHRVIADHLRSSSFLIADGVTPSNEGR
                     GYVLRRIMRRAMRHAHLLGASDPLMHRLVPTLVSEMGEAFPELKRAQAFVEDTLKQEE
                     IRFRTTLGRGMGLLEDASRDLGEGGVLSGQTAFTLYDTYGFPLDLTQDEARRRGFTVD
                     TDAFDAAMNEQKARSREHWTGSGQTASTGEWLALRDRLGPTVFTGYDAVEGSGEVLAI
                     VRGGASVDELAPGETAEVLFDQTPFYGEGGGQAGDRGEIEWTDATGQQGSATVLDVQK
                     HAGDLFAHRIEVTSGTLAIGARAQLRSSAAARLTTRANHSAAHLLHKALANVLGGHVA
                     QKGQMVDAERLRFDFSHNAPVTEDELARMEDEVNAVIRQNLPAETKMMGPQEAIAAGA
                     VALFGEKYGDEVRVLTLGRALDGQGPYSVELCGGTHVARTGDIALFKITSETGIAAGV
                     RRIEALTGEAARQYLLAQAGVARTLAQSFKVQPLDVPARVEALVADRRTLEKQVADLK
                     KQLALGGGGSANAAPEEINGVKLIARVLDGVDGKGLRGVAEDFRKQVGSGVVALIGLT
                     "
BASE COUNT          366 a          826 c          710 g          319 t
ORIGIN      
        1 tggctacacc gcccgacacc tgaccttctt cgagatgctg gggaatttct ccttcggcga
       61 ctatttcaag gaacacgcga ttgaccgcgc gtggaacctc gtcaccaaag actacggcct
      121 cgatccgaag cgtctgctgg tcaccgtcta catcgatgac gacgaggccg cggcgatctg
      181 gaagaaggtc accggcttct ccgacgacaa gatcatccgc atcgccggtt cggacaattt
      241 ctgggccatg ggcgacaacg gcccgtgcgg tccatgcacc gagatcttct gggactacgg
      301 cgaccacgtc cccggcggac ctcctggttc accggacgag gacggcgacc ggttcgtgga
      361 gatctggaac aacgtcttca tgcagttcga gaaggagaac gacgagatcg tccgctccct
      421 tcccaagccc agcgttgaca ccggcatggg gctggagcgc atcgcctcgg tgctgcaggg
      481 caagaactcg gtcttcgaca ccgacctgtt ccagaccctg atcgccgcct cggccgacgc
      541 cacctcgacc gacccgaacg gcgacatggc cgccagccac cgggtcatcg ccgaccacct
      601 gcgttcctcg tccttcctga tcgcggacgg cgtcaccccc tcgaacgagg gccgcggcta
      661 cgtcctgcgc cgcatcatgc gccgcgccat gcgccacgcc cacctgctcg gtgcctcgga
      721 cccgctgatg caccggctgg tgccgacgct cgtctccgaa atgggcgagg ccttccccga
      781 gctgaagcgc gcccaagcct tcgtcgagga cacgctcaag caggaagaga tccgcttccg
      841 cacgacgctt ggccgcggca tgggcctgct ggaggacgcg tcgcgcgacc tcggcgaggg
      901 cggcgtgctg tcgggccaga cggccttcac cctgtacgac acctacggct tcccgctcga
      961 cctgacccag gacgaggcgc gccgccgcgg cttcactgtc gacaccgacg ccttcgacgc
     1021 cgccatgaac gagcagaagg cccgcagccg cgagcactgg accggctcgg gccagaccgc
     1081 ctcgaccggc gaatggctgg ccctgcgcga ccgcctcggc cccaccgtct tcaccggcta
     1141 tgacgccgtc gagggttcgg gcgaggtcct ggccatcgtg cgcggcggcg cctccgtcga
     1201 cgaactggcg cccggcgaga ccgccgaggt cctgttcgac cagaccccct tctacggcga
     1261 aggcggcggt caggccggcg accgcggcga gatcgaatgg accgacgcca cgggccagca
     1321 gggctcggcc acggtgctgg acgtccagaa gcatgccggc gacctgttcg cccaccgcat
     1381 cgaggtgacc tcgggcacgc tggccatcgg cgcccgcgcc cagcttcgct ccagcgccgc
     1441 cgcccgtctg accacccgcg ccaatcactc cgccgcccac ctgctgcaca aggcgctcgc
     1501 caatgtgctg ggcggccacg tggcgcagaa gggccagatg gtcgacgccg aacgcctgcg
     1561 tttcgacttc agccacaacg cccccgtcac cgaggacgag ctggcccgga tggaggacga
     1621 ggtgaacgcc gtcatccgcc agaacctgcc cgccgagacg aagatgatgg gtcctcagga
     1681 agccatcgcc gccggcgccg tcgccctgtt cggcgagaaa tacggcgacg aggtccgggt
     1741 cctgaccctc ggccgcgccc tcgacggcca aggcccctat tcggtcgagc tgtgcggcgg
     1801 cacccacgtg gcccgcaccg gcgacatcgc cctgttcaag atcacctccg agacgggcat
     1861 cgccgccggc gtgcgccgca tcgaggccct gaccggcgag gccgcccgcc agtatctgct
     1921 ggcccaggcc ggcgtggcca ggaccctcgc ccagtccttc aaggtccagc cgctggacgt
     1981 ccccgcccgc gtcgaggcgc tggtcgccga ccgccggacg ctcgagaaac aggtcgctga
     2041 tctgaagaag cagctggccc tcggcggcgg cggttcggcc aacgccgcgc ctgaagagat
     2101 caacggcgtc aagctgatcg cccgcgttct ggacggcgtc gacggcaagg gcctgcgtgg
     2161 cgtggctgag gacttccgca aacaggtcgg ctcgggcgtc gtagccctga tcggcctgac
     2221 c
//
LOCUS       DASVVN010000591         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_182365,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000591 DASVVN010000000
VERSION     DASVVN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182365"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..757
                     /locus_tag="VF686_09645"
     CDS             11..757
                     /locus_tag="VF686_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8662118.1"
                     /translation="MRSLAAAALLACSVAALGGCAYNETLGRNQLLIVDDSSLAQQSD
                     AAWAEALRTQRVSSDAAGNARIRRVGDKIVQAAGLTDRRWEYALFVSDSPNAFVLPSG
                     KIGVTTGLLALVRNDDQLATVLGHEVGHVVARHAAERYSTTALTNIGLSGVQSAAGDY
                     GRAAGAIGGLGAQLGLLLPFSRSHELEADRLGVDYLQRAGYRPSEAVALWRLMAQQRQ
                     GSNPEFASTHPSDSTRIAALEQYIASRGWS"
     gene            839..915
                     /locus_tag="VF686_09650"
     tRNA            839..915
                     /locus_tag="VF686_09650"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:873..875,aa:His,seq:gtg)
     gene            945..1511
                     /locus_tag="VF686_09655"
                     /pseudo
     CDS             945..1511
                     /locus_tag="VF686_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(1630..3480)
                     /locus_tag="VF686_09660"
     CDS             complement(1630..3480)
                     /locus_tag="VF686_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8662119.1"
                     /translation="MAPARSDRLEDALHRIFEGGKNTRASWFALTGGETLFAEGDGAD
                     TLYLLRSGRLGVFRQDPNEDRPPQFVGVIRPGEPAGEMAMLAGTVHTADVVALRDCEV
                     LALPRDAFFEAARTEPDLMVELSRLMIQRARDRRSGGGEPSVFGFISARPRPIRAFVE
                     RIAAAIEADGFSCQVIDHSALASAAEWFSRVEDAHDFVLYVAEQDEPAWAALCARQVD
                     RVFVVGNGLNAPPNRPLPHAGVLDAQRRTDLILLRDPRMLRPANTAVWLDSIQPGRWF
                     QCVEGDQSDADRMARVVTGSAVGVVMSGGGARAYSHIGALRALKEAGVPIDFVGGSSM
                     GAVIAAGPALGWTQEELEERIRKAFVESDPLSDLTFPVLAMTRAKKVERLLLQAYGEI
                     DLADLQRPFFAVSTNLTSGRIEVHRRGLMRQAIRASIAIPGLLPPIVMDGQVLVDGAV
                     LKNLPTEVMRQMHGGPIIAVDMSEARGVDPDLIEHPPSIWRLIATGAWRRGPPIVSIL
                     MRSATLTTDADIESSRAATDILIQPTPDGLDIRDWKGFDLGVAAGYQATHEVLSLLDR
                     PVTALRRRRSVNDDAQPETAVQKAAASLRPGVLEKLRQGARRGRKEKPRV"
BASE COUNT          514 a         1220 c         1236 g          556 t
ORIGIN      
        1 gagacgcccc atgagatcgc tcgccgccgc cgccctgttg gcctgttccg tcgccgccct
       61 cggcgggtgc gcctacaatg agaccctcgg ccggaaccag ttgctgatcg tcgacgacag
      121 ttcgcttgcg caacagtccg atgcggcatg ggcggaggct ctgcgcaccc agcgcgtttc
      181 ttccgacgcc gccggcaacg cccgcatccg ccgggtcggc gacaagatcg tccaggccgc
      241 gggcctgacc gaccgccgct gggaatacgc cctctttgtc agcgacagcc ccaacgcctt
      301 tgtccttccc tccggcaaga tcggcgtgac cacgggtctg ctggccctgg tccgcaacga
      361 cgaccagctg gcgacggtcc tcggccatga ggtcggccat gtggtcgcgc gccacgcggc
      421 ggaacgctat tcgaccaccg ccctgaccaa tatcggcctg agcggggttc agagcgccgc
      481 gggcgactat ggccgcgcgg cgggcgccat cggcggtctg ggcgcccaac tcggcctcct
      541 tctgccgttc tcgcgcagcc acgagctgga agcggatcgg ctgggcgtcg attatctgca
      601 gcgtgccggc taccgcccct cggaggccgt ggcgctgtgg cggctgatgg cgcagcagcg
      661 tcaggggtcc aatccggaat tcgcctcgac ccatccgtcc gactccacgc ggatcgccgc
      721 gctggagcag tacatcgcca gccgcggctg gagctgaagc gtccgcctgc gaatattccc
      781 gcccggtcgc ttgatcgggc ggagctgtcc cggtagatac cgccctcgcg ctgattgcgc
      841 cgccttagct cagttggtta gagcgccggt ttgtggaacc ggaggtcctc agttcaagcc
      901 tgagaggcgg taccatcctc accttggcgg agggcgtttc cggcatgacc gacacgtccc
      961 gtccgcggcc cgaaacgggc tgggatccca ggcagtatca acgtttcgag gccgagcgcg
     1021 accgcgccgc ccaggacctg ttggcgcgcc tgccggctga cctgacaccg gccgagatct
     1081 gggatctggg ctgtggcgcc ggccagcacg cgatccagct gcaccgccgc catccccgtg
     1141 ccgccgtcca tggcctggat tccagcgccg ccatgctgga tcaggcgcgg gcggcggggc
     1201 cggatatcga ctggcggctg ggcgatctgg ccgactggcg tcccgaagtc ccggcggacc
     1261 tgatcttcgc caacgcctcg ctgcagtggg tcccggatca cgccgcgctg tttccccggc
     1321 tgatacaggc gctggcgccc ggcggcgtca tcgccgtgca gatgccgatg gcgcatgaga
     1381 gcctccagca cgccctgatg cgcgaggtcg cggcgcaggg gccgtgggcc ggcgaccttg
     1441 ccgggctcgg tacgatcggg ccgctgctgt ccgccgaggc ctattacgac ctgctggccc
     1501 gtcattgcgc cgggctcgac atctggtcga cgcgctatct gcacgtcctc ggcgggatcg
     1561 atcctgtgct tgaatggatg aaggggaccg ctctgcggcc ctacctgtcc gcccttcagg
     1621 acgacctggc taaacgcgag gcttttcttt ccgccctcgg cgcgcgcctt gccgaagctt
     1681 ttccagcacg cccggacgga gcgacgctgc tgccttttgc acggctgttt ctggttgcgc
     1741 gtcgtcgttg acagaccggc gccggcgcag ggctgtcacg ggacggtcca gcagggacag
     1801 cacctcgtgg gtggcctgat agccggcggc cactcccagg tcgaaaccct tccagtcgcg
     1861 gatatccagg ccgtccggcg tgggctgaat caggatgtcg gtcgccgcgc gcgagctctc
     1921 gatatccgca tctgtggtca gggtcgccga ccgcatcagg atcgagacga tcggcgggcc
     1981 tcgccgccac gccccggtcg cgatcagccg ccagatcgac ggcggatgct cgatcaggtc
     2041 cgggtccacg ccgcgcgcct ctgacatgtc gacggcgatg atcggtccgc cgtgcatctg
     2101 gcgcatcacc tcggtcggca ggttcttcag caccgcgccg tcgaccagca cctgaccgtc
     2161 catgacgatc ggcggcagca ggccgggaat ggcgatcgag gcgcggatgg cctgacgcat
     2221 cagaccgcgc cgatgcacct cgatccggcc gctggtcagg ttggtggaca cggcgaagaa
     2281 cggacgctgc agatccgcca gatcgatctc gccataggcc tgaagcagca ggcgttcgac
     2341 cttcttcgcc cgggtcatgg ccagcacggg gaaggtcagg tccgacaggg ggtcgctttc
     2401 gacgaacgcc ttgcggatgc gctcttccag ctcttcctgg gtccagccga gggctggccc
     2461 ggcggcgatc accgccccca tcgatgagcc gccgacgaag tcgatgggca cgcccgcttc
     2521 cttcagtgcc cgcagggcgc cgatgtggga ataggcccgc gcccctccgc ccgacatgac
     2581 gactccgacg gccgagccgg tgacgacccg cgccatcctg tccgcgtccg attgatcgcc
     2641 ctcgacgcac tggaaccagc ggcccggctg gatggagtcc agccagacgg cggtgttggc
     2701 gggccgcagc atgcgcggat cgcgcagcag gatcagatcc gtgcgccgct gggcgtccaa
     2761 caccccggcg tggggcaggg gccggttggg cggtgcgttc aggccgttgc cgaccacgaa
     2821 cacccggtcg acctgacgcg cgcacagggc ggcccaggca ggctcgtcct gttcggcgac
     2881 gtagaggacg aagtcgtggg cgtcctcgac ccggctgaac cattccgccg ccgaggccag
     2941 ggccgaatgg tcgatgacct gacagctgaa gccgtcggcc tcgatggcgg cggcgatgcg
     3001 ttcgacaaag gcgcggatcg ggcggggccg ggccgagatg aagccgaaca cgctgggctc
     3061 gccgccgccc gatcggcggt cgcgggcgcg ctggatcatc agccgggaca gctcgaccat
     3121 caggtcgggc tcggtgcggg cggcctcgaa gaaggcgtcg cgcggcaggg ccagaacctc
     3181 gcagtcgcgc agggcgacga catcggcggt gtggacggtg ccggccagca tggccatttc
     3241 gccggcgggt tcgccgggcc ggatgacgcc gacgaactgg ggtgggcgat cctcgttcgg
     3301 atcctggcgg aagacgccaa ggcggccgct gcgcagcaga tacagggtgt cggccccgtc
     3361 cccctctgcg aacagggtct cgccgcctgt cagggcgaac cagctggcgc gcgtattctt
     3421 gccgccctcg aagatgcggt gcagggcgtc ttcgagccgg tcggagcggg caggggccat
     3481 ggacggacgt tagagggagt cgcgggcggc ggtagagaga agtggg
//
LOCUS       DASVVN010000592         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_417981,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000592 DASVVN010000000
VERSION     DASVVN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417981"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VF686_09665"
     CDS             <1..387
                     /locus_tag="VF686_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX8662120.1"
                     /translation="RLGREVELIRDFVILHYHATQRDDSAFWRQVAAMPIPDTLAERV
                     EAFRERALLYQVGADEYFSQASWLAVMYGQGIIPRGPNPLYAYQDADRSAAGLRQLSE
                     ILARTVEGLPDHEAYLRANAMWSGDA"
     gene            complement(384..974)
                     /locus_tag="VF686_09670"
     CDS             complement(384..974)
                     /locus_tag="VF686_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662121.1"
                     /translation="MRRRAGLLGLLLALIAAPAWAAVTLTFYAHPGARIRGGELLFPH
                     AYVHATGTLDDTGDAVDWAAGFTAKYPGPQLLLATGPGIVATPEARYVGEGKPYLRIT
                     VSDAVYRAVRARSDWWNGPEGSLYDLRRRNCISYIADLARVAGLQTAREPSMKPGTFL
                     EATAALNPAAAWRETPEPAVPAPAIPVQPLEPSPAV"
     gene            complement(1085..>2290)
                     /gene="gluP"
                     /locus_tag="VF686_09675"
     CDS             complement(1085..>2290)
                     /gene="gluP"
                     /locus_tag="VF686_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HEX8662122.1"
                     /translation="TSLIGPLIAAVRRVFELSYTEATLTTFAWFIAYGIASIPAAAIL
                     GRLGYSRAIIVALVTMVAGCLIVPIATLADFYPGVLIALFVIASGVTLLQVGANPLVA
                     ALGAQKSSHARLNFSQFFNSLGTTLGPLIAAPILLTGGVFAADAVITDPASRAESLRS
                     IDLAFLAVGVFFAVVAGFIFSARKTINAAAPAPSADDRMSPLVALKSPWAVFGALAIF
                     FYVGSEVTIGDLLTNFLHSPQILNIPLAAAGSMVAYYWGGAMVGRLVGGLLMLTKIPA
                     ATILIVNTVVAATLSLVVTQTSGTTAAYAAIAIGFFNSIMFPTIFTLTLERSTAPVPA
                     TSGLLVFGIIGGAVLPPVAGQIADNAATLNPVFYVPLVGYLLLVGFAIAASRAKTRNT
                     AAAATPGGH"
BASE COUNT          387 a          770 c          814 g          319 t
ORIGIN      
        1 cgcctcggcc gcgaggtcga gttgatccgg gacttcgtca tcctgcacta ccacgcgacc
       61 cagagggacg attccgcctt ctggcgtcag gtggcggcca tgccgatccc cgacaccctc
      121 gccgagcggg tcgaggcctt ccgcgagcgg gcgctgctgt atcaggtcgg ggccgacgaa
      181 tacttcagcc aggcctcatg gctggccgtc atgtatggcc aggggatcat cccgcgcggg
      241 cccaaccccc tctatgcgta tcaggacgcc gaccggtcgg ccgccggcct gcgccagcta
      301 tcggagattc tggctcgcac ggtcgagggt ctgcccgacc acgaggcgta tctgagggcc
      361 aacgccatgt ggtctggcga cgcctagacg gcgggcgaag gctccagagg ctggaccggg
      421 atcgccggcg cagggacagc cggctcgggc gtctcgcgcc acgccgcggc ggggttcaag
      481 gccgccgtgg cttcgaggaa tgtccccggc ttcatcgacg gctcccgcgc cgtctgcagg
      541 ccggcgaccc gcgccagatc ggcgatatag ctgatgcagt tgcgccgcct caggtcgtag
      601 agcgaccctt ccggcccgtt ccaccagtcc gaacgcgcgc gcaccgcgcg atagacggcg
      661 tcgctgaccg ttatccgtag atacggcttc ccctccccca catagcgggc ctcgggcgtg
      721 gccacgattc ccggaccggt ggccagaagc agttgcggtc cgggatattt cgcggtgaag
      781 cccgccgccc agtcgacggc gtcgcccgtg tcgtccagcg ttccggtggc gtggacatag
      841 gcgtgcggga acagcagctc gccgccccgg atgcgcgcgc cgggatgggc gtagaaggtc
      901 agggtcacag ccgcccaggc gggggcggcg atcagggcca gaagcaggcc cagcagcccc
      961 gcgcgtctcc tcatggcgat cattcagctc tccgcggccc cgcgcctatc ctgcgagacc
     1021 cggacccaaa cgcgcaaggg ccgccgacga tgccgccggc ggccctgtct tttcgctcgc
     1081 gggatcagtg gccgccgggc gtcgctgcgg cggctgtgtt ccgggtcttt gcgcgcgagg
     1141 cggcgatggc gaagccgacc agcagcaggt agccgaccag cggcacatag aagaccgggt
     1201 tcagcgtcgc cgcattgtcc gcgatctggc ccgccaccgg cggcagcacc gcgccgccga
     1261 tgatgccgaa gaccagcagg cccgacgtcg ccggaaccgg cgcggtcgac cgctccagcg
     1321 tcagggtgaa gatggtcggg aacatgatcg agttgaagaa gccgatggcg atggcggcgt
     1381 aggcggcggt ggttccgctg gtctgggtga ccaccagaga cagggtggcc gccacgacgg
     1441 tgttgacgat caggatggtc gcagccggta tctttgtcag catcagcagg ccgccgacca
     1501 gacgcccaac catggccccg ccccagtagt acgcgaccat gctgccggcg gcggccagcg
     1561 ggatgttgag gatctgcggg ctgtgcagga agttggtcag cagatcgccg atggtcactt
     1621 ccgaaccgac gtagaagaag atggccaggg cgccgaacac ggcccacggc gatttcagcg
     1681 ccaccagcgg cgacatccgg tcgtccgcac tgggggcggg agcggcggcg ttgatcgtct
     1741 tgcgcgcgct gaagatgaag cccgccacca cggcgaagaa gacgcccacg gccaggaagg
     1801 ccaggtcgat gctgcgcaga gactcggcgc gcgacgccgg atcggtgatc accgcgtcgg
     1861 cggcgaagac gccccccgtc aacaggatcg gcgcggcgat cagcggcccc agcgtggtgc
     1921 ccagcgagtt gaagaactgc gagaagttga gcctggcgtg cgaactcttc tgcgccccca
     1981 gcgccgccac cagcggattg gcgccgacct gaagcagggt cacgcccgag gcgatcacga
     2041 acagggcgat gagcacgccg ggatagaagt cggccagggt ggcgatcggc acgatcaggc
     2101 agccagcgac catggtcacc aaggccacga tgatcgccct gctgtagccc agccggccga
     2161 ggatggcggc agccgggatg gatgcgatcc cgtaggcgat gaaccaggcg aaggtggtca
     2221 gggtcgcctc ggtatagctg agctcgaaca cccggcgcac ggcggctatc agcgggccga
     2281 tcagcgaggt
//
LOCUS       DASVVN010000593         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_154563,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000593 DASVVN010000000
VERSION     DASVVN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154563"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VF686_09680"
     CDS             complement(<1..727)
                     /locus_tag="VF686_09680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8662123.1"
                     /translation="MKQIDQMRRRAARYLDIDPVKIASPRGVFSLSFDDIPATAWTEA
                     GPVLAQHGVRATYYVCGGLAGGRNMDRDQFQVAHLQALHEAGHEVGCHTFSHTSVLRM
                     DAEALGLSLDANAAWVAERLDGHRMTTFAWPFGDATLGAKRVVRERFAMARGVRDGVN
                     AGREDRALIKSIGLESRRLPDYDLEALMAETATSKGWLNAYGHDVSDRPTDYGCTPDD
                     LDRVLRAAKAAGLEVLPVAAAWGV"
     gene            845..1732
                     /locus_tag="VF686_09685"
     CDS             845..1732
                     /locus_tag="VF686_09685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX8662124.1"
                     /translation="MTTEPLAFQDLILTLHKYWGDQGCAILQPYDIEVGAGTLHPATV
                     LRALGPRPWKAAYVQPSRRPGDGRYGENPNRLQHYYQYQVILKPNPENLQQLYLGSLA
                     AIGIDPTLHDIRFVEDDWENPTVGAWGLGWEVWCDGMEVTQFTYFQGVGGIEVDVVSG
                     ELTYGLERLAMYVQGVDNVYDLKFTKEGLTYGEVFLENERQQSEANFHGYDVDGLKRR
                     FEDMVGEVSNLLAMKGPQDQALVLPAYDQVLKASHLFNLMDARGAIAVAERQSYIGRI
                     RDLCKACATEWVEQERGRA"
     gene            1732..>2776
                     /gene="glyS"
                     /locus_tag="VF686_09690"
     CDS             1732..>2776
                     /gene="glyS"
                     /locus_tag="VF686_09690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX8662125.1"
                     /translation="MAQLLVELFSEEIPARMQQGAARDLERMASERLKAAGLTYEALT
                     TFAGPRRLTLVVEGLPAGTPDREEELKGPRTSAPEQALEGFLRKTGLTKDQLSERDGV
                     WFAIISEKGRPTAALISEMVDGVVRGFPWPKSMRWGSGTLRWIRPLKRIVALFDGAVV
                     PFEIDGIASGDITEGHRFLGSGQPFAVRDFADYRAKLEKQFVLLDVADRKLRILEGAQ
                     KACAARGLSLVDDDGLLDEVAGLAEWPTPILGDMDPQFLTLPPEVIRLSMKVHQKYFA
                     VRDPAAHGLAPHFVVVANVEATDGGKALAAGNSRVLSARLDDARFFWDEDLKTGFDAW
                     SKKLEGVTFHAKLG"
BASE COUNT          458 a          966 c          913 g          439 t
ORIGIN      
        1 ccacgcccca cgccgccgcg accggcagca cctccagccc cgcggccttg gccgcccgca
       61 gcacccggtc cagatcgtcc ggcgtgcagc catagtccgt cggccggtcc gagacgtcgt
      121 ggccataggc gttcagccaa cccttcgacg tcgccgtctc ggccatcagc gcctcgaggt
      181 cgtagtccgg caaccgccgg ctctccagcc cgatcgactt gatcagcgcc cggtcctcgc
      241 gtcccgcatt gaccccgtcg cgcaccccgc gggccatggc gaaccgttcg cgcaccaccc
      301 gtttagcgcc cagggtcgca tctccgaacg gccaggcgaa ggtcgtcatc cgatgcccgt
      361 ccagcctctc ggccacccag gcggcgttgg cgtccaggct caggcccagc gcctcggcgt
      421 ccatccgcag cacgctggtg tgactgaagg tgtggcagcc gacctcatgc cccgcttcat
      481 gcagcgcctg cagatgggcg acctgaaact ggtcgcggtc catgttgcgc ccgccggcca
      541 gcccgccgca gacatagtag gtcgccctga ccccatgctg ggccagcacc ggtcccgcct
      601 cggtccaggc ggtggcgggt atgtcgtcga agctcagcga gaagaccccg cgcggcgacg
      661 cgatcttcac cgggtcgatg tcgagatagc gcgcggcccg ccggcgcatc tggtcgatct
      721 gcttcatggc tacagttcgt acccgccgcc gcttaaggaa gcgtaggcgg ggtcatcgcc
      781 ttgcccggca aaggccgccc gtctagggtc cgcggccttc ttccccggac gaaaaagcct
      841 gcctgtgacc accgaaccgc tcgccttcca ggacctgatc ctcacgctcc acaaatactg
      901 gggggatcag ggctgcgcga tcctccagcc ctatgacatc gaggtcggcg ccggcaccct
      961 gcaccccgcg acggttctgc gcgccctcgg tccgcgccca tggaaggccg cctacgtcca
     1021 gccctcgcgc cgccccggcg atgggcgcta cggcgaaaat cccaatagat tacagcacta
     1081 ttaccagtat caagtgatcc tgaagccgaa ccccgagaac ctgcagcagc tttatctggg
     1141 ctcgctggcg gcgatcggga tcgatccgac cctgcacgac atccgcttcg tcgaggacga
     1201 ctgggagaac cccacggtcg gcgcctgggg tctgggatgg gaagtctggt gcgacggcat
     1261 ggaggtgacg cagttcacct atttccaggg cgtcggcggc atcgaggtgg acgtggtctc
     1321 gggcgagctg acctacgggc tggagcgtct ggccatgtac gttcagggcg tcgacaacgt
     1381 ctacgacctg aagttcacca aagagggcct gacctacggc gaggtcttcc ttgagaacga
     1441 gcgccagcag tcggaagcca acttccacgg ctatgacgtc gacgggctga agcgccggtt
     1501 cgaggacatg gtgggcgagg tctcgaacct gctggccatg aagggtccgc aggatcaggc
     1561 gctggtcctg ccggcctatg atcaggtgct gaaggccagc cacctgttca acctgatgga
     1621 cgcccgcggc gcgatcgccg tcgccgaacg ccagagctac atcggccgca tccgcgatct
     1681 ctgtaaggcc tgcgccaccg aatgggtcga gcaggaaagg gggcgcgcat aatggcccag
     1741 ctccttgtcg aactgttctc ggaagagatc cccgcccgca tgcagcaggg cgccgcgcgc
     1801 gacctcgaac gcatggcctc cgaacggctg aaggcggccg gtctgaccta tgaggcgctg
     1861 acgaccttcg ccggcccgcg ccggctgacg ttggtggtcg agggcctgcc ggccggaacc
     1921 ccggaccggg aagaggagct gaaaggcccc cgcacctcgg cgcccgaaca ggcgctggaa
     1981 ggcttcctgc gcaagacagg tctgacgaag gaccagctca gcgagcgcga cggcgtctgg
     2041 ttcgcaatca tcagcgaaaa gggccggccc accgccgccc tgatctccga aatggtcgac
     2101 ggcgtcgtcc gcggcttccc ctggcccaag tcgatgcgct ggggttcggg gacgctgcgc
     2161 tggatccggc cgctgaagcg gatcgtcgca ctgttcgacg gggcggtggt cccgttcgag
     2221 atcgacggca tcgcctcggg cgacatcact gagggccacc gcttcctcgg ctcaggccag
     2281 ccgtttgcgg tcagggattt cgccgattac cgcgccaagc tggagaagca gttcgtcctg
     2341 ctcgacgtcg ccgaccgcaa gctgcgcatc ctcgaggggg cgcagaaggc ctgcgcggcc
     2401 aggggtctgt cgctggtcga cgacgacggc ctgctggacg aggtggcggg gctggccgaa
     2461 tggccgacgc cgatcctggg cgacatggac ccgcagttcc tgaccctgcc gccggaggtc
     2521 atccggctgt cgatgaaggt gcaccagaag tatttcgccg tccgcgaccc cgcggcccac
     2581 gggctggccc cgcatttcgt cgtggtggcc aatgtcgagg cgactgacgg cggcaaggcg
     2641 ctggccgccg gcaacagccg cgtgctttcg gcccgtctgg atgacgcccg cttcttctgg
     2701 gacgaggacc tgaagaccgg cttcgacgcc tggtcgaaaa agctcgaagg cgtgaccttc
     2761 cacgcgaagc tgggga
//
LOCUS       DASVVN010000594         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_48696, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000594 DASVVN010000000
VERSION     DASVVN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48696"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1269)
                     /gene="murG"
                     /locus_tag="VF686_09695"
     CDS             complement(187..1269)
                     /gene="murG"
                     /locus_tag="VF686_09695"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX8662126.1"
                     /translation="MAKVCVVAAGGTGGHMFPAEALSRELAARGWRIVLATDHRGEAY
                     AHNFPAEERIALDAATGRGPVGLAKAGVAIARGVLKARQALNRLDAAVVVGFGGYPSA
                     PALIAALSQRRPTLLHEQNAVLGRTNRWLAPYVGAIASSFPDLQKVPQGLGARVTLIG
                     NPVRPDIRALYDRAWSPPAADGPIHVLVTGGSQGARILAETAPRAMAALPEAIRARLK
                     VQQQSRAETLEAAQQIYLEAGIAAEVAPFFRDMAQRLSAAHLVIGRAGASTCSELAVA
                     AVPSLLIPLKIAMDDHQRFNALSLAEAGGAVVLLEDDVTVQALTAALLPLLSDPARLS
                     AMSTGARSAALPDATRALADMVEAAG"
     gene            complement(1277..>2315)
                     /locus_tag="VF686_09700"
     CDS             complement(1277..>2315)
                     /locus_tag="VF686_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX8662127.1"
                     /translation="PAAILADQSISDPFHYSWRMIVWAGGGTVLMLLASLLSPRGVRR
                     IALLALLGAIVVMAALPFIGNEVKGAARWVNLGPFSLQPSEFAKPSLIVCAAWMFSEA
                     QKGQGVPGVSIAFGAWALTVGLLLIQPDIGQTLLITTTFMAVFFMAGVPLKWVAALAA
                     AGMAGVVGLYFTFSHMRDRLSRFLSPETTDTHQIDRASEAIRAGGLVGRGVGEGVMKR
                     SVPDLHTDFIYSVGAEEFGLVLSLAMIALYAFIVIRGMRRAMKLTDPFEQTAAAGLFM
                     LIGLQACINIAVNLNLIPTKGMTLPFISYGGSSMLAMGLTMGLALALTRRRPGAYEPG
                     ASLPQGRRILP"
BASE COUNT          385 a          821 c          814 g          295 t
ORIGIN      
        1 accagttggt tggcggccca tccgacgaag acgaaaccgg tcacgatcgc ggtgacgacg
       61 acccattccg gcagccagta gaactcgcgc cacaggcgga agatctcgcg cgtcaggtcg
      121 gtcacaaacg agggcatggc cccatataac ccggcgggcg ctcgacagcg accatctgcg
      181 cagggatcag ccggccgcct cgaccatatc ggccagcgcc cgcgtggcgt ccggcagggc
      241 ggcggagcgg gcgcccgtgc tcatggctga caggcgggcc ggatcggaca gcaggggcag
      301 aagcgccgcc gtcagcgcct gaacggtgac atcgtcttcc agcaacacca ccgccccgcc
      361 ggcttcggcc aggctgaggg cgttgaagcg ctggtggtcg tccatggcga tcttcagcgg
      421 gatcagcagc gagggcacgg cggccacggc cagttccgaa caggtcgagg cgccggcccg
      481 gccgatgacc agatgcgccg ccgacaggcg ttgcgccatg tcgcggaaga agggcgccac
      541 ctcggctgca atcccggcct cgaggtagat ctgctgcgcc gcctccagcg tctcggcccg
      601 tgactgctgc tgcaccttca gccgggcgcg gatggcctcg ggcagggccg ccattgcccg
      661 gggcgcggtc tcggcgagga tgcgggcgcc ctgactgccg ccggtgacca gcacatggat
      721 cggcccgtcc gccgcgggcg ggctccatgc gcggtcgtag agggcgcgga tgtcgggacg
      781 caccggattg ccgatgagcg tcactcgcgc gccgagcccc tgcgggacct tctgcaagtc
      841 cggaaacgac gaggcgatgg cccccacata gggcgccagc cagcggttgg tgcgccccag
      901 cacggcgttc tgttcgtgca gcagcgtcgg ccgccgctgg ctcaacgccg cgatcagggc
      961 gggcgccgac ggatagccgc cgaaacccac caccaccgcc gcgtccagcc ggttcagcgc
     1021 ctggcgggcc ttcagaacgc cccgcgcgat ggcgacgccc gccttcgcca agccgaccgg
     1081 cccgcgcccc gtggccgcgt ccagcgcgat ccgctcctcg gcggggaagt tgtgggcata
     1141 ggcctcgccg cgatggtccg tggccagcac gatgcgccag ccccgcgccg ccagctcgcg
     1201 cgacagggcc tcggccggaa acatatggcc cccggtgccc ccggcggcga cgacgcagac
     1261 tttggccatg ggacccctag ggcaatatac gccggccttg cggcaggctg gccccgggct
     1321 cgtaggcgcc ggggcggcgg cgggtcaagg ccagggccag tcccatggtc aggcccatgg
     1381 ccagcatcga cgagccgccg tagctgatga atggcagggt catacccttg gtcgggatca
     1441 ggttcaggtt caccgcgatg ttgatgcagg cctgcagccc gatcagcatg aacagccccg
     1501 ccgcggccgt ctgctcgaat ggatcggtca gcttcatcgc ccgccgcatg ccgcgaatga
     1561 cgatgaaggc gtagagggcg atcatggcca gcgacagcac caggccgaac tcctcggccc
     1621 cgacggaata gatgaagtcg gtgtgcaagt cgggcaccga ccgcttcatc accccctcgc
     1681 ccacgccgcg cccgaccagt ccgccggcgc ggatcgcctc ggacgcacga tcgatctggt
     1741 gggtgtccgt ggtctcgggc gacaggaagc ggctgagccg gtcgcgcatg tggctgaagg
     1801 tgaaatacag gcccaccacg ccggccatgc ccgccgccgc gagcgcggcc acccatttca
     1861 gcggcacgcc ggccataaag aagacggcca tgaaggtggt ggtgatcagc agggtctggc
     1921 cgatgtcggg ctggatcagc agcaggccca cggtcagggc ccaggcgccg aaggcgatgc
     1981 tgacgcccgg caccccctgc cctttctggg cctcggagaa catccaggcg gcgcagacga
     2041 tcaggctggg cttggcgaac tcggacggct gcaggctgaa ggggccgagg ttcacccagc
     2101 gggccgcgcc cttcacctca ttgccgatga acggcagggc cgccatgacg acgatggcgc
     2161 ccagcagggc cagcagggcg atccgccgca cgccccgcgg cgacagcagg gaggccagca
     2221 gcatcagcac cgtgccgccc ccggcccaga cgatcatccg ccacgaatag tggaacgggt
     2281 cgctgatcga ctggtccgcc agaatggccg cgggg
//
LOCUS       DASVVN010000595         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_288401,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000595 DASVVN010000000
VERSION     DASVVN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288401"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VF686_09705"
     CDS             complement(<1..827)
                     /locus_tag="VF686_09705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8662128.1"
                     /translation="MASSPDVIVIGAGVLGLCTAAELGARGHAVTVVDPGGPNASAVA
                     AGMLAPALESLLDDLSPDRAALLKRARDLWPAVAEAHGLKLHRDGADWRGPDAAEALD
                     RLRRLGFDARPAKGGLTTPEDWRIDVGSTLTALARAPGVSVVRGRVMRLAGEGRRWRV
                     EAGDGRVWFSPTVVLATGADPAIMGLPDPVAAVVTGVQPIRGQLTAVATAAPARVIRA
                     SNVYVAPGEGGVVIGATMEPGRRDLEPDAAESARQVEAGLALLGAAGEAGAVRVGIRG
                     "
     gene            complement(910..>2284)
                     /locus_tag="VF686_09710"
     CDS             complement(910..>2284)
                     /locus_tag="VF686_09710"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX8662129.1"
                     /translation="MTRMGQAALDAIGENGFFVPALHTVGKPLAPGETDVPWPCNDDK
                     WIVHYPETREIWSYGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILKLTDPKG
                     RAKYVAAAFPSACGKTNLAMLQPTIPGWKAETIGDDIAWMRFGDDGRLYAVNPEAGFF
                     GVAPGTSRNTNGNALETLATNTVFTNTALTADGDVWWEGLTKEVPEGLTTWKGVPHDP
                     ASGEPAAHPNARFAAPASQCPTIAPEWEDPRGVPIDAILFGGRRASAVPLVTEAFDWE
                     HGVFMGSSVASEGTAAAENAIGTLRRDPFAMLPFCGYNMGDYFAHWLSMGGRTDAAKL
                     PRLYFVNWFRKNEDGKFVWPGFGDNARVLKWISERLDGEAEAVDTPVGRVPTREALDL
                     SGLSLSDADLSTLLDVDAEVWAEEASLIPDFYAQFGDRLPARLWAQHEALTARIDANR
                     AAAIAAE"
BASE COUNT          327 a          771 c          861 g          325 t
ORIGIN      
        1 ccgcggatcc cgacgcgaac ggcgcccgcc tcgccggcgg cgcccaacag ggccagaccg
       61 gcctcgacct gacgcgcgga ttcggcggcg tcgggctcca gatcacggcg gccgggttcc
      121 atagtcgcgc cgatgacgac accgccctct cccggagcga catagacgtt ggaggcccgg
      181 attacgcggg ccggggccgc ggtggcgacg gcggtcagct ggccgcggat gggctggacg
      241 ccggtcacga cagcagcgac cggatcggga aggcccatga tcgccgggtc cgcgccggtg
      301 gccagaacca ccgtcggact gaaccagacc cgcccgtcgc ccgcctcgac ccgccagcgt
      361 cgcccttcgc ccgccagacg catgacccgg ccccgcacca ccgagacgcc gggcgcgcgg
      421 gccagggcgg tcagcgtcga accgacgtcg atgcgccagt cttcgggggt ggtcaggccg
      481 cctttcgcag ggcgggcgtc gaagccgagg cgacgcagac ggtcgagggc ctcagcggcg
      541 tcgggaccgc gccagtcggc gccgtcccga tgaagcttca ggccatgcgc ctccgcgacg
      601 gccggccaga ggtcccgcgc ccgcttcagc agggcggcgc ggtcgggcga caggtcgtcg
      661 agcagggatt ccagggcggg ggccagcatg ccggccgcga cggcgctggc gttcggaccg
      721 cccggatcga cgacggtcac ggcatggccg cgcgcgccca gctccgcagc ggtgcagagg
      781 ccgagcacgc cggcgccgat gacgattacg tctggagagg aagccacggg cgatctgatc
      841 gaccccccgg ggcttctttg caaggggcgc tatcgcgagc cgcgcggcgg ctcgctgctt
      901 gagcgccccc tattcggcgg cgatggcggc ggcgcggttc gcgtcgatgc gggcggtcag
      961 ggcttcatgc tgggcccaca ggcgagcggg cagacgatct ccgaactggg cgtagaagtc
     1021 cgggatcagc gacgcttcct cggcccagac ctcggcgtcc acgtccagca gggtggacag
     1081 gtcggcgtcc gacaggctca ggcccgacag gtccagcgct tcgcgggtgg ggacgcggcc
     1141 cacgggcgtg tcgacggcct cggcctcgcc gtcgaggcgc tccgagatcc atttgaggac
     1201 ccgggcgttg tcgccaaagc ccggccagac gaacttgccg tcctcgttct tgcggaacca
     1261 gttgacgaag tagaggcgcg gcaacttggc ggcgtccgtg cgtccgccca tcgacagcca
     1321 gtgagcaaag tagtcgccca tgttgtagcc gcagaagggc agcatggcga agggatcgcg
     1381 gcgcagggtc ccgatggcgt tctcggcggc ggcggtgcct tccgaggcga ccgacgagcc
     1441 catgaagacg ccgtgttccc agtcgaaggc ctccgtcacc agtggcacgg ccgaggcgcg
     1501 gcggccgccg aacaggatgg cgtcgatcgg cacgccgcgg gggtcttccc attccggcgc
     1561 gatggtgggg cactgcgagg ccggggcggc gaagcgggcg ttcgggtgag ccgcgggctc
     1621 gcccgaagcc gggtcgtggg gcacgccctt ccacgtggtc agaccctctg gcacttcctt
     1681 cgtcaggcct tcccaccaga cgtcgccgtc ggcggtcagg gcggtgttgg tgaagacggt
     1741 gttggtggcc agggtctcga gcgcattgcc gttggtgttg cggctggtgc caggggcgac
     1801 gccgaagaag ccggcttccg gattgacggc gtacaggcga ccgtcgtcgc cgaagcgcat
     1861 ccaggcgatg tcgtcgccga tggtctcggc cttccagccc ggaatggtcg gctggagcat
     1921 ggccagattg gtcttgccgc aggccgaggg gaaggcggcg gcgacgtatt tggcgcggcc
     1981 cttcggatcg gtcagcttga ggatcagcat gtgttcggcc agccagccct cgtcgcgggc
     2041 catgaccgag gcgatgcgca gggcgtagca tttcttgccc agcagggcgt tgccgccgta
     2101 gcccgagccg taggaccaga tttcgcgggt ctcgggatag tggacgatcc atttgtcgtc
     2161 gttgcagggc cacggcacgt ccgtctcgcc gggcgccagc ggcttgccga cggtgtgcag
     2221 ggccggtacg aagaagccgt tctcgccgat cgcgtccagg gccgcctggc ccatgcgggt
     2281 catg
//
LOCUS       DASVVN010000596         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_154985,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000596 DASVVN010000000
VERSION     DASVVN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154985"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..742)
                     /locus_tag="VF686_09715"
     CDS             complement(242..742)
                     /locus_tag="VF686_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8662130.1"
                     /translation="MSYLARDDRPADKAARYAELEEEILAVLDGEPNRTALMATVASM
                     LADAFPVFFWTGFYVVDPDKADELVVGPYQGTLGCLRIAFGRGVCGTAARERKTQIVD
                     DVHAFPGHIACDARSASEIVVPVFDPSGALIAVFDVDATETSAFDASDAYALERLMAK
                     VFGSTE"
     gene            1010..2488
                     /locus_tag="VF686_09720"
     CDS             1010..2488
                     /locus_tag="VF686_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8662131.1"
                     /translation="MRDDRSDHGDQPRNGRRGPIAIAAVAGVLALAAIGMVSCGDRDG
                     VRFWQPTGGAAGNACPRPASLTGSEFNAQETGLRALRRAAFRGDFFAQLELGARYAAV
                     RATDKNIEDPIESATWYALALANDEGYAPINRVDRGGFGGWRPLSKYDDCRAYERHSA
                     YQRLDRQLGQMTREEQEAVRDRVIYVLSTQDADGYRTLARLHDGLYGPFGEPEDNREA
                     REAWGRGKTGSGRDRSRRGYYSATNLFQRNDVDAYLYNYLAVQTGDVGAYVLLKDFER
                     SAPSRDQYQRFVETKARQWTPPFEFYPNSAPQSGVPFSDESRPRGDAYEYALGRMASE
                     LPFVHVGRALRYLGITDEVAGRPEDLSKRQIETLEAMLGHPMEGRLSYLERVRAIQMA
                     AINGSSEAQLVLAVMYAEGIGLPADYARAFKWFDEAARQGSPEAKFAMSSYFALGVSG
                     VADQDKAQAVALRIDSALSGFGPSAGRLQAVLAQVSRSQQRR"
BASE COUNT          423 a          859 c          872 g          394 t
ORIGIN      
        1 cagctcgagg accccctcgg gcgcggtcag gccggatgcg tggtcgatga tcgccacctt
       61 cagcccgtca gccagcgccg cctcgacgac agcccggccg agcacgccat ggccgcccgt
      121 aaccgcgatc acccgttccg acatgccgca cctcccaagc caattcgggc accctttgca
      181 cgggtcccga acggaaaaac acagtctaca gcgtctacag cgtctacagc gtctacagcg
      241 tctactcggt cgacccgaag accttcgcca tcagccgttc cagagcatag gcgtccgatg
      301 cgtcaaaagc ggacgtctcg gtcgcgtcga cgtcgaagac ggcgatcagg gcgccggatg
      361 ggtcgaacac cggcaccacg atctcgctgg cggagcgggc gtcgcaggcg atgtggccgg
      421 ggaaggcgtg gacgtcatcg acgatctgcg tctttcgctc gcgggcggcc gtaccgcaga
      481 cgccgcggcc gaaggcgatg cgcaggcatc ccagcgtgcc ctgatagggc ccgaccacca
      541 gttcgtcggc cttgtccgga tccaccacgt agaagccggt ccagaagaag acggggaagg
      601 cgtcggccaa catggaggcg acggtggcca tgagcgcggt gcggttgggc tcgccgtcca
      661 gcacggccag gatctcttcc tcgagctcgg cgtagcgcgc ggccttgtcg gcgggacggt
      721 cgtcgcgggc gaggtaggac atggaggctc cgggcggtct ggtccgcagc atatagggag
      781 gcccgccgtc actggcgaga ggcgtgtttt cccctttcag gcgagtgcat aaccagctga
      841 ttcgtgggga taagtacgga cgcggacaca aaagggccgc cctgcggcaa cgctttgtcg
      901 caaaccagcg ttaaccttga cagatggtta actggacgga actgtcacgc ttgcgcaagg
      961 tgagcggacg ggccccgggg ctgctggcta tttgatcgaa ggggggcgta tgcgcgacga
     1021 tcgatccgac catggcgacc agccccgcaa cggacgacgc ggccccatcg ccatcgcggc
     1081 ggtggcgggc gttctggcgc tggccgccat cggcatggtc tcgtgcggcg atcgcgacgg
     1141 cgtgcgcttc tggcaaccca caggcggcgc ggccggcaac gcctgtcccc gcccggcgtc
     1201 cctgaccggt tccgaattca acgcccagga gacgggcctg cgggccctgc gccgcgcggc
     1261 cttccgcggc gacttcttcg cccagctgga actgggcgcc cgctacgccg ccgtgcgcgc
     1321 cacagacaag aacatagagg atccgatcga gagcgcgacc tggtacgcgc tcgcccttgc
     1381 gaacgacgag ggctacgccc cgatcaaccg cgtcgaccgg ggcggttttg gcggctggcg
     1441 gccgctgtcg aaatacgacg actgccgggc gtatgagcgg cactccgcct atcagcggct
     1501 ggaccgtcag ctgggccaga tgacgcgcga ggagcaggag gccgtccgcg accgcgtcat
     1561 ctatgtgctg tcgacccagg acgccgacgg ctatcgcacc ctggcgcggc tgcacgacgg
     1621 cctgtacggc ccgttcggcg aacccgagga caatcgcgag gcgcgcgagg cctggggtcg
     1681 tggcaagaca ggctccggtc gcgaccgcag ccgccggggc tattactcgg ccaccaatct
     1741 gttccagcgc aacgacgtcg acgcctatct gtacaactac cttgcagtgc agaccggcga
     1801 cgtcggggcc tatgtcctgc tgaaggattt cgagcgctcg gcccccagcc gcgaccagta
     1861 ccagcggttc gtcgagacca aggcgaggca gtggacgccg ccgttcgaat tctatcccaa
     1921 cagcgcgccg cagtcggggg tgcccttctc ggacgagagc cgtccgcgcg gcgacgccta
     1981 tgagtatgcg ctgggccgga tggcgagcga actgcccttc gtccatgtcg gccgggcgtt
     2041 gcgttacctc gggatcactg acgaggtcgc cggccgtccc gaggatttgt ccaaacgcca
     2101 gatcgagacg ctggaggcca tgctgggcca tccgatggag ggtcggctgt cgtatctgga
     2161 gcgggttcgc gcgatccaga tggccgccat caacggatcg tccgaggcgc agctggtgct
     2221 ggcggtgatg tacgccgagg gcatcggcct gcccgccgac tacgcccgcg ccttcaaatg
     2281 gttcgacgag gcggcccgtc agggcagccc cgaggccaag ttcgccatgt cgagctattt
     2341 cgcgctcggc gtctcgggcg tggccgatca ggacaaggcc caggccgtgg ccctgaggat
     2401 cgattccgcc ctgtcgggct tcgggccctc ggccggccgc ctgcaggccg ttctggccca
     2461 ggtctcccgt tcgcagcagc gccgctaagg agggcgtgag aatcatgacg agctccagac
     2521 ccttcgcccg gatcgccagc ctgatcgg
//
LOCUS       DASVVN010000597         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_259170,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000597 DASVVN010000000
VERSION     DASVVN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259170"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1620
                     /gene="murC"
                     /locus_tag="VF686_09725"
     CDS             190..1620
                     /gene="murC"
                     /locus_tag="VF686_09725"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX8662132.1"
                     /translation="MIARLRPVPFDLGPVHFVGIGGIGMSGIAEIMLKIGYSVQGSDA
                     RASANTERLERLGARIFIGHDAAHIGDGVSAVVYSTAVKPTNPEMVAARERRIPLVRR
                     AEMLAELMRLQFSIAVGGTHGKTTTTSMVAAILDAGGLDPTVVNGGIINAYGTNAKVG
                     DGDWIVVEADESDGSFLRLKSTVAIVTNIDPEHLDHYGDFDGVRKAFVDFVENIPFYG
                     FAAVCLDHPEVQRLVASIDNRRLVTYGINPQAMVRADNCQMMPDGCRFDVVVQGQGLA
                     ALEEPIRIEGLHLPMAGWHNVANALAAIAVARELDVSDEAIKAGLAGFGGVRRRFTTT
                     GVVGGVRIVDDYGHHPVEIAAVLKAARQVAEGRVIAVVQPHRFTRLESLMEEFSTCFS
                     DADVVYVADVYAAGEVPIEGVDKHALVDGVRRFGHRSVQPLDSVEALPGVIAAEAKPG
                     DLVVLLGAGDITAWAYALPGQLEALG"
     gene            1617..2519
                     /gene="murB"
                     /locus_tag="VF686_09730"
     CDS             1617..2519
                     /gene="murB"
                     /locus_tag="VF686_09730"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8662133.1"
                     /translation="MIALPEVRGKLLRDEPLGPFTWFRVGGNADVLFIPADGDDLAEF
                     LKALDPAVPVTVLGVGSNVIVRDGGVEGVVVRLAGRPFAGITTEGDTITAGTGALDSM
                     VAKASAKAGLAGLEFYAGIPGTIGGALTMNAGCYGSETKDILVSARGVTRSGERVEYA
                     LADFGYTYRHSEAPADIIWLEATYRGTPADPEAVSAKIAEITSRREQTQPIREKTGGS
                     TFKNPPGHSSWKLVDEAGWRGKLFRATGGGAMFSELHSNFMINPGEATAADIEGLGNA
                     VRADVLSKRNVNLEWEIKRIGRPL"
     gene            2531..3466
                     /locus_tag="VF686_09735"
     CDS             2531..3466
                     /locus_tag="VF686_09735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8662134.1"
                     /translation="MTRPFADLHVAVLMGGLSSEREVSLSSGGMCADALEGQVAKVTR
                     VDAGRDLAQVLAALKPDVVFNALHGEWGEDGCVQGVLETLAIPYTHSGVLASALAMDK
                     DKSKAVLRAAGVVVPGGGLFDRHEVARRHVIDPPYVIKPNAEGSSVGVYLVREGDEPC
                     AATADRSWTYGEQVMVEPFIAGKELAVTVLGEKTGPRALTVTDITPHKGFYDYEAKYA
                     PGGSSHILPADLPPHIFERALRESELAHAAMGCRGVSRSDFRYDDIKDDLVLLEVNTQ
                     PGMTPTSLSPEQAAFVGMDYKALVRWMVEDASCPR"
     gene            3454..4341
                     /locus_tag="VF686_09740"
     CDS             3454..4341
                     /locus_tag="VF686_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX8662135.1"
                     /translation="MPAVVRGGRRQSSNQPAKRGAAPGSQGRGRSPRNAPATPGKMAA
                     LGRLDLSPRAVIISIVAGVAVLAGVLATGARAERIGASISHGVDGATARMGLKLEKVF
                     IEGESPEATATIQRAVQLYADQPMTSIDLDAVRKRVEAVGWVKGARVVRLLPNTLIVH
                     VTEHDRLAVWQAGGRNMVIDSHGAVIAGADAGRYPNLPLVVGKGAEQAASEVLPLIAQ
                     RPGLQARVEALVRVDERRWDLRLKDGSLIQLPATDQDAALIQLDALDQRERLLELGFD
                     RIDLRTPGEVAVRPSAGAA"
BASE COUNT          689 a         1470 c         1540 g          702 t
ORIGIN      
        1 ctacgaccaa ttctgacgca ggcgcgtggc atggttcgcg cggatggttc aggggaggtg
       61 gaggtgtcca gagtccgatg tgtccgaatt gtccgccata aattgaccgg actcgaccac
      121 tttgagcgcc gggcgaggga actgcggatc ggtggtgcaa tccgtccgaa gccttgctaa
      181 tccgctggga tgattgctcg ccttcgcccc gtaccgttcg acctcggccc cgtccatttc
      241 gtgggcatag gcgggatcgg gatgagcggg atcgccgaaa tcatgctcaa gatcggctat
      301 tcggttcagg gttccgacgc cagggccagc gccaacaccg agcggctgga acggttgggc
      361 gcgcgcatct tcatcggcca tgacgccgcc cacatcggcg acggcgtctc ggcggtggtc
      421 tattcgacgg cggtcaagcc caccaacccc gagatggttg cggcccgcga gcgccgcatc
      481 ccgctggtgc gccgggccga gatgctggcc gagctgatgc ggctgcagtt ctcgatcgcg
      541 gtcggcggca cccacggcaa gaccacgacc acctccatgg tcgcggcgat cctcgatgcg
      601 ggcgggctgg atccgaccgt ggtcaatggc gggatcatca acgcctatgg caccaacgcc
      661 aaggtcgggg acggcgactg gatcgtggtc gaggccgacg agagcgacgg cagcttcctg
      721 cgcctcaagt cgacggtggc catcgtcacc aacatcgacc ccgagcatct ggatcactac
      781 ggcgacttcg acggggtgcg gaaggcgttc gtggacttcg tcgagaacat ccccttctac
      841 ggcttcgccg ccgtctgcct cgaccatccc gaggtgcagc ggctggtggc ctcgatcgat
      901 aaccggcggc tggtgaccta cggcatcaac ccccaggcga tggtccgggc cgacaactgc
      961 cagatgatgc ccgatggctg ccgcttcgat gtggtcgttc agggccaggg tctggccgcg
     1021 cttgaagagc cgatccggat cgagggcctg cacctgccca tggccggctg gcacaatgtc
     1081 gccaacgccc tggccgccat cgccgtggcc cgcgaactgg acgtgtcgga tgaggccatc
     1141 aaggcaggtc tggccggctt cggcggggtg cgccggcgtt tcaccaccac gggcgtggtc
     1201 ggcggggtgc ggatcgtgga cgactacggc caccacccgg tcgagatcgc cgccgtgctc
     1261 aaggccgcgc gtcaggtcgc cgagggccgc gtcatcgccg tggtccagcc gcaccgcttc
     1321 acccggctcg aatccctgat ggaggagttc tcgacctgct tctccgacgc cgacgtcgtc
     1381 tatgtcgccg acgtctatgc tgcgggcgag gttcccatcg agggcgtcga caaacacgcc
     1441 ctggtcgatg gcgtccgccg cttcggccac cgctcggtcc agccgctgga cagcgtcgag
     1501 gccttgccgg gcgtgatcgc ggccgaggcg aaaccgggcg acctcgtcgt cctgctcggc
     1561 gccggcgaca tcaccgcctg ggcctacgcc ctgccgggcc agctggaggc gctgggttga
     1621 tcgccctgcc tgaggttcgc ggcaagctgc tgcgcgacga gccgttgggg ccgttcacct
     1681 ggtttcgggt gggcgggaac gccgacgttc tgttcatccc cgccgacggc gatgatctgg
     1741 ccgaattcct caaggcgctg gaccctgctg tgccggtgac ggtgctgggc gtgggctcca
     1801 acgtcatcgt ccgcgacgga ggggtggagg gcgtggtcgt gcggctggcc gggcggccct
     1861 tcgccgggat cacgaccgaa ggcgacacca tcaccgccgg cacgggcgcg ctggattcca
     1921 tggtggcgaa ggcgtccgcg aaggcggggt tggccgggct ggagttctac gccggcatcc
     1981 ccggcaccat cggcggcgcc ctgaccatga acgccggctg ctatggctcg gagaccaagg
     2041 acatcctcgt ttcggcccgc ggcgtgacgc ggtcgggcga gcgggtcgaa tatgcgctgg
     2101 ccgacttcgg ctacacctac cgccactccg aggcgccggc cgacatcatc tggctggagg
     2161 cgacgtatcg cgggacgccc gccgatccgg aggccgtgtc ggcgaagatc gccgagatca
     2221 cctcgcgccg cgaacagacg cagccgatcc gcgagaagac cggcggctcg acgttcaaga
     2281 acccgcccgg ccattcgtca tggaagctgg tcgacgaggc gggctggcgc ggcaagttgt
     2341 tcagggccac cggcggcggg gcgatgttca gcgaattgca cagcaatttc atgatcaacc
     2401 cgggcgaggc gaccgccgcc gacatcgagg gtctgggcaa cgccgtgcgc gccgacgttc
     2461 taagtaaacg gaacgtaaac ctcgaatggg agatcaagag gatcggccgc ccgctttgag
     2521 ttgatccccc atgacccgcc cgttcgcaga tctgcacgtc gccgtcctga tgggcgggct
     2581 gtcgtccgaa cgagaggtgt cgctgagctc gggcggcatg tgcgccgacg ccctggaagg
     2641 gcaggtggcc aaggtcaccc gcgtcgacgc cggccgcgac ctcgcgcagg tgctggcggc
     2701 gctgaaaccc gacgtcgtct tcaacgccct gcacggcgaa tggggcgagg acggctgcgt
     2761 ccagggcgtg ctggagacgc tggccattcc ctatacccac tcgggcgtgc tggcctcggc
     2821 gctggccatg gacaaggaca agtccaaggc cgtgttgcgc gccgccggcg tcgtggtgcc
     2881 cggaggcggc ctgttcgatc gacatgaggt ggcgcgccgc cacgtgatcg atccgcccta
     2941 tgtcatcaag cccaacgccg agggctcgtc ggtcggcgtc tatctggtgc gcgagggcga
     3001 cgaaccttgc gccgccaccg cagaccggtc gtggacctat ggcgaacagg tgatggtcga
     3061 gcccttcatc gccggcaagg aactggcggt cacggttctc ggggagaaga ccggcccgcg
     3121 cgccctgacg gtcaccgaca tcacgccgca caagggtttc tacgactacg aggccaaata
     3181 cgcgccgggc ggctccagcc acatcctgcc ggcggatctg ccgccgcaca ttttcgagag
     3241 agcgctgcgg gaatccgaac tcgcgcacgc cgccatgggg tgccgcggcg tgtcgcgatc
     3301 ggactttcgt tatgacgata ttaaggacga tctggtcctt ctggaggtca atacccagcc
     3361 cggtatgacg ccgacctcgc ttagtcccga gcaggccgcg ttcgtcggga tggactataa
     3421 agcattggtg cggtggatgg tggaggacgc ctcatgcccg cggtagttcg cggcggtcgg
     3481 cgacagagtt cgaaccagcc tgcaaaacgc ggcgcggctc ccggatcaca gggacgcggg
     3541 cgttcgcccc gcaacgctcc ggccacgccg ggcaagatgg cggcgctggg ccgtctcgac
     3601 ctgtctcccc gcgcggtcat catctccatc gtcgccggcg tcgccgtcct ggcgggcgtg
     3661 ctggcgaccg gcgcgcgggc agagcgtatt ggcgcctcga tcagccacgg cgtcgatggc
     3721 gccacggcgc gcatgggcct caaactcgag aaggtgttca tcgagggcga gtcgcccgag
     3781 gcgaccgcca ccatccagcg cgccgtgcag ctctacgccg accagccgat gacctcgatc
     3841 gacctggacg ccgtgcgcaa gcgcgtcgag gccgtgggct gggtcaaggg cgcgcgtgtg
     3901 gtgcgcctgc tgcccaacac cctgatcgtc cacgtgaccg agcatgatcg tctggccgtc
     3961 tggcaggccg gcggccgcaa catggtcatc gacagccatg gcgccgtgat cgccggcgcc
     4021 gacgccggcc gctatcccaa tctgccgctg gtcgtcggca agggcgccga acaggccgcg
     4081 tccgaggtcc tgcccctgat cgcgcagcgg cccggcctgc aggcgcgggt cgaggccctt
     4141 gtgcgcgtcg acgaacgtcg ctgggacctg agactgaagg acggcagcct gatccagctg
     4201 cccgccaccg accaggacgc cgccctgatc cagctggatg cgctcgacca gcgcgaacgg
     4261 ctgctggagc tgggcttcga ccgcattgat ctgagaacgc cgggggaggt cgccgtccgg
     4321 ccctccgccg gcgccgcgta gggtagggga cgcttatggc cggacggtct gtggacaaaa
     4381 ctgtgggcgg cgccaagggc g
//
LOCUS       DASVVN010000598         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_469917,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000598 DASVVN010000000
VERSION     DASVVN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469917"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VF686_09745"
     CDS             <1..926
                     /locus_tag="VF686_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8662136.1"
                     /translation="SNAMKFTDTGSITLRLKAWADEPGGHAVLIEVADTGPGMTADQL
                     ARLFTPFDQTIAGVSAVHGGTGLGLAISRQLVQLMDGRLTARSEPGSGARFTLSLHLE
                     PGEAVAVQTPALDQQSRDAVARSLSGHRSAAAEQIAAPQPLYEAPPVPAQLVVEDAAP
                     EAEYEDEDEGRPMRVLVVDDHDINRRAIQLILQPLGCDIATAADGMQALKICEHTAFD
                     LIFMDVRMPELDGRETTRRIRAGDGPNVLVPIIAVTADTSADDIAACTAAGMTYFVPK
                     PITPPMLLGAITHVMTMAQSAEVAEAETVAA"
     gene            complement(884..1333)
                     /locus_tag="VF686_09750"
     CDS             complement(884..1333)
                     /locus_tag="VF686_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX8662137.1"
                     /translation="MGTDVFTPEQRSAVMRRVKSRDTTPELAVRRILRAAGIGYRLGG
                     AGLPGRPDLVMKGRRVAVFVHGCFWHGHDCARGSRKPKANAAYWTAKIDRNRARDEAS
                     RTALNAAGWRVITVWECAMKAEGFADRLIADVRDQAATVSASATSAD"
     gene            complement(1470..1955)
                     /locus_tag="VF686_09755"
     CDS             complement(1470..1955)
                     /locus_tag="VF686_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8662138.1"
                     /translation="MTVQIGDRIPSANLTRATDDGPRPVTTDEVFAGKTVALFAVPGA
                     FTPTCSAKHLPGFKDLREDIVGKGVDIIACVSVNDAFVMGAWADSQGIGKDEIVMLGD
                     GNGDFTRQLGLSMDAKGFGMGERSQRYSMLVRDGVVEQLNIEQPGEFKVSSAEHLLAQ
                     L"
     gene            2081..2650
                     /locus_tag="VF686_09760"
     CDS             2081..2650
                     /locus_tag="VF686_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX8662139.1"
                     /translation="MDEFQSLAGRLLVAMPGISDPRFEHAVILICAHRPDHAMGLRLD
                     RPAPGVNLKKVLTKLDTPLPPDADSRAVLIGGPVDRERGFVLHTDDWLVDDASLAFGA
                     GFAMTGTREALLAMVDPETGPHRSVLLLGYAGWGDGQLEDELGENVWLVAEADPELIF
                     DTDHESKWGRVLAGLGVDPAQLSGQIGRA"
     gene            complement(2659..>3198)
                     /locus_tag="VF686_09765"
     CDS             complement(2659..>3198)
                     /locus_tag="VF686_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8662140.1"
                     /translation="KLFCSVNLSVGEIERPDLVADVTRIITESGLPKGALKLEVTEGD
                     IMRDTSRAAETLTRLREAGASLALDDFGTGFSSLTWLAKLPFDTLKIDRYFVLTMDKD
                     EGSAKIVKSVVNLGRDLGLDVVAEGVENAGLAKLLLEAQCHYGQGFGYAQALPAQEAE
                     VYLNESLADGTAPIKARSA"
BASE COUNT          499 a         1122 c         1084 g          493 t
ORIGIN      
        1 tctccaatgc gatgaaattc accgacaccg gatcgatcac cctgcgcctc aaggcctggg
       61 ccgacgagcc gggcggccac gcggtgctga tcgaggtggc cgacaccggt ccgggcatga
      121 ccgccgacca gctggcccgc ctgttcaccc cgttcgacca gaccatcgct ggcgtcagcg
      181 ccgtccacgg cggcacgggt ctgggcttgg ccatcagccg tcagctggtc cagctgatgg
      241 acggccggct gaccgcgcgg agcgagcccg gatcgggcgc ccggttcacc ctgtcgctgc
      301 atctggagcc cggcgaggcc gtcgccgtgc agactcccgc cttggatcag cagagccgag
      361 acgccgtcgc ccgttcgctg tccggccatc gctcggctgc cgccgaacag atcgccgcgc
      421 cccagccgct gtacgaggcc ccgccggttc ccgcccagct ggtggtcgag gacgccgctc
      481 ccgaagcgga atacgaggac gaggacgaag gccgtcccat gcgggtgctg gtcgtcgacg
      541 accacgacat caaccgccgc gccatccagc tgatcctgca gccgctgggc tgcgacatcg
      601 ccacggccgc ggacggcatg caggcgctga agatctgcga gcacaccgcc ttcgacctga
      661 tcttcatgga cgtgcggatg ccggaactgg acgggcgcga gaccacccgc cgcatccgcg
      721 ccggcgacgg ccccaacgtc ctcgtgccga tcatcgcggt gaccgccgac acctcggccg
      781 acgatatcgc cgcctgtacg gccgccggca tgacctattt cgtgcccaag ccgatcactc
      841 cgccgatgct gctgggcgcc atcacccatg tgatgaccat ggctcagtcg gcagaggtcg
      901 ccgaggcgga gaccgtcgcg gcctgatccc gcacgtcggc gatcagccgg tcggcgaaac
      961 cctccgcctt catggcgcac tcccagactg tgatcacgcg ccagccggcg gcgttcagcg
     1021 cggtgcggct ggcctcgtct cgggcccggt tgcggtcgat cttggcggtc cagtaggcgg
     1081 cgttggcctt gggcttgcgc gagcctcgcg cgcagtcgtg gccatgccag aaacagccgt
     1141 ggacgaagac cgcgaccctg cgccccttca tcaccagatc gggccgcccc ggcaggcccg
     1201 cgccgcccag ccggtagccg atgccggccg cgcgcaatat gcggcgcacg gccagctcag
     1261 gcgtcgtgtc acggctcttc acccggcgca tgactgcgct gcgctgctcg ggggtgaaga
     1321 cgtcggttcc catcaccatg catcccggcg aatgccggga tccagatggc tgaagccgaa
     1381 ggctggccaa gggtatcggg tggtcagtcc catcggaccg catttgtgat ctggaccccg
     1441 gcattcgccg aggtgcacgg agggaagggt taaagctgcg ccagcaggtg ctcggccgag
     1501 gacaccttga actcgcccgg ctgttcgatg ttcagctgtt cgaccacgcc gtccctgacc
     1561 agcatcgaat agcgctgcga gcgctcgccc atgccgaagc ccttggcgtc catcgacagg
     1621 cccagctggc gggtgaagtc gccgttgccg tcccccagca tgacgatctc gtccttgccg
     1681 atgccctgac tgtcggccca ggcgcccatg acgaaggcgt cattgaccga gacgcaggcg
     1741 atgatgtcca cgcccttgcc gacgatgtcc tcgcgcaggt ccttgaagcc cggcaggtgc
     1801 ttggccgagc aggtcggggt gaaggcgccc gggacggcga acagggccac ggtcttgccg
     1861 gcgaagacct cgtccgtcgt gaccggccgg gggccgtcgt cggtggcgcg ggtcaggttc
     1921 gccgagggaa tgcggtcgcc gatctggacg gtcatgggca ggctccggaa atggggtgag
     1981 gacgaaacgt gccgatcaga taggcgttgc cgctgctcgc gccaaccgtc ccgtctccga
     2041 ccgccttgcg aaggccgcgc tccatgtcga tgatagggtc atggacgaat tccaatccct
     2101 ggcggggcgg cttctggtgg ccatgcccgg catcagcgac ccccggttcg agcacgcggt
     2161 catcctgatc tgcgcccacc ggccggatca cgccatgggc ctgaggctgg accggcccgc
     2221 gccgggcgtg aacctgaaga aggtgctgac caagctggac acgcccctgc ccccggacgc
     2281 ggacagccgc gccgtgctga tcggcgggcc ggtggaccgc gagcggggct ttgtactgca
     2341 caccgacgac tggctggtcg acgacgcctc cttggcgttc ggcgccggct tcgccatgac
     2401 gggcacccga gaggccctgc tggccatggt cgatcccgag acggggccgc accgatctgt
     2461 gctgctgctc ggctacgctg gctggggcga tggccagctg gaggacgagc tgggcgagaa
     2521 cgtttggctg gtcgccgagg ccgatccgga gctgatcttc gacaccgacc acgagagcaa
     2581 atggggccgg gtgctggccg ggctgggcgt cgaccccgcg cagctgtcgg gacagatcgg
     2641 gcgggcctag cgacggggct aggccgaccg cgccttgatc ggcgccgtgc cgtcggccag
     2701 cgactcgttc agatagacct cggcctcctg agccggcagc gcctgcgcat aaccgaagcc
     2761 ttggccgtag tggcactggg cctcgagcag cagcttcgcg aggccggcgt tctcgacgcc
     2821 ctcggcgacc acgtccagcc ccagatcgcg gcccagattg accaccgact tgacgatctt
     2881 ggccgagccc tcgtccttgt ccatggtcag aacgaaatag cggtcgatct tcagcgtatc
     2941 gaacggcagc ttggccagcc aggtcaggga cgagaagccg gtgccgaaat cgtccagcgc
     3001 cagcgaagcg ccggcctcgc gcagccgcgt cagggtctcg gccgcgcgcg aggtgtcgcg
     3061 catgatatcg ccctcggtca cctccagctt cagcgccccc ttgggcaggc cggactcggt
     3121 aatgatgcgg gtgacgtcag cgaccagatc cggccgttcg atctcgccga ccgacagatt
     3181 gacgctgcag aacagctt
//
LOCUS       DASVVN010000599         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_129019,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000599 DASVVN010000000
VERSION     DASVVN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129019"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VF686_09770"
     CDS             complement(<1..814)
                     /locus_tag="VF686_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8662141.1"
                     /translation="MLTIENLTHVYGNGTRALDDVSLTIPKGMFGLLGPNGAGKSTLM
                     RSIATLQTPTSGSIRFGDIDVIKDPEQLRRVLGYLPQDFGVYPRVSAYDMLDHMAVLK
                     GVSNGKDRKQTVEALLNQVNLWPVRGKALAGFSGGMRQRFGIAQALIGNPELIIVDEP
                     TAGLDPEERNRFLNLLAEVGENVVVILSTHIVEDVTDLCPRMAVLAGGKIQLEGAPID
                     LMHTLEGRVWTKTIDKAELPEFTKRHNVISTRLFAGRTVVHVLSDANPGAGFE"
     gene            complement(903..>2283)
                     /locus_tag="VF686_09775"
     CDS             complement(903..>2283)
                     /locus_tag="VF686_09775"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX8662142.1"
                     /translation="SALSTIVDLEDSVAAVDAADKTAAYRNWLGLMKGDLSDTFDKGG
                     RSMTRALAPDRAFIAPNGSTVTLPGRSLLFVRNVGHLMTTPALRMADGTEAPEGVLDA
                     IVTSLIAVHDLKGQGRFRNSRAGSIYIVKPKMHGPEECAFTDALFDAVEDLLGLARHT
                     VKVGVMDEERRTSANLAACIHEVKDRIVFINTGFLDRTGDEIHTAMQAGPVVRKGEIK
                     SSAWIAAYEARNVAIGLKCGLSGRAQIGKGMWAAPDRMAEMLEQKAAHPKTGANTAWV
                     PSPTAATLHALHYHQTDVFALRDVIAARSVPDLADLMTPPLAAGRHWPEAEIAEELDN
                     NAQGLLGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHMANWLLHGVCTAEQV
                     DAALLRMAARVDAQNAGGPLYRPMASDPEGSLAFRAARALVFDGVAQPNGYTEPLLHQ
                     FRRRMKTAG"
BASE COUNT          352 a          767 c          765 g          399 t
ORIGIN      
        1 gctcgaaccc ggcgcccgga ttggcgtcgg acaggacgtg gacgacagtc cgccccgcga
       61 acaggcgggt cgagatgacg ttgtggcgct tggtgaactc gggcagttcg gccttgtcga
      121 tggtcttcgt ccagacccgg ccttcgagag tgtgcatcag gtcgatcggg gcgccttcca
      181 gctggatctt gccgccggcc agcaccgcca tgcgggggca cagatcggtc acatcctcga
      241 cgatgtgggt cgacaggatg accacgacgt tctcgcccac ctcggccaac aggttcagga
      301 aacggttgcg ctcttccggg tcgaggccgg cggtcggctc gtcgacgatg atcagttcgg
      361 ggttgccgat cagggcctgg gcgatgccga agcgctggcg catgccgccc gagaaaccgg
      421 ccagcgcctt gccgcgcacg ggccacagat taacctggtt caacagggcc tcgaccgttt
      481 gtttgcggtc cttgccgttg gagacgccct tcagcaccgc catgtgatcg agcatgtcgt
      541 aggccgagac gcgcggatag acgccgaaat cctgcggcag atagcccagc acgcggcgca
      601 gctgttcggg atccttgatc acgtcgatgt cgccgaagcg gatcgaaccc gaggtcggcg
      661 tctgcagggt ggcgatcgac cgcatcagcg tcgacttgcc cgcgccgttc gggcccagca
      721 ggccgaacat ccccttgggt atcgtcaggc tgacgtcgtc cagggcccgc gtgccgttgc
      781 cgtagacgtg ggtcaggttc tcgatcgtca gcatggaagc ccctccgtga agtaaaggaa
      841 ccttgtcagc ggcccctcgt actcgcaagt tacgaaacgg acatgtttgc cgtccagcgg
      901 gatcagcctg cggttttcat ccgtctacgg aactgatgca gcagcggctc ggtatagccg
      961 ttgggctgcg ccacgccgtc gaacaccagg gctctcgctg cgcggaaggc tagactgccc
     1021 tcggggtctg acgccatcgg gcggtacagc ggaccgcccg cgttctgggc atcgacccgc
     1081 gccgccatcc gcagcagcgc cgcatcgacc tgctccgccg tgcaaacgcc gtgcaggagc
     1141 cagttggcca tatgctggga cgagatgcgc agggtcgccc ggtcttccat caggccgacg
     1201 tcgtggatgt ccggcacctt ggaacacccc accccctgat cgatccagcg caccacatag
     1261 ccgagcagtc cctgcgcatt gttgtccagc tcctcggcga tctccgcctc cggccagtgt
     1321 cggcccgcgg ccagcggcgg ggtcatcaga tccgcgagat cgggaaccga ccgcgcggcg
     1381 atcacgtctc gcagggcgaa gacgtcggtc tggtgatagt gcagggcgtg cagggtcgcc
     1441 gccgtcggac tggggaccca agcggtgttg gcgccggttt tgggatgtgc ggccttctgc
     1501 tccagcattt cggccatccg gtctggcgcg gcccacatgc ccttgccgat ctgcgcccgg
     1561 cccgacaggc cgcacttcag gccgatggcg acgttgcgcg cctcatacgc ggcgatccag
     1621 gcggacgact tgatctcgcc cttccgcacc accgggccgg cctgcatggc cgtgtggatt
     1681 tcgtcgccgg tgcggtcgag gaagccggtg ttgatgaaga cgatccggtc cttcacctca
     1741 tggatgcagg cggcgaggtt ggccgaggtg cggcgctcct catccatcac cccgaccttg
     1801 accgtgtgcc gcgccagtcc cagcagatcc tcgacggcgt cgaacagagc gtcggtgaag
     1861 gcgcactcct cgggcccgtg catcttgggt ttgacgatgt agatcgagcc ggcgcggctg
     1921 ttgcggaacc gcccctgccc cttcagatcg tgcacggcga tcagcgaggt gacgatggcg
     1981 tccagcaccc cttcgggcgc ctcggttccg tcggccatcc gcagcgcggg cgtggtcatc
     2041 aggtgaccga cgttgcgcac gaacagcagc gaccggcccg gcagcgtcac ggtcgatcca
     2101 ttgggcgcga tgaacgcgcg gtccggcgcc agcgcccgcg tcatcgaccg cccgcccttg
     2161 tcgaaggtgt cgctgaggtc gcccttcatc aggcccagcc agttgcggta ggcggccgtc
     2221 ttgtcggcgg cgtcgacggc cgcgaccgag tcctccaggt cgacgatggt gctcagggcc
     2281 gac
//
LOCUS       DASVVN010000600         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_235125,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000600 DASVVN010000000
VERSION     DASVVN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235125"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1191)
                     /locus_tag="VF686_09780"
     CDS             complement(52..1191)
                     /locus_tag="VF686_09780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8662143.1"
                     /translation="MAALEVEIVAIDALGETRWALWRDMLAADPALNSPYFRPEFAQV
                     AARISPDAAVAVFTRGGDIVGFFPHQRRGGALQPLAAPMNDYHGVIAFPGQAPSLAEV
                     ARALGATRLNVTAWVGETDLGEDRRTVQVELGEGGYDAWYADRRANQGKFFKDKERAR
                     RSMEAELGPLRVERGLRDPALLDWLIDLKRDQYRRTSRHDIFACGWTADLLHALLKED
                     HDGFGASMAGLWAGDRLTAIEYSLHAGDQYHFWFPGYDPTLARCSPGILLSMDTMRLA
                     SELGYRTFDFGFEGEHYKKYFCNGARIVREAVVLKPGLGAAVSEAAVGVLGGRGERLR
                     TSVRRRWAAIEACETTSGARLKGAMQAAGAALAKARATSTRPVPA"
     gene            1353..1523
                     /locus_tag="VF686_09785"
     CDS             1353..1523
                     /locus_tag="VF686_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662144.1"
                     /translation="MTDTNRPPNRETENEDQRNPSRDGQPPRPATEPRGSEGSSNSGE
                     TATDPATGKPNP"
     gene            1583..>2018
                     /locus_tag="VF686_09790"
     CDS             1583..>2018
                     /locus_tag="VF686_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662145.1"
                     /translation="MSLRLIFASASLVVLAACGAPDPDQPDEAAPSAGAPNAPAAGNP
                     VLTVAQIEAGVPQVANAPGAAGPMPAPAPATGEAGSGPAATPPDAGLVRLQVLLDRTP
                     FSPGMIDGLAGDNTRQAIAAYRKANNLGSGEAADAALLQHLAA"
BASE COUNT          312 a          753 c          663 g          290 t
ORIGIN      
        1 cctgtttgat agggccggaa taacgggtgc gcgtatcggt catggagtgt ctcaggccgg
       61 aacgggtcgg gtcgatgtcg cccgggcctt tgcaagcgcc gcgcctgccg cctgcatcgc
      121 gcccttcaga cgggcgcccg aggtggtctc gcacgcctcg atggccgccc agcgccgccg
      181 cacactcgtg cgcagccgct cgccgcgtcc gcccagcacc ccgaccgccg cctcgctgac
      241 agcggcgccc aagccgggct tcagcaccac ggcctcccgc acgatgcgcg cgccgttgca
      301 gaaatacttt ttgtaatgct caccctcgaa tccgaagtcg aaggtgcgat agcccagttc
      361 cgacgccagc cgcatggtgt ccatgctcag cagaatgccc ggagagcacc gcgccagcgt
      421 gggatcatag cccgggaacc agaaatgata ctggtcgccc gcgtgcagcg aatattcgat
      481 cgcggtcagc cggtcgccgg cccacaggcc cgccatggag gcgccgaaac cgtcgtggtc
      541 ttccttcagc agggcgtgca gcaggtccgc cgtccagccg caggcgaaga tgtcgtgccg
      601 cgaggtgcgt ctgtactgat cccgcttcag atcgatcagc cagtccagca gggccggatc
      661 gcgcaggccg cgctcgaccc gcagggggcc cagctcggcc tccatgctgc gccgggcgcg
      721 ttccttgtcc ttgaagaatt tgccctggtt ggcgcggcgg tcagcgtacc aggcgtcata
      781 gccgccctcg cccagctcga cctggacggt gcgccggtct tcgccgagat cggtctcgcc
      841 aacccatgcc gtgacgttca gacgggtcgc gcccagcgcc cgcgcgacct ctgccagcga
      901 aggcgcctga cccgggaagg cgatgacgcc gtgatagtcg ttcatcggcg cggccagcgg
      961 ctgaagggcc ccgccccgcc gctgatgggg aaagaagccg acgatgtcgc cgccgcgcgt
     1021 gaacaccgcc acggctgcgt cggggctgat acgggccgcg acctgggcga attcgggccg
     1081 gaaatacggg ctgttcagcg ccggatcggc ggccagcatg tcgcgccaca acgcccaccg
     1141 ggtctctccc agcgcgtcaa tcgcaacgat ttccacctca agcgcagcca ttcgatcgtc
     1201 ccgcgacgcg tccctgtcgg acggcctcca accgcgccct tctaacccgc cgcccgtttc
     1261 gggccggtga gcgttcatgg tgaacggcag gtgaccggga gcctcgccgg tggcggggcg
     1321 ttgccattgg actgaccgtc gccggagttc ccatgaccga tacgaaccgt cccccgaacc
     1381 gcgagaccga gaacgaggac cagcgcaacc cgtcccgcga cggccagccg ccccggccgg
     1441 cgaccgaacc ccggggatcg gaaggctcgt cgaactccgg ggagacggcg accgaccccg
     1501 cgacaggcaa gccgaacccc tgagtggaac acgcggcgcg gtgggggatt gggtccggac
     1561 acatcaaaaa atccggattc ccatgtcgct tcgcctgatc ttcgcctccg ccagtctcgt
     1621 cgtcctcgcc gcctgcggcg cgcccgatcc ggaccagccc gatgaggctg cgccctcggc
     1681 cggggcgccg aacgcaccgg ccgccggcaa tcctgtcctg accgtcgccc agatcgaggc
     1741 cggtgttccg caggttgcga acgctccggg cgctgccggg cccatgcccg ctcccgcgcc
     1801 tgcaacgggc gaggccggat cgggtccggc cgcgacgccg cccgacgcgg gtctggtgcg
     1861 cctgcaggtg ctgctcgacc ggactccgtt ctcgcccggc atgatcgacg ggctcgcggg
     1921 cgacaacacg cgtcaggcca tcgcggccta tcgcaaggcc aacaacctcg gctcgggcga
     1981 ggccgccgac gccgccctgc tccagcatct cgccgccg
//
LOCUS       DASVVN010000601         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_259381,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000601 DASVVN010000000
VERSION     DASVVN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259381"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF686_09795"
     CDS             complement(<1..402)
                     /locus_tag="VF686_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8662146.1"
                     /translation="MTESSFMSAEELAAAGFGAVGRNCQVSRFARFYGASAVQLGDHV
                     RIDDFAIISAGEAVSLGDHVHIAPFCLLMGRSGITMEAFSGLGSRVAVYSESDDPSGR
                     SLVNPTIPRQYKPRYRAGAVRIGRHCGVGAGS"
     gene            complement(482..604)
                     /locus_tag="VF686_09800"
     CDS             complement(482..604)
                     /locus_tag="VF686_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662147.1"
                     /translation="MTDPKPPKTGTPPPKLEREARLAQALRANLKRRKAKPEPG"
     gene            complement(615..689)
                     /locus_tag="VF686_09805"
     tRNA            complement(615..689)
                     /locus_tag="VF686_09805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:ggt)
     gene            complement(755..>1616)
                     /locus_tag="VF686_09810"
     CDS             complement(755..>1616)
                     /locus_tag="VF686_09810"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX8662148.1"
                     /translation="CKAGVVGSFPALNARPESMLDEWIHQITEELAAWDRDHPETPAA
                     PFAVNQIVYRTNVRLQHDMEVCVKRKVPIVISSLGARPEVNDAVHSYGGVTLHDVINV
                     NHARKAIEKGADGVIAVAAGAGGHAGMQSPFALIQEIRAWFDGPLVLSGAIATGRAVL
                     AAQAAGADLGYIGSAFIATEEANASAAYKQAIVEGTASDIVYTRFFTGVHGNYLKSSI
                     VASGLDPDRLEGLDPTTMDFGAASGAKVWRDIWGSGQGIGAVDAVIPAAAFVDRLARE
                     YAEAKAALCG"
BASE COUNT          240 a          558 c          582 g          236 t
ORIGIN      
        1 cgagccggcg ccgacgccgc agtggcgtcc gatgcgcacg gcgccggcgc ggtagcgcgg
       61 cttgtactgg cgcgggatgg tcgggttgac cagcgatcgg ccagaggggt cgtcgctctc
      121 cgaatagacg gcgacgcgcg agcccagccc gctgaacgcc tccatggtga tgcccgagcg
      181 tcccatcagc aggcagaagg gggcgatgtg cacgtggtcg ccgaggctca ccgcctcgcc
      241 cgcggaaatg atcgcgaaat cgtcgatgcg gacatggtcg ccgagctgaa cggcggacgc
      301 cccgtagaag cgcgcgaagc ggctgacctg gcagttgcgc ccaacggcgc caaagccggc
      361 ggccgccagc tcctcggccg acatgaaact gctctcggtc acccgcgccc tcccgtcccg
      421 ggcgcaccat agccgcggcc ggcgggaaat cagctgttcg cccgcctccg gcgagcgggg
      481 gtcagcccgg ctcgggcttg gccttgcggc gcttgaggtt ggcccgcagc gcctgggcga
      541 ggcgcgcctc ccgctccaac ttgggcggag gggtcccggt tttcgggggc ttggggtcag
      601 tcatgagagg gtgctggtgc cgccggacag gatcgaactg tcgacctcag ccttaccaag
      661 gatgcgctct accactgagc tacggcggcg aagaggcgcc gatatagagg agcccgccgg
      721 gcagggaaag gccccgggac gcccggccgc agcctcagcc gcagagggcg gccttcgcct
      781 cggcgtactc gcgggcaagg cggtccacaa aggcggcggc gggaatgacg gcgtccaccg
      841 ccccgatccc ctgccccgag ccccagatgt cgcgccagac cttggccccg ctggcggcgc
      901 cgaagtccat ggtggtgggg tcgaggccct ccagccggtc cggatcgagg cccgaggcga
      961 cgatcgagct cttcaggtag ttcccgtgca cgccggtgaa gaaccgcgtg tagacgatgt
     1021 ccgatgcggt cccctcgacg atggcctgct tgtaggcggc gctcgcgttg gcctcttcgg
     1081 tggcgatgaa ggccgagccg atgtagccga ggtcggcgcc ggccgcctgc gcggccagca
     1141 ccgcacggcc ggtggcgatg gcgcccgaca gcaccagggg gccgtcgaac caggcgcgga
     1201 tctcctggat cagcgcaaac ggcgactgca tgcccgcatg gccgccggcg cccgccgcca
     1261 ccgcgatgac gccgtcggcg cccttctcga tcgccttgcg cgcatgattg acgttgatca
     1321 cgtcgtgcag cgtgacgccg ccgtagctgt gcaccgcatc gttcacttcc ggccgcgcgc
     1381 cgagcgagct gatgacgatg gggaccttgc gcttcacgca gacttccatg tcgtgctgaa
     1441 gccgcacgtt ggtgcggtag acgatctgat tgaccgcgaa cggcgccgcg ggcgtctccg
     1501 gatgatcgcg gtcccaggcc gccagctctt cggtgatttg gtggatccat tcgtcgagca
     1561 tcgactccgg gcgggcgttc agcgccggga aggagccgac cacgccggcc ttgcac
//
LOCUS       DASVVN010000602         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_498056,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000602 DASVVN010000000
VERSION     DASVVN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498056"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VF686_09815"
     CDS             complement(<1..879)
                     /locus_tag="VF686_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX8662149.1"
                     /translation="MNLDLYSDRAKQAVQSAQSLALARRHQQFAPEHLLKVLLEERDG
                     LARNLITAAGGDPARAETAVETALKKRAQVSGGSGQLYLDGDTARVFSSGEEASKKAG
                     DAFVTTERLLGAIAREGGVAAEVLKSVGATPEKLDAAIADIRKGKTADSAAAEDAYDA
                     LKRYARDLTLAARDGKIDPVIGRDEEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVEG
                     LALRIVNGDVPESLEDKKLLGLDMGALIAGAKYRGEFEERLKAVLNEVTAAEGGIVLF
                     IDEMHTLVGAGKSEGAM"
     gene            1045..>1562
                     /locus_tag="VF686_09820"
     CDS             1045..>1562
                     /locus_tag="VF686_09820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8662150.1"
                     /translation="MSIRLTRRAALVSSVAGAAALPSRGWAQAANDSAALADQVDAYV
                     KRVMAGFPDQPAVSVALVKGGQPVLTRGYGVRAIGGAAVDEHTLFAIASNTKAVTCAA
                     LAILIDEGKVKWDEPVRTYLPGFSLSKPELNEMVTVRDLVSHRIGFGLGAGDLLFWPN
                     SDRTRAEIMAAIP"
BASE COUNT          203 a          485 c          610 g          264 t
ORIGIN      
        1 catggcgcct tcggatttgc cggccccgac cagggtgtgc atctcgtcga tgaacaggac
       61 gatgccgccc tcggccgccg tgacctcgtt cagcaccgcc ttcaaccgct cctcgaactc
      121 gccgcggtac ttcgcgccgg ctatcagggc gcccatgtcc aggccgagca gcttcttgtc
      181 ctcgaggctt tcgggcacgt cgccgttgac gatgcgcagg gccaggccct cgacgatggc
      241 ggtcttgccg acgccgggct cgccgatcag gacggggttg ttcttggtgc ggcgggcgag
      301 gacctggatg gtgcggcgga tctcctcgtc gcggccgatg acggggtcga tcttgccgtc
      361 gcgggcggcc agagtcaggt cgcgggcgta gcgtttgagg gcgtcgtaag cgtcctcggc
      421 ggcggcgctg tcggcggtct tgcccttgcg gatgtctgcg atggcggcgt cgagcttttc
      481 gggcgtggcc ccgacggatt tcagcacctc ggccgcgacg ccgccctcgc gggcgatggc
      541 gccgagcagg cgttcggtgg tgacgaaggc gtcgccggcc ttcttcgagg cttcctcgcc
      601 gctggagaag acgcgggcgg tgtcgccgtc gagatagagc tggcccgagc cgcccgagac
      661 ctgggcgcgc ttcttgaggg cggtttcgac ggcggtttcg gcgcgggcgg ggtcaccgcc
      721 ggcggcggtg atcagattgc gggcgaggcc gtcgcgctct tccagcagga ccttgagcag
      781 gtgttcgggg gcgaactgct ggtggcggcg ggcgagcgcg aggctctgcg cggactggac
      841 ggcctgtttg gcgcggtcgg agtagaggtc gaggttcaag gcgtattccc tgcggacagc
      901 ggaggagccg ggcgcatcct catgcaagca acgcgccggg cggttgctga tcaggtgggc
      961 gcgtggggcg aagggtgcaa ggggcggctg gccttggccg gaaacgcgcg ataggtctga
     1021 caggacacgt ccgggagtct ctgcatgtcc atccgcctca cccgccgcgc ggcgctggtt
     1081 tcgtccgtgg ccggagcggc cgccctgccg tcgagggggt gggcccaggc cgccaacgac
     1141 agcgcggcac tggccgatca ggtcgatgcg tatgtgaagc gggtgatggc gggcttcccg
     1201 gatcagccgg cggtctcggt ggcgctggtc aagggcggtc agccggtgct gacgcggggc
     1261 tacggcgtgc gggccatcgg cggcgcggcg gtggacgagc acacattgtt cgccatcgcc
     1321 tcgaacacca aggcggtgac ctgcgcggca ctggccatcc tgatcgatga gggcaaggtg
     1381 aagtgggacg aaccggtgcg gacctatctg cccggcttca gcctgtcgaa gcccgagctg
     1441 aacgagatgg tgacggtgcg cgatctggtc agccaccgga tcggcttcgg gctgggcgcg
     1501 ggcgacctgc tgttctggcc gaacagcgac cggacccggg ccgagatcat ggcggcgatc
     1561 cc
//
LOCUS       DASVVN010000603         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_77312, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000603 DASVVN010000000
VERSION     DASVVN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77312"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VF686_09825"
     CDS             <1..1052
                     /locus_tag="VF686_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8662151.1"
                     /translation="LPAAARGARHGLFADAALEAGARVVLFAHTADDIAEGEVMRAEG
                     STLGRLRDWAPSPAWPEGRGLMLLRPMLDAGRAEVRKWLAGRGAEWIDDPANDDPQYA
                     RSRARTSLLPMGEGGPMGRMRVDRGSGEAAPRQPPLTLSRKNDGSAAVRSTPLPQGEG
                     WLVVDRDADATTLAAALVCAGGGDRPPRGDRLDGLITRLRLGQSFTATLCGARIEADG
                     DRAALFREPGELTRRPPSPLPLSPGVEAVWDGRWAITTDEPDWSVVRAAGHMAALSDT
                     DRVMLKALPPAARAGQPVLIRNVHGAPVLARRAARTRSLVEQRLALALDRMTHERDLG
                     AVFHGETLRNPLFST"
     gene            1095..>1542
                     /locus_tag="VF686_09830"
     CDS             1095..>1542
                     /locus_tag="VF686_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX8662152.1"
                     /translation="MNLRNFAIWGVIILGAMAFYVAVSRPGGIAAPANGKAAAERPQV
                     MTYSDLLKARDAGDITRVESRGETLKATLKDNRVVTVTTPTPNGPLLDSIAASGAQVD
                     AKSTRQSIWVSALIGLLPFVLIIGLWVLIMRQMQGGARGAMGFGKSK"
BASE COUNT          221 a          578 c          528 g          215 t
ORIGIN      
        1 gcctgcccgc cgccgcccgc ggcgcccggc atggcctgtt cgccgatgcg gcgctcgagg
       61 cgggagcccg ggtcgtcctg ttcgcccaca ccgccgacga catcgcggaa ggggaggtca
      121 tgcgcgcgga gggctcgacg ctgggtcggt tgcgggactg ggcgccatcg cccgcatggc
      181 ccgaaggacg cgggctgatg ctgctgcgcc ccatgctgga cgcgggccgg gccgaggtgc
      241 ggaagtggct tgcggggagg ggcgccgaat ggatcgacga cccggccaat gacgatccgc
      301 agtatgcccg ctcccgcgcc cgtacctccc ttctcccgat gggagaaggt ggcccgatgg
      361 gccggatgag ggtcgacagg ggaagcggcg aagccgcccc ccgacagccg cccctcaccc
      421 tttcgcgcaa gaacgacggc tccgccgccg tgcgctcaac ccctctcccg cagggagagg
      481 gctggcttgt ggtcgaccgc gatgccgacg ccacgaccct ggccgccgcc ctcgtctgcg
      541 ccggcggcgg cgaccgtccc ccgcgcggcg accggctcga cggccttatt acccgccttc
      601 gcttgggcca gtccttcacc gcgaccctct gcggcgcccg gatcgaggct gacggagacc
      661 gcgccgcgct cttccgcgaa cccggcgaac tgacccgacg cccgccgtcg cccttgccgc
      721 tgtcgccggg cgtcgaggcg gtctgggacg gtcgctgggc gatcacgacg gacgagcccg
      781 actggtccgt cgtccgcgcc gcgggccata tggccgccct gtccgacacc gaccgcgtca
      841 tgctcaaggc cctcccgccc gccgcgcgcg ccggccaacc tgtcctgatc cggaacgttc
      901 atggcgcccc ggttcttgca cggagggcgg cgaggacgcg gtcgctggtc gaacagagac
      961 tggcgcttgc actggaccga atgacgcacg aaagggacct gggcgccgtc tttcatggcg
     1021 aaaccctccg gaacccccta ttttcgacct gacatcagtg acggggcgtt ccgcgcccga
     1081 ggaacgagac gagtatgaac ctgcgcaatt tcgccatctg gggcgtcatc atcctgggcg
     1141 ccatggcctt ctatgtggcc gtcagccgac cgggcggcat cgccgcgccg gccaacggca
     1201 aggccgccgc agagcggccc caggtcatga cctattccga cctgcttaag gcgcgggacg
     1261 ccggcgacat cacccgcgtc gagtcgcgcg gcgagaccct gaaggccacg ctcaaggaca
     1321 accgggtcgt caccgtcacc acccccacgc ccaacggccc gctgctggac agcatcgccg
     1381 cctccggcgc ccaggtggac gccaagagca cgcgccagtc gatctgggtc agcgccctga
     1441 tcggcctgct gcccttcgtg ctgatcatcg gcctgtgggt gctgatcatg cgccagatgc
     1501 agggcggcgc gcgcggcgcc atgggcttcg gcaagtccaa gg
//
LOCUS       DASVVN010000604         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_313054,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000604 DASVVN010000000
VERSION     DASVVN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313054"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VF686_09835"
     CDS             <1..1176
                     /locus_tag="VF686_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8662153.1"
                     /translation="IDDFEALMLAMLLHDVGKGGDRGQLEDGAIAARRACDRLGLDPR
                     RTEFVVWLVRNHLALSDYAQKRDVSDPATVRAFTRLVGDPERLRTLLILTVADIRAVG
                     PGVWNAWKGNLIRDLYQRTEGVFRGEDMTHADPLDDYPDLVARARAEGAAAESVTVDG
                     SEEDERAATRVAVAARDRPGLFVDLADALAAAGADVVGARVATAPDGTALDVFELQDG
                     GGAPYGQAEPRRLTRLVEGLETAARGGMPTAPPPTPPPNPRRAAFDVRPTAMVDLDES
                     PTAAVVEVSGADRPGLLADLARAFAIHRLSIRSAHIAGFGERAVDSFYVTDAAGQKPK
                     PGPGLDALRADLETVLDVTERAGHRVSAPVRASLRDVSEVGRRGSAQEPVSRAPEPR"
     gene            1186..2895
                     /gene="murJ"
                     /locus_tag="VF686_09840"
     CDS             1186..2895
                     /gene="murJ"
                     /locus_tag="VF686_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX8662154.1"
                     /translation="MFRKTDVEAEPIEAAPPVPSAIATTDPMVQPPASGTTSRKGGGM
                     MRSSAIFSGLTLVSRLAGFARDIVISAALGASVGPAADAYNTALNFPNLFRRIFAEGA
                     FAAAFVPAYAKTLETEGEAVADKVATDALAAVAFATIALTIVAQLAMPWLMTVINVGF
                     LDDPARFKLAVVLTQITMPYLPCMAVAALLSGVLNARGRFIVSGAYPVLLNVIMLAAV
                     IPAQGGQIGAAYAASWAVLFAGVSQAGLCWWAARRAGADIRLALPRLTPGVKAIIITA
                     IPAAIGNSATQINVFISGNLSSFVAGGRTWLATADRLYQLPLGLVGVAIGIALLPRLS
                     RAVASEDHGQQQASMDEALTLSMALTLPAAAALMAMPYFLIDALFTRGAFLQVDAVNT
                     ARALFQFGWGVPAFVLIRILAPAFFARRDTRTPMIFALVSVAVNAALAIGLFNLGWGI
                     AGIAAAVSAAAWTNVILLAATLWRRGLYRPSPQALSRLTRIGLSAAGLALIVGLAAAS
                     RALIEAPVADALGVVGLDVGGKEAALLLVTAAGGLAYIALAFATRAITMAEVKGLLRR
                     TAK"
BASE COUNT          388 a         1158 c         1006 g          478 t
ORIGIN      
        1 atcgacgatt tcgaggccct gatgctggcc atgctgctgc acgatgtcgg caagggcggg
       61 gaccggggcc agctggagga cggagccatc gccgcaagac gggcctgcga ccggctgggg
      121 ctggatccgc gccgcaccga gttcgtcgtc tggctggtgc gcaatcacct ggcgctcagc
      181 gactacgccc agaagcgcga cgtctctgac ccggcgacgg tgcgcgcctt caccagactg
      241 gtcggcgatc cggagcggct gcgcaccctg ctgatcctga ccgtggccga tatccgcgct
      301 gtcgggccgg gggtctggaa cgcctggaag ggcaatctca tccgcgacct ctatcagcgc
      361 acggagggcg tgttccgggg cgaggacatg acccacgccg accccttgga cgactatccg
      421 gacctggtcg cccgggcccg agctgagggc gcggcggcgg aatcggtgac tgtcgacgga
      481 agcgaggaag acgagcgcgc cgccacccgt gtcgccgtcg cggcccgcga ccggcccggc
      541 ctgttcgtcg acctcgccga cgccctcgct gcggccgggg ccgacgtcgt cggcgcccgg
      601 gtcgccaccg cccccgacgg cacggccctg gacgtgttcg agcttcagga cggcggcggc
      661 gctccctatg gccaggccga gccgcgccgc ctgacccggc tggtcgaggg gctggagacg
      721 gccgcccgcg gcggcatgcc cacggccccg ccgccgaccc ctccgcccaa tccgcgccgc
      781 gccgccttcg acgtgcgccc caccgccatg gtcgatctgg acgaaagccc caccgccgcc
      841 gtggtcgagg tgtcgggggc cgaccgtccc ggtctgctgg ccgatctggc gcgggccttc
      901 gccatccatc ggctgtcgat ccgctcggcc catatcgccg gcttcggcga gcgcgcggtg
      961 gacagcttct acgtcacaga cgcggcgggc cagaagccta agcccgggcc tgggctggac
     1021 gccctgcgcg ccgatctgga gaccgtcctc gacgtcaccg aacgcgccgg ccaccgcgtc
     1081 tccgctccgg tccgggccag ccttcgcgac gtctccgagg tcgggcggcg aggctcggcg
     1141 caagagcccg tatcgcgcgc gccggaacca cgctaaggcg cttccatgtt cagaaagacc
     1201 gacgtcgagg ccgaaccgat cgaggccgcc ccgcccgtac catcggcgat cgcgacgacc
     1261 gatccgatgg tccagccgcc ggcctcgggc acgacctccc gcaagggcgg cggcatgatg
     1321 cgctcgtcgg cgatcttcag cggcctgacc ctggtcagcc ggctggccgg tttcgcccgc
     1381 gacatcgtga tctccgccgc ccttggggcg tcggtcggac cggcggcgga cgcctacaac
     1441 acggccctca acttccccaa tctgttccgg cgaattttcg ccgagggcgc cttcgccgcc
     1501 gccttcgtgc cggcctacgc caagaccctc gagaccgaag gcgaagcagt ggcggacaag
     1561 gtcgccaccg acgccctcgc cgccgtggcc ttcgccacca tcgccctgac catcgtcgcc
     1621 cagctggcca tgccgtggct gatgaccgtg atcaatgtcg gcttcctcga cgacccggcg
     1681 cgtttcaagc tggccgtcgt cctgacccag atcaccatgc cctatctgcc ctgcatggcc
     1741 gtggcggccc tgctcagcgg cgtcctgaac gcgcgcggtc ggttcatcgt ctcgggcgcc
     1801 tatccggtcc tgctcaacgt cattatgctg gccgccgtga tcccggccca gggcggccag
     1861 atcggcgccg cctacgccgc atcctgggcc gtgctcttcg ccggcgtctc gcaggccggg
     1921 ctgtgctggt gggccgcccg gcgcgccggc gccgacattc gtctggccct gccgcgcctg
     1981 acccccgggg tgaaggccat catcatcacc gccatccctg ccgcgatcgg caactcggcg
     2041 acgcagatca acgtcttcat ctcgggaaac ctgtccagct tcgtggccgg cgggcggacc
     2101 tggctggcga cggccgaccg cctctatcag ctgcccctcg gcctcgtcgg cgtcgccatc
     2161 ggcatcgccc ttctgccccg cctgtcccgc gccgtggcct ccgaggacca tggtcagcag
     2221 caggcctcga tggacgaagc cctgaccctg tccatggccc tgacgctgcc ggcggcggca
     2281 gccctgatgg ccatgcccta tttcttgatc gacgccctgt tcacccgagg agccttcctc
     2341 caggtcgacg ccgtcaacac ggcgcgggcc ctgttccagt tcggctgggg cgtgccggcc
     2401 ttcgttctga ttcgcatcct cgcaccggcc ttcttcgcgc ggcgggatac gcgcacgccg
     2461 atgatcttcg ccctcgtctc agtcgcggtg aacgccgcgc tggccattgg cctgttcaat
     2521 ctcggctggg gcatcgcggg catcgcggcg gcggtttccg ccgcggcctg gacgaatgtc
     2581 atcctgctgg ccgcgacgtt gtggcggcgc ggtctttacc ggcccagccc gcaagccctg
     2641 tcgcgcctga cgcgcatcgg actgtctgcg gcgggactgg ccctgatcgt cggcctggcc
     2701 gcggcgagcc gcgccctgat cgaggcgccg gtcgccgacg ccctcggcgt ggtgggtctg
     2761 gatgtcggcg gcaaggaggc cgccctgctg ctggtcaccg ccgccggagg actggcctac
     2821 atcgccctcg ccttcgcgac ccgcgccatc acgatggccg aagtgaaggg gctgctgcga
     2881 cgaacagcca agtaacggct tgctccttcg gcgatggtcg ggcattgcgc ctcgacatga
     2941 ccgacgcctc ccccgccgtt cccgtctatc aaggcccccg ccgcgtgctg agcggcatcc
     3001 aagcctccgg cgccctgcat ctgggcaact
//
LOCUS       DASVVN010000605         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_470246,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000605 DASVVN010000000
VERSION     DASVVN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470246"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF686_09845"
     CDS             complement(<1..261)
                     /locus_tag="VF686_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8662155.1"
                     /translation="MSGLTKTVKKGRPLLTLFLLLLLLGSGSIVLQRFLSPPATFLMT
                     SRLIEGEGLDYRWRSLDRISPRLVQAVIASEDSTFCAHRGFDM"
     gene            complement(302..1267)
                     /locus_tag="VF686_09850"
     CDS             complement(302..1267)
                     /locus_tag="VF686_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8662156.1"
                     /translation="MKTTDLDAQITAALFDRTGAIFALGDGSVRFEGGEFSAAHDGAI
                     LCAVVHPSGDGVLTGGDDGRLIWHRRGEAGVLATAKGQWIDAVDASAESGLIAFSAGK
                     TLSVIDAKDPAFRRDFQHERTVSGVAFEPKGRRIATSTYGGAALWFARIAQQKPTFLK
                     WAGSHTAVTFSPDGAFVVTAMQDAQLHGWRLKDQKNLRMGGYPSKVRAMSFLSNGQLL
                     ATSGAQGAVLWPFIGANGPMGREATEIGFDEGSLVALVASQPKHGLLAAGLNDGRVWL
                     ADPAGQGLNFLKAERGAPIVALAMSADATRVAWADEDGQAGVATI"
     gene            complement(1264..2349)
                     /locus_tag="VF686_09855"
     CDS             complement(1264..2349)
                     /locus_tag="VF686_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8662157.1"
                     /translation="MDRMNDKTPVTVLTGYLGAGKTTLLNRILTEDHGKRYAVIVNEF
                     GEIGIDNDLVVGADEDVFEMNNGCVCCTVRGDLIRVVAGLMKRQRPGKPAFDAIIVET
                     TGLADPGPVAQTFFVDEDVKAKTQLDSVTALVDAKHVMARLDDSKEAREQVAFADRII
                     LNKVDLVSEAELIEVERRLRKLNPLAPITRAERANVPLDQVLGLGGFDLGRILDINPD
                     FANPAHGEAGHVHDEHCGHDHHDHDHDHAHHDHDHHHGARGHAHEDDIKGISLSLERP
                     MDGTKFTAWLDRLLGEQGQNILRAKGIIDVNGEDRRLVFQAVHMILEGDLQREWGANE
                     RRWSRAVFIGRDLDEAALKAGFEACAA"
BASE COUNT          368 a          763 c          868 g          407 t
ORIGIN      
        1 catgtcgaag ccccggtgag cgcagaacgt ggagtcctcg gaggcgatca ccgcctgcac
       61 cagccggggc gaaatccggt cgagcgagcg ccagcggtag tcgaggccct cgccctcgat
      121 caggcggctg gtcatcagga aggttgccgg cggcgagagg aaacgttgca gcacgatgct
      181 gcccgacccc agcaggagca gcagcaggaa cagggtcagc agaggccggc ccttcttcac
      241 ggtcttggtc agccccgaca tggggggatc gcagcacgcc gggcggcggc ttggcaagcg
      301 atcagatcgt ggcgaccccg gcctgtccgt cttcgtccgc ccaggcgacg cgcgtggcgt
      361 cggcgctcat ggccagggcg acgatcggcg cgccgcgttc ggccttgagg aagttcaggc
      421 cctgacccgc cggatcggcc agccagacgc ggccgtcgtt cagacccgcg gccagcaggc
      481 cgtgcttcgg ctgcgaggcg accagggcga cgaggctgcc ttcgtcgaag ccgatctcgg
      541 tcgcctcgcg ccccatgggc ccgttggcgc cgatgaaggg ccagagaacg gccccttgcg
      601 cgcccgaggt ggccagcagc tggccgttgg acaggaagct catggcccgg accttggacg
      661 gataaccgcc catgcgcagg ttcttctgat ccttcagtcg ccagccgtgc agctgggcgt
      721 cctgcatggc ggtgaccacg aacgcgccgt cgggcgaaaa ggtcacggcg gtgtgcgaac
      781 cggcccattt gaggaaggtc ggcttctgct gggcgatgcg ggcgaaccac agggccgcgc
      841 cgccgtaggt cgaggtcgcg atgcgccggc ccttgggttc gaaggcgacg ccggagacgg
      901 tgcgttcgtg ctggaagtcg cggcggaagg cggggtcctt cgcatcgatg acgctcaggg
      961 tcttgccggc cgagaaggcg atcaggccgc tttcggccga ggcgtcgacg gcgtcgatcc
     1021 actggccttt tgccgtggcg agcacgccgg cctcgccccg acggtgccag atcaggcggc
     1081 cgtcgtcgcc gccggtcagg acgccgtcgc cggaagggtg gaccacggcg cacaggatgg
     1141 cgccgtcatg agcggcgctg aactcaccgc cctcgaaacg gactgagccg tcgccgaggg
     1201 cgaagatcgc tcccgtccgg tcgaacaggg ccgcggtgat ctgggcgtct aaatctgtgg
     1261 tcttcacgcc gcgcaggcct cgaacccggc cttcagcgcg gcctcgtcca gatcccggcc
     1321 gatgaagacg gcgcggctcc atcggcgctc gttggcgccc cactcacgct gcaggtcgcc
     1381 ttcgaggatc atgtgcacgg cctggaagac cagccggcga tcctcgccgt tgacgtcgat
     1441 gatgcccttg gcgcgcaaga tgttctgacc ctgctcgccc agcagccggt cgagccatgc
     1501 cgtgaatttc gtgccgtcca tcgggcgttc gagggaaagg gatatgccct tgatgtcgtc
     1561 ctcgtgggcg tgaccgcggg cgccatggtg atggtcgtgg tcgtgatggg cgtgatcatg
     1621 gtcgtgatcg tgatggtcgt ggccgcagtg ttcgtcgtgg acgtgcccgg cctcgccatg
     1681 ggcgggattg gcgaaatcgg gattgatgtc gaggatccgc ccgaggtcga aaccgccgag
     1741 gcccagcacc tggtccagcg gcacattggc gcgctcggcg cgggtgatgg gagccagcgg
     1801 gttcagcttg cgcagccggc gctcgacctc gatcagttcg gcctcggaga ccagatcgac
     1861 cttgttgagg atgatgcggt cggcgaaggc gacctgctcg cgcgcctcct tcgaatcgtc
     1921 gaggcgggcc atgacgtgtt tggcgtcgac caaggccgtt acgctgtcca gctgggtctt
     1981 ggccttgacg tcctcgtcga cgaagaaggt ctgggccacc gggccggggt cggcgaggcc
     2041 ggtggtctcg acgatgatgg cgtcgaaggc gggcttgccg ggacgctggc gcttcatcag
     2101 gccggcgacc acgcggatca ggtcgccgcg cacggtgcag cagacgcagc cgttgttcat
     2161 ctcgaacacg tcctcgtcgg cgccgaccac cagatcgttg tcgatgccga tctcgccgaa
     2221 ctcgttgacg atgacggcgt agcgcttgcc gtggtcctcg gtgaggatgc ggttcagcag
     2281 ggtggtcttg ccggcgccga gataaccggt caggacggtg acgggggtct tgtcgttcat
     2341 ccggtccatg taggccggcg gcgtccactt atgaagcctg gcgttctcaa tcgtaaatgt
     2401 tataaa
//
LOCUS       DASVVN010000606         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_259505,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000606 DASVVN010000000
VERSION     DASVVN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259505"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1601
                     /locus_tag="VF686_09860"
     CDS             <1..>1601
                     /locus_tag="VF686_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662158.1"
                     /translation="KGPKKAILNAASNSRPGDYLRVNAALGEKITDLRLNLRANEAQG
                     GPIAGALGYSPDRPFFATAVVNGSVVDAMVRTGDFVPLVIKGRYGDNRTRIAGFADFS
                     GSDLLAPFAERVGRTVKFGFATSTDSDRDGLHGVGWRLIADNLNASASGDVRLSDRSS
                     PDGIALDISTPSLTRLVGQRAAGPAAWKGVFKGDASSWTLTGSADLLGVELSSYRAQR
                     IRGPLNARMRLGRLDLDGDIRAAGGSSAGIVGGLLGSQPRVTFEAARLADGAILLERV
                     QATGQALTLSGSGGRSLTGGLGFRGQAQVTDVSRLRKGARGAFGGPITASMARAGQPW
                     RITFDAHGRRLATGMAELDRLLGPTPRLQATGALRGGRIEIERAALTGAQGTAGARGL
                     IEGDGRLRLALNWNARGPFGVGPVAIDGAMTGDGALTGTLARPRADLTAKFGKLSAGA
                     LVLTDADMILSFRRGADASDGRIALTAGSNYGPARASGNFFLGGDRIRLTDVDLNAGG
                     VTAQGAIALSNSFPSSADLTFTARPG"
BASE COUNT          232 a          592 c          557 g          220 t
ORIGIN      
        1 aagggtccga agaaggccat cctgaacgcg gccagcaaca gccggccggg cgactatctg
       61 cgcgtcaacg ccgcgctcgg cgagaagatc accgatctgc gcctcaacct gcgcgccaac
      121 gaagcacagg gcggaccgat cgcgggcgct ctgggctatt cacccgatcg gccgttcttc
      181 gcgacggccg tggtcaacgg ctcggtcgtc gacgccatgg tgcgtacggg cgacttcgta
      241 ccgctggtga tcaagggccg ttacggcgac aatcgcaccc gcatcgccgg cttcgccgac
      301 ttctcgggat cggacctgct ggcgccgttc gccgagcgcg tcggccgcac cgtcaaattc
      361 ggtttcgcca cttcgaccga cagcgaccgg gacgggctgc acggcgtcgg ctggcggttg
      421 atcgccgaca atctgaacgc cagcgccagc ggcgacgtcc gcctcagcga ccgcagcagt
      481 cccgatggga tagcgctgga catctcgaca ccgtccctga cccggctcgt tggccagcgc
      541 gcagcggggc cggccgcctg gaagggcgtg ttcaagggcg acgcgtcctc ctggaccctg
      601 accggatcgg cggacctgct gggggtcgag ctttcaagct atcgcgccca gcgtattcgc
      661 ggaccgctga acgcccgcat gcggctcggg cggctcgacc tggacggcga catccgcgcc
      721 gccgggggct ccagcgccgg cattgtcggc ggactgctgg gaagccagcc gcgcgtcacc
      781 ttcgaagcgg cccggctggc cgacggcgcc atcctgctgg agcgggttca ggcgactggc
      841 caggccctga ccttgtccgg ctcgggcgga cgcagcctga ccggcggtct cggtttccgc
      901 ggtcaagccc aggtcaccga tgtctcccgc ctgcgcaagg gcgcgcgcgg tgcgttcggc
      961 ggcccgatca cggcctcgat ggcgcgcgcc ggccagccgt ggcggatcac attcgacgcc
     1021 cacggccgcc ggctggccac tggcatggcc gaactggacc gcctcctcgg cccgacgccc
     1081 cggcttcagg cgaccggcgc tctcaggggc gggcggatcg agatagagcg ggcggcgctg
     1141 accggcgccc agggaacggc gggcgctcgc ggcctgatcg agggcgacgg acggctgcga
     1201 ctggccctga actggaacgc ccgagggccg ttcggcgtcg gcccggtcgc catcgacggc
     1261 gcgatgaccg gcgacggcgc cctgaccggc accctggccc gaccgcgcgc cgatctgacc
     1321 gcgaaattcg gcaagctgag cgccggggct ctggtcctga ccgacgcgga catgattctc
     1381 agcttccggc gcggcgcgga cgcctccgac ggccgcatcg ccctgaccgc cggatcgaac
     1441 tacggccccg cccgcgccag cggaaacttc ttcctcggag gcgatcgcat ccgcctgacg
     1501 gacgtcgacc tgaacgccgg cggcgtcacg gcgcaaggcg cgatcgccct gtccaacagc
     1561 ttcccctcga gcgctgacct gaccttcacc gcgcgaccag g
//
LOCUS       DASVVN010000607         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_289113,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000607 DASVVN010000000
VERSION     DASVVN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289113"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /gene="tpiA"
                     /locus_tag="VF686_09865"
     CDS             <1..716
                     /gene="tpiA"
                     /locus_tag="VF686_09865"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX8662159.1"
                     /translation="KMNGLGASLGEVEALRVALDKEPAACRVALCPPATLVERMAAAA
                     AGSPIEIGGQDCRSETHGAFTGDISAPMLKDAGATLVILGHSERRQGHGETDLLVSAK
                     AEAALAAGLAPIVCVGETLEQREAGRAVEVVRSQIMASLPLALAGRDFAVAYEPVWAI
                     GTGLTPTLEQIEEVHRAVRAAMIERLGLGVAATPILYGGSVKPDNAAEILAVPEVGGA
                     LVGGASLKAVDFLGIIRGA"
     gene            804..1217
                     /gene="secG"
                     /locus_tag="VF686_09870"
     CDS             804..1217
                     /gene="secG"
                     /locus_tag="VF686_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX8662160.1"
                     /translation="MLQTILLVAMILISVALAGVILIQRSEGGALGMGGGPSGFMTAR
                     GAGNLLTVTTWWLAGAFFACAIGLTILGNVERSRSSIVDADSIGELIAPTTPQTAPVA
                     DPSAPAQPAAPSLQDLESSLSGTAAPAPAQPAPAQ"
     gene            1307..>1859
                     /locus_tag="VF686_09875"
     CDS             1307..>1859
                     /locus_tag="VF686_09875"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8662161.1"
                     /translation="MARYVFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVSAAKSDNITTGRIYSTILEKE
                     RRGDYLGATVQVIPHVTDQIKSFCLTPALTDHGEDVDFVLVEIGGTVGDIEGLPFFEA
                     IRQLGNDLPRGQACYLHLTLMPYI"
BASE COUNT          281 a          636 c          629 g          313 t
ORIGIN      
        1 ggaagatgaa cggcctgggc gcgagtctgg gcgaggttga ggcgcttcgc gtcgcgctgg
       61 acaaggagcc cgcggcctgc cgcgtcgccc tgtgcccgcc cgcaacgctg gtggaacgca
      121 tggcggctgc ggcggccgga agcccgatcg agatcggcgg gcaggattgc cggtccgaga
      181 cccacggcgc cttcaccggc gacatctccg cgccgatgct gaaggacgct ggggcgaccc
      241 tggtgatcct gggccattcc gagcgtcgtc agggccatgg ggaaaccgat ctgctggtgt
      301 cggccaaggc cgaggccgca ctggcggcag gtctggcccc cattgtgtgc gtcggggaaa
      361 ctctggagca gcgcgaggca ggacgcgcgg tcgaggtcgt gcggtcgcag atcatggcct
      421 ccctgccgct ggcgctggcg ggccgcgact tcgcggtcgc ctatgagccg gtctgggcga
      481 tcgggacggg actgacgccg acgctggagc agatcgagga agtccaccgc gccgtccggg
      541 cggcgatgat cgaacgtctg gggctgggtg tcgcggcaac gccgatcctg tacggcggct
      601 cggtgaaacc ggacaacgcg gctgaaatcc tggcggtccc cgaggtcggc ggggcgctgg
      661 tgggcggggc gtccctgaag gcggtcgact tcctcggcat catccggggc gcctgagccg
      721 ccggagtgtt tgctcctgtc ccgcatcaat gttagacgcc gccgcttgat cggcgcggtg
      781 tcgcgccaca catatcggct gccatgctcc agaccattct cctcgtcgcc atgatcctca
      841 tttcggtcgc gcttgccggc gtgatcctga ttcagcgctc cgaaggcggc gcgctgggca
      901 tgggcggagg cccgtcgggc ttcatgacgg cgcgcggcgc gggcaatctg ctgacggtga
      961 cgacctggtg gctggctggc gccttcttcg cctgcgccat cggcctgacc atcctcggca
     1021 acgtcgagcg ttcgagaagc tcgatcgtcg acgccgactc catcggcgag ctgatcgctc
     1081 cgaccacgcc gcagacggct ccggtcgccg atccttcggc cccggctcaa cccgccgcgc
     1141 cgtcgctgca ggatcttgag tcgagcctgt cgggaacggc ggctcccgct cccgcccagc
     1201 ccgcgccggc gcagtaagga tgagcgggga ccttatcccc gccttccgct cccgccctca
     1261 aagtctccag tccgccgccc ccgaatcggc ggtaaggtaa tctcccatgg cccgttacgt
     1321 gttcatcacc ggcggcgtgg tttcctcatt gggaaaaggc ctcgcatccg ccgctctcgg
     1381 cgcgcttctg caagcgcgcg gctacaaggt tcgccttcgt aagctcgacc cctatctgaa
     1441 cgtcgatccg ggcacgatga gcccgtatca gcacggcgag gtcttcgtga ccgacgacgg
     1501 ggccgagacc gatctcgacc tgggccacta cgagcgcttc acgggcgtgt cggcggcgaa
     1561 gtcggacaac atcaccaccg gccgcatcta ttcgaccatc ctcgagaagg agcggcgagg
     1621 ggactatctg ggcgccacgg tgcaggtgat cccgcacgtc acggaccaga tcaaatcctt
     1681 ctgcctcacg cccgccctga cggaccacgg cgaggacgtg gacttcgttc tggtcgagat
     1741 cggcgggacc gtcggcgaca tcgaaggcct gccgttcttc gaggccatcc gccaactcgg
     1801 caacgatcta cccagagggc aggcctgcta tctacatctc accctgatgc cgtacattc
//
LOCUS       DASVVN010000608         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_313238,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000608 DASVVN010000000
VERSION     DASVVN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313238"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VF686_09880"
     CDS             complement(<1..943)
                     /locus_tag="VF686_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8662162.1"
                     /translation="MTRLTLAASAAALLAACLPLTAAAQNAPRPTVLIHAGNLFDSEA
                     GRMVGPRDILIRGERVAEVASSIALPEGATVLDLRGCSVLPGLIDAHTHLLLEQRSGE
                     GLSDVAARDQGVHGDVYRTLSGAQRAREYLEAGFTAVRDLGNSGQFLDLMLERGINDG
                     RVAGPRIYGSGPGLAPAGGQLEPQPADPHELVAGEYRIINGAADARAAVRQAIARGAD
                     VIKMYPEATPQRTRLSVEEMAAIVSEAKRHNVPVAAHATGDAAIREAVEAGVASIEHA
                     YEVSDDTLRLMASKGVWLVPTDPSLEMALTFVASWPQQ"
     gene            1060..1908
                     /locus_tag="VF686_09885"
     CDS             1060..1908
                     /locus_tag="VF686_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8662163.1"
                     /translation="MTASVLNDPSALAKAAEAGDGAAAARLAVLTGMGLGVPPDWRRA
                     MELLGRAADLGFTPARDQVALLAGRDGGIDIAAWLNAPRPRVLSEGPAIFAIDGFLSP
                     AVCDWMVARAEAMVEPALVYDHQTGTGRRESVRTNTAAQFDLEKMDVVLALVRERIAR
                     AAGLPVPGLEWTQVLHYDVGQTFDWHVDWLDPATPGYAADLAARGQRIATFLVFLNDD
                     FEGGETAFEAGGLRHRGRKGDALLWANTLPDGVIDRRTRHAGLPPTKGEKWVLSQWLR
                     GRAPRV"
     gene            complement(1915..2214)
                     /locus_tag="VF686_09890"
     CDS             complement(1915..2214)
                     /locus_tag="VF686_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX8662164.1"
                     /translation="MNPLMAAKAYAAIQDGASPVGGGGAVGQSGFADMLTSVMGDMTQ
                     SSRAAETQMASAVQGQGSLIDVVTAVSSAEASLETVMAVRDQVIQAYQEIMRMPI"
     gene            complement(2225..>2374)
                     /locus_tag="VF686_09895"
     CDS             complement(2225..>2374)
                     /locus_tag="VF686_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX8662165.1"
                     /translation="AEGYVLRPNVDTLVEAMDMREAQRAYEANLNIIETARSMDSRTL
                     DLLKK"
BASE COUNT          356 a          786 c          873 g          359 t
ORIGIN      
        1 gctgctgcgg ccatgaggcg acgaaggtca gggccatctc cagcgaaggg tcggtcggca
       61 ccagccagac gcccttcgac gccatcagac gcagggtgtc gtcggacacc tcataggcgt
      121 gctcgatgga ggcgaccccg gcctcgaccg cctcccggat ggcggcgtcg cccgtggcgt
      181 gggcggcgac cgggacgttg tggcgcttcg cttccgaaac gatggccgcc atctcctcga
      241 ccgacaggcg agtgcgctga ggcgtggcct ccgggtacat cttgatgacg tctgcccccc
      301 gggcgatggc ttggcgcacg gcggcgcgag cgtcggcggc gccgttgatg atgcggtact
      361 cgccggcgac aagttcgtgc ggatccgccg gctgcggctc gagctggccg cccgccggcg
      421 ccagaccggg gccggaaccg tagatgcgcg ggccggccac gcggccgtcg ttgattccgc
      481 gctccagcat cagatcgagg aactggccgg agttgccgag gtcgcgcacg gcagtgaagc
      541 cggcctcaag atactcgcgg gcgcgctggg ctccggacag ggtgcggtag acgtcgccat
      601 ggacgccctg atcgcgggcg gcgacatcgg acagaccctc tccgctgcgt tgttcgagca
      661 gcagatgcgt atgggcgtcg atcagaccgg ggagcaccga acagccacgc aggtccagaa
      721 ccgtcgcgcc ttcgggcaac gcaatggacg atgcgacctc ggccacccgc tcaccccgga
      781 tgaggatgtc gcgcggaccc accatccgtc cggcctcgga atcgaacagg tttccggcat
      841 ggatcagcac cgtcgggcgc ggggcgttct gcgctgcggc ggtgagcggc agacaggcgg
      901 ccagcagggc ggcggccgag gcggcgaggg tcaggcgggt catacggtct ccgatcggtt
      961 tctccagaca gaggctggca gaacctcgac cggatgcgaa gccgtcgatg aaatctccgt
     1021 aagcttggcc tgctccgtcg accgacctag aacagagccg tgaccgcttc agttctcaac
     1081 gacccgtccg ccctcgccaa ggccgccgaa gccggagacg gtgcggcggc ggcgcggctg
     1141 gctgtgctga ccggcatggg gctgggcgta ccgccggact ggcgccgggc gatggaactg
     1201 cttggccggg cggccgacct ggggttcacg cccgcgcggg accaggtcgc gctgctcgcc
     1261 ggccgcgacg gcgggatcga catcgccgcc tggctgaacg cgccgcggcc gcgggtcctg
     1321 tccgaaggtc cggccatctt cgctatcgac gggtttctgt ctccggcggt ctgcgactgg
     1381 atggtcgccc gggccgaagc catggtggag ccggccctcg tctatgacca tcagacgggc
     1441 acgggacggc gcgagagcgt gcgcacaaat acggcggcgc agtttgacct cgagaagatg
     1501 gacgtggtgc tggcgctggt gcgcgagcgg atcgcgcggg cggcggggct gccggttccg
     1561 gggctcgaat ggactcaggt gctgcactat gacgtgggcc agacgttcga ctggcatgtg
     1621 gactggctgg atccggccac gcccggctac gccgccgatc tggcggcgcg ggggcagcgg
     1681 atcgccacgt ttctggtctt cctgaacgac gatttcgagg gcggggagac cgcgttcgaa
     1741 gcgggcggcc tgcgccaccg gggtcgcaag ggcgacgccc tgctgtgggc caacaccctg
     1801 cccgacggcg tgatcgaccg gcggacgcgg cacgcgggcc tgccgccgac gaagggcgag
     1861 aaatgggtgc tgtcgcagtg gctcagaggg cgggcgccga gggtctgaca gaccctagat
     1921 cggcatccgc atgatctcct gataggcctg gatcacctga tcgcggacgg ccatgacggt
     1981 ttccagcgag gcctcggccg aggacacggc ggtgaccacg tcgatgagac tgccctgccc
     2041 ctgcaccgcc gacgccatct gggtctcggc cgcgcgcgag ctctgggtca tgtcgcccat
     2101 gacgctggtc agcatgtcgg cgaagccgga ctggcccacc gcgccgccgc caccgacggg
     2161 cgaggcgccg tcctggatcg ccgcataggc cttggccgcc atcaaggggt tcatcgctca
     2221 ggccctattt cttcagcagg tcgagggtgc ggctgtccat cgagcgcgcg gtctcgatga
     2281 tgttcagatt ggcctcgtag gcgcgctggg cctcgcgcat gtccatggcc tcgaccaagg
     2341 tgtcgacgtt gggacgcagc acataaccct cggc
//
LOCUS       DASVVN010000609         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_183750,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000609 DASVVN010000000
VERSION     DASVVN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183750"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF686_09900"
     CDS             complement(<1..511)
                     /locus_tag="VF686_09900"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX8662166.1"
                     /translation="MAFTLPPLPYAYDALEPAIDKETMTFHHDKHHQAYVDNLNKAVD
                     AEASLQGKTLEELFASISKAPKAVRNNGGGHWNHSLFWELLAPVGQTGEPSAELAAAI
                     DRDLGGMDKFKEAFNAAGAGQFGSGWAWLIIQDGKLQVTSTPNQDNPLMDVADEKGAV
                     VLAADVWEHA"
     gene            complement(659..889)
                     /locus_tag="VF686_09905"
     CDS             complement(659..889)
                     /locus_tag="VF686_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662167.1"
                     /translation="MTENSVPDYAPMFAALCVEVGFCLHSKGEAKVQAALANGLDAAV
                     RAVFDAEGVDFASSTGDLRRAVRDCLKAHLPA"
     gene            complement(909..1382)
                     /locus_tag="VF686_09910"
     CDS             complement(909..1382)
                     /locus_tag="VF686_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662168.1"
                     /translation="MTTTLTYPAEAFDDHPIITGRGADERVTSVTAFTGQSLEGPDGA
                     TVLLPMTVGARESRDLPTLQALADRIGPSGEGGGGTEAALAELSSPALPAQGLAFDHG
                     RGRVVVLGEAGMLSAQLIRFPPPSGRPDQRFGMNVAPGNAQFGLNIMHWLTGLLP"
     gene            complement(1342..1869)
                     /locus_tag="VF686_09915"
     CDS             complement(1342..1869)
                     /locus_tag="VF686_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX8662169.1"
                     /translation="MNRFALTLGLVAIAFAAPVAALQITDPNFHPTVARPAFAAEGPV
                     IVIDGAHNNFHTVDGRYAPFAALLRADGYQVRGGTAAFDAGGLEGADVLVIANAGPVD
                     GGSPFTASEIEAVETWVRSGGRLLLIADHAPFGTAAEALAARFGVRMGKGYAFAMTTA
                     RRIDDHPDLPRRGLR"
BASE COUNT          317 a          684 c          644 g          318 t
ORIGIN      
        1 aggcgtgttc ccagacatcg gcggcaagga ctacggcgcc cttctcgtcg gcgacgtcca
       61 tcagcggatt gtcctggttg ggcgtcgagg tcacctgcag cttgccgtcc tgaatgatca
      121 gccaggccca gcccgagccg aactggcccg cgcccgcggc gttgaaggct tccttgaact
      181 tgtccatccc gcccagatcg cggtcgatgg cggcggccag ttcggccgag ggttctccgg
      241 tctggccgac gggagccagc agttcccaga acaggctatg gttccagtgt ccgccgccgt
      301 tgttgcggac cgccttgggc gccttcgaga tcgaggcgaa cagctcctcc agcgtcttgc
      361 cctgcaggct ggcctcggcg tccaccgcct tgttcagatt gtcgacatag gcctgatgat
      421 gcttgtcgtg gtggaaagtc atcgtctcct tgtcgatggc cggctccagc gcgtcatagg
      481 cgtaagggag gggcggaagc gtgaaggcca tggggaggct ccttgcgacg gggttcgagg
      541 gagcccgtat ctaggcgccc gttcgccgaa cccaagcagt acggcgcgat ttcgttccgt
      601 ctcctccccc gccgggggag gggaccacgt agcggtggag ggggagccgg cctcaggctc
      661 aggcaggcag atgcgccttc agacagtcgc gcacggcccg acgcagatcg ccggtcgagg
      721 aggcgaagtc cacgccctcg gcgtcgaaca cggcccgaac cgctgcatcc agcccgttgg
      781 ccaacgccgc ctgtaccttc gcctcgccct tggagtgcag gcagaagccg acctccacgc
      841 acagggcggc gaacatcggg gcgtagtcgg gaacggagtt ttcggtcatg cgccctgcta
      901 gcacattctc agggcagcag gcccgtcagc cagtgcatta tgttgagccc gaactgcgca
      961 ttgccgggtg ccacgttcat tccgaaccgc tgatcgggcc gcccgctcgg aggtggaaaa
     1021 cggatcaact gggccgacag catccccgcc tcgcccagca cgaccacccg tccccggccg
     1081 tgatcgaacg ccagtccctg cgcgggcaag gccggtgacg agagttccgc cagcgccgcc
     1141 tccgttccgc ctcccccctc tcccgacggc ccaatccggt cggccagcgc ctgcaaggtc
     1201 ggcagatccc gcgactcccg cgccccgacc gtcatcggca gcagcaccgt cgctccgtcc
     1261 ggtccttcaa gagactggcc ggtaaaggcg gttacgctgg tcacccgctc atcagcacct
     1321 cggcccgtga ttatcggatg atcatcgaag gcctcggcgg ggtaggtcag ggtggtcgtc
     1381 aatccgccgg gcggtggtca tcgcaaaggc atagcccttg cccatcctca ccccgaaccg
     1441 cgcggccagc gcctcggccg ccgtaccgaa tggagcgtgg tcggcgatca gcagcagtcg
     1501 tccgccgctc cgcacccagg tctcgacggc ctcgatctcg ctggcggtga agggcgagcc
     1561 gccgtctacc ggcccggcat tggcgatcac cagcacatcg gccccctcca gcccgcccgc
     1621 gtcgaacgcc gcggtcccgc cgcgcacctg atagccgtcg gcccgcaaca gggcggcgaa
     1681 gggggcatag cggccgtcga cggtgtggaa attattgtgc gccccgtcga tcacgatcac
     1741 cgggccttcg gcggcgaagg cgggtcgggc gaccgtcgga tggaagttcg gatcggtgat
     1801 ctgcagcgcc gcgaccggcg cggcgaaggc tatggcgacc aggccgaggg tcagggcgaa
     1861 acggttcatc gaaaaccctc cggtttgtcg cccaccctac cccttcagcc gcgccgcgtg
     1921 aaaggcgacg tgctcgtcta tgaagctggc catgaagaag tac
//
LOCUS       DASVVN010000610         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_23760, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000610 DASVVN010000000
VERSION     DASVVN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23760"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VF686_09920"
     CDS             <1..405
                     /locus_tag="VF686_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270163.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8662170.1"
                     /translation="EPPRLRGYQANNRVTVIIHDLDAVGTTADAVVAAGVNQIDGISF
                     GLKDPTSAENQARQLAVRNLQAKAALYSQALGVQLGGIRSLSEGGGYAPQPPMPMMYA
                     RAEMAQDAGSTPVAAGQLTVRIDITGVYDLVR"
     gene            complement(628..900)
                     /locus_tag="VF686_09925"
     CDS             complement(628..900)
                     /locus_tag="VF686_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662171.1"
                     /translation="MARTIQWVQWALFGLYVVAGAVALMMATPAGLNALGLSGGMVSF
                     VPAMILGLPWSLPTFVMNDGPVTALAVLGICYGLNVGVGLMLLRSR"
     gene            complement(965..1699)
                     /locus_tag="VF686_09930"
     CDS             complement(965..1699)
                     /locus_tag="VF686_09930"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8662172.1"
                     /translation="MKTLACIAAATALALPAAAQDSFANRPTIVVTGNGQASAQPDTF
                     RVDAGVRGRGANQQEALRALAAAQGRLIEAWPKLEGLQRGRVTTGDIALDTLHDPDCE
                     ENARRDEECPILAYFATSALTLEGVPAERAGDAISLASELGATTARLDEYSLSNSDDL
                     RAQANRAAFADAEAQAARLSEVSGRRLVRILRIQDPTAPRSDYDAQEIDEVVVTGSRI
                     RQAVSIAVAPEPVEATARVFVVFEIE"
     gene            complement(1749..2105)
                     /gene="panD"
                     /locus_tag="VF686_09935"
     CDS             complement(1749..2105)
                     /gene="panD"
                     /locus_tag="VF686_09935"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX8662173.1"
                     /translation="MLVTLMKAKLHRATVTQADLDYEGSIAIDADLLDAAGIFPHEQV
                     DVLNITNGARFTTYAIEAPRGSRVIGVNGAAARLVQKNDKVIIVTYGQMPQEEARQWS
                     PTVVLLDDRNDIKRAA"
BASE COUNT          366 a          746 c          754 g          373 t
ORIGIN      
        1 gagccgcccc ggctgcgcgg ctatcaggcg aacaaccggg tgacggtgat catccatgat
       61 ctggacgccg tcggcacgac cgccgacgcc gtggtcgccg cgggcgtcaa tcagatcgac
      121 ggcatctcgt tcggcctcaa ggacccgacc tcggccgaga accaggcccg ccagctggcg
      181 gtgcgcaacc tgcaggccaa ggccgccctg tattcgcagg cgctgggcgt ccagctgggc
      241 gggatccgct cgctgtcgga aggcggcggc tatgcgcccc agccgccgat gccgatgatg
      301 tacgcccgcg ccgaaatggc ccaggacgcg ggctcgaccc ccgtcgccgc cggccagctg
      361 accgtgcgga tcgacatcac cggggtctat gatctggttc gataaggcgc ttgtcatccc
      421 ggacgccgca gcgcggcgat ccgggacgct tgcctacaat gacctgatcc tcccggaagc
      481 tgcttcagca gctgtccggg agacgggtca gacttcagcg cagaggcaac ctgtctcccg
      541 gacaatcgcg ccgcgatttc cgggaggtcg gggttggcgg acgccttgtc ccggatcacc
      601 gtttcgcggc gtccgggatg acaaggctca gcgagacctc agcagcatca gccctacgcc
      661 gacgttcagg ccgtagcaga tgcccagcac cgccagcgcc gtgaccgggc cgtcgttcat
      721 gacgaaagtc ggcaggctcc acggcaggcc aaggatcatg gcgggcacga aggacaccat
      781 ccccccgctc aggccgagcg cgttgagccc tgccggcgtg gccatcatca gggcgacggc
      841 gcccgcaacg acgtagaggc cgaacagggc ccactgaacc cactggatcg tacgcgccat
      901 cgctggtcct cctgaacagc aggggattcc tgaacggagc cggcgtcaag cggcccgtcg
      961 accgtcactc gatctcgaag acgacgaaga cgcgggcggt ggcctccacc ggttcgggcg
     1021 cgacagcgat cgacacagcc tgacggatgc ggcttccggt cacgacaacc tcgtcgattt
     1081 cctgagcatc atagtcagat cggggagccg tggggtcctg aatgcgcaga attcgcacca
     1141 gtcgtcgtcc cgagacttcc gatagccgcg cggcctgggc ttccgcatcg gcaaaggccg
     1201 cgcggttggc ttgcgctcgt agatcgtcgg agttggagag gctgtattcg tccagccgcg
     1261 ccgtcgtggc cccaagctcc gaggccagtg aaatggcgtc gccggcgcgc tcggcgggaa
     1321 ccccttccag cgtcagcgcc gaggtggcga aataggccaa gatgggacat tcctcatctc
     1381 tccgggcgtt ctcttcgcag tccgggtcat gcagcgtgtc caaggcgatg tcgcccgtcg
     1441 tcacgcggcc gcgttgcaga ccttccagct tcggccacgc ctcgataagg cggccttggg
     1501 cggccgctag cgcacgaagc gcttcctgtt ggttcgcgcc ccgtccccgg acgccggcat
     1561 cgacccggaa ggtgtcaggc tgggctgatg cctgcccgtt gcccgtgacg acaatcgttg
     1621 ggcgattggc gaagctgtcc tgagccgcag ccggcagggc cagtgcggtt gcggcggcga
     1681 tgcatgccag cgtcttcatg gtgttctccc cgaggttgaa cccaagggaa ggcttcagcc
     1741 gtgacccgtc aagccgcgcg cttgatgtcg ttgcggtcgt ccagcagcac cacggtcggc
     1801 gaccactggc gggcttcttc ctgcggcatc tggccgtagg tgacgatgat gaccttgtcg
     1861 ttcttctgca ccagacgggc ggcggcgcca ttgacgccga tgacgcgcga accgcgcggg
     1921 gcctcgatgg cgtaggtggt gaaccgcgcg ccattggtga tgttcagcac atcgacctgc
     1981 tcgtgcggga agatgcccgc cgcatccagc agatcggcgt cgatggcgat cgagccttcg
     2041 tagtcgaggt cggcctgggt caccgtggcg cggtgaagct tggccttcat cagggtgacc
     2101 agcatgggga gtgcggtccg gaagtgacgc ggggcgataa gcccccgctc gcagacctct
     2161 catataggcg ttcgcaagga gcccatcaat cgttgtcgtt gcggcgcacc atcggatgct
     2221 gcggcgccgc tcgcgcgtt
//
LOCUS       DASVVN010000611         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_77541, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000611 DASVVN010000000
VERSION     DASVVN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77541"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /gene="thyA"
                     /locus_tag="VF686_09940"
     CDS             <1..390
                     /gene="thyA"
                     /locus_tag="VF686_09940"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX8662174.1"
                     /translation="TALDGPALAPCHCLFQTQVARGRLNLQLYQRSADAFLGVPFNIA
                     SYALLTHMLAQQCGLEPGVFVWTGGDCHLYSNHLDQARLQLTREPRPLPKLAILRRPE
                     AIDRYEYEDFEITGYDAHPHIKAEVAV"
     gene            387..776
                     /locus_tag="VF686_09945"
     CDS             387..776
                     /locus_tag="VF686_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662175.1"
                     /translation="MNPGARPLAVRAAEGCGLIAAVLGGVREAFIWEERTALDGPALA
                     LVFTLAIPLVTALLVLLVTRRRSKAALTVIFLLTALAWLTTAKLGLTDGSVAFTAGAA
                     SLILQSASLLLMLSPPALRRLRAPARG"
     gene            769..1269
                     /locus_tag="VF686_09950"
     CDS             769..1269
                     /locus_tag="VF686_09950"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX8662176.1"
                     /translation="MAEPLSPPRSARPPVVIVVARARNGGIGLHGALPWRIPADLKHF
                     KRVTTGTAMVMGRRTFDSLPGLLPGRRHIVLTRDPEWRAEGAEPASSVEEALELASGQ
                     PVSVIGGAEIIALFLPRADTVELTEVRDDPEADTFMPDFDSADWREVSREDHPGYSFV
                     RLERRA"
     gene            1410..>1697
                     /locus_tag="VF686_09955"
     CDS             1410..>1697
                     /locus_tag="VF686_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662177.1"
                     /translation="MRRIVYGLLALIVIAAGGFLIFGPGIVEGGMNKVVATPLPTTSD
                     RARTLHPRLAIADMHADTLLWQRSLLDPARRGQVDLPRLEAGNVALQIFSSV"
BASE COUNT          250 a          603 c          555 g          289 t
ORIGIN      
        1 acggcgctgg acgggccggc gctggcgccc tgccactgcc tgttccagac ccaggtcgcg
       61 cgcggccggc tcaacctcca gctctatcag agaagcgccg acgccttcct cggagtcccc
      121 ttcaacatcg ccagctacgc cctgctcacc cacatgctgg cccagcaatg cgggctcgaa
      181 ccgggcgtgt tcgtctggac gggcggcgac tgccacctct actccaacca tctcgaccag
      241 gcgcggctgc agctgacgag ggagcccagg ccgctgccga agcttgccat cctcaggcgt
      301 cctgaagcaa tcgaccgcta cgaatatgag gatttcgaga tcaccggcta cgacgcccac
      361 ccgcacatca aggccgaggt ggcggtttga accctggtgc ccggcccctc gccgtccgcg
      421 ccgccgaggg gtgcggcctg atcgcggccg tgctcggggg agtgcgcgaa gccttcatct
      481 gggaagaacg gacggcgctg gacgggccgg cgctggcgct ggttttcaca ttagccatac
      541 ccttggtcac cgccctgctg gtgcttctgg tcactcggcg aaggagcaag gcggcgctga
      601 cggtgatctt cctcctcacc gccctcgcct ggctgaccac cgccaagctc ggccttaccg
      661 acggctccgt cgccttcacc gcgggcgccg catctttgat cctgcagtcg gcgagccttc
      721 tgctgatgct gtcgccgccc gcccttcgcc ggctgcgggc gcctgctcgt ggctgagccc
      781 ctctcgccgc cgcggtcggc caggccgccg gtcgtcatcg tcgtggcccg ggcccgcaac
      841 ggcgggatcg gtctgcacgg cgccctcccc tggcgaattc cggccgatct caagcatttc
      901 aaaagggtga ccacgggcac cgccatggtc atgggccgcc ggacgttcga cagccttccg
      961 ggcctgctcc cggggcgtcg ccacatcgtg ctgacgcgcg atcccgaatg gcgggcggag
     1021 ggagccgagc cggcgtcgtc ggtggaagag gcgctggagc tggcttccgg ccagcccgta
     1081 tccgtgatcg gcggcgcgga gatcatcgcc ctgttcctcc cccgggccga taccgtcgag
     1141 ctgaccgagg tccgtgacga tccggaagcg gacacgttca tgcctgactt cgattcggcc
     1201 gattggcggg aggtttcgag ggaggaccat cccggctaca gcttcgtgag gctggaacgg
     1261 cgagcctagc cttcaccgtc acgctgaact cgtttcaggg tccagtcctc cgcccgcgag
     1321 gcggttcgtc gctggatgct gaaacaagtt cagcatgacg aggggccggt aaggcgggta
     1381 tatccgtcgc gaagccgcaa ggggggaaaa tgcgcaggat cgtctacggg cttctcgccc
     1441 tgatcgtcat cgccgccggc gggttcctca ttttcggtcc cggcatcgtc gagggcggga
     1501 tgaacaaggt ggtggcgact ccgctcccta ccacaagcga ccgcgccagg acgctccacc
     1561 cgcggctcgc catcgccgac atgcatgccg ataccctgct gtggcagcgc agcctgctcg
     1621 acccggccag gcgcggccag gtcgatctgc cgcggctgga ggccggcaac gtcgcgcttc
     1681 aaatcttctc gtcggtg
//
LOCUS       DASVVN010000612         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_49590, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000612 DASVVN010000000
VERSION     DASVVN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49590"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="ribB"
                     /locus_tag="VF686_09960"
     CDS             complement(<1..940)
                     /gene="ribB"
                     /locus_tag="VF686_09960"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX8662178.1"
                     /translation="MDRRDSPISPIEDILEDARQGRPIVLVDAEDRENEGDLIIPAQF
                     ATPEAVNFMARFGRGLICLPITAERARQLRLPPMVAENQTPHRTPFTIAIEAKEGITT
                     GISAHDRARTIAVAIDPTKGPDDIVSPGHIFPLVARDGGVLVRAGHTEAAVDIARLAG
                     LQPAGVLCEIMNDDGTMARLPELTAFAQFHGLKIGTIADLIAYRRRTERQVERVLEQP
                     FESIYGDFRLLVFRNVLDRTEHVALVKGRITPDEPTLVRMHQLDIAADMLGHVENRRD
                     YVPSALTAISDYPGAGVAVFIRDPSPTWLSDRYGGGL"
     gene            complement(973..>1610)
                     /locus_tag="VF686_09965"
     CDS             complement(973..>1610)
                     /locus_tag="VF686_09965"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8662179.1"
                     /translation="SGGRRRPVGALRAGLMFTGIITDVGRVRRVEEKAGGRRLVISTA
                     YDLKDVAVGASICCSGACLSVVEKTASTFTVDVSRETLTRTTLGVWVEGRLVNLERAL
                     KLGDELGGHLVSGHVDGVAEAVRALSDGESLRVQVRAPPPLHRFIASKGSVTLDGVSL
                     TVNEVDGEVFGVNLIPITRTSTTLGDLRGGDRVNLEIDLLARYAARWRETA"
BASE COUNT          226 a          563 c          577 g          244 t
ORIGIN      
        1 ccagcccgcc gccgtagcgg tccgacagcc aggtcgggct cggatcgcgg atgaacaccg
       61 ccacgccggc gccgggataa tcggagatcg ccgtcagcgc ggagggcaca taatcgcgcc
      121 ggttctccac gtggcccagc atgtcggcgg cgatgtcgag ctggtgcatg cgcaccagcg
      181 tcggctcgtc gggcgtgatg cggcccttga ccagcgccac gtgctcggtg cggtccagga
      241 cgttgcggaa caccagcagg cggaagtcgc cgtagatgct ttcgaacggc tgctccagca
      301 cgcgctccac ctggcgctcc gtacggcgcc ggtaggcgat caggtccgcg atggtgccga
      361 tcttcagccc gtggaactgg gcgaaggcgg tcagctccgg cagccgggcc atggtgccgt
      421 cgtcgttcat gatctcgcac agcacgccgg ccggctgcag cccggccagc cgcgcgatgt
      481 cgaccgccgc ctcggtgtgg ccggcccgga cgaggacgcc gccgtcgcgc gcgaccagcg
      541 ggaagatgtg gcccgggctg acgatgtcgt cgggcccctt ggtcgggtcg atggccaccg
      601 cgatggttcg cgcgcggtca tgggcggaga tgccggtggt gatgccttcc ttggcctcga
      661 tggcgatggt gaagggcgtg cggtgcggcg tctggttctc ggccaccatg ggcggcaggc
      721 gcagctggcg cgcgcgctcg gcggtgatcg gcaggcagat caggccgcgc ccgaaccggg
      781 ccatgaagtt caccgcctcg ggagtggcga actgcgccgg gatgatcagg tcgccctcgt
      841 tctcgcggtc ctcggcatcg accagcacga tcggccggcc ctggcgggcg tcctccagga
      901 tgtcctcgat gggcgagatg gggctgtcgc ggcggtccat ttcggtcgat gggtactcgt
      961 aggtctttga cttcacgccg tctctctcca gcgggcggcg tagcgcgcca acaggtcgat
     1021 ctccaggttg acccggtccc cgccgcgcag gtcgccgagc gtggtcgagg tgcgggtgat
     1081 ggggatcaga ttcaccccga acacctcccc gtccacttcg ttcaccgtca gcgagacccc
     1141 gtccagggtg accgacccct tggaggcgat gaaccggtgc agcggcggcg gcgcgcgcac
     1201 ctgcacccgc agcgactcgc cgtccgagag ggcgcggacc gcctccgcca ccccatccac
     1261 gtggccggag accaggtggc cgccgagctc gtcgccgagc ttcagcgccc gctccaggtt
     1321 gaccagccgc ccctcgaccc agacgccgag ggtggtgcgg gtcagcgtct cgcgggagac
     1381 gtccacggtg aaggtcgagg cggtcttctc gaccaccgac aggcaggcgc ccgagcagca
     1441 gatcgacgcc ccgacggcga cgtccttgag gtcgtaggcg gtggagatga ccagccgccg
     1501 gccgcccgcc ttctcctcca cgcgccggac gcggccgacg tcggtgatga tgccggtgaa
     1561 catcaggccc gctcgtagcg ctcccacagg tcgtcgccga cctcctgaac
//
LOCUS       DASVVN010000613         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_77645, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000613 DASVVN010000000
VERSION     DASVVN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77645"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF686_09970"
     CDS             complement(<1..641)
                     /locus_tag="VF686_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662180.1"
                     /translation="MAEEDAGGVGHGEAPEWTVTLSRRAAGFKRLVEIIDRTRDAADD
                     AGMKAPIALPTSSVARNALVLIAVVAAGAAVYWLRGILTPLAMAIFLMIMVDGVKRTI
                     EERTPIPGRWAGAAALTVVVLGFLASIAIIVNGAAGFFDQATGITTRIGPRIDQILID
                     GGRLFGVQAAPTAAELLAGLDVRSYVTAAAMQAQGILSGAFFVMVYLAFLLAS"
     gene            609..2186
                     /locus_tag="VF686_09975"
     CDS             609..2186
                     /locus_tag="VF686_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX8662181.1"
                     /translation="MTDAPGIFLGQSLDDGASLPETLLLNRANRHGVVAGATGTGKTV
                     TLQIMAQGFSDAGVPVFCADVKGDLSGICVPGSPNEKLLARSASMGLTLQPKAAPVVF
                     WDLYGLKGHPIRTTVSEIGPVLLARMLNLNEVQEGVLSVLFHVADTEKLLLLDLDDLR
                     AMLAYVGENADRIGREVGNVAPASIASIQRAILQLEQQGGKAFFGEPALRLEDMMRTG
                     LDGRGQVNVLDATKLMASPRLYAAFLLWLLSELFEQLPEVGDPDKPRLVFFFDEAHLL
                     FNDTPDALREKVEQVVRLIRSKGVGVYFVTQNPADIPDSVLAQLGNRIQHALRAYTPA
                     EQRGLKAASESFRANPAFGTAEAIQGLGVGEALVSTLDEKGAPQVVARTAIRPPDSRL
                     GPATDAERAAVMAASPVRGLYETVVDRESAEEILAARRGAQDQAAADAKLAGARAKAD
                     AAAAKDAEKAAVVREKEQAREQARYDREAARPRAAPRRSTRETPIEAATKSVLRTAGS
                     TLTRELLRGLLGGLKRR"
     gene            2189..>2406
                     /locus_tag="VF686_09980"
     CDS             2189..>2406
                     /locus_tag="VF686_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662182.1"
                     /translation="MNDGIALALQFPGVFFSTLLRPLGFSGVFLITAVATACLIGGIA
                     FGILRRKAVLLSFLAPAALSELLLVLASL"
BASE COUNT          393 a          884 c          767 g          362 t
ORIGIN      
        1 tgggacgcca gcaggaaggc cagatagacc atgacgaaga acgcgccgga caggattccc
       61 tgcgcctgca tggccgccgc cgtcacatag ctgcgcacgt ccagaccggc cagcagttcg
      121 gccgccgtgg gcgccgcctg cacgccgaac agccgcccgc cgtcgatcag tatctggtcg
      181 atgcgcggcc cgatccgggt cgtgatgccc gtcgcctgat cgaagaagcc cgccgcgccg
      241 ttcacgatga tggcgatcga ggcgaggaag cccaacacca ccaccgtcaa ggccgccgcc
      301 cccgcccagc gacccggaat gggcgtgcgc tcctcaatgg tccgcttcac cccgtcgacc
      361 atgatcatca ggaagatggc catggccaac ggcgtcagta tgccccgcag ccagtagacc
      421 gccgccccgg ccgccaccac ggcgatcaga accagcgcgt tgcgcgcgac gctcgaggtc
      481 ggcagggcga tgggagcttt catgcccgca tcgtcggcgg cgtcgcgggt tcggtcaatg
      541 atctcgacaa ggcgcttgaa gcccgccgcc cgccgcgaca gggtgacggt ccattcagga
      601 gcctccccat gaccgacgcc cccggcatct tcctcggcca atcgctggat gacggcgcca
      661 gcctgcccga gaccctgctg ctgaaccgcg ccaaccggca cggcgtcgtc gccggcgcga
      721 ccggcacggg caagacggtc accttgcaga tcatggccca gggcttttcg gacgccggcg
      781 tcccggtctt ctgcgccgat gtgaagggcg acctgtcagg catctgcgtg cccggctcgc
      841 ccaatgagaa gcttctggcc cgctccgcct ccatggggct gacgcttcag ccaaaggccg
      901 cgcccgtcgt cttctgggac ctgtacggcc tcaagggcca tccgatccgc accacggtca
      961 gcgagatcgg cccggtgctg ctggcgcgga tgctgaacct caacgaggtg caggaaggcg
     1021 tcctgtcggt gctcttccac gtcgccgata cggaaaagct gctgctgctc gacctcgacg
     1081 acctgcgcgc catgctggcc tatgttggcg agaacgcgga ccgcatcggc cgcgaggtcg
     1141 gcaatgtcgc ccccgcctcc atcgcctcga tccagcgcgc catccttcag ctggagcagc
     1201 agggcggcaa ggccttcttc ggcgagccgg ccctacggct ggaggacatg atgcgcaccg
     1261 gcctcgacgg ccgcggtcag gtcaatgtgc tcgacgccac gaagctgatg gccagcccgc
     1321 gcctctacgc cgccttcctg ctgtggctgc tgtcggaact gttcgaacag ctgcccgagg
     1381 tcggcgaccc ggacaaaccg cgcctcgtct tcttcttcga cgaggcccat ctgctgttca
     1441 acgacacgcc ggatgcgctg cgcgaaaagg tcgaacaggt cgtgcgtctg atccgctcca
     1501 agggcgtggg cgtctatttc gtcacccaga acccggccga cattcccgac agcgttctgg
     1561 cccagctggg caaccgcatc cagcacgcgc tgcgcgccta cacgcccgcc gaacagcgcg
     1621 gcctgaaggc agcctccgaa agcttccgcg ccaacccggc cttcggcacc gccgaggcca
     1681 tccagggcct gggcgtcggc gaggccctgg tctcgacgct ggatgagaaa ggcgcgcccc
     1741 aggtcgtcgc ccgcaccgcc atccgcccgc cggacagccg actgggcccc gccaccgacg
     1801 cggaacgcgc cgcagtcatg gcggccagcc ccgtgcgcgg cctctacgag accgtggtcg
     1861 accgcgaatc cgccgaggaa atcctcgccg cccgccgggg cgcacaggat caggccgccg
     1921 ccgacgccaa actggccggg gcccgcgcga aagccgatgc cgccgccgca aaggacgctg
     1981 aaaaggccgc cgttgtgcgc gagaaggagc aggcccgcga acaggcccgg tacgaccggg
     2041 aagcagcccg tccccgcgcc gcgccgcgcc gctcgacccg cgaaaccccg atcgaagccg
     2101 cgaccaagtc ggtgctgagg acggcgggct ctactctgac gcgggagctg ctgcgcggct
     2161 tactaggcgg gctgaagcgg cggtaatcat gaacgacggt atcgcactgg ctctgcagtt
     2221 tccgggcgta ttcttctcga ccctgcttcg gccccttggc tttagcgggg ttttcctgat
     2281 caccgccgta gcgacggcat gtctcatcgg tggaattgcc ttcggtattc tcaggcgaaa
     2341 agcggtcctt ctctcttttc tggccccggc ggccctgagc gagctgcttc tcgtgctcgc
     2401 gagcct
//
LOCUS       DASVVN010000614         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_313394,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000614 DASVVN010000000
VERSION     DASVVN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313394"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF686_09985"
     CDS             <1..205
                     /locus_tag="VF686_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8662183.1"
                     /translation="VGATATLELGPRDAEALALAKSEGELSLVLRSYADTGGPSGRVA
                     PPTRQSTPVRVFRGGAPEVVVVQ"
     gene            214..1641
                     /locus_tag="VF686_09990"
     CDS             214..1641
                     /locus_tag="VF686_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX8662184.1"
                     /translation="MTRLAVLALAGLTLAAAAPVMPDAAQAQSRAAVPMGAGAQLINL
                     PRGSSMAIDLPSDARDVIVPNPVVAEAMLHSPRRITIIGLQPGETDAVVLDAAGRTIL
                     SLRVRVDAGVSALQDTLSRVMPGSQVHAEAVNDSIILTGPVNSPGEADRASQVARAFV
                     SAPEKVINMMTIAGSDQVMVKARVIEVQRTAIKQLGLDVDAVLNNVGDGLSFAQSATF
                     GVSGSQLGGGILNYLDQSGDGNSLDTSIRAFERAGLVRILAEPNLTSVNGENAEFLAG
                     GEFPVPTGRDVDSTTGQVTIGIEFKPFGVRLAFRPIVLSQGRISLQLSTEVSELTQEG
                     AFTLGGTADDALVIPALKVRRASSTVELPSGGSMMIAGLLREDTRQNIDQLPGIGNLP
                     VLGALFRSRDYLSGETELVIIIEAYIVSPTSPGRLQTPADGLIIANDAQTLLFGQLDQ
                     QYRPAGGTPAGTNAAWTGPVGYVIE"
     gene            1653..>2295
                     /locus_tag="VF686_09995"
     CDS             1653..>2295
                     /locus_tag="VF686_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEX8662185.1"
                     /translation="MRATASAVFVLVLGLGAAGCSTMAGSDPLAPVTPLSRYSLQVEP
                     GTDRIALAVHDQGLSANQRDAIGQLAARYGAAGYGALTVEAPAGDDPAAVQHAYAVRA
                     ALQAAGVPGDRIQIAAYAAPDPRAPVLAGFETVRAVVPNCALRAGDLGPRYSNQSSNG
                     FGCSVTANMAAQIADPRDIMAARAMTPADSGRAAVVFDNYRKGDTSSTPQEPLV"
BASE COUNT          351 a          877 c          726 g          341 t
ORIGIN      
        1 ggtcggcgca accgccaccc ttgaactggg cccccgcgac gccgaagctc tcgcactggc
       61 caagtcggaa ggcgaactga gcctggtcct gcggtcctac gccgacaccg gcggcccttc
      121 cggccgggtc gccccgccga cgcgacaatc aacccccgtc cgcgtattcc gcggcggcgc
      181 gcccgaagtg gtggtggtcc aatgaaacgc ccgatgacgc gtcttgccgt cctcgccctg
      241 gccggcctca ccctggccgc cgcggcgccc gtcatgcctg acgccgccca agcccagtca
      301 cgcgccgccg tgcccatggg cgccggcgcg cagctgatca acctgccgcg cggctcgtcc
      361 atggccatcg atctgccgtc ggacgcccgc gacgtgatcg tacccaatcc cgtggtcgcc
      421 gaggccatgc tgcactcgcc gcgccgcatc accatcatcg gcctgcaacc cggcgagacc
      481 gacgccgtgg tcctggacgc cgccggccgg acgatcctgt ccctgcgcgt ccgcgtcgac
      541 gcgggcgtgt cggccctgca ggacacgttg agccgcgtca tgccgggctc ccaggttcac
      601 gccgaagcgg tcaacgacag catcatcctg accggacccg tgaacagccc cggcgaagcc
      661 gaccgcgcct cccaggtcgc gcgcgccttc gtctcggcgc ccgagaaggt catcaacatg
      721 atgaccatcg ccggttccga ccaggtcatg gtcaaggccc gggtgatcga ggtgcagcgc
      781 acggcgatca agcagctggg cctggacgtc gacgccgtgc tgaacaacgt cggcgacggt
      841 ctgtccttcg cccaatcagc aaccttcggc gtatctggca gccagttggg cggcggcatc
      901 ctcaactacc tggaccagag cggcgacggc aacagcctgg acaccagcat ccgcgccttc
      961 gaacgcgcgg gactggtccg catcctggcc gagccgaacc ttacctccgt caacggcgag
     1021 aacgccgaat tcctcgcggg cggcgaattc cccgttccca ccggccgcga cgtggactcc
     1081 accaccggtc aggtcacgat cgggatcgag ttcaagccgt tcggcgtgcg cctggcgttc
     1141 cggcccatcg tcctgtcgca gggtcgtatc tcgctgcaac tttcgaccga agtgtccgaa
     1201 ctgacccagg aaggcgcgtt cacgctcggc ggcaccgccg acgacgccct ggtcattccc
     1261 gctctgaagg tgcgccgcgc ctcctccact gtcgagctgc cctcaggcgg ctccatgatg
     1321 atcgccggcc tgctgcgcga agacacccgc caaaacatcg accagctgcc gggcatcggc
     1381 aatctgccgg tgctgggcgc gctgttccgg tcgcgcgact atctgtcggg cgagaccgaa
     1441 ctggtcatca tcatcgaagc ctacatcgtc tcccccacct cgcccgggcg gctgcagacg
     1501 cccgccgacg gcctgatcat cgccaacgat gcccagaccc tgctgttcgg tcagctggac
     1561 cagcaatatc ggcccgccgg tgggacaccc gccgggacca acgccgcctg gaccggcccc
     1621 gtgggctatg tgatcgaatg aggattccgg ccatgcgcgc caccgcttcc gcagtattcg
     1681 tcctggttct cggccttgga gccgcaggct gctccaccat ggcgggcagc gatccgctgg
     1741 cgcccgtcac gcccctgtcg cggtacagcc tgcaggtaga acccggcacg gaccggatcg
     1801 cgctcgcggt gcacgaccag ggcctttccg ccaaccaaag ggatgcgatc ggccagctgg
     1861 ctgctcgtta cggcgcggcc ggatacggcg ccctcacggt cgaggcgccg gcgggcgacg
     1921 acccggccgc cgtccagcac gcctatgccg tccgggcggc cctgcaggcg gccggcgtgc
     1981 cgggggaccg gatccagatc gccgcctacg ccgcgcccga tccccgcgcg ccggtgctgg
     2041 ccgggttcga gaccgtgcgg gccgtcgtcc ccaactgcgc tctgcgcgcg ggcgacctgg
     2101 ggccacgcta ttcgaaccag agttcgaacg gcttcggctg ttcggtcacc gccaacatgg
     2161 ccgcccagat cgccgatcca cgggatatca tggccgcccg ggccatgacg cccgccgatt
     2221 ccggccgagc cgcggtggtg ttcgataact accgcaaggg ggacacaagt tcgacgccgc
     2281 aggagcccct cgtga
//
LOCUS       DASVVN010000615         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_470747,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000615 DASVVN010000000
VERSION     DASVVN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470747"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VF686_10000"
     CDS             complement(<1..307)
                     /locus_tag="VF686_10000"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEX8662186.1"
                     /translation="MAVCPAPSTGDAFLSGLLRHIDCQGETLGAAGYQLLADPSSPLA
                     LVLTALLTIFVALFGLRMMLGETPTLRDGVMAVAKIGVVLTIATSWPAWRTVVYDVVV
                     "
     gene            complement(307..>2042)
                     /locus_tag="VF686_10005"
     CDS             complement(307..>2042)
                     /locus_tag="VF686_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEX8662187.1"
                     /translation="LLPHQDVGAYLPYRRVSFGQEAVELSAAGHVPRSFLGLISIKDY
                     PAFSAPGMFDELLRLPFELTITQSFAFVERQAALERMNLALRRMRSAEDEAISLRSEL
                     AQAKDDVAAGRAGYGEHHMTIAVRGAAPAAVDAAVAEVTASLADLGIVAVREDIAVEP
                     AFWAQFPANFKYIARRGLVSTNNFAGLASAHNFPLGAATGSHWGDAVTLLETTAAGPY
                     FFNFHQGDLGNFTVIGPSGSGKTVVLNFLLAQARKLNPRIIFFDKDRGAELFIRAIGG
                     RYDVLRPGQASGLNPLQLEDTPANRGFLVDWLSQLAGGADVNDLARIRDAIDANFDQP
                     AAHRRLRHLVELFRGDQRPHAEDLWSRLRPWWGKGELAWLFDNPEDLTDLSIETVGFD
                     MTVLLDDPAVRTPAMMYLFHRVEERLDGSPAIIVVDEGWKALDDDVFVRRIKDWEKTI
                     RKRNGIVGFATQSAQDALESRIASAIIEQAATQIFMVNTKARAEDYVEGFGLTPHELD
                     LIRTLPDSAHCFLIKRGGESVVARLDLTGETDVLIILSGRERTVRLLDEIRASVGDDP
                     ADWMPPLLERA"
BASE COUNT          364 a          684 c          679 g          315 t
ORIGIN      
        1 caacgacgac gtcgtagacg acggtgcgcc aggccggcca gctggtcgcg atcgtcagca
       61 cgacgccgat cttggccacc gccatcaccc cgtcgcgcag ggtcggcgtc tcgcccagca
      121 tcatgcgcag accaaacaga gcgacgaaga tcgtcaacag ggcggtaagc accagggcca
      181 gcggcgaact gggatcggcc agcagctgat agccggcggc gccgagcgtc tccccctgac
      241 agtcgatgtg gcgcagcaag cccgacagga aggcgtcgcc ggtggatggc gccggacaga
      301 ccgccatcac gcgcgctcca gcaggggcgg catccagtcc gccggatcat cgccgaccga
      361 ggcccgtatc tcgtcgagca accggacggt ccgctcgcga ccggaaagga tgatcaggac
      421 atccgtctcg cccgtcaggt caagccgggc gaccacgctc tcccctcccc gtttgatgag
      481 aaaacagtgc gcactgtccg gcaaggtgcg gatcaggtcg agctcgtgcg gcgtgagccc
      541 gaagccctcg acatagtctt cggcccgcgc cttggtgttg accatgaaga tctgggtcgc
      601 ggcctgttcg atgatcgcgc tggcgatgcg gctctccagc gcgtcctgcg cgctctgggt
      661 cgcgaagccg acgatcccgt tgcgcttgcg gatggtcttt tcccagtcct tgatgcgacg
      721 gacgaagaca tcatcgtcca gcgccttcca gccctcgtcg acgacgataa tcgccggaga
      781 cccgtccagc cgctcctcca cgcgatggaa gagatacatc atcgccgggg tgcggacggc
      841 gggatcatcg agcaacaccg tcatgtcaaa gccgacggtc tcgatcgaca ggtccgtcag
      901 gtcttccggg ttgtcgaaca gccaggccag ttcccccttg ccccaccacg gacgtagccg
      961 cgaccacagg tcctcggcgt gaggacgctg gtcgccgcgg aacagttcga ccagatgcct
     1021 cagccggcga tgcgcggccg gctggtcgaa attggcgtcg atcgcatcgc ggatcctcgc
     1081 cagatcgttg acgtcggcgc caccggccag ctggctcagc cagtcgacga ggaagccgcg
     1141 gttggccggc gtgtcttcca gctgcagcgg gttgagcccc gaggcctggc caggacgaag
     1201 cacgtcgtag cggccgccta tggcgcggat gaacagttcc gcgccgcgat ccttgtcgaa
     1261 gaagatgatg cgcgggttca gcttgcgcgc ttgggcgagc aggaagttga gcaccaccgt
     1321 cttacccgag ccggacgggc cgatgacggt gaaattgccc aggtcacctt ggtggaagtt
     1381 gaagaaatac ggccccgcgg ccgtggtctc cagcagggtg acagcgtccc cccagtgaga
     1441 gcccgtcgcc gcccccagcg ggaagttgtg ggcgctggca agacccgcga aattgttggt
     1501 cgagaccaag ccgcgtcggg ctatatattt gaagttcgct gggaactggg cccagaaggc
     1561 cggttccacc gcgatgtcct cgcggacggc gacaatgccc aagtccgcga ggctggccgt
     1621 cacctcggcc acggcggcgt cgaccgcggc gggcgcagcc ccgcgcacgg cgatggtcat
     1681 atgatgctcg ccgtagcccg cccggccggc cgcgacgtcg tccttggcct gggccagttc
     1741 gctgcggagg gagatggcct catcctccgc cgagcgcata cgccgcagcg ccaggttcat
     1801 acgctccagc gcggcctgtc gctccacgaa ggcgaacgac tgggtgatcg tgagctcgaa
     1861 cggcaggcgc agaagttcgt cgaacatgcc cggcgcgctg aaggccgggt agtctttgat
     1921 cgatatcagg ccgagaaaac tgcgcgggac gtggcccgcc gccgagagct ccaccgcttc
     1981 ctgcccgaaa ctgacgcggc ggtagggcag atacgccccc acgtcctgat gcggcagcag
     2041 ga
//
LOCUS       DASVVN010000616         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_419152,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000616 DASVVN010000000
VERSION     DASVVN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419152"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VF686_10010"
     CDS             <1..551
                     /locus_tag="VF686_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662188.1"
                     /translation="GSGKTGQAALERLARSDNADIATRAKNVLAANRHQPLRPLAPER
                     AAMARDIRINTYPAGATLPASFTGQYARLAEGLDHCKDEHACTARLIDLDGDGQSEVL
                     VGGEMSASAFKRETDGRWMEWVMYRAPECDNDDSFNVAEAVGSPRLTTAPPIFPDLVI
                     GGVRLRRQEGELECPAPPDPAP"
     gene            665..1828
                     /locus_tag="VF686_10015"
     CDS             665..1828
                     /locus_tag="VF686_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8662189.1"
                     /translation="MTQDGPQAELLRRAAEALARRDPQSALGLLDQADRLGVTHNGTL
                     NRALALRLIGDFNGALTVLDDALAMQPYDFTALLGKAAMLEQIGRGHAAVDIYRNALK
                     IAPPRDRCPPPIAAQMDHAAGVVRRHAEALRDHMKSEVAALRESVDPAALDRFDEGLE
                     IYAGLKTAPKQEPLLLDYPRLPAIPFYDRALFPWLETLEAATPVIQAELEALLASSMD
                     EFAPYIAYPKGAPVNQWAELNHSHKWSSLWLWRDGERQDAVCERCPQTAALLDSLPMC
                     RQEGFAPTAVFSALQPHTHIPAHTGSSNVRLLTHLPLILPGPARFRVGNTVRDWKMGQ
                     AWVFDDTIEHEAWNDADQMRVILIFDIWNPWLTDGEKALITAMMQAQRSFTEG"
     gene            complement(1825..2787)
                     /locus_tag="VF686_10020"
     CDS             complement(1825..2787)
                     /locus_tag="VF686_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662190.1"
                     /translation="MADHPYLPATLVDPVPSNAEVYAADPAAPLTLIYIHGIKNKPPV
                     EVIKCQWDMAIFRKETAASRFAYWVNRKRYPRPVAATCADRDVTDGLDPAARNPQAEA
                     ELETEIRNLGATPEEQAFLEATAGEMLSRAPSSPKAASGAARDWLARYLARSLLPDAY
                     DFLYDKKRHAEICRIADDAINASGGRSIVIGHSLGSVIAYEVLRKRPAGAPEVPLFLT
                     LGSPLGLSTVMAVLRRWSGADGKKLPCPPGVMKWINVAARYDVIAVDADLADEFDGAE
                     DYLITNGDDDPHSATGYLVSGIVRSSIHLLTGFPADAGRPQATS"
     gene            2990..4450
                     /locus_tag="VF686_10025"
     CDS             2990..4450
                     /locus_tag="VF686_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8662191.1"
                     /translation="MAKTVLVVDDDPTQRRLAQAVLEREGYAVVHAESGGEAIDRLTK
                     GGGADVVLLDMVMPGMSGMEALAEIRTAGVTTPVIVLTASGGVDAVVKAMQAGAQDFF
                     VKPVSAERLLVGVSNALQMTRLTAEVGRLKKHVAGRTSFDDLVGQSAPMRMVKSLGTR
                     AAKSSIPVLITGESGVGKEVIARALHGASDRSGKPFVAVNCGALPSNLIESILFGHEK
                     GAFTGAHDKHLGKFMEANGGTLFLDEIGELPLDMQVKLLRALQEGEIDPVGAKRSVKV
                     DVRIVSATNRDPAQQVREGAFREDLFYRLNVFPIEAPSLRERREDIPALVDHFVARFN
                     VEEGKRIAGCSGETLALLCAFDWPGNVRQLENAVYRAIVLADAPFLQPHDFPAISGRA
                     TPLAADQGPMDQAVTAQALADIDLPPLPDSPIRILDERGHLRTLEEIERDLIQHAIEV
                     YAGHMSEIARRLGIGRSTLYRKVREQGLEGSLREVG"
     gene            4656..>5379
                     /locus_tag="VF686_10030"
     CDS             4656..>5379
                     /locus_tag="VF686_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8662192.1"
                     /translation="MLLVTTALSGLMAGTIQTAPPQTAPQTPPASAPVASAPVVAGDD
                     VADLGTVVATGARARGSVDSDIPPDVTLTAEQIQAYGASDIAELLTYLEPLTRSSRGR
                     GDGQPVLLVNGRRISGFQEIQGIPTEAIERTDILPEEVALQYGYRADQRVVNFVLKSN
                     FRSLVGQLSGRAPTQGGRSIGEIEGNVLRIAGATRWSVDAEYERSTPLYESERDIERD
                     PSAGEFGDPDLRGAYRTLLSESE"
BASE COUNT          919 a         1968 c         1640 g          852 t
ORIGIN      
        1 acgggagcgg caagacggga caggcggctc tggagcgttt ggcgcgctcg gacaacgccg
       61 acatcgccac ccgtgcaaag aacgtcctgg cggcgaaccg tcaccagccc ctgcgccccc
      121 tcgccccgga gagggctgcg atggcccgcg acattcgcat caacacctat ccggcaggcg
      181 cgacgctccc cgcctccttc accggccagt acgcccgcct cgccgaaggc ctcgaccatt
      241 gcaaggacga acacgcctgc accgcacgcc tgatcgacct tgacggcgac ggtcagtccg
      301 aagtgctggt cggcggcgaa atgtccgcat cagccttcaa acgggaaacg gacggtcgct
      361 ggatggaatg ggtcatgtat cgggcgcccg agtgcgacaa cgacgactcc ttcaatgtcg
      421 ccgaggctgt cggctccccc cgactcacga ccgccccgcc catcttcccc gacctggtca
      481 ttggcggcgt caggctccgc cgtcaggaag gcgaactcga atgccccgcc ccgccggatc
      541 ccgccccctg aacccccgcc gccacagccc cacttaatcc gcgaccgtac gtaaaccgct
      601 cttgacccgg acggtccggc taacattaca gtcagttcat gatatcggga tcgggggacc
      661 ccgcatgaca caggacggac cacaggccga gttgctgcgc cgcgccgcgg aagcgctcgc
      721 gcgccgcgat cctcaatcgg ctctcggtct gctggatcag gccgaccgac tgggcgtcac
      781 ccacaacggc acgctcaacc gggcgctggc gctacgcctg atcggcgatt tcaacggcgc
      841 cctgacggtg ctggacgacg ccctggcgat gcagccgtat gatttcaccg ccctgctggg
      901 caaggcggcc atgctggagc agatcggcag gggccacgcc gccgtcgaca tctatcgcaa
      961 cgccctgaag atcgccccgc cgcgcgaccg ctgcccgcct ccgatcgcgg cccagatgga
     1021 tcacgccgcc ggcgtcgtgc gccgtcacgc cgaggccctg cgcgaccata tgaagtccga
     1081 agtcgccgcc ctgcgagagt cggtcgatcc ggcggcgctg gaccgcttcg acgaggggct
     1141 ggaaatctac gccggtctga agacggcgcc gaagcaggaa cccctgctgc tcgactatcc
     1201 ccgtctgccg gccatcccct tctacgaccg cgccctgttc ccctggctgg aaacgctcga
     1261 ggccgccacc ccggtcatcc aggccgagct tgaggccctg ctggcctcgt ccatggacga
     1321 attcgccccc tatatcgcct accccaaggg cgccccggtc aatcagtggg ccgagctgaa
     1381 ccattcgcac aaatggtcct ccctctggct gtggcgcgac ggcgaacgac aggacgccgt
     1441 ctgcgaacgc tgcccgcaga ccgccgccct gctcgattcc ctgcccatgt gccgtcagga
     1501 ggggttcgct cccaccgcgg tcttctcggc ccttcagccc cacacccaca tcccggccca
     1561 caccggctca tccaacgtcc ggctgctcac ccacctgccg ctgatcctgc ccggccccgc
     1621 ccggttccgc gtcggcaaca cggtgcgcga ctggaaaatg ggtcaggcct gggtcttcga
     1681 cgacaccatc gaacacgagg cctggaacga cgccgaccag atgcgggtca tcctgatctt
     1741 cgacatctgg aacccctggc tgacggacgg cgaaaaagcc ctgatcaccg ccatgatgca
     1801 ggcccagcgc agcttcacgg agggctagga cgtggcctgc ggccgtccgg cgtcggcggg
     1861 gaatcccgtc agcaggtgga tcgaagatct gacgattccg ctcaccagat agccggtcgc
     1921 cgaatggggg tcatcatccc cattggtgat gagatagtct tccgcgccgt caaactcatc
     1981 cgccagatcg gcatcgaccg cgatgacgtc atatcgtgcg gcgacattga tccacttcat
     2041 cacgcccggc ggacacggga gtttttttcc atccgccccg ctccacctcc tgagcaccgc
     2101 catcacagtg ctcagtccca gcggcgagcc gagcgtcaga aacaacggga cctccggggc
     2161 gccggccggc cgtttgcgaa ggacctcgta cgcgatgacg cttcccaggc tgtggccgat
     2221 cacgattgat ctgcctccgg acgcgttgat cgcgtcgtca gcgatccggc aaatctcggc
     2281 gtggcgtttc ttgtcgtaga ggaagtcgta tgcgtccggc aggagggatc gcgccagata
     2341 tctggccagc caatcccgtg cggcgcccga cgcagccttc ggactggaag gcgccctgga
     2401 aagcatctcg cccgcggtcg cttccagaaa cgcctgttct tcgggcgttg cgccgaggtt
     2461 tctgatctct gtttcgagct ccgcctccgc ttgcggattg cgggcggccg gatccagccc
     2521 gtcggtcacg tcccgatcgg cgcaggtcgc cgccaccggt cgcgggtatc gctttcgatt
     2581 gacccaatag gcaaagcggc tggcggccgt ctccttgcgg aaaatcgcca tgtcccactg
     2641 gcatttgatc acctcgacgg gcggcttgtt cttgatgccg tggatgtaga tcaaggtcag
     2701 cggcgcggcc ggatcggcgg cgtaaacctc ggcgttggaa ggaacgggat cgaccagtgt
     2761 ggccggcagg taaggatggt cagccacggc gcgtctccca cggctctgct aaaggttgca
     2821 ggcagacaca acttatcgaa aaaacaccga tacgcaacga acgttaaccc tgcgctcacc
     2881 accccgcaca acccgatctt caccttgagc ggggtagaaa cgtcccggaa tgggccagtc
     2941 aagctttcgg cgggacaggc ctgacgtgat gatgaagagg cgccttccca tggccaaaac
     3001 cgttctggtg gtcgacgacg atccgaccca gcgccgcctc gcacaggccg tgctggagcg
     3061 cgaaggctat gccgtcgtcc acgcggaatc gggcggcgag gccatcgatc gcctgacgaa
     3121 aggcggcggc gccgacgtcg tcctgctgga catggtcatg cccggcatga gcggcatgga
     3181 ggccctggcc gagatccgca ccgccggggt cacgaccccc gtcatcgttc ttaccgcctc
     3241 aggcggggtc gacgccgtgg tcaaggccat gcaggccggg gcccaggact tcttcgtcaa
     3301 gcccgtctcg gccgaacgcc tgctggtcgg ggtctccaac gccctgcaga tgacgcgcct
     3361 gaccgccgag gtgggccgcc tcaagaagca cgtcgccggc cgtacctcct tcgacgacct
     3421 cgttggccag tcggcgccca tgcgtatggt caagtcgctg gggacgcgcg ccgccaaatc
     3481 ctcgatcccg gtgctcatca cgggcgaaag cggcgtcggc aaggaagtca tcgcccgcgc
     3541 cctgcacggc gcgtcggacc gctccggcaa gcctttcgtg gcggtcaact gcggcgccct
     3601 gccctccaac ctcatcgagt ccatcctgtt cgggcacgag aagggcgcct tcaccggggc
     3661 ccacgacaag cacctcggca aattcatgga ggccaacggc ggcaccctgt tcctcgacga
     3721 gatcggcgaa ctgccgctgg acatgcaggt caagctgctg cgcgccctcc aggaaggcga
     3781 gatcgacccc gtcggcgcca aacggtcggt caaggtcgac gtccgcatcg tctcggccac
     3841 caatcgggat ccggcgcaac aggtgcgcga aggcgccttc cgcgaggatt tgttctatcg
     3901 cctcaacgtc ttcccgatcg aggctccgtc cctgcgcgaa cgccgcgagg acattccggc
     3961 cctcgttgac catttcgtcg cccgcttcaa tgtcgaggag ggcaagcgca tcgccggctg
     4021 ttcgggcgag accctggccc tgctgtgcgc cttcgactgg cccggcaacg tccgtcagct
     4081 cgagaacgcc gtctatcgcg ccatcgtcct ggccgatgca cccttcctcc agccgcacga
     4141 cttccccgcc atctccggcc gggcgacgcc gctggcggcc gatcaagggc caatggatca
     4201 ggccgtgacc gcgcaagcgc tcgccgacat cgacctgccg ccgctgccgg attcgccgat
     4261 ccgcatcctc gacgagcgcg gccacctgcg caccctggaa gagatcgagc gcgacctgat
     4321 ccagcacgcc atcgaggtct acgccggcca catgtccgag atcgcccgcc gcctcggcat
     4381 cggccgctcg actctgtacc gcaaggtgcg cgaacagggt ctggaaggca gcctcaggga
     4441 agtcggctag gcaaagccgg ctcccgtgtg acgcatttcg gcaacgtccc ggcaatgcct
     4501 gcagtctgac caaaagttca ttggccagcg gccgcgcgaa ccccgaagac gcatgggctg
     4561 tgcgcacccg caaccttcgg tttcctcgtt gaaacgcgac ggaaacatga gcgggcgacg
     4621 ttgcagcttc gtcctcctcc cccaaggtcc gctgcatgct tctcgtcacc accgccctgt
     4681 ccggactcat ggccgggacg atccagaccg cgccgcctca aacggctccg caaacgccgc
     4741 cggcctctgc gcccgtcgcg tccgcgccgg tcgtcgccgg cgatgacgtc gcggatctcg
     4801 gcaccgtggt ggccacgggc gcccgcgcgc gcggcagcgt cgactccgac atcccgcccg
     4861 acgtcaccct gaccgccgag cagatccagg cctacggcgc ctccgacatc gccgaactgc
     4921 tgacctatct ggagcctctc acccgcagct cgcgtggccg cggcgacggc cagcccgtgc
     4981 tgctggtcaa tggccgccgc atctccggct tccaggaaat ccagggcatc ccgaccgagg
     5041 ccatcgaacg caccgacatc ctgcccgagg aagtcgcgct gcaatacggc tatcgcgccg
     5101 accagcgcgt cgtgaatttc gtgctcaagt cgaatttccg ctcgctcgtg ggccagctct
     5161 cgggccgcgc cccgacccag ggcggccgct ccatcggcga gatcgagggc aacgtcctgc
     5221 gcatcgccgg cgccacccgc tggtcggtgg acgcggaata cgaacgctcg accccgctgt
     5281 acgaatccga gcgcgacatc gagcgcgatc cgtccgccgg cgagttcgga gaccccgacc
     5341 tgcgcggcgc ctaccgcacc ctgctgtcag agagcgagc
//
LOCUS       DASVVN010000617         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_77729, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000617 DASVVN010000000
VERSION     DASVVN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77729"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VF686_10035"
     CDS             complement(<1..581)
                     /locus_tag="VF686_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8662193.1"
                     /translation="MAGFGSSFGRGGNAGGPRPARTPLQRLIYWAAVLAVWGLIFLVV
                     FFAVFARDLPDTSTLYNVDRQPSITYLDRNGALIATRGTQQAPPADLDALPDYVPAAF
                     IAIEDRRFYHHPGFDPVGMTRAMAANMRAGRVVQGGSTLTQQLAKNLFLSPDQNIKRK
                     VQELMLAVWLEMKFSKEEILALYLNRVYFGAGA"
     gene            complement(699..1652)
                     /locus_tag="VF686_10040"
     CDS             complement(699..1652)
                     /locus_tag="VF686_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8662194.1"
                     /translation="MKTMTAAAALAGALCLIAAPAMAQEHPQHQPAAPAQGHAHGHAH
                     ADFESGRFHVRVDGPEHPVGDVILIPGLSSSPEVWDGLTDQLKGRYRVHRIHIQGFAG
                     AAAEDNATGPVSAPVAEDIARYIAEKGLNKPAVIGHSMGGTIGMMLAARHPDSVGKLM
                     VVDMVPFMGAMFGAPGATAESVTPTADQIYAAMANGPEESYRQQAAASITGMINTESL
                     RAGPLEDAANSNRAVSASAFRELIVTDLRPELPRITAPTTVLYVRFNDPRMTNDMVDG
                     IYRASFSTLPSAQLKRIDDSAHFIMLDQPAVFAAEVNAFLE"
BASE COUNT          308 a          591 c          721 g          306 t
ORIGIN      
        1 taggcgccgg cgccgaaata gacgcggttc agatagaggg cgaggatctc ttccttggag
       61 aatttcatct ccagccacac ggccagcatc agctcctgga ccttgcgctt gatgttctgg
      121 tccggggaca ggaacaggtt cttggccagc tgctgggtca gggtggagcc gccctggacg
      181 acgcggccgg cgcgcatgtt ggcggccatg gcgcgggtca tgccgacggg gtcgaagccc
      241 gggtggtgat agaagcggcg gtcctcgatg gcgatgaagg cggccgggac gtagtccggc
      301 agggcgtcga ggtcggccgg cggggcctgt tgcgtgccgc gggtggcgat cagggcgccg
      361 ttgcggtcca gataggttat cgagggctgg cgatcgacat tgtacagcgt cgaggtgtcg
      421 ggcaggtcgc gcgcgaacac cgcgaagaag acgacaagga agataaggcc ccagacggcg
      481 aggacggcgg cccagtagat caggcgttgc agaggcgtgc gcgcgggccg gggaccgccg
      541 gcattgccgc cgcgaccgaa gcttgagccg aaacccgcca tgaaaacctc acctgaccct
      601 gaccggtcct agccgatcag ggcgcgaacg gaaacgcccc cgcttcagtc acaggactgt
      661 gaagagcgtt tccgccgaaa ccatgacgct tcaggcccct attctaaaaa cgcgttcacc
      721 tcggcggcga agacggcggg ctggtccagc atgatgaagt gagcgctgtc gtcgatccgc
      781 ttcagctgcg ccgagggcag ggtcgagaag gaggcgcggt agatgccgtc caccatgtca
      841 ttggtcatgc gcggatcgtt gaaccggacg tagagcacgg tggtcggggc ggtgatgcgc
      901 ggcagttccg gccgcaggtc ggtgacgatc agctcgcgga aggccgaggc cgagacggcg
      961 cggttgctgt tcgcggcatc ctcgagcggg ccggcgcgca gcgattccgt attgatcatg
     1021 cccgtgatgg aggcggcggc ctgctggcgg taggattcct cggggccgtt ggccatggcg
     1081 gcgtagatct gatccgccgt cggggtgacg ctttcggccg tggcgcccgg cgcgccgaac
     1141 atcgcgccca tgaagggcac catgtcgacg accatcagct tgccgacgct gtcgggatgg
     1201 cgggccgcca gcatcatgcc gatggtcccg cccatggaat ggccgatgac ggccggcttg
     1261 ttcaggccct tctcggcgat gtagcgggcg atgtcctcgg ccaccggggc ggaaaccgga
     1321 cccgtggcgt tgtcctcagc cgccgcgccg gcgaagccct gaatgtggat gcggtggacc
     1381 cggtaccggc ccttgagctg gtccgtcagg ccgtcccaga cctcgggcga ggagctgaga
     1441 ccggggatca ggatgacgtc gccgaccgga tgctcgggcc cgtcgacgcg gacgtggaag
     1501 cggccggatt cgaaatcggc gtgggcatgg ccatgcgcgt gaccttgggc gggggccgcc
     1561 ggctgatgct gcgggtgctc ctgcgccatc gcgggcgcgg cgatcaggca gagggccccg
     1621 gcgagggcgg cggcggcggt catggtcttc atggtcggat ccccggaatg agccggcgat
     1681 gcgcccgctt ctgtccacta gtcgccgcag agggcgcgaa cggatgcagc cggtcaagac
     1741 ttcgcgcacc ttgcccgcta aactggcgtc atgagctcag ccgacagcgc ccccgtttcc
     1801 gccaattcgc ccgaggccaa ggcgccgccg acggcgttcg gcaaggggtt cgtgctggac
     1861 gcctggtatt tcgtggccct ggcgcgggac gtggctccgg cctcgctgaa gcgccacgag
     1921 ctgctg
//
LOCUS       DASVVN010000618         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_101782,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000618 DASVVN010000000
VERSION     DASVVN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101782"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF686_10045"
     CDS             complement(<1..629)
                     /locus_tag="VF686_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX8662195.1"
                     /translation="MSWRRLTFRQWVGWIGLTIILLLATAVAVWRGDILKAGLDPQVP
                     FQTYTPPPAPDYGAPAAWALRETRTPGAGPAAIFFVHSTTYDGGRHWNGPVGDRAADA
                     YLSRVVLPNYAGPFARAGAISAPRYRQGSLYTRLTLREDAREARAFAYGDAAAAFDVW
                     IANHPTGPIVLAGVEQGGDIVERLVRERVATDPALRRRLVAAYLIDVVAA"
     gene            complement(626..1627)
                     /gene="rfaD"
                     /locus_tag="VF686_10050"
     CDS             complement(626..1627)
                     /gene="rfaD"
                     /locus_tag="VF686_10050"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX8662196.1"
                     /translation="MIRRMIVVTGGAGFIGSNIVARLCAEGRRDIVVCDRLETADLGK
                     WKNLAKHPIADFWEPEELFDQLERNAERIEAVVHMGAISSTTEQDADLILRTNFSLSR
                     DLWAWCALRDARLIYASSAATYGDGGLGFEDQDDLRSLSALRPLNAYGYSKYLFDQYA
                     ARQAQSRTAPTQWAGLKFFNVYGPNEYHKGGMKSVVAQIWPKVQADQPVTLFRSHNLN
                     YADGGQMRDFVLVDDVVDMIEWLLETPEVSGVFNAGSGQARSFLDLANATFAAAGKTP
                     RIEYVDTPESIRDKYQYFTEARMERVRAAGFNGQSTPLEEGVRRYVQDFLAKADPYR"
     gene            complement(1624..>2197)
                     /gene="rfaE2"
                     /locus_tag="VF686_10055"
     CDS             complement(1624..>2197)
                     /gene="rfaE2"
                     /locus_tag="VF686_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX8662197.1"
                     /translation="ILASGVVVGKSGTAVVTPAELIDAEMSQHAAGAHAKVMPLEELA
                     HVVEGWKRQRLKVGFTNGCFDILHRGHVAYLAQARGWCDRLVVALNTDASVRRLKGEG
                     RPINDLDSRAVVIGGLQSVDRVTAFDDPTPLALIERLRPDVLIKGADYTREAVVGGDL
                     VESWGGEVKLATFEDGYSTTKTVEKMKGGT"
BASE COUNT          355 a          810 c          679 g          353 t
ORIGIN      
        1 gccgccacta catcgatcag ataggccgcg accagccgcc ggcgcagcgc cgggtcggtc
       61 gccacccgtt cgcgcaccag ccgctcgacg atgtcgccgc cctgctccac gccggccaac
      121 acgatcggcc ccgtcggatg gttggcgatc cagacatcaa aggccgccgc cgcatcgcca
      181 taggcgaagg cccgcgcctc tcgcgcgtcc tcgcgcaggg tcagccgggt atagagactg
      241 ccctgccggt agcgtggggc gctgatcgcc cccgcccgcg cgaacgggcc ggcgtagttg
      301 ggcagcacca cccggctcag gtacgcgtca gcggcgcggt ccccgaccgg tccgttccag
      361 tgtcgcccgc cgtcataggt ggtcgaatgg acgaagaaga tcgccgcggg ccccgcgccc
      421 ggcgttcgcg tctcccgcag cgcccaggcc gccggcgcgc cgtagtcggg cgcgggcggc
      481 ggcgtatagg tctggaacgg cacctgcgga tccagcccgg ccttgaggat gtccccgcgc
      541 cacacggcca ccgcagtggc cagcaggagg atgatcgtca ggccgatcca gccgacccac
      601 tgccgaaagg tcagccggcg ccagctcatc gatacggatc cgccttggcc aggaagtcct
      661 gcacatagcg gcgcacgccc tcttccagcg gcgtcgactg accattgaag ccggcggcgc
      721 gcacccgctc catccgggcc tccgtgaaat actggtattt gtcgcggatg ctctcgggcg
      781 tatcgacgta ttcgatccgc ggggtcttgc ccgccgccgc gaaggtcgca ttggccagat
      841 cgaggaagga ccgcgcctgc ccggatccgg cgttgaacac ccccgacacc tcgggcgtct
      901 ccagcagcca ttcgatcatg tcgaccacat cgtcgacgag gacgaagtcg cgcatctggc
      961 cgccgtcggc gtagtttaga ttgtgcgagc ggaacagggt caccggctgg tcggcctgca
     1021 ccttcggcca gatctgggcc acgaccgact tcatcccgcc cttgtgatac tcgttcggcc
     1081 catagacgtt gaagaatttc aggcccgccc actgggtcgg cgccgttcgc gactgcgcct
     1141 gccgcgccgc atactgatcg aacagatatt tcgaatatcc ataagcattt agcggcctga
     1201 gcgcagacag ggagcgaaga tcgtcctgat cctcgaaccc cagaccgcca tcgccgtagg
     1261 tcgccgccga cgaggcgtag atcaaccgcg cgtcgcgcag ggcgcaccag gcccacaggt
     1321 cacgcgacag gctgaaattg gtgcgcagga tcaggtccgc atcctgctcg gtcgtcgagg
     1381 aaatggcgcc catatgcacc accgcctcga tccgctcagc attccgctcc agctggtcga
     1441 acagctcttc cggctcccag aagtcggcga tcggatgctt cgccagattc ttccacttgc
     1501 ccagatcggc tgtttccagc cggtcgcaga cgacgatgtc gcgccgtcct tcggcgcaca
     1561 ggcgcgcgac gatgttcgag ccgatgaagc cggccccgcc cgtcaccacg atcatgcgcc
     1621 ggatcatgtt ccgcccttca tcttctcgac cgtcttcgtc gtcgaatagc cgtcctcgaa
     1681 cgtggccagc ttcacctcgc cgccccagct ctcgaccaga tcgccgccca ccacggcttc
     1741 ccgcgtatag tccgcgccct tgatcagcac gtccggccgc agccgctcga tcagggccag
     1801 cggcgtggga tcgtcgaagg cggtgacccg gtccacggac tgaagcccgc cgatgaccac
     1861 ggcccggctg tccagatcgt tgatcggccg cccctcgccc ttcaggcggc gcaccgaggc
     1921 gtcggtgttc agcgccacca ccagccggtc gcaccagccg cgcgcctggg ccagataggc
     1981 cacgtggccg cggtgcagga tgtcgaaaca gccgttggtg aagccgacct tcagcctctg
     2041 cctcttccag ccctcgacga cgtgcgccag ctcttccagc ggcatgacct tggcgtgggc
     2101 tccggccgca tgctggctca tctccgcgtc gatcagttcg gcgggcgtga ccacggcggt
     2161 cccggacttt ccgaccacca cgcccgaggc caggatg
//
LOCUS       DASVVN010000619         5487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_155885,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000619 DASVVN010000000
VERSION     DASVVN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155885"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..805
                     /locus_tag="VF686_10060"
     CDS             182..805
                     /locus_tag="VF686_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8662198.1"
                     /translation="MSAPATHEPSLHDPKREIILRHARRAFTTEGYAGARIEPIARDA
                     GVSTATIYALFDGKEELFAAVIEDAAIEFTRLMGDVRTTSGPARERLMCFATTYAAFM
                     ADPFIRAVFRLVMAERRRFQEVALKFFDRGKADFGKVLIDALNEMVGTGELRVERPSW
                     AAGQLMGMIEHPVFFMPLVTGDEVMPVRAVNQIASDAVETFLARYGV"
     gene            complement(810..1484)
                     /gene="pdxH"
                     /locus_tag="VF686_10065"
     CDS             complement(810..1484)
                     /gene="pdxH"
                     /locus_tag="VF686_10065"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX8662199.1"
                     /translation="MTAPVIPSSPTREEYARDYAAALAANGDETIFERAEPISLFIDW
                     LNDAKAAEPNDANAMALSTVDGDGAPDSRMVLLKDVDARGFTFYSNAGSAKGDQLAVR
                     PRAALLFHWKSLRRQVRVRGRVEPVTADEADVYFASRARESRIGAWASDQSRPLESRD
                     ALEVAVARETARFEGGDVPRPEHWTGWRVVPDQVEFWRDRPFRLHDRLRFDRDGADWT
                     RTRLQP"
     gene            1571..2296
                     /locus_tag="VF686_10070"
     CDS             1571..2296
                     /locus_tag="VF686_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX8662200.1"
                     /translation="MRARSLPEVVSTPDAYALLGLTGPAESHVLTAAFRKAVKAARPD
                     APGGSEARFRKVIAAWRLIQQETAPLALAPPVRSAPALPVVGLSPETALNGGMVEVRL
                     GRRTLRVRVPAGLRSGEHLRLKAAGDAGADLYLPVLVRGDGRLSAVGDDLYMTFPVPP
                     RLLEDGGRLEIETHAGPRSAWLVAGCQPLRLRLRGLGLPARGARPQGHLFVTLEPSED
                     APSAAEDLLVRFTRVWTPDRLAA"
     gene            2309..3394
                     /gene="aroC"
                     /locus_tag="VF686_10075"
     CDS             2309..3394
                     /gene="aroC"
                     /locus_tag="VF686_10075"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX8662201.1"
                     /translation="MSHNTFGHLFRVTTWGESHGPAIGCVVDGCPPLIPLTEGDLQPW
                     LDRRKPGGSRFVTQRQESDTARILSGVFDDGNGPVTTGAPIAIQIDNEDARSKDYGEI
                     ARAFRPGHADYTYQAKYGVRDHRGGGRSSARETAVRVAAGAVARRILGDGVRVRAGVI
                     QLGPHRIAPEAVDFDAVYDNALFAASAAVVPAWEDYLDGVRKAGSSIGAVVALEVTGV
                     PVGWGAPVYGKLDAELAAAMMSINAVKGVEIGAGFGSAELSGEENADQMRIGDDGRAV
                     FLSNHAGGVLGGISTGQPVTARIAFKPTSSILTPRQTITRDGEETDLRTKGRHDPCVA
                     LRGVPVVEAMAACVLADAMLRHRAQVG"
     gene            3473..4168
                     /locus_tag="VF686_10080"
     CDS             3473..4168
                     /locus_tag="VF686_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8662202.1"
                     /translation="MIDVRPFSSLGGANHGWLNAKHHFSFANYYDPSRMSWGRLRVWN
                     DDEIAAQSGFPPHPHSDMEIITYVRTGAITHEDSIGNRGRTGAGDVQVMSAGTGVRHS
                     EFNLENETSTLFQIWIEADKRGATPSWGAKVFPKHDRTGKFSVLASGDPTDDALTINA
                     DARVLGATLNAGESLTYSLGEGRRAYLVPAVGEVEVNGVPLNARDGAAIKDEAEITIT
                     AKADAELVMVDSL"
     gene            complement(4165..4788)
                     /locus_tag="VF686_10085"
     CDS             complement(4165..4788)
                     /locus_tag="VF686_10085"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8662203.1"
                     /translation="MTTADPHHLRRVQLIRKTALLFSLVVVIGLAALSQSLGGETVLH
                     EGLEAVGLAMIAICVVGRAWCSLYIGGRKKAEIVDQGPYSVSRNPLYVFSFIGAFGMG
                     AQTGSVVMAMLFLLISIGVFYLTVRQEEGWLSATFGAAYRAYCARTPRFWPDFSKWRD
                     AETLEVRPVFFLTTLRDGLGFFLAVPLFEALEHAQTVGWVRVLFSLP"
     gene            complement(4882..>5487)
                     /locus_tag="VF686_10090"
     CDS             complement(4882..>5487)
                     /locus_tag="VF686_10090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX8662204.1"
                     /translation="WTEFGGEILTIEAIKMPGRGRMTVTGNLKDVMKESISAAASYVR
                     ARSLSIGVKPPAFEKTDIHVHVPDGATPKDGPSAGVAMTVAMVSVLTGIPIRKDIAMT
                     GEITLRGRVTAIGGLKEKLLAALRSGIKTVLIPEENEKDLADVPDNVKSALEIVPIKT
                     ADEALRWALTGPLTPVEWDEASEPLPAAQPIQDPGTVPATH"
BASE COUNT          885 a         1932 c         1838 g          832 t
ORIGIN      
        1 gacgccaccg ccgccgtgcg gctggaagat ccgcaggccg tggtccggct ggtcgcaccc
       61 gtcctggccc atccgctgct cgtggagcta taccactcat gattgcatag aaacagttgt
      121 tttttcttgc ccctcgtcag atgtggggtc actttgagga ttcagcgagc agcgaattcc
      181 aatgtcggcc cccgccacgc acgagccttc tctccacgac cccaagcgcg agatcatcct
      241 gagacacgcg cggcgcgcgt tcaccacgga aggttacgcc ggcgcgagga tcgagcccat
      301 cgcgcgcgac gccggagttt ccaccgcaac catctacgcc ctgttcgacg gcaaggagga
      361 actattcgcg gcggtgatcg aggacgccgc catcgaattc acccgcctga tgggcgacgt
      421 tcgcacgacc agcggccccg cacgggaacg gctgatgtgt ttcgccacca cctacgccgc
      481 cttcatggcc gatcctttca tccgggcggt cttccggctc gtcatggccg agcggcggcg
      541 gttccaggaa gtggccctga aattcttcga ccggggcaag gccgatttcg gcaaggtgct
      601 gatcgacgcc ctgaacgaaa tggtcggaac gggcgagctc agggttgaac ggccctcctg
      661 ggcggccgga cagctgatgg ggatgatcga gcatccggtc ttcttcatgc ccctggtgac
      721 gggggacgag gtcatgcccg tacgcgccgt gaaccagatc gcgagcgacg cggtggagac
      781 cttcctggct cgctacggcg tctgaaggat cacggctgaa gccgggtccg cgtccagtcc
      841 gcgccgtccc ggtcgaatcg cagccggtcg tgcaagcgga acggccggtc gcgccagaac
      901 tcgacctgat ccgggaccac gcgccatccg gtccagtgct cgggccgcgg cacgtcaccg
      961 ccctcgaacc gcgccgtttc gcgcgcgaca gccacctcaa gcgcatcccg gctctccagc
     1021 ggccgcgact ggtccgaggc ccaggcgccg atccggctct cgcgcgcccg gctggcgaaa
     1081 tagacgtcgg cctcgtcggc ggtcaccggc tcgacccgcc cccgcacccg cacctgccgg
     1141 cgcagggact tccagtggaa cagcagggcc gcccgcggcc gtacggccaa ctggtcgccc
     1201 ttggcgctcc cggcattgga atagaaggtg aagccgcgcg catcgacgtc cttcagcagc
     1261 accatccggc tgtccggcgc cccgtccccg tcgacggtcg acagggccat ggcgttggcg
     1321 tcgttgggct cggccgcctt cgcatcgttc agccaatcga taaacaggga aatcggctcg
     1381 gcacgctcaa agatcgtctc gtcgccgttc gcggccaggg cggcggcgta gtcgcgcgca
     1441 tattcctcgc gagtcgggct ggacgggatc acgggcgcgg tcatgggacc gagatagtgc
     1501 atcgcgggcg atcgggcgag agcgtcacgg ttaaccgggc attgaccctg ttgggggcct
     1561 tagtggcgac atgcgcgccc gatcgctccc tgaggtcgtc tcgacccctg acgcctacgc
     1621 cctgctcggc ctcaccggcc cggcggagag ccatgtcctc accgccgcct tccgcaaggc
     1681 cgtcaaggcg gcgcggcccg acgcgccggg cggaagcgag gcccggttcc gcaaggtcat
     1741 cgccgcctgg cgtctgatcc agcaggagac ggcgccgctc gccctcgccc cgccggtgcg
     1801 ctccgccccc gccctgcccg ttgtcggcct cagcccggag accgcactca acgggggcat
     1861 ggtcgaagtc aggctgggcc gccggacgct gcgggttcgg gtccccgcgg gcctgcgcag
     1921 cggcgagcat cttcggctga aggcggcggg agacgccggc gccgacctgt acctgcccgt
     1981 ccttgtgcgc ggcgacggcc ggctcagcgc ggtcggcgac gacctctaca tgaccttccc
     2041 ggtgcctccg cgtctgctgg aggatggcgg gcggctggag atcgagaccc atgcgggacc
     2101 gcgctccgcc tggctggtcg cgggctgtca gccgctgcgg ctgcgcctca ggggactggg
     2161 cctgcctgcg cgaggcgccc ggccccaggg ccacctgttc gtgacgctcg aaccgtccga
     2221 ggatgcgccg tcggcggccg aggatctgct ggtccggttc acgcgcgtct ggacgccgga
     2281 ccggctggcg gcctaagccg tacaggtcat gtcgcacaac accttcggcc atctgtttcg
     2341 cgtgacgacc tggggcgaga gccacgggcc ggcgatcggc tgcgtcgtcg acggctgccc
     2401 gcctctgatc ccgctcactg agggcgattt gcagccctgg ctggaccggc gcaagcccgg
     2461 cggcagccgc ttcgtcaccc agcggcaaga gagcgacacc gcccgcatcc tgtcgggcgt
     2521 gttcgacgat ggaaacggcc cggtgaccac cggcgccccc atcgccatcc agatcgacaa
     2581 cgaggacgcc cggtcgaagg actatggcga gatcgcccgc gccttccggc ccggccacgc
     2641 cgactacacc tatcaggcca aatacggcgt ccgggaccat cgcggcggcg ggcgctcgtc
     2701 ggcgcgcgag accgccgtgc gggtcgcggc cggagccgtg gcgcgcagaa tcctcggcga
     2761 cggcgtgcgc gtccgggccg gcgtgatcca gttgggcccg caccggatcg ccccggaggc
     2821 ggtcgatttc gacgccgtct acgacaacgc cctgttcgcg gcttccgcgg cggtggttcc
     2881 ggcgtgggaa gactacctcg acggcgtccg taaagctggc tcatccatcg gcgcggtggt
     2941 ggcgctggaa gtcaccggcg tcccggtcgg ctggggcgcg ccggtctacg gcaagctcga
     3001 cgccgaactg gccgccgcca tgatgtcgat caatgcggtc aagggcgtcg agatcggcgc
     3061 cggcttcggt tcggccgaac tgtcgggcga ggagaacgcc gaccagatgc gcatcgggga
     3121 cgacgggcgg gccgtcttcc tgtccaacca tgcgggcgga gtcctgggcg gcatctcgac
     3181 cggccagccg gtcaccgccc gcatcgcctt caagcccacc tcctccatcc tgacgccgcg
     3241 ccagaccatc acccgcgacg gcgaggagac ggacctgcgc accaaaggcc gccacgaccc
     3301 ctgcgtcgcc ctgcgcggcg tgccggtggt cgaggccatg gcggcgtgtg tgctggcgga
     3361 cgcgatgctg agacaccggg cgcaggtcgg ctgacccctt ttcagccacc aacactgtta
     3421 ctatatacgc gccagcgagg ttcataaccc tcggattccc gggagcatcc ccatgatcga
     3481 cgtcagaccc ttctccagcc tcggcggcgc gaaccacggc tggctgaacg ccaaacacca
     3541 tttctccttc gccaactact atgacccgag ccgcatgagc tggggccggc tgcgtgtctg
     3601 gaacgacgac gagatcgccg cccagtcggg cttcccaccc cacccgcact cggacatgga
     3661 gatcatcacc tacgtccgta cgggcgccat cactcacgag gattccatag gcaaccgggg
     3721 ccgtacgggc gccggcgacg tccaggtgat gagcgccggc accggggtgc gtcactccga
     3781 gttcaatctc gagaacgaga cctccaccct gttccagatc tggatcgagg ccgacaagcg
     3841 cggcgcgacg cccagctggg gcgccaaggt gttccccaag cacgaccgca cggggaagtt
     3901 ttccgtgctg gcgtcgggtg acccgaccga tgacgccctg accatcaacg ccgacgccag
     3961 ggtgctgggc gcgacgctga acgcgggcga gagcctgacc tacagcctcg gcgaaggccg
     4021 ccgcgcctat ctggtccccg ccgtcggcga ggtcgaggtc aacggcgtgc ccctgaacgc
     4081 ccgcgacggc gccgccatca aggacgaggc cgagatcacc atcacggcga aggccgatgc
     4141 ggaactggtg atggtcgaca gcctctaggg caggctgaac agcacgcgca cccagcccac
     4201 ggtctgggcg tgctcaagcg cttcgaacaa cgggacggcc aggaagaatc cgaggccgtc
     4261 ccgcaaggtg gtcaggaaga agacgggccg gacctccagc gtttcggcgt cgcgccattt
     4321 cgaaaaatcg ggccagaacc gcggcgtacg ggcgcaatag gcacggtagg cggcgccgaa
     4381 ggtcgccgac agccatccct cctcctgccg caccgtcaga tagaagaccc cgatcgagat
     4441 cagcaggaac agcatggcca tgaccacgct gcctgtctgc gcgcccatgc cgaaggcgcc
     4501 gatgaagctg aagacgtaga gcgggttgcg gctgaccgaa taaggaccct ggtcgacgat
     4561 ctcggccttc tttcgcccgc cgatatagag cgagcaccag gcgcggccga ccacgcagat
     4621 ggcgatcata gccaggccga cggcctcaag accctcatgc aacaccgtct cgccgccgag
     4681 actctggctc agggctgcca ggccgatcac cacgaccagc gagaacaaca acgctgtctt
     4741 gcggatcagc tggacgcggc ggaggtggtg ggggtcggcg gtggtcattg gcaaggctag
     4801 agcccgctcc acgtcaacat caaggcaaag cttcgccgcc aaagaaaaac gccgccccgg
     4861 tttcccgagg cggcgctggt tctagtgagt agcaggcacc gtgcccgggt cctggatcgg
     4921 ctgggcggcc gggaggggct cggaggcctc gtcccattcg accggcgtca gcgggccagt
     4981 cagagcccag cgcagggcct cgtcggccgt cttgatcggg acgatctcca gcgccgactt
     5041 cacattgtcg ggcacgtcgg cgaggtcctt ctcgttctct tcggggatca gcaccgtctt
     5101 gatgcccgag cggagggccg caagcagctt ctccttcagg ccgccgatgg cggtgacccg
     5161 gccgcgcagg gtgatctcgc cggtcatggc gatgtccttg cggatcggga ttcccgtcag
     5221 gaccgaaacc atggcgaccg tcatggccac gcccgccgag ggaccgtcct tgggcgtcgc
     5281 gccgtccggc acgtgcacgt ggatgtcggt cttctcgaac gccggcggct tgacgccgat
     5341 gctgagcgaa cgcgcccgga cgtaggatgc ggcggccgag atggactcct tcatcacgtc
     5401 cttcaggttg ccggtcacgg tcatgcggcc gcggcccggc atcttgatcg cctcgatggt
     5461 caggatctcg ccgccgaact cggtcca
//
LOCUS       DASVVN010000620         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_155886,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000620 DASVVN010000000
VERSION     DASVVN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155886"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VF686_10095"
     CDS             complement(<1..759)
                     /locus_tag="VF686_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX8662205.1"
                     /translation="MARAKIALIGAGMIGGTLAHVAAREALGDVILFDIAEGTPQGKA
                     LDIHQGSAVFGQDVSLKGANDYADIAGADVCIVTAGVPRKPGMSRDDLIGINLKVMKA
                     VGEGIKQHAPNAFVICITNPLDAMVWALQKFSGLPKEKVVGMAGVLDSARFAYFLAEK
                     TGVSVQDIHAWTLGGHGDDMVPMVRHSTIGGLPLPDAVKAGFMTQEELDAIVDRTRKG
                     GGEIVGLMGTSAYYAPAASAIAMAEAYLLDQKRLV"
     gene            927..1718
                     /locus_tag="VF686_10100"
     CDS             927..1718
                     /locus_tag="VF686_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX8662206.1"
                     /translation="MASLPEIVAAEDTPAGLITVVDEATVRAEVEAWRAKVLKRPGLW
                     DRATRGTQERINRIIPERVHAVITAGVKAMTKGILFGSDLVTPRPMADGSLAARELRA
                     RAMIRTYRTTAGVEGGVAGAGGFVLAAADVPALMAIKVRMLGDVVGAYGWGGDTNGDS
                     GRERLFVLHIFHLAFASAKRRPEALAELERWIAGADQPQAITDYDWRRFQIEYRDYID
                     LAKMAQLIPVVGAPIGAVVNWRLVDRLGETAMMACRMRWMAEAAG"
     gene            complement(1715..2929)
                     /locus_tag="VF686_10105"
     CDS             complement(1715..2929)
                     /locus_tag="VF686_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8662207.1"
                     /translation="MRILLSTYGSRGDVEPLVGLAVALQSLGAEAVVSAPPDQEFVDL
                     LARAGVPLAPAFMSAREWIDGAKARPADGIPQHAARMIGAQYEAIDAAAADCDAIVAT
                     GLMPSAAAAQCVAEKRGLPYRHVTFCPLFLPSDHHRPFPYPGRPHPPEVTDPRALWDL
                     NIADMNALFGEAVNGQRAAIGLLPVDNVRDHVFTDRPLLASDPVLWPWEPTGLTDAVQ
                     TGAWILPDARALPADLEAFIAAGAPPVYVGFGSIALPSTREAARVAIEAARAHGRRVV
                     LARGWVDAAPIDEGDDVFLTGDINQQSLFPRMAAIVHHGGAGTTTAATRAGAPQVIVP
                     QIVDQPYWAARVAGLGVGVAHDGATPTLESLSAALATALAKEMSARAAAVSPTIRADG
                     ALTAAKRLLDEL"
     gene            complement(3029..>3587)
                     /locus_tag="VF686_10110"
     CDS             complement(3029..>3587)
                     /locus_tag="VF686_10110"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX8662208.1"
                     /translation="SWAAMTLAALVFGLYASPWWGPLTAPLASVPAAVLLFGLYGLGA
                     AAMASLQPAQGAMGKAATAGTVGILFVLMTLLIRWVFHGAAMSVPAPGGGLETWTFSA
                     LWAVFGLAVLSLGAARHDVALRWAGLAVLLFTAAKVMLFDLARLEGVIRAASFLAVGA
                     LFLAGAIAARRLNNRGSTREDADTP"
BASE COUNT          539 a         1174 c         1364 g          510 t
ORIGIN      
        1 caccagccgc ttctgatcga gcaagtaagc ttcggccatc gcgatcgcgc tggcggcggg
       61 cgcgtagtag gcgctcgtcc ccatcagacc gacgatctcg ccgccgccct tgcgcgtgcg
      121 atcgacgatg gcgtccagct cttcctgggt catgaagccg gccttgaccg cgtcgggcag
      181 gggcaggccg ccaatggtcg agtggcgcac catcggcacc atgtcgtcgc cgtgaccgcc
      241 cagggtccag gcgtggatat cctgcaccga gacgccggtc ttctcggcga ggaaatacgc
      301 gaagcgggcc gagtcgagca cgccggccat gccgacgacc ttctccttgg gcaggcccga
      361 gaatttctgc agggcccaga ccatggcgtc gagcgggttg gtgatgcaga tgacgaaggc
      421 gttcggggcg tgctgtttga tgccctcgcc gacggccttc atgactttca gattgatgcc
      481 gatcaggtcg tcgcggctca tgcccggctt gcgcggcacg ccggcggtga cgatgcagac
      541 gtcggcgccc gcaatgtcgg cgtagtcgtt ggcgcccttc aggctgacgt cctggccgaa
      601 gacggccgag ccctggtgga tgtccagcgc cttgccctgg ggggtgccct cggcgatgtc
      661 gaacaggatg acgtcgccca gcgcctcacg cgccgccaca tgggccaggg tgccgccgat
      721 catgccggcg ccgataaggg cgatcttcgc gcgagccata gagttctctc ttttgtttgg
      781 cctaccgcgc ttcgcgctac ttgaggccga tcagggccag cggcgtttcg cgtcggtccg
      841 tgtgggtggg gagggaaagc gttggcggcg gtctagaccc gctcgcattc gcctgcaacc
      901 gtcacgccgc gtcacccgga gccgtcatgg ccagcctgcc cgaaatcgtc gccgccgaag
      961 acacgcccgc cggactgatc acggtcgtgg acgaagcgac ggtgcgcgcc gaggtcgagg
     1021 cgtggcgggc gaaggtgctg aagcggccgg gcctgtggga ccgggccacg cgcgggacgc
     1081 aggagcggat caaccggatc atccccgagc gggtgcatgc cgtcatcacc gccggcgtca
     1141 aggcgatgac gaaggggata ttgttcggct cggatctggt cacgccgcgt ccgatggcgg
     1201 acggatcgct ggcggcgcgc gagctgaggg cgcgggcgat gatccggacg tatcgcacca
     1261 cggcgggcgt cgagggcggg gtcgcggggg cgggcggttt cgtgctggcg gcggcggacg
     1321 ttccggccct gatggcgatc aaggtgcgga tgctgggcga cgtggtcggc gcctatggct
     1381 ggggcggcga cacaaatggc gactccgggc gtgagcggct gttcgtgctg cacatctttc
     1441 atctggcctt cgccagcgcc aaacggcggc ccgaagcgtt ggctgagctg gagcggtgga
     1501 tcgccggcgc cgaccagccg caggcgatca ccgactacga ctggcggcgg ttccagatcg
     1561 agtatcggga ctacatcgat ctggccaaga tggcccagct gatccccgtc gtcggcgcgc
     1621 ccatcggggc ggtggtaaac tggcggctgg tcgaccgtct gggcgagacg gccatgatgg
     1681 cgtgccggat gcgctggatg gcggaggccg cgggctagag ttcgtccagc agccgtttcg
     1741 ccgccgtgag cgcgccgtcg gcgcggatcg tcggcgagac ggcggccgcg cgggcgctca
     1801 tttccttggc cagggcggtg gcgagagcgg cggagaggga ctccagggtg ggcgtggctc
     1861 cgtcgtgcgc gacgccgacg cccagtccgg cgacgcgggc ggcccagtac ggctggtcga
     1921 cgatctgggg gacgatgacc tgcggcgctc ccgcgcgggt ggcggcggtg gtcgtgcccg
     1981 cgccgccgtg atgcacgatg gcggccatgc gggggaacag cgactgctgg ttgatgtcgc
     2041 cggtcaggaa gacgtcgtcg ccctcgtcga tgggcgcggc gtcgacccag ccgcgcgcca
     2101 gaaccacgcg gcgaccgtgc gcccgggccg cctcgatcgc gacccgggcc gcctctcgcg
     2161 tggacggcag ggcgatgctg ccgaagccga cgtagacggg cggggcgccg gccgcgatga
     2221 aggcctcgag atccgcaggc agcgcgcggg cgtcgggcag gatccacgcc ccggtctgga
     2281 cggcgtcggt gaggccggtc ggctcccatg gccacaggac agggtcggag gcgagcagcg
     2341 ggcggtcggt gaagacgtgg tcgcggacat tgtccaccgg cagcaggccg atggcggcgc
     2401 gctggccgtt caccgcctcg ccgaacaggg cgttcatgtc cgcgatattg agatcccaca
     2461 gcgcgcgggg gtccgtcacc tccggcgggt gaggacggcc cggataggga aagggccggt
     2521 ggtggtccga gggcaggaac agcgggcaga aggtgacgtg ccggtagggc aggccccgct
     2581 tttcggccac gcattgcgcg gcggcggcag agggcatcag gccggtggcc acgatggcgt
     2641 cgcagtccgc ggcggccgcg tcgatcgcct cgtactgggc cccgatcatc cgcgcggcat
     2701 gctgggggat gccgtccgcc ggccgcgcct tcgcgccgtc gatccattcg cgcgccgaca
     2761 tgaaggccgg agccagcgga acgccggcgc gggccagcag gtcgacgaac tcctggtccg
     2821 gcggtgctga cacgaccgct tcggcgccga gtgattgcag ggccacggcc agtccgacca
     2881 gcggctcgac atcgccgcgg gacccgtagg tcgacaacag gatacgcatg gggcggccct
     2941 ccgggttcaa gggctccgca gtgtgcgcat aaaaacgcgt tcgaccattc ttgaacgaaa
     3001 ctgttttgac cccaactcca gtctgacctt acggcgtatc ggcgtcctcg cgtgtcgagc
     3061 cccggttgtt caggcggcgt gcggcgatcg cccccgccag gaacagggcg cccacggcga
     3121 ggaaggaggc ggcgcgaatg acgccctcca gccgcgcgag gtcgaacagc atgaccttgg
     3181 ccgccgtgaa cagcagcacc gccagaccgg cccagcgcaa tgcgacgtca tggcgggccg
     3241 cgcccagact gaggacggcg aggccgaaca ccgcccacag ggccgagaag gtccaggtct
     3301 ccagcccgcc gccgggcgcc gggacgctca tggcggcgcc atggaagacc cagcggatca
     3361 gcagcgtcat caggacgaac aggatgccga cggttccggc cgtcgcggcc ttgcccatgg
     3421 cgccttgcgc aggctgcagg ctggccatgg ccgcggcgcc caggccgtag aggccgaaca
     3481 gcaggacggc ggccggcacg gaggcgaggg gcgcggtcag cgggccccac cacgggctgg
     3541 cgtagaggcc gaagacgagg gcggccagag tcatggcggc ccagctc
//
LOCUS       DASVVN010000621         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_470921,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000621 DASVVN010000000
VERSION     DASVVN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470921"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VF686_10115"
     CDS             <1..145
                     /locus_tag="VF686_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662209.1"
                     /translation="DRNDVEWMKHTLTWKTPGDQTKIDYRPVHNYTMSNDIGYIEPKA
                     RVY"
     gene            145..945
                     /locus_tag="VF686_10120"
     CDS             145..945
                     /locus_tag="VF686_10120"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX8662210.1"
                     /translation="MVQLTLPKGSKPTRGKVHKAPAGAKNVKTYKVYRYDPEVDADPR
                     WDTFEISADDHGPMLLDALIHIKNEIDPTLTFRRSCREGICGSCSMNIDGRNTLACTK
                     GWEECSSSTISIAPLPHQPVVKDLVTDLTLFYAQYDSIQPYLQSDEPDPEKERIQLPE
                     DRARLDGLYECILCACCSTSCPSYWWNQTEYLGPAALLQSYRWIADSRDDATDKRLDD
                     LEDPFKLYRCHTIMNCAQVCPKGLNPAKAIAETKKLMVSPSRKKTEAA"
     gene            1030..1590
                     /locus_tag="VF686_10125"
     CDS             1030..1590
                     /locus_tag="VF686_10125"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8662211.1"
                     /translation="MKRMMICAAAAALLSACAGMPTGEAAGVAQAAMERPAPAAPEFL
                     RMAGASDLYEIQSSQLVMQSTNEDVSGFARMMVDHHTMTTRTLTRAAQQAGMTPPAPA
                     LDARKAEMIRQLQAAQGEARDRLYVEQQVMAHQEALTLHTSYSRNGDTPELKTAAGAA
                     VPIVAQHYSRITAMQRDMAGSHADHS"
     gene            1704..>1868
                     /locus_tag="VF686_10130"
     CDS             1704..>1868
                     /locus_tag="VF686_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8662212.1"
                     /translation="MAKITYIEHDGREHVVEVKNGLSVMEGAIRNNVPGIDADCGGAC
                     ACATCHVYVDE"
BASE COUNT          366 a          653 c          583 g          266 t
ORIGIN      
        1 cgaccgcaac gacgtcgagt ggatgaaaca caccctgacc tggaagacgc cgggcgatca
       61 gaccaagatc gactaccgtc ccgtccataa ttacacgatg tccaacgaca tcggttacat
      121 cgaacccaag gcgcgggtgt actgatggtc cagctgacac tccccaaggg ctccaagccg
      181 acgcgcggca aggtccacaa ggctccggcg ggcgcaaaga acgtcaagac ctacaaggtc
      241 tatcgctacg accccgaggt cgacgccgac ccgcgctggg acacgttcga gatctcggcc
      301 gacgaccacg gcccgatgct gctggacgcc ctgatccaca tcaagaacga gatcgacccc
      361 accctgacct tccgccgctc gtgccgcgag ggcatctgcg gttcgtgctc gatgaacata
      421 gacgggcgca acaccctggc ctgcaccaag ggctgggagg agtgttcgtc ctcgaccatc
      481 tcgatcgcgc ccctgccgca ccagccggtg gtcaaggatc tggtcacgga cctgaccctg
      541 ttctacgccc agtacgactc gatccagccc tacctccagt ccgacgagcc ggatccggag
      601 aaggagcgca tccagctgcc cgaggaccgc gccaggctgg acggcctgta cgaatgtatc
      661 ctgtgcgcct gctgctcgac gtcctgcccc agctattggt ggaaccagac ggaatacctc
      721 ggcccggccg ccctgctgca gtcctaccgc tggatcgccg acagccgcga cgacgccacg
      781 gacaagcggc tcgatgacct cgaggacccg ttcaagctct atcgctgcca cacgatcatg
      841 aactgcgccc aggtctgccc caagggtctg aacccggcca aggccatcgc cgagaccaag
      901 aaattgatgg tttcgccctc gcggaaaaag accgaagcgg cctgatctgt ctggcctcgg
      961 acttaggaac ggggcctgtt gaacgtcgtt ggctcagcgg acgcgcgagg cgtctgaagg
     1021 agccgatcga tgaaacgaat gatgatctgc gcggccgccg ccgcgctgct ttccgcatgc
     1081 gcgggcatgc cgacaggcga ggcggcgggc gtcgcccagg ccgccatgga gcggcccgca
     1141 cccgccgccc cggaattcct ccgcatggcc ggggccagcg acctttacga gatccagtcc
     1201 agccagctgg tcatgcagtc gacgaacgaa gacgtcagcg gcttcgcccg catgatggtc
     1261 gatcaccaca ccatgaccac ccggaccctg acgcgggcgg cgcaacaggc gggcatgacg
     1321 ccgccggccc cggccctgga tgcgcgcaag gccgagatga tccgtcagct tcaggccgcc
     1381 cagggcgagg cccgcgaccg gctgtatgtc gagcagcagg tcatggcgca tcaggaggcg
     1441 ctgacccttc acaccagcta ttcccgcaat ggcgacacgc cggaactcaa gaccgcggcc
     1501 ggcgccgccg tgccgatcgt cgcgcagcac tatagccgga tcacggccat gcagcgggac
     1561 atggccggct cgcacgccga ccacagctga tccgcgtcgc ggcctctggc cccgcgccgg
     1621 gaggtcgcgc cgccgccggc gggcgggcag gaaatacgtt gctccgcatt gccacgcggg
     1681 aaatgcgcgc taagagcctc tcaatggcga agattaccta catcgagcac gatggccgcg
     1741 agcatgtggt cgaggtgaag aacggcctgt cggtcatgga aggggcgatc cgcaacaacg
     1801 tgccgggcat cgacgccgac tgcggcggcg cgtgcgcctg cgccacctgc cacgtctacg
     1861 tggacgag
//
LOCUS       DASVVN010000622         7073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_129903,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000622 DASVVN010000000
VERSION     DASVVN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129903"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1097)
                     /locus_tag="VF686_10135"
     CDS             complement(129..1097)
                     /locus_tag="VF686_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8662213.1"
                     /translation="MQSRHIQTDGLNQHILEAGTGPLVLLIHGFPELGISWRAQVEAL
                     AEAGFHAVAPDMRGYGSTEKPNDVPDYSILHLVGDMVDLVRALGETSCVVVGHDWGAP
                     VAWHCALTRPDVFSAVFGLSVPFQPRRPKGPPTAAMAAISKRLGLGDLYISRFQAADA
                     HAVFDAHPATALRKMFYAYDGATSDGRQSTGFIPEGQSFISTVPDDAPLPPWMSPEHL
                     AEYVEAFTAGGFKGPLDWYRNLDRNWSLTAHLQDARIRVPAAFVVGERDPVRHYAGQH
                     EAGLKDHAPDLRMQVVVPGAGHWIQQERPDEVNRLLLEFLREPGVG"
     gene            complement(1138..1779)
                     /gene="yihA"
                     /locus_tag="VF686_10140"
     CDS             complement(1138..1779)
                     /gene="yihA"
                     /locus_tag="VF686_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX8662214.1"
                     /translation="MDEFTPEELEQGRILFARPATFIMGCAKIEQLPDPDLPEVAFAG
                     RSNVGKSSLINGLVGMHKLARASNEPGRTREVNFFDLDGKMRLVDLPGYGWAKASKST
                     VKKFQDLGRDYLRGRVTLKRVYLLIDSRHGLKKVDDEALDALDLAAVSYQIILTKADK
                     LKKGEAEKVQAATLKAISKRPAAYPAVIVTSAEKGDGMPELRAEIMRTTGAEL"
     gene            complement(1783..3576)
                     /gene="yidC"
                     /locus_tag="VF686_10145"
     CDS             complement(1783..3576)
                     /gene="yidC"
                     /locus_tag="VF686_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX8662215.1"
                     /translation="MPPQDNSRNTILFVVIAAIMLMAYSYFVMQPQAERRKAAQQAAV
                     AERTAGAPAIATTPGAVTFTTDRTVALARGGARVPIKTGTVTGSLSLRGARIDDLFLT
                     NYQETMDEGSPAVELFRPEGMRHAYQAQFGWNGPNVPGGVPGPQTPWRLTEGATLTPT
                     TPVTLTWDNGAGLRFTRRIAIDAQYLFTVTDTVANFSAQPIAIAPWGRVERQGVPDGL
                     GKTQILHEGGIGTFGSDGNYTTEQLKYQGWAKKPVQEHTSTGGWMGITDKYWLAALIP
                     QQDERIQAAFRVTDAAHADIHSATMLGETHTIAPGRQITETQRLFAGAKRNEILSAYE
                     KDLGLPRFIYAIDWGFLFFLTRPIFLLIEFFYGIIGNFGLAILCLTLTVRLVMFPLAN
                     KSYESMSRMRTLQPKMEELKKKHPDDPAAQQKELMALYQKEKINPLAGCLPLLLQIPV
                     FYAVYKMLFVTIEMRHAPFFGWIRDLSAADPSTIWNLYGLIPWDPATAPLIGGYLTGT
                     FALSILAIFYGLTMWLQMAMSPPAPDPMQRRIFQFMPIVFTFIMATFPAGLLIYWAWS
                     NILTIIQQYVIMHRLKAENPIDTFLLRFKKA"
     gene            complement(3582..3983)
                     /gene="rnpA"
                     /locus_tag="VF686_10150"
     CDS             complement(3582..3983)
                     /gene="rnpA"
                     /locus_tag="VF686_10150"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8662216.1"
                     /translation="MAELFKIERMTSRPQFLAAAKGVSEARGAVVVQRLDRGDDLPVV
                     RLGFTATRKVGNAVIRNRAKRRLREAARAMAPLLAVPGSDYVFVARAGTADRPWDRLL
                     DDVKSALTRLATPRPAPKTAPTTPATAGQDS"
     gene            complement(4010..4144)
                     /gene="rpmH"
                     /locus_tag="VF686_10155"
     CDS             complement(4010..4144)
                     /gene="rpmH"
                     /locus_tag="VF686_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8662217.1"
                     /translation="MKRTYQPSRLVRKRRHGFRSRMATKNGQKIVARRRAKGRKRLTA
                     "
     gene            4264..4986
                     /locus_tag="VF686_10160"
     CDS             4264..4986
                     /locus_tag="VF686_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8662218.1"
                     /translation="MNRFKRFAPLIVLAGVIALIFAMGWNRYLSLDTIRDHGADFHAF
                     KDANYILCLLILMAVFAILTASVVPGVFFVTITAGYLFGPWVGGVSTSIAATVGALIV
                     YAVARSALGEALRQKAERDKGMMQKVCDAIDRDTFWYVLASRLAVVVPFHMINVAAGI
                     MAVRLGPYTIATVIGLLPAHIIYCWIGARLNQLLITNPNPDFQALFGQFWAPMAGVFV
                     LAVILPFGLKGLQKALGRKAAA"
     gene            5027..6490
                     /locus_tag="VF686_10165"
     CDS             5027..6490
                     /locus_tag="VF686_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8662219.1"
                     /translation="MNRFRPLLSDVRRALRVPADWRELLKFHAPDALAWRLLLLTFVF
                     TVVVEILIIAPSAASFHERWLLDRLQSAELASVGVEALPYSAVEDETAEQLLAIGGVQ
                     AVVVGDQGVRRLLLQAPNLPRTPDLIDLRQRRPVARTMDPWRTLFGHPDRSIRVQARP
                     RYRSGDFIEILAPAQPLKEELRAFLLNSLLMSLLISLVAGGLLYGGLAFLVLQPLRRV
                     TRSIEHFAHDPEAEGDPPSDRHDEIGRVERELARMQEEVRVSLRSRARLVALGEAVAK
                     INHDLRNMLTSAQMASERLADSPDPEVAKVLPRLERALGRAAALSRNVLEYGKSEEPP
                     PQRVRIALAPAVTAAAEDAGLTPEGVRLTKALPARFAVHADPDQLHRILVNLMRNARQ
                     AIEGDATRRTGRKGVVRVTASGDDGDCVIRIADDGPGIPPRLSESLFEPFVSGRASDG
                     AGLGLAISRELAASHGGELTLVETGPTGTTFELRLPH"
     gene            6590..7069
                     /locus_tag="VF686_10170"
     CDS             6590..7069
                     /locus_tag="VF686_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8662220.1"
                     /translation="MTRLTLAAAGALLLAAAPVLAQTTAPQTAVSAKVQTSAAPAGSE
                     EWLRQRGESYHQASDAEQNPAEVAETIRLNAEIAARNAAAERAEAEGTATFERQSAQW
                     REETARLETQRAQYEADLAASTAATAAYERARAAWEAEVAACERARRVCVTTPPAKY"
BASE COUNT         1095 a         2291 c         2513 g         1174 t
ORIGIN      
        1 gtgcgggcga aacttgcggc ctttgcggag cagacgagcg tcgacgaggt catggtcacc
       61 ggcatggtgt tcgatctcga cgaccgcatt cgcagtctgg agattacggc cgaggcggtc
      121 gcggggcttt agccgacccc gggctcacgg aggaactcca gcagcagccg gttcacctca
      181 tcgggccgtt cctgctggat ccagtggccg gcgccgggga caaccacctg cattcgcaga
      241 tcgggcgcgt gatccttaag ccccgcctcg tgctggcccg catagtggcg cacgggatcg
      301 cgttcgccga ccacgaaggc ggcggggacg cggatgcggg cgtcctgcag atgggcggtc
      361 agggaccagt tgcggtcgag gttgcggtac cagtcgagcg gccccttgaa gccgccggcc
      421 gtgaaggcct cgacatactc ggcgagatgc tccgggctca tccatggcgg caggggcgcg
      481 tcgtcgggca cggtcgagat gaagctctgg ccctcgggga tgaagcccgt cgactggcgg
      541 ccgtcggagg tggcgccgtc gtaggcgtag aacatcttgc gcagggccgt ggccggatgg
      601 gcgtcgaaga ccgcatgggc gtccgcggcc tgaaagcggc tgatgtagag gtcgccgagc
      661 cccagccgtt tcgagatcgc cgccatggcc gccgtcggcg gacccttggg ccggcggggc
      721 tggaagggca ccgacaggcc gaagacggcg ctgaacacgt cgggccgcgt cagggcgcag
      781 tgccacgcca ccggcgcgcc ccagtcgtga ccgaccacta cgcaggacgt ctcacccagc
      841 gcccgcacca gatcgaccat gtcgcccacc agatgcagga tggaatagtc cgggacgtca
      901 ttcggtttct cagttgaacc gtagccccgc atgtcgggcg cgaccgcgtg gaagcccgct
      961 tcagccaagg cctcgacctg tgcgcgccag ctgatcccca gttcggggaa cccatggatc
     1021 agcaggacca gcggcccggt tccggcctcg aggatgtgct ggttcaggcc gtctgtctgg
     1081 atgtggcgcg actgcatccg gccaccggac cactctcggg tgcggaccgc aagcccgcta
     1141 gagctctgcg cccgtggtgc gcatgatctc ggcgcggagt tcgggcatgc cgtcgccctt
     1201 ttcggccgag gtgacgatca cggccggata ggcggccggg cgtttggaga tggccttcag
     1261 cgtggcggcc tggaccttct cggcctcgcc cttcttgagc ttgtcggcct tggtgaggat
     1321 gatctggtag ctgacggcgg ccagatcgag cgcgtccagc gcctcgtcgt cgaccttctt
     1381 caggccgtgg cgggaatcga tcagcagata gacccgcttc agagtgaccc gcccgcgcag
     1441 atagtcgcgg ccaaggtcct ggaatttctt caccgtcgat ttggacgcct tggcccagcc
     1501 gtagccgggc aggtcgacca gccgcatctt gccgtcgagg tcgaagaaat tgacctcgcg
     1561 cgtgcggccc ggctcgttcg aggcccgggc cagtttgtgc attccgacca gaccgttgat
     1621 caggctggac ttgcccacgt tcgaacggcc cgcgaaggcg acctcgggca ggtcgggatc
     1681 gggcagctgc tcgatcttgg cgcaccccat gatgaaggtc gcggggcgcg cgaacaggat
     1741 gcgcccctgc tccagttcct caggggtgaa ctcgtccaac cgtcaggcct ttttgaagcg
     1801 cagcaggaag gtgtcgatcg ggttctcggc cttcaggcgg tgcatgatca catattgctg
     1861 gatgatggtc aggatgttgg accaggccca gtagatcagc aggcctgccg ggaaggtggc
     1921 catgatgaag gtgaagacga tcggcatgaa ctggaagatg cggcgctgca tgggatccgg
     1981 cgcgggcggg ctcatggcca tctgcagcca catggtcagg ccgtagaaga tggccaggat
     2041 gctgagcgcg aaggtgccgg tcagatagcc gccgatcagc ggggccgtcg cggggtccca
     2101 cgggatcagg ccgtacaggt tccagatggt cgagggatcg gccgccgaca ggtcgcggat
     2161 ccagccgaag aagggcgcgt gccgcatctc gatggtcacg aacagcatct tgtagacggc
     2221 gtagaagacc gggatctgca gcagcagcgg cagacagccc gccagcggat tgatcttctc
     2281 cttctgatac agcgccatca gttccttctg ctgggcggcc ggatcatcgg ggtgtttctt
     2341 cttcagctcc tccatcttcg gctggagggt ccgcatcctg gacatgctct catagctctt
     2401 gttggccagc gggaacatga cgaggcggac ggtcagggtc aggcacagga tggccaggcc
     2461 gaagttgccg atgatgccgt aaaagaactc gatcagcagg aagatcgggc gggtcaggaa
     2521 gaacaggaag ccccagtcga tcgcatatat gaagcgcggc aggccgaggt ccttctcgta
     2581 ggcggaaagg atctcgttgc gcttggcgcc ggcgaacagg cgctgggttt cggtgatctg
     2641 gcggccgggg gcgatggtgt gggtttcgcc cagcatggtg gccgagtgga tgtcggcatg
     2701 ggcggcgtcg gtgacgcgga aggccgcctg gatgcgctcg tcctgctgcg ggatcagggc
     2761 ggccagccag tatttgtcgg tgatgcccat ccagccgccg gtcgaggtgt gctcctgcac
     2821 cggcttcttg gcccagccct ggtatttgag ctgttcggtg gtgtaatttc cgtcggaccc
     2881 gaaggtgccg atgccgcctt cgtgcaggat ctgggtcttg cccaggccgt ccggcacgcc
     2941 ttgccgctcg acccggcccc agggcgcgat tgcgatcggc tgggcgctga agttggcgac
     3001 ggtgtcggtg acggtgaaca gatactgggc gtcgatggcg atgcggcgcg tgaagcgcag
     3061 gccggcgccg ttgtcccagg tcagggtgac aggcgtcgtc ggcgtcagcg tcgcgccctc
     3121 ggtcagacgc cagggcgtct gcggaccggg cacgccgccg ggcacattgg ggccgttcca
     3181 gccgaattgg gcctgatagg cgtggcgcat gccctcgggg cggaacagtt cgacggcggg
     3241 cgagccctcg tccatcgtct cctgatagtt ggtcaggaac agatcatcga tccgggcgcc
     3301 gcgcagcgac agcgagccag tgacggtgcc ggtcttgatc ggcacccggg ccccgccgcg
     3361 cgcgagggcg acggtgcggt cggtggtgaa ggtgacggct ccgggcgtgg tcgcgatagc
     3421 gggcgcgccg gccgtccgtt cggcgacagc ggcctgctgg gcggccttcc ggcgctcggc
     3481 ctggggctgc atcacgaaat agctgtaggc catcagcatg atggccgcga tcacgacaaa
     3541 gaggatcgtg tttcggctgt tgtcttgagg aggcatggag atcaggaatc ctggccggcg
     3601 gttgcgggcg tcgtaggcgc agtcttcggc gccggccgcg gggtcgcgag ccttgtaagc
     3661 gccgatttca catcgtcaag caaacggtcc cagggacggt cggccgtgcc cgcgcgggcg
     3721 acgaagacat agtcgctgcc ggggaccgcc aacagggggg ccatggcccg cgccgcctcc
     3781 ctgagacggc gtttggcgcg gttgcggatg acggcgttgc cgaccttgcg cgtggcggtg
     3841 aagcccagcc gcaccacggg caaatcgtcg ccccggtcca gccgctgcac gaccacggcg
     3901 ccacgggcct ctgaaacgcc cttcgcggcc gccagaaact ggggccgcga agtcatgcgt
     3961 tcgattttga agagttcggc catgcgcccg agcacaggcg ccggacgact taggccgtca
     4021 ggcgcttgcg gcccttggcg cggcggcgcg caacgatctt ctgtccgttc ttggtggcca
     4081 tccgcgaacg gaagccgtgt cggcgcttgc gcacgagtcg cgacggctga taggtccgct
     4141 tcacgttcgg ctcctgggac tgaaaacaaa tgggcggcgg aaaagcgtcc gtcgcgggaa
     4201 gggcgggcgt ataagcggca gggcctgaac tgtcaacgcc aacggccctt cggcggagag
     4261 agaatgaacc ggttcaaacg ctttgcgcct ctgatcgtac tggccggcgt catcgccctg
     4321 atcttcgcca tgggctggaa ccgctatctg tcgctggaca ccatccgcga tcacggggcg
     4381 gatttccatg ccttcaagga cgccaactac atcctgtgcc tgctgatcct gatggcggtg
     4441 ttcgccatcc tgacggccag cgtggtgccc ggcgtcttct tcgtcaccat cacggcgggc
     4501 tatctgttcg gaccgtgggt gggcggggtt tcgacctcca tcgccgccac ggtcggggcc
     4561 ctgatcgtct atgcggtggc ccgatcggcg ctcggcgagg cgctgaggca gaaggccgag
     4621 cgcgacaagg ggatgatgca gaaggtctgc gacgccatcg accgcgacac cttctggtat
     4681 gtgctggcct cgaggctggc agtggtggtg ccgttccaca tgatcaatgt ggcggcgggg
     4741 atcatggcgg tgcggctggg gccctatacg atcgcgacgg tgatcggcct gttgccggcg
     4801 cacatcatct attgctggat cggagcgcgg ctgaaccagc tgctgatcac caacccgaac
     4861 ccggactttc aggccctgtt cggccagttc tgggccccga tggcgggggt gttcgttctg
     4921 gcggtgatcc tgccgttcgg cctcaagggg ctgcagaagg cgctcggacg gaaggcggcg
     4981 gcatgaacgg cgcagctttt ggccgcagct ttgcgttaag cggcgcatga accgtttccg
     5041 gcccttgctg agcgacgtcc ggcgggccct gcgcgtgccc gccgactggc gcgagttgct
     5101 gaagttccat gcgcccgacg ccctggcctg gcggctgctg ctgctgacct tcgtcttcac
     5161 cgtagtggtg gagatcctga tcatcgcccc gagcgcagcc agcttccacg agcgctggct
     5221 gctggaccgg ctgcaatcgg cggaactggc ctcggtcggg gtggaggccc tgccctattc
     5281 ggcggtcgag gacgagaccg cggaacagct gctggccatc ggcggggtgc aggccgtggt
     5341 cgtcggcgat cagggcgtgc gccgcctgct gctgcaggcc cccaacctgc cgcgcacgcc
     5401 ggatctgatc gacctgcgcc agcggcggcc cgtggcccgc accatggatc cgtggcgcac
     5461 cctgttcggc catccggacc gctcgatccg ggttcaggcc cggccgcgct atcgctcggg
     5521 cgacttcatc gagattctgg ccccggccca gcccttgaaa gaggagttgc gggccttcct
     5581 gctgaacagc ctgctgatgt cgctgctgat ctcgctggtg gcggggggac tgctgtacgg
     5641 cggtctggcc ttcctggtgc ttcagccgtt gcggcgggtc acccggtcaa tcgagcattt
     5701 cgcccacgat ccggaggcgg agggcgatcc gcccagcgac cgccacgacg agatcggccg
     5761 ggtcgagcgc gaattggcgc gaatgcagga ggaggtgcgc gtctccctgc gctcccgcgc
     5821 acggctggtg gcgctgggcg aggcggtggc caagatcaac cacgacctgc gcaatatgct
     5881 gacgtccgct cagatggcct cggaacggtt ggccgactcc cccgacccgg aggtggcgaa
     5941 ggtcctgccc cggctcgagc gggcgctggg ccgggccgca gccctgtctc gcaatgtgct
     6001 ggagtacggc aagagcgagg agccgccgcc ccagcgggtt cgtatcgccc tggccccggc
     6061 cgtcacggcc gccgccgagg atgcaggcct gacgcccgag ggcgtgcgtc tgacaaaggc
     6121 cctgcccgcc cggttcgcgg tccacgccga tccggaccag ttgcaccgca tcctcgtcaa
     6181 cctgatgcgc aacgcccgtc aggccatcga gggcgacgcc acccggcgca ccggccgcaa
     6241 gggcgtggtg cgggtgaccg cctcgggcga cgacggcgac tgcgtcatcc ggatcgccga
     6301 cgacgggccc ggcattccgc cgcgcctgtc cgaaagcctg ttcgagccct tcgttagcgg
     6361 ccgcgcctcc gacggcgccg gcctgggtct ggccatttcg cgcgaactgg cggccagcca
     6421 tggcggggaa ttgacgctgg tggagacggg gccgacgggc accaccttcg aactgcgtct
     6481 gccgcactag cccggcggcg ccaaatcgcg cgaccggcgt tatgtgtggc ggaccgccgc
     6541 gaaaccgccc agcttcacgg caacgcttat tttcccacgg agcattcaga tgacccgcct
     6601 cactctcgcc gccgccggcg cccttctgct ggccgccgct cccgtgctgg cccagaccac
     6661 ggcgccgcag acggccgtct cggccaaggt tcagacgtcg gccgcgcccg ccgggtccga
     6721 ggaatggctg cgccagcgcg gcgaaagcta ccatcaggcc tccgacgccg aacagaaccc
     6781 ggccgaggtc gccgaaacca tccgcctgaa cgccgagatc gccgcccgca acgcggccgc
     6841 cgaacgggcc gaggccgagg gcacagcgac cttcgaacgg cagagcgccc agtggcgcga
     6901 ggagaccgcc cggctggaaa cccagcgcgc ccagtacgaa gccgatctgg cggcctccac
     6961 cgcggcgacc gcggcctatg agcgggcccg cgcggcctgg gaggccgagg tcgcggcctg
     7021 cgaacgggcc cggcgggtct gcgtcaccac cccacccgca aaatactgaa gcc
//
LOCUS       DASVVN010000623         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_129975,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000623 DASVVN010000000
VERSION     DASVVN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129975"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VF686_10175"
     CDS             complement(<1..430)
                     /locus_tag="VF686_10175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8662221.1"
                     /translation="MARAVLEPREDREIFVVPLTVLPEHIDDNGHVNNVVYVGWLQDA
                     GTAHWNARFDGETRARWSWVAVRHEIDYFRPLPPEAEGVVARTWVGDPKGPRFNRYVR
                     IEDGEGRLCAQGVSEWVLVDATTMRPHRIPQEMLPTFERRR"
     gene            complement(430..804)
                     /gene="grxD"
                     /locus_tag="VF686_10180"
     CDS             complement(430..804)
                     /gene="grxD"
                     /locus_tag="VF686_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8662222.1"
                     /translation="MTDQAVTSAAGADPVHAFIGETVKQHDVVLFMKGTPDAPRCGFS
                     SLAVQILDHIGANFVGVDVLQDEALRDGIKTFSDWPTIPQLYVKGEFVGGSDIVREMF
                     NAGELQTLMADKGVPLGEDADA"
     gene            complement(875..1798)
                     /locus_tag="VF686_10185"
     CDS             complement(875..1798)
                     /locus_tag="VF686_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8662223.1"
                     /translation="MRYRPFGVAGQAVSALTLSLGARDIAGGPEAGRELVYSALEAGI
                     NSYRLETADPVLAEVLGQALKHVDRKLVQVSLTLGAGDGRRGSDRDFSAEGITAAIDK
                     ALHASGLGWFDMALLDEPVDNELPQSTLNALKALRATERVRLLGVSGEGDVMDTYVST
                     GAFDVLATPFHVNASWQIRSRLRAAREQDMAIFVYGYFPDSLNTAKKAASAHVEKKKG
                     LFGFGGGPKVKEGPLAHAGTFAFLHRTNNWTAEAICLAYALTDPSVSSVLIEASDIER
                     LNTLSQVPDRDMPPGLAAQIEMARVAGAKAA"
     gene            complement(1803..2057)
                     /locus_tag="VF686_10190"
     CDS             complement(1803..2057)
                     /locus_tag="VF686_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8662224.1"
                     /translation="MPMPVETLRGHLTEAFPDAEIVIDDLAGDGDHYRARIVSKAFAG
                     LPRVRQHQLVYAALKGKMGGELHALALETATPPPLNLAGD"
     gene            complement(2115..2630)
                     /locus_tag="VF686_10195"
     CDS             complement(2115..2630)
                     /locus_tag="VF686_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8662225.1"
                     /translation="MTILVTERLTLTPAALSDLDDLVALWADPAFATAIFPAPLSPED
                     VWFRLLRDIGHWEALGYGNWSIRETATGAYVGSVGVLDYRRIMTPAFDAPELGWGVAP
                     RFQGKGMAFEALSAALAWCDEALNASRTVCMISPDNAPSHALAKRAGYHPYIETTYKG
                     APIVLLERTAA"
     gene            complement(2627..>2983)
                     /locus_tag="VF686_10200"
     CDS             complement(2627..>2983)
                     /locus_tag="VF686_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8662226.1"
                     /translation="KMRGRVGDSPIIGAGLYVDDEVGGATATGVGEDVVRVAGSHSVV
                     EAMRHGLDPTAACRSVIERLARLRGAKVAASQVALLALDKSGRAGAFALQPGFTYAVT
                     DAAGHTRIERAGALFP"
BASE COUNT          480 a          929 c         1102 g          472 t
ORIGIN      
        1 aacgccgacg ctcaaacgtc ggaagcattt cctgcgggat ccgatgtggc cgcatggtcg
       61 tcgcgtccac cagcacccac tcggaaaccc cctgtgcgca cagccgaccc tcgccgtcct
      121 cgatccgcac atagcggttg aagcggggcc ccttggggtc gccgacccag gtgcgggcga
      181 cgacgccttc cgcttccggg gggagggggc ggaaataatc gatctcgtgc cgcacggcca
      241 cccaggacca gcgggcccgt gtctcgccgt cgaagcgggc gttccagtgg gccgtgccgg
      301 cgtcctgcag ccagccgaca tagaccacat tgttcacatg gccgttgtcg tcgatgtgtt
      361 cgggcagcac cgtcagcggg acgacgaata tctcgcggtc ctcgcgcggt tcgaggaccg
      421 cgcgcgccat caggcgtcgg cgtcttcgcc cagcggcacg cccttgtcgg ccatcagggt
      481 ctgaagctcg ccggcgttga acatctcgcg cacgatgtca gagccgccca cgaactcgcc
      541 cttcacatag agctggggaa tggtcggcca gtcgctgaag gtcttgatgc cgtcgcgcag
      601 ggcctcgtct tgaagcacgt ctacgcccac gaaattcgcg ccgatgtggt cgaggatctg
      661 gaccgccagc gaggagaagc cgcagcgcgg cgcgtcgggc gtgcccttca tgaacaggac
      721 gacatcgtgc tgtttcaccg tctcgccgat gaaggcgtgg acgggatcgg cgccggcggc
      781 ggaagtcacg gcttggtcgg tcacgggata ctggcctctc ggtgaaggcc gtcaggtatg
      841 gaccgggcga gcgggggtca aggggcgtgc ggcgtcaggc cgccttggcg cccgccaccc
      901 gcgccatctc gatctgggcg gccaggcccg gcggcatgtc gcgatcgggc acctgggaca
      961 gggtgttgag ccgctcgatg tcgctggcct cgatcaggac gctggagacc gaagggtcgg
     1021 tcagggcgta ggccaggcag atggcctcgg cggtccagtt gttggtgcga tgcaggaagg
     1081 cgaaggtgcc ggcgtgggcc agagggcctt ccttgacctt cgggccgccg ccgaagccga
     1141 acaggccctt cttcttttcg acgtgggcgc tggccgcctt ttttgcggtg ttcaggctgt
     1201 cggggaaata gccgtagacg aagatggcca tgtcctgctc gcgggccgcg cgcaggcgcg
     1261 accggatctg ccagctggcg ttgacgtgga aaggggtggc cagaacgtcg aaggcgccgg
     1321 tcgagacata ggtgtccatc acatcgccct ccccggagac acccaacagg cggacgcgtt
     1381 cggtggcgcg cagggccttc agggcattca gggtggactg gggcagttcg ttgtcgacgg
     1441 gctcgtccag cagggccatg tcgaaccagc ccaggcccga ggcgtgcagg gccttgtcga
     1501 tggcggcggt gatgccctcg gccgagaagt cccggtcgga gccgcgtcgg ccgtcgccgg
     1561 cgccgagggt cagggagacc tgcaccagct tgcggtcgac gtgtttgagc gcctgtccca
     1621 gcacctcggc gaggacggga tcggcggtct cgaggcggta gctgttgatg ccggcttcca
     1681 gcgccgaata gacgagttcg cggccggcct cgggaccgcc ggcgatgtcg cgggcgccga
     1741 ggctgagggt gagggccgaa acggcctgtc cggcgacgcc aaaaggacgg taacgcatgg
     1801 tctcagtctc ccgccaagtt taaagggggc ggcgtggctg tttccagcgc cagcgcgtga
     1861 agttcgccgc ccatctttcc cttcagcgcg gcatagacca gctggtgctg gcgcacgcgg
     1921 ggaagtccgg cgaaggcctt ggagacgatg cgcgcacgat agtggtcgcc gtcgcccgcc
     1981 agatcgtcga tgacgatctc ggcgtcggga aaggcttcgg tcagatggcc gcgaagcgtt
     2041 tcaacaggca tgggcatgga aggcgcggtc tccggattgc gccgggatga tgcgctgtaa
     2101 accttaatgg caggctacgc agccgtgcgt tcaagcagga cgatcggcgc gcccttgtag
     2161 gtcgtttcga tataggggtg ataacccgcc cgcttcgcca aggcgtgcga cggagcgttg
     2221 tccggcgaga tcatgcagac ggttcgagac gcgttcagag cctcgtcgca ccaggcgagg
     2281 gcggcagaca gggcctcgaa ggccatgccc ttgccctgaa agcgcggggc gacaccccag
     2341 cccagttcgg gcgcgtcgaa ggcgggggtc atgatgcggc ggtagtccag cacgccgacg
     2401 ctgccgacat aggcgcccgt cgcggtctca cggatcgacc agttgccgta gcccagcgcc
     2461 tcccagtggc cgatgtcccg cagcagacgg aaccagacgt cctccgggct caggggagcg
     2521 ggaaagatgg ccgtggcgaa ggcggggtcg gcccagaggg cgacgagatc gtcgaggtcc
     2581 gacagggcgg cgggggtgag cgtcaggcgt tcggtaacga ggatggtcat ggaaacaggg
     2641 ctccggcgcg ttcgatgcga gtgtggccgg cggcgtcggt gacggcgtag gtgaagccgg
     2701 gctggagggc gaaggcgccg gctcggccgg atttgtccaa agctagaagg gcgacttgcg
     2761 aggcggcgac cttcgcgccc cgcaggcggg cgaggcgttc gatcacggaa cggcaggcgg
     2821 ccgtggggtc gaggccgtga cgcatggctt cgactaccga gtgggagccg gctacgcgca
     2881 ccacatcctc gccgacgccg gtggcggtcg cgccgccgac ctcgtcgtcg acatagagac
     2941 ccgcgccgat gatcgggctg tcgccgacgc ggccgcgcat ctt
//
LOCUS       DASVVN010000624         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_444789,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000624 DASVVN010000000
VERSION     DASVVN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444789"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..523
                     /locus_tag="VF686_10205"
     CDS             83..523
                     /locus_tag="VF686_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX8662227.1"
                     /translation="MASAFKADPAFEAGSVVVCDWPLCELRLQDDARFPWLILIPRGA
                     GLHELEDLTGAERAALMDEVVRAGDLVRALGEAAGRPVQKLNVAALGNVTAQLHLHVI
                     GRRHDDGLWPDPVWGRGAALPYGANGLQTAVSRLRLLARGAPAK"
     gene            633..1106
                     /gene="sdhC"
                     /locus_tag="VF686_10210"
     CDS             633..1106
                     /gene="sdhC"
                     /locus_tag="VF686_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX8662228.1"
                     /translation="MSISPLPGDASNDPTDDQTGRFVVQPNGRVRPLSPHLQVWRWHV
                     TMFASILNRMTGGALSVGSVLVALWLLALAFGPDAYATFTGWMGSPLGLLVWFGLTLA
                     LFLHLTGGIRHLIWDAGSGLTPKAADALSWGSMIVGVIGTVAFWAFLFASGRVVL"
     gene            1103..1510
                     /gene="sdhD"
                     /locus_tag="VF686_10215"
     CDS             1103..1510
                     /gene="sdhD"
                     /locus_tag="VF686_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX8662229.1"
                     /translation="MSRVTKAFVKGVKVSERHGAGEWTAERLSSLILVPLTLWGLWSG
                     FVLSGGGYDGAREWFASPVNAVLLAVTLLVSLWHMNMGLKVIVDDYIHKPVSRTALLG
                     LIGLLCLAIAAAGVFFIVRLALGSAPLPAGFGI"
     gene            1517..>3268
                     /gene="sdhA"
                     /locus_tag="VF686_10220"
     CDS             1517..>3268
                     /gene="sdhA"
                     /locus_tag="VF686_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX8662230.1"
                     /translation="MAAYEFVDHAYDVVVVGAGGSGLRAALGAAQQGLKVACVTKVFP
                     TRSHTVAAQGGISASLGNMGEDSWQWHMYDTVKGSDWLGDQDAIEYLVRNAPKAVYEL
                     EHWGVPFSRTDEGKIYQRAFGGMTRNFGEGPVQRTCAAADRTGHAILHTLYGQSVRRE
                     VEFFVEYFGLDLIMQDGACTGLTALQLDTGQLHRFRAKMVILATGGYGRAYVSATSAH
                     TCTGDGNAMVLRAGLPLQDMEFVQFHPTGIYGAGCLITEGARGEGGYLTNSEGERFME
                     RYAPTVKDLAPRDMVSRSMTIEIREGRGVGPNKDHINLHLDHLDPKILHERLPGISES
                     AKIFAGVDVTKQPIPVLPTVHYNMGGIPTNYHGEVLTKVGDNADTVVPGLMAVGEAAC
                     VSVHGANRLGSNSLTDLVVFGRAAGLRCGEVVSKTTRVPEAPASATDGHLARLDRFRN
                     ANGSTPTARLRIEMQRAMQSDAAVFRTGKTLEEGVAKLRAIHAAGDDIRTTDRGLIWN
                     TDLVETMEYDNLIDQALVTIEGALNRTESRGAHAREDYSERNDDIWMKHTLAWLEPKT
                     GAVRLDYRPVHAYTMSND"
BASE COUNT          485 a         1177 c         1057 g          549 t
ORIGIN      
        1 ggccgcccgg ctggcgcttc agccgccgct gcgtgaactg agcgatcggg cgtgcgtcgt
       61 ctacggcctg aactgacggc gcatggcttc tgccttcaaa gccgatcccg cctttgaggc
      121 cggttccgtc gtggtctgcg actggcccct gtgcgagctg cggcttcagg acgacgcccg
      181 gttcccgtgg ttgattctga tcccccgcgg ggccggtctg catgagctgg aggacctgac
      241 gggggccgag cgcgccgccc tgatggacga ggtcgttcgc gccggcgatc tggtgcgggc
      301 gctgggcgag gccgcgggcc ggccggtgca gaagctcaat gttgccgccc tcggcaatgt
      361 cacggcgcag ctgcaccttc atgtcatcgg ccgccggcac gacgacggcc tgtggcccga
      421 tccggtctgg ggccggggcg cggccctgcc ctatggcgcc aacgggctgc agacggcggt
      481 gtcccggctt cgcctgctgg cccgcggggc gccggcgaaa tgaagggctg tccccctcaa
      541 gcttcgttga cccccgccgc cccgctgctt aaagccagag caccgatttt gagcggctga
      601 attcgctcag gtttctgaga accattcgca atatgtcgat ctctcccctg ccgggcgacg
      661 cctcgaacga tccgaccgac gaccagaccg gccgtttcgt ggtccagccc aatggccgcg
      721 tccgtccctt gtcgccccac ctccaggtct ggcgctggca cgtcaccatg ttcgcctcga
      781 tcctgaaccg catgacgggc ggcgccctgt ccgtcggatc ggtgctggtc gccctgtggc
      841 tgctggctct ggccttcggg cccgacgcct atgccacctt caccggctgg atgggttcgc
      901 cgctgggcct gctggtctgg ttcggcctga ccctggccct gttcctgcac ctgaccggcg
      961 gcatccgcca cctgatctgg gacgcgggtt ccggcctgac gccgaaggcc gctgacgccc
     1021 tgtcgtgggg ctccatgatc gtcggcgtaa ttggaacggt cgccttctgg gccttcctgt
     1081 tcgcctccgg gagggtcgtg ctgtgagccg cgtaaccaag gccttcgtga agggcgtcaa
     1141 ggtttccgag cgccacggcg ccggcgaatg gaccgccgaa cggttgtcgt ccctgatcct
     1201 cgtccccctg accctctggg gtctttggag cggcttcgtc ctgtccggcg gcggctatga
     1261 tggcgcacgc gagtggttcg cctcgccggt caacgccgtg cttctcgccg tcaccctgct
     1321 ggtcagcctc tggcacatga acatgggcct gaaggtcatc gtcgacgact acatccacaa
     1381 gcccgtatcg cgcaccgccc tgctgggcct gatcggcctg ctgtgcctcg cgatcgcggc
     1441 ggcgggcgtc ttctttatcg tacggctggc gctgggttcc gcgcccctgc ccgccggctt
     1501 cgggatctga cggctgatgg ctgcttatga attcgtcgat cacgcctatg acgtcgtcgt
     1561 cgtcggcgcg ggcggctcgg gtctgcgtgc ggccctcggc gccgcccagc aggggctgaa
     1621 ggtcgcctgc gtcaccaagg tcttcccgac ccgctcgcac acggtcgcgg cccagggcgg
     1681 catctccgcc tcgctcggca acatgggcga ggacagctgg cagtggcaca tgtacgacac
     1741 cgtcaagggg tcggactggc tgggcgacca ggacgccatc gagtatctgg tccgcaacgc
     1801 gcccaaggcc gtctacgagc tggaacactg gggcgtgccc ttcagccgca ccgatgaagg
     1861 caagatctac cagcgcgcct tcggcggcat gacccgcaat ttcggcgagg gcccggtgca
     1921 gcgcacctgc gccgccgccg accgcaccgg ccacgccatc ctgcacaccc tgtacggcca
     1981 gtcagtccgc cgcgaggtcg agttcttcgt cgaatatttc ggcctcgacc tgatcatgca
     2041 ggacggcgcc tgcaccggcc tgacggcgct ccagcttgat acgggccagc tgcaccgctt
     2101 ccgcgccaag atggtgatcc tcgccaccgg cggttacggc cgcgcctatg tctcggccac
     2161 cagcgcccac acctgcaccg gcgacggcaa cgccatggtg ctgcgcgccg gcctgccgct
     2221 gcaggacatg gagttcgtgc agttccaccc gaccggcatc tacggcgccg gctgcctgat
     2281 caccgagggc gcccgcggcg agggcggcta tctgaccaac tccgagggcg agcgcttcat
     2341 ggaacgctat gcgccgaccg tgaaggacct cgctccccgc gacatggtca gccgctccat
     2401 gaccatcgag atccgcgaag gccgcggcgt gggtccgaac aaggaccaca tcaacctgca
     2461 cctcgaccac ctcgatccga agatcctgca cgagcgcctg ccgggcattt ccgagagcgc
     2521 caagatcttc gccggcgtcg acgtgaccaa acagccgatc ccggtcctgc cgaccgttca
     2581 ctacaatatg ggcggcatcc ccacgaacta tcacggcgag gtcctgacca aggtcggcga
     2641 caatgcggac accgtggttc ccggcctcat ggcggtgggc gaggccgcct gcgtctcggt
     2701 gcacggcgcc aaccgtctgg gctccaactc cctgaccgat ctggtggtgt tcggccgcgc
     2761 cgccggcttg cgctgcggcg aggtggtcag caagacgacc cgggtgccag aggctcccgc
     2821 ctcggccacc gacggccacc tggcccgcct cgaccgcttc cgcaacgcca acggctcgac
     2881 cccgacggcc aggctgcgaa tcgagatgca gcgcgccatg cagtccgacg ccgccgtctt
     2941 ccgcaccggc aagacgctgg aagagggcgt ggccaagctg cgcgccatcc acgccgccgg
     3001 cgacgacatc aggaccacgg atcgcggcct gatctggaac accgatctgg tcgagacgat
     3061 ggaatacgac aacctgatcg atcaggcgct ggtcaccatc gagggcgccc tgaatcgcac
     3121 ggaaagccgc ggcgcccacg cccgcgagga ctattccgag cgcaacgacg acatctggat
     3181 gaagcacacc ctggcctggc tcgagcccaa gaccggcgcg gtgaggctcg attaccgccc
     3241 ggtgcacgcc tacaccatga gcaacgac
//
LOCUS       DASVVN010000625         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_524741,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000625 DASVVN010000000
VERSION     DASVVN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524741"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF686_10225"
     CDS             complement(<1..252)
                     /locus_tag="VF686_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEX8662231.1"
                     /translation="MPEGPLLSHVIIHTDGACKGNPGPGGWGAILQTGGGHEKELWGG
                     EPLTTNNRMELMGAISALEALTRPCRVDLHTDSKYVMTGI"
     gene            320..508
                     /locus_tag="VF686_10230"
     CDS             320..508
                     /locus_tag="VF686_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662232.1"
                     /translation="MIHLKIEQVAGKPVIQLDETDLAALGAAVGDVVAVEVVEASAVE
                     RGKAFVERYIKTFEALAK"
     gene            532..897
                     /locus_tag="VF686_10235"
     CDS             532..897
                     /locus_tag="VF686_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX8662233.1"
                     /translation="MADIVTLHDYVLERTGGSSGLRDGGLLESALSRPFNRWSYEGVE
                     DFRDLAATYGIGLAKNHPFIDGNKRAAFLALGVFLEFNGWTLTATDDDAVATIYAVAS
                     SQMDEDALAVWIRDNSAAT"
     gene            991..1209
                     /locus_tag="VF686_10240"
     CDS             991..1209
                     /locus_tag="VF686_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8662234.1"
                     /translation="MGSFSIWHWAIVAIIVLLLFGGRGKLSGLMGDAAKGVKAFRDGL
                     KDSDADKPRDERAGALPRTDAEKEDLKS"
     gene            1247..1738
                     /gene="tatB"
                     /locus_tag="VF686_10245"
     CDS             1247..1738
                     /gene="tatB"
                     /locus_tag="VF686_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX8662235.1"
                     /translation="MGPGIGGGEYLVIAVVALLVVGPERLPLLLRKLGNLVAKARGMA
                     NEFRSSFDEMARQSELDELRKEVEALRTGQAMHALGADAEAAFKDIRDDLNKPLDPPM
                     LSAPDEWPDSPPVMAPLETPVADVTPKPKPTRKTPAKAKPAASIKAASGKATPVRARK
                     VDL"
BASE COUNT          308 a          561 c          599 g          296 t
ORIGIN      
        1 gatgccggtc atgacgtatt tgctgtcggt gtgcagatcg acgcggcagg gccgggtcag
       61 ggcctcaagg gcgctgatcg cccccatcag ttccatgcgg ttgttggtcg tcagcggctc
      121 gccgccccac agttcctttt cgtggccgcc gccggtctga aggatcgctc cccagccgcc
      181 gggcccgggg ttgcccttgc aggcgccgtc ggtgtggatg atgacgtggg acaagagcgg
      241 accttcaggc atggatcgcg cctccgattg atttgaggcg aatccggatt ggcgtatgtt
      301 cctaagcagg agagacgcca tgatccatct gaagatcgaa caggtcgcgg gaaagcccgt
      361 catccagctg gatgaaaccg atctggccgc tctgggcgca gcggtcggcg acgttgtcgc
      421 ggtcgaggtc gtggaagcca gtgcggtcga gcgcggcaag gcgttcgttg agcgctacat
      481 caagaccttc gaagccttgg ccaaatgacg ggcgagcccc gatggcttgc tatggccgac
      541 atcgtcacgc tgcacgacta cgtgctggag cgcaccggcg gatcgtccgg cttgcgggac
      601 ggggggctgc tggaatcggc cctgtcgcgg cctttcaacc gctggagcta tgagggcgtc
      661 gaagatttcc gcgacctcgc cgcgacctat gggatcggtc tggccaaaaa ccatccgttc
      721 atcgacggca acaagcgcgc ggccttcctc gccctgggtg tgtttctgga gttcaacggc
      781 tggacattga ccgcgacgga tgacgacgcc gtcgccacaa tctacgccgt cgcctcaagc
      841 cagatggatg aagacgccct ggcggtctgg attcgcgaca acagcgcggc gacctgacgc
      901 cgcaatcgct tggcgtcgcg ccactgaacg gcctatatgc ggcgagagat ttcgaccggg
      961 ccgacccggc ctgactgagg agagttgtcc gtgggttcat tcagcatctg gcactgggcc
     1021 atcgtcgcca tcatcgtgct tctgctgttc ggcggccggg gcaagctgtc gggcctgatg
     1081 ggcgacgccg ccaagggcgt gaaagccttt cgggacggtc tgaaggactc cgacgcggac
     1141 aagccccgcg acgaacgcgc gggcgccctg ccccgcaccg acgccgaaaa agaagacctg
     1201 aagtcgtaag ccgcatttga cgtcggagac ctgacgcatg ggtggcctgg gtcccggaat
     1261 cgggggcggc gaatatctgg tgatcgcggt cgtggccctc ctggtggtgg ggcccgagcg
     1321 cctgcccctg cttttgcgca agctgggcaa tctggtcgcc aaggcccgcg gcatggccaa
     1381 tgagttccgc tcgagcttcg acgagatggc gcgccagtcc gagctggacg agctgcgcaa
     1441 ggaggtcgag gcgctgcgca cgggtcaggc catgcacgcc ctcggcgccg atgcggaagc
     1501 ggccttcaag gacatccgcg acgatctgaa caagccgctg gacccgccca tgctgtccgc
     1561 cccggacgaa tggcccgaca gcccgccggt gatggcgccg cttgagacgc cggttgcgga
     1621 tgtcacgccg aagccgaagc cgacccggaa aactcccgcc aaggccaaac ccgccgcctc
     1681 gatcaaggcc gccagcggca aggcgacgcc cgtccgcgcc cgtaaggtcg acctgtgacc
     1741 ctggatcgcg acgagaacga gatc
//
LOCUS       DASVVN010000626         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_101962,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000626 DASVVN010000000
VERSION     DASVVN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101962"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..847)
                     /locus_tag="VF686_10250"
     CDS             complement(44..847)
                     /locus_tag="VF686_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8662236.1"
                     /translation="MEFLSSPELASQATALGQVLMIDLVLAGDNAVAVGLAAAALAPE
                     QRKKAILIGLAAAVVLRIGFALITVQLLAIIGLLLAGGVLLLWVCWKMWRELQEQRTH
                     DQAEAEAELEMALSVHHGGGPPPEALGVKRKTFGAALLQIMIADVTMSLDNVLAVAGA
                     AHEHPWIMVFGLILSIALMGVAATYIAKMLTKYRWIGYVGLAIVLFVAVRMIWEGHRD
                     VVVDLGHTAAYNETVPEFIRITPEEAAHLTGDGKGEIETEGEPGTAAAH"
     gene            complement(890..1807)
                     /gene="rlmB"
                     /locus_tag="VF686_10255"
     CDS             complement(890..1807)
                     /gene="rlmB"
                     /locus_tag="VF686_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX8662237.1"
                     /translation="MSRNPERNDRKSGKKPAFGDRSAARPSGAGAGAKGKPEGGPDRG
                     GWNPNPKPKAFSESAPRASRAKADADNFVWGRHPVVAALANPARKGMGRLLATADRAA
                     ELERDKLAHGHKIEIIEVQALDRMLPPGAVHQGLAFKVQPLAGVALEDIAGEGGVIVM
                     LDQLTDPQNVGAIFRSALAFGAKGIVVQDRHSPALAGALAKAAAGATERLPCARVTNL
                     SRALEKLADLGWRAVGLDGSAELTLEQALDNRPTVLVMGSEGDGIRRLVAEHCDVMAK
                     IPMPGGFESLNVSNAAAVALYEASRKQSV"
     gene            1954..2037
                     /locus_tag="VF686_10260"
     tRNA            1954..2037
                     /locus_tag="VF686_10260"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1988..1990,aa:Tyr,seq:gta)
     gene            2114..2187
                     /locus_tag="VF686_10265"
     tRNA            2114..2187
                     /locus_tag="VF686_10265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2146..2148,aa:Gly,seq:tcc)
     gene            2240..>2380
                     /locus_tag="VF686_10270"
     CDS             2240..>2380
                     /locus_tag="VF686_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8662238.1"
                     /translation="MAKEKFERNKPHCNIGTIGHVDHGKTTLTAAITMALAKASGGKA
                     MNY"
BASE COUNT          394 a          774 c          817 g          395 t
ORIGIN      
        1 cgagtggacc gcccgcttcg gcggagccga ggccgcctga gggctagtgg gcggccgcgg
       61 ttccgggttc gccctcggtt tcgatctcgc ccttgccgtc tccggtcagg tgcgcggcct
      121 cttcgggcgt gatgcggatg aactccggca ctgtctcgtt gtaggcggcg gtgtggccga
      181 gatcgacgac cacgtcgcgg tgaccttccc agatcatgcg cacggccacg aacagcacga
      241 tggccaggcc gacgtagccg atccagcgat acttcgtcag catcttggcg atgtaggtcg
      301 cggccacgcc catcagggcg atcgacagga tcaggccgaa gaccatgatc caggggtgtt
      361 cgtgagcggc ccccgcgacg gcgaggacgt tgtccaggga catggtgacg tcggcgatca
      421 tgatctgcag cagggcggcg ccgaaggtct tgcgcttgac gccaagggct tccggcgggg
      481 ggccgccgcc gtggtgcacg gacagcgcca tttccagctc ggcctcggcc tcggcctgat
      541 cgtgggtgcg ctgctcctgc agttcgcgcc acatcttcca gcagacccag agcagcagga
      601 cgccgccggc cagaagcagg ccgatgatgg ccagcaactg cacggtgatc agggcgaagc
      661 cgatgcgcag gacgacggcg gcggccagac cgatcaggat ggccttcttg cgctgctcgg
      721 gcgcgagggc ggcggcggcc agacccacgg ccacggcgtt gtcgccggcc agcacaaggt
      781 cgatcatcag cacctggccg agggcggtgg cctggctggc gagttcggga gacgagagaa
      841 attccatagg cgggctctag ggcaggatca gccgggcttc aatgcgggat cagacagact
      901 gtttccgcga cgcttcgtag agcgccaccg cagccgcatt tgagacgttc aggctctcga
      961 acccgccggg catgggaatc ttcgccatca cgtcgcaatg ctcggcgacc agacggcgga
     1021 tgccgtcgcc ttccgagccc atgaccagca cggtggggcg attatccagc gcctgttcca
     1081 gcgtcagctc cgctgaacca tccagcccca cggcgcgcca gcccagatcg gccagcttct
     1141 ccagcgcacg gctgagattg gtgacgcggg cgcagggcag gcgttccgtc gcgccggccg
     1201 cggccttggc cagggcgccg gcgagggcgg gggagtggcg gtcctgaacc acgatcccct
     1261 tcgccccgaa cgccagcgcc gagcggaaga tggcgccgac gttctggggg tcggtcagct
     1321 ggtccagcat gacgatgacg ccgccttcgc cggcgatatc ctccagcgcc acgcctgcaa
     1381 gcggctggac cttgaaggcc agtccctgat gcaccgcgcc ggggggcagc atgcggtcca
     1441 gcgcctggac ctcgatgatc tcgatcttgt gaccgtgggc cagcttgtcc cgctccagct
     1501 cggcggcgcg gtcggcggtc gccagcagcc ggcccatgcc cttgcgggcg ggattggcga
     1561 gggcggcgac gacgggatga cgcccccaga cgaaattgtc ggcgtcagcc ttggcgcgtg
     1621 acgcgcgcgg ggcggactca gagaaggcct tcggcttggg attggggttc cagccgccgc
     1681 ggtcgggacc gccctccggt ttgcccttgg cgcccgctcc ggccccgctt ggacgggctg
     1741 ccgaccggtc gccgaaggcc ggttttttgc ccgatttccg gtcgttgcgt tctggatttc
     1801 tcgacactat aagacgccct ccgatttgga gccctgaggg ctttcgggtc tggcgcccct
     1861 cctaaaccag gcgacgcggg acaccgagag cttttgttgt ttcgtcgacc gaaaggtcgc
     1921 cggcgcatcg gtccgacggt tcgaaaacgc gctgggggaa tgtcccgagc ggcaaagggg
     1981 ggggactgta aatcccctgc gtaagcttcg caggttcgag tcctgcttcc cccaccacgc
     2041 ggtttcgaaa cgaaggtccg gaccccatat cgggtcggct gcggaaggcc aggaagcctt
     2101 acgcagtctc cgtgcgggta tagtacaatg gtagtacagc agccttccaa gctgaatatg
     2161 tcggttcgat tccgtctacc cgctccagaa ttcttaagct gacccaatcg ccccttcccg
     2221 ggccacagga gttttggcca tggccaagga aaagttcgaa cgtaacaagc cgcactgcaa
     2281 catcggcacg atcggtcacg ttgaccacgg caagacgacg ctgacggcgg cgatcacgat
     2341 ggcgctggcc aaggcgagcg gcggcaaggc gatgaactac
//
LOCUS       DASVVN010000627         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_524792,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000627 DASVVN010000000
VERSION     DASVVN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524792"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VF686_10275"
     CDS             <1..942
                     /locus_tag="VF686_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8662239.1"
                     /translation="HAATRAVTRLSVGVAVVLIALKAFALGASGSVSILASLTDSALD
                     LVASLGVFFAVRWAAAPPDADHRHGHGKGEALAALVQAGLVFASAVFIGWEAIQRIFD
                     PRPITAGYWGVLVILISIAVTGWLVWMQGRAIKASGSMAVQADRAHYTADLAANAVVL
                     IGVISGAWLSAPGLDAAAGLVVAVWLFWGAIGMLKTAADHLVDKALPESEQIAMTTAV
                     LSDPRISGVHQLRTRMAGPVVMIQMHVDLDPDLTLEAAHEIVVEAENRLLAGWPRADI
                     LIHPDPRGRTRPHGGVFAETADEPSTPSPKAKKGPWT"
     gene            990..1673
                     /locus_tag="VF686_10280"
     CDS             990..1673
                     /locus_tag="VF686_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8662240.1"
                     /translation="MSACVLYHFAFDPGSRAVRLALGEAKKPFTETLVRPWEDDCPVA
                     GMNPSGMPPVLQVTEDSRSLTLCEPAAILGWLEDSSRDPFLMPTDPAERAETRRLIGW
                     FDRRFTDEVNAVLLHERMEKPLLRLGSPDARALRAGREALRSHLAMLEDLLASRDALS
                     GRRIGQADFIAAAHLSVLDYFGEVPWANWPGLKTWYMKLKSRPSFRPLLADRFPGVAG
                     SAWYADLDF"
     gene            1698..2735
                     /gene="queG"
                     /locus_tag="VF686_10285"
     CDS             1698..2735
                     /gene="queG"
                     /locus_tag="VF686_10285"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX8662241.1"
                     /translation="MPVDVRPFIRERAAALGFDACRFASASAPWSAGERLAHFVEAGR
                     HGDMGWMETTLERRAHPTAMWGGARAAIVLGLNDGPETDPLPEMADRSAGDGSVYARG
                     DDYHEVIKGRLKTLAGQIAARTGEDVKVFVDTAPLMEKPLAQRAGLGWQGKHTNLLSR
                     THGNWLFLGVILSAAELEPDPPEGEHCGTCTACLDACPTNAFPAPFQLDARRCLSYLT
                     IEFAGPWPEEFRTATGSRIYGCDDCLAVCPWNKFAAASNEARVQAREALVSPRLADLA
                     ALDDAAFRTLFSKSPVKRIGRDRFLRNVLYAIGNSADPELIPSAERLLDDPDPVVRDA
                     AEWAVRRLRAD"
     gene            complement(2732..3274)
                     /locus_tag="VF686_10290"
     CDS             complement(2732..3274)
                     /locus_tag="VF686_10290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8662242.1"
                     /translation="MRYGWVRGVAALSMMAALAGPLAACNREPAAPDESAAQNLRAAE
                     FFLSSNAKAEGVKTLPSGVQYKIVRSGPPGGETPDRNDLVRVDYEGTLTDGTVFDSSF
                     QSGQPAVFTVGDVVPGWTEALQHMRVGDEWVVYVPPAQGYGEQGRPGIPPNAVMVFRI
                     KLLDIARAPGGERGGPIAQG"
     gene            complement(3255..4457)
                     /locus_tag="VF686_10295"
     CDS             complement(3255..4457)
                     /locus_tag="VF686_10295"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX8662243.1"
                     /translation="MTVTFDDILAAQKRIAGQVDRTPVRYSRRLSQLTGAEVWVKFDN
                     LHFTGSFKERGALNRLLQLTPDERKRGVVAASAGNHAQALAYHGARLGVPVTIVMPEG
                     TPFVKADGTRAHGATVVIKGQDFTGSTEEAHRLRDTEGFVFVSAFDDEGIIAGQGVCG
                     IEFMEDAPDLDVLIIPIGGGGLIAGTAIAAKAMKPSIRIFGVEAAMYPSYTARREGRP
                     PVCSGQTIAEGIAIKAVGEIPFVLVDPLIEDVLVVGEADFETGVAYLATLEKTVAEGA
                     GAGGLAALLAYPDKFRGMKVGIELTGGNIDTRMLAVVLNRELVREKKMIVYRILGDDR
                     PGMLSKMSAVIGGLGGNIIDVVHNRLALDVPAKGAEFDIMVETRGAAHAMEIRHGLEE
                     AGYALRMG"
     gene            4597..5112
                     /locus_tag="VF686_10300"
     CDS             4597..5112
                     /locus_tag="VF686_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662244.1"
                     /translation="MSARHLIAIAAVAASLAACETVPSGGFGPVPGSGPSSSAFRAQD
                     FAWSARSGRASIDGRVDYRRDGQTFHCTGTVALTPDTPYTRARFQTLYGSTTRAAIPE
                     AVVRARTVADPNADYRSFVRQTSCENNRFQFDNLPDGNWFVISPVSAGGDRIVLMRSV
                     ATRGGRVGVTL"
BASE COUNT          729 a         1977 c         1649 g          850 t
ORIGIN      
        1 catgccgcga cccgcgccgt cacccggctg tcggtcggcg tggccgtggt cctgatcgcg
       61 ctgaaggcgt tcgcgctcgg ggcctcgggc tcggtgtcga tcctcgcctc gctgacggat
      121 tcagccctcg atctggtcgc ctcgctgggc gtcttcttcg ccgtgcgctg ggccgccgcc
      181 ccgcccgatg cggatcaccg ccacggccac ggcaagggcg aggccctcgc tgccctggtg
      241 caggcgggtc tggtcttcgc ctcggccgtc ttcatcggct gggaggccat ccagcgcatc
      301 ttcgaccccc gtccgatcac cgccggctac tggggcgtgc tggtcatcct gatctccatc
      361 gccgtcacgg gctggctggt ctggatgcag ggccgcgcca tcaaggccag cggctccatg
      421 gcggttcagg ccgaccgcgc ccactacacc gccgatctgg cggccaatgc cgtagtcctg
      481 atcggcgtca tctccggcgc ctggctgtcc gcccccggtc tggacgccgc cgctggtctg
      541 gtggtcgcgg tctggctgtt ctggggcgcg atcgggatgc tgaagacggc cgccgatcat
      601 cttgtcgaca aggccctgcc cgagagcgag cagatcgcca tgaccaccgc ggtcctgtcg
      661 gatccccgga tttccggcgt gcatcagctg cgcacccgca tggccggccc ggtcgtcatg
      721 atccagatgc acgtcgatct cgatcccgac ctgacgctgg aagccgccca cgagatcgtg
      781 gtggaggccg agaaccggct gctggccggc tggccccggg ccgacatcct gattcatccg
      841 gacccccgcg gccggacccg accccacggg ggcgtcttcg ccgaaactgc ggacgaaccc
      901 tccacgccct cgccgaaagc caaaaaaggt ccgtggacct gagggcgtgg ctaacgcggg
      961 cttaacgccc tcacgcgcat gaacgggcca tgtcggcctg cgtcctgtat catttcgcct
     1021 tcgatccggg ctcgcgcgcc gtgcgtctcg cgctgggcga ggccaaaaag cccttcaccg
     1081 agacgctggt ccgcccttgg gaggacgatt gcccggtcgc cggcatgaac ccgtcgggca
     1141 tgccaccggt gcttcaggtc accgaggaca gccgctccct gaccctgtgc gaaccggccg
     1201 ccatcctcgg ctggctcgag gacagttcgc gcgatccctt cctgatgccg accgatcccg
     1261 ccgagcgcgc cgagacgcgc cgcctgatcg gctggttcga ccgccgtttc accgacgagg
     1321 tcaacgccgt cctgctgcac gagcggatgg agaagcccct gttgcgtctc gggtcgcccg
     1381 acgcccgcgc cctgcgcgcc gggcgagagg ccctgcgatc gcatctcgcc atgctggaag
     1441 acctgctagc cagccgtgac gccctgtccg gacgtcgcat cggtcaggcc gacttcatcg
     1501 cggccgccca tctatccgtg ctcgactatt tcggcgaggt cccctgggcc aactggccgg
     1561 gcctgaagac ctggtacatg aagctgaaat cccgccccag cttccgcccc ctgctggccg
     1621 accgttttcc gggcgtcgcc ggctcggcct ggtacgccga tctcgacttc taggggccgg
     1681 cgcgtcaaac gacgcccatg ccggtcgatg tccgtccctt catccgcgag cgcgccgccg
     1741 ccctgggctt cgacgcctgc cgcttcgcct cggcctcggc gccgtggagc gcgggcgagc
     1801 ggctggcgca tttcgtcgag gccggccgcc atggcgatat gggctggatg gagaccacgc
     1861 tcgagcgccg cgcccacccc accgccatgt ggggcggcgc ccgcgccgcc atcgtcctgg
     1921 gcctcaacga cggcccggag accgatcccc tgcccgaaat ggccgaccgg tcggccgggg
     1981 atggctcggt ctacgcccgc ggagacgact atcacgaggt catcaagggc cggttgaaga
     2041 ccctcgccgg ccagatcgcc gcgcgcaccg gcgaggatgt gaaggtcttc gtcgacaccg
     2101 cccccctgat ggaaaagccg ctggcccagc gcgccggcct cggctggcag ggcaaacaca
     2161 ctaatctgct cagccgcacg catggcaact ggctgttcct cggcgtcatc ctgagcgccg
     2221 ccgagcttga gcccgatccg cccgagggcg agcattgcgg gacctgcacc gcctgtctcg
     2281 acgcctgccc gaccaacgcc ttccccgccc ccttccagct cgacgcccgc cgctgcctgt
     2341 cctacctgac catcgaattc gcggggcctt ggccggaaga gttccgcact gcgacaggca
     2401 gccggatcta cggctgcgac gactgtctgg cggtctgtcc gtggaacaag ttcgccgccg
     2461 cctcgaacga ggcgcgggtg caggcgcgcg aggcgctggt ctcgccccgt ctcgccgatc
     2521 tggcggcgct ggacgacgcg gccttccgaa ccctgttctc gaagagcccg gtcaagcgga
     2581 tcggccgcga ccggttcctc cgcaacgtcc tgtacgccat cggcaacagc gccgacccgg
     2641 agctgatccc gtcggccgaa cgcctgctgg acgaccctga ccccgtggtg cgcgacgcgg
     2701 cggagtgggc ggttcggcgc ctccgggccg actagccctg cgcgatcggc ccgccccgtt
     2761 cgccgccggg cgcccgggcg atgtccagca gcttgatgcg gaacaccatc accgcattgg
     2821 gcggaatacc cggccgtccc tgctcgccat aaccctgcgc cggcggcaca tagaccaccc
     2881 attcgtcgcc gacgcgcatg tgctgcagcg cctccgtcca gcccggcacc acgtccccga
     2941 ccgtgaacac cgctggctga cccgactgga acgagctgtc gaacactgtc ccgtcggtca
     3001 gggtgccctc atagtcaacg cggaccagat cgttgcggtc gggcgtctcg ccgccgggcg
     3061 gacccgagcg gacgatcttg tactgcacgc ccgacggcag ggtcttcacc ccctcggcct
     3121 tcgcattcga gctcagaaag aactcggcgg cccgcaggtt ctgcgccgcg ctttcgtccg
     3181 gcgcggccgg ttcgcggttg caggcggcca gaggccccgc cagcgccgcc atcatcgaca
     3241 gcgccgcgac gcccctaacc catccgtagc gcatagcctg cctcttccag tccgtgtctg
     3301 atttccatgg catgggccgc gccgcgggtc tcgaccatga tgtcgaactc cgcgcccttg
     3361 gccggcacgt ccagcgccag ccggttgtgc accacgtcga tgatgttgcc gcccagaccg
     3421 ccgatgacgg cgctcatctt ggacagcatg cccggtcggt cgtcgcccag gatgcgatag
     3481 acgatcatct tcttctcgcg cacgagctcg cggttcagga ccacggccag catgcgggtg
     3541 tcgatgttgc cgccggtcag ttcgatcccg accttcatcc cccggaattt gtcgggatac
     3601 gccagcaatg cggccagccc gccggccccg gcgccctcgg ccaccgtctt ctccagcgtg
     3661 gccagatagg cgacccccgt ctcgaaatcc gcctctccga cgaccagcac atcctcgatc
     3721 agcggatcga ccaggacgaa cggtatttcg cccacggcct tgatggcgat gccctcggcg
     3781 atggtctggc cgctgcacac gggcggccgg ccttcgcgcc gcgccgtata ggacgggtac
     3841 atggccgcct cgacgccgaa gatgcggatc gagggcttca tcgccttggc cgcgatggcc
     3901 gtgccggcga tcagtccgcc gccgccgatc ggaatgatca gcacgtcgag atcgggcgcg
     3961 tcctccatga actcgatccc gcagacgccc tggcctgcga tgatgccctc gtcgtcgaag
     4021 gccgagacaa agacgaatcc ctcggtgtcg cgcagccggt gcgcctcttc ggtcgagccg
     4081 gtgaagtcct gacccttgat gaccacggtc gcgccgtgcg cccgggtgcc gtccgccttc
     4141 acgaaggggg tgccctcggg catgacgatg gtcaccggca cgcccagccg ggccccgtga
     4201 taggccagcg cctgagcgtg attgccggcc gaggccgcca ccacgccgcg cttgcgctcg
     4261 tccggcgtca attgcagcag ccggttcagg gccccgcgct ccttgaagct gccggtgaaa
     4321 tgcagattgt cgaacttgac ccagacctcg gcgccggtca gctgcgacag ccgccgcgaa
     4381 tagcggaccg gggtccggtc cacctggccg gcgatgcgct tctgcgccgc cagaatgtcg
     4441 tcaaacgtga ctgtcatgag cggtccctat accgggtccg cgcccggttg atgcgcgctt
     4501 ttggcccaca gcccgacgcc tcgcaacctt gaccttatga cggcgttcag cgtctgatcc
     4561 cagacgttcg acccgcgccc aagacggagt agagccatgt ccgcccgcca cctgatcgcc
     4621 atcgccgccg tggccgcctc gctggcggcg tgcgagaccg tcccctcggg cggttttggc
     4681 cccgtgcccg gctctggccc gtcgtcgagc gccttccggg cccaggactt cgcctggtcc
     4741 gcccggtcgg gccgcgcctc gatcgacggc cgcgtcgact atcgccggga cggccagacc
     4801 ttccactgca caggcacggt cgccctgacg cccgataccc cctacacccg cgcccggttc
     4861 cagaccctgt acggctcgac cacccgggcc gcgatccccg aggccgtggt tcgcgcgcgc
     4921 accgtggccg accctaacgc cgactaccgc tcgttcgtcc gccagacctc ctgcgagaac
     4981 aaccgcttcc agttcgacaa tctgcccgac ggcaactggt tcgtgatctc cccggtcagc
     5041 gccggcggcg accgcatcgt cctgatgcgc agcgtggcca cgcgcggcgg ccgggtcggc
     5101 gtcaccctct agcctgagaa caccagtgcc tgtcgtcttt gaggccgcgt aactggccgc
     5161 ttcggagagt taaccatgcg ccgcttcgcc ctgctcctcg ccgcc
//
LOCUS       DASVVN010000628         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_50083, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000628 DASVVN010000000
VERSION     DASVVN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50083"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..202
                     /locus_tag="VF686_10305"
     tRNA            128..202
                     /locus_tag="VF686_10305"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:160..162,aa:Asn,seq:gtt)
     gene            complement(342..1091)
                     /locus_tag="VF686_10310"
     CDS             complement(342..1091)
                     /locus_tag="VF686_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662245.1"
                     /translation="MKRAIFGKAAMAVLLAALAAACAPPVAPERVAEVESAAAGWTRP
                     PEIHAVQRASASLIFTGAAQPGARVVLRSDSGAAYAAAADGRGRFEIRMTAPAGDLWL
                     RPETQVGQDSAPSPDRLLIVAGGRGPIAILRTGGPTRRLDRAPALGAVDSDGRMRLAS
                     GRAAGTTPVVVQAAGESVRVAPDAEGRWSLMLRPADGPDEIQAGAGRFVWPGEAGGGT
                     APRVERAGQGWRVVWTGSGGARQSTWLPDAG"
     gene            1173..1772
                     /locus_tag="VF686_10315"
     CDS             1173..1772
                     /locus_tag="VF686_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX8662246.1"
                     /translation="MSSPVIQPFDGKSVCLFCGSSDASDPAYTDAARAFGEQTAREGW
                     RLVYGGGGVGLMGASARGAHAAGGRVLGVMPGFLRSRERLFDEVETIVVPSMHERKTI
                     MYDQSDAFVVAPGGVGTLEEVIEVLSWKRLDLHSKPVIFLNLNGFWEPLLAVMEHSIE
                     SGMTPATFRQAWVVCDTVEAAIEAMKGADAMPHLKHDQR"
     gene            1900..2190
                     /locus_tag="VF686_10320"
     CDS             1900..2190
                     /locus_tag="VF686_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662247.1"
                     /translation="MRALLIAAALFVAAPAVAQAPVSSAVLADASKAPASATIIDGAS
                     WRCTGGSCTASGGANQPAPRACRRVVARFGAVSSFSYKGVALSAEQLAVCNA"
     gene            complement(2264..3598)
                     /gene="gltX"
                     /locus_tag="VF686_10325"
     CDS             complement(2264..3598)
                     /gene="gltX"
                     /locus_tag="VF686_10325"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8662248.1"
                     /translation="MSVKVRFAPSPTGKLHVGNVRTALVNWMFAKGQGGSFVLRIDDT
                     DLARSTPEFEQGIEDDLTWLGLVWDERHNQSKRFDRYEDAAARLKAAGRLYACYDTSE
                     ELDRRRKVQLSRGLPPIYDRAGLALTDAERAAYEAEGRKPHWRFRLDGRRVAWEDLSR
                     GHAEVDTASMSDPVLIREDGLFLYTLPSVVDDIDMAITHVIRGEDHVTNTGAQIEIFE
                     ALGGPVPAFAHMPLLVGEDGQALSKRLGSLSIAEMRDKGYEPIAITSHLGRIGTSDPL
                     EVGESIDALGSSFAFAKMGRSPARYDTADLDRLNAQALHAMPYAVAQPRLAELDADLG
                     ETFWNGVRGNLQKFADVTEMARMVRGPVTPTIEDPAFAQAALTALPEVIDETLWPVWT
                     AAVKEATGAKGKALFMPLRLMITGQSHGPDMATLAPMIGRERIVKRLNGETA"
     gene            complement(3666..>4007)
                     /gene="dksA"
                     /locus_tag="VF686_10330"
     CDS             complement(3666..>4007)
                     /gene="dksA"
                     /locus_tag="VF686_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX8662249.1"
                     /translation="FKNKLLQWKDDILRESRETLSHLQRETENHPDITDRASSETDRA
                     LELRTRDRQRKLISKIEDALRRIEDGTYGYCEDTGEPIGLGRLEARPTATLSVEAQER
                     HERRERVHRDD"
BASE COUNT          594 a         1407 c         1329 g          677 t
ORIGIN      
        1 gtcgcggaag cccgcgatgc gcggatcatg ggggtcgtcg atgcggatca cggccaagat
       61 cggatatttc ccgttgcgtc actctgactc gcgctgctat agcgcccgcc ttcccgagag
      121 gaagtgttcc gcggtagctc agtggtagag cagccgactg ttaatcggct ggtcgtaggt
      181 tcgaatccta cccgcggagc cactctttcc caccacagcg tcagaccgaa aaaaagcccg
      241 acgcgagcgc cgggctgaag aagttcggtg atggtgggtg tcctcgaaga aggaagacat
      301 ccttccgttg aagcgaattg gttaatcgga cgtaaaccgt gtcagccggc gtcaggcagc
      361 caggtcgact ggcgcgcgcc gccggaaccg gtccagacca cgcgccagcc ctgcccggcc
      421 cgctcgaccc gcggcgcggt cccgccgccc gcctcgccgg gccagacgaa acgcccggcg
      481 cccgcctgga tttcatccgg accgtcagcc ggtctcagca tcaggctcca gcgaccttcg
      541 gcgtccggcg cgacgcgcac ggattcgccg gccgcctgca ccacgaccgg cgtcgttccc
      601 gcggcccggc cagacgccag ccgcatgcgg ccgtcgctgt cgaccgcgcc cagcgccggc
      661 gcccggtcca gacgccgggt cgggccgccg gtccgcagga tggctatggg tccccgcccc
      721 ccggccacga tcagcagccg gtcgggagac ggggcggagt cctggccgac ctgggtttcg
      781 ggccgcagcc acagatcccc cgccggcgcc gtcatgcgaa tctcgaaccg gccgcgcccg
      841 tccgccgccg ccgcataggc ggcgcccgaa tcgctgcgca gcaccacgcg ggctcccggc
      901 tgggccgcgc cggtgaagat caggctggcc gaggcccgct gcacggcgtg gatttccggc
      961 ggccgggtcc agcccgccgc cgcgctctcg acctcggcga cgcgctcggg cgccacgggc
     1021 ggagcgcagg cggcggccag cgccgccaga agcaccgcca tcgccgcctt gccgaatatg
     1081 gcccgtttca tcgcgtcgat ccgaccgcta ggagtattcc cccagatagc gcgcccgggg
     1141 ggccgctgtc tcccatccgt tcccgaggcc ccatgtcttc gcctgtcatc cagcccttcg
     1201 acggaaagtc cgtctgcctg ttctgcggtt cgtccgacgc atccgatccc gcctacaccg
     1261 acgccgcgcg cgcgtttggc gaacagacgg cgcgtgaagg ctggcggctg gtctacggcg
     1321 gcggcggcgt cggccttatg ggcgcctcgg cgcgcggggc ccatgcggcc ggcggacggg
     1381 tccttggcgt catgccgggc ttcctgcgca gccgggagcg actgttcgac gaggtcgaga
     1441 ccatcgtcgt gccttcgatg cacgagcgaa agacgatcat gtacgatcag tcggacgcct
     1501 tcgtggtcgc cccgggcggc gtcggcacgc tcgaggaagt cattgaggtg ctgtcgtgga
     1561 aacggctgga cctgcattcc aagccggtca tcttcttgaa tctcaacggc ttctgggaac
     1621 cgttgctggc cgtcatggag catagcatcg agtccggcat gaccccggcc accttccgtc
     1681 aggcctgggt cgtctgcgac acggtcgagg ccgcaatcga ggccatgaag ggcgcggacg
     1741 ccatgccgca cttgaaacat gatcagcgtt aagtaatcac tggtcagtca gccggtgttt
     1801 atgcgtttct tttgaagacg caccttgaca gtttcgtata aaaacggaca ctggcgctgc
     1861 gggaacgatg gtcgtttccg cctgtccgga gacctcccca tgcgtgctct gcttatcgcc
     1921 gcggcgctgt tcgtcgccgc tcccgccgtc gcccaggctc cggtctcgag cgcggttctg
     1981 gccgacgcct ccaaggcgcc cgcctccgcc accatcatcg acggcgcctc gtggcgctgc
     2041 accggcggtt cctgcaccgc cagcggcggc gcgaaccagc ccgccccgcg cgcctgccgc
     2101 cgcgtcgtcg cccgcttcgg cgctgtgtcc tcgttcagct acaagggcgt cgccctgtcg
     2161 gccgaacaac tggccgtctg caacgcctga tccggaagaa cggaaacgga aaaggccgcc
     2221 ctgcgagggc ggcctttttg ttggtccagc ggctggttga actctaggcg gtttcgccgt
     2281 tcagccgctt cacgatccgc tcccggccga tcatcggcgc cagcgtcgcc atgtccggcc
     2341 cgtgcgactg gccggtgatc atcaggcgca ggggcatgaa cagcgccttg cccttggccc
     2401 ccgtggcctc cttcaccgcc gccgtccaca ccggccacag ggtctcgtcg atgacctccg
     2461 gaagggccgt gagcgcggct tgggcgaagg ccgggtcttc gatcgtcggc gtgaccggac
     2521 cgcgcaccat cctcgccatt tcagtgacat cggcaaactt ctgaaggttg cctcgcacgc
     2581 cgttccagaa ggtttcgcca agatcggcgt ccagttcggc cagccgcggc tgcgccacgg
     2641 cgtacggcat ggcgtgcagg gcctgagcgt tcaggcggtc cagatcggcg gtgtcgtagc
     2701 gcgccggcga ccggcccatc ttggcgaagg cgaaactgga cccgagggcg tcgatggact
     2761 cccccacctc cagcggatcg ctggtgccga tgcggcccag atggctggtg atggcgatgg
     2821 gctcatagcc cttgtcccgc atctcggcga tcgacagcga gcccagccgt ttcgacagcg
     2881 cctgcccgtc ctcgccgacc agcaggggca tgtgggcgaa cgccggaacc ggtccgccca
     2941 gcgcttcgaa gatctcgatc tgggcgccgg tgttggtgac gtggtcctcg ccgcggatca
     3001 catgggtgat ggccatgtcg atgtcgtcga cgaccgacgg cagggtgtag aggaacaggc
     3061 cgtcctcgcg gatcagcacc ggatccgaca tcgaggccgt atcgacctcg gcatggccgc
     3121 gcgacaggtc ttcccaggcg acgcgccgtc cgtccagcct gaagcgccag tgcggcttgc
     3181 gcccctcggc ctcataggcg gctctttccg cgtcagtcag cgccagaccg gcgcggtcgt
     3241 agatcggcgg caggccgcgc gacagttgca ccttgcgacg gcggtccagt tcctcggagg
     3301 tgtcgtagca ggcgtacagc cgcccggccg ccttcaggcg cgccgcggcg tcctcatagc
     3361 ggtcgaaccg cttcgactgg ttgtgacgtt cgtcccagac cagacccagc caggtcaggt
     3421 cgtcctcaat gccctgctcg aactctggcg tggaccgcgc cagatcggtg tcgtcgatgc
     3481 gcaggacgaa actgccgccc tgccctttcg cgaacatcca gttcacgaga gccgtgcgga
     3541 cgttgccgac gtgcagctta cccgtcggcg acggggcgaa acggaccttg acggacatgg
     3601 agcgaccttg aaaacgaggc gcgtaggtcg cgcccccgtc ccttcaagtc aaggccgacg
     3661 ggcgatcagt cgtcccggtg gacgcgttcg cggcgctcgt gacgttcctg ggcctcgacc
     3721 gacagggtcg cggtcggacg ggcttccaga cggccgaggc cgatgggttc gccggtatcc
     3781 tcgcaatagc cgtaagtgcc gtcctcgatc cgacgcagcg cgtcctcgat cttggagatc
     3841 agcttgcgct ggcggtcgcg ggtgcggagc tccagcgccc ggtcggtctc cgaggacgcc
     3901 cggtcggtga tgtcggggtg gttctcggtc tcccgctgca gatgggacag ggtctcccgc
     3961 gattcacgca ggatgtcgtc tttccactgc aacagcttgt ttttgaa
//
LOCUS       DASVVN010000629         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_340010,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000629 DASVVN010000000
VERSION     DASVVN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340010"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..594
                     /gene="ruvC"
                     /locus_tag="VF686_10335"
     CDS             64..594
                     /gene="ruvC"
                     /locus_tag="VF686_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX8662250.1"
                     /translation="MTNALIPGGATRILGLDPGLRRTGWGVVVAEGSRLRWVAHGVVT
                     PPEAAPFSERLLFLLEAVGEVCASHGCDEAAIEEVFVNMNPSSTLKLGHARAAVMLAP
                     ARCGLPVAEYSPNLIKKAVVGAGHADKSQIAFMVKRLLPSAGDVKADAADALAVAITH
                     AQLRKRALLTTLRGAA"
     gene            591..1208
                     /gene="ruvA"
                     /locus_tag="VF686_10340"
     CDS             591..1208
                     /gene="ruvA"
                     /locus_tag="VF686_10340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8662251.1"
                     /translation="MIGRLRGLLAEVEQDHCLIDCAGVGYVVACGARTLQRLPAPGDE
                     ATLHIESSWSAENGPRLYGFLTRDERRAFTTLTGIQGVGPKAALSVLDVLPPGELAAA
                     VARDDKAAVARANGVGPKLALRIVTELKGKPLGDVSFTPSAAGMHAEVAAAVPSVTGE
                     AVSALLGLGVAEINARRAVDQALIRLGEDAELSAVIRAALQELGR"
     gene            1205..>1734
                     /locus_tag="VF686_10345"
     CDS             1205..>1734
                     /locus_tag="VF686_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8662252.1"
                     /translation="MTDDRIVSGAPVPGEVHDRALRPQTLSEFVGQEQAKGNLKVFID
                     AARGRGEALDHVLLFGPPGLGKTTLAQIVARELGVGFRATSGPILAKAGDLAAILTNL
                     EPRDVLFIDEIHRLAPTVEEILYPAMEDHVLDLIIGEGPSARSVRIDLAPFTLVAATT
                     RAGLLATPLRDRFGIPL"
BASE COUNT          238 a          605 c          626 g          265 t
ORIGIN      
        1 caataaccag cctcgcccgc cggtcattgt ccatcaacct tctttgcggc atgaagggaa
       61 cgaatgacga acgctttgat tcctggcggg gcgactcgca tcctcggact ggaccccggg
      121 cttcgccgca cgggttgggg cgtcgtcgtg gccgaggggt cgcgtctgcg ctgggtcgcg
      181 cacggggtgg tcaccccgcc tgaggccgcg ccgttcagcg aacggctgct gtttctgctc
      241 gaggccgtgg gcgaggtctg cgcctcacac ggctgcgatg aggcggccat cgaggaggtg
      301 ttcgtcaata tgaacccgtc gtcgaccctg aaactgggcc acgcccgcgc ggccgtcatg
      361 ctggcccccg cgcgttgcgg cctgccggtg gcggaatatt cgccgaacct gatcaagaag
      421 gccgtggtcg gcgccgggca cgccgacaag agccagatcg ccttcatggt caaacgcctg
      481 ctgccctcgg cgggcgacgt gaaggccgat gcggccgatg cgctggcggt ggccatcacc
      541 cacgcccagc tgcgcaagcg ggccctgctg acgaccctgc ggggcgccgc gtgatcgggc
      601 gtctcagagg cttgctggcc gaggtcgagc aggatcactg cctgatcgac tgcgccggcg
      661 tcggctatgt cgtggcctgc ggggcgcgca ccctgcagcg gctgccggcg cccggcgacg
      721 aggcgaccct gcatatcgaa tccagctggt cggccgagaa cgggccgcgg ctgtacggct
      781 tcctgacccg cgacgagcgc cgcgccttca ccaccctgac gggcatccag ggcgtgggcc
      841 ccaaggcggc cctgtcggtg ctcgacgtcc tgccgcccgg cgagctggcg gcggcggtgg
      901 cgcgcgacga caaggctgcg gtcgcccgcg ccaatggcgt cggacccaag ctggcgctgc
      961 gcatcgtcac cgagctgaag ggcaagccgc tgggcgacgt gtcgttcacg ccgagcgcgg
     1021 ccggcatgca cgccgaggtc gcggctgcgg ttcccagcgt cacgggcgag gcggtgtcgg
     1081 ccctgctcgg tctgggcgtg gccgagatca acgcccgccg cgccgtcgat caggcgctga
     1141 tccgtctggg cgaggacgcc gaactgtcgg cggtgatacg cgcggcgctg caggagctgg
     1201 ggcggtgacc gacgaccgca tcgtgtccgg cgcgcccgtc cccggcgagg ttcacgaccg
     1261 cgccctgcgg ccccagaccc tgtccgaatt cgtcggtcag gagcaggcca agggcaattt
     1321 gaaggtcttc atcgacgccg cgcgcggccg gggcgaggcg ctggaccacg tcctgctgtt
     1381 cggcccgccg ggactcggca agaccacgct ggcgcagatc gtggcgcgcg aactgggcgt
     1441 cggcttccgc gccacctcgg ggccgatcct ggccaaggcg ggcgatctgg ccgccatact
     1501 gaccaatctc gaaccgcgcg acgtgctgtt catcgacgag atccaccgcc tcgccccgac
     1561 ggtcgaggag atcctgtatc cggccatgga ggaccatgta ctggacctga tcatcggcga
     1621 ggggccttcc gcgcggtcgg tgcggatcga tctggcgccc ttcaccctgg tcgcggcgac
     1681 gacgcgggcg ggcctgctgg cgacaccgct gcgcgaccgt ttcggcattc cgct
//
LOCUS       DASVVN010000630         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_340219,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000630 DASVVN010000000
VERSION     DASVVN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340219"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF686_10350"
     CDS             complement(<1..379)
                     /locus_tag="VF686_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX8662253.1"
                     /translation="MTVEAARETAKRFVTLPARPEPIRIAVEETAIVVIDMQNAYASK
                     GGYVDEAGFDIAGAAGTIGQIAKVLETARGAGVTVVYLQNGWDADYVEAGGPGSPNWH
                     KSNALKTMRARPELQGRLLARGGW"
     gene            complement(504..1085)
                     /locus_tag="VF686_10355"
     CDS             complement(504..1085)
                     /locus_tag="VF686_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX8662254.1"
                     /translation="MSRTADHPIDPLFLERWSPRAFDGAAMAQEELLAMFEAARWAPS
                     AFNGQPWRFLYAHRGGADWERFLGLLIPWNQAWAQSASVLVFILSDSLVETKSGGVAA
                     SHSHSFDAGAAWASMALQATRMGWFAHGMSGVQFERARDELGVPERFRIEAAVAIGRI
                     GDPATLPEKLRAREVPSGRKRVEEFAFQGGFPK"
BASE COUNT          231 a          610 c          457 g          241 t
ORIGIN      
        1 cccagccgcc acgggcgagc aaccgtcctt gcagctcggg ccgcgcccgc atcgtcttca
       61 gcgcattgga cttgtgccag ttgggcgatc ccggcccgcc cgcctcgaca tagtccgcgt
      121 cccacccgtt ctgcagatag accaccgtca cgcccgcgcc gcgcgcggtc tccagcacct
      181 tggcgatctg cccgatcgtc cccgccgcgc ccgcgatgtc gaaccccgcc tcgtcgacgt
      241 atccgccctt cgaggcataa gcgttctgca tgtcgatgac gacaatcgcg gtctcctcga
      301 ccgcgatgcg gatcggctcc ggccgcgccg gcagggtgac gaagcgcttc gctgtctctc
      361 tcgccgcctc gaccgtcatc ccgtcactct ccaccagatg cgggaagggc acaacccatc
      421 cgtcaccccg gcgaaagccg gggcctcggg gagacatgca gcgtgtttct ggcccgaggt
      481 cccggcttcc gccgggacga cgatcatttc ggaaatcccc cctggaacgc gaactcctcc
      541 acccgtttcc gtccgctcgg cacctcccgc gcccgcaatt tctccggcaa ggtcgccgga
      601 tcgccgatcc gcccgatcgc caccgccgcc tcgatccgga aacgctcggg cacgccgagc
      661 tcgtcgcgcg cacgctcaaa ctgcaccccc gacatgccgt gcgcaaacca gcccatccga
      721 gtcgcctgca gcgccatcga cgcccacgcc gccccggcgt cgaagctgtg gctgtgcgag
      781 gccgccactc cacccgattt ggtctccacc aggctgtccg aaaggatgaa gaccagcacc
      841 gaagccgact gcgcccacgc ctgattccac ggaatcagca gccccagaaa cctttcccag
      901 tccgccccgc cgcgatgcgc ataaagaaac cgccacggct gcccattgaa cgccgaaggc
      961 gcccaccgcg ccgcctcgaa catcgccagc agctcctcct gggccatcgc cgccccatcg
     1021 aacgcgcgcg gcgaccagcg ttcgaggaag agcggatcga tcggatggtc ggcggtgcgg
     1081 gacatgggcg gctcctggct ggaccggcct gtgccactcc gccaccgccg gggacagtca
     1141 cttttcggca cggggcggcc cgccctcgtt cggagcacgc aaaagcagct atccccctac
     1201 cgccacgccg ctgcggcgga tcggtcacac ccgccccggt ttcgcctttg ttggccggca
     1261 agcgccttct tccggccgcg ccggcgggct attgcggggg cggaaggttg ttgttcatgc
     1321 tttgcttttt cggtcttcac agccgctcga tgagcgcgat gacggtgcgc cgcggtgcca
     1381 ccgtcagcct gtgcgagcgc tgcgcccgcc ccctcgtccg ccgccccgac ggccgctgga
     1441 cggcggcaga gccggtctga gccgcgccga cagggacagt cacttttccg ccgggcggcg
     1501 ccggtcctga tccggcgtgc gcaaaagtga ctgtccctt
//
LOCUS       DASVVN010000631         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_471503,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000631 DASVVN010000000
VERSION     DASVVN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471503"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VF686_10360"
     CDS             complement(<1..1039)
                     /locus_tag="VF686_10360"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX8662255.1"
                     /translation="MDPISERLRARHPQRIDLSLDRMRVLCAALGDPQHRLPPVVHVA
                     GTNGKGSTVAFVRAIAEAAGLKVHAYTSPHLVRFNERIRLGGQLIGDAALNDILDRIE
                     GLDVEATVFESTTAAAFVAMSETPADLAIVEVGLGGVLDATNVIERPLLSVITPVDLD
                     HAEFLGTSLPGVAAEKAGILKPGARGLIARQSEEAMAAIEAAALTVHAPLTVMGVDFD
                     AWAERGGMAFQDQERFLDLPAPALAGPHQIANAGLAVAVALELDLPEAAIAGGLRAVR
                     WPARLQRVTAGPHGEAARAADAELWLDGGHNPHAGRALAEALAERQAKAPRPLALIVG
                     MLANKDAGGFFE"
     gene            complement(1045..1938)
                     /gene="accD"
                     /locus_tag="VF686_10365"
     CDS             complement(1045..1938)
                     /gene="accD"
                     /locus_tag="VF686_10365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX8662256.1"
                     /translation="MVDKNKPPQEKRGGWLSRFAPGVRKIVSRRDTPENLWVKDPDTG
                     EMLYRSDLENSLWVTPSGRHMRINAEQRLRYTFDGGVFEAIDTPDVPEDPLKFSDGKP
                     YKDRLAAARKAAGRKDTMAIGFGKLDGAPAVVIVQDFSFMGGSLGMAAGEAFIAAARA
                     AIARGAPLVCYTAAGGARMQEGALSLMQMARSTLAIQELKAAQLPFVVVLTDPTTGGV
                     TASYAMLGDVHLAEPGALIGFAGPRVIEATIREKLPPGFQRAEYLQEKGMVDRVVARE
                     DLPKVLGQILSMLMGGRRRAA"
BASE COUNT          292 a          759 c          709 g          306 t
ORIGIN      
        1 cctcgaagaa gccgcccgcg tccttgttgg ccagcatgcc gacgatcagg gccagcggtc
       61 gcggcgcctt cgcctgccgc tcggccaggg cttccgccag cgcgcgaccc gcgtgcggat
      121 tgtgcccgcc atccagccac agctcggcgt cggccgcccg cgcggcctct ccatgggggc
      181 cggccgtcac ccgctgcagg cgcgccggcc agcgcacggc gcgcagaccc ccggcgatcg
      241 ccgcctcggg caggtccagt tccagcgcca cggccacggc gaggccggcg ttggcgatct
      301 ggtgcggccc ggccagcgcc ggggccggca gatcaaggaa tcgctcctga tcctgaaagg
      361 ccatgccgcc ccgctcggcc caggcgtcga aatccacgcc catgacggtg aggggcgcat
      421 gaacggtgag cgcggccgcc tcgatggccg ccatcgcctc ttccgactgg cgtgcgatca
      481 ggccgcgcgc gccgggcttg aggatgccgg ccttctccgc cgccaccccc ggcagtgagg
      541 tcccgaggaa ttccgcatgg tcgagatcga cgggcgtgat gacgctcagc agcgggcgct
      601 cgatcacgtt ggtggcgtcc agaaccccgc ccagaccgac ctcgacgatg gccagatcgg
      661 cgggcgtttc gctcatggcc acaaaggcgg cggcggtcgt gctttcgaac acggtcgcct
      721 cgacatccag cccctcgatg cggtcgagga tgtcgttcag ggcggcgtcg ccgatcagct
      781 gtccgcccag acggatgcgt tcgttgaacc ggaccagatg cggcgatgtg taggcgtgga
      841 ccttcaggcc cgcagcctcg gcgatcgccc ggacgaaggc gacggtcgag cccttgccgt
      901 tggtgcccgc cacatggacc acgggcggca gccggtgctg cggatcgccc agcgccgcac
      961 aaagcacccg catccggtcc agcgacaggt cgatgcgctg gggatgccgc gccctcaggc
     1021 gttcggagat cgggtccacg gcgctcaggc cgcccgacgc cgcccgccca tcagcatcga
     1081 caggatctgg cccagcacct tgggcaggtc ctcgcgcgcg accacgcggt cgaccatgcc
     1141 cttttcctgc agatattcgg cgcgctggaa gcccggcggc agtttctcgc ggatggtcgc
     1201 ctcaatgacg cgcggaccgg cgaagccgat cagggcgccc ggctcggcca gatgcacgtc
     1261 gcccagcatg gcgtagctgg cggtcacgcc gccggtggtc ggatcggtca gcaccacgac
     1321 aaagggcagc tgcgccgcct tcagctcctg gatggccagg gtcgaacgcg ccatctgcat
     1381 caggctcagc gcgccctcct gcatccgcgc cccgccggcg gcggtgtagc agaccagcgg
     1441 cgcgccgcgc gcgatggccg cacgggccgc ggcgatgaag gcctcgcccg ccgccatgcc
     1501 cagcgagccg cccatgaagc tgaagtcctg gacgatcacc accgcgggcg cgccgtccag
     1561 cttcccgaag ccgatggcca tggtgtcctt gcggccagcc gccttgcggg cggcggccag
     1621 acggtccttg tagggcttgc cgtccgagaa tttcagcgga tcctcgggca cgtcgggcgt
     1681 gtcgatggcc tcgaacacgc cgccgtcgaa ggtgtagcgc agccgctgct cggcgttgat
     1741 gcgcatgtgg cggcccgagg gcgtcaccca cagcgagttt tccagatccg accggtacag
     1801 catctcgccg gtgtcgggat ccttgaccca caggttctcg ggcgtgtcgc gccggctgac
     1861 gatcttgcga acgccgggcg cgaaccggct cagccagccg ccgcgttttt cctgtggggg
     1921 tttgttcttg tcgaccatgg cggcgcttta gacgctttct ttcacgcgcg caccccggac
     1981 cgcatcggcg aggacccgca ccttggccag aacgcgcgcc gcggcgggtt cgttcgcctc
     2041 aagcgcggcc gcgacctcgt cgacca
//
LOCUS       DASVVN010000632         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_525290,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000632 DASVVN010000000
VERSION     DASVVN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525290"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /gene="ispD"
                     /locus_tag="VF686_10370"
     CDS             complement(<1..678)
                     /gene="ispD"
                     /locus_tag="VF686_10370"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX8662257.1"
                     /translation="MTTPSPAFAAIVVAAGSGSRAGGAKQWRPLGGRPVVRWSVEALL
                     KAGADDVVVVVSAGSEAEAQAALAGLSGWRLATGGDTRAASVRNGLQTLGGPGGRPVL
                     IHDAARPFLEIAVIRRLIEALDDADGALPALPVADSLRRATDGLVSGAVERDGLWRAQ
                     TPQAFRYQAIVDAYAAWPEAEPATDEATVLERTGGRVRIVEGDARLMKLTFPEDFAMA
                     EALIPRQI"
     gene            743..1798
                     /gene="dusB"
                     /locus_tag="VF686_10375"
     CDS             743..1798
                     /gene="dusB"
                     /locus_tag="VF686_10375"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX8662258.1"
                     /translation="MAAAGIQIGDVWVPGRVLTAPMTGITDLPFRRLASRLGASYVAT
                     EMVAAAELARARPDVVRRAAVGEGLPLTVIQLVGRDPAAMAEGARMAEKAGADIVDLN
                     FGCPAKEVTGSACGSALMRTPELACRLMQAAVEATTRPVTVKMRLGWDDAHRNAPLLA
                     KRAEEIGIKAVTVHGRTRQQFYTGVADWTAVGEVKVAVGIPVIVNGDILTADDAREAL
                     RQSGADALMLGRGVYGRPWLAAHLERALADGTVLLEPEREERLAIVIEHLRASVAFYG
                     LPLGLKMFRKHLGWYIEQAPWPEDPLDRRAAKSRLCRLESPAEVERELTAVWRPLTHS
                     GNHAVDISPQVVEAGVA"
BASE COUNT          279 a          635 c          735 g          279 t
ORIGIN      
        1 gatctggcgg gggatgagcg cttcggccat ggcgaaatcc tcggggaagg tcagtttcat
       61 cagccgcgcg tcgccctcga cgatgcggac gcggccaccc gtccgttcca gcacggtggc
      121 ttcgtcggtg gcgggttcgg cttcgggcca ggcggcgtat gcgtcgacga tggcctggta
      181 gcggaacgcc tgcggggtct gggcgcgcca caggccgtcg cgctcgaccg cgcccgagac
      241 gaggccgtcg gttgcgcggc gcaggctgtc ggcgacgggc agggcgggca gggcgccgtc
      301 cgcgtcgtcc agcgcctcga tcagacggcg gatgacggcg atttcgagga agggtcgcgc
      361 cgcgtcatgg atcagaacgg gccggccgcc ggggccgccg agcgtttgca ggccgttgcg
      421 gaccgaggcg gcgcgggtgt cgccgcccgt cgccagccgc caacccgaaa gaccggcgag
      481 ggcggcctgc gcctcggctt ccgaaccggc ggaaacgacc acgacgacat cgtccgcccc
      541 ggccttcagc agggcctcga cggaccagcg cacgacgggc ctgccgccga gggggcgcca
      601 ttgcttggcg cctccggcgc gggagccgga accggcggcg accacgatgg cggcgaaggc
      661 gggggagggc gtggtcatcc ccggccttct aatcgccgcg gttgcgcttg acgagaggcg
      721 tcgacggcgc ggaaagggcc tcatggctgc tgcagggata cagatcggcg acgtgtgggt
      781 gcccggacgg gtgctgaccg cgcccatgac cgggatcacc gacctgccgt tccggcggtt
      841 ggcctcgcgg ctgggcgcat cctacgtcgc gaccgagatg gtggctgccg ccgaactggc
      901 ccgggcccgt cccgatgtgg ttcgacgcgc cgcggtgggc gagggtctgc cgctgaccgt
      961 gatccagctg gtggggcgcg atccggcggc catggccgag ggcgcgcgca tggcggaaaa
     1021 ggccggcgcc gacatcgtcg atctgaattt cggctgtccg gccaaggagg tcacgggctc
     1081 ggcctgcggc tcggccctga tgcggacgcc cgaattggcc tgccggctga tgcaggcggc
     1141 ggtggaagcg accacgcggc ccgtgacggt caagatgcgg ctgggctggg acgacgctca
     1201 ccgcaatgca cccctgctgg cgaagcgggc cgaggagatc gggatcaagg cggtcaccgt
     1261 gcacggccgg acccggcagc agttctacac gggcgtcgcg gactggaccg cggtcggcga
     1321 ggtcaaggtg gcggtgggca ttcccgtcat cgtcaacggc gacatcctga ccgccgacga
     1381 cgcgcgcgag gcgctgcgcc agtccggcgc cgacgccctg atgctgggcc gcggcgtcta
     1441 cggccgtcca tggctggccg cgcacttgga gcgggcgctg gccgacggga cggtgctgct
     1501 ggagcccgaa cgggaagaac ggctggctat cgtcattgag cacctgcggg cctcggtggc
     1561 cttctatggt ctgccgctcg ggctgaagat gttccgcaag catctgggct ggtacatcga
     1621 acaggcgccc tggcctgagg atccgctgga tcggcgcgcg gcgaaatccc ggctgtgccg
     1681 cctcgaaagt ccggcagagg tcgagcgcga gctcacggcc gtatggcgac cactgacgca
     1741 ttcgggcaac cacgctgttg atatttcgcc acaggtggtc gaagctggag tggcatgacg
     1801 gatacaccca ccgccgacgc agaacctgaa cccgccgctg aacgacgcga cgcggttcgc
     1861 tggttcgccg gttggtcgga ggaacggcgg cgcaacgtct tcctgttcgc ctacaccctc
     1921 gcatttct
//
LOCUS       DASVVN010000633         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_156591,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000633 DASVVN010000000
VERSION     DASVVN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156591"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VF686_10380"
     CDS             <1..423
                     /locus_tag="VF686_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8662259.1"
                     /translation="DQPGYHAQPMTAFREEETGTIWFFTRDDTDLAKDAAVGSGQSAM
                     FHYGSKDQNIWACIHGQLSVHGQDREIIDRYWNPILAAWYPEGKDDPHLTILQFDADD
                     GRIWVNEGGFFKFFYEVAKANVTKTLPDSGGVVDVNLN"
     gene            complement(420..1340)
                     /gene="argF"
                     /locus_tag="VF686_10385"
     CDS             complement(420..1340)
                     /gene="argF"
                     /locus_tag="VF686_10385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX8662260.1"
                     /translation="MTMVRHFLDIHRLEAEELRAILDDAHARKAARKSWPKGRVDADA
                     PARDRVLAMVFEKNSTRTRFSFDAAIRQLGGASIIANAGDMQLGRGEPVEDTARVLSR
                     MVDAVMIRANSHEDVERFARVATVPVINGLTDRSHPCQILADLMTIEEHRGPIAGKTI
                     AWVGDGNNVCHSFMHAAPKLGFHFNVACPADYHPDLHDLAKGGNHVTLTSDPREAVAG
                     ADVVVTDTWVSMGDGDYEARLEAFEGYGVDDRLMAGASSDAVFLHCLPAHRGEEVTDA
                     VIDGPKSLVWDEAENRIHAQKAVLAWCFAG"
     gene            complement(1337..>1700)
                     /locus_tag="VF686_10390"
     CDS             complement(1337..>1700)
                     /locus_tag="VF686_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8662261.1"
                     /translation="NPLAMAVGQAAFGEISKAETLGHVNEIAGYLKQQLHGLAQRFPD
                     VIVDVRGKGLLLGVKLVPNNREFMGWARDEQHLLVAGGGDNCCRILPPLNLTLEEARE
                     AVERFERTCEFARSKMAA"
BASE COUNT          272 a          557 c          584 g          287 t
ORIGIN      
        1 gaccagcccg gctatcacgc ccagccgatg accgccttcc gcgaggagga gaccgggacg
       61 atctggttct tcacccgcga cgacaccgac ctggcgaagg acgccgcggt gggctcaggc
      121 cagagcgcca tgttccacta cggctcgaag gaccagaaca tctgggcctg catccatgga
      181 cagctttcgg tgcatggcca ggatcgcgag atcatcgacc gctactggaa cccgatcctc
      241 gccgcctggt atcccgaggg caaggacgac ccgcacctga ccatcctgca gttcgacgcc
      301 gacgacggcc gcatctgggt caacgaaggc ggcttcttca aattcttcta cgaggtcgcc
      361 aaggccaatg tgaccaagac gctgccggac tccggcgggg tcgttgacgt caacctgaac
      421 tagcccgcga aacaccacgc cagcacggcc ttctgggcgt ggatgcggtt ttcggcctcg
      481 tcccagacca gcgacttcgg tccgtcgatg acggcgtccg tcacctcctc gccgcggtgg
      541 gcgggaaggc agtgcaggaa gaccgcgtcg ctgcttgccc cggccatcag ccgatcgtcg
      601 acgccatagc cctcgaaggc ttccagacgg gcttcatagt cgccgtcgcc catcgacacc
      661 caggtgtcgg tgacgaccac gtcggcgccg gccacggcct cgcgcggatc gctggtcagg
      721 gtgacgtggt tgccgccctt ggccaggtcg tgaaggtcgg ggtggtagtc ggcgggacag
      781 gcgacgttga agtggaagcc cagcttgggc gccgcatgca tgaagctgtg gcagacgttg
      841 ttgccgtcgc cgacccaggc gatcgtcttg ccggcgatcg gaccgcgatg ctcctcgatc
      901 gtcatcaggt ccgccagaat ctggcacgga tgcgagcggt cggtcaggcc gttgatcacc
      961 ggcacggtgg cgacgcgggc gaaacgttcg acgtcctcgt ggctgttggc gcggatcatc
     1021 acggcgtcga ccatgcgcga caggacgcgg gcggtgtcct cgaccggctc gccgcggccc
     1081 agctgcatgt cgccggcgtt ggcgatgatc gaggccccgc ccagctggcg gatggcggcg
     1141 tcgaagctga agcgggtgcg ggtcgaattc ttctcgaaga ccatggcgag gacccggtcc
     1201 ctggccggag cgtcggcgtc gacgcggccc ttgggccagc tcttgcgcgc cgcctttcgg
     1261 gcatgggcgt cgtcgaggat ggcgcgcagc tcttcggctt cgagccggtg gatgtcgagg
     1321 aagtgccgga ccatggtcag gccgccattt tcgaacgggc gaattcgcag gtgcgctcga
     1381 agcgttcgac ggcctcgcgg gcctcttcga gggtgaggtt cagcggcggc aggatgcggc
     1441 agcagttgtc gccgccgccg gcgaccagca ggtgctgctc gtcgcgcgcc cagcccatga
     1501 attcgcggtt gttggggacc agtttgacgc ccagcagcag acccttgccg cgcacatcca
     1561 cgataacgtc ggggaagcgc tgcgccagac cgtgcagctg ctgtttcaga tagccggcga
     1621 tctcgttgac gtggcccagg gtctcggcct tcgatatctc gccgaaggcc gcctggccga
     1681 cagccatggc cagcgggttg
//
LOCUS       DASVVN010000634         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_419976,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000634 DASVVN010000000
VERSION     DASVVN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419976"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VF686_10395"
     CDS             complement(<1..775)
                     /locus_tag="VF686_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8662262.1"
                     /translation="MSLATVVEPTLDNRQLLTALRAFRRGDFSVRLPGGLTGMDGELA
                     EAFNDVVELNDRMSREFSRLGEAVGRQGKINNRARVPSATGAWADSVDAVNTLIADMV
                     HPTAEMARVIGAVAKGDLSQTMDLENEEQPLRGEFLRIGRVVNTMVGQLGSFASEVTR
                     VAREVGTEGKLGGQANVKGVAGTWKDLTDNVNFMAANLTGQVRNIAEVTTAVANGDLS
                     RKITVDVKGEVLELKNTINTMVDQLNSFASEVTRVAREVG"
     gene            1026..1418
                     /locus_tag="VF686_10400"
     CDS             1026..1418
                     /locus_tag="VF686_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662263.1"
                     /translation="MSAAGQGGVEPELAAFIEAAVPSVWALEALIVLKRDPKIAWATD
                     DLVTEMRASTTLVADCVRVLQRAGLVIEQSGSVQYAPASPALAALADALQDAYRQRPV
                     AIVKMITARRTDPLKGFADSFRLGGWKG"
     gene            1415..1687
                     /locus_tag="VF686_10405"
     CDS             1415..1687
                     /locus_tag="VF686_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX8662264.1"
                     /translation="MTDWMPQLVYGLCVATSAICAVLLVRSWRRSRQPLLLWSACCFS
                     LLALNNLLLFLDRLVLREVDLGPARAATALLAVGVLLYGFIWEVDR"
     gene            1684..1965
                     /locus_tag="VF686_10410"
     CDS             1684..1965
                     /locus_tag="VF686_10410"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX8662265.1"
                     /translation="MSAMALANGAVIMGHVIAALFFVKFWRRTGDRLFLAFAAAFLLM
                     GLVPLLTNLMQVPREEQSPFYLIRLAAFLIIIWAIAAKSRSGPKQHPPE"
     gene            2019..>2540
                     /locus_tag="VF686_10415"
     CDS             2019..>2540
                     /locus_tag="VF686_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX8662266.1"
                     /translation="MSESRANPPEDRRPGFYEFFAGGGMVRAGLGEGWRCLFANDIDP
                     GKGRAYRANWGECDFHLGDIGAVTAGDLPGAAQLMWGSFPCQDLSLAGTGAGLEGRRS
                     GTFHAFWDVARGLAADGRAPAIVAVENVCGTLTSRGGRDFEVVCQTYVDAGYRWGALI
                     INADRFLPQSRPRL"
BASE COUNT          361 a          835 c          868 g          476 t
ORIGIN      
        1 tgccgacttc acgggccacg cgggtcactt ccgaggcgaa ggagttcagc tggtccacca
       61 tggtgttgat ggtgttcttc agctccagca cctcgccctt cacgtccacg gtgatcttgc
      121 ggctgaggtc gccgttggcc acggcggtgg tcacctcggc gatgttccgc acctgacccg
      181 tcaggttggc ggccatgaag ttcacgttgt cggtcaggtc tttccaggtc ccggccacgc
      241 ctttcacgtt cgcctgaccg cccagcttgc cctcggtgcc cacctcgcgg gccacgcggg
      301 tcacttcgga ggcgaacgag cccagctggc ccaccatggt gttcacgacc cggccgatgc
      361 gcaggaactc gccgcgcaga ggctgctcct cgttctccag atccatggtc tggctgaggt
      421 cgcccttggc cacggcgccg atcacgcggg ccatttccgc ggtcgggtgg accatgtcgg
      481 cgatcagtgt gttgacggcg tcgacgctgt cggcccaggc gccggtagcc gacggaacgc
      541 gggcgcggtt gttgatcttg ccctgccggc ccacggcctc gccgaggcgg ctgaactcgc
      601 ggctcatccg gtcgttcagt tcgacgacgt cgttgaaggc ctcggccagt tcgccgtcca
      661 tgccggtcag accgccgggg aggcggaccg agaaatctcc tcgccggaac gccctcaggg
      721 ccgtgagaag ctggcggttg tcgagggttg gctcaacgac ggtggcaagc gacatcattc
      781 accttcagct cagaagcagg cgcatccgaa ggttccgtcc gtcgtttgtc ggggagtttt
      841 gacgctgcgc tttcgcgctc gcactgcccc ccggccacgc ccacacgctc ccccgaacca
      901 actgcagaaa accgcgaagg ttggtaaggg ttcccggggg tcacgaagat tttgacgttg
      961 ccaatcggga ttcagaagct agcttcccgc gccatgcaca cgaagccaga tcgggcgttc
     1021 ggggcgtgag cgcggcggga caaggcggcg tcgagcctga attggcggcc tttatcgagg
     1081 cggccgtccc ttcggtatgg gcgctggagg ccttgatcgt tctgaagcgt gaccccaaaa
     1141 tcgcctgggc gacagatgat ctggtcacgg aaatgcgggc cagcacgacg ctggtagccg
     1201 actgcgtacg ggttctgcag cgcgccgggc tggtcatcga acagtcgggt tcggtccagt
     1261 acgctccggc ctcccccgcg ctggcggccc tggccgacgc gttgcaggat gcctatcgcc
     1321 aacggccggt ggccatcgtc aagatgatca cggcgcgcag aacggatccg ctgaagggat
     1381 tcgccgactc gtttcggctg gggggatgga agggatgacc gattggatgc ctcagctggt
     1441 gtacggtctt tgcgtcgcga ccagcgcgat ctgcgccgtg ctgctggtgc gatcctggcg
     1501 acggtcgcgc cagcccctgc tcctgtggag cgcgtgctgc ttcagcctgc tggcgctcaa
     1561 taacctgctt ctgttcctgg accggctggt tctgcgggaa gtcgatctag ggccggcgcg
     1621 cgcggcgacc gccctcctcg ccgtgggggt ccttctttac ggcttcatct gggaggtcga
     1681 tcgatgagcg cgatggcgct ggcgaacggc gcggtgatca tgggccacgt catcgcggcc
     1741 ctcttcttcg tcaaattctg gcgtcggacc ggcgatcgcc tgttcctggc gttcgccgcc
     1801 gcctttctgc tgatgggcct ggtgccgctg ctgacgaacc tgatgcaggt gccgcgcgag
     1861 gagcagagcc ccttctacct gatccgcctg gccgccttcc tgatcatcat ctgggccatc
     1921 gcggccaagt cccgatcggg gccgaaacag cacccgcccg agtagatccc ggagcgcttt
     1981 cattggactt ggcggcgccg gagcgtcagc gtcgggacat gagcgaatcg cgcgccaatc
     2041 cgcccgagga ccggcggccc ggcttctacg aattcttcgc cggcggcggc atggtccggg
     2101 ccgggctggg cgagggctgg cgctgcctgt tcgccaacga catcgatccg ggcaagggcc
     2161 gggcctatcg cgccaactgg ggcgagtgcg actttcatct gggcgacatc ggcgcagtga
     2221 cggcaggcga cctgcccggc gcggcccagc tgatgtgggg ctccttcccc tgtcaggacc
     2281 tttcgctggc gggaaccgga gccgggctgg aagggcgccg gtccgggacg tttcacgcct
     2341 tctgggacgt ggctcgcgga ctggcagccg acggacgggc gccggcgatc gttgcggtcg
     2401 agaatgtgtg cggcacactg acctcgcgcg gcgggcggga tttcgaggtc gtctgccaga
     2461 cctatgtcga cgccggctat cgctggggcg ccctgatcat caacgccgac cggttcctgc
     2521 cccagtcgcg gccgcgcctg
//
LOCUS       DASVVN010000635         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_156633,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000635 DASVVN010000000
VERSION     DASVVN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156633"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VF686_10420"
     CDS             <1..1384
                     /locus_tag="VF686_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX8662267.1"
                     /translation="AVAAADFPAAILRVRNDRAAASVGLDTLTDAEWISHFGRFEPLP
                     GQPGPVAMRYHGHQFRTYNPDLGDGRGFLAAQLRERPQAASARGASARENKAGRLLDL
                     GTKGSGQTPWSRGGDGRLTLKGGMREVLAAEMLEALGVPTSRAFSLIETGEALQRGDE
                     PSPTRSAVLVRLSHSHIRFGTFQRAAYLGRKDLIEALVEHARTLYHPAVAPGDTPGFL
                     RAVVEASARLTARWIAAGFVHGVLNTDNLNVTGESFDYGPWRFLPHYEPGFTAAYFDQ
                     TGLYAFARQPEAVFWALTQLGGALKLAAEAEPLTEALNGFGPAYIRELRAAFLARLGV
                     QSLGEAADQRLLDATLALLREGGEALRWEPLFFDWFGGFASSARALGGPRGKLYQGET
                     FDAFRFALFEHAPDRPERLEHPVFGRLEPEEMLIDEVEAIWTAIDDDDDWSPLYDKLA
                     RLRAVRTSLA"
     gene            1484..>1713
                     /locus_tag="VF686_10425"
     CDS             1484..>1713
                     /locus_tag="VF686_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662268.1"
                     /translation="MHTETSPTRSNWSPSQTEAILLVVMVAIVSDMPEPVPFWPDAFG
                     QAPSVGTVLLIACVILAIARFGRRSDTRTDIAR"
BASE COUNT          257 a          586 c          587 g          283 t
ORIGIN      
        1 cgccgtcgcg gcggcggact ttccggcggc gatcctgcgc gtccgcaacg accgggcggc
       61 cgcaagcgtc ggtctggaca ccctgaccga cgccgaatgg atttcccatt tcggccggtt
      121 cgagcccctg cccggccagc ctggccccgt ggccatgcgc tatcacggtc accagttccg
      181 cacctacaac cccgacctcg gcgacggacg cggctttctg gcggcccagc tgagggaacg
      241 ccctcaagca gcgagcgccc gtggcgcgag cgccagggaa aacaaagccg gccggttgct
      301 cgatctgggc accaaggggt ccggtcagac gccgtggtcg cgcgggggag acgggcggct
      361 gacgctgaag ggcgggatgc gcgaggtgtt ggcggccgaa atgctggagg cgctgggcgt
      421 gcccaccagc cgggccttca gcctgatcga gaccggcgag gccctgcagc gcggcgacga
      481 accctcgccg acgcggtcgg cggtgctggt gcggctgtcg cacagccaca tccgtttcgg
      541 caccttccag cgggcggcct atctgggccg caaggacctg atcgaggcgc tggtcgagca
      601 tgcgcgcacc ctctaccacc cggcggtcgc ccccggcgat acgcccggtt tcctgcgcgc
      661 cgtggtcgag gcctcggcgc ggctgacggc ccggtggatc gcggcgggct tcgtccacgg
      721 ggtgctgaac accgacaatc tgaacgtcac cggcgaaagt ttcgactacg gcccgtggcg
      781 gttcctgccg cattacgagc ccggcttcac cgccgcttat ttcgaccaaa ccggcctgta
      841 cgccttcgcg cgtcaacccg aggcggtgtt ctgggcgctg acccagcttg gcggggcgct
      901 gaagctggcc gccgaggccg agcccctgac cgaggctctg aacggtttcg gcccggccta
      961 tatccgtgaa ttgcgcgccg ccttcctggc gcggctgggt gtgcagagcc tcggcgaggc
     1021 ggccgaccag cggctgctgg atgcgaccct ggccctgctg cgcgaaggcg gcgaggccct
     1081 gcgctgggag ccgctgttct tcgactggtt cggcggtttc gcctcgtccg cccgggcgct
     1141 cggcgggccg cgcggcaaac tttatcaagg cgagaccttc gacgccttcc gcttcgccct
     1201 gttcgaacac gcccccgacc gcccggaacg gctggagcat ccggtcttcg gacggctcga
     1261 gcccgaggaa atgctgatcg acgaggtcga ggcgatctgg accgccatcg atgacgacga
     1321 cgactggtcg cccctctatg acaagctggc gcggttgagg gctgtccgga caagcctggc
     1381 ctgacgcgaa aggaaagctt ccttggcatg atgtaaaatg tgcttgactg gatcaggccg
     1441 ggatatgtaa agcagtcttg tcataacgac agggaggctt gtcatgcaca ccgaaaccag
     1501 cccgacccga tcaaactggt cgccgtcgca gaccgaggcg attctccttg tggtgatggt
     1561 cgccatcgtc tcggacatgc ccgaaccggt ccctttctgg ccggacgcgt tcggacaggc
     1621 gccctcggtc ggcacggtgt tgctgatcgc atgcgtcatt ctggcgatcg cccggttcgg
     1681 gcgccgttcc gacacccgga cggacatcgc gcg
//
LOCUS       DASVVN010000636         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_314619,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000636 DASVVN010000000
VERSION     DASVVN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314619"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..680)
                     /locus_tag="VF686_10430"
     CDS             complement(63..680)
                     /locus_tag="VF686_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662269.1"
                     /translation="MLRRKELPLLDPQSWDDRNDRYFMGLYKEKRQLGGLYGLCAAQC
                     SETYHHWRVFTGTSDGACIELKRDPLEAALGKIDGVRFGPVEYLRLPDVEKLTAGDVD
                     RLPFVKRIGFEAEDEYRIVAETSAAQAPVMGIGMPLSWVGAVHLNPWLPRPIADSVKA
                     TLKEIPGCSKLRVVGSQLIDSGRWKQAGDTVVGKPRPKPRLVLKR"
     gene            complement(768..1520)
                     /locus_tag="VF686_10435"
     CDS             complement(768..1520)
                     /locus_tag="VF686_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8662270.1"
                     /translation="MTDLPLKDRIALVVGASRGIGYETALALAKAGAHVIATARTTGG
                     LEELDDAIFAATGKHATLIPFDLVDGGAIDRLGGAIYERFGRLDVWVQAAATMGASGL
                     TPVSHADPRVWAKVEKTNFTAVYRLIRSLEPLLKASDAGRAIHVTSSVASAPKAFWGA
                     YAASKAGAEALVKCWADEIESTPVRVAIVDPGRMRTAMRAQAYPGEDPEDVIHPSAIG
                     PLMVELARGDLEPALEVKFKDWSGGPNVAALI"
     gene            complement(1557..1901)
                     /locus_tag="VF686_10440"
     CDS             complement(1557..1901)
                     /locus_tag="VF686_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8662271.1"
                     /translation="MIQLGYFTIDTQDVDKAKAFYSALFGWTFDEDASKPTYAHVSGS
                     NPAFGFTKVERAGQFPHLYFRVDDVDAVCKRVAELGGKAAVPADSASGRTAVVSDDQG
                     VSFSLWQPAPGF"
     gene            complement(1932..3446)
                     /gene="purF"
                     /locus_tag="VF686_10445"
     CDS             complement(1932..3446)
                     /gene="purF"
                     /locus_tag="VF686_10445"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX8662272.1"
                     /translation="MSRLEHSIAAPVVHREHYRDADDDRPRLECGVCGVWGAEKDAAS
                     SVVALGLHALQHRGQEACGMASVNDTRFHTERHMGLVGEAFGGADLSTRMPGSAAVGH
                     TRYSTAGGSFLRNIQPMFADLDQGGIAIAHNGNLTNFLYLRNQLVGEGAIFQSTSDSE
                     VILHLIARSRKAKIVDRFIDAIGRIEGGYALVCQTRSKMIGARDPLGIRPLVLGRLDD
                     AWVLASETCALDTIGATFERDVEHGEVVVIDHEGLKSLKPFPTRGARPCLFEYVYFSR
                     PDSVVNGRSVYGVRKRMGMRLAREHAVEADVVVPVPDSGVPAALGYAQESGIPYEMGI
                     IRSHYLGRTFIQPSQGARQKGVRMKHSPNRSVLEGKRVVLIDDSIVRGTTSVKLVRAV
                     RAAGATEVHLRSASPPILWPDFYGIDMPERDQLIAANKTLEEMRAHLEVDSLGFLSVD
                     GLYDAMEAGPRDPRNPQFTDHYFTGEYPTRLLDREIEEGGREVAARQLSLLVSA"
     gene            complement(3474..>3858)
                     /locus_tag="VF686_10450"
     CDS             complement(3474..>3858)
                     /locus_tag="VF686_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX8662273.1"
                     /translation="LLIYFGIRIFGSILSKRAQAHPQLGSIDRIVGIFVGAARSLVLL
                     GAIHLVIVAAMPGDKTPRWLSEAALRPVTAGAARMIQIILPGIGRGADALTPVVTSSV
                     SKGFSEEKALPSPQTSNTSPPSAAR"
BASE COUNT          618 a         1309 c         1294 g          637 t
ORIGIN      
        1 gtgtcgcgtt caggccgcgt gcggaccggg cagaagcacg cgccgaaaaa gtaggccggc
       61 gctcagcgtt tgagcacaag ccggggtttc ggcctcggct tgccgaccac agtgtcgccc
      121 gcctgcttcc agcgtccgct gtcgatcagc tgcgagccca cgacccgcag tttgctgcac
      181 cccgggatct ctttcagcgt ggccttgacg ctgtctgcga tcggccgtgg cagccatggg
      241 ttcagatgaa cggccccgac ccagctcagg ggcatcccga tacccatgac cggggcctgt
      301 gcggccgagg tttcggccac gatccggtat tcgtcctcgg cctcgaagcc gatgcgcttc
      361 acgaacggca ggcgatcgac gtcaccggcg gtcagcttct cgacgtccgg aagcctgaga
      421 tattcgaccg ggccgaaccg cacgccatcg atcttgccga gcgccgcctc gagcgggtcg
      481 cgtttcagtt cgatgcaggc gccgtcggac gtgccggtga agacgcgcca gtggtggtag
      541 gtttccgagc actgggccgc gcacaggcca taaagcccgc cgagttggcg cttctccttg
      601 tacagaccca tgaagtagcg gtcgttccgg tcatcccagc tctgcggatc cagcagaggc
      661 agctccttgc gccgcagcat gtttatgacg ctcgagatcg acgtgtatct gcgaatgtat
      721 cccgtaagtc gcggcattcc gggctcctgc tgggtctccg gcgacgatca gatcagggcc
      781 gccacgttcg gtccaccact ccagtccttg aacttcacct ccagcgccgg ctccagatcg
      841 ccgcgcgcca gttcgaccat cagcgggccg atggccgagg ggtgaatcac gtcctccgga
      901 tcctcgcccg gataggcctg ggctcgcatc gccgtgcgca tccggcccgg atcgacgatg
      961 gcgacccgca ccggcgtcga ctcgatctcg tcggcccagc atttgaccag cgcctcggcc
     1021 cccgccttgg acgccgcata ggcgccccag aaggccttgg gcgcgctggc gacgctgctg
     1081 gtcacatgga tggcccgtcc ggcgtccgac gctttcaaca agggttcgag cgagcggatc
     1141 agccggtaga cggcggtgaa attggtcttc tctaccttgg cccagacgcg cgggtcggca
     1201 tggctgaccg gcgtcaggcc cgaggctccc atcgtggccg ccgcctgcac ccagacgtcc
     1261 agccgcccga accgctcgta gatcgcgccg cccagccggt cgatggctcc gccgtccacc
     1321 agatcgaacg ggatcagggt cgcgtgcttg cccgtggcgg cgaaaatggc gtcgtccagc
     1381 tcttccagcc cgcccgtcgt gcgcgccgtt gcgatgacat gcgcgcccgc cttggccaga
     1441 gccagggcgg tctcataccc gatgccgcgc gaggccccga cgacgagggc gatgcggtct
     1501 ttgaggggaa gatcggtcat gcggggctga tagcccgccc gccggtccgg cgaaagtcag
     1561 aagccgggcg ccggttgcca caggctgaag ctgacgccct gatcgtcgct gaccacggcc
     1621 gtccgtccgc tcgcgctgtc cgccggcacc gccgccttgc cgcccagctc agccacgcgc
     1681 ttgcacaccg cgtccacgtc atcgacgcgg aaatacaggt gcggaaactg gccggcccgc
     1741 tcgaccttgg tgaagccgaa ggcggggttg ctgcccgaga cgtgcgcata ggtcggcttg
     1801 gaggcgtcct cgtcgaacgt ccagccgaac agggccgagt agaaggcctt ggccttgtcg
     1861 acatcctggg tgtcgatggt gaagtagccg agctggatca tccgccgcct ctgaagggga
     1921 tcgtatgaac cttaagcgct gaccagcagt gaaagttgcc gcgccgccac ctcgcgtccg
     1981 ccttcctcga tctcgcgatc cagcagccgg gtcgggtatt cgccggtgaa atagtggtcg
     2041 gtaaactgcg gattgcgcgg atcgcggggc cccgcctcca tggcgtcgta caggccgtcg
     2101 accgacagga agcccagcga gtcgacctcc agatgggccc gcatctcttc cagcgtcttg
     2161 ttggcggcga tcagctggtc ccgctcgggc atgtcgatgc cgtagaagtc gggccacagg
     2221 atcggcggcg aggccgaacg caggtgcacc tcggtcgcgc ccgccgcgcg cacggcgcgg
     2281 accagtttca ccgatgtggt gccacgcacg atggaatcgt cgatcagcac gacccgcttg
     2341 ccttccagca ccgagcggtt ggggctgtgc ttcatgcgca cgcccttctg ccgggcgccc
     2401 tggctgggct ggatgaaggt gcggcccaga tagtggctgc ggatgatccc catctcatag
     2461 ggaatgccgc tctcctgcgc atagcccagc gccgcgggca cgccggagtc cggcaccggc
     2521 acgaccacat cggcctccac cgcatgctcg cgggccagcc gcatgcccat ccgcttgcgc
     2581 acgccataga ccgagcggcc gttcacgacg ctgtcggggc gcgagaaata gacgtattcg
     2641 aacaggcagg ggcgggcccc gcgagtgggg aagggcttga gcgacttgag accctcgtga
     2701 tcgatcacga ccacctcgcc atgctccacg tcgcgttcga aggtcgcgcc gatggtgtcc
     2761 agcgcgcagg tctcggacgc cagcacccag gcgtcgtcca gcctgcccag caccagcggg
     2821 cgaataccca gcggatcgcg ggcgccgatc atcttggacc gcgtctggca gaccagggcg
     2881 tagccgccct cgatccggcc gatggcgtcg atgaagcggt cgacgatctt ggccttccgg
     2941 ctgcgcgcga tcaggtgaag gatgacctcc gagtccgagg tcgactggaa gatggcgcct
     3001 tcgccgacca gctggttccg cagatagagg aagttggtca ggttgccgtt gtgggcgatg
     3061 gcgatgccac cttggtccag atcggcgaac atcggctgga tgttgcgcag gaagctgccg
     3121 ccggcggtgg aatagcgggt gtggccgacg gccgccgagc ccggcatccg cgtcgacagg
     3181 tccgcgccgc cgaaggcctc cccgaccaga cccatgtggc gttcggtgtg gaagcgggtg
     3241 tcgttgacgc tcgccatgcc gcaggcttcc tggccgcggt gctgcagggc gtgcaggccc
     3301 agggcgacga ccgacgacgc cgcgtccttt tccgcgcccc agacgccgca cacgccgcat
     3361 tccagacgcg gacgatcatc gtcggcgtcg cgatagtgct cccgatgaac gaccggggcg
     3421 gcgatggaat gctcgaggcg gctcatcgtg ggctccgatg aagcggggga acgctagcgt
     3481 gcggcggacg gcggcgaggt attgcttgtt tggggcgagg gcaaggcttt ctcttcggaa
     3541 aaccctttgc tcacggacga agtgaccacc ggtgtcagcg catcggcgcc ccggccgatg
     3601 cccggcagta tgatctggat cattcgcgcc gcgcccgccg tcacgggccg cagggccgcc
     3661 tcgctcaacc agcgcggcgt cttgtcgccg ggcatggcgg cgacgatgac cagatggatg
     3721 gcccccagca gcaccagtga tcgcgccgcg ccgacgaata tgcccacgat ccggtcgatg
     3781 cttccgagtt gcggatgcgc ctgcgcccgc ttcgacagga tggagccgaa gatgcggatg
     3841 ccgaaataga tcagcagg
//
LOCUS       DASVVN010000637         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_130553,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000637 DASVVN010000000
VERSION     DASVVN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130553"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..521
                     /locus_tag="VF686_10455"
     CDS             54..521
                     /locus_tag="VF686_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662274.1"
                     /translation="MTLPSTLNLARAALYAGLLAGALDIAAAILTNLQVPARAVLQSV
                     ASGLLGAPAYKGGWPTAWLGLAAHFAIMLVIALVFVAAATAMPALRRFWLPSGILFGV
                     AVWIVMNWIVVPMSASPISGPVTLMAALKPIAVHIVAVGLPIAWVTRRMLGAV"
     gene            complement(533..952)
                     /locus_tag="VF686_10460"
     CDS             complement(533..952)
                     /locus_tag="VF686_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8662275.1"
                     /translation="MAATPHANGFTVTGIDHIVLRVTDLDRSLDFYTRVLGCPVERRR
                     DDLGLIQLRAGSSLIDLVPVDGVIGQRGGAAPGPDAHNMDHMCIRIAAFDPEAAREHL
                     RRHGVEVESEGVRYGAGGNGMSFSITDPDGNGVELRA"
     gene            1039..1563
                     /locus_tag="VF686_10465"
     CDS             1039..1563
                     /locus_tag="VF686_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8662276.1"
                     /translation="MTAAPDHIDRLQAQWARELPDVDTRGMEVLGRARRIVLSSRPAI
                     EAVFARHGLDAGEFDVLSTLRRSGSPWALRPTELYRSLMISSGGLTDRLNRLVRSGMI
                     TRPPSPEDGRSLLVQLTDKGRAAAETAFREDMALEAGMLAVLSDAERRALADLLRKLA
                     LGMEASQAAQNADG"
     gene            1635..>2131
                     /locus_tag="VF686_10470"
     CDS             1635..>2131
                     /locus_tag="VF686_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX8662277.1"
                     /translation="MASLKQIRSTFLDYFAADGHEKVHSAPLVPQNDPTLLFVNAGMV
                     PFKDYFTGAAKPPYPRATSSQKCVRAGGKHNDLDNVGYTARHLTFFEMLGNFSFGDYF
                     KEHAIDRAWTLVTKDFGLDPNRLLVTVYIDDDEAAAIWKKVTGFGDDKIIRIAGSDNF
                     WAMGD"
BASE COUNT          326 a          769 c          668 g          368 t
ORIGIN      
        1 ttttcttgtg ataacggcca ggctgtccga ggctccggcc ggaggacccg acgatgaccc
       61 tgccgtccac gctcaacctt gcgcgcgccg ccctctacgc cggccttctg gcgggcgcgc
      121 tcgatatcgc cgcggccatc ctgaccaatc tgcaggtccc ggcccgcgcc gtgctccagt
      181 ccgtggccag cgggcttctg ggcgcccctg cttacaaggg cggctggccg acggcctggc
      241 tgggtctggc cgcccatttc gccatcatgc tggtcatcgc cctggtcttc gtcgccgccg
      301 ccacggcgat gccggcgctg cgccgcttct ggctcccctc cggcatcctc ttcggcgtcg
      361 cggtctggat cgtcatgaac tggatcgtcg tcccgatgtc ggcctcgccg atcagcggcc
      421 ccgtcaccct gatggccgcg ctgaagccga tcgccgtgca catcgtcgcc gtcggcctgc
      481 cgatcgcctg ggtgacccgg cggatgctgg gcgccgtctg accgcccggc cgtcaggccc
      541 gcagttcgac accgttaccg tcggggtcgg tgatcgagaa ggacatgccg ttcccgcccg
      601 ccccgtaccg gacgccctcg gactcgacct cgacgccgtg gcggcgcagg tgctcgcggg
      661 cggcctcggg atcgaaggcg gcgatgcgga tgcacatgtg gtccatgttg tgggcgtcgg
      721 gtcccggagc cgcgccgccc cgctggccga tgaccccgtc caccggcacc aggtcgatca
      781 gcgacgatcc cgcgcgcagc tggatcaggc ccaggtcgtc gcgccggcgc tccaccgggc
      841 accccagaac gcgggtgtag aagtccagcg atcggtccag atccgtgacc cgcagcacga
      901 tgtggtcgat gccggtgacc gtgaagccat tggcgtgagg agtggcggcc atgtcgtttc
      961 tccttggtgg gaatgccata tccagttatc ttgacgccaa gataatgtct tttgtctcga
     1021 cgtcaagata gtttctcaat gaccgctgcg cccgaccata tcgatcgtct tcaggcccaa
     1081 tgggcccggg agcttccaga cgtggacacc cgcggcatgg aggtgctggg cagggcccgc
     1141 cgcatcgtcc tgagcagccg cccagccatc gaggccgtct tcgcccgcca tggtctggac
     1201 gccggagagt tcgacgtcct ttcgaccctg cgccgctccg gctcgccctg ggcgctgagg
     1261 ccgaccgagc tgtaccgctc cctgatgatc tcgtcgggcg gcctgaccga ccggctgaac
     1321 cggctggttc gctccggcat gatcacccgc ccgccctcgc ccgaggatgg acgcagtctg
     1381 ctggtccaac tgaccgacaa gggtcgcgcc gccgccgaaa ccgcgtttcg cgaggacatg
     1441 gccctcgagg ccggcatgct ggcggtgctc agcgacgcgg aacgcagagc cttggccgac
     1501 ctcctgcgca agctggccct cggcatggaa gcgtcacagg cggcgcagaa cgccgatggg
     1561 tgatgcgctc ggggcgaaac acctatatgg acgctgcctc ccctgacctc attcagacgc
     1621 gtttgcgacc caccatggcc agcctcaaac agatccgctc gaccttcctc gactatttcg
     1681 cggccgacgg gcacgagaag gtccactcgg ctccgctggt gccgcagaac gacccgaccc
     1741 tgctgttcgt caacgcgggc atggtgccgt tcaaggacta tttcaccggc gcggccaagc
     1801 cgccctaccc gcgcgccacc agctcgcaga aatgcgtgcg cgccggcggc aagcacaacg
     1861 atctggacaa cgtcggctac accgcccggc acctgacctt cttcgagatg ctcggcaact
     1921 tctccttcgg cgactatttc aaggaacacg cgatcgatcg cgcctggacc ctggtcacca
     1981 aggatttcgg actcgacccc aaccgcctgc tggtcacggt ctacatcgat gacgacgagg
     2041 ccgccgcgat ctggaagaag gtcaccggct tcggcgacga caagatcatc cgcatcgccg
     2101 gctcggacaa tttctgggcc atgggcgaca a
//
LOCUS       DASVVN010000638         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_420252,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000638 DASVVN010000000
VERSION     DASVVN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420252"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VF686_10475"
     CDS             <1..1167
                     /locus_tag="VF686_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8662278.1"
                     /translation="WVAGLAPWSEGDVYDPEDVAELERRLLETGVFDGVGVALATADQ
                     GLANGNRPIVVTLTDRPRRILEAGATFSTAEGSGLDLLWTWHNRFGRADTLVWQARAA
                     NIDSRIGANLSLPHWRRPGETLKLSAALLNEDTDAYIRTAVALAADLQQRIGKTSYIS
                     YGVGLDAGKYDENRFDPVTQLPINTERNLAILTGRASAYLDRSNDPLNPTTGWRLSIW
                     AQPTAVSGDDSLAFLRAEAQVTAYLPLQDNAKTVLAGRARLGSIVGGDELAIPSDRLF
                     YSGGGGSVRGFEYQGVGPRLPDNTPRGGLSLVEAAIEVRHDVWRNFQAVAFIDAGAVG
                     FQEAPDFSNLRYGAGVGVRYMLPFGPIRADIAFPLDRRDGDADFQLYVSIGQAF"
     gene            1164..>1718
                     /locus_tag="VF686_10480"
     CDS             1164..>1718
                     /locus_tag="VF686_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662279.1"
                     /translation="MTAAPPPADAPDTPAETAKKKRTRLQAIAFFGGMGAAILAALLI
                     VALVGGRMYLLSGPGRELVTSFVAGKKLGRYGRINVEGVRGDLFDDFTIDRVTVTDAR
                     GVWLEARKVRVDWDWWPLIAREFHATNVEAQVIRLIRRPEVEPSTTPPGPQPLSVDID
                     RFSADVELLEGFSREYGRWRLTGEA"
BASE COUNT          265 a          629 c          554 g          270 t
ORIGIN      
        1 tgggtggccg gtctcgcccc atggtccgaa ggcgacgtct atgatcccga ggatgtcgcc
       61 gaactggagc gccggctgct ggagaccggc gtctttgacg gcgtgggcgt ggccctcgca
      121 acagccgatc agggcctggc caacggcaac cgccccatcg tcgtcaccct gaccgaccgg
      181 ccgcgccgta tcctcgaggc gggggcgacc ttctcgacgg cggaaggctc gggcctcgat
      241 ctgctctgga cctggcacaa ccggttcggg cgcgccgaca ccctggtctg gcaggcgcgc
      301 gcggccaata tcgacagccg catcggcgcc aacctcagcc tgccgcactg gcggcgtccg
      361 ggcgagacgc tgaaactctc cgccgccctc ctgaatgaag acacggacgc ctatatccgg
      421 acggccgtcg ccctcgccgc cgacctgcag caacgcatcg gaaagacgtc ctacatcagc
      481 tacggcgtcg gtctggacgc cggcaaatac gacgaaaatc gcttcgaccc ggtcacccag
      541 ctgccgataa acaccgagcg caatctggcg atcctgaccg gccgcgccag cgcctatctg
      601 gaccggtcga acgacccgct gaaccccacg accggctggc gtctctccat ctgggcccag
      661 cccaccgccg tgagcggcga cgattccctc gccttccttc gcgccgaggc ccaagtcacg
      721 gcctatctgc cgcttcagga caatgcgaaa accgttctgg cgggacgggc gcggcttggc
      781 tcgatcgtcg gcggcgatga actcgccatt ccgtccgacc ggctgttcta ttccggcggc
      841 ggaggttcgg tccgcggctt cgagtatcag ggcgtcggcc cccggctccc cgacaacacc
      901 ccgcgcggcg gccttagcct ggtcgaggcc gcgatcgagg tccgccacga tgtctggcgc
      961 aacttccagg ccgtggcctt catcgacgcc ggcgcggtcg gcttccagga agccccggac
     1021 ttcagcaacc tgcgctacgg cgcgggggtc ggcgtgcgtt acatgttgcc gttcgggccc
     1081 atccgggccg acatcgcctt cccgctggat cgccgggatg gcgacgccga cttccagctc
     1141 tacgtcagca tcggacaggc gttttgaccg ccgccccgcc gcctgcggac gcgcctgaca
     1201 cgccggcgga aacggcgaaa aagaagcgca cgcggctgca ggccatcgcc ttcttcggcg
     1261 gcatgggcgc cgctatcctc gccgcccttc tgatcgtcgc cctggtcggc ggccgtatgt
     1321 atctgctctc gggacccggc cgcgaactgg tcaccagctt cgtcgccggc aagaagctcg
     1381 gccgctatgg ccggatcaac gtcgagggcg tgcgcggcga cctgttcgac gacttcacca
     1441 tcgaccgcgt caccgtcacc gatgcgaggg gcgtctggct ggaagcgcgc aaggtccgcg
     1501 tcgactggga ctggtggccg ctgatagcgc gggagttcca cgcgacaaac gtggaggccc
     1561 aggtcatccg cctgatccgt cgtcccgagg tcgagccttc gaccacaccg cccgggccgc
     1621 agcccctgtc cgtggacatc gaccgcttct ccgccgacgt ggaactgctg gaaggcttca
     1681 gccgcgaata cggccgctgg cgtctgacgg gcgaggcc
//
LOCUS       DASVVN010000639         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_445406,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000639 DASVVN010000000
VERSION     DASVVN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445406"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..686
                     /locus_tag="VF686_10485"
     CDS             36..686
                     /locus_tag="VF686_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8662280.1"
                     /translation="MTYTVYGAAGSGSVPVEAALTLIGAPYRVVEAVTWEGEAERDKV
                     APVNPMRQIPALITPDGETITESAAILIWLAEQHPEAALGPEPCDPRRAQFLRWMTFI
                     PASVYSMFWVRDEPSRLGGDDPAAQKTIRERTAGRIADCWAKMESQILPDRFLLGPEP
                     TVLDLYVAVASRWTPNRKRFHEVAPRMGAVMRRVDALPELEAFWAERFPFEGRYGS"
     gene            complement(683..2848)
                     /locus_tag="VF686_10490"
     CDS             complement(683..2848)
                     /locus_tag="VF686_10490"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8662281.1"
                     /translation="MKGRWRLFRHEAGRVAGPPAWAYVLLFAASQLIGHWSVATYDAV
                     LIWLSNGIMAAALLQLHRRQAVTVVAACLVINLSSNAIRGDAGMLMWLNAVMNLGEAV
                     LAAVLARRFCGAALDMRRPRRLFAFGLFAALPAVAATAALGFGLTMLIRQTPLTVAGA
                     FGIYSFIIVELLGLLLVTPVLLLMARAHRFHGTTRARWPEATALLGLMLVVTTLAFWQ
                     NDAPLGFLCFLPMLLIALRLSPTTAAIALIGMTAVSGVATLTGFGPVHLTRLADIPGL
                     EAVPPMLRRLGVYNLFLLAMVVTVLPVSTVMTERRRLEARLRARTVAAQEARRKAEDA
                     AAARSRFLAMMSHEMRTPLNGVAGFADVLAGRPGLDATALRQARQIRESSDGLLMLVE
                     DILDFARGDDAVTPEALDLAAVVREAIAPSRAAADAKGLSLTIDDRLPSNARFMCDRR
                     ALRQTLHPMVANAVKFTDEGQVAIRLDRSSDGVCIRVSDTGSGIDPVLLPDLFEAFAQ
                     GDASIRRSHCGAGLGLALVARHVRRLNGHIDVDSRPGEGSTFIITLPLPRAPDAIEES
                     ERPMPDIRDEETGEAAAPATPRVLVVDDHPVNREVARIMLEAFGCDVVEVCDGQDAID
                     AVAGQPFDLVLMDVRMPLMDGLEATRRIRALPGPEHDIAVVAMTADAMPEDVIRCLAS
                     GMNAHMAKPINQAGLLAMVNRALSGDLPEARVSEAAEAA"
     gene            3012..3791
                     /locus_tag="VF686_10495"
     CDS             3012..3791
                     /locus_tag="VF686_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662282.1"
                     /translation="MSDTVAEPSFHPPEEAVAFYEESLRLLKESGIPFLLSGTYAVTA
                     YTGIQRPTKDLDVFCKPGDYPRILAFFQARGYRTDVEDERWIAKVWKDEKHFFDVIFA
                     MSNGTIAVNDAWFGPDTIEVYGHEVTITSPTALILSKVFIQDRYRYDGADVNHIILKQ
                     SGAIDWKSLLDQMDLYWEVLMSHLLNFRFAYPTERGNIPAWLMHELTERLRAQVDLPA
                     PRVKVCRGRLFSARDYISDISEWGFGDIVGKGLEERHDPIH"
     gene            3775..>4256
                     /locus_tag="VF686_10500"
     CDS             3775..>4256
                     /locus_tag="VF686_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8662283.1"
                     /translation="MTPSTDPATAESASAAGDPQTPQPGMEAGPARKLRVAAVGDLHV
                     GEAHDRPHRELFERVHEDADVLCLCGDLTNFGKTREVEILLEDLRACKIPMVGVLGNH
                     EHECGQPEHVTKLLCDAGVKMLTGQAYENEGVGFAGGKGFIGGFGRYMLSPFGEASIK
                     A"
BASE COUNT          720 a         1427 c         1501 g          608 t
ORIGIN      
        1 cgttcaggcg cacacagtca tggaggagag cagtcatgac ctacaccgtc tatggcgcag
       61 ccggatccgg ctcggtcccg gtcgaagccg ccctgaccct gatcggcgcg ccctatcgcg
      121 tggtcgaggc cgtgacctgg gagggcgagg cggagcgcga caaggtcgcc ccggtcaatc
      181 cgatgcggca gatcccggcc ctgatcaccc cggacggcga gacgatcacc gagagcgccg
      241 ccatcctgat ctggctggcg gaacagcatc ccgaggcggc gctgggcccc gagccctgcg
      301 atccgcggcg cgcgcaattc cttcgctgga tgaccttcat ccccgcctcg gtctattcga
      361 tgttctgggt ccgcgacgag ccgtcccggc tgggcggcga cgatccggcg gcgcagaaaa
      421 ccattcggga gcgcaccgcc gggcggatcg cggactgctg ggcgaagatg gaaagccaga
      481 tcctccccga ccgtttcctt ctgggccccg agccgacggt gctggacctc tatgtcgccg
      541 tggccagccg ctggaccccg aaccgcaagc ggtttcacga ggtcgcgccg cggatggggg
      601 cggtgatgcg ccgcgtcgac gccctgccgg agctcgaggc tttctgggcc gagcgcttcc
      661 cgttcgaggg acggtacggg agttaggcgg cctcggcggc ctcggagacg cgggcttccg
      721 gcagatcgcc ggacagagcc cggttcacca tcgccagcag gccggcctgg ttgatcggct
      781 tggccatgtg ggcgttcatg cccgaggcca gacagcggat cacgtcctcg ggcatggcgt
      841 cggcggtcat ggcgacgacg gcgatgtcgt gctcgggccc cggcagggcg cggatgcggc
      901 gcgtcgcctc cagcccgtcc atgagcggca tgcgcacgtc catcagcacc aggtcgaacg
      961 gctggccggc gaccgcgtcg atggcgtcct gtccgtcgca gacctcgacc acgtcgcagc
     1021 cgaacgcttc cagcatgatc cgggcgacct cgcggttgac cggatgatcg tcgacgacca
     1081 gcacgcgggg cgtcgcgggg gcggcggcct cgccggtttc ctcgtcgcgg atgtcgggca
     1141 tggggcgttc gctctcttcg atcgcgtcgg gggcccgcgg gaggggcagg gtgatgatga
     1201 aggtggagcc ctcgcccggc cggctgtcga cgtcgatatg gccgttcagg cggcggacgt
     1261 gccgggccac aagcgccaga ccgaggcccg ccccgcaatg gctgcggcgg atcgaggcgt
     1321 cgccctgggc gaaggcctcg aacaggtccg gcagcaaaac ggggtcgatg ccggagccgg
     1381 tgtccgagac gcggatgcag accccgtcgg acgaccggtc cagccggatg gcgacctgac
     1441 cctcgtcggt gaatttgacc gcattggcga ccatgggatg cagggtctgg cgcagcgccc
     1501 ggcggtcgca catgaagcgc gcgtttgacg gcaggcggtc gtcgatcgtc aggctcagcc
     1561 ccttggcgtc ggccgcggcc cggctgggcg cgatcgcctc gcgcaccacg gcggcaaggt
     1621 ccagcgcctc gggcgtgact gcgtcgtcgc cgcgggcgaa gtccagaata tcctcgacca
     1681 gcatcagcag accgtcgctg gactcccgga tctggcgcgc ctgacgcagg gcggtggcgt
     1741 ccagtcccgg ccggccggcc agaacgtcgg cgaagccggc gacgccgttc aggggggtgc
     1801 gcatctcatg gctcatcatg gccaggaagc gcgagcgggc ggcggcggcg tcctcggcct
     1861 tgcggcgggc ctcctgggcc gcgaccgtcc gggcgcgcag ccgcgcctcg agccggcggc
     1921 gctcggtcat gacggtggaa accggcagga cggtgaccac catggcgagc aggaacaggt
     1981 tgtagacgcc gagccgccgc agcatgggcg gaacggcttc aaggccaggg atgtcggcga
     2041 gccgggtcag atgtacgggc ccgaagccgg tcagggtcgc gaccccgctg accgccgtca
     2101 tgccgatcag ggcgatcgcc gccgtggtcg gcgacagcct gagggcgatc agcagcatgg
     2161 gcaggaagca caggaatccg agaggcgcgt cgttctgcca gaaggcgagc gtggtcacga
     2221 ccagcatcag tcccagcagt gctgtcgctt ccggccagcg cgcgcgggtc gtcccgtgga
     2281 accggtgggc ccgggccatc agcagcagga caggcgtcac cagcagcaga ccgagcagct
     2341 ccacaatgat gaaggaatag atgccgaaag cgccggccac ggtcagcggc gtctggcgga
     2401 tcagcatggt caggccgaag ccgagggccg ccgtcgcggc gaccgccggc agggcggcga
     2461 acaggccgaa ggcgaacagg cgtcggggac ggcgcatgtc cagggcggcg ccgcagaaac
     2521 gtcgggccag aacggccgcc aacacggcct cgcccaaatt catcaccgcg ttgagccaca
     2581 tcagcatgcc ggcgtcgccg cggatggcgt tgctggacag gttgatgacg agacaggccg
     2641 cgaccacggt gaccgcctgt cgccggtgca gttgcagcag ggccgccgcc atgatgccgt
     2701 tggagagcca gatcagcact gcgtcgtagg tggccacgct ccagtggccg atcagttggg
     2761 acgcggcgaa cagcagcaca taggcccagg ccggcggccc ggcgacgcgg cccgcttcat
     2821 gcctgaataa acgccaacga cccttcaaca cgagcctccc cggcccttac cctgaacagt
     2881 cgcaacgtta gggcgaatgc gtaaacaaac ccgtcacatt cgtgacgcaa gccagccccg
     2941 caggaacccg cgcccgaccg gagccgtttc cagacccgcg ccctcgccgg cgggaaatgg
     3001 agagccggac catgtcagac accgtcgccg agcccagctt ccatccgccg gaagaggcgg
     3061 tggccttcta cgaggaatcg ctgcgcctgc tgaaggaaag cggcatcccc ttcctgctgt
     3121 cggggacata cgccgtcacc gcctatacgg gcatccagcg gccgaccaag gatctggacg
     3181 tcttctgcaa gcccggcgac tacccgcgca tccttgcctt cttccaggcg cggggttacc
     3241 gcaccgacgt cgaggacgag cgctggatcg ccaaggtctg gaaggacgaa aagcatttct
     3301 tcgacgtgat cttcgccatg tccaatggca cgatcgcggt caatgacgcc tggttcgggc
     3361 ccgacaccat cgaggtctat ggtcacgagg tcacgatcac ctcgcccacg gcgctgatcc
     3421 tgtccaaggt cttcatccag gaccgctacc gctacgacgg ggcggacgtg aaccacatca
     3481 tcctcaaaca gtctggcgcc atcgactgga agagcctgct ggaccagatg gacctctatt
     3541 gggaggtgct gatgtcccac cttctgaact tccggttcgc ctatcccacc gagcggggca
     3601 acatccctgc atggctgatg cacgagctga ccgagcggct gcgcgcccag gtcgatctgc
     3661 cggcgccgcg cgtgaaggtg tgccgcggac ggctgttcag cgcgcgggac tatatctcgg
     3721 acatctcgga atggggcttc ggcgacattg tcggcaaggg gctggaggag cgccatgacc
     3781 ccatccactg atccggccac ggcggagagc gccagcgccg cgggcgaccc ccagacgccg
     3841 cagccgggca tggaagccgg gcccgcgagg aagctgcgcg tcgcggcggt cggcgacctg
     3901 catgtcggcg aggcccatga ccggccgcac cgcgaactgt tcgagcgggt tcacgaggac
     3961 gccgacgtcc tgtgcctgtg cggcgacctg accaatttcg gcaagacgcg cgaggtggag
     4021 atccttctgg aggacctgcg cgcctgcaaa atccccatgg tcggggtgct gggcaatcac
     4081 gagcatgagt gcggccagcc cgaacatgtg acgaaactgc tctgcgacgc cggggtgaag
     4141 atgctgaccg gccaggccta tgagaacgag ggcgtcggat tcgcgggcgg caagggattc
     4201 atcggcggct tcggccgcta catgctgagc ccgttcggcg aggcctcgat caaggc
//
LOCUS       DASVVN010000640         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_236131,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000640 DASVVN010000000
VERSION     DASVVN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236131"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..963
                     /locus_tag="VF686_10505"
     CDS             94..963
                     /locus_tag="VF686_10505"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8662284.1"
                     /translation="MDRVAEVADLVTVALDELLPRASGPESRLTEAMRYAALGPGKRL
                     RPFFALEAAELFDIEERPVLRAACALECVHAYSLVHDDLPAMDDDDMRRGRPTVHKAY
                     DEATAILAGDALQTAAFDILLHPDTHDDAGIRCELAARLSLASGARGMAGGQMIDLLG
                     VRDDLGGVARMQRLKTGALFTYAFEIPLIIARAREAHRQALISFAHDIGLAYQIVDDL
                     LDVEGDEDILGKAAGKDAAQGKTNFVTLLGVEAARHRVAHLADQARGHLDVFGPDAEI
                     LKASVDYVLQRRS"
     gene            1118..3028
                     /gene="dxs"
                     /locus_tag="VF686_10510"
     CDS             1118..3028
                     /gene="dxs"
                     /locus_tag="VF686_10510"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX8662285.1"
                     /translation="MPDTPLLDQVTFPADTRDLDIPQLKQLADELRAETIDAVSVTGG
                     HLGAGLGVVELTVALHHILETPKDILIWDVGHQCYPHKILTGRRDRIRTLRQPGGLSG
                     FTKRSESEYDPFGAAHASTSISAALGFAAARDQKGETNRVVAVIGDGSMSAGMAYEAM
                     NNAAETTNGQLMVILNDNDMSIAPPVGGMSAYLAKQVSGGAYRNFRRFGKSVAEHLPR
                     PFRNAARKAEEYARGMIVGGTFFEELGFYYVGPVDGHDMDNLVPILKNAAAITDRPVV
                     VHVVTQKGKGYAPAESSADKLHAVAKFDVVSGKQAKAASNAPSYTKVFGTELIKRAQL
                     DPTIVAITAAMPSGTGLDLFGQAFPTRTYDVGIAEQHAVTFAAGLAADGMKPVCAIYS
                     TFLQRGYDQVVHDVAIQKLPVRFAMDRAGLVGADGATHAGSFDIGFMGALPGMVLMAA
                     ADEADLAAMIATSLEIDDRPSAFRYPRGDGVGVEIPELAAPLEIGKGRIVREGTTVAI
                     LSLGTRLQESLKAAEALAARGVSATVADARFAKPLDADLILRLAREHECLITVEEGAM
                     GGFGAFVLQLLSDKGALDAGLKIRTLCLPDVFQDQDNPAAMYATAGLNAEHIAAAALQ
                     ALGVEATRAVRA"
     gene            3206..>3461
                     /locus_tag="VF686_10515"
     CDS             3206..>3461
                     /locus_tag="VF686_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662286.1"
                     /translation="MKLRILSVAAVLTAVVVPPAMAQQAEAAAERPFEIMRAGDTAMT
                     CPQISEEAAQLSQQLGGTPGGGILGTISGVASAGAAMLIPG"
BASE COUNT          542 a         1337 c         1073 g          509 t
ORIGIN      
        1 cggcgtcgac cgcgaggtcg agatcggcgg gaggctgttc tgaccctgct tccgccccag
       61 gccccggccg ccaactcccc ggagcagggc gtcatcgacc gcgtcgccga ggtcgccgat
      121 ctggtcaccg tcgccctcga cgagttgctg ccccgcgcca gcggccccga atcgcgactc
      181 actgaggcga tgcgctacgc cgccctcggc cccggcaagc ggctgcgccc cttcttcgcg
      241 cttgaggccg ccgagctgtt cgacatcgag gagcggccgg tgctgcgcgc cgcctgcgcc
      301 ctggaatgcg ttcacgccta cagtctcgtc cacgacgacc tgccggccat ggacgacgac
      361 gacatgcgcc gcggccggcc cacagtgcat aaggcctatg acgaggccac cgccatcctc
      421 gccggcgacg ccctgcagac cgccgccttc gacatcctgc tccaccccga cacccacgac
      481 gacgccggga tccgttgcga gctcgccgcc cggctgtcgc tggcctcggg cgcgcgcggc
      541 atggcgggcg gccagatgat cgacctgctg ggcgtccgcg acgatctcgg cggcgtcgcc
      601 cgcatgcagc ggctgaagac gggcgccctg ttcacctacg ccttcgaaat tcccctgatc
      661 atcgcccggg cccgcgaggc gcaccgtcag gcgctgatca gcttcgccca cgacatcggc
      721 ctggcctacc agatcgtcga cgaccttctg gacgtcgagg gcgacgagga catcctcggc
      781 aaggcggccg gcaaggacgc cgcccagggc aagaccaatt tcgtcaccct tctgggcgtc
      841 gaggcggcgc gtcacagggt cgcgcacctc gccgatcagg ctcgcggcca cctcgacgtc
      901 ttcggccccg acgccgaaat cctcaaggcc agtgtggact acgttcttca gcggcggtcg
      961 tagccccgcc ctgtcgttcc gggcgacacc ggcgtggccc ggtgcgaagg ctcgatcacg
     1021 aacagctcag gaatccctca ctggcgttcc ccgtgctagg cgttcatgga atagccgtat
     1081 cgtttcgccc ggttcgggcg ccgtccaagt aatatccatg cccgacaccc cgctgctcga
     1141 tcaggtcacc ttccccgccg acacccggga cctcgatatc ccgcagctga aacagctggc
     1201 cgacgaactg cgcgccgaaa ccatcgacgc cgtctccgtc accggcggcc acctcggggc
     1261 cggcctcggc gtcgtcgaac tgaccgtcgc cctgcaccac attctggaaa cgccgaagga
     1321 catcctgatc tgggacgtcg gccatcagtg ctatccgcac aagatcctga ccggccgccg
     1381 cgaccgcatc cgcaccctgc gccagcccgg cggcctgtcc ggcttcacca agcgcagcga
     1441 gagcgaatac gaccccttcg gcgccgccca cgcctcgacc tcgatcagcg ccgccctcgg
     1501 cttcgccgcc gcccgcgacc agaagggcga gaccaaccgc gtcgtggccg tcatcggcga
     1561 cggctccatg tccgccggca tggcctatga ggccatgaac aacgcggccg agacgaccaa
     1621 cggccagctg atggtcatcc tcaacgacaa cgacatgtcg atcgccccgc cggtcggcgg
     1681 catgagcgcc tatctggcca aacaggtctc gggcggcgcc taccgcaact tccgccgctt
     1741 cgggaagtca gtcgccgaac acctgccgcg cccgttccgc aacgcggctc gcaaggccga
     1801 ggaatacgcc cgcggcatga tcgtgggcgg caccttcttc gaggagctgg gcttctacta
     1861 cgtcggcccc gttgacgggc acgacatgga caacctcgtc cccatcctga agaacgccgc
     1921 cgccatcacc gaccggccgg tggtggtcca cgtcgtgacg cagaagggca agggctacgc
     1981 ccccgccgag agcagcgctg acaagctgca cgccgtcgcc aagttcgacg tcgtctccgg
     2041 caaacaggcc aaggccgcct ccaacgcccc cagctacacc aaggtgttcg ggaccgagct
     2101 gatcaagcgc gctcagctgg acccgaccat cgtcgccatc accgccgcca tgccctcggg
     2161 caccggtctg gacctgttcg gtcaggcctt cccgacccgc acctatgacg tcggcatcgc
     2221 cgaacagcac gccgtcacct tcgccgccgg tctcgccgcc gacggcatga agccggtctg
     2281 cgccatctat tccaccttcc tccagcgcgg ctacgaccag gtcgtccacg acgtggcgat
     2341 ccagaagctg ccggtgcgct tcgccatgga ccgcgccggt ctggtcggcg cggacggggc
     2401 cacccacgcg ggcagtttcg acatcggctt catgggcgcg cttccgggca tggtcctgat
     2461 ggccgccgcc gacgaggccg atctggccgc catgatcgcc accagcctcg agatcgacga
     2521 ccgcccctcc gccttccgct atccgcgcgg cgacggcgtc ggggtcgaga ttccggaact
     2581 ggccgccccg ctcgagatcg gcaagggccg catcgtgcgc gagggcacga ccgtcgccat
     2641 cctgtcgctg ggcacccgcc ttcaggaatc gctcaaggcc gccgaggccc tggccgcccg
     2701 cggcgtctcg gccaccgtcg ccgacgcccg cttcgccaag cctctcgacg ccgacctgat
     2761 cctgcgcctc gcccgcgagc acgaatgcct gatcacggtg gaggaggggg ccatgggcgg
     2821 cttcggcgcc ttcgtgctcc agctgctgtc cgacaagggc gcgctcgacg ccggtctgaa
     2881 gatccgcacc ctgtgcctgc ccgacgtctt ccaggatcag gacaacccgg ccgccatgta
     2941 cgccacggcc ggcctgaacg ccgaacacat cgccgccgcc gcgcttcagg cgctgggcgt
     3001 cgaggcgaca agggcggtcc gggcctgatc cgcctctccc tcccctttat ggggagggat
     3061 gtcggcgcgg agcgacgaca gggtggggta aggttgaagt tccccacccg gtcgcttcgc
     3121 gaccaccctc cccatgaagg ggagggagaa cgcaaccccc gccctcccgg cccgttctgt
     3181 cgcggggctt tgagggttga gtacaatgaa actgcgtatc ctgtccgtcg ccgccgtcct
     3241 tacggcggtc gttgtcccgc ccgccatggc ccaacaggca gaggctgcgg ccgagcgtcc
     3301 cttcgagatc atgcgcgccg gcgacaccgc catgacctgc ccgcagattt cggaagaggc
     3361 cgcacaactc agccagcaac tgggcggcac gcccggcggc ggcatcctcg gcacgatctc
     3421 cggcgtggcc tcggccggcg cagccatgct gatccccggc g
//
LOCUS       DASVVN010000641         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_130823,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000641 DASVVN010000000
VERSION     DASVVN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130823"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1400
                     /locus_tag="VF686_10520"
     CDS             114..1400
                     /locus_tag="VF686_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8662287.1"
                     /translation="MIRRLVLATLLFAGLAAPAAGQGAGRAYAVQGECGGRPRTLVVM
                     ADGSCMARVWQRTGDEGPRMPRGLLELPGGDWLVTDLGSWDPGRGAVWRMSPQPGGGV
                     RWVRLIRGLSMPHAIARGPDGRIYVAEMSRILAFDPARPDAAVAVVEGLPDNRLHENR
                     HPLSSFVFAGDGALLVNVGAPSDRCLTPAGAPIRDARGQCAEAAEQAMVRRYASTGPG
                     RWSRDWTAFASGLRNSVAMVRHPSGAVFQAENSVDLNTPDRPFDEINRLTAGGHYGWP
                     YCTDMATAQPGWTARQARCGERSAPVSLLPPHAAPLAMLYYDGAMFPELRGRMLMSWH
                     GYRRAAGRVVAIETDASGAPLTDRRAHYAIYPRGSRSYPTEAPAPRAKILTPGWNARP
                     GRHPQGSPAGLAVARDGSIWIADDRSAAILRIARSD"
     gene            complement(1397..2629)
                     /locus_tag="VF686_10525"
     CDS             complement(1397..2629)
                     /locus_tag="VF686_10525"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX8662288.1"
                     /translation="MTQTAPKKVVLAYSGGLDTSIILKWLQTEYGAEVVTFTADLGQG
                     EELAPAREKALKMGIKPENIFIDDLREEFVRDYVFPMFRANALYEGQYLLGTSIARPL
                     IAKRQIEIARMVGADAVCHGATGKGNDQVRFELGYYALEPDIRVIAPWREWAFKSREH
                     LLDFAEKHQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPSVEAPEFVHQRTLSPED
                     APDVATVITIGFEKGDPVSINGEAMSPATLLTALNQYGHDNGIGRLDLVENRFVGMKS
                     RGVYETPGGTILLAAHRGIESITLDGGAMHLKDELAVKYAHLVYNGFWFSPEREMLQA
                     AIDHSQQKVSGTVRVKLYKGNATVIGRESAESLYDQELVTFEDGAVAYDQKDAAGFIK
                     LNALRLRVLGKRDARDRG"
BASE COUNT          394 a          976 c         1056 g          460 t
ORIGIN      
        1 ggagaagggg ccgatgctcc ttatcacagg gcggcgtcaa cggcgccgcc gggagagaga
       61 tttgtcgaaa gcccggtccg ggctagcgtg tccgggccct caggatttcg ccgatgatcc
      121 gccgtctcgt cctcgccacc ctgcttttcg cgggtctcgc cgctccggcc gccggccagg
      181 gcgcgggccg ggcctatgcg gtccaaggcg agtgcggcgg ccgcccccga accctggtcg
      241 ttatggccga cggttcctgc atggcccggg tctggcaacg gaccggcgac gaggggccgc
      301 ggatgccgcg cggcctgctg gaactccccg gcggcgactg gctggtcacc gatctgggat
      361 cgtgggaccc cggccggggc gcggtgtggc ggatgtcgcc gcaacccggc ggcggggtgc
      421 gatgggtccg actgatccgc ggcctgtcca tgccccacgc gatcgcacgc ggccccgacg
      481 gacggattta cgtcgccgag atgagccgca tcctcgcctt cgacccggcc cggccggacg
      541 cggctgtggc cgtggtcgag ggcctgccgg acaaccgcct gcatgagaac cggcacccgc
      601 tgtccagctt tgtcttcgcc ggcgacgggg ccctgctggt caatgtcggg gctccgtcgg
      661 accgatgcct gacaccggcg ggcgcgccga tcagggacgc gcgcgggcag tgcgccgagg
      721 cggccgagca ggcgatggtg cggcgctacg cctccacggg cccgggccga tggagccggg
      781 actggacggc gttcgcctcg ggcctgcgca actcggtggc catggtccgg cacccgtcgg
      841 gcgcggtgtt ccaggcggaa aattctgtcg atctgaacac gccggaccgg ccgttcgacg
      901 agatcaatcg gctgacggcg ggcggccact acggctggcc ctattgcacc gacatggcga
      961 cggcccagcc ggggtggacg gcgcggcagg cccgctgcgg cgaacggtcc gcgccggttt
     1021 cgctgctgcc gccccacgcc gcgccgctgg ccatgctcta ttacgacggg gcgatgttcc
     1081 cggagctgcg cggccggatg ctgatgagct ggcacggtta tcgccgcgcc gcgggccggg
     1141 tcgtggccat cgaaaccgac gcgtccggcg cgcccctgac cgaccgccgg gcccactacg
     1201 ccatctatcc acgcgggtcc cggtcctatc cgaccgaagc gccggcgccg cgcgcgaaaa
     1261 tcctgacccc gggctggaac gcccggcccg gccgccatcc gcagggctcg ccggcgggcc
     1321 tcgcggtcgc ccgcgacggc tcgatctgga tcgccgacga ccgcagcgcg gccatcctcc
     1381 ggatcgcccg gagcgactag ccccggtcgc gcgcgtcgcg cttgcccaga acccgcaggc
     1441 gcagggcgtt cagcttgatg aagccggcgg cgtccttctg gtcataggcc acggcgccgt
     1501 cctcgaaggt gaccagttcc tgatcgtaca ggctctcggc gctctcgcgg ccgatgacgg
     1561 tggcgttgcc cttgtagagc ttgacccgga ccgtgcccga caccttctgc tggctgtggt
     1621 cgatggcggc ctggagcatc tcgcgctcgg gcgagaacca gaagccgttg tagactaggt
     1681 gggcgtattt gaccgccagt tcgtccttca gatgcatggc gccgccgtcg agcgtgatcg
     1741 actcgatgcc gcggtgggcg gccagcagga tggtcccgcc cggggtctcg tagacgccgc
     1801 gcgacttcat gccgacgaag cggttctcga ccagatcaag gcggccgatc ccgttgtcgt
     1861 ggccgtactg gttcagggcg gtcagcaggg ttgcggggct catcgcctcg ccgttgatcg
     1921 agacgggatc gcccttctcg aagccgatgg tgatgacggt ggcgacgtcc ggcgcgtcct
     1981 cgggcgacag ggtgcgctgg tgcacgaact cgggggcctc gaccgacggg tcctccagca
     2041 ccttgccctc ggaggagctg tgcaggaggt tggcgtcgac gctgaacggc gcctcgccgc
     2101 gcttgtcctt ggcgatcggg atctggtgct tctcggcgaa gtccagcagg tgttcgcgcg
     2161 acttgaacgc ccattcgcgc cacggggcga tgacgcggat gtcgggctcg agcgcgtagt
     2221 agccgagctc gaaccggacc tgatcattgc ccttgccggt ggcgccgtga cagacggcgt
     2281 cggcgccgac catgcgggcg atctcgatct ggcgtttggc gatcagcgga cgggcgatcg
     2341 aggtgccgag cagatactgc ccctcgtaca gggcgttggc ccggaacatg gggaagacgt
     2401 agtcgcgcac gaactcctcg cgcagatcgt cgataaagat gttttcgggc ttgatgccca
     2461 tcttcagcgc cttctcgcgc gccggcgcca gttcctcgcc ctgccccaga tcggcggtga
     2521 aggtcacgac ctcggcgccg tattcggtct gcagccattt caggatgatc gaggtgtcga
     2581 ggccgccgga ataggcgaga acaaccttct tgggcgcggt ctgggtcatg cggaatcctt
     2641 cggagaacgc ggggaggcgt tcggcggcgt tgcgcgcgct taaaggacca gacgccctgc
     2701 gatgcaaggg cgggattgcg gcggggtcag ccccagggac ccgagcggct gaccggcgtc
     2761 gacggcacgc tggtagtcgt gggccgggac gcggccgtgg caagggtggg acgacggggg
     2821 gccagaccca tcttcgcgcc cagctccatc agggccctga cgcggttgcc ggtggccgga
     2881 tgggtc
//
LOCUS       DASVVN010000642         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_471824,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000642 DASVVN010000000
VERSION     DASVVN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471824"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF686_10530"
     CDS             <1..275
                     /locus_tag="VF686_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8662289.1"
                     /translation="RKWRVLERRLAQLGIRFSPEPDRWVGSRPTLPDYLPAIGRLESE
                     PRILYAFGHQHLGLTMAAITAELTASIAEDRAPAIDLTPFRIERFS"
     gene            complement(318..>2455)
                     /locus_tag="VF686_10535"
     CDS             complement(318..>2455)
                     /locus_tag="VF686_10535"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HEX8662290.1"
                     /translation="TGLSVEGTDTTSHTEYLHTDEQGRPLSPAYRVVTTDKQSRYEIE
                     KRIYTDPDSQSLVIRTSIRALEGDITPYLLLEPHMANTGIGDRGDVEDAGGEGELASM
                     NLTGFGALHASEGDVHLTLLGLGRFTARSVGFVGQSDGLTDLADRRLDQSYSATGDNT
                     GNIMLTGALPSIREGQAASHDFVVGFGSTRDEATSAALVPFVADLDEVLARFNGEGDR
                     VGWEDYLASLADLPRIAEQSMDGGKLAYASALMLKVQEDRTHAGALIASLSNPWGDTV
                     DATESSTGYKAVWPRDFYQVAMALMALGDAETPVAAFRYLPQVQVRADTPGNTGATGW
                     FLQKTHVDGQIEWVGVQLDQTAMPIMLGWKLWKAGRIDDAELAAMYARMIKPAADFLV
                     DGGKAGILWNDRQITPPWTQQERWEEQEGFSPSTTAAVITGLTVAADIARASGDPASA
                     SRYQAAADDYASKIEARMFTTAGEYGDGRYFLRLSRNEDPNDKLPLGENNGQPAMPED
                     RIIDGGFLELVRYGVRAADAPSITASLPEYDDQMREARFRVRYDLNGVPGFRRYGNDG
                     YGENTETGGNYGVGGMTPGQRGRVWPIFTGERGHYELAALLAHPSADQDWNTAQVNLI
                     RQTYVRGMESFANDGLLLPEQVWDGVGDDTAHGYRPGEGTNSATPLAWSHAEYIKLLR
                     SLSDRAVWDRYDPVEARYAPSAGRAPGGR"
BASE COUNT          368 a          785 c          880 g          422 t
ORIGIN      
        1 cccgcaaatg gcgcgtgctg gagcggcggc tggcgcagct cgggatccgt ttctcgcccg
       61 agcccgaccg ctgggtcggg tcgcggccga ccctgcccga ctatctgcct gcgatcggcc
      121 ggctggagag cgaacctcgc atcctctacg ccttcggtca tcagcacctg ggcctgacca
      181 tggcggcgat caccgccgaa ctgacggcgt ccatcgccga ggaccgcgcg ccggcaatcg
      241 acctgacgcc gttccgaatc gaacggttca gctagccccc cgctgggtac gcgcccgcga
      301 ccaccgaggc tggaaggtca cctgccgccg ggagcccgcc cggcagacgg cgcgtaacgc
      361 gcctcgaccg ggtcgtagcg gtcccagacc gcgcggtcgg atagcgagcg cagcagcttg
      421 atgtattcgg cgtggctcca ggccagcggg gtggcggagt tggtgccctc gcccggacgg
      481 tagccgtgcg cggtgtcgtc gccgacgccg tcccagacct gttccggcag cagcaggccg
      541 tcgttggcga aggactccat gccgcgcaca taggtctggc ggatgagatt cacctgtgcc
      601 gtgttccagt cctgatcggc ggacggatgg gccagcaggg ccgcaagttc gtaatgaccg
      661 cgctcgccgg tgaagatggg ccagacccgg ccgcgctggc cgggggtcat cccgccgacg
      721 ccgtagttgc cgccggtctc ggtattctcg ccgtagccgt cgttgccgta gcggcggaag
      781 cccgggacgc cgttcaggtc gtagcggacc cggaaccgcg cctcgcgcat ctggtcgtca
      841 tattcgggca ggctggcggt gatcgagggg gcgtcggccg cgcgcacgcc gtaacgcacc
      901 agttcgagga agccgccgtc gatgatgcga tcctcgggca tggcgggttg gccgttgttc
      961 tcgcccagcg gtagtttgtc gttgggatcc tcgttgcggc tgaggcgcag gaaatagcgg
     1021 ccgtcgccgt attcgccggc ggtggtgaac atccgcgcct cgatcttcga cgcatagtcg
     1081 tcggcggcgg cctggtagcg ggaggcggaa gccgggtcgc ccgaagcacg ggcgatgtcg
     1141 gcggccacgg taagcccggt gatgacggcg gcggtcgtcg agggggaaaa gccttcctgc
     1201 tcttcccagc gctcctgctg ggtccagggc ggggtgatct ggcggtcgtt ccacaggatg
     1261 ccggccttgc cgccgtcgac gaggaagtcg gccgccggct tgatcatccg ggcgtacatg
     1321 gcggccagtt cggcgtcgtc aatgcggccg gctttccaga gtttccagcc cagcatgatc
     1381 ggcatggcgg tctggtcgag ctggaccccg acccattcga tctgaccgtc gacatgggtc
     1441 ttctggagga accagcccgt ggcgccggtg ttccccggcg tgtcggcgcg gacctggacc
     1501 tgtggcaggt agcggaaggc ggcgaccggg gtctcggcgt cgccgagcgc catcagggcc
     1561 atggccacct ggtagaagtc acgcggccag acggccttgt agcccgtcga ggattcggtg
     1621 gcgtcgaccg tgtcgcccca cgggttggac agggaggcga tcagggcgcc ggcgtgggtg
     1681 cggtcctcct gcaccttgag catcagggct gaggcgtaag cgagcttgcc gccgtccatg
     1741 gactgttcgg cgatgcgggg caggtcggcg agggaggcga ggtagtcttc ccagccgacg
     1801 cggtcgccct cgccgttgaa acgggcgagg acctcgtcga ggtcggcgac gaaaggtacg
     1861 agcgccgcgc tggtggcttc gtcgcgggtc gaaccaaagc caacaacaaa gtcatgcgaa
     1921 gcggcctgac cttcacggat cgagggcagg gcgccggtca acatgatgtt gccagtgttg
     1981 tcgccggtgg cgctgtacga ttggtccaga cgacgatccg cgaggtccgt caaaccatct
     2041 gactggccta caaatcccac gctgcgtgcg gtgaaccgac caagtccgag aagtgtgagg
     2101 tgcacgtcgc cctccgaggc atggagagcc ccgaagccgg tcagattcat ggaggcgagt
     2161 tcgccctcgc caccagcatc ttcaacgtcg ccgcggtcgc cgatgccggt gttggccatg
     2221 tgcggctcga gcagcaggta gggcgtgata tcgccctcca acgcccggat ggaggtgcga
     2281 atcaccagcg actgactatc cgggtcggta tagatccgct tctcgatctc gtagcggctc
     2341 tgtttgtccg tggtgacgac ccgatacgcc ggtgacagcg ggcggccctg ttcgtccgtg
     2401 tgcagatatt cggtgtggct ggtggtgtcc gtgccctcta ccgaaaggcc cgtct
//
LOCUS       DASVVN010000643         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_51049, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000643 DASVVN010000000
VERSION     DASVVN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51049"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF686_10540"
     CDS             complement(<1..355)
                     /locus_tag="VF686_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8662291.1"
                     /translation="MTDHIRTELADGVLTVTLARPEKKNAITQAMYAALSDATEQART
                     DDAVRVLLFRGEGDSFSAGNDIADFIAIGSQTGEPLDMSVFHFLKALAELDKPAVAAV
                     RGRAVGIGLTLLLHCD"
     gene            complement(352..1443)
                     /locus_tag="VF686_10545"
     CDS             complement(352..1443)
                     /locus_tag="VF686_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8662292.1"
                     /translation="MADSVVTAEIGEAASGTRGLTYPFGDPPGFGEAVAVAPGVLWMR
                     LSMPIALNHINVYAVEDGDGWALIDTGLNIPDSRERWDALLAGPLGGKPVTRVICTHM
                     HPDHIGLAGWLCQRFGVGLTMTRLEYVTGRMLLADTGQPAPEAGAVHYRAAGWDEAQI
                     ETYRREYGQFGRAVSPLPSGYTRMREGDRISIGGQDWRVVVGEGHSPEHACLWRETDG
                     VVLGGDQILPKISSNVSVWPTEPEADPLGDWLASLERMKTIFPDDVLVLPSHGEPFRG
                     VQARLDALIRGHTTALKRLEKTLREPKRAVDVFGALFARPVGDGVRGMATGEALAHLN
                     YLLQRGRVSRTRDADGVDWWTMINEGEAA"
BASE COUNT          258 a          659 c          532 g          259 t
ORIGIN      
        1 tgtcgcagtg aagcagcagg gtcaggccga tgccgaccgc ccggccccgc accgccgcca
       61 ccgccggctt gtccagctcg gccagcgcct tcaggaagtg gaacaccgac atgtccagcg
      121 gctcgcccgt ctgcgatccg atggcgatga agtcggcgat gtcgttgccc gccgagaagc
      181 tgtccccctc gccacggaac agcagcaccc ggaccgcgtc gtccgtgcgg gcctgctcgg
      241 tcgcgtcgga aagggccgcg tacatggcct gggtgatggc gttcttcttc tcgggccgcg
      301 ccaaggtgac agtcaggacg ccgtcggcca gttcggtgcg aatatggtcg gtcacgccgc
      361 ctctccctcg ttgatcatgg tccaccagtc gacgccgtcg gcgtcccgcg tccggctgac
      421 ccggccgcgt tgcagcagat aattcagatg cgccagcgcc tcgccggtcg ccatgccacg
      481 cacgccgtcg ccgacaggcc gggcgaacag ggcgccgaag acatcgaccg cccgtttcgg
      541 ctcccgcagc gtcttctcca gccgcttcag cgccgtcgtg tgaccgcgga tcagggcgtc
      601 cagccgcgcc tgcacgcccc ggaatggctc gccgtgcgat ggcagcacca gcacatcgtc
      661 cgggaagatg gtcttcatcc gctccagcga ggccagccag tcgcccagcg ggtcggcctc
      721 cggctccgtc ggccagaccg acacgttcga ggaaatcttc ggcaggatct ggtcgccgcc
      781 cagcaccacc ccatccgtct cgcgccacag gcaggcgtgt tccgggctgt gcccctcgcc
      841 gaccaccacg cgccagtcct ggccgccgat gctgatccgg tcgccctcgc gcatccgcgt
      901 atagcccgac ggcaggggcg agacggcccg cccgaactgg ccgtattccc ggcggtatgt
      961 ctcgatctgc gcctcgtccc agcccgcggc gcgatagtgg accgcgcccg cctccggcgc
     1021 cggctgaccc gtatcggcca gcagcatccg cccggtcaca tattccagcc gcgtcatggt
     1081 caggcctacg ccgaaccgct ggcacagcca gcccgccagt ccgatgtggt cggggtgcat
     1141 atgggtgcag atcacccgcg tcaccggctt cccgcccagc gggcccgcca gcagggcgtc
     1201 ccagcgctcg cgcgaatccg gaatgttcag ccccgtgtcg atcagggccc agccgtcgcc
     1261 gtcctcgacc gcatagacat tgatatggtt gagggcgatc ggcatcgaca gccgcatcca
     1321 cagcaccccg ggcgcgaccg cgacggcctc gccaaacccg ggcgggtcgc cgaacggata
     1381 ggtcaggccg cgcgttccag acgcggcttc cccgatttcc gccgtcacga cgctgtccgc
     1441 caagtccgtc tccaataaaa actaacgcgc ctggattaag ccaagcgggg tcgcgcgtcc
     1501 agcgccgtcg cggtctcgcc ttgaccaggc cacaactgcc cgccaagggt gcgcccacgg
     1561 ctttccccac ccggagtttc atcgtgttcc atagaaagtt gctcggcctg gccggcggcg
     1621 ccatcgccct cgctctcgca gcggcccagc cggccctcgc ccaggacccg cagtcggagt
     1681 ccgaccgcgc ctccggaacc attcaggg
//
LOCUS       DASVVN010000644         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_208272,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000644 DASVVN010000000
VERSION     DASVVN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208272"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /gene="grpE"
                     /locus_tag="VF686_10550"
     CDS             complement(<1..462)
                     /gene="grpE"
                     /locus_tag="VF686_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX8662293.1"
                     /translation="MSDIHDEFDDEAEANAEAGLDAHPGDDDDMGPIDALIAERDQWK
                     DRALRAVAEAENTKKRAELSNNDARAYAIQKFARDLLGVADNLERALQAAPKNADAGE
                     AGLVTGLELTQKSLLQAFESNNLKRVAPEPGEAFDPHLHQAMMEQPSDIIQG"
     gene            complement(459..1517)
                     /gene="hrcA"
                     /locus_tag="VF686_10555"
     CDS             complement(459..1517)
                     /gene="hrcA"
                     /locus_tag="VF686_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX8662294.1"
                     /translation="METGSLTGLDQRARDIFRRIVETYLETGDPVGSRTLSMTGVALS
                     PASIRNTMQDLTMAGLLGAPHTSAGRIPTHAGLRLFVDGLLEIGDIGAEERREIDARL
                     AGRGRGFEEALNEASSLLSGLAGGASIVASPIREAGVKHVEFVALGHDQALAVLVGDD
                     GSVENRLMPLKAGVTPSTLTEASNFLNARLKGRPLHEARTEMRTELDAARRDLDAAAA
                     RLVEDGLAAWSGGAERERSLIVRGRANLLHDRETAEDLEKVRILFDDLEQKEQLIGLL
                     DGVSTAQGVRIFIGAETRLFSLSGSAVIAAPYMTGRQRVLGAIGVIGPARLNYARIIP
                     LVDYTARVLGRMLDGQET"
     gene            complement(1544..2119)
                     /locus_tag="VF686_10560"
     CDS             complement(1544..2119)
                     /locus_tag="VF686_10560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8662295.1"
                     /translation="MSLSPASSALAPSVRLRPATLDDAALLAQWDREPHVIECSTDDP
                     GADVAFGGIEWREELADQSEVSFYRIAEADEDGGWRPIGVMQICDPHLEPTHYWGEIE
                     PNLRAMDIWIGPKDALGRGYGTQMMTLTVDEAFADPAVTAIVIDPLNTNTEAHRFYQR
                     LGFEVVGRRMFDEDDCLVHRLTRAAWEGRKA"
     gene            2165..2881
                     /gene="rph"
                     /locus_tag="VF686_10565"
     CDS             2165..2881
                     /gene="rph"
                     /locus_tag="VF686_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX8662296.1"
                     /translation="MRPSDRTPEQLRTVTLETGVNRYAEGSCLVTFGHTKVLVTASVE
                     ENVPGWMRNKGSGWVTAEYGMLPRATHTRGRREAASGKQTGRTQEIQRLIGRSLRAVV
                     DLKLLGERQVVLDCDVIQADGGTRTAAITGAWVAMASAFDYLRAEGVLKTDPILDQVA
                     AVSCGICSDQPVLDLDYEEDSTAEADSNFVLTGAGGIVEIQATGEKRGFSRAEFDRLF
                     QLAEGGCTELFALQRAALGR"
     gene            2950..3306
                     /locus_tag="VF686_10570"
     CDS             2950..3306
                     /locus_tag="VF686_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662297.1"
                     /translation="MRLPILLTAAALLLSACQPPAEAPAAPAPAEAAQDPVMPADAPP
                     QEEAIAPTEASSTSCTLDQGSDAAIELASRCTRVSPASHPPCNPDNPCQLIQDEIDRS
                     CAMYKAGETKPAECTA"
     gene            3363..4505
                     /locus_tag="VF686_10575"
     CDS             3363..4505
                     /locus_tag="VF686_10575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8662298.1"
                     /translation="MTPVQTPPDLRALTGLRFLAALWVVLYTAWPHLDVGWVPVAVTK
                     GYLGVEVFFVLSGFILSHVYLEAAGTKRYSYGGFLWARVARVYPLHLLTLFGMVALGI
                     AGTAAGLSIDGSLTDWRSLPAHLTMTHAWGLAPVSAFNHPSWSISAEWFAYLTFPAYA
                     FVAWRLRDRPLLAVLGAVTLALAIYAAFQPLAGFSLTEATFRWGALRIAPCFALGCAL
                     YLVYRRGPIPYAGPVAVISGLALLASASLGLWDPVTVLLAGAMILGLGSLDNARAGLM
                     GSKASVYLGEISYSIYMVCAPVLLVMTNVAARLTGADDKRFHIIVWLAVVAAIPVAAM
                     GTYHLVERPCRKALRGFADRRRARSTATKPHTDSPERVLQPTKTIV"
     gene            4541..5077
                     /locus_tag="VF686_10580"
     CDS             4541..5077
                     /locus_tag="VF686_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX8662299.1"
                     /translation="MMKRLTAAAVAATLGFSALGYAPVAKADEPYWQCVTFARMFSGI
                     NIFGDALTWWRQAQGKFRTGGRPETGAVLAFQPSGRMSRGHVAVVSDILTDRVIRVTH
                     ANWGGSRGRVEENVTVVDVSSSGDWSAVKVWYNPIHDLGTSVYPTYGFIYKAPAAATG
                     PTVESVLMASADAPSGQQ"
BASE COUNT          760 a         1796 c         1773 g          883 t
ORIGIN      
        1 gccctgaatg atgtcggacg gctgttccat catcgcctga tgcagatgcg gatcgaaggc
       61 ctcgccgggc tccggggcga cgcgtttcag attgttggat tcaaaggcct gcagcagcga
      121 cttctgggtc agctcgaggc cggtgacgag cccggcctcg ccggcgtcgg cattcttcgg
      181 ggcggcctga agcgcgcgtt ccagattgtc ggccacgcca agcaggtcgc gggcgaattt
      241 ctggatcgcg taggcgcggg cgtcgttgtt cgacagctcg gcccgcttct tcgtgttctc
      301 ggcctcggcg acggcgcgca gagcgcggtc cttccactgg tcgcgctcgg cgatcagggc
      361 gtcaatcggg cccatgtcgt cgtcgtcgcc cgggtgggcg tccagaccgg cctcggcgtt
      421 ggcctcggct tcgtcgtcaa actcgtcgtg aatgtcgctc aagtctcttg tccgtccagc
      481 attcgcccca gcacccgggc ggtatagtcc accaacggta tgatgcgggc atagttcagt
      541 cgcgcgggtc cgatcacgcc gatggcgccc agaacccgct gtcggcccgt catatagggc
      601 gccgcgatca ccgcggaacc cgaaagtgag aacaaccgcg tttccgcgcc gatgaagatg
      661 cgcacgccct gagccgtcga caccccgtcc agcaggccga tcagctgttc cttctgctcc
      721 agatcgtcga acaggatgcg gaccttctcc agatcctcgg ccgtctcgcg gtcatgcagc
      781 agattggcgc ggccgcgcac gatcagggac cgttcgcgct cggccccgcc ggaccaggcg
      841 gccagaccgt cctcgaccag ccttgccgcc gcagcgtcca gatcgcggcg ggcggcgtcc
      901 agctcggtgc gcatctcggt gcgcgcctcg tgcagggggc gacccttgag ccgggcgttg
      961 aggaagttcg aggcctcggt caaggtcgag ggcgtgaccc cggccttcag cggcatcagc
     1021 cggttctcga ccgaaccgtc gtcgccgacc agcacggcca gcgcctgatc gtggccgagg
     1081 gcgacgaact cgacgtgttt gacccccgcc tcgcggatcg ggctggcgac gatgctggcc
     1141 ccgccggcga gaccggacag caggctggag gcctcgttca gcgcctcctc gaagccgcgc
     1201 ccccggcctg cgaggcgggc gtcgatctcg cgccgctcct cggccccgat gtcgccgatc
     1261 tccagcaggc cgtcgacgaa caggcgcagg cccgcgtggg tcggtatccg gcccgccgag
     1321 gtgtgggggg cgcccagcag gcccgccatg gtcagatcct gcatggtgtt gcggatcgag
     1381 gccggcgaca gggcaacgcc ggtcatggac agggtgcgcg agccgacggg atcgccggtc
     1441 tccagatagg tctcgacgat gcggcggaag atatcacggg cgcgctggtc cagaccggtc
     1501 aggctgccgg tctccatcgc cgtcgaggtc agcagggtcg gtttcatgcc ttgcggcctt
     1561 cccacgccgc gcgcgtcagc cgatgcacca gacaatcgtc ctcatcgaac atgcgccggc
     1621 ccacgacctc gaagccgagg cgttgataga agcggtgggc ctcagtgttg gtgttcagcg
     1681 gatcgatgac gatggccgtt accgccggat cggcgaaggc ctcgtcgacg gtgagcgtca
     1741 tcatctgcgt gccgtagccg cgaccgagcg cgtccttcgg accgatccag atgtccatgg
     1801 cgcgcaggtt gggttcgatc tcgccccagt aatgggtcgg ttccagatgc gggtcgcaga
     1861 tctgcatcac cccgatgggg cgccaacctc catcctcgtc tgcctcggcg atgcggtaga
     1921 agctgacctc cgactggtcg gccagctcct cgcgccattc gatgccgccg aaggcgacat
     1981 cggccccggg atcgtcggtc gagcattcga tgacatgcgg ctcgcggtcc cactgcgcca
     2041 gcagggcggc gtcgtccagc gtggccgggc gcaggcgcac cgaaggcgcg agggcggaag
     2101 atgcggggga caggctcacg gtttcaggat aagcaccgca gccgccgctt caaggaatac
     2161 cgtcatgcgc ccgtccgacc gcacgcccga acagctccgc accgtcacgc tggagaccgg
     2221 cgtgaaccgc tacgccgagg gttcgtgcct ggtgaccttc ggccatacca aggtgctggt
     2281 gacggcctcg gtcgaggaga acgtccccgg ctggatgcgc aacaaggggt ccggctgggt
     2341 cacggcggaa tacggaatgc tgcctcgggc cacccacacg cgcggccggc gcgaggccgc
     2401 cagcggcaaa cagacgggcc ggacgcagga gatccagcgg ctgatcggcc gttcgctgcg
     2461 cgccgtggtc gatctgaagc tcctgggcga gcggcaggtg gtgctggact gcgacgtcat
     2521 ccaggcggac ggcggcacgc gcacggcggc gatcaccggc gcctgggtcg ccatggccag
     2581 cgcattcgac tatctgcgcg ccgagggggt gctgaagacg gaccccattc tggatcaggt
     2641 cgcggccgtc tcatgcggca tctgtagcga ccagccggtg ctggacctcg actatgagga
     2701 ggattccacc gccgaggccg attccaactt cgtcctgacc ggcgcggggg ggatcgtgga
     2761 aatccaggcc acgggcgaga agcgcggttt ctcgcgcgcc gagttcgacc ggctgttcca
     2821 gctggccgag ggcggctgca ccgaactgtt cgcgctgcag cgggcggctc tgggccgctg
     2881 agtgtccagg gtttcatcgt tcgccgaaaa gaggcatcgt ccgcaccttc atcgtgggga
     2941 gacgatccga tgcgcctgcc gatcctgctg accgccgccg ccctgctgct gagtgcctgc
     3001 cagcctccgg ccgaagcccc cgcggccccg gcgccggccg aagccgcgca ggatccggtc
     3061 atgccggccg acgccccgcc gcaggaagag gccatcgccc cgaccgaggc ctcgtcaacg
     3121 tcctgcacgc tcgatcaggg gtcggacgcc gcgatcgaac tggccagccg ctgcacccgc
     3181 gtcagcccgg cttcgcaccc gccctgcaac cccgacaacc cctgccagct gatccaggac
     3241 gagatcgacc ggtcctgcgc gatgtacaag gcaggcgaga cgaagccggc ggaatgcacc
     3301 gcctgaggcc ggtctccggg gcttaatcgt tttccaaggc tgttgcggca gtctgccggg
     3361 tcatgacccc cgttcagacg ccccccgacc tgcgcgccct caccggcctg cgcttcctgg
     3421 ccgccctgtg ggtggtgctc tatacggcct ggccgcatct ggacgtcggc tgggtcccgg
     3481 tggcggtgac gaagggctac ctcggcgtcg aggtcttctt cgtgctgtcc ggcttcatcc
     3541 tcagccacgt ttatctcgag gcggcgggga cgaagcggta ttcgtacggc ggcttcctgt
     3601 gggcccgggt cgcgagggtc tatccgctgc atctgctcac cctgttcggc atggtcgcgc
     3661 tgggcatagc gggcacggcg gcggggctga gcatcgacgg cagcctgacc gactggcgct
     3721 ccctgccggc ccatctgacc atgacccacg cctgggggtt ggcgcccgtc tcggccttca
     3781 accatccgtc ctggtcgatt tcggcggagt ggttcgccta tctgaccttc ccggcctacg
     3841 cattcgtggc gtggcggctg cgcgaccggc cgctcttggc cgttctgggc gccgtgacgt
     3901 tggcgctggc gatctatgcc gccttccagc cgctggcggg cttctccctg acggaggcga
     3961 ccttccgatg gggcgcgctg aggatcgcgc cgtgtttcgc gctcggctgc gccctctacc
     4021 tcgtctatcg gcgcggcccg atcccgtatg cggggccggt cgccgtgatc agcggtctcg
     4081 ccctgctggc cagcgcctcg ctgggtctgt gggacccggt caccgtactc ctcgcggggg
     4141 cgatgatcct cggcctcggt tcgctggaca atgcccgcgc ggggctgatg gggtcgaagg
     4201 cgtcggtcta tctgggggag atcagctatt cgatctacat ggtctgcgcg ccggtgcttc
     4261 tggtcatgac caatgtcgcg gcgcggctga ccggggcgga cgacaagcgg tttcacatca
     4321 ttgtgtggct ggctgtggtg gcggcgatcc cggtcgcggc catggggacc taccaccttg
     4381 tcgaacgccc gtgccgcaag gctttgaggg gattcgcgga tcggcggcgg gcgcggtcaa
     4441 ccgcgacaaa accgcacacg gattcgcctg aaagggttct tcaacccacc aaaactatcg
     4501 tctaaggctt ggtcttcgga cgtcggggaa acgccacagc atgatgaaac ggctcacagc
     4561 cgccgcggtt gcggcgaccc tcggattttc tgcgctggga tacgcccccg tcgcgaaggc
     4621 cgacgagccg tactggcagt gcgtgacctt cgcccgcatg ttctcgggca tcaacatctt
     4681 cggcgacgcc ctgacgtggt ggcggcaggc ccagggcaag ttccgcaccg gcggccgccc
     4741 cgagaccggc gccgtgctgg ccttccagcc cagcggccgg atgagccgcg gccacgtcgc
     4801 cgtcgtctcg gacatcctga ccgaccgcgt catccgcgtg acccacgcca actggggcgg
     4861 cagccgcggc cgggtcgagg aaaacgtcac cgtcgtcgac gtttcgtcct cgggtgattg
     4921 gagtgcggtc aaggtctggt acaacccgat ccatgatctg ggcacctccg tctatccgac
     4981 ctacggcttc atctacaaag ccccggccgc cgcgaccggc ccaacggtcg agagcgttct
     5041 catggcctcg gccgacgccc cttccggcca gcagtaaccc caccgtccac accggatttc
     5101 cggcgtgatc cgcgtctatc gcgccggctg tagcgcctgc gagacgtcgg tggcggagcc
     5161 ggcggactgg gtcatgccca ccgacgccat ctggatcgac ctggtcgagc cg
//
LOCUS       DASVVN010000645         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_471905,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000645 DASVVN010000000
VERSION     DASVVN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471905"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..959)
                     /locus_tag="VF686_10585"
     CDS             complement(60..959)
                     /locus_tag="VF686_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8662300.1"
                     /translation="MLFRSLAAAAALLAVSTPVAAQDLDLRAQVAAFVQPTNAARTDV
                     VVAQLRAAGFDPVVETFVGGTERSGAMEGRNVVVTIGDGAREILLTAHYDAVTLRDGT
                     MSHGVVDNAASVVGVIEAAKILRTKALNHRVRIILFDQEELGLIGAKKWIEAHGLTNV
                     AAVVNSDVAGYGDTMMYGQNNGLQSAFVTRAVQTVCSELALQCVGYPAYPPSDDRAFS
                     AAGAPVVSLGFQDQVGAHQMWLAFNGGQTNGLAEGFVPQVFRLIHSNEDTIERVDPAT
                     VRLAGDTYAAVVERLDADLAAVD"
     gene            1093..1302
                     /locus_tag="VF686_10590"
     CDS             1093..1302
                     /locus_tag="VF686_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662301.1"
                     /translation="MSQHPDPQHQPVKGPDDLQRNPGIGQSAGLNRREGLDEIEGDST
                     VEGDVPNNAGPGGAVDPDDRGRENR"
     gene            complement(1299..>1610)
                     /locus_tag="VF686_10595"
     CDS             complement(1299..>1610)
                     /locus_tag="VF686_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8662302.1"
                     /translation="GMLGVFAGLWAQKFDQLAAVQNFVIMPMTFLSGTFYLVENLPEP
                     FATISHFNPFFYLIDGFRAGFIGNAEGNLLIGVVMSGLLTVVMAWACWAVFRSGWRLK
                     S"
BASE COUNT          282 a          537 c          561 g          230 t
ORIGIN      
        1 gccggagagc gtcgggaccg cttgaaaaag ggtcaggcct tgtcggacgg ggggccgggt
       61 tagtcaacag cggccagatc ggcgtccagc cgttcgacca ccgccgcata ggtgtcgcct
      121 gccagccgga ccgtggccgg atcgacgcgc tcgatggtgt cctcgttgga atggatcagg
      181 cggaacactt gcgggacgaa gccttcggcc aggccgttgg tttggccgcc gttgaaggcc
      241 agccacatct ggtgggcgcc gacctggtcc tggaagccca gcgacaccac cggcgcgccg
      301 gcggccgaga aggcgcggtc gtcggatggc ggatacgcgg gatagccgac gcattgcaac
      361 gccagttccg aacagaccgt ctggacggcg cgggtgacga aggccgactg caggccgttg
      421 ttctggccgt acatcatcgt gtcgccgtag ccggcgacgt ccgagttcac gacggcggcg
      481 acattcgtca gcccgtgcgc ctcgatccat ttcttcgcgc cgatcagccc cagttcctcc
      541 tgatcgaaca ggatgatgcg gacgcggtgg ttcagggcct tggtccgcag gatcttcgcc
      601 gcctcgatga cgccgaccac cgaggccgca ttgtcgacaa cgccgtgcga catcgtcccg
      661 tcgcgcagcg tcaccgcgtc atagtgggcg gtcagcagga tttcgcgcgc gccgtcgccg
      721 atggtgacga cgacgttgcg accttccatc gcgccgctcc gttccgtgcc gccgacgaag
      781 gtctcgacga cgggatcgaa gccggcggcg cgcagctggg cgaccacgac atcggtgcgg
      841 gcggcgttgg tcggctggac gaaggcggcg acctgggccc gcaggtccag atcctgggcg
      901 gcgacgggcg ttgagacggc gagaagggcc gcggcggcgg cgagcgaacg gaacagcatg
      961 gaaccccctt gagacgaggc gccatggcta gcgcggcccg cagcgtcgcg caccttaaaa
     1021 ggcggtcacc tgcccccgcg ccggaaccgg tcgcggccgc tttcggttga tttcgtacga
     1081 aaggagtccg ccatgtccca gcatcccgac ccgcagcatc agccggtcaa gggtcccgac
     1141 gatctgcagc gcaaccccgg catcggccag tctgcggggc tgaaccgccg cgagggtctc
     1201 gacgagatcg agggcgacag cacggtcgag ggcgacgtcc ccaacaacgc gggccccggc
     1261 ggcgccgtgg atcccgacga ccgcggccgc gagaaccgct agctcttcag ccgccagccc
     1321 gagcggaaca ccgcccagca ggcccaggcc atgaccacag tcagcaggcc gctcatgacc
     1381 acgccgatca gcaggttccc ctcggcgttc ccgatgaagc cggcccggaa gccgtcgatc
     1441 aggtagaaga acgggttgaa gtgactgatg gtcgcgaacg gctcgggcag gttctcgacg
     1501 agatagaagg tgccgctgag gaaggtcatc ggcatgatga cgaagttctg cacggcggcc
     1561 agctggtcga acttctgcgc ccacaggccg gcgaagacgc ccagcatccc
//
LOCUS       DASVVN010000646         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_315181,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000646 DASVVN010000000
VERSION     DASVVN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315181"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VF686_10600"
     CDS             <1..329
                     /locus_tag="VF686_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8662303.1"
                     /translation="RSTRQRSHGCVRVQGAVEFARLLLSPDPVLLAEFDTAQDTRETK
                     RIQTGREISVRLLYWTAFVDGQGRVAVREDVSSRDATLAEALGIALSLPKPVDDGARV
                     ANDVGP"
     gene            complement(333..848)
                     /locus_tag="VF686_10605"
     CDS             complement(333..848)
                     /locus_tag="VF686_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX8662304.1"
                     /translation="MPTDADIPPSAGRSRAAVVDDFARSELFDRTFREGMELVEDTAA
                     YLDGDGRRESKLLSRATALAYAGESMKLTTRLMQIASWLLVQRAVREGDMTALAACEA
                     RYRLTDRKPETEPTNSELPIALVEYLVRSEKLYDRVLHLDRRMYLDAPEADAVNPVQS
                     QLDRLTAAFRN"
     gene            complement(950..1153)
                     /locus_tag="VF686_10610"
     CDS             complement(950..1153)
                     /locus_tag="VF686_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX8662305.1"
                     /translation="MFEDLEPRPARGAPLIALTREDLDGYSVEDLKQRIAGLEAEIAR
                     SKAAIEGKSSQRNAADAMFNFRP"
     gene            1237..2208
                     /locus_tag="VF686_10615"
     CDS             1237..2208
                     /locus_tag="VF686_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX8662306.1"
                     /translation="MRVIEIAGGAGPAEALTLAERPDPVAGPGQIRIRVRAAGINRPD
                     ILQRLGRYPAPPGASDILGLEVAGEVDQIGDGAGRWKPGDAVCALLGGGGYADYAVVD
                     ARHALPVPAGVNLIQAAALPETVCTVFANVFEAGGLKRGETLLIHGATSGIGVTAIPM
                     ARAAGARVIATSRGPDKAAAALRLGADLSLDALADDMETAIAAFGGADVVLDMVGADY
                     AALNQAALNPFGRWVVIATLSGPAAQIDLARLMMKRIVLTGSTLRARPADEKARLIAA
                     VEATAWPWVASGAVRPPVEATFPLDQASAAHLALEAGDHVGKIVLTT"
BASE COUNT          342 a          802 c          782 g          347 t
ORIGIN      
        1 accggtccac gcgccagcgc agccacggct gtgtccgggt gcagggcgcg gtggagttcg
       61 cccgcctgct gctgtcgccc gatccggtcc tgctggccga gttcgacacc gcccaggaca
      121 cgcgcgagac caagcgcatc cagacgggcc gcgagatcag cgtgcgcctg ctgtactgga
      181 ccgccttcgt cgacggccag ggccgcgtcg ccgtccgcga ggacgtctcc agccgcgacg
      241 ccacactggc cgaagcgctc ggcatcgccc tcagcctgcc caagcctgtc gatgacggcg
      301 cgcgggtggc gaacgacgtc ggaccgtaga atctagttcc ggaacgccgc cgtcagccgg
      361 tccagctggc tctgaaccgg attgaccgcg tccgcctctg gcgcgtccag atacatgcgg
      421 cggtccaggt gcaggacccg atcgtacagc ttctcgctcc ggaccaggta ttcgaccagg
      481 gcgatcggca gttcgctatt cgtgggttcg gtctcgggct tgcggtcagt cagccgatag
      541 cgcgcctcgc acgccgctag cgccgtcatg tccccctcgc gcaccgcccg ctgcaccagc
      601 agccacgagg cgatctgcat cagacgggtg gtcagcttca tgctctcgcc agcgtaggcc
      661 agggccgtgg cccgcgacag cagcttggat tcgcggcggc cgtccccgtc cagataggcc
      721 gctgtgtcct cgaccagttc catgccttcc cggaaggtgc ggtcgaacag ctccgagcgc
      781 gcgaagtcgt cgacgacagc agcgcgactg cggccggcgg acggaggaat gtcggcgtcg
      841 gtgggcatga agtgggtcga actcataagg gaacgcgcat ccgtatcacg accggcacgg
      901 tttcgcgcgg cgcatcaacg gactgcaacg gttgtgccaa caggttgatt cagggccgga
      961 agttgaacat ggcgtcggcg gcgttgcgtt gagacgactt gccctcgatg gcggccttgg
     1021 accgggcgat ctcggcctcc agccctgcga tacgctgctt caaatcctcg accgaatagc
     1081 cgtccagatc ctcgcgtgtc agggcgatca gcggcgcgcc gcgcgccgga cggggctcca
     1141 gatcctcgaa catcgccgcc tccttcgtgg ccaagggata cgcaaccccg tatcaaaacg
     1201 cccggagacg gacgattgca agaacgggat gagtggatgc gggtcattga gattgcgggc
     1261 ggcgcaggtc ccgccgaggc cctgaccctc gccgaacggc cggatccggt ggcgggaccg
     1321 ggtcaaatcc ggatccgggt gcgtgccgcc gggatcaacc ggcccgacat cctgcagcgc
     1381 cttggccgct atccggcccc gccgggcgcg tcggatattc tcggtctgga agtggccggt
     1441 gaagtcgatc agatcggcga cggcgccggc cgctggaagc cgggggacgc ggtctgcgcc
     1501 cttctgggtg ggggcggcta tgccgactac gccgtcgtgg acgcccgaca cgccctgccc
     1561 gtcccggccg gcgtcaatct gatccaggcc gccgccctgc cggaaaccgt gtgcacggtc
     1621 ttcgccaatg tcttcgaggc cggcggcctg aagcgcggtg aaaccctgct gatccacggc
     1681 gccaccagcg gcatcggcgt caccgccatc ccgatggcca gggcggccgg cgcgcgggtc
     1741 atcgccacct cccgcggccc tgacaaggcc gccgccgcgc tgcggctggg cgcggacctc
     1801 agtctggacg ccctggccga cgatatggag acggccatcg cagccttcgg cggcgccgat
     1861 gtcgtgctgg acatggtcgg cgccgactac gccgccctca accaggcggc gctgaacccg
     1921 ttcggccgct gggtggtgat cgccaccctg tcgggcccgg cggcccagat cgatctggcg
     1981 cgactgatga tgaagcggat cgtcctcacc ggctcgaccc ttcgcgcccg ccccgccgac
     2041 gagaaggccc ggctgatcgc ggccgtggaa gccacggcct ggccatgggt cgcctccggc
     2101 gcggtgcggc cgcccgtcga ggcgaccttc ccgctggatc aggcgtcagc cgcccacctc
     2161 gcgctggagg cgggcgacca cgtcggcaag atcgtgctga cgacctagcg gcgtccgcgc
     2221 cggatgggcc gcattgagga aatccggtcg ttgagacccg agttgcgcag gtt
//
LOCUS       DASVVN010000647         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_208399,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000647 DASVVN010000000
VERSION     DASVVN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208399"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1061
                     /locus_tag="VF686_10620"
     CDS             159..1061
                     /locus_tag="VF686_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8662307.1"
                     /translation="MTIDLTRRVAIVTGAGGGLGRSHALALARYGAKVVVNDMSPAGA
                     EAVAGEIQEAGGEAMAWTCSVTDRDAVEAMVAGAVERWGSIDILVNNAGILRDRTFAK
                     MDLDDFAAVIDVHLMGTVNVTKAVWPHMRDKGYGRIIFTTSSSGLYGNFGQSNYSAAK
                     MALVGLMQTLALEGEKSGIRVNCLAPSASTQMTAGLYSEEDLKGLSTDLVSPGVVALA
                     AEGAPTRVVLLAGAGAFEQAHITMTRGIHVGDAPDAAEQIQAQWARISDRSDEAVPPS
                     GAAQYLHEVQHPGRSAPQLQAVGS"
     gene            1066..1833
                     /locus_tag="VF686_10625"
     CDS             1066..1833
                     /locus_tag="VF686_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8662308.1"
                     /translation="MTLSPPLPSFRLDGKRALVTGAGRGIGAAAAAVFAQAGAAVTLC
                     ARTGAELDARVEEIRDAGFEADAFVLDVNDIAAVRREIEARGPFDILLNNAGVNRIRS
                     LVEVTEEDYDAVLNVNLKAALFVAQAVAKGLLEAKKPGSIINVSSQMGHVGGAGRSLY
                     SASKWGLEGLTRCMAIDLAPHGIRVNTICPTFIDTALTRPILDTPGTREHVLSRIKLG
                     RLGRMDELTGPLLLLASDASTLMTGSSLILDGGWTAG"
     gene            complement(1839..>2127)
                     /locus_tag="VF686_10630"
     CDS             complement(1839..>2127)
                     /locus_tag="VF686_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662309.1"
                     /translation="GSTGWNFGAGMSGGEAFIHDPDGTALVRVNGDSVASGVVAGEAA
                     ARLRALVARHAAETRSPLALRLIEMWPTAVGQFRQLLPRTEVQTSVSARRA"
BASE COUNT          355 a          715 c          721 g          336 t
ORIGIN      
        1 cccggcgctc ttgcgacgcc atgggagaga cggtcccact gaaccgaccc cgtgtcaatc
       61 ttcactagcc gacgagtgaa tattgacctt ctggccaatg tcagggcagg ttccgcatcc
      121 ccgttcgcag agggcggcga gcgattgcgg agcgacccat gaccattgac ctgacccgcc
      181 gcgtggcgat cgtgaccggc gccggaggcg gcctcggccg cagccacgca ctggccttgg
      241 cgcgttatgg agccaaggtc gtcgtcaacg acatgtcgcc ggccggggct gaagcggtgg
      301 cgggggaaat ccaagaagcg ggcggcgagg ccatggcctg gacgtgcagc gtcaccgatc
      361 gcgacgccgt ggaggccatg gtcgccgggg cggtggagcg ctggggatcg atcgacattc
      421 tggtcaacaa cgcgggcatc ctgcgggacc gcaccttcgc aaagatggat ctcgacgact
      481 tcgcggccgt gatcgacgtg catctgatgg gaacggtgaa cgtgaccaag gccgtctggc
      541 cgcacatgcg cgacaagggc tacggccgca tcattttcac gacgtcctcg tcgggcctct
      601 acggcaattt cggccagtcc aactattcag ccgccaagat ggctctggtg ggcctgatgc
      661 agacccttgc gctggagggc gagaagtcag gcatccgcgt caactgtctg gcgcccagcg
      721 cctccacgca gatgacggcc ggtctctact ccgaagagga tctgaaaggt ctgtccacgg
      781 atctggtcag ccccggcgtt gtcgcactgg cggccgaggg ggccccgacg cgggtcgttc
      841 tgctggcggg cgcgggcgcc ttcgaacagg cccacatcac gatgacccgg ggaatccatg
      901 tcggcgacgc gcccgacgcg gccgaacaga tccaggccca atgggcgagg atttcggacc
      961 gctcggatga agccgtcccc ccttcgggcg cggcccagta ccttcacgag gtccagcatc
     1021 cgggccgaag cgcgccacag cttcaggccg tcggcagtta ggaccatgac cctcagcccg
     1081 cccctgccct cgtttcggct cgacggaaag agagcgctcg tcacgggggc ggggcgcggc
     1141 atcggcgcgg ccgccgccgc ggtcttcgcc caggccgggg ccgccgtcac cctgtgcgcg
     1201 cgaaccggcg ccgagctgga cgcccgggtc gaggaaatcc gggacgcggg gttcgaggcg
     1261 gacgcgttcg ttctggatgt caacgacatc gccgccgtcc ggcgagagat agaagcgcga
     1321 ggcccctttg acatcctgct caacaacgcc ggggtgaatc gcatccggtc tctcgtagag
     1381 gtgacggagg aggactatga cgccgtcctg aacgtcaatc tcaaggccgc gctgtttgtc
     1441 gcgcaggcgg tggcgaaagg gctcctggag gcgaagaagc ccggctcgat catcaatgtt
     1501 tcgtcgcaga tgggccacgt gggcggggct ggccgatcgc tttacagcgc ttcgaaatgg
     1561 ggactggagg gcttgacccg ctgcatggcg atcgacctcg cgccccacgg cattcgcgtg
     1621 aacaccatct gtcccacctt catcgacacg gccctgacac gcccgatcct cgacactccc
     1681 ggcacgcgcg agcatgtgct cagccggatc aagctcggcc ggcttggccg catggacgag
     1741 ctgaccggcc cgcttcttct gctggcgtct gacgcctcaa ccctgatgac cggaagctcg
     1801 ctgatcctgg acggcggctg gacggcgggc tgaggcgatc acgcccgtcg tgcgctgacc
     1861 gatgtctgta cttctgtcct cggcaggagt tggcgaaact ggccgacggc ggtcggccac
     1921 atctcgatca ggcggagtgc gagcggggac cgggtttccg ccgcatggcg tgcgaccagc
     1981 gcccgaagcc gcgccgccgc ctcgcccgca acgacgccgc tcgcgaccga gtcgccgttg
     2041 acgcgcacga gggcggtccc gtcaggatcg tggatgaacg cctcgccgcc cgacatcccg
     2101 gccccgaaat tccaccctgt cgagccg
//
LOCUS       DASVVN010000648         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_25301, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000648 DASVVN010000000
VERSION     DASVVN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25301"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF686_10635"
     CDS             complement(<1..326)
                     /locus_tag="VF686_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8662310.1"
                     /translation="MAAEFDLIVIGSGPGGYVAAIRASQLGQKVAIVERESLGGICLN
                     WGCIPTKALLKSAEKYESLSHLKDYGLSADKVGFDFDTIIQRSRGVAATMNKGVTYLM
                     KKNKIEV"
     gene            complement(328..807)
                     /locus_tag="VF686_10640"
     CDS             complement(328..807)
                     /locus_tag="VF686_10640"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX8662311.1"
                     /translation="MASVYDFAAKGIDGTAVALEQYRGQALLIVNTASKCGFTGQYAG
                     LEALHRDYADRPFEVLGFPCNQFGQQEPGKAAEIASFCSSTYGVDFPMFDKVEVNGPN
                     RHPLFDWLTSQKKGFLGSREIKWNFTKFLTDREGRVVARYAPQVEPEAIKADIEKLI"
     gene            complement(957..2255)
                     /locus_tag="VF686_10645"
     CDS             complement(957..2255)
                     /locus_tag="VF686_10645"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX8662312.1"
                     /translation="MTDILMPALSPTMEEGVLAKWHVKVGDTVKAGDVIAEIETDKAT
                     MEVEAVDEGEVTDILVAEGTEGVKVNTPIARLKDEGGAAAPAPKTAAAPAAAKAEPAP
                     AGHESGQAVATPQGVKAAEAILPKSSSQTSGERVFASPLARRIAAQNGVDLKSVKGTG
                     PHGRIVKRDVEGVKGGAPAAASASAPAAEARKVQSLAQMGIPDGSYDLIPLDGMRKAI
                     ARRLTDSFRDVPHFPLTIDCEIDGLLASRAKVNAMLEKSGVKVSVNDFVIKASAMALK
                     AVPEANASYSPEGIAMHHNADVAMAVAIDGGLITPIIRKAETKSLSQIATESKDLAKR
                     ARDRKLKPEEFQGGTFSVSNLGMFGIKAFASIINEPQGAIMSVGAGEQRPVVKNGQLA
                     VATVMTVTLTCDHRVVDGAVGARFLQAFKAMIEDPVTMLA"
     gene            complement(2252..2569)
                     /locus_tag="VF686_10650"
     CDS             complement(2252..2569)
                     /locus_tag="VF686_10650"
                     /inference="COORDINATES: protein motif:HMM:NF028136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5076 domain-containing protein"
                     /protein_id="HEX8662313.1"
                     /translation="MSANPEFQLTTPDSVAADPEAVELLRMWWSKGEPVMAVKPAFND
                     PIQFGQMLAYAAKHMAHGYAVRHGHDERQAYQRILQGLSDVIKADNVQTVAEPVAQPG
                     SVQ"
     gene            complement(2566..>2944)
                     /locus_tag="VF686_10655"
     CDS             complement(2566..>2944)
                     /locus_tag="VF686_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8662314.1"
                     /translation="VGSDVTLVAYSRMVGFCLQAAEKLAAEGIEAEVIDLRTIRPLDH
                     QTIVESVKKTNRIVCVEEGWGPMGVGAEIVARVVEHAFDYLDAPPLRVHQEDVPLPYA
                     ANLEALSLPGVDKIVAAVKQVMA"
BASE COUNT          437 a          979 c          977 g          551 t
ORIGIN      
        1 acctcgatct tgttcttctt catcagatag gtgacgccct tgttcatcgt ggcggccacc
       61 ccgcgcgagc gctggatgat ggtgtcgaag tcgaaaccga ccttgtcggc ggacagcccg
      121 tagtccttca ggtgcgacag gctctcgtat ttctcggccg acttgagcag ggccttggtc
      181 gggatgcagc cccagttcag gcagatgccg cccaggcttt cgcgctcgac gatggcgacc
      241 ttctggccca gctggctggc gcggatggcg gcgacgtatc cgccggggcc cgaaccgatg
      301 acgatgaggt cgaattcagc agccatgtca gatcagcttc tcgatgtcgg ccttgatggc
      361 ctcgggttcg acctgggggg cgtagcgcgc gaccacccgc ccctcccggt ccgtcaggaa
      421 tttggtgaag ttccacttga tctcgcggct gcccaggaaa cccttcttct ggctggtcag
      481 ccagtcgaac agagggtgcc ggttggggcc gttgacctcg accttgtcga acatcggaaa
      541 gtcgacgcca taggtcgagg agcagaagct ggcgatctcg gcggccttgc cgggctcctg
      601 ctggccgaac tggttgcagg gaaagcccag cacctcgaac ggcctgtcgg catagtcccg
      661 atgcagcgcc tccagccccg cgtactggcc ggtgaagccg catttggacg cggtgttgac
      721 gatcagcagg gcctggccgc gatactgctc cagcgcgacc gcggtcccgt cgatgccctt
      781 cgccgcgaaa tcatagaccg aggccaatct aaactccgtc accctcgggc ttgtcccgag
      841 ggcccatggt tccggcgcca cgctcggcgc cgcttgggtc gtgccgtcaa gaccttcgac
      901 cgcgcctgcg gatcgatggg tcctcgggac acgcccgagg atgacggcgg tgtgggttac
      961 gccagcatcg ttacaggatc ttcgatcatc gccttgaagg cctgaaggaa tctcgcgccg
     1021 acggcgccgt ccaccacgcg gtgatcgcag gtcagggtga cggtcatcac cgtggccacg
     1081 gccagctggc cgttcttgac cacgggacgc tgctcgcctg cgcccacgct catgatcgcg
     1141 ccctgcggct cgttgatgat cgaggcgaag gccttgatgc cgaacatgcc gaggttggag
     1201 accgagaagg tgccgccctg gaactcctca ggcttcagct tgcggtcgcg ggcgcgcttg
     1261 gccaggtcct tggactcggt cgcgatctgc gacagggact tggtctcggc cttgcggatg
     1321 atcggggtga tcaggccgcc gtcgatcgcc accgccatcg ccacgtcggc gttgtggtgc
     1381 atggcgatgc cctcgggcga atagctggcg ttggcttccg gaaccgcctt cagcgccatg
     1441 gccgaggcct tgatgacgaa gtcgttgacc gagaccttca cgcccgactt ctcgagcatg
     1501 gcgttgacct tggcgcgcga ggccaacaag ccgtcgatct cgcaatcgat ggtcagcggg
     1561 aaatgcggca cgtcgcggaa gctgtcggtc aggcgccgcg cgatcgcctt gcgcatgccg
     1621 tccagcggga tcaggtcgta gctgccgtcc gggatgccca tctgggccag cgactggacc
     1681 ttgcgggcct cagcggcggg cgccgaagca gatgcagcgg ccggagctcc gcccttcacg
     1741 ccctcgacgt cgcgtttgac gatccggccg tgcgggccgg tccccttgac ggatttcagg
     1801 tcgacgccgt tctgcgcggc gatgcgccga gccagcggcg aggcgaagac gcgctctccg
     1861 gacgtctgcg acgaagattt cggcaggatg gcctcagccg ccttcacgcc ttggggcgtg
     1921 gccacggcct gcccgctttc atggcccgca ggtgcgggct cggccttggc cgcggcggga
     1981 gccgcagccg tcttcggcgc cggagcggcc gcgccgccct catccttgag acgggcgatc
     2041 ggcgtattga ccttcacgcc ttccgtgcct tcagccacga ggatgtcggt gacctcgcct
     2101 tcgtcgacgg cctcgacctc catggtcgcc ttgtcggtct cgatctcggc gatgacgtcg
     2161 ccggccttga ccgtgtcgcc gaccttgacg tgccatttgg ccagcacgcc ctcttccatc
     2221 gtgggcgaca gtgcgggcat caggatgtcg gtcattgcac gctcccgggc tgggcgaccg
     2281 gctcggccac ggtctgcaca ttgtccgcct tgatcacgtc ggacagccct tgaaggatcc
     2341 gctgataggc ctgccgctcg tcatggccgt gccgcaccgc atagccgtgg gccatgtgtt
     2401 tggcggcgta ggccagcatc tgaccgaact ggatggggtc gttgaaggcc ggcttgaccg
     2461 ccatgaccgg ctcgcccttc gaccaccaca tgcgcagcag ttctacggcc tcgggatcgg
     2521 cggcgacgct gtccggcgtc gtcaactgga actcgggatt tgcgctcatg ccatcacctg
     2581 cttcacggcg gcgacgatct tgtccacgcc cggcagcgac agggcttcca gatttgcggc
     2641 gtagggcagc gggacgtctt cctggtggac gcgcagcggc ggggcgtcga ggtagtcgaa
     2701 ggcgtgctcg acaacccggg cgacgatctc ggcgccgacg cccatcgggc cccagccttc
     2761 ctcgacgcag acgatgcggt ttgtcttctt gacgctctcg acgatggttt gatggtcgag
     2821 ggggcggatg gtgcgcaggt cgatgacctc cgcctcaatc ccctcggccg ccagcttctc
     2881 ggccgcctgc aggcagaacc cgaccatccg ggaataggcc acgagggtga cgtcgctccc
     2941 cacg
//
LOCUS       DASVVN010000649         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_102735,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000649 DASVVN010000000
VERSION     DASVVN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102735"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF686_10660"
     CDS             <1..854
                     /locus_tag="VF686_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8662315.1"
                     /translation="HLADGCVTGKSEAAIDLEAEYNNRARVPDHPAVMAMWRETAEAA
                     RAAHPPIEIAYGPGEREVMDLFDAGPDAPVAVFLHGGYWQALDRRWFSGLAPAFLAHG
                     VSLAVPSYDLAPAVRLGRILLQVRAAVEMIRARTGVRPVVFGHSAGGHMAACMLSEGR
                     ASAAVAISGVFDLRPLVATSLNTALDLDEREAAALSPVFWPVPDGSTPGGRILDCVVG
                     GEESPEFLRQSRMMADHWAAKGVETRFEALPGLNHFTVLDPLFDADSALVRRIVGLAK
                     AFVPGRR"
     gene            1242..1913
                     /locus_tag="VF686_10665"
     CDS             1242..1913
                     /locus_tag="VF686_10665"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX8662316.1"
                     /translation="MRHDRLAVLTALESQGVAPVFYHPDPQVSLNVIRACARGGAKAI
                     EFTNRGDFACDLFGDIARELQKSDPDIILGIGSVVDAGTASLYLNRGARFVVSPCLVP
                     DVARVCNRRMTAYFPGCGSVTEIGQAHELGCEIVKLFPGASVGGADFVKAVLGPMPWT
                     KIMPTGGVDPDEASIAKWFGSGIVAAGMGSKLVTDAAVKAGDWAGIEANVRKTVDAIA
                     AFRAK"
BASE COUNT          297 a          753 c          638 g          338 t
ORIGIN      
        1 ttcatctagc ggatggttgc gtgacgggta agagcgaagc ggcgatcgac ctcgaggccg
       61 aatataacaa ccgcgcccgc gtgcccgacc atccggccgt tatggccatg tggcgcgaaa
      121 cggccgaagc cgcccgcgcc gcccatcctc cgatcgagat cgcctacggc ccgggcgaac
      181 gcgaggtcat ggacctgttc gacgcgggcc ccgacgcgcc cgtcgccgtc ttcctgcacg
      241 gcggctactg gcaggcgctg gacaggcgct ggttcagcgg cctcgccccc gccttcctcg
      301 cccacggcgt cagcctggcc gtcccctcct acgacctcgc ccccgccgtc cgcctcggcc
      361 gcatcctgct tcaggttcgc gcggcggtag agatgatccg cgcccgcacc ggcgtccggc
      421 cggtcgtgtt cggccactcg gccggcggcc acatggcggc ctgcatgctg tccgagggcc
      481 gcgcctccgc cgccgtcgcc atcagcggcg tcttcgacct gcgtcccctt gtcgccacct
      541 ccctgaacac cgccctcgat ctcgacgaac gcgaggcggc cgccctgtcg ccggtgttct
      601 ggcccgtacc cgacggctcc acccctggcg gcaggatcct cgattgcgtc gtcggcggcg
      661 aggagagtcc cgaattcctg cgccagtcgc gcatgatggc tgaccactgg gccgccaagg
      721 gcgtcgagac ccgcttcgaa gccctgccgg gcctgaatca tttcaccgtg ctggatccgc
      781 tgtttgacgc ggacagcgcc ttggtcagac ggatcgtcgg cctcgccaag gctttcgtcc
      841 ccggccgccg ataaccggat cgcggcgccg gcgccaccgg tgacaatcca tcggctcgcc
      901 gcctttattc atcgcttgac caatgacgcg aatgattgtg tttgcgcgcg tccggcttgc
      961 aatccctccc cacccggttg cgtctttcag agcttcatgt ttcgcgccct tcgaaaattc
     1021 ctgcgccagc tgcgctgccg ccgtcaccgc tggcggcccg accgccgcgt gtcgggacgt
     1081 gagttctgcg atctctgcgg cgccaaacgg caaagggcct gaaccggccc tccgttgcga
     1141 aacacaaagt tccgccgccc tctccgccag cgctttcccg gaacgccgcg ggtgcgataa
     1201 gcatggcggg ctgccggacc gccggcggag gaaatcagac catgcgccac gaccgtctcg
     1261 ccgtcctgac cgcccttgaa tcccagggcg tggccccggt cttctaccat cccgatccgc
     1321 aggtcagcct gaacgtcatc cgcgcctgcg cccgcggcgg ggcgaaagcg atcgagttca
     1381 ccaaccgcgg cgacttcgcc tgcgacctgt tcggcgacat cgcccgcgag ctgcagaaga
     1441 gcgaccccga catcatcctg ggcatcggct cggtggtcga tgcgggcaca gccagccttt
     1501 atctcaaccg cggcgcgcgg ttcgtggtca gcccctgcct cgtccctgat gtcgcccgcg
     1561 tctgcaaccg gcgcatgacg gcctacttcc ccggctgcgg ctccgtcacc gagatcggcc
     1621 aggctcacga gctgggctgc gagatcgtca agctgttccc cggcgccagc gtcggcggcg
     1681 ccgatttcgt caaggcggtc ctcggtccca tgccctggac caagatcatg ccgaccggcg
     1741 gcgtcgatcc cgacgaagcc tcgatcgcca aatggttcgg cagcggcatc gtggccgccg
     1801 gcatgggctc aaaactcgtc accgacgcgg ccgtcaaggc cggcgactgg gccggcatcg
     1861 aggccaatgt gcgcaagacg gtcgacgcga tcgcggcgtt ccgggcgaag tagtggctag
     1921 tggctagtgg ctagtggcta gcgatgtagc ttcgctgacg gcggaaaggc gacgcgatca
     1981 gcgcggccgc ccaggtggtg gccagccact agccactagc cactag
//
LOCUS       DASVVN010000650         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_51309, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000650 DASVVN010000000
VERSION     DASVVN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51309"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VF686_10670"
     CDS             complement(<1..1323)
                     /locus_tag="VF686_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX8662317.1"
                     /translation="MDGNAGGPTPLNDPKKEPAALRTLLGRNNRDWWPNHLTMDILHQ
                     QGRSGDPMGDGFNYAEAFKKLDYAAVKRDLTALMTDSQPWWPADYGHYGPFFIRMAWH
                     SAGTYRTGDGRGGANAGQQRFAPLNSWPDNGNLDKARRLLWPIKQKYGASLSWADLMI
                     LAGNVAIESMGGPVFGFGGGRADVWEPEKDVYWGTEEEWLGETRIDEESGKALESPLA
                     AIQMGLIYVNPEGPAGSADALKSARDMRETFSRMGMNDEETAALTAGGHTFGKAHGAG
                     DASKVGAAPEGSDIAQQGLGWQSGHESGMGDHTITSGLEGAWTPTPTKWDMTFFDMLL
                     DHDYELVRSPAGAKQWQPVGNPDETLAPGAHSPHRKVPTMMTTADMALKVDPGYRAIM
                     EKFRADPDYFADAFARAWFKLCHRDMGPKVRYLGPEVPAEDLIWQDPIP"
     gene            complement(1444..>1832)
                     /locus_tag="VF686_10675"
     CDS             complement(1444..>1832)
                     /locus_tag="VF686_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662318.1"
                     /translation="DADIVARLSSNPNPQQLRDQLAAERRDDPWATRIEAGIRERYGR
                     TPVAARSLDTLSVACAATLCEVIGRTRSGASSDDVAALLGEVQSGELNASIEQLGLEI
                     RSSAFTADPSSPDRMAFVAHLERSGA"
BASE COUNT          266 a          629 c          606 g          331 t
ORIGIN      
        1 cgggatcggg tcctgccaga tcagatcctc ggccggcact tcgggcccga ggtagcggac
       61 cttcggaccc atgtcgcgat ggcacagctt gaaccacgca cgcgcgaagg cgtcggcgaa
      121 atagtccgga tcggcccgga acttctccat gatggcgcgg tagccggggt cgaccttcag
      181 cgccatgtcg gcggtggtca tcatggtcgg gaccttgcgg tgcgggctgt gggcgccggg
      241 cgccagcgtc tcgtccggat tgcccaccgg ttgccactgc ttggccccgg ccgggctgcg
      301 gaccagttcg tagtcgtggt ccagcagcat gtcgaaaaag gtcatgtccc actttgtcgg
      361 cgtcggcgtc caggcgcctt ccagacccga ggtgatggta tggtcgccca tgccgctctc
      421 gtggccggac tgccagccga gaccctgctg ggcgatgtcc gagccctcgg gcgccgcgcc
      481 gaccttggac gcatcaccgg ccccgtgcgc cttgccgaag gtgtggccgc ccgccgtcag
      541 ggccgccgtc tcctcgtcgt tcatccccat gcgcgagaag gtttcgcgca tgtcccgcgc
      601 cgacttcagc gcgtcggcgc tacccgccgg gccctcggga ttgacgtaga tcaaccccat
      661 ctggatcgcc gccagcgggc tttccagcgc cttgcccgac tcctcgtcga tgcgggtctc
      721 gcccagccat tcttcctcgg tgccccagta gacgtccttc tccggctccc aaacgtcggc
      781 ccggccgccg ccgaagccga acaccggtcc gcccatgctc tcgatggcga cattgccggc
      841 caggatcatc agatcggccc acgacaggct ggccccgtat ttctgcttga tcggccacag
      901 caggcgccgc gccttgtcga ggttgccgtt gtccggccag ctgttcagcg gcgcgaaccg
      961 ctgctggccc gcattggcgc cgccgcgccc gtcgccggtg cgataggtcc ccgccgaatg
     1021 ccaggccatg cggatgaaga agggcccgta gtggccatag tccgccggcc accacggctg
     1081 gctgtcggtc atcagggcgg tcaggtcgcg tttcaccgcc gcatagtcga gctttttgaa
     1141 agcctcggcg tagttgaaac cgtcgcccat cggatcgccg gaccggccct gctgatgcag
     1201 gatgtccata gtcagatgat tgggccacca gtcgcggttg ttgcgtccca gcagggtccg
     1261 cagcgccgcg ggctcctttt tcggatcatt caggggagtg gggccgccag cgtttccgtc
     1321 catggtttcc tcctgcgtcg agcgcgattg gaagggaccc tatgcccgcc tctgagggaa
     1381 ggaaaatcga taaactttgt ggtcaggaac aggagaagtt atcgatgagg cggcccggtc
     1441 tcctcaggcg cctgaccttt ccagatgggc gacgaaggcc atccggtcgg gactgctcgg
     1501 gtcggcggtg aaagccgacg agcggatctc caggcccaac tgttcgatcg aggcgttcag
     1561 ttcgccggat tgaacctcgc ccagcagcgc cgctacatcg tcgctgcttg cgccggaccg
     1621 cgtccgtcct atgacctcgc acagggtcgc cgcgcacgcg accgacaacg tgtccaggga
     1681 ccgggccgcc acgggagtcc ggccgtatcg ctcgcgaatg cccgcctcga tccgggtcgc
     1741 ccacgggtcg tcgcggcgct cggcggccaa ctggtcgcga agctgctggg gattggggtt
     1801 ggacgagagg cgggccacga tgtccgcgtc cg
//
LOCUS       DASVVN010000651         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_315290,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000651 DASVVN010000000
VERSION     DASVVN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315290"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF686_10680"
     CDS             <1..442
                     /locus_tag="VF686_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8662319.1"
                     /translation="LLKKERFERVLDVGCGTGVLAIAAAKTGSKVAVGTDIDQPSVRI
                     ANENAKLNQASARFVHAFGLNDRKVRGQGPYDLVFANILAPPLVSLSQDIKEALSLGG
                     VAILSGLLRTQERRVSAAYLSRGFILERRIHRDAWSALVLRRVG"
     gene            497..1000
                     /locus_tag="VF686_10685"
     CDS             497..1000
                     /locus_tag="VF686_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662320.1"
                     /translation="MTHYDPNDPNRIPPVETVYTETVTTAAPLHTETVETTVVREANN
                     TGWWIAGILGGLVIIAVLWILFSRGAEPQTDEALLQAQADAAAAQAQADSALIQGQVA
                     GAQASVDIARADAARSQADAIRAASEARAAEARSSAPVIIEREVQVPAPAPSNGVAVV
                     TPTSPQN"
     gene            1040..>1529
                     /locus_tag="VF686_10690"
     CDS             1040..>1529
                     /locus_tag="VF686_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX8662321.1"
                     /translation="MRQSFDETTDPSFGAKHLPRVRARMAEQGLDGLLVPHEDEHQNE
                     YLPAANDRLAWLSGFTGSAGAGVVMKDRAAVFADGRYTVQVRAQVDADQFEILELENG
                     GVASYLERAPKGSVIGYDPRLHSPDALATLKRAAAKAGAELKPVEPNPLDMAWAEERP
                     GQP"
BASE COUNT          252 a          521 c          535 g          221 t
ORIGIN      
        1 actgctgaag aaggaacggt tcgagcgggt gctggacgtc ggctgcggca ccggcgtgtt
       61 ggccatcgcg gcggcgaaga ccggatcgaa ggtggcggtc ggcaccgaca tcgaccagcc
      121 ctcggtgcgc atcgccaacg agaacgcgaa attgaatcag gccagcgccc gcttcgtcca
      181 cgccttcggt ctgaacgatc gcaaggtgcg ggggcagggg ccgtacgatc tggtgttcgc
      241 caacatcctg gcgccgccgc tggtgagcct gtcgcaggac atcaaggagg cgctgagcct
      301 cggcggggtg gccatcctct cgggcctgct gcggacgcag gaacggcgtg tgtcggcggc
      361 ctatctgtcc cggggattca tcctcgaacg gcgcatccat cgcgacgcat ggagcgcgct
      421 ggttctgagg cgggtgggtt aacggcggaa cgtcggcgaa gcgcagccgt tgcggcagtc
      481 cattggagga ctgcccgtga cccactacga cccgaacgac ccgaaccgca taccgcccgt
      541 cgagacggtc tataccgaga cggtgacgac ggccgcgccg cttcataccg aaaccgtcga
      601 gaccacggtg gtgcgcgaag cgaacaacac cggctggtgg atcgccggca ttctcggcgg
      661 tctggtgatc atcgccgtgc tgtggatcct gttctcgcgc ggcgccgagc cgcagactga
      721 cgaggccctg ctgcaggccc aggccgatgc agcggccgcc caggcgcagg ccgactcagc
      781 cctcatccaa gggcaggtcg ccggagcgca ggccagcgtc gacatcgccc gcgccgacgc
      841 cgcccgctcg caggctgacg cgatccgcgc cgccagcgag gcgcgtgccg ccgaagcacg
      901 ttcttcggcc cctgtgatca tcgagcggga ggttcaggtt cccgcccccg cgccctcgaa
      961 cggcgtcgcc gtggtgactc cgacttcgcc gcaaaactga cggcttgcga cgtggcggcg
     1021 ggacgctagt ttcgccgcca tgcgccagtc cttcgacgaa accaccgatc cgtccttcgg
     1081 ggcgaaacac cttccccgcg tccgcgccag gatggccgag cagggcctcg acgggctgct
     1141 ggtcccgcac gaggacgagc accagaacga atatctgccc gccgccaacg accggctggc
     1201 ctggctgagc ggcttcaccg gttcagccgg ggctggcgtg gtcatgaagg accgcgccgc
     1261 tgtcttcgcc gacggccgct acaccgtgca ggtccgggcc caggtcgatg cggaccagtt
     1321 cgagatcctc gaactcgaga acggcggcgt cgccagctat ctggagcgcg cgcccaaggg
     1381 gtcggtgatc ggttacgacc cgcgtctgca cagtcccgat gcgctggcga cgctgaagcg
     1441 cgcggcggcg aaggcggggg ccgagctgaa gccggtcgag cccaatccgc tggatatggc
     1501 gtgggccgaa gagcgtcccg gacagccga
//
LOCUS       DASVVN010000652         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_260704,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000652 DASVVN010000000
VERSION     DASVVN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260704"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF686_10695"
     CDS             <1..442
                     /locus_tag="VF686_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8662322.1"
                     /translation="VGRATLYRNFPGRTEIALAVLLDDVAELGERFAEPTSPDAFLDF
                     IAGLSDRLVRNTALGGVVRAAPSPEILKPLRLAMVEAAMPALKTSQAAGVVRADLAAS
                     DIRVLSAMLGAGLHNAAPRERNMIAARSLEFVLDAVRAVPGRQA"
     gene            439..>1737
                     /locus_tag="VF686_10700"
     CDS             439..>1737
                     /locus_tag="VF686_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8662323.1"
                     /translation="MSRRNFHLPWAQYVETLQPHERPMLPGSPATPDHTIAYRLAYAA
                     IGLLVTATGSIGSAMITANTQQLAGALGVSIVEAAWLPIVFVMTNACMNLLLIKFRQQ
                     YGLRLFAQIFLIAYVAIGVLGLIFHDYQSTLIVRGFAGMASAAMSSLGILYLIQAFPP
                     AHRLKGLILGIGASSLAIPAVRLFTTQLVDISDWQGFHVFELGLSLMSLAAVFALRLP
                     PSEKLKVFDKLDFVTFALFAPGMALLCAVLGLGRIVWWTEAAWVGWALIGTIVLLGAA
                     LIIEFNRDTPLINLRWMAGPDIVRLGLAIVGVRIVLSEQTAGAVGLMTQLGVGPDQMH
                     GLFALILIATAAGSLTSAFTLNMEKLNKPIAIALGLIAVGAFIDSHATTLTRPVQLYF
                     SQALLAFAAAFFIGPAMMQGVVQVLQKGAQNLVSFIVVFGI"
BASE COUNT          248 a          604 c          549 g          336 t
ORIGIN      
        1 cgtaggacgg gctacgctct atcggaactt tcccggacga accgagattg ctctcgccgt
       61 gttgctggat gatgtcgccg aactcggcga gcgcttcgca gagccgacat cgcccgacgc
      121 ctttctggac tttatcgcag gcctgtccga ccgtctggtc agaaacacgg ccttgggagg
      181 cgtcgtccgg gcggcgccgt cgcccgaaat cctgaaaccc ctgcgcctcg ccatggtcga
      241 ggcggccatg cccgccctca agacgtcaca ggccgccggc gtggtgcgcg ccgacctcgc
      301 ggcgtcggac atccgggtgc tttcggccat gctcggcgcg ggcctgcaca acgccgcgcc
      361 gcgcgagcgg aacatgatcg cggcccgctc gcttgagttc gtgctggacg ccgtgcgggc
      421 ggtccccgga cgccaggcat gagccgtcgc aacttccacc tgccctgggc ccagtacgtc
      481 gagaccctgc agccgcacga acggccgatg ctgcccggct cgcccgccac gccggatcac
      541 accatcgcct accggctggc atacgccgcg atcggcctgt tggtgaccgc caccggttcg
      601 atcggctcgg ccatgatcac ggccaacact cagcaactgg cgggcgcctt gggggtctcg
      661 atcgtcgagg cggcgtggct gccgatcgtg ttcgtgatga ccaacgcctg catgaacctg
      721 ttgctgatca aattccgcca gcagtacggg ctcaggctgt tcgctcagat tttcctgatc
      781 gcctacgtcg ccatcggcgt gctgggcctg atcttccacg actaccagtc aacgctgatc
      841 gtgcgcggtt tcgccggaat ggcgtcggcg gccatgtcct cgttgggcat cctctacctg
      901 atccaggcct tcccaccggc gcatcgccta aagggtctta tcctcggcat cggcgcctcc
      961 agcctcgcca tcccggcggt gcggctgttc accacccagc tggtggatat cagcgactgg
     1021 cagggcttcc acgtctttga actggggctg tcgctgatgt cgctggcggc cgtgttcgcc
     1081 ctgcgcctcc ctccgtcgga gaagctgaag gtcttcgaca agctggattt cgtgaccttc
     1141 gcgctgttcg cccccggaat ggccctgttg tgcgcggtcc tcggcctcgg ccggatcgtc
     1201 tggtggaccg aggccgcgtg ggtcggctgg gccctgatcg gcaccatcgt cctgctgggc
     1261 gccgcactga tcatcgagtt caatcgggac acgccgctga taaacctccg atggatggcc
     1321 ggaccggaca tcgttcggct gggcctcgcc atcgtcgggg tgcggatcgt cctgtcggag
     1381 cagacggccg gcgcggtcgg cctgatgacc cagttgggcg ttggacccga ccagatgcat
     1441 ggcctgttcg cgcttatcct gatcgcgacg gcggcgggca gtctgaccag cgccttcacg
     1501 ctgaacatgg agaagctgaa caagccgatc gccatcgccc tgggcctcat cgccgtcggc
     1561 gcctttatcg acagccatgc gacgacgctg acccgccccg tccagctcta tttcagccag
     1621 gccctgctgg ccttcgccgc cgccttcttc atcggcccgg cgatgatgca gggcgtggtt
     1681 caggtgctgc agaagggcgc gcagaatctg gtcagcttca tcgtcgtctt cggcatc
//
LOCUS       DASVVN010000653         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_236458,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000653 DASVVN010000000
VERSION     DASVVN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236458"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VF686_10705"
     CDS             complement(<1..844)
                     /locus_tag="VF686_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662324.1"
                     /translation="MRIVVCALAALSLAACVSVPQVPNARPLNASHIEALGPTPVTVS
                     ENNTRVGKAWMAQDSTAAGATQGMIGVLFSVAFDAMMNAGPSRRAAQAADEVAEVMPV
                     DALNASLVEHFRRQAAAEPASGVTVSDVKLVQRRTAPIVINDNVEVGTSYLLSEDATT
                     LQVVVNLSYTNPAVPYVTPYTFEGAPPKSELTGPLYRNSFTYYSRQLPAPVLTPELRE
                     RLIASIQDSARDAAGALPAEGTDAFKAMNRELEQARDDRLTKAEIAIFLTREWSKDNG
                     ALLRA"
     gene            complement(957..2444)
                     /locus_tag="VF686_10710"
     CDS             complement(957..2444)
                     /locus_tag="VF686_10710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX8662325.1"
                     /translation="MNAFAPLAFPGPADSGSIPSMPHNLEAEQALLGSLMFDNAVFER
                     LSDRLRGSHFYEPFHQRLYEAIEDHIRQGMLAEPTILMERFKQDPAFQEFGGLRYLAD
                     LVDRAPPAANAPDYARVVYDLALRRDLIRIGGEIVREAPQPETAAIDQIEQAEQRLYT
                     LAETGKPSSGFVSFSSALSGAVQMAAEAYQRDGKLAGLATHLDDLDAKLGGLHPSDLL
                     VLAGRPSMGKTALATNIAFNVARNYRWEPTPEGRKTVNGGVVAFYSLEMSAEQLAMRI
                     LADASGVSSDKLRKGEIDAGDFGKIREAAVEIGESPLFIDATGGLSISKLAARARRLK
                     RMEHGLDLIIVDYLQLITVGDGNNQKNRVQEVSEITGALKALAKELSVPIIALSQLSR
                     QVEQREDKRPQLSDLRESGSIEQDADCVMFVYRESYYLGRAEPREGSPEHLQWQEDMD
                     RLEHQAEVVIGKQRHGPIGIIKLSFDANTTKFGNLAQGQRYDNYD"
     gene            complement(2578..2952)
                     /locus_tag="VF686_10715"
     CDS             complement(2578..2952)
                     /locus_tag="VF686_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662326.1"
                     /translation="MTRIILIAGLALAMAGSAFAQSGMTLDDFVTRANRIPLNATAML
                     RPDAHRLKGEAERGFRTVGTEIREARAAGRAPAACPPERINLDVRQLLAHLNGIPEAR
                     RQRMSVTDGIRSWMRSRYPCPA"
BASE COUNT          471 a          963 c         1075 g          527 t
ORIGIN      
        1 cggcgcgaag cagggcgccg ttgtccttgc tccattcgcg ggtcaggaag atcgcgatct
       61 cggccttggt caaccggtcg tcgcgcgcct gttccagttc gcggttcatg gccttgaacg
      121 cgtccgtgcc ttcggccggc agggcgccgg cggcgtcgcg ggcgctgtcc tggatcgagg
      181 cgatcagccg ctcgcgcagt tcgggggtca gcaccggcgc cggcagctgg cgcgagtagt
      241 atgtgaagct gttgcgatag agcggtccgg tcagttcgct cttcggcggg gctccctcga
      301 aggtataggg cgtcacgtag gggacggccg ggttcgtata gctgaggttg acgacgacct
      361 gcagggtcgt ggcgtcttca gagagcagat agctggtccc gacctcgaca ttgtcgttga
      421 tgacgatggg ggcggttcgc cgctgcacca gcttgacgtc ggaaacggtg acgccactag
      481 cgggctcggc ggcggcctga cggcggaaat gctccaccag cgacgcattg agcgcatcga
      541 ccggcatcac ctcggcgacc tcgtcggcgg cctgggccgc gcggcgcgac ggtccggcgt
      601 tcatcatggc gtcgaacgcc acggagaaca ggacgccgat catcccctgg gtggcgccgg
      661 cggcggtgct gtcctgggcc atccatgcct tgccgacgcg ggtgttgttc tcggacacgg
      721 tgaccggggt cggccccagg gcttcgatat ggctcgcgtt caacggccgg gcgttcggaa
      781 cctgcggaac agacacacag gcggccagag acagcgcggc gagcgcgcag acgacgattc
      841 tcattggtac tcccccatgc cgtccctcca gacagcgacg ggcggaaggc tatttccgga
      901 tggtggccgc ggtcaaccgc aagtcacgga aattcccggc gaacgggtgc ggcaaatcag
      961 tcgtagttgt cgtaccgctg gccctgcgcc agattgccga acttggtcgt attggcgtcg
     1021 aagctcagct tgatgatgcc gatcgggccg tggcgctgct tgccgatgac gacctcggcc
     1081 tgatgttcca gccggtccat gtcctcctgc cactgcaggt gttcgggcga gccctcgcgc
     1141 ggctcggcgc ggcccagata gtagctctcg cggtacacga acatgacgca gtcggcgtcc
     1201 tgctcgatcg agccggattc ccgcaggtcg gacagctgag gccgcttgtc ctcgcgctgt
     1261 tcgacctgac gcgacagctg cgacagggcg atgatcggca ccgacagttc cttggccagc
     1321 gccttcaggg cgccggtgat ctcggatact tcctgcaccc ggttcttctg gttattgccg
     1381 tcgccgacgg tgatgagctg caggtagtcg acgatgatca ggtcgagccc gtgctccatc
     1441 cgcttcagac ggcgggcgcg ggcggccagc ttggagatgg acaggccgcc ggtggcgtcg
     1501 atgaacagcg ggctttcgcc gatctcgacc gcggcctcgc ggatcttgcc gaagtcgcca
     1561 gcatcgattt cgcccttgcg cagcttgtcc gacgacacgc ccgaggcgtc ggccagaata
     1621 cgcatggcca gctgttcggc gctcatttcc agactgtaga aggcgaccac gccgccgttg
     1681 acggtcttgc ggccctcggg cgtcggctcc cagcggtagt tgcgggcgac gttgaaggcg
     1741 atgttggtcg ccagcgccgt cttgcccatc gacggacggc cggcgagtac cagaaggtcg
     1801 gaggggtgca ggccgcccag cttggcgtcc agatcgtcga ggtgggtcgc cagacccgcc
     1861 agtttcccgt cgcgctgata ggcctcggcc gccatctgta cggcgccgga cagcgcactg
     1921 gagaagctga cgaagcccga cgacggcttg cccgtctcgg ccagggtgta gagccgctgc
     1981 tccgcctgtt cgatctggtc gatggcggcg gtctcgggct ggggcgcctc gcgcacgatc
     2041 tcgccaccga tgcggatcag gtcgcggcgc agggccagat cgtagacgac gcgcgcatag
     2101 tcgggggcgt tggccgccgg cggggcgcgg tcgaccagat cggcgaggta gcgcaggccg
     2161 ccgaactcct ggaaggccgg gtcctgtttg aaccgctcca tcaggatggt gggctcggcc
     2221 agcatgccct gacggatgtg gtcctcgatc gcctcataga gccgctggtg gaagggctcg
     2281 tagaaatggc tgccgcgcag ccggtcgctg agccgttcaa acaccgcatt gtcgaacatc
     2341 agcgagccca gcagggcctg ctccgcctcg aggttgtgag gcatcgacgg gatggatccc
     2401 gagtctgcgg gaccggggaa ggcgagcggt gcgaaggcgt tcatggggga catctactgc
     2461 ccttagcagg gccgtacgtc atgtccggtc gtatccgcga ccggggaaaa ccggtcgcct
     2521 ctgtggacgc tccggaattc cgtagccgga tcagttaacg gctgcgctgg aaccggctca
     2581 ggccgggcag ggataccgcg accgcatcca cgaccggatg ccgtcggtga cgctcatccg
     2641 ttggcgccgt gcctcgggaa tgccgttcag atgcgccaga agctggcgta catccagatt
     2701 gatgcgttcc ggcggacagg cggccggcgc gcggccggcg gcccgggctt cccggatctc
     2761 ggttccgacc gttcggaagc cgcgctccgc ctcgcccttc agtcggtgcg cgtcgggacg
     2821 cagcatggca gtcgcattaa gcgggatgcg attggcgcgg gtgacgaagt cgtccagcgt
     2881 catgcccgac tgggcgaagg ccgaaccggc catggccagc gcgaggccgg cgatcaggat
     2941 gatgcgtgtc atgtcttccc ctccgagaca cgcagaatgc tcgggaaatc gtggcgaaac
     3001 aacggtcaac ggcgcgggcg gcgaaaccgc ccgcgc
//
LOCUS       DASVVN010000654         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_341338,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000654 DASVVN010000000
VERSION     DASVVN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341338"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VF686_10720"
     CDS             <1..1017
                     /locus_tag="VF686_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8662327.1"
                     /translation="TPGGSSGGSASAVAADLCLAATASDTGGSIRQPAAFTGTVGIKP
                     TYGRASRYGMVAFASSLDQAGPIAKTVEDSAILLRAMCSFDPKDSTSLDVETPDWTQS
                     LNKGVKGLRIGVPKEYVVDGMPAEIQKLWDQGVEWLKAGGAEIVEISLPHTKYALPAY
                     YIIAPAEASSNLARYDGMRFGHRAEGTTSLTDLYETSRAEGFGKEVQRRLTIGAYVLS
                     AGFYDAYYVRALKVRRRIAMDFDAVWDKVDAILTPSTPSAAFALGDKQIDPLQMYLND
                     IFTVTANLAGLPGISVPAGVDSGGLPLGLQVIGKALDEATVFQVASALEDAAGFVAKP
                     EKWW"
     gene            1020..>2335
                     /gene="gatB"
                     /locus_tag="VF686_10725"
     CDS             1020..>2335
                     /gene="gatB"
                     /locus_tag="VF686_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517891.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8662328.1"
                     /translation="MTETSKLIQGRTGPWEIVMGLEIHAQVASNAKLFSGAAVGFGAG
                     PNEQVSLVDAGFPGMLPTLNRHCVEQAVKTGLGLKAQINRRSQFDRKNYFYPDLPTGY
                     QISQLYFPIVGEGVVEVEAEDGSFFNVGIERLHLEQDAGKLIHDLSPTESYVDLNRAG
                     TALMEIVSRPDIRSPEEAVAYVKKIRTILMYLGTCDGDMEKGNLRADVNVSVCREGQY
                     AKYKATGDFSHLGTRCEIKNVNSFRFISQAINYEARRQIEILEDGGKIDQETRLYDPT
                     AGETRSMRSKEEAMDYRYFPDPDLLPLELEQAWIDDIRANLPELPDEKRRRLMADYGL
                     SQYDAVVLISEQAKADFFEAAAKGRDAKLVSNWVTNEVSARLAADGKDFSEITLPAAH
                     VAQLVELIENGTISSKIAKEVFDHVWNGEGPPAAVVEKHGLKQVTDT"
BASE COUNT          434 a          818 c          730 g          353 t
ORIGIN      
        1 acaccgggcg gcagctcggg cgggtcggcc tcggccgtcg ccgccgacct ctgcctggcc
       61 gccacggcgt cggacaccgg cggctcgatc cgccagcccg cggccttcac cggcacggtc
      121 gggatcaagc ccacctacgg ccgcgcctcg cgctacggca tggtcgcctt cgccagttcg
      181 ctggatcagg ccgggccgat cgcgaagacg gtcgaggact cggccatcct gctgcgcgcc
      241 atgtgctcgt tcgatccgaa ggactcgacc agcctcgacg tcgaaacgcc cgactggacc
      301 caatccctga acaagggcgt caagggcctg cgcatcggcg tgccgaagga atacgtcgtc
      361 gacggcatgc ccgccgagat ccagaagctg tgggaccagg gcgtcgaatg gctcaaggcc
      421 ggcggcgccg agatcgtcga gatcagcctg ccgcacacca aatacgccct gccggcctat
      481 tacatcatcg ccccggccga ggcctcgtcc aacctggccc gctacgacgg catgcgcttc
      541 ggccaccgcg ccgagggcac gaccagcctg accgacctct acgagaccag ccgcgccgag
      601 ggcttcggca aggaggtcca gcgccgcctg acgatcggcg cctatgtcct gtcggccgga
      661 ttctatgacg cctactacgt ccgtgccctc aaggttcgcc gccgcatcgc catggatttc
      721 gacgcggtgt gggacaaggt cgacgccatc ctgaccccgt ccaccccatc ggccgccttc
      781 gcgctgggcg acaagcagat cgatccgctg cagatgtatc tgaacgacat cttcaccgtg
      841 accgccaacc tcgccggcct gccgggcatt tcggtgcccg cgggcgtcga cagcggcggc
      901 ctgccgctcg gccttcaggt catcggcaag gcgctggacg aggcgaccgt cttccaggtc
      961 gccagcgcgc tggaagacgc ggcggggttt gtcgccaaac ccgagaagtg gtggtgagca
     1021 tgaccgagac ctcgaaactc atccagggcc gcaccggccc gtgggaaatc gtcatggggc
     1081 tggagatcca cgcccaggtg gcctcgaacg ccaagctgtt ctcgggcgcg gccgtcggct
     1141 tcggcgcggg gccgaacgag caggtctcgc tggtcgacgc cggcttcccc ggcatgctgc
     1201 cgaccctgaa ccgccactgc gtcgaacagg cggtcaagac cggcctcggc ctcaaggcgc
     1261 agatcaaccg ccgcagccag ttcgatcgga agaactattt ctaccccgac ctgccgaccg
     1321 gctatcagat cagccaactc tacttcccca tcgtcggcga aggcgtcgtc gaggtggagg
     1381 ccgaggacgg ctccttcttc aacgtcggca tcgaacgcct gcatctggag caggacgcgg
     1441 gcaagctgat ccacgacctg tcgccgaccg agagctacgt cgacctgaac cgcgcgggca
     1501 ccgccctgat ggagatcgtg tcgcgtccgg atatccgttc tcctgaagaa gccgtggctt
     1561 acgtcaagaa gatcagaacc attctgatgt atctgggcac ctgcgacggc gacatggaga
     1621 agggcaatct gcgcgccgac gtcaacgtct cggtctgccg cgaggggcag tacgccaaat
     1681 acaaggccac cggcgacttc agccatctgg gcacgcgctg cgagatcaag aacgtcaact
     1741 cgttccgttt catctcacag gccatcaact atgaggcccg gcgccagatc gagatcctcg
     1801 aggacggcgg caagatcgat caggaaaccc gcctctacga cccgacggcc ggcgagaccc
     1861 gctcgatgcg ctccaaggaa gaggcgatgg actatcgcta tttccccgac ccggacctgc
     1921 tgccgctcga gctggagcag gcgtggatcg acgacatccg cgccaacctg cccgaactgc
     1981 ctgacgagaa gcgccgccgg ctgatggcgg actacggcct gtcgcaatac gacgcggtgg
     2041 tcctgatctc ggaacaggcc aaggccgact tcttcgaggc cgccgcgaaa ggccgcgacg
     2101 ccaaactggt gtccaactgg gtgaccaacg aggtttccgc ccgtctcgcc gcggacggca
     2161 aggacttttc ggaaatcacc ctgccggccg cacacgtggc ccagctggtc gagctgatcg
     2221 agaacgggac catctcgtct aagatcgcca aggaggtctt cgaccacgtc tggaacggcg
     2281 agggcccgcc cgccgcggtc gtcgagaagc acggcctgaa acaggtcacc gacac
//
LOCUS       DASVVN010000655         5253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_208709,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000655 DASVVN010000000
VERSION     DASVVN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208709"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VF686_10730"
     CDS             complement(<1..876)
                     /locus_tag="VF686_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX8662329.1"
                     /translation="MKFGIDRLLCEPELHAALKGRRVALLGHPASVTADLTHSLDALM
                     ACDDLNVTAAFGPQHGMRGDLQDNMMESPDFTDPRHGIPVFSLYGEVRRPSGQAMGTF
                     DVLLVDMQDLGCRIYTFVTTLLYVLEESARHGKAVWVLDRPNPAGRPVEGTTLLPGWE
                     SFVGAGPMPMRHGMTLGEMGRWFIDHFKLDVEYRVIEMQGWAPDQGPGFGWPTGERSW
                     INPSPNAANLSMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDARAVIAEMQAFA
                     PQWLAGCRLRDCWFEP"
     gene            complement(873..1442)
                     /locus_tag="VF686_10735"
     CDS             complement(873..1442)
                     /locus_tag="VF686_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX8662330.1"
                     /translation="MQATLIPHPLTPCPAVDRIEVEVERVGEGGLFLAYSLFGSLGDI
                     RLPEATPGPASGPTDELWRHSCFEAFIRNGDADGYLELNFAMSGEWAAYRFSGYREGM
                     TSANVSPHACQGWWGPQPEPLRLEVWIDPGRMTRPDLPASLWRMGLSAVVETTDGAIS
                     YWALAHPPGKPDFHHPDSFALTLPPPEPA"
     gene            1637..2902
                     /gene="tyrS"
                     /locus_tag="VF686_10740"
     CDS             1637..2902
                     /gene="tyrS"
                     /locus_tag="VF686_10740"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX8662331.1"
                     /translation="MTPAPETAFQSDFLRTLQDRGYIHQITHPAELDAAAAAGVVTGY
                     IGFDATAPSLHVGSLIQIMMLRRLQQAGGKPIVLMGGGTTKVGDPTGKDASRPQLTDE
                     TIQANIASIRTVFERFLTFGDGPTDAVMVDNDQWLSTYGYIQFLRDYGTHFTVNRMLA
                     FDSVKLRLEREQPMTFLEFNYMLMQSVDFLELNRARNCTLQMGGSDQWGNIVSGVDLV
                     RRVDQKAAFGLTTPLLTTASGGKMGKTAQGAVWLNAEQLSPYDYWQFWRNVDDADVGK
                     FLRLFTDLPLDEIARLEALEGAGINDAKKTLADAATTMLHGADAASHARGAAEAAFEQ
                     GRLSADLPTVELPRDEVIGAMIAAVTTRAGLTASNGEARRLAQGGGLRLNDEAIADGA
                     RLIEAGDLNADGILKLAAGKKKIVLVRPI"
     gene            2912..3379
                     /locus_tag="VF686_10745"
     CDS             2912..3379
                     /locus_tag="VF686_10745"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8662332.1"
                     /translation="MSEITEGQKAPAFDMATDGGSRASLAALKGQKVVLFFYPKADTP
                     TCTNEAKDFSALADDFAAAGAVVIGVSRDPVRKLDRFKAKYDLKVTLASDEPEDVTDR
                     WGVWVQKKLYGREYMGIERATFLIDGAGGVRRVWRKVSVKGHAAEVLAAVRSL"
     gene            3579..4724
                     /locus_tag="VF686_10750"
     CDS             3579..4724
                     /locus_tag="VF686_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8662333.1"
                     /translation="MTEHEPTVRRSLIGRWFPTRYLYLRDGDAVRAWALTPGKQVVGA
                     VAALLIGGWTLAASGSLIFDMVARSQSDSTVARARAASERMNADLQARLDSAVVRMSA
                     TTGSLDEMAQMVERRHAALTQVMGMFRGVEGAQTALRPAIALDPARSSPVQRMIAVRM
                     DQERLISRAETEAHTRAERLRLAFRLAGLNPAAYSPQGTALGGPLVEARDPRALAAVL
                     DVDEAFAVRIRHAADNLSEMRTLANAAEGMPFRRPTQARATSGFGVRFDPFNGRPALH
                     QGQDFAAPLNTPIYAPAPGIVSFVGVRSGYGNTVEVDHGRGFKTRYAHLNATAVTAGQ
                     RIALGQRVGAMGTTGRSTGVHLHYEVWMDGRPQNPARFMRAGDHLVQ"
     gene            4714..>5253
                     /locus_tag="VF686_10755"
     CDS             4714..>5253
                     /locus_tag="VF686_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8662334.1"
                     /translation="MFNKTKSPVPSPEPRANSGSAIPPLPDLPMPGGSAPAAAPRAAA
                     PAPVSQRGLSTLSSDLQFDGNISGGGDLQIDGAIKGDVRVGRLIVGETGAVEGNVSGD
                     YVEIRGRVVGAISGKQVKLIATAYVDGDITAEQMSIDIGAYFQGRVLQGRREAPAPTA
                     APRPAAPEPAPQIIDMKPSA"
BASE COUNT          866 a         1981 c         1633 g          773 t
ORIGIN      
        1 cggctcgaac cagcagtcgc gcagccggca gcccgccagc cactgcggcg cgaacgcctg
       61 catctccgcg atcacggcgc gggcgtcgat gtccggcgcc ccgaacagct ccagcggccg
      121 cgtcgtgccc cgcccctccg acagggtcgt gccctccagc atcaccgtgc cggcataggc
      181 ccgcgccatc gacagattgg ccgcattggg gctggggttg atccagctgc gctcgccggt
      241 cggccagccg aagcccgggc cctgatccgg cgcccagccc tgcatctcga tgacgcgata
      301 ctccacatcc agtttgaagt ggtcgatgaa ccagcgtccc atctcgccca gggtcatgcc
      361 gtgccgcatt ggcatcggcc cggccccgac gaaactctcc cagcccggca gcagggtggt
      421 gccctccacc ggccgcccgg cggggttggg ccggtccagc acccacaccg ccttgccgtg
      481 cctggctgac tcttccagca cgtacagcag cgtggtcacg aaggtgtaga tgcggcagcc
      541 caggtcctgc atgtcgacca gcaggacgtc gaacgtcccc atcgcctggc cgctggggcg
      601 cctcacctcg ccgtacaggc tgaacaccgg aatgccgtgc cgcggatcgg tgaagtccgg
      661 gctctccatc atattgtcct gcaggtcgcc ccgcatgccg tgctgcggcc cgaaggccgc
      721 cgtcacgttc aggtcgtcgc acgccatcag cgcgtccagc gaatgcgtca ggtccgccgt
      781 gaccgaggcc ggatgcccca gcaaggccac gcgcctgccc ttcaaggccg cgtgcagttc
      841 gggctcgcac aagaggcggt cgatgccgaa tttcatgcgg gctccggggg cggcagggtc
      901 agggcgaacg agtcgggatg atggaagtcc ggcttacccg gcgggtgggc caaggcccag
      961 taggagatgg cgccgtccgt agtctcgacc acggccgaca gtcccatacg ccacagcgac
     1021 gccggcaggt ccgggcgcgt catgcgcccc ggatcaatcc agacttccaa cctcagcggc
     1081 tccggctgcg gcccccacca tccctggcag gcgtgggggg aaacattggc ggaggtcatg
     1141 ccctcacgat agccgctgaa acgataggcc gcccactcgc ccgacatggc aaaattcaat
     1201 tccagatagc cgtccgcgtc gccgttgcgg atgaaggcct cgaaacaact gtgccgccac
     1261 agctcatcgg tcggccccga tgcgggtccc ggcgtcgcct ccggcaggcg gatatcgccc
     1321 agcgacccga acaggctgta ggccagaaac agcccgcctt cgcccacccg ctcgacctcg
     1381 acctcgatac ggtcgacggc ggggcacggc gtcagggggt gcgggatcag ggtcgcctgc
     1441 atcggtcttg gatagcgacc gcttgacgcc ggtcaatggc cgacgctacc caggcaccat
     1501 gaccgccgtc cgcgccctct ccggcctgta ttaccgcccg cacctctagc gagcggcccg
     1561 cgatcccctg cgccgcacct tcgccggcgc gcaaactccc atgacgcccg ccggcatccc
     1621 tgctaacgga gccgccatga cccccgcccc cgagacagcc ttccagtccg acttcctgag
     1681 aaccctccag gaccgcggct acatccatca gatcacccac ccggccgagc tggacgccgc
     1741 ggccgccgcc ggcgtggtca ccggctacat cggcttcgac gccacggccc cgtcgctgca
     1801 cgtcggcagc ctgatccaga tcatgatgct gcgccgtctc cagcaggcgg gcggtaagcc
     1861 catcgtcctg atgggcggcg gcaccaccaa ggtcggcgac ccgaccggca aggacgcctc
     1921 gcgcccccag ctgaccgacg agaccattca ggcgaacatc gcctcgatcc gcaccgtgtt
     1981 cgaacgcttc ctgaccttcg gcgacggtcc gaccgacgcg gtcatggtcg acaatgacca
     2041 gtggctgtcg acctacggct acatccagtt cctgcgggac tacggcacgc atttcaccgt
     2101 caaccgcatg ctggccttcg actcggtcaa gctgcggctg gaacgcgaac agccgatgac
     2161 cttcctcgag ttcaactaca tgctgatgca gtcggtggac ttcctcgaac tgaaccgcgc
     2221 ccggaactgc accctgcaga tgggcggctc ggaccagtgg ggcaacatcg tctcgggcgt
     2281 cgatctggtg cgccgcgtgg atcagaaggc cgccttcggc ctcaccacgc ccctgctcac
     2341 caccgcttcc ggcggcaaga tgggcaagac ggcccagggc gcggtctggc tgaacgccga
     2401 acagctgtca ccctacgact actggcagtt ctggcggaac gtcgacgacg ccgacgtggg
     2461 caaattcctg cgcctgttca ccgacctgcc gctggacgag atcgcccggc tggaggcgct
     2521 cgagggcgcc ggcatcaacg acgccaagaa gaccctcgcc gacgccgcca cgaccatgct
     2581 gcacggcgcg gacgcggcgt cgcacgcccg cggcgcggcc gaggccgcct tcgaacaggg
     2641 cagactctcc gccgacctgc cgaccgtcga actgccgcgt gacgaggtca tcggcgccat
     2701 gatcgccgcc gttacgaccc gggccggcct gaccgcctcc aacggcgaag cccgccgcct
     2761 cgcccagggc ggcggcctgc gcctcaacga cgaagccatc gccgacgggg ctcgcctgat
     2821 cgaggccggc gacctcaacg ccgacgggat tctgaagctg gccgccggca aaaagaagat
     2881 cgtcctcgtc cgcccgatct gaaagcgaac catgtccgag atcaccgaag gccagaaggc
     2941 ccccgcattc gacatggcca cggacggcgg cagccgcgcg tcgctggccg cgctcaaggg
     3001 ccagaaggtc gtcctgttct tctaccccaa ggcagacacc ccgacctgca ccaacgaggc
     3061 gaaggacttc tcggccctgg ccgacgactt cgccgccgcc ggcgccgtgg tcatcggcgt
     3121 ctcccgcgac ccggtcagaa agctcgaccg gttcaaggcc aaatacgatc tcaaggtcac
     3181 cctcgcctcc gacgaacccg aagacgtcac cgaccgttgg ggcgtctggg tgcagaagaa
     3241 gctctacggt cgcgaataca tgggcatcga gcgcgccacc ttcctgatcg acggcgcggg
     3301 cggcgtccgc cgggtctggc gcaaggtctc agtcaagggc cacgccgccg aagtcttggc
     3361 ggccgttcga tcactctgac cacggacgtt tctcgacaac cacagatgtc atctttgttt
     3421 gatagattcc cgcgctcgcc cctcaggcga gtttcggtcc cgaataccgt cttcattccc
     3481 gcgaaaccgg gcgatcatgg ttaacaaacc gtgaagagac ttgtcgtccc tgcagtaccg
     3541 ggcgcataac cccgcctcgt acgtgggggc gttgcgtgat gaccgaacac gagccgactg
     3601 tcaggcggtc tctgatcgga aggtggtttc cgacccgata tctgtatctg cgcgacggcg
     3661 acgccgtccg cgcctgggcc ctgacgcccg gcaaacaggt cgtgggggcc gtcgcggctc
     3721 tcctgatcgg cggttggacg ctggcggcct cgggcagtct gatcttcgac atggtggcgc
     3781 gcagtcagtc cgacagcacg gtcgcccgcg cccgcgccgc ctccgagcgg atgaacgccg
     3841 acctgcaggc ccgtctggac agcgccgtgg tccgcatgag cgccacgacc ggctcgctgg
     3901 acgaaatggc ccagatggtc gagcgccgcc acgccgccct gacccaggtc atgggcatgt
     3961 tccgcggcgt cgagggcgcc cagacggcct tgcgccccgc gatcgccctg gatcccgccc
     4021 gatcctcgcc cgtccagcgc atgatcgccg tgcgcatgga tcaggagcgg ctgatctcgc
     4081 gcgccgagac cgaggcccac acccgggccg agcgcctgcg cctcgccttc cggctggcgg
     4141 gcctgaaccc cgccgcctat tcaccgcagg gcacggcgct gggcggcccg ctggtcgagg
     4201 cccgcgatcc tcgcgccctc gccgccgtac tggacgtgga cgaggctttc gctgtccgaa
     4261 ttcggcacgc cgccgacaat ctgtccgaga tgaggacact cgccaacgcc gccgagggca
     4321 tgcccttccg ccgccccacg caggcccggg ccacctcagg cttcggcgtg cgcttcgacc
     4381 ccttcaacgg ccggcccgcc ctgcatcagg gtcaggattt cgccgccccc ctcaacaccc
     4441 ccatctacgc ccccgctccc ggaatcgtgt ctttcgtcgg cgtgcgttca gggtatggca
     4501 acacggtcga ggtagaccat ggccgaggtt tcaagacgcg atacgcccat ctgaacgcca
     4561 cggcggtgac ggctggccag agaattgcac tgggccagcg cgtcggcgcc atgggcacga
     4621 caggacgctc caccggcgtt cacctgcact acgaagtgtg gatggacggc cgcccgcaaa
     4681 accccgcccg cttcatgaga gccggagacc atcttgttca ataaaaccaa atcccccgtc
     4741 ccgtcccccg agccccgcgc caacagcggc tcggccattc cgcccctgcc cgatctgccc
     4801 atgcccggcg gttccgcccc ggccgccgct ccgcgcgccg ccgccccggc gcccgtcagc
     4861 cagcgcggcc tgtcgaccct gtcgtcggac ctccagttcg acggcaatat ctcgggcggc
     4921 ggcgacctcc agatcgacgg cgccatcaag ggcgacgtgc gcgtcggccg tctgatcgtc
     4981 ggcgagaccg gcgcagtcga aggcaatgtc tcgggcgact atgtcgagat ccgcggccgc
     5041 gtcgtcggcg ccatctcggg caaacaggtc aagctgatcg ccacggccta tgtcgacggc
     5101 gacatcaccg ccgagcagat gtcgatcgac atcggcgcct atttccaggg ccgcgtcctg
     5161 cagggccgcc gcgaggctcc ggcccccacg gccgcacccc gcccggccgc gcccgagccc
     5221 gcgccgcaga tcatcgacat gaagccgtcg gcc
//
LOCUS       DASVVN010000656         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_51704, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000656 DASVVN010000000
VERSION     DASVVN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51704"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF686_10760"
     CDS             <1..429
                     /locus_tag="VF686_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8662335.1"
                     /translation="GFCDTLILSYAAKYASAFYGPYRDAIGTLGLLKGDKATYQMDPA
                     NTHEALREVELDLAEGADMIMVKPGLPYLDIVRRLVETFAVPTFAFQVSGEYAMIKAA
                     AERGWLDETRAILESLTAFKRAGAAGVLTYFAPTAARLLG"
     gene            487..1239
                     /locus_tag="VF686_10765"
     CDS             487..1239
                     /locus_tag="VF686_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8662336.1"
                     /translation="MPVVFITGASAGFGVAFARRFAGEGWKVVATARRTDRLQALADE
                     IGPALLPLELDVQDREAVYTAFDRLPEGHREVDVLINNAGLALGLEPAQRAAPDDWDQ
                     MIDTNIRGLVHCTRAALPGMVDRRRGHVINIGSVAANYPYPGGNVYGGSKAFVRQFSL
                     NLRSELHGTGVRVTCIEPGMVAGTEFSPVRFRGDLERAEGVYRGANALTPEDIAETVW
                     WSASLPPHVNINTLEVMPVTQSWAGFQVHREA"
     gene            1301..2176
                     /locus_tag="VF686_10770"
     CDS             1301..2176
                     /locus_tag="VF686_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX8662337.1"
                     /translation="MALGEQPIVIKKVKKVVGHGHHGGAWKVAYADFVTAMMAFFLLM
                     WLINTTSPEQKKGIADYFAPATVSPSTSGSGGILGGASLGEDGNLSDGSTTVVEQPPE
                     APTKGDGVQSDSAAEQAMKEALARRDEASFQSAAASLRQAMQDLPELAELSKHLIIDE
                     TPEGLRIQLVDQEGRSMFAEGSARPNDRARILLKAIAGVVNQLPNRVTITGHTSASVS
                     RRAEGDWGLSSARADASRQILQGSGVDPDRFFQVAGRAASEPLYPDDPTLAGNRRIAL
                     VLLREAPVLPPGHAL"
     gene            2248..>2443
                     /locus_tag="VF686_10775"
     CDS             2248..>2443
                     /locus_tag="VF686_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX8662338.1"
                     /translation="MTQHFPTRQLRFFLTAPQPCPYLPGKEERKVFTHIIGRRGRELN
                     EILTQGGFRRSQTIAYRPACD"
BASE COUNT          386 a          904 c          790 g          363 t
ORIGIN      
        1 ggattctgcg acaccctgat cctgtcctac gccgccaagt acgcctctgc cttctacggt
       61 ccctaccggg acgccatcgg cacgctgggc ctgctcaagg gagacaaggc cacctaccag
      121 atggacccgg ccaacaccca cgaggcgctg cgcgaggtgg agctcgacct ggcggagggc
      181 gcggacatga tcatggtcaa gccgggcctg ccctacctcg acatcgtgcg ccgcctggtc
      241 gagaccttcg ccgtccccac cttcgccttc caggtctccg gcgagtacgc gatgatcaag
      301 gccgccgccg agcgcggctg gctcgacgag acgcgcgcca tcctggagag cctgaccgcg
      361 ttcaagcggg ccggcgccgc gggcgtcctc acctacttcg cgccgacggc ggcccgcctg
      421 ctcggctgac gcattgcccg cgccgcggct tcgcgccaga tgggccgcct gagccaggga
      481 gtgtccatgc ccgtcgtctt catcaccggc gccagcgccg gcttcggcgt cgccttcgcc
      541 cgccgcttcg ccggcgaggg ctggaaggtg gtcgccaccg cccgccgcac cgaccgcctg
      601 caggcgctcg ccgacgagat cggacccgcc ctgctgccgc tggagctcga cgtgcaggac
      661 cgcgaggccg tctacaccgc cttcgatcgc ctgccggagg gtcatcgcga ggtcgacgtc
      721 ctgatcaaca acgccggcct ggcgctcggc ctggagccgg cccagcgcgc cgccccagac
      781 gactgggacc agatgatcga caccaacatc cgggggctgg tccactgcac ccgcgccgcc
      841 cttcccggca tggtcgatcg ccggcgcggc cacgtcatca acatcggctc cgtggctgcg
      901 aactatcctt atcctggcgg aaatgtttat ggtggctcaa aggctttcgt ccggcagttc
      961 agcctgaacc tgagaagcga gctgcacggg actggcgtcc gggtgacctg catcgagccg
     1021 gggatggtgg ccgggacgga gttctcgccc gtgcgcttcc gcggcgacct ggagcgggcc
     1081 gagggggtgt atcgcggggc caacgccctc acccccgagg atatcgccga aacggtctgg
     1141 tggtccgcct ccctgccgcc ccacgtgaat atcaacaccc tggaagtgat gccggtcacc
     1201 cagagctggg ccggattcca ggtccaccgc gaggcctagg gcaaccgcat cttaactacg
     1261 cccccgccaa ggtgcgccgt ctcggacaag gcgtgcgatc gtggccctcg gcgaacagcc
     1321 tatcgtcatc aagaaggtga agaaggtggt cggccacggc caccacggcg gcgcctggaa
     1381 ggtcgcctac gccgacttcg tgaccgcgat gatggccttc ttcctgctga tgtggctgat
     1441 caacaccacc agcccggagc agaagaaggg catcgccgac tacttcgccc cggccaccgt
     1501 aagcccctcg accagcggct ccggcggcat cctgggcggc gcctcgctgg gcgaggacgg
     1561 caacctgagc gacggctcga ccaccgttgt cgagcagccg cccgaggcgc ccaccaaggg
     1621 cgacggcgtg cagtccgact ccgcggccga gcaggccatg aaggaagcgc tggcgcggcg
     1681 tgacgaggcc tcgttccagt ccgccgccgc ctcgctgcgc caggcgatgc aggacctgcc
     1741 ggagctggcc gagctctcca agcacctgat catcgacgag accccggaag gcctgcgcat
     1801 ccagttggtc gaccaggagg gccgctcgat gttcgccgag ggctccgccc gcccgaacga
     1861 tcgcgcccgc atcctgctca aggcgatcgc cggggtggtc aaccagctgc ccaaccgggt
     1921 gaccatcacc ggccacacca gcgcctcggt cagccgccgc gccgaaggcg actgggggct
     1981 gtccagcgcc cgcgccgacg cttcacggca aatccttcaa ggatccggcg tcgatcccga
     2041 ccgcttcttc caggtggccg gccgcgcggc gtccgagccg ctttatccgg acgatcccac
     2101 gttggccggt aaccggcgga ttgcattggt gcttctccgg gaagcgccgg tgctgccgcc
     2161 aggtcacgcc ctctaggggc ttgcggggag cgggggcatg atcgcaaatt cgatttccgc
     2221 agcctgcgag actcacggcc caccccggtg acccagcact ttccaacccg ccagctgcgc
     2281 ttcttcctga ccgcgcccca gccctgcccc tacctgccgg gcaaagagga gcggaaggtg
     2341 ttcacccaca tcatcggacg tcggggtcgg gagctgaacg agatcctgac ccagggcggc
     2401 tttcgccggt cgcagaccat cgcctatcgg ccggcctgcg aca
//
LOCUS       DASVVN010000657         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_446029,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000657 DASVVN010000000
VERSION     DASVVN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446029"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VF686_10780"
     CDS             complement(<1..1374)
                     /locus_tag="VF686_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662339.1"
                     /translation="MSGATVSKRVLRTTVAAAAAGAVAFAPMAPLAQEAGAALDPISI
                     RIGANAEFTRLEFAGVIGARSRVRREGRDVIVRIGSTAAPDVSRLKVDPPKGVEKVET
                     RAVQGATELVITLAEGADARTGSADGAVYVNLFASAPDAPAPAVVAASNVAVTTRATA
                     AKVSMTFQWDKPVGAAVFRRGAAVWVVFDTPARLDMSGARQLGPVSDAHWTAAPDHVA
                     VRIAAPEGLAVSAVAEGSNWTVVIGGQPVAVAGVPVERDDSGEPVLVARMAGATKAIW
                     LTDPLAGDRFAAVTALAPGKGFADRRRTVDMALLPTAQGLAVEAASEDLGILAETDRV
                     TFSRPNGLILSPPSAALDAAADESGAPKKAKFPALILSEWADVGHAGFSARYRQLQDA
                     AALEAMAAGDDPRAPAEARFAFARFLVGSGLGYEAVGVLNSIVAKAPNMAGEPELRGL
                     RGAARASI"
     gene            complement(1412..1909)
                     /locus_tag="VF686_10785"
     CDS             complement(1412..1909)
                     /locus_tag="VF686_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8662340.1"
                     /translation="MEALVEAVLKSASDHGVTLSTAESCTGGLIASMLTDVEGRSHAF
                     ERGYVVYTDDAKVEELGVPRRLIDHNGAVSEPVARAMAEGALRDSRAGVAVSVTGFAG
                     PAGPNDEPGLVHFALAATGAPTVHEEHHFGDRGRGPVRLECLRVALRMMRDALPGEGG
                     TVTNR"
BASE COUNT          272 a          689 c          708 g          248 t
ORIGIN      
        1 gatggaggcg cgggcggcgc cgcgcagacc gcgcagctcg ggctcgccgg ccatgttcgg
       61 ggccttggcg acaatgctgt tcagcacgcc cacggcctcg tagcccagcc ccgagccgac
      121 aaggaagcgg gcgaaggcga aacgcgcctc ggccggggcc cggggatcgt cgccggcggc
      181 catggcctcc agtgcggcgg cgtcctgaag ctggcgatag cgggccgaga agccggcgtg
      241 gccgacatcg gcccattcgg acaggatcag ggcagggaat ttcgccttct tcggcgcccc
      301 gctctcgtcg gcggcggcgt ccagggcggc cgatggcggc gacaggatca ggccgttggg
      361 ccggctgaag gtgacgcggt ccgtctcggc caagatgccg agatcctccg acgcggcttc
      421 gacggccagt ccctgcgccg tcggcagcag ggccatgtcc accgtgcggc ggcggtcggc
      481 gaatcccttg ccgggagcca aggccgtaac ggcggcgaaa cggtcgccgg ccagggggtc
      541 ggtcagccag atcgccttgg tcgccccggc catccgggcg accagtacgg gctcgccgct
      601 atcgtcgcgt tcgacaggca cgccggcgac ggcgaccggc tggccgccga tgacgacggt
      661 ccaattcgag ccctcggcga ccgcggagac cgccagccct tccggcgcgg cgatgcggac
      721 ggcgacgtgg tcgggcgcgg cggtccagtg ggcgtcggag accggcccca gttgccgggc
      781 gccggacatg tcgagccgtg cgggggtgtc gaagaccacc cagaccgccg cgccgcgccg
      841 gaagacggcg gcgccgaccg gcttgtccca ctggaaggtc atcgagacct tggcggcggt
      901 ggcgcgggtg gtcaccgcga cattcgaagc cgcgacgacg gccggcgccg gggcgtctgg
      961 ggccgacgcg aacaggttca catagaccgc gccgtcggcc gaaccggtgc gggcgtcggc
     1021 gccctcggcc agggtgatga ccagttcggt ggcgccctgg acggcgcggg tctcgacctt
     1081 ttccaccccc ttgggcggat cgaccttcag gcgcgagacg tcgggcgcgg cggtcgaacc
     1141 gatgcggacg atgacgtcgc ggccctcgcg ccggacccgc gatcgagccc cgatgacgcc
     1201 ggcgaattcc agacgggtga actcggcgtt ggccccgatg cggatcgaga tcggatcgag
     1261 ggcggccccg gcctcctggg ccagcggagc catgggcgcg aacgcgaccg cgcccgccgc
     1321 ggcggcggcc acggtggttc tgagaacgcg cttgctgacg gttgccccgg acatacgcgc
     1381 gcaccctcgc gcgaccggcc tttcaacgcg gttaacggtt ggtgaccgtt ccgccttcgc
     1441 ccggcaaggc gtcccgcatc atccgcagcg ccacccgcag gcattccaga cgcaccgggc
     1501 cacgcccccg gtcgccgaaa tgatgctcct catgcaccgt cggcgcgccg gtcgcggcga
     1561 gcgcgaaatg caccagcccg ggttcgtcgt tcggtccggc cgggcccgcg aaaccggtga
     1621 cggaaaccgc cactcccgcg cgcgaatccc tcagcgcgcc ctcggccatc gcccgggcca
     1681 cgggctcgga caccgcgccg ttgtgatcga tcagccgacg gggaacgccc agttcctcga
     1741 ccttggcgtc atcggtgtag acgacatagc cgcgctcgaa ggcgtgggac cggccctcga
     1801 cgtcggtcag catcgaggcg atcaggccgc ccgtgcagct ttccgccgtg gacagggtga
     1861 cgccgtggtc ggaggcggat ttcagcaccg cctcgaccag cgcctcgatg tcggccg
//
LOCUS       DASVVN010000658         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_208801,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000658 DASVVN010000000
VERSION     DASVVN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208801"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1084
                     /locus_tag="VF686_10790"
     CDS             152..1084
                     /locus_tag="VF686_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8662341.1"
                     /translation="MKKPAVSLTRRLSAAGLALGAAVAVLGMAGSASAEITLQFGFQD
                     RDVARMVSLGGSCNRCELSGRDLTGAAFTGAVFTNATLVGANLRGAELQNSNFTGSDF
                     SRADLTGAEMIAANFTGANFTNANMSGAEISAAILVRARMAGTDLSGAELNNANMNGA
                     VLTGADLSGAEMNATTLANVNARSANMQNAEIAMSNLTNGDFRNVSFRDSTLNGARLT
                     GGNFAGADFRNAEMHRADIRGADLSRARGLTQEQVQRACGDAHTRLPGNLTPRLCRGF
                     AGVRVTPHPRAMPAPPAPPVPPRVRNIVVASSGH"
     gene            complement(1146..>2878)
                     /gene="aspS"
                     /locus_tag="VF686_10795"
     CDS             complement(1146..>2878)
                     /gene="aspS"
                     /locus_tag="VF686_10795"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX8662342.1"
                     /translation="GSNVRLSGWVHRKRDHGGLLFIDLRDHYGLTQLVLGPDTPGFTA
                     SERLRAESVIRVDGTVVARSAETINANLPTGQVEVQVSSVEVLSEAAELPLPVFGEPE
                     YPEEIRLKHRYLDLRRETLHKNIVLRSRVISSIRQRMVGQGFLEYQTPILTASSPEGA
                     RDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSLEFYQ
                     LDVEMSFVTQEDVFAAIEPVMHGVFEEFADGMKVDPYPFVRIPYRESISKYGTDKPDL
                     RNPIEMQDVSAHFRDGGFGLFNKILAGDAKNAVWGIPAPKGGSRAFCDRMNSWAQGEG
                     QPGLGYIFWSEDQGAWGGPIAKNLGPEATDGLMKALGMGQGDAAFFAAGDPAVFHKFA
                     GNARTKLGQDLNLIPDDEFRFCWIVDFPMFEWSEEEKKVDFSHNPFSMPQGEMEALTT
                     KDPLDILAYQYDIVCNGYELCSGAIRNHKAEIMLKAFEIAGYDASVVEDQFGGMLNAF
                     RYGAPPHGGLAPGIDRIVMLLANQVAIREVIAFPLNQQGQDLLMSAPSDAAERQYKEL
                     HIRAALPLKT"
BASE COUNT          481 a          944 c          985 g          468 t
ORIGIN      
        1 cacagggccg gttgaaccag gcctgcggcg acgaagccac gcgcctgccc cgcggcctgt
       61 cgatccccca ctgccaagct gactgaatgc agccccgcac cttaccgcga tttaagtagg
      121 gtttgtgggg gtttggcgtc atgacagggg tgtgaaaaaa cctgccgttt ccctgacccg
      181 tcgcctctcc gccgccggcc ttgcgctggg tgcggcggtg gccgtcctcg ggatggccgg
      241 atcggcaagt gcggaaatca cgctccagtt cggattccag gaccgcgacg tggcccggat
      301 ggtgtcgctg ggcggttcct gcaaccgctg cgaactgtcc ggacgcgacc tgacgggcgc
      361 ggccttcacc ggcgccgtct tcaccaacgc gaccctggtc ggcgccaatc tgcgcggcgc
      421 cgaactgcag aactccaact tcaccggcag cgacttcagc cgggccgacc tgaccggcgc
      481 ggaaatgatc gccgccaact tcacaggcgc gaactttacc aatgcgaaca tgtccggcgc
      541 cgagatctcc gccgcgatcc tggtccgggc gcgcatggcc gggactgatc tgagcggcgc
      601 cgagctgaac aacgccaata tgaacggcgc ggttctgacg ggtgccgacc tgagcggcgc
      661 cgaaatgaac gccaccacgc tggcgaacgt caacgcccgg tccgcaaaca tgcagaacgc
      721 cgaaatcgcc atgtcgaatc tgacaaacgg cgatttccgg aacgtctcgt tccgcgactc
      781 caccctgaac ggagcgcgac tgaccggcgg aaacttcgcg ggcgcggact tccgcaatgc
      841 cgagatgcac cgcgccgaca ttcgcggcgc cgacctgtcg cgcgcgcggg gtctgacgca
      901 ggagcaggtc cagcgggcct gcggcgacgc ccacacccgc ctgcctggca acctcacgcc
      961 acggctctgc cgcggcttcg ccggcgtccg ggtcacgccg cacccgcgcg ccatgccggc
     1021 gccgcccgca ccgccggtgc cgccccgggt ccgcaatatc gtggtcgcca gttcgggcca
     1081 ctgacgacaa aggccgccgt ccccggggac agcggccttt ttcatgttgg cactgaacca
     1141 gcggctcagg tcttcagcgg cagggccgca cggatgtgca gctccttgta ctgacgctcg
     1201 gccgcatccg acggcgcgct catcagcaga tcctgaccct gctggttcag cgggaaggcg
     1261 atgacctcgc ggatcgcgac ctggttggcc agcagcatga cgatgcggtc gatgccgggg
     1321 gccaagccgc cgtgcggggg cgcgccatat cggaaagcgt tcagcatgcc gccgaactgg
     1381 tcctcgacca ccgaggcgtc gtagccggcg atctcgaagg ccttgagcat gatctcggcc
     1441 ttgtggttcc ggatcgcgcc cgagcacagc tcatagccgt tgcagacgat gtcgtactgg
     1501 taggcgagga tgtcgagcgg gtccttggtc gtcagcgcct ccatctcgcc ctgcggcatg
     1561 gagaaggggt tgtgcgagaa gtcgaccttc ttctcttcct cgctccattc gaacatcggg
     1621 aagtcgacga tccagcagaa gcggaattcg tcgtccggga tcaggttcag gtcctgcccc
     1681 agcttggtgc gggcgttacc cgcaaacttg tggaacacgg ccgggtcgcc ggcggcgaag
     1741 aaggcggcgt cgccctggcc catgcccagc gccttcatca ggccgtcggt cgcctcgggg
     1801 ccgaggtttt tggcgatcgg gccgccccag gcgccctggt cctcggacca gaagatgtag
     1861 ccgaggcccg gctggccctc gccttgggcc caggagttca tgcggtcgca gaaggcgcgc
     1921 gagcccccct tgggcgccgg aatgccccag acggcgttct tcgcatcgcc ggccaggatc
     1981 ttgttgaaca ggccgaagcc gccgtcgcgg aagtgcgcgc tgacgtcctg catctcgatc
     2041 gggttgcgca ggtcaggctt gtcggtgcca tatttggaaa tgctctcgcg gtaggggatg
     2101 cggacgaagg ggtagggatc gaccttcatg ccgtcggcga attcctcgaa cacgccgtgc
     2161 atgacgggct cgatcgcagc aaaaacatcc tcttgcgtga cgaagctcat ctcgacatcg
     2221 agctggtaga actccagcga gcggtcggcg cgcaggtcct cgtcgcggaa gcagggcgca
     2281 atctggaaat agcggtcgaa gcccgagacc atcagcagct gcttgaactg ctgcggggcc
     2341 tgcgggaggg cgtagaattt gcccgggtgc agacgcgacg ggaccaggaa gtcacgggcg
     2401 ccttcggggc tggaagccgt caggatcggc gtctggtact cgaggaagcc ctggccgacc
     2461 atgcgctggc ggatcgagga gatcacgcgc gagcgcagga cgatgttctt gtgcagggtc
     2521 tcgcggcgca gatcgaggta gcggtgcttg aggcggattt cctcgggata ttcgggctcg
     2581 ccgaagacgg gcagcggcag ttcggcggct tccgacagca cctcgaccga ggagacctgg
     2641 acctcgacct ggccggtggg caggttggcg ttgatggtct cggccgagcg ggcgacgacc
     2701 gtgccgtcga cgcggatcac gctctcggcg cgcaggcgct cgctggcggt gaaaccgggg
     2761 gtgtcgggac ccagcaccag ctgggtcagg ccgtagtggt cgcgcaggtc gatgaacagc
     2821 aggccgccgt ggtcgcgctt gcgatggacc cagccggaca ggcggacatt ggagcccg
//
LOCUS       DASVVN010000659         5350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_51909, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000659 DASVVN010000000
VERSION     DASVVN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51909"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VF686_10800"
     CDS             complement(<1..558)
                     /locus_tag="VF686_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8662343.1"
                     /translation="MTGSAVAQDRTPDIMVSTWDFGAAANAAGWTAREGGGSALDMVE
                     AGGRVAEADESNSSVGLGGLPDRDGRVTLDACVMTWAGDIGAVCALEDFVHAVSVARR
                     VMERTPHTMLVGAGARSFAIDQGFETQNLLTPRAEAAWREWLKTANYAPRPNSENNDW
                     RATPGGPGNHDTIGLLAIGADQRMAG"
     gene            complement(606..1322)
                     /gene="radC"
                     /locus_tag="VF686_10805"
     CDS             complement(606..1322)
                     /gene="radC"
                     /locus_tag="VF686_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX8662344.1"
                     /translation="MLDQPKTPHHAGHRERLRERARGAGLHHLPDYELLELFLFRSQP
                     QGDVKPVAKALLARFGSLAAVLAASVEDLMTVRAEDAKGRSKGVGAETALDLAALHEV
                     ARRVTREEAAARPVISSWTALVAYVRMSLQHEPREQFRVLYLDNRNQLILDEIQNRGT
                     VDHAPVYPREVVRRALELSAKSMIIVHNHPSGDPTPSRPDIDMTRQVVEAARALGIDV
                     HDHLIVGRAGVASFKQLGLL"
     gene            complement(1352..2173)
                     /gene="map"
                     /locus_tag="VF686_10810"
     CDS             complement(1352..2173)
                     /gene="map"
                     /locus_tag="VF686_10810"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX8662345.1"
                     /translation="MYETPELDDDAFVRTHEIRVHDAEAFEGMRRAGRLVAEALDMIT
                     PFVVPGVTTGELDDRIREFTLDHGALPACLGYKGYEKTICTSINHVVCHGIPGDKVLK
                     DGDIVNIDHTVIVDGWHGDSSRMYAVGNINARAKKLIDVTYDSLDAGLAVIKPGNTFG
                     DIGYAIQQVVEANRMSVVRDFCGHGIGRLFHDSPNVLHYGRRGEGAVLKTGMFFTVEP
                     MVNLGKPHVKVLSDGWTAVTRDKSLSSQCEHTIGVTEDGLEIFTASPAGLFRPKF"
     gene            complement(2229..3314)
                     /locus_tag="VF686_10815"
     CDS             complement(2229..3314)
                     /locus_tag="VF686_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX8662346.1"
                     /translation="MIDRRSLLAAPFALAACNRFASAGTPGPEAASLKDLASFPIGAT
                     GMTGQLSDPVWVAQMRKHVSQLTPEWELKPERILQPDFTYDFSASDRIVDWGREQGMR
                     VHGHTLVWYSQGQDAFTDVLPRARFGAEYDRYVSTVAGRYRGRMAGWDVVNEPVAEDG
                     DGLRDCHFSRALGQEGYIVRAFEQARAADPDAVLFLNEYNLENIPRKGATFLRLVERL
                     LTLGAPIGGIGTQSHLDIEIPAGQIRTFIKDAGSLGLPIHVSELDFSMRSDNRLDTRS
                     LAAKRAQQVARVGELAEAFMALPERQRYAFTTWGLRDTDSWLLRPEGKGWTDDSPLLL
                     DAKGRANPAYAAATQAFAGRPAASTRP"
     gene            complement(3337..3747)
                     /locus_tag="VF686_10820"
     CDS             complement(3337..3747)
                     /locus_tag="VF686_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX8662347.1"
                     /translation="MTAWAQSLIRISNYEVETLQKRLAEITARRASAEMRIAVLEAEA
                     EIEQERARSDADAAMMLQAYLSGWKLRKGAAEADVVVISAEEEGARDALTGAFEELKK
                     FEHVAETTRLNSIIAAGKRETAAFDELGLRRRAG"
     gene            complement(3761..5101)
                     /gene="fliI"
                     /locus_tag="VF686_10825"
     CDS             complement(3761..5101)
                     /gene="fliI"
                     /locus_tag="VF686_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX8662348.1"
                     /translation="MRNLAAAVEAVDPLVVTGKVAGVNGLLIESRGGLSRLAVGARAE
                     IERRHLHPLPAEVVGFRDAKALLMPFGPVEGVAPGADIRIVSAAASVRPTTAWLGRII
                     DAFGQPIDGKGPLPQGPAAYPLRAPPPPAHSRGRVGERLDLGVRSMDVFTTTCRGQRL
                     GIFAGSGVGKSVLLSMLARQATCDVVVVGLIGERGREVREFIEETLGEEGLRRAIVVV
                     ATSDEPALKRRQAAYMTMAISEYLRDQDLEVLCLMDSVTRFAMAQREIGLAAGEPPTT
                     KGYTPTVFTELPKLLERAGPGPVRPDGTTAGPITALFTVLVDGGDHDEPIADAVRGIL
                     DGHIVMDRKIAERGRFPAIDVLKSVSRTLPACQTPPERELNKRARQCLSAYANMEELI
                     RIGAYRTGADPIVDRAIALNPALENFLGQDKDDHTSLSDSFTQLEAILNQGMIQ"
BASE COUNT          798 a         1684 c         1983 g          885 t
ORIGIN      
        1 gccggccatc cgctggtccg cgccaatggc cagcaggccg atggtgtcgt ggtttcccgg
       61 cccgcccggg gttgcgcgcc aatcgttgtt ctcgctgttg ggccgcgggg cgtagttcgc
      121 cgtcttcagc cattcgcgcc aggccgcctc ggcgcggggg gtcagaaggt tttgcgtctc
      181 gaagccctga tcgatggcga atgaccgcgc acccgcgccg accagcatgg tgtggggcgt
      241 gcgctccatg acccggcggg cgacggagac ggcgtggacg aagtcctcga gcgcgcagac
      301 ggccccaatg tcgcccgccc aggtcatgac gcaggcgtcc agcgttaccc ggccgtcgcg
      361 gtcaggcagg ccgcccagac cgacggagga gttgctctcg tcggcctcgg ccacgcggcc
      421 gccggcctcg accatgtcca gcgccgagcc accgccctca cgcgcggtcc agccggcggc
      481 gttggccgca gcgccgaagt cccaggtgga gaccatgatg tccggcgtcc ggtcctgcgc
      541 aaccgcggat ccggtcatgg cgacaagcgg cgtggccgcc aacagcgacc tgcgcgaggg
      601 cccgctcaca gcaggcccag ttgcttgaag ctggccacgc cggcgcgacc gacgatcagg
      661 tggtcgtgga cgtcgatgcc cagggcgcgc gccgcctcca cgacctgccg ggtcatgtcg
      721 atgtcggggc gcgagggcgt cgggtcgccc gaggggtggt tgtgcacgat gatcatggac
      781 ttggccgaca gttccagcgc ccgccgcacg acctcgcgcg gatagacggg agcatggtcg
      841 accgtgccgc gattctggat ttcgtcgagg atcagctggt tgcggttgtc gagatacagg
      901 acccggaact gctcgcgcgg ttcgtgctgc aggctcatgc ggacataggc gaccagggcc
      961 gtccatgatg agatgacggg gcgggcggcg gcttcctcgc gggtgacgcg gcgcgcgacc
     1021 tcgtgcaggg cggccagatc gagagcggtt tcggcgccga cgcctttcga gcgtcccttc
     1081 gcgtcctcgg cgcggacggt catcagatcc tccaccgagg cggccagcac cgccgccagc
     1141 gagccgaagc gggccagcaa agccttggcg acgggtttca cgtcgccctg cggctggctg
     1201 cggaacagga agagttccag caactcgtag tcgggcaggt ggtggaggcc ggcgccgcgc
     1261 gcgcgttcgc gcagccgttc gcggtgaccg gcatggtggg gcgttttcgg ttggtcgagc
     1321 atgcgcctcc ctcgggaggc tacagcctat cctagaattt cgggcggaac agcccggcgg
     1381 ggctggcggt gaaaatctcc agcccgtctt cggtcacgcc gatggtgtgt tcgcactggg
     1441 aggacagcga cttgtcacgg gtcacggcgg tccagccgtc ggacaggacc ttcacgtggg
     1501 gcttgcccag gttcaccatc ggttcgacgg tgaagaacat gccggtcttc agaaccgcgc
     1561 cctcgccgcg gcggccatag tgcaggacgt tggggctgtc gtggaacagc cggccgatgc
     1621 cgtggccgca gaagtcgcgg accaccgaca tgcggttggc ctcgaccacc tgctggatgg
     1681 cgtagccaat atcgccgaac gtattgcccg gcttgatcac ggccagaccg gcgtcgagcg
     1741 agtcataggt gacgtcgatc agcttcttcg cccgcgcgtt gatgtttccg accgcataca
     1801 tgcggctgga atcaccgtgc caaccgtcga cgatgacggt gtgatcgatg ttgacgatgt
     1861 cgccgtcctt gagaaccttg tcgccgggga tgccgtggca gacgacgtgg ttgatcgagg
     1921 tgcagatcgt cttctcatag cccttgtagc cgaggcaggc gggcagggcg ccgtggtcga
     1981 gggtgaactc gcggatgcgg tcgtcgagct cgccggtggt cacgcccggg accacgaacg
     2041 gggtgatcat gtcgagcgcc tcggccacca gacggccggc cctgcgcatg ccctcgaaag
     2101 cctccgcgtc atggacgcga atctcgtgag tgcggacgaa ggcgtcgtcg tccagttccg
     2161 gggtctcgta cattcgatcg gctttcgggg tcggcttggc tctcaagtgg gagagtatag
     2221 cacaaacctc aaggccgggt tgaggccgcc gggcgtcccg cgaacgcctg cgtcgccgcg
     2281 gcataggccg ggttcgcccg gcccttcgcg tcgagcagca gggggctgtc atcggtccag
     2341 cccttgcctt cggggcggag cagccagctg tcggtgtcgc gcaggcccca ggtcgtgaag
     2401 gcgtaacgct ggcgttccgg cagggccatg aaggcctcgg ccagctcgcc gacgcgggcg
     2461 acctgctggg cgcgtttcgc ggcgagggag cgggtgtcga ggcggttgtc ggagcgcatg
     2521 gagaaatcca gctcggacac atggatgggc aggcccaggg agccggcgtc cttgatgaag
     2581 gtcctgatct ggccggcggg gatttcgata tcgaggtggg actgggtccc gatgccgccg
     2641 atgggggcgc cgagggtgag cagccgctcg accaaccgca ggaaggtcgc gcccttacgc
     2701 gggatgttct cgagattgta ttcgttgagg aacaggacgg cgtcggggtc ggctgcgcgg
     2761 gcctgttcga aggcgcggac gatgtagcct tcctgtccga gggcccggct gaaatggcag
     2821 tcgcgcaggc cgtcgccgtc ttcagccacc ggctcgttga cgacgtccca gccggccatc
     2881 ctgccgcgat agcgtccggc gacggtggag acataacggt cgtactcggc gccgaaccgg
     2941 gcgcggggca ggacgtcggt gaaggcatcc tggccctggg agtaccagac gagggtgtgg
     3001 ccgtggaccc gcatcccctg ttcgcgaccc cagtcgacga tgcggtccga ggcggagaag
     3061 tcgtatgtaa agtccggctg caggatgcgt tcgggcttca gctcccattc gggggtcagc
     3121 tggctgacgt gcttgcgcat ctgcgcgacc cagacggggt cggacagctg gccggtcatg
     3181 ccggttgcgc cgatggggaa ggaggcgagg tccttcagcg acgcggcctc ggggcccggc
     3241 gtcccggccg aggcgaagcg attgcaggcc gcgagggcga agggcgcagc gaggagggag
     3301 cggcggtcga tcatgtccct gcctccgagg cgcgggtcag cccgcgcggc ggcgcagacc
     3361 cagttcgtcg aaggcggcgg tttcgcgctt gccggcggcg atgatgctgt tcagccgggt
     3421 ggtctcggcg acgtgttcga acttcttcag ttcctcgaag gcgccggtca gggcgtcgcg
     3481 ggcgccttcc tcctcggcgc tgatgacgac cacatcggcc tcggccgcgc ccttgcgcag
     3541 tttccagccg ctcagatagg cctgaagcat catggcggcg tcggcgtcgg aacgggcgcg
     3601 ttcctgttcg atttcggcct cggcctccag aacggcgatg cgcatctcgg cggaggcgcg
     3661 gcgcgccgtg atctcggcca gacgcttctg cagcgtctcg acctcgtagt tggagatccg
     3721 gatcagcgat tgggcccagg cggtcatctg cgtactccgg tcactggatc attccctgat
     3781 tgaggattgc ttccagctgg gtaaaggaat cggaaagaga cgtgtgatcg tccttgtcct
     3841 gaccgaggaa attctcgagc gcggggttca gggcgatggc gcggtcgacg atcgggtcgg
     3901 ccccggtgcg gtaggcgccg atgcggatca gctcttccat gttggcgtag gcgctgaggc
     3961 actggcgggc gcgcttgttc agttcgcgtt cgggcggcgt ctggcaggcg ggcagggtgc
     4021 ggctgacgga cttgagcacg tcgatggccg ggaagcggcc gcgctcggcg atcttgcggt
     4081 ccatgacaat gtggccgtcg aggatacctc gcacggcgtc ggcgatgggc tcgtcatggt
     4141 cgccgccgtc gaccaggacg gtgaacaggg cggtgatcgg cccagcggtg gtgccgtcgg
     4201 gacggacggg accgggtcct gcgcgctcca gcagcttggg cagttcggtg aagacggtgg
     4261 gggtgtagcc cttggtggtc ggcggctcgc cggcggcgag gccgatctcg cgctgggcca
     4321 tggcgaagcg ggtgaccgag tccatcaggc acaggacctc cagatcctga tcgcgcaggt
     4381 attcggagat ggccatggtc atataagcgg cctgccggcg cttcagcgcc ggctcgtccg
     4441 aggtggcgac gacgacgatg gcgcggcgca ggccttcctc gccgagtgtt tcctcgatga
     4501 attcgcggac ctcacggccc cgttcgccga tcaggccgac cacgacgacg tcacaggtcg
     4561 cctgacgcgc cagcatggac agcagtaccg acttgccgac acccgagccg gcgaagatgc
     4621 cgaggcgctg gccccggcag gtggtggtga agacatccat cgaccggacg ccgaggtcga
     4681 ggcgctctcc gacccgcccg cgcgaatggg ccgggggcgg gggcgcgcgc aggggatagg
     4741 cggcggggcc ctgcggcaga ggacccttgc cgtcgatggg ctggccgaag gcgtcgatga
     4801 tccggcccag ccaggcggtg gtcggtcgta cgctggcggc ggcggagacg atgcgaatgt
     4861 cggcgccggg ggccacgccc tcgaccggac cgaaaggcat cagcagggcc ttggcgtcgc
     4921 ggaagccgac gacctcggcc ggcaggggat gcagatggcg gcgctcgatc tcggctcgtg
     4981 cgccgacagc gaggcgggac aggccgccgc gggattcgat cagcaggccg ttcacgcccg
     5041 cgaccttgcc ggtcacgaca agcggatcga cggcttcgac ggcggcggcg agattacgca
     5101 aggctggggt cccaggatca gtccctcacg gatgcgctga cgtggttaac gggggatgaa
     5161 ggccgttcga ccgacccaca atctgccggt tgcttgcggc ggcgagggca aggtttcgac
     5221 ggggtccctc tttgcttggc cgccggaacg gttatctctt gagccgcaac ttccggagac
     5281 ctctcatgtc cgacgccccc tatgatccct cccgccatct gaaggccggg cgcggcaagg
     5341 tctatggctc
//
LOCUS       DASVVN010000660         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_341639,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000660 DASVVN010000000
VERSION     DASVVN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341639"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VF686_10830"
     CDS             complement(<1..700)
                     /locus_tag="VF686_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX8662349.1"
                     /translation="MTPNLDLAPVGNCSISALIDRNGRYVWACAPRVDGDPVFSALMD
                     GFDPDHGFWDIELEGQATVEQAYIRNTPVLRTVLTAEDGASVEIIDFAPRHPKHSRTY
                     RPLAFGRIVRPLSGSPRIRVRLRPSADWGARRAECTSGSNHIRYLCTDVTFRLTTDCP
                     VSHILNERTFRLERSHAFFLGPDEGYDQDVGPGVSAALDRTIAYWQDWVRTLYLPLDW
                     QEAVIRAAITLKLCA"
     gene            complement(697..1434)
                     /gene="otsB"
                     /locus_tag="VF686_10835"
     CDS             complement(697..1434)
                     /gene="otsB"
                     /locus_tag="VF686_10835"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX8662350.1"
                     /translation="MPHLQSPPARLPRPALFLDLDGVLAPLAPTPDAVIPDPRRTAVL
                     RSLAERLGGRVAIVSGRTIAEIDRIAETALTPASGVHGLERRRADGSLTRATADPAVR
                     DVIAAFTRFAETRPGTVVEDKAVSAGLHFRGAPAEAGAAGALAERLAGETGLTLQAGH
                     LVIELKTPGADKGTALTDFMREPPFAGAVPVMLGDDLTDEHGFEAAEALGGFGVLVGP
                     PRETAARYGLPDVDAVLAWLEAVEETA"
     gene            1604..2596
                     /locus_tag="VF686_10840"
     CDS             1604..2596
                     /locus_tag="VF686_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX8662351.1"
                     /translation="MTDTAKPLAADPAAMTARAREVIQLNIAALQALETSLDDGIARA
                     CEIILSRPGYLVVTGMGKSGHIGGKIAATLASTGTNAFFVHPAEMSHGDLGMLRKDVT
                     LLAISNSGESRELRDPLIYCQREGIPVIGLTQRPNSFLGRSSAVCLTMPQVAEACPNG
                     LAPTTSTLMTLALGDALAMVLMDRRGFTAEAFGLHHPGGKLGMSLQSVRDWMGDRPPA
                     PATVPLTATFAEVVSAITEGRKGAAAVLADDGTLAGIVTDGDIRRAFSRDLNGLTAAD
                     VMSAKPKVIGPDARMSDAVDLMTQNKIATLLVVEDHRPIAIVHIAELMQAGYVG"
     gene            complement(2605..3201)
                     /locus_tag="VF686_10845"
     CDS             complement(2605..3201)
                     /locus_tag="VF686_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8662352.1"
                     /translation="MRRSLFLILASLLPVSACADAYPPQAAPIPRIEPASQSAREVVP
                     IAPPRTSRPSDQIETRLLQAHNGERRRVGSPPLAWSDELEAEARGWAQELISTGRFAH
                     DPSMHGHGENLWMGWGNRAFTPEDMVGDWIEEKAQYRHGVFPHVSRTPNWTDVGHYTQ
                     LVWRSTTHVGCAIVTRDDRSILACRYAPPGNIDGRRAY"
     gene            complement(3267..>3733)
                     /locus_tag="VF686_10850"
     CDS             complement(3267..>3733)
                     /locus_tag="VF686_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8662353.1"
                     /translation="KATSKSKKQALAEQACLELKVHTVIEEEVFYPACRGKIEEDLLN
                     EAYVEHDSAKLLINEIEAGGADEEFYDAKVKVLSEMIEHHVEEEEKRSEGMFSQARAA
                     GLDMDALADAMRARKMTAMEEFQAGKPAETLTLEGDDTEKALMDTPMGGAAR"
BASE COUNT          544 a         1315 c         1265 g          609 t
ORIGIN      
        1 aggcgcagag cttcagggtg atcgccgcgc gaatcaccgc ctcctgccag tcgagcggca
       61 gataaagcgt gcgcacccag tcctgccaat aggcgatggt gcggtccagg gccgccgaaa
      121 ccccgggacc gacgtcctga tcgtagccct cgtccggacc gaggaagaag gcgtgggacc
      181 gttccagccg gaaggtccgc tcattgagga tgtgggacac cggacagtcg gtggtcagcc
      241 ggaaggtcac gtccgtgcac agatagcgga tgtggttgga cccgctggtg cattcggccc
      301 ggcgcgcgcc ccagtcggcg gacggacgca gacgcacgcg aatccgcggc gacccgctca
      361 gcggccggac gatccgtccg aaggcgagcg gccggtaggt gcggctgtgt ttcggatgcc
      421 ggggcgcgaa gtcgatgatc tcgaccgacg ccccgtcctc cgctgtcagc accgtgcgca
      481 ggacgggggt gttgcggata taggcctgct cgaccgtcgc ctgcccttcc agctcgatgt
      541 cccagaagcc gtggtccggg tcgaagccgt ccatcagggc cgagaacacc gggtctccgt
      601 cgacgcgcgg cgcgcaggcc cagacatagc gcccattgcg gtcgatcagg gcgctgatcg
      661 agcagttgcc gacgggtgcg agatcgaggt tcggggtcat gcggtctcct cgacggcctc
      721 cagccaggcg aggacggcgt ccacgtccgg gagtccatag cgggcggcgg tctccctcgg
      781 cggcccgacc agcacgccga acccgcccag ggcctcggcg gcctcgaacc cgtgctcgtc
      841 cgtcagatcg tcgcccagca tgaccggaac ggcgcccgcg aacggcggct cccgcatgaa
      901 gtcggtcagc gccgtgccct tgtcggcgcc gggcgtcttg agctcgatca caaggtgacc
      961 ggcctgcagg gtcaggcccg tttcaccggc gagccgttcg gccaaggccc cggcggcgcc
     1021 cgcctctgcg ggggctcccc ggaaatgcag gccggcggag accgccttgt cctcgacgac
     1081 ggttcccggg cgggtttcgg cgaaccgcgt aaaggcggca atgacgtcgc gcaccgccgg
     1141 atcggccgtc gcccgggtca gggagccgtc ggcccggcgc cgctccagcc cgtgcacgcc
     1201 cgaggccggg gtcagggcgg tttcggcgat ccggtcgatc tcggcgatcg tccggccgct
     1261 tacgatggcg acgcgcccgc ccagccgttc ggccagcgat ctgagtacgg ccgtgcgccg
     1321 tgggtccgga atgaccgcgt ccggggtggg ggccagcggc gccagcacgc cgtccagatc
     1381 gaggaacagc gcgggccgcg gcaggcgcgc gggcgggctt tgaaggtgcg gcatggcttc
     1441 ggcggccgtt tgcgcggctt tgctctgttg cgacgtcccc atggctgcca aacgcgcagg
     1501 ggcccggttg gttgtctcaa ggcgaagctt ttcttcgcat tgcagcattc gctgtgggtc
     1561 cgccgccgtt gtgaaacgcg cgtggatgag ccaattcctg cgcatgaccg acaccgccaa
     1621 gccccttgcc gcagaccccg ccgccatgac cgcccgcgcc cgcgaggtga tccagctaaa
     1681 catcgctgcg ctgcaggcgc ttgagaccag cttggacgac ggcatcgccc gcgcctgcga
     1741 gatcatcctg tcgcggcccg gctatctggt ggtgaccggc atgggcaagt ccggccacat
     1801 cggcggcaag atcgccgcga ccctggcctc caccggcacc aacgccttct tcgttcaccc
     1861 ggccgagatg agccacggcg acctcggcat gctccggaag gatgttacgt tgctggccat
     1921 ctccaactcg ggcgaaagtc gcgagttgcg tgatccgctg atctattgcc agcgcgaggg
     1981 cattcccgtc atcggcctga cccagcggcc caacagcttc ctcggccgct cgtccgccgt
     2041 ctgcctgacc atgccgcagg tggccgaggc ctgccccaac ggcctggccc ccacgacctc
     2101 gaccctgatg accctggcct tgggcgacgc cctcgccatg gttctgatgg accggcgcgg
     2161 cttcaccgcc gaggccttcg gcctgcatca ccccggcggc aagctgggca tgagcctgca
     2221 gagcgtgcgc gactggatgg gcgaccgtcc gccggccccg gcgaccgtgc ccctgaccgc
     2281 gaccttcgcc gaggtcgtct cggccatcac cgagggccgc aagggcgcgg cggccgtgct
     2341 ggcggatgac gggacgctgg ccgggatcgt caccgacggc gacatccgcc gggccttctc
     2401 gcgcgacctg aacggcctca ccgccgccga cgtcatgagc gcgaaaccca aggtcatcgg
     2461 ccccgacgcc cgcatgagcg acgccgtcga cctgatgacc cagaacaaaa tcgccaccct
     2521 gctggtggtt gaggaccacc ggcccatcgc catcgttcac atcgccgaac tgatgcaggc
     2581 cggctacgtc gggtaagtcc cgggctagta agcgcggcgt ccgtcgatat tgcccggcgg
     2641 cgcatagcgg caggccagta tcgagcggtc gtcccgtgtg acgatggcgc agcccacgtg
     2701 ggtcgtgctg cgccagacca gctgggtgta gtgaccgacg tcggtccagt tcggcgttcg
     2761 gctgacgtgc gggaacacgc cgtgacggta ctgggccttc tcctcgatcc agtcgccgac
     2821 catgtcctcc ggcgtgaagg cgcggttgcc ccagcccatc cacaggttct cgccatggcc
     2881 gtgcatggag ggatcatggg cgaagcggcc ggtggagatc agttcctggg cccagcccct
     2941 cgcctcggcc tccaactcgt cgctccatgc cagtggcggc gagccgacgc ggcgtctctc
     3001 gccattgtga gcctgaagca ggcgcgtctc gatctggtcc gagggccgcg aggtgcgcgg
     3061 cggggcgata ggcaccacct cccgtgcgga ctgcgacgcc ggttcgatcc tcgggatcgg
     3121 cgcagcctgc ggcgggtagg cgtccgcgca ggccgagacc ggcagcaggc tcgccagaat
     3181 cagaaacagg gaacggcgca tcgggtctcc tcaatcaggt ggggacatag cgtcccggcc
     3241 ccctgcgccg ttccttgtct ccgtcttcag cgtgcggcgc cgcccatggg cgtgtccatc
     3301 agggcctttt cggtgtcgtc gccttccagc gtcagggttt ccgcaggctt gccggcctgg
     3361 aactcctcca tcgcggtcat cttgcgggcc cgcatggcgt ccgcgagggc gtccatgtcg
     3421 aggccggcgg cgcgcgcctg cgagaacatg ccctccgagc gcttctcctc ctcctcaacg
     3481 tggtgctcga tcatctccga caggaccttg accttggcgt cgtagaattc ctcgtcggcg
     3541 ccgccggcct cgatctcgtt gatcagcagc ttggcgctgt cgtgctccac ataggcctcg
     3601 ttcagcaggt cttcctcgat cttgccgcgg caggccggat agaagacctc ctcctcgatg
     3661 acggtgtgaa ctttcagttc cagacaggcc tgttcggcca gcgcctgctt cttcgatttc
     3721 gaagtcgcct tct
//
LOCUS       DASVVN010000661         6886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_446340,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000661 DASVVN010000000
VERSION     DASVVN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446340"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VF686_10855"
     CDS             complement(<1..958)
                     /locus_tag="VF686_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX8662354.1"
                     /translation="MSIDSLTSAAAITGAEPWLVLPPALAIPPGSGAICDADGPRKIG
                     RGAAEGAFTTAPVMVAHASLTARRLGIAPPPRSADILDVLELFAFVRPARFCAPSPSG
                     LALAMGMSEPKGAEAQAQALMDAAAGLLRELADPAYPGREDAFTLAETLGRAGWSWAW
                     RVSGALQHHPVRERQHRGSGLDAWSRLPEWEDEAPRGEAGSAPVDSESARIRLEKLLQ
                     ASGLDETRPTQSDYAAEAAFAFSPRNEEGRPRFLLAEAGTGTGKTLGYLAPASLWAER
                     NQGAAWVSTYTRALQRQIDRESSALWPDPAERKKKTVVRKGRE"
     gene            1017..3677
                     /locus_tag="VF686_10860"
     CDS             1017..3677
                     /locus_tag="VF686_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX8662355.1"
                     /translation="MTPPPAPATLSPPFADWFARRGWSPRRHQLEMIAAADAGSHALL
                     VAPTGGGKTLAGFLPSLVELAERGPRPAFGPGSGIHTLYLSPLKALTTDVERNLMTPI
                     REIGLNIHVESRTGDTNQSKKARQREFPPDILLTTPEQLALFCAWDGARAYFADLKCV
                     VLDEVHAIWSGKRGDLLSLGLGRLQQFAPGMRRVALSATVDDPEMIADWLRPGAVVYP
                     STPSGSPSPFHGEGTARTVSSLSRSDGEGDPEGVEGLPSARDVVIVRGDPGAPPVVDV
                     LVSEGKVPWAGHTGQHAIPEVYEAIRRSGMSLIFVNTRWQAEFVFQQLWSINDDNLPI
                     GLHHGSLAAEQRRKVEAAMARGDLRAVVCTSTLDLGIDWGDVDLVIQLAAPKGASRLV
                     QRIGRANHRLDEPSRALMVPASRFEMLECQAAREAVAENAFDWEPVHTGTLDTLAQHV
                     MGCACSEPFLMDDLYTEVTGCGPYRGLTYEQFEEVVDLVATGGYALRTYERFARIVRT
                     RDGRWKVRNAVTAQRHRMNVGAITAAATLNVRIASRRGGAAKQLIGGRKVGEAEESYF
                     EPMTPGDTFVFAGQVWAFQGINGMDALVTHAQDSDPKIPSWGGSKFPMSTSLADRVRA
                     MIQDRDHWRVLPPDVQEWLELQEERSVIPDAQTMLVETFPRGSRHFLVAYPFEGGLAH
                     GTLCMLLTRRLDRMGIGPLGFVVTDYSLAIWSIRPMDGLDLDRLFEPDMLGDDLESWL
                     EESFMMKRTFRNCALISGLIERRQPGNEKTGRQVTFSTDLIYDVLRRHQPDHLLLKTA
                     RADAAAGLLDVARLGQMLARIQGQIRHVKLERPSPFCVPVLVQIGRERVGGGQAAEMI
                     LDASAYGFDEADLIAEVMADVPPDFLGAAQ"
     gene            3674..4405
                     /gene="pdeM"
                     /locus_tag="VF686_10865"
     CDS             3674..4405
                     /gene="pdeM"
                     /locus_tag="VF686_10865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX8662356.1"
                     /translation="MNAALRQALSPARCPSGGLRVHIEGEPCVLRCSGALWVLNHRTL
                     IAADLHLEKGSAFAARGQMLPPYDSRATLDRLEAEIDALQPAAVVLLGDSFHDAKSVG
                     RMATDDKARLDRLAAGRDWIWLEGNHDIAALAGSMDHLVGEVVETMELGSLRLIHEPQ
                     AGVRPGEIAGHLHPAARVAAYGRGVRRPCFVTDGRRAVLPAFGAFTGGLDVRDPVIAG
                     LFGEPPMAAALGRDRVHALAWETLR"
     gene            complement(4402..4758)
                     /locus_tag="VF686_10870"
     CDS             complement(4402..4758)
                     /locus_tag="VF686_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662357.1"
                     /translation="MITAIVGLLLAAGPFGQEQSQPEPVSLKCERGPVHRVFGGTPWL
                     VYGCDDGASIVIVSGPGSPAAPFYFLMAFKDGAYWITGEGNGNQAATTAAFEELKLLD
                     RSAITALAEEARGASD"
     gene            complement(4755..5489)
                     /locus_tag="VF686_10875"
     CDS             complement(4755..5489)
                     /locus_tag="VF686_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8662358.1"
                     /translation="MTTIRSLLFVAWLYLSMALFAVGLSPALLMPYRQSMWVIRSWSK
                     FCLFGLRWIAGVKVEFRGLEHRPTGAALIAGKHQSMLDVIAPFAVLPDNCFIMKQELA
                     PLPFFGWFATKTRMIAVDRSAHAKALKDMVRQARDRYAEGRQILIFPEGTRAPVGAPA
                     DYKPGIAALYRDLEADCTPMATNSGVHWPAHGFRRHPGTVVYEFLPAIPAGLKRAEFM
                     TVLEERLETASNALLTGPSKQIGQGS"
     gene            complement(5491..6132)
                     /locus_tag="VF686_10880"
     CDS             complement(5491..6132)
                     /locus_tag="VF686_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX8662359.1"
                     /translation="MKFLTFIAILALIWAIGLFAFADRVRGFTPAPEPERADAIVALT
                     GPSAERVNAAIRLLEQDKGERVLISGVNREVRRQELRALTPGSNKLFNCCVDLGFEAE
                     DTLGNAQEIAAWADAKDYDTLIVVTSDYHMPRALTEIRAAAPGVRLTPYAVETPSLDD
                     SRWWKAAVTARRMTLEYMKYLAALGRAMVGGGRASADAVPVDAPVNASGEKAA"
     gene            complement(6142..>6886)
                     /locus_tag="VF686_10885"
     CDS             complement(6142..>6886)
                     /locus_tag="VF686_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8662360.1"
                     /translation="AAHGWARQLRSEATVQVRPRVGETGAEAAARAAETLAGVPGVTE
                     AEAMDRKTAEALLRPWLGDAVLPDLPIPHLVTVRLDRTAPASAVSLSRALSSAGLDAT
                     VDDHSLWRGEVERSAALITVLALAAFLLTAGAAGAAVAYATRAGMAAQTGVIDTLSLN
                     GASDSAIAGLYQVRYGRVAAVAGGIGALTAMILVAGLRFLGGAEGVTPALPLMWRDVL
                     ILSPCPLLAATVALAAARFTALARLKGRH"
BASE COUNT         1030 a         2229 c         2606 g         1021 t
ORIGIN      
        1 tctcgcgccc cttgcggacg acggtcttct ttttccgctc ggcgggatcg ggccagagag
       61 cagagctctc gcggtcgatc tggcgctgca gggcgcgggt gtaggtcgag acccaggcgg
      121 cgccctgatt gcgttcggcc cacaggctgg cgggggcgag atagccgagg gtcttgccag
      181 tgcccgtgcc ggcctcggcc agcaggaaac ggggacggcc ctcctcgttg cgcggcgaga
      241 aggcgaaggc ggcctcggcg gcgtagtcgg actgggtcgg acgggtctcg tcgaggcccg
      301 aggcctgcag cagtttctcg aggcgaatgc gggcggactc gctgtcgacc ggggcggaac
      361 cggcctcgcc gcgcggggcc tcgtcctccc actccggcag gcgggaccaa gcgtcgagac
      421 cgctgccgcg atgctggcgt tcgcggaccg gatggtgctg caaggccccc gagacgcgcc
      481 acgcccacga ccagccggcg cggccgaggg tctcggcgag ggtgaaggcg tcctcgcgcc
      541 ccggataggc ggggtcggcc agttccctga gcaggccggc ggcggcgtcc atcagggcct
      601 gcgcctgcgc ctcggcgccc ttgggctcgc tcatccccat ggccagcgcc agaccgctgg
      661 gcgagggggc gcagaagcgg gcggggcgga cgaaggcgaa cagctccagc acgtcaagga
      721 tgtccgccga gcgcggcggc ggggcgatgc cgaggcggcg tgcggtcagg ctggcgtggg
      781 cgaccatgac gggggcggtg gtgaaggccc cctcggccgc gccgcgcccg atcttcctcg
      841 gcccgtcggc gtcgcagatt gcgccggacc cgggcgggat ggcgagcgcg ggcggcagga
      901 ccagccacgg ttcggcgccg gtgatggccg ctgcggatgt gagggagtcg atggacacag
      961 ccgatagata gcggttccgg aggtgaaacg gaacgggaac atgctatagg cgcgatgtga
     1021 cgccgccgcc cgctcctgca acgctttcgc cgcccttcgc cgactggttc gcgcggcgcg
     1081 gctggtcgcc gagacggcac cagctggaga tgatcgcggc cgcggacgcc ggctcgcacg
     1141 ccctgctcgt cgcgccgacc gggggcggca agacgctggc ggggtttctg ccgtcgctgg
     1201 tcgagctggc cgagcggggg ccgaggccgg cgttcggacc gggcagcggg atccacaccc
     1261 tgtatctgtc gccgctgaag gcgctgacca ctgacgtcga gcgcaacctg atgacgccga
     1321 tccgcgagat cgggctgaac atccatgtcg aaagccgcac cggggatacg aaccagtcga
     1381 agaaggcgcg gcagcgcgag tttccgcccg acatcctgct gaccacgccg gagcagctgg
     1441 ccctgttctg cgcctgggac ggggcgaggg cttatttcgc cgacctgaaa tgcgtggtgc
     1501 tggacgaggt gcacgcgatc tggagcggca agcgggggga cctgctgtcg ctggggttgg
     1561 gtcggctgca gcagttcgcg ccggggatgc gacgggtggc cctgtcggcg acggtggatg
     1621 atccggagat gatcgcggac tggctgcggc cgggtgctgt cgtttacccc tccacccctt
     1681 cggggtcccc ctccccattt catggggagg ggacggcgcg gactgtctcc tccctgtcgc
     1741 gtagcgatgg ggagggggac cccgaagggg tggaggggtt gccgagcgcg cgggatgtcg
     1801 tgatcgtccg cggcgacccg ggggcgccgc cggtggtgga tgtgctggtc agcgagggta
     1861 aggtgccgtg ggcggggcac accggccagc acgccatccc cgaggtctat gaggcgatca
     1921 gacggtcggg gatgagcctg atcttcgtca acacgcgctg gcaggcggag ttcgtgttcc
     1981 agcagctgtg gtcgatcaat gacgacaatc tgccgatcgg cctgcaccac ggctcgctgg
     2041 cggcggagca gaggcgcaag gttgaggcgg cgatggcgcg gggcgatctg cgcgcggtgg
     2101 tctgcacctc gacgctggat ctgggtatcg actggggcga cgtcgatctg gttatccagc
     2161 tggcggcgcc caagggggcc tcgcggctcg tgcagcggat cggccgggcc aaccaccggc
     2221 tggacgagcc gtcgcgcgcc ctgatggtgc cggccagccg gttcgaaatg ctggaatgcc
     2281 aggccgcgcg cgaggcggtg gccgagaacg ccttcgactg ggagccggtg catacgggaa
     2341 cgctggatac gctggcccag cacgtcatgg gctgcgcctg ttccgagccg tttctgatgg
     2401 atgatctgta cacggaagtt accggctgcg gtccctatcg cggcctgacg tacgaacaat
     2461 tcgaggaggt ggtcgatctg gtcgccaccg gcggctacgc cctgcgcacc tatgagcggt
     2521 tcgcgcggat cgtgcggacg cgggacggcc ggtggaaggt gcgcaacgcc gtgacggcgc
     2581 agcggcaccg gatgaacgtc ggcgccatca ccgccgccgc caccctcaac gtccgcatcg
     2641 cctcccgtcg tggcggggcg gcgaaacagc tgatcggcgg ccgcaaggtc ggcgaggccg
     2701 aggagagcta tttcgagccg atgacgcccg gcgacacctt cgtcttcgcc ggtcaggtct
     2761 gggccttcca gggcatcaac ggcatggacg cgctggtcac ccacgcgcag gacagcgatc
     2821 cgaagatccc gtcgtggggc ggctccaaat tccccatgtc gacctcgctg gccgaccggg
     2881 tccgggccat gatccaggat cgcgaccact ggcgcgtcct gccgcccgac gtgcaggaat
     2941 ggctggagtt gcaggaggag cggtccgtca ttcccgacgc gcagaccatg ctggtcgaga
     3001 cgtttccgag gggcagccgc catttcctcg tcgcctatcc tttcgagggc gggcttgcgc
     3061 acgggacgct gtgcatgctg ctgacgcggc ggctggaccg gatggggatc gggccgctgg
     3121 gcttcgtggt caccgactat tcgctggcca tctggtcgat ccggccgatg gacgggctgg
     3181 acctcgaccg gctgttcgag cccgacatgc tgggggacga tctggagagc tggctggagg
     3241 agagctttat gatgaagcgc accttccgca attgcgccct gatctcgggc ttgatcgagc
     3301 ggcgccagcc ggggaacgag aagaccggac ggcaggtgac cttctccacg gacctgatct
     3361 acgacgtgct gcgccgccat caacccgatc atctgctgct caagaccgcc cgcgccgatg
     3421 cggcggcggg tctgctggac gtggcgcggc tgggccagat gctggcgcgg atccagggac
     3481 agatccggca tgtaaagctg gagcgaccct cccccttctg cgtgccggtg ctggtgcaga
     3541 tcgggcgcga gcgggtcggc ggcggacagg cggcggagat gatcctcgac gcctcggcct
     3601 atggcttcga cgaggccgat ctgatcgcgg aagtcatggc cgacgtgccg cctgatttcc
     3661 tcggggcggc gcaatgaacg cggcgctcag acaggccctg tcgccggcgc ggtgccccag
     3721 cggcgggctg cgcgtccata tcgagggcga gccctgcgtg ctgcgctgtt cgggcgcgct
     3781 gtgggtgctg aaccatcgca ccctgatcgc ggccgacctg catctggaga agggctcggc
     3841 cttcgccgcg cgcggccaga tgctgccgcc ctacgacagc cgggcgacgc tggaccggtt
     3901 ggaagccgag atcgacgccc tgcagccggc ggccgtggtc ttgctgggcg acagcttcca
     3961 cgacgcgaaa tcggtcggcc ggatggcgac ggacgacaag gcgcgtctgg acaggctggc
     4021 ggcgggccgc gactggatct ggctggaagg caaccacgac atcgccgcgc tggcgggctc
     4081 gatggaccat ctggtcggcg aggtggtcga gacgatggaa ctcggctcgc tgcgcctgat
     4141 ccacgagccg caggcaggtg tgcgtccggg cgagatcgcg ggccacctgc atccggcggc
     4201 gcgggtggcg gcctatgggc gcggggtgcg gcggccgtgt ttcgtgacgg acgggcggcg
     4261 ggcggtgctg ccggcgttcg gagccttcac cggcgggctg gatgtgaggg acccggtgat
     4321 cgcgggactg ttcggggaac cgccgatggc tgcggcgctc gggcgggacc gggtgcacgc
     4381 gctggcgtgg gagacgctgc gctagtcgga agccccgcgg gcttcttcgg ccagtgcagt
     4441 gatggctgac cgatcgagca gcttcagttc ttcaaaggcc gcagtcgtcg ccgcctgatt
     4501 gccgttgcct tctcccgtaa tccagtaggc gccgtccttg aaagccatca ggaagtagaa
     4561 gggagccgcc gggctgccgg ggcctgagac gatgacaatg ctcgctccgt cgtcgcagcc
     4621 gtagaccagc catggcgtac ccccgaagac cctgtgaacg ggtccccttt cacacttcag
     4681 ggaaacgggt tcaggctggc tctgctcctg accaaacgga ccggcagcaa gcagaagccc
     4741 aacgattgcg gtgatcacga tccctgcccg atctgcttgc tcggccctgt aagcagcgcg
     4801 ttcgacgccg tctccagccg ctcttccagc accgtcatga actccgcccg cttcagcccc
     4861 gccggaatcg ccggcaggaa ctcatagacc accgtccccg gatgccgccg gaagccgtgc
     4921 gccggccagt ggacgccgga gttggtggcc atgggggtgc agtcggcctc gaggtcgcgg
     4981 tagagggcgg cgatgccggg cttgtagtcg gcgggggcgc cgacgggcgc gcgggtgcct
     5041 tcggggaaga tgaggatctg gcggccctcg gcgtagcggt cgcgggcctg acggaccatg
     5101 tccttgagcg ccttggcatg ggccgagcgg tcgacggcga tcatccgggt cttggtggcg
     5161 aaccagccga agaagggcag gggggccagc tcctgcttca tgatgaaaca gttgtcgggc
     5221 aggacggcga agggggcgat cacgtccagc atcgactggt gcttgccggc gatcagggcg
     5281 gcgccggtcg ggcggtgctc gaggccgcgg aactcgacct tgacccccgc aatccagcgc
     5341 agcccgaaca ggcagaattt cgaccagctg cggatcaccc acatcgactg gcggtagggc
     5401 atcagcaggg ccggcgacag gccgacggcg aacagggcca tcgacagata gagccaggcg
     5461 acgaacagca gcgaccggat cgtggtcacg tcaggcggcc ttttcgccag aagcgttaac
     5521 gggggcgtca acggggacgg cgtctgctga cgcccggccg ccgccgacca tggcccggcc
     5581 aagggctgcg agatatttca tgtattcgag cgtcatccgc cgggcggtaa ccgcggcctt
     5641 ccaccagcgc gaatcgtcca gcgacggcgt ctcgacggcg tagggggtga ggcgcacgcc
     5701 cggggcggcg gcgcggatct cggtcagggc ccggggcatg tgatagtcgg aggtcacgac
     5761 gatcagggtg tcgtagtcct tggcgtcggc ccaggcggcg atctcctggg cgttgcccag
     5821 ggtgtcctcg gcctcgaagc ccagatccac gcagcagttg aacagcttgt tcgagcccgg
     5881 ggtcagggcg cgcagttcct gacggcggac ctcgcggttg acgccggaaa tcagcacccg
     5941 ctcgcccttg tcctgctcca gcaggcggat ggcggcgttg acccgctcgg ccgacgggcc
     6001 agtcagggcg acgatcgcgt cggcgcgctc gggctcgggc gcaggcgtga agccgcgcac
     6061 ccggtcggcg aaggcgaaca gtccgatcgc ccagatgagc gccagaatcg cgatgaaggt
     6121 cagaaacttc atgcggttaa cctagtgacg ccccttcaat cgcgccagcg ccgtgaagcg
     6181 ggccgccgca agcgccaccg tagcggccag cagcgggcat ggcgagagga tcaggacatc
     6241 gcgccacatc agcggcaggg cgggcgttac gccctcggcg ccgccgagga aacgcagtcc
     6301 cgcgaccaga atcatggccg tcagggcgcc gattccgccc gcgaccgccg ccacccgtcc
     6361 gtaccggacc tgatagaggc cggcgatggc gctgtcagag gcgccattga ggctgagggt
     6421 gtcgatcacc ccggtctggg ccgccatgcc cgcgcgggtg gcgtaggcca cggcggcccc
     6481 cgcggccccg gcggtcagca gaaaggcggc cagcgccaga acggtgatca gggcggccga
     6541 ccgctcgacc tcgccgcgcc aaaggctgtg gtcgtcgacg gtggcgtcga ggccggcgct
     6601 ggagagggcg cggctcaggc tgacggcgct ggcgggggcc gttcggtcca gccgcacggt
     6661 gaccagatgg gggatgggca ggtcgggcag gacggcgtcg cccagccacg gccgcagcag
     6721 ggcctcggcg gtcttgcggt ccatggcctc agcctcggtc acgccgggca cgccggcaag
     6781 cgtttcggcg gcgcgggcgg cggcctcggc ccccgtctcg ccaacgcgcg gccggacctg
     6841 aaccgtggct tccgagcgaa gctggcgcgc ccagccgtgg gcggcg
//
LOCUS       DASVVN010000662         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_208984,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000662 DASVVN010000000
VERSION     DASVVN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208984"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VF686_10890"
     CDS             <1..1648
                     /locus_tag="VF686_10890"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEX8662361.1"
                     /translation="GSWQKDKLTLSGDLGTRHDLALQTFDRLRESLSPAGAVTQTTTQ
                     TQNIDGESDVQFLRLGADYRLSDEIQLTTELRGNLIENGGGGLETYDTRTAAGVPVSF
                     YTRDGNFNFEGDNIGVTGRVLRRFDDQGHDWTTELRVDRNRGHFSLTSAYDFDLPVSA
                     PVYEQQDSRNRLNLIGFTSAYVRPMPNDGKLRAGYELETRDTAFDNSASRGPAPGALV
                     PDPFVTNRFEAQQAVHAVYATWERPFGKLSAQLGLRLEYADIELNQVTTGITSEQSYF
                     RAYPTLHATYQLTDSQSVRGSYSKRIQRPPPFLLNPFTSYQDPLNLRSGNPDLEPQET
                     DSFEVMWQIRAGQTFYQATAYFRDTSGAFTDVVNDLGGGVLLTRPENLGARRDMGVEL
                     VANGRLHSTLRYNASVNVFQQEIDAAGIPGGQDSSATVVTGRGNLNWQPTADDFLQIA
                     GVWQGESLQAQGTREAGGMINLGYRRKLTEKLAFQATVRDLFDNFGDVTTFETPTFRD
                     RTERTFGGRAWYVGLTYAFGSGPRRPQDPQFDFSAAPTPN"
     gene            1706..2443
                     /locus_tag="VF686_10895"
     CDS             1706..2443
                     /locus_tag="VF686_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX8662362.1"
                     /translation="MRLRVVDLETTGGDRTAEIIEVGIVDVVPLGDGEGQGWKAMPPV
                     SRLFRPRGEISFHAMAVHHLTPAHFCDADPHCDEYALREMFGATPADVMVAHAARFER
                     GFIADTAHGGLPWICTVRSAKAVWPQAPGHSNQVLRYWRGLNLDPALANPAHRAGPDA
                     WVTAHLLIDLLKEATVEQMIAWTAERRPADRIPFGKHRGKPWSEPPEDYLRWMAAQAD
                     MDADAVSAARQELERRAGALAKAGDAG"
     gene            2503..>2783
                     /locus_tag="VF686_10900"
     CDS             2503..>2783
                     /locus_tag="VF686_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8662363.1"
                     /translation="MAGVALLGAIALAGLLVAVYAAWLFHDLPDASELADYRPATATR
                     VYAGDGTLIGEFSDERRIYVPYEQVPEPVIQAFLAAEDRNFFNHGGIDVG"
BASE COUNT          454 a          923 c          967 g          439 t
ORIGIN      
        1 cggctcctgg cagaaggaca agctgaccct gtcgggagac ctgggcacgc ggcacgacct
       61 ggcgctgcag accttcgacc ggctgcgcga aagcctgagt cccgccgggg cggtcacgca
      121 gacgaccacc cagacccaga acatcgacgg ggagtcggac gtccagttcc tgcggctcgg
      181 cgccgactac aggctgagcg acgagatcca gctgaccacc gaactgcgcg gcaatctgat
      241 cgagaacggc ggcggcgggc tcgagaccta tgacacgcgc accgcggccg gggttccggt
      301 cagcttctat acgcgcgacg gcaatttcaa cttcgagggc gacaacatcg gggtcaccgg
      361 gcgcgtgctg cgccggttcg acgatcaggg gcacgactgg acgaccgagc tgcgggtcga
      421 ccgcaaccgc ggccatttct cgctgacgtc cgcatacgac ttcgacctgc cagtttcagc
      481 gccggtgtat gagcagcagg acagccgcaa ccggctgaac ctgatcggtt tcaccagcgc
      541 ctatgtccgc ccgatgccga acgacggcaa gctgcgggcc ggctatgagc tggaaacgcg
      601 ggatacggcc ttcgacaact ccgccagccg gggtccggcg ccgggcgccc tggttcccga
      661 ccccttcgtc accaaccgct tcgaagccca gcaggcggtg cacgcggtct atgcgacctg
      721 ggagcgtccg ttcggcaagc tttcggcgca gctggggctg cggctggagt acgccgacat
      781 cgagctgaac caggtgacga cggggatcac cagcgagcaa agctatttcc gcgcctatcc
      841 gaccctgcac gccacctatc agctgaccga tagccagtcg gtccggggca gctacagcaa
      901 gcgcatccag cggccgccgc ccttcctgct gaatccgttc accagctatc aggacccgct
      961 gaacctgcgg tcgggcaatc ccgacctgga gccgcaggag acggattcgt tcgaggtcat
     1021 gtggcagatt cgggcgggcc agaccttcta tcaggcgacc gcctatttcc gcgacacctc
     1081 gggggccttc acggatgtgg tcaacgatct gggcggcggc gtgctgctga cccggcccga
     1141 gaacctcggg gcgcggcgtg acatgggtgt ggagctggtc gccaacgggc ggctgcattc
     1201 gaccctgcgc tacaacgcca gcgtcaacgt gttccagcag gagatcgacg cggccggcat
     1261 tccgggcggg caggacagtt cggctacggt ggtgaccggg cggggcaatc tgaactggca
     1321 gccgacggcg gacgacttcc tgcagatcgc cggggtctgg cagggcgaga gcctgcaggc
     1381 ccagggcacc cgcgaggcgg gcgggatgat caacctcggc tatcgccgca aactgaccga
     1441 gaagctggcc ttccaggcga cggtgcggga ccttttcgac aatttcggcg acgtcaccac
     1501 gttcgagacg ccgacgttcc gcgaccggac cgagcgcacc ttcggaggac gcgcctggta
     1561 tgtgggtctg acctacgcct ttggttccgg gcctcgccgg ccgcaggatc cgcagttcga
     1621 cttctcggcg gcgccgacgc cgaactaatc ggcggctctt gatcgacggg atgcgtttcg
     1681 gccggggatt gcttatctcc ggccgatgcg tctgagagtc gtcgatctgg aaaccaccgg
     1741 tggagaccgg accgccgaga tcatcgaggt cggcatcgtc gacgtcgtgc cgctgggcga
     1801 tggcgagggg cagggctgga aggccatgcc gccggtgagc aggctgttcc ggccgcgggg
     1861 cgagatctcg ttccacgcca tggcggtgca tcacctgacg ccggcgcatt tctgcgatgc
     1921 tgatccgcat tgcgacgaat atgcgctgcg cgagatgttc ggcgccacgc ccgccgatgt
     1981 gatggtcgcg catgcggcgc ggttcgagcg gggcttcatc gccgatacgg cccatggcgg
     2041 cctgccgtgg atctgcacgg tgcgatcggc caaggcggtg tggccgcagg cgcccgggca
     2101 ttccaaccag gtgctgcgct attggcgcgg gctgaatctc gatccggcgc tggccaatcc
     2161 ggcccaccgg gcgggccccg acgcctgggt gacggcgcat ctgctgatcg atctgctgaa
     2221 ggaggcgacg gtggagcaga tgatcgcctg gacggccgag cggcggcccg cggaccgcat
     2281 cccgttcggc aagcatcgcg gcaaaccgtg gtccgagccg cccgaggact accttcgctg
     2341 gatggccgcg caggctgata tggacgccga cgccgtgtcc gccgcgcgac aggagctgga
     2401 gcgtcgcgcg ggggctctgg cgaaggcggg cgacgcgggc taaacccgtc gccgggccgc
     2461 ctttcggaga ctgtagtgaa gattgccgaa cgctggttcg ttatggcggg cgtcgcgctg
     2521 ctgggcgcga tcgcgctggc ggggctgctc gtcgccgtct atgcggcctg gctgttccac
     2581 gacctccccg acgcctccga actggccgac taccggcccg ccacggcgac ccgcgtctac
     2641 gccggcgacg gcaccctgat cggtgagttc tccgacgagc gccgcatcta cgtgccgtat
     2701 gagcaggtgc cggagccggt gatccaagcc ttccttgcgg ccgaggaccg gaacttcttc
     2761 aaccacggcg gcatcgacgt cgg
//
LOCUS       DASVVN010000663         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_103195,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000663 DASVVN010000000
VERSION     DASVVN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103195"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..>1984)
                     /gene="rnr"
                     /locus_tag="VF686_10905"
     CDS             complement(110..>1984)
                     /gene="rnr"
                     /locus_tag="VF686_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX8662364.1"
                     /translation="NKVLGVIRKSNKEVRVEPVDRRSKDVLIVPHAQAEGLRDGDLVL
                     AAIEKGDQRYGPKRGKILEKIGHEGDARAASLIAIAAHNVPMGFSEAVEKEAEDQELP
                     SLKGREDLRDLPLITIDPADARDHDDAVYAMRDDDPKNADGWVVWVAIADVAAYVRPN
                     TSLDREAREKGNSTYFPDRVEPMLPERLSNGLCSLKEGENRACMAVRMVFDKDGKKTG
                     HKFVRGLMRSQAKLSYEQAQAAIDGQTDDTTGPIMEAILYPLWNAYRTMLKGRLKRSP
                     LAIESPERRIRMNTEGQIISIEPRVSLEAHRLIEEMMIQANVCAAETLEQKKTPLLYR
                     VHDGPSQEKLFNLADFLQTIGKPWNKGEPATTKRFNKLLDETRGGDHAEVVNEVVLRS
                     QMQAIYSAENVGHFGLNLDRYAHFTSPIRRYSDLIVHRGLIRALGLGKDGLTDREVTE
                     LPAIAEQVTMTERRSMAAERAAMDRYMAAFLQERVGATFGGRITGVTRFGLFVRLEET
                     GADGLVPVSSLGGEYFHHDEKQHALIGERSGQRWTLGRTVEVRLKEATPITGGLVFEM
                     LSEPAPRDPNAPAARLGVRSRGGGDFPKRGGGRPGGPKPPKGGRPSGGLKGVRKGKRR
                     "
BASE COUNT          257 a          636 c          724 g          367 t
ORIGIN      
        1 gcccctttgc ggacccgcgt caggatcagg gtggcggcgt ccttcggtcg ctggacgccg
       61 ccgacgcgcg gggcgtcgcg cacgtcctgg tcgccgtcga acggctggat caacgccgct
      121 tgcccttgcg cacgcccttc agaccgccgg agggccgccc gcccttgggc ggcttgggtc
      181 cgccgggacg gccgccgccg cgtttgggga agtcgccgcc gccgcggctg cgcacgccca
      241 gacgggccgc gggcgcattg ggatcgcgcg gcgccggttc ggacagcatt tcgaagacca
      301 gaccgccagt gatgggcgtg gcctccttca gcctgacctc gacggtgcgg cccaaggtcc
      361 agcgctggcc cgagcgttcg ccgatcaggg cgtgctgctt ctcgtcgtga tggaaatact
      421 cgccgcccag cgacgagacc ggcaccagac cgtcggcgcc ggtctcctcc agccgcacga
      481 acaggccgaa gcgggttacg ccggtgatgc ggccgccgaa ggtggcgccg acccgctcct
      541 gcaggaaggc ggccatgtag cggtccatgg cggcgcgctc ggcggccatc gagcggcgct
      601 cggtcatggt gacctgttcg gcgatggcgg gcagttcggt gacctcccgg tcggtcaggc
      661 catccttgcc gaggcccagc gcgcggatca ggccgcggtg gacgatcagg tcggaatagc
      721 gccggatcgg cgaggtgaag tgggcgtagc ggtcgaggtt gaggccgaag tggccgacgt
      781 tctcggccga atagatggcc tgcatctggc tgcgcaggac cacctcgttg accacctcgg
      841 cgtggtcgcc gccgcgcgtc tcgtcgagca gcttgttgaa ccgtttggtg gtggccggct
      901 cgcccttgtt ccacggtttg ccgatggtct ggaggaagtc ggccaggttg aaaagcttct
      961 cctgactggg cccgtcgtgg acgcggtaca gcaggggcgt cttcttctgc tccagcgttt
     1021 cggcggcgca gacgttggcc tggatcatca tctcctcgat cagcctgtgg gcctcgaggc
     1081 tgacgcgggg ctcgatcgag atgatctggc cctcggtgtt catgcggatg cgccgttccg
     1141 ggctttcgat ggccagcggg ctgcgcttga ggcggccctt cagcatggtc cggtaggcgt
     1201 tccacagcgg atagaggatg gcctccatga tgggaccggt ggtgtcgtca gtctggccgt
     1261 cgatggcggc ctgggcctgt tcgtaggaca gcttggcctg agaccgcatc aggccgcgga
     1321 cgaatttgtg tccggtcttc ttgccatcct tgtcgaagac catgcgcacg gccatgcagg
     1381 cgcggttctc gccttccttg aggctgcaca ggccgttgga caggcgttcg ggcagcatcg
     1441 gctcgacgcg gtcggggaag taggtggagt tgcccttctc gcgcgcctcg cggtcgagac
     1501 tggtgttggg ccggacatag gcggcgacgt cggcgatggc gacccagacg acccagccgt
     1561 cggcgttctt cggatcatcg tcgcgcatgg cgtagacggc gtcgtcgtgg tcgcgggcgt
     1621 cggcgggatc gatggtgatc agcggcaggt cgcgcaggtc ctcgcggccc ttcagcgacg
     1681 gcagttcctg atcctccgcc tccttctcga cggcttccga gaagcccatc gggacgttgt
     1741 gggcggcgat ggcgatcagg gaggcggcgc gggcgtcgcc ctcgtggccg atcttttcga
     1801 ggatcttgcc gcgtttgggc ccgtagcgct ggtcgccctt ctcgatggcg gccaggacca
     1861 gatcgccgtc gcgcaggccc tcggcctggg cgtggggaac aatcagaacg tccttggagc
     1921 ggcggtcgac gggttcgacc cggacctcct tgttcgactt gcggatgacg cccagcacct
     1981 tgtt
//
LOCUS       DASVVN010000664         6322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_446470,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000664 DASVVN010000000
VERSION     DASVVN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446470"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2169
                     /gene="clpA"
                     /locus_tag="VF686_10910"
     CDS             <1..2169
                     /gene="clpA"
                     /locus_tag="VF686_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX8662365.1"
                     /translation="CNVDLTNLKAALTLYVDTDLAALATSDGEDAKPTAGFQRVIQRA
                     VIHVQSSGREEVTGANVLVAIFSERESHAAYFLQEQDMTRYDAVNYIAHGIAKKAGGS
                     GETRPAKTGSSPEDAEDAAAAKTGGEALEAYCVDLNEKSRNGKIDPLIGRLGEVERAI
                     QILCRRTKNNPLLVGDPGVGKTAIAEGLARKIVNHEVPEVLEGATIYSLDMGALLAGT
                     RYRGDFEERLKQVVKELETHDNAILFIDEIHTVIGAGATSGGAMDASNLLKPALASGS
                     LRCMGSTTYKEYRQHFEKDRALARRFQKIDVNEPTVEDTIKILKGLKTSYEGHHKLRY
                     TDAALRTAVELSARYMTDRKLPDKAIDVIDEAGASQMLLPESKRKKVIGQKEVEVVIA
                     RMARIPAKSVSKSDTEGLRALETDLNRVVFGQSSAIEQVSAAMKLARAGLRDPSKPIG
                     AFLFSGPTGVGKTEVAKQLASTLGIEMLRFDMSEYMERHTVSRLIGAPPGYVGHDQGG
                     LLTDAVDQHPHAVVLLDEIEKAHPDVYNILLQVMDNGMLTDAVGKKVDFRNTILIMTT
                     NAGAADNARASIGFGRGKVEGEDEKAIQRLFAPEFRNRLDAIVAFKALDAETIKSVVT
                     KFIIQLEAQLGDRNITIELTDEAADWLAKNGFDELYGARPLARVIQEHIKKPLADDIL
                     FGRLTRGGHVKVTLTDGKIAFDIAGPNAAQVKAVEAQAAD"
     gene            complement(2229..2534)
                     /locus_tag="VF686_10915"
     CDS             complement(2229..2534)
                     /locus_tag="VF686_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8662366.1"
                     /translation="MTDQRIDGAADTLTGQTQSGLGRMTGDSKLQIEGKLNEYKGKAK
                     DAYGRVIDGLDGLVEKAPVQYQDKARQGLDFARRKPLLTTGIVAGLAILLGGIGRRR"
     gene            2696..3277
                     /locus_tag="VF686_10920"
     CDS             2696..3277
                     /locus_tag="VF686_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEX8662367.1"
                     /translation="MKTGAVLTERQQKWFATVQANFEKATGKPLAAWVEIIKACPETA
                     PKAQTAWLKANHGIGVNHAAQILDACRPSDGPGWDEPEALRAALWKDAGSLAILEAIE
                     RVAGGVDRVISGQRKGYTSFSRTVQFAAMRPLKGGRALLGLKLDPAASARLTPAARKE
                     SWSERLIAVVELDGPEQVDAELARLFALAADNG"
     gene            complement(3282..4883)
                     /locus_tag="VF686_10925"
     CDS             complement(3282..4883)
                     /locus_tag="VF686_10925"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8662368.1"
                     /translation="MITLAALAIVIAGAVGLMAYAAAGVDRVQTAREQQLAEVRVTRM
                     LEGLTENINSSAIWNDSVSTLAGEPDLEWLQTNFGDYYADFMGHAVTLVYDRHGALIL
                     ASRDSERVAPASEQAFIDAVVPLVSQARSEAAAKHDRARSGFDAAVNHTAVVRAGADV
                     YLVGLGTVVPEDLSTPRLSADPVIVSAKPVSVLLTSLEKDLALRAPTLAEADAADSDR
                     GAIVLKGLDGAPLGKIMWTPADPGRAVLLSAAPVIGGVILLLAAITVALSLRIRGIVR
                     RLEENEADLTEARDRAEGANVAKGRFLAVMSHELRTPLNGVLGMAQVLAVGNLDAKQR
                     SHLGVLQQSGESLLKLIERILTVARLEKGELVPEPDGVDVAELIESVAADYRGAADRI
                     GLTLETTIDRSADGVWRLDGGYVRQALGHLLDNAIRHTPRGSVRLEARAAGKGLEFRV
                     IDTGVGIAPARLPDLFGKFIQADNSSTRTTEGAGVGLTLARGLVEAMGGSVRAMSTPG
                     MGSTFVIDLPADRLEDAPQADRKAA"
     gene            5065..5757
                     /locus_tag="VF686_10930"
     CDS             5065..5757
                     /locus_tag="VF686_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX8662369.1"
                     /translation="MRYVVRAALAGVLILSGAPALAQSADGAWTLEARAGVVSDYRWR
                     GTSLSGGDPALQAGATLSHANGLYGDVYVSSIEEYGFGVDGDGAEIEVTLTAGWAGAV
                     AGFDVDAGVAAYQYPDGSDVAYVEIPVQAGRTVGPWTWTLGAAFAPAQSALGDEDNRY
                     VWVGAVLAPEAWPVALEATVGREEGAWAPDGKTDWSLGARAPFGPVEIGLAWVDSNVD
                     EGAAVASLFASF"
     gene            5960..>6322
                     /locus_tag="VF686_10935"
     CDS             5960..>6322
                     /locus_tag="VF686_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEX8662370.1"
                     /translation="MSASIDLPVVVFSGHRLIARGAVGEVLGDIRSGAEADAGLLVFD
                     AATGRVVDLDLRGTLDEAKARLTPAPEAEKRGPGRPRLGVTAREVTLLPRHWDWLSAQ
                     PGGASVALRKLVEGALRDA"
BASE COUNT         1043 a         2117 c         2211 g          951 t
ORIGIN      
        1 tgcaacgtcg acctgaccaa tctcaaggcg gcgctgaccc tttacgtcga caccgacctg
       61 gcggccctcg ccaccagcga cggtgaggac gccaagccga ccgccggctt ccagcgcgtc
      121 atccagcgcg ccgtcatcca cgtccagagc tcgggccgcg aggaggtcac cggcgcaaac
      181 gtgctggtcg ccatcttcag cgagcgcgaa agccatgccg cctacttcct gcaggagcag
      241 gacatgacgc ggtacgatgc ggtcaactac atcgcccatg gcatcgccaa gaaggccggc
      301 ggttcgggcg agacgcgtcc ggccaagacc ggctcgtccc ctgaggacgc cgaggacgcg
      361 gccgccgcca agaccggcgg cgaagcgctg gaggcctatt gcgtcgacct caacgagaag
      421 tcgcgcaacg gcaagatcga ccccctgatc ggccgcctcg gcgaggtcga gcgggcgatc
      481 cagatcctgt gccgccggac caagaacaac ccgctgctgg tcggcgaccc gggtgtcggc
      541 aagaccgcca tcgccgaagg cctggcccgc aagatcgtca accacgaagt gcctgaggtg
      601 ctggaaggcg ccaccatcta cagcctcgac atgggggcgc tgctggccgg cacccgctat
      661 cgcggcgact tcgaggagcg gctgaaacag gtcgtcaagg aactggagac gcacgacaac
      721 gccatcctgt tcatcgacga gatccacacg gtcatcggcg cgggcgcgac ctcgggcggg
      781 gccatggacg cctccaacct gctcaagccg gctctggcgt ccggctctct gcgctgcatg
      841 ggctccacca cctacaagga gtaccgccag catttcgaga aggaccgggc cctggcccgc
      901 cgcttccaga agatcgacgt caacgagccg acggtcgagg atacgatcaa gatcctcaag
      961 ggcctgaaga cctcgtacga gggtcaccac aagctgcgct acacggacgc ggccctgcgc
     1021 acggcggtcg agctgtcggc ccgctatatg accgaccgta aactgccgga caaggcgatc
     1081 gacgtcatcg acgaggcggg cgccagccag atgctgctgc ctgaatcgaa gcgcaagaag
     1141 gtcatcggcc agaaggaggt cgaggtcgtc atcgcccgca tggcccgcat cccggccaaa
     1201 tcggtctcca agtccgacac cgagggcctg cgcgcgctgg agaccgatct caaccgggtc
     1261 gtcttcggcc agtcctcggc catcgagcag gtctccgcgg ccatgaagct ggcccgcgcg
     1321 ggcctgcgag atccgagcaa gccgatcggc gccttcctgt tcagcggccc gacgggcgtc
     1381 ggcaagaccg aggtggccaa acagctggcc tcgaccctcg gcatcgagat gctgcgcttc
     1441 gacatgtccg aatacatgga gcggcacacc gtcagccgcc tgatcggcgc gcctccgggc
     1501 tatgtcggcc atgaccaggg cgggctgctg acggacgccg tcgaccagca tccgcacgcc
     1561 gtggtcctgc tcgacgagat cgagaaggcc cacccggacg tctacaacat cctgctgcag
     1621 gtgatggaca acggcatgct gaccgacgcg gtcggcaaga aggtcgattt caggaacacc
     1681 atcctgatca tgaccaccaa cgccggggcc gccgacaacg cccgcgcctc catcggcttc
     1741 ggccggggca aggtcgaggg cgaggacgaa aaggccatcc agcgcctgtt cgcgccggag
     1801 ttccgcaacc gtctggacgc catcgtcgcc ttcaaggcgc tggacgccga aacgatcaag
     1861 tcggtggtga cgaagttcat catccagctg gaggcccagc tgggcgaccg caacatcacc
     1921 atcgaactga ccgacgaggc ggccgactgg ctggccaaga acggcttcga cgaactgtat
     1981 ggcgcgcggc ctctggcccg ggtcatccag gagcacatca agaagccgct ggccgacgac
     2041 atcctgttcg gacgtctgac gcgcggcggc catgtgaagg tcaccctgac cgacggcaag
     2101 atcgccttcg acatcgccgg ccccaacgcc gcacaggtca aggcggttga ggcccaggcc
     2161 gcggactgac acgaagaagg cccgggcgtc gctgcccggg ccttttgtcc gtctgaagcc
     2221 ggagcgcctt agcggcggcg gccgatcccg cccagcagga tggccagacc ggcgacgatg
     2281 ccggtggtca gcagcggctt gcggcgggcg aagtccagac cctgacgcgc cttgtcctga
     2341 tactgaacgg gcgccttctc gacgaggccg tccagcccgt cgatgacccg gccataggcg
     2401 tccttcgcct tgcccttgta ttcattcagc ttgccctcga tctgaagctt gctgtcgccg
     2461 gtcatgcggc cgaggccgga ctgggtctgg ccggtcaggg tgtctgcggc gccgtcgatg
     2521 cgttggtcgg tcatgagatg tccttgctgc gaatggggcg gggaggcctg caccggtagt
     2581 gcatgtcggg acctgcggtt cctgcggcaa ggcgtccggg ccccaatctt ttcgccccgc
     2641 cttgaccccg cgccgcgagc ggcgacgatg gcggcgggga agtgggagct gaggcatgaa
     2701 gaccggcgcg gtgctgaccg aacggcagca gaaatggttc gccacggtgc aggccaattt
     2761 cgagaaggcc acgggcaagc ccctcgcggc ctgggtcgag atcatcaagg cctgccccga
     2821 gacggcgccc aaggcccaga ccgcttggct gaaggcgaac cacgggatcg gggtcaatca
     2881 tgcggcgcag atcctggacg cctgccgccc ctccgacggc cccggctggg acgagcccga
     2941 ggccctgcgt gcggcgcttt ggaaggacgc cggttcgctc gcgatcctgg aggcgatcga
     3001 gcgcgtcgcc ggcggcgtgg accgcgtcat ttctggccag cgcaagggct acacctcctt
     3061 ctcgcggacg gtgcagttcg cggcgatgcg gccgctgaag ggcgggcgcg ccctgctggg
     3121 gctgaagctg gatccggcgg cttcggcgcg gctgacgccg gctgcgcgca aggaaagctg
     3181 gtccgagcgg ctgatcgcgg tcgtcgagct ggacgggccg gagcaggtgg acgcagagct
     3241 ggcgcggctg ttcgccctgg ccgccgacaa cggttgaagc gtcaggcggc cttccggtcg
     3301 gcctgaggcg cgtcttccag ccggtcggcc ggcaggtcga tcacgaaggt ggagcccatg
     3361 ccgggcgtac tcatggcgcg cacgcttcct cccatggcct cgaccagccc ccgggccagg
     3421 gtcaggccaa cgcccgcgcc ctccgtggtc cgggtggagg aattgtcggc ctggatgaat
     3481 ttgccaaaca ggtcgggcag gcgcgcgggg gcgatgccga cgccggtgtc gatgacacgg
     3541 aactcaagtc ccttgcccgc agcgcgggcc tcgagccgga ccgaaccacg gggcgtatgc
     3601 cggatggcgt tgtcgagcag atggcccagg gcctggcgga catagccgcc gtccagtcgc
     3661 cacacgccgt cggcgctgcg gtcgatggtg gtctcaagcg tcagaccgat ccgatcggcg
     3721 gcgccccggt agtcggccgc gacggattcg atcagctccg cgacatccac cccgtcgggt
     3781 tcgggtacca gctcgccctt ttccagccgg gccacggtca ggatgcgttc gatcagtttg
     3841 agcaggctct cgccggactg ctgcaacacg cccagatggc tgcgctgttt ggcgtcgaga
     3901 ttgcccacgg ccagaacttg ggccatgccc agaaccccgt tcaggggcgt gcgcagttca
     3961 tggctcatta cggccaggaa gcggcccttg gcgacattgg ccccctcggc gcggtcgcgc
     4021 gcctcggtca ggtccgcctc attctcttcc agtcggcgaa cgatgcccct gatgcgcagc
     4081 gaaagggcga cggtgatggc ggccagcagc aggatgacgc ctccaatgac cggagcggcg
     4141 ctgagaagca cggcccggcc aggatccgcg ggcgtccaca tgatcttgcc caagggcgcg
     4201 ccgtccaatc ctttcaggac gatggcgccg cggtcgctgt cggctgcatc ggcctccgca
     4261 agggtgggcg cccgaagcgc cagatccttc tccagcgagg tcagcagcac ggaaaccggc
     4321 ttggcggata cgatgaccgg atcggccgac agacggggcg tggagaggtc ttcgggcacc
     4381 acggtgccga ggccgaccag atagacgtcc gcgcccgcgc gcaccacggc cgtatgattg
     4441 acggcggcgt cgaagcccga gcgcgcccgg tcatgcttgg ccgcggcctc gctgcgcgcc
     4501 tgcgacacca gcggcaccac ggcgtcgatg aaggcctgtt cgctggccgg cgccacgcgt
     4561 tcgctgtccc gcgaggccag gatcaaggcg ccatgccggt cgtagacgag ggtgacggcg
     4621 tgacccatga agtcggcgta gtagtcgccg aaattggtct gcagccattc cagatccggc
     4681 tcgccggcca aggtgctcac ggagtcgttc cagatggccg aggagttgat gttttcggtc
     4741 aggccctcaa gcatgcgggt gacccggacc tcggccagtt gctgctcgcg ggccgtctgc
     4801 acccggtcga cgcccgccgc ggcataggcc atcaggccga cagcgccggc gatcacgatg
     4861 gccagcgccg caagcgtgat cacgacgatc cgcctgagtt ccttgcgccc cgaggttgca
     4921 accttccctg acatgcagcg accgtgtgcc gccgtctgat aaagtccggt gacggaagaa
     4981 ggttattctc ctgttcgcca tcttcgcatc cggatggtta agcggccgat ccgaggatgg
     5041 attccgaaga ggggagttct tccaatgcga tacgtcgttc gagcggcctt ggccggggtt
     5101 ctgatcctgt ccggcgcgcc ggcgctggcg cagtcggcgg atggggcctg gacgctcgag
     5161 gcgagggcgg gcgtggtgtc cgactatcgc tggcgcggaa cctcgctgtc gggcggcgac
     5221 ccggccctgc aggccggcgc gaccctgagc catgccaacg gcctgtatgg cgacgtctat
     5281 gtgtcctcga tcgaggaata cggcttcggc gtcgatggcg acggggcgga aatcgaggtc
     5341 accctgacgg caggctgggc tggagccgtg gccggcttcg acgtcgacgc cggggtcgcc
     5401 gcttatcagt atccggacgg ctcggacgtg gcctatgtcg agatcccggt gcaggccgga
     5461 cgcacggtcg gaccctggac ctggacgctg ggggccgcct tcgcgccggc gcagtcggcg
     5521 ctgggcgacg aggacaaccg ctatgtctgg gtcggtgcgg tgctggcgcc ggaggcctgg
     5581 ccggtggcgc tggaggcgac ggtgggccgg gaggaggggg cctgggcccc cgacggcaag
     5641 acggactggt cgctgggggc gcgggcgccg ttcggaccgg tggagatcgg cctcgcctgg
     5701 gtcgacagca atgtcgacga gggcgcggcg gtggcgagcc tgttcgccag cttctgatat
     5761 ttacccgggc gatattgacg cggtcggttt gacccgggta gaaagcggcc agttcgggtg
     5821 cgtcggtgaa aaacaccgtc cgaagaatcc gaagagtcca ctgagtccac tcgatccacc
     5881 gcgccctccc tctgaaaaaa caggataggc cgcccctacg tcagaaacgg tcgcatccgc
     5941 ggccgctccg gagcccgcca tgtccgcatc catcgatctg cccgtcgtcg tcttttcggg
     6001 ccaccgcctg atcgcccgcg gggcggtggg ggaggtgctg ggcgacatcc ggtcgggcgc
     6061 cgaggcggat gcgggcctgc tggtgttcga cgccgcgacg ggccgggtgg tcgatctgga
     6121 cctgcgcgga accctggacg aggcgaaggc ccggctgacg cccgcgcccg aagcggagaa
     6181 gcgcgggccg ggccggccga ggctgggcgt cacggcgcgc gaggtgactc tgctgccgcg
     6241 ccactgggac tggctgtccg cccagcccgg cggcgcctcg gtggccctgc gtaaactggt
     6301 cgagggcgcc ctgcgcgacg cc
//
LOCUS       DASVVN010000665         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_103267,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000665 DASVVN010000000
VERSION     DASVVN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103267"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF686_10940"
     CDS             complement(<1..387)
                     /locus_tag="VF686_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX8662371.1"
                     /translation="MKVAQVLLPLPLEEAFDYAVPEGLEAEAGAHVCVPLGPRLAHGV
                     VLSAAERMGVNRPLKAVEGVLDEPALPEKTLEFVQWAARYAVDAPGCALAIALRGARA
                     PRPRPERRLEATGMPPSNPTPARLKVL"
     gene            461..1114
                     /gene="fsa"
                     /locus_tag="VF686_10945"
     CDS             461..1114
                     /gene="fsa"
                     /locus_tag="VF686_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX8662372.1"
                     /translation="MQLFIDTADVGLLRELAQTGLVDGVTTNPSLIAAGGRNFIEVVG
                     EICSFIDGPVSAEVAAMDFEQILREGQRLAEIAPNVVVKVPLTWDGLRAVREFSVQGI
                     ATNVTLCFSTSQALLAAKAGASFISPFIGRLDDHGAEGMDLIQEIRIVYDNYDFDTEI
                     LAASIRTPAHVAQAAILGADCATIPPKVFQELVKHPLTTKGLQQFTEDWAKTGQSIL"
     gene            1125..>1609
                     /locus_tag="VF686_10950"
     CDS             1125..>1609
                     /locus_tag="VF686_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX8662373.1"
                     /translation="MTKAADKMPADMASLDWEAVKAWLRDNPDLLREDDDLLQALGLH
                     AGNVVALMPPSIAQLKEERDRALRARRSLEQLAKANFQVQEQAQAAVLSLLEARSNSD
                     LARRVDETARNRFNLVGGAIALEGPGPVPVGWRELPPDGADALLGPGDYRLGQVPEAA
                     AL"
BASE COUNT          243 a          590 c          556 g          220 t
ORIGIN      
        1 cagcaccttc agccgcgccg gcgtggggtt cgacggcggc atgccggtgg cttccagccg
       61 ccgctccggc cgcggccgtg gcgcgcgggc gccgcgcagc gcgatggcca gggcgcagcc
      121 gggcgcgtcg accgcgtagc gcgccgccca ctgcacgaac tccagcgtct tctccggcaa
      181 cgcgggctcg tcgagcacgc cctcgaccgc cttcagcggc cggttgacgc ccatccgctc
      241 ggcggcgctc agcaccacgc cgtgcgccag tcgcgggccg agcggcacgc agacgtgcgc
      301 gccggcttcg gcctccagcc cttcggggac ggcgtagtcg aacgcctcct ccaggggcag
      361 cggcagcagg acctgggcga ccttcatgga cctatgggtt gcggagcgcg gccgattggc
      421 cgattgccgg ggtggagttc aacaagccga aggacccggc atgcaactgt tcatcgacac
      481 cgccgatgta ggccttctgc gcgagttggc ccagaccggc ctggtcgacg gcgtcaccac
      541 caacccgtcg ctgatcgccg ccggcgggcg caacttcatc gaggtcgtcg gtgagatctg
      601 cagcttcatc gacggcccgg tcagcgccga ggtggccgcc atggatttcg agcagatcct
      661 gcgcgagggc cagcggctgg ccgagatcgc ccccaacgtc gtcgtcaagg tgccgctgac
      721 ctgggacggc ctgcgggcgg tgcgcgagtt ctcggtgcag ggcatcgcca ccaacgtgac
      781 gctgtgcttc tcgacgtcgc aggcgctgct ggcggccaag gcgggcgcca gcttcatctc
      841 gccgttcatc ggccgcctgg acgaccacgg ggcggagggg atggacctga tccaggagat
      901 ccggatcgtc tacgacaact acgacttcga caccgagatc ctggccgcct cgatccgcac
      961 cccggcccac gtggcccagg cggcgatcct cggcgccgac tgcgcgacca ttccgcccaa
     1021 ggtgttccag gagctcgtaa agcacccgtt aaccacgaag gggcttcaac agttcaccga
     1081 agactgggcg aagaccggcc agtcgatcct ttagaaggtc gcgcttgacc aaggccgcgg
     1141 acaagatgcc ggccgacatg gccagcctgg actgggaagc ggtcaaagcc tggctgcgcg
     1201 acaaccccga cctgcttcgc gaggacgacg atctgctgca ggcgctcggc ctgcacgcgg
     1261 gcaacgtggt ggcgctgatg ccgcccagca tcgcgcagct gaaggaggag cgcgaccgcg
     1321 cgcttcgcgc ccggcgctcg ctcgagcagc tcgccaaggc caatttccag gtccaggagc
     1381 aggcgcaggc cgccgtgctc agcctgctcg aggcgcgcag caacagcgac ctcgcgcgcc
     1441 gggtcgacga gacggcccgc aaccgcttca acctggtcgg cggcgccatc gccctcgagg
     1501 gccccggccc cgttcccgtg ggctggcgcg agctgccgcc cgacggggcc gacgccctgc
     1561 tcggcccggg cgactatcgc ctcggccagg tccccgaggc ggccgccct
//
LOCUS       DASVVN010000666         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_52352, whole
            genome shotgun sequence.
ACCESSION   DASVVN010000666 DASVVN010000000
VERSION     DASVVN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52352"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2446)
                     /locus_tag="VF686_10955"
     CDS             complement(<1..>2446)
                     /locus_tag="VF686_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX8662374.1"
                     /translation="PPPQMVFSLDNPGAPSTIYVSADPTKNHLSLHLSCTIDAQLSHA
                     VLVPFGQAGSAPGALIYLDLSPLGLDAALFDTLKLEASGWEFKAYSADNMIALTPTLA
                     AVMKANQVLTFALKNFALATAPGASASLQLYAFNVPGVADGALPASTSKTVAFVAPPA
                     GHGDLRTVLSATLTPGAVVSSVSGYPEVSNKLSLTLAQIGGQPTATTGDSSVFTLSVA
                     YSEDVNGFGALMNAKEGKAISIPVPEGSDWHTANVDGTSGRSWQLTPPKKTALPTAGG
                     DSLEFDITPIVTYFQPGATIVLLSYSGVPGYDDGSFPITVLKRPHVALSKPTATPPIS
                     TLDNDGNAAVTLSWTASNATKVTLFPGQIDVTGRTSFPTTVTDTQIFTLNAEGERPGN
                     VDNTAIANVTATVLPVINSFTASPPAIYVGDFGNGYRTLLAWNVNTNRDVLVTSSTTG
                     QHGPFLAAQEEPFFLVGPQLLTVAPRTGKADPKVSRSLVVSPFTVTAQQVPTASGTAF
                     AAAPASAPFVAMTSGSGVLLLSTATYKTLATVSAGGSPQGIAFSADGATMFVANSGDG
                     TVGIYSVAASQTAPGFAVTSTGTVQVGGAPQSLAVAGDGTVYVSIDKGATAAGSVAIL
                     AKGNQGYSVSQTATVGIAPRGIALLPNGAALFVANKGGGSLSLVTPGSPPSVSTPITG
                     LNGPTDVGVSPDGKVLLVSSAGDNNVYIYNAVYPSTGSRRALAVPGAGRIGMMPGGAY
                     ALVTGTGPNRAVLINYALATISATTPLSGPSSGVAVTPDGGFAVVAGSGTILTFAQYA
                     QVSATTPAGGQITNVAV"
BASE COUNT          384 a          759 c          922 g          381 t
ORIGIN      
        1 acggcgacat tggtgatctg gccgccggcc ggagtggtcg ccgaaacctg cgcatattgg
       61 gcgaaggtga ggatcgtgcc cgaccccgcg accacagcga agcctccgtc gggggtcacc
      121 gccacccccg aggaaggtcc ggagagcggc gtggtggccg agatggtggc gagcgcatag
      181 ttgatcagca ccgcccggtt cggccccgtg ccggtgacca aggcataagc gccgccgggc
      241 atcatcccga tccggccggc gcccgggacc gccagggccc ggcgcgagcc cgtcgaggga
      301 tagaccgcat tgtagatata gacattgttg tcgccggccg aggagacgag cagcaccttg
      361 ccgtcgggtg aaactccgac atcggtgggc ccgttgaggc cggtgatcgg cgtcgacacc
      421 gagggcggcg agccgggagt gacgagcgac agcgagccgc cacccttgtt ggcgacgaac
      481 aaagcggcgc cgttgggcag caaggcgata ccgcgcggcg cgatccccac cgtcgccgtc
      541 tgcgacaccg aatagccctg gttgcccttg gcgaggatgg cgacgctgcc cgcggccgtg
      601 gcgcccttgt cgatgctgac gtagacggtg ccgtcgccgg cgacggcgag cgactgcggc
      661 gctccgccca cctggacggt tccggtcgag gtcaccgcga agcccggcgc cgtctgcgag
      721 gccgcgaccg aatagattcc gaccgtgccg tcgccggaat tggccacgaa catggtggct
      781 ccgtccgcgg agaaggcgat gccctgcggc gagccgccgg cgctcaccgt cgccagcgtc
      841 ttgtaggtcg cggtcgacag cagcagcact ccggagcccg aggtcatggc gacgaacggc
      901 gcggaagcgg gcgccgccgc gaaggcggtc ccgctcgccg tcggcacctg ctgggcggtg
      961 accgtgaacg gcgagacgac caggcttcgg ctgaccttgg gatcggcctt gccggtcctc
     1021 ggcgcgacgg tcagcaattg cgggccgacg aggaagaagg gctcttcctg ggccgccaga
     1081 aaggggccgt gctggccggt ggtgctgctc gtgaccagga cgtcgcgatt ggtgttgacg
     1141 ttccaggcca gcagtgtccg gtagccgttg ccgaagtcgc cgacgtagat ggccggcggc
     1201 gaggcggtga agctgttgat caccggaagc acggtcgcag tgacgttggc gatcgcggtg
     1261 ttgtcgacgt tgcccgggcg ctcgccctcg gcgttcaggg tgaagatctg ggtatcggtg
     1321 accgtggtcg ggaaggaggt cctcccggtg acgtcgatct ggcccgggaa gagagtcacc
     1381 ttggtcgcgt tggacgcggt ccagctcagt gtgacggcgg cattgccgtc gttgtcgaga
     1441 gtcgagatcg gcggggttgc cgtaggcttg ctgagcgcca cgtgaggccg cttcagcacc
     1501 gtgatcggga aggagccgtc atcatagccg ggcacgcccg aatagctcag cagcacgatc
     1561 gtcgcgccgg gctggaaata ggtgacgatg ggggtgatgt cgaactccag cgaatcccct
     1621 ccggcggtcg gcagcgccgt tttcttgggc ggggtcagct gccacgagcg gccgctcgtt
     1681 ccgtcgacgt tggcggtgtg ccagtcgctt ccctccggaa ccgggatgga gatggccttg
     1741 ccctccttgg cgttcatcag ggcgccgaag ccgttcacgt cctccgaata ggcgaccgaa
     1801 agggtgaaga cgctgctgtc cccggtcgtc gcggtcggct gcccgccgat ctgggcgagg
     1861 gtcaggctga gcttgttgga gacctccgga tagccggaca ccgaggagac gaccgcgccg
     1921 ggcgtcaggg tcgcgctcag gacggtccgc agatcgccgt ggccggccgg cggagcgacg
     1981 aaggcgaccg tcttgctggt cgaggcggga agggcgccgt cggccactcc ggggacgttg
     2041 aaggcgtaga gctggaggct ggccgaggcg cccggcgcgg tcgccaaagc gaaattcttg
     2101 agcgcgaagg tcagcacctg gttcgccttc atcaccgccg ccagggtggg cgtgagggcg
     2161 atcatgttgt cggcgctata ggccttgaac tcccagccgc tggcctcgag cttcagggtg
     2221 tcgaacaagg cggcatcgag cccgagcggc gacaggtcga gatagatgag ggcccccggc
     2281 gcgctcccgg cctgcccgaa cggcaccagg acggcgtggc tgagctgggc gtcgatcgtg
     2341 cagctcaggt gcagcgacag atggttcttg gtcggatcgg cgctgacgta gatggtgctc
     2401 ggcgcaccgg gattgtcgag cgagaagacc atctgcggcg gcggtg
//
LOCUS       DASVVN010000667         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_103628,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000667 DASVVN010000000
VERSION     DASVVN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103628"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..835
                     /locus_tag="VF686_10960"
     CDS             107..835
                     /locus_tag="VF686_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8662375.1"
                     /translation="MPTGDEIVVSIERTFERGPIEREHVDRLNELRPHLVRAALVTAR
                     LGLQRAKGATDALTALGLPALLVDETGRVVEANPLIEALPEKVRFGINNRLILADAKA
                     NETLASALAQIETLPAAASRTFALRDEAGQATTLLHIMPIRRSAHDVLGRSYALVLLT
                     PLDANRGAPSADLMRTLFDLTPSEARVAQGIAGGRSMEDIADQGGVAITTVRSQLRRV
                     FEKTGCTRQAEVAALLARVTIGTR"
     gene            976..1542
                     /locus_tag="VF686_10965"
     CDS             976..1542
                     /locus_tag="VF686_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662376.1"
                     /translation="MSACLALAVTLSGPAQAQRRGEPQLQVSYPTDGSLTCEQLTIEI
                     TRMEEIAGISAQNARNAENQGAIANGATSVAINAALYSGALGAVPGLGLFANAAGAAA
                     RRNAEARARAEAERIRIAEQRRSLLSGIYQGRQCGVPAAPVAPEPVMSVQAAPVEAAP
                     STEPTTDAAAPAEVSAPPAVPTAPATRS"
     gene            1604..1960
                     /locus_tag="VF686_10970"
     CDS             1604..1960
                     /locus_tag="VF686_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8662377.1"
                     /translation="MNTSLRCALALGIAAVLSGCASDLVTGGPLIRPGEPQGAIRVVN
                     ATDDQLHAVLISTCSASTYGLNRLPGGMAIPSGGAYSFTVSAGCWDVSGGTLGVGDAR
                     GRIQVAPGGTSTIRIY"
     gene            complement(2029..>2811)
                     /locus_tag="VF686_10975"
     CDS             complement(2029..>2811)
                     /locus_tag="VF686_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX8662378.1"
                     /translation="EFAHQWWAHQIVGSNQQGATSLSETLAQYSALMVMEKKYGKDQI
                     RRFLKQELDRYLRARGTERLEELPLMRVENQQYIHYQKGGLVMYLLRDQIGEDAVNRA
                     LRRVLAQYAFKGAPWPRSLDLVAAIRAEAPADRQALITDLFEKITLYDVKTTGVTSTR
                     RADGRWDVTVTVDARKLYVDGKGVETAAPLNETFDIGLFTAEPGKGAFNARNVVLLER
                     RPIRSGVQTLRFVTARAPSFGGVDPYNKWIDRNSDDNVKAVD"
BASE COUNT          433 a         1030 c          904 g          444 t
ORIGIN      
        1 cgtcgagcag gatttctggg acaaggacca gctcgagaaa gacccgatgt accgcgacct
       61 gttccgcccc cacgggctgg gctggtcgac ctagaccggc ctgcgcatgc cgaccggcga
      121 cgagatcgtg gtcagtatcg aacgcacctt cgagcgcgga ccgatcgagc gtgagcatgt
      181 cgatcggctg aacgagctgc ggccgcatct ggtgcgcgcc gccctggtca ccgcgcgtct
      241 cgggctgcaa agggccaagg gcgcgaccga cgccctgacc gcgctcggcc tgcccgccct
      301 gctcgtcgac gagacggggc gggtggtcga ggccaatccc ctgatcgagg ccctgccgga
      361 gaaggtcagg tttggcatca acaatcgtct gatcctcgcg gacgccaagg ccaacgaaac
      421 cttggcctcg gccctcgccc agatcgagac cctgcccgcc gccgcctccc gcaccttcgc
      481 ccttcgcgac gaggccggtc aggcgacgac cctgctgcac atcatgccga tccggcgctc
      541 ggcgcacgac gtgctggggc gcagctacgc cctggtgctg ctgacgccac tcgacgccaa
      601 ccggggtgcg ccgtcagcag acctgatgcg gacgctgttc gacctcaccc cctccgaggc
      661 ccgggtggcg caagggatcg ctggcggcag gagcatggag gacatcgccg accaaggcgg
      721 cgtcgccatc accaccgtcc gctcccagct gcgccgcgtc ttcgagaaga ccggctgtac
      781 ccgccaggcc gaggtggcgg ccctgctcgc ccgcgtgacg atcggaaccc gctgaaggcc
      841 gccgataaat ctttcaggct tcatccattt ggatgacgcc gcgagccgtc agacagggtg
      901 aagctccctt cacaaaccgg cgctttctgg cgctgtcggg gagcctgaac catggccaac
      961 ttcaatcgtg cagcaatgtc agcctgtctc gcgctggccg tgaccttgtc cggcccggcg
     1021 caggcccagc gtcgcggcga accgcagctg caggtgtcct atcccacgga tggaagcctc
     1081 acctgcgagc agctgacgat cgagatcacc cggatggagg agattgccgg catctcggcg
     1141 cagaacgccc ggaacgccga aaatcaaggc gccatcgcca atggcgcgac cagcgtggcc
     1201 atcaacgcgg ccctctactc cggcgcgctc ggcgccgtcc ccggcctggg tctgttcgcc
     1261 aacgccgccg gcgccgcagc ccgccgcaat gcagaggccc gcgcccgcgc cgaggccgag
     1321 cggatccgga tcgccgaaca gcgccgcagt ctgctcagcg gcatctatca gggccgtcag
     1381 tgcggcgtac ccgccgcgcc cgtggcgccg gagccggtca tgtccgttca agccgcaccg
     1441 gtcgaggccg cgccttcgac cgagccgacc accgacgccg cggccccggc cgaggtttcc
     1501 gcgcccccgg ccgtcccgac cgcgcccgcg acccgctcct gacctgccct caccgggctc
     1561 ggcgtcaggg gatcagagca cccttccaga ttggaaaaga ccaatgaaca cctctcttcg
     1621 ctgcgccctg gcgctcggga tcgccgccgt cctttcaggc tgcgcctccg atctggtcac
     1681 cggaggtccg ctgatccggc cgggcgaacc ccaaggcgcc attcgcgtgg tcaacgccac
     1741 ggacgatcag ctgcacgccg tcctgatctc cacctgctcg gcctccacct atggcctcaa
     1801 ccggcttccc ggcggcatgg cgatcccgtc aggcggcgcc tacagcttca ccgtatccgc
     1861 cggatgctgg gacgtgtccg ggggcacgct cggcgtcggc gatgcgcggg gacgcatcca
     1921 ggtggccccg ggcggaacca gcacgatccg gatctactga ccccccggag ccggggcagc
     1981 cgtcgcccgt cgcggcgcgg ttgtccccga gtccggggcg tccgcccgct agtccaccgc
     2041 cttcacattg tcgtccgagt tgcggtcgat ccacttgttg tacggatcga cgccgccgaa
     2101 gctgggggct cgcgcagtca cgaaccgtag cgtctgcacc cccgaccgga tcggccggcg
     2161 ttccagcagt acgacgttgc gcgcgttgaa ggcgcccttc cccggctcgg cggtgaacag
     2221 gccgatgtca aacgtctcgt tcagcggcgc ggcggtctcc acccccttgc cgtcgacata
     2281 gagtttgcgc gcatcaacgg tgaccgtgac atcccaccgt ccgtccgccc gcctggtcga
     2341 ggtcacgccc gtggtcttga cgtcgtacag ggtgatcttc tcgaacaggt cggtgatcag
     2401 ggcctgtctg tcggctggag cctcggcgcg gatggccgcc accagatcca gtgagcgcgg
     2461 ccaaggggcg cccttgaagg cgtattgcgc cagcactcgg cgcagggcgc ggttgaccgc
     2521 gtcctcgccg atctgatcgc gcagcaggta catgaccagc ccgcccttct ggtagtgaat
     2581 atactgctgg ttctcgaccc gcatcagggg cagttcctcc agccgttcgg tgccgcgggc
     2641 gcgcaggtag cggtccagtt cctgcttcag gaagcggcgg atctgatcct tgccgtactt
     2701 cttctccatg accatcaggg ccgaatactg ggccagggtc tccgacaggg aggtggcgcc
     2761 ctgctggttc gagccgacga tctggtgcgc ccaccattgg tgcgcgaact c
//
LOCUS       DASVVN010000668         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_209660,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000668 DASVVN010000000
VERSION     DASVVN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209660"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF686_10980"
     CDS             <1..224
                     /locus_tag="VF686_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8662379.1"
                     /translation="LEQAVAAAGRAHLLDREAIRARFDLRFSATAMARRYLDVYGDLL
                     AKRPFAEEPLDDVVTPLRGLEGRSFAANA"
     gene            334..>2417
                     /locus_tag="VF686_10985"
     CDS             334..>2417
                     /locus_tag="VF686_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX8662380.1"
                     /translation="MDDAYTVQTTALDTSETDGLERLMALKDADTFAVADGWGDMKGG
                     ADGLFDHDTRILSRLILTVGLARPSRLSSGVSQDNVYFTCHSTNRPLPPMGGRSAPAG
                     VLHIERRRFVWNRRMFERVRMVNHGIEDMLLPLALDFAADFADIFQVRGTLREKRGIT
                     ETPTHDGRRVTFAYVGLDGVRRTSCLAFSEPPARLSGTRAEFMFSLPKGKRCDLYVEC
                     GPDSCEQPDEARYRVNAVAARLAMRTRRRRGASLRGPRSPRFNDWLDQSRADVALLTT
                     DLPTGPYPYAGVPWFSTPFGRDGIITAWQMLWLDPSLAKGVLTYLAMRQATEFSAFQD
                     SAPGKIMHETRGGEMSALGEVPFGLYYGGVDTTCLFVALAGAYARRTGDFALIRKLWP
                     NLIAAVGWMKDHGDIQGDGLISYQRGADTGLSNQGWKDSEDSIFHADGRFPKGPVALL
                     EVQGYAYAAWQAMAELGAAVGDAGALEWAAQAERVRALVEDRFWMEDEGFYAIALDGD
                     GEPCRSIGSNAGHLLFTGLPSAERAKRVTKRLLGAEFRSGWGVRTLATGQARFNPMSY
                     HNGSVWPHDTAMAAAGMARYGERRAVALLLGEIYASAAHFHLRLPELFCGFERAPGEP
                     PIAYPVACLPQAWAAGSVFLMLQAALGVNIDAVAGTVEIDDPVLPSGIDRLNVTNLQV
                     GDGVVDLAFQQMG"
BASE COUNT          347 a          838 c          858 g          374 t
ORIGIN      
        1 cgctggaaca ggcggtcgcc gccgccgggc gggcgcatct gctggaccgc gaggcgatcc
       61 gcgcccggtt cgatctgcgc ttctcggcca cggccatggc gcggcggtat ctggacgtct
      121 acggcgactt gctggccaag cggccgttcg cggaagagcc gctggacgat gtggtgacgc
      181 cgctgcgcgg gctggaaggc cgcagcttcg ccgccaacgc ctgatcgggg ctgaacccct
      241 ccgcccttcg tccattaccg tacgcaggcg ccatcaagcg tccccattcg ggtgagagct
      301 tccgccgatc ctgaaccgcc ccctggagcc ttcatggacg acgcctatac cgttcagacc
      361 accgctctcg acacgtcgga aaccgacggg ctggaacggc tgatggcgct gaaggacgcc
      421 gacaccttcg cggtcgcgga cggctggggc gacatgaagg gcggggccga cggcctgttc
      481 gaccatgaca cccgcatcct ctcccggctg atcctcaccg tcggactggc gcggccgtcg
      541 cggctgagtt cgggcgtcag tcaggacaat gtctatttca cctgccactc gaccaaccgg
      601 cccctgccgc cgatgggcgg gcgctcggcc ccggcggggg tgctgcatat cgagcgccgg
      661 cgcttcgtgt ggaaccggcg gatgttcgag cgggtgcgga tggtcaacca cggcatcgag
      721 gacatgctgc tgccgctggc gctcgatttc gccgccgact tcgccgacat cttccaggtg
      781 cgcggcaccc tgcgcgagaa gcgcggaatc accgaaacgc cgacgcacga cgggcggcgg
      841 gtgaccttcg cctatgtcgg gctggacggg gtgcggcgga ccagctgcct ggccttttcc
      901 gaaccgcccg cccggctcag cgggacgcgg gccgagttca tgttcagcct gcccaagggc
      961 aagcgctgcg acctctatgt cgaatgtggc ccggacagct gcgagcagcc ggacgaggcg
     1021 cgctacaggg tcaacgccgt ggcggcgcgg ctggccatgc gcacccggcg gcggcgcggg
     1081 gcctcgctgc gggggccgcg cagtccgcgc ttcaacgact ggctggacca gagccgcgcc
     1141 gacgtggccc tgctgaccac cgacctgccg accggccctt acccctatgc cggcgtcccg
     1201 tggttctcga cgccgttcgg gcgcgacggg atcatcaccg cctggcagat gctgtggctg
     1261 gatccgtcgc tggccaaggg ggtgctgacc tatctggcca tgcggcaggc gacggagttc
     1321 tcggccttcc aggactccgc ccccggcaag atcatgcacg agacccgcgg cggcgagatg
     1381 agcgcgctcg gcgaggtgcc gttcggtctc tactacggcg gtgtcgacac cacctgtctg
     1441 ttcgtcgccc tggccggagc ctacgcccga cggacgggcg acttcgccct gatccgcaag
     1501 ctgtggccca acctgatcgc cgcagtcggc tggatgaagg atcacggcga catccagggc
     1561 gacggcctga tcagctatca gcgcggcgcc gacaccggcc tgtccaatca gggctggaaa
     1621 gactcggaag attcgatctt ccacgccgac ggccggttcc cgaagggccc cgtcgccctg
     1681 ctggaggttc agggctacgc ctacgccgcc tggcaggcca tggccgagct gggcgcggct
     1741 gtgggcgatg ccggagcgct ggaatgggcg gcgcaggccg agcgggtgcg ggccctggtc
     1801 gaggaccggt tctggatgga ggacgaaggc ttctacgcca tcgccctcga cggcgacggc
     1861 gagccatgcc ggtcgatcgg gtccaacgcc ggccacctgc tgttcaccgg cctgccgtcg
     1921 gcggagcgag cgaagcgagt gacgaagcgg ctactgggcg ccgagttccg ctcgggctgg
     1981 ggcgtgcgga cgctggccac cggtcaggcg cggttcaatc cgatgagcta ccacaacggc
     2041 tcggtctggc cgcacgacac ggccatggcc gccgccggca tggcccgcta cggcgagcgc
     2101 cgggctgtgg ccctgctgct gggcgaaatc tacgcctcgg cggcccattt ccacctgcgc
     2161 ctgcccgagc tgttctgcgg tttcgaacgg gcgccgggcg agccgcccat cgcctatccg
     2221 gtcgcctgcc tgccacaggc ctgggcggcg ggatcggtgt tcctgatgct gcaggccgca
     2281 ctgggggtga acatcgacgc ggtggccggg accgtcgaga tcgacgatcc ggtgctgccg
     2341 tcgggcatcg accggctgaa cgtcaccaat ctgcaggtcg gcgacggcgt cgtggatctg
     2401 gccttccagc agatggg
//
LOCUS       DASVVN010000669         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_261772,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000669 DASVVN010000000
VERSION     DASVVN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261772"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF686_10990"
     CDS             <1..238
                     /locus_tag="VF686_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEX8662381.1"
                     /translation="LVRDGQPAADLEVTIARGGTRYRDNPEEMTVRTSADGAFTVTWP
                     EAGMYWMNASVRTPASGSQMASSAAYNGVVEVLP"
     gene            391..1305
                     /locus_tag="VF686_10995"
     CDS             391..1305
                     /locus_tag="VF686_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX8662382.1"
                     /translation="MGTSWAVRLIPPPGLDRAAFQTAIEAELAAIIALFSHWDPTSEL
                     ARFNAAPPGFWAVSEPFWALLNTALDLADDTNGSVDPTLGALVDLWGFGPPGPRPDVP
                     SDDEVAAALTVSGWGALRLNREARAVQQPGGLKLDLSGIAKGHAVDRVSERLTAMGAV
                     HHLVEIGGELRGAGVKPGGHPWWVELQQAPGTPAPRTAAALFDMAVATSGDWVRRFEA
                     GGRTYSHTIDGHTGRPVDNGVASVTVLADTALYADAMATALTVMGRYDGPAYAEALNI
                     AAAFVVRTETGLLEIVSPAFAAMAAEGA"
     gene            1302..2669
                     /locus_tag="VF686_11000"
     CDS             1302..2669
                     /locus_tag="VF686_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEX8662383.1"
                     /translation="MTADPVRWLWTAAAVALWLLVVGAVAWSRARSRRAAAKRAGALA
                     SGADAVTVAFASQTGLAEELAWMTARSLSDAGTGARVVPLGDLDAAALKGCGRLLIVA
                     STTGEGDAPDGVSRFLRQTMSAPADLSGLGYGLLALGDHTYADFCGFGRALDSWLQRS
                     GAEPLFDRVEVDNGDAGAIRHWQHQLGLLTGSTIQADWTPPAYDRWTLIERRHLNPGS
                     PGGEAWLLAFAPAGHQPMWAAGDIAEIGLPASADGTDPGSREYSVASLPGDGRAEFIV
                     RRMTRPDGSPGLASGWLTHGLAVGDEVGMRIRANRSFHGPAAGTPLVLIGNGTGLAGL
                     RAHWKARAGRPHGGVWLMFGERTRAHDAFLDDELQAALTSRDLTRLDRVFSRDATDGR
                     YVQALIAEHADDLRAWVDRGAAILVCGSLEGMSKGVQAALTDALGAERLAELTEAGRY
                     RRDVY"
     gene            complement(2673..3350)
                     /locus_tag="VF686_11005"
     CDS             complement(2673..3350)
                     /locus_tag="VF686_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX8662384.1"
                     /translation="MLLHIPDVFAKDEVAALRARLDAGPWVDGNVTSGHQSAGAKVNR
                     QLPEDCAEAREVGALIQQALNANPMFVSAALPHTVFPPLFNRYEGGERFGLHVDNAIR
                     QRGAIRIRSDLSATLFLSEPDDYDGGELIIEEMYGPQSVKLPAGDLVLYPSKSLHSVT
                     PVTRGARAASFFWIQSLVRDDADRETLFRLDVAIQRVSADKGPQDVAVLELTAVYHNL
                     LRRWAEA"
     gene            complement(3466..>4054)
                     /locus_tag="VF686_11010"
     CDS             complement(3466..>4054)
                     /locus_tag="VF686_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8662385.1"
                     /translation="DRLAVSGAAFLLTRENAAILVGPGPADFQQAGEVEVRGIELGVS
                     GNITRQWQVFGGYTWMDSELVKGAVTVVGTPPVVVPNPFEGLPLANTPEHTFSLFTTY
                     RVTPKLSLGGGFYYVSDSFGGNQGGAGGGANRVYAPAYTRFDLFASYDLNETASLQLN
                     VQNATDEEYIMRTNGVHHADVAPARQAILTLNLRY"
BASE COUNT          584 a         1478 c         1392 g          600 t
ORIGIN      
        1 actggtccgc gatggccagc cggcggcgga tctggaggtg accatcgccc gcggcggcac
       61 gcgctatcgc gacaatcccg aagaaatgac ggtccgcacc agcgccgacg gcgccttcac
      121 cgtcacctgg cccgaggccg gcatgtactg gatgaacgcc tcggtccgca ccccggcctc
      181 gggttcgcag atggcctcaa gcgccgccta caacggcgtc gtggaagtcc tgccctgacc
      241 ggaacgccgc ccggcccgcg cggcgcgccg ccgccgatca ccatcggcga ggcggcggcg
      301 cagccggaga accgcgtcct gattccgacg cacggccgga cgcccgagct gccgacgtcc
      361 gacctgctct gggacttcgc cggcgagacc atgggcacca gctgggccgt ccggctgatc
      421 ccgccgccgg gactggatcg cgccgcgttc cagacggcga tcgaggccga actggcggcg
      481 atcatcgcct tgttcagcca ttgggacccg acctcggaac tggcccggtt caacgctgct
      541 ccacccggat tctgggcggt gtcggagccg ttctgggcgt tgctgaacac cgccctggat
      601 ctggccgacg acaccaacgg ctcggtcgat ccgacattgg gcgcgctggt cgacctgtgg
      661 ggcttcggcc cgccgggacc gcggccggat gttccttccg acgacgaagt cgcggcggcc
      721 ctgaccgtca gcggctgggg cgcactgcgg ctgaaccgcg aggcccgcgc ggtccagcag
      781 ccgggcggcc tcaaactgga tctgtccggc atcgccaagg gccatgccgt cgaccgggtt
      841 tccgaacgcc tgaccgccat gggcgccgtt caccatctgg tcgagatcgg cggcgagctg
      901 cgcggcgccg gcgtcaaacc gggcggccac ccatggtggg tcgagttgca acaggcgccc
      961 ggaacccccg ccccccgcac cgccgccgcc ctgttcgaca tggccgtcgc cacctcgggc
     1021 gactgggtcc gccggttcga ggccggcggt cggacgtaca gccacaccat cgacggccac
     1081 accggccggc ccgtcgacaa cggcgtcgcc tcggtgaccg ttctggccga caccgccctg
     1141 tacgccgacg ccatggccac ggccctgacg gtcatgggcc gctacgacgg tccggcctac
     1201 gccgaggcct tgaacatcgc cgccgccttc gtcgtgcgca ctgaaaccgg cctgctcgag
     1261 atcgtctccc cggccttcgc cgccatggcc gccgagggcg cgtgaccgcc gatcccgtac
     1321 gctggctgtg gacggccgcg gccgtcgccc tgtggctgct ggtcgtcggc gccgtcgcat
     1381 ggagtcgggc ccgaagccgc cgcgccgctg cgaaacgcgc cggcgcgctc gcctccggcg
     1441 ccgacgcggt caccgtcgcc ttcgccagcc agacgggcct cgccgaggaa ctggcctgga
     1501 tgacggcgcg cagcctctcc gacgccggaa ccggcgcgcg ggtggttcct ctgggcgacc
     1561 tcgacgctgc ggcgctcaaa gggtgcggcc ggttgctgat cgtcgcctcc acgaccggag
     1621 agggcgacgc gcccgacggc gtgtcccgct tcttgcgcca gacgatgagc gcgcctgccg
     1681 acctgtccgg ccttggctac ggcctgctgg cgctgggcga tcacacctat gcggacttct
     1741 gcggcttcgg ccgtgcgctc gacagctggc ttcagcgctc cggggcggag ccactgttcg
     1801 accgcgtcga ggtcgataac ggggacgccg gcgccatccg ccactggcag catcaactgg
     1861 gcctgctgac cggctcgacc atccaggccg actggacgcc gcccgcctat gatcgctgga
     1921 ccctgatcga acgccgccac ctcaatccgg gcagtccggg cggggaagcg tggttgctgg
     1981 ccttcgcgcc ggccggtcac cagccgatgt gggcggccgg cgacatcgcc gagatcgggc
     2041 tcccggcctc cgctgacggg acagacccgg gcagccgcga atactccgtc gcctccctgc
     2101 cgggggacgg tcgcgccgag ttcatcgtgc gccgcatgac ccggccggac gggtcgcccg
     2161 gcctcgcctc gggctggctg acccacggcc tcgcggtcgg cgacgaggtc ggcatgcgca
     2221 tacgcgccaa ccgcagtttc catggaccgg cggccgggac gccgctggtg ttgatcggca
     2281 acggcaccgg actggcgggt ctgcgcgccc actggaaggc ccgggcgggt cgcccccacg
     2341 gcggcgtctg gctgatgttc ggcgagcgca cccgcgccca tgacgccttc ctcgacgacg
     2401 aactccaggc ggccctgacg tcgcgcgacc tgacccgtct cgaccgcgtc ttctcacgcg
     2461 acgcaacaga tggccgttat gtacaggccc tgatcgcgga acatgccgac gacctcaggg
     2521 cgtgggtcga ccgcggcgcg gccatcctcg tctgcggcag cctcgaaggc atgtcgaagg
     2581 gggtgcaggc cgctctgacc gatgcgcttg gcgccgaacg gctggcggaa ttgaccgagg
     2641 ccgggcgcta ccgccgcgac gtctactgag cctcaggcct cggcccagcg ccgcagcagg
     2701 ttgtgataga cggccgtcag ctccagcacc gccacgtcct gcggcccctt gtcggcgctg
     2761 acccgctgga tggctacgtc cagccggaac agcgtctcgc ggtccgcgtc gtcgcgcacc
     2821 agactctgga tccagaagaa gctggctgcc cgcgcgccgc gcgtcaccgg agtcacgctg
     2881 tgcaagctct tggaggggta gagcaccaga tccccggccg gcagtttgac actctgcgga
     2941 ccgtacatct cctcgatgat cagctcgccg ccgtcgtagt cgtcgggctc cgacaggaac
     3001 agggtcgccg acaggtcgct gcgaatgcgg atggcgcctc gctggcggat ggcgttgtcg
     3061 acgtgcaggc cgaaccgctc gccgccctca tagcggttga acagcggcgg aaaaaccgta
     3121 tgcggcagcg ccgcggagac gaacatgggg ttggcgttca gcgcctgctg gatcagcgcc
     3181 cccacctcgc gcgcctcggc gcagtcctcg ggcagctggc ggttgacctt ggcccccgcc
     3241 gactgatggc ccgaggtgac atttccatcc acccacggac cggcatccag acgtgctcgc
     3301 agggccgcga cctcgtcttt cgcgaacacg tccggaatat gcagcagcat gcgccgcctc
     3361 cttggtcacg ccggaagtcc ggacgtggaa aaacccgggg atcaggccat cggggaagac
     3421 gcctgacccc cgggttctac gctacccgac cttcgggcga agggcctagt agcgcagatt
     3481 cagcgtcaga atggcctgac gggccggcgc aacgtcggcg tggtgcacgc cgttggtgcg
     3541 catgatgtat tcctcgtcgg tcgcgttctg cacgttcagc tgcagcgagg cggtctcgtt
     3601 caggtcgtat gaggcgaaca ggtcgaaccg cgtataggcc ggcgcataga cgcggtttgc
     3661 cccgccgccg gcgccgccct ggttgccgcc gaagctgtcg gacacatagt agaagccgcc
     3721 gcccaggctc agtttcggcg tcacgcggta ggtcgtgaac aggctgaacg tgtgctcggg
     3781 cgtgttggcg aggggcaggc cttcgaacgg gttgggcacg acaacgggcg gcgttcccac
     3841 aaccgtcacc gcgcccttga ccagttcgga gtccatccag gtgtagccgc cgaacacctg
     3901 ccactggcgg gtaatgttgc cggacacgcc cagttcgatc ccgcggacct cgacctcgcc
     3961 ggcctgctgg aagtcggcgg gtccggggcc gaccaggatc gccgcattct cgcgggtcag
     4021 caggaaggcc gcgcccgaca ccgcgaggcg gtcg
//
LOCUS       DASVVN010000670         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_210254,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000670 DASVVN010000000
VERSION     DASVVN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210254"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..997)
                     /locus_tag="VF686_11015"
     CDS             complement(254..997)
                     /locus_tag="VF686_11015"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8662386.1"
                     /translation="MSVVKTYSGAPYPAITHSALQRRLLRNASHPARKTQADSLRLRA
                     VPLVPEVELFLAEDPVLLWARLEADSGNQLTTPFWATAWTGGQALARYVLDNPEVVAG
                     RRVLDVASGSGLVAIAAVLAGAAEVTANDIDAYAIAAMQANANANGCRIAHDDRDLTG
                     GDGGDAEVILIGDCLYNTDVASLMLPFVERATARGATVLIGDPGRGHAPETMLETLAT
                     YHSPAMGADEYGQFEHSSVLTPVAVVPAV"
     gene            complement(1060..1263)
                     /locus_tag="VF686_11020"
     CDS             complement(1060..1263)
                     /locus_tag="VF686_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8662387.1"
                     /translation="MAVGTVKWFNADKGFGFITQDGGAPDVFAHFSAIASDGYRSLQE
                     NQRVEFDVEQGQKGPQAKNIKVI"
     gene            1590..>2247
                     /locus_tag="VF686_11025"
     CDS             1590..>2247
                     /locus_tag="VF686_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HEX8662388.1"
                     /translation="MAEGVRGRTSMARPYKAIAVAAAAITAVGLSPLVSFADERPAPS
                     APAAAAVDMAPGMLAAMKRDLKLDDRQVRARLATEAGAPVVEKRLRGKLGKTFAGAWI
                     PAGKDKLTVAVTTEAAAAQARAEGADVKLVKRGESDLSAARAKMDKAGKKASKDVRGW
                     YVDVATNSVVVMAKPGAEAAAKKFAKDAGAGSVTVVAAAEQPKPMYDIRGGDQYVING
                     N"
BASE COUNT          381 a          778 c          771 g          317 t
ORIGIN      
        1 accatctcgt ccggggtgac cgcgacggcg tcgcagtcga tccggttgat ctcgtccttg
       61 aggcccagca cggtcacgtc gcgcgaggcg gcccagcgga cgatcgcgtc gatcgccgcg
      121 ccgcagtccc gccgcggatg caggaccagc ccgaccgtct tgaccatgcc catcggaccc
      181 acccgtcgct cgtcgcctgt accgggccag ccttacacgt ccgggcccgc ggcggcgagt
      241 ggcgtcccgg ccgtcagacc gcgggaacga cggcgaccgg ggtcagcaca ctactgtgct
      301 cgaactggcc gtactcgtcc gcacccatcg caggagaatg gtaggtcgcg agtgtttcca
      361 gcatggtctc cggcgcgtgg ccgcgaccgg gatcaccgat cagcacggtg gcgccgcggg
      421 cggtggcccg ctcgacgaac ggcaacatga gcgaggcgac gtcggtgttg tagaggcagt
      481 cgccgatcag gatcacctcg gcgtcaccgc cgtcgccgcc ggtgagatcg cggtcgtcat
      541 gggcgatgcg gcagccgttg gcgttggcgt tggcctgcat ggcggcgatc gcgtaggcgt
      601 cgatgtcgtt ggccgtgacc tcggcggcgc cggccagcac cgcggcgatg gccaccagac
      661 cggagccgga ggcgacgtcc aggacccgcc ggccggcgac cacctcgggg ttgtccagca
      721 cgtagcgggc caaggcctgt ccgccggtcc aggcggtcgc ccagaacggc gtggtcagct
      781 ggttgccgct gtccgcctcg agccgcgccc agagcagcac cgggtcctcg gcgaggaaga
      841 gctcgacctc ggggaccagc gggaccgcgc gcagccgcag cgagtcggcc tgggtcttac
      901 gcgccggatg ggaggcgtta cgcaggagac gacgctggag cgcgctgtgg gtaatggccg
      961 gatagggcgc accggaatag gtcttcacaa cgctcatctc ctggtaggga agggaatgga
     1021 aagggggcca cgatgatcgc ggcccccttt gtcaaaacgt cagatcacct tgatgttctt
     1081 cgcctgcggg cccttctgac cctgctcgac gtcgaactcg acccgctggt tctcctggag
     1141 gctccggtac ccgtccgagg cgatggccga gaagtgggcg aacacgtccg gcgcaccgcc
     1201 gtcctgggtg atgaagccga agcccttgtc cgcgttgaac cacttcacag tgccgacagc
     1261 catgtcgcac cctttcgact gctgtaggaa tccacacacc gcggaccccg tcgcgaaatc
     1321 gtgcccgaac acaacaaacg cccggtaggt ctgataccca ccaggcgctg gcaaatatgc
     1381 ggttcgagaa cggcgacggg acagacatta ccacgccaac cggccacggc ctgttcagcc
     1441 gccgatacgc atagatcaac aacgattgaa caggtttctt gacagtacgc cggactatcg
     1501 acagactccg atgtcacgag agcgctctct gtccccacga tcatcccctg agttcagcac
     1561 gcgacgccgc gtcccgcccc tacccggacg tggcggaagg cgtgcgtgga aggacttcca
     1621 tggcccgtcc gtacaaggcg atcgcagtcg ccgcggcggc gatcaccgcg gtgggcttgt
     1681 cgccgctggt gagcttcgcc gacgagcgac ccgccccctc cgccccggcc gccgccgccg
     1741 tcgacatggc ccccggcatg ctcgccgcga tgaagcgcga cctcaagctc gacgaccgcc
     1801 aggtccgcgc ccggctcgcc accgaggcag gtgccccggt ggtcgagaag cggctgcgcg
     1861 gcaagctcgg caagacgttc gccggggcct ggatcccggc gggcaaggac aagctcacgg
     1921 tcgcggtgac caccgaggcg gcggccgcac aggcccgcgc cgagggcgcc gacgtcaagc
     1981 tcgtcaagcg cggggagtcg gacctgtccg ccgcccgcgc caagatggac aaggccggca
     2041 agaaggcctc caaggatgtc cgcggctggt atgtggacgt tgccacgaac agcgtcgtgg
     2101 tgatggccaa gccgggcgcc gaggcggccg ccaagaagtt cgccaaggac gccggggccg
     2161 ggtcggtcac ggtggtggcc gcggccgagc agcccaagcc gatgtacgac atccgcggcg
     2221 gcgaccagta cgtgatcaac ggcaaca
//
LOCUS       DASVVN010000671         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3344 k141_105164,
            whole genome shotgun sequence.
ACCESSION   DASVVN010000671 DASVVN010000000
VERSION     DASVVN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959964
            Sequence Read Archive: SRR10954975
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:04:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,207
            CDSs (total)                      :: 2,183
            Genes (coding)                    :: 2,173
            CDSs (with protein)               :: 2,173
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105164"
                     /isolate="SMAG_U3344"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VF686_11030"
     CDS             <1..763
                     /locus_tag="VF686_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEX8662389.1"
                     /translation="MKKLGVGQPQTTTLEERREGAVRRGNALFIPALIVPLVAVGGTL
                     AAKHTGAGAWLLSPVQTTLIALGLGCLIALFAAMAWLRPPVMAPVQEGRRLMDSIGWA
                     AVLPQMLASLGAVFLASDVGGVVGQLVSQAVPLTSPLLAVVVYCLGMALFTVIMGNAF
                     AAFPVMTAAIGLPFVVVQFGGNPAIVCAIGMLAGFCGTLMTPMAANFNVVPANLLELP
                     DRDSALNGVIRAQIPTALILLVVNMALMYAFAFRF"
     gene            765..>1712
                     /locus_tag="VF686_11035"
     CDS             765..>1712
                     /locus_tag="VF686_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX8662390.1"
                     /translation="MTHRLDAATASRFASAALGHVVREYPNKMDHVLSGPEDVQGPRA
                     LHPIFFGSFDWHSCVHGWWTLFTLLRLYPDMPEAGRIRALADEMFTPANVAAETAYLD
                     RPGSRGFERPYGWAWLLMLGAELERHDDARADTLRPLTQAFARRFTDFLPLADYPVRV
                     GTHYNTAFALRLTLDYAEGAGDAALTTLCRERALHWHSGDRDCQAWEPSQDEFLSPAL
                     MEAVLMRRVMEPDAFAGWFDAFLPRLDRREPATLFQPAGVSDRSDGKIAHLDGLSLSR
                     AWCWREIAAALPSNDPRRVIALKAADDHLDAALPHVTGDY"
BASE COUNT          227 a          597 c          587 g          301 t
ORIGIN      
        1 gatgaagaag ctgggtgtcg gccagccgca gacgaccacg ttggaagagc ggcgcgaggg
       61 cgccgtgcga cgcggcaacg ccctgtttat cccggccctg atcgtgccgc tggtggcggt
      121 cggcggcaca ttggcggcca agcacacggg cgcgggcgca tggctgctat ccccggtcca
      181 gacgacgctg atcgccctcg gactcggctg cctcatcgcc ctgttcgccg ccatggcctg
      241 gctcaggccg ccggtgatgg cgccggtgca ggagggccgg cggctgatgg actcgatcgg
      301 ctgggcggcg gtgctgccgc agatgctggc ctcgctgggg gcggtgtttc tggcgtcaga
      361 cgtgggcggg gtggtgggcc agctggtcag tcaggccgtg cccctgacgt cgcccctgct
      421 ggcggtcgtc gtctattgtc tgggcatggc gctgttcacc gtcatcatgg gcaacgcctt
      481 cgccgccttc ccggtgatga ccgccgcgat cggcctgccg ttcgtggtcg tccagttcgg
      541 aggcaatccc gccatcgtct gcgccatcgg catgctggcc ggcttctgcg gaacgctgat
      601 gacgcccatg gccgccaatt tcaacgtcgt gccggccaat ctgctcgagc ttcccgaccg
      661 ggactccgcg ctgaacggcg tgatccgggc ccagattccg accgccctga tcctgctcgt
      721 ggtcaatatg gcgctgatgt acgccttcgc tttcaggttc tgagatgacg caccgcctcg
      781 acgccgccac cgcctcccgc ttcgcctcgg cggcgctggg ccatgtggtc cgcgaatatc
      841 cgaacaagat ggaccacgtc ctgtccggac ccgaggacgt tcagggcccg cgcgccctgc
      901 acccgatctt cttcggcagt ttcgactggc attcgtgcgt ccacggatgg tggacgctgt
      961 tcaccctgtt gcggctgtat cccgacatgc cggaggccgg gcggatccgg gcgctggccg
     1021 acgagatgtt cacgcccgcc aatgtcgccg ccgagacggc ctatctggat cgtccgggaa
     1081 gccgggggtt cgagcgtccc tacggctggg cgtggctgct gatgctgggc gccgagctgg
     1141 aacggcacga cgatgcccgc gccgatacgc tgcgtccgct gacgcaggcc ttcgcccggc
     1201 ggttcaccga cttcctgccg ctggcggatt atccggtgcg cgtcggaacc cactacaaca
     1261 ccgccttcgc cctgaggctg acgctcgact acgccgaggg tgcgggcgac gcggcgctga
     1321 ccaccctttg ccgggaacgg gcgctgcact ggcactcagg cgaccgcgac tgtcaggcgt
     1381 gggagccgtc gcaggacgaa ttcctgtctc cggccctgat ggaggcggtg ctgatgcgta
     1441 gggtcatgga gcccgacgca ttcgccggct ggttcgacgc cttcctgccc cggctggacc
     1501 ggcgtgaacc ggcgaccctg ttccagcccg ccggcgtcag cgaccggtcg gacggcaaga
     1561 tcgcacatct ggacggcctg agcctgagcc gggcgtggtg ctggcgcgag atcgcggccg
     1621 ccctgccgtc aaacgatcct cgccgggtca tcgctctcaa ggccgccgac gaccacctcg
     1681 acgccgccct gccccacgtc acgggcgact at
//
